A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 22.214.171.124.
Protein D-Aspartate-L-Isoaspartate Methyltransferase
A generalized seizure disorder characterized by recurrent major motor seizures. The initial brief tonic phase is marked by trunk flexion followed by diffuse extension of the trunk and extremities. The clonic phase features rhythmic flexor contractions of the trunk and limbs, pupillary dilation, elevations of blood pressure and pulse, urinary incontinence, and tongue biting. This is followed by a profound state of depressed consciousness (post-ictal state) which gradually improves over minutes to hours. The disorder may be cryptogenic, familial, or symptomatic (caused by an identified disease process). (From Adams et al., Principles of Neurology, 6th ed, p329)
A childhood seizure disorder characterized by rhythmic electrical brain discharges of generalized onset. Clinical features include a sudden cessation of ongoing activity usually without loss of postural tone. Rhythmic blinking of the eyelids or lip smacking frequently accompanies the SEIZURES. The usual duration is 5-10 seconds, and multiple episodes may occur daily. Juvenile absence epilepsy is characterized by the juvenile onset of absence seizures and an increased incidence of myoclonus and tonic-clonic seizures. (Menkes, Textbook of Child Neurology, 5th ed, p736)
Area of the FRONTAL LOBE concerned with primary motor control located in the dorsal PRECENTRAL GYRUS immediately anterior to the central sulcus. It is comprised of three areas: the primary motor cortex located on the anterior paracentral lobule on the medial surface of the brain; the premotor cortex located anterior to the primary motor cortex; and the supplementary motor area located on the midline surface of the hemisphere anterior to the primary motor cortex.
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
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Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor. (1/976)Cripto-1 (CR-1) is a recently discovered protein of the epidermal growth factor family that fails to directly bind to any of the four known erb B type 1 receptor tyrosine kinases. The present study demonstrates that CR-1 indirectly induces tyrosine phosphorylation of erb B-4 but not of the epidermal growth factor-related receptors erb B-2 and erb B-3 in different mouse and human mammary epithelial cell lines. In addition, down-regulation of erb B-4 in NMuMG mouse mammary epithelial cells and in T47D human breast cancer cells, using an anti-erb B-4 blocking antibody or a hammerhead ribozyme vector targeted to erb B-4 mRNA, impairs the ability of CR-1 to fully activate mitogen-activated protein kinase. Finally, chemical cross-linking of 125I-CR-1 to mouse and human mammary epithelial cell membranes results in the labeling of two specific bands with a molecular weight of 130 and 60 kDa, suggesting that the CR-1 receptor represents a novel receptor structurally unrelated to any of the known type I receptor tyrosine kinases. In conclusion, these data demonstrate that CR-1, upon binding to an unknown receptor, can enhance the tyrosine kinase activity of erb B-4 and that a functional erb B-4 receptor is required for CR-1-induced MAPK activation. (+info)
Sortilin/neurotensin receptor-3 binds and mediates degradation of lipoprotein lipase. (2/976)Lipoprotein lipase and the receptor-associated protein (RAP) bind to overlapping sites on the low density lipoprotein receptor-related protein/alpha2-macroglobulin receptor (LRP). We have investigated if lipoprotein lipase interacts with the RAP binding but structurally distinct receptor sortilin/neurotensin receptor-3. We show, by chemical cross-linking and surface plasmon resonance analysis, that soluble sortilin binds lipoprotein lipase with an affinity similar to that of LRP. The binding was inhibited by heparin and RAP and by the newly discovered sortilin ligand neurotensin. In 35S-labeled 3T3-L1 adipocytes treated with the cross-linker dithiobis(succinimidyl propionate), lipoprotein lipase-containing complexes were isolated by anti-sortilin antibodies. To elucidate function in cells, sortilin-negative Chinese hamster ovary cells were transfected with full-length sortilin and shown to express about 8% of the receptors on the cell surface. These cells degraded 125I-labeled lipoprotein lipase much faster than the wild-type cells. The degradation was inhibited by unlabeled lipoprotein lipase, indicating a saturable pathway, and by RAP and heparin. Moreover, inhibition by the weak base chloroquine suggested that degradation occurs in an acidic vesicle compartment. The results demonstrate that sortilin is a multifunctional receptor that binds lipoprotein lipase and, when expressed on the cell surface, mediates its endocytosis and degradation. (+info)
Mutant vasopressin precursors that cause autosomal dominant neurohypophyseal diabetes insipidus retain dimerization and impair the secretion of wild-type proteins. (3/976)Autosomal dominant familial neurohypophyseal diabetes insipidus is caused by mutations in the arginine vasopressin (AVP) gene. We demonstrated recently that mutant AVP precursors accumulate within the endoplasmic reticulum of neuronal cells, leading to cellular toxicity. In this study, the possibility that mutant AVP precursors interact with wild-type (WT) proteins to alter their processing and function was explored. WT and mutant precursors were epitope-tagged to allow them to be distinguished in transfected cells. An in vivo cross-linking reaction revealed homo- and heterodimer formation between WT and mutant precursors. Mutant precursors were also shown to impair intracellular trafficking of WT precursors from the endoplasmic reticulum to the Golgi apparatus. In addition to the cytotoxicity caused by mutant AVP precursors, the interaction between the WT and mutant precursors suggests that a dominant-negative mechanism may also contribute to the pathogenesis of familial neurohypophyseal diabetes insipidus. (+info)
Evidence of interactions between the nucleocapsid protein NCp7 and the reverse transcriptase of HIV-1. (4/976)The human immunodeficiency virus (HIV-1) nucleocapsid protein NCp7 containing two CX2CX4HX4C-type zinc fingers was proposed to be involved in reverse transcriptase (RT)-catalyzed proviral DNA synthesis through promotion of tRNA3Lys annealing to the RNA primer binding site, improvement of DNA strand transfers, and enhancement of RT processivity. The NCp7 structural characteristics are crucial because mutations altering the finger domain conformation led to noninfectious viruses characterized by defects in provirus integration. These findings prompted us to study a putative RT/NCp7 protein-protein interaction. Binding assays using far Western analysis or RT immobilized on beads clearly showed the formation of a complex between NCp7 and RT. The affinity of NCp7 for p66/p51RT was 0.60 microM with a 1:1 stoechiometry. This interaction was confirmed by chemical cross-linking and co-immunoprecipitation of the two proteins in a viral environment. Competition experiments using different NCp7 mutants showed that alteration of the finger structure disrupted RT recognition, giving insights into the loss of infectivity of corresponding HIV-1 mutants. Together with structural data on RT, these results suggest that the role of NCp7 could be to enhance RT processivity through stabilization of a p51-induced active form of the p66 subunit and open the way for designing new antiviral agents. (+info)
Characterization of a haemolytic factor from Candida albicans. (5/976)The culture supernatant of Candida albicans promoted the disruption of human red blood cells (RBCs). The haemolytic activity was detected in a sugar-rich fraction (about 200 kDa) from Sephacryl S-100 chromatography. As the haemolytic activity was adsorbed by concanavalin A-Sepharose, the haemolytic factor may be a mannoprotein. The activity was inactivated by periodate oxidation, indicating that the sugar moiety of the mannoprotein played an important role in the haemolysis. The structure of the sugar moiety of the mannoprotein was identified as a cell-wall mannan by 1H-NMR analysis, and purified C. albicans mannan promoted the disruption of RBCs. The binding of mannan to RBCs was demonstrated by flow cytometric analysis and was inhibited by the addition of band 3 protein inhibitor, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. These results indicated that a mannoprotein released from C. albicans bound to the band 3 protein on RBCs, thereby promoting their disruption. (+info)
