White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Lymphocyte Culture Test, Mixed
Measure of histocompatibility at the HL-A locus. Peripheral blood lymphocytes from two individuals are mixed together in tissue culture for several days. Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly (measured by tritiated thymidine uptake) whereas those from compatible individuals will not. In the one-way MLC test, the lymphocytes from one of the individuals are inactivated (usually by treatment with MITOMYCIN or radiation) thereby allowing only the untreated remaining population of cells to proliferate in response to foreign histocompatibility antigens.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Antigens, Differentiation, T-Lymphocyte
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Cytotoxicity Tests, Immunologic
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Killer Cells, Natural
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Lymphocyte Function-Associated Antigen-1
An integrin heterodimer widely expressed on cells of hematopoietic origin. CD11A ANTIGEN comprises the alpha chain and the CD18 antigen (ANTIGENS, CD18) the beta chain. Lymphocyte function-associated antigen-1 is a major receptor of T-CELLS; B-CELLS; and GRANULOCYTES. It mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by NATURAL KILLER CELLS and granulocytes. Intracellular adhesion molecule-1 has been defined as a ligand for lymphocyte function-associated antigen-1.
Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
Receptors, Lymphocyte Homing
Cell surface glycoproteins on lymphocytes and other leukocytes that mediate adhesion to specialized blood vessels called high endothelial venules. Several different classes of lymphocyte homing receptors have been identified, and they appear to target different surface molecules (addressins) on high endothelial venules in different tissues. The adhesion plays a crucial role in the trafficking of lymphocytes.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Receptors, Antigen, T-Cell, alpha-beta
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Receptors, Antigen, T-Cell, gamma-delta
T-cell receptors composed of CD3-associated gamma and delta polypeptide chains and expressed primarily in CD4-/CD8- T-cells. The receptors appear to be preferentially located in epithelial sites and probably play a role in the recognition of bacterial antigens. The T-cell receptor gamma/delta chains are separate and not related to the gamma and delta chains which are subunits of CD3 (see ANTIGENS, CD3).
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Amino Acid Sequence
Histocompatibility Antigens Class I
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
A calcium-dependent pore-forming protein synthesized in cytolytic LYMPHOCYTES and sequestered in secretory granules. Upon immunological reaction between a cytolytic lymphocyte and a target cell, perforin is released at the plasma membrane and polymerizes into transmembrane tubules (forming pores) which lead to death of a target cell.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
CD4 Lymphocyte Count
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Immune Adherence Reaction
A method for the detection of very small quantities of antibody in which the antigen-antibody-complement complex adheres to indicator cells, usually primate erythrocytes or nonprimate blood platelets. The reaction is dependent on the number of bound C3 molecules on the C3b receptor sites of the indicator cell.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). This is usually considered a form of passive immunotherapy. (From DeVita, et al., Cancer, 1993, pp.305-7, 314)
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Receptors, Antigen, B-Cell
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Pore Forming Cytotoxic Proteins
Proteins secreted from an organism which form membrane-spanning pores in target cells to destroy them. This is in contrast to PORINS and MEMBRANE TRANSPORT PROTEINS that function within the synthesizing organism and COMPLEMENT immune proteins. These pore forming cytotoxic proteins are a form of primitive cellular defense which are also found in human LYMPHOCYTES.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Fas Ligand Protein
A transmembrane protein belonging to the tumor necrosis factor superfamily that was originally discovered on cells of the lymphoid-myeloid lineage, including activated T-LYMPHOCYTES and NATURAL KILLER CELLS. It plays an important role in immune homeostasis and cell-mediated toxicity by binding to the FAS RECEPTOR and triggering APOPTOSIS.
Histocompatibility Antigens Class II
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Tumor Cells, Cultured
Major Histocompatibility Complex
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Dose-Response Relationship, Immunologic
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Gene Expression Regulation
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Immunologic Deficiency Syndromes
Herpesvirus 4, Human
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
This enzyme is a lymphoid-specific src family tyrosine kinase that is critical for T-cell development and activation. Lck is associated with the cytoplasmic domains of CD4, CD8 and the beta-chain of the IL-2 receptor, and is thought to be involved in the earliest steps of TCR-mediated T-cell activation.
Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.
Cell Line, Transformed
Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Cell Adhesion Molecules
Subset of helper-inducer T-lymphocytes which synthesize and secrete interleukin-2, gamma-interferon, and interleukin-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.
Tumor Necrosis Factor-alpha
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Receptor-CD3 Complex, Antigen, T-Cell
Molecule composed of the non-covalent association of the T-cell antigen receptor (RECEPTORS, ANTIGEN, T-CELL) with the CD3 complex (ANTIGENS, CD3). This association is required for the surface expression and function of both components. The molecule consists of up to seven chains: either the alpha/beta or gamma/delta chains of the T-cell receptor, and four or five chains in the CD3 complex.
Graft vs Host Reaction
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Cell surface glycoproteins that bind to chemokines and thus mediate the migration of pro-inflammatory molecules. The receptors are members of the seven-transmembrane G protein-coupled receptor family. Like the CHEMOKINES themselves, the receptors can be divided into at least three structural branches: CR, CCR, and CXCR, according to variations in a shared cysteine motif.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
An energy dependent process following the crosslinking of B CELL ANTIGEN RECEPTORS by multivalent ligands (bivalent anti-antibodies, LECTINS or ANTIGENS), on the B-cell surface. The crosslinked ligand-antigen receptor complexes collect in patches which flow to and aggregate at one pole of the cell to form a large mass - the cap. The caps may then be endocytosed or shed into the environment.
Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.
Bone Marrow Cells
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Disease Models, Animal
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Simian immunodeficiency virus
T-cell development: a new marker of differentiation state. (1/39207)Differentiation of T cells is a complicated affair and there has been a dearth of markers that faithfully reflect thymocyte phenotype. A new strategy based on T-cell receptor gene sequencing has revealed a marker that can be used to monitor thymocyte differentiation with fidelity and without perturbation. (+info)
The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors. (2/39207)BACKGROUND: The adaptor protein Gads is a Grb2-related protein originally identified on the basis of its interaction with the tyrosine-phosphorylated form of the docking protein Shc. Gads protein expression is restricted to hematopoietic tissues and cell lines. Gads contains a Src homology 2 (SH2) domain, which has previously been shown to have a similar binding specificity to that of Grb2. Gads also possesses two SH3 domains, but these have a distinct binding specificity to those of Grb2, as Gads does not bind to known Grb2 SH3 domain targets. Here, we investigated whether Gads is involved in T-cell signaling. RESULTS: We found that Gads is highly expressed in T cells and that the SLP-76 adaptor protein is a major Gads-associated protein in vivo. The constitutive interaction between Gads and SLP-76 was mediated by the carboxy-terminal SH3 domain of Gads and a 20 amino-acid proline-rich region in SLP-76. Gads also coimmunoprecipitated the tyrosine-phosphorylated form of the linker for activated T cells (LAT) adaptor protein following cross-linking of the T-cell receptor; this interaction was mediated by the Gads SH2 domain. Overexpression of Gads and SLP-76 resulted in a synergistic augmentation of T-cell signaling, as measured by activation of nuclear factor of activated T cells (NFAT), and this cooperation required a functional Gads SH2 domain. CONCLUSIONS: These results demonstrate that Gads plays an important role in T-cell signaling via its association with SLP-76 and LAT. Gads may promote cross-talk between the LAT and SLP-76 signaling complexes, thereby coupling membrane-proximal events to downstream signaling pathways. (+info)
JNK2 is required for efficient T-cell activation and apoptosis but not for normal lymphocyte development. (3/39207)BACKGROUND: The Jun N-terminal kinase (JNK) signaling pathway has been implicated in cell proliferation and apoptosis, but its function seems to depend on the cell type and inducing signal. In T cells, JNK has been implicated in both antigen-induced activation and apoptosis. RESULTS: We generated mice lacking the JNK2 isozymes. The mutant mice were healthy and fertile but defective in peripheral T-cell activation induced by antibody to the CD3 component of the T-cell receptor (TCR) complex - proliferation and production of interleukin-2 (IL-2), IL-4 and interferon-gamma (IFN-gamma) were reduced. The proliferation defect was restored by exogenous IL-2. B-cell activation was normal in the absence of JNK2. Activation-induced peripheral T-cell apoptosis was comparable between mutant and wild-type mice, but immature (CD4(+) CD8(+)) thymocytes lacking JNK2 were resistant to apoptosis induced by administration of anti-CD3 antibody in vivo. The lack of JNK2 also resulted in partial resistance of thymocytes to anti-CD3 antibody in vitro, but had little or no effect on apoptosis induced by anti-Fas antibody, dexamethasone or ultraviolet-C (UVC) radiation. CONCLUSIONS: JNK2 is essential for efficient activation of peripheral T cells but not B cells. Peripheral T-cell activation is probably required indirectly for induction of thymocyte apoptosis resulting from administration of anti-CD3 antibody in vivo. JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells. (+info)
Hematopoietic stem-cell transplantation for the treatment of severe combined immunodeficiency. (4/39207)BACKGROUND: Since 1968 it has been known that bone marrow transplantation can ameliorate severe combined immunodeficiency, but data on the long-term efficacy of this treatment are limited. We prospectively studied immunologic function in 89 consecutive infants with severe combined immunodeficiency who received hematopoietic stem-cell transplants at Duke University Medical Center between May 1982 and September 1998. METHODS: Serum immunoglobulin levels and lymphocyte phenotypes and function were assessed and genetic analyses performed according to standard methods. Bone marrow was depleted of T cells by agglutination with soybean lectin and by sheep-erythrocyte rosetting before transplantation. RESULTS: Seventy-seven of the infants received T-cell-depleted, HLA-haploidentical parental marrow, and 12 received HLA-identical marrow from a related donor; 3 of the recipients of haploidentical marrow also received placental-blood transplants from unrelated donors. Except for two patients who received placental blood, none of the recipients received chemotherapy before transplantation or prophylaxis against graft-versus-host disease. Of the 89 infants, 72 (81 percent) were still alive 3 months to 16.5 years after transplantation, including all of the 12 who received HLA-identical marrow, 60 of the 77 (78 percent) who were given haploidentical marrow, and 2 of the 3 (67 percent) who received both haploidentical marrow and placental blood. T-cell function became normal within two weeks after transplantation in the patients who received unfractionated HLA-identical marrow but usually not until three to four months after transplantation in those who received T-cell-depleted marrow. At the time of the most recent evaluation, all but 4 of the 72 survivors had normal T-cell function, and all the T cells in their blood were of donor origin. B-cell function remained abnormal in many of the recipients of haploidentical marrow. In 26 children (5 recipients of HLA-identical marrow and 21 recipients of haploidentical marrow) between 2 percent and 100 percent of B cells were of donor origin. Forty-five of the 72 children were receiving intravenous immune globulin. CONCLUSIONS: Transplantation of marrow from a related donor is a life-saving and life-sustaining treatment for patients with any type of severe combined immunodeficiency, even when there is no HLA-identical donor. (+info)
Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation. (5/39207)Cbl-b, a mammalian homolog of Cbl, consists of an N-terminal region (Cbl-b-N) highly homologous to oncogenic v-Cbl, a Ring finger, and a C-terminal region containing multiple proline-rich stretches and potential tyrosine phosphorylation sites. In the present study, we demonstrate that upon engagement of the T cell receptor (TCR), endogenous Cbl-b becomes rapidly tyrosine-phosphorylated. In heterogeneous COS-1 cells, Cbl-b was phosphorylated on tyrosine residues by both Syk- (Syk/Zap-70) and Src- (Fyn/Lck) family kinases, with Syk kinase inducing the most prominent effect. Syk associates and phosphorylates Cbl-b in Jurkat T cells. A Tyr-316 Cbl-binding site in Syk was required for the association with and for the maximal tyrosine phosphorylation of Cbl-b. Mutation at a loss-of-function site (Gly-298) in Cbl-b-N disrupts its interaction with Syk. Cbl-b constitutively binds Grb2 and becomes associated with Crk-L upon TCR stimulation. The Grb2- and the Crk-L-binding regions were mapped to the C-terminus of Cbl-b. The Crk-L-binding sites were further determined to be Y655DVP and Y709KIP, with the latter being the primary binding site. Taken together, these results implicate that Cbl-b is involved in TCR-mediated intracellular signaling pathways. (+info)
Vascular endothelial growth factor activates nuclear factor of activated T cells in human endothelial cells: a role for tissue factor gene expression. (6/39207)Vascular endothelial growth factor (VEGF) is a potent angiogenic inducer that stimulates the expression of tissue factor (TF), the major cellular initiator of blood coagulation. Here we show that signaling triggered by VEGF induced DNA-binding and transcriptional activities of nuclear factor of activated T cells (NFAT) and AP-1 in human umbilical vein endothelial cells (HUVECs). VEGF also induced TF mRNA expression and gene promoter activation by a cyclosporin A (CsA)-sensitive mechanism. As in lymphoid cells, NFAT was dephosphorylated and translocated to the nucleus upon activation of HUVECs, and these processes were blocked by CsA. NFAT was involved in the VEGF-mediated TF promoter activation as evidenced by cotransfection experiments with a dominant negative version of NFAT and site-directed mutagenesis of a newly identified NFAT site within the TF promoter that overlaps with a previously identified kappaB-like site. Strikingly, this site bound exclusively NFAT not only from nuclear extracts of HUVECs activated by VEGF, a stimulus that failed to induce NF-kappaB-binding activity, but also from extracts of cells activated with phorbol esters and calcium ionophore, a combination of stimuli that triggered the simultaneous activation of NFAT and NF-kappaB. These results implicate NFAT in the regulation of endothelial genes by physiological means and shed light on the mechanisms that switch on the gene expression program induced by VEGF and those regulating TF gene expression. (+info)
Activation-dependent transcriptional regulation of the human Fas promoter requires NF-kappaB p50-p65 recruitment. (7/39207)Fas (CD95) and Fas ligand (CD95L) are an interacting receptor-ligand pair required for immune homeostasis. Lymphocyte activation results in the upregulation of Fas expression and the acquisition of sensitivity to FasL-mediated apoptosis. Although Fas upregulation is central to the preservation of immunologic tolerance, little is known about the molecular machinery underlying this process. To investigate the events involved in activation-induced Fas upregulation, we have examined mRNA accumulation, fas promoter activity, and protein expression in the Jurkat T-cell line treated with phorbol myristate acetate and ionomycin (P/I), pharmacological mimics of T-cell receptor activation. Although resting Jurkat cells express Fas, Fas mRNA was induced approximately 10-fold in 2 h upon P/I stimulation. Using sequential deletion mutants of the human fas promoter in transient transfection assays, we identified a 47-bp sequence (positions -306 to -260 relative to the ATG) required for activation-driven fas upregulation. Sequence analysis revealed the presence of a previously unrecognized composite binding site for both the Sp1 and NF-kappaB transcription factors at positions -295 to -286. Electrophoretic mobility shift assay (EMSA) and supershift analyses of this region documented constitutive binding of Sp1 in unactivated nuclear extracts and inducible binding of p50-p65 NF-kappaB heterodimers after P/I activation. Sp1 and NF-kappaB transcription factor binding was shown to be mutually exclusive by EMSA displacement studies with purified recombinant Sp1 and recombinant p50. The functional contribution of the kappaB-Sp1 composite site in P/I-inducible fas promoter activation was verified by using kappaB-Sp1 concatamers (-295 to -286) in a thymidine kinase promoter-driven reporter construct and native promoter constructs in Jurkat cells overexpressing IkappaB-alpha. Site-directed mutagenesis of the critical guanine nucleotides in the kappaB-Sp1 element documented the essential role of this site in activation-dependent fas promoter induction. (+info)
RFLAT-1: a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes. (8/39207)RANTES (Regulated upon Activation, Normal T cell Expressed and Secreted) is a chemoattractant cytokine (chemokine) important in the generation of inflammatory infiltrate and human immunodeficiency virus entry into immune cells. RANTES is expressed late (3-5 days) after activation in T lymphocytes. Using expression cloning, we identified the first "late" T lymphocyte associated transcription factor and named it "RANTES Factor of Late Activated T Lymphocytes-1" (RFLAT-1). RFLAT-1 is a novel, phosphorylated, zinc finger transcription factor that is expressed in T cells 3 days after activation, coincident with RANTES expression. While Rel proteins play the dominant role in RANTES gene expression in fibroblasts, RFLAT-1 is a strong transactivator for RANTES in T cells. (+info)
Selective inhibition of antigen-specific T lymphocyte proliferation by by Gyongyi Szabo, Bikash K. Verma et al.
Ethanol consumption is associated with impaired immunity. Our data demonstrate that even a single dose of a biologically relevant concentration (25-150 mM) of ethanol can down-regulate antigen-specific T lymphocyte proliferation. In contrast, ethanol augmented mitogen-induced T cell proliferation, suggesting that its inhibitory effect on antigen-specific T cell proliferation was due to its effects on monocytes (m phi s) rather than on T cells. The immunodepressive effects of ethanol on m phi antigen-presenting cell (APC) capacity were manifested whether alcohol treatment was limited to the antigen uptake-processing period only or was present during the entire period of antigen presentation. These inhibitory effects of ethanol were also evident on both the high-antigen-presenting, Fc gamma RI-negative (-31 +/- 17%), and low-antigen-presenting, Fc gamma RI-positive (-42 +/- 15%) m phi subpopulations. Further analysis demonstrated that ethanol inhibits the production of interleukin-1 beta (IL-1 beta) and
Immortalization of Human and Rhesus Macaque Primary Antigen‐Specific T Cells by Retrovirally Transduced Telomerase Reverse...
