A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques.
Proteins that are chemically bound to a substrate material which renders their location fixed. The immobilization of proteins allows their use in chemical reactions without being diluted by solvent.
The rate dynamics in chemical or physical systems.
Measurement of the index of refraction (the ratio of the velocity of light or other radiation in the first of two media to its velocity in the second as it passes from one into the other).
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Proteins prepared by recombinant DNA technology.
Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
Thin strands of transparent material, usually glass, that are used for transmitting light waves over long distances.
Characteristics or attributes of the outer boundaries of objects, including molecules.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
Incorporation of biotinyl groups into molecules.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
Antibodies produced by a single clone of cells.
Proteins found in any species of bacterium.
Antibodies that are chemically bound to a substrate material which renders their location fixed.
Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Nanometer-sized tubes composed of various substances including carbon (CARBON NANOTUBES), boron nitride, or nickel vanadate.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
The use of a quartz crystal microbalance for measuring weights and forces in the micro- to nanogram range. It is used to study the chemical and mechanical properties of thin layers, such as polymer coatings and lipid membranes; and interactions between molecues.
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A methodology for chemically synthesizing polymer molds of specific molecules or recognition sites of specific molecules. Applications for molecularly imprinted polymers (MIPs) include separations, assays and biosensors, and catalysis.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN SUBUNITS.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Established cell cultures that have the potential to propagate indefinitely.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
A genus of plant viruses in the family CLOSTEROVIRIDAE containing highly flexuous filaments. Some members are important pathogens of crop plants. Natural vectors include APHIDS, whiteflies, and mealybugs. The type species is Beet yellows virus.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Methods of creating machines and devices.
An anticoccidial agent mainly for poultry.
Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Elements of limited time intervals, contributing to particular results or situations.
LIGHT, it's processes and properties, and the characteristics of materials interacting with it.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Transport proteins that carry specific substances in the blood or across cell membranes.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Methods for determining interaction between PROTEINS.
The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).
Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.
Protein modules with conserved ligand-binding surfaces which mediate specific interaction functions in SIGNAL TRANSDUCTION PATHWAYS and the specific BINDING SITES of their cognate protein LIGANDS.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Sites on an antigen that interact with specific antibodies.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Works about studies performed to evaluate the safety of diagnostic, therapeutic, or prophylactic drugs, devices, or techniques in healthy subjects and to determine the safe dosage range (if appropriate). These tests also are used to determine pharmacologic and pharmacokinetic properties (toxicity, metabolism, absorption, elimination, and preferred route of administration). They involve a small number of persons and usually last about 1 year. This concept includes phase I studies conducted both in the U.S. and in other countries.
A low affinity interleukin-5 receptor subunit that combines with the CYTOKINE RECEPTOR COMMON BETA SUBUNIT to form a high affinity receptor for INTERLEUKIN-5. Several isoforms of the interleukin-5 receptor alpha subunit exist due to multiple ALTERNATIVE SPLICING.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
Measurement of the intensity and quality of fluorescence.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
Behavior of LIGHT and its interactions with itself and materials.

The role of homophilic binding in anti-tumor antibody R24 recognition of molecular surfaces. Demonstration of an intermolecular beta-sheet interaction between vh domains. (1/3140)

The murine antibody R24 and mouse-human Fv-IgG1(kappa) chimeric antibody chR24 are specific for the cell-surface tumor antigen disialoganglioside GD3. X-ray diffraction and surface plasmon resonance experiments have been employed to study the mechanism of "homophilic binding," in which molecules of R24 recognize and bind to other molecules of R24 though their heavy chain variable domains. R24 exhibits strong binding to liposomes containing disialoganglioside GD3; however, the kinetics are unusual in that saturation of binding is not observed. The binding of chR24 to GD3-bearing liposomes is significantly weaker, suggesting that cooperative interactions involving antibody constant regions contribute to R24 binding of membrane-bound GD3. The crystal structures of the Fabs from R24 and chR24 reveal the mechanism for homophilic binding and confirm that the homophilic and antigen-binding idiotopes are distinct. The homophilic binding idiotope is formed largely by an anti-parallel beta-sheet dimerization between the H2 complementarity determining region (CDR) loops of two Fabs, while the antigen-binding idiotope is a pocket formed by the three CDR loops on the heavy chain. The formation of homophilic dimers requires the presence of a canonical conformation for the H2 CDR in conjunction with participation of side chains. The relative positions of the homophilic and antigen-binding sites allows for a lattice of GD3-specific antibodies to be constructed, which is stabilized by the presence of the cell membrane. This model provides for the selective recognition by R24 of cells that overexpress GD3 on the cell surface.  (+info)

A soluble form of the avian hepatitis B virus receptor. Biochemical characterization and functional analysis of the receptor ligand complex. (2/3140)

Avian hepatitis B virus infection is initiated by the specific interaction of the extracellular preS part of the large viral envelope protein with carboxypeptidase D (gp180), the primary cellular receptor. To functionally and biochemically characterize this interaction, we purified a soluble form of duck carboxypeptidase D from a baculovirus expression system, confirmed its receptor function, and investigated the contribution of different preS sequence elements to receptor binding by surface plasmon resonance analysis. We found that preS binds duck carboxypeptidase D with a 1:1 stoichiometry, thereby inducing conformational changes but not oligomerization. The association constant of the complex was determined to be 2.2 x 10(7) M-1 at 37 degreesC, pH 7.4, with an association rate of 4.0 x 10(4) M-1 s-1 and a dissociation rate of 1.9 x 10(-3) s-1, substantiating high affinity interaction of avihepadnaviruses with their receptor carboxypeptidase D. The separately expressed receptor-binding domain, comprising about 50% of preS as defined by mutational analysis, exhibits similar constants. The domain consists of an essential element, probably responsible for the initial receptor contact and a part that contributes to complex stabilization in a conformation sensitive manner. Together with previous results from cell biological studies these data provide new insights into the initial step of hepadnaviral infection.  (+info)

The L1 major capsid protein of human papillomavirus type 11 recombinant virus-like particles interacts with heparin and cell-surface glycosaminoglycans on human keratinocytes. (3/3140)

The L1 major capsid protein of human papillomavirus (HPV) type 11, a 55-kDa polypeptide, forms particulate structures resembling native virus with an average particle diameter of 50-60 nm when expressed in the yeast Saccharomyces cerevisiae. We show in this report that these virus-like particles (VLPs) interact with heparin and with cell-surface glycosaminoglycans (GAGs) resembling heparin on keratinocytes and Chinese hamster ovary cells. The binding of VLPs to heparin is shown to exhibit an affinity comparable to that of other identified heparin-binding proteins. Immobilized heparin chromatography and surface plasmon resonance were used to show that this interaction can be specifically inhibited by free heparin and dextran sulfate and that the effectiveness of the inhibitor is related to its molecular weight and charge density. Sequence comparison of nine human L1 types revealed a conserved region of the carboxyl terminus containing clustered basic amino acids that bear resemblance to proposed heparin-binding motifs in unrelated proteins. Specific enzymatic cleavage of this region eliminated binding to both immobilized heparin and human keratinocyte (HaCaT) cells. Removal of heparan sulfate GAGs on keratinocytes by treatment with heparinase or heparitinase resulted in an 80-90% reduction of VLP binding, whereas treatment of cells with laminin, a substrate for alpha6 integrin receptors, provided minimal inhibition. Cells treated with chlorate or substituted beta-D-xylosides, resulting in undersulfation or secretion of GAG chains, also showed a reduced affinity for VLPs. Similarly, binding of VLPs to a Chinese hamster ovary cell mutant deficient in GAG synthesis was shown to be only 10% that observed for wild type cells. This report establishes for the first time that the carboxyl-terminal portion of HPV L1 interacts with heparin, and that this region appears to be crucial for interaction with the cell surface.  (+info)

Cluster of differentiation antigen 4 (CD4) endocytosis and adaptor complex binding require activation of the CD4 endocytosis signal by serine phosphorylation. (4/3140)

Cluster of differentiation antigen 4 (CD4), the T lymphocyte antigen receptor component and human immunodeficiency virus coreceptor, is down-modulated when cells are activated by antigen or phorbol esters. During down-modulation CD4 dissociates from p56(lck), undergoes endocytosis through clathrin-coated pits, and is then sorted in early endosomes to late endocytic organelles where it is degraded. Previous studies have suggested that phosphorylation and a dileucine sequence are required for down-modulation. Using transfected HeLa cells, in which CD4 endocytosis can be studied in the absence of p56(lck), we show that the dileucine sequence in the cytoplasmic domain is essential for clathrin-mediated CD4 endocytosis. However, this sequence is only functional as an endocytosis signal when neighboring serine residues are phosphorylated. Phosphoserine is required for rapid endocytosis because CD4 molecules in which the cytoplasmic domain serine residues are substituted with glutamic acid residues are not internalized efficiently. Using surface plasmon resonance, we show that CD4 peptides containing the dileucine sequence bind weakly to clathrin adaptor protein complexes 2 and 1. The affinity of this interaction is increased 350- to 700-fold when the peptides also contain phosphoserine residues.  (+info)

Purified meningococcal transferrin-binding protein B interacts with a secondary, strain-specific, binding site in the N-terminal lobe of human transferrin. (5/3140)

Neisseria meningitidis, grown in iron-limited conditions, produces two transferrin-binding proteins (TbpA and TbpB) that independently and specifically bind human serum transferrin (hTF) but not bovine serum transferrin (bTF). We have used surface plasmon resonance to characterize the interaction between individual TbpA and TbpB and a series of full-length human-bovine chimaeric transferrins (hbTFs) under conditions of variable saturation with iron. A comparative analysis of hTF and hbTF chimaera-binding data confirmed that the major features involved in Tbp binding are located in the C-terminal lobe of hTF and that isolated TbpA can recognize distinct sites present in, or conformationally influenced by, residues 598-679. Binding by TbpB was maintained at a significant but decreased level after replacement of the entire hTF C-terminal lobe by the equivalent bovine sequence. The extent of this binding difference was dependent on the meningococcal strain and on the presence of hTF residues 255-350. This indicated that TbpB from strain SD has a secondary, strain-specific, binding site located within this region, whereas TbpB from strain B16B6 does not share this recognition site. Binding of TbpA was influenced primarily by sequence substitutions in the hTF C-terminal lobe, and co-purified TbpA and TbpB (TbpA+B) was functionally distinct from either of its components. The limited divergence between hTF and bTF has been related to observed differences in binding by Tbps and has been used to delineate those regions of hTF that are important for such interactions.  (+info)

Kinetics of the interaction of endotoxin with polymyxin B and its analogs: a surface plasmon resonance analysis. (6/3140)

Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful side effects. Many peptide-based drugs mimicking the activity of PMB have been synthesized in an attempt to reduce toxicity while still retaining the anti-endotoxic activity. The study attempts to use the recent technique of surface plasmon resonance (SPR), in determining the kinetics of association and dissociation involved in the interaction of endotoxin with a few selected peptides that have structural features resembling PMB. The results, in conjunction with the thermodynamic data derived using isothermal titration calorimetry (ITC), stress the vital role played by amphiphilicity of the peptides and hydrophobic forces in this biologically important interaction.  (+info)

Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptides. (7/3140)

A fluorescein-labeled synthetic peptide, PTTTPITTTTK, was converted into O-glycosylated glycopeptides with various numbers of attached N-acetyl-D-galactosamines (GalNAcs) by in vitro glycosylation with UDP-GalNAc and a microsomal fraction of LS174T human colon carcinoma cells. Glycopeptides with 1, 3, 5, and 6 GalNAc residues (G1, G3, G5, and G6) were obtained, and their sizes were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Their sequences were determined by a peptide sequencer to be PTTTGalNAcPITTTTK for G1, PTGalNAcTTPITGalNAcTGalNAcTTK for G3, PTTGalNAcTGalNAcPITGalNAcTGalNAcTGalNAcTK for G5, and PTGalNAcTGalNAcTGalNAcPITGalNAcTGalNAcTGalNAcTK for G6. A calcium-type human macrophage lectin (HML) was prepared in a recombinant form, and its interaction with these glycopeptides was investigated by surface plasmon resonance (SPR) spectroscopy and fluorescence polarization. The affinity of recombinant HML (rHML) for immobilized glycopeptides increased, as revealed by SPR, in parallel with the number of GalNAc. The highest affinity was obtained when the G6-peptide was immobilized at high density. Fluorescence polarization equilibrium-binding assays also revealed that the affinity of rHML for soluble gly-copeptides increased, depending on the number of attached GalNAcs. Carbohydrate recognition domain (CRD) fragments of HML were prepared, and their affinity for these four glycopeptides was also determined, this affinity was apparently lower than that of rHML. Affinity constants of rHML for the G3- and G5-peptides were 11- and 38-fold higher, respectively, than for the G1-peptide, whereas those of CRD fragments were only 2- and 6-fold higher, respectively. A chemical cross-linking study revealed that rHML but not recombinant CRD forms trimers in an aqueous solution. Thus, preferential binding of densely glycosylated O-linked glycopeptides should be due to the trimer formation of rHML.  (+info)

Effects of microcystins on phosphorylase-a binding to phosphatase-2A: kinetic analysis by surface plasmon resonance biosensor. (8/3140)

Cyclic heptapeptide microcystins are a group of hepatoxicants which exert the cytotoxic effects by inhibiting the catalytic activities of phosphatase-2A (PP-2A) and phosphatase-1 (PP-1) and thus disrupt the normal signal transduction pathways. Microcystins interact with PP-2A and PP-1 by a two-step mechanism involving rapid binding and inactivation of protein phosphatase catalytic subunit, followed by a slower covalent interaction. It was proposed that inactivation of PP-2A/PP-1 catalytic activity by microcystins precedes covalent adduct formation. In this study, we used a biosensor based on surface plasmon resonance (SPR) to examine the effects of three microcystins, MCLR, MCRR and MCYR, on the binding between PP-2A and its substrate, phosphorylase-a (PL-a), during the first step of the interaction. The SPR biosensor provides real-time information on the association and dissociation kinetics of PL-a with immobilized PP-2A in the absence and presence of microcystins. It was found that the affinity of PL-a to microcystin-bound PP-2A was four times smaller compared to unbound PP-2A, due to 50% decreases in the association rates and two-fold increases in dissociation rates of PL-a binding to PP-2A. The results suggest that the rapid binding of microcystins to the PP-2A catalytic site leads to the formation of a noncovalent microcystin/PP-2A adduct. While the adduct formation fully inhibits the catalytic activity of PP-2A, it only results in partial inhibition of the substrate binding. The similar effects of the three microcystins on PP-2A suggest that the toxins bind to PP-2A at the same site and cause similar conformational changes. The present work also demonstrates the potential application of biosensor technology in environmental toxicological research.  (+info)