Analysis of the behaviour of selected CCKB/gastrin receptor antagonists in radioligand binding assays performed in mouse and rat cerebral cortex. (6/976)1. The previously described complex behaviour of the CCKB/gastrin receptor antagonist, L-365,260, in radioligand binding assays could be explained by a variable population of two binding sites. We have investigated whether other CCKB/gastrin receptor ligands (PD134,308, PD140,376, YM022 and JB93182) can distinguish between these sites. 2. In the mouse cortex assay, Hill slopes were not different from unity and the ligand pKI values did not differ when either [125I]-BH-CCK-8S or [3H]-PD140,376 was used as label as expected for a single site (G2). 3. In the rat cortex, where previous analysis of replicate (n=48) L-365,260 data indicated the presence of two CCKB/gastrin sites (G1 and G2), the competition data for PD134,308, PD140,376, YM022 and JB93182 could be explained by a homogeneous population of CCKB/gastrin sites because the Hill slope estimates were not significantly different from unity. However, the estimated affinity values for JB93182 and YM022 were significantly higher and that for PD134,308 was significantly lower than those obtained in the mouse cortex when the same radioligand was used. In view of our previous data obtained with L-365,260, the rat cortex data were also interpreted using a two-site model. In this analysis, SR27897 expressed approximately 9 fold, PD134,308 approximately 13 fold and PD140,376 approximately 11 fold selectivity for the G2 site. In contrast, JB93182 expressed approximately 23 fold and YM022 approximately 4 fold selectivity for the G1 site. If the two-site interpretation of the data is valid then, because of its reverse selectivity to L-365,260, JB93182 has been identified as a compound which if radiolabelled could provide a test of this receptor subdivision. (+info)
Thiolated recombinant human tumor necrosis factor-alpha protects against Plasmodium berghei K173-induced experimental cerebral malaria in mice. (7/976)The introduction of reactive thiol groups in recombinant human tumor necrosis factor (TNF) alpha (rhTNF-alpha) by the reagent succinimidyl-S-acetylthioacetate resulted in the formation of a chemically stabilized rhTNF-alpha trimer (rhTNFalpha-AT; as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis). rhTNFalpha-AT showed a substantially enhanced protective efficacy against the development of experimental murine cerebral malaria (ECM) after intravenous injection compared to the protective efficacy of nonmodified rhTNF-alpha. Administration of thiolated rhTNF-alpha with protected thiol groups (rhTNFalpha-ATA; no stabilized trimers in vitro) exhibited the same protective efficacy against ECM, while in vitro bioactivity was reduced. Parasitemia was significantly suppressed in rhTNF-treated mice that were protected against ECM but not in treated mice that developed ECM. Protection against ECM was not related to increased concentrations in plasma of soluble TNF receptor 1 and 2 directly after injection or at the moment of development of ECM in nontreated mice. The results indicate that thiolation of rhTNF-alpha leads to the formation of stable trimers with increased potential in vivo. (+info)
B lymphocytes as antigen-presenting cells for CD4+ T cell priming in vivo. (8/976)The contribution of B lymphocytes as APCs for CD4+ T cell priming remains controversial, based on findings that B cells cannot provide the requisite ligating and costimulatory signals for naive T cells to be activated. In the current study, we have examined Ag-specific T:B cell collaboration under circumstances in which B cells take up Ag through Ig receptors in vivo. This results in their activation and an ability to effectively stimulate naive CD4+ T cells both in vitro and in vivo. The aim of this work was to establish some of the key molecular interactions, as well as kinetics, between Ag-specific T and B cells that enable this priming to take place. Our approach was to amplify the starting pools of both Ag-specific T and B cell populations in vivo to track directly the events during initial T:B cell collaborations. We show that the induction of optimal levels of T cell priming to a protein Ag requires the involvement of Ag-specific B cells. The interaction that results between Ag-specific T and B cells prevents the down-modulation of B7 costimulatory molecules usually observed in the absence of appropriate T cells. Moreover, this prevention in down-modulation is independent of CD40:CD40 ligand contact. Finally, we present data suggesting that once Ag-specific T and B cells interact, there is a rapid (1-2-h) down-regulation of antigenic complexes on the surface of the B lymphocytes, possibly to prevent them from engaging other T cells in the vicinity and therefore focus the initial interaction. (+info)
Intramolecular crosslinking of hemoglobin A by sulfosuccinimidyl suberate: Application of crosslinked protein as a blood...
TY - JOUR. T1 - Intramolecular crosslinking of hemoglobin A by sulfosuccinimidyl suberate. T2 - Application of crosslinked protein as a blood substitute. AU - Manjula, B. N.. AU - Acharya, A. S.. PY - 1994/11. Y1 - 1994/11. N2 - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the appropriate length of the alkyl chain optimal for the crosslinking reaction.. AB - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the ...
Carboxyfluorescein succinimidyl ester - Wikipedia
Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Also, due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. CFSE is commonly confused with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), although they are not strictly the same molecule; CFDA-SE, due to its acetate groups, is highly cell permeable, while CFSE is much less so. As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration within ...
Sulfo-NHS Crosslinker 500 mg (N-hydroxysulfosuccinimide, CAS 106627-54-7) - N-hydroxysulfosuccinimide (Sulfo-NHS) - ProteoChem
Sulfo-NHS Crosslinker 500 mg $116.00 for bead conjugation, such as Luminex beads. Sulfo-NHS (N-hydroxysulfosuccinimide, CAS 106627-54-7) and EDC-HCl are used together in crosslinking reactions, and Sulfo-NHS improves conjugation efficiency. - Sulfo-NHS crosslinker (N-hydroxysulfosuccinimide) is used with EDC-HCl (EDAC-HCl) to convert carboxyl groups to Sulfo-NHS esters which react with primary amines. -
BS3 1 gram - Bis(sulfosuccinimidyl) suberate (BS3) - ProteoChem
BS3 crosslinker packaged as one gram per vial. Bulk quantities of BS3 are also available from ProteoChem. - BS3 crosslinking reagent, Bis(sulfosuccinimidyl) suberate, is a water soluble, membrane impermeable, homobifunctional protein crosslinking reagent with an 8-atom non-cleavable spacer arm. -
BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™ 5g BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™
BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™ 5g BOC-Glycine N-hydroxysuccinimide ester, 99%, ACROS Organics™ Amino Acids
215597-96-9 | BIS(SULFOSUCCINIMIDYL)SUCCINATE SODIUM SALT - Chemical Cloud Database
215597-96-9,CCD No.:CCD00211814,Formula:C12 H10 N2 O14 S2 . 2 Na,MolWeight:516.323,Synonyms：BIS(SULFOSUCCINIMIDYL)SUCCINATE SODIUM SALT; Molecular Structure,Chemical Cloud Database
Adoptive Transfers of CD4+CD25+ Tregs Raise Foxp3 Expression and Alleviate Mouse Enteritis
CD4|sup|+|/sup|CD25|sup|+|/sup|Foxp3|sup|+|/sup| Tregs control the immune response and maintain immune homeostasis. This study examined whether Tregs can affect mouse enteritis and the Foxp3 (Forkhead transcription factor) transcriptional pathway. Mouse CD4|sup|+|/sup|CD25|sup|+|/sup| Treg cells were labelled using CFSE (5,6-carboxyfluorescein diacetate succinimidyl ester) and transferred to enteritis model mice. The mice were randomly divided into an enteritis group, a Treg-infusion group, a Treg-inhibiting group, and a control group. Histopathology, ELISA, flow cytometry, western blot, immunohistochemistry, and immunofluorescence were performed. Our results demonstrated that CD4|sup|+|/sup|CD25|sup|+|/sup| Tregs were successfully transferred. The disease activity index (DAI) scores in the Tregs-infusion group were lower than those of the enteritis and Tregs-inhibiting groups. The number of goblet cells and inflammatory cells was reduced, and the levels of IL-1|i|β|/i|, TNF-|i|α|/i|, NO,
JCI - Resident lung antigen-presenting cells have the capacity to promote Th2 T cell differentiation in situ
Animals. All mice were used at 6-8 weeks of age. C57BL/6 mice were purchased from the National Cancer Institute (Frederick, Maryland, USA). A breeding pair of lymphotoxin-α (LTα) knockout mice (on a mixed 129 × C57BL/6 background) were originally obtained from David Chaplin (Washington University, St. Louis, Missouri, USA) and have since been backcrossed for more than eight generations onto a C57BL/6 background. For some experiments, LTα knockout mice were splenectomized 2 weeks prior to immunization. Splenectomies were performed by staff of the Veterinarian Care Services at Yale University.. Antigens. L. major promastigotes of the WR309 substrain were maintained at 23°C in Schneiders Drosophila medium (GIBCO BRL; Invitrogen Corporation, Grand Island, New York, USA) supplemented with 20% FCS and 50 μg/ml gentamicin. For some experiments, L. major parasites were labeled with CFSE (5- and 6-carboxyfluorescein diacetate succinimidyl ester; Molecular Probes Inc., Eugene, Oregon, USA) using a ...