Human and rhesus macaque primary antigen‐specific T cells derived from infected or immunized individuals or animals are a valuable material with which to study cellular immune responses against pathogens and tumors
NanoString Technologies, Inc. :: Simultaneous multi-analyte profiling of rare antigen specific T cells with 3D Flow™ Analysis
In this webinar, we feature Dr. Karen Anderson who utilized 3D Flow Analysis to deeply profile antigen specific T cells. Hear about the exciting biology uncovered from these rare cells utilizing this novel technique.
Inflammatory T-lymphocyte proliferation in morbid obesity | Journal of Translational Medicine | Full Text
We found a selective increase in T-lymphocyte number in morbid obese subjects, which was mainly caused by an increase in CD4+ T-lymphocytes. Also, in morbid obese subjects TREC content was decreased in all T-lymphocyte subpopulations, demonstrating that the increase in T-lymphocytes is mainly caused by increased proliferation. Moreover, in morbid obese subjects we found increased plasma levels of IL-7 and CCL5, both potent enhancers of T-lymphocyte proliferation. Also, plasma of morbid obese subjects enhanced T-lymphocyte proliferation in vitro.. Finally, both CD4+ and CD8+ T-lymphocytes had some skewing of the TCR repertoire. ...
Cyclosporin A inhibits initiation but not progression of human T cell proliferation triggered by phorbol esters and calcium...
TY - JOUR. T1 - Cyclosporin A inhibits initiation but not progression of human T cell proliferation triggered by phorbol esters and calcium ionophores. AU - Kumagai, N.. AU - Benedict, S. H.. AU - Mills, G. B.. AU - Gelfand, E. W.. PY - 1988/12/1. Y1 - 1988/12/1. N2 - Cyclosporin A (CsA) is a potent inhibitor of T lymphocyte proliferation induced by Ag and mitogens. In an attempt to further delineate the mechanism of action of CsA, we have examined its effects on T cell proliferation induced by the combination of the phorbol ester, phorbol 12,13-dibutyrate (PDB), and the calcium ionophore, ionomycin. T cells were rendered competent as the result of a 30-min initial incubation with both drugs, after which the drugs were washed out. Competence is defined as the ability to subsequently proliferate in response to exogenously added IL-2 or PDB in the second phase of the culture, but not to synthesize IL-2 or proliferative without these additions. Addition of CsA (1 μg/ml) to the cells in the ...
Phenotypic Analysis of Antigen-Specific T Lymphocytes | Science
Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens. ...
A novel model for antigen-dependent activation of normal human T cells. Transmembrane signaling by crosslinkage of the CD3/T...
Transmembrane signaling of normal human T cells was explored with mAbs directed at TCR, CD2, CD4, CD5, or CD8 antigens and highly purified CD4+ T cells and CD8+ T cells. Our experiments explicitly show that: (a) crosslinkage of TCR with the CD2 antigen, and not independent crosslinking of TCR and of CD2 antigen or crosslinking of either protein with the CD4 or CD8 antigen induces significant proliferation independent of co-stimulatory signals (e.g., accessory cells, recombinant lymphokines, or tumor promoter), (b) F(ab)2 fragments of mAb directed at the TCR and F(ab)2 anti-CD2, crosslinked with F(ab)2 fragments of rabbit anti-mouse IgG, promote the proliferation of highly purified T cells, (c) a prompt and sustained increase in intracellular free Ca2+ concentration results from crosslinkage of TCR with the CD2 antigen, (d) T cell proliferation induced by this novel approach is curtailed by EGTA and by direct or competitive inhibitors of PKC, (e) crosslinkage of TCR with the CD2 antigen ...
Costimulatory molecules in human atherosclerotic plaques: an indication of antigen specific T lymphocyte activation
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. ...
Application of the pMHC array to characterise tumour antigen specific T cell populations in leukaemia patients at disease...
Immunotherapy treatments for cancer are becoming increasingly successful, however to further improve our understanding of the T-cell recognition involved in effective responses and to encourage moves towards the development of personalised treatments for leukaemia immunotherapy, precise antigenic targets in individual patients have been identified. Cellular arrays using peptide-MHC (pMHC) tetramers allow the simultaneous detection of different antigen specific T-cell populations naturally circulating in patients and normal donors. We have developed the pMHC array to detect CD8+ T-cell populations in leukaemia patients that recognise epitopes within viral antigens (cytomegalovirus (CMV) and influenza (Flu)) and leukaemia antigens (including Per Arnt Sim domain 1 (PASD1), MelanA, Wilms Tumour (WT1) and tyrosinase). We show that the pMHC array is at least as sensitive as flow cytometry and has the potential to rapidly identify more than 40 specific T-cell populations in a small sample of T-cells ...
Production and Concentration of Lentivirus for Transduction of Primary Human T Cells. - Physiology, Anatomy and Genetics
Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell types. However, despite this advantage, the ability to transduce primary human T cells remains challenging and methods to achieve efficient gene transfer are often time consuming and expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility.
MHC‐Peptide Tetramers to Visualize Antigen‐Specific T Cells - Current Protocols
Mature T lymphocytes of the CD8 or CD4 classes bear αβ T cell receptors (TCR) that are specific for a molecular complex consisting of a major histocompatibility complex class I or II (MHC class I or II) molecule bound to a unique self or foreign peptide
PLOS ONE: Overnight Resting of PBMC Changes Functional Signatures of Antigen Specific T- Cell Responses: Impact for Immune...
Polyfunctional CD4 or CD8 T cells are proposed to represent a correlate of immune control for persistent viruses as well as for vaccine mediated protection against infection. A well-suited methodology to study complex functional phenotypes of antiviral T cells is the combined staining of intracellular cytokines and phenotypic marker expression using polychromatic flow cytometry. In this study we analyzed the effect of an overnight resting period at 37°C on the quantity and functionality of HIV-1, EBV, CMV, HBV and HCV specific CD4 and CD8 T-cell responses in a cohort of 21 individuals. We quantified total antigen specific T cells by multimer staining and used 10-color intracellular cytokine staining (ICS) to determine IFNγ, TNFα, IL2 and MIP1β production. After an overnight resting significantly higher numbers of functionally active T cells were detectable by ICS for all tested antigen specificities, whereas the total number of antigen specific T cells determined by multimer staining remained
Mesenchymal Stem Cells Exert Differential Effects on Alloantigen and Virus-Specific T-cell Responses
Mesenchymal stem cells (MSCs) suppress alloantigen-induced T-cell functions in vitro and infusion of third-party MSCs seems to be a promising therapy for graft-versus-host disease (GVHD).
CD8-APC-A700 - Beckman Coulter
The CD8 antigen is a disulfide-linked dimer, which exists either as a CD8α homodimer or as a CD8α/β heterodimer. CD8α is required for surface expression of CD8β. The molecular weight of each monomer of α or β is approximately 32-34 kDa. CD8 binds to a non-polymorphic domain (α3 domain) of MHC Class I molecules. CD8 is expressed on a subset of human peripheral blood T lymphocytes. A subset of NK cells possess the CD8 antigen but show low to medium density of expression. CD8α homodimer is expressed by NK cells and γ/δ+ T cells. CD8 is also present on most thymocytes where it is frequently co-expressed with CD4, and on a subpopulation of bone marrow cells. The CD8 molecule acts with the T Cell Receptor (TCR) as a coreceptor for MHC class I restricted antigen recognition. CD8 is widely used as a marker of cytotoxic T lymphocytes. *Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc ...
Effect of three kinds of anaesthetic drugs on postoperative recovery, regulatory T cells and T lymphoid cells in elderly...
Effect of three kinds of anaesthetic drugs on postoperative recovery, regulatory T cells and T lymphoid cells in elderly patients
IM0442U - Beckman Coulter
The CD2 antigen (LFA-2) is a monomeric 50 kDa glycoprotein. It was formerly described as the sheep red blood cell receptor, causing T-cell rosetting, and has been identified as the ligand for CD58 (LFA-3). It is also a receptor for CD48, CD59 and CD15, which binds to the multimeric form of CD2. CD2 is present on the majority of normal human peripheral blood T lymphocytes and a high percentage of NK cells. It is also expressed by all thymocytes ...
CD24 and thymic generation of myelin antigen specific T cells - Xue-Feng Bai
Experimental autoimmune encephalomyelitis (EAE) is an animal model for human multiple sclerosis (MS). The development of EAE relies on the generation of an auto...
CD24 and thymic generation of myelin antigen specific T cells - Xue-Feng Bai
Experimental autoimmune encephalomyelitis (EAE) is an animal model for human multiple sclerosis (MS). The development of EAE relies on the generation of an auto...
Regulation of Gene Expression of Murine MD-1 Regulates Subsequent T Cell Activation and Cytokine Production | The Journal of...
The data in this paper show that MD-1 is not associated directly with CD80/CD86, DEC205, or OX2 on the membrane of functional DC (Fig. 3⇑). However, inhibition of MD-1 synthesis achieved by incubating DC with the ODN-1 blocked MD-1 synthesis and then indirectly inhibited up-regulation of the costimulatory molecules CD80/CD86 in response to LPS (Fig. 2⇑). These ODN-1-altered DC-stimulated Th2-type cytokine production instead of Th1 cytokines when cultured with allogeneic responder splenocytes (Table I⇑), and proliferation and CTL generation was not seen. Instead, there was generation of cells that could suppress in a secondary test culture the allogeneic response of untreated C3H splenic T cells to unaltered allogeneic DC (Table II⇑). Generation of these suppressor cells was dependent on persistent OX2 expression on the ODN-1-treated DC, because anti-OX2 mAb blocked the effect. The ability of ODN-1-treated DC to enhance allograft survival in vivo via an OX2-dependent pathway (Fig. 4⇑) ...
T-cell chemiluminescence. A novel aspect of T-cell membrane activation studied with a Jurkat tumour cell line. | Harvard...
T-cell chemiluminescence. A novel aspect of T-cell membrane activation studied with a Jurkat tumour cell line. Scand J Immunol. 1989 Aug; 30(2):265-9 ...
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate | Protocol (Translated to Italian)
Un metodo per espandere γδ cellule T dalle cellule mononucleate del sangue periferico (PBMC) è descritta. PBMC cellule derivate γδ T...
The ELISPOT assay: an easily transferable method for measuring cellular responses and identifying T cell epitopes. - Semantic...
Characterization of human leukocyte antigen (HLA) class I restricted epitopes derived from viral pathogens is imperative for formulating therapeutic interventions, as well as for vaccine design and monitoring. Sensitive, easy and cost-effective assays that measure the frequency of antigen-specific T lymphocytes are crucial for evaluating and improving vaccines and therapies. This paper reviews the ELISPOT technique that allows for quantifying HIV-specific T lymphocytes at the single cell level from peripheral blood by detection of antigen-induced cytokine secretion. The assay can be used successfully to quantify T cell immune responses in humans infected with different pathogens and to assess T cell immunogenicity of vaccines in phase I/II and III clinical trials. This review focuses on the ELISPOT methodology and discusses how it can be standardized and potentially used by multiple international laboratories attached to clinical trial sites.
Regeneration of t-cell helper function in zinc deficient adult a/j mic by jardieu P. Depasquale, P J. Fraker et al.
Depasquale, jardieu P.; Fraker, P J.; and Luecke, R W., Regeneration of t-cell helper function in zinc deficient adult a/j mice. Abstr. (1978). Subject Strain Bibliography 1978. 1286 ...
functional immune - Search
functional immune Searched from official sources arount the web with simple content and resource link From any languages in different countries.
Ultra Low Dose Interleukin-2 in Healthy Volunteers - Full Text View - ClinicalTrials.gov
Interleukin 2 (IL-2, aldesleukin) was discovered as a T cell growth factor more than 30 years ago. IL-2 was the first human cytokine used therapeutically. IL-2 induces antigen specific T cells, and two important lymphocyte subsets: regulatory T cells (T-regs) and natural killer cells (NK) cells. T-regs have a critical role in self-tolerance and pathogenesis of autoimmune disease or graft versus host disease (GVHD), and they have been extensively studied in solid tumors, hematologic malignancies, viral hepatitis, and HIV infections. NK cells have a unique role in bridging innate and adaptive immunity. NK cells facilitate hematopoietic stem cell (HSC) engraftment reduce GVHD and increase graft-versus-leukemia (GVL) effects. NK cells have important roles on pathogenesis of malignancies, autoimmune disease and AIDS. Conventional dose IL-2 treatment promotes marked expansion of regulatory T cells, and NK cells but is associated with significant side effects. However, much lower doses of interleukin-2 ...
Cell count | Zellanalys to » Metabolism and Human T Cell Proliferation
The relationship between T-cell metabolism and T cell effector function is little studied for human T cells. A recent publication by K. Renner et al. now reports about investigations of the group with human CD4 and CD8 T cells.. The authors used the CASY, to accurately and reliably determine the cell number and cell volume in proliferation assays and metabolic restriction experiments.. Read the publication here:. Metabolic plasticity of human T cells: Preserved cytokine production under glucose deprivation or mitochondrial restriction, but 2-deoxy-glucose affects effector functions. Kathrin Renner, Anna-Lena Geiselhöringer, Matthias Fante, Christina Bruss, Stephanie Dyer, Gabriele Saeed hammer, Katrin Peter, Katrin singer, Reinhard Andreesen, Petra Hoffmann, Peter Abubakar, Wolfgang Mr, Marina Kreutz.. Eur J Immunol. 2015 Sep;45(9):2504-16. two: 10.1002/eji.201545473. ...
iwCLL 2017| Anti-CD19 CAR-T cell therapy with defined T-cell subsets for ibrutinib-refractory CLL
Maloney began by explaining that CAR-T cells produced from distinct T-cell subsets differ in potency. NSG mice bearing Raji tumors (0.5x10 6tumor cell inoculation; day 0) were treated with human CAR-T cells manufactured from distinct T-cell subsets (on day 7). CAR-T cells produced from CD8 +T CMcells were highly potent ( Sommermeyer et al.2015).. Engineering selected T-cell subsets could enhance potency and allow delivery of the same cell product in all patients, potentially providing more uniform data on dose response and toxicity.. Pre-clinical studies have established that a defined composition of CD8 +T CMderived and CD4 +derived CAR T-cells provides optimal potency.. ...
Essential Requirement of Antigen Presentation by Monocyte Lineage Cells for the Activation of Primary Human γδ T Cells by...
It is suggested that human γδ T cells play significant roles during intracellular infections, such as tuberculosis (20), malaria (21), ehlrichosis (22), and many others (23, 24, 25, 26, 27, 28, 29, 30, 31). Evidence indicates that human T cells bearing Vγ2Vδ2-TCR, which constitute the vast majority of γδ T cells in healthy adults, respond to unique Ags in the extracts and/or supernatants of these bacteria and protozoa and are activated with respect to proliferation, cytokine production, and cytotoxic activity (17). These Ags include nonpeptide molecules such as a wide variety of small organic pyrophosphomonoesters (3, 4, 5, 7) and alkylamines (12) as well as unprocessed protein Ags (32, 33, 34). Although it has been indicated that the response takes place in a Vγ2Vδ2-TCR-dependent manner based on substantial evidence including the TCR gene transfer studies (15), knowledge of how human γδ T cells recognize such molecules has remained elusive.. It was indicated previously that activation ...
CD8A (Cytotoxic & Suppressor T-Cell Marker); Clone C8/468 (Concentrate) - RA0041-C.1 (603) ( Antibodies, Research Antibodies)
Providing CD8A (Cytotoxic & Suppressor T-Cell Marker); Clone C8/468 (Concentrate) - RA0041-C.1 ( Antibodies, Research Antibodies)
DI-fusion Cutting edge: Antigen-specific T lymphocytes regulate...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Monocytes are required to prime peripheral blood T cells to undergo apoptosis. | PNAS
Freshly isolated, human peripheral blood T (PBT) cells are largely resistant to the apoptotic effects of anti-CD3 monoclonal antibody, ionomycin, or phorbol 12-myristate 13-acetate (PMA). We demonstrate here, however, that PBT cells, including both CD4+ and CD8+ cell populations, can be readily induced to undergo apoptosis when cocultured with either autologous or allogeneic monocytes (Mo) in PMA-containing medium. Incubation of PBT cells with Mo at a ratio of 1:1 for 18 hr resulted in maximal levels (80%) of apoptotic cell death. The mechanism whereby Mo enable PBT cells to undergo apoptosis in PMA-containing medium appeared to depend on cell-cell contact or close proximity between Mo and PBT cells rather than solely via soluble mediators. It was demonstrated that Mo acquire the ability to prime PBT cells for apoptosis after treatment with PMA and that treated Mo maintain this ability even after fixation with formaldehyde. It was also found that once PBT cells became primed for apoptosis by ...