We report a simple label-free localized surface plasmon resonance sensor that uses the multiple resonances of a U-shaped gold nanostructure to differentiat
In this study, a rationally-designed 2,4,6-trinitrotoluene (TNT) binding peptide derived from an amino acid sequence of the complementarity-determining region (CDR) of an anti-TNT monoclonal antibody was used for TNT detection based on a maleimide-functionalized surface plasmon resonance (SPR) sensor. By antigen-docking simulation and screening, the TNT binding candidate peptides were obtained as TNTHCDR1 derived from the heavy chain of CDR1, TNTHCDR2 derived from CDR2, and TNTHCDR3 from CDR3 of an anti-TNT antibody. The binding events between candidate peptides and TNT were evaluated using the SPR sensor by direct determination based on the 3-aminopropyltriethoxysilane (APTES) surface. The TNT binding peptide was directly immobilized on the maleimide-functionalized sensor chip surface from N-γ-maleimidobutyryl-oxysuccinimide ester (GMBS). The results demonstrated that peptide TNTHCDR3 was identified and selected as a TNT binding peptide among the other two candidate peptides. Five kinds of TNT
We have rationally designed two-dimensional Au and Ag hole arrays for high performing surface plasmon resonance (SPR) sensing. The figure-of-merit (FOM), which is defined as sensitivity/linewidth, is found to be highly geometry-dependent. For sensitivity, we find it is equal to the period of array when exciting low order surface plasmon modes at low incident angle. Therefore, increasing period improves sensitivity. On the other hand, narrow linewidth can be obtained from small hole size so that the radiative decay loss is minimized. By using a pair of orthogonally oriented polarizer and analyzer, the signal-to-noise ratio (SNR) can be greatly enhanced due to the elimination of the nonresonant reflection background. As a proof of our strategy, we have obtained FOM larger than 100/RIU and SNR higher than 110 from Au arrays. Our results show the importance of understanding the basic properties of surface plasmon polaritons in order to systematically optimize the performance of the plasmonic system ...
BioAssay record AID 642885 submitted by ChEMBL: Binding affinity to chicken riboflavin binding protein by surface plasmon resonance assay.
The surface plasmon resonance (SPR) phenomenon is utilized in a number of new real time biosensors. In this study, we have used this technique to study interactions between the central complement component C3b and its multiple ligands by using the Biacore equipment. The SPR technique is particularly suitable for analysis of the alternative complement pathway (AP) because the inherent nature of the latter is to amplify deposition of C3b on various surfaces. C3b was coupled onto the sensor surface and the coupling efficiency was compared under various conditions on both polystyrene and carboxymethylated dextran surfaces. After enzymatic C3b coupling or standard amine C3b coupling, we analyzed and compared the binding of four C3b ligands to the surface: factor B, factor H, C5 and the soluble complement receptor 1 (sCR1, CD35). Binding of each ligand to C3b was detected when C3b had been coupled either enzymatically or using the amine coupling, but the half-lives of the interactions were found to ...
0035] Generally stated, the non-limitative illustrative embodiment described hereinafter relates to a high sensitivity plasmonic structure for use in a surface plasmon resonance (SPR) sensor, and a method of fabrication thereof. The plasmonic structure comprises an array of microholes defining triangles of 700 nm, 950 nm and 1.8 μm edge lengths, which transition to propagating SPR with microhole arrays of decreasing size. Such microhole arrays exhibit a short range SPR mode (as measured in the Kretschmann configuration SPR). Triangle arrays of different sizes and aspect ratio generally exhibit two absorption bands and a transmission maximum in the SPR spectrum. The maximum in transmission at approximately λ=600 nm exhibits the best analytical characteristics for triangle arrays. This maximum shifts significantly with increasing refractive index (RI) for the triangles of 950 nm and 1.8 μm edge lengths, with a sensitivity of 1993 and 1038 nm/RI respectively. This high sensitivity is comparable ...
A side-polished multimode fiber sensor based on surface plasmon resonance (SPR) as the transducing element with a halogen light source is proposed. The SPR fiber sensor is side polished until half the core is closed and coated with a 37 nm gold thin film by dc sputtering. The SPR curve on the optical spectrum is described by an optical spectrum analyzer and can sense a range of widths in wavelengths of SPR effects. The measurement system using the halogen light source is constructed for several real-time detections that are carried out for the measurement of the index liquid detections for the sensitivity analysis. The sensing fiber is demonstrated with a series of refractive index (RI) liquids and set for several experiments, including the stability, repeatability, and resolution calibration. The results for the halogen light source with the resolution of the measurement based on wavelength interrogation were ...
1996 (English)In: 3: rd Meeting and Seminar on : Ceramics,Cells and Tissues, (Ed. Ravaglioli,A. and Krajewski,A.). Gruppo editoriale faenza editrice s.p.a., 1996, 171-178 p.Conference paper, Published paper (Other scientific) ...
Publikations-Datenbank der Fraunhofer Wissenschaftler und Institute: Aufsätze, Studien, Forschungsberichte, Konferenzbeiträge, Tagungsbände, Patente und Gebrauchsmuster
TY - JOUR. T1 - Enhancement of surface plasmon resonance sensing for DNA hybridization using colloidal Au attached probe DNA. AU - Yamaguchi, Akira. AU - Juodkazis, Saulius. AU - Matsuo, Shigeki. AU - Misawa, Hiroaki. PY - 2002/2/5. Y1 - 2002/2/5. N2 - In this study, we demonstrate that the Au particle modified probe DNA monolayer can enhance the surface plasmon resonance (SPR) signal for measuring hybridization of unlabeled DNA molecules. The Au particles adsorbed on single stranded (ss)- and double stranded (ds)-DNA monolayers have different optical interaction with surface of Au thin film, and this difference induces the enhancement of the SPR signal.. AB - In this study, we demonstrate that the Au particle modified probe DNA monolayer can enhance the surface plasmon resonance (SPR) signal for measuring hybridization of unlabeled DNA molecules. The Au particles adsorbed on single stranded (ss)- and double stranded (ds)-DNA monolayers have different optical interaction with surface of Au thin ...
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TY - JOUR. T1 - Differential roles of ionic, aliphatic, and aromatic residues in membrane - Protein interactions. T2 - A surface plasmon resonance study on phospholipases A2. AU - Stahelin, R. V.. AU - Cho, W.. PY - 2001/4/17. Y1 - 2001/4/17. N2 - The roles of cationic, aliphatic, and aromatic residues in the membrane association and dissociation of five phospholipases A2 (PLA2), including Asp-49 PLA2 from the venom of Agkistodon piscivorus piscivorus, acidic PLA2 from the venom of Naja naja atra, human group IIa and V PLA2s, and the C2 domain of cytosolic PLA2, were determined by surface plasmon resonance analysis. Cationic interfacial binding residues of A. p. piscivorus PLA2 (Lys-10) and human group IIa PLA2 (Arg-7, Lys-10, and Lys-16), which mediate electrostatic interactions with anionic membranes, primarily accelerate the membrane association. In contrast, an aliphatic side chain of the C2 domain of cytosolic PLA2 (Val-97), which penetrates into the hydrophobic core of the membrane and ...
Autor: Kobayashi, K. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2002-08; Titel: Monolayer formation of cytochrome b(562) on gold surfaces and its reconstitution reaction, studied by surface plasmon resonance spectroscopy
TY - JOUR. T1 - Sequential analysis of multiple analytes using a surface plasmon resonance (SPR) biosensor. AU - Chung, J. W.. AU - Bernhardt, R.. AU - Pyun, J. C.. PY - 2006/4/20. Y1 - 2006/4/20. N2 - A sequential analysis method for the analysis of two analytes was developed using a surface plasmon resonance (SPR) biosensor. A sample with both analytes was introduced into the single sensing region and then each analyte was analyzed sequentially. Two detection models were devised for the samples with the following composition: (1) one target analyte resulting in a sensor response without any label and the other analyte with only additional label, (2) both target analytes requiring additional labels for detection. A standard curve for each model was prepared and applied for sequential analysis of anti-bovine serum albumin (anti-BSA) antibodies and horseradish peroxidase (HRP). The errors of the sequential analysis of Models 1 and 2 were found to be less than 6%, and this method was therefore ...
Protein sequence and surface plasmon resonance analysis of the anti-IGFBP7 sdAb 4.43. (A) Protein sequence of anti-IGFBP7 sdAb 4.43; CDR1, CDR2 and CDR3 are und
en] Surface plasmon resonance (SPR)-based biosensors are very powerful tools for the study of biomolecular interactions, chemical detection and immunoassays. This paper reviews the performance of various SPR structures and detection schemes focusing on propagating surface plasmons generated in planar structures. Some aspects of their surface functionalization, the key element which imparts biofunctionality to these structures and hence transforming them into biosensors, will also be discussed accordingly. The ultimate performance of SPR-based biosensors will thus be determined by both their inherent optical performance and suitable surface functionalization. (C) 2011 Elsevier Ltd. All rights reserved ...
Using synthetic Tn (GalNAc-O-Ser/Thr) glycopeptide models and a biosensor based on surface plasmon resonance spectroscopy we have determined that isolectin B4 from Vicia villosa (VVLB4) binds to one Tn determinant whereas the anti-Tn monoclonal antibodies 83D4 and MLS128 require at least two Tn residues for recognition. When an unglycosylated amino acid is introduced between the Tn residues, both antibodies do not bind. MLS128 affinity was higher on a glycopeptide with three consecutive Tn residues. These results indicate that Tn residues organized in clusters are essential for the binding of these antibodies and indicate a different Tn recognition pattern for VVLB4.
Dr. Zeczycki from East Carolina University, uses OpenSPRs surface plasmon resonance technology to get the key binding data needed for their recent publication on protein-lipid interaction.
TY - JOUR. T1 - Surface plasmon resonance phase imaging measurements of patterned monolayers and DNA adsorption onto microarrays. AU - Halpern, Aaron R.. AU - Chen, Yulin. AU - Corn, Robert M.. AU - Kim, Donghyun. PY - 2011/4/1. Y1 - 2011/4/1. N2 - The optical technique of surface plasmon resonance phase imaging (SPR-PI) is implemented in a linear microarray format for real-time measurements of surface bioaffinity adsorption processes. SPR-PI measures the phase shift of p-polarized light incident at the SPR angle reflected from a gold thin film in an ATR Kretschmann geometry by creating an interference fringe image on the interface with a polarizer-quartz wedge depolarizer combination. The position of the fringe pattern in this image changes upon the adsorption of biomolecules to the gold thin film. By using a linear array of 500 μm biosensor element lines that are perpendicular to the interference fringe image, multiple bioaffinity adsorption measurements can be performed in real time. Two ...
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Bioelectron. 19, 1497-1504. Oh, B. , Chun, B. , Bae, Y. , Lee, W. , and Choi, J. W. (2005). The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosens. Bioelectron. 20, 1847-1850. , and Matsunaga, T. (1999). Electrochemical detection of allergen in small-volume whole blood using an array microelectrode: A simple method for detection of allergic reaction. Biotechnol. Bioeng. 65, 480-484. Okrend, A. J. , Rose, B. , and Lattuda, C. P. (1992). Isolation of Escherichia coli O157:H7 using O157 specific antibody coated beads. Lee, W. , and Choi, J. W. (2005). The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosens. Bioelectron. 20, 1847-1850. , and Matsunaga, T. (1999). Electrochemical detection of allergen in small-volume whole blood using an array microelectrode: A simple method for detection of allergic reaction. Biotechnol. Bioeng. 65, 480-484. Okrend, A. J. , Rose, B. , and Lattuda, C. P. (1992). Isolation ...
An integrated optical waveguide type surface plasmon resonance (SPR) sensor having an optical waveguide with a corresponding SPR sensing area, photodetectors, and wavelength tunable laser or any kind of external tunable laser source/coupler formed on a substrate. In an embodiment, the laser is a wavelength tunable laser and optionally, the integrated device may include a power source on the substrate for providing a electric power to the wavelength tunable laser and the photodetectors, or a circuit for signal processing, or a microfluidic structure for routing a target sample to the SPR sensor area. The microfluidic structure optionally includes a mixer or a reaction chamber for mixing and allowing a physical or chemical reaction to occur, respectively. In an embodiment, plural planar integrated optical waveguide type SPR sensors may be fabricated on a substrate to form an array of SPR sensors.
Surface Plasmon Resonance (SPR) is created by surface plasmons, coherent electron oscillations existing between any two materials where the real part of the dielectric function changes sign across the interface. SPR technology is based on the electromagnetic field component of incident light penetrating into a surface and it can be used to detect molecular adsorption on surfaces.. Plasmon excitation typically exists in two configurations: the Kretschmann-Raether configuration, where a thin metal film is sandwiched between a dielectric and air, and the incident wave is from the dielectric side; and the Otto configuration, where an air gap exists between the dielectric and the metal. Using COMSOL Multiphysics, the effect of the SPR on the electromagnetic field can be defined, which has allowed for the measurement of surface contaminants and nano-scale photonic devices.. ...
0082] SPRi detection of biomolecular-binding interactions was performed using the SPRi Lab+ apparatus equipped with an 800 nm LED source, CCD camera and a flow cell (GenOptics, France), placed in Memmert Peltier-cooled incubator (Rose Scientific, Canada) for temperature control (for detailed system specifications, see L. Malic, B. Cui, M. Tabrizian, T. Veres, Nanoimprinted plastic substrates for enhanced surface plasmon resonance imaging detection, Opt. Express, 17, 20386-92 (2009)). The entire biochip surface was imaged during the angular scan, while for each experiment three ˜500 μm diameter spots were selected for the monitoring of the binding interactions with both the probe and the control. For each spot, the reflected intensity was displayed as a function of angle in the plasmon curve diagram. The slope of the plasmon curves was automatically computed to facilitate the selection of the working angle for kinetic analysis, which corresponded to the point of the plasmon curve at which the ...
The simultaneous detection of multiple analytes is an important consideration for the advancement of biosensor technology. Currently, few sensor systems possess the capability to accurately and precisely detect multiple antigens. This work presents a simple approach for the functionalization of sensor surfaces suitable for multichannel detection. This approach utilizes self-assembled monolayer (SAM) chemistry to create a nonfouling, functional sensor platform based on biotinylated single-stranded DNA immobilized via a streptavidin bridge to a mixed SAM of biotinylated alkanethiol and oligo(ethylene glycol). Nonspecific binding is minimized with the nonfouling background of the sensor surface. A usable protein chip is generated by applying protein-DNA conjugates which are directed to specific sites on the sensor chip surface by utilizing the specificity of DNA hybridization. The described platform is demonstrated in a custom-built surface plasmon resonance biosensor. The detection capabilities of ...
Shifting of the surface plasmon resonance wavelength induced by the variation of the thickness of insulating spacer between single layer graphene and Au nanoparticles is studied. The system demonstrates a blue-shift of 29 nm as the thickness of the spacer layer increases from 0 to 15 nm. This is due to the electromagnetic coupling between the localized surface plasmons excited in the nanoparticles and the graphene film. The strength of the coupling decays exponentially with a decay length of d/R = 0.36, where d is the spacer layer thickness and R is the diameter of the Au nanoparticles. The result agrees qualitatively well with the plasmon ruler equation. Interestingly, a further increment of the spacer layer thickness induces a red-shift of 17 nm in the resonance wavelength and the shift saturates when the thickness of the spacer layer increases above 20 nm. © 2012 Optical Society of America ...
TY - JOUR. T1 - Fluorescence correlation spectroscopy in surface plasmon coupled emission microscope. AU - Borejdo, Julian. AU - Calander, N.. AU - Gryczynski, Zygmunt. AU - Gryczynski, Ignacy. PY - 2006/1/1. Y1 - 2006/1/1. N2 - Study of dynamics of single molecules by Fluorescence Correlation Spectroscopy (PCS) requires that the rate of photon detection per molecule be high, that the background be low, and that there be a large change in fluorescent signal associated with change in a position of a molecule. PCS applied to microscopic Surface Plasmon Coupled Emission (SPCE) suggests a powerful method to meet those requirements. In this method, the observational volume is made shallow by placing a sample on a thin metal film and illuminating it with the laser beam at Surface Plasmon Resonance (SPR) angle through high numerical aperture objective. The illuminating light excites surface plasmons in the metal film that produce an evanescent wave on the aqueous side of the interface. The thickness of ...
The development of a novel electrochemically stable host-guest supramolecular complex at a host surface is described. It was constructed by combining a self-assembled monolayer (SAM) of mono-(6-deoxy-6-mercapto)-beta-cyclodextrin (beta CDSH), iron (III) tetra-(N-methyl-4-pyridyl)-porphyrin (FeTMPyP) as the guest-link-molecule and beta-cyclodextrin-functionalized gold nanoparticles (beta CDAuNP). The building process of the layer-by-layer assembly was monitored by surface plasmon resonance spectroscopy (SPR). The binding processes between the host-functionalized gold surface and the linker molecule (FeTMPyP) were verified to be monovalent, and for host-functionalized gold nanoparticles with FeTMPyP, the interaction was determined to be bivalent. Finally, the electrochemical properties of the electroactive supramolecular multivalent film were determined. (C) 2009 Elsevier B.V. All rights reserved ...
In this study, we performed finite element method (FEM) simulations to optimize the configuration of gold nanorods (GNR) enhanced surface plasmon resonance (SPR) sensor and discovered its application for multiplex antigens detection. Our work analyzed the near-field coupling between the sensing film and GNR. By systematically study the effect of gold film thickness, GNR-to-film distance and GNR dimensions on SPR, it was found that for GNR width smaller than 40nm, length change in GNR brought about significant SPR wavelength shift on the sensor, while the sensor is insensitive for GNR-to-film distance. As an application, we adopted GNRs of width 20 nm and aspect ratios from 2 to 4 and demonstrated the concept of conjugating gold film and GNRs with anti-Immunoglobulin G (anti-IgG) antibodies for multiplex detection of various IgG proteins with more than 100nm separation on their SPR wavelengths ...
Nanoparticles have offered many diverse capabilities in bioanalysis and biotechnological applications, due to the intrinsic properties of nanoparticle that is distinguished from bulk materials. The integration of nanoparticle, which exhibit unique electronic, photonic, and catalytic properties with biomaterial, which display unique recognition, catalytic, and inhibition property yields novel hybrid nanobiomaterial with synergic properties and functions. In this study, gold (Au) nanoparticle-antibody conjugate was applied for the signal enhancement of surface plasmon resonance (SPR). When small molecule is a target for immobilization or detection, the changes in the angle-dependent attenuated total reflectance (ATR) are often small so the use of SPR method is limited for generic application. The specific interaction between target molecules and Au nanoparticleantibody conjugate induce the change of the surface properties such as mass and roughness, which can be represented as the change of plasmon
Pope ME, Soste MV, Eyford BA, Anderson NL, Pearson TW. Anti-peptide antibody screening: selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique. J Immunol Methods. 2009 Feb 28;341(1-2):86-96. Epub 2008 Nov 28. PMID: ...
Pope ME, Soste MV, Eyford BA, Anderson NL, Pearson TW. Anti-peptide antibody screening: selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique. J Immunol Methods. 2009 Feb 28;341(1-2):86-96. Epub 2008 Nov 28. PMID: ...
