Heterogeneous group of immunodeficiency syndromes characterized by hypogammaglobulinemia of most isotypes, variable B-cell defects, and the presence of recurrent bacterial infections.
An immunologic deficiency state characterized by an extremely low level of generally all classes of gamma-globulin in the blood.
A dysgammaglobulinemia characterized by a deficiency of IMMUNOGLOBULIN A.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A classification of B-lymphocytes based on structurally or functionally different populations of cells.
A dysgammaglobulinemia characterized by a deficiency of IMMUNOGLOBULIN G.
Syndromes in which there is a deficiency or defect in the mechanisms of immunity, either cellular or humoral.
Polyploidy with three sets of chromosomes. Triploidy in humans are 69XXX, 69XXY, and 69XYY. It is associated with HOLOPROSENCEPHALY; ABNORMALITIES, MULTIPLE; PARTIAL HYDATIDIFORM MOLE; and MISCARRAGES.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
An island in the Gulf of St. Lawrence constituting a province of Canada in the eastern part of the country. It is very irregular in shape with many deep inlets. Its capital is Charlottetown. Discovered by the French in 1534 and originally named Ile Saint-Jean, it was renamed in 1799 in honor of Prince Edward, fourth son of George III and future father of Queen Victoria. (From Webster's New Geographical Dictionary, 1988, p981 & Room, Brewer's Dictionary of Names, 1992, p433)
An order of the class Insecta. Wings, when present, number two and distinguish Diptera from other so-called flies, while the halteres, or reduced hindwings, separate Diptera from other insects with one pair of wings. The order includes the families Calliphoridae, Oestridae, Phoridae, SARCOPHAGIDAE, Scatophagidae, Sciaridae, SIMULIIDAE, Tabanidae, Therevidae, Trypetidae, CERATOPOGONIDAE; CHIRONOMIDAE; CULICIDAE; DROSOPHILIDAE; GLOSSINIDAE; MUSCIDAE; TEPHRITIDAE; and PSYCHODIDAE. The larval form of Diptera species are called maggots (see LARVA).
A component of the PUBLIC HEALTH SERVICE that provides leadership related to the delivery of health services and the requirements for and distribution of health resources, including manpower training.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Central nervous system vasculitis that is associated with SYSTEMIC LUPUS ERYTHEMATOSUS. Clinical manifestations may include DEMENTIA; SEIZURES; CRANIAL NERVE DISEASES; HEMIPARESIS; BLINDNESS; DYSPHASIA; and other neurological disorders.
Glomerulonephritis associated with autoimmune disease SYSTEMIC LUPUS ERYTHEMATOSUS. Lupus nephritis is histologically classified into 6 classes: class I - normal glomeruli, class II - pure mesangial alterations, class III - focal segmental glomerulonephritis, class IV - diffuse glomerulonephritis, class V - diffuse membranous glomerulonephritis, and class VI - advanced sclerosing glomerulonephritis (The World Health Organization classification 1982).
A classification of lymphocytes based on structurally or functionally different populations of cells.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
A PREDNISOLONE derivative with similar anti-inflammatory action.
A synthetic anti-inflammatory glucocorticoid derived from CORTISONE. It is biologically inert and converted to PREDNISOLONE in the liver.
A condition characterized by the presence of abnormal quantities of CRYOGLOBULINS in the blood. Upon cold exposure, these abnormal proteins precipitate into the microvasculature leading to restricted blood flow in the exposed areas.
Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS.
The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.
ERYTHROCYTE size and HEMOGLOBIN content or concentration, usually derived from ERYTHROCYTE COUNT; BLOOD hemoglobin concentration; and HEMATOCRIT. The indices include the mean corpuscular volume (MCV), the mean corpuscular hemoglobin (MCH), and the mean corpuscular hemoglobin concentration (MCHC).
A plant genus of the family POACEAE. Folin is the water-soluble extract from Sasa albomarginata. Sasa kurinensis is an ingredient of Sho-ju-sen, a Japanese herbal medicine.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.

Crystal structure of an MHC class I presented glycopeptide that generates carbohydrate-specific CTL. (1/8823)

T cell receptor (TCR) recognition of nonpeptidic and modified peptide antigens has been recently uncovered but is still poorly understood. Immunization with an H-2Kb-restricted glycopeptide RGY8-6H-Gal2 generates a population of cytotoxic T cells that express both alpha/beta TCR, specific for glycopeptide, and gamma/delta TCR, specific for the disaccharide, even on glycolipids. The crystal structure of Kb/RGY8-6H-Gal2 now demonstrates that the peptide and H-2Kb structures are unaffected by the peptide glycosylation, but the central region of the putative TCR binding site is dominated by the extensive exposure of the tethered carbohydrate. These features of the Kb/RGY8-6H-Gal2 structure are consistent with the individual ligand binding preferences identified for the alpha/beta and gamma/delta TCRs and thus explain the generation of a carbohydrate-specific T cell response.  (+info)

T cell subsets in experimental lupus nephritis: modulation by bacterial superantigen. (2/8823)

Chronic graft-vs-host disease (GvH), induced by injection of DBA/2 lymphocytes into (C57BL/6 x DBA/2)F1 hybrids, is a murine model for lupus nephritis, associated with a Th2-dependent polyclonal B cell activation. The development of glomerulosclerosis in this model is preceded by a glomerular influx of LFA-1+ T cells. We investigated whether exposure to bacterial superantigen would modulate the course of this autoimmune syndrome. Injection of the bacterial superantigen staphylococcal enterotoxin B (SEB) in mice has been shown to induce the activation of TcRVbeta8+ T cells. Within 2 weeks after GvH induction, mice were injected twice with 20 microg of SEB and the following parameters were examined: cytokine and Ig profile, proteinuria and renal pathology. The second SEB injection induced in GvH mice an increased release of both interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) as compared with control F1 mice. No differences were observed in IL-2 production. SEB-treated GvH mice demonstrated a delayed onset of proteinuria. Histological analysis of the kidney showed that SEB-challenged GvH mice displayed significantly more interstitial inflammation and mesangial proliferation together with more IgG2a deposits in glomeruli than non-injected GvH mice. From these results, we conclude that GvH mice are more responsive to SEB in terms of cytokine production and that bacterial infection can modulate the course of this renal disease from a membranous to a more proliferative type of nephropathy.  (+info)

Melanoma cells present a MAGE-3 epitope to CD4(+) cytotoxic T cells in association with histocompatibility leukocyte antigen DR11. (3/8823)

In this study we used TEPITOPE, a new epitope prediction software, to identify sequence segments on the MAGE-3 protein with promiscuous binding to histocompatibility leukocyte antigen (HLA)-DR molecules. Synthetic peptides corresponding to the identified sequences were synthesized and used to propagate CD4(+) T cells from the blood of a healthy donor. CD4(+) T cells strongly recognized MAGE-3281-295 and, to a lesser extent, MAGE-3141-155 and MAGE-3146-160. Moreover, CD4(+) T cells proliferated in the presence of recombinant MAGE-3 after processing and presentation by autologous antigen presenting cells, demonstrating that the MAGE-3 epitopes recognized are naturally processed. CD4(+) T cells, mostly of the T helper 1 type, showed specific lytic activity against HLA-DR11/MAGE-3-positive melanoma cells. Cold target inhibition experiments demonstrated indeed that the CD4(+) T cells recognized MAGE-3281-295 in association with HLA-DR11 on melanoma cells. This is the first evidence that a tumor-specific shared antigen forms CD4(+) T cell epitopes. Furthermore, we validated the use of algorithms for the prediction of promiscuous CD4(+) T cell epitopes, thus opening the possibility of wide application to other tumor-associated antigens. These results have direct implications for cancer immunotherapy in the design of peptide-based vaccines with tumor-specific CD4(+) T cell epitopes.  (+info)

Peripheral autoantigen induces regulatory T cells that prevent autoimmunity. (4/8823)

Previous studies have shown that autoimmune thyroiditis can be induced in normal laboratory rats after thymectomy and split dose gamma-irradiation. Development of disease can be prevented by reconstitution of PVG rats shortly after their final irradiation with either peripheral CD4(+)CD45RC- T cells or CD4(+)CD8(-) thymocytes from syngeneic donors. Although the activity of both populations is known to depend on the activities of endogenously produced interleukin 4 and transforming growth factor beta, implying a common mechanism, the issue of antigen specificity of the cells involved has not yet been addressed. In this study, we show that the regulatory T cells that prevent autoimmune thyroiditis are generated in vivo only when the relevant autoantigen is also present. Peripheral CD4(+) T cells, from rats whose thyroids were ablated in utero by treatment with 131I, were unable to prevent disease development upon adoptive transfer into thymectomized and irradiated recipients. This regulatory deficit is specific for thyroid autoimmunity, since CD4(+) T cells from 131I-treated PVG.RT1(u) rats were as effective as those from normal donors at preventing diabetes in thymectomized and irradiated PVG.RT1(u) rats. Significantly, in contrast to the peripheral CD4(+) T cells, CD4(+)CD8(-) thymocytes from 131I-treated PVG donors were still able to prevent thyroiditis upon adoptive transfer. Taken together, these data indicate that it is the peripheral autoantigen itself that stimulates the generation of the appropriate regulatory cells from thymic emigrant precursors.  (+info)

Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine. (5/8823)

Helper T cells are classified into Th1 and Th2 subsets based on their profiles of cytokine production. Th1 cells are involved in cell-mediated immunity, whereas Th2 cells induce humoral responses. Selective recruitment of these two subsets depends on specific adhesion molecules and specific chemoattractants. Here, we demonstrate that the T cell-directed CC chemokine thymus and activation-regulated chemokine (TARC) was abundantly produced by monocytes treated with granulocyte macrophage colony stimulating factor (GM-CSF) or IL-3, especially in the presence of IL-4 and by dendritic cells derived from monocytes cultured with GM-CSF + IL-4. The receptor for TARC and another macrophage/dendritic cell-derived CC chemokine macrophage-derived chemokine (MDC) is CCR4, a G protein-coupled receptor. CCR4 was found to be expressed on approximately 20% of adult peripheral blood effector/memory CD4+ T cells. T cells attracted by TARC and MDC generated cell lines predominantly producing Th2-type cytokines, IL-4 and IL-5. Fractionated CCR4+ cells but not CCR4- cells also selectively gave rise to Th2-type cell lines. When naive CD4+ T cells from adult peripheral blood were polarized in vitro, Th2-type cells selectively expressed CCR4 and vigorously migrated toward TARC and MDC. Taken together, CCR4 is selectively expressed on Th2-type T cells and antigen-presenting cells may recruit Th2 cells expressing CCR4 by producing TARC and MDC in Th2-dominant conditions.  (+info)

Development and function of autospecific dual TCR+ T lymphocytes. (6/8823)

Recent studies have challenged the long held concept that each T lymphocyte expresses on its surface only a single, unique alphabetaTCR. Dual TCR+ T cells have been recognized, however, their origin and potential to escape screening for self-reactivity remain obscure. We now report the thymic generation of dual alphabetaTCR+ T cells in the H-2Db/H-Y-specific TCR transgenic (Tg) mouse. Dual TCR+ thymocytes were positively selected less efficiently than single TCR+ thymocytes, although a subset attained maturity. Importantly, when TCR Tg mice were bred onto a negatively selecting background, auto-specific cells survived central deletion and matured as CD4+ dual TCR+ cells. These cells were autoreactive when CD8 expression was restored. The existence of autospecific, dual TCR+ T cells may have implications for the maintenance of self tolerance.  (+info)

Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. (7/8823)

Signaling through A2A adenosine receptors (A2AR) regulates T lymphocyte expansion and modulates T cell receptor (TCR)-mediated effector functions in vitro. To understand the role of A2ARs in the regulation of immune response, we investigated the expression levels of this receptor in different functional lymphocyte subsets. Monoclonal anti-A2AR antibody was used to develop a flow cytometric assay to quantify the expression A2ARs on lymphocytes. We report that detectable levels of expression of A2ARs are much higher among T cells than B cells. More CD4(+) than CD8(+) T cells express A2ARs, but activation of T cells increases A2AR expression, predominantly in CD8(+) T cells. No significant differences were found in the proportion of A2AR+ cells between CD8(low) and CD8(high) T cells or between TCR/CD3(low) and TCR/CD3(high) T cells. Studies of T helper cell subsets (TH1 and TH2) reveal that lymphokine-producing cells are much more likely to express A2ARs than are cells that do not produce lymphokines. These results suggest that A2ARs are variably expressed on T cell subsets and may regulate cytokine production in activated T lymphocytes.  (+info)

IgA production in MHC class II-deficient mice is primarily a function of B-1a cells. (8/8823)

Mice deficient in MHC class II expression (C2d mice) do not make antibody to protein antigens administered systemically, but their ability to produce IgA antibody to antigen administered at mucosal sites has not been described. We investigated IgA production by C2d mice and their IgA antibody response to antigen given orally. Young C2d mice had normal amounts of serum IgA, intestinal-secreted IgA and normal numbers of intestinal IgA plasma cells, compared to control C57BL/6 mice. IgA production by C2d mice increased with age. Following oral immunization with cholera toxin, C57BL/6 mice responded with IgA and IgG antibody, and had increased numbers of IgA plasma cells, but C2d mice gave no response. The Peyer's patch and mesenteric lymph node tissues of C2d mice contained very few CD4-expressing T cells. Thus, C2d mice have no typical mucosal CD4 Th cells and cannot respond to a strong oral immunogen, yet they still produced and secreted IgA. We hypothesized that B-1 lymphocytes could provide a source of IgA independent of antigen-specific T cell help. Young C2d mice had normal numbers of peritoneal B-1a cells and their frequency increased with age. To test the role of these B-1a cells, we bred C2d mice to obtain mice that had no MHC class II expression and expressed the Xid gene that confers deficiency in B-1a cells. These double-deficient mice had 10-fold less serum and secreted IgA than all other F2 littermates. We conclude that B-1a cells are essential for the majority of IgA production in C2d mice. Thus, the C2d mouse may provide a useful tool for analysis of the role of intestinal IgA provided by B-1a cells.  (+info)

Zhang Y, Feng ZP, Naselli G, Bell F, Wettenhall J, Auyeung P, Ellis JA, Ponsonby AL, Speed TP, Chong MM, Harrison LC. MicroRNAs in CD4(+) T cell subsets are markers of disease risk and T cell dysfunction in individuals at risk for type 1 diabetes. Journal of autoimmunity 68 : 52 - 61(2016) PubMed (Grant IDs: 637338, 1004541, 1037321, 1026349 ...
A broad T cell receptor (TCR-) repertoire is required for an effective immune response. TCR-repertoire diversity declines with age. End-stage renal disease (ESRD) patients have a prematurely aged T cell system which is associated with defective T cell- mediated immunity. Recently, we showed that ESRD may significantly skew the TCR Vb-repertoire. Here we assessed the impact of ESRD on the TCR Vb-repertoire within different T cell subsets using a multi-parameter flow-cytometry-based assay, controlling for effects of ageing and CMV latency. Percentages of 24 different TCR Vb-families were tested in circulating naïve and memory T cell subsets of 10 ESRD patients and 10 age- and CMV-serostatus-matched healthy individuals (HI). The Gini-index, a parameter used in economics to describe the distribution of income, was calculated to determine the extent of skewing at the subset level taking into account frequencies of all 24 TCR Vb-families. In addition, using HI as reference population, the differential impact
Richard Jefferys, TAG. Recent research involving SIV-infected macaques has suggested that the early loss of a particular type of memory CD4 T cell (known as a central memory T cell or Tcm) may be a key predictor of the subsequent pace of disease progression. Tcm are a long-lived subset of memory T cells that can proliferate robustly in response to antigen. Tcm proliferation generates a fleet of T cells belonging to a shorter-lived subset called effector memory (Tem) cells. Tem are generally viewed as first-responders that can rapidly execute anti-pathogen functions, while Tcm provide a stem-cell like renewal source for new Tem if their numbers need to be bolstered. Studies in HIV-infected people have consistently shown a loss of Tcm and increase in Tem (which equates to a decrease in long-lived resting T cells and an increase in short-lived activated T cells), but whether changes in the numbers of different T cell subsets during early infection can predict disease progression has not been ...
Virgin and memory T cells reciprocally express high levels of the RA or the RO isoforms of CD45, respectively. In an examination of T cell expression of these two CD45 isoforms during human development, the RO+RA- memory T cells were infrequent in the newborn blood and spleen, but comprised approximately half of the T cells in adult tissues. These anticipated findings probably reflect the immunologic naivete of the newborn. Surprisingly, however, RO+RA- T cells were relatively abundant in fetal spleen and in cord blood samples from premature births, comprising approximately 25% and 10% of the T cells in these tissues, respectively. This early peripheral wave of RO+RA- T cells was composed of polyclonal T cells in both the CD4 and CD8 subpopulations. The fetal RO+ cells of CD4+ phenotype frequently expressed the CD25-alpha chain subunits that characterize high affinity IL-2 receptors, and were able to proliferate in response to exogenous IL-2. In further contrast with their RO+ memory T cell ...
A set of non-volatile storage elements is divided into subsets for erasing in order to avoid over-erasing faster erasing storage elements. The entire set of elements is erased until a first subset of the set of elements is verified as erased. The first subset can include the faster erasing cells. Verifying the first subset includes excluding a second subset from verification. After the first subset is verified as erased, they are inhibited from erasing while the second subset is further erased. The set of elements is verified as erased when the second subset is verified as erased. Verifying that the set of elements is erased can include excluding the first subset from verification or verifying both the first and second subsets together. Different step sizes are used, depending on which subset is being erased and verified in order to more efficiently and accurately erase the set of elements.
TY - JOUR. T1 - Identification of functional subsets by flow cytometry. T2 - Intracellular detection of cytokine expression. AU - Maino, Vernon C.. AU - Picker, Louis J.. N1 - Copyright: Copyright 2007 Elsevier B.V., All rights reserved.. PY - 1998/10/15. Y1 - 1998/10/15. N2 - Methods for analysis of T cell function have traditionally relied upon measurements of proliferation or cytokine expression in bulk cultures of PBMC in long term incubations with polyclonal mitogens or putative antigen. These techniques suffer from the drawback that they do not enable analysis of single cell responses in the context of unselected cellular backgrounds. In addition these methods are not sensitive enough to rapidly assess rare event responses characteristic of cognate memory T cell responses. This review discusses recently developed flow cytometric methods designed to rapidly assess leukocyte subset cytokine responses to polyclonal activators and specific antigen in PBMC and whole blood samples. These ...
The C-type lectin CD161 is expressed by a large proportion of human T lymphocytes of all lineages, including a population known as mucosal-associated invariant T (MAIT) cells. To understand whether different T cell subsets expressing CD161 have similar properties, we examined these populations in parallel using mass cytometry and mRNA microarray approaches. The analysis identified a conserved CD161++/MAIT cell transcriptional signature enriched in CD161+CD8+ T cells, which can be extended to CD161+ CD4+ and CD161+TCR gamma delta+ T cells. Furthermore, this led to the identification of a shared innate-like, TCR-independent response to interleukin (IL)-12 plus IL-18 by different CD161-expressing T cell populations. This response was independent of regulation by CD161, which acted as a costimulatory molecule in the context of T cell receptor stimulation. Expression of CD161 hence identifies a transcriptional and functional phenotype, shared across human T lymphocytes and independent of both T cell ...
Our analysis of DNTC suggests that they represent a novel suppressor cell population whose function is to negatively regulate immune responses. As DNTC acquire regulatory properties only after exposure to self Ags in vivo, it suggests that a self Ag-driven peripheral expansion plays a pivotal role in the acquisition of immunoregulatory function. Rather than stimulating rapid growth, effector cell formation, and acute activation markers, exposure to cognate Ag initiates DNTC to undergo slow expansion and memory conversion. For instance, DNTC having undergone self Ag-driven peripheral expansion possess markers of T cell memory, a lowered threshold of activation, an ability to rapidly express IFN-γ after TCR stimulation, and ex vivo cytolytic activity. However, DNTC from Ag+ mice differ from conventional T cells or those from Ag− mice in that they respond poorly when challenged with cognate Ag in vitro and fail to synthesize IL-2. As they possess potent cytolytic activity and express cognate Ag, ...
CD3gamma and CD3delta are two highly related components of the T cell receptor (TCR)-CD3 complex which is essential for the assembly and signal transduction of the T cell receptor on mature T cells. In gene knockout mice deficient in either CD3delta or CD3gamma, early thymic development mediated by pre-TCR was either undisturbed or severely blocked, respectively, and small numbers of TCR-alphabeta+ T cells were detected in the periphery of both mice. gammadelta T cell development was either normal in CD3delta-/- mice or partially blocked in CD3gamma-/- mice. To examine the collective role of CD3gamma and CD3delta in the assembly and function of pre-TCR and in the development of gammadelta T cells, we generated a mouse strain with a disruption in both CD3gamma and CD3delta genes (CD3gammadelta-/-). In contrast to mice deficient in either CD3gamma or CD3delta chains, early thymic development mediated by pre-TCR is completely blocked, and TCR-alphabeta+ or TCR-gammadelta+ T cells were absent in the
It has been unquestionably useful in advancing our understanding of immune cell function to recognize that B cells, T cells, and different T cell subsets (CD4+, CD8+, α/β, γ/δ, and natural killer T cells) constitute distinct lineages that do not interconvert. Although new subsets of TH cells continue to be elevated to the status of lineages, there are really no accepted criteria for what qualifies a cell for this august designation. The selective production of cytokines is the sine qua non for a subset, but this definition is tautologic and consequently leads to considerable confusion. TH1 cells make IFN-γ, but are all IFN-γ-producing CD4+ T cells TH1 cells? IL-22-producing cells make IFN-γ, but are they then TH1s or TH22s? Can a cell be a TH1/TH22, or should this be a new lineage? In the simplest view, TH cell lineages express lineage-defining transcription factors, but, as discussed, we know that transcription factor expression is dynamic; a particular subset can express more than ...
CD4+ T cells are critical for host defense but are also major drivers of immune-mediated disease. These T cells specialize to become distinct subsets and produce restricted patterns of cytokines, which are tailored to combat various microbial pathogens. Although classically viewed as distinct lineages, recent work calls into question whether helper CD4+ T cell subsets are more appropriately viewed as terminally differentiated cells or works in progress. Herein, we review recent advances that pertain to this topic and the mechanisms that contribute to helper CD4+ T cell commitment and plasticity. The therapeutic implications of these new findings are also considered.. ...
Immune cells act as TSA screeners in the intestine-tolerating commensal microbes and food antigens but remaining vigilant against attack. However, it has remained unclear how T cell precursors differentiate into the different cell types required to perform this balancing act. Now, Bilate et al. report that the T cell receptor (TCR) itself does not limit T cell fate to a single identity. The authors found that cells expressing a single TCR derived from a peripheral regulatory T cell (Treg) developed into either Tregs or CD4+CD8αα+ intraepithelial lymphocytes (CD4IELs) in the gut. This differentiation depended on cues from both the microbiota and the surrounding environment. ...
Phenotypic and functional properties of gammadelta T cells, which play an important role in mucocutaneous immunity, were examined to elucidate whether immunological abnormality in Behcets disease may be related to a specific T cell population. We found that CD45RA+ Vgamma9+ Vdelta2+ gammadelta T ce …
Citation: N/A Interpretive Summary: Technical Abstract: In order to identify cytokines with immunoregulatory functions in chickens, CD4+ hybridoma designated as P34 which spontaneously secretes various cytokines was developed. Rabbit antibody was developed against a P34 culture supernatant fraction with T-cell promoting activity and used to screen the P34 cDNA library. A cDNA encoding a 12kDa protein was identified and cloned into pBluescript vector. The amino acid sequence deduced from the cloned cDNA indicated a 143-amino acid precursor peptide. This 12kDa protein was expressed in many tissues including spleen, intestine and muscle using a reverse transcription-polymerase chain reaction (RT- PCR). Activation of spleen cells with concanavalin A enhanced the expression of this protein in a time dependent manner in Northern blot. Stable transfection of this gene in CHO and SF9 cells produced a biologically active protein which supported the growth of gamma delta T lymphocytes. In summary, a ...
Ana A. Weil, Mohammad Arifuzzaman, Taufiqur R. Bhuiyan, Regina C. LaRocque, Aaron M. Harris, Emily A. Kendall, Azim Hossain, Abdullah A. Tarique, Alaullah Sheikh, Fahima Chowdhury, Ashraful I. Khan, Farhan Murshed, Kenneth C. Parker, Kalyan K. Banerjee, Edward T. Ryan, Jason B. Harris, Firdausi Qadri, Stephen B. Calderwood ...
Unconventional T cells Introduction T lymphocytes are characterized by the expression of the CD3 molecule and the associated T cell receptor for antigen
T cells are central to the cell-mediated immune response. There are many different types of T cells, all derived from same lymphoid stem cell. T cell function, lineage and the T cell receptor are discussed, along with markers and antibodies used to define them.
Description of disease T cell, peripheral. Treatment T cell, peripheral. Symptoms and causes T cell, peripheral Prophylaxis T cell, peripheral
These cells inhibit the activation phase of the immune response by suppressing the response of B cells (antibody producing cells) or of other T cells (killer T cells, helper T cells) to an antigen resulting in tolerance for the antigen by the host ...
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The immune system cells known as T cells play a central role in the bodys ability to fight infections and cancer. For decades, however, details of the molecular signaling process that leads to T cell activation have remained a mystery.
Miltenyi Biotec offers synergistic and fully translational solutions for every step of the Research CAR T Cell Workflow. - Ísland
Looking for delta T lymphocyte? Find out information about delta T lymphocyte. a deposit of clay, silt, and sand formed at the mouth of a river where the stream loses velocity and drops part of its sediment load. No delta is formed if... Explanation of delta T lymphocyte
Gamma delta T cells (γδ T cells) are T cells that have a distinctive T-cell receptor (TCR) on their surface. Most T cells are αβ (alpha beta) T cells with TCR composed of two glycoprotein chains called α (alpha) and β (beta) TCR chains. In contrast, gamma delta (γδ) T cells have a TCR that is made up of one γ (gamma) chain and one δ (delta) chain. This group of T cells is usually much less common than αβ T cells, but are at their highest abundance in the gut mucosa, within a population of lymphocytes known as intraepithelial lymphocytes (IELs). The antigenic molecules that activate gamma delta T cells are still largely unknown. However, γδ T cells are peculiar in that they do not seem to require antigen processing and major-histocompatibility-complex (MHC) presentation of peptide epitopes, although some recognize MHC class Ib molecules. Furthermore, γδ T cells are believed to have a prominent role in recognition of lipid antigens. They are of an invariant nature and may be ...
CD4-CD8- (double negative [DN]) alpha/beta T cells are a largely uncharacterized subpopulation of unknown function. To investigate whether these cells are selected to recognize particular antigens or antigen-presenting molecules, DN alpha/beta T cells were purified from the peripheral blood of five normal donors and their T cell receptor (TCR) alpha and beta chains were examined. Random cloning of TCR alpha chains by single-sided polymerase chain reaction (PCR) amplification identified an invariant rearrangement between V alpha 24 and J alpha Q, with no N region diversity, which was expressed preferentially by DN alpha/beta T cells from all donors. Random cloning also identified a precise V alpha 7.2-J alpha (IGRJa14) rearrangement, with two variable amino acids encoded in the V-J junction, which was enriched in the DN alpha/beta T cell preparations from some, but not all, donors. Analysis of TCR beta chains by quantitative PCR amplification demonstrated that the expression of four V beta gene ...
TY - JOUR. T1 - Altered homeostasis and development of regulatory T cell subsets represent an IL-2R-dependent risk for diabetes in NOD mice. AU - Dwyer, Connor J.. AU - Bayer, Allison L. AU - Fotino, Carmen. AU - Yu, Liping. AU - Cabello-Kindelan, Cecilia. AU - Ward, Natasha C.. AU - Toomer, Kevin H.. AU - Chen, Zhibin. AU - Malek, Thomas. PY - 2017/12/19. Y1 - 2017/12/19. N2 - The cytokine interleukin-2 (IL-2) is critical for the functions of regulatory T cells (Tregs). The contribution of polymorphisms in the gene encoding the IL-2 receptor subunit (IL2RA), which are associated with type 1 diabetes, is difficult to determine because autoimmunity depends on variations in multiple genes, where the contribution of any one gene product is small. We investigated the mechanisms whereby a modest reduction in IL-2R signaling selectively in T lymphocytes influenced the development of diabetes in the NOD mouse model. The sensitivity of IL-2R signaling was reduced by about two- to threefold in Tregs from ...
CD4-CD8- (double negative [DN]) alpha/beta T cells are a largely uncharacterized subpopulation of unknown function. To investigate whether these cells are selected to recognize particular antigens or antigen-presenting molecules, DN alpha/beta T cells were purified from the peripheral blood of five normal donors and their T cell receptor (TCR) alpha and beta chains were examined. Random cloning of TCR alpha chains by single-sided polymerase chain reaction (PCR) amplification identified an invariant rearrangement between V alpha 24 and J alpha Q, with no N region diversity, which was expressed preferentially by DN alpha/beta T cells from all donors. Random cloning also identified a precise V alpha 7.2-J alpha (IGRJa14) rearrangement, with two variable amino acids encoded in the V-J junction, which was enriched in the DN alpha/beta T cell preparations from some, but not all, donors. Analysis of TCR beta chains by quantitative PCR amplification demonstrated that the expression of four V beta gene ...
Kakita, N., Kanto, T., Itose, I., Kuroda, S., Inoue, M., Matsubara, T., Higashitani, K., Miyazaki, M., Sakakibara, M., Hiramatsu, N., Takehara, T., Kasahara, A. and Hayashi, N. (2012), Comparative analyses of regulatory T cell subsets in patients with hepatocellular carcinoma: A crucial role of CD25−FOXP3− T cells. Int. J. Cancer, 131: 2573-2583. doi: 10.1002/ijc.27535 ...
Certain T cell subsets are increased in systemic sclerosis patients, particularly Vδ1|sup|+|/sup| γδ T cells in the blood and lungs and CD8|s...
Results Th17 cells were decreased in the peripheral blood of RA patients, while OA patients had surprisingly an altered ratio of CD25+Tregs and Tr1 cells in circulation. In synovial tissues Th17 cells, TFH and regulatory T cell subsets were enriched among CD4+ T cells in RA as compared to OA patients, while frequencies of Th1 cells were similar. RA patients had higher serum levels of IL-17, IL-10 and BAFF, while serum IL-21 was elevated in both RA and OA patients. Importantly, serum levels of IL-17, IL-10 and BAFF were enhanced only in patients with active RA or with detectable autoantibodies. Moreover, BAFF and IL-17 levels in synovial fluid were also higher in RA as compared to OA patients, while IL-10 concentrations were similar. B cells from synovial fluid spontaneously released high amounts of IgG in the absence of CD4+ helper T cells, and regulatory T cell subsets were unable to suppress B cell IgG production.. ...
The generation of T helper cells in vitro requires macrophages or macrophage-derived factors such as genetically related macrophage factor (GRF) or nonspecific macrophage factor (NMF). However, there is a basic difference of T helper cell induction when using particulate antigens. The present study demonstrates that this difference is based on the activation of two different T cell subsets. GRF activates short-lived T1 cells which amplify the induction of T2 cells, which are the helper cell precursors. Thus, the genetic restriction of T helper cell induction seen with soluble antigen or GRF lies on the level of macrophage or GRF interaction with T1 cells. NMF (or macrophages) and particulate antigens directly activate the helper cell precursor (T2) indicating no requirement for T1-T2 cooperation. The direct activation of the helper cell precursor with particulate antigens does not require histocompatible macrophages or NMF from histocompatible macrophages. The present results may explain some of the
Objectives Evaluate the number and distribution of circulating CD4+ T lymphocytes and their CD4+ naïve T cells (TN), central memory (TCM), non-terminated effector memory (TNTEM) and terminated effector memory (TTEM) T cells subsets in a population of recently diagnosed DMARD naive RA patients before and along the first 6 months of methotrexate (MTX) treatment. ...
Our lab studies the basic processes of T lymphocyte development and activation. We are interested in the concepts underlying lineage decisions that govern the the maturation of different T cell subsets as well as memory T cells. The information is important in understanding the balance between protective- vs. auto-immunity.. ...
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TCR gamma/delta, PerCP-eFluor™ 710, clone: eBioGL3 (GL-3, GL3), eBioscience™ 100μg; PerCP-eFluor™ 710 TCR gamma/delta, PerCP-eFluor™ 710,...
alpha beta TCR Mouse anti-Human, FITC, Clone: WT31, eBioscience™ 25 tests; FITC alpha beta TCR Mouse anti-Human, FITC, Clone: WT31, eBioscience™ Primary...
Human T cells expressing CD161 and an invariant T-cell receptor (TCR) alpha-chain (Valpha24invt T cells) specifically recognize CD1d and appear to have immunoregulatory functions. However, the physiological target cells for this T-cell population, and whether alterations in CD1d expression contribut …
The immune system has evolved to fend off challenges from a wide array of pathogens while maintaining tolerance to self-antigens and benign environmental antigens. CD4 helper T cells are critical in regulating these processes with different subsets of CD4 T cells responsible for regulating different facets of the immune system. T helper 1 (Th1) cells, which contribute to antiviral immunity, and T helper 2 (Th2) cells, which contribute to antihelminth immunity and allergy, were the first CD4 T cell subsets to be discovered. Recently a number of new subsets have been discovered. Here we review what is known about CD4 T cell subsets with particular focus on neonatal immunity. ...
Denne rapport beskriver en metode til at fremkalde kronisk eksperimentelt autoimmunt tørt øje i Lewis rotter gennem immunisering med...
Maloney began by explaining that CAR-T cells produced from distinct T-cell subsets differ in potency. NSG mice bearing Raji tumors (0.5x10 6tumor cell inoculation; day 0) were treated with human CAR-T cells manufactured from distinct T-cell subsets (on day 7). CAR-T cells produced from CD8 +T CMcells were highly potent ( Sommermeyer et al.2015).. Engineering selected T-cell subsets could enhance potency and allow delivery of the same cell product in all patients, potentially providing more uniform data on dose response and toxicity.. Pre-clinical studies have established that a defined composition of CD8 +T CMderived and CD4 +derived CAR T-cells provides optimal potency.. ...
Experimental work has shown that T cells of the immune system rapidly and specifically respond to antigenic molecules presented on the surface of antigen-presenting-cells and are able to discriminate between potential stimuli based on the kinetic parameters of the T cell receptor-antigen bond. These antigenic molecules are presented among thousands of chemically similar endogenous peptides, raising the question of how T cells can reliably make a decision to respond to certain antigens but not others within minutes of encountering an antigen presenting cell. In this theoretical study, we investigate the role of localized rebinding between a T cell receptor and an antigen. We show that by allowing the signaling state of individual receptors to persist during brief unbinding events, T cells are able to discriminate antigens based on both their unbinding and rebinding rates. We demonstrate that T cell receptor coreceptors, but not receptor clustering, are important in promoting localized rebinding, and show
يصف هذا التقرير طريقة للحث على جفاف المناعة الذاتية المزمن التجريبي العين في الفئران لويس من...
Chattopadhyay, P. K. and Roederer, M. 2005. Immunophenotyping of T Cell Subpopulations in HIV Disease. Current Protocols in Immunology. 65:12.12:12.12.1-12.12.15. ...
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Click to launch & play an online audio visual presentation by Dr. Brigitta Stockinger on CD4 T cell subsets, part of a collection of multimedia lectures.
Sigma-Aldrich offers abstracts and full-text articles by [M Lahn, H Kalataradi, P Mittelstadt, E Pflum, M Vollmer, C Cady, A Mukasa, A T Vella, D Ikle, R Harbeck, R OBrien, W Born].
Induction of IFN-γ- and TNF-α-secreting CD8+ T cell subpopulations upon DC priming.PBMC from volunteer CVD4000#65 were co-cultured with DC alone (media), or p
T cell, type of leukocyte (white blood cell) that is an essential part of the immune system. T cells are one of two primary types of lymphocytes-B cells being the second type-that determine the specificity of immune responses to antigens (foreign substances) in the body.
In order to discuss all the subsets of a given set, let us introduce the following terminology. We shall call the original set the universal set, one-element subsets will be called unit sets, and the set which contains no members the empty set. We do not introduce special names for other kinds of subsets of the universal set. As an example, let the universal set U consist of the three elements {a, b, c}. The proper subsets of U are those sets containing some but not all of the elements of U. The proper subsets consist of three two-element sets namely, {a, b}, {a, c}, and {b,... ...
Distinguishing between human lymphocyte subsets. Identification of cone cell inner and outer segments and to a lesser extent ...
el-Demiry M, James K (1988). "Lymphocyte subsets and macrophages in the male genital tract in health and disease. A monoclonal ... effects of activin and transforming growth factor beta on lymphocyte subsets in vitro". Biology of Reproduction. 58 (4): 943- ... T-lymphocytes (T-cells) are white blood cells which take part in cell-mediated immunity. They are often found within tissues ... B-lymphocytes take part in the adaptive immune response and produce antibodies. These cells are not normally found in the ...
Huang W, Berube J, McNamara M, Saksena S, O'Gorman M (2020). "Lymphocyte Subset Counts in COVID-19 Patients: A Meta-Analysis". ... revealed that another T helper subset may exist. Th9 cells are claimed to be an IL9 (interleukin 9)-producing T cell subset ... "Two subsets of memory T lymphocytes with distinct homing potentials and effector functions". Nature. 401 (6754): 708-712. doi: ... These effects are primarily due to the loss of any helper T cell that can interact with the B lymphocyte correctly. Another ...
Poussier, P., et al., Lymphopenia and abnormal lymphocyte subsets in the "BB" rat: relationship to the diabetic syndrome. ...
Harris MT, Schwarting GA, Stout RD (September 1981). "Selective expression of asialo GM1 on maturational subsets of lymphocytes ... 15 July 1997). "Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) ... in vivo differentiation into lymphocytes and potential for germ line transmission". FEBS Letters. 455 (1-2): 101-4. doi:10.1016 ...
"Transitional Type 1 and 2 B Lymphocyte Subsets Are Differentially Responsive to Antigen Receptor Signaling". J. Biol. Chem. 277 ... Chung JB, Sater RA, Fields ML, Erikson J, Monroe JG (2002). "CD23 defines two distinct subsets of immature B cells which differ ... T1 B cells are distinguished from the other subsets by the following surface marker characteristics: they are IgMhiIgD−CD21− ... 2010). "Differential expression of CD21 identifies developmentally and functionally distinct subsets of human transitional B ...
He was among those who defined the subsets of human B lymphocytes. These studies led to a redefinition of many human B cell ... "Analysis of somatic mutation in five B cell subsets of human tonsil". The Journal of Experimental Medicine. 180 (1): 329-339. ... "Receptor revision of immunoglobulin heavy chain variable region genes in normal human B lymphocytes". The Journal of ...
1996;17(2):197-9. PMID 8936281 Differences in intraepithelial lymphocyte t cell subsets isolated from murine small versus large ... his research was on experimental models of hapten-induced Inflammatory bowel disease and studying intraepithelial lymphocytes. ...
Sallusto, F.; Lenig, D.; Förster, R.; Lipp, M.; Lanzavecchia, A. (1999). "Two subsets of memory T lymphocytes with distinct ... Viola, A.; Schroeder, S.; Sakakibara, S.; Lanzavecchia, A. (1999). "T lymphocyte costimulation mediated by reorganization of ... lymphocyte activation and immunological memory and iii) human monoclonal antibodies. In 1985, using antigen-specific T and B ... and discovered a fundamental division of memory T cells into two major subsets of central memory and effector memory and ...
885-895 Han S, Dillon SR... Kelsoe G (1997). V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science. ... Kelsoe's research focuses on lymphocyte development and activation, as well as how lymphocytes specific to certain pathogens ...
"Two subsets of memory T lymphocytes with distinct homing potentials and effector functions." Nature 401.6754 (1999): 708.. ...
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (1999). "Two subsets of memory T lymphocytes with distinct homing ... These two subsets were previously called "naturally occurring" and "adaptive" (or "induced"), respectively. Both subsets ... Gamma delta T cells (γδ T cells) represent a small subset of T cells which possess a γδ TCR rather than the αβ TCR on the cell ... A T cell is a type of lymphocyte. T cells are one of the important white blood cells of the immune system and play a central ...
... s are a subset of T lymphocytes that might have some of the same functions as memory B cells. Their lineage is ... Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (October 1999). "Two subsets of memory T lymphocytes with distinct ... Those lymphocytes are capable of self-renewal as are the TCM lymphocytes and are also capable of generating both the TCM and ... TCM : TCM lymphocytes have several attributes in common with stem cells, the most important being the ability of self-renewal, ...
This selective inhibition is not observed in conventional chemotherapy which reduces the number of all lymphocyte subsets. In ...
Knockout mice that lack TOX have a severe defect in development of certain subsets of T lymphocytes. In cancer or during ... Patients with cancer typically have high levels of TOX in their tumor-infiltrating lymphocytes, and anti-tumor immunity is ... TOX is highly expressed in the thymus, the site of development of T lymphocytes. ...
"Distribution of cyclophilin B-binding sites in the subsets of human peripheral blood lymphocytes". Immunology. 91 (4): 609-17. ... This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. ... recognized by HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes which could be used as cancer vaccines, and in fact ...
Effects of Lycium barbarum polysaccharide on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing ...
Nassar GM, Montero A, Fukunaga M, Badr KF (1997). "Contrasting effects of proinflammatory and T-helper lymphocyte subset-2 ... and B-lymphocytes express ALOX5. Platelets, T cells, and erythrocytes are ALOX5-negative. In skin, Langerhans cells strongly ... "Studies on the regulation and localization of 5-lipoxygenase in human B-lymphocytes". Eur. J. Biochem. 232 (1): 37-46. doi: ...
In jawless fishes, two subsets of lymphocytes use variable lymphocyte receptors (VLRs) for antigen binding. Diversity is ... T and B lymphocytes are the cells of the adaptive immune system. The human body has about 2 trillion lymphocytes, which are 20- ... Lymphocyte receptors, Ig and TCR, are found in all jawed vertebrates. The most ancient Ig class, IgM, is membrane-bound and ... Cytotoxic T cells (also known as TC, killer T cell, or cytotoxic T-lymphocyte (CTL)) are a sub-group of T cells that induce the ...
There are no major changes in the T-lymphocytes subsets of the mesenterial lymph node. Instead γδ-T cells guide the immune ... Antigens of C. oncophora larvae and adult worms are capable of triggering lymphocyte proliferation. Moreover, excretory/ ... Primary infection does not involve recruitment of specific lymphocytes to the intestinal mucosa. ...
PTSAgs induce the VB-specific expansion of both CD4 and CD8- subsets of T Lymphocytes. TSST-1 forms homodimers in most of its ...
NK Cells are not a subset of the T lymphocyte family. Natural cytotoxicity receptors directly induce apoptosis after binding to ... granular lymphocytes known today as NK cells. The demonstration that density gradient-isolated large granular lymphocytes were ... Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. The role NK cells ... A functional marker of human non-T lymphocytes". Clinical and Experimental Immunology. 21 (2): 226-35. PMC 1538269. PMID 810282 ...
A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes". Journal of ... Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several ... "Phenotypic and functional analysis of CD4+ NKRP1A+ human T lymphocytes. Direct evidence that the NKRP1A molecule is involved in ...
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (1999). "Two subsets of memory T lymphocytes with distinct homing ... revealed that another T helper subset may exist. Th9 cells are claimed to be an IL9 (interleukin 9)-producing T cell subset ... Th17 helper cells are a subset of T helper cells developmentally distinct from Th1 and Th2 lineages producing interleukin 17 ( ... For example, the Th2 cytokine IL-10 inhibits cytokine production of both Th subsets in humans. Human IL-10 (hIL-10) suppresses ...
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (1999). "Two subsets of memory T lymphocytes with distinct homing ... A T cell, or T lymphocyte, is a type of lymphocyte (a subtype of white blood cell) that plays a central role in cell-mediated ... These two subsets were previously called "naturally occurring", and "adaptive" or "induced", respectively.[13] Both subsets ... within a population of lymphocytes known as intraepithelial lymphocytes. In rabbits, sheep, and chickens, the number of γδ T ...
2001a;97:319-327 Lymphocyte phenotype subsets in the cerebrospinal fluid of normal horses and horses with equine protozoal ...
... differential signaling requirements for activation of assembled cyclin D3-cdk4 complexes in B-1 and B-2 lymphocyte subsets". ...
Ademmer; Ebert; Müller-Ostermeyer; Friess; Büchler; Schubert; Malfertheiner (April 1998). "Effector T lymphocyte subsets in ... 2006). "Profiling lymphocyte subpopulations in peripheral blood under efalizumab treatment of psoriasis by multi epitope ligand ... "Automatic Recognition of Muscle-Invasive T-Lymphocytes Expressing Dipeptidyl-Peptidase IV (CD26) and Analysis of the Associated ... "Poisson Numbers and Poisson Distributions in Subset Surprisology". Annals of Combinatorics. 8 (4): 473-485. doi:10.1007/s00026- ...
"In Vitro Measles Virus Infection of Human Lymphocyte Subsets Demonstrates High Susceptibility and Permissiveness of both Naive ... In the later stages of infection, the virus infects other immune cell types, including B cells and T lymphocytes also via ... Three receptors for the H protein have been identified to date: complement regulatory molecule CD46, the signaling lymphocyte ...
... these specific transcription factors activate or repress target genes critical in the differentiation of the lymphocyte subsets ... Studies have confirmed the ability of some ILC subsets to convert into a different subset in the presence of specific cytokines ... There are two subsets of ILC3s, NCR- and NCR+ ILC3s, with the displayed NCR on mice ILC3s being NKp46, in comparison to NKp44 ... All ILC subsets are present in the liver and regulate the immune response to protect the tissue from viral and bacterial ...
Sallusto, F.; Lenig, D.; Förster, R.; Lipp, M.; Lanzavecchia, A. Two subsets of memory T lymphocytes with distinct homing ... T细胞(英語:T cell、T lymphocyte)是淋巴细胞的一种,在免疫反應中扮演着重要的角色。T是胸腺(thymus)而不是甲狀腺(thyroid)的英文缩写。T细胞在骨髓被製造出來之後,在胸腺内進行「新兵訓練」分化成熟為不同亚型的效应T細胞,成 ... CD4+ T cells are required for secondary expansion and memory in CD8+ T lymphocytes. Nature. February
It increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen). ... subset panel illustrates a schematic view of CD20 structure and rituximab.[35] ... including non-Hodgkin's lymphoma and lymphocyte predominant subtype, of Hodgkin's Lymphoma.[12] ...
It increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen). ... subset panel illustrates a schematic view of CD20 structure and rituximab.[36] ... "B-Lymphocyte Depletion in Patients With Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: A Randomized, Double-Blind, Placebo ... including non-Hodgkin's lymphoma and lymphocyte predominant subtype, of Hodgkin's Lymphoma.[12] This also includes ...
Determination of T-lymphocyte subsets on site in rural Tanzania: results in HIV-1 infected and non-infected individuals. ... Analysis of blood lymphocyte subsets in children living on territory that received high amounts of fallout from Chernobyl ... Correlation of CD8 lymphocyte activation with cellular viremia and plasma HIV RNA levels in asymptomatic patients infected by ... The staging and prognostic value of subset markers on CD8 cells in HIV disease. In Janossy G, Autran B. Miedema F (eds): ...
Lymphocyte. Main article: Lymphocyte. Lymphocytes are much more common in the lymphatic system than in blood. Lymphocytes are ... The complete blood cell count is a blood panel that includes the overall WBC count and various subsets such as the absolute ... Lymphocyte. 30%. Small lymphocytes 7-8. Large lymphocytes 12-15. *B cells: releases antibodies and assists activation of T ... lymphocytes) by hematopoietic lineage (cellular differentiation lineage).[6] Lymphocytes can be further classified as T cells, ...
Another example occurs in activated T cell lymphocytes, i.e., when a T cell is induced to mature by binding to a peptide:MHC ... FGF9 and their receptors encompass a growth factor autocrine loop that is active in a subset of gefitinib-resistant NSCLC cell ... "Neuregulin-1-Mediated Autocrine Signaling Underlies Sensitivity to HER2 Kinase Inhibitors in a Subset of Human Cancers". Cancer ...
However, the various subsets may also be considered part of the innate immune system where a restricted TCR or NK receptors may ... One can see red blood cells, several knobby white blood cells including lymphocytes, a monocyte, a neutrophil, and many small ... Like other 'unconventional' T cell subsets bearing invariant T cell receptors (TCRs), such as CD1d-restricted Natural Killer T ... Invertebrates do not possess lymphocytes or an antibody-based humoral immune system, and it is likely that a multicomponent, ...
Yamada H, Yamada K, Waki M, Umegaki K (October 2004). "Lymphocyte and plasma vitamin C levels in type 2 diabetic patients with ... Another subset of trials looked at therapeutic use, meaning that vitamin C was not started unless the people started to feel ... Emadi-Konjin P, Verjee Z, Levin AV, Adeli K (May 2005). "Measurement of intracellular vitamin C levels in human lymphocytes by ... A subset of trials reported that supplementation reduced the incidence of colds by half in marathon runners, skiers, or ...
For virus-associated tumors, such as cervical cancer and a subset of head and neck cancers, epitopes derived from viral open ... They become activated and start to secrete cytokines, substances that activate cytotoxic T lymphocytes (CTL), antibody- ... Cytotoxic T lymphocytes that recognize these antigens may be able to destroy tumor cells.[15] ... to assess reactivity in patients treated by either tumor-infiltrating lymphocyte (TIL) cell therapy or checkpoint blockade. ...
PrP immune cells include hematopoietic stem cells, mature lymphoid and myeloid compartments, and certain lymphocytes; also, it ... attenuated the epilepsy-induced death phenotype seen in a subset of these animals.[54] Taken collectively, recent evidence ...
... causes a response in a type of immune cell called a TH2 lymphocyte, which belongs to a subset of T cells that produce a ... lymphocytes, eosinophils, and macrophages to the initial site. The reaction is usually seen 2-24 hours after the original ... cytokine called interleukin-4 (IL-4). These TH2 cells interact with other lymphocytes called B cells, whose role is the ...
... s are a subset of cytokines that are produced by a type of immune cell known as a lymphocyte.[1] They are protein ... Circulating lymphocytes can detect a very small concentration of lymphokine and then move up the concentration gradient towards ... Lymphokines have many roles, including the attraction of other immune cells, including macrophages and other lymphocytes, to an ...
Many cells of the immune system are required for this process, including lymphocytes (T-cells and B-cells) and antigen ... These antibodies can be subdivided according to their specificity, and each subset has different propensities for specific ... In normal physiology, lymphocytes that recognise human proteins (autoantigens) either undergo programmed cell death (apoptosis ... This self-tolerance means that lymphocytes should not incite an immune response against human cellular antigens. Sometimes, ...
The rare disorder X-linked neutropenia has also been linked to a specific subset of WAS mutations.[9] ... and genomic instability in lymphocytes". The Journal of Experimental Medicine. 207 (6): 1145-52. doi:10.1084/jem.20091245. PMC ...
... discs large tumor suppressor binds to p56lck tyrosine kinase and Shaker type Kv1.3 potassium channel in T lymphocytes". The ... "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway". The ...
Nassar GM, Montero A, Fukunaga M, Badr KF (1997). "Contrasting effects of proinflammatory and T-helper lymphocyte subset-2 ... and B-lymphocytes express ALOX5. Platelets, T cells, and erythrocytes are ALOX5-negative. In skin, Langerhans cells strongly ... "Studies on the regulation and localization of 5-lipoxygenase in human B-lymphocytes". Eur. J. Biochem. 232 (1): 37-46. doi: ...
Specific acquired immunity against infectious diseases may be mediated by antibodies and/or T lymphocytes. Immunity mediated by ... a contagious disease is a subset of infectious disease that is especially infective or easily transmitted. Other types of ... or by T lymphocytes, which will kill a cell parasitized by a microorganism. ...
Histologic findings of a deep mixed infiltrate with lymphocytes, plasma cells, and neutrophils (with or without eosinophils) ... found an association between a polymorphism in the TNF-alpha gene and risk for oral LP in a subset of patients. An Italian ...
IELs: intraepithelial lymphocytes). *^ a b Lionetti E, Francavilla R, Pavone P, Pavone L, Francavilla T, Pulvirenti A, Giugno R ... "In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of ... Therefore, these forms of the receptor are more likely to activate T lymphocytes and initiate the autoimmune process.[28] ... Biopsy of small bowel showing coeliac disease manifested by blunting of villi, crypt hypertrophy, and lymphocyte infiltration ...
Dermatographism is the most common form of a subset of chronic hives, acknowledged as "physical hives".[citation needed] ... Hydroxychloroquine is an antimalarial agent that suppresses T lymphocytes. It has a low cost however it takes longer than ...
In jawless fishes, two subsets of lymphocytes use variable lymphocyte receptors (VLRs) for antigen binding.[33] Diversity is ... LymphocytesEdit. Main article: Lymphocyte. T and B lymphocytes are the cells of the adaptive immune system. The human body has ... CD8+ T lymphocytes and cytotoxicityEdit. Main article: Cytotoxic T cell. Cytotoxic T cells (also known as TC, killer T cell, or ... The cells that carry out the adaptive immune response are white blood cells known as lymphocytes. B cells and T cells, two ...
lymphocyte migration. • T cell migration. • thymocyte migration. • DN2 thymocyte differentiation. • DN3 thymocyte ... NKT cell subsets with distinct cytokine-producing capacity". Blood. 100 (1): 11-6. doi:10.1182/blood-2001-12-0196. PMID ... 1997). "Identification of CCR6, the specific receptor for a novel lymphocyte-directed CC chemokine LARC". J. Biol. Chem. 272 ( ... "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes". J. Immunol. 168 (1): 65-72 ...
Phenotypically distinct subsets of CD4+ T cells induce or protect from chronic intestinal inflammation in C. B-17 scid mice. „ ... A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. „J Exp Med". 144 ... I. Evidence for the active participation of T cells in natural self-tolerance; deficit of a T cell subset as a possible cause ... OX-22high CD4+ T cells induce wasting disease with multiple organ pathology: prevention by the OX-22low subset. „J Exp Med". ...
The approval is for a subset of kidney cancer patients who are at intermediate risk for disease recurrence. It awaits approval ... Shankaran V, Ikeda H, Bruce AT, White JM, Swanson PE, Old LJ, Schreiber RD (2001-04-26). "IFNgamma and lymphocytes prevent ...
Hence one could choose any subset of the equations, with the number equal to the number of variables, and solve them. But, when ... Low persistence of micronucleus frequency in lymphocytes of individuals after cardiac scan". Mutat. Res. Fundam. Mol. Mech. ... a classifier or more basic algorithm is trained to distinguish trials for different conditions within a subset of the data. The ... "Genotoxic effects of 3 T magnetic resonance imaging in cultured human lymphocytes". Bioelectromagnetics. 32 (7): 535-42. doi: ...
"Lymphocyte activation gene-3 expression defines a discrete subset of HIV-specific CD8+ T cells that is associated with lower ... "Entrez Gene: LAG3 lymphocyte-activation gene 3".. *^ a b c Triebel F, Jitsukawa S, Baixeras E, Roman-Roman S, Genevee C, Viegas ... Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene.[5] LAG3, which was ... "T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of ...
Janeway CA Jr, Travers P, Walport M, et al., Generation of lymphocytes in bone marrow and thymus, "Immunobiology: The Immune ... immunohistochemical characterization of four thymic epithelial subsets defined by monoclonal anti-keratin antibodies., Eur J ... Janeway CA Jr, Travers P, Walport M, et al., Generation of lymphocytes in bone marrow and thymus, "Immunobiology: The Immune ... P.G. Isaacson, A.J. Norton, B.J. Addis, THE HUMAN THYMUS CONTAINS A NOVEL POPULATION OF B LYMPHOCYTES, The Lancet, 330. ...
"Lymphocyte subsets in term and significantly preterm UK infants in the first year of life analysed by single platform flow ... Lymphocyte development, veebiversioon (vaadatud 27.02.2014) (inglise keeles). *The Development and Survival of Lymphocytes, ... Alberts B, Johnson A, Lewis J, et al., Lymphocytes and the Cellular Basis of Adaptive Immunity, Molecular Biology of the Cell, ... Janeway CA Jr, Travers P, Walport M, et al., Generation of lymphocytes in bone marrow and thymus, "Immunobiology: The Immune ...
... including mesenchymal stem cells and regulatory T-lymphocytes. The mesenchymal stem cells thus alter the outcome of the immune ... response by changing the cytokine secretion of dendritic and T-cell subsets. This results in a shift from a pro-inflammatory ...
T-lymphocyte subsets in acute illness.. Feeney C1, Bryzman S, Kong L, Brazil H, Deutsch R, Fritz LC. ... but no relationship was found between total lymphocyte or lymphocyte subset counts and APACHE II score, predicted mortality ... To determine the range of T-lymphocyte subsets (CD4, CD8, and CD4/CD8 ratios) in acutely ill, hospitalized patients and to ... predicted mortality rate with T-lymphocyte subsets.. SETTING: Urban county hospital intensive care unit (ICU), serving as the ...
... Tim R Mosmann, University of Rochester Medical Center, Rochester, New York, USA Jonathan ... 1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401 (6754): 708-712. ... Mosmann, Tim R, and Rebhahn, Jonathan A(Nov 2016) T Lymphocytes: Plasticity of Subsets. In: eLS. John Wiley & Sons Ltd, ... 1990) Evidence for compartmentalization of functional subsets of CD2+ T lymphocytes in atopic patients. Journal of Immunology ...
T-cell subsets in the peripheral blood of 63 primary lung cancer patients (23 with adenocarcinoma, 23 with squamous cell ... Correlations between T-lymphocyte subset values and stages or cell types of disease were sought. Total lymphocytes in the ... T-cell subsets in the peripheral blood of 63 primary lung cancer patients (23 with adenocarcinoma, 23 with squamous cell ... Previous Document: Lysis of autologous tumor cells by peripheral blood lymphocytes treated with interleukin 2 in patien.... ...
To test our hypothesis that these changes were due to oestrogen deficiency we studied lymphocyte subsets in patients with ... shown that in patients with idiopathic premature ovarian failure there were significant changes in lymphocyte subsets. ... Blood was taken for serum oestradiol, lymphocyte counts and lymphocyte subset counts (CD2+, CD4+, CD8+ and B cells) before ... Lymphocyte subsets in patients with oestrogen deficiency J Reprod Immunol. 1991 May;20(1):85-91. doi: 10.1016/0165-0378(91) ...
Reference values of lymphocyte subsets in healthy, HIV-negative children in Cameroon.. Sagnia B1, Ateba Ndongo F, Ndiang Moyo ... Normal lymphocyte subsets values among children from Cameroon differ from reported values in Caucasian and some African ... Lymphocyte subset reference values used to monitor infectious diseases, including HIV/AIDS, tuberculosis, malaria, or other ... We evaluated about 23 different lymphocyte subsets in which the absolute number and percentage values differed significantly (P ...
Differential function of intestinal intraepithelial lymphocyte subsets.. T A Barrett, T F Gajewski, D Danielpour, E B Chang, K ... Differential function of intestinal intraepithelial lymphocyte subsets.. T A Barrett, T F Gajewski, D Danielpour, E B Chang, K ... Differential function of intestinal intraepithelial lymphocyte subsets.. T A Barrett, T F Gajewski, D Danielpour, E B Chang, K ... Differential function of intestinal intraepithelial lymphocyte subsets. Message Subject (Your Name) has forwarded a page to you ...
... Jue Lin,1 Joshua Cheon,1 Rashida Brown,2 ...
Lymphocytes and their subsets, NK cells were signed and measured by flow cytometric immunophenotyping in Coulter EPIX XL with ... In the course of active infection B.burgdorferi essential changes in lymphocytes subsets are observed. Decreased percentages of ... subsets CD4, CD8, NK cells, activated lymphocytes with fenotype CD3+HLA-DR, CD4/CD8 rate in Lyme borreliosis was carried out ... Percentage CD4 in II examination (x = 42) was lower than control (x = 45.8). Subset of CD8 had lower value in I examination (x ...
Dumitriu I.E., Kaski J.C. (2013) The Role of Lymphocytes in the Pathogenesis of Atherosclerosis: Focus on CD4+ T Cell Subsets. ... The Role of Lymphocytes in the Pathogenesis of Atherosclerosis: Focus on CD4+ T Cell Subsets. ... Functional subsets of CD4 T cells in rheumatoid synovitis. Arthritis Rheum. 1998;41(12):2108-16.PubMedCrossRefGoogle Scholar ... Acute Coronary Syndrome Th17 Cell Atherosclerotic Plaque Treg Cell Cell Subset These keywords were added by machine and not by ...
T Lymphocyte Subsets in Patients with Bronchiectasis B.C. Leahy B.C. Leahy ... B.C. Leahy, M.A. Horan, T.B. Stretton; T Lymphocyte Subsets in Patients with Bronchiectasis. Clin Sci (Lond) 1 February 1984; ...
T-Lymphocyte Subsets in Adult Coeliac Disease G. R. Corazza; G. R. Corazza ... G. R. Corazza, P. Tabacchi, M. Frisoni, M. Londei, D. Bastia, G. Gasbarrini; T-Lymphocyte Subsets in Adult Coeliac Disease. ...
Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells ... TI - Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B ... Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells ... Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells ...
Ana A. Weil, Mohammad Arifuzzaman, Taufiqur R. Bhuiyan, Regina C. LaRocque, Aaron M. Harris, Emily A. Kendall, Azim Hossain, Abdullah A. Tarique, Alaullah Sheikh, Fahima Chowdhury, Ashraful I. Khan, Farhan Murshed, Kenneth C. Parker, Kalyan K. Banerjee, Edward T. Ryan, Jason B. Harris, Firdausi Qadri, Stephen B. Calderwood ...
Monitoring lymphocyte subsets, especially CD4+T cells, may be helpful for identifying the presence of infection in SLE patients ... Association between the lymphocyte subsets and infections was further analyzed. SLE patients with infections (n=13) showed a ... Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B ... LU, Zhimin et al. Altered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections. ...
Distribution of lymphocyte subsets. The lymphocyte subset results of these three groups including HC, non-infection SLE ... The percentages and counts of the lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+ ... Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B ... Monitoring lymphocyte subsets, especially CD4+T cells, may be helpful for identifying the presence of infection in SLE patients ...
This study investigated the effects of a temporally confined naturalistic stressor (academic stress) on immune functions. Furthermore, moderating influences of a number of psychological variables were assessed. Five blood samples were obtained from 20 students during an observation period of 8 weeks, starting 4.5 weeks before an exam period up to 1 week following the last exam. The analysis of 45 immune parameters revealed several time-dependent changes attributable to examination stress. We observed a reduction in the absolute numbers of natural killer (NK) cells and monocytes in peripheral blood and a shift towards more immature and naïve cells within NK and T cell populations. In addition, IL-6 and TNF-α production by LPS-stimulated monocytes was increased. Psychological variables were grouped by means of factor analyses into two factors. One factor, which was interpreted as an indication of chronic stress, moderated the relationships between academic stress and percentages of mature CD57+ NK cells
Hepatitis C virus (HCV) in lymphocyte subsets and in B lymphocytes expressing rheumatoid factor cross-reacting idiotype in type ... in lymphocyte subsets and in B lymphocytes expressing rheumatoid factor cross-reacting idiotype in type II mixed ... B-lymphocytes are predominantly involved in viral propagation of hepatitis C virus (HCV). Arch Virol (Suppl) 1994; 9: 307-16.. ... Evidence of selective expansion of a B cell subset characterized by the expression of cross-reactive idiotypes. Arthritis Rheum ...
This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. These results can be used for other ... Moroccan lymphocyte subsets reference ranges: age, gender, ethnicity, and socio-economic factors dependent differences.. *. ... This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. These results can be used for other ...
Lymphocyte subsets, phytohaemagglutinin responsiveness of blood lymphocytes, and interleukin 2 production in sarcoidosis. ... Lymphocyte subsets, phytohaemagglutinin responsiveness of blood lymphocytes, and interleukin 2 production in sarcoidosis. ...
... ... Kj Blank, Rd Mulero-Marchese, and Tg Sieck, "Strain-dependent migration of lymphocytes to the vaginal mucosa after peripheral ...
These molecules are essential markers for the identification and isolation of leukocytes and lymphocyte subsets. Here, we ... Using the resource, we examined differentially expressed proteins between granulocyte, monocyte and dendritic cell subsets, and ... Using the resource, we examined differentially expressed proteins between granulocyte, monocyte, and dendritic cell subsets, ... and percentage of positivity for all markers on all subsets and was developed into an interactive CD Maps web resource. ...
The present study aimed to evaluate the distribution of major lymphocyte subsets and cytokine expression profiles in the ... blood lymphocyte subpopulations, and serum cytokines, respectively. The FA-exposed workers were divided into low and high ... Though the mechanisms by which FA affects blood lymphocytes and/or lymphocyte subsets are still unknown, the results from ... Major lymphocyte subsets were analyzed on the same day. Serum was separated within 4 h after collection, put into 1.5 mL tubes ...
Secondary: To compare, the absolute numbers and percentages of lymphocytes subsets as measured in the Sysmex-XN 20 analyser to ... An Observational Study Exploring the Utility of the Sysmex-XN 20 Analyser to Assess Lymphocyte Subsets and Other Haematological ... Sysmex-XN 20 Analyser to Assess Lymphocyte Subsets and Other Haematological Parameters in Chronic/Acute Viral Infections (SASA) ... Sysmex-XN 20 Analyser to Assess Lymphocyte Subsets and Other Haematological Parameters in Chronic/Acute Viral Infections ...
Transmigration of lymphocyte subsets across these barriers and their activation/differentiation within the periphery and ... and enables simulation-based prediction of distribution and differentiation stages of lymphocyte subsets in the case of limited ... correctly reproduces acquired data on location and differentiation states of distinct lymphocyte subsets under homeostatic and ... are warranted that qualitatively and quantitatively predict the distribution and differentiation stages of lymphocyte subsets ...
Alcohol use is known to alter immune function and has immunosuppressive effects that may modify T-lymphocyte subpopulations. ... Crum, R., Galai, N., Cohn, S., Celentano, D., & Vlahov, D. (1996). Alcohol Use and T-Lymphocyte Subsets among Injection Drug ... Alcohol Use and T-Lymphocyte Subsets among Injection Drug Users with HIV-1 Infection: A Prospective Analysis. ... Alcohol Use and T-Lymphocyte Subsets among Injection Drug Users with HIV-1 Infection: A Prospective Analysis ...
This study aimed to investigate lymphocyte populations in non-diabetic patients with early clinical presentation of coronary ... T-lymphocytes), CD19(+) (B-lymphocytes), CD4(+) (helper/inducer lymphocytes) and CD8(+) (suppressor/cytotoxic lymphocytes) ... This study aimed to investigate lymphocyte populations in non-diabetic patients with early clinical presentation of coronary ...
Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression.. A J Schlueter, T R Malek, C ... Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. ... Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. ... Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. ...
Lymphocyte subsets and ocular inflammation: future prospects for immune deviation therapy?. British Journal of Ophthalmology ...
Peripheral blood lymphocytes study. Lymphocyte subsets were studied by three-color flow cytometry in whole blood after staining ... The present study confirms that endometrium presents a wide spectrum of T and NK lymphocyte subsets. Endometrial T lymphocytes ... In this study we aimed to make the first step to compare the levels of T and NK lymphocyte subsets in endometrium and ... Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood. Viktor P. Chernyshov ...
  • We mainly investigated the major lymphocyte subsets in untreated SLE patients and analyzed their relation to infections. (scielo.br)
  • The present study aimed to evaluate the distribution of major lymphocyte subsets and cytokine expression profiles in the peripheral blood of FA-exposed workers. (plos.org)
  • Results: The total lymphocyte count (p=0.00044) and three of the four major lymphocyte subsets (ie, CD4+ T cells (p=0.00019), CD8+ T cells (p=0.0058) and B cells (p=0.017)) were higher in exposed versus control workers and findings were highly consistent when stratified by smoking status. (elsevier.com)
  • These results showed that early-life environmental PCB exposure was associated with fluctuations in major lymphocyte subsets in children, suggesting that there is a post-natal immune system response to PCB exposures. (elsevier.com)
  • Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. (muni.cz)
  • High performance liquid chromatography, flow cytometry, and cytometric bead array were used to analyze FA in air sample and formic acid in urine, blood lymphocyte subpopulations, and serum cytokines, respectively. (plos.org)
  • Objectives: Alcohol use is known to alter immune function and has immunosuppressive effects that may modify T-lymphocyte subpopulations. (rti.org)
  • If evidence for such an association could be found, it would suggest a direct or indirect (via DC) immunoregulatory conversation of these two lymphocyte subpopulations. (harvest-re.com)
  • We used flow cytometry to investigate modifications of lymphocyte subpopulations related to pulmonary cytomegalovirus infections in blood and BAL samples from a series of 13 lung transplant recipients. (biomedcentral.com)
  • Our results demonstrate that pulmonary CMV infection is associated with a significant increase in the total lymphocyte population in BAL samples, but with minor modifications of the various lymphocyte subpopulations and a significantly higher absolute number of B lymphocytes in blood samples. (biomedcentral.com)
  • Cytomegalovirus pulmonary infection is accompanied by only minor changes in BAL lymphocyte subpopulations. (biomedcentral.com)
  • The study of BAL lymphocyte subpopulations therefore appears to be of limited clinical value in the diagnosis of pulmonary CMV infection. (biomedcentral.com)
  • The numbers and the distribution of T-lymphocyte subpopulations in lymph node granulomas from 11 patients with sarcoidosis were studied in cryostat sections by an immunoperoxidase technique. (semanticscholar.org)
  • Peripheral blood lymphocyte subpopulations and self-reported stress (Perceived Stress Scale) were measured before training and 1-2 months later as exams approached. (isharonline.org)
  • These results demonstrate that there is significant abnormality in the subpopulations of lymphocytes in patients with sudden hearing loss, suggesting the immune system as a possible etiopathogenic factor in this entity. (kjorl.org)
  • Lymphocyte subpopulations were quantified by immunofluorescence using monoclonal antibodies against total T-cells, T-helper and T-suppressor cells. (medicdebate.org)
  • Each of these lymphocyte subpopulations have distinct effector and regulatory functions and are maintained in homeostasis under normal physiological conditions. (testcatalog.org)
  • Acute illness alone, in the absence of HIV infection, can be associated with profound decreases of T-lymphocyte populations. (nih.gov)
  • In the course of active infection B.burgdorferi essential changes in lymphocytes subsets are observed. (prohealth.com)
  • Monitoring lymphocyte subsets, especially CD4+T cells, may be helpful for identifying the presence of infection in SLE patients. (scielo.br)
  • As we know, peripheral lymphocytes play an important role in anti-infection immune response and abnormal status accounts for a bad ending in infection diseases ( 7 ). (scielo.br)
  • Methods: Using prospective data from a cohort of IDUs in a study of the natural history of HIV infection, we examined the relationship of alcohol use and changes in T-lymphocyte subsets. (rti.org)
  • Measles virus (MV) infection of immune cells is mediated by the cellular receptor CD150, expressed by subsets of lymphocytes, dendritic cells, macrophages, and thymocytes. (asm.org)
  • However, limited information is available about the CD150 expression and relative susceptibility to MV infection of B-cell subsets. (asm.org)
  • In this study, we assessed the susceptibility and permissiveness of naive and memory T- and B-cell subsets from human peripheral blood or tonsils to in vitro MV infection. (asm.org)
  • We demonstrated that T H 1T H 17 cells and plasma and germinal center B cells were the subsets most susceptible and permissive to MV infection. (asm.org)
  • Our study suggests that both naive and memory B cells, along with several other antigen-experienced lymphocytes, are important target cells of MV infection. (asm.org)
  • Proviral burden and infection kinetics of feline immunodeficiency virus in lymphocyte subsets of blood and lymph node. (asm.org)
  • Peripheral blood lymphocyte, NK cell and activation markers were detected by flow cytometry in renal allograft recipients with (TpC+) or without (TpC-) HCV infection, and compared with age- and sex-matched patients with post-transfusional chronic HCV infection (TfC+) and healthy controls. (hku.hk)
  • Peripheral blood suppressor/cytotoxic T lymphocytes are increased, whereas activated helper/inducer T lymphocytes and NK cells are reduced, in renal allograft recipients with HCV infection. (hku.hk)
  • The aim of this study was to analyze the changes seen in lymphocyte subsets and immunomodulatory cytokine expression in pigs after an acute experimental infection with a European PRRSV field isolate. (surrey.ac.uk)
  • However, increased blood B-lymphocytes seems to be a clinical feature associated with CMV infection. (biomedcentral.com)
  • All patients transplanted in our institution are routinely evaluated by BAL for detection of opportunistic infection and routine analysis of the various subsets of intra-alveolar cells, particularly lymphocytes. (biomedcentral.com)
  • The most commonly used hematology tests to monitor HIV infection are the complete blood count (CBC) and lymphocyte subsets. (thebody.com)
  • Lymphocytes are the key WBCs involved in immune responses (see CD4 count below) and are often lowered by HIV infection. (thebody.com)
  • 0.0001, respectively) and lymphocyte subsets (T cells, CD4 + and CD8 + T cell subsets) of severe COVID-19 and severe influenza A patients to be significantly lower than those of healthy donors at early infection stages. (researchsquare.com)
  • To determine the range of T-lymphocyte subsets (CD4, CD8, and CD4/CD8 ratios) in acutely ill, hospitalized patients and to determine whether these concentrations correlate with illness severity, survival rate, or immunodepression. (nih.gov)
  • Blinded human immunodeficiency virus (HIV) and lymphocyte testing was performed on samples from all patients on ICU admission. (nih.gov)
  • T-cell subsets in the peripheral blood of 63 primary lung cancer patients (23 with adenocarcinoma, 23 with squamous cell carcinoma and 17 with small cell carcinoma) and 24 normal healthy controls were determined by indirect immunofluorescence, using the monoclonal antibody reagents OKT3, OKT4 and OKT8. (biomedsearch.com)
  • We have previously shown that in patients with idiopathic premature ovarian failure there were significant changes in lymphocyte subsets. (nih.gov)
  • To test our hypothesis that these changes were due to oestrogen deficiency we studied lymphocyte subsets in patients with oestrogen deficiency due to other causes. (nih.gov)
  • Blood was taken for serum oestradiol, lymphocyte counts and lymphocyte subset counts (CD2+, CD4+, CD8+ and B cells) before oestrogen replacement in 19 patients with gonadal dysgenesis, 22 patients with hypothalamic-pituitary failure and 24 healthy female control subjects. (nih.gov)
  • The CD4:CD8 ratio in both groups of patients was significantly lower than that in the normal control subjects while the percentages and counts of lymphocytes and CD8+ cells were significantly higher. (nih.gov)
  • Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression. (muni.cz)
  • N2 - Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. (muni.cz)
  • We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. (muni.cz)
  • The B cell subsets showed a markedly increased proliferation in CVID 21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. (muni.cz)
  • FO I subset is significantly underrepresented in CVID 21lo patients. (muni.cz)
  • A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression. (muni.cz)
  • The objective of this study was to evaluate the distribution of lymphocyte subsets in untreated SLE patients with infections. (scielo.br)
  • Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+CD56NK cells, and CD3+CD16+CD56NKT cells in 25 healthy controls and 52 treatment-naive SLE patients, among whom 13 were complicated with infections. (scielo.br)
  • Lymphocyte subset characterization in patients with early clinical presentation of coronary heart disease. (biomedsearch.com)
  • This study aimed to investigate lymphocyte populations in non-diabetic patients with early clinical presentation of coronary heart disease (CHD). (biomedsearch.com)
  • Study Objective: To observe the effect of dexmedetomidine (DEX) on T-lymphocyte subsets and natural killer (NK) cells in the peripheral blood of perioperative patients with colorectal cancer. (scirp.org)
  • T. Zhao, Z. Liu, A. Yu and Z. Zhang, "Effects of Intraoperative Administration of Dexmedetomidine on the Percentage of T-Lymphocyte Subsets and Natural Killer Cells in Patients with Colorectal Cancer," Open Journal of Anesthesiology , Vol. 3 No. 2, 2013, pp. 104-108. (scirp.org)
  • In patients with recurrent miscarriages, no correlation was found between peripheral and uterine NK cells but an association between peripheral lymphocytes (CD45+CD3-DR+ and CD45+CD3+CD8+DR+) and uterine NK cells was confirmed and it was supposed that T, B and NK lymphocytes provided cytokines for the differentiation of uterus NK cells [8]. (termedia.pl)
  • To evaluate the expression of tumor PD-L1 and changes in tumor-infiltrating lymphocyte (TIL) populations in patients with metastatic melanoma treated with targeted MAPK inhibitors. (aacrjournals.org)
  • BRAFi-treated patients showed significant increases in CD4 + , CD8 + , and PD-1 + lymphocytes from PRE to EDT ( P = 0.001, P = 0.001, P = 0.017, respectively), and Combi-treated patients showed similar increases in CD4 + and CD8 + lymphocytes from PRE to EDT ( P = 0.017, P = 0.021). (aacrjournals.org)
  • Objectives Evaluate the number and distribution of circulating CD4+ T lymphocytes and their CD4+ naïve T cells (T N ), central memory (T CM ), non-terminated effector memory (T NTEM ) and terminated effector memory (T TEM ) T cells subsets in a population of recently diagnosed DMARD naive RA patients before and along the first 6 months of methotrexate (MTX) treatment. (bmj.com)
  • Methods The number of circulating CD4+ T lymphocytes, and of their T N , TM, TCM and TEM subsets in fifty untreated patients with RA before MTX treatment and at 3 and 6 months of treatment were assayed using a multiparametric flow cytometry. (bmj.com)
  • MTX non responder naïve RA patients show from basal conditions a significative expansion of CD4+ T NTEM lymphocytes and develop significant expansion of CD4+ T CM , and CD4+ T EM lymphocyte subsets along MTX treatment. (bmj.com)
  • Conclusions Recently diagnosed DMARD naive RA patients show involvement of the circulating CD4+ T lymphocytes activation/differentiation stage subsets. (bmj.com)
  • MTX treatment show immunomodulatory effects on CD4+ T lymphocyte compartment with different behavior in responder and non-responder patients. (bmj.com)
  • To investigate the change of T-lymphocyte subsets in peripheral blood of rheumatoid arthritis (RA) patients and analyze the effects of Fuzheng Qubi Decoction (FZQBD) on T-cell subsets. (springer.com)
  • Abnormal cellular immunity exists in RA patients, FZQBD could adjust the abnormal T-lymphocyte subsets to normalize it. (springer.com)
  • TfC+ patients and controls had similar numbers and proportions for the lymphocyte subsets and NK cells. (hku.hk)
  • In the present study, we looked for a possible association of NK cells with certain Treg subsets in patients with good long\term renal allograft acceptance. (harvest-re.com)
  • We therefore speculate that whereas healthy controls have stable NK cells counts, NK cell and Treg counts increase with time post\transplant in patients with good graft function and direct or indirect (via DC) conversation of these cell subsets may prevent graft damage by the innate immune system. (harvest-re.com)
  • In this study, we thus investigated the expression of infiltrating lymphocyte subsets (CD3, CD20, CD4, and CD8) and T helper (Th) cytokines including interferon (IFN)-γ (Th1 type), interleukin (IL)-4 (Th2 type), IL-17 (Th17 type), and IL-10 (regulatory T cell type) by immunohistochemistry in buccal mucosa specimens from 2 GS patients compared with 15 OLP patients. (nih.gov)
  • IFN-γ and IL-10 were strongly detected in the infiltrating lymphocytes of all patients. (nih.gov)
  • Lymphocyte subsets in the buccal mucosa from patients with GS and OLP. (nih.gov)
  • Since the block of lymphocyte egress is not complete, we analyzed subset composition and migratory responsiveness towards S1P of the lymphocytes remaining in the circulation of MS patients treated with fingolimod or IFN-β. (biomedcentral.com)
  • Here we show that fingolimod treatment increased the responsiveness of the remaining T-lymphocytes towards S1P and that B cell migration of IFN-β treated patients changed from weak to normal. (biomedcentral.com)
  • Altered cellular immune function has been demonstrated in patients with lung cancer, including decreased numbers of circulating lymphocytes and changes in the percentage of lymphocytes in various functional subsets. (elsevier.com)
  • We quantitated lymphocyte subsets in 54 patients with lung cancer including patients with limited (stages 1 and 2) nonsmall cell lung cancer (NSCLC, n = 23), advanced (stage 3) NSCLC (n = 16), and small cell cancer (SCLC, n = 15). (elsevier.com)
  • Serum albumin was decreased in 15 lung cancer patients, and lymphocyte subsets were separately evaluated in these patients. (elsevier.com)
  • Lymphocyte populations in cancer patients were compared to those of nonsmokers and a smoking patient population. (elsevier.com)
  • A decrease in population of T-lymphocytes subsets is frequent in patients with SCLC, advanced NSCLC, and lung cancer patients with hypoalbuminemia. (elsevier.com)
  • Herein, we investigated lymphocyte subsets in patients with two major subtypes of GBS (acute inflammatory demyelinating polyneuropathy, AIDP, and acute motor axonal neuropathy, AMAN) before and after treatment with IVIG, and explored the possible mechanism of IVIG action. (biomedcentral.com)
  • We analyzed by flow cytometry the different lymphocytes subsets in the peripheral blood of patients affected by IgM-related neuropathy, chronic inflammatory demyelinating polyneuropathy (CIDP), monoclonal gammopathy of undetermined significance and healthy subjects, to investigate whether different immunological patterns may differentiate the classical phenotype from atypical forms. (unicatt.it)
  • IFN-gamma producing CD4+ and CD8+ T lymphocytes, as well as CD4+ and CD8+ T cells expressing T-bet (T-helper type 1, Th1) were increased in CIDP patients. (unicatt.it)
  • We did not find any significant differences in the different lymphocytes subsets in the IgM-related neuropathy between patients with classical and atypical phenotype. (unicatt.it)
  • For patients with PTLD refractory to Rituximab, donor lymphocyte infusion (DLI) is established as a successful option for salvage therapy. (frontiersin.org)
  • T-cell subsets were measured in the peripheral blood of 33 patients with heart failure from idiopathic dilated cardiomyopathy, 22 patients with heart failure from other causes, and 33 normal controls. (elsevier.com)
  • We performed network meta-analyses to determine differences in blood cells, lymphocyte subsets, and cytokines in COVID-19 patients with different clinical stages. (biomedcentral.com)
  • Seventy-one studies were included involving 8647 COVID-19 patients, White blood cell (WBC), neutrophil (NEUT), IL-6, and IL-10 counts increased significantly with worsening of the COVID-19, while lymphocyte (LYM) counts decreased. (biomedcentral.com)
  • By retrospectively reviewing of patients in four groups (healthy controls, severe influenza A, non-severe COVID-19 and severe COVID-19) who were admitted to Ditan hospital between 2018 to 2020, we performed flow cytometric analysis and compared the absolute counts of leukocytes, lymphocytes, and lymphocyte subsets of the patients at different time points (weeks 1- 4). (researchsquare.com)
  • Our study suggests the plausible role of lymphocyte subsets in disease progression, which in turn affects prognosis and recovery duration in patients with severe COVID-19 and influenza A. (researchsquare.com)
  • This study was performed to define the alteration of peripheral blood T lymphocyte subsets in a group of patients with sudden deafness. (kjorl.org)
  • Influence of fingolimod on basic lymphocyte subsets frequencies in the peripheral blood of multiple sclerosis patients - preliminary study. (blogspot.com)
  • Fingolimod is a drug administered orally to adult patients treated for relapsing remitting course of multiple sclerosis (MS). Mode of action of fingolimod is based on intense S1P1 receptor stimulation and "arresting" lymphocytes in lymphatic organs. (blogspot.com)
  • Objective of the research was to assess changes in the frequencies of basic lymphocyte subsets in patients treated for multiple sclerosis with the use of fingolimod. (blogspot.com)
  • Lymphocyte subsets in patients with lung cancer treated with thiophosphoric acid alkaloid derivatives from Chelidonium majus L. (Ukrain). (medicdebate.org)
  • When compared with normal subjects, absolute numbers of T lymphocytes were reduced in patients with active ulcerative colitis or Crohn's disease (p less than 0.05). (ox.ac.uk)
  • 1 month) underwent venous blood sampling in order to determinate CD3(+) (T-lymphocytes), CD19(+) (B-lymphocytes), CD4(+) (helper/inducer lymphocytes) and CD8(+) (suppressor/cytotoxic lymphocytes) populations. (biomedsearch.com)
  • One might speculate that graft\specific CD8+ lymphocytes were killed by cytotoxic Treg, thereby preventing increases of CD8+ effector cells and keeping post\transplant CD8+ lymphocyte counts at a stable level. (harvest-re.com)
  • In murine models of CMV pneumonitis, increased numbers of both CD4+ T helper and CD8+ suppressor/cytotoxic pulmonary lymphocytes have been observed [ 6 ]. (biomedcentral.com)
  • Absolute number and percentages of CD3(+)CD4(+) and CD3(+)CD8(+) T lymphocytes, CD3(-)CD56(+) Natural Killer cells (NK cells) and NK cell cytotoxic activity was measured from venous blood. (isharonline.org)
  • We report here the distributions of lymphocyte populations bearing the following antigens: CD3 (T cells), CD19 (B cells), CD4 (T helper/inducer cells), CD8 (T suppressor/cytotoxic and some NK cells), and CD3-, CD16+, and/or CD56+ (NK cells). (ox.ac.uk)
  • Changes in lymphocyte subsets and cytokines during European porcine reproductive and respiratory syndrome: increased expression of IL-12 and IL-10 and proliferation of CD4(-)CD8(high). (surrey.ac.uk)
  • Cytokines are small protein molecules such as IL-6, IL-10, and TNF-α secreted by T lymphocytes which play a great role in intercellular communications and immunomodulation function [ 9 ]. (biomedcentral.com)
  • Furthermore, we assessed the susceptibility and permissiveness of various functionally distinct T and B cells, such as helper T (T H ) cell subsets and IgG- and IgA-positive memory B cells, in peripheral blood and tonsils. (asm.org)
  • T-lymphocyte and helper/suppressor cell immune status assist in evaluating cellular immunocompetency. (specialtylabs.com)
  • Greater numbers of helper T lymphocytes (Leu-3+) were found at the periphery than in the central portion of the same granuloma. (semanticscholar.org)
  • Counting the number of helper T-cells -- technically called CD4+ lymphocytes -- is perhaps the most important tool used to assess the overall health of the immune system in people with HIV. (thebody.com)
  • CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells (TFH cells). (wikipedia.org)
  • We also found lower percentage activated subsets T cells with phenotype CD3+HLA-DR before (x = 4.78) and after treatment (x = 4.03) compared with control (x = 7.27). (prohealth.com)
  • While T cells activated in hypoxia divided less than those activated in atmospheric oxygen, Glut1-Hi lymphocytes exhibited increased effector phenotype acquisition, augmented proliferation, and an inverted CD4/CD8 ratio in both oxygen conditions. (cnrs.fr)
  • As a result, lymphocyte immune phenotype analysis can be used as an important reference index for evaluating the body's immune status. (biomedcentral.com)
  • 1. CD4 counts were linearly related to total lymphocyte concentrations (Pearson correlation coefficient = 0.948), but no relationship was found between total lymphocyte or lymphocyte subset counts and APACHE II score, predicted mortality rate, or survival rate. (nih.gov)
  • The quantity of factor VIII concentrate given to boys with severe haemophilia A (mean U/year) showed a significant inverse correlation with total white cell counts, lymphocyte counts, platelet counts, and the ratio of monoclonal antibody defined T lymphocyte subsets, T4 and T8 (T4:T8). (bmj.com)
  • Treatment with high dose factor VIII concentrate (more than 25 000 U/year) was associated with low platelet counts, low lymphocyte counts, low T4:T8 ratios, and hypergammaglobulinaemia. (bmj.com)
  • The most commonly followed lymphocyte subsets include CD4 and CD8 cell counts and percentages. (thebody.com)
  • Relative counts of peripheral blood T and B lymphocyte subsets were detected by flow cytometry analysis. (biomedcentral.com)
  • CD4+ lymphocyte percentages and absolute counts may be used as aid in evaluation of immune competency. (thumbaylabs.com)
  • Following fingolimod discontinuation lymphocyte counts return to be within the normal range within 6-8 weeks and are above 80% of baseline values by 3 months. (blogspot.com)
  • Therefore low lymphocyte counts in fingolimod-treated MSers does not have the same biological implications as low lymphocyte counts in other situations. (blogspot.com)
  • Importantly, the effectiveness of fingolimod and adverse events is not related to the peripheral lymphocyte counts. (blogspot.com)
  • In contrast the European Medicine Agency (EMA) has recommended the need to check peripheral lymphocyte counts before initiating treatment and have recommended periodic testing at months 3 and then at least yearly after that. (blogspot.com)
  • Until we get more clarity on this we have recommended more frequent blood count monitoring in MSers with lymphocyte counts less than 200, i.e. 3 monthly. (blogspot.com)
  • The absolute counts of lymphocyte subsets are known to be influenced by a variety of biological factors, including hormones, the environment, and temperature. (testcatalog.org)
  • The studies on diurnal (circadian) variation in lymphocyte counts have demonstrated progressive increase in CD4 T-cell count throughout the day, while CD8 T cells and CD19+ B cells increase between 8:30 a.m. and noon with no change between noon and afternoon. (testcatalog.org)
  • Normal lymphocyte subsets values among children from Cameroon differ from reported values in Caucasian and some African populations. (nih.gov)
  • Nevertheless, B lymphocytes are one of the most prevalent leukocyte populations in the naive murine heart. (jci.org)
  • As a result, immunophenotyping using multi-color flow cytometry has become one of the most powerful techniques for profiling immune cell subsets within complex populations such as peripheral blood and tissue/tumor samples. (fcslaboratory.com)
  • This study used a lysed whole blood method for analysis of lymphocyte sub-populations in samples from children born and living in two districts: a highly-contaminated area (Michalovce) and one (Svidnik/Stropkov) with ≈≈ 2-fold lower environmental PCB levels. (elsevier.com)
  • There are various subsets of each of these individual populations with specific cell-surface markers and function. (testcatalog.org)
  • Observation of percentages changes of lymphocytes (CD19), T(CD3), subsets CD4, CD8, NK cells, activated lymphocytes with fenotype CD3+HLA-DR, CD4/CD8 rate in Lyme borreliosis was carried out before (I examination) and after antibioticotherapy (II examination). (prohealth.com)
  • In addition, the sum of the percentages of BB3+ and delta-TCS-1+ cells approximated the percentages of peripheral blood CD3+WT31- lymphocytes in seven normal donors tested. (rupress.org)
  • CD3, CD4 and CD8 percentages are reported as a percent of total lymphocytes. (aruplab.com)
  • In addition, the sum of the percentages of BB3 + and δ-TCS-1 + cells approximated the percentages of peripheral blood CD3 + WT31 - lymphocytes in seven normal donors tested. (elsevier.com)
  • Variations in T lymphocyte subsets and monocyte percentages between organizations were likened using the MannCWhitney check for 2 organizations and KruskalCWallis check for a lot more than 3 organizations, as all guidelines normally weren't distributed. (labsmart.net)
  • Wilcoxon signed rank check was utilized to review T lymphocyte monocyte and subsets percentages before and after kidney transplantation. (labsmart.net)
  • This study aimed to analyze the role of lymphocyte subsets in the immunopathogenesisof COVID-19 and severe influenza A, and examined the clinical significance of their alterations in the prognosis and recovery duration. (researchsquare.com)
  • The resulting dataset included median antibody binding capacities (ABCs) and percentage of positivity for all markers on all subsets and was developed into an interactive CD Maps web resource. (frontiersin.org)
  • Percentage of lymphocytes measured in area D1+D2 of the XN WDF and W1/W2 ratio from the WPC channel. (clinicaltrials.gov)
  • Changes of immune function relevant inflammatory factor of IL-6, TNF-α concentration, regulatory T cell (Tregs), T-lymphocyte subsets, CD4+T cell, CD8+T cell, Natural Killer cell (NK) percentage in vivo before and after treatment in two groups were detected and analysed. (alliedacademies.org)
  • A small percentage of human T lymphocytes, predominantly CD8 + T cells, express receptors for HLA class I molecules of natural killer type (NK-R) that are inhibitory for T-cell antigen receptor (TCR)-mediated functions. (elsevier.com)
  • Receiver operating quality (ROC) curve was useful for analyzing the perfect percentage of T cell subsets for identifying cutoff factors that yielded the best level of sensitivity and specificity to tell apart an bout of severe cellular rejection. (labsmart.net)
  • Using the resource, we examined differentially expressed proteins between granulocyte, monocyte, and dendritic cell subsets, and profiled dynamic expression of markers during thymocyte differentiation, T-cell maturation, and between functionally distinct B-cell subset clusters. (frontiersin.org)
  • Transmigration of lymphocyte subsets across these barriers and their activation/differentiation within the periphery and intrathecal compartments in health and autoinflammatory CNS disease are complex. (mdpi.com)
  • Mathematical models are warranted that qualitatively and quantitatively predict the distribution and differentiation stages of lymphocyte subsets in the blood and CSF. (mdpi.com)
  • We applied an immunoperoxidase technique performed on glass slides using the monoclonal antibodies, OKT3, OKT4, OKT8, OKIa, and Leu-7 to study T-cell subsets in bronchoalveolar lavage fluid and blood of 11 normal nonsmokers and 12 smokers. (nih.gov)
  • Two subsets of human T lymphocytes expressing gamma/delta antigen receptor are identifiable by monoclonal antibodies directed to two distinct molecular forms of the receptor. (rupress.org)
  • Distribution of T-lymphocyte subsets in different portions of sarcoid granulomas: immunohistologic analysis with monoclonal antibodies. (semanticscholar.org)
  • These results indicate that the pathogenesis of ulcerative colitis or Crohn's disease does not depend upon an alteration in the proportion of circulating T lymphocytes nor upon an imbalance of T lymphocyte subsets as defined by monoclonal antibodies. (ox.ac.uk)
  • Stable levels of CD8+ effector cells were observed together with a lack of association of CD8+ lymphocytes with graft function, such as GFR and serum creatinine. (harvest-re.com)
  • This reagent is for research purposes only: this kit is used to determine the content of T-Subset in human serum, plasma, tissue fluid and related fluid samples. (toyscalm.com)
  • However, the identification of immune cells such as macrophages and T lymphocytes in atherosclerotic plaques sparked the interest of researchers in understanding the precise roles of these cells in atherosclerosis. (springer.com)
  • Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa characterized by destruction of basal cells by Langerhans cells, macrophages, and T lymphocytes. (nih.gov)
  • Specifically, NKG2D is expressed on the surface of most NK cells, activated CD8 + αβ T cells, antigen-specific CD8 + memory T cells, a subset of γδ T cells and on activated macrophages ( 10 , 17 ). (aacrjournals.org)
  • Macrophages are directly involved in the fight against invading micro-organisms as well as being antigen-presenting cells which activate lymphocytes. (wikipedia.org)
  • Lymphocyte subsets are one important clinical test that can reveal the state of cellular immune and humoral immune. (chinesemedicinekidney.com)
  • At Flow Contact Site Laboratory, LLC we offer a variety of panels for immunophenotyping of lymphocyte subsets and other immune cell subsets for both preclinical and clinical studies. (fcslaboratory.com)
  • Further studies involving a larger patient population are needed to evaluate if different lymphocytes subset may be involved in different clinical phenotypes of IgM-related neuropathy. (unicatt.it)
  • However, although in vivo lymphocyte expansion has been correlated with good clinical outcome following DLI, the specificity and functional characteristics of EBV-specific T-cell responses remain poorly characterized. (frontiersin.org)
  • The aim of this study was to investigate the clinical usefulness of BAL cellular analysis with lymphocyte subsets for the differential diagnosis of DILDs. (bvsalud.org)
  • The clinical significance of circulating T-lymphocyte subsets and human being leukocyte antigen (HLA)-DR-positive monocytes in the peripheral blood vessels of kidney transplant recipients (KTRs) continues to be unclear. (labsmart.net)
  • In the clinical studies ~20% of study subjects had a least one lymphocyte count below this level. (blogspot.com)
  • Moroccan lymphocyte subsets reference ranges: age, gender, ethnicity, and socio-economic factors dependent differences. (medworm.com)
  • This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. (medworm.com)
  • It substantially duplicated a previous publication ("Lymphocyte Subset Reference Ranges in Healthy Saudi Arabian Children," by S. Shahabuddin, I. H. Al-Ayed, M. O. Gad El-Rab, and M. I. Qureshi, Pediatr. (asm.org)
  • Reference ranges for lymphocyte subsets in healthy adult male Omanis. (elsevier.com)
  • OBJECTIVE: To determine the reference ranges of lymphocyte subsets in serologically HIV-seronegative healthy male adults in Oman. (elsevier.com)
  • Lymphocyte subset reference ranges in adult Caucasians. (ox.ac.uk)
  • It appears possible to recommend reference ranges for lymphocyte population parameters applicable across national and laboratory boundaries. (ox.ac.uk)
  • Abnormalities in the proportions of immunoregulatory T-lymphocytes in bronchoalveolar lavage fluid have been reported in interstitial pulmonary diseases, yet the effect of cigarette smoking on T-cell subsets in bronchoalveolar lavage fluid from normal subjects has not been investigated. (nih.gov)
  • These abnormalities of lymphocyte subsets in bronchoalveolar lavage fluid correlated with the cumulative pack-years of smoking history but were not reflected in the peripheral blood. (nih.gov)
  • We determined the effects of gender, race, and age on the following lymphocyte subset markers: total CD4 + cells, CD4 + naïve cells, CD4 + memory cells, all CD8 + cells, CD8 + naïve cells, CD8 + memory cells, CD16 + natural killer cells, and CD19 + B cells. (asm.org)
  • This particular Treg subset co\expresses the activation marker HLA\DR and appears to impact effector cells functionally by release of TGF\ or via CTLA\4\mediated cell conversation with DC in lymph nodes. (harvest-re.com)
  • Thus, Glut1 surface levels identify human T lymphocytes with distinct effector functions in both hypoxic and atmospheric oxygen tensions. (cnrs.fr)
  • Lymphocytes strongly contribute to the pathology of multiple sclerosis (MS). Thereby, frequently activated or reactivated autoreactive lymphocytes differentiate into effector cells that enter the central nervous system (CNS) causing tissue inflammation and damage. (biomedcentral.com)
  • The immunomodulatory drug fingolimod (FTY720, Gilenya) directly targets S1P receptors and inhibits lymphocyte egress, whereas the exact effector function of interferon-β (IFN-β) in MS therapy remains elusive. (biomedcentral.com)
  • Flow cytometry analysis of lymphocyte subset markers was performed for a group of sexually active, human immunodeficiency virus (HIV)-negative adolescents over a 2-year period to establish normative data. (asm.org)
  • Lysis of autologous tumor cells by peripheral blood lymphocytes treated with interleukin 2 in patien. (biomedsearch.com)
  • Lymphocyte subsets, phytohaemagglutinin responsiveness of blood lymphocytes, and interleukin 2 production in sarcoidosis. (bmj.com)
  • Here, we present the results of the first phase of the CD Maps study, mapping the expression of CD1-CD100 ( n = 110) on 47 immune cell subsets from blood, thymus, and tonsil using an eight-color standardized EuroFlow approach and quantification of expression. (frontiersin.org)
  • Immunophenotyping is a technique to analyze the immune cell subsets by detecting proteins expressed on the surface or within the cell (intracellular) using antigen specific antibodies. (fcslaboratory.com)
  • Drawing a on the wealth of knowledge found in the literature, combinations of antigen-specific antibodies which are tagged with different fluorochromes can be put together to design antibody panels for the identification of specific immune cell subsets. (fcslaboratory.com)
  • Significant information can be gained by identifying these cellular subsets and monitoring the impact of a given treatment on the distribution of these immune cell subsets. (fcslaboratory.com)
  • Our findings suggested that abnormalities of peripheral lymphocyte subsets were related to the immune disorder of lupus itself, regardless of immunosuppressive treatment. (scielo.br)
  • Sphingosine-1-phosphate (S1P) is a key mediator of lymphocyte egress that binds to five S1P receptors (S1P1-5). (biomedcentral.com)
  • Furthermore, TCR-stimulated human T lymphocytes sorted on the basis of Glut1-Lo and Glut1-Hi profiles maintained distinct characteristics, irrespective of the oxygen tension. (cnrs.fr)
  • Since similar molecular patterns were consistently detected among all BB3 + as well as among all δ-TCS-1 + clones analyzed, we conclude that BB3 and δ-TCS-1 mAbs identify two distinct molecular forms of TCR-γ/δ expressed by two different peripheral blood T cell subsets. (elsevier.com)
  • LHTL could affect T lymphocyte subsets significantly as a result of the two simultaneous stimuli of physical activity and exposure to hypoxia. (bmj.com)
  • Notably, Glut1 upregulation in response to TCR stimulation was significantly higher in T lymphocytes activated under hypoxic as compared to atmospheric oxygen conditions. (cnrs.fr)
  • Moreover, Glut1-Hi T lymphocytes exhibited a significantly enhanced ability to produce IFN-gamma and this secretion potential was completely dependent on continued glycolysis. (cnrs.fr)
  • Compared with the less-contaminated region, Michalovce samples showed significantly higher expression of CD3 + T-lymphocytes, B-lymphocytes, and activated B-lymphocytes, whereas NK cells were less expressed. (elsevier.com)
  • Lymphocytes and their subsets, NK cells were signed and measured by flow cytometric immunophenotyping in Coulter EPIX XL with Becton-Dickinson antibodies twice: before and after treatment. (prohealth.com)
  • Some examples of our immunophenotyping of specific lymphocyte subsets in various species are shown below. (fcslaboratory.com)
  • Lymphocyte immunophenotyping is an important tool in the diagnosis of immunologic and hematologic disorders. (elsevier.com)
  • CONCLUSION: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adult males in Oman. (elsevier.com)
  • CD4 and CD8 T lymphocyte activation requires the generation of sufficient energy to support new biosynthetic demands. (cnrs.fr)
  • Increased numbers of central memory CD8 + T lymphocytes with increased pro-inflammatory activation are found in the blood of CRPS participants compared to healthy controls. (biomedcentral.com)
  • Initial models of highly stable T‐cell subsets were useful but underestimated diversity. (els.net)
  • Correlations between T-lymphocyte subset values and stages or cell types of disease were sought. (biomedsearch.com)
  • In a cohort of 352 healthy children, aged 0 to 6 years, the relative and absolute numbers of T-cell subsets, B cells, and NK lymphocytes were determined from peripheral blood collected in EDTA tubes. (nih.gov)
  • Current methods of polychromatic flow cytometry and PCR-based detection of k deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. (muni.cz)
  • In regards to lymphocytes in the peripheral blood, the machine has the capacity to distinguish activated from non-activated T-cell subsets using a very small volume of EDTA sample (88uL) (including remnant sample from a standard full blood count) with results available in 1.5 minutes. (clinicaltrials.gov)
  • Within the splenic and lymph node CD4+ T cell compartment, Ly-6C expression is restricted to Ly-6.2 strains of mice, and is present on a subset of naive cells. (jimmunol.org)
  • K. Yuki, S. G. Soriano and M. Shimaoka, "Sedative Drug Modulates T-Cell and Lymphocyte Function-Associated Antigen-1 Function," Anesthesia & Analgesia, Vol. 112, No. 4, 2011, pp. 830-838. (scirp.org)
  • Alterations in immunoregulatory T-cell subsets in cigarette smokers. (nih.gov)
  • However, information on the relative susceptibility of B-cell subsets is scarce. (asm.org)
  • Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) is a rare B-cell lymphoma considered to be of germinal center (GC) derivation. (ovid.com)
  • Thus, different patterns of T-cell subset changes were demonstrated between CB MNCs and APB MNCs in response to IL-12 and/or IL-15. (eurekamag.com)
  • This study aimed to examine the relationship between altered immune status and HCV-related liver disease, by determining the changes in peripheral blood lymphocyte and natural killer (NK) cell subsets in these subjects. (hku.hk)
  • To explore influences of lenalidomide on multiple myeloma, inflammatory factor, regulatory T cell and T-lymphocyte subsets. (alliedacademies.org)
  • The costimulatory effect is greater on the CD8+ than the CD4+ T cell subset. (rupress.org)
  • Therefore, human T cell costimulation can be achieved pharmacologically with thalidomide, and preferentially in the CD8+ T cell subset. (rupress.org)
  • Our instrumentation allows up to 10 parameters to be measured in a single tube and our analysis software utilizes complex gating strategies for narrowing specific lymphocyte subsets from the larger pool of cell types present in the sample. (fcslaboratory.com)
  • Lymphocyte function is related to the numerous complex superficial cell membrane proteins on the cell surface. (biomedcentral.com)
  • Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) is a life-threatening complication of T-lymphocyte deplete allogeneic hematopoietic stem cell transplantation (allo-HSCT). (frontiersin.org)
  • The use of magnetized cells holds promise for further applications in automated genotoxic and immunological cell assays of mononuclear leukocyte subsets. (aaccjnls.org)
  • Each of these lymphocyte subsets can be identified by a combination of one or more cell surface markers. (testcatalog.org)
  • T-lymphocytes (T-cells) are white blood cells which take part in cell-mediated immunity. (wikipedia.org)
  • Cantor H and Boyse EA (1975) Functional subclasses of T‐lymphocytes bearing different Ly antigens. (els.net)
  • The lymphocyte subpopulation distributions for all antigens were found to be similar at all sites. (ox.ac.uk)
  • As Glut1 is upregulated on tumor cells in response to hypoxia, we assessed whether surface Glut1 levels regulate the antigen responsiveness of human T lymphocytes in both hypoxic and atmospheric oxygen conditions. (cnrs.fr)
  • Sequentially CD4+ T lymphocytes increased gradually in number from initial skin lesions and were major composition cells in the tumor lesions. (koreascience.or.kr)
  • This is particularly relevant to fingolimod, which is a non-depleting agent and traps lymphocytes in the peripheral lymph nodes. (blogspot.com)
  • No statistical changes in subsets with receptor for IL-2 before and after treatment was shown. (prohealth.com)
  • T lymphocyte subsets in inflammatory bowel disease: peripheral blood. (ox.ac.uk)
  • T-lymphocyte subsets in acute illness. (nih.gov)
  • Cross-sectional study, comparing Acute Physiology and Chronic Health Evaluation II (APACHE II) scores and the calculated, disease-specific, predicted mortality rate with T-lymphocyte subsets. (nih.gov)
  • Study question: Do participants who have chronic or acute viral infections i.e. untreated HIV, HIV-HCV with treated HIV and untreated HCV, untreated HCV monoinfection or untreated HBV monoinfection, have a significant excess of activated lymphocytes as measured by D1+D2 on the XN WDF channel using the XN-20 analyser? (clinicaltrials.gov)
  • The study results showed a great advantage to using the Aquios CL for automated lymphocyte subset enumeration. (labroots.com)
  • This assay is CD4+ lymphocyte subset enumeration using Pan Leukocyte (CD45+) Gating strategy. (thumbaylabs.com)
  • Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. (jimmunol.org)
  • The expression patterns of the Ly-6C Ag were examined on splenic and thymic lymphocyte subsets of Ly-6.1 and Ly-6.2 strains of mice using the rat mAb 15.1. (jimmunol.org)
  • Some parameters that were out of favorable limits (elevated expression of CD56, CD158a in T lymphocytes, decreased levels of CD4 T lymphocytes, up-regulated expression of HLA DR in CD8+ T cells and NK cells, elevated number of NK cells and increased NK cytotoxicity, increased and decreased expression of CD158a and CD8 in NK cells) were considered in our study to be immune deviations (ID) potentially predictive for IVF failure [17]. (termedia.pl)
  • However, IFN-β is known to induce CD69 expression, which by direct interaction, downmodulates S1P1 surface expression preventing lymphocyte egress. (biomedcentral.com)
  • b Intracellular signalling and functional marker expression levels in the FlowSoM cluster representing central memory CD8 + T lymphocytes. (biomedcentral.com)
  • We can evaluate specific lymphocyte subsets in human, rodent (rat/mice), NHP and dog specimens. (fcslaboratory.com)
  • No significant differences were found between patient and control groups in the proportions of circulating T lymphocytes or their subsets. (ox.ac.uk)
  • T-lymphocyte subsets (T-Subset) were added to the monoclonal antibody-coated wells in turn, followed by The HRP-labeled T lymphocyte subset (T-Subset) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. (toyscalm.com)
  • Gender was the demographic characteristic most frequently associated with differences in lymphocyte subset measures. (asm.org)
  • No major differences were seen between groups for IgG levels and lymphocyte subsets. (iospress.com)
  • There were no differences in T lymphocyte numbers between the patient groups and the disease control subjects. (ox.ac.uk)
  • Age and sex associated differences are substantial for some lymphocyte subsets (CD3, CD4, NK cells), and proper accounting of these effects is essential in evaluating the individual patient, if further disease-related variation is to be accurately and consistently assessed. (ox.ac.uk)
  • Lymphocyte Pathology in Human Colorectal Cancer. (scirp.org)
  • Chernyshov V, Dons'koi B, Sudoma I, Goncharova Y. Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood. (termedia.pl)
  • Thus, dietary nucleotides appear to exert actions on immature human neonate lymphocytes enhancing the in vivo production of Ig which may have a role in the defense capacity of neonates. (iospress.com)
  • The microtiter plate was coated with purified human T-lymphocyte subset (T-Subset) antibody to make a solid-phase antibody. (toyscalm.com)
  • The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of human T-lymphocyte subsets (T-Subset) in the sample was calculated by a standard curve. (toyscalm.com)
  • Magnetically tagged subsets of human lymphocytes for assays with laboratory robotics. (aaccjnls.org)
  • We have attached antibody-coupled magnetic microspheres to the surface of human T-lymphocytes before performing various assays. (aaccjnls.org)
  • Lymphocyte subsets were quantitated using flow cytometry. (bmj.com)
  • Blood samples collected into tubes containing ethylene-diamine-tetra acetic acid were investigated for lymphocyte subsets using flow cytometer. (elsevier.com)
  • Analysis of the T Lymphocyte Subsets Using Flow Cytometry in Sudden Deafness. (kjorl.org)