Kinetic analysis of immune responses associated with tumor regression - Institut Cochin
Recent clinical trials have demonstrated that a type of immune cell called T lymphocytes may play an important role in inducing the regression of different cancers, in particular of blood cancers. Other immune cells called macrophages accumulate in solid tumors and are numerous in growing tumors of poor prognosis. Based on these results, many people consider that, for cancer patients, lymphocytes are good guys and macrophages are bad guys. However, the reality is likely to be not that Manichean. Indeed, immune responses that are efficient against infection require efficient cooperations between different cell types. We have designed a local treatment for tumor-bearing mice which has been conceived on the model of an anti-infectious response, as if there was an infection at the level of the tumor. This resulted in a systematic tumor regression.. We perform an accurate kinetic analysis of the immune response by combining immunofluorescence in tumor sections with a global analysis of the ...
Antigen-induced T cell arrest: the role of membrane tension | Single Molecule Science
Melanie Chabaud has been awarded a Long-Term Postdoctoral Fellowship from the Human Frontier Science Program (HFSP) to investigate the molecular events leading to T cell arrest.. T cells constantly migrate through the peripheral lymphoid organs, briefly pausing to sample the antigenic peptides presented by antigen presenting cells. Long-lasting interactions with antigen presenting cells, resulting in sustained signalling, is required to generate a robust immune response. But what makes them stop and what happens when they do?. Melanie believes that increased membrane tension in T cells triggers the critical events leading to T cell arrest, including reorganisation of the cytoskeleton and initiation of signalling cascades. Using purpose-built microchambers, she can observe T cells under the microscope as they recognise their target antigen and stop. She can then observe the actin cytoskeleton remodelling and other changes at the molecular level.. After completing her Ph.D. at the Institut Curie ...
SIV infection may increase T cells
Tissue in monkeys infected with a close relative of HIV can ramp up production of a type of T cell that actually weakens the bodys attack against the invading virus. The discovery, in lymph nodes draining the intestinal tract, could help explain how the HIV virus evades the bodys immune defenses. [en español]
HIV lifecycle | aidsmap
There are two different types of lymphocytes. B-lymphocytes (sometimes just called B-cells) produce antibodies. An antibody is a protein that can lock onto a distinctive part of a specific foreign organism. When this happens, the antibody signals to other immune cells to attack the organism.. T-lymphocytes (sometimes just called T-cells) are called different names depending on the molecules on their surface. CD4 cells (also known as CD4 T-lymphocytes, or T-helper cells) play a co-ordinating role in the immune system. They help B-lymphocytes identify foreign organisms (which they produce antibodies against). They also secrete substances that enable CD8 cells to reproduce. CD4 cells also activate macrophages (see below) to kill certain organisms, including many causes of AIDS-related illness. When CD4 cells are destroyed by HIV, all these parts of the immune system are disrupted. CD8 cells (also known as CD8 T-lymphocytes or cytotoxic T-cells) attach themselves to abnormal body cells, notably ...
HLA-A*02:01 PRAME142-151 Tetramer-SLYSFPEPEA-APC | Class I | MBL Life Science -JAPAN
HLA-A*02:01 PRAME142-151 Tetramer-SLYSFPEPEA-APC（Human Class I） from MBL.MHC tetramers can be used for direct detection of antigen specific T cells.
CD226 FITC | Caprico Biotechnologies
The clone 11A8, a mouse monoclonal antibody, recognizes a ~65 kDa cell surface glycoprotein known as CD226. It is a member of immunoglobulin superfamily containing two immunoglobulin-like variable domains. CD226 is widely expressed on peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and on a subset of B cells. It plays important role in the process of cytotoxic activities mediated by NK and T cells.
CWRU dental researcher demonstrates how T cells cause inflammation during infections | Positively Positive
PositivelyPositive.ca is designed to create awareness around the many HIV and AIDS issues and promotes messages of positive living with HIV
Fomina Laboratory | UC Davis Department of Physiology and Membrane Biology
T lymphocytes are cells of the immune system that produce factors regulating the function of other cells of immune system thereby shaping the systemic immune response. CD4+ T lymphocytes play a crucial role in directing the immune responses against foreign pathogens, damaged or transformed cells. However, when deregulated, the T cells may also mediate a number of autoimmune diseases such as type-1 diabetes, rheumatoid arthritis and multiple sclerosis. In addition, activated T cells mediate graft rejection and may significantly affect the development and progression of inflammatory diseases such as cardiovascular disease and stroke. Therefore, understanding the complex molecular mechanisms that regulate T cell effector functions may lead to development of therapeutic strategies designed to treat and/or alleviate T cell-mediated immune system disorders in humans. Our research focuses on unraveling the cellular and molecular mechanisms regulating T cell activation and communication with other cells ...
The PI3K p110delta controls T-cell development, differentiation and regulation
PI3Ks (phosphoinositide 3-kinases) regulate diverse cellular functions such as metabolism, growth, gene expression and migration. The p110delta isoform of PI3K is mainly expressed in cells of the immune system and contributes to cellular and humoral immunity. In the thymus, p110delta and p110gamma p …
Gene therapy to modify cultured T-lymphocytes - Stock Image G210/0538 - Science Photo Library
Gene therapy. A researcher prepares culture dishes of gene-corrected T-lymphocytes. T-lymphocytes are white blood cells that play a crucial role in the immune system. This stock of lymphocytes is derived from cells taken from a patient with a suppressed immune system. The original sample was genetically altered to make the cells more active, and then cultured to produce large numbers. The cells can then be reintroduced into the patient to help restore the immune system. - Stock Image G210/0538
Its Time To Stop Procrastinating, Because Entries For The B&T Awards Close Today! - B&T
The day weve been warning you about for weeks is here, and its time to get your ducks in a row. Entries for the B&T Awards will close today at 5pm!. If you havent started your entry yet, were not mad, were just disappointed. But you can remedy that by getting your entry in before 5pm today.. In case you hadnt caught on yet, thats the on-time entry deadline. Today, Friday August 3, 5pm. Winning one of these awards is no easy feat - youll need a killer entry to win. Which means you definitely should have started already. Forget any other work you have to do today and get started right here.. The B&T Awards are Australias biggest, longest-running and most entertaining awards for the advertising, marketing and media industries, and are judged by a panel of industry experts.. And all you have to do to win is enter! Which you must do by the end of today.. To repeat, entries close today at 5pm - and the entry fee is $399 + GST.. Here at B&T, were super understanding when happy hour at the pub ...
T-lymphocytes, SEM - Stock Image C023/9649 - Science Photo Library
Coloured scanning electron micrograph (SEM) of two T-lymphocyte white blood cells. Characteristic of T-lymphocytes are the long microvilli projecting from the cell surface. T-lymphocytes are susceptible to infection by the Human Immunodeficiency Virus (HIV), the causative agent of AIDS. Magnification 1300x at 35mm. - Stock Image C023/9649
CD3, PE-Cyanine5, clone: UCHT1, eBioscience™ 25 Tests; PE-Cyanine5 CD3, PE-Cyanine5, clone: UCHT1, eBioscience™
CD3, PE-Cyanine5, clone: UCHT1, eBioscience™ 25 Tests; PE-Cyanine5 CD3, PE-Cyanine5, clone: UCHT1, eBioscience™ Primary Antibodies CD1 to CD5
CD28 : CD80 interactions mediate antigen independent T cell adhesion and ring junction formation - Nuffield Department of...
The T Cell Marker, CD3 Antigen and Antibodies Mini-review | Bio-Rad
CD3 is an essential T cell co-receptorand defines T cell lineage. CD3is therefore an ideal T cell marker. This mini-review explains the structure of CD3, the genes involved in its expression, its function and the signal transduction pathways mediated by CD3 complex.
HEK293 vs HEK293T - Cell Biology
I was wondering if my transfections are not working because Im using the wrong cell line, I have been using HEK293, but is HEK293T better for transient and stable transfection? Also is there a difference with vendors (ie, some have better cell lines? lower passages perhaps?). Im just getting frustrated with these transfections that do not work. If anyone has any suggestions I would greatly appreciate it ...
What does t-lymphocytes mean?
Looking for the meaning of t-lymphocytes? Find out what is the meaning of t-lymphocytes on Phrases.net! The Webs largest and most authoritative phrases and idioms resource.
Major histocompatability complex | Article about Major histocompatability complex by The Free Dictionary
Looking for Major histocompatability complex? Find out information about Major histocompatability complex. In vertebrates, a family of genes that encode cell surface glycoproteins that regulate interactions among cells of the immune system, some components of the... Explanation of Major histocompatability complex
Primary human T lymphocytes engineered with a codon-optimized IL-15 gene resist cytokine withdrawal-induced apoptosis and...
TY - JOUR. T1 - Primary human T lymphocytes engineered with a codon-optimized IL-15 gene resist cytokine withdrawal-induced apoptosis and persist long-term in the absence of exogenous cytokine. AU - Hsu, Cary. AU - Hughes, Marybeth S.. AU - Zheng, Zhili. AU - Bray, Regina B.. AU - Rosenberg, Steven A.. AU - Morgan, Richard A.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2005/12/1. Y1 - 2005/12/1. N2 - IL-15 is a common γ-chain cytokine that has been shown to be more active than IL-2 in several murine cancer immunotherapy models. Although T lymphocytes do not produce IL-15, murine lymphocytes carrying an IL-15 transgene demonstrated superior antitumor activity in the immunotherapy of B16 melanoma. Thus, we sought to investigate the biological impact of constitutive IL-15 expression by human lymphocytes. In this report we describe the generation of a retroviral vector encoding a codon-optimized IL-15 gene. Alternate codon usage significantly enhanced the translational ...
T-cell hyporesponsiveness induced by activated macrophages through nitric oxide production in mice infected with Mycobacterium...
TY - JOUR. T1 - T-cell hyporesponsiveness induced by activated macrophages through nitric oxide production in mice infected with Mycobacterium tuberculosis. AU - Nabeshima, Shigeki. AU - Nomoto, Mari. AU - Matsuzaki, Goro. AU - Kishihara, Kenji. AU - Taniguchi, Hatsumi. AU - Yoshida, Shin Ichi. AU - Nomoto, Kikuo. PY - 1999. Y1 - 1999. N2 - In active tuberculosis, T-cell response to Mycobacterium tuberculosis is known to be reduced. In the course of Mycobacterium tuberculosis infection in mice, we observed that T-cell proliferation in response to M. tuberculosis purified protein derivative (PPD) reached the maximum level on day 7, then declined to the minimal level on day 14, and persisted at a low level through day 28 postinfection. The frequency of PPD-specific CD4 T cells in the spleen on day 28 decreased to one-sixth on day 7. To further investigate the mechanism of this T-cell hyporesponsiveness, we next analyzed the suppressive activity of spleen macrophages on T-cell function. The ...
The effect of dexamethasone, cyclosporine, and rapamycin on T-lymphocyte proliferation in vitro: Comparison of cells from...
TY - JOUR. T1 - The effect of dexamethasone, cyclosporine, and rapamycin on T-lymphocyte proliferation in vitro. T2 - Comparison of cells from patients with glucocorticoid-sensitive and glucocorticoid-resistant chronic asthma. AU - Haczku, Angela Franciska. AU - Alexander, Andrew. AU - Brown, Peter. AU - Assoufi, Basil. AU - Li, Baiqing. AU - Kay, A. Barry. AU - Corrigan, Christopher. PY - 1994. Y1 - 1994. N2 - Inhibition of T-lymphocyte activation may provide a useful approach to the treatment of chronic severe asthma. We compared rapamycin, a novel immunosuppressive drug, with cyclosporine and dexamethasone for its effects in inhibiting proliferation of T lymphocytes from patients with glucocorticoid-resistant and glucocorticoid-sensitive asthma. Phytohemagglutinin-stimulated peripheral blood T lymphocytes from 11 patients with clinically glucocorticoid-resistant and 8 patients with glucocorticoid-sensitive chronic asthma were tested for sensitivity to these drugs in a highly reproducible ...
Two adjacent residues in staphylococcal enterotoxins A and E determine T cell receptor V beta specificity. | Journal of...
The T cell receptor (TCR) V beta-determining region of two bacterial superantigens, staphylococcal enterotoxin A (SEA) and SEE, has been mapped to the COOH-terminal region of SEA and SEE using a panel of recombinant SEA/SEE hybrids. Total TCR V beta mRNA enrichment in human peripheral blood T cell cultures was determined by a novel single-tube amplification technique using a redundant V beta-specific primer. SEA routinely enriched mRNA coding for hV beta 1.1, 5.3, 6.3, 6.4, 6.9, 7.3, 7.4, and 9.1, while SEE, which is 83% homologous to SEA, enriched hV beta 5.1, 6.3, 6.4, 6.9, and 8.1 mRNA. Exchanging residues 206 and 207 was sufficient to convert in toto the TCR V beta response of human peripheral T lymphocytes. In addition, an SEA-reactive murine T cell line, SO3 (mV beta 17), unresponsive to wild-type SEE responded to SEE-S206N207, while an SEE-specific human T cell line, Jurkat (hV beta 8.1), unresponsive to SEA was stimulated strongly by SEA-P206D207. Exchanging all other regions of SEA and ...
Thalidomide Costimulates Primary Human T Lymphocytes, Preferentially Inducing Proliferation, Cytokine Production, and Cytotoxic...
We next examined the effect of thalidomide on cytokine production by the three T cell populations when stimulated by anti-CD3 in the absence of exogenous IL-2. Thalidomide induced a consistent concentration-dependent increase in IL-2 production at 12 h (time of peak production determined in preliminary kinetic experiments), in CD8+ and in bulk T cells (Fig. 3,A). Although a trend towards a thalidomide dose response in IL-2 production by purified CD4+ T cells was apparent, production of this cytokine was modest in comparison to that of bulk and CD8+ populations. Exposure to thalidomide consistently increased IFN-γ production by all three T cell populations. Since there was no clear peak in production of the latter cytokine, the results of a kinetic experiment are shown for CD4+ and CD8+ T cells (Fig. 3,B) using a single concentration of thalidomide (10 μg/ml). Again, the drug induced relatively greater production of IFN-γ by purified CD8+ T cells. However, the thalidomide-induced augmentation ...
Alloantigen-specific idiotype-bearing receptors on mouse t lymphocytes by B Rubin, wulff B. Hertel et al.
Rubin, B; Hertel, wulff B.; and Kimura, A, Alloantigen-specific idiotype-bearing receptors on mouse t lymphocytes. I. Specificity characterization and genetic association with the heavy-chain igg allotype. (1979). Subject Strain Bibliography 1979. 4576 ...
Hydrogen Peroxide Triggers a Dual Signaling Axis To Selectively Suppress Activated Human T Lymphocyte Migration.<...
TY - JOUR. T1 - Hydrogen Peroxide Triggers a Dual Signaling Axis To Selectively Suppress Activated Human T Lymphocyte Migration.. AU - Ball, Jennifer. AU - Vlisidou, Isabella. AU - Blunt, Matthew. AU - Wood, William. AU - Ward, Stephen. PY - 2017/5/1. Y1 - 2017/5/1. N2 - H2O2 is an early danger cue required for innate immune cell recruitment to wounds. To date, little is known about whether H2O2 is required for the migration of human adaptive immune cells to sites of inflammation. However, oxidative stress is known to impair T cell activity, induce actin stiffness, and inhibit cell polarization. In this study, we show that low oxidative concentrations of H2O2 also impede chemokinesis and chemotaxis of previously activated human T cells to CXCL11, but not CXCL10 or CXCL12. We show that this deficiency in migration is due to a reduction in inflammatory chemokine receptor CXCR3 surface expression and cellular activation of lipid phosphatase SHIP-1. We demonstrate that H2O2 acts through an Src ...
British Library EThOS: An investigation into the role of protein kinases in T lymphocyte migration
The migration of T lymphocytes is a vital component of the immune system, with roles in immunosurveillance and inflammation. The role of Phosphoinositide 3-kinase within T lymphocyte migration is unclear, with some evidence that it may be a disposable signal. Here, using Staphylococcal Enterotoxin B activated peripheral blood mononuclear cells and the T cell line CEM cells, the role of Phosphoinositide 3-kinase and its downstream kinases was investigated. CCL22 mediated CEM cell migration and CXCL12 mediated peripheral blood mononuclear cell migration were shown to be independent of Phosphoinositide 3-kinase using several different broad-spectrum Phosphoinositide 3-kinase inhibitors. However, these cells were Akt-dependent, as demonstrated by incubation with the Akt inhibitor Akti-1/2. Differences in the effect of the inhibitors on Akt activity were discovered, indicating that either Akt can be activated in the absence of Phosphoinositide 3-kinase, or differences exist regarding the relative ...
Malaria antigen screening method - Patent application
0065]Following incubation of the APCs with effector T lymphocytes obtained from a mammal immunized against the pathogen, the inventive method comprises screening for an immune response from the effector T lymphocytes. The immune response can be any suitable effector T lymphocyte immune response known in the art, including, but not limited to, cytokine secretion, effector T cell cytotoxicity, and immune activation of effector T cells. Preferably, the inventive method comprises screening for secretion by the effector T lymphocytes. In this regard, cytokine secretion from an effector T lymphocyte contacting an APC indicates that the effector T lymphocyte recognizes the antigen produced and displayed by the APC. Furthermore, it is well known in the art that effector T lymphocytes, such as effector helper T effector lymphocytes, secrete cytokines upon antigen recognition which promote different activities. In this regard, inflammatory or Th1 CD4 T cells produce interleukin-2 (IL-2), interferon gamma ...
Free Online Library: The Distribution of Activation Markers and Selectins on Peripheral T Lymphocytes in Preeclampsia.(Research Article, Report) by Mediators of Inflammation; Biological sciences B cells Analysis Disease susceptibility Physiological aspects Pregnant women T cells Health aspects Women Womens health
T-suppressor cell. Causes, symptoms, treatment T-suppressor cell
Description of disease T-suppressor cell. Treatment T-suppressor cell. Symptoms and causes T-suppressor cell Prophylaxis T-suppressor cell
In Vivo Suppression of HIV by Antigen Specific T Cells Derived from Engineered Hematopoietic Stem Cells
Author Summary There is a desperate need for the development of new therapeutic strategies to eradicate HIV infection. HIV actively subverts the potent natural immune responses against it, particularly cellular cytotoxic T lymphocyte (CTL) responses. The development of a therapy that allows long-lived immune self-containment of HIV and restoration of these CTL responses by the host would be ideal. Through genetic manipulation of human blood-forming stem cells, we introduced a molecule- an HIV-targeting T cell receptor (TCR)-that allowed the generation of functional HIV-specific CTLs following differentiation within human tissues in a humanized mouse model. To assess if these newly developed, HIV-specific CTLs can allow active suppression of HIV replication, we infected these mice with HIV. We found that the development of genetically modified, HIV-specific CTLs in these mice results in the presence of a functional antiviral CTL response in vivo that significantly lowers viral replication following HIV
Prevention of acute allograft rejection by antibody targeting of TIRC7, a novel T cell membrane protein. | Harvard Catalyst...
Utku N, Heinemann T, Tullius SG, Bulwin GC, Beinke S, Blumberg RS, Beato F, Randall J, Kojima R, Busconi L, Robertson ES, Schülein R, Volk HD, Milford EL, Gullans SR. Prevention of acute allograft rejection by antibody targeting of TIRC7, a novel T cell membrane protein. Immunity. 1998 Oct; 9(4):509-18 ...
Identification of apoB‐100 Peptide‐Specific CD8+ T Cells in Atherosclerosis | Journal of the American Heart Association
Our study shows that (1) CD8+ T‐cell activation and memory generation occurs in response to atherogenic diet feeding in apoE−/− mice; (2) A small fraction of these activated CD8+ T cells in apoE−/− mice are antigen‐specific that are reactive to the p210 peptide fragment of apoB‐100, supporting the notion that p210 is a self‐antigen; and (3) Immunization of apoE−/− mice with the p210 peptide fragment altered the immune‐dominant epitopes, concurrent with reduced atherosclerosis. This has significant implications in our efforts to characterize immune functions in atherosclerosis and develop potential apoB‐100 peptide‐based vaccine candidates.4, 22. T‐cell activation is a hallmark feature of adaptive immunity. Naïve T cells undergo activation and clonal expansion of antigen‐specific T cells when encountering antigen presented by antigen‐presenting cells in the context of costimulatory signaling. Such antigen‐specific T cells further differentiate into effector and ...
CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development. | JEM
CD27 is a lymphocyte-specific member of the TNF receptor family and has a TNF-related transmembrane ligand, CD70. The CD27/CD70 receptor-ligand pair cooperates with the TCR in the regulation of the peripheral T cell response. The study presented here reveals that CD27 may play a similar role in thymic pre-T cell development. We have previously cloned the cDNA encoding murine CD27, prepared specific mAbs and observed that murine CD27 is expressed on virtually all thymocytes, with the exception of a subpopulation of CD4-8- precursor T cells. It is shown here that induction of murine CD27 expression occurs at the transition from the CD4-8-25+ to the CD4-8-25- precursor T cell stage and is regulated by the pre-TCR. Therefore, we investigated whether CD27 contributes to pre-TCR-mediated thymocyte development. Pre-TCR function was mimicked by the induction of CD3 signaling in thymocytes of recombination activating gene (RAG)-deficient mice. This in vivo anti-CD3 epsilon mAb treatment induces an about ...
A Peptide-functionalized polymer as a minimal scaffold protein to enhance cluster formation in early T cell signal transduction
In cellular signal transduction, scaffold proteins provide binding sites to organize signaling proteins into supramolecular complexes and act as nodes in the signaling network. Furthermore, multivalent interactions between the scaffold and other signaling proteins contribute to the formation of protein microclusters. Such microclusters are prominent in early T cell signaling. Here, we explored the minimal structural requirement for a scaffold protein by coupling multiple copies of a proline-rich peptide corresponding to an interaction motif for the SH3 domain of the adaptor protein GADS to an N-(2-hydroxypropyl)methacrylamide polymer backbone. When added to GADS-containing cell lysates, these scaffolds (but not individual peptides) promoted the binding of GADS to peptide microarrays. This can be explained by the cross-linking of GADS into larger complexes. Furthermore, following import into Jurkat T cell leukemia cells, this synthetic scaffold enhanced the formation of microclusters of signaling ...
British Library EThOS: Genetic control of MTOR to improve adoptive T cell therapy of tumours
Adoptive T cell therapy to treat cancer in combination with re-directing specificity through T cell receptor (TCR) gene transfer, represents an effective therapeutic option. However, reduced effector responses due to the immunosuppressive tumour microenvironment and insufficient long-term engraftment of transferred cells represent two potential limitations. Tumours often employ mechanisms to inhibit T cell responses including secretion of TGFβ and depleting the tumour microenvironment of amino acids. The main aim of this PhD project was to develop a strategy to enhance T cell function for tumour therapy. The mammalian target of rapamycin (mTOR) pathway regulates CD8 T cell differentiation such that high mTOR activation leads to enhanced effector whilst low mTOR activation leads to increased T cell memory formation. Two retrovirus constructs have been designed whereby one expresses the positive mTOR regulator Rheb and the other expresses the negative mTOR regulator Pras40. Rheb transduction into ...
Restricted production of interleukin 4 by activated human T cells | PNAS
Interleukin 4 (IL-4) is secreted by activated T cells and pleiotropically modulates both B- and T-lymphocyte function. In murine helper (CD4+) T-cell clones IL-4 production appears to be regulated independently of interferon gamma and interleukin 2. To determine whether production of these lymphokines is also differentially regulated in uncloned human T cells, we studied lymphokine production by normal human peripheral T cells and T-cell subsets after in vitro polyclonal activation. After maximal induction of lymphokine expression, IL-4 mRNA was detectable in less than 5% of CD4+ and 1-2% of unfractionated T cells, whereas approximately 33% and 60% of CD4+ cells expressed detectable mRNA for interferon gamma and interleukin 2, respectively. This finding correlated with dramatically lower production of IL-4 mRNA and protein than of interferon gamma and interleukin 2 by peripheral blood and tonsillar T cells. The helper-inducer (CD4+ CD45R-) T-cell subset, which significantly enhances in vitro ...
AID 1270344 - Induction of cell proliferation in human Jurkat T cells at 200 uM after 24 hrs by tryphan blue assay - PubChem
BioAssay record AID 1270344 submitted by ChEMBL: Induction of cell proliferation in human Jurkat T cells at 200 uM after 24 hrs by tryphan blue assay.
Patterns of A2A Extracellular Adenosine Receptor Expression in Different Functional Subsets of Human Peripheral T Cells. Flow...
In this report we show that it is possible to detect the expression of a G protein-coupled receptor, the A2AR, in human lymphocytes both on the basis of a functional assay (cAMP accumulation) and by flow cytometry using an antireceptor mAb. We were able to describe for the first time the distribution of A2AR among minor T cell subpopulations through the use of a combination of anti-A2AR mAb and mAbs that recognize T cell surface markers or cytokines. The principle findings of this study are that much higher levels of A2AR expression is found in T cells than in B cells (Fig. 3) and higher levels of cytokines are detected in activated T cells that express A2AR than in activated T cells that do not express these receptors (Figs. 6 and 7).. The detection of higher levels of cytokines among A2AR+ cells is surprising because A2AR-mediated signaling antagonizes the effects of T cell activation (Koshiba et al., 1997; Huang et al., 1997). Therefore, we expected that cytokine secretion would be the lowest ...
Haloperidol (Haldol) - BET-bromodomain inhibition research
Background Different types of membrane microdomains (rafts) have been postulated to be present in the rear and front of polarized migrating T-lymphocytes. reorganization in human being T-lymphocytes and possible roles of flotillins in lymphocyte polarization. Results We studied flotillin reorganization and lateral mobility at the plasma membrane using immunofluorescence staining and FRAP (fluorescence recovery after photobleaching). We show that flotillins redistribute early upon chemokine stimulation and form very stable caps in the uropods of human peripheral blood T-lymphocytes colocalizing with the adhesion molecule PSGL-1 and activated ezrin/radixin/moesin (ERM) proteins. Chemokine-induced formation of stable flotillin caps requires Haloperidol (Haldol) integrity and dynamics of the actin cytoskeleton but is not abolished by inhibitors suppressing Rho-kinase or myosin II activity. Tagged flotillin-2 and flotillin-1 coexpressed in T-lymphocytes but Haloperidol (Haldol) not singly expressed ...
Tumor-derived exosomes regulate expression of immune function-related genes in human T cells | Exosome RNA
Tumor cell-derived exosomes (TEX) suppress functions of immune cells. Here, changes in the gene profiles of primary human T lymphocytes exposed in vitro to exosomes were evaluated. CD4(+) Tconv, CD8(+) T or CD4(+) CD39(+) Treg were isolated from normal donors peripheral blood and co-incubated with TEX or exosomes
The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent...
The human T cell receptor for antigen (Ti) has recently been identified on IL-2 dependent T cell clones as a 90 kd disulfide-linked heterodimer comprised of one 49-51 kd alpha (alpha) and one 43 kd beta (beta) chain. These subunits are noncovalently associated with a monomorphic 20-25 kd T3 molecule. Here, we produce monoclonal antibodies to a human tumor (REX) derived from an earlier stage of thymic differentiation in order to determine whether clonotypic structures are expressed and to define the ontogeny of Ti. The results of SDS-PAGE and peptide map analyses indicate that an homologous T3-associated heterodimer is synthesized and expressed by REX. This glycoprotein shares several peptides in common with clonotypic structures on an IL-2 dependent T cell clone. In addition, similar Ti related molecules appear during intrathymic ontogeny in parallel with surface T3 expression. The latter findings provide the structural basis for the immunological competence observed exclusively within the T3+ thymocyte
The human T cell receptor: appearance in ontogeny and biochemical relationship of alpha and beta subunits on IL-2 dependent...
The human T cell receptor for antigen (Ti) has recently been identified on IL-2 dependent T cell clones as a 90 kd disulfide-linked heterodimer comprised of one 49-51 kd alpha (alpha) and one 43 kd beta (beta) chain. These subunits are noncovalently associated with a monomorphic 20-25 kd T3 molecule. Here, we produce monoclonal antibodies to a human tumor (REX) derived from an earlier stage of thymic differentiation in order to determine whether clonotypic structures are expressed and to define the ontogeny of Ti. The results of SDS-PAGE and peptide map analyses indicate that an homologous T3-associated heterodimer is synthesized and expressed by REX. This glycoprotein shares several peptides in common with clonotypic structures on an IL-2 dependent T cell clone. In addition, similar Ti related molecules appear during intrathymic ontogeny in parallel with surface T3 expression. The latter findings provide the structural basis for the immunological competence observed exclusively within the T3+ thymocyte
Virus-Specific T-Cell Sensitization. Requirements for vaccinia virus specific T cell sensitization in vivo.
Ertl, Hildegund; Gerike, Rainer und Koszinowski, Ulrich H. (1977): Virus-Specific T-Cell Sensitization. Requirements for vaccinia virus specific T cell sensitization in vivo. In: Journal of immunogenetics, Vol. 4: S. 515-522 ...
4-1BB Ligand | CTD
A membrane bound member of the TNF superfamily that is expressed on activated B-LYMPHOCYTES; MACROPHAGES; and DENDRITIC CELLS. The ligand is specific for the 4-1BB RECEPTOR and may play a role in inducing the proliferation of activated peripheral blood T-LYMPHOCYTES ...
Increased antitumor efficacy of PD-1- deficient melanoma-specific human lymphocytes - Inserm
BACKGROUND: Genome editing offers unique perspectives for optimizing the functional properties of T cells for adoptive cell transfer purposes. So far, PDCD1 editing has been successfully tested mainly in chimeric antigen receptor T (CAR-T) cells and human primary T cells. Nonetheless, for patients with solid tumors, the adoptive transfer of effector memory T cells specific for tumor antigens remains a relevant option, and the use of high avidity T cells deficient for programmed cell death-1 (PD-1) expression is susceptible to improve the therapeutic benefit of these treatments. METHODS: Here we used the transfection of CAS9/sgRNA ribonucleoproteic complexes to edit PDCD1 gene in human effector memory CD8+ T cells specific for the melanoma antigen Melan-A. We cloned edited T cell populations and validated PDCD1 editing through sequencing and cytometry in each T cell clone, together with T-cell receptor (TCR) chains sequencing. We also performed whole transcriptomic analyses on wild-type (WT) and
sequence alignment Protocols and Video...
Video articles in JoVE about sequence alignment include Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates, Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group, A Practical Guide to Phylogenetics for Nonexperts, Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin, Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web, Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules, CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion, Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq, Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency, Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution, Optimization of Synthetic Proteins:
Redirecting therapeutic T cells against myelin-specific T lymphocytes using a humanized myelin basic protein-HLA-DR2-zeta...
Therapies that Ag-specifically target pathologic T lymphocytes responsible for multiple sclerosis (MS) and other autoimmune diseases would be expected to have improved therapeutic indices compared with Ag-nonspecific therapies. We have developed a cellular immunotherapy that uses chimeric receptors to selectively redirect therapeutic T cells against myelin basic protein (MBP)-specific T lymphocytes implicated in MS. We generated two heterodimeric receptors that genetically link the human MBP84-102 epitope to HLA-DR2 and either incorporate or lack a TCRzeta signaling domain. The Ag-MHC domain serves as a bait, binding the TCR of MBP-specific target cells. The zeta signaling region stimulates the therapeutic cell after cognate T cell engagement. Both receptors were well expressed on primary T cells or T hybridomas using a tricistronic (alpha, beta, green fluorescent protein) retroviral expression system. MBP-DR2-zeta-, but not MBP-DR2, modified CTL were specifically stimulated by cognate MBP-specific T
At first glance, an association of a CD4 T cell-mediated disease with HLA class II gene products, whose function is to present peptides to CD4 T cells, appears easily explainable. Over the years, the most obvious, nonexclusive theories have been tested: instability and poor peptide binding of diabetogenic HLA class II molecules (6), unique peptide repertoire of the same molecules (7), T cells focused on the recognition of HLA-DQβ57 (8), failed thymic selection of autoreactive T cells (9), and abnormal T-cell binding to autoimmune peptide-MHC complexes (10). While all of those might bear truth and give some level of understanding of what the β57 residue might do, none could formally associate the mutation to a molecular mechanism leading to diabetes. The closest one to explaining the association of the same mutation with a disease was in the context of celiac disease, where the same HLA-DQ molecules are strongly predisposing to onset and also promote a frequent association with type 1 diabetes ...
Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities<...
TY - JOUR. T1 - Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities. AU - Parolini, Ornella. AU - Magatti, Marta. AU - De Munari, Silvia. AU - Vertua, Elsa. AU - Gibelli, Lucia. AU - Wengler, Georg S.. PY - 2008. Y1 - 2008. N2 - Cells derived from the amniotic membrane of human placenta have been receiving particular attention because of their stem cell potentiality and immunomodulatory properties, which make them an attractive candidate source for cell therapy approaches. In this study, we isolated cells from the mesenchymal region of amnion and identified two subpopulations discordant for expression of the HLA-DR, CD45, CD14, and CD86 cellular markers. We therefore refer to the unfractionated cell population derived from this region as amniotic mesenchymal tissue cells (AMTC). We studied the suppressive and stimulatory characteristics of the unfractionated, HLA-DR-positive, and HLA-DR-negative AMTC populations and demonstrated that all three ...
Mechanisms of phosphatidylserine exposure, a phagocyte recognition signal, on apoptotic T lymphocytes
The appearance of phosphatidylserine (PS) on the cell surface during apoptosis in thymocytes and cytotoxic T lymphocyte cell lines provokes PS-dependent recognition by activated macrophages. Flow cytometric analysis of transbilayer lipid movements in T lymphocytes undergoing apoptosis reveals that d …
Multiple sclerosis: Newly discovered signal mechanism causes T cells to turn pathogenic | EurekAlert! Science News
T cells are an important part of the immune system. However, they are not only capable of destroying pathogens. They can also become harmful themselves. Researchers at the Technical University of Munich and the University Medical Center of the Johannes Gutenberg University Mainz have discovered the conditions under which certain T cells become the kind of pathogenic T cells associated with multiple sclerosis. Their results explain why certain treatments are not consistently effective.
Since TNFα is constitutively synthesized in the thymus, we explored its role during human early T-cell development. TNFα accelerated early T-cell differentiation and greatly increased the number of CD34-CD7+CD5- T-cell precursors generated in the in vitro DL-4 culture both for cord blood and adult CD34+ HSPCs. TNFα improved the production of CD34-CD7+CD5-/lo cells at the expense of myeloid and NK progenitors leading to a highly purified population of CD7+ T cell precursors. These T-cell precursors expressed early T-cell commitment markers as shown by extensive RNAseq analysis and had a high T-cell differentiation potential in vitro upon differentiation on OP9/DL1 cells and in vivo when transplanted to NOD/SCID/γc-/- mice. TNFα increased specifically the rate of proliferation in CD7+ T cell precursors between day 4 and 7 by promoting T-cell progenitors entry into the cell cycle through the activation of NFkB pathway. ...