Molecular Probes™ Alexa Fluor™ 750 NHS Ester (Succinimidyl Ester) 3 x 100μg Molecular Probes™ Alexa Fluor™ 750 NHS Ester ...
Shop a large selection of Protein Labelling Reagents products and learn more about Molecular Probes™ Alexa Fluor™ 750 NHS Ester (Succinimidyl Ester) 3 x 100μg
Acetic acid N-hydroxysuccinimide ester (CAS 14464-29-0) Market Research Report 2017
Acetic acid N-hydroxysuccinimide ester (CAS 14464-29-0) Market Research Report 2017 aims at providing comprehensive data on acetic acid n-hydroxysuccinimide
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* DYE-47 - Creative BioMart
Purified 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* from Creative Biomart. 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* can be used for research.
Synthesis of Aryl Thioethers Through the NCS-Promoted Cross-Coupling Reaction of Thiols with Grignard Reagents | NCHU...
A convenient one-pot approach for the synthesis of aryl sulfides through the coupling of thiols with Grignard reagents in the presence of N-chlorosuccinimide is described. The sulfenylchlorides were formed when thiols were treated with N-chlorosuccinimide, and the resulting sulfenylchlorides were then directly reacted with Grignard reagents to provide aryl sulfides in good to excellent yields under mild reaction conditions. Functional groups including ester, fluoro and chloro are tolerated by the reaction conditions employed. It is important to note that this method has a short reaction time (30 min in total), and represents an alternative approach for the synthesis of aryl sulfides over the existing protocols ...
CAS No.6066-82-6,N-Hydroxysuccinimide Suppliers,MSDS download
Find quality suppliers and manufacturers of 6066-82-6(N-Hydroxysuccinimide) for price inquiry. where to buy 6066-82-6(N-Hydroxysuccinimide).Also offer free database of 6066-82-6(N-Hydroxysuccinimide) including MSDS sheet(poisoning, toxicity, hazards and safety),chemical properties,Formula, density and structure, solution etc.
Z-L-Valine N-hydroxysuccinimide ester - Creative Peptides
Creative Peptides offers Z-L-Valine N-hydroxysuccinimide ester for your research. We also provide custom peptide synthesis, process development, GMP manufacturing.
The agonist binding site on the bovine bradykinin B2 receptor is adjacent to a sulfhydryl and is differentiated from the...
Chemical cross-linking was used to analyze the binding sites for the agonist bradykinin (BK) and the antagonists NPC17731 and HOE140 on the bovine B2 bradykinin receptor. [3H]BK and [3H]NPC17731 bound with high affinity to the same B2 receptor in bovine myometrial membranes as determined by the total number of specific binding sites and pharmacological specificity of the binding of these two radioligands. Cross-linking experiments were done using a series of bifunctional reagents reactive either primarily to amines (homobifunctional) or reactive to amines in one end and to sulfhydryls in the opposite end (heterobifunctional). All the heterobifunctional reagents plus the homobifunctional arylhalide 1,5-difluoro-2,4-dinitrobenzene were effective in cross-linking the [3H]BK N terminus specifically to a sulfhydryl in the receptor, and this cross-linking occurred at 5-100 μM reagent. In contrast, the homobifunctional N-hydroxysuccinimide ester reagents, at ≤1 mM, were only able to cross-link ...
Bis(sulfosuccinimidyl) suberatesodium salt - Annex III inventory - ECHA
ECHA compiled an inventory of substances likely to meet the criteria of Annex III to the REACH Regulation. The aim is to support registrants in identifying whether reduced minimum information requirements or a full Annex VII information set is required.. The inventory was produced using publicly available databases with experimental data and by using (Q)SAR model results. Indications for hazardous toxicological or ecotoxicological properties together with information on uses and other available relevant information have to be compared with the criteria in Annex III.. The fact that a substance is not in this list does not necessarily mean that the criteria for Annex III are not met. Likewise, if a substance is on this inventory, a registrant can still benefit from the reduced information requirements if it is justified.. Note that the inventory is not a tool for classification, it only shows indications for concern. For instance, the fact that a substance is indicated as Suspected mutagen does ...
Gentaur Molecular :Biotium \ NEP SUCCINIMIDYL ESTER \ 92204-1
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Gentaur Molecular :Biotium \ CF 770, succinimidyl ester \ 92150
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Thermo Scientific DSS (disuccinimidyl suberate), No-Weigh Format 50mg:Life | Fisher Scientific
Shop a large selection of Crosslinking Reagents products and learn more about Thermo Scientific DSS (disuccinimidyl suberate), No-Weigh Format 50mg:Life 50mg.
Fmoc-N-amido-dPEG®₈-NHS ester - Quanta BioDesign
Fmoc-N-amido-dPEG®8-NHS ester, product number 10995, is one of Quanta BioDesigns peptide synthesis products. This product is pre-activated with an N-hydroxysuccinimide (NHS) ester for conjugation to free amines. The dPEG®8 spacer allows the introduction of a short, hydrophilic spacer into a peptide chain. Read More PEGylation with Quanta BioDesign's dPEG® Products PEGylation refers to the covalent addition of polyethylene glycol (PEG) to a compound or surface. PEGylated compounds include proteins, peptides, dyes or other labels, and small molecule drugs. Peptide synthesis frequently employs PEGylation to enhance the water solubility of hydrophobic peptides. Moreover, peptide PEGylation prolongs the in vivo circulation of peptide conjugates by both increasing the peptide's hydrodynamic volume and protecting the peptide from proteolysis. Additionally, PEGylation reduces or eliminates the antigenicity of the conjugated peptide. Traditional PEGs, however, are dispersed polymers (Đ | 1
Materials. Bis(sulfosuccinimidyl)suberate (BS3), disuccinimidyl suberate (DSS), and NHS-SS-biotin were obtained from Pierce (Rockford, IL). Potassium hydroxide (99%) was from Aldrich (Milwaukee, WI). Type IV collagen was from Collaborative Biomedical Products (Bedford, MA). Normal goat serum and Vectastain mounting medium were from Vector Laboratories (Burlingame, CA). Except as noted, Sigma (St. Louis, MO) was the source of all other reagents and chemicals.. NGF was prepared as described previously (Mobley et al., 1986). NGF was labeled with 125I (Amersham, Arlington Heights, IL) using lactoperoxidase, as modified from Vale and Shooter (1985). Iodinated protein was separated from free 125I on a PD-10 column (Pharmacia, Uppsala, Sweden) preequilibrated with binding buffer [PBS, pH 7.4, containing 1 mg/ml glucose and 1 mg/ml bovine serum albumin (BSA)]. Final specific activity was 25-100 cpm/pg. Radioactivity was quantified using a Beckman 2000 gamma counter.. 1088 is a rabbit antibody against ...
Modelling deuterium labelling | Journal of The Royal Society Interface
Despite great advances in immunological research during the last decades, estimating the kinetics of lymphocyte populations has proved quite difficult. There are widely divergent estimates of the production rates, division rates and lifespans of mouse and human lymphocyte populations . As a consequence, it is poorly understood how memory is maintained, how the naive lymphocyte repertoire remains diverse and how homeostasis is brought about. Additionally, it remains poorly understood how viruses such as HIV, and diseases such as rheumatoid arthritis, disturb the normal population dynamics and deteriorate the functioning of the immune system.. Several in vivo labelling techniques have been developed that have enabled the generation of quantitative data on lymphocyte dynamics. Examples of labels are the fluorescent dye carboxy-fluorescein diacetate succinimidyl ester (CFSE), the base analogue 5-bromo-2′-deoxyuridine (BrdU) and the stable isotype deuterium. Major advantages of deuterium ...
BDP TR X NHS ester
BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX (Texas Red). The NHS ester function can be used for the conjugation with proteins and peptides.
chloramine-b: Topics by WorldWideScience.org
Rates of Decomposition of N-Chloramine Disinfectant Compounds in Aqueous Solutions. International Nuclear Information System (INIS) EI-Bellihi, E.E.. 2009-01-01. The effect of temperature, ph, and salt effects on the decomposition kinetics of hydrolysis of N-chloramine disinfectant compounds [chloramine-B, chloramine-T, N-chlorosuccinimide (NCS), and 1,3-dichloro-5,5-dimethyl hydantoin (DCDMH or Halane)] in aqueous solutions was studied. The results should that the hydrolytic stability of CB and CT is greater than that of NCS and halane. Using CT, which is practical in use for its long contact times, reduced its initial concentration in aqueous solution from 100 ppm to about 20 ppm after a period of 6 months. The study also showed that the rate of hydrolysis of NCS is almost independent on the H + ions concentration. On the other hand, the rates of hydrolysis of CB and CT depend strongly on the hydrogen ion (H + ) concentration where the kinetic of the reaction changes from zero-order to a first ...