Surface Plasmon Resonance (SPR) is a powerful label free optical biosensing technology that relies on the measurement of the refractive index or change of mass in close vicinity of the sensor surface. Therefore, there is an experimental limitation in the molecular weight of the molecule that can be detected and consequently small molecules are intrinsically more difficult to detect using SPR. One approach to overcoming this limitation is to first adsorb smaller molecules onto the sensor surface, and to follow this by using their higher molecular weight antibodies counterparts which ensure the specificity (and are easier to detect via SPR due to their higher weight). Although this has been demonstrated with some success, it is not applicable in every case and some biomolecules such as enzyme are still difficult to detect due to their specific reactivity (enzymatic reaction). In this paper, we present a powerful new method that utilises specifically engineered spacers attached on one end to the ...
B-lymphocyte antigen CD22 is a member of the recently described sialoadhesin family of immunoglobulin-like cell-surface glycoproteins that bind glycoconjugates terminating in sialic acid. One prominent ligand for CD22 is the highly glycosylated leukocyte surface protein CD45. Using surface plasmon resonance spectroscopy, we characterized the interaction of recombinant mouse CD22 with native CD45 purified from rat thymus (CD45-thy). By in situ desialylation and resialylation of immobilized CD45-thy, we show that mouse CD22 binds to the sialoglycoconjugate NeuGc alpha 2-6Gal beta 1-4GlcNAc carried on CD45-thy N-glycans. Previous studies have shown that the sialic acid-binding site lies within the two membrane-distal domains of CD22 (domains 1 and 2), which are V-set and C2-set immunoglobulin superfamily domains, respectively. To further localize the binding site, we have made 42 single amino acid substitutions throughout both domains. All 12 mutations that abrogated binding to CD45-thy without disrupting
The human organism employs G protein coupled receptors (GPCRs), cell surface receptors in the plasma membrane, to transmit extracellular signals such as hormones, neurotransmitters, gustatory and olfactory signals into the interior of the cell. Specific binding of these extracellular ligands induces a conformational change in GPCRs, which allows the interaction with heterotrimeric G proteins and the catalysis of GDP/GTP nucleotide exchange in the G protein. The G protein then transmits the signal by protein-protein interaction to intracellular effector proteins. The publications summarized here on the rhodopsin/transducin system of photoreceptor cells, a model system for GPCRs/G proteins, cover three topics: 1. Methodology developments for biophysical monitoring of the interaction between rhodopsin and transducin by light scattering and evanescent field techniques, in particular surface plasmon resonance spectroscopy. 2. Formation of the active conformation of rhodopsin. The temporal sequence of ...
Original Research and Commentary on p28 CDG Therapeutics Lead Agent and Analogs. Binding of Amphipathic Cell Penetrating Peptide p28 to Wild Type and Mutated p53 as studied by Raman, Atomic Force and Surface Plasmon Resonance spectroscopies.. Signorelli S, Santini S, Yamada T, Bizzarri AR, Beattie CW, Cannistraro S.. Biochim Biophys Acta. 2017 Apr;1861(4):910-921.. Phase 1 trial of p28 (NSC745104), a non-HDM2-mediated peptide inhibitor of p53 ubiquitination in pediatric patients with recurrent or progressive central nervous system tumors: A Pediatric Brain Tumor Consortium Study.. Lulla RR, Goldman S, Yamada T, Beattie CW, Bressler L, Pacini M, Pollack IF, Fisher PG, Packer RJ, Dunkel IJ, Dhall G, Wu S, Onar A, Boyett JM, Fouladi M.. Neuro Oncol., 2016 Sep;18(9):1319-25. p28-mediated Activation of p53 in G2/M Phase of the Cell Cycle Enhances the Efficacy of DNA Damaging and Antimitotic Chemotherapy.. Yamada T, Das Gupta TK, Beattie CW.. Cancer Res. 2016 Feb 26; 76(8): 2354-2365. Chirality ...
A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real-time as a function of thromboplastin and heparin concentrations. The physical reason for the SPR signal observed is discussed and 3 different models are proposed. The response curves were analyzed by multivariable curve fitting followed by feature extraction. Interesting parameters of the sigmoid curves were lag time, slope and maximum response. When thromboplastin concentrations were increased, the lag-time decreased and the slope of the curve increased. A prolonged clotting time was followed mostly by increased maximum response, with exception for samples with no or very little thromboplastin added. High heparin concentrations changed the clotting kinetics. As seen from the lag-time vs. slope relation. Atomic force microscopy pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network structures as a function of thromboplastin concentration, and fiber ...
Abstract. Continuously monitoring cell cultures is essential for both controlling critical parameters and improving understanding of key processes. An ideal technique in this context is surface plasmon resonance (SPR) spectroscopy, which essentially exploits changes in the angle of incident light that occur when molecules bind to a surface. It provides the ability to monitor real-time changes in small concentrations of various molecules, with no need for additional labels or sample preparation. Here we present an SPR-based immunoassay for monitoring concentrations of human serum albumin (HSA), and compare its sensitivity when used in conjunction with a Biacore platform and the cheaper, smaller liSPR system. In conjunction with either system, the immunoassay can detect HSA (a hepatocyte viability marker) at concentrations typically present in three-dimensional hepatocyte cultures mimicking the liver used to evaluate effects of drug candidates before exposure to humans or animals. Furthermore, in ...
The CD8 coreceptor of cytotoxic T lymphocytes binds to a conserved region of major histocompatibility complex class I molecules during recognition of peptide-major histocompatibility complex (MHC) class I antigens on the surface of target cells. This event is central to the activation of cytotoxic T lymphocyte (CTL) effector functions. The contribution of the MHC complex class I light chain, beta(2)-microglobulin, to CD8alphaalpha binding is relatively small and is mediated mainly through the lysine residue at position 58. Despite this, using molecular modeling, we predict that its mutation should have a dramatic effect on CD8alphaalpha binding. The predictions are confirmed using surface plasmon resonance binding studies and human CTL activation assays. Surprisingly, the charge-reversing mutation, Lys(58) --| Glu, enhances beta(2)m-MHC class I heavy chain interactions. This mutation also significantly reduces CD8alphaalpha binding and is a potent antagonist of CTL activation. These results suggest a
Objective To identify the epitopes recognized by autoantibodies targeting platelet-derived growth factor receptor α (PDGFRα) in systemic sclerosis (SSc) and develop novel assays for detection of serum anti-PDGFRα autoantibodies. Methods Epstein-Barr virus-immortalized B cells from 1 patient with SSc (designated PAM) were screened for expression of IgG binding to PDGFRα and induction of reactive oxygen species in fibroblasts. The variable regions of anti-PDGFRα IgG were cloned into an IgG expression vector to generate distinct recombinant human monoclonal autoantibodies (mAb), which were characterized by binding and functional assays. The epitopes of anti-PDGFRα recombinant human mAb were defined by molecular docking, surface plasmon resonance binding assays, screening of a conformational peptide library spanning the PDGFRα extracellular domains, and expression analyses of alanine-scanned PDGFRα mutants. Direct or competitive enzyme-linked immunosorbent assays were established to detect ...
Calcium-calmodulin (CaM) binding to the epidermal growth factor receptor (EGFR) has been shown to both inhibit and stimulate receptor activity. CaM binds to the intracellular juxtamembrane (JM) domain (Met645-Phe688) of EGFR. Protein kinase C (PKC) mediated phosphorylation of Thr654 occurs within this domain. CaM binding to the JM domain inhibits PKC phosphorylation and conversely PKC mediated phosphorylation of Thr654 or Glu substitution of Thr654 inhibits CaM binding. A second threonine residue (Thr669) within the JM domain is phosphorylated by the mitogen-activated protein kinase (MAPK). Previous results have shown that CaM interferes with EGFR-induced MAPK activation. If and how phosphorylation of Thr669 affects CaM-EGFR interaction is however not known.In the present study we have used surface plasmon resonance (BIAcore) to study the influence of Thr669 phosphorylation on real time interactions between the intracellular juxtamembrane (JM) domain of EGFR and CaM. The EGFR-JM was expressed as ...
Gelatinase B (MMP-9) and galectin-3 are widely known to participate in tumor cell invasion and metastasis. Glycans derived from MMP-9 expressed in MCF-7 breast cancer and THP-1 myeloid leukemia cells were compared with those from MMP-9 expressed in natural neutrophils. The many O-linked glycans of neutrophil gelatinase B presented a cluster of mainly galactosylated core II structures, 46% of which were ligands for galectin-3; 11% contained two to three N-acetyllactosamine repeating units that are high-affinity ligands for the lectin. The glycan epitopes thus provide MMP-9 with both high-affinity and (presumably) high-avidity interactions with galectin-3. In contrast, the O-glycans released from MMP-9 expressed in MCF-7 and THP-1 cells were predominantly sialylated core I structures. Only 10% of MCF-7 and THP-1 gelatinase B O-glycans were ligands for galectin-3 and contained only a maximum single N-acetyllactosamine repeat. Consistent with the glycan analysis, surface plasmon resonance binding ...
Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance SPR Technique: A Novel Method to Assess Biomolecular Interactions. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Several proteins, like transcription factors, bind to certain DNA sequences, thereby regulating biochemical pathways that determine the fate of the corresponding cell. Due to these key positions, it is indispensable to analyze protein-DNA interactions and to identify their mode of action. Surface plasmon resonance is a label-free method that facilitates the elucidation of real-time kinetics of biomolecular interactions. In this article, we focus on this biosensor-based method and provide a detailed guide how SPR can be utilized to study binding of proteins to oligonucleotides. After a description of the physical phenomenon and the instrumental realization including fiber-optic-based SPR and SPR imaging, we will continue with a survey of immobilization methods. Subsequently, we will focus on the optimization of the experiment, expose pitfalls, and introduce how data should be analyzed and published. Finally, we summarize several interesting publications of the last decades dealing with ...
Although it has been revealed that astrocytes, generally known as star-shaped glial cells, play critical roles in the functions of central nervous system, there have been few efforts to directly modul... Tags: intracellular calcium, near infrared, localized surface plasmon resonance, astrocytes, gold nanorods.
Evaluation of cytochrome c affinity to anionic phospholipids by means of surface plasmon resonance. by German Stepanov, Oksana Gnedenko, Andrey Molnar, Alexis Ivanov, Yuri Vladimirov, Anatoly Osipov. FEBS letters. Read more related scholarly scientific articles and abstracts.
Animal Models, Confocal Microscopy, Directed Evolution, Fluorescence Microscopy, Surface Plasmon Resonance, Translation Research, rDNA Technology Biological Pathways, Biosensors and diagnostic agents, Cancer, Drug Delivery, Drug Development, Drug Discovery, G Protein-coupled Receptors, Gene Therapy, Immunomodulation, Immunotherapy, Intracellular Trafficking, Nanomedicine, Nanoparticle, Oncology. ...
A sensor is described which utilizes the phenomenon of surface plasmon resonance to detect changes in refractive index of chemical or biochemical samples applied to a surface modified optical fiber. The sensor is constructed by polishing a short section of the lateral surface of an optical fiber to its evanescent field surrounding the fiber core. One or more thin films are applied to the polished section of the fiber to produce the sensing element. One of the films is the metal silver, which acts as the support for the surface plasmon. Under the proper conditions, TM polarized energy propagating in the fiber can be coupled to a surface plasmon electromagnetic mode on the metal film. This coupling depends on the wavelength, the nature of the fiber, the refractive index and thickness of the thin films applied to the fiber, and the refractive index of a chemical sample in contact with the modified surface. The fiber to plasmon coupling is seen as a large attenuation of the light reaching the distal ...
TY - GEN. T1 - Evaluation of an affinity-amplified immunoassay of graphene oxide using surface plasmon resonance biosensors. AU - Chiu, Nan-Fu. AU - Huang, Teng Yi. AU - Kuo, Chun Chuan. PY - 2015/1/1. Y1 - 2015/1/1. N2 - We describe a fundamental study on the plasmonic properties and advanced biosensing mechanisms of functionalized graphene. We discuss a specific design using modified carboxyl groups, which can modulate surface plasmon (SP) coupling and provide an advantage for their binding to the sensing layer with high-performance affinity in an immunological reaction. The functionalized graphene-based surface plasmon resonance (SPR) biosensors have three advantages: high performance, high sensitivity, and excellent molecular kinetic response. In the future, functionalized graphene sheets will make a unique contribution to photonic and SPR diagnosis devices. We wish to highlight the essential characteristics of functionalized graphene-based SPR biosensors to assist researchers in developing ...
TY - JOUR. T1 - Ultra-high sensitivity of the non-immunological affinity of graphene oxide-peptide-based surface plasmon resonance biosensors to detect human chorionic gonadotropin. AU - Chiu, Nan Fu. AU - Kuo, Chia Tzu. AU - Lin, Ting Li. AU - Chang, Chia Chen. AU - Chen, Chen Yu. PY - 2017/8/15. Y1 - 2017/8/15. N2 - Specific peptide aptamers can be used in place of expensive antibody proteins, and they are gaining increasing importance as sensing probes due to their potential in the development of non-immunological assays with high sensitivity, affinity and specificity for human chorionic gonadotropin (hCG) protein. We combined graphene oxide (GO) sheets with a specific peptide aptamer to create a novel, simple and label-free tool to detect abnormalities at an early stage of pregnancy, a GO-peptide-based surface plasmon resonance (SPR) biosensor. This is the first binding interface experiment to successfully demonstrate binding specificity in kinetic analysis biomechanics in peptide aptamers ...
TY - JOUR. T1 - An improved immunoassay for detection of saxitoxin by surface plasmon resonance biosensors. AU - Yakes, B.J.. AU - Prezioso, S.. AU - Haughey, S.A.. AU - Campbell, Katrina. AU - Elliott, Christopher. AU - DeGrasse, S.L.. PY - 2011/8. Y1 - 2011/8. N2 - Saxitoxin and its analogs, the causative agents of paralytic shellfish poisoning (PSP), are a worldwide threat to seafood safety. Effective monitoring of potentially contaminated fishing areas as well as screening of seafood samples is necessary to adequately protect the public. While many analytical methods exist for detecting paralytic shellfish toxins (PSTs), each technique has challenges associated with routine use. One recently developed method [1] that overcomes ethical or performance-related issues of other techniques is the surface plasmon resonance (SPR) bioassay. Notwithstanding the advantages of this method, much research remains in optimizing the sensor substrate and assay conditions to create a robust technique for ...
TY - JOUR. T1 - Application of surface plasmon coupled emission to study of muscle. AU - Borejdo, J.. AU - Gryczynski, Z.. AU - Calander, N.. AU - Muthu, P.. AU - Gryczynski, I.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2006/10. Y1 - 2006/10. N2 - Muscle contraction results from interactions between actin and myosin cross-bridges. Dynamics of this interaction may be quite different in contracting muscle than in vitro because of the molecular crowding. In addition, each cross-bridge of contracting muscle is in a different stage of its mechanochemical cycle, and so temporal measurements are time averages. To avoid complications related to crowding and averaging, it is necessary to follow time behavior of a single cross-bridge in muscle. To be able to do so, it is necessary to collect data from an extremely small volume (an attoliter, 10 -18 liter). We report here on a novel microscopic application of surface plasmon-coupled emission (SPCE), which provides such a ...
TY - JOUR. T1 - Surface plasmon resonance and emitted light properties of polystyrene sphere films. AU - Shinbo, Kazunari. AU - Miyabayashi, Syunsuke. AU - Yoshizawa, Kazushi. AU - Shimizu, Hidehiko. AU - Kato, Keizo. AU - Kaneko, Futao. AU - Tanaka, Masato. AU - Wakamatsu, Takashi. AU - Advincula, Rigoberto C.. PY - 2003. Y1 - 2003. N2 - Polystyrene sphere films were fabricated and the optical properties were investigated using surface plasmon spectroscopy (SPS) and emitted light due to reverse irradiation utilizing SPS Kretschmann configuration. The films were fabricated using sphere dispersed solution with various diameters from about 100 to 300 nm. The morphologies of the fabricated films were observed using atomic force microscopy and the sphere films had almost three layer structure. In SPS curve, large dip and shallow broad dip were observed at around 75° and 50°, respectively. The dips were considered to be due to the three layer structure and some defects in the film. Furthermore, ...
Diarrhoea caused by enterotoxigenic Escherichia coli is one of the leading causes of mortality in children under five years of age and is a great burden on developing countries. The major virulence factor of the bacterium is the heat-labile enterotoxin (LT), a close homologue of the cholera toxin. The toxins bind to carbohydrate receptors in the gastrointestinal tract, leading to toxin uptake and, ultimately, to severe diarrhoea. Previously, LT from human- and porcine-infecting ETEC (hLT and pLT, respectively) were shown to have different carbohydrate-binding specificities, in particular with respect to N-acetyllactosamine-terminating glycosphingolipids. Here, we probed eleven single-residue variants of the heat-labile enterotoxin with surface plasmon resonance spectroscopy and compared the data to the parent toxins. In addition we present a 1.45 Å crystal structure of pLTB in complex with branched Lacto-N-neohexaose (Galbeta4GlcNAcbeta6[Galbeta4GlcNAcbeta3]Galbeta4Glc). The largest difference in
An integrated surface plasmon resonance sensor Welcome to the Integrated Sensor System Training Program Website. Sensing is inherently a multidisciplinary field, and those who seek to advance and exploit knowledge in this area have a wide range of backgrounds, including physics, chemistry, mechanical and electrical engineering, biomedical engineering and medicine. Although often well-trained in their own domain of expertise they are not always ready to transfer their skills to industry. Most students have little exposure to other related research domains and do not appreciate that sensors are complex systems, requiring biorecognition or chemical recognition elements, sample delivery components (e.g. microfluidics), transducing elements, signal amplification, signal processing and packaging, and that a multidisciplinary approach is essential. Sensor systems must address specific markets and are subject to regulatory control. Students often also lack knowledge in the key techniques of micro or nano
Plural circuit selection using role reversing control inputs | SYSTEM AND METHOD OF DATA COMMUNICATION USING TRELLIS CODED MODULATION OR TURBO TRELLIS CODED MODULATION IN... | Multiple bit memory cells and methods for reading non-volatile data | Two-dimensional blazed MEMS grating | Surface plasmon resonance sensor having real-time referencing |
TY - JOUR. T1 - Asp271 is critical for substrate interaction with the surface binding site in β-agarase A from Zobellia galactanivorans. AU - Wilkens, Casper. AU - Tiwari, Manish K.. AU - Webb, Helen. AU - Jam, Murielle. AU - Czjzek, Mirjam. AU - Svensson, Birte. PY - 2019. Y1 - 2019. N2 - In the marine environment agar degradation is assured by bacteria that contain large agarolytic systems with enzymes acting in various endo- and exo-modes. Agarase A (AgaA) is an endo-glycoside hydrolase of family 16 considered to initiate degradation of agarose. Agaro-oligosaccharide binding at a unique surface binding site (SBS) in AgaA from Zobellia galactanivorans was investigated by computational methods in conjunction with a structure/sequence guided approach of site-directed mutagenesis probed by surface plasmon resonance binding analysis of agaro-oligosaccharides of DP 4-10. The crystal structure has shown that agaro-octaose interacts via H-bonds and aromatic stacking along 7 subsites (L through R) of ...
Fcgamma receptors (FcgammaRs) are expressed on all immunologically active cells. They bind the Fc portion of IgG, thereby triggering a range of immunological functions. We have used surface plasmon resonance to analyze the kinetic and thermodynamic properties of the interactions between the ectodomains of human low affinity FcgammaRs (FcgammaRIIa, FcgammaRIIb, and FcgammaRIIIb-NA2) and IgG1 or the Fc fragment of IgG1. All three receptors bind Fc or IgG with similarly low affinities (K(D) approximately 0.6-2.5 microm) and fast kinetics, suggesting that FcgammaR-mediated recognition of aggregated IgG and IgG-coated particles or cells is mechanistically similar to cell-cell recognition. Interestingly, the Fc receptors exhibit distinct thermodynamic properties. Whereas the binding of the FcgammaRIIa and FcgammaRIIb to Fc is driven by favorable entropic and enthalpic changes, the binding of FcgammaRIII is characterized by highly unfavorable entropic changes. Although the structural bases for these
TY - JOUR. T1 - Investigation of the mechanism of binding between internalin B and heparin using surface plasmon resonance. AU - Hrtska, Sybil C Lang. AU - Kemp, Melissa M.. AU - Muñoz, Eva M.. AU - Azizad, Omaira. AU - Banerjee, Mani. AU - Raposo, Catarina. AU - Kumaran, Jyothi. AU - Ghosh, Partho. AU - Linhardt, Robert J.. PY - 2007/3/13. Y1 - 2007/3/13. N2 - Listeria monocytogenes, a food-borne pathogen that infects immunocompromised patients, enters and proliferates within mammalian cells by taking advantage of host cell machinery. While entry into macrophages and other phagocytic cells occurs constitutively, intracellular invasion of nonphagocytic cells, such as epithelial and endothelial cells, occurs through induced phagocytosis. Invasion of these nonphagocytic cell types is under the control of the secreted L. monocytogenes protein internalin B (InlB), which directly associates with and activates the receptor tyrosine kinase Met. Activation of Met by InlB has previously been shown to be ...
Biosensor technology is a powerful alternative to conventional techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diaberics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate ...
Nanoplasmonic sensors typically comprise arrangements of noble metal nanoparticles on a dielectric support. Thus they are intrinsically characterized by surface topography with corrugations at the 10-100 nm length scale. While irrelevant in some bio- and chemosensing applications, it is also to be expected that the surface topography significantly influences the interaction between solids, fluids, nanoparticles and (bio)molecules, and the nanoplasmonic sensor surface. To address this issue, we present a wafer-scale nanolithography-based fabrication approach for high-temperature compatible, chemically inert and topographically flat and laterally homogeneous nanoplasmonic sensor chips. We demonstrate their sensing performance on three different examples, for which we also carry out a direct comparison with a traditional nanoplasmonic sensor with representative surface corrugation. Specifically, we (i) quantify the film-thickness dependence of the glass transition temperature in poly(methyl metacrylate)
This paper reports the fabrication and testing of two configurations of optical sensor systems based on Surface Plasmon Resonance (SPR) at the interface of a liquid sample and sandwiched structures realized starting from the exposed core of a Plastic Optical Fiber (POF). The proposed geometries have proven to be suitable for measuring the refractive indexes of liquids whose refractive index falls around 1.35. Furthermore, the proposed sensing head, being low cost and relatively easy to realize, may be very attractive for biosensor implementation.
Angiopoietin-like 4 (ANGPTL4) is involved in angiogenesis and lipid metabolism. It is secreted by liver and adipose tissues and cleaved to generate circulating coiled-coil domain (CCD) and fibrinogen-like domain (FLD) fragments. The full-length ANGPTL4 produced by hypoxic endothelial cells interacts with the extracellular matrix (ECM). The ECM-bound and soluble forms of ANGPTL4 have antiangiogenic properties. We carried out a structure-function analysis to investigate the regulation of ANGPTL4 bioactivity in endothelial cells. We found that the recombinant CCD binds to the ECM, whereas the FLD is released into the medium. The CCD, like the full-length ANGPTL4, binds to heparan and dermatan sulfates in surface plasmon resonance assays and inhibits endothelial cell adhesion, motility, and tubule-like formation. In endothelial cells, ANGPTL4 is processed in the secretion medium after release from the ECM. This processing is altered by the proprotein convertases inhibitor alpha1-PDX and abolished by the
The purpose of this work was to study the effect of various process and formulation parameters on size and shapes of silver nanoparticles (AgNPs) based on surface plasmon resonance (SPR). AgNPs were prepared by chemical reduction using formaldehyde (HCHO) as reducing agent and capped by polyethylene glycol (PEG). Effect of several processing variables including the concentration and volume of capping agent and reaction time is reported. The size of monodispersed nanoparticles was between 30-100 nm and was stable for three months at both room temperature and 4°C ...
A novel biosensing approach for the label-free detection of nucleic acid sequences of short and large lengths has been implemented, with special emphasis on targeting RNA sequences with secondary structures. The approach is based on selecting 8-aminoadenine-modified parallel-stranded DNA tail-clamps as affinity bioreceptors. These receptors have the ability of creating a stable triplex-stranded helix at neutral pH upon hybridization with the nucleic acid target. A surface plasmon resonance biosensor has been used for the detection. With this strategy, we have detected short DNA sequences (32-mer) and purified RNA (103-mer) at the femtomol level in a few minutes in an easy and level-free way. This approach is particularly suitable for the detection of RNA molecules with predicted secondary structures, reaching a limit of detection of 50fmol without any label or amplification steps. Our methodology has shown a marked enhancement for the detection (18 for short DNA and 54 for RNA), when compared ...
OpenPlex, Horiba Scientifics redesigned manual label-free SPRi-Lab+ system, uses surface plasmon resonance imaging (SPRi) for real-time analysi
A Rutgers-led team has created better biosensor technology that may help lead to safe stem cell therapies for treating Alzheimers and Parkinsons diseases and other neurological disorders. The technology, which features a unique graphene and gold-based platform and high-tech imaging, monitors the fate of stem cells by detecting genetic material (RNA) involved in turning such cells into brain cells (neurons), according to a study in the journal Nano Letters.
Nanoplasmonics has raised much concern on tailoring the strong optical fields near noble metal nanostructures. In particular, surface-enhanced Raman Scattering (SERS) can take the full benefits of nanoplasmonics for providing one of the most highly sensitive biochemical sensing techniques. This sensitivity is used to identify biochemical molecules even at single molecule level. This technique does not require fluorescent labeling. For decades, thousands of plasmonic nanoparticles or nanostructures mounted on substrates have been reported for plasmonics-driven SERS. However, previous work overlooks consideration of the quantitative investigation between plasmon resonance and SERS signals. This oversight has been due to available methodology.. Universal correlations between plasmon resonance and SERS has recently been described by the research of Ki-Hun Jeong (see, Kang et al.1) in Journal Advanced Materials - This observation was successfully enabled by deformable nanoplasmonic membrane, i.e., ...
The obligate human pathogen Neisseria gonorrhoeae is responsible for the widespread sexually transmitted disease gonorrhoea, which in rare cases also leads to the development of disseminated gonococcal infection (DGI). DGI is mediated by PorBIA-expressing bacteria that invade host cells under low phosphate condition by interaction with the scavenger receptor-1 (SREC-I) expressed on the surface of endothelial cells. The interaction of PorBIA and SREC-I was analysed using different in vitro approaches, including surface plasmon resonance experiments that revealed a direct phosphate-independent high affinity interaction of SREC-I to PorBIA. However, the same binding affinity was also found for the other allele PorBIB, which indicates unspecific binding and suggests that the applied methods were unsuitable for this interaction analysis. Since N. gonorrhoeae was recently classified as a super-bug due to a rising number of antibiotic-resistant strains, this study aimed to discover inhibitors against ...
Generally, an immunoaffinity SPR biosensor detects a target analyte in a sample through highly selective adsorption by using the antigen–antibody interaction. For improving the sensitivity, various kinds of particles have been added to the already bound analytes on the SPR biosensor (sandwich assay). In this work, signal amplification was demonstrated by the expression of the IgG-binding Z-domain of protein A on the outer membrane of Escherichia coli via Autodisplay. The amount of Z-domain of protein A expressed on the outer membrane was calculated to be 280,000 molecules per cell. In addition, the IgGbinding ability of the expressed proteinwas characterized using FACS analysis. The signal amplification of the SPR biosensor was performed in the sandwich assay format using a model of horseradish peroxidase (HRP); the limit of detectionwas determined to be significantly improved from1 g/ml to 1 ng/ml. Finally, myoglobin analysis was demonstrated for the medical diagnosis of cardiac ...
Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.. A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.. When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.. The design of a sensor surface is important for the characteristics of a sensor. By binding ...
In order to overcome the sensitivity limitation of SPR, nanoparticle-coupled SPR biosensors have explored since nanoparticles may significantly enhance the sensitivity by 1-2 orders of magnitude. Although the nanoparticle conjugated method contributes to the enhancement of the sensitivity, it does not take an advantage of SPR from the viewpoint of label-free detection. Herein we demonstrate the sensitivity enhancement with Au nanoparticles coupled- SPR immuno-sensor chip on which the specific size and surface density of the particles controlled as a label-free detection system. Au nanopariticles were synthesized and selected with a specific size. Surface density of immobilized 30 nm Au nanoparticles on bare Au film was estimated as 1x109 ea/cm2 using atomic force microscopy. With systematic control of the size of the Au nanoparticle and thickness of the bare Au film, it was found that 30 nm Au particles on 50 nm thick Au film demonstrated the largest resonance angle shift for surface reaction on the
TY - JOUR. T1 - Toward surface plasmon polariton quantum-state tomography. AU - Dominguez, D.. AU - Regan, C. J.. AU - Bernussi, A. A.. AU - Grave De Peralta, L.. PY - 2013/2/21. Y1 - 2013/2/21. N2 - We report the direct excitation and detection of single-photon surface plasmon polariton (SPP) using a SPP tomography arrangement. Temporally spaced photons produced by spontaneous parametric downconversion were used to excite single-photon SPPs. The quantum statistics of the leakage radiation was studied using a Hanbury-Brown Twiss correlator arrangement. We observed a violation of the second order coherence test indicating leakage of temporally spaced photons. This demonstrates that leakage radiation associated with SPPs excited by single photons is composed of temporally spaced photons. Reaching the quantum regime of SPP tomography opens the door for further advances in SPP quantum state determination using SPP tomography.. AB - We report the direct excitation and detection of single-photon ...
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International Journal of Electrochemistry is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles that further our understanding of fundamental electrochemical processes, describe new electrochemical techniques, apply electrochemistry in analytical determination, or apply electrochemistry for chemical reactivity.
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Get this from a library! Narrow plasmon resonances in hybrid systems. [Philip A Thomas] -- Advances in understanding the interactions between light and subwavelength materials have enabled the author and his collaborators to tailor unique optical responses at the nanoscale. In particular, ...
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Mentors: Berron, Hastings. Surface-plasmon resonance (SPR) sensing is a widely used optical technique for detecting and analyzing biochemical interactions. It has found applications in diverse fields such as medical diagnostics, drug discovery, and environmental monitoring. A key challenge for SPR is maximizing sensitivity to a target analyte while minimizing response to interfering species. Dr. Hastings group has developed several SPR sensors that use optical methods to distinguish specific and non-specific interactions. Dr. Berrons group has developed stable, low-density self-assembled monolayers that are ideal for functionalizing the gold surfaces common to SPR sensors. The tunable density of exposed functional groups should allow optimization of the interaction with a chosen target, while suppressing non-specific protein binding. In addition, the SPR technique itself will increase our understanding of the kinetics of protein adsorption on low-density monolayers. Students working on this ...
TY - JOUR. T1 - The interaction of serum albumin with cholesterol containing lipid vesicles. AU - Meierhofer, T. AU - van den Elsen, J M H. AU - Cameron, Petra J. AU - Munoz-Berbel, X. AU - Jenkins, A T A. PY - 2010/1. Y1 - 2010/1. N2 - In this paper, the interaction of both human blood serum (the primary fraction of which is serum albumin) and pure human serum albumin (HSA) with surface immobilised lipid vesicles was measured by combined Surface Plasmon Resonance (SPR) and Surface Plasmon enhanced Fluorescence (SPEFS), and fluorescence microscopy. It was found that both blood serum and HSA showed specific binding to vesicles which contained cholesterol, resulting in increased membrane permeability and release of encapsulated fluorescent dye. This effect was not seen with heat inactivated blood serum, heat inactivated HSA or in vesicles not containing cholesterol. These results suggest that HSA may have a physiological role over and beyond that of fatty acid carrier, possibly acting to regulate ...
Biomolecular interaction analysis (BIA) using SPR (surface plasmon resonance) biosensors is now utilised increasingly in nearly all phases of drug development. The BIA system consists out of a light source emitting near infrared light, a sensor microchip, an automated liquid handling system with constant flow and a diode array position-sensitive detector. One of the two interacting partners (referred to as the ligand) is immobilized on the sensor surface. The other binding partner, called the analyte, is directed over the surface in a constant flow system allowing to monitor the interaction of the binding partners in real time ...
The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon resonance (SPR) that is more sensitive than the standard mouse bioassay for the quantification of BoNT/A activity. We have now adapted this procedure for pharmaceutical preparations. The optimized SPR assay allowed multiple measurements on a single chip, including the kinetics of substrate cleavage. The activity of five different batches of a pharmaceutical BoNT/A preparation was determined in a blind study by SPR and found to be in agreement with data from the in vivo mouse lethality assay. Biosensor detection of specific proteolytic products has the potential to accurately monitor the activity of pharmaceutical BoNT/A preparations, and a single chip can be used to assay more than 100 ...
Profacgen provides One-Stop-Service on protein-protein interaction analysis, including Yeast two-hybrid, Pull-downs and Surface Plasmon Resonance (SPR) assay etc., to facilitate your scientific research. Our service can be tailored according to your specific requirements.
Our laboratory improves magnetic resonance technology and spectroscopy for biomolecular structure determination relevant to HIV cure research. Solid state nuclear magnetic resonance (NMR) is exquisitely suited to probe atomic level structural detail of biomolecules within intact human cells. However, the small nuclear magnetic moments that yield narrow resonances also result in very low inherent sensitivity in NMR. Dynamic Nuclear Polarization (DNP) is a powerful combined electron paramagnetic resonance (EPR) and NMR technique that transfers the strong polarization from unpaired electron spins to nuclear spins to boost NMR sensitivity. In our DNP experiments, we employ frequency agile gyrotrons, and extreme cryogenic sample cooling to increase the sensitivity of solid state NMR experiments up to a factor of 20,000. This tremendous gain in sensitivity results in acquiring data 400 million times faster than conventional NMR experiments and will have a profound impact on magnetic resonance ...
Waltham, MA, January 2011 - Nova Biomedical today announced that, in response to rapid growth in its diabetes and whole blood point-of-care testing products business, it has purchased an additional 80,000 square-foot manufacturing/warehouse facility in Billerica, MA. According to Lou Borrelli, Nova Biomedical CFO, This additional state of the art manufacturing facility will ensure that our manufacturing capabilities keep pace with the increasing demand for our StatStrip Hospital Glucose products as well as our Nova Max consumer diabetes products.. One of the main drivers for Novas strong growth is the rapid adoption of its StatStrip Hospital Glucose Monitoring System. Since its inception just four years ago, StatStrip has become the fastest growing hospital glucose meter in the world. StatStrip uses a novel glucose test strip technology that measures hematocrit and other common interferences such as maltose, galactose, xylose, acetaminophen, ascorbic acid and oxygen, and eliminates erroneous ...
The most rapid and reliable way of detecting HIV is to search for the HIV genetic material directly in blood, using a process called PCR (polymerase chain reaction). This has the advantage of not needing to wait for such a long time after exposure, being used after only 3 days. Traditionally this process, known as molecular diagnostics, has been performed in a laboratory by trained personnel. Recent technological advances have however brought the possibility of conducting molecular diagnostics out of the laboratory and next to the patient at the point-of-care ...
Researchers at Harvard University have developed a non-toxic wearable sensor consisting of a silicon-rubber that attaches to the hand and capable of mearin | Technology
The overall learning outcome of the programme is that successful students will be able to demonstrate mastery in judgement and problem solving skills necessary to produce the highest quality diagnostic medical images using a variety of magnetic resonance technology systems.
Other methods include surface plasmon resonance (SPR), protein microarrays, dual polarisation interferometry, microscale ... Visser, NF; Heck, AJ (June 2008). "Surface plasmon resonance mass spectrometry in proteomics". Expert Review of Proteomics. 5 ( ... de Mol, NJ (2012). "Surface plasmon resonance for proteomics". Chemical Genomics and Proteomics. Methods in Molecular Biology. ...
... its linear optical response is mainly controlled by the surface plasmon resonance. Surface plasmon resonance occurs when the ... Platinum NPs synthesized via citrate reduction do not have a surface plasmon resonance peak around 215 nm. Through ... Mayer, K. M; Hafner, J. H (2011). "Localized surface plasmon resonance sensors". Chemical Reviews. 111 (6): 3828-57. doi: ... Willets, Katherine A; Van Duyne, Richard P (2007). "Localized Surface Plasmon Resonance Spectroscopy and Sensing". Annual ...
Schasfoort, R.B.M. (2017). Handbook of Surface Plasmon Resonance (2nd ed.). Royal Society of Chemistry. p. 296. ISBN ... The surface of the Scavenger container or channel has "Scavenger Antigens" bound to it. These can be identical or sufficiently ... In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody ... The steps are: A surface is prepared to which a known quantity of capture antibody is bound. Any nonspecific binding sites on ...
"Weak value amplification of an off-resonance Goos-Hänchen shift in a Kretschmann-Raether surface plasmon resonance device". ... In order to generate a giant Goos-Hänchen shift up to 100 μm, surface plasmon resonance techniques were applied based on an ... X. Yin (2006). "Goos-Hänchen shift surface plasmon resonance sensor". Applied Physics Letters. 89: 261108. doi:10.1063/ ... Both waves are reflected from the surface and undergo different phase shifts, which leads to a lateral shift of the finite beam ...