Mechanisms of Cellular Avidity Regulation in CD2-CD58-Mediated T Cell Adhesion. - The Kennedy Institute of Rheumatology
The CD2 receptor on T lymphocytes is essential for T cell adhesion and stimulation by antigen presenting cells (APCs). Blockade of CD2 function is immunosuppressive in both model systems and humans, indicating the importance of CD2 for the cellular immune response. Although the affinity of the molecular interaction between CD2 and its counter-receptor, CD58, is relatively low when measured in solution, this interaction mediates tight adhesion within the 2D cell-cell interface. To understand the mechanisms responsible for regulating the avidity of the CD2-CD58 interaction, we measured the number, affinity, and lateral mobility of CD2 molecules on resting and activated T cells. Cell activation caused a 1.5-fold increase in the number of CD2 sites on the cell surface, and the 2D affinity of CD2 for CD58 increased by 2.5-fold. The combination of T cell activation and CD2 ligation to CD58 decreased the laterally mobile fraction of the ligated CD2. Together, these changes would substantially enhance CD2
The rapid isolation of clonable antigen-specific T lymphocyte lines capable of mediating autoimmune encephalomyelitis. |...
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Intersection of population variation and autoimmunity genetics in human T cell activation « Research Papers « Institute for...
by Barbara Stranger, Ye CJ, Feng T, Kwon HK, Raj T, Wilson MT, Asinovski N, McCabe C, Lee MH, Frohlich I, Paik HI, Zaitlen N, Hacohen N, De Jager P, Mathis D, Regev A, Benoist C. T lymphocyte activation by antigen conditions adaptive immune responses and immunopathologies, but we know little about its variation in humans and its genetic or environmental roots. We analyzed gene expression in CD4(+) T cells during unbiased activation or in T helper 17 (T(H)17) conditions from 348 healthy participants representing European, Asian, and African ancestries. We observed interindividual variability, most marked for cytokine transcripts, with clear biases on the basis of ancestry, and following patterns more complex than simple T(H)1/2/17 partitions. We identified 39 genetic loci specifically associated in cis with activated gene expression. We further fine-mapped and validated a single-base variant that modulates YY1 binding and the activity of an enhancer element controlling the autoimmune-associated ...
Specific regulation of Fos family transcription factors in thymocytes at two developmental checkpoints - CaltechAUTHORS
A central question in T cell development is what makes cortical thymocytes respond to stimulation in a qualitatively different way than any other thymocyte subset. Part of the answer is that AP-1 function changes drastically at two stages of T cell development. It undergoes striking down-regulation as thymocytes differentiate from immature, CD4^-CD8^- double-negative (DN) TCR^- thymocytes to CD4^+CD8^+ double-positive (DP) TCR^(lo) cortical cells, and then returns in the cells that mature to TCR^(high), CD4^+CD8^- or CD4^-CD8^+ single-positive (SP) thymocytes. At all three stages, the jun family mRNAs can be induced similarly. However, we demonstrate that DP cortical thymocytes are specifically impaired in c-fos and fosB mRNA induction, even when stimuli are used that optimize survival of the cells and a form of in vitro maturation. fra-2 expression is induction independent but much lower in DP cells than in the other subsets. Overall Fos family protein induction accordingly is severely ...
LY294002 and rapamycin co-operate to inhibit T-cell proliferation -ORCA
1. T-cell proliferation is critical for mounting an effective adaptive immune response. It is regulated by signals through the T-cell receptor, through co-stimulation and through cytokines such as interleukin-2 (IL-2). Phosphatidylinositol 3-kinase (PI3K) lies downstream of each of these pathways and has been directly implicated in the regulation of lymphocyte proliferation. 2. In this study, we have shown that PI3K regulates cyclin D2 and cyclin D3, the first cell cycle proteins induced in T-cell proliferation, transcriptionally and post-transcriptionally. In T-lymphoblasts, LY294002, a PI3K inhibitor, prevents the induction of both D-type cyclin mRNA and protein, while rapamycin inhibits the induction of protein. Rapamycin inhibits mammalian target of rapamycin (mTOR), which lies downstream of PI3K. 3. Furthermore, our data show that the combination of LY294002 and rapamycin results in a co-operative inhibition of T-cell proliferation. This co-operation occurs in Kit225 cells stimulated with ...
Antigen-stimulated apoptotic T-cell death in HIV infection is selective for CD4+ T cells, modulated by cytokines and effected...
TY - JOUR. T1 - Antigen-stimulated apoptotic T-cell death in HIV infection is selective for CD4+ T cells, modulated by cytokines and effected by lymphotoxin. AU - Clerici, Mario. AU - Sarin, Apurva. AU - Berzofsky, Jay A.. AU - Landay, Alan L.. AU - Kessler, Harold A.. AU - Hashemi, Farah. AU - Hendrix, Craig W.. AU - Blatt, Stephen P.. AU - Rusnak, Janice. AU - Dolan, Matthew J.. AU - Coffman, Robert L.. AU - Henkart, Pierre A.. AU - Shearer, Gene M.. PY - 1996. Y1 - 1996. N2 - Objective: To characterize the mechanism of in vitro antigen-induced apoptotic T-cell death in the peripheral blood mononuclear cells (PBMC) of HIV-1-infected individuals. Design and methods: PBMC from HIV-1-infected and uninfected individuals were unstimulated or stimulated with HIV-1 envelope synthetic peptides (Env) or influenza A virus to determine the extent of antigen-stimulated apoptotic T-cell death, whether this death was limited to the CD4+ subset, and the effects of cytokines on T-cell death. Death was ...
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This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown ...
Real-time Detection and Modulation of Human T Cell Activation Using Agilent Seahorse XF Hu T Cell Activation Assay Kit
Download this application note to discover a turnkey solution for studying T cell activation and associated metabolic reprogramming.
Imaging T-lymphocytes in inflammatory diseases: a nuclear medicine approach - The Quarterly Journal of Nuclear Medicine and...
Increasing interest and research efforts have been made in search of specific radiolabelled probes for imaging different immune cells (including T-lymphocytes) in inflammation and infection. This has led to early detection of lymphocyte infiltration, and the deepening of our understanding of pathogenesis of immune mediated diseases. In-vivo imaging of T-lymphocytes with radiolabelled specific probes may provide an important piece of information about inflammatory lesions, which could be very important to understand the molecular mechanism of action of any drug and/or their effect on the microenvironment of the immune system of the body. The present review focuses on radiolabelled T-lymphocytes and different monoclonal antibodies, peptides, cytokines, chemokine used for scintigraphic imaging of T-lymphocytes and their subsets.. ...
White blood cell
Lymphocyte. Main article: Lymphocyte. Lymphocytes are much more common in the lymphatic system than in blood. Lymphocytes are ... Lymphocyte. 30%. Small lymphocytes 7-8. Large lymphocytes 12-15. *B cells: releases antibodies and assists activation of T ... lymphocytes) by hematopoietic lineage (cellular differentiation lineage). Lymphocytes can be further classified as T cells, ... Lymphocytes include: *B cells make antibodies that can bind to pathogens, block pathogen invasion, activate the complement ...
T independent antigen (TI)
T independent antigen elicits antibody production by B lymphocytes without T lymphocyte involvement. There are 2 distinct ... Antibody production independent of T lymphocytes. For most protein antigens, the production of antibodies by B ... But when the concentration of TI-1 is lower, it can activate only B lymphocytes with specific binding of TI-1 on their BCR, and ... TI-1 antigens activate B-cells via Toll like receptors, which are, in human, expressed on the surface of B lymphocytes after ...
Transformation of B-lymphocytes. When EBV infects B cells in vitro, lymphoblastoid cell lines eventually emerge that are ... and I infections in B-lymphocytes. EBV latent infection of B-lymphocytes is necessary for virus persistence, subsequent ... EBV Latency III and II infections of B-lymphocytes, Latency II infection of oral epithelial cells, and Latency II infection of ... The latent programs reprogram and subvert infected B-lymphocytes to proliferate and bring infected cells to the sites at which ...
A lymphoblast is a modified naive lymphocyte with altered cell morphology. It occurs when the lymphocyte is activated by an ... Lymphoblasts can also refer to immature cells which typically differentiate to form mature lymphocytes. Normally ... Lymphoblasts look like immature lymphocytes, and were once thought to be precursor cells.". Commonly, when speaking about ... The Chronic Lymphocytic Leukemia Research Consortium defines a lymphoblast as "A lymphocyte that has become larger after being ...
Epidermal growth factor
Lymphocytes Stimulate growth and maturation of T-cells Mechanism. Diagram showing key components of the MAPK/ERK pathway ...
Natural killer cell
... granular lymphocytes known today as NK cells. The demonstration that density gradient-isolated large granular lymphocytes were ... Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. The role NK cells ... A functional marker of human non-T lymphocytes". Clinical and Experimental Immunology. 21 (2): 226-35. PMC 1538269. PMID 810282 ... Large granular lymphocyte entry in the public domain NCI Dictionary of. *. Calmeiro J, Carrascal M, Gomes C, Falcão A, Cruz MT ...
T helper cell
These effects are primarily due to the loss of any helper T cell that can interact with the B lymphocyte correctly. Another ... Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (1999). "Two subsets of memory T lymphocytes with distinct homing ... commonly lymphocytes and macrophages, resulting in chronic inflammation and cytokine release. Antibodies do not play a direct ... "Molecular interactions mediating T-B lymphocyte collaboration in human lymphoid follicles. Roles of T cell-B-cell-activating ...
A T cell, or T lymphocyte, is a type of lymphocyte (a subtype of white blood cell) that plays a central role in cell-mediated ... within a population of lymphocytes known as intraepithelial lymphocytes. In rabbits, sheep, and chickens, the number of γδ T ... T cells can be distinguished from other lymphocytes, such as B cells and natural killer cells, by the presence of a T-cell ... The T lymphocyte activation pathway: T cells contribute to immune defenses in two major ways; some direct and regulate immune ...
Once Upon a Time... Life
The Lymphocytes - represented by: *Lymphocytes B as marshals in small one-man round flying craft with two aimable side-mounted ... Lymphocytes T: the same sort of craft but with a large uppercase T on the underbelly at the bow. They can discharge smoke that ... Immature leucocytes: teenage humanoids with the same uniform as the lymphocyte B pilots: seen in the bone marrow, which is ... Captain Courageous and Ace for the lymphocyte B crafts' pilots; Plasmus and Globina for Hemo and Globin, Corpo for Jumbo; ...
Intraepithelial lymphocytes: distributed throughout the tissue.. *Intraepithelial macrophages. Stereocilia. The ...
T-lymphocytes. Glycoproteins. Budding. Nucleus. Nucleus. Nasal and ocular secretion Percavirus. Mammals. B-lymphocytes. ... B-lymphocytes. Glycoproteins. Budding. Nucleus. Nucleus. Saliva Rhadinovirus. Humans; mammals. B-lymphocytes. Glycoproteins. ... The gammaherpesviruses, including HVS, EBV, KSHV, and RRV, are capable of establishing latent infection in lymphocytes. ... A striking feature of the four gammaherpesviruses is that they contain distinct ORFs involved in lymphocyte signaling events. ...
Protein arginine methyltransferase 5
Peripheral blood lymphocytes. Normal Micronucleus test. Normal Heart weight. Normal Brain histopathology. Normal ...
Peripheral blood lymphocytes. Normal Micronucleus test. Normal Heart weight. Normal Skin Histopathology. Normal ...
Peripheral blood lymphocytes. Normal Micronucleus test. Normal Heart weight. Normal Skin Histopathology. Normal ...
Peripheral blood lymphocytes. Normal. Micronucleus test. Normal. Heart weight. Normal. Brain histopathology. Normal. ...
Peripheral blood lymphocytes. Normal Heart weight. Normal Salmonella infection. Normal Citrobacter infection. Normal ...
The mantle zone (or just mantle) of a lymphatic nodule (or lymphatic follicle) is an outer ring of small lymphocytes ...
Polyclonal B cell response
After recognizing an antigen, an antigen presenting cell such as the macrophage or B lymphocyte engulfs it completely by a ... or B lymphocytes) involving an arm of the immune system known as humoral immunity. The antibodies are soluble and do not ... The role of lymphocytes in mediating both cell-mediated and humoral responses was demonstrated by James Gowans in 1959. ... What makes the analogy even stronger is that the B lymphocytes have to compete with each other for signals that promote their ...
Suppresses the action of lymphocytes. *Possesses lytic enzymes, cytolysin, aggregation substance, pheromones, and lipoteichoic ...
Lymphocyte-rich. Is a rare subtype, show many features which may cause diagnostic confusion with nodular lymphocyte predominant ... Lymphocyte depleted. Is a rare subtype, composed of large numbers of often pleomorphic RS cells with only few reactive ... Nodular lymphocyte predominant Hodgkin's lymphoma expresses CD20, and is not currently considered a form of classical Hodgkin's ... Micrograph showing a "popcorn cell", the Reed-Sternberg cell variant seen in nodular lymphocyte predominant Hodgkin lymphoma. H ...
Systemic lupus erythematosus
Necrosis is increased in T lymphocytes.. Tingible body macrophages (TBMs) - large phagocytic cells in the germinal centers of ... B and T cell tolerance for apoptotic cells is abrogated, and the lymphocytes get activated by these autoantigens; inflammation ... The cytokines B-lymphocyte stimulator (BLys), interleukin 6, interleukin 17, interleukin 18, type I interferons, and tumor ... The body's sensitized B-lymphocyte cells will now produce antibodies against these nuclear-related proteins. These antibodies ...
Disease-modifying antirheumatic drug
Reducing numbers of T-lymphocytes etc.. unknown. etanercept. decoy TNF receptor. bDMARD. ...
Association with p56lck in T lymphocytes". The Journal of Biological Chemistry. 271 (5): 2863-7. doi:10.1074/jbc.271.5.2863. ... CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, ... "Lymphocyte CD44 binds the COOH-terminal heparin-binding domain of fibronectin". The Journal of Cell Biology. 116 (3): 817-25. ... protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes". ...
White blood cell - Simple English Wikipedia, the free encyclopedia
Lymphocytes[change , change source]. Lymphocytes are round white blood cells a bit bigger than a red blood cell. Their center ... There are three known types of lymphocytes, called T-cells, B-cells, and natural killer cells (NK cells). ...
The B lymphocyte, in this ready-to-respond form, is known as a "naive B lymphocyte." The naive B lymphocyte expresses both ... Nemazee D (2006). "Receptor editing in lymphocyte development and central tolerance". Nat Rev Immunol. 6 (10): 728-740. doi: ... How Lymphocytes Produce Antibody from Cells Alive!. *Antibody applications Fluorescent antibody image library, University of ... Goding J (1978). "Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation". Contemp Top ...
In the lymphatic system a lymph node is a secondary lymphoid organ. Lymph nodes contain lymphocytes, a type of white blood ... In order to do this, lymph nodes contain lymphocytes, a type of white blood cell, which includes B cells and T cells. These ... Both B and T lymphocytes enter lymph nodes from circulating blood through specialized high endothelial venules found in the ... In the course of the lymph, lymphocytes may be activated as part of the adaptive immune response. ...
Allergic contact dermatitis
In a similar fashion, cytotoxic T lymphocytes patrol an area of skin and play an important role in controlling both the ... Their immunology centres on the interaction of immunoregulatory cytokines and discrete subpopulations of T lymphocytes. The ... and present the antigen to T-lymphocytes. This process is controlled by cytokines and chemokines - with tumor necrosis factor ... the differentiated DCs present the allergenic epitope associated with the allergen to T lymphocytes. These T cells then divide ...
The thymic cortex, mainly composed of lymphocytes; functions as a site for somatic recombination of T cell receptors, and ...
Associated organs composed of lymphoid tissue are the sites of lymphocyte production. Lymphocytes are concentrated in the lymph ... the spleen retains the ability to produce lymphocytes. The spleen stores red blood cells and lymphocytes. It can store enough ... The tertiary lymphoid tissue[clarification needed] typically contains far fewer lymphocytes, and assumes an immune role only ... The thymus is a primary lymphoid organ and the site of maturation for T cells, the lymphocytes of the adaptive immune system. ...
"CD8 alpha - Marker for cytotoxic T Lymphocytes". Archived from the original on 21 September 2015. Retrieved 11 January 2016.. ... The cytoplasmic tails of the CD8 co-receptor interact with Lck (lymphocyte-specific protein tyrosine kinase). Once the T cell ... "CD8 alpha - Marker for cytotoxic T lymphocytes". Archived from the original on 2015-09-21.. ...
Frontiers | Unperturbed Cytotoxic Lymphocyte Phenotype and Function in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome...
... adrenaline and cytotoxic lymphocyte function, we re-assessed the role of cytotoxic lymphocytes in ME/CFS. Forty-eight patients ... adrenaline and cytotoxic lymphocyte function, we re-assessed the role of cytotoxic lymphocytes in ME/CFS. Forty-eight patients ... cytotoxicity lymphocyte aberrances were not found among ME/CFS patients. These assessments of cytotoxic lymphocytes therefore ... Cytotoxic lymphocytes combat intracellular infections. Their function is attenuated by stress. Despite numerous studies, the ...