Authors: ASGHAR DAVOOD, ESKANDAR ALIPOUR, ABBAS SHAFIEE Abstract: 4(5)-Chloro-imidazole-5(4)-carboxaldehyde derivatives are important precursors for the preparation of biologically active compounds. We developed a simple, novel, and efficient method for the synthesis of these compounds. The chemistry described is amenable to large-scale use and is flexible enough to allow the preparation of analogs. Keywords: Imidazole, N-chlorosuccinimide, chloroimidazole, chloroimidazole-carboxaldehyde Full Text: PDF ...
Elucidation of the quaternary structure of the insulin receptor | Biochemical Journal
Photoreactive insulin analogues specifically label predominantly one polypeptide in the insulin receptor of rat liver plasma membranes. We have used the bifunctional reagent disuccinimidyl suberate to cross-link this polypeptide to its neighbouring, but not necessarily labelled, subunits. The results of these studies show that (1) there are at least three types of subunit in the receptor, with apparent Mr (Mapp.) values of 65 000, 95 000 and 120 000; (2) the receptor appears to consist of two Mapp. 120 000, one Mapp. 95 000 and one Mapp. 65 000 subunits; (3) the Mapp. 65 000 subunit, which has not been previously reported, may be only loosely attached to the receptor, and does not interact directly with the insulin-binding subunit (M app. 120 000). ...
Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98% 250mg Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98%
Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98% 250mg Alfa Aesar™ N-Boc-L-phenylalanine N-succinimidyl ester, 98% Amino Acids
MB Succinimidyl Ester | Biotium
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. See the product information sheets for specific reactive dyes more information.. Coelenterazines and D-luciferin. Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also ...
BOLTON-HUNTER REAGENT, [125I] (0165015) - MP Biomedicals
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
BioProduct: Molecular Biology: Molecular Probes: Cy5 NHS ester: Cy5 NHS ester
Reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Can replace Alexa Fluor 647, DyLight 649 ...
产品名称：Acid-PEG8-NHS ester货号：BAPN-7品牌：BioconeCAS: 分子式: C24H41NO14分子量: 567.59纯度：95% 以上 包装规格：100 mg、500mg、1g、5g、10g等，其...
IR 680LT NHS Ester | Fluoroprobes
IR Fluor 680 (IRDye® 680 equivalent) is a bright, near-IR fluorescent, amine-reactive dyes used for labeling primary amines of proteins.
Bright lights for live cells
To assess the efficacy of their method, the team mixed the aldehydes with brain cancer cells in vitro for 10 minutes then compared them with typical amino reactive dye precursors known as succinimidyl esters (NHS) (Fig. 1). They discovered that the aldehyde precursors produced brighter fluorescence than the NHS dyes. Confocal microscopy showed that the aldehydes reacted with amino groups of lysine amino acid residues on the cell surface and those of other cell membrane components, whereas the NHS dyes penetrated the cells. They confirmed this by treating the labeled cells with detergent: the aldehyde-derived labels washed off the cell surface, whereas their NHS counterparts remained in the cells. The aldehyde-derived labels also remained effective at exceptionally low concentrations, unlike the NHS-derived labels. In contrast to pre-existing cell labeling protocols, this reaction tightly anchors the labels to the surface of living cells within 10 minutes at 10 nM [dye] concentrations and with a ...
Fluorescent Dyes , Reactive Fluorescent Dyes , 5(6)-TAMRA-X, SE; TAMRA-X contains a seven-atom aminohexanoyl spacer (known as X ) between TAMRA fluorophore and the succinimidyl ester. The X spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. We recommend this TAMRA-X derivative as the preferred dye for preparing TAMRA-labeled proteins when the fluorescence quenching of the labeling dye by protein is a serious problem.; C35H36N4O8
Block of T -Type Ca2+ Channels Is an Important Action of Succinimide Antiabsence Drugs - Huguenard - 2002 - Epilepsy Currents -...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
Propargyl-PEG6-NHS ester | calpaininhibitor.com
Product Name: Propargyl-PEG6-NHS esterFormula: C20H31NO10MW: 445.5Web Site：MedchemexpressPurity: 0.98Availability: In StockCAS NO: 1403254-99-8 Product: EPZ-6
DNP-dPEG®₄-NHS ester - Quanta BioDesign
Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry 180 reactions Invitrogen™ CellTrace™ CFSE Cell...
Shop a large selection of Cell Growth and Differentiation Reagents and Kits products and learn more about Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit,
VivoTag-S 750 Fluorochrome (5mg) | PerkinElmer
VivoTag-S 750 amine-reactive near-infrared fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules. 5 mg quantity.
Mesenchymal stem cells activate Notch signaling to induce regulatory dendritic cells in LPS-induced acute lung injury | Journal...
Mesenchymal stem cells (MSCs) have been shown to alleviate acute lung injury (ALI) and induce the production of regulatory dendritic cells (DCregs), but the potential link between these two cell types remains unclear. The goal of this study was to investigate the effect and mechanism of MSC-induced regulatory dendritic cells in ALI mice. In vivo experiments, C57BL/6 wild-type male mice were sacrificed at different times after intratracheal injection of LPS to observe changes in lung DC maturation and pathological damage. MSCs, DCregs or/and carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled DCs were administered to the mice by tail vein, and flow cytometry was performed to measure the phenotype of lung DCs and T cells. Lung injury was estimated by the lung wet weight/body weight ratio and histopathological analysis. In vitro, Western blotting or flow cytometry was used to detect the expression of Notch ligand or receptor in MSCs or DCs after coculture or LPS stimulation. Finally, in vivo and
Effect of Nickel Chloride on Cell Proliferation
Objective: Metal alloys used in dentistry and in other biomedical fields may release nickel ions in the oral environment. The release of nickel might influence the normal biological and physiological processes, including tissue wound healing, cell growth and proliferation. The aim of this study was to evaluate in vitro the effects of nickel ions on cell cycle, viability and proliferation. Materials and Methods: Human osteosarcoma cells (U2OS) and human keratinocytes (HaCat) were exposed to different nickel chloride (NiCl2) concentrations (0 - 5mM) for various periods exposure. The viability of cultured cells was estimated by flow cytometry using Annexin V-FITC and Propidium Iodide (PI). Cell proliferation was evaluated by using carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and flow cytometry. Finally, the effects of NiCl2 on cell cycle were assessed and quantified by flow cytometry. Statistical analysis was performed by means of ANOVA followed by Tukeys test. Results: NiCl2 induced ...
IJMS | Free Full-Text | Racemization of the Succinimide Intermediate Formed in Proteins and Peptides: A Computational Study of...
In proteins and peptides, d-aspartic acid (d-Asp) and d-β-Asp residues can be spontaneously formed via racemization of the succinimide intermediate formed from l-Asp and l-asparagine (l-Asn) residues. These biologically uncommon amino acid residues are known to have relevance to aging and pathologies. Although nonenzymatic, the succinimide racemization will not occur without a catalyst at room or biological temperature. In the present study, we computationally investigated the mechanism of succinimide racemization catalyzed by dihydrogen phosphate ion, H2PO4−, by B3LYP/6-31+G(d,p) density functional theory calculations, using a model compound in which an aminosuccinyl (Asu) residue is capped with acetyl (Ace) and NCH3 (Nme) groups on the N- and C-termini, respectively (Ace-Asu-Nme). It was shown that an H2PO4− ion can catalyze the enolization of the Hα-Cα-C=O portion of the Asu residue by acting as a proton-transfer mediator. The resulting complex between the enol form and H2PO4− corresponds to
The murine haemopexin receptor. Evidence that the haemopexin-binding site resides on a 20 kDa subunit and that receptor...
Haemopexin receptors from mouse hepatoma (Hepa) cells were affinity-labelled by cross-linking to haem-125I-haemopexin complexes using two homo-[disuccinimidyl suberate (DSS) and 3,3′-dithiobis(succinimidyl propionate) (DTSSP)] and one hetero-[sulphosuccinimidyl 4-(p-maleimidophenyl)butyrate (sulpho-SMPB)] bifunctional cross-linking agents. Analysis of the cross-linked products by SDS/PAGE in the absence of reducing agents revealed that 125I-haemopexin was cross-linked specifically to a protein of apparent molecular mass 85-90 kDa. Upon reduction, haemopexin remained cross-linked to a protein of 20 kDa, suggesting that the murine haemopexin receptor has a subunit structure. Two subunits were identified: alpha (p65) and beta (p20). Furthermore, because haemopexin was cross-linked by all three agents to p20, the shortest cross-linker arm being 1.1 nm (11 A), we propose that the haem-haemopexin-binding site resides on this subunit. In addition, a cysteine residue of p20 is located near the ...
Targeting the allergen to oral dendritic cells with mucoadhesive chitosan particles enhances tolerance induction - Saint-Lu -...
Background: Sublingual immunotherapy (SLIT) efficacy could be improved by formulations facilitating allergen contact with the oral mucosa and uptake by antigen-presenting cells (APCs).. Methods: Two types of chitosan microparticles, differing in size and surface charge, were tested in vitro for their capacity to improve antigen uptake and presentation by murine bone marrow-derived dendritic cells (BMDCs) or purified oral APCs. T-cell priming in cervical lymph nodes (LNs) was assessed by intravenous transfer of carboxyfluorescein diacetate succinimidyl ester-labelled ovalbumin (OVA)-specific CD4+ T cells and flow cytometry analysis. Ovalbumin-sensitized BALB/c mice were treated sublingually with soluble or chitosan-formulated OVA twice a week for 2 months. Airway hyperresponsiveness (AHR), lung inflammation and T-cell responses in cervical and mediastinal LNs were assessed by whole-body plethysmography, lung histology and Cytometric Bead Array technology, respectively.. Results: Only a ...
RİVOTRİL NEDİR VE RİVOTRİLİN YAN ETKİLERİ NELERDİR | Eyeportal
Rivotril belongs to a class of drugs known as benzodiazepines .Rivotril is used alone or along with other medications to treat convulsive disorders such as epilepsy. Clonazepam is metabolized by the liver to inactive metabolites, which are excreted mainly in the urine. It is also prescribed for panic disorder--unexpected attacks of overwhelming panic accompanied by fear of recurrence.. I n this multicenter, parallel-group, placebo-controlled, fixed-dose study, the efficacy, safety, dosing characteristics, and discontinuation of clonazepam were analyzed in patients with panic disorder.. Rivotril is useful alone or as an adjunct in the treatment of the Lennox-Gastaut syndrome (petit mal variant), akinetic and myoclonic seizures. In patients with absence seizures (petit mal) who have failed to respond to succinimides, Rivotril may be useful. ...
Induction of Triple-negative Breast Cancer Cells to Immunogenic Cell Death and Increase Cross-Presentation by Streptomyces...
Abstract:. Background: Biomass extract of Streptomyces sanyensis (BE-SS), which is a marine microorganism, has antitumor activity and has white-to-gray aerial mycelium and long chains of long spores in aerial mycelium. Objectives: The anti-cancer effect of BE-SS on triple-negative breast cancer (TNBC) was confirmed and cell death by BE-SS was confirmed to have immunogenicity and whether it could be used for immuno-cancer therapy. Materials and Methods: Human and mouse TNBC cells (MDA-MB-231, HS578T, 4T1-hemagglutinin (HA) and TUBO-P2J-HA cells) were treated with BE-SS at different concentrations for 72 hr and their cytotoxicity was detected using the sulforhodamine B-based (SRB) method. Flow cytometry was used to determine the type of cell death by Annexin V/7-AAD staining and to measure surface exposure to damage-related molecular pattern (DAMP) molecules including calreticulin (CRT), heat shock protein (HSP) 70/90. Carboxyfluorescein succinimidyl ester (CFSE) dilution assay was used to ...
A serum-induced 29 Kd protein of mouse embryo fibroblasts is tightly bound to the chromatin - Publications of the IAS Fellows
Treatment of quiescent cultures of mouse embryo fibroblasts with 20% fetal calf serum (FCS) or cycloheximide (CH) resulted in the induction of a nuclear protein of molecular weight 29 000 daltons. The 29 Kd protein induced by these two agents was found to be tightly bound to the chromatin since it was not released from the chromatin by a combination of 2.5 M NaCl and 3.2 M urea. The chromatin associated CH-induced 29 Kd protein and the 29 Kd protein obtained from serum-induced cells displayed similar N-chlorosuccinimide cleavage patterns. Pulse-chase experiments indicate a half-life of an hour for the 29 Kd protein. The kinetics of induction of the 29 Kd protein in the early hours of mitogen addition, short half-life, nuclear localisation and strong association with chromatin suggest that this protein may have important roles in cell proliferation, possibly as a mediator of mitogen action. ...
Can methsuximide be given to children? | Anticonvulsant - Sharecare
Yes, methsuximide may be given to children. Methsuximide (Celontin) is an anticonvulsant medication that controls absence seizures by decreasing the a
Brevet US4906252 - Polyolefinic succinimide polyamine alkyl acetoacetate adducts as dispersants ... - Google Brevets
This invention is to a fuel oil composition containing polyolefinic succinimide polyamine alkyl acetoacetate adducts of the general following: ##STR1## wherein the variables are defined herein. The adduct additives are especially useful in concentrates and fuel oil compositions as dispersants. The formulations have excellent cold flow properties compared to similar formulations not containing the inventive adduct.
Probing GPCR conformation in cell by chemical crosslinking and mass spectrometry | NIH Research Festival
The activation of G protein-coupled receptors (GPCR) mediates a variety of important biological signals. GPR110 belongs to the adhesion GPCR class with distinctively long N-terminus. Although GPR110 is an orphan receptor with unknown ligand, it has been identified as an oncogene implicated in lung and prostate cancers. In an effort to understand the molecular basis of GPR110 activation, we probed GPR110 conformation in living cells using chemical crosslinking and mass spectrometry. HEK cells overexpressing HA-GPR110 were incubated with disuccinimidyl suberate (DSS, a lysine-specific crosslinker). The DSS-modified HA-GPR110 was pulled-down and subjected to SDS-PAGE, tryptic digestion, and nanoLC-ESI-MS/MS. The MS-based approach identified 17 crosslinked lysine pairs in the N-terminal domain of GPR110. Among them, K29-K38, K187-K240, K240-K254, K398-442, K398-K438, K398-K427, K427-K442, K427-K438, and K151-K442, resulted from through-space crosslinking between two peptide segments, while K31-K32, ...
Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, N2-Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labeled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide. O ur repertoire of NHS ester derivatives has been expanded to include the NHS-Carboxy-dT-CE Phosphoramidite. By making a dT analog of the ...
First Atropo-Enantioselective Ring Opening of Achiral Biaryls Containing Lactone Bridges with Chiral Hydride-Transfer...
4] a ) J. T. Kauer, S. Erickson-Vitanen, H. R. Wolfe, W. F. De Grddo, J B d . Chrm. 1986.261. 10695, b) W. T. Miller, E. T. Kaiser. Proc. Nurl. Arud. Sci. U S A 1988, K S , 5429. c) T. Tao. C. J. Schemer, M. Lamkin. 5io~liemistr.v 1986.2.7. 7633.  a ) R. Breslow, Acr. Chrm. Res. 1980, 13, 170, b) C . Helene, Phofochem. Pholohiol. 1972, 16. 519.  G. W. Anderson, J. E. Zimmerman. F. M. Gallahan, J. Am. Cheni. Sor. 1964. K6. 1839.  A section of a silica substrate bearing nitroveratryloxycarbonyl(NV0C)protected amino groups was photodeprotected  and then derivatized uith the NHS ester of 3-benzoylbenzoic acid. A second section of the substrate was then deprotected and derivatized with the NHS ester of biotin. Any unreacted sites in the first section (due to incomplete coupling of benzophenone) will also react with the biotin NHS ester. The entire surface was then treated with fluorescein-streptavidin (Molecular Probes lnc.. Eugene. O R (USA)). The coupling efficiency of benzophenone was ...