Laroy W, Ameloot P, Contreras R (2001). "Characterization of sialyltransferase mutants using surface plasmon resonance". ... 1990). "The B cell antigen CD75 is a cell surface sialytransferase". J. Exp. Med. 172 (2): 641-3. doi:10.1084/jem.172.2.641. ... 1992). "The HB-6, CDw75, and CD76 differentiation antigens are unique cell-surface carbohydrate determinants generated by the ... is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76 ...
NNV Najaarsvergadering 2013 Iu, H.; Li, J.; Ong, H. C.; Wan, J. T. K. (2008). "Surface plasmon resonance in two-dimensional ... and their inner surface was oxidized in the process. Then the Al matrix, but not the oxidized aluminium, was etched away by the ...
... probes Microphysiometry Multi-parametric surface plasmon resonance Plasmon Small molecule sensors Surface plasmon resonance Bio ... such that binding of a target analyte to a receptor on the gold surface produces a measurable signal. Surface plasmon resonance ... 2007). "A localized surface plasmon resonance based immunosensor for the detection of casein in milk". Sci. Technol. Adv. Mater ... Many optical biosensors are based on the phenomenon of surface plasmon resonance (SPR) techniques. This utilises a property of ...
"Complement C3b interactions studied with surface plasmon resonance technique". International Immunopharmacology. 1 (3): 495-506 ... it exposes a reactive thioester that allows the peptide to covalently attach to any surface that can provide a nucleophile such ...
March 2001). "Complement C3b interactions studied with surface plasmon resonance technique". International Immunopharmacology. ... on pathogen surfaces ensuring that self surfaces are protected whilst complement proceeds unabated on foreign surfaces. The ... Factor H exerts its protective action on self cells and self surfaces but not on the surfaces of bacteria or viruses. This is ... Factor H regulates complement activation on self cells and surfaces by possessing both cofactor activity for the Factor I ...
"Dynamically Modulating the Surface Plasmon Resonance of Doped Semiconductor Nanocrystals". Nano Letters. 11 (10): 4415-4420. ... "Dynamically Modulating the Surface Plasmon Resonance of Doped Semiconductor Nanocrystals". Nano Letters. 11 (10): 4415-4420. ... "Exceptionally Mild Reactive Stripping of Native Ligands from Nanocrystal Surfaces by Using Meerwein's Salt". Angewandte Chemie ...
Another method to be considered is Surface Plasmon Resonance (SPR). SPR can detect binding of label free molecules on the ... These resins have ligands attached to their surfaces which are specific for the compounds to be separated. Most frequently, ... allowing for separation of proteins based on their surface hydrophobicity. Target proteins and their product aggregate species ... surface of a chip. If the desired protein is an antibody, binding can be translated directly to the activity of the protein. ...
It competes with technologies including surface plasmon resonance. Recently, electrical stimulus has also been utilized to ... "Aptamer based electrostatic-stimuli responsive surfaces for on-demand binding/unbinding of a specific ligand". Journal of ... influence the binding between a protein and its ligand which could be incorporated into smart surfaces for controlled molecular ...
Chen, Hsiang-An; Lin, Hsin-Yu; Lin, Heh-Nan (June 17, 2010). "Localized Surface Plasmon Resonance in Lithographically ... uses a heatable scanning probe in order to efficiently remove material from a surface without the application of significant ... or according to the driving mechanisms of the probe-surface interaction used in the patterning process: mechanical, thermal, ... Transitioning from Field Induced Oxidation to Solvent Decomposition through Surface Modification". Advanced Materials. 19 (21 ...
Sun, Tong; Grattan, KTV (2014-05-01). "Gold nanorod-based localized surface plasmon resonance biosensors: A review". Sensors ...
Laich A, Sim RB (Jan 2001). "Complement C4bC2 complex formation: an investigation by surface plasmon resonance". Biochimica et ... The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha ...
Oh, J.; Choi, J. (2009). "Tunable color filter with surface plasmon resonance using organic photorefractive composite". Applied ... leads to the absorption of wavelengths generated by surface plasmon resonance and the reflection of complementary wavelengths. ...
Surface Plasmon Resonance (SPR) does not require labeling of the ligand. Instead, it works by measuring the change in the angle ... and surface plasmon resonance (SPR). In order to measure process of ligand-receptor binding, most non-radioactive methods ... Fluorescence Resonance Energy Transfer (FRET) utilizes energy transferred between the donor and the acceptor molecules that are ... Soaking the filter in a solution that would give the filter a positive surface charge would attract the negatively charged ...
... to be easily converted into an anti-fouling surface. Surface plasmon resonance (SPR) sensors are thin film-refractometers that ... Homola, Jiří (2008). "Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species". Chemical Reviews. ... High surface energies cause adsorption because a contaminated surface will have a smaller difference between the surface and ... Bondi, A. (1953). "The Spreading of Liquid Metals on Solid Surfaces. Surface Chemistry of High-Energy Surfaces". Chemical ...
"Measurement of small displacement based on surface plasmon resonance heterodyne interferometry". Optics and Lasers in ... "Common-path spectral interferometry with temporal carrier for highly sensitive surface plasmon resonance sensing". Optics ... "Testing Curved Surfaces and Lenses" (PDF). University of Arizona College of Optical Sciences. Archived from the original (PDF) ... It is normally used without any coating on either surface, so as to minimize ghost reflections. An aberrated wavefront from a ...
... characterization with surface plasmon resonance". Biol. Chem. 381 (3): 265-8. doi:10.1515/BC.2000.034. PMID 10782998. S2CID ...
Surface plasmon resonance sensors can be thin-film refractometers or gratings that measure the resonance behaviour of surface ... Surface plasmon resonance has been used in food quality and safety analysis, medical diagnostics, and environmental monitoring ... Homola, Jiří (2008). "Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species". Chemical Reviews. ... plasmon on metal or dielectric surfaces. The resonance changes when biomolecules are captured or adsorbed on the sensor surface ...
"Phosphorus-Doped Silicon Nanocrystals Exhibiting Mid-Infrared Localized Surface Plasmon Resonance". Nano Letters. 13 (3): 1317- ... Mostafa El-Sayed G. N. Lewis Michael Kasha Paul Alivisatos Localized surface plasmon Colloidal gold Scale invariance "Carbon- ... Jain is best known for discoveries and insights into plasmon resonances - collective electron oscillations in metal ... induced by light excitation and the applications of plasmon resonances in biomedicine, optoelectronics, and chemical catalysis ...
"Direct analysis of a GPCR-agonist interaction by surface plasmon resonance". Eur Biophys J. 35 (8): 709-712. doi:10.1007/s00249 ... It can be purified and analysed in vitro and has been analysed by a number of biophysical techniques such as surface plasmon ... resonance, FRET and cryo-electron microscopy. Furthermore, high-resolution crystal structures have been determined for NTS1 in ...
It can be performed using commercially available equipment utilising surface plasmon resonance. The surface of the sensor chip ... Whilst purification is not necessary for multitarget ELISA, it is essential for measuring binding affinity by plasmon resonance ... The association rate (kon) is calculated by measuring the rate of ZFP binding to the surface using several different protein ... Barbas CF, Kang AS, Lerner RA, Benkovic SJ (1991). "Assembly of combinatorial antibody libraries on phage surfaces: the gene ...
Multi-Parametric Surface Plasmon Resonance was used to characterize both thickness and refractive index of chemical-vapor- ... Jussila, Henri; Yang, He; Granqvist, Niko; Sun, Zhipei (5 February 2016). "Surface plasmon resonance for characterization of ... Lin, Xiao; Xu, Yang; Zhang, Baile; Hao, Ran; Chen, Hongsheng; Li, Erping (2013). "Unidirectional surface plasmons in ... Furthermore, the existence of unidirectional surface plasmons in the nonreciprocal graphene-based gyrotropic interfaces has ...
Zeng S, Yu X, Law W, Zhang Y, Hu R, Dinh X, H o H, Yong K (2013). "Size dependence of Au NP-enhanced surface plasmon resonance ... Ghosh SK, Nath S, Kundu S, Esumi K, Pal T (2004-09-01). "Solvent and Ligand Effects on the Localized Surface Plasmon Resonance ... Gold nanorods are rod-shaped gold nanoparticles whose aspect ratios tune the surface plasmon resonance (SPR) band from the ... The angle of the incidence light for surface plasmon resonance, an interaction between light wave and conducting electrons in ...
"Dendrimer-functionalized self-assembled monolayers as a surface plasmon resonance sensor surface". Langmuir. 20 (16): 6808-17. ... Secondary and tertiary amino surface groups are also found to be less toxic than primary amino surface groups, suggesting it is ... which allow many alterations to be made to the surface of each dendrimer molecule. In putative PAMAM dendrimers, the surface is ... any defects caused by incomplete reaction or intramolecular coupling of new surface amines with unreacted methyl ester surface ...
Surface plasmon resonance-based instruments use refractometry to measure and analyze biomolecular interactions. Polarization ... Polarimeter for measuring dispersion or rotation of polarized light Reflectometer for measuring the reflectivity of a surface ...
Conversely, localized surface plasmon resonances observed in covellite nanoparticles have recently been linked to the ... "Room-Temperature Synthesis of Covellite Nanoplatelets with Broadly Tunable Localized Surface Plasmon Resonance". Chemistry of ... As a secondary mineral, covellite also forms as descending surface water in the supergene enrichment zone oxidizes and ... The basal planes experience preferential oxygen adsorption and larger surface area facilitates electron transfer. In contrast, ...
The gold particles are red in color due to localized surface plasmon resonance. Fluorescent or magnetic labelled particles can ... Weijin Guo; Jonas Hansson; Wouter van der Wijngaart (2018). "Capillary pumping independent of the liquid surface energy and ... Jonas Hansson; Hiroki Yasuga; Tommy Haraldsson; Wouter van der Wijngaart (2016). "Synthetic microfluidic paper: high surface ... these tests run the liquid sample along the surface of a pad with reactive molecules that show a visual positive or negative ...
... localized surface plasmons are excited. The field enhancement is greatest when the plasmon frequency, ωp, is in resonance with ... The choice of surface metal is also dictated by the plasmon resonance frequency. Visible and near-infrared radiation (NIR) are ... Surface-enhanced Raman spectroscopy or surface-enhanced Raman scattering (SERS) is a surface-sensitive technique that enhances ... the plasmon oscillations must be perpendicular to the surface; if they are in-plane with the surface, no scattering will occur ...
Center for Surface Plasmon Resonance. *Center for Violence Prevention. *Children's Cancer Research Institute ...
dissociation constant values) between TCR and peptide/MHC was determined by the surface plasmon resonance (SPR) to be in the ... The T-cell receptor, or TCR, is a molecule found on the surface of T cells, or T lymphocytes,[1] that is responsible for ... "Selection of functional T cell receptor mutants from a yeast surface-display library". Proceedings of the National Academy of ... to their antigens despite the low affinity of TCR for its peptide/MHC and low numbers of specific peptide/MHC on the surface of ...
... but based on the enhancements for imaging with surface plasmons. In another direction researchers explored paraxial ... If the rings were closed, a half wavelength boundary would be electromagnetically imposed as a requirement for resonance.[5] ... 1996). "Extremely Low Frequency Plasmons in Metallic Microstructures". Phys. Rev. Lett. 76 (25): 4773-4776. Bibcode:1996PhRvL.. ... The individual SRR depicted on the right had a resonant frequency of 4.845 GHz, and the resonance curve, inset in the graph, is ...
Surface enhanced Raman spectroscopy. *Surface plasmon. *Surface plasmon polaritons. *Surface plasmon resonance ...
By contrast, inelastic X-ray scattering methods are useful in studying excitations of the sample such as plasmons, crystal- ... and the resonance observed in the planar carbonate group[31] and in aromatic molecules.[47] Kathleen Lonsdale's 1928 structure ... "Surface chemistry of phase-pure M1 MoVTeNb oxide during operation in selective oxidation of propane to acrylic acid". Journal ... this finding led to the idea of resonance between chemical bonds, which had profound consequences for the development of ...
... nuclear magnetic resonance (NMR)[edit]. Also in chemistry, the term "proton NMR" refers to the observation of hydrogen-1 ... The Apollo Lunar Surface Experiments Packages (ALSEP) determined that more than 95% of the particles in the solar wind are ... Such a bond happens at any sufficiently "cold" temperature (i.e., comparable to temperatures at the surface of the Sun) and ... nuclei in (mostly organic) molecules by nuclear magnetic resonance. This method uses the spin of the proton, which has the ...
Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein NMR. *Cryo-electron microscopy ... These two classes of spectral variants are often employed for Förster resonance energy transfer (FRET) experiments. Genetically ...
Here, it is shown that negative index is achieved by excitation of Surface Plasmon Polariton waves operating in dispersion ... Dillu, Venus; Sinha, R. K. (2013). "Enhanced Fano resonance in silver ellipsoidal plasmonic crystal cavity". Journal of Applied ... Plasmonics & Plasmonic Bandgap Engineering: Surface Plasmon Polaritons (SPPs) are electromagnetic waves guided along metal ... Dillu, Venus; Sinha, R. K. (2013). "Surface Plasmon Polariton Band Gap-Enabled Plasmonic Mach-Zehnder Interferometer: Design, ...
... such as surface plasmon resonance, dual polarization interferometry and Multi-Parametric Surface Plasmon Resonance (MP-SPR) can ... Multi-parametric surface plasmon resonance. Other techniques include: fluorescence intensity, bimolecular fluorescence ... complementation, FRET (fluorescent resonance energy transfer) / FRET quenching surface plasmon resonance, bio-layer ... "Heterobivalent ligands target cell-surface receptor combinations in vivo". Proceedings of the National Academy of Sciences of ...
Several types of optical fibre surface plasmon resonance (SPR) sensor are used for the point-contact detection of hydrogen: * ... Pd-coated nanomechanical resonators have also been reported in literature, relying on the stress-induced mechanical resonance ... accelerating the hydrogen oxidation reaction on the surface, which makes the sensor response much faster. The role of "matrix" ...
Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein NMR. *Cryo-electron microscopy ... Molecular surface of several proteins showing their comparative sizes. From left to right are: immunoglobulin G (IgG, an ... Resonance structures of the peptide bond that links individual amino acids to form a protein polymer ... The peptide bond has two resonance forms that contribute some double-bond character and inhibit rotation around its axis, so ...
Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein NMR. *Cryo-electron microscopy ...
"Surface plasmon resonance detection of E. coli and mathicillin-resistant S. aureus bacteriophages". Elsevier. 3 (5): e78. doi: ... Phage display is a different use of phages involving a library of phages with a variable peptide linked to a surface protein. ... After making contact with the appropriate receptor, the tail fibers flex to bring the base plate closer to the surface of the ... The technology for phages to be applied to dry surfaces, e.g., uniforms, curtains, or even sutures for surgery now exists. ...
Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein NMR. *Cryo-electron microscopy ... First, non-specific binding is not limited to the antibody-binding sites on the immobilized support; any surface of the ... In a bead-to-bead comparison, agarose beads have significantly greater surface area and therefore a greater binding capacity ... and antibody binding is limited to the surface of each bead. While these beads do not have the advantage of a porous center to ...
The term unstable molecule is used for very reactive species, i.e., short-lived assemblies (resonances) of electrons and nuclei ... "must correspond to a depression on the potential energy surface that is deep enough to confine at least one vibrational state". ... Nuclear resonance spectroscopy actually measures the environment of particular nuclei in the molecule, and can be used to ...
Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein NMR. *Cryo-electron microscopy ... as butanol removes bubbles and makes the surface smooth. [13] A source of free radicals and a stabilizer, such as ammonium ... Silver staining was developed in the 14th century for colouring the surface of glass. It has been used extensively for this ...
Surface-enhanced resonance Raman spectroscopy (SERRS) - A combination of SERS and resonance Raman spectroscopy that uses ... Surface plasmon polariton enhanced Raman scattering (SPPERS) - This approach exploits apertureless metallic conical tips for ... Surface plasmons of silver and gold are excited by the laser, resulting in an increase in the electric fields surrounding the ... 2012). "Surface plasmon polariton compression through radially and linearly polarized source". Optics Letters. 37 (4): 545. ...
... especially when surface plasmon resonance is involved (Roqué et al. 2006). Models of light scattering can be divided into three ... Surfaces described as white owe their appearance to multiple scattering of light by internal or surface inhomogeneities in the ... Highly scattering surfaces are described as being dull or having a matte finish, while the absence of surface scattering leads ... More generally, the gloss (or lustre or sheen) of the surface is determined by scattering. ...
... and liquid-gas surfaces. Multi-Parametric Surface Plasmon Resonance works in solid-gas, solid-liquid, liquid-gas surfaces and ... surface states, surface diffusion, surface reconstruction, surface phonons and plasmons, epitaxy, the emission and tunneling of ... Jussila, Henri; Yang, He; Granqvist, Niko; Sun, Zhipei (5 February 2016). "Surface plasmon resonance for characterization of ... It includes the fields of surface chemistry and surface physics.[1] Some related practical applications are classed as surface ...
Recently, cathodoluminescence performed in electron microscopes is also being used to study surface plasmon resonances in ... Surface plasmons in metal nanoparticles can absorb and emit light, though the process is different from that in semiconductors ... Cathodoluminescence plasmon microscopy [PDF], M. Kuttge, Utrecht University (2009). *Electron beams set nanostructures aglow [ ... A familiar example is the generation of light by an electron beam scanning the phosphor-coated inner surface of the screen of a ...
Lin, Feng; Markus, Isaac M.; Nordlund, Dennis; Weng, Tsu-Chien; Asta, Mark D.; Xin, Huolin L.; Doeff, Marca M. (2014). "Surface ... Krivanek, Ondrej L.; Lovejoy, Tracy C.; Dellby, Niklas; Aoki, Toshihiro; Carpenter, R. W.; Rez, Peter; Soignard, Emmanuel; Zhu ... allowing features such as plasmons, and elemental ionization edges to be identified. Energy resolution in EELS is sufficient to ...
Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein NMR. *Cryo-electron microscopy ... MST allows measurement of interactions directly in solution without the need of immobilization to a surface (immobilization- ...
At the surface of a metal resonance effects known as surface plasmons can result. They are collective oscillations of the ... and the plasmon resonance causes an extremely intense absorption in the green with a resulting beautiful purple-red color. Such ... the plasmon frequency. At the plasmon frequency, the frequency-dependent dielectric function of the free electron gas goes from ... Okumura, K. & Templeton, I. M. (1965). "The Fermi Surface of Caesium". Proceedings of the Royal Society of London A. 287 (1408 ...
... exhibits a size and shape dependent optical effect known as localized surface plasmon resonance (LSPR) at the nanoscale, the ... Nanoparticles synthesized in the substrate exhibit surface plasmon resonances as evidenced by characteristic absorption bands; ... studies investigating the oxidative catalytic enhancement of a nanostructure via excitation of its surface plasmon resonance. ... After implantation on the surface, the beam currents may be raised as the surface conductivity will increase.[41] The rate at ...
Localized surface plasmon. *Long-slit spectroscopy. *Lorentz-Lorenz equation. *Loupe light. M. *Madeira Optics Museum ... Morphology-dependent resonance. *Multipass spectroscopic absorption cells. *Multiphoton lithography. *Multiple-prism dispersion ...
Scanning tunneling microscope image showing the individual atoms making up this gold (100) surface. The surface atoms deviate ... Magnetic Resonance Imaging. vol. 2. John Wiley & Sons. pp. 412-426. ISBN 978-0-471-13946-1. .. ... At the surface of the Earth, an overwhelming majority of atoms combine to form various compounds, including water, salt, ... The scanning tunneling microscope is a device for viewing surfaces at the atomic level. It uses the quantum tunneling ...
... is due to the excitation of localized surface plasmons which creates strong absorption in a broad range in plasmon resonance. ... A surface plating of blue gold on karat gold or sterling silver can be achieved by a gold plating of the surface, followed by ... The broadness of the plasmon resonance, and absorption wavelength range, depends on the interaction between different gold ... A femtosecond laser pulse deforms the surface of the metal, creating an immensely increased surface area which absorbs ...
Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein NMR. *Cryo-electron microscopy ... Monoclonal antibodies, due to their size, are utilized against cell surface targets. Among the overexpressed targets are ...
Hydrogen sensor Multi-parametric surface plasmon resonance Nano-optics Plasmon Spinplasmonics Surface plasmon polariton Waves ... supporting surface electromagnetic waves with sharper resonances (Bloch surface waves). If the surface is patterned with ... In multi-parametric surface plasmon resonance, two SPR curves are acquired by scanning a range of angles at two different ... Multi-parametric surface plasmon resonance, a special configuration of SPR, can be used to characterize layers and stacks of ...
The surface plasmon resonance microscopy is based on surface plasmon resonance and recording desired images of the structures ... Surface plasmon resonance microscopy (SPRM), also called surface plasmon resonance imaging (SPRI), is a label free analytical ... Handbook of Surface Plasmon Resonance: Surface Plasmon Fluorescence Techniques for Bioaffinity Studies. RSC Publishing. Baba, A ... Tang, Y; Zeng, X; Liang, J (2010). "Surface Plasmon Resonance: An Introduction of a Surface Spectroscopy Technique". Journal of ...
... is an optical resonanceeffect where the backside of a thin conductive mirror affects the angle at ... Surface plasmon resonance is an optical resonance effect where the backside of a thin conductive mirror affects the angle at ... Lopez R, Haynes TE, Boatner LA, Feldman LC and Haglund RF Jr (2002) Temperature‐controlled surface plasmon resonance in VO 2 ... Surface Plasmon Resonance. Matthew J Fivash, National Cancer Institute, Frederick, Maryland, USA ...
Development Equipment Sensors Other Biosensor Tools Drug Discovery Label Free Sensing Life Sciences Surface Plasmon Resonance ... Other Antibodies Biosensor Tools Drug Discovery Life Sciences Lipids Protein Binding Studies Reagents Surface Plasmon Resonance ... Microscopes Scanning Electron Microscope Scanning Probe Microscopes Other Particle Sizing Products Surface Plasmon Resonance ... High-Throughput Surface Plasmon Resonance ...
Surface plasmon resonance imaging studies of protein-carbohydrate interactions.. Smith EA1, Thomas WD, Kiessling LL, Corn RM. ... arrays fabricated on gold films were used to study carbohydrate-protein interactions with surface plasmon resonance (SPR) ... were obtained for jacalin adsorbing to a galactose surface and ConA adsorbing to a mannose surface, respectively. The solution ... An immobilization scheme consisting of the formation of a surface disulfide bond was used to attach thiol-modified ...
With the emergence of surface plasmon resonance (SPR) arrays, investigators now have high-throughput capabilities to accelerate ... Home Webinars On Demand Webinars Accelerating Antibody Screening with Array-Based Surface Plasmon Resonance ... Accelerating Antibody Screening with Array-Based Surface Plasmon Resonance. January 17, 2018. 0 ... With the emergence of surface plasmon resonance (SPR) arrays, investigators now have high-throughput capabilities to accelerate ...
S. Herminjard, Mid-infrared surface plasmon resonance sensing applied to refractive index measurements of liquids, Ph.D. thesis ... Calibration of Surface Plasmon Resonance Imager for Biochemical Detection. T. Ktari, H. Baccar, M. B. Mejri, and A. Abdelghani ... C. A. Meeusen, E. C. Alocilja, and W. N. Osburn, "Detection of E.coli O157:H7 using a miniaturized surface plasmon resonance ... B. K. Oh, W. Lee, Y. K. Kim, W. H. Lee, and J. W. Choi, "Surface plasmon resonance immunosensor using self-assembled protein G ...
Accelerating Antibody Screening with Array-Based Surface Plasmon Resonance. * Broadcast Date: Wednesday, Jan 17, 2018 ... With the emergence of surface plasmon resonance (SPR) arrays, investigators now have high-throughput capabilities to accelerate ...
"Sensitivity comparison of surface plasmon resonance and plasmon-waveguide resonance biosensors," Sensors and Actuators, B: ... Plasmon resonance spectroscopy: probing molecular interactions at surfaces and interfaces. Zdzislaw Salamon and Gordon Tollin ... Erik Reimhult, Charlotte Larsson, Bengt Kasemo, and Fredrik Höök, "Simultaneous surface plasmon resonance and quartz crystal ... Matthew W. Meyer, Kristopher J. McKee, Vy H. T. Nguyen, and Emily A. Smith, "Scanning Angle Plasmon Waveguide Resonance Raman ...
Substantial LSPR peak shifts due to the adsorption of molecules layer on a NP surface were observed for nanoparticles with ~5 ... Moreover, this work provides insights on the LSPR behavior due to adsorption of molecular layer on a NP surface, establishing a ... we introduce a new perspective on the development of Localized Surface Plasmon Resonance (LSPR) optical biosensors. ... While for surface plasmon resonance thin film based sensors the evanescent field decay length is of the order 200 nm to 300 nm ...
Surface Plasmon Resonance imaging (SPRi) is a label-free optical detection technique used to monitor and analyze biomolecular ... Introduction into Surface Plasmon Resonance imaging (SPRi). Surface Plasmon Resonance imaging (SPRi) is a label-free optical ... Surface Science Techniques * Plasma Profiling Time-Of-Flight Mass Spectrometry (PP-TOFMS) ... SPRi is used to monitor changes of the refractive index occurring at the surface of the SPRi-Biochip. A binding event, or mass ...
Surface Plasmon Resonance imaging (SPRi) is a label-free optical detection technique used to monitor and analyze biomolecular ... Introduction into Surface Plasmon Resonance imaging (SPRi). Surface Plasmon Resonance imaging (SPRi) is a label-free optical ... Surface Science Techniques * back to By Technique Surface Science Techniques * Plasma Profiling Time-Of-Flight Mass ... Surface Science Techniques * back to Measurement and Control Techniques Surface Science Techniques ...
... allows identifying only those analytes that specifically interact with biologically active substance immobilized on the surface ... surface plasmon resonance (SPR) has become one of the major methods for studying and determination of biologically active ... surface plasmon resonance immunosensor; biomedical research; immunoassay; biosensor; surface plasmon resonance This is an open ... Surface plasmon resonance and its application to biomedical research by Asta Kaušaitė 1,2, Almira Ramanavičienė 1,2, Viktoras ...
... a Surface PlasmonResonance sensor for detecting n-dodecane vapor is developed. Preliminary results will bepresented, showing ... Development of a Surface Plasmon Resonance n-dodecane Vapor Sensor. Narcizo Muñoz Aguirre 1,* , Lilia Martínez Pérez 2. ... Aguirre, N.M.; Pérez, L.M.; Colín, J.A.; Buenrostro-Gonzalez, E. Development of a Surface Plasmon Resonance n-dodecane Vapor ... "Development of a Surface Plasmon Resonance n-dodecane Vapor Sensor." Sensors 7, no. 9: 1954-1961. ...
A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real-time as a function of ... Blood plasma coagulation studied by surface plasmon resonance Author(s): Trine P. Vikinge; Kenny M. Hansson; Johan Benesch; ... Atomic force microscopy pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network ...
Résonance de plasmons de surface (SPR) est le phénomène optique sous-jacente derrière exempte détiquette de biocapteurs pour ... Résonance de plasmons de surface (SPR) est le phénomène optique sous-jacente derrière exempte détiquette de biocapteurs pour ... surface plasmon résonance, ou SPR, est le phénomène sous-jacent derrière certains biocapteurs exempte détiquette pour ... Si nécessaire, la surface du capteur est amorcée avec un substrat pour capturer le ligand. Le ligand est coulé sur la surface ...
Liebsch A. Surface-plasmon dispersion and size dependence of Mie resonance: silver versus simple metals. Phys Rev B 1993;48: ... Blueshift of the surface plasmon resonance in silver nanoparticles: substrate effects. Søren Raza, Wei Yan, Nicolas Stenger, ... We study the surface plasmon (SP) resonance energy of isolated spherical Ag nanoparticles dispersed on a silicon nitride ... We have investigated the surface plasmon resonance of spherical silver nanoparticles ranging from 26 down to 3.5 nm in size ...
Sensitive surface plasmon resonance biosensor enhanced by photonic crystal metallic... Proceedings of SPIE (June 02 2014) ... Numerical analysis and comparison of surface plasmon resonance (SPR)-based photonic crystal fiber (PCF) structures are ... Ahmet Yasli, Huseyin Ademgil, "Geometrical comparison of photonic crystal fiber-based surface plasmon resonance sensors," ... Ahmet Yasli, Huseyin Ademgil, "Geometrical comparison of photonic crystal fiber-based surface plasmon resonance sensors," Opt. ...
Innovative Exploitation of Grating-Coupled Surface Plasmon Resonance for Sensing , IntechOpen, Published on: 2012-10-24. ... 7 - Homola J., Surface Plasmon Resonance Based Sensors.Springer, 2006. 8 - Karlsson R., Stahlberg R., Surface plasmon resonance ... Surface Plasmon Resonance for sensing: the deposition of the analyte on the SPP-supporting surface results in a change of the ... Figure 2. Surface Plasmon Resonance for sensing: the deposition of the analyte on the SPP-supporting surface results in a ...
Surface plasmon resonance (SPR) sensors proved themselves as a promising device for many kinds of applications such as optical ... Surface Plasmon Resonance Sensors: Optimization of Diffraction Grating and Prism Couplers. W. Raja[1], A. Alabastri[1], S. ... we illustrate a comprehensive designing rule to determine the parameters for optimizing the generation of surface plasmon ...
Local surface plasmon resonance mode (LSPR) mapping of gold pyramids using cathodoluminescence. (a) Composite image of ... Surface plasmon resonance modes. Local surface plasmon resonance mode (LSPR) mapping of gold pyramids using cathodoluminescence ...
... immobilized on the gold surface of a self-assembled surface plasmon resonance (SPR) apparatus. A kind of self-assembled ... Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on surface plasmon resonance.. Chou SF1, ...
We report a simple label-free localized surface plasmon resonance sensor that uses the multiple resonances of a U-shaped gold ...
... surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real ... Surface plasmon resonance (SPR) Antiresonant reflecting optical waveguide type B (ARROW-B) Biosensor Sensor regeneration Single ... Homola, J. (2008). Surface plasmon resonance sensors for detection of chemical and biological species. Chemical Reviews, 108, ... Surface plasmon resonance (SPR) biosensors have been extensively investigated due to their advantages, such as high sensitivity ...
Surface plasmon resonance biosensors Proceedings of SPIE (July 02 2007) Miniature fiber optic surface plasmon resonance ... Y. Chen and H. Ming, "Review of surface plasmon resonance and localized surface plasmon resonance sensor," Photonic Sens. 2(1 ... Surface plasmon resonance response of a polymer-coated biochemical sensor Proceedings of SPIE (February 09 1995) ... S. H. Kim et al., "The effect of Au/Ag bimetallic thin-films on surface plasmon resonance properties comparing with those of Au ...
... about SURFACE PLASMON RESONANCE. Search and download thousands of Swedish university dissertations. Full text. Free. ... surface plasmon resonance. Showing result 1 - 5 of 171 swedish dissertations containing the words surface plasmon resonance. ... Keywords : NATURAL SCIENCES; NATURVETENSKAP; Chemistry; Protein; Adsorption; Immobilization; Surface; Surface Plasmon Resonance ... Carbohydrate surface immobilization; Carbohydrate microarrays; Surface plasmon resonance SPR ; Quartz crystal microbalance QCM ...
J. Homola, Surface Plasmon Resonance Based Sensors (Springer, 2006) * W. Peng, Y. Liu, P. Fang, X. Liu, Z. Gong, H. Wang, and F ... Hollow fiber surface plasmon resonance sensor for the detection of liquid with high refractive index Bing-Hong Liu, Yong-Xiang ... A fibre optic surface plasmon resonance (SPR) sensor system for smartphones is reported, for the first time. The sensor was ... Compact multi-channel surface plasmon resonance sensor for real-time multi-analyte biosensing Yun Liu, Shimeng Chen, Qiang Liu ...
Surface plasmon resonance sensor based on a novel grapefruit photonic crystal fiber Author(s): Peipei Zhang; Jianquan Yao; Lei ... We propose a novel surface plasmon resonance sensor design based on a grapefruit photonic crystal fiber. In such a sensor, ... phase matching between plasmon and a core mode is achieved by introducing microstructure into the fiber core. Using the finite ...
Torrell M et al (2011) Tuning of the surface plasmon resonance in TiO2/Au thin films grown by magnetron sputtering: the effect ... Evolution of the surface plasmon resonance of Au:TiO2 nanocomposite thin films with annealing temperature. ... Thin films Au nanoparticles TiO2 matrix Nanocomposites Localized surface plasmon resonance Dielectric function ... Hutter E, Fendler JH (2004) Exploitation of localized surface plasmon resonance. Adv Mater 16:1685-1706. doi: 10.1002/adma. ...
  • Karlsson R and Fält A (1997) Experimental design for kinetic analysis of protein-protein interactions with surface plasmon resonance biosensors. (els.net)
  • Surface plasmon resonance (SPR) is the underlying optical phenomenon behind label-free biosensors to evaluate the molecular affinity, kinetics, specificity, and concentration of biomolecules. (jove.com)
  • Surface plasmon resonance, or SPR, is the underlying phenomenon behind certain label-free biosensors for evaluating binding and adsorption interactions of biomolecules. (jove.com)
  • In this work, we introduce a new perspective on the development of Localized Surface Plasmon Resonance (LSPR) optical biosensors. (scirp.org)
  • Using SPR biosensors, it is possible to analyze the mixtures of substances with a very similar chemical structure because SPR allows identifying only those analytes that specifically interact with biologically active substance immobilized on the surface of SPR biosensor. (mdpi.com)
  • The increasing variety of surface linkage chemistries broadens the utility of analytical biosensors for a wide range of applications. (bruker.com)
  • The surface plasmon resonance (SPR) phenomenon is utilized in a number of new real time biosensors. (diva-portal.org)
  • Plasmon-Enhanced Fluorescence Biosensors: a Review. (ebscohost.com)
  • In order to enable local functionalization of label-free optical waveguide biosensors in a cost effective mass-fabrication compatible manner, we investigate surface modification employing inkjet printing of functional polymers and UV-curable benzophenone dextran. (technologynetworks.com)
  • Reference : Surface plasmon resonance-based biosensors: From the development of different SPR str. (ac.be)
  • en] Surface plasmon resonance (SPR)-based biosensors are very powerful tools for the study of biomolecular interactions, chemical detection and immunoassays. (ac.be)
  • Some aspects of their surface functionalization, the key element which imparts biofunctionality to these structures and hence transforming them into biosensors, will also be discussed accordingly. (ac.be)
  • The ultimate performance of SPR-based biosensors will thus be determined by both their inherent optical performance and suitable surface functionalization. (ac.be)
  • DeGrasse, S.L. / An improved immunoassay for detection of saxitoxin by surface plasmon resonance biosensors . (qub.ac.uk)
  • The surface plasmon resonance has been utilized in many industries including food and beverages, pharmaceuticals, electronics for biosensors and healthcare for drug discovery. (industrystatsreport.com)
  • On the basis of application, the global surface plasmon resonance market is divided into cathode-ray oscilloscope (CRO), drug discovery biosensors, material science. (industrystatsreport.com)
  • SPR is the basis of many standard tools for measuring adsorption of material onto planar metal (typically gold or silver) surfaces or onto the surface of metal nanoparticles. (wikipedia.org)
  • LSPRs (localized surface plasmon resonances) are collective electron charge oscillations in metallic nanoparticles that are excited by light. (wikipedia.org)
  • SPRM is used to characterize surfaces such as self-assembled monolayers, multilayer films, metal nanoparticles, oligonucleotide arrays, and binding and reduction reactions. (wikipedia.org)
  • Another technique called localized, or 'L'SPR, uses metal nanoparticles as the sensor surface. (jove.com)
  • Substantial LSPR peak shifts due to the adsorption of molecules layer on a NP surface were observed for nanoparticles with ~5 nm and ~40 nm radius. (scirp.org)
  • Herein we demonstrate the sensitivity enhancement with Au nanoparticles coupled- SPR immuno-sensor chip on which the specific size and surface density of the particles controlled as a label-free detection system. (nsti.org)
  • Surface density of immobilized 30 nm Au nanoparticles on bare Au film was estimated as 1x109 ea/cm2 using atomic force microscopy. (nsti.org)
  • We study the surface plasmon (SP) resonance energy of isolated spherical Ag nanoparticles dispersed on a silicon nitride substrate in the diameter range 3.5-26 nm with monochromated electron energy-loss spectroscopy. (degruyter.com)
  • Localized surface plasmon resonances (LSPRs) in noble-metal nanoparticles possess very high refractive index sensitivity close to the metal surface and therefore constitute an attractive biosensing platform. (dissertations.se)
  • The interaction between incident light and surface electrons in conductive nanoparticles produces localized plasmon oscillations with a resonant frequency that strongly depends on the composition, size, geometry, and dielectric environment. (rsc.org)
  • Metal nanoparticles exhibit the phenomenon of localized surface plasmon resonance (LSPR) due to the collective oscillation of their conduction electrons, which is induced by external electromagnetic radiation. (ebscohost.com)
  • Localized surface plasmon resonances excited in metallic nanoparticles confine and enhance electromagnetic fields at the nanoscale. (ebscohost.com)
  • Surfaces of metallic films and metallic nanoparticles can strongly confine electromagnetic field through its coupling to propagating or localized surface plasmons. (ebscohost.com)
  • Wang H, Tong Q, Yan M. Antifouling surfaces for proteins labeled with dye-doped silica nanoparticles. (umassmed.edu)
  • Influence of surface plasmon resonances of silver nanoparticles on optical and electrical properties of textured silicon solar cell. (bentham.co.uk)
  • Here, we report average reflectance reduction of ∼8% in wavelength range of 300-1100 nm after coupling surface plasmon resonances (SPRs) of silver nanoparticles (NPs) to textured silicon (T-Si) surface. (bentham.co.uk)
  • Recent study shows that Plasmon Resonance (SPR) is widely used in the development of nanoparticles (NPs) for biomedical purposes, for enhancing the pharmacokinetic profile of drugs by targeting specific disease site. (industrystatsreport.com)
  • We present a new Surface Plasmon Resonance imager (SPRi) based on immobilized T4-phage for bacteria detection. (hindawi.com)
  • Surface Plasmon Resonance imaging (SPRi) is a label-free optical detection technique used to monitor and analyze biomolecular interactions in real time. (horiba.com)
  • Surface plasmon resonance (SPR) is an optical-based, label-free detection technology for real-time monitoring of binding interactions between two or more molecules. (bruker.com)
  • An immunosensor assay for the quantitation of intracellular recombinant human superoxide dismutase (rhSOD) in Escherichia coli cultivations based on detection with surface plasmon resoance (SPR) is described. (diva-portal.org)
  • Accordingly, a fast colorimetric assay within 5 min for the detection of prostate specific antigen (PSA) was developed based on a AuNC/GNRs heterostructure mask regulated by the target molecule under photon-plasmon coupling. (rsc.org)
  • Novel surface plasmon resonance sensor for the detection of heme at biological levels via highly selective recognition by apo-hemoglobin. (umassmed.edu)
  • By attaching an azide functional group via a tetraethylene glycol linker to the α-terminal position of a variety of oligothiophenes, thiophene-based ligands that can be utilized for detection of protein aggregates with surface plasmon resonance have been developed. (diva-portal.org)
  • By making asymmetrical LCOs, which can be attached to a surface, we also foresee a methodology that will offer the possibility to create a sensitive and selective detection method, and maybe lead to a lab-on-a-chip-application. (diva-portal.org)
  • Direct measurement of proteins in serum samples by SPR imaging was achieved by developing methods to minimize nonspecific adsorption onto the avidin-functionalized surface, and a limit of detection (LOD) of 6.7 nM IgG was obtained for the treated serum samples. (ebscohost.com)
  • We describe the positive effect of surface plasmon-coupled fluorescence emission (SPCE) on the detection of a signal from a surface immunoassay in highly absorbing or/and scattering samples. (ebscohost.com)
  • This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). (dtu.dk)
  • This paper reviews the performance of various SPR structures and detection schemes focusing on propagating surface plasmons generated in planar structures. (ac.be)
  • An optical sensor based on surface plasmon resonance phenomenon for detection of chlorine in pure water and swimming pool water is presented. (thescipub.com)
  • The shift of resonance angle (Î θ) increases linearly with chlorine concentration in which the detection limit and sensor sensitivity could be quantified. (thescipub.com)
  • The results show that the shift of resonance angle (Δθ SPP ) increases linearly with the sugar content in which the detection limit and sensor sensitivity could be quantified. (thescipub.com)
  • In this study, we performed finite element method (FEM) simulations to optimize the configuration of gold nanorods (GNR) enhanced surface plasmon resonance (SPR) sensor and discovered its application for multiplex antigens detection. (ntu.edu.sg)
  • Surface plasmon resonance (SPR) is a widely used label-free detection technique for studying binding interactions of biomolecules. (drugtargetreview.com)
  • Fabrication of a novel immunosensor using functionalized self-assembled monolayer for trace level detection of TNT by surface plasmon resonance. (semanticscholar.org)
  • We have developed a new immunosensor based on self-assembly chemistry for highly sensitive and label-free detection of 2,4,6-trinitrotoluene (TNT) using surface plasmon resonance (SPR). (semanticscholar.org)
  • The global surface plasmon resonance market is mainly driven by increasing adoption of label-free detection techniques over labelled detection techniques due to cost-effectiveness, availability of versatile products in the SPR market for refined outcomes and coupling of complementary techniques with SPR to enhance specificity of the test are major factors leading to increased demand for surface plasmon resonance. (mrrse.com)
  • Emergence of alternative techniques for detection of protein and high prices of products are few factors expected to hamper growth of the global surface plasmon resonance market to a certain extent. (mrrse.com)
  • Surface Plasmon Resonance imaging (SPRi) is an optical detection process that occurs when a polarized light hits a prism covered by a thin (gold) metal layer. (francelab.hu)
  • Lastly, the extreme phase sensitivity of graphene-protected copper is used to detect trace quantities of small toxins in solution far below the detection limit of commercial surface plasmon resonance sensors. (worldcat.org)
  • In this thesis, the structure and stability of globular proteins adsorbed onto nanometer-sized hydrophilic silica particles were investigated using differential scanning calorimetry (DSC), hydrogen/deuterium exchange (HDX), and mass spectrometry (MS). The adsorption process itself was characterized with fluorescence and absorption spectroscopy and surface plasmon resonance (SPR). (dissertations.se)
  • Surface plasmon resonance (SPR) spectroscopy is a powerful, label-free technique to monitor noncovalent molecular interactions in real time and in a noninvasive fashion. (ed.gov)
  • Surface plasmon resonance spectroscopy for characterisation of membrane protein-ligand interactions and its potential for drug discovery. (creative-biostructure.com)
  • Surface plasmon resonance imaging studies of protein-carbohydrate interactions. (nih.gov)
  • Carbohydrate arrays fabricated on gold films were used to study carbohydrate-protein interactions with surface plasmon resonance (SPR) imaging. (nih.gov)
  • SPR imaging measurements were employed to accomplish the following: (i) construct adsorption isotherms for the interactions of ConA and jacalin to the carbohydrate surfaces, (ii) monitor protein binding to surfaces presenting different compositions of the immobilized carbohydrates, and (iii) measure the solution equilibrium dissociation constants for ConA and jacalin toward mannose and galactose, respectively. (nih.gov)
  • Biomedical applications take advantage of the exquisite sensitivity of SPR to the refractive index of the medium next to the metal surface, which makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands. (hindawi.com)
  • Surface-plasmon resonance (SPR) sensing is a widely used optical technique for detecting and analyzing biochemical interactions. (uky.edu)
  • Students working on this project will study the interaction of low density monolayers with certain proteins, the functionalization of these monolayers to target specific interactions, and/or the further development of multi-mode surface-plasmon resonance sensor systems. (uky.edu)
  • In the recent years, surface plasmon resonance (SPR) has become one of the major methods for studying and determination of biologically active materials exhibiting affinity interactions. (mdpi.com)
  • Binding interactions between a molecule on the sensor surface (ligand) and its binding partner(s) in solution (analyte) are monitored in real-time by SPR. (bruker.com)
  • From initial screening to detailed kinetic characterization and thermodynamics measurements, the Sierra SPR-32 system enables high-throughput surface plasmon resonance (SPR) analysis of molecular interactions. (bruker.com)
  • An in vitro technique based on an optical phenomenon, called surface plasmon resonance (SPR), can simultaneously detect interactions between unmodified proteins and directly measure kinetic parameters of the interaction. (currentprotocols.com)
  • Surface plasmon resonance based methods for measuring the kinetics and binding affinities of biomolecular interactions. (currentprotocols.com)
  • Surface Plasmon Resonance (SPR) is a powerful technology to study macromolecular interactions, allowing direct and rapid determination of association and dissociation rates using small amounts of samples. (bio-protocol.org)
  • Surface plasmon resonance (SPR) has provided crucial information on the mechanisms of molecular interactions accompanying varied aspects of plant development. (omicsonline.org)
  • We report the multiplexed, simultaneous analysis of antigenâ€"antibody interactions that involve human immunoglobulin G (IgG) on a gold substrate by the surface plasmon resonance imaging method. (ebscohost.com)
  • A surface plasmon resonance (SPR) biosensor technology has recently been applied biochemically and clinically to the study of immunologic recognition and the evaluation of binding parameters for various interactions between antibodies (Abs) and antigens (Ags) at liquid-solid interface. (ebscohost.com)
  • In this study, we assess the binding interactions of flavonoids with human serum albumin (HSA), the most abundant protein in the blood, and with glutathione S transferase pi isoform-1 (GSTP-1), an enzyme with well characterized hydrophobic binding sites that plays an important role in detoxification of xenobiotics with reduced glutathione, using a novel Taylor dispersion surface plasmon resonance (SPR) technique. (arvojournals.org)
  • Surface plasmon resonance for real-time study of lectin-carbohydrate interactions for the differentiation and identification of glycoproteins. (lu.se)
  • A study of specific interactions between lectins and glycoproteins has been carried out using surface plasmon resonance (SPR) in a flow-injection mode. (lu.se)
  • Specific lectin-glycoprotein interactions caused the shift of refractive index proportional to the mass concentration accumulated on the channel surface. (lu.se)
  • In order to clarify the chemical bases for these interactions, glycosaminoglycan solutions were injected onto sensor surfaces on which collagens, fibronectin, laminin, and vitronectin were immobilized. (researchmap.jp)
  • XelPleX combines the power of multiplexing (measuring multiple interactions simultaneously) and the sensitivity of Surface Plasmon Resonance, resulting in highresolution kinetic profiles. (francelab.hu)
  • Surface plasmon resonance (SPR) allows examination of protein-protein interactions in real time, from which both binding affinities and kinetics can be directly determined. (elsevier.com)
  • Surface Plasmon Resonance instruments are used for studying biomolecular interactions and conformational changes in real time. (spectraresearch.com)
  • Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information. (springer.com)
  • This allows the development of optical sensors designed to detect/differentiate between samples exhibiting different refractive index, or monitor biomolecule attachment onto the transducer surface [ 14 ]. (hindawi.com)
  • Dr. Berron's group has developed stable, low-density self-assembled monolayers that are ideal for functionalizing the gold surfaces common to SPR sensors. (uky.edu)
  • Ahmet Yasli, Huseyin Ademgil, "Geometrical comparison of photonic crystal fiber-based surface plasmon resonance sensors," Optical Engineering 57(3), 030801 (5 March 2018). (spiedigitallibrary.org)
  • Surface plasmon resonance (SPR) sensors proved themselves as a promising device for many kinds of applications such as optical biosensing, binding constant determinationor nanofilm thickness measurements. (comsol.com)
  • Immobilization of proteins to a carboxymethyldextran‐modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. (currentprotocols.com)
  • First surface-coating development was performed for both sensors and enzyme reactors. (uu.nl)
  • Surface plasmon resonance microscopy (SPRM), also called surface plasmon resonance imaging (SPRI), is a label free analytical tool that combines the surface plasmon resonance of metallic surfaces with imaging of the metallic surface. (wikipedia.org)
  • Our technology measures modifications of the refractive index at the surface of the SPRi-Biochip, which can be correlated to mass variations. (horiba.com)
  • SPRi is used to monitor changes of the refractive index occurring at the surface of the SPRi-Biochip. (horiba.com)
  • The ligands are immobilized in an array format on the functionalized SPRi-Biochip surface. (horiba.com)
  • Surface plasmon resonance imaging (SPRi) provides a label-free, multiplex analytical platform for pathogen screening. (springer.com)
  • Herein we have combined an underutilized fragment screening method, native state mass spectrometry, together with two proven and popular fragment screening methods, surface plasmon resonance and X-ray crystallography, in a fragment screening campaign against human carbonic anhydrase II (CA II). (rcsb.org)
  • The interaction between the bovine prion protein (bPrP) and a monoclonal antibody, 1E5, was studied with high-mass matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) and surface plasmon resonance (SPR). (uzh.ch)
  • The performance of (1) nanoelectrospray ionization mass spectrometry (nESI-MS), (2) surface plasmon resonance (SPR), and (3) isothermal titration calorimetry (ITC) was compared for the determination of dissociation constants (K(D)). The model system studied for this purpose was the human carbonic anhydrase I (hCAI) with eight known and well characterized sulfonamide inhibitors (Krishnamurthy et al. (uzh.ch)
  • The heterogeneity of the refractive index of the metallic surface imparts high contrast images, caused by the shift in the resonance angle. (wikipedia.org)
  • A variation of the refractive index of the dielectric interface induces a variation of the resonance condition. (hindawi.com)
  • The local changes at the surface of the chip recorded as changes of refractive index provide detailed information on any interaction of kinetic process taking place. (hindawi.com)
  • A binding event, or mass accumulation, will induce a change of refractive index and a shift of the position of the resonance angle. (horiba.com)
  • b) Resonance angle shift as a function of refractive index variation of water solution with increasing NaCl concentration. (intechopen.com)
  • As molecules bind, the refractive index close to the surface changes causing a shift in the angle of minimum reflected intenstiy. (bruker.com)
  • The refractive index of light reflecting off this surface is measured. (umassmed.edu)
  • From the plasmon band shift versus the refractive index, ultra-high local surface plasmon resonance sensitivity (413 nm RIU-1 or 1.24 eV RIU-1, figure of merit (FOM) = 4.59) was reached at similar to 630 nm, making these materials promising for chemical/biological sensing applications. (uva.nl)
  • Figure 1.The changes in the refractive index in the immediate vicinity of the surface layer of a sensor chip can be detected by SPR. (creative-biostructure.com)
  • When analyte molecules bind to the surface, the critical angle that is dependent on the refractive index will change. (creative-biostructure.com)
  • When the analyte (e.g., an antigen) is introduced, two things happen: there is a change in refractive index due to buffer mismatch, and a secondary, much smaller change due to binding of analyte to ligand (e.g., its antibody, covalently coupled) on the surface. (biosensorcore.com)
  • This technique is simply based on the optical property of light and principally measures the change in the refractive index upon binding of any molecule to the surface. (bio-protocol.org)
  • This manuscript describes a more rigorous and stable SPR inhibition immunoassay through optimization of the surface chemistry as well as determination of optimum mixture ratios and mixing times. (qub.ac.uk)
  • Para-maleimidophenyl (p-MP) modified gold surfaces have been prepared by one-step electrochemical deposition and used in surface plasmon resonance (SPR) studies. (fraunhofer.de)
  • In the case of p-polarized light (polarization occurs parallel to the plane of incidence), this is possible by passing the light through a block of glass to increase the wavenumber (and the momentum), and achieve the resonance at a given wavelength and angle. (wikipedia.org)
  • Surface plasmon resonance is an optical resonance effect where the backside of a thin conductive mirror affects the angle at which there is a minimum of reflected light. (els.net)
  • Simultaneously, the equipment monitors the changes in light reflectivity over the whole surface at a fixed resonance angle and in real time. (hindawi.com)
  • Surface plasmon resonance (SPR) is a phenomenon occuring at metal surfaces (typically gold and silver) when an incident light beam strikes the surface at a particular angle. (hindawi.com)
  • As molecules bind to the surface, the electronic properties of the metal change, which in turn adjusts the angle. (jove.com)
  • With systematic control of the size of the Au nanoparticle and thickness of the bare Au film, it was found that 30 nm Au particles on 50 nm thick Au film demonstrated the largest resonance angle shift for surface reaction on the chip. (nsti.org)
  • In the inset graph: resonance angle θres as a function of the azimuth angle φ: experimental points and simulation curve (solid line). (intechopen.com)
  • Resonance angle shift as a function of azimuth angle for sodium-chloride concentration: 0.56g/200 ml(water), Δn = 5.4 10-4. (intechopen.com)
  • Angle-scanning SPR has been used to study oligonucleotide hybridization to surface confined probes, and work is underway to apply SPR imaging to study DNA hybridization in macro- and microarray formats. (spie.org)
  • SPR uses a type of total internal reflection and causes a reduction in the light intensity reflected at a specific angle from the glass side of the sensor surface. (bruker.com)
  • The specific interaction between target molecules and Au nanoparticleantibody conjugate induce the change of the surface properties such as mass and roughness, which can be represented as the change of plasmon resonance angle. (nsti.org)
  • When the air medium outside the metal film is changed to chlorine solution, the resonance angle shifted to the higher value. (thescipub.com)
  • We observed that the shift in the resonance angle (Πθ) decreases with time due to reducing amount of chlorine in the solution. (thescipub.com)
  • Both have shown that the resonance angle decreases with time due to releasing of chlorine gas to the atmosphere. (thescipub.com)
  • The angle of resonance is sensitive to both the experimental configuration used and to the dielectric constant of the material adjacent to the metal surface. (comsol.co.in)
  • Light source launches the polarised light that directs to the under surface of the gold film and there are surface plasmons generated at a critical angle. (creative-biostructure.com)
  • The light is directed through a prism to the surface of the foil at a fixed angle that is consistent with Total Internal Reflection . (biosensorcore.com)
  • The binding of 1 pg of material to the chip surface in the microhydraulic chamber gives a change in the position of the shadow that causes 1/10000th of a degree of shift in the angle of reflection, and this is read out as 1 Response Unit, or RU. (biosensorcore.com)
  • Under certain conditions (wavelength, polarization and incidence angle), free electrons at the surface of the biochip absorb incident light photons and convert them into surface plasmon waves. (francelab.hu)
  • The resonance occurs when the free electrons of the metal oscillate (plasmon wave) and absorb the plane-polarized light at the specific angle (SPR angle). (bio-protocol.org)
  • At the SPR angle, a sharp decrease in the intensity of the reflected light (since some of light is transferred to the Plasmon wave) is observed and this serves as the measurement key. (bio-protocol.org)
  • For example, when proteins bind to ligands attached (through a linker) to the gold surface, a change in dielectric constant occurs which is correlated with SPR change. (els.net)
  • After enzymatic C3b coupling or standard amine C3b coupling, we analyzed and compared the binding of four C3b ligands to the surface: factor B, factor H, C5 and the soluble complement receptor 1 (sCR1, CD35). (diva-portal.org)
  • When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. (umassmed.edu)
  • All ligands displayed selectivity towards recombinant amyloid fibrils and the LCO/protein aggregate interaction could be detected by fluorescence as well as by surface plasmon resonance. (diva-portal.org)
  • Finally, a method for quantifying binding of proteins and other ligands to the surfaces of phospholipid bilayered vesicles (attached intact to the SPR surface via the SA layer) is demonstrated, and applied to determine the dissociation equilibrium constant for phospholipase A2 binding to membranes. (washington.edu)
  • Carotenoids as possible interphotoreceptor retinoid-binding protein (IRBP) ligands: a surface plasmon resonance (SPR) based study. (semanticscholar.org)
  • Since the wave is on the boundary of the conductor and the external medium (air, water or vacuum for example), these oscillations are very sensitive to any change of this boundary, such as the adsorption of molecules to the conducting surface. (wikipedia.org)
  • Langmuir I (1918) The adsorption of gases on plane surfaces of glass, mica and platinum. (els.net)
  • Adsorption coefficients (K(ADS)) of 2.2 +/- 0.8 x 10(7) M(-)(1) and 5.6 +/- 1.7 x 10(6) M(-)(1) were obtained for jacalin adsorbing to a galactose surface and ConA adsorbing to a mannose surface, respectively. (nih.gov)
  • Moreover, this work provides insights on the LSPR behavior due to adsorption of molecular layer on a NP surface, establishing a new paradigm on engineering LSPR biosensor. (scirp.org)
  • SPR technology is based on the electromagnetic field component of incident light penetrating into a surface and it can be used to detect molecular adsorption on surfaces. (comsol.fr)
  • By modifying the sensing surface with a proper terminal receptor group, SPR provides a surface analytical tool for studying the kinetics of surface adsorption and desorption reactions in situ. (washington.edu)
  • Next, a method for estimating sticking probabilities (rate per collision) and intrinsic rate constants for adsorption from liquid solutions is described, where a simple numerical solution to Fick's Law is used to calculate the adsorbate concentrations at incremental distances from the surface as adsorption occurs. (washington.edu)
  • The resulting concentration nearest the surface is needed to calculate both the intrinsic rate constant for adsorption and the collision frequency of the adsorbate with the surface versus time or coverage. (washington.edu)
  • These methods are applied to alkylthiol adsorption on the Au SPR surface and streptavidin (SA) adsorption on mixed biotin-containing alkylthiolate monolayers. (washington.edu)
  • Among these are non-specific adsorption (NSA) and surface modification allowing protein coupling. (uu.nl)
  • SPR is a surface sensitive technique, which can be used to study hybridization in situ, and the use of colloidal metal tags provides excellent sensitivity. (spie.org)
  • Surface plasmon resonance technique offers benefits such as high specificity and sensitivity and is based on the principle of total internal reflection and is mainly used in pharmaceutical and biotechnology companies, food industry and clinical research organization. (mrrse.com)
  • In particular, metallic nanostructures capable of supporting surface plasmons can be designed to possess spectrally narrow plasmon resonances, which are of particular interest due to their exceptional sensitivity to their local environment. (worldcat.org)
  • However, the global surface plasmon resonance market is hampered by the high cost and investment for establishing new sensor technology and lack of sensitivity range. (industrystatsreport.com)
  • With the emergence of surface plasmon resonance (SPR) arrays, investigators now have high-throughput capabilities to accelerate their biotherapeutic mAb-screening campaigns, while using minimal volumes of samples that are often available only at low concentration and in limiting quantities. (genengnews.com)
  • The binding properties of these aptamers, including equilibrium and kinetic rate constants, were determined through a rapid, high-throughput approach using surface plasmon resonance (SPR) analysis to measure real-time binding. (caltech.edu)
  • To describe the existence and properties of surface plasmon polaritons, one can choose from various models (quantum theory, Drude model, etc. (wikipedia.org)
  • In order to excite surface plasmon polaritons in a resonant manner, one can use electron bombardment or incident light beam (visible and infrared are typical). (wikipedia.org)
  • Surface plasmon polaritons are surface electromagnetic waves coupled to oscillating free electrons of a metallic surface that propagate along a metal/dielectric interface. (wikipedia.org)
  • Surface plasmons or surface plasmon polaritons are generated by coupling of electrical field with free electrons in a metal. (wikipedia.org)
  • Radiation of different wavelengths (green, red and blue) was converted into surface plasmon polaritons, through the interaction of the photons at the metal/dielectric interface. (wikipedia.org)
  • In part c) of Figure 4, the intensity of the surface plasmon polaritons with the distance is shown. (wikipedia.org)
  • Welford K (1991) Surface plasmon-polaritons and their uses. (els.net)
  • Furthermore, we illustrate a comprehensive designing rule to determine the parameters for optimizing the generation of surface plasmon polaritons (SPP) for the both setups i.e. grating couplers and Kretschmann configuration. (comsol.com)
  • Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. (umassmed.edu)
  • In SPRM technique, plasmon surface polariton (PSP) waves are used for illumination. (wikipedia.org)
  • The heart of the SPR technique is to construct a dedicated surface for an assay. (springer.com)
  • We attempted to evaluate the affinity of the anionic phospholipids to cytochrome c by means of surface plasmon resonance (SPR) technique and to correlate it with the cytochrome c active site alterations and peroxidase activity. (sigmaaldrich.com)
  • The SPR technique is particularly suitable for analysis of the alternative complement pathway (AP) because the inherent nature of the latter is to amplify deposition of C3b on various surfaces. (diva-portal.org)
  • Characterisation of ultrathin Prussian Blue films has been performed using in situ electrochemical surface plasmon resonance technique. (ad-astra.ro)
  • Protein-Flavonoid Binding Studies Using a Novel Taylor Dispersion Surface Plasmon Resonance (SPR) Technique. (arvojournals.org)
  • The measurement was carried out at room temperature using Kretschmann surface plasmon resonance technique. (thescipub.com)
  • Surface plasmon resonance is a technique which is used to analyze kinetics of interaction between biomolecules. (mrrse.com)
  • Major trends in the surface plasmon resonance market include increased adoption of microfluidics technique to lower consumption of reagent, introduction of advanced imaging software for analysis of surface plasmon resonance and consistent investment in research and development by various companies. (mrrse.com)
  • Moreover, unlike ITC, this technique is not solution based and uses solid surface for ligand immobilization. (bio-protocol.org)
  • This in turn is fuelling growth of the global surface plasmon resonance market. (mrrse.com)
  • By region, the global surface plasmon resonance market has been segmented into North America, Latin America, Western Europe, Eastern Europe, Asia Pacific Excluding Japan (APEJ), Japan and Middle East & Africa (MEA). (mrrse.com)
  • The global surface plasmon resonance market is segmented on the product type, application, end-user and region and country level. (industrystatsreport.com)
  • On the basis of end-user, the global surface plasmon resonance market is bifurcated into Pharmaceutical & biopharmaceutical companies, food and beverage industry and academic and research institutes. (industrystatsreport.com)
  • The regions covered in global surface plasmon resonance market report are North America, Europe, Asia-Pacific and Rest of the World. (industrystatsreport.com)
  • The key factor for growth of global surface plasmon resonance market is increasing demand of the latest technology for R& D in the pharmaceutical and healthcare industry due to rising prevalence of chronic ailments like cancer, diabetes etc. (industrystatsreport.com)
  • Moreover rising technological advancement for extensive research & development of pharmaceutical drugs with the advent of nanomaterials as well as surge in government funding and initiatives may create huge opportunity for global surface plasmon resonance market in the forecast period. (industrystatsreport.com)
  • Hundreds of spots can be immobilized on the Biochip and their corresponding sensorgrams analyzed in parallel, generating access to multiplex information, thanks to Surface Plasmon Resonance imaging . (horiba.com)
  • Our trainers are experts in Surface Plasmon Resonance imaging. (horiba.com)
  • The central theme of this thesis is the use of imaging Surface Plasmon Resonance (iSPR) as a tool in the characterization of surfaces with laterally varying properties. (dissertations.se)
  • Surface Plasmon Resonance Imaging in Shirley is a company that specializes in Abrasive Products. (salespider.com)
  • On the basis on product type, the surface plasmon resonance market is segmented into sensor system, reagents, imaging system and others. (industrystatsreport.com)
  • Local surface plasmon resonance mode (LSPR) mapping of gold pyramids using cathodoluminescence. (gatan.com)
  • Hybrid gold nanorods and gold nanoclusters (GNR/AuNCs) heterostructures prepared by intimate integration of GNRs with AuNCs exhibit both localized surface plasmon resonance (LSPR) property and peroxidase-like activity. (rsc.org)
  • It is found that the catalytic activity of the AuNC/GNR heterostructure could be remarkably enhanced by LSPR induced by photon-plasmon coupling in the visible to near-infrared (NIR) region. (rsc.org)
  • Our unique nano-structured sensor surface uses localized SPR (LSPR) to deliver repeatable, highly sensitive kinetic data. (nicoyalife.com)
  • The surface plasmon polariton is a non-radiative electromagnetic surface wave that propagates in a direction parallel to the negative permittivity/dielectric material interface. (wikipedia.org)
  • Xiangang Luo and Teruya Ishihara, "Subwavelength photolithography based on surface-plasmon polariton resonance," Opt. (osapublishing.org)
  • The use of surface-plasmon polariton (SPP) resonance in the optical near field of a metallic mask to produce fine patterns with a resolution of subwavelength scale is proposed. (osapublishing.org)
  • DNA microarrays, which can contain thousands of discrete DNA sequences on a single surface, have become widely used for hybridization studies. (spie.org)
  • These approaches have been incorporated into a number of state-of-the-art nanobio-platforms, including carbohydrate microarrays, surface plasmon resonance (SPR), quartz crystal microbalance (QCM), atomic force microscopy (AFM), and glyconanomaterials. (dissertations.se)
  • This enhancement may result from both the effective surface area increase and the amplification of the resonance resonance effect. (nsti.org)
  • In the quasistatic limit, i.e., when the wavelength of the exciting electromagnetic wave considerably exceeds the dimensions of the structure, the local-response Drude theory predicts that the resonance energy of localized SPs is independent of the size of the nanostructure [4], and that the field enhancement created in the gap between two metallic nanostructures diverges for vanishing gap size [5]. (degruyter.com)
  • In this study, gold (Au) nanoparticle-antibody conjugate was applied for the signal enhancement of surface plasmon resonance (SPR). (nsti.org)
  • Using these monodisperse high-quality TSNPRs as building blocks, self-assembled TSNPRs consisting of six-tip based "hot spots" were realized for the first time as demonstrated in a high enhancement (similar to 10(7)) of surface-enhanced Raman scattering (SERS). (uva.nl)
  • Analyte-ligand association and dissociation events are monitored by changes in molecular mass accumulation at the solid-liquid interface on the sensor chip surface. (mcgill.ca)
  • When all the ligand-analyte complexes are fully dissociated (sometimes using a regeneration solution), the plasmon curves and the kinetic curves return to the initial state. (horiba.com)
  • Surface Plasmon Resonance for sensing: the deposition of the analyte on the SPP-supporting surface results in a change of the dispersion curve and a consequent shift of the resonance dip. (intechopen.com)
  • A multichannel, microfluidic chip was fabricated from poly(dimethylsiloxane) (PDMS) to selectively functionalize the surface and deliver the analyte solutions. (ebscohost.com)
  • In an experiment to detect the binding of molecules, the receptor molecule is usually immobilised on the sensor surface and the analyte molecules are place into the flow channel. (creative-biostructure.com)
  • Using COMSOL Multiphysics, the effect of the SPR on the electromagnetic field can be defined, which has allowed for the measurement of surface contaminants and nano-scale photonic devices. (comsol.fr)
  • Surface Plasmon is a plane-polarized electromagnetic wave that travels on the surface at the interface of the metal coating of the sensor disk (gold disk) and the dielectric medium (sample layer/buffer). (bio-protocol.org)
  • Winuprasith T, Suphantharika M, McClements DJ, He L. Spectroscopic studies of conformational changes of ß-lactoglobulin adsorbed on gold nanoparticle surfaces. (umassmed.edu)
  • The prepared surface characterization was implemented by atomic force microscopy (AFM, Autoprobe CP, PSI Inc., USA). (nsti.org)
  • For applications including proteomics, drug discovery, immunogenicity, and food analysis, state-of-the-art Surface Plasmon Resonance (SPR) technology allows for label-free, real-time biomolecular interaction analysis. (mcgill.ca)
  • In Surface Plasmon Resonance: Methods and Protocols , experts in the field present a wide variety of applications involving commercially available SPR instruments. (springer.com)
  • Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world. (springer.com)
  • A fluorescence microscope using a Surface plasmon resonance (SPR) includes a monochromatic light provision unit, an SPR sensor, and a first light detector. (google.com)
  • The SPR sensor excites surface plasmons to amplify a fluorescence signal of the specific fluorophore. (google.com)
  • 1] The present invention relates to a fluorescence microscope using Surface plasmon resonance (SPR), for maximizing a fluorescence signal of a bio-sample to which a flu- orophore is labeled, using SPR to enable monitoring of the bio-sample. (google.com)
  • Red blood cells do not attenuate the SPCE fluorescence in surface assays. (ebscohost.com)
  • To understand these contacts one can employ various experimental methods like Dynamic Light Scattering (DLS), Fluorescence Resonance Energy Transfer (FRET), Isothermal titration calorimetry (ITC), Chemical crosslinking, Co-immunoprecipitation (Co-IP), Surface Plasmon Resonance (SPR) and many more. (bio-protocol.org)
  • 1992. Biospecific interaction analysis using surface plasmon resonance applied to kinetic, binding site and concentration analysis, J. Chromat . (springer.com)
  • The sensing interface was constructed using avidin as a linker layer between the surface-bound biotinylated bovine serum albumin and biotinylated anti-human IgG antibodies. (ebscohost.com)
  • Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector. (currentprotocols.com)
  • These PCR products could be detected using a BIAcore 2000 surface plasmon resonance device using peptide nucleic acid as a sensor probe. (nih.gov)
  • This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). (bio-protocol.org)
  • Now the widely used SPR technologies, such as Biacore technology, use a prism to focus the light, the next-generation SPR instruments are also developed which use a surface based on nano-structured materials (Figure 2). (creative-biostructure.com)
  • They are created by incoming light striking a compatible surface, such as the gold foil used in the Biacore ® chip . (biosensorcore.com)
  • NB: Biacore ® instruments use the " Kretschman configuration " for Surface Plasmon Resonance (SPR), which places the prism very close to the foil when the chip is mounted in the instrument). (biosensorcore.com)
  • For example, the dextran chains linked to the Biacore ® CM5 chip surface are ~100 nm in length, and they are fully accessible over most of their lengths to be coupled to ligand because the gel-like environment they create (~2% hydrogel) is very porous. (biosensorcore.com)
  • A variety of sensor chip surfaces (eg. (mcgill.ca)
  • After each ligand injection the sensor chip surface is washed with running buffer (already set up in the pH scouting function). (bio-protocol.org)
  • This regeneration step is performed to completely remove the ligand from the sensor chip surface. (bio-protocol.org)
  • The positively charged ligand is electrostatically coupled to the negatively charged surface of the sensor chip, leading to ligand concentration. (bio-protocol.org)
  • Lectins were covalently immobilised on the surfaces of the microfluidic sensor chip via amine coupling and serum glycoproteins were injected into the flow channels. (lu.se)
  • Regeneration conditions for the surface of the sensor chip were found and optimised. (lu.se)
  • As shown in the figure, the surface of the basic instrument is a sensor chip which is a thin film of gold on a glass support. (creative-biostructure.com)
  • Depending on the thickness of a molecular layer at the metal surface, the SPR phenomenon results in a graded reduction in intensity of the reflected light. (hindawi.com)
  • Surface plasmon resonance biosensor based on Hg/Agâ€"Au film. (ebscohost.com)
  • The surface of the metallic mask on the illuminated side collects light through SPP coupling, and the interference of SPPs on the exit side of the metallic mask results in enhanced optical intensity with high spatial resolution, which can facilitate nanolithography efficiently by use of conventional photoresist with simple visible or ultraviolet light sources. (osapublishing.org)
  • Surface plasmon resonance is the optical instrument used to measure the effect of the binding molecules in real time. (industrystatsreport.com)
  • The North America surface plasmon resonance market was estimated to be valued at nearly US$ 250 Mn in 2017 and is forecasted to reach a valuation of nearly US$ 470 Mn in 2017, exhibiting a CAGR of 6.7% during the period of assessment from 2017-2027. (mrrse.com)
  • Antibody was immobilized with an oriented configuration on Au surface. (nsti.org)
  • Determination of Binding Constant and Stoichiometry for Antibody-Antigen Interaction with Surface Plasmon Resonance. (ebscohost.com)
  • However, HIV-1 uses several strategies to escape antibody neutralization, including mutation of the gp160 viral surface spike, a glycan shield to block antibody access to the spike, and expression of a limited number of viral surface spikes, which interferes with bivalent antibody binding. (pnas.org)
  • In order for the terms that describe the electronic surface plasmon to exist, the real part of the dielectric constant of the conductor must be negative and its magnitude must be greater than that of the dielectric. (wikipedia.org)
  • This field is highly localized at the nanoparticle and decays rapidly away from the nanoparticle/dieletric interface into the dielectric background, though far-field scattering by the particle is also enhanced by the resonance. (wikipedia.org)
  • Surface plasmons are collective excitations of the electron gas in metallic structures at the metal/dielectric interface [1]. (degruyter.com)
  • Surface Plasmon Resonance (SPR) is created by surface plasmons, coherent electron oscillations existing between any two materials where the real part of the dielectric function changes sign across the interface. (comsol.fr)
  • Strong coupling between diffraction-coupled plasmon resonances and a gold nanoparticle array and guided modes in a dielectric slab is used to construct a hybrid waveguide. (worldcat.org)
  • Simultaneously, this light generates an evanescent field, with its maximum intensity at the surface of the dielectric material. (bio-protocol.org)
  • Interestingly, CtxB and EtxB contain an extensive conserved segment spanning residues 45 to 74 that contains an exposed loop from Val-52 to Ile-58 located on the lower convoluted surface of the molecule ( 1 , 4 ). (pnas.org)
  • Lopez R, Haynes TE, Boatner LA, Feldman LC and Haglund RF Jr (2002) Temperature‐controlled surface plasmon resonance in VO 2 nanorods. (els.net)
  • Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on surface plasmon resonance. (nih.gov)
  • A direct human ferritin immunosensor was developed using anti-human ferritin monoclonal antibodies (MAbs) immobilized on the gold surface of a self-assembled surface plasmon resonance (SPR) apparatus. (nih.gov)
  • In this study, we selected a displacement method using a surface plasmon resonance (SPR) immunosensor, which is one of the ways to overcome these problems. (elsevier.com)
  • A novel surface plasmon resonance ( SPR ) biosensor which is capable of monitoring proteomic biomarker secretion from living cells is reported here. (rsc.org)
  • Protocols describe such surfaces for many needs, including the study of membrane bound proteins. (springer.com)
  • 1996. Optimum binding of anti-hygromycin B immunoglobulins with immobilized proteins A and G using a surface plasmon resonance biosensor. (springer.com)
  • The gold surface, which is required for SPR, is coated with a self-assembled monolayer to block nonspecific binding of proteins and to facilitate the attachment of molecules to the surface. (bruker.com)
  • The human serologic response to HIV-1 is polyclonal and targets both internal and viral surface proteins, but only antibodies directed against the HIV envelope spike, gp160, mediate viral neutralization ( 1 , 2 ). (pnas.org)
  • Another possibility is that high affinity binding of the B subunits to GM1 permits their direct lower affinity interaction with cell-surface signaling proteins. (pnas.org)
  • Numerical analysis and comparison of surface plasmon resonance (SPR)-based photonic crystal fiber (PCF) structures are presented. (spiedigitallibrary.org)
  • Figure 3 shows a schematic representation of surface plasmons coupled to electron density oscillations. (wikipedia.org)
  • Sample delivery to the sensor surface via a continuous flow microfluidic device yields the most accurate binding measurements. (bruker.com)
  • The sensor surface is often first coated with a substrate that has a high affinity for the metal. (jove.com)
  • Evaluation of cytochrome c affinity to anionic phospholipids by means of surface plasmon resonance. (sigmaaldrich.com)
  • Surface plasmon resonance (SPR) provides convenient real-time measurement of the reaction that enables subsequent estimation of the reaction constants necessary to determine binding affinity. (warwick.ac.uk)
  • Surface plasmon resonance (SPR) is the resonant oscillation of conduction electrons at the interface between negative and positive permittivity material stimulated by incident light. (wikipedia.org)
  • Sensitive surface plasmon resonance biosensor enhanced by photonic crystal metallic. (spiedigitallibrary.org)
  • The same effect of momentum-supply can be achieved by corrugating the metallic surface in the so-called Grating-Coupled SPR (c). (intechopen.com)
  • Using the plasmon hybridization method the authors examine light-induced coupling between the localized plasmons of a metallic nanosphere and the propagating plasmons of an infinite metallic wire. (ebscohost.com)
  • Thus, anything introduced into the medium that binds to the sensor's metallic surface can alter the SPR signal. (biosensorcore.com)
  • These waves are created by continuous fluctuation of charge at the metallic surface. (bio-protocol.org)
  • Highly selective rational design of peptide-based surface plasmon resonance sensor for direct determination of 2,4,6-trinitrotoluene (TNT) explosive. (nii.ac.jp)
  • This induces a shift of the plasmon curves and an increase of reflectivity. (horiba.com)
  • Light passes through a prism and reaches the surface (gold surface) where the molecules are bound. (bio-protocol.org)
  • The wave vector parallel to the surface is of sole importance in an SPR experiment since the plasmons are confined to the plane of the gold surface. (bio-protocol.org)
  • An immobilization scheme consisting of the formation of a surface disulfide bond was used to attach thiol-modified carbohydrates onto gold films and to fabricate carbohydrate arrays. (nih.gov)
  • The smaller circles represent equi-magnitude kSPP vectors before (a, c), and after (b, d) surface functionalization for the first (a, b) and the second (c, d) SPP excitation. (intechopen.com)