Lymphocyte - Wikipedia
A lymphocyte is one of the subtypes of a white blood cell in a vertebrates immune system. Lymphocytes include natural killer ... Tumor-infiltrating lymphocytes. Main article: Tumor-infiltrating lymphocyte. In some cancers, such as melanoma and ... A lymphocyte count is usually part of a peripheral complete blood cell count and is expressed as the percentage of lymphocytes ... The formation of lymphocytes is known as lymphopoiesis. B cells mature into B lymphocytes in the bursa equivalent, which in ...
Category:Lymphocytes - Wikimedia Commons
Lymphocyte, Lymphocytes, سیالویاتی, Lymphocytic (ur); Limfocit (hr); лімфоцит (uk); Lymfocyter (sv); Limfocyty (pl); לימפוציטים ... Dosieroj en kategorio "Lymphocytes". La jenaj 77 dosieroj estas en ĉi tiu kategorio, el 77 entute. ... lymphocyte (en); خلية لمفاوية (ar); 淋巴細胞 (yue); Лимфоциттер (ky); Lìm-pâ Se-pâu (hak); Lymphozyt (nds); Lymphozyt (de); ... lymphocyte (fr); Limfosit (jv); Limfociti (hr); ލިމްފަސައިޓް (dv); Bạch huyết bào (vi); لىيمفوتسىيتتەر (kk-arab); Lïmfocïtter ( ...
Lymphocytes: Levels, ranges, and functions
Learn more about lymphocytes, a type of white blood cell. We look at their function, normal levels, and what happens if levels ... There are two categories of lymphocytes known as B lymphocytes and T lymphocytes. These are commonly referred to as B cells and ... The normal lymphocyte range in adults is between 1,000 and 4,800 lymphocytes in 1 microliter (µL) of blood. In children, the ... In children, the lymphocyte count would be around 9,000 lymphocytes in 1 µL of blood, although this value can change with age. ...
Anti-lymphocyte globulin - Wikipedia
T Cells or T-Lymphocytes | Encyclopedia.com
Source for information on T Cells or T-Lymphocytes: World of Microbiology and Immunology dictionary. ... T cells or T-lymphocytes When a vertebrate encounters substances that are capable of causing it harm, a protective system known ... The T-8 lymphocytes differentiate into cytotoxic T-lymphocytes (CTLs) that can destroy the body cells that have the original ... T Cells or T-Lymphocytes. Updated About encyclopedia.com content Print Article Share Article ...
Immunology of tumor infiltrating lymphocytes. - PubMed - NCBI
GIMAP Proteins in T-Lymphocytes
T. Nitta and Y. Takahama, "The lymphocyte guard-IANs: regulation of lymphocyte survival by IAN/GIMAP family proteins," Trends ... S. J. McLeod and M. R. Gold, "Activation and function of the Rap1 GTPase in B lymphocytes," International Reviews of Immunology ... E. Genot and D. A. Cantrell, "Ras regulation and function in lymphocytes," Current Opinion in Immunology, vol. 12, no. 3, pp. ... P. Poussier, A. F. Nakhooda, J. A. Falk, C. Lee, and E. B. Marliss, "Lymphopenia and abnormal lymphocyte subsets in the "BB" ...
Lymphocyte homing. - PubMed - NCBI
What causes elevated lymphocytes? | Reference.com
... or the presence of a high number of lymphocytes, can be caused by many different disorders and diseases, including ... What is the cause of having a high lymphocyte count in the blood?. A: A high lymphocyte count is referred to as lymphocytosis ... The Mayo Clinic asserts that the exact thresholds at which a high number of lymphocytes in a blood sample is considered to ... Children have much higher lymphocyte counts than adults, and the count tends to vary with age. In some cases, children must ...
lymphocytes lower than 20, Reference.com
Lymphocyte Count - an overview , ScienceDirect Topics www.sciencedirect.com/topics/immunology-and-microbiology/lymphocyte-count ... www.sightdx.com/knowledge-center/lymphocyte. Everything you need to know about Lymphocytes: definition count and normal ranges ... Jul 12, 2019 ... Lymphocytes help fight off diseases, so its normal to see a temporary increase after an infection. A count ... Lymphocyte counts are low and cellular responses to mitogens and antigens usually become ... A significant association between ...
Intraepithelial lymphocytes: exploring the Third Way in immunology | Nature Immunology
Locally resident intraepithelial lymphocytes (IELs) are primarily T cells with potent cytolytic and immunoregulatory capacities ... Regulatory function for murine intraepithelial lymphocytes. Two subsets of CD3+, T cell receptor-1+ intraepithelial lymphocyte ... Intraepithelial lymphocytes traffic to the intestine and enhance resistance to Toxoplasma gondii oral infection. J. Immunol. ... Novel function for intestinal intraepithelial lymphocytes: murine CD3+, γ/δ TCR+ T cells produce IFN-γ and IL-5. J. Immunol. ...
RhymeZone: lymphocyte definitions
CiNii Articles - B-Lymphocytes
T and B lymphocytes flow out from the arterial terminal, and migrate in the reticular framework. … Homing of lymphocytes to ... CD3+ and Pax5+ Lymphocytes in the Dermis of Normal Skin from the Dorsolateral Thorax of Cats [in Japanese] Fickle Daniel C. , ... Decreased Expression of MicroRNA-107 in B Lymphocytes of Patients with Antibody-Mediated Renal Allograft Rejection Zhang Zhe- ... mAb BW-3C3 react to a fraction of the lymphocytes. … The mAb-positive cells were identical to cells that also stained with ...
Voltage-Sensitive Ion Channels in Human B Lymphocytes | SpringerLink
In the immune system such diverse cell types as T lymphocytes, B lymphocytes, macrophages, neutrophils, hybridomas, and natural ... In: Gupta S., Paul W.E. (eds) Mechanisms of Lymphocyte Activation and Immune Regulation II. Springer, Boston, MA. * DOI https ... Subset-specific expression of potassium channels in developing murine T lymphocytes. Science 239: 771 (1988).PubMedCrossRef ... Cahalan MD, Chandy KG, DeCoursey TE, Gupta S, Lewis R, Sutro JB: Ion channels in T lymphocytes. In: Gupta S, Paul WE, and Fauci ...
Counting Lymphocytes in Histopathology Images Using Connected Components | SpringerLink
... a method for automatic counting of lymphocytes in histopathology images using connected components is presented. Our multi-step ... In this paper, a method for automatic counting of lymphocytes in histopathology images using connected components is presented ... Graf F., Grzegorzek M., Paulus D. (2010) Counting Lymphocytes in Histopathology Images Using Connected Components. In: Ünay D ... Application to lymphocyte segmentation on breast cancer histopathology. IEEE Transactions on Biomedical Engineering 57(7), 1676 ...
JCI - Reparative T lymphocytes in organ injury
High WBC, Low lymphocytes - Leukemia & Lymphoma - MedHelp
Lymphocyte 3.7 (L) Lymphocyte Absolute 0.6 (L) Absolute Neutrophil 14.9 (H) He has always been a super healthy kid. He seems to ... Lymphocyte 3.7 (L) Lymphocyte Absolute 0.6 (L) Absolute Neutrophil 14.9 (H) He has always been a super healthy kid. He seems to ... High WBC, Low lymphocytes. My 12yr old son had some blood work done in the ER, we were there due to some severe abdominal pain ... lymphocyte 0.25 - 0.33 mine is 0.25 my rbc is low what does this mean?? ive been diagnose of ptb ...
Colchicine inhibits phosphatidylinositol turnover induced in lymphocytes by concavalin A | Nature
... turnover in lymphocytes since it is known that increased PI turnover is an early event induced by con A and other mitogenic ... colchicine also has been shown to inhibit con A-induced lymphocyte transformation at an early stage in the sequence of events ... con A inhibition of lymphocyte immunoglobulin receptor capping is reversed by colchicine2-4 and con A-induced aggregation of ... Colchicine inhibits phosphatidylinositol turnover induced in lymphocytes by concavalin A. *R. ROBERT SCHELLENBERG1. & ...
Lymphocyte | Define Lymphocyte at Dictionary.com
Lymphocyte definition, a type of white blood cell having a large, spherical nucleus surrounded by a thin layer of nongranular ... lymphocyte. Historical Examples. of lymphocyte. *. When large forms of the lymphocyte are present, the distinction is often ... Origin of lymphocyte. First recorded in 1885-90; lympho- + -cyte. Related formslym·pho·cyt·ic [lim-fuh-sit-ik] /ˌlɪm fəˈsɪt ɪk/ ... a type of white blood cell formed in lymphoid tissueSee also B-lymphocyte, T-lymphocyte ...
CD4 Lymphocyte Monitoring | HealthCentral
Description Monitoring lymphocyte counts in a patient with HIV infection is one way to assess the degree of immunosuppression ... CD4 lymphocytes act as the on switch for part of the immune system, so as the number of CD4 cells drops, damage to the immune ... Monitoring lymphocyte counts in a patient with HIV infection is one way to assess the degree of immunosuppression and the risk ... HIV infects and kills certain white blood cells called CD4 lymphocytes, reducing their number. The number of CD4 cells usually ...
Lymphocytes From A Risk Group
Therefore, a large number of mutant T-lymphocytes with a person means that their owner has become a prey to irradiation or his/ ... Double increase of mutant lymphocyte frequency (more than 6.2 mutants per 10,000 cells) as compared to the background group was ... Increased frequency of mutations in patients lymphocytes is the evidence of risk. But, unfortunately, the lack of mutation ... Thus, European researchers have discovered that people with increased frequency of chromosomal abnormalities in lymphocytes of ...
T lymphocytes - definition of T lymphocytes by The Free Dictionary
T lymphocytes synonyms, T lymphocytes pronunciation, T lymphocytes translation, English dictionary definition of T lymphocytes ... n. Any of a class of lymphocytes, including the helper T cells and cytotoxic T lymphocytes, that form in bone marrow and mature ... Related to T lymphocytes: MHC, B lymphocytes, Cytotoxic T lymphocytes. T cell. n.. Any of a class of lymphocytes, including the ... T lymphocyte. lymph cell, lymphocyte - an agranulocytic leukocyte that normally makes up a quarter of the white blood cell ...
Review of Text-T Lymphocytes in the Liver
Bare lymphocyte syndrome type I: MedlinePlus Genetics
Bare lymphocyte syndrome type I (BLS I) is an inherited disorder of the immune system (primary immunodeficiency). ... Bare lymphocyte syndrome type I (BLS I) is an inherited disorder of the immune system (primary immunodeficiency). ... URL of this page: https://medlineplus.gov/genetics/condition/bare-lymphocyte-syndrome-type-i/ Bare lymphocyte syndrome type I. ... on cells, including infection-fighting white blood cells (lymphocytes), which is where the condition got its name. ...
Bare lymphocyte syndrome type II: MedlinePlus Genetics
Bare lymphocyte syndrome type II (BLS II) is an inherited disorder of the immune system categorized as a form of combined ... Genetic Testing Registry: Bare lymphocyte syndrome type 2, complementation group A *Genetic Testing Registry: Bare lymphocyte ... which leads to an absence of MHC class II proteins on the surface of certain lymphocytes. Lack of these proteins on lymphocytes ... Bare lymphocyte syndrome type II (BLS II) is an inherited disorder of the immune system categorized as a form of combined ...
NASA - ROle of Apoptosis in Lymphocyte Depression
ROle of Apoptosis in Lymphocyte Depression (ROALD) - 03.23.17. Overview , Description , Applications , Operations , Results , ... ROle of Apoptosis in Lymphocyte Depression (ROALD) will to determine the role of programmed cell death (apoptosis) in loss of T ... lymphocyte activity in microgravity.. * Various aspects of the apoptotic process will be assessed, using human T-lymphocytes, ... In this project, we plan to ascertain whether or not Space conditions might induce apoptosis in human lymphocytes through a 5- ...
Can Rheumatoid Arthritis Cause Low Lymphocytes?
Lymphocytes are white blood cells whose function is to fight off infection. There are three major categories of lymphocytes: B- ... Significance of Low Lymphocytes Acquired from RA or Its Treatment. Abnormally low lymphocytes, or lymphocytopenia, presents ... RA can cause low lymphocytes. Lymphocytes, sometimes referred to as "fighter cells," attack infection-causing microorganisms. ... Lymphocytes are a vital component of the bodys immune system. Medical intervention may be necessary if the number of ...
Autologous Lymphocytes - Medical Dictionary / Glossary | Medindia
Autologous Lymphocytes - A persons white blood cells. Lymphocytes have a number of roles in the immune system, including the ... Medical Word - Autologous Lymphocytes. Ans : A persons white blood cells. Lymphocytes have a number of roles in the immune ... Autologous Lymphocytes - Glossary. Written & Compiled by Medindia Content Team. Medically Reviewed by The Medindia Medical ...
Lymphocytes and Platelets | HowStuffWorks
Lymphocytes are complex cells that direct the bodys immune system. Learn about T lymphocyte types and functions, B lymphocytes ... Lymphocytes are complex cells that direct the bodys immune system. T lymphocytes start in the bone marrow from pluripotent ... T lymphocytes (T cells) are responsible for cell-mediated immunity. B lymphocytes are responsible for humoral immunity ( ... antibody production). Seventy-five percent of lymphocytes are T cells. Lymphocytes are different from the other WBCs because ...
AntigensProliferationTypes of lymphocytesSubsetsGranular lymphocytesLymphThymusMacrophagesTumor Infiltrating LymphocytesType of lymphocytePercentage of lymphocytesBone marrowCategories of lymphocytesLymphoidAntibodiesNodular lymphocyAbstractSmall lymphocytesNormal lymphocyteImmunologyInduce apoptosis in humanCellsResponsible for cell-mediatedReceptorBody'sHelperPeripheral blood lymphHumoral immunityScanning electron mRole of lymphocytesVitroStimulationSphingosine 1-phosphaDifferentiation and functionAntibodyPopulationsInfectionLymphocytosisAbsolute lymphocyteAbnormal lymphocyteAtypical lymphocytesCancerCountsTILsMurineMemory lymphocytesCytotoxic lymphocyteCellBacteriaVirusesBloodImmune responsesMainBloodstreamSearchProteinsViral
- Any of the lymphocytes that act to defend the body against disease by binding foreign antigens to receptors on the surface of their cells. (thefreedictionary.com)
- The most important effectors of the cell-mediated immune response, cytotoxic T lymphocytes , are the main immune cells that recognize foreign antigens on the surface of infected "self" cells. (thefreedictionary.com)
- Cantor H and Boyse EA (1975) Functional subclasses of T‐lymphocytes bearing different Ly antigens. (els.net)
- In their search for antigens, lymphocytes continuously shuttle among blood vessels, lymph vessels, and lymphatic tissues. (sciencemag.org)
- The role of lymphocytes involves recognizing harmful particles, or antigens, and carrying out processes to deal with them. (wisegeek.com)
- B lymphocytes become activated when they bind to their specific antigens. (wisegeek.com)
- The other type of cell produced by B lymphocytes, the memory cell, remembers antigens so that the body can respond more quickly if they attack again. (wisegeek.com)
- The data provide strong indications that one critical role of T-cell participation in humoral responses to antigens is to circumvent the development of a tolerogenic signal that, in the absence of such T-cell function, might otherwise ensue after binding of the antigenic determinants by specific precursor B lymphocytes. (rupress.org)
- A test for histocompatibility of HL-A antigens in which donor and recipient lymphocytes are mixed in culture. (dictionary.com)
- Sites on antigens that are recognized by T-lymphocytes . (citizendium.org)
- Special cytokines called interleukins that are produced by the T-4 lymphocytes mediate this proliferation. (encyclopedia.com)
- Lee SC, Sabath DE, Deutsch C, Prystowsky MB: Increased voltage-gated potassium conductance during interleukin 2-stimulated proliferation of a mouse helper T lymphocyte clone. (springer.com)
- Though there is no coherent explanation for these observations, and it is not known which biomolecules might act as gravity responders, recent evidence seems to suggest that inhibition of lymphocyte proliferation depends on alterations occurring within the first few hours of microgravity. (nasa.gov)
- Antigen receptor stimulation of mature alpha beta T lymphocytes can lead either to proliferation or death. (nih.gov)
- In 2012, National Jewish Health Advanced Diagnostic Laboratories introduced a nickel lymphocyte proliferation test (NiLPT) to detect nickel sensitization. (nationaljewish.org)
- Nickel lymphocyte proliferation testing is a clinically useful tool for evaluating patients undergoing initial joint replacement surgery or revision. (nationaljewish.org)
- Overall, the presence of B lymphocytes was associated with increased tissue proliferation, reduced cell death and a more supportive environment for wound healing. (eurekalert.org)
- So, I just followed but I can't get any lymphocytes proliferation after pulsed with BrdU. (protocol-online.org)
- This amino acid has been shown to play a role in lymphocyte proliferation, cytokine production by lymphocytes and macrophages and phagocytosis and superoxide production by macrophages and neutrophils. (scielo.br)
Types of lymphocytes5
- In this article, we look at different types of lymphocytes, what normal levels to have in the blood are, and what happens if levels get too low or too high. (medicalnewstoday.com)
- There are two main types of lymphocytes: T-cells and B-cells. (aaaai.org)
- The two main types of lymphocytes are T cells and B cells , which function in the adaptive immune system . (newworldencyclopedia.org)
- Different types of lymphocytes exist, known as T cells , B cells and natural killer cells, and their roles differ accordingly. (wisegeek.com)
- the common lymphoid progenitors (CLPs) can differentiate into all types of lymphocytes but lack the myeloid potential under physiological conditions, although some myeloid-related genes can be detected in CLPs depending on experimental conditions [ 1 , 2 ]. (intechopen.com)
- Prompted by advances in the understanding of defects in lymphocyte cytotoxicity, the discovery of adaptive natural killer (NK) cell subsets associated with certain viral infections, and compelling links between stress, adrenaline, and cytotoxic lymphocyte function, we reassessed the role of cytotoxic lymphocytes in ME/CFS. (frontiersin.org)
- Divergent hematological traits in swine may confer physiological differences, so we studied transcriptome profiles in spleens of Yorkshire pigs with different peripheral blood T lymphocyte subsets. (thefreedictionary.com)
- We will provide flow cytometry plots showing strategies to identify and characterize NK, T and B lymphocytes and their subsets in circulation. (intechopen.com)
- The NK cells are sometimes labeled "large granular lymphocytes," while the T cells and B cells are labeled as "small lymphocytes. (newworldencyclopedia.org)
- Large granular lymphocytes include natural killer cells (NK cells). (phys.org)
- Natural killer cells are large granular lymphocytes, with visible granules contained in their cytoplasm and a lobed nucleus. (wisegeek.com)
- Following maturation, the lymphocytes enter the circulation and peripheral lymphoid organs (e.g. the spleen and lymph nodes ) where they survey for invading pathogens and/or tumor cells. (wikipedia.org)
- A person with a high lymph count could be suffering from a condition called lymphocytosis, an elevated level of white blood cells called lymphocytes. (reference.com)
- Lymphocytes are found in the lymph nodes and spleen and circulate continuously in the blood and lymph. (dictionary.com)
- any of several closely related lymphocytes, developed in the thymus, that circulate in the blood and lymph and regulate the immune system's response to infected or malignant cells. (thefreedictionary.com)
- Lymphocytes continually pass back and forth between lymph tissue, lymph fluid and blood. (howstuffworks.com)
- Antibody blockade of IL-2 but not IL-4 reverses the marked reduction of lymph node V beta 8+ T cells caused in mice by the bacterial superantigen Staphylococcus aureus enterotoxin B. IL-2 may thus participate in a feedback regulatory mechanism by predisposing mature T lymphocytes to apoptosis. (nih.gov)
- Lymphocytes are produced inside bone marrow before traveling to areas of the lymphatic system such as the spleen, thymus and lymph nodes. (wisegeek.com)
- After they mature, the lymphocytes enter the circulation and lymphoid organs (e.g. the spleen and lymph nodes ) where they are able to sense invading pathogens and tumour cells. (wikipedia.org)
- Mesenteric lymph nodes show log-jamming of lymphocytes subjacent to sinus-lining endothelium. (ingentaconnect.com)
- The S1P receptor system may represent an early physiological link between the non-specific inflammatory response and the alteration of lymphocyte traffic through draining lymph nodes. (ingentaconnect.com)
- Surgery to remove the lymph nodes is the main treatment for stage I nodular lymphocyte-predominant childhood HL when doctors think the tumour can be completely removed, or resected. (cancer.ca)
- Mature lymphocytes recirculate via blood and lymph through lymphoid tissues in a relatively quiescent state until stimulated to proliferate during, for example, a bacterial or viral infection. (scielo.br)
- The WBC differential channel classifies Lymphocytes (LYMPH), Monocytes (MONO), Eosinophils (EO), and Granulocytes by cellular complexity and nucleic acid content. (crlcorp.com)
- B cells mature into B lymphocytes in the bursa equivalent, which in humans is the GALT , which is thought to be located in the Peyer's patches of the intestine,  while T cells migrate to and mature in a distinct organ, called the thymus . (wikipedia.org)
- Any of a class of lymphocytes, including the helper T cells and cytotoxic T lymphocytes, that form in bone marrow and mature in the thymus. (thefreedictionary.com)
- ROle of Apoptosis in Lymphocyte Depression (ROALD) will determine the role of programmed apoptosis (cell death) in loss of T-lymphocyte (white blood cells originating in the thymus) activity in microgravity. (nasa.gov)
- T lymphocytes start in the bone marrow from pluripotent hematopoietic stem cells, then travel to and mature in the thymus gland. (howstuffworks.com)
- CD8 T cells and B cells also differentiate into cytotoxic thymus‐derived lymphocytes and plasma cells, respectively, driven by specific activation in the context of CD4 T H cells (helper) and the cytokine microenvironment. (els.net)
- Together, the thymus and marrow produce approximately 109 mature lymphocytes each day, which are then released into the circulation. (amamanualofstyle.com)
- B cells mature into B lymphocytes in the bone marrow,  while T cells migrate to and mature in a distinct organ, called the thymus . (wikipedia.org)
- Lymphocyte differentiation in the rabbit thymus. (biomedsearch.com)
- IHC tested on neurological tissues highlighted a heterogeneous population of infiltrating T and B lymphocytes admixed macrophages. (nii.ac.jp)
- In the immune system such diverse cell types as T lymphocytes, B lymphocytes, macrophages, neutrophils, hybridomas, and natural killer cells express an assortment of channels (reviewed in 3). (springer.com)
- In this work, researchers have shown that in B lymphocytes HDAC7 is involved in the transcriptional repression of "inappropriate" gens characteristic of other cell types such as macrophages and T lymphocytes. (eurekalert.org)
- Eric Newsholme's laboratory was the first to show glutamine utilization by lymphocytes and macrophages. (scielo.br)
- Lymphocytes, macrophages and neutrophils play an important role in the immune and inflammatory response. (scielo.br)
- Indeed, it was not until the pioneering work of Eric Newsholme's laboratory in the 1980's that it was established that immune cells such as lymphocytes and macrophages could utilize glutamine at high rates in addition to glucose (3,4). (scielo.br)
- The metabolic fate of glutamine in lymphocytes, macrophages and neutrophils will be discussed in the present paper. (scielo.br)
Tumor Infiltrating Lymphocytes7
- Immunology of tumor infiltrating lymphocytes. (nih.gov)
- The tumor infiltrating lymphocytes (TIL) interact most closely with the tumor cells and are likely to more accurately reflect tumor host interactions. (nih.gov)
- Tumor Infiltrating Lymphocytes will be obtained from a small piece of tumour tissue removed from the invaded lymphnode after surgery. (clinicaltrials.gov)
- A preparation of cells, consisting of autologous tumor infiltrating lymphocytes, that are manipulated in vitro and, upon administration in vivo, re-infiltrate the tumor to initiate tumor cell lysis. (cancer.gov)
- Tumor-infiltrating lymphocytes are white blood cells that have left the bloodstream and migrated towards a tumor. (wikipedia.org)
- The 'Global Tumor Infiltrating Lymphocytes Immunotherapy Market Opportunity & Clinical Trials Insight 2025' report has been added to ResearchAndMarkets.com's offering. (yahoo.com)
- Now-a-days, the market of immunotherapy is more attributed towards developing Tumor-Infiltrating Lymphocytes for causing complete remission of the tumor cells from the cancer patients. (yahoo.com)
Type of lymphocyte3
- A third type of lymphocyte, known as a natural killer or NK cell, comes from the same place as B and T cells. (medicalnewstoday.com)
- A type of lymphocyte. (nih.gov)
- Some pathogens that influence the presence of this type of lymphocyte in the blood will also cause this altered cell to take on defining characteristics, such as changes to the shape of the nucleus and the quantity or color of the cytoplasm in the lymphocyte. (wisegeek.com)
Percentage of lymphocytes2
- B lymphocytes mature in the bone marrow. (howstuffworks.com)
- The journal 'Immunity' yesterday released a study showing strong evidence, and confirming a previous controversial study, that between initial and subsequent infection 80% of CD4 memory T-lymphocytes are located within bone marrow and not the circulatory system as has been widely believed by heath researchers. (chiro.org)
- Lymphoid hematopoiesis is not trivial, because although lymphocytes are found in the bloodstream and their precursor originates in the bone marrow, they mainly belong to the separate lymphatic system, which interacts with the blood circulation. (intechopen.com)
- Lymphocyte immune globulin is also used to treat aplastic anemia (a condition in which your bone marrow does not produce enough new blood cells). (rexhealth.com)
Categories of lymphocytes3
- There are two categories of lymphocytes known as B lymphocytes and T lymphocytes. (medicalnewstoday.com)
- There are three major categories of lymphocytes: B-cells, T-cells and NK-cells. (ehow.co.uk)
- The two main categories of lymphocytes are the B lymphocytes ( B cells ) and T lymphocytes ( T cell ), both of which are involved in the adaptive immune system (Alberts 1989). (newworldencyclopedia.org)
- All lymphocytes originate, during this process, from a common lymphoid progenitor before differentiating into their distinct lymphocyte types. (wikipedia.org)
- Homing of lymphocytes to lymphoid tissues is regulated by selective interactions between cell surface homing receptors and tissue vascular addressins at sites of lymphocyte recruitment from the blood. (nii.ac.jp)
- Lymphocytes belong to the lymphoid lineage and are considered as divergent from other blood cells lineages as those from the myeloid or erythroid lineage. (intechopen.com)
- Blood lymphocyte numbers, which are maintained by recirculation through secondary lymphoid organs, are essential for the efficient development of immune responses. (ingentaconnect.com)
- Effector lymphocytes function to eliminate the antigen, either by releasing antibodies (in the case of B cells), cytotoxic granules ( cytotoxic T cells ) or by signaling to other cells of the immune system ( helper T cells ). (wikipedia.org)
- Anti-lymphocyte globulin (ALG) is an infusion of animal- antibodies against human T cells which is used in the treatment of acute rejection in organ transplantation. (wikipedia.org)
- Lymphocytes have a number of roles in the immune system, including the production of antibodies and other substances that fight infection and disease. (medindia.net)
- Certain lymphocytes (called B cells) act by stimulating the production of antibodies. (thefreedictionary.com)
- Patients suffering from B-cell lymphomas can be treated with antibodies directed against the B-lymphocyte antigen CD20. (phys.org)
- One important role of lymphocytes is the production of antibodies, and plasma cells are responsible for this. (wisegeek.com)
- Stimulation of resting B lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces protein tyrosine phosphorylation, implicating one or more B-cell protein-tyrosine kinases (PTKs) in sIg signal transduction. (pnas.org)
- Researchers of the Cellular Differentiation Group at the Epigenetics and Cancer Biology Program at IDIBELL, led by researcher Maribel Parra, have identified a transcriptional repressor, the histone deacetylase HDAC7, involved in the generation and the identity of B lymphocytes, the cells responsible to create antibodies in our immune system. (eurekalert.org)
- In their report published in Wound Repair and Regeneration , the investigators from the MGH Vaccine and Immunotherapy Center (VIC) describe how application of mature B lymphocytes - the immune cells best known for producing antibodies - greatly accelerated the healing of acute and chronic wounds in both diabetic and nondiabetic mice. (eurekalert.org)
- Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is an uncommon subtype of Hodgkin lymphoma (HL). (uptodate.com)
- See 'Clinical manifestations, pathologic features, and diagnosis of nodular lymphocyte-predominant Hodgkin lymphoma' . (uptodate.com)
- The following are treatment options for childhood nodular lymphocyte-predominant Hodgkin lymphoma (HL). (cancer.ca)
- Also see Evans 2002 (3): "Our findings provide the first evidence that depression may alter the function of killer lymphocytes in HIV-infected women and suggest that depression may decrease natural killer cell activity and lead to an increase in activated CD8 T lymphocytes and viral load" (quote from the abstract). (thefreedictionary.com)
- Microscopically, in a Wright's stained peripheral blood smear , a normal lymphocyte has a large, dark-staining nucleus with little to no eosinophilic cytoplasm. (wikipedia.org)
- The normal lymphocyte range in adults is between 1,000 and 4,800 lymphocytes in 1 microliter (µL) of blood. (medicalnewstoday.com)
- The atypical lymphocyte has more cytoplasm and thus grows larger in size than a normal lymphocyte as a reaction to infection, hormone production, radiation or other factors that influence the immune system. (wisegeek.com)
- S. J. McLeod and M. R. Gold, "Activation and function of the Rap1 GTPase in B lymphocytes," International Reviews of Immunology , vol. 20, no. 6, pp. 763-789, 2001. (hindawi.com)
- Furthermore, the comprehension about lymphocytes and their contribution to the immune response will favor their application in developmental hematology and immunology. (intechopen.com)
Induce apoptosis in human2
- In this project, we plan to ascertain whether or not Space conditions might induce apoptosis in human lymphocytes through a 5-LOX-mediated pathway. (nasa.gov)
- To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. (mdpi.com)
- Increased expansions of adaptive NK cells or deviant cytotoxic lymphocyte adrenaline-mediated inhibition were not observed. (frontiersin.org)
- Lymphocytes include natural killer cells (which function in cell-mediated , cytotoxic innate immunity ), T cells (for cell-mediated , cytotoxic adaptive immunity ), and B cells (for humoral , antibody -driven adaptive immunity ). (wikipedia.org)
- A stained lymphocyte surrounded by red blood cells viewed using a light microscope . (wikipedia.org)
- The three major types of lymphocyte are T cells , B cells and natural killer (NK) cells. (wikipedia.org)
- A scanning electron microscope image of normal circulating human blood showing red blood cells , several types of white blood cells including lymphocytes, a monocyte , a neutrophil and many small disc-shaped platelets . (wikipedia.org)
- Lymphocytes are white blood cells that are also one of the body's main types of immune cells. (medicalnewstoday.com)
- The immune system is a complex network of cells known as immune cells that include lymphocytes. (medicalnewstoday.com)
- Lymphocytes are types of white blood cells that help the immune system defend against disease and infection. (aaaai.org)
- The T cells and the B cells together are called the lymphocytes. (encyclopedia.com)
- The large clone of identical lymphocytes then differentiates into different cells that can destroy the original antigen. (encyclopedia.com)
- The T-8 lymphocytes differentiate into cytotoxic T-lymphocytes (CTLs) that can destroy the body cells that have the original antigenic epitope on its surface, e.g., bacterial infected cells, viral infected cells, and tumor cells. (encyclopedia.com)
- Some of the T lymphocytes become memory cells. (encyclopedia.com)
- Lymphocytes are produced by the same stem cells that produce red blood cells. (reference.com)
- Lymphocytes normally represent 20 - 40% of circulating white blood cells. (reference.com)
- Locally resident intraepithelial lymphocytes (IELs) are primarily T cells with potent cytolytic and immunoregulatory capacities, which they use to sustain epithelial integrity. (nature.com)
- Yoshikai, Y. The interaction of intestinal epithelial cells and intraepithelial lymphocytes in host defense. (nature.com)
- Specific populations of T lymphocytes have emerged as important reparative cells with context-specific actions. (jci.org)
- for example, phagocytosis by rabbit polymorphonuclear leukocytes (PMNs) leads to a selective depletion of con A binding sites which is prevented by colchicine 1 , con A inhibition of lymphocyte immunoglobulin receptor capping is reversed by colchicine 2-4 and con A-induced aggregation of PMNs, fibroblasts and hepatoma cells is inhibited by colchicine 5-7 . (nature.com)
- HIV infects and kills certain white blood cells called CD4 lymphocytes, reducing their number. (healthcentral.com)
- CD4 lymphocytes act as the 'on switch' for part of the immune system , so as the number of CD4 cells drops, damage to the immune system may progress. (healthcentral.com)
- Double increase of mutant lymphocyte frequency (more than 6.2 mutants per 10,000 cells) as compared to the background group was discovered with 37 percent of cancer patients, and this result cannot be considered accidental. (innovations-report.com)
- Washington, January 4 ( ANI ): In a new study, researchers claim to have succeeded for the first time in creating cancer-specific, immune system cells called killer T lymphocytes , from induced pluripotent stem cells (iPS cells). (thefreedictionary.com)
- It contributes to the development of T lymphocytes , guard cells that participate in immune system reactions. (thefreedictionary.com)
- on cells, including infection-fighting white blood cells (lymphocytes), which is where the condition got its name. (medlineplus.gov)
- To help the body recognize and fight infections, MHC class II proteins on lymphocytes bind to fragments of proteins (peptides) from foreign invaders so that other specialized immune system cells can interact with them. (medlineplus.gov)
- When these immune system cells recognize the peptides as harmful, they trigger the lymphocytes to launch immune responses to get rid of the foreign invaders. (medlineplus.gov)
- It has been suggested that reduced growth response in lymphocytes during spaceflight might be linked to apoptosis, based on morphological anomalies and cDNA microarray analysis of space-flown human lymphoblastoid (Jurkat) cells. (nasa.gov)
- In this context, 5-Lipoxygenase (5-LOX) plays a central role in interleukin-2 expression and activation of human lymphocytes, and is involved in the initiation of programmed death (apoptosis) triggered by several stimuli in different human cells. (nasa.gov)
- Lymphocytes, sometimes referred to as "fighter cells," attack infection-causing microorganisms. (ehow.co.uk)
- Lymphocytes are white blood cells whose function is to fight off infection. (ehow.co.uk)
- Lymphocytes are complex cells that direct the body's immune system . (howstuffworks.com)
- T lymphocytes (T cells) are responsible for cell-mediated immunity. (howstuffworks.com)
- Seventy-five percent of lymphocytes are T cells. (howstuffworks.com)
- Both T lymphocytes (T cells) and B lymphocytes (B cells) can become memory cells. (nih.gov)
- Other lymphocyte-like cells are commonly known as natural killer cells, or NK cells, and are part of the innate immune system . (newworldencyclopedia.org)
- While offering protection against millions of possible invaders, including viruses , bacteria , are cancer cells, lymphocytes allow normal body cells and helpful, symbiotic bacteria to flourish at the same time. (newworldencyclopedia.org)
- In addition, lymphocyte-like cells called natural killer (NK) cells are involved in the immune system, albeit part of the innate immune system . (newworldencyclopedia.org)
- Lymphocytes are activated upon antigen (Ag) recognition by their clonotypic surface Ag receptors, TCR in the case of T cells and BCR in the case of B cells. (els.net)
- T lymphocyte cells form rosettes with sheep erythrocytes and, in the presence of transforming agents (mitogens), differentiate and divide. (fpnotebook.com)
- Phospholipid methylation in murine T lymphocytes but not B cells was stimulated by mitogenic lectins such as concanavalin A and phytohemagglutinin, and the methylation was then returned to the control level by the concomitant activation of phospholipase A2. (pnas.org)
- Deletion of GRK2 in lymphocytes compromises desensitization by high vascular S1P concentrations, thereby reducing responsiveness to the chemokine signal and trapping the cells in the vascular compartment. (sciencemag.org)
- The role of lymphocytes of the natural killer cell type is not as specific and they can recognize many different types of antigen , including infected cells and some tumor cells. (wisegeek.com)
- T lymphocytes can be subdivided into helper and killer T cells. (wisegeek.com)
- Helper T cells have what is probably the most important role of lymphocytes in the immune system. (wisegeek.com)
- Results were again significantly better than for l-PEI, although further research into the response of individual T cells to the transfection agent will be necessary, before either method can be used to routinely transfect primary T lymphocytes. (mdpi.com)
- A mathematical model for the interaction of between cancer cells and immune system, involving CML cancer cells, naive and effector T-lymphocytes. (ebi.ac.uk)
- The CD4 percentage is the percentage of CD4 cells in the total lymphocyte count . (thebody.com)
- Lymphocytes are the only cells in the body capable of specifically recognizing and distinguishing different antigenic determinants and are responsible for the two defining characteristics of the adaptive immune response, specificity and memory. (ozon.ru)
- Since lymphocytes play an integral role in the bodys immune response as well as have several important physiologic roles to play within the body, any aberration in these cells leads to widespread pathological conditions. (ozon.ru)
- Tristram G. Parslow(pp40-41) Lymphocytes are the cells that carry out antigen-specific immune responses. (amamanualofstyle.com)
- Some of the viruses that alter lymphocyte production in the body are the Epstein-Barr virus, cytomegalovirus, syphilis and hepatitis C . The Eppstein-Barr virus and cytomegalovirus atypical lymphocyte structures are often called Downey cells in honor of Hal Downey, who discovered them in 1923. (wisegeek.com)
- For example, infectious mononucleosis produces an atypical lymphocyte that has more cytoplasm than the usual white blood cells. (wisegeek.com)
- In addition to being larger than typical lymphocytes, these cells also exhibit the presence of nucleoli. (wisegeek.com)
- Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. (sciencemag.org)
- Our demonstration that B lymphocytes - immune system cells that are abundant in the blood - can accelerate wound healing in both healthy and diabetic skin potentially opens up an exciting path to a new treatment for chronic wounds, such as diabetic foot ulcers," says Ruxandra Sîrbulescu, PhD, of the MGH-VIC, lead and corresponding author of the report. (eurekalert.org)
- Look at percentage of cells within the lymphocyte gate that express the B-cell marker CD19, and compare to healthy controls and non-healthy controls. (clinicaltrials.gov)
- So you could try washing your stimulator cells before adding responders or you might have to add a peptide for lymphocyte recognition and response! (protocol-online.org)
- They are named "natural killer cells" because they do not require prior activation in order to kill cells which are missing MHC class I. The X lymphocyte is a reported cell type expressing both a B-cell receptor and T-cell receptor and is hypothesized to be implicated in type 1 diabetes. (wikipedia.org)
- T cells belong to a group of white blood cells known as lymphocytes, and play a central role in cell-mediated immunity. (medicalxpress.com)
- They can be distinguished from other lymphocyte types, such as B cells and natural killer cells by the presence of a special receptor on their cell surface called T cell receptors (TCR). (medicalxpress.com)
- The treatment regimen associated with the TIL therapy comprises of collection of lymphocytes that have the ability to engage themselves in the tumor cells destruction. (yahoo.com)
- Upon administration, BCMA-specific CAR-expressing T-lymphocytes specifically recognize and kill BCMA-expressing tumor cells. (cancer.gov)
Responsible for cell-mediated2
- According to Japanese and Russian researchers' data, the patients suffering from larynx cancer and some other malignant growths, even before treatment starts experience increase of quantity of lymphocytes with T-cell receptor (TCR) genes mutations. (innovations-report.com)
- Desensitization of a sphingosine 1-phosphate receptor enables lymphocytes to leave the vascular compartment. (sciencemag.org)
- Receptor-mediated formation, metabolism, and mode of action of these novel second messengers in T lymphocytes will be reviewed. (frontiersin.org)
- Recirculating populations of B and T lymphocytes are regulated by the sphingosine-1-phosphate (S1P) receptor-dependent control of lymphocyte egress. (ingentaconnect.com)
- Pharmacological subversion of the S1P receptor system, through systemic S1P agonist-induced inhibition of lymphocyte egress, suppresses antigenic responses to peripheral, but not to systemically, delivered antigen. (ingentaconnect.com)
- T lymphocytes that constitutively express the IL-2 receptor a-chain, CD25, and the transcription factor Foxp3, comprising approximately 10% of the [CD4.sup. (thefreelibrary.com)
- A preparation of autologous peripheral blood T-lymphocytes (PBTL) that have been genetically modified to express a chimeric antigen receptor (CAR) specific for the B cell maturation antigen (BCMA), with potential immunostimulating and antineoplastic activities. (cancer.gov)
- Lack of these proteins on lymphocytes impairs the body's immune response to bacteria, viruses, and fungi, leading to persistent infections in individuals with BLS II syndrome. (medlineplus.gov)
- Lymphocytes are a vital component of the body's immune system. (ehow.co.uk)
- Lymphocytes play an important and integral role in the body's defenses, and are part of a system of extraordinary complexity and coordination. (newworldencyclopedia.org)
- In addition to making changes in the structure of the body's lymphocytes, most viruses that can create an atypical lymphocyte are also transmitted through sex or the exchange of bodily fluids. (wisegeek.com)
- Lymphocyte immune globulin anti-thymocyte (also called equine anti-thymocyte immune globulin), is an immunosuppressant that lowers your body's immune system. (rexhealth.com)
- Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). (fpnotebook.com)
- T-helper cell type (Th)2 lymphocytes play an important role in the initiation, progression and persistence of allergic diseases, including asthma. (ersjournals.com)
- In contrast to strictly cytokine-producing helper ILCs, resident innate lymphocyte populations with cytolytic potential have been identified in multiple tissues in both mouse and human. (jimmunol.org)
Peripheral blood lymph1
- Frequently peripheral blood lymphocytes (PBL) do not reflect the tumor host relationship and cell mediated immunity in the PBL does not often correlate with prognosis. (nih.gov)
- B lymphocytes are responsible for humoral immunity (antibody production). (howstuffworks.com)
Scanning electron m1
- A scanning electron microscope (SEM) image of a single human lymphocyte. (wikipedia.org)
Role of lymphocytes3
- By comparison with controls, a significant slowing of the cell cycle of lymphocytes in vitro was observed in all FA patients, and possibly in FA heterozygotes, although to a lesser degree. (nih.gov)
- Lymphocyte stimulation leads to the activation of selected transcription factors depending on the type of signals. (els.net)
- Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases. (pnas.org)
- The presence of an atypical lymphocyte, otherwise referred to as a reactive lymphocyte or an atypical white blood cell, in the bloodstream is an indicator of antigen stimulation or immune system activation in the body. (wisegeek.com)
- Chemokines mediate entry of lymphocytes into lymphatic tissues, and sphingosine 1-phosphate (S1P) promotes localization of lymphocytes to the vasculature. (sciencemag.org)
Differentiation and function2
- In summary, employing sensitive assays and analyses for quantification of cytotoxic lymphocyte differentiation and function, cytotoxicity lymphocyte aberrances were not found among ME/CFS patients. (frontiersin.org)
- Lymphocyte differentiation and function is directed by specific transcription factors that determine the cell lineage. (els.net)
- Incited by the role of CD4+ T-lymphocytes in the production of immunoglobulin (Ig) E antibody by B-lymphocytes, subsequent studies in the late 1980s demonstrated that they are also involved in the pathophysiology of allergic asthma 2 , 3 . (ersjournals.com)
- This Review focuses on the major populations of T lymphocytes known to mediate tissue repair, their reparative mechanisms, and the diseases in which they have been implicated. (jci.org)
- Les thymocytes doubles n gatifs sont tout d'abord isol s, puis s par s en diverses sous populations (DN1, DN2, DN3 et DN4) suivant l'expression des marqueurs CD44 (une prot ine d'adh sion) et CD25 (cha ne α du r cepteur de l'IL-2). (unice.fr)
- Detection of gene expression specific for different kind of immune cell populations can then be used to determine the degree of lymphocyte infiltration as has been shown in breast cancer. (wikipedia.org)
- Lympho-depletion is thought to eliminate the negative effects of other lymphocytes that may compete for growth factors and decrease anti-tumor effects of the TILs, depleting regulatory or inhibitory lymphocyte populations. (wikipedia.org)
- A high lymphocyte count is referred to as lymphocytosis and is caused by a bacterial or viral infection, cancer, autoimmune disorders, tuberculosis, HIV/AI. (reference.com)
- Lymphocytes help fight off diseases, so it's normal to see a temporary increase after an infection. (reference.com)
- Monitoring lymphocyte counts in a patient with HIV infection is one way to assess the degree of immunosuppression and the risk of developing opportunistic infections. (healthcentral.com)
- At 3 days postinfection, neither DEP exposure nor Listeria infection resulted in significant changes in T lymphocytes when compared with the air-exposed, noninfected control (data not shown). (thefreedictionary.com)
- Lymphocytopenia, the medical term for a low number of lymphocytes circulating in the blood, places individuals at a high risk for infection. (ehow.co.uk)
- is normal for a chronic hiv infection to have atipical lymphocytes (betewn 1-2%) 2. (thebody.com)
- Memory lymphocytes can recognize an antigen introduced into the body during a prior infection or vaccination. (nih.gov)
- The shape, color and size of the lymphocyte can offer lab pathologists the opportunity to identify the source of the infection. (wisegeek.com)
- An increase in lymphocyte concentration is usually a sign of a viral infection (in some rare case, leukemias are found through an abnormally high lymphocyte count in an otherwise normal person). (wikipedia.org)
- HealthCentral explains that lymphocytosis, or the presence of a high number of lymphocytes, can be caused by many different disorders and diseases, including mononucleosis, toxoplasmosis, AIDS and tuberculosis. (reference.com)
- however, adults with more than 3,000 lymphocytes per milliliter of blood are generally considered to suffer from lymphocytosis. (reference.com)
- In some cases, children must have more than 9,000 lymphocytes per milliliter of blood to be considered to suffer from lymphocytosis. (reference.com)
- Although lymphocytes are always present in the bloodstream, interaction with immune system triggers is required to create an environment in which the abnormal lymphocyte is produced. (wisegeek.com)
- Yes atypical lymphocytes reflect the size and shape of lymphocytes as they can be seen under the microscope. (thebody.com)
- These viruses, which have the ability to remain dormant for many years, are often first identified by the presence of atypical lymphocytes in the blood. (wisegeek.com)
- I got to look at a few slides of atypical lymphocytes when I took Anatomy and Physiology. (wisegeek.com)
- She often has to look for atypical lymphocytes in her samples, among other atypical things! (wisegeek.com)
- As the article said, different atypical lymphocytes have different characteristics. (wisegeek.com)
- Thus, European researchers have discovered that people with increased frequency of chromosomal abnormalities in lymphocytes of blood more often fall ill with cancer. (innovations-report.com)
- These mutations are easy to discover via a special immunological test, which was used by the Obninsk physicians for search of mutant lymphocytes in blood of patients with thyroid gland cancer. (innovations-report.com)
- Unusually high or low lymphocyte counts can be a sign of disease. (medicalnewstoday.com)
- Children have much higher lymphocyte counts than adults, and the count tends to vary with age. (reference.com)
- CD4 counts and total lymphocyte count? (thebody.com)
- Higher white blood cell counts and the presence of atypical lymphocyte cell structures in the bloodstream are indicative of infections. (wisegeek.com)
- The process can also involve a preliminary chemotherapy regimen to deplete endogenous lymphocytes in order to provide the adoptively transferred TILs with enough access to surround the tumor sites. (wikipedia.org)
- Klein, J. R. Ontogeny of the Thy-1-, Lyt-2 + murine intestinal intraepithelial lymphocyte. (nature.com)
- DeCoursey TE, Chandy KG, Gupta S, Cahalan MD: Two types of potassium channels in murine T lymphocytes. (springer.com)
- DeCoursey TE, Chandy KG, Gupta S, Cahalan MD: Mitogen induction of ion channels in murine T lymphocytes. (springer.com)
- Lewis RS and Cahalan MD. Subset-specific expression of potassium channels in developing murine T lymphocytes. (springer.com)
- These results suggest that the mitogenesis of murine T lymphocytes is triggered by the activation of both phospholipid methyltransferase(s) and phospholipase A2. (pnas.org)
- Forty-eight patients from two independent cohorts fulfilling the Canada 2003 criteria for ME/CFS were evaluated with respect to cytotoxic lymphocyte phenotype and function. (frontiersin.org)
- Reproducible differences between patients and controls were not found in cytotoxic lymphocyte numbers, cytotoxic granule content, activation status, exocytotic capacity, target cell killing, or cytokine production. (frontiersin.org)
- A lymphocyte is one of the subtypes of a white blood cell in a vertebrate 's immune system . (wikipedia.org)
- This investigation looked at how spaceflight influences immune cell function, including the role of programmed cell death (apoptosis) in loss of T-lymphocytes, a type of white blood cell. (nasa.gov)
- Lymphocytes are one type of white blood cell. (thebody.com)
- ___ B cell → ___ B, importante en la producción de anticuerpos. (thefreedictionary.com)
- Lymphocyte activation triggers multiple signalling cascades that converge in the cell nucleus to cause significant changes in the pattern of gene expression that determine the phenotype of activated lymphocytes and, ultimately, the type of immune response. (els.net)
- A T lymphocyte is a type of white blood cell. (fpnotebook.com)
- Re: [Felvtalk] Immunoregolin or Lymphocyte T-cell immun. (mail-archive.com)
- The 2 main types are the T lymphocyte and the B lymphocyte, also called the T cell and the B cell. (amamanualofstyle.com)
- Crowding by the surrounding red blood cell is the reason why the lymphocytes produced by infectious mononucleosis often have a dented shape to their cytoplasm. (wisegeek.com)
- All lymphocytes come from a common basic lymphocyte cell before differentiating into their distinct lymphocyte types. (wikipedia.org)
- In normal situations, the coarse, dense nucleus of a lymphocyte is approximately the size of a red blood cell (about 7 μm in diameter). (wikipedia.org)
- Lymphocyte immune globulin is made of certain blood products from horses and may contain viruses and other infectious agents. (rexhealth.com)
- In children, the normal range is between 3,000 and 9,500 lymphocytes in 1 µL of blood. (medicalnewstoday.com)
- What is the cause of having a high lymphocyte count in the blood? (reference.com)
- In the F0 generation, we obtained 35 pups and 25 out of them were positive for Surveyor assay, and most of mutants displayed dramatic reductions of T and B lymphocytes in the peripheral blood. (nii.ac.jp)
- My daughter is about the same age and has chronic low lymphocytes results when her blood is drawn. (medhelp.org)
- Medical intervention may be necessary if the number of lymphocytes circulating in the blood becomes depressed. (ehow.co.uk)
- The effects of other virus or lymphocyte disorders can also often be estimated by counting the numbers of lymphocytes present in the blood . (wikipedia.org)
- While using lymphocyte immune globulin, you may need frequent blood tests. (rexhealth.com)
- T lymphocytes can also help control immune responses. (fpnotebook.com)
- these lymphocytes are the main agents of the acquired immune system. (wikipedia.org)
- Lymphocytopenia, also referred to as lymphopenia, occurs when your lymphocyte count in your bloodstream is lower than normal. (reference.com)
- These images are a random sampling from a Bing search on the term "T-Lymphocyte. (fpnotebook.com)
- Mutations in the CIITA , RFX5 , RFXANK , or RFXAP gene prevent transcription of MHC class II genes, which leads to an absence of MHC class II proteins on the surface of certain lymphocytes. (medlineplus.gov)
- The genetic modification of human T lymphocytes with established non-viral methods is inefficient. (mdpi.com)