PEG PFP ester - Amine reactive, PEGylation, PEGylation Reagent, PEG reagent | BroadPharm
PEG PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. It can be used either in homobifunctional or heterobifunctional labelling such as surface modification on nanoparticles and cells ...
Université Francois Rabelais - Tours - In Vitro Characterization and Stability Profiles of Antibody-Fluorophore Conjugates...
Fluorescent labelling of monoclonal antibodies (mAbs) is classically performed by chemical bioconjugation methods. The most frequent labelling technique to generate antibody-fluorophore conjugates (AFCs) involves the bioconjugation onto the mAb lysines of a dye bearing an N-hydroxysuccinimide ester or an isothiocyanate group. However, discrepancies between labelling experiments or kits can be observed, related to reproducibility issues, alteration of antigen binding, or mAb properties. The lack of information on labelling kits and the incomplete characterization of the obtained labelled mAbs largely contribute to these issues. In this work, we generated eight AFCs through either lysine or interchain cysteine cross-linking bioconjugation of green-emitting fluorophores (fluorescein or BODIPY) onto either trastuzumab or rituximab. This strategy allowed us to study the influence of fluorophore solubility, bioconjugation technology, and antibody nature on two known labelling procedures. The structures of
N-HYDROXYSUCCINIMIDE || Skin Deep® Cosmetics Database | EWG
Beyond providing Skin Deep® as an educational tool for consumers, EWG offers its EWG VERIFIED™ mark as a quick and easily identifiable way of conveying personal care products that meet EWGs strict health criteria. Before a company can use EWG VERIFIEDTM on such products, the company must show that it fully discloses the products ingredients on their labels or packaging, they do not contain EWG ingredients of concern, and are made with good manufacturing practices, among other criteria. Note that EWG receives licensing fees from all EWG VERIFIED member companies that help to support the important work we do. Learn more , Legal Disclaimer ...
AnaTag AMCA - X Microscale Protein Labeling Kit *Ultra Convenient*
AnaTag Protein Labeling Kits , Classic Fluorescent Dye Labeling Kits , AnaTag AMCA - X Microscale Protein Labeling Kit *Ultra Convenient*; AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group. This spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. AMCA fluorescence can be detected at the emission wavelength of 442 10 nm when excited at 353 10 nm. The AMCA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the AMCA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction
AnaSpec Introduces AnaTag™ HiLyte Fluor™ 594 and 680 Protein Labeling Kits
AnaTag™ HiLyte Fluor™ Protein Labeling kits provide a way to label proteins using the succinimidyl ester reactive form of the HiLyte Fluor™ dyes.
Surfaces with primary or secondary amino groups covalently bound are dedicated to promote the covalent immobilisationof compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctionallinkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1-carboxylate (SMCC).. This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules tothe surfaces:. ...
Surfaces with primary or secondary amino groups covalently bound are dedicated to promote the covalent immobilisationof compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctionallinkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1-carboxylate (SMCC).. This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules tothe surfaces:. ...
CyLyte Fluor 7, NHS ester | Fluorescent Dyes | Fluorescence Technology | Products | MoBiTec Molecular Biotechnology
Your German Partner for Transfection, Gene Expression, Fluorescent Probes, IVD Kits, Antibodies, Peptides, Lipidomics, Glycobiology, Lab Supplies, and more.
SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)
Oral tolerance is a phenomenon that may occur in animals exposed to protein antigens for the first time by the oral route. They become unable to produce immune responses at the levels normally observed when they are immunized parenterally with antigen in the presence of adjuvants. Lipids have been used as adjuvants for both parenteral and oral immunization. In the present study we coupled ovalbumin with palmitate residues by incubating the protein with the N-hydroxysuccinimide palmitate ester and tested the preparation for its ability to induce oral tolerance. This was performed by giving 20 mg of antigen to mice by the oral route 7 days prior to parenteral immunization in the presence of Al(OH)3. Mice were bled one week after receiving a booster that was ...
Preparation of morphine-N-(ε-trifluoroacetylcaproyloxy | Open-i
Preparation of morphine-N-(ε-trifluoroacetylcaproyloxy) succinimide ester (TFCS)-keyhole limpet hemocyanin (KLH), showing conjugation of 6-glutarylmorphine (M-
PEG Linkers, PEGylation, PEGylation Reagents, PEGylation Tools| BroadPharm
Find more than 1700 high purity PEG linkers with a broad selection of funcitonal groups (amine, azide, maleimide, NHS ester, bromide, etc) to inspire your PEGylation. Order now!
BDY 630-X, SE Supplier | CAS 380367-48-6 | BDY630-X,SE | Tocris Bioscience
View and buy high quality BDY 630-X, SE from Tocris Bioscience. Red BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester.
PromoKine - PromoFluor Dyes
Reactive PromoFluor dyes (available as e.g. NHS esters or maleimides) for labeling of biomolecules such as proteins, antibodies and nucleic acids.
carboxyfluorescein loaded bolaamphiphilic nanovesicle
Page contains details about carboxyfluorescein loaded bolaamphiphilic nanovesicle . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Highly hydrophilic, amine-reactive label containing one NHS-ester group. A brighter and more photostable replacement for Alexa 633, with a much higher extinction coefficient (250,000) and brightness after labeling to proteins. ...
PERFORM Centre Booking: SpectroLinker (XL-1000)
The Spectrolinker XL-1000 UV crosslinker was made for applications including eliminating PCR contamination, cross-linking DNA an...
Image Bank | Lightsources
When a low concentrations of crosslinker is added to resist (a film used to lay down the patterns of ever-shrinking lines and features on a chip, shown here at left), it is able to pattern smaller ...
"Polyisobutylene Succinimides in Engine Oil". Lubrizol. Archived from the original on 2017-02-15. Retrieved 2017-02-14. J. Reid ... J. Barker (2013). Understanding Polyisobutylene Succinimides (PIBSI) and Internal Diesel Injector Deposits (Technical report). ...
ISBN 0-88048-681-3. Ishizumi K, Kojima A, Antoku F, Saji I, Yoshigi M (December 1995). "Succinimide derivatives. II. Synthesis ...
The following are succinimides: Ethosuximide (1955). Phensuximide. Mesuximide. Acetazolamide (1953). Sultiame. Methazolamide. ...
Researcher D. G. Walker of the University of Birmingham determined the presence of two specific enzymes in adult guinea pig liver, both of which catalyze the phosphorylation of glucose to glucose 6 phosphate.[dubious - discuss] The two enzymes have been identified as a specific glucokinase (ATP-D-glucose 6-phosphotransferase) and non-specific hexokinase (ATP-D-hexose 6-phosphotransferase). Hepatic cell is freely permeable to glucose, and the initial rate of phosphorylation of glucose is the rate-limiting step in glucose metabolism by the liver (ATP-D-glucose 6-phosphotransferase) and non-specific hexokinase (ATP-D-hexose 6-phosphotransferase). The role of glucose 6-phosphate in glycogen synthase: High blood glucose concentration causes an increase in intracellular levels of glucose 6 phosphate in liver, skeletal muscle and fat (adipose) tissue. (ATP-D-glucose 6-phosphotransferase) and non-specific hexokinase (ATP-D-hexose 6-phosphotransferase). In liver, synthesis of glycogen is directly ...
They are reacted with ethyleneamines to give the corresponding succinimides useful as dispersants in lubricants and fuels. and ... 16-19, 2010 "Polyisobutylene Succinimides in Engine Oil". Lubrizol. Archived from the original on 2017-02-15. Retrieved 2017-02 ... CS1 maint: discouraged parameter (link) Reid, Jacqueline; Barker, Jim (2013). "Understanding Polyisobutylene Succinimides ( ...
N-Methylmaleimide Succinimide Hermanson, Greg (2013). Bioconjugate Techniques. Elsevier. pp. 299-339. ISBN 978-0-12-382239-0. ...
The next year, they showed that anticonvulsant succinimides did this and that the pro-convulsant ones did not. The first part ... Ethosuximide is in the succinimide family of medications. How exactly it works is unclear. Ethosuximide was approved for ... "Block of cloned human T-type calcium channels by succinimide antiepileptic drugs". Molecular Pharmacology. 60 (5): 1121-32. doi ...
