mRNA Cleavage and Polyadenylation Factors: Factors that are involved in directing the cleavage and POLYADENYLATION of the of MESSENGER RNA near the site of the RNA 3' POLYADENYLATION SIGNALS.Polyadenylation: The addition of a tail of polyadenylic acid (POLY A) to the 3' end of mRNA (RNA, MESSENGER). Polyadenylation involves recognizing the processing site signal, (AAUAAA), and cleaving of the mRNA to create a 3' OH terminal end to which poly A polymerase (POLYNUCLEOTIDE ADENYLYLTRANSFERASE) adds 60-200 adenylate residues. The 3' end processing of some messenger RNAs, such as histone mRNA, is carried out by a different process that does not include the addition of poly A as described here.Cleavage And Polyadenylation Specificity Factor: An RNA-binding protein that recognizes the AAUAAA RNA SEQUENCE at the 3' end of MRNA. It contains four subunits of 30, 73, 100 and 160 kDa molecular size and combines with CLEAVAGE STIMULATION FACTOR to form a stable complex with mRNA that directs the 3' cleavage and polyadenylation reaction.RNA 3' End Processing: The steps that generate the 3' ends of mature RNA molecules. For most mRNAs (RNA, MESSENGER), 3' end processing referred to as POLYADENYLATION includes the addition of POLY A.Polynucleotide Adenylyltransferase: An enzyme that catalyzes the synthesis of polyadenylic acid from ATP. May be due to the action of RNA polymerase (EC 2.7.7.6) or polynucleotide adenylyltransferase (EC 2.7.7.19). EC 2.7.7.19.Poly A: A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Cleavage Stimulation Factor: A RNA-binding protein that stimulates the cleavage of the 3' end of MRNA near the POLYADENYLATION site. It is a heterotrimer of 55-, 64- and 77-kDa subunits and combines with CLEAVAGE STIMULATION FACTOR to form a stable complex with mRNA that directs the 3' cleavage and polyadenylation reaction.RNA Processing, Post-Transcriptional: Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.RNA Precursors: RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.RNA-Binding Proteins: Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.RNA 3' Polyadenylation Signals: Sequences found near the 3' end of MESSENGER RNA that direct the cleavage and addition of multiple ADENINE NUCLEOTIDES to the 3' end of mRNA.Ribonucleoprotein, U7 Small Nuclear: This ribonucleoprotein particle, composed of U7 snRNA, Sm core protein, and U7 snRNP-specific proteins, is involved in the 3'end processing of histone premessenger RNAs.RNA Cleavage: A reaction that severs one of the sugar-phosphate linkages of the phosphodiester backbone of RNA. It is catalyzed enzymatically, chemically, or by radiation. Cleavage may be exonucleolytic, or endonucleolytic.3' Untranslated Regions: The sequence at the 3' end of messenger RNA that does not code for product. This region contains transcription and translation regulating sequences.RNA, Fungal: Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.RNA Polymerase II: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.Argonaute Proteins: A family of RNA-binding proteins that has specificity for MICRORNAS and SMALL INTERFERING RNA molecules. The proteins take part in RNA processing events as core components of RNA-induced silencing complex.RNA Stability: The extent to which an RNA molecule retains its structural integrity and resists degradation by RNASE, and base-catalyzed HYDROLYSIS, under changing in vivo or in vitro conditions.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.RNA-Induced Silencing Complex: A multicomponent, ribonucleoprotein complex comprised of one of the family of ARGONAUTE PROTEINS and the "guide strand" of the one of the 20- to 30-nucleotide small RNAs. RISC cleaves specific RNAs, which are targeted for degradation by homology to these small RNAs. Functions in regulating gene expression are determined by the specific argonaute protein and small RNA including siRNA (RNA, SMALL INTERFERING), miRNA (MICRORNA), or piRNA (PIWI-INTERACTING RNA).Endoribonucleases: A family of enzymes that catalyze the endonucleolytic cleavage of RNA. It includes EC 3.1.26.-, EC 3.1.27.-, EC 3.1.30.-, and EC 3.1.31.-.Codon, Terminator: Any codon that signals the termination of genetic translation (TRANSLATION, GENETIC). PEPTIDE TERMINATION FACTORS bind to the stop codon and trigger the hydrolysis of the aminoacyl bond connecting the completed polypeptide to the tRNA. Terminator codons do not specify amino acids.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.MicroRNAs: Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Protein Subunits: Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.RNA, Plant: Ribonucleic acid in plants having regulatory and catalytic roles as well as involvement in protein synthesis.Ribonucleoproteins: Complexes of RNA-binding proteins with ribonucleic acids (RNA).Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Cytoplasmic Structures: Components of the cytoplasm excluding the CYTOSOL.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Inhibins: Glycoproteins that inhibit pituitary FOLLICLE STIMULATING HORMONE secretion. Inhibins are secreted by the Sertoli cells of the testes, the granulosa cells of the ovarian follicles, the placenta, and other tissues. Inhibins and ACTIVINS are modulators of FOLLICLE STIMULATING HORMONE secretions; both groups belong to the TGF-beta superfamily, as the TRANSFORMING GROWTH FACTOR BETA. Inhibins consist of a disulfide-linked heterodimer with a unique alpha linked to either a beta A or a beta B subunit to form inhibin A or inhibin B, respectivelyGene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Receptors, GABA-A: Cell surface proteins which bind GAMMA-AMINOBUTYRIC ACID and contain an integral membrane chloride channel. Each receptor is assembled as a pentamer from a pool of at least 19 different possible subunits. The receptors belong to a superfamily that share a common CYSTEINE loop.Ribonuclease III: An endoribonuclease that is specific for double-stranded RNA. It plays a role in POST-TRANSCRIPTIONAL RNA PROCESSING of pre-RIBOSOMAL RNA and a variety of other RNA structures that contain double-stranded regions.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Fungal Proteins: Proteins found in any species of fungus.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).RNA Interference: A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Glycoprotein Hormones, alpha Subunit: The alpha chain of pituitary glycoprotein hormones (THYROTROPIN; FOLLICLE STIMULATING HORMONE; LUTEINIZING HORMONE) and the placental CHORIONIC GONADOTROPIN. Within a species, the alpha subunits of these four hormones are identical; the distinct functional characteristics of these glycoprotein hormones are determined by the unique beta subunits. Both subunits, the non-covalently bound heterodimers, are required for full biologic activity.RNA Splicing: The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Follicle Stimulating Hormone, beta Subunit: The beta subunit of follicle stimulating hormone. It is a 15-kDa glycopolypeptide. Full biological activity of FSH requires the non-covalently bound heterodimers of an alpha and a beta subunit. Mutation of the FSHB gene causes delayed puberty, or infertility.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Ribosomes: Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Inhibin-beta Subunits: They are glycopeptides and subunits in INHIBINS and ACTIVINS. Inhibins and activins belong to the transforming growth factor beta superfamily.Kinetics: The rate dynamics in chemical or physical systems.Introns: Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Sodium-Potassium-Exchanging ATPase: An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.RNA, Double-Stranded: RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.Cytoplasm: The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)RNA Probes: RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.DNA Cleavage: A reaction that severs one of the covalent sugar-phosphate linkages between NUCLEOTIDES that compose the sugar phosphate backbone of DNA. It is catalyzed enzymatically, chemically or by radiation. Cleavage may be exonucleolytic - removing the end nucleotide, or endonucleolytic - splitting the strand in two.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Activins: Activins are produced in the pituitary, gonads, and other tissues. By acting locally, they stimulate pituitary FSH secretion and have diverse effects on cell differentiation and embryonic development. Activins are glycoproteins that are hetero- or homodimers of INHIBIN-BETA SUBUNITS.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Arabidopsis Proteins: Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).Alternative Splicing: A process whereby multiple RNA transcripts are generated from a single gene. Alternative splicing involves the splicing together of other possible sets of EXONS during the processing of some, but not all, transcripts of the gene. Thus a particular exon may be connected to any one of several alternative exons to form a mature RNA. The alternative forms of mature MESSENGER RNA produce PROTEIN ISOFORMS in which one part of the isoforms is common while the other parts are different.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Regulatory Sequences, Nucleic Acid: Nucleic acid sequences involved in regulating the expression of genes.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.Ribonucleoprotein, U1 Small Nuclear: A nuclear RNA-protein complex that plays a role in RNA processing. In the nucleoplasm, the U1 snRNP along with other small nuclear ribonucleoproteins (U2, U4-U6, and U5) assemble into SPLICEOSOMES that remove introns from pre-mRNA by splicing. The U1 snRNA forms base pairs with conserved sequence motifs at the 5'-splice site and recognizes both the 5'- and 3'-splice sites and may have a fundamental role in aligning the two sites for the splicing reaction.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Gene Silencing: Interruption or suppression of the expression of a gene at transcriptional or translational levels.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Pituitary Hormones, Anterior: Hormones secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Structurally, they include polypeptide, protein, and glycoprotein molecules.RNA Isoforms: The different gene transcripts generated from a single gene by RNA EDITING or ALTERNATIVE SPLICING of RNA PRECURSORS.Genes, mos: Retrovirus-associated DNA sequences (mos) originally isolated from the Moloney murine sarcoma virus (Mo-MSV). The proto-oncogene mos (c-mos) codes for a protein which is a member of the serine kinase family. There is no evidence as yet that human c-mos can become transformed or has a role in human cancer. However, in mice, activation can occur when the retrovirus-like intracisternal A-particle inserts itself near the c-mos sequence. The human c-mos gene is located at 8q22 on the long arm of chromosome 8.Exoribonucleases: A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Nucleic Acid Precursors: Use for nucleic acid precursors in general or for which there is no specific heading.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Regulatory Sequences, Ribonucleic Acid: Sequences within RNA that regulate the processing, stability (RNA STABILITY) or translation (TRANSLATION, GENETIC) of RNA.Molecular Weight: The sum of the weight of all the atoms in a molecule.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Cleavage Stage, Ovum: The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Gene Expression Regulation, Developmental: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Receptors, Nicotinic: One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors.
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'- ... CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this ... Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene. This gene encodes one of ... "Entrez Gene: CSTF1 cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa". Kleiman, F E; Manley J L (Sep 1999). "Functional ...
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'- ... cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in ... Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene. The protein encoded by ... "Entrez Gene: CSTF3 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa". Takagaki, Y; Manley J L (March 2000). "Complex ...
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'- ... 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO ... Cleavage and polyadenylation specificity factor subunit 3 is a protein that in humans is encoded by the CPSF3 gene. Model ... "Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit". Mol. Cell. Biol ...
... a subunit of the cleavage and polyadenylation specificity factor subunit 1 (CPSF1) complex. This complex polyadenylates the 3' ... FIP1L1 is a Pre-mRNA 3'-end-processing factor. FIP1L1 gene fusions between it and either the platelet-derived growth factor ... 2005). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein ... Pendurthi UR, Alok D, Rao LV (1997). "Binding of factor VIIa to tissue factor induces alterations in gene expression in human ...
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'- ... 1998). "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition ... Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a ... "Entrez Gene: CSTF2 cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa". Takagaki, Y; Manley J L (March 2000). "Complex ...
Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene. Inhibition ... 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO ... The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor ... the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 ...
Murthy KG, Manley JL (Dec 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA ... namely cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factors Im and IIm ... 2000). "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J ... Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene. In most ...
... is a subunit of the cleavage and polyadenylation specificity factor subunit 1 (CPSF1) complex that polyadenylates the 3 ... Factor interacting with PAPOLA and CPSF1 (i.e, FIP1L1; also termed Pre-mRNA 3'-end-processing factor FIP1) is a protein that in ... FIP1L1 is a Pre-mRNA 3'-end-processing factor. FIP1L1 gene fusions between it and either the platelet-derived growth factor ... 2004). "Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo". Mol. Cell. Biol. ...
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation ... "Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit". ... "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". The EMBO ... Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene. ...
Brown, Kirk M; Gilmartin, Gregory M (2003). "A Mechanism for the Regulation of Pre-mRNA 3′ Processing by Human Cleavage Factor ... For example, the expression of CstF-64, a subunit of cleavage stimulatory factor (CstF), increases in macrophages in response ... Cleavage also involves the protein CFII, though it is unknown how. The cleavage site associated with a polyadenylation signal ... "Widespread mRNA polyadenylation events in introns indicate dynamic interplay between polyadenylation and splicing". Genome ...
Murthy, KG; Manley, JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3 ... "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes & ... Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly ... cleavage stimulatory factor and the two poorly understood cleavage factors. The binding of the polynucleotide ...
Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is a enzyme that in humans is encoded by the NUDT21 gene. It ... 2000). "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J ... The protein encoded by this gene is one subunit of the cleavage factor Im complex required for 3' RNA cleavage and ... Kim H, Lee Y (2002). "Interaction of poly(A) polymerase with the 25-kDa subunit of cleavage factor I". Biochem. Biophys. Res. ...
Cleavage stimulation factor 64 kDa subunit, tau variant is a protein that in humans is encoded by the CSTF2T gene. GRCh38: ... Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with ... Pérez Cañadillas JM, Varani G (2003). "Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein". ... "Entrez Gene: CSTF2T cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant". Human CSTF2T genome location and ...
... mRNA) molecule. CstF is recruited by cleavage and polyadenylation specificity factor (CPSF) and assembles into a protein ... "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition". ... Cleavage stimulatory factor or cleavage stimulation factor (CstF or CStF) is a heterotrimeric protein, made up of the proteins ... Cleavage stimulation factor at the US National Library of Medicine Medical Subject Headings (MeSH). ...
Cleavage and polyadenylation specificity factor subunit 7 is a protein that in humans is encoded by the CPSF7 gene. GRCh38: ... "Entrez Gene: FLJ12529 pre-mRNA cleavage factor I, 59 kDa subunit". Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo ... Rüegsegger U, Beyer K, Keller W (1996). "Purification and characterization of human cleavage factor Im involved in the 3' end ...
At the effector domain, it interacts and inhibits cleavage and polyadenylation specificity factor (CPSF30). CPSF30 is part of ... It prevents polyadenