Surface Plasmon Resonance
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
Magnetic Resonance Imaging
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Magnetic Resonance Spectroscopy
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Protein Array Analysis
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Protein Structure, Secondary
Nuclear Magnetic Resonance, Biomolecular
Recombinant Fusion Proteins
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Quartz Crystal Microbalance Techniques
Sequence Homology, Amino Acid
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Fluorescence Resonance Energy Transfer
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Amino Acid Motifs
Amino Acid Substitution
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Protein Structure, Quaternary
Protein Interaction Domains and Motifs
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Equipment Failure Analysis
Binding Sites, Antibody
Interleukin-5 Receptor alpha Subunit
The role of homophilic binding in anti-tumor antibody R24 recognition of molecular surfaces. Demonstration of an intermolecular beta-sheet interaction between vh domains. (1/3140)The murine antibody R24 and mouse-human Fv-IgG1(kappa) chimeric antibody chR24 are specific for the cell-surface tumor antigen disialoganglioside GD3. X-ray diffraction and surface plasmon resonance experiments have been employed to study the mechanism of "homophilic binding," in which molecules of R24 recognize and bind to other molecules of R24 though their heavy chain variable domains. R24 exhibits strong binding to liposomes containing disialoganglioside GD3; however, the kinetics are unusual in that saturation of binding is not observed. The binding of chR24 to GD3-bearing liposomes is significantly weaker, suggesting that cooperative interactions involving antibody constant regions contribute to R24 binding of membrane-bound GD3. The crystal structures of the Fabs from R24 and chR24 reveal the mechanism for homophilic binding and confirm that the homophilic and antigen-binding idiotopes are distinct. The homophilic binding idiotope is formed largely by an anti-parallel beta-sheet dimerization between the H2 complementarity determining region (CDR) loops of two Fabs, while the antigen-binding idiotope is a pocket formed by the three CDR loops on the heavy chain. The formation of homophilic dimers requires the presence of a canonical conformation for the H2 CDR in conjunction with participation of side chains. The relative positions of the homophilic and antigen-binding sites allows for a lattice of GD3-specific antibodies to be constructed, which is stabilized by the presence of the cell membrane. This model provides for the selective recognition by R24 of cells that overexpress GD3 on the cell surface. (+info)
A soluble form of the avian hepatitis B virus receptor. Biochemical characterization and functional analysis of the receptor ligand complex. (2/3140)Avian hepatitis B virus infection is initiated by the specific interaction of the extracellular preS part of the large viral envelope protein with carboxypeptidase D (gp180), the primary cellular receptor. To functionally and biochemically characterize this interaction, we purified a soluble form of duck carboxypeptidase D from a baculovirus expression system, confirmed its receptor function, and investigated the contribution of different preS sequence elements to receptor binding by surface plasmon resonance analysis. We found that preS binds duck carboxypeptidase D with a 1:1 stoichiometry, thereby inducing conformational changes but not oligomerization. The association constant of the complex was determined to be 2.2 x 10(7) M-1 at 37 degreesC, pH 7.4, with an association rate of 4.0 x 10(4) M-1 s-1 and a dissociation rate of 1.9 x 10(-3) s-1, substantiating high affinity interaction of avihepadnaviruses with their receptor carboxypeptidase D. The separately expressed receptor-binding domain, comprising about 50% of preS as defined by mutational analysis, exhibits similar constants. The domain consists of an essential element, probably responsible for the initial receptor contact and a part that contributes to complex stabilization in a conformation sensitive manner. Together with previous results from cell biological studies these data provide new insights into the initial step of hepadnaviral infection. (+info)
The L1 major capsid protein of human papillomavirus type 11 recombinant virus-like particles interacts with heparin and cell-surface glycosaminoglycans on human keratinocytes. (3/3140)The L1 major capsid protein of human papillomavirus (HPV) type 11, a 55-kDa polypeptide, forms particulate structures resembling native virus with an average particle diameter of 50-60 nm when expressed in the yeast Saccharomyces cerevisiae. We show in this report that these virus-like particles (VLPs) interact with heparin and with cell-surface glycosaminoglycans (GAGs) resembling heparin on keratinocytes and Chinese hamster ovary cells. The binding of VLPs to heparin is shown to exhibit an affinity comparable to that of other identified heparin-binding proteins. Immobilized heparin chromatography and surface plasmon resonance were used to show that this interaction can be specifically inhibited by free heparin and dextran sulfate and that the effectiveness of the inhibitor is related to its molecular weight and charge density. Sequence comparison of nine human L1 types revealed a conserved region of the carboxyl terminus containing clustered basic amino acids that bear resemblance to proposed heparin-binding motifs in unrelated proteins. Specific enzymatic cleavage of this region eliminated binding to both immobilized heparin and human keratinocyte (HaCaT) cells. Removal of heparan sulfate GAGs on keratinocytes by treatment with heparinase or heparitinase resulted in an 80-90% reduction of VLP binding, whereas treatment of cells with laminin, a substrate for alpha6 integrin receptors, provided minimal inhibition. Cells treated with chlorate or substituted beta-D-xylosides, resulting in undersulfation or secretion of GAG chains, also showed a reduced affinity for VLPs. Similarly, binding of VLPs to a Chinese hamster ovary cell mutant deficient in GAG synthesis was shown to be only 10% that observed for wild type cells. This report establishes for the first time that the carboxyl-terminal portion of HPV L1 interacts with heparin, and that this region appears to be crucial for interaction with the cell surface. (+info)
Cluster of differentiation antigen 4 (CD4) endocytosis and adaptor complex binding require activation of the CD4 endocytosis signal by serine phosphorylation. (4/3140)Cluster of differentiation antigen 4 (CD4), the T lymphocyte antigen receptor component and human immunodeficiency virus coreceptor, is down-modulated when cells are activated by antigen or phorbol esters. During down-modulation CD4 dissociates from p56(lck), undergoes endocytosis through clathrin-coated pits, and is then sorted in early endosomes to late endocytic organelles where it is degraded. Previous studies have suggested that phosphorylation and a dileucine sequence are required for down-modulation. Using transfected HeLa cells, in which CD4 endocytosis can be studied in the absence of p56(lck), we show that the dileucine sequence in the cytoplasmic domain is essential for clathrin-mediated CD4 endocytosis. However, this sequence is only functional as an endocytosis signal when neighboring serine residues are phosphorylated. Phosphoserine is required for rapid endocytosis because CD4 molecules in which the cytoplasmic domain serine residues are substituted with glutamic acid residues are not internalized efficiently. Using surface plasmon resonance, we show that CD4 peptides containing the dileucine sequence bind weakly to clathrin adaptor protein complexes 2 and 1. The affinity of this interaction is increased 350- to 700-fold when the peptides also contain phosphoserine residues. (+info)
Purified meningococcal transferrin-binding protein B interacts with a secondary, strain-specific, binding site in the N-terminal lobe of human transferrin. (5/3140)Neisseria meningitidis, grown in iron-limited conditions, produces two transferrin-binding proteins (TbpA and TbpB) that independently and specifically bind human serum transferrin (hTF) but not bovine serum transferrin (bTF). We have used surface plasmon resonance to characterize the interaction between individual TbpA and TbpB and a series of full-length human-bovine chimaeric transferrins (hbTFs) under conditions of variable saturation with iron. A comparative analysis of hTF and hbTF chimaera-binding data confirmed that the major features involved in Tbp binding are located in the C-terminal lobe of hTF and that isolated TbpA can recognize distinct sites present in, or conformationally influenced by, residues 598-679. Binding by TbpB was maintained at a significant but decreased level after replacement of the entire hTF C-terminal lobe by the equivalent bovine sequence. The extent of this binding difference was dependent on the meningococcal strain and on the presence of hTF residues 255-350. This indicated that TbpB from strain SD has a secondary, strain-specific, binding site located within this region, whereas TbpB from strain B16B6 does not share this recognition site. Binding of TbpA was influenced primarily by sequence substitutions in the hTF C-terminal lobe, and co-purified TbpA and TbpB (TbpA+B) was functionally distinct from either of its components. The limited divergence between hTF and bTF has been related to observed differences in binding by Tbps and has been used to delineate those regions of hTF that are important for such interactions. (+info)
Kinetics of the interaction of endotoxin with polymyxin B and its analogs: a surface plasmon resonance analysis. (6/3140)Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful side effects. Many peptide-based drugs mimicking the activity of PMB have been synthesized in an attempt to reduce toxicity while still retaining the anti-endotoxic activity. The study attempts to use the recent technique of surface plasmon resonance (SPR), in determining the kinetics of association and dissociation involved in the interaction of endotoxin with a few selected peptides that have structural features resembling PMB. The results, in conjunction with the thermodynamic data derived using isothermal titration calorimetry (ITC), stress the vital role played by amphiphilicity of the peptides and hydrophobic forces in this biologically important interaction. (+info)
Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptides. (7/3140)A fluorescein-labeled synthetic peptide, PTTTPITTTTK, was converted into O-glycosylated glycopeptides with various numbers of attached N-acetyl-D-galactosamines (GalNAcs) by in vitro glycosylation with UDP-GalNAc and a microsomal fraction of LS174T human colon carcinoma cells. Glycopeptides with 1, 3, 5, and 6 GalNAc residues (G1, G3, G5, and G6) were obtained, and their sizes were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Their sequences were determined by a peptide sequencer to be PTTTGalNAcPITTTTK for G1, PTGalNAcTTPITGalNAcTGalNAcTTK for G3, PTTGalNAcTGalNAcPITGalNAcTGalNAcTGalNAcTK for G5, and PTGalNAcTGalNAcTGalNAcPITGalNAcTGalNAcTGalNAcTK for G6. A calcium-type human macrophage lectin (HML) was prepared in a recombinant form, and its interaction with these glycopeptides was investigated by surface plasmon resonance (SPR) spectroscopy and fluorescence polarization. The affinity of recombinant HML (rHML) for immobilized glycopeptides increased, as revealed by SPR, in parallel with the number of GalNAc. The highest affinity was obtained when the G6-peptide was immobilized at high density. Fluorescence polarization equilibrium-binding assays also revealed that the affinity of rHML for soluble gly-copeptides increased, depending on the number of attached GalNAcs. Carbohydrate recognition domain (CRD) fragments of HML were prepared, and their affinity for these four glycopeptides was also determined, this affinity was apparently lower than that of rHML. Affinity constants of rHML for the G3- and G5-peptides were 11- and 38-fold higher, respectively, than for the G1-peptide, whereas those of CRD fragments were only 2- and 6-fold higher, respectively. A chemical cross-linking study revealed that rHML but not recombinant CRD forms trimers in an aqueous solution. Thus, preferential binding of densely glycosylated O-linked glycopeptides should be due to the trimer formation of rHML. (+info)
Effects of microcystins on phosphorylase-a binding to phosphatase-2A: kinetic analysis by surface plasmon resonance biosensor. (8/3140)Cyclic heptapeptide microcystins are a group of hepatoxicants which exert the cytotoxic effects by inhibiting the catalytic activities of phosphatase-2A (PP-2A) and phosphatase-1 (PP-1) and thus disrupt the normal signal transduction pathways. Microcystins interact with PP-2A and PP-1 by a two-step mechanism involving rapid binding and inactivation of protein phosphatase catalytic subunit, followed by a slower covalent interaction. It was proposed that inactivation of PP-2A/PP-1 catalytic activity by microcystins precedes covalent adduct formation. In this study, we used a biosensor based on surface plasmon resonance (SPR) to examine the effects of three microcystins, MCLR, MCRR and MCYR, on the binding between PP-2A and its substrate, phosphorylase-a (PL-a), during the first step of the interaction. The SPR biosensor provides real-time information on the association and dissociation kinetics of PL-a with immobilized PP-2A in the absence and presence of microcystins. It was found that the affinity of PL-a to microcystin-bound PP-2A was four times smaller compared to unbound PP-2A, due to 50% decreases in the association rates and two-fold increases in dissociation rates of PL-a binding to PP-2A. The results suggest that the rapid binding of microcystins to the PP-2A catalytic site leads to the formation of a noncovalent microcystin/PP-2A adduct. While the adduct formation fully inhibits the catalytic activity of PP-2A, it only results in partial inhibition of the substrate binding. The similar effects of the three microcystins on PP-2A suggest that the toxins bind to PP-2A at the same site and cause similar conformational changes. The present work also demonstrates the potential application of biosensor technology in environmental toxicological research. (+info)
Narrow groove plasmonic nano-gratings for surface plasmon resonance sensing
1. J. Homola, S. S. Yee, and G. Gauglitz, Surface plasmon resonance sensors: Review, Sens. Actuators B Chem. 54(1-2), 3-15 (1999). [CrossRef] 2. R. C. Jorgenson and S. S. Yee, A fiber optic chemical sensor based on surface plasmon resonance, Sens. Actuators B Chem. 12(3), 213-220 (1993). [CrossRef] 3. J. Homola, Surface Plasmon Resonance Based Sensors (Springer, Berlin, 2006).. 4. J. Čtyroký, J. Homola, and M. Skalský, Tuning of spectral operation range of a waveguide surface plasmon resonance sensor, Electron. Lett. 33(14), 1246-1248 (1997). [CrossRef] 5. R. Slavík, J. Homola, J. Čtyroký, and E. Brynda, Novel spectral fiber optic sensor based on surface plasmon resonance, Sens. Actuators B Chem. 74(1-3), 106-111 (2001). [CrossRef] 6. P. Schuck, Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromolecules, Annu. Rev. Biophys. Biomol. Struct. 26(1), 541-566 (1997). [CrossRef] [PubMed] 7. M. Malmqvist, Surface ...
Using U-Shaped Localized Surface Plasmon Resonance Sensors to Compensate for Nonspecific Interactions - IEEE Journals & Magazine
We report a simple label-free localized surface plasmon resonance sensor that uses the multiple resonances of a U-shaped gold nanostructure to differentiat
Sensors | Free Full-Text | Rational Design of Peptide-Functionalized Surface Plasmon Resonance Sensor for Specific Detection of...
In this study, a rationally-designed 2,4,6-trinitrotoluene (TNT) binding peptide derived from an amino acid sequence of the complementarity-determining region (CDR) of an anti-TNT monoclonal antibody was used for TNT detection based on a maleimide-functionalized surface plasmon resonance (SPR) sensor. By antigen-docking simulation and screening, the TNT binding candidate peptides were obtained as TNTHCDR1 derived from the heavy chain of CDR1, TNTHCDR2 derived from CDR2, and TNTHCDR3 from CDR3 of an anti-TNT antibody. The binding events between candidate peptides and TNT were evaluated using the SPR sensor by direct determination based on the 3-aminopropyltriethoxysilane (APTES) surface. The TNT binding peptide was directly immobilized on the maleimide-functionalized sensor chip surface from N-γ-maleimidobutyryl-oxysuccinimide ester (GMBS). The results demonstrated that peptide TNTHCDR3 was identified and selected as a TNT binding peptide among the other two candidate peptides. Five kinds of TNT
OSA | Rational design of high performance surface plasmon resonance sensors based on two-dimensional metallic hole arrays
We have rationally designed two-dimensional Au and Ag hole arrays for high performing surface plasmon resonance (SPR) sensing. The figure-of-merit (FOM), which is defined as sensitivity/linewidth, is found to be highly geometry-dependent. For sensitivity, we find it is equal to the period of array when exciting low order surface plasmon modes at low incident angle. Therefore, increasing period improves sensitivity. On the other hand, narrow linewidth can be obtained from small hole size so that the radiative decay loss is minimized. By using a pair of orthogonally oriented polarizer and analyzer, the signal-to-noise ratio (SNR) can be greatly enhanced due to the elimination of the nonresonant reflection background. As a proof of our strategy, we have obtained FOM larger than 100/RIU and SNR higher than 110 from Au arrays. Our results show the importance of understanding the basic properties of surface plasmon polaritons in order to systematically optimize the performance of the plasmonic system ...
AID 642885 - Binding affinity to chicken riboflavin binding protein by surface plasmon resonance assay - PubChem
BioAssay record AID 642885 submitted by ChEMBL: Binding affinity to chicken riboflavin binding protein by surface plasmon resonance assay.
Complement C3b interactions studied with surface plasmon resonance technique
The surface plasmon resonance (SPR) phenomenon is utilized in a number of new real time biosensors. In this study, we have used this technique to study interactions between the central complement component C3b and its multiple ligands by using the Biacore equipment. The SPR technique is particularly suitable for analysis of the alternative complement pathway (AP) because the inherent nature of the latter is to amplify deposition of C3b on various surfaces. C3b was coupled onto the sensor surface and the coupling efficiency was compared under various conditions on both polystyrene and carboxymethylated dextran surfaces. After enzymatic C3b coupling or standard amine C3b coupling, we analyzed and compared the binding of four C3b ligands to the surface: factor B, factor H, C5 and the soluble complement receptor 1 (sCR1, CD35). Binding of each ligand to C3b was detected when C3b had been coupled either enzymatically or using the amine coupling, but the half-lives of the interactions were found to ...
HIGH SENSITIVITY PLASMONIC STRUCTURES FOR USE IN SURFACE PLASMON RESONANCE SENSORS AND METHOD OF FABRICATION THEREOF -...
0035] Generally stated, the non-limitative illustrative embodiment described hereinafter relates to a high sensitivity plasmonic structure for use in a surface plasmon resonance (SPR) sensor, and a method of fabrication thereof. The plasmonic structure comprises an array of microholes defining triangles of 700 nm, 950 nm and 1.8 μm edge lengths, which transition to propagating SPR with microhole arrays of decreasing size. Such microhole arrays exhibit a short range SPR mode (as measured in the Kretschmann configuration SPR). Triangle arrays of different sizes and aspect ratio generally exhibit two absorption bands and a transmission maximum in the SPR spectrum. The maximum in transmission at approximately λ=600 nm exhibits the best analytical characteristics for triangle arrays. This maximum shifts significantly with increasing refractive index (RI) for the triangles of 950 nm and 1.8 μm edge lengths, with a sensitivity of 1993 and 1038 nm/RI respectively. This high sensitivity is comparable ...
Surface Plasmon Resonance Technique for Studies of Protein Adsorption on Biomaterials
1996 (English)In: 3: rd Meeting and Seminar on : Ceramics,Cells and Tissues, (Ed. Ravaglioli,A. and Krajewski,A.). Gruppo editoriale faenza editrice s.p.a., 1996, 171-178 p.Conference paper, Published paper (Other scientific) ...
Characterization of a new maleimido functionalization of gold for surface plasmon resonance spectroscopy
Publikations-Datenbank der Fraunhofer Wissenschaftler und Institute: Aufsätze, Studien, Forschungsberichte, Konferenzbeiträge, Tagungsbände, Patente und Gebrauchsmuster
Enhancement of surface plasmon resonance sensing for DNA hybridization using colloidal Au attached probe DNA<...
TY - JOUR. T1 - Enhancement of surface plasmon resonance sensing for DNA hybridization using colloidal Au attached probe DNA. AU - Yamaguchi, Akira. AU - Juodkazis, Saulius. AU - Matsuo, Shigeki. AU - Misawa, Hiroaki. PY - 2002/2/5. Y1 - 2002/2/5. N2 - In this study, we demonstrate that the Au particle modified probe DNA monolayer can enhance the surface plasmon resonance (SPR) signal for measuring hybridization of unlabeled DNA molecules. The Au particles adsorbed on single stranded (ss)- and double stranded (ds)-DNA monolayers have different optical interaction with surface of Au thin film, and this difference induces the enhancement of the SPR signal.. AB - In this study, we demonstrate that the Au particle modified probe DNA monolayer can enhance the surface plasmon resonance (SPR) signal for measuring hybridization of unlabeled DNA molecules. The Au particles adsorbed on single stranded (ss)- and double stranded (ds)-DNA monolayers have different optical interaction with surface of Au thin ...
Ultraviolet Surface Plasmon-Coupled Emission Using Thin Aluminum Films
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Differential roles of ionic, aliphatic, and aromatic residues in membrane - Protein interactions: A surface plasmon resonance...
TY - JOUR. T1 - Differential roles of ionic, aliphatic, and aromatic residues in membrane - Protein interactions. T2 - A surface plasmon resonance study on phospholipases A2. AU - Stahelin, R. V.. AU - Cho, W.. PY - 2001/4/17. Y1 - 2001/4/17. N2 - The roles of cationic, aliphatic, and aromatic residues in the membrane association and dissociation of five phospholipases A2 (PLA2), including Asp-49 PLA2 from the venom of Agkistodon piscivorus piscivorus, acidic PLA2 from the venom of Naja naja atra, human group IIa and V PLA2s, and the C2 domain of cytosolic PLA2, were determined by surface plasmon resonance analysis. Cationic interfacial binding residues of A. p. piscivorus PLA2 (Lys-10) and human group IIa PLA2 (Arg-7, Lys-10, and Lys-16), which mediate electrostatic interactions with anionic membranes, primarily accelerate the membrane association. In contrast, an aliphatic side chain of the C2 domain of cytosolic PLA2 (Val-97), which penetrates into the hydrophobic core of the membrane and ...
Monolayer formation of cytochrome b(562) on gold surfaces and its reconstitution reaction, studied by surface plasmon resonance...
Autor: Kobayashi, K. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2002-08; Titel: Monolayer formation of cytochrome b(562) on gold surfaces and its reconstitution reaction, studied by surface plasmon resonance spectroscopy
Sequential analysis of multiple analytes using a surface plasmon resonance (SPR) biosensor<...
TY - JOUR. T1 - Sequential analysis of multiple analytes using a surface plasmon resonance (SPR) biosensor. AU - Chung, J. W.. AU - Bernhardt, R.. AU - Pyun, J. C.. PY - 2006/4/20. Y1 - 2006/4/20. N2 - A sequential analysis method for the analysis of two analytes was developed using a surface plasmon resonance (SPR) biosensor. A sample with both analytes was introduced into the single sensing region and then each analyte was analyzed sequentially. Two detection models were devised for the samples with the following composition: (1) one target analyte resulting in a sensor response without any label and the other analyte with only additional label, (2) both target analytes requiring additional labels for detection. A standard curve for each model was prepared and applied for sequential analysis of anti-bovine serum albumin (anti-BSA) antibodies and horseradish peroxidase (HRP). The errors of the sequential analysis of Models 1 and 2 were found to be less than 6%, and this method was therefore ...
Protein sequence and surface plasmon resonance analysis | Open-i
Protein sequence and surface plasmon resonance analysis of the anti-IGFBP7 sdAb 4.43. (A) Protein sequence of anti-IGFBP7 sdAb 4.43; CDR1, CDR2 and CDR3 are und
Surface plasmon resonance-based biosensors: From the development of different SPR structures to novel surface functionalization...
en] Surface plasmon resonance (SPR)-based biosensors are very powerful tools for the study of biomolecular interactions, chemical detection and immunoassays. This paper reviews the performance of various SPR structures and detection schemes focusing on propagating surface plasmons generated in planar structures. Some aspects of their surface functionalization, the key element which imparts biofunctionality to these structures and hence transforming them into biosensors, will also be discussed accordingly. The ultimate performance of SPR-based biosensors will thus be determined by both their inherent optical performance and suitable surface functionalization. (C) 2011 Elsevier Ltd. All rights reserved ...
Analysis of the fine specificity of Tn-binding proteins using synthetic glycopeptide epitopes and a biosensor based on surface...
Using synthetic Tn (GalNAc-O-Ser/Thr) glycopeptide models and a biosensor based on surface plasmon resonance spectroscopy we have determined that isolectin B4 from Vicia villosa (VVLB4) binds to one Tn determinant whereas the anti-Tn monoclonal antibodies 83D4 and MLS128 require at least two Tn residues for recognition. When an unglycosylated amino acid is introduced between the Tn residues, both antibodies do not bind. MLS128 affinity was higher on a glycopeptide with three consecutive Tn residues. These results indicate that Tn residues organized in clusters are essential for the binding of these antibodies and indicate a different Tn recognition pattern for VVLB4.
New Publication - OpenSPR Phospholipid Binding Data Advances Cardiac Health Research
Dr. Zeczycki from East Carolina University, uses OpenSPRs surface plasmon resonance technology to get the key binding data needed for their recent publication on protein-lipid interaction.
Surface plasmon resonance phase imaging measurements of patterned monolayers and DNA adsorption onto microarrays<...
TY - JOUR. T1 - Surface plasmon resonance phase imaging measurements of patterned monolayers and DNA adsorption onto microarrays. AU - Halpern, Aaron R.. AU - Chen, Yulin. AU - Corn, Robert M.. AU - Kim, Donghyun. PY - 2011/4/1. Y1 - 2011/4/1. N2 - The optical technique of surface plasmon resonance phase imaging (SPR-PI) is implemented in a linear microarray format for real-time measurements of surface bioaffinity adsorption processes. SPR-PI measures the phase shift of p-polarized light incident at the SPR angle reflected from a gold thin film in an ATR Kretschmann geometry by creating an interference fringe image on the interface with a polarizer-quartz wedge depolarizer combination. The position of the fringe pattern in this image changes upon the adsorption of biomolecules to the gold thin film. By using a linear array of 500 μm biosensor element lines that are perpendicular to the interference fringe image, multiple bioaffinity adsorption measurements can be performed in real time. Two ...
Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on surface plasmon resonance. - PubMed -...
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Download Advances in Food and Nutrition Research, Vol. 54 by Steve Taylor PDF - Give It All I Book Archive
Bioelectron. 19, 1497-1504. Oh, B. , Chun, B. , Bae, Y. , Lee, W. , and Choi, J. W. (2005). The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosens. Bioelectron. 20, 1847-1850. , and Matsunaga, T. (1999). Electrochemical detection of allergen in small-volume whole blood using an array microelectrode: A simple method for detection of allergic reaction. Biotechnol. Bioeng. 65, 480-484. Okrend, A. J. , Rose, B. , and Lattuda, C. P. (1992). Isolation of Escherichia coli O157:H7 using O157 specific antibody coated beads. Lee, W. , and Choi, J. W. (2005). The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosens. Bioelectron. 20, 1847-1850. , and Matsunaga, T. (1999). Electrochemical detection of allergen in small-volume whole blood using an array microelectrode: A simple method for detection of allergic reaction. Biotechnol. Bioeng. 65, 480-484. Okrend, A. J. , Rose, B. , and Lattuda, C. P. (1992). Isolation ...
Micro integrated planar optical waveguide type SPR sensor - Patent # 7483140 - PatentGenius
An integrated optical waveguide type surface plasmon resonance (SPR) sensor having an optical waveguide with a corresponding SPR sensing area, photodetectors, and wavelength tunable laser or any kind of external tunable laser source/coupler formed on a substrate. In an embodiment, the laser is a wavelength tunable laser and optionally, the integrated device may include a power source on the substrate for providing a electric power to the wavelength tunable laser and the photodetectors, or a circuit for signal processing, or a microfluidic structure for routing a target sample to the SPR sensor area. The microfluidic structure optionally includes a mixer or a reaction chamber for mixing and allowing a physical or chemical reaction to occur, respectively. In an embodiment, plural planar integrated optical waveguide type SPR sensors may be fabricated on a substrate to form an array of SPR sensors.
Surface Plasmon Resonance
Surface Plasmon Resonance (SPR) is created by surface plasmons, coherent electron oscillations existing between any two materials where the real part of the dielectric function changes sign across the interface. SPR technology is based on the electromagnetic field component of incident light penetrating into a surface and it can be used to detect molecular adsorption on surfaces.. Plasmon excitation typically exists in two configurations: the Kretschmann-Raether configuration, where a thin metal film is sandwiched between a dielectric and air, and the incident wave is from the dielectric side; and the Otto configuration, where an air gap exists between the dielectric and the metal. Using COMSOL Multiphysics, the effect of the SPR on the electromagnetic field can be defined, which has allowed for the measurement of surface contaminants and nano-scale photonic devices.. ...
ENHANCING SURFACE PLASMON RESONANCE IMAGING SIGNAL - Patent application
0082] SPRi detection of biomolecular-binding interactions was performed using the SPRi Lab+ apparatus equipped with an 800 nm LED source, CCD camera and a flow cell (GenOptics, France), placed in Memmert Peltier-cooled incubator (Rose Scientific, Canada) for temperature control (for detailed system specifications, see L. Malic, B. Cui, M. Tabrizian, T. Veres, Nanoimprinted plastic substrates for enhanced surface plasmon resonance imaging detection, Opt. Express, 17, 20386-92 (2009)). The entire biochip surface was imaged during the angular scan, while for each experiment three ˜500 μm diameter spots were selected for the monitoring of the binding interactions with both the probe and the control. For each spot, the reflected intensity was displayed as a function of angle in the plasmon curve diagram. The slope of the plasmon curves was automatically computed to facilitate the selection of the working angle for kinetic analysis, which corresponded to the point of the plasmon curve at which the ...
화학공학소재연구정보센터(CHERIC) | 연구정보 | 문헌DB | 학술지 검색
The simultaneous detection of multiple analytes is an important consideration for the advancement of biosensor technology. Currently, few sensor systems possess the capability to accurately and precisely detect multiple antigens. This work presents a simple approach for the functionalization of sensor surfaces suitable for multichannel detection. This approach utilizes self-assembled monolayer (SAM) chemistry to create a nonfouling, functional sensor platform based on biotinylated single-stranded DNA immobilized via a streptavidin bridge to a mixed SAM of biotinylated alkanethiol and oligo(ethylene glycol). Nonspecific binding is minimized with the nonfouling background of the sensor surface. A usable protein chip is generated by applying protein-DNA conjugates which are directed to specific sites on the sensor chip surface by utilizing the specificity of DNA hybridization. The described platform is demonstrated in a custom-built surface plasmon resonance biosensor. The detection capabilities of ...
Shifting of surface plasmon resonance due to electromagnetic coupling between graphene and Au nanoparticles | [email protected]
Shifting of the surface plasmon resonance wavelength induced by the variation of the thickness of insulating spacer between single layer graphene and Au nanoparticles is studied. The system demonstrates a blue-shift of 29 nm as the thickness of the spacer layer increases from 0 to 15 nm. This is due to the electromagnetic coupling between the localized surface plasmons excited in the nanoparticles and the graphene film. The strength of the coupling decays exponentially with a decay length of d/R = 0.36, where d is the spacer layer thickness and R is the diameter of the Au nanoparticles. The result agrees qualitatively well with the plasmon ruler equation. Interestingly, a further increment of the spacer layer thickness induces a red-shift of 17 nm in the resonance wavelength and the shift saturates when the thickness of the spacer layer increases above 20 nm. © 2012 Optical Society of America ...
Fluorescence correlation spectroscopy in surface plasmon coupled emission microscope<...
TY - JOUR. T1 - Fluorescence correlation spectroscopy in surface plasmon coupled emission microscope. AU - Borejdo, J.. AU - Calander, N.. AU - Gryczynski, Z.. AU - Gryczynski, I.. PY - 2006. Y1 - 2006. N2 - Study of dynamics of single molecules by Fluorescence Correlation Spectroscopy (PCS) requires that the rate of photon detection per molecule be high, that the background be low, and that there be a large change in fluorescent signal associated with change in a position of a molecule. PCS applied to microscopic Surface Plasmon Coupled Emission (SPCE) suggests a powerful method to meet those requirements. In this method, the observational volume is made shallow by placing a sample on a thin metal film and illuminating it with the laser beam at Surface Plasmon Resonance (SPR) angle through high numerical aperture objective. The illuminating light excites surface plasmons in the metal film that produce an evanescent wave on the aqueous side of the interface. The thickness of the detection volume ...
Repositorio da Producao Cientifica e Intelectual da Unicamp: Development of an electroactive layer-by-layer assembly based on...
The development of a novel electrochemically stable host-guest supramolecular complex at a host surface is described. It was constructed by combining a self-assembled monolayer (SAM) of mono-(6-deoxy-6-mercapto)-beta-cyclodextrin (beta CDSH), iron (III) tetra-(N-methyl-4-pyridyl)-porphyrin (FeTMPyP) as the guest-link-molecule and beta-cyclodextrin-functionalized gold nanoparticles (beta CDAuNP). The building process of the layer-by-layer assembly was monitored by surface plasmon resonance spectroscopy (SPR). The binding processes between the host-functionalized gold surface and the linker molecule (FeTMPyP) were verified to be monovalent, and for host-functionalized gold nanoparticles with FeTMPyP, the interaction was determined to be bivalent. Finally, the electrochemical properties of the electroactive supramolecular multivalent film were determined. (C) 2009 Elsevier B.V. All rights reserved ...
Optimized sandwiched surface plasmon resonance enhanced biosensor for multiplex biomarker detection | DR-NTU
In this study, we performed finite element method (FEM) simulations to optimize the configuration of gold nanorods (GNR) enhanced surface plasmon resonance (SPR) sensor and discovered its application for multiplex antigens detection. Our work analyzed the near-field coupling between the sensing film and GNR. By systematically study the effect of gold film thickness, GNR-to-film distance and GNR dimensions on SPR, it was found that for GNR width smaller than 40nm, length change in GNR brought about significant SPR wavelength shift on the sensor, while the sensor is insensitive for GNR-to-film distance. As an application, we adopted GNRs of width 20 nm and aspect ratios from 2 to 4 and demonstrated the concept of conjugating gold film and GNRs with anti-Immunoglobulin G (anti-IgG) antibodies for multiplex detection of various IgG proteins with more than 100nm separation on their SPR wavelengths ...
Signal Amplification of Surface Plasmon Resonance based on Gold Nanoparticle-Antibody Conjugate and its Application to Protein...
Nanoparticles have offered many diverse capabilities in bioanalysis and biotechnological applications, due to the intrinsic properties of nanoparticle that is distinguished from bulk materials. The integration of nanoparticle, which exhibit unique electronic, photonic, and catalytic properties with biomaterial, which display unique recognition, catalytic, and inhibition property yields novel hybrid nanobiomaterial with synergic properties and functions. In this study, gold (Au) nanoparticle-antibody conjugate was applied for the signal enhancement of surface plasmon resonance (SPR). When small molecule is a target for immobilization or detection, the changes in the angle-dependent attenuated total reflectance (ATR) are often small so the use of SPR method is limited for generic application. The specific interaction between target molecules and Au nanoparticleantibody conjugate induce the change of the surface properties such as mass and roughness, which can be represented as the change of plasmon
Talk:Course Timetable - CellBiology
Pope ME, Soste MV, Eyford BA, Anderson NL, Pearson TW. Anti-peptide antibody screening: selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique. J Immunol Methods. 2009 Feb 28;341(1-2):86-96. Epub 2008 Nov 28. PMID: ...
Difference between revisions of Talk:Course Timetable - CellBiology
Pope ME, Soste MV, Eyford BA, Anderson NL, Pearson TW. Anti-peptide antibody screening: selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique. J Immunol Methods. 2009 Feb 28;341(1-2):86-96. Epub 2008 Nov 28. PMID: ...
Enzyme detection by surface plasmon resonance using specially engineered spacers and plasmonic labelling | (2011) | François |...
Surface Plasmon Resonance (SPR) is a powerful label free optical biosensing technology that relies on the measurement of the refractive index or change of mass in close vicinity of the sensor surface. Therefore, there is an experimental limitation in the molecular weight of the molecule that can be detected and consequently small molecules are intrinsically more difficult to detect using SPR. One approach to overcoming this limitation is to first adsorb smaller molecules onto the sensor surface, and to follow this by using their higher molecular weight antibodies counterparts which ensure the specificity (and are easier to detect via SPR due to their higher weight). Although this has been demonstrated with some success, it is not applicable in every case and some biomolecules such as enzyme are still difficult to detect due to their specific reactivity (enzymatic reaction). In this paper, we present a powerful new method that utilises specifically engineered spacers attached on one end to the ...
Localization of the putative sialic acid-binding site on the immunoglobulin superfamily cell-surface molecule CD22. - Oxford...
B-lymphocyte antigen CD22 is a member of the recently described sialoadhesin family of immunoglobulin-like cell-surface glycoproteins that bind glycoconjugates terminating in sialic acid. One prominent ligand for CD22 is the highly glycosylated leukocyte surface protein CD45. Using surface plasmon resonance spectroscopy, we characterized the interaction of recombinant mouse CD22 with native CD45 purified from rat thymus (CD45-thy). By in situ desialylation and resialylation of immobilized CD45-thy, we show that mouse CD22 binds to the sialoglycoconjugate NeuGc alpha 2-6Gal beta 1-4GlcNAc carried on CD45-thy N-glycans. Previous studies have shown that the sialic acid-binding site lies within the two membrane-distal domains of CD22 (domains 1 and 2), which are V-set and C2-set immunoglobulin superfamily domains, respectively. To further localize the binding site, we have made 42 single amino acid substitutions throughout both domains. All 12 mutations that abrogated binding to CD45-thy without disrupting
Zum Mechanismus der Signalübertragung durch G-Protein gekoppelte Rezeptoren
The human organism employs G protein coupled receptors (GPCRs), cell surface receptors in the plasma membrane, to transmit extracellular signals such as hormones, neurotransmitters, gustatory and olfactory signals into the interior of the cell. Specific binding of these extracellular ligands induces a conformational change in GPCRs, which allows the interaction with heterotrimeric G proteins and the catalysis of GDP/GTP nucleotide exchange in the G protein. The G protein then transmits the signal by protein-protein interaction to intracellular effector proteins. The publications summarized here on the rhodopsin/transducin system of photoreceptor cells, a model system for GPCRs/G proteins, cover three topics: 1. Methodology developments for biophysical monitoring of the interaction between rhodopsin and transducin by light scattering and evanescent field techniques, in particular surface plasmon resonance spectroscopy. 2. Formation of the active conformation of rhodopsin. The temporal sequence of ...
Original Research and Commentary on p28 CDG Therapeutics Lead Agent and Analogs. Binding of Amphipathic Cell Penetrating Peptide p28 to Wild Type and Mutated p53 as studied by Raman, Atomic Force and Surface Plasmon Resonance spectroscopies.. Signorelli S, Santini S, Yamada T, Bizzarri AR, Beattie CW, Cannistraro S.. Biochim Biophys Acta. 2017 Apr;1861(4):910-921.. Phase 1 trial of p28 (NSC745104), a non-HDM2-mediated peptide inhibitor of p53 ubiquitination in pediatric patients with recurrent or progressive central nervous system tumors: A Pediatric Brain Tumor Consortium Study.. Lulla RR, Goldman S, Yamada T, Beattie CW, Bressler L, Pacini M, Pollack IF, Fisher PG, Packer RJ, Dunkel IJ, Dhall G, Wu S, Onar A, Boyett JM, Fouladi M.. Neuro Oncol., 2016 Sep;18(9):1319-25. p28-mediated Activation of p53 in G2/M Phase of the Cell Cycle Enhances the Efficacy of DNA Damaging and Antimitotic Chemotherapy.. Yamada T, Das Gupta TK, Beattie CW.. Cancer Res. 2016 Feb 26; 76(8): 2354-2365. Chirality ...
Blood plasma coagulation studied by surface plasmon resonance | (1999) | Vikinge | Publications | Spie
A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real-time as a function of thromboplastin and heparin concentrations. The physical reason for the SPR signal observed is discussed and 3 different models are proposed. The response curves were analyzed by multivariable curve fitting followed by feature extraction. Interesting parameters of the sigmoid curves were lag time, slope and maximum response. When thromboplastin concentrations were increased, the lag-time decreased and the slope of the curve increased. A prolonged clotting time was followed mostly by increased maximum response, with exception for samples with no or very little thromboplastin added. High heparin concentrations changed the clotting kinetics. As seen from the lag-time vs. slope relation. Atomic force microscopy pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network structures as a function of thromboplastin concentration, and fiber ...
JSSS - Monitoring human serum albumin cell cultures using surface plasmon resonance (SPR) spectroscopy
Abstract. Continuously monitoring cell cultures is essential for both controlling critical parameters and improving understanding of key processes. An ideal technique in this context is surface plasmon resonance (SPR) spectroscopy, which essentially exploits changes in the angle of incident light that occur when molecules bind to a surface. It provides the ability to monitor real-time changes in small concentrations of various molecules, with no need for additional labels or sample preparation. Here we present an SPR-based immunoassay for monitoring concentrations of human serum albumin (HSA), and compare its sensitivity when used in conjunction with a Biacore platform and the cheaper, smaller liSPR system. In conjunction with either system, the immunoassay can detect HSA (a hepatocyte viability marker) at concentrations typically present in three-dimensional hepatocyte cultures mimicking the liver used to evaluate effects of drug candidates before exposure to humans or animals. Furthermore, in ...
Novel CD8+ T cell antagonists based on beta 2-microglobulin. - Radcliffe Department of Medicine
The CD8 coreceptor of cytotoxic T lymphocytes binds to a conserved region of major histocompatibility complex class I molecules during recognition of peptide-major histocompatibility complex (MHC) class I antigens on the surface of target cells. This event is central to the activation of cytotoxic T lymphocyte (CTL) effector functions. The contribution of the MHC complex class I light chain, beta(2)-microglobulin, to CD8alphaalpha binding is relatively small and is mediated mainly through the lysine residue at position 58. Despite this, using molecular modeling, we predict that its mutation should have a dramatic effect on CD8alphaalpha binding. The predictions are confirmed using surface plasmon resonance binding studies and human CTL activation assays. Surprisingly, the charge-reversing mutation, Lys(58) --| Glu, enhances beta(2)m-MHC class I heavy chain interactions. This mutation also significantly reduces CD8alphaalpha binding and is a potent antagonist of CTL activation. These results suggest a
Epitope Specificity Determines Pathogenicity and Detectability of Anti-Platelet-Derived Growth Factor Receptor α Autoantibodies...
Objective To identify the epitopes recognized by autoantibodies targeting platelet-derived growth factor receptor α (PDGFRα) in systemic sclerosis (SSc) and develop novel assays for detection of serum anti-PDGFRα autoantibodies. Methods Epstein-Barr virus-immortalized B cells from 1 patient with SSc (designated PAM) were screened for expression of IgG binding to PDGFRα and induction of reactive oxygen species in fibroblasts. The variable regions of anti-PDGFRα IgG were cloned into an IgG expression vector to generate distinct recombinant human monoclonal autoantibodies (mAb), which were characterized by binding and functional assays. The epitopes of anti-PDGFRα recombinant human mAb were defined by molecular docking, surface plasmon resonance binding assays, screening of a conformational peptide library spanning the PDGFRα extracellular domains, and expression analyses of alanine-scanned PDGFRα mutants. Direct or competitive enzyme-linked immunosorbent assays were established to detect ...
Phosphorylation of Thr654 but not Thr669 within the juxtamembrane domain of the EGF receptor inhibits calmodulin binding
Calcium-calmodulin (CaM) binding to the epidermal growth factor receptor (EGFR) has been shown to both inhibit and stimulate receptor activity. CaM binds to the intracellular juxtamembrane (JM) domain (Met645-Phe688) of EGFR. Protein kinase C (PKC) mediated phosphorylation of Thr654 occurs within this domain. CaM binding to the JM domain inhibits PKC phosphorylation and conversely PKC mediated phosphorylation of Thr654 or Glu substitution of Thr654 inhibits CaM binding. A second threonine residue (Thr669) within the JM domain is phosphorylated by the mitogen-activated protein kinase (MAPK). Previous results have shown that CaM interferes with EGFR-induced MAPK activation. If and how phosphorylation of Thr669 affects CaM-EGFR interaction is however not known.In the present study we have used surface plasmon resonance (BIAcore) to study the influence of Thr669 phosphorylation on real time interactions between the intracellular juxtamembrane (JM) domain of EGFR and CaM. The EGFR-JM was expressed as ...
Cancer-associated glycoforms of gelatinase B exhibit a decreased level of binding to galectin-3 - WestminsterResearch
Gelatinase B (MMP-9) and galectin-3 are widely known to participate in tumor cell invasion and metastasis. Glycans derived from MMP-9 expressed in MCF-7 breast cancer and THP-1 myeloid leukemia cells were compared with those from MMP-9 expressed in natural neutrophils. The many O-linked glycans of neutrophil gelatinase B presented a cluster of mainly galactosylated core II structures, 46% of which were ligands for galectin-3; 11% contained two to three N-acetyllactosamine repeating units that are high-affinity ligands for the lectin. The glycan epitopes thus provide MMP-9 with both high-affinity and (presumably) high-avidity interactions with galectin-3. In contrast, the O-glycans released from MMP-9 expressed in MCF-7 and THP-1 cells were predominantly sialylated core I structures. Only 10% of MCF-7 and THP-1 gelatinase B O-glycans were ligands for galectin-3 and contained only a maximum single N-acetyllactosamine repeat. Consistent with the glycan analysis, surface plasmon resonance binding ...
Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance SPR Technique: A Novel Method to Assess...
Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance SPR Technique: A Novel Method to Assess Biomolecular Interactions. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance
Several proteins, like transcription factors, bind to certain DNA sequences, thereby regulating biochemical pathways that determine the fate of the corresponding cell. Due to these key positions, it is indispensable to analyze protein-DNA interactions and to identify their mode of action. Surface plasmon resonance is a label-free method that facilitates the elucidation of real-time kinetics of biomolecular interactions. In this article, we focus on this biosensor-based method and provide a detailed guide how SPR can be utilized to study binding of proteins to oligonucleotides. After a description of the physical phenomenon and the instrumental realization including fiber-optic-based SPR and SPR imaging, we will continue with a survey of immobilization methods. Subsequently, we will focus on the optimization of the experiment, expose pitfalls, and introduce how data should be analyzed and published. Finally, we summarize several interesting publications of the last decades dealing with ...
Photothermal activation of astrocyte cells using localized surface plasmon resonance of gold nanorods - posted by Biophotonics...
Although it has been revealed that astrocytes, generally known as star-shaped glial cells, play critical roles in the functions of central nervous system, there have been few efforts to directly modul... Tags: intracellular calcium, near infrared, localized surface plasmon resonance, astrocytes, gold nanorods.
Evaluation of cytochrome c affinity to anionic phospholipids by means of surface plasmon resonance. | Sigma-Aldrich
Evaluation of cytochrome c affinity to anionic phospholipids by means of surface plasmon resonance. by German Stepanov, Oksana Gnedenko, Andrey Molnar, Alexis Ivanov, Yuri Vladimirov, Anatoly Osipov. FEBS letters. Read more related scholarly scientific articles and abstracts.
A chemical sensor based on surface plasmon resonance on surface modified optical fibers
A sensor is described which utilizes the phenomenon of surface plasmon resonance to detect changes in refractive index of chemical or biochemical samples applied to a surface modified optical fiber. The sensor is constructed by polishing a short section of the lateral surface of an optical fiber to its evanescent field surrounding the fiber core. One or more thin films are applied to the polished section of the fiber to produce the sensing element. One of the films is the metal silver, which acts as the support for the surface plasmon. Under the proper conditions, TM polarized energy propagating in the fiber can be coupled to a surface plasmon electromagnetic mode on the metal film. This coupling depends on the wavelength, the nature of the fiber, the refractive index and thickness of the thin films applied to the fiber, and the refractive index of a chemical sample in contact with the modified surface. The fiber to plasmon coupling is seen as a large attenuation of the light reaching the distal ...
Evaluation of an affinity-amplified immunoassay of graphene oxide using surface plasmon resonance biosensors<...
TY - GEN. T1 - Evaluation of an affinity-amplified immunoassay of graphene oxide using surface plasmon resonance biosensors. AU - Chiu, Nan-Fu. AU - Huang, Teng Yi. AU - Kuo, Chun Chuan. PY - 2015/1/1. Y1 - 2015/1/1. N2 - We describe a fundamental study on the plasmonic properties and advanced biosensing mechanisms of functionalized graphene. We discuss a specific design using modified carboxyl groups, which can modulate surface plasmon (SP) coupling and provide an advantage for their binding to the sensing layer with high-performance affinity in an immunological reaction. The functionalized graphene-based surface plasmon resonance (SPR) biosensors have three advantages: high performance, high sensitivity, and excellent molecular kinetic response. In the future, functionalized graphene sheets will make a unique contribution to photonic and SPR diagnosis devices. We wish to highlight the essential characteristics of functionalized graphene-based SPR biosensors to assist researchers in developing ...
Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors...
TY - JOUR. T1 - Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors to detect human chorionic gonadotropin. AU - Chiu, Nan Fu. AU - Kuo, Chia Tzu. AU - Lin, Ting Li. AU - Chang, Chia Chen. AU - Chen, Chen Yu. PY - 2017/8/15. Y1 - 2017/8/15. N2 - Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. We combined graphene oxide (GO) sheets with a specific peptide aptamer to create a novel, simple and label-free tool to detect abnormalities at an early stage of pregnancy, a GO-peptide-based surface plasmon resonance (SPR) biosensor. This is the first binding interface experiment to successfully demonstrate binding specificity in kinetic analysis biomechanics in peptide aptamers ...
An improved immunoassay for detection of saxitoxin by surface plasmon resonance biosensors<...
TY - JOUR. T1 - An improved immunoassay for detection of saxitoxin by surface plasmon resonance biosensors. AU - Yakes, B.J.. AU - Prezioso, S.. AU - Haughey, S.A.. AU - Campbell, Katrina. AU - Elliott, Christopher. AU - DeGrasse, S.L.. PY - 2011/8. Y1 - 2011/8. N2 - Saxitoxin and its analogs, the causative agents of paralytic shellfish poisoning (PSP), are a worldwide threat to seafood safety. Effective monitoring of potentially contaminated fishing areas as well as screening of seafood samples is necessary to adequately protect the public. While many analytical methods exist for detecting paralytic shellfish toxins (PSTs), each technique has challenges associated with routine use. One recently developed method  that overcomes ethical or performance-related issues of other techniques is the surface plasmon resonance (SPR) bioassay. Notwithstanding the advantages of this method, much research remains in optimizing the sensor substrate and assay conditions to create a robust technique for ...
Application of surface plasmon coupled emission to study of muscle<...
TY - JOUR. T1 - Application of surface plasmon coupled emission to study of muscle. AU - Borejdo, J.. AU - Gryczynski, Z.. AU - Calander, N.. AU - Muthu, P.. AU - Gryczynski, I.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2006/10. Y1 - 2006/10. N2 - Muscle contraction results from interactions between actin and myosin cross-bridges. Dynamics of this interaction may be quite different in contracting muscle than in vitro because of the molecular crowding. In addition, each cross-bridge of contracting muscle is in a different stage of its mechanochemical cycle, and so temporal measurements are time averages. To avoid complications related to crowding and averaging, it is necessary to follow time behavior of a single cross-bridge in muscle. To be able to do so, it is necessary to collect data from an extremely small volume (an attoliter, 10 -18 liter). We report here on a novel microscopic application of surface plasmon-coupled emission (SPCE), which provides such a ...
Surface plasmon resonance and emitted light properties of polystyrene sphere films<...
TY - JOUR. T1 - Surface plasmon resonance and emitted light properties of polystyrene sphere films. AU - Shinbo, Kazunari. AU - Miyabayashi, Syunsuke. AU - Yoshizawa, Kazushi. AU - Shimizu, Hidehiko. AU - Kato, Keizo. AU - Kaneko, Futao. AU - Tanaka, Masato. AU - Wakamatsu, Takashi. AU - Advincula, Rigoberto C.. PY - 2003. Y1 - 2003. N2 - Polystyrene sphere films were fabricated and the optical properties were investigated using surface plasmon spectroscopy (SPS) and emitted light due to reverse irradiation utilizing SPS Kretschmann configuration. The films were fabricated using sphere dispersed solution with various diameters from about 100 to 300 nm. The morphologies of the fabricated films were observed using atomic force microscopy and the sphere films had almost three layer structure. In SPS curve, large dip and shallow broad dip were observed at around 75° and 50°, respectively. The dips were considered to be due to the three layer structure and some defects in the film. Furthermore, ...
Specificity of Escherichia coli Heat-Labile Enterotoxin Investigated by Single-Site Mutagenesis and Crystallography[v2] |...
Diarrhoea caused by enterotoxigenic Escherichia coli is one of the leading causes of mortality in children under five years of age and is a great burden on developing countries. The major virulence factor of the bacterium is the heat-labile enterotoxin (LT), a close homologue of the cholera toxin. The toxins bind to carbohydrate receptors in the gastrointestinal tract, leading to toxin uptake and, ultimately, to severe diarrhoea. Previously, LT from human- and porcine-infecting ETEC (hLT and pLT, respectively) were shown to have different carbohydrate-binding specificities, in particular with respect to N-acetyllactosamine-terminating glycosphingolipids. Here, we probed eleven single-residue variants of the heat-labile enterotoxin with surface plasmon resonance spectroscopy and compared the data to the parent toxins. In addition we present a 1.45 Å crystal structure of pLTB in complex with branched Lacto-N-neohexaose (Galbeta4GlcNAcbeta6[Galbeta4GlcNAcbeta3]Galbeta4Glc). The largest difference in
The NSERC-CREATE Training Program in Integrated Sensor Systems | McGill Institute for Advanced Materials - McGill University
An integrated surface plasmon resonance sensor Welcome to the Integrated Sensor System Training Program Website. Sensing is inherently a multidisciplinary field, and those who seek to advance and exploit knowledge in this area have a wide range of backgrounds, including physics, chemistry, mechanical and electrical engineering, biomedical engineering and medicine. Although often well-trained in their own domain of expertise they are not always ready to transfer their skills to industry. Most students have little exposure to other related research domains and do not appreciate that sensors are complex systems, requiring biorecognition or chemical recognition elements, sample delivery components (e.g. microfluidics), transducing elements, signal amplification, signal processing and packaging, and that a multidisciplinary approach is essential. Sensor systems must address specific markets and are subject to regulatory control. Students often also lack knowledge in the key techniques of micro or nano
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Plural circuit selection using role reversing control inputs | SYSTEM AND METHOD OF DATA COMMUNICATION USING TRELLIS CODED MODULATION OR TURBO TRELLIS CODED MODULATION IN... | Multiple bit memory cells and methods for reading non-volatile data | Two-dimensional blazed MEMS grating | Surface plasmon resonance sensor having real-time referencing |
Asp271 is critical for substrate interaction with the surface binding site in β-agarase A from <i>Zobellia galactanivorans<...
TY - JOUR. T1 - Asp271 is critical for substrate interaction with the surface binding site in β-agarase A from Zobellia galactanivorans. AU - Wilkens, Casper. AU - Tiwari, Manish K.. AU - Webb, Helen. AU - Jam, Murielle. AU - Czjzek, Mirjam. AU - Svensson, Birte. PY - 2019. Y1 - 2019. N2 - In the marine environment agar degradation is assured by bacteria that contain large agarolytic systems with enzymes acting in various endo- and exo-modes. Agarase A (AgaA) is an endo-glycoside hydrolase of family 16 considered to initiate degradation of agarose. Agaro-oligosaccharide binding at a unique surface binding site (SBS) in AgaA from Zobellia galactanivorans was investigated by computational methods in conjunction with a structure/sequence guided approach of site-directed mutagenesis probed by surface plasmon resonance binding analysis of agaro-oligosaccharides of DP 4-10. The crystal structure has shown that agaro-octaose interacts via H-bonds and aromatic stacking along 7 subsites (L through R) of ...
The human low affinity Fcgamma receptors IIa, IIb, and III bind IgG with fast kinetics and distinct thermodynamic properties. -...
Fcgamma receptors (FcgammaRs) are expressed on all immunologically active cells. They bind the Fc portion of IgG, thereby triggering a range of immunological functions. We have used surface plasmon resonance to analyze the kinetic and thermodynamic properties of the interactions between the ectodomains of human low affinity FcgammaRs (FcgammaRIIa, FcgammaRIIb, and FcgammaRIIIb-NA2) and IgG1 or the Fc fragment of IgG1. All three receptors bind Fc or IgG with similarly low affinities (K(D) approximately 0.6-2.5 microm) and fast kinetics, suggesting that FcgammaR-mediated recognition of aggregated IgG and IgG-coated particles or cells is mechanistically similar to cell-cell recognition. Interestingly, the Fc receptors exhibit distinct thermodynamic properties. Whereas the binding of the FcgammaRIIa and FcgammaRIIb to Fc is driven by favorable entropic and enthalpic changes, the binding of FcgammaRIII is characterized by highly unfavorable entropic changes. Although the structural bases for these
Investigation of the mechanism of binding between internalin B and heparin using surface plasmon resonance<...
TY - JOUR. T1 - Investigation of the mechanism of binding between internalin B and heparin using surface plasmon resonance. AU - Hrtska, Sybil C Lang. AU - Kemp, Melissa M.. AU - Muñoz, Eva M.. AU - Azizad, Omaira. AU - Banerjee, Mani. AU - Raposo, Catarina. AU - Kumaran, Jyothi. AU - Ghosh, Partho. AU - Linhardt, Robert J.. PY - 2007/3/13. Y1 - 2007/3/13. N2 - Listeria monocytogenes, a food-borne pathogen that infects immunocompromised patients, enters and proliferates within mammalian cells by taking advantage of host cell machinery. While entry into macrophages and other phagocytic cells occurs constitutively, intracellular invasion of nonphagocytic cells, such as epithelial and endothelial cells, occurs through induced phagocytosis. Invasion of these nonphagocytic cell types is under the control of the secreted L. monocytogenes protein internalin B (InlB), which directly associates with and activates the receptor tyrosine kinase Met. Activation of Met by InlB has previously been shown to be ...
Biosensor technology addressing agricultural problems - Open Research Online
Biosensor technology is a powerful alternative to conventional techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diaberics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate ...
Topographically Flat Nanoplasmonic Sensor Chips for Biosensing and Materials Science
Nanoplasmonic sensors typically comprise arrangements of noble metal nanoparticles on a dielectric support. Thus they are intrinsically characterized by surface topography with corrugations at the 10-100 nm length scale. While irrelevant in some bio- and chemosensing applications, it is also to be expected that the surface topography significantly influences the interaction between solids, fluids, nanoparticles and (bio)molecules, and the nanoplasmonic sensor surface. To address this issue, we present a wafer-scale nanolithography-based fabrication approach for high-temperature compatible, chemically inert and topographically flat and laterally homogeneous nanoplasmonic sensor chips. We demonstrate their sensing performance on three different examples, for which we also carry out a direct comparison with a traditional nanoplasmonic sensor with representative surface corrugation. Specifically, we (i) quantify the film-thickness dependence of the glass transition temperature in poly(methyl metacrylate)
Sensors | Free Full-Text | Low Cost Sensors Based on SPR in a Plastic Optical Fiber for Biosensor Implementation | HTML
This paper reports the fabrication and testing of two configurations of optical sensor systems based on Surface Plasmon Resonance (SPR) at the interface of a liquid sample and sandwiched structures realized starting from the exposed core of a Plastic Optical Fiber (POF). The proposed geometries have proven to be suitable for measuring the refractive indexes of liquids whose refractive index falls around 1.35. Furthermore, the proposed sensing head, being low cost and relatively easy to realize, may be very attractive for biosensor implementation.
Interaction of the coiled-coil domain with glycosaminoglycans protects angiopoietin-like 4 from proteolysis and regulates its...
Angiopoietin-like 4 (ANGPTL4) is involved in angiogenesis and lipid metabolism. It is secreted by liver and adipose tissues and cleaved to generate circulating coiled-coil domain (CCD) and fibrinogen-like domain (FLD) fragments. The full-length ANGPTL4 produced by hypoxic endothelial cells interacts with the extracellular matrix (ECM). The ECM-bound and soluble forms of ANGPTL4 have antiangiogenic properties. We carried out a structure-function analysis to investigate the regulation of ANGPTL4 bioactivity in endothelial cells. We found that the recombinant CCD binds to the ECM, whereas the FLD is released into the medium. The CCD, like the full-length ANGPTL4, binds to heparan and dermatan sulfates in surface plasmon resonance assays and inhibits endothelial cell adhesion, motility, and tubule-like formation. In endothelial cells, ANGPTL4 is processed in the secretion medium after release from the ECM. This processing is altered by the proprotein convertases inhibitor alpha1-PDX and abolished by the
Optimized Preparation of Silver Nanoparticles from Polyethylene Glycol and Formaldehyde
The purpose of this work was to study the effect of various process and formulation parameters on size and shapes of silver nanoparticles (AgNPs) based on surface plasmon resonance (SPR). AgNPs were prepared by chemical reduction using formaldehyde (HCHO) as reducing agent and capped by polyethylene glycol (PEG). Effect of several processing variables including the concentration and volume of capping agent and reaction time is reported. The size of monodispersed nanoparticles was between 30-100 nm and was stable for three months at both room temperature and 4°C ...
Sensitive and label-free biosensing of RNA with predicted secondary structures by a triplex affinity capture method
A novel biosensing approach for the label-free detection of nucleic acid sequences of short and large lengths has been implemented, with special emphasis on targeting RNA sequences with secondary structures. The approach is based on selecting 8-aminoadenine-modified parallel-stranded DNA tail-clamps as affinity bioreceptors. These receptors have the ability of creating a stable triplex-stranded helix at neutral pH upon hybridization with the nucleic acid target. A surface plasmon resonance biosensor has been used for the detection. With this strategy, we have detected short DNA sequences (32-mer) and purified RNA (103-mer) at the femtomol level in a few minutes in an easy and level-free way. This approach is particularly suitable for the detection of RNA molecules with predicted secondary structures, reaching a limit of detection of 50fmol without any label or amplification steps. Our methodology has shown a marked enhancement for the detection (18 for short DNA and 54 for RNA), when compared ...
OpenPlex Research Platform | HORIBA Scientific | Jul 2013 | Photonics.com
OpenPlex, Horiba Scientifics redesigned manual label-free SPRi-Lab+ system, uses surface plasmon resonance imaging (SPRi) for real-time analysi
Better biosensor technology created for stem cells | EurekAlert! Science News
A Rutgers-led team has created better biosensor technology that may help lead to safe stem cell therapies for treating Alzheimers and Parkinsons diseases and other neurological disorders. The technology, which features a unique graphene and gold-based platform and high-tech imaging, monitors the fate of stem cells by detecting genetic material (RNA) involved in turning such cells into brain cells (neurons), according to a study in the journal Nano Letters.
Correlating Plasmon Resonance and Raman Scattering
Nanoplasmonics has raised much concern on tailoring the strong optical fields near noble metal nanostructures. In particular, surface-enhanced Raman Scattering (SERS) can take the full benefits of nanoplasmonics for providing one of the most highly sensitive biochemical sensing techniques. This sensitivity is used to identify biochemical molecules even at single molecule level. This technique does not require fluorescent labeling. For decades, thousands of plasmonic nanoparticles or nanostructures mounted on substrates have been reported for plasmonics-driven SERS. However, previous work overlooks consideration of the quantitative investigation between plasmon resonance and SERS signals. This oversight has been due to available methodology.. Universal correlations between plasmon resonance and SERS has recently been described by the research of Ki-Hun Jeong (see, Kang et al.1) in Journal Advanced Materials - This observation was successfully enabled by deformable nanoplasmonic membrane, i.e., ...
Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions. - MRC Weatherall...
The αβ T-cell coreceptor CD4 enhances immune responses more than 1 million-fold in some assays, and yet the affinity of CD4 for its ligand, peptide-major histocompatibility class II (pMHC II) on antigen-presenting cells, is so weak that it was previously unquantifiable. Here, we report that a soluble form of CD4 failed to bind detectably to pMHC II in surface plasmon resonance-based assays, establishing a new upper limit for the solution affinity at 2.5 mM. However, when presented multivalently on magnetic beads, soluble CD4 bound pMHC II-expressing B cells, confirming that it is active and allowing mapping of the native coreceptor binding site on pMHC II. Whereas binding was undetectable in solution, the affinity of the CD4/pMHC II interaction could be measured in 2D using CD4- and adhesion molecule-functionalized, supported lipid bilayers, yielding a 2D Kd of ∼5,000 molecules/μm(2) This value is two to three orders of magnitude higher than previously measured 2D Kd values for interacting
OPUS Würzburg | Search
The obligate human pathogen Neisseria gonorrhoeae is responsible for the widespread sexually transmitted disease gonorrhoea, which in rare cases also leads to the development of disseminated gonococcal infection (DGI). DGI is mediated by PorBIA-expressing bacteria that invade host cells under low phosphate condition by interaction with the scavenger receptor-1 (SREC-I) expressed on the surface of endothelial cells. The interaction of PorBIA and SREC-I was analysed using different in vitro approaches, including surface plasmon resonance experiments that revealed a direct phosphate-independent high affinity interaction of SREC-I to PorBIA. However, the same binding affinity was also found for the other allele PorBIB, which indicates unspecific binding and suggests that the applied methods were unsuitable for this interaction analysis. Since N. gonorrhoeae was recently classified as a super-bug due to a rising number of antibiotic-resistant strains, this study aimed to discover inhibitors against ...
DSpace at KIST: Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor
Generally, an immunoaffinity SPR biosensor detects a target analyte in a sample through highly selective adsorption by using the antigen–antibody interaction. For improving the sensitivity, various kinds of particles have been added to the already bound analytes on the SPR biosensor (sandwich assay). In this work, signal amplification was demonstrated by the expression of the IgG-binding Z-domain of protein A on the outer membrane of Escherichia coli via Autodisplay. The amount of Z-domain of protein A expressed on the outer membrane was calculated to be 280,000 molecules per cell. In addition, the IgGbinding ability of the expressed proteinwas characterized using FACS analysis. The signal amplification of the SPR biosensor was performed in the sandwich assay format using a model of horseradish peroxidase (HRP); the limit of detectionwas determined to be significantly improved from1 g/ml to 1 ng/ml. Finally, myoglobin analysis was demonstrated for the medical diagnosis of cardiac ...
Monitoring of influenza virus hemagglutinin in process samples using weak affinity ligands and surface plasmon resonance
Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.. A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.. When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.. The design of a sensor surface is important for the characteristics of a sensor. By binding ...
Au Nanoparticle Coupled- Surface Plasmon Resonance Immuno-sensor for Sensitivity Enhancement
In order to overcome the sensitivity limitation of SPR, nanoparticle-coupled SPR biosensors have explored since nanoparticles may significantly enhance the sensitivity by 1-2 orders of magnitude. Although the nanoparticle conjugated method contributes to the enhancement of the sensitivity, it does not take an advantage of SPR from the viewpoint of label-free detection. Herein we demonstrate the sensitivity enhancement with Au nanoparticles coupled- SPR immuno-sensor chip on which the specific size and surface density of the particles controlled as a label-free detection system. Au nanopariticles were synthesized and selected with a specific size. Surface density of immobilized 30 nm Au nanoparticles on bare Au film was estimated as 1x109 ea/cm2 using atomic force microscopy. With systematic control of the size of the Au nanoparticle and thickness of the bare Au film, it was found that 30 nm Au particles on 50 nm thick Au film demonstrated the largest resonance angle shift for surface reaction on the
Toward surface plasmon polariton quantum-state tomography<...
TY - JOUR. T1 - Toward surface plasmon polariton quantum-state tomography. AU - Dominguez, D.. AU - Regan, C. J.. AU - Bernussi, A. A.. AU - Grave De Peralta, L.. PY - 2013/2/21. Y1 - 2013/2/21. N2 - We report the direct excitation and detection of single-photon surface plasmon polariton (SPP) using a SPP tomography arrangement. Temporally spaced photons produced by spontaneous parametric downconversion were used to excite single-photon SPPs. The quantum statistics of the leakage radiation was studied using a Hanbury-Brown Twiss correlator arrangement. We observed a violation of the second order coherence test indicating leakage of temporally spaced photons. This demonstrates that leakage radiation associated with SPPs excited by single photons is composed of temporally spaced photons. Reaching the quantum regime of SPP tomography opens the door for further advances in SPP quantum state determination using SPP tomography.. AB - We report the direct excitation and detection of single-photon ...
Real-Time Surface Plasmon Resonance Imaging Measurements for the Multiplexed Determination of Protein Adsorption/Desorption...
Calibration of Surface Plasmon Resonance Imager for Biochemical Detection
International Journal of Electrochemistry is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles that further our understanding of fundamental electrochemical processes, describe new electrochemical techniques, apply electrochemistry in analytical determination, or apply electrochemistry for chemical reactivity.
Phys.org - surface plasmon resonance
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nRF51802 Bluetooth 4.0 Temperature Atmospheric Acceleration Sensor Mod - diymore
Product Introduction:Features:1.Adopt nRF51822 bluetooth 4.0 BLE SOC 2.ARM CORTEX-M0 3.Adopt MPU6050 chip 4.Adopt BMP180 temperature atmospheric pressure sensor chip 5.Ambient light proximity sensor chip AP32166.With power switch 7.Support CR2032 button battey to supply power 8.Size:30mm*30mm Package Included: 1 x
Narrow plasmon resonances in hybrid systems (eBook, 2018) [WorldCat.org]
Get this from a library! Narrow plasmon resonances in hybrid systems. [Philip A Thomas] -- Advances in understanding the interactions between light and subwavelength materials have enabled the author and his collaborators to tailor unique optical responses at the nanoscale. In particular, ...
Real-time biomolecular interaction analysis: novel applications and developments -...
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Surface-plasmon Resonance (SPR) Sensing - NSF-REU
Mentors: Berron, Hastings. Surface-plasmon resonance (SPR) sensing is a widely used optical technique for detecting and analyzing biochemical interactions. It has found applications in diverse fields such as medical diagnostics, drug discovery, and environmental monitoring. A key challenge for SPR is maximizing sensitivity to a target analyte while minimizing response to interfering species. Dr. Hastings group has developed several SPR sensors that use optical methods to distinguish specific and non-specific interactions. Dr. Berrons group has developed stable, low-density self-assembled monolayers that are ideal for functionalizing the gold surfaces common to SPR sensors. The tunable density of exposed functional groups should allow optimization of the interaction with a chosen target, while suppressing non-specific protein binding. In addition, the SPR technique itself will increase our understanding of the kinetics of protein adsorption on low-density monolayers. Students working on this ...
The interaction of serum albumin with cholesterol containing lipid vesicles<...
TY - JOUR. T1 - The interaction of serum albumin with cholesterol containing lipid vesicles. AU - Meierhofer, T. AU - van den Elsen, J M H. AU - Cameron, Petra J. AU - Munoz-Berbel, X. AU - Jenkins, A T A. PY - 2010/1. Y1 - 2010/1. N2 - In this paper, the interaction of both human blood serum (the primary fraction of which is serum albumin) and pure human serum albumin (HSA) with surface immobilised lipid vesicles was measured by combined Surface Plasmon Resonance (SPR) and Surface Plasmon enhanced Fluorescence (SPEFS), and fluorescence microscopy. It was found that both blood serum and HSA showed specific binding to vesicles which contained cholesterol, resulting in increased membrane permeability and release of encapsulated fluorescent dye. This effect was not seen with heat inactivated blood serum, heat inactivated HSA or in vesicles not containing cholesterol. These results suggest that HSA may have a physiological role over and beyond that of fatty acid carrier, possibly acting to regulate ...
Biochips in Drug Development
Biomolecular interaction analysis (BIA) using SPR (surface plasmon resonance) biosensors is now utilised increasingly in nearly all phases of drug development. The BIA system consists out of a light source emitting near infrared light, a sensor microchip, an automated liquid handling system with constant flow and a diode array position-sensitive detector. One of the two interacting partners (referred to as the ligand) is immobilized on the sensor surface. The other binding partner, called the analyte, is directed over the surface in a constant flow system allowing to monitor the interaction of the binding partners in real time ...
UMR UNIS 1072 El far Debanne Goaillard Seagar Inserm neurobiologie hom ostasie excitabilit neuronale
The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon resonance (SPR) that is more sensitive than the standard mouse bioassay for the quantification of BoNT/A activity. We have now adapted this procedure for pharmaceutical preparations. The optimized SPR assay allowed multiple measurements on a single chip, including the kinetics of substrate cleavage. The activity of five different batches of a pharmaceutical BoNT/A preparation was determined in a blind study by SPR and found to be in agreement with data from the in vivo mouse lethality assay. Biosensor detection of specific proteolytic products has the potential to accurately monitor the activity of pharmaceutical BoNT/A preparations, and a single chip can be used to assay more than 100 ...
Protein Interaction Analysis Services - Profacgen
Profacgen provides One-Stop-Service on protein-protein interaction analysis, including Yeast two-hybrid, Pull-downs and Surface Plasmon Resonance (SPR) assay etc., to facilitate your scientific research. Our service can be tailored according to your specific requirements.
Alexander Barnes | Department of Chemistry
Our laboratory improves magnetic resonance technology and spectroscopy for biomolecular structure determination relevant to HIV cure research. Solid state nuclear magnetic resonance (NMR) is exquisitely suited to probe atomic level structural detail of biomolecules within intact human cells. However, the small nuclear magnetic moments that yield narrow resonances also result in very low inherent sensitivity in NMR. Dynamic Nuclear Polarization (DNP) is a powerful combined electron paramagnetic resonance (EPR) and NMR technique that transfers the strong polarization from unpaired electron spins to nuclear spins to boost NMR sensitivity. In our DNP experiments, we employ frequency agile gyrotrons, and extreme cryogenic sample cooling to increase the sensitivity of solid state NMR experiments up to a factor of 20,000. This tremendous gain in sensitivity results in acquiring data 400 million times faster than conventional NMR experiments and will have a profound impact on magnetic resonance ...
Nova Biomedical : World Leader in Biosensor Technology
Waltham, MA, January 2011 - Nova Biomedical today announced that, in response to rapid growth in its diabetes and whole blood point-of-care testing products business, it has purchased an additional 80,000 square-foot manufacturing/warehouse facility in Billerica, MA. According to Lou Borrelli, Nova Biomedical CFO, This additional state of the art manufacturing facility will ensure that our manufacturing capabilities keep pace with the increasing demand for our StatStrip Hospital Glucose products as well as our Nova Max consumer diabetes products.. One of the main drivers for Novas strong growth is the rapid adoption of its StatStrip Hospital Glucose Monitoring System. Since its inception just four years ago, StatStrip has become the fastest growing hospital glucose meter in the world. StatStrip uses a novel glucose test strip technology that measures hematocrit and other common interferences such as maltose, galactose, xylose, acetaminophen, ascorbic acid and oxygen, and eliminates erroneous ...
Advances in Biosensor Technology Could Limit the Spread of HIV | IDTechEx Research Article
The most rapid and reliable way of detecting HIV is to search for the HIV genetic material directly in blood, using a process called PCR (polymerase chain reaction). This has the advantage of not needing to wait for such a long time after exposure, being used after only 3 days. Traditionally this process, known as molecular diagnostics, has been performed in a laboratory by trained personnel. Recent technological advances have however brought the possibility of conducting molecular diagnostics out of the laboratory and next to the patient at the point-of-care ...
Surface plasmon resonance
Hydrogen sensor Multi-parametric surface plasmon resonance Nano-optics Plasmon Spinplasmonics Surface plasmon polariton Waves ... supporting surface electromagnetic waves with sharper resonances (Bloch surface waves). If the surface is patterned with ... In multi-parametric surface plasmon resonance, two SPR curves are acquired by scanning a range of angles at two different ... Multi-parametric surface plasmon resonance, a special configuration of SPR, can be used to characterize layers and stacks of ...
Surface plasmon resonance microscopy
The surface plasmon resonance microscopy is based on surface plasmon resonance and recording desired images of the structures ... Surface plasmon resonance microscopy (SPRM), also called surface plasmon resonance imaging (SPRI), is a label free analytical ... Handbook of Surface Plasmon Resonance: Surface Plasmon Fluorescence Techniques for Bioaffinity Studies. RSC Publishing. Baba, A ... Tang, Y; Zeng, X; Liang, J (2010). "Surface Plasmon Resonance: An Introduction of a Surface Spectroscopy Technique". Journal of ...
Multi-parametric surface plasmon resonance
... (MP-SPR) is based on surface plasmon resonance (SPR), an established real-time label ... Jussila, Henri; Yang, He; Granqvist, Niko; Sun, Zhipei (5 February 2016). "Surface plasmon resonance for characterization of ... "Multispectral surface plasmon resonance approach for ultra-thin silver layer characterization: Application to top-emitting OLED ... "Monitoring of drug release kinetics from thin polymer films by multi-parametric surface plasmon resonance". International ...
Alongside Surface Plasmon Resonance, BLI is one of few widely available label-free biosensing technologies, a detection style ... Handbook of Surface Plasmon Resonance. Royal Society of Chemistry. ISBN 978-1-78801-139-6. OCLC 988866146. Syahir A, Usui K, ... This essentially creates a thin-film interference, in which the created layer acts as a thin film bound by these two surfaces. ... White light from a tungsten lamp is shone onto the biosensor tip and reflected off both surfaces, creating two unique ...
Localized surface plasmon
Surface plasmon resonance can also be tuned based on the shape of the nanoparticle. The plasmon frequency can be related to the ... Localized surface plasmons are distinct from propagating surface plasmons. In localized surface plasmons, the electron cloud ... Surface plasmon resonance Surface-enhanced Raman spectroscopy Nanoparticle Tip-enhanced Raman spectroscopy Kelly, K. Lance ( ... A localized surface plasmon (LSP) is the result of the confinement of a surface plasmon in a nanoparticle of size comparable to ...
Other methods include surface plasmon resonance (SPR), protein microarrays, dual polarisation interferometry, microscale ... Visser, NF; Heck, AJ (June 2008). "Surface plasmon resonance mass spectrometry in proteomics". Expert Review of Proteomics. 5 ( ... de Mol, NJ (2012). "Surface plasmon resonance for proteomics". Chemical Genomics and Proteomics. Methods in Molecular Biology. ...
Surface plasmon polariton
Surface plasmon Surface plasmon resonance Localized surface plasmon Plasmonic lens Superlens Graphene plasmonics Surface wave ... Surface plasmon-based circuits, including both SPPs and localized plasmon resonances, have been proposed as a means of ... The term "surface plasmon polariton" explains that the wave involves both charge motion in the metal ("surface plasmon") and ... Pitarke, J M; Silkin, V M; Chulkov, E V; Echenique, P M (2007). "Theory of surface plasmons and surface-plasmon polaritons" ( ...
... its linear optical response is mainly controlled by the surface plasmon resonance (SPR). Surface plasmon resonance occurs when ... Platinum NPs synthesized via citrate reduction do not have a surface plasmon resonance peak around 215 nm. Through ... Mayer, K. M; Hafner, J. H (2011). "Localized surface plasmon resonance sensors". Chemical Reviews. 111 (6): 3828-57. doi: ... Willets, Katherine A; Van Duyne, Richard P (2007). "Localized Surface Plasmon Resonance Spectroscopy and Sensing". Annual ...
... which generates localized surface plasmon resonances (LSPRs) when excited by light. These plasmon oscillations create an ... Amendola, V.; Pilot, R.; Frasconi, M.; Marago, O. M.; Iati, M. A. Surface plasmon resonance in gold nanoparticles: a review. J ... Homola, J.; Yee, S. S.; Gauglitz, G. Surface plasmon resonance sensors: review. Sens. Actuators, B 1999, 54, 3−15. Zhang, Y.; ... Their ability to utilize energy from near-infrared, visible, and ultraviolet light gives plasmon surfaces higher light- ...
Schasfoort, R.B.M. (2017). Handbook of Surface Plasmon Resonance (2nd ed.). Royal Society of Chemistry. p. 296. ISBN ... The steps are: A surface is prepared with a known quantity of capture antibody. Any nonspecific binding sites on the surface ... The surface of the Scavenger container or channel has "Scavenger Antigens" bound to it. These can be identical or sufficiently ... In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody ...
"Weak value amplification of an off-resonance Goos-Hänchen shift in a Kretschmann-Raether surface plasmon resonance device". ... In order to generate a giant Goos-Hänchen shift up to 100 μm, surface plasmon resonance techniques were applied based on an ... X. Yin (2006). "Goos-Hänchen shift surface plasmon resonance sensor". Applied Physics Letters. 89 (26): 261108. Bibcode: ... Both waves are reflected from the surface and undergo different phase shifts, which leads to a lateral shift of the finite beam ...
Laroy W, Ameloot P, Contreras R (2001). "Characterization of sialyltransferase mutants using surface plasmon resonance". ... 1990). "The B cell antigen CD75 is a cell surface sialytransferase". J. Exp. Med. 172 (2): 641-3. doi:10.1084/jem.172.2.641. ... 1992). "The HB-6, CDw75, and CD76 differentiation antigens are unique cell-surface carbohydrate determinants generated by the ... is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76 ...
NNV Najaarsvergadering 2013 Iu, H.; Li, J.; Ong, H. C.; Wan, J. T. K. (2008). "Surface plasmon resonance in two-dimensional ... and their inner surface was oxidized in the process. Then the Al matrix, but not the oxidized aluminium, was etched away by the ...
... such that binding of a target analyte to a receptor on the gold surface produces a measurable signal. Surface plasmon resonance ... 2007). "A localized surface plasmon resonance based immunosensor for the detection of casein in milk". Sci. Technol. Adv. Mater ... 2014). "Nanomaterials enhanced surface plasmon resonance for biological and chemical sensing applications" (PDF). Chemical ... Homola J (2003). "Present and future of surface plasmon resonance biosensors". Anal. Bioanal. Chem. 377 (3): 528-539. doi: ...
Nanoparticle interfacial layer
"Surface plasmon resonance in gold nanoparticles: a review". Journal of Physics: Condensed Matter. 29 (20): 203002. doi:10.1088/ ... is that dense monolayers that consist of certain specific short chain ligands tend to dampen the surface plasmon resonance ... "Solvent and Ligand Effects on the Localized Surface Plasmon Resonance (LSPR) of Gold Colloids". The Journal of Physical ... Plasmon resonance can be used to analyze the surfactants of the nanoparticle. This principle is based on the so-called Fröhlich ...
Complement component 3
"Complement C3b interactions studied with surface plasmon resonance technique". International Immunopharmacology. 1 (3): 495-506 ... it exposes a reactive thioester that allows the peptide to covalently attach to any surface that can provide a nucleophile such ...
March 2001). "Complement C3b interactions studied with surface plasmon resonance technique". International Immunopharmacology. ... on pathogen surfaces ensuring that self surfaces are protected whilst complement proceeds unabated on foreign surfaces. The ... Factor H exerts its protective action on self cells and self surfaces but not on the surfaces of bacteria or viruses. This is ... Factor H regulates complement activation on self cells and surfaces by possessing both cofactor activity for the Factor I ...
"Dynamically Modulating the Surface Plasmon Resonance of Doped Semiconductor Nanocrystals". Nano Letters. 11 (10): 4415-4420. ... "Dynamically Modulating the Surface Plasmon Resonance of Doped Semiconductor Nanocrystals". Nano Letters. 11 (10): 4415-4420. ... "Exceptionally Mild Reactive Stripping of Native Ligands from Nanocrystal Surfaces by Using Meerwein's Salt". Angewandte Chemie ...
Another method to be considered is Surface Plasmon Resonance (SPR). SPR can detect binding of label free molecules on the ... These resins have ligands attached to their surfaces which are specific for the compounds to be separated. Most frequently, ... allowing for separation of proteins based on their surface hydrophobicity. Target proteins and their product aggregate species ... surface of a chip. If the desired protein is an antibody, binding can be translated directly to the activity of the protein. ...
Scanning probe lithography
Chen, Hsiang-An; Lin, Hsin-Yu; Lin, Heh-Nan (June 17, 2010). "Localized Surface Plasmon Resonance in Lithographically ... uses a heatable scanning probe in order to efficiently remove material from a surface without the application of significant ... or according to the driving mechanisms of the probe-surface interaction used in the patterning process: mechanical, thermal, ... Transitioning from Field Induced Oxidation to Solvent Decomposition through Surface Modification". Advanced Materials. 19 (21 ...
Complement component 4B
Laich A, Sim RB (Jan 2001). "Complement C4bC2 complex formation: an investigation by surface plasmon resonance". Biochimica et ... The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha ...
Organic photorefractive materials
Oh, J.; Choi, J. (2009). "Tunable color filter with surface plasmon resonance using organic photorefractive composite". Applied ... leads to the absorption of wavelengths generated by surface plasmon resonance and the reflection of complementary wavelengths. ...
Çimen, Duygu; Bereli, Nilay; Denizli, Adil (2022-06-01). "Patulin Imprinted Nanoparticles Decorated Surface Plasmon Resonance ... "Patulin Imprinted Nanoparticles Decorated Surface Plasmon Resonance Chips for Patulin Detection". Photonic Sensors. 12 (2): 117 ...
Ligand binding assay
Surface Plasmon Resonance (SPR) does not require labeling of the ligand. Instead, it works by measuring the change in the angle ... and surface plasmon resonance (SPR). In order to measure process of ligand-receptor binding, most non-radioactive methods ... Fluorescence Resonance Energy Transfer (FRET) utilizes energy transferred between the donor and the acceptor molecules that are ... Soaking the filter in a solution that would give the filter a positive surface charge would attract the negatively charged ...
S. Zeng; Baillargeat, Dominique; Ho, Ho-Pui; Yong, Ken-Tye (2014). "Nanomaterials enhanced surface plasmon resonance for ... The surface plasmon polariton (SPP) is an electromagnetic surface wave that can travel along an interface between two media ... Surface waves are caused when P waves and S waves come to the surface. Examples are the waves at the surface of water and air ( ... Other types of surface wave are the trapped surface wave, the gliding wave and Dyakonov surface waves (DSW) propagating at the ...
Zeng, S.; Baillargeat, Dominique; Ho, Ho-Pui; Yong, Ken-Tye (2014). "Nanomaterials enhanced surface plasmon resonance for ... Size-dependent properties are observed such as quantum confinement in semiconductor particles, surface plasmon resonance in ... Topically applied surface-enhanced resonance Raman ratiometric spectroscopy (TAS3RS)- TAS3RS is another technique that is ... There was a study that was done where two different surface enhanced resonance Raman scattering were synthesized (SERRS). One ...
... to be easily converted into an anti-fouling surface. Surface plasmon resonance (SPR) sensors are thin film-refractometers that ... Homola, Jiří (2008). "Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species". Chemical Reviews. ... High surface energies cause adsorption because a contaminated surface will have a smaller difference between the surface and ... Bondi, A. (1953). "The Spreading of Liquid Metals on Solid Surfaces. Surface Chemistry of High-Energy Surfaces". Chemical ...
"Measurement of small displacement based on surface plasmon resonance heterodyne interferometry". Optics and Lasers in ... "Common-path spectral interferometry with temporal carrier for highly sensitive surface plasmon resonance sensing". Optics ... "Testing Curved Surfaces and Lenses" (PDF). University of Arizona College of Optical Sciences. Archived from the original (PDF) ... It is normally used without any coating on either surface, so as to minimize ghost reflections. An aberrated wavefront from a ...
... characterization with surface plasmon resonance". Biological Chemistry. 381 (3): 265-268. doi:10.1515/BC.2000.034. PMID ...
Contrary to refractometric methods for label-free biomolecular interaction analysis, such as surface plasmon resonance (SPR) ... "Biomolecular Interaction Analysis in Drug Discovery Using Surface Plasmon Resonance Technology". Current Pharmaceutical Design ... Subsequent surface chemistry steps enable an easy tailoring of recognition molecules specific to the desired analytical ... Therefore, even in the absence of recognition elements the off-target molecules readily adsorb to the surface of the sensor. ...
For metal particles, the peak visible in the scattering cross-section is also called localized plasmon resonance. In the limit ... This can be seen as an extension to a spherical surface of Giles' and Wild's results for reflection at a planar surface with ... The existence of resonances and other features of Mie scattering makes it a particularly useful formalism when using scattered ... Mie resonance mediated light diffusion and random lasing. Mie solution for spherical particles. PyMieScatt, a Mie solution ...
Sandra J. Rosenthal
"Novel Synthesis of Chalcopyrite CuxInyS2 Quantum Dots with Tunable Localized Surface Plasmon Resonances". Chemistry of ...
Index of biochemistry articles
... surface immunoglobulin - surface plasmon resonance - suspension (chemistry) - synapse - synthetic vaccine - systems biology T ... nuclear magnetic resonance - NMR - nuclear protein - nucleic acid - nucleic acid regulatory sequence - nucleic acid repetitive ... protein nuclear magnetic resonance spectroscopy - protein P16 - protein P34cdc2 - protein precursor - protein structure ... cell surface receptor - cellular respiration - cellulose - centriole - centromere - centrosome - chaperone - chelation - ...
Vibrational analysis with scanning probe microscopy
This technique is based on surface plasmon resonance (SPR) which is the precursor of tip-enhanced Raman scattering (TERS) and ... Both TERS and SERS rely on a localized surface plasmon for increasing the ought-to-be weak Raman signal. The only difference ... parallel to the surface normal) incidence light, the plasmon excitation at the tip is most efficient. If the focusing objective ... If it is parallel (p-polarized) to the tip axis, the free electrons on the surface of the metal are confined to the end of the ...
Graphene also accommodates surface plasmons, observed via near field infrared optical microscopy techniques and infrared ... negative refraction and enhanced plasmon resonance effects, enabled by their unique optical properties. These specific ... They may enable the development of optical sensors that exploit the confinement of surface plasmons within a certain type of ... To create this response, incident light couples with the undulating, gas-like charges (plasmons) normally on the surface of ...
"Real time kinetic studies of the interaction between folded antisense and target RNAs using surface plasmon resonance". J Mol ...
Ravindra Kumar Sinha (physicist)
Here, it is shown that negative index is achieved by excitation of Surface Plasmon Polariton waves operating in dispersion ... Dillu, Venus; Sinha, R. K. (2013). "Enhanced Fano resonance in silver ellipsoidal plasmonic crystal cavity". Journal of Applied ... Dillu, Venus; Sinha, R. K. (2013). "Surface Plasmon Polariton Band Gap-Enabled Plasmonic Mach-Zehnder Interferometer: Design, ... Plasmonics & Plasmonic Bandgap Engineering: Surface Plasmon Polaritons (SPPs) are electromagnetic waves guided along metal ...
Robert W. Wood
This unusual phenomenon was named Wood's anomaly and led to the discovery of the surface plasmon polariton (SPP), a particular ... Resonance radiation and resonance spectra. New York: Columbia Univ. Press. ISBN 978-1-171-56302-0. Train, A. C. & Wood, R. W. ( ... When a glass rod was pressed lightly against a glass plate it etched the surface, while if pressed harder it bored right ... higher than the rest of the surface of the oil. At lower powers, the mound was low and lumpy; at high powers, it would rise up ...
Fragment-based lead discovery
... surface plasmon resonance (SPR), isothermal titration calorimetry (ITC) and Microscale Thermophoresis (MST) are routinely-used ... In particular, ligand-observe nuclear magnetic resonance (NMR) methods such as water-ligand observed via gradient spectroscopy ...
Fourth Industrial Revolution
Sensitivity and detection limit of concentration and adsorption measurements by laser-induced surface-plasmon resonance, Appl. ...
Potential applications of graphene
The wavelength of surface plasmon polaritons for a given frequency is several hundred times smaller than the wavelength of ... and cytotoxicity of a novel graphene-based multimodal magnetic resonance imaging-X-ray computed tomography contrast agent". J. ... thereby forming surface plasmon polaritons (SPP). When the antenna becomes resonant (an integral number of SPP wavelengths fit ... Its surface is covered with epoxy, hydroxyl, and carboxyl groups that interact with cations and anions. It is soluble in water ...
... nuclear magnetic resonance, x-ray crystallography, surface plasmon resonance, electron paramagnetic resonance, cross-linking ...
Noble metal thin films are used in plasmonic structures such as surface plasmon resonance (SPR) sensors. Surface plasmon ... Serrano, A.; Rodríguez de la Fuente, O.; García, M. A. (2010). "Extended and localized surface plasmons in annealed Au films on ... Liu, Liu; Han, Zhanghua; He, Sailing (2005). "Novel surface plasmon waveguide for high integration". Optics Express. 13 (17): ... Surface diffusion describes the lateral motion of adsorbed atoms moving between energy minima on the substrate surface. ...
US Navy weapon Surface plasmon resonance of electrons Abbreviation of sapper, a military engineer Strategic Petroleum Reserve ( ...
... and sgk-1 using surface plasmon resonance". Biochim. Biophys. Acta. 1612 (1): 59-64. doi:10.1016/S0005-2736(03)00083-X. PMID ... In mouse, NEDD4-2 deletion leads to increased cell surface expression and activity of ENaC in the lung, resulting in premature ... channel cell surface expression". EMBO J. 20 (24): 7052-9. doi:10.1093/emboj/20.24.7052. PMC 125341. PMID 11742982. Harvey KF, ... "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the ...
World Wireless System
energy portal Wireless energy transmission Surface plasmon Surface-wave-sustained mode Transmission medium Distributed ... He believed that the placement of a grounded resonance transformer at another point on the Earth's surface in the role of a ... The design consisted of a grounded Tesla coil as a resonance transformer transmitter that he thought would be able to create a ... Between 1895 and 1898, he constructed a large resonance transformer in his New York City lab called a magnifying transmitter to ...
... and liquid-gas surfaces. Multi-parametric surface plasmon resonance works in solid-gas, solid-liquid, liquid-gas surfaces and ... surface states, surface diffusion, surface reconstruction, surface phonons and plasmons, epitaxy, the emission and tunneling of ... Jussila, Henri; Yang, He; Granqvist, Niko; Sun, Zhipei (5 February 2016). "Surface plasmon resonance for characterization of ... It includes the fields of surface chemistry and surface physics. Some related practical applications are classed as surface ...
Binding of ligand can be characterised using a variety of analytical techniques such as surface plasmon resonance, microscale ...
At surfaces it is possible for so called image states to occur, where the hole is inside the solid and the electron is in the ... Orbiton Oscillator strength Plasmon Polariton superfluid Trion R. S. Knox, Theory of excitons, Solid state physics (Ed. by ... The recombination of the electron and hole, i.e., the decay of the exciton, is limited by resonance stabilization due to the ... These electron-hole pairs can only move along the surface. Alternatively, an exciton may be described as an excited state of an ...
Index of physics articles (S)
... phonon Surface photovoltage Surface plasmon Surface plasmon polaritons Surface plasmon resonance Surface power density Surface ... fine structure Surface force Surface forces apparatus Surface freezing Surface gravity Surface layer Surface phenomenon Surface ... Surface charge Surface conductivity Surface core level shift Surface energy Surface equivalence principle Surface extended X- ... Feynman! Surface-tension values Surface Evolver Surface Science Reports Surface Water Simulation Modelling Programme Surface X- ...
China Medical Technologies
... and surface plasmon resonance ("SPR") technology to detect and monitor various diseases and disorders. CMED ceased operating in ... and surface plasmon resonance ("SPR") technology to detect and monitor various diseases and disorders. The ECLIA business ...
... a commonly-used experimental setup for the excitation of surface plasmon resonances. From 1944 to 1946 he was a professor of ... Surface Plasmons on Smooth and Rough Surfaces and on Gratings. New York: Springer. ISBN 3540173633. Raether limit Surface ... Raether, H. (July-August 1967). "Surface plasma oscillations as a tool for surface examinations". Surface Science. 8 (1-2): 233 ... He is best known for his theoretical and experimental contributions to the study of surface plasmons, as well as for ...
... using localized surface plasmon resonance, broaden the spectral range of her photocatalytic materials. Ye was elected Fellow of ... In particular, Ye has developed nano-structured surfaces that enhance reactivities, and, ...
Infrared Nanospectroscopy (AFM-IR)
AFM-IR has been used to study the surface plasmon resonance in heavily silicon-doped indium arsenide microparticles. Gold split ... Additionally, the resonance becomes broader for materials with larger dissipation. These contact resonances have been studied ... a coupled resonance resulting from the properties of both the cantilever and the stiffness and damping of the sample surface. ... 15 grammes A contact resonance is a vibrational resonance frequency of an AFM cantilever that occurs when the tip of the AFM is ...
Accurate high-throughput surface plasmon resonance
SF3 Surface Plasmon Resonance (SPR) instrument for high throughput hit identification ... In this video, discover the Octet® SF3 Surface Plasmon Resonance (SPR) instrument for high throughput hit identification. ... Powered and prepared with accurate high-throughput surface plasmon resonance. 4 May 2022 ...
APS -2009 APS March Meeting - Event - A Surface Plasmon Resonance Investigation of How Water Meets a Hydrophobic Surface.
Abstract: L14.00015 : A Surface Plasmon Resonance Investigation of How Water Meets a Hydrophobic Surface.. 5:18 PM-5:30 PM ... We have employed the surface-sensitive, quantum-optical technique of Surface Plasmon Resonance (SPR) to verify the existence of ... Water placed on a hydrophobic surface will form a drop in order to minimize its contact area. What happens when water is forced ... into contact with a hydrophobic surface? One theory is that an ultra-thin low- density region forms near the surface. ...
PRIME PubMed | Labeled gold nanoparticles immobilized at smooth metallic substrates: systematic investigation of surface...
... systematic investigation of surface plasmon resonance and surface-enhanced Raman scattering. Download Prime PubMed App to ... systematic investigation of surface plasmon resonance and surface-enhanced Raman scattering. The Journal of Physical Chemistry ... on the surface plasmon resonance (SPR) of labeled gold nanoparticles and the concomitant impact on the surface-enhanced Raman ... Systematic Investigation of Surface Plasmon Resonance and Surface-enhanced Raman Scattering. J Phys Chem B. 2006 Sep 7;110(35): ...
Surface plasmon resonances in liquid metal nanoparticles - Русский
Surface plasmon resonances in liquid metal nanoparticles. Ershov, A. E.; Gerasimov, V. S.; Gavrilyuk, A. P.; Karpov, S. V. ... Surface plasmon resonances in liquid metal nanoparticles Ershov, A. E.; Gerasimov, V. S.; Gavrilyuk, A. P.; Karpov, S. V. ... for single Au nanoparticles heated to the melting temperature and above up to full suppression of the surface plasmon resonance ... We have shown significant suppression of resonant properties of metallic nanoparticles at the surface plasmon frequency during ...
Absorption as a selective mechanism in surface plasmon resonance fiber optic sensors - E-Prints Complutense
A new concept of surface plasmon resonance fiber optic sensor is presented. By tuning the plasmon resonance to a wavelength for ... Multiple surface plasmon resonance, obtainable in those structures, can be used for the development of microspectrometers based ... Absorption as a selective mechanism in surface plasmon resonance fiber optic sensors ... Absorption as a selective mechanism in surface plasmon resonance fiber optic sensors. Optics Letters, 31 (21). pp. 3089-3091. ...
Surface Plasmon Resonance Laboratory
... in particular the attempt of predicting and generating a resonance condition in a three layer system; glass, metal and air. The ... This lab focuses on the optical generation and modeling of Surface Plasmons, ... E. Sánchez, Surface Plasmon Resonance Laboratory, presented at the AAPT Summer Meeting - Workshop 38: Advanced Labs, Portland, ... "Surface Plasmon Resonance Laboratory." AAPT Summer Meeting - Workshop 38: Advanced Labs. Portland, Oregon: 2010. of American ...
Corrigendum: High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method<...
keywords = "Chemistry, surface plasmon, biosensing and bioimaging, surface plasmon sensors, biophotonics and plasmonics, ... Corrigendum: High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method. In: ... Corrigendum: High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method. / Zeng, ... Corrigendum: High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method. Frontiers ...
Localized surface plasmon resonances after selective oxidization of AuCu solid solution nanocrystalline films - FORTH / ICE-HT
Study of the surface plasmon resonances in silicon nanowires with diameters less than 100 nm</em>...
Borgh G, Bongiorno C, La Magna A, Mannino G, Patanè S, Adam J et al.. Study of the surface plasmon resonances in silicon ... Study of the surface plasmon resonances in silicon nanowires with diameters less than 100 nm. Abstract fra MRS-Materials ... Study of the surface plasmon resonances in silicon nanowires with diameters less than 100 nm. / Borgh, Giovanni; Bongiorno, ... In this talk, the surface plasmon resonances triggered in isolated Si-NWs with diameters below than 100 nm are visualized at ...
Sensors for Continuous Measuring of Sucrose Solutions Using Surface Plasmon Resonance
Statistical Modelling of Photonic Crystal Fibre based Surface Plasmon Resonance Sensors Resonant Peak Wavelength for Tolerance...
Osifeso, S., Chu, S., & Nakkeeran , K. (2021). Statistical Modelling of Photonic Crystal Fibre based Surface Plasmon Resonance ... Osifeso, Samuel ; Chu, Suoda ; Nakkeeran , K. / Statistical Modelling of Photonic Crystal Fibre based Surface Plasmon Resonance ... Osifeso, S, Chu, S & Nakkeeran , K 2021, Statistical Modelling of Photonic Crystal Fibre based Surface Plasmon Resonance ... Dive into the research topics of Statistical Modelling of Photonic Crystal Fibre based Surface Plasmon Resonance Sensors ...
Plasmonic nanostructured substrates for Surface Plasmon Resonance sensors - Archive ouverte HAL
Effective refractive index of calcium carbonate pigment slurries by a surface-plasmon-resonance sensor<...
A surface-plasmon-resonance sensor was applied in the measurement of the effective refractive index of highly turbid calcium ... N2 - A surface-plasmon-resonance sensor was applied in the measurement of the effective refractive index of highly turbid ... AB - A surface-plasmon-resonance sensor was applied in the measurement of the effective refractive index of highly turbid ... abstract = "A surface-plasmon-resonance sensor was applied in the measurement of the effective refractive index of highly ...
Modeling of vapor sorption in polymeric film studied by surface plasmon resonance spectroscopy - Sheffield Hallam University...
Sorption process by surface plasmon resonance (SPR) was studied by exposing polymeric film made from anthracene labeled poly( ... Modeling of vapor sorption in polymeric film studied by surface plasmon resonance spectroscopy ... Modeling of vapor sorption in polymeric film studied by surface plasmon resonance spectroscopy. Journal of colloid and ...
Controlling the SARS-CoV-2 spike glycoprotein conformation | Nature Structural & Molecular Biology
Surface plasmon resonance. The binding of antibody CR3022 and ACE-2 to the SARS-CoV-2 spike constructs was assessed by SPR, in ... was measured by surface plasmon resonance (SPR). Data shown are means and s.d. of three technical repeats and are ... The surface was regenerated between injections by flowing over 3 M MgCl2 solution for 10 s with a flow rate of 100 µl min−1. To ... The surface was regenerated between injections by flowing over SA regeneration buffer (1 M NaCl, 50 mM NaOH) solution for 10 s ...
Development of a combined surface plasmon resonance/surface acoustic wave device for the characterization of biomolecules -...
This work reports on the development of a combined surface plasmon resonance/surface acoustic wave sensor system which is ... Development of a combined surface plasmon resonance/surface acoustic wave device for the characterization of biomolecules ... Development of a combined surface plasmon resonance/surface acoustic wave device for the characterization of biomolecules. ...
Surface Plasmon Resonance Laboratory
... in particular the attempt of predicting and generating a resonance condition in a three layer system; glass, metal and air. The ... This lab focuses on the optical generation and modeling of Surface Plasmons, ... E. Sánchez, Surface Plasmon Resonance Laboratory, presented at the AAPT Summer Meeting - Workshop 38: Advanced Labs, Portland, ... "Surface Plasmon Resonance Laboratory." AAPT Summer Meeting - Workshop 38: Advanced Labs. Portland, Oregon: 2010. of American ...
In situ investigations on electrochemical deposition and properties of polyaniline ultrathin films: Electrochemical- surface...
... surface plasmon resonance spectroscopy using two wavelengths. Abstracts of Papers of the American Chemical Society, 223, 076- ... surface plasmon resonance spectroscopy using two wavelengths. Stefani, F. D., Baba, A., Knoll, W., Xia, C., & Advincula, R. ( ... surface plasmon resonance spectroscopy using two wavelengths. ...
Localized surface plasmon resonance (LSPR) | Nadetech Innovations
... and lossy mode resonance (LMR). Gold nanoparticles (AuNPs), Localized surface plasmon resonance (LSPR), Lossy Mode Resonance ( ... Cladding removed optical fibers, Gold nanorods (GNRs), Localized surface plasmon resonance (LSPR), Lossy Mode Resonance (LMR), ... Dip coating, Glass, Gold nanoparticles (AuNPs), Localized surface plasmon resonance (LSPR), Lossy Mode Resonance (LMR), ... Dip coating, Gold nanoparticles (AuNPs), Localized surface plasmon resonance (LSPR), Lossy Mode Resonance (LMR), Optical fiber ...
Cardiac Actin assays for surface plasmon resonance
The Actin-Toolkit SPR provides a unique method to precisely analyze ligand-F-actin interactions by Surface Plasmon Resonance ... After the actin filaments become attached to the surface of the chip, ligands can be introduced to investigate binding to F- ... The actin filaments protrude from the surface of the chip and permit the introduction of ligands. ...
Identification of PqsD inhibitors using surface plasmon resonance
Local Surface Plasmon Resonance - Globe Thesis - Globe Thesis
Local Surface Plasmon Resonance]. Result: 1 - 7 , Page: 1 of 1. 1. Research On The Use Of Surface Plasmon Propagation Of Light ... Research On The Regulation Characteristics Of Multi-band Surface Plasmon Resonance Sensing Structure. ... Study On Luminescence Characteristics Of Semiconductor Materials Enhanced By Plasma Exciton On Metal Surface. ...
Battery Manufacturing/Recycling - HORIBA
Surface Plasmon Resonance Market Size - Article Zone - Bloggers Unite India
Surface plasmon resonance-mass spectrometry - Patent US-5955729-A - PubChem
Surface Plasmon Resonance Facility | Sir William Dunn School of Pathology
Kondenserade materiens fysik (fasta tillståndets fysik & vätskefysik) - Materietillstånd - Fysik - Matematik & Naturvetenskap -...
Part I introduces principles of surface plasmon resonance bio-sensors, electromagnetic theory of surface plasmons, theory of ... Surface Plasmon Resonance Sensors av Leiva Casemiro Oliveira, Antonio Marcus Nogueira Lima, Carsten Thirstrup, Helmut Franz ... Surface Plasmon Resonance Based Sensors av Jiri Homola Inbunden, Engelska, 2006-08-01 ... This significantly extended second edition addresses the important physical phenomenon of Surface Plasmon Resonance (SPR) or ...
Effect of oxazaborolidines on immobilized fructosyltransferase analyzed by surface plasmon resonance
... system which involves the use of real-time surface plasmon resonance (SPR) technique. The tested oxazaborolidines demonstrated ... system which involves the use of real-time surface plasmon resonance (SPR) technique. The tested oxazaborolidines demonstrated ... Our results show that oxazaborolidines can act as enzymatic inhibitors of FTF immobilized on the surface, also at levels lower ... Our results show that oxazaborolidines can act as enzymatic inhibitors of FTF immobilized on the surface, also at levels lower ...
All-optical switching of localized surface plasmon resonance in single gold nanosandwich using GeSbTe film as an active medium<...
Localized surface plasmon resonance (LSPR) switching was investigated in a Au/GeSbTe/Au nanosandwich as a key active element ... N2 - Localized surface plasmon resonance (LSPR) switching was investigated in a Au/GeSbTe/Au nanosandwich as a key active ... AB - Localized surface plasmon resonance (LSPR) switching was investigated in a Au/GeSbTe/Au nanosandwich as a key active ... abstract = "Localized surface plasmon resonance (LSPR) switching was investigated in a Au/GeSbTe/Au nanosandwich as a key ...
Refractive indexSpectroscopyLSPRSensitivityPlasmonicWavelengthBiacore T100Sensors basedSensorDetectionAbstractBiosensorNanoparticlesSelective2022BiosensorsElectrochemicalMetrologyMeasurementsPhotonicsCharacterizationInteractionsElectromagneticHomolaFree electronsKineticMeasurementOpticalEnhancementInvestigationSignalsLigandsImagingBindsReflectionExperimentProbeEnergy densityTheoryContaminationFormationModeHighConcentrationAnalysis
- A surface-plasmon-resonance sensor was applied in the measurement of the effective refractive index of highly turbid calcium carbonate pigment slurries. (vtt.fi)
- In particular, the antisymmetric (magnetic resonance) mode can be modulated effectively by the extinction coefficient of GST, as well as its refractive index. (elsevier.com)
- Being highlighted label-free surface plasmon resonance (SPR) optical sensor uses modulation of the light output spectrum caused by locally modulated refractive index (localized surface plasmon resonance (LSPR)) [ 1 - 3 ]. (jecst.org)
- After normalization, plasmonic responses from different particles become highly consistent, creating well-fitted dose−response curves of both surrounding refractive index changes and receptor−analyte binding to the surface of individual nanoparticles. (figshare.com)
- The refractive index of light reflecting off this surface is measured. (bvsalud.org)
- This is a technique with which we can measure the small variation finding in the refractive index near the surface of metal with higher sensitivity. (adpublications.org)
- We characterize the systems through transmission electron microscopy (TEM) coupled to electron en- ergy loss spectroscopy (EELS) with a subnanometer electron probe. (sdu.dk)
- In situ investigations on electrochemical deposition and properties of polyaniline ultrathin films: Electrochemical- surface plasmon resonance spectroscopy using two wavelengths. (mpg.de)
- J.-F. Li, C.-Y. Li and R. F. Aroca, Plasmon-enhanced fluorescence spectroscopy, Chem. (smf.mx)
- Here, this approach is extended by using localized surface plasmon resonance spectroscopy. (insplorion.com)
- In this regard, we comprehensively review the current state-of-the-art ethylene gas sensors and detecting technologies, covering from preconcentrator-equipped gas chromatographic systems, Fourier transform infrared technology, photonic crystal fiber-enhanced Raman spectroscopy, surface acoustic wave and photoacoustic sensors, printable optically colorimetric sensor arrays to a wide range of nanostructured chemiresistive gas sensors (including the potentiometric and amperometric-type FET-, CNT- and metal oxide-based sensors). (mdpi.com)
- Biophysical methods such as X-ray crystallography, NMR spectroscopy, surface plasmon resonance (SPR) spectroscopy, analytical ultra-centrifugation (AUC) and isothermal titration calorimetry (ITC) are being used to study the molecular and structural basis of macromolecular functions. (iitkgp.ac.in)
- Surface-enhanced Raman spectroscopy performed using the functionalized Au stars showed a significant enhancement in Raman signals for a wider range of urea concentration (2-20 mg/ml). (archives-ouvertes.fr)
- Localized surface plasmon resonance (LSPR) switching was investigated in a Au/GeSbTe/Au nanosandwich as a key active element for plasmonic integrated circuits and devices. (elsevier.com)
- An optical detection platform to track down localized surface plasmon resonance (LSPR) signals of individual nanoparticles on substrates was built for the application of plasmonic nanoarrays. (figshare.com)
- Bismuth selenide (Bi 2 Se 3 ) is an orderly layered material with large surface area and localized surface plasmon resonance (LSPR). (uai.cl)
- Based on Localized Surface Plasmon Resonance (LSPR) of these core-shells NPs, the prepared array detected CO gas flow at the level of one Liter per hour at room temperature. (ac.ir)
- The physical absorption of CO gas on the surface of [email protected] core-shell NPs increases the LSPR absorbance and causes red-shift of LSPR peak. (ac.ir)
- The project is based on the sensitivity of surface plasmons to the voltage at the metal-electrolyte interface. (nottingham.ac.uk)
- In order to study the voltage sensitivity of surface plasmon resonance, an SPR-basedvoltage sensing system has been designed and tested. (nottingham.ac.uk)
- We propose an idea for improving the angular sensitivity of Kretschmann-type surface plasmon resonance (SPR) sensors through the use of highrefractive- index silver nanoparticle (AgNP) sheets on metal substrates. (elsevier.com)
- G. Xia, C. Zhou, S. Jin, C. Huang, J. Xing and Z. Liu, Sensitivity Enhancement of Two-Dimensional Materials Based on Genetic Optimization in Surface Plasmon Resonance, Sensors (Basel) 19 (2019) 1198, https://doi.org/10.3390/s19051198 . (smf.mx)
- Abstract - In recent years Surface Plasmon Resonance (SPR) is gaining large interest in research areas because of its fast response and its high sensitivity. (adpublications.org)
- The fabricated sub-wavelength structures, tailored in size and geometry, possess strong, localized, plasmonic resonances able to control linear polarization. (semanticscholar.org)
- In this paper, we propose a design process for a plasmonic grating structure to induce the resonance at a target wavelength. (elsevier.com)
- The surface and interfacial engineering strategy proposed here can be extended to tune plasmonic resonance and charge carrier energy density for the successful implementation of Bi 2 Se 3 beyond energy storage applications. (uai.cl)
- By tuning the plasmon resonance to a wavelength for which the outer medium is absorptive, a significant variation of the spectral transmittance of the device is produced as a function of the concentration of the analyte. (ucm.es)
- We report a statistical approach to model the resonant peak wavelength (RPW) equation(s) of a photonic crystal fibre (PCF)-based surface plasmon resonance (SPR) sensors in terms of the PCF structural parameters (air-hole diameter, pitch, core diameter and gold layer thickness) at various tolerance levels. (elsevier.com)
- Due to the narrow bandwidth of the wavelength consumed by the excitation of the surface plasmons, a high FOM with a narrow full width half maximum (FWHM, nm) in the optical spectrum allows the resonance to be observed more clearly through an effective and accurate sensing process. (jecst.org)
- In a specific condition, surface gratings may induce the surface plasmon propagation due to the diffraction and the resonance wavelength of the surface plasmon propagation is dependent on the grating shape. (elsevier.com)
- To shift the resonance wavelength to the target wavelength, this paper maps the frequency response function to a probability distribution function (PDF). (elsevier.com)
- The mean value from the PDF becomes a part of the objective function and the design objective is set to minimize the distance between the present resonance wavelength and the target wavelength while keeping the energy intensity. (elsevier.com)
- Seong, HK & Yoo, J 2018, ' Systematic design of a grating structure to induce the surface plasmon resonance at a target wavelength ', IEEE Transactions on Magnetics , vol. 54, no. 3, 8094891. (elsevier.com)
- Z. Chen, L. Liu, Y. He and H. Ma, Resolution enhancement of surface plasmon resonance sensors with spectral interrogation: resonant wavelength considerations, Appl. (smf.mx)
- Our Surface Plasmon Resonance (SPR) Biacore T100 biosensor instrument is a high-throughput equipment that allows the study of protein-protein and protein-ligand kinetics and thermodynamics. (utmb.edu)
- We propose two new sensors based on surface plasmon resonance (SPR) and optical fibers to determine the concentration of sucrose in products such as beverages, honey, condensed milk, etc., in real-time during the fabrication process or when the product has been manufactured. (ugr.es)
- A. K. Sharma, R. Jha and B. D. Gupta, Fiber-Optic Sensors Based on Surface Plasmon Resonance: A Comprehensive Review, IEEE Sensors Journal 7 (2007) 1118, https://doi.org/10.1109/JSEN.2007.897946 . (smf.mx)
- A new concept of surface plasmon resonance fiber optic sensor is presented. (ucm.es)
- With this mechanism, selectivity can be achieved without the need of any functionalization of the surfaces or the use of recognizing elements, which is a very interesting feature for any kind of chemical sensor or biosensor. (ucm.es)
- This work reports on the development of a combined surface plasmon resonance/surface acoustic wave sensor system which is designed for the investigation of biomolecules such as proteins or DNA. (northumbria.ac.uk)
- Herein, the hybridization of the target and the probe DNA on the sensor surface and its dependence on the surface probe DNA coverage and the ionic strength were systematically investigated by surface plasmon resonance (SPR). (scilifelab.se)
- We found that under physiological solutions (200 to 300 mM ionic strength), the ISFET sensor could not register the DNA hybridization events on the sensor surface due to Debye screening. (scilifelab.se)
- Based on the characterization of the stability of the hybridized DNA duplexes on the sensor surface in low salt concentration buffer solutions, the estimated maximum potentiometric signal could be significantly higher using the alternative method. (scilifelab.se)
- As a novel optical sensor, surface plasmon resonance (SPR) sensors have shown diverse applications in the field of disease diagnosis. (sioc-journal.cn)
- Estimating Detection Limits of Potentiometric DNA Sensors Using Surface Plasmon Resonance Analyses. (scilifelab.se)
- With the method of diluting buffer, the maximum potentiometric signal generated by the DNA hybridization was estimated to be only around 120 mV with the lowest detection limit of 30 nM, occurring on a surface with optimized probe coverage and in the tris buffer with 10 mM NaCl. (scilifelab.se)
- This project was directed at researching the engineering fundamentals of label-free detection of bio-electrical signals using surface plasmon resonance (SPR). (nottingham.ac.uk)
- J. Homola, Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species, Chem. (smf.mx)
- R. Singh, P. Thakur, A. Thakur, H. Kumar, P. Chawla, J. V. Rohit, R. Kaushik and N. Kumar, Colorimetric sensing approaches of surface-modified gold and silver nanoparticles for detection of residual pesticides: a review, International Journal of Environmental Analytical Chemistry 101 (2021) 3006, https://doi.org/10.1080/03067319.2020.1715382 . (smf.mx)
- 1. Tawil N, Mouawad F, Lévesque S, Sacher E, Mandeville R, Meunier M. The differential detection of methicillin-resistant, methicillin-susceptible and borderline oxacillin-resistant Staphylococcus aureus by surface plasmon resonance. (cdc.gov)
- FRANCISCO J. ARREGUI Abstract An optical fiber device showing simultaneously two optical phenomena, localized surface plasmon resonance. (nadetech.com)
- CiBIT is equipped with unique in Greece state-of-the-art equipment, a customized multi-laser line modular confocal microscope and a multiplex surface plasmon resonance (SPR) biosensor. (europa.eu)
- This paper experimentally and theoretically investigates the influence of an underlying metallic substrate (i.e., gold and silver) on the surface plasmon resonance (SPR) of labeled gold nanoparticles and the concomitant impact on the surface-enhanced Raman scattering (SERS) signal from the labels. (unboundmedicine.com)
- These experiments employ nanoparticles of varied sizes (30-100 nm) that are coated with a bifunctional Raman scatterer composed of (1) a disulfide for chemisorption to the nanoparticle surface, (2) a succinimidyl ester for formation of a covalent linkage to an amine-terminated self-assembled monolayer on the underlying substrate, and (3) an aryl nitro group with an intrinsically strong Raman active vibrational mode. (unboundmedicine.com)
- We have shown significant suppression of resonant properties of metallic nanoparticles at the surface plasmon frequency during the phase transition "solid-liquid" in the basic materials of nanoplasmonics (Ag, Au). (kirensky.ru)
- Experimental verification was performed for single Au nanoparticles heated to the melting temperature and above up to full suppression of the surface plasmon resonance. (kirensky.ru)
- Most importantly, we present a method to normalize the localized surface plasmon resonance from geometrically different nanoparticles. (figshare.com)
- Hence, an overview of gas sensors is presented, including electronic-nose systems, followed by a description of the surface plasmons that arise in noble metal thin films and nanoparticles. (academic-accelerator.com)
- Scanning electron microscopy analysis of surfaces produced via the selective laser melting technique by microsecond laser pulses of AlSi10Mg powders in vacuum and argon has been carried out. (semanticscholar.org)
- 2022). Droplet transportation by adjusting the temporal phase shift of surface acoustic waves in the exciter-exciter mode . (city.ac.uk)
- As a result, nanostructure-based grating couplers can successfully expand the practicality of plasmon sensors in a wide range of biosensors, photocatalytic, and heterogeneous catalytic researches [ 6 - 8 ]. (jecst.org)
- M. Piliarik, H. Vaisocherov and J. Homola, Surface Plasmon Resonance Biosensing, Biosensors and Biodetection 503 (2009) 65, https://doi.org/10.1007/978-1-60327-567-5 5. (smf.mx)
- In particular, surface relaxation or reconstruction is usually observed through thermal heat-treatment (or annealing, firing) or electrochemical treatment [ 11 ]. (jecst.org)
- Optical Metrology, Precise Positioning, Surface Plasmon Resonance Sensing. (ncu.edu.tw)
- Measurements of surface plasmon resonance indicate that a monomer of T7 DNA polymerase binds to a dimer of gp4, gp4-C219, or gp4-C241 but to a monomer of gp4-C272. (nih.gov)
- Focus Issue on surface plasmon photonics introduction. (semanticscholar.org)
- The 7th International Conference on Surface Plasmon Photonics (SPP7) was held in Jerusalem, Israel from May 31st to June 5th, 2015. (semanticscholar.org)
- A. A. Kolomenskii, P. D. Gershon and H. A. Schuessler, Surface-plasmon resonance spectrometry and characterization of absorbing liquids, Appl. (smf.mx)
- The Actin-Toolkit SPR provides a unique method to precisely analyze ligand-F-actin interactions by Surface Plasmon Resonance techniques on Streptavidin chips. (hypermol.com)
- Surface Plasmon Resonance (SPR) is a widely used and extremely powerful technique for studying molecular interactions. (ox.ac.uk)
- Part I introduces principles of surface plasmon resonance bio-sensors, electromagnetic theory of surface plasmons, theory of SPR sensors and molecular interactions at s. (bokus.com)
- We screened over 20,000 compounds with Npas4 through small-molecule microarrays (SMMs) and confirmed interactions with surface plasmon resonance (SPR). (broadinstitute.org)
- We study light-matter interactions leading to the generation of photon drag voltage under surface plasmon resonance conditions in noble metal thin films and observe important effects, which provide opportunity for condensed matter theorists to critically evaluate theoretical models. (academic-accelerator.com)
- This is because they show interesting optical properties, including plasmon resonance (PR), the collective oscillation of free electrons, induced by an electromagnetic field at the proper frequency. (sdu.dk)
- Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. (bvsalud.org)
- The SensiQ technology uses advanced microfluidics, proven surface attachment methodologies and state-of-the-art data analysis tools to provide kinetic, affinity and concentration data that researchers can use with confidence. (spectraresearch.com)
- We have employed the surface-sensitive, quantum-optical technique of Surface Plasmon Resonance (SPR) to verify the existence of this region at the boundary. (aps.org)
- Generation and monitoring of optical resonances. (nadetech.com)
- The optical con�guration is used toexcite surface plasmons at the metal-electrolyte interface while the electrochemicalunit is used to control the potential at this interface. (nottingham.ac.uk)
- The gold star dispersions display a well-defined optical response as observed by the presence of a sharp surface plasmon resonance centered at 525 nm. (archives-ouvertes.fr)
- These results are then correlated with SERS enhancement factors (EFs) that were determined by accounting for particle surface concentrations, which were measured by atomic force microscopy, and the absolute number of labels, which were calculated on the basis of the surface area of each of the different-sized particles. (unboundmedicine.com)
- A Surface Plasmon Resonance Investigation of How Water Meets a Hydrophobic Surface. (aps.org)
- Surface plasmon resonance (SPR) has emerged as a popular technique for elucidating subtle signals from biological events in a label-free, high throughput environment. (asu.edu)
- The actin filaments protrude from the surface of the chip and permit the introduction of ligands. (hypermol.com)
- After the actin filaments become attached to the surface of the chip, ligands can be introduced to investigate binding to F-actin. (hypermol.com)
- When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. (bvsalud.org)
- Microfluidic device for immunoassays based on surface plasmon resonance imaging. (bvsalud.org)
- We use surface plasmon resonance (SPR) imaging to monitor the antibody- antigen recognition and binding events. (bvsalud.org)
- Surface plasmon resonance analysis revealed that NS5A binds to the Fyn SH3 domain with what can be considered a high affinity SH3 domain-ligand interaction (629 nM), and this binding did not require the presence of domain I of NS5A (amino acid residues 32-250). (biomedcentral.com)
- The Au stars functionalized with 11-mercaptoundecanoic acid and immobilized with urease showed improved urea-sensing behavior in comparison to pure gold disk in attenuated total reflection surface plasmon resonance (ATR SPR) mode. (archives-ouvertes.fr)
- Recently, scientists at the Helmholtz Institute for Pharmaceutical Research Saarland in Germany conducted an experiment using surface plasmon resonance (SPR) to characterize the binding properties of compounds in this communication system. (reichertspr.com)
- This adverse effect of low salt concentration on the hybridization efficiency was also found to be more significant on the surface with higher probe coverage due to steric hindrance. (scilifelab.se)
- Finally, it causes accumulative energy density at a surface by smoothing effect and redistribution by missing neighbor. (jecst.org)
- One theory is that an ultra-thin low- density region forms near the surface. (aps.org)
- Field methods are needed to assess the contamination of surfaces by methamphetamine from illicit drug manu facturing. (cdc.gov)
- This study performed a feasibility study on the use of a surface plasmon resonance (SPR) based instrument (SensiQ Discovery) in the evaluation of surface contamination by methamphetamine. (cdc.gov)
- Methamphetamine was spiked onto ceramic tiles and the assay was able to detect methamphet amine contamination at 25 ng/100 cm(2), which is below the 50 ng/100 cm(2) standard used for surface cleanup assessment. (cdc.gov)
- Background: Methicillin-resistant Staphylococcus aureus contamination on surfaces including turnout gear had been found throughout a number of fire stations. (cdc.gov)
- The second is reconstruction in which the periodicity of the unit cell and the surface changes, and the third is the formation of a surface oxide layer by ad-atoms or ad-molecules. (jecst.org)
- Plasmon resonance data provided by Innexus Biotechnology (AZ, USA) reveal some insight into the mechanism of dimer and lattice formation of homophilic antibodies interacting with their target (Figure 7) [Kindt T, Unpublished Data]. (medscape.com)
- Dertil indføres en såkaldt mode converter imellem den smalle bølgeleder og den bredere, korrugerede bølgeleder, hvis formål det er på effektiv vis at konvertere energien fra den smalle til den korrugerede bølgeleder. (ing.dk)
- In this video, discover the Octet ® SF3 Surface Plasmon Resonance (SPR) instrument for high throughput hit identification. (selectscience.net)
- In this talk, the surface plasmon resonances triggered in isolated Si-NWs with diameters below than 100 nm are visualized at high spatial resolution. (sdu.dk)
- Lowering the salt concentration to enlarge the Debye length would at the same time reduce the surface hybridization efficiency, thus suppressing the signal. (scilifelab.se)
- A. A. Alwahib, Sura H., Al-Rekabi and W. H. Muttlak, Comprehensive study of generating sharp dip using numerical analysis in prism based surface plasmon resonance, AIP Conference Proceedings 2213 (2020) https://doi.org/10.1063/5.0000103 . (smf.mx)