... is an anticonvulsant in the succinimide class. Diseases Database (DDB): 31648 Rankin G, Cressey-Veneziano K, Wang ...
... (or methsuximide, methosuximide) is a succinimide anticonvulsant medication. It is sold as a racemate by Pfizer ...
NCS is related to succinimide, but with NCl in place of NH. The N-Cl bond is highly reactive, and NCS functions as a source of ...
Examples are succinimide, derived from succinic acid, and phthalimide, derived from phthalic acid. For imides derived from ...
Stephenson, RC; Clarke, S (1989). "Succinimide Formation from Aspartyl and Asparaginyl Peptides as a Model for the Spontaneous ... Clarke, S (1987). "Propensity for spontaneous succinimide formation from aspartyl and asparaginyl residues in cellular proteins ... forming an asymmetric succinimide intermediate (in red). The asymmetry of the intermediate results in two products of its ... but at a much slower rate since formation of the six-member-ring glutarimide intermediate is less favoured than the succinimide ...
The ester rapidly and spontaneously turns into the succinimide (red), and randomly turns back into normal aspartic acid (black ... Stephenson RC, Clarke S (1989). "Succinimide Formation from Aspartyl and Asparaginyl Peptides as a Model for the Spontaneous ... Clarke S (1987). "Propensity for spontaneous succinimide formation from aspartyl and asparaginyl residues in cellular proteins ... forming a succinimide intermediate (in red). Hydrolysis of the intermediate results in two products, either aspartic acid (in ...
Succinimide. (Pyrrolidine-2,5-dione) Azide Azide RN3. azido-. alkyl azide Phenyl azide. (Azidobenzene) ...
Typical detergents are long-chain amines and amides such as polyisobuteneamine and polyisobuteneamide/succinimide. Reagent ...
Mercury Succinimide. *Mercury Imido-succinate. *Mercury Sulphate. *Basic Mercury Subsulphate; Turpeth Mineral ...
Nakato, Takeshi; Kusuno, Atsushi; Kakuchi, Toyoji (2000). "Synthesis of poly(succinimide) by bulk polycondensation of L- ... which yields partial opening of the succinimide rings. In this process sodium-DL-(α,β)-poly(aspartate) with 30% α-linkages and ...
Tannins, β-sitosterol, acalyphamide, aurantiamide, succinimide, and flindersin (a pyranoquinolinone alkaloid) have also been ...
Protein primary structure
deamidation (succinimide formation). In this modification, an asparagine or aspartate side chain attacks the following peptide ... bond, forming a symmetrical succinimide intermediate. Hydrolysis of the intermediate produces either aspartate or the β-amino ...
Succinate can be used to derive 1,4-butanediol, maleic anhydride, succinimide, 2-pyrrolidinone and tetrahydrofuran. In 2004, ...
These polymers have acryloyl, methacryloyl, maleimide, boronate and N‐hydroxy (sulfo) succinimide ester groups in their ...
The L-succinimide can then undergo nonenzymatic hydrolysis, which generates some repaired L-aspartyl residues as well as some L ... Subsequent nonenzymatic reactions result in a rapid transformation to L-succinimide, which is a precursor to aspartate and ...
Surface plasmon resonance microscopy
The attachment of HP was done through its amino groups to N-hydroxy succinimide functionalities on the gold surface. First SPRi ...
... a succinimide radical. This is spontaneously hydrolyzed either back to L-aspartyl or, in a more favorable reaction, to abnormal ...
Fluorescence in the life sciences
Fluorophores can be attached to proteins via specific functional groups, such as: amino groups (e.g. via succinimide or ...
Rxn of that product with succinic anhydride converts the pendant amine to a succinimide, affording the anxiolytic agent ...
... as in acetamide vs succinimide. Imines are related to ketones and aldehydes by replacement of the oxygen with an NR group. When ...
... for example succinimide. Tricarboxylic acid Boy Cornils, Peter Lappe "Dicarboxylic Acids, Aliphatic" in Ullmann's Encyclopedia ...
Once all N-bromosuccinimide (which is denser than the solvent) has been converted to succinimide (which floats on top) the ...
Among the various oxidants used are mercuric oxide, nitric acid, isoamyl nitrite, N-bromo succinimide, potassium permanganate, ...
Succinimide - Wikipedia
Succinimides refers to compounds that contain the succinimide group. These compounds have some notable uses. Several ... Succinimide is an organic compound with the formula (CH2)2(CO)2NH. This white solid is used in a variety of organic syntheses, ... Succinimides are also used to form covalent bonds between proteins or peptides and plastics, which is useful in a variety of ... ISBN 978-0-85404-182-4. Merck Index, 12th Edition, 9040 H. T. Clarke and Letha Davies Behr "Succinimide" Organic Syntheses 1936 ...
Succinimide - Registration Dossier - ECHA
Succinimide EC Number:. 204-635-6. EC Name:. Succinimide. CAS Number:. 123-56-8 Molecular formula:. C4H5NO2 IUPAC Name:. ... SuccinimideSuccinimide. CAS Number:. 123-56-8. Molecular formula:. C4H5NO2. IUPAC Name:. ... SuccinimideSuccinimide. CAS Number:. 123-56-8. Molecular formula:. C4H5NO2. IUPAC Name:. ... SuccinimideSuccinimide. CAS Number:. 123-56-8. Molecular formula:. C4H5NO2. IUPAC Name:. ...
Succinimides | Harvard Catalyst Profiles | Harvard Catalyst
"Succinimides" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Succinimides" by people in Harvard Catalyst Profiles by year, ... Detection and quantitation of succinimide in intact protein via hydrazine trapping and chemical derivatization. J Pharm Sci. ... Below are the most recent publications written about "Succinimides" by people in Profiles. ...
N-(Benzyloxycarbonyloxy)succinimide, 98%, ACROS Organics | Fisher Scientific
... succinimide, 98%, ACROS Organics 25g; Glass bottle Chemicals:Organic Compounds:Organoheterocyclic compounds:Pyrrolidines: ... n-benzyloxycarbonyloxy succinimide,cbz-osu,cbz-onsu,benzyl 2,5-dioxopyrrolidin-1-yl carbonate,benzyl succinimido carbonate,z- ... n-benzyloxycarbonyloxy succinimide,cbz-osu,cbz-onsu,benzyl 2,5-dioxopyrrolidin-1-yl carbonate,benzyl succinimido carbonate,z- ...
Cas 123-56-8,Succinimide | lookchem
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RCSB PDB - 1AT5: HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE Methods Report Page
Molecular Expressions: Science, Optics & You - Olympus MIC-D: Chemical Crystal Movie Gallery - Bromo-Succinimide Movie #1
Two-water-assisted racemization of the succinimide intermediate formed in proteins. A computational model study
In the widely accepted mechanism of the Asp racemization, a succinimide (SI) intermediate is the species which actually undergo ... "Two-water-assisted racemization of the succinimide intermediate formed in proteins. A computational model study" written by ... Phosphate-Catalyzed Succinimide Formation from an NGR-Containing Cyclic Peptide: A Novel Mechanism for Deammoniation of the ... Racemization of the Succinimide Intermediate Formed in Proteins and Peptides: A Computational Study of the Mechanism Catalyzed ...
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Epilepsy and Seizures Medication: Anticonvulsants, Other, Anticonvulsants, Barbiturates, Anticonvulsants, Benzodiazepine,...
Straightforward Access to Densely Substituted Chiral Succinimides through Enantioselective Organocatalyzed Michael Addition of...
A simple organocatalytic system provides efficient access to a series of densely substituted chiral succinimides bearing a ... A simple organocatalytic system provides efficient access to a series of densely substituted chiral succinimides bearing a ... Straightforward Access to Densely Substituted Chiral Succinimides through Enantioselective Organocatalyzed Michael Addition of ... Straightforward Access to Densely Substituted Chiral Succinimides through Enantioselective Organocatalyzed Michael Addition of ...
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Although nonenzymatic, the succinimide racemization will not occur without a catalyst at room or biological temperature. In the ... and d-β-Asp residues can be spontaneously formed via racemization of the succinimide intermediate formed from l-Asp and l- ... present study, we computationally investigated the mechanism of succinimide racemization catalyzed by dihydrogen phosphate ion ... Although nonenzymatic, the succinimide racemization will not occur without a catalyst at room or biological temperature. In the ...
IJMS | Free Full-Text | Acetic Acid Can Catalyze Succinimide Formation from Aspartic Acid Residues by a Concerted Bond...
Carboxylic acids other than AA are also expected to catalyze the succinimide formation by a similar mechanism. ... catalyzes the succinimide formation from Asp residues by acting as a proton-transfer mediator. The proposed mechanism comprises ... Succinimide formation from aspartic acid (Asp) residues is a concern in the formulation of protein drugs. Based on density ... Carboxylic acids other than AA are also expected to catalyze the succinimide formation by a similar mechanism. View Full-Text ...
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Geiger, T. and Clarke, S. (1987) Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides....
Succinimide-linked reactions that contribute to protein degradation. Journal of Biological Chemistry, 262, 785-794. ... In the widely accepted mechanism of the Asp racemization, a succinimide (SI) intermediate is the species which actually undergo ... TITLE: Two-water-assisted racemization of the succinimide intermediate formed in proteins. A computational model study ... KEYWORDS: Aspartic Acid Residue; Racemization; Nonenzymatic Reaction; Succinimide; Water Catalysis; Enolization; Computational ...
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Used in the treatment of epilepsy. Ethosuximide suppresses the paroxysmal three cycle per second spike and wave activity associated with lapses of consciousness which is common in absence (petit mal) seizures. The frequency of epileptiform attacks is reduced, apparently by depression of the motor cortex and elevation of the threshold of the central nervous system to convulsive stimuli ...
Succinimide Ethosuximide Caps
N-(e-Maleimidocaproyloxy)succinimide ester (EMCS)
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ICD-10 Diagnosis Code T42.2X5D Adverse effect of succinimides and oxazolidinediones, subs
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Specific Polymers - Catalog - functional peg - ppg - ptmg - peg-ppg maleimide - succinimide - hydroxybenzoic
- T161A High Molecular Weight Polyisobutylene Succinimide,Jinzhou DPF-TH Chemicals Co., Ltd. (global-trade-center.com)
- T161 High Molecular Weight Polyisobutylene Succinimide T161 is a high molecular weight ashless dispersant prepared from highly reactive isobutylene(Mn=2300) by thermal adduction process. (chinacsw.com)
- EMCS crosslinking reagent, N-(e-Maleimidocaproyloxy)succinimide ester, is a non-cleavable, water insoluble, heterobifunctional protein crosslinker that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody. (proteochem.com)
- Ethosuximide is used in the treatment of seizures and belongs to the drug class succinimide anticonvulsants . (drugs.com)
- A study of anticonvulsant activity of N-substituted derivatives of succinimides, thiazolidinediones and their structural congeners. (bvsalud.org)
- A novel biodegradable thermo-responsive poly(aspartic acid) (PAsp) derivatives composed of succinimide and aspartate isopropyl amide were synthesized. (elsevier.com)
- The composition of PAsp derivatives was successfully controlled by changing feed mole ratio of isopropylamine to poly(succinimide) in aminolysis. (elsevier.com)
- Structure-activity relationship study and in vitro biochemical studies with human leukocyte elastase, cathepsin G and proteinase 3 were conducted using a series of succinimide derivatives. (wichita.edu)
- Do not take methsuximide if you are allergic to succinimides (methsuximide or ethosuximide ) or any of the other ingredients in it. (rxwiki.com)
- A succinimide anticonvulsant that increases the seizure threshold. (drugbank.ca)
- In the present study, we computationally investigated the mechanism of succinimide racemization catalyzed by dihydrogen phosphate ion, H 2 PO 4 − , by B3LYP/6-31+G(d,p) density functional theory calculations, using a model compound in which an aminosuccinyl (Asu) residue is capped with acetyl (Ace) and NCH 3 (Nme) groups on the N- and C-termini, respectively (Ace-Asu-Nme). (mdpi.com)
- Two-water-assisted racemization of the succinimide intermediate formed in proteins. (scirp.org)
- In proteins and peptides, d -aspartic acid ( d -Asp) and d -β-Asp residues can be spontaneously formed via racemization of the succinimide intermediate formed from l -Asp and l -asparagine ( l -Asn) residues. (mdpi.com)
- Although nonenzymatic, the succinimide racemization will not occur without a catalyst at room or biological temperature. (mdpi.com)
- In the widely accepted mechanism of the Asp racemization, a succinimide (SI) intermediate is the species which actually undergo the direct racemization. (scirp.org)
- For the laboratory synthesis of NBS, succinimide is dissolved in a solution of sodium hydroxide, ice, and water, then bromine is added, and the N-bromosuccinimide is filtered out as a precipitate. (fsu.edu)
- A mild and efficient synthesis of substituted succinimides by radical β-fragmentation of carbinol amides is described. (csic.es)
- n-HYDROXY SUCCINIMIDE (reagent for peptide synthesis) (1-hydroxy-2,5-pyrrolidinedione) (CAS No.6066-82-6) Laboratory Chemicals Exporters, Lab Chemical Suppliers, Chemical Search, Manufacturers in Mumbai, India. (exporterlabchemicals.com)
- COA information of n-HYDROXY SUCCINIMIDE (reagent for peptide synthesis) (1-hydroxy-2,5-pyrrolidinedione), Alpha Chemika - Exporter of Laboratory Chemical, Lab Chemicals, Laboratory Glassware, Lab Glassware, Laboratory Chemical Supplies and Instruments of standard quality at low price from Mumbai, India. (exporterlabchemicals.com)
- The variation of Hg(OAC) 2 and succinimide (reaction product) has insignificant effect on reaction rate. (hindawi.com)
- Succinimides are also used to form covalent bonds between proteins or peptides and plastics, which is useful in a variety of assay techniques. (wikipedia.org)
- Based on density functional theory calculations using Ace-Asp-Nme (Ace = acetyl, Nme = NHMe) as a model compound, we propose the possibility that acetic acid (AA), which is often used in protein drug formulation for mildly acidic buffer solutions, catalyzes the succinimide formation from Asp residues by acting as a proton-transfer mediator. (mdpi.com)
- The hydrolysis of a succinimide derivative in alkali and neutral media has been studied at the B3LYP/6-31+G* level. (itu.edu.tr)
- Succinimides refers to compounds that contain the succinimide group. (wikipedia.org)
- Succinimides" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
- Detection and quantitation of succinimide in intact protein via hydrazine trapping and chemical derivatization. (harvard.edu)
- Succinimide formation from aspartic acid (Asp) residues is a concern in the formulation of protein drugs. (mdpi.com)
- Succinimide-linked reactions that contribute to protein degradation. (scirp.org)
- DIGITAL.CSIC: Syntesis of subtituted succinimides by radical β-fragmentation of bicyclic carbinol amides. (csic.es)
- Syntesis of subtituted succinimides by radical β-fragmentation of bicyclic carbinol amides. (csic.es)
- Photolysis with visible light in the presence of (diacetoxyiodo)benzene (DIB) and iodine of azabicyclic carbinol amides of the types l-hydroxy-2-azabicyclo[3.3.0]octan-3-on (e.g., 4,6, and 7) and l-hydroxy-2-azabicyclo[3.2.l]octan-3-on (e.g., 16,17, and 21) led to the substituted succinimides in good yields. (csic.es)
- Typical detergents are long-chain amines and amides such as polyisobuteneamine and polyisobuteneamide/succinimide. (wikipedia.org)
- This graph shows the total number of publications written about "Succinimides" by people in Harvard Catalyst Profiles by year, and whether "Succinimides" was a major or minor topic of these publication. (harvard.edu)
- Solution of dextrose (freshly prepared), sodium dodecyl sulfate, triton X-100, and succinimide (all are S D Fine-Chem Limited, Mumbai, India) was prepared with triple distilled water. (hindawi.com)
- A simple organocatalytic system provides efficient access to a series of densely substituted chiral succinimides bearing a quaternary-tertiary carbon stereocenter sequence in good yields, high diastereo- and enantioselectivities through enantioselective conjugate addition of unreactive α-alkyl cyclic ketones to maleimides under microwave-assisted conditions. (rsc.org)
- Succinimide, reagent grade. (cdc.gov)
- In neutral medium, the hydrolysis is facilitated by the presence of a polar continuum, whereas in basic medium, the polar environment hinders hydrolysis of succinimide. (itu.edu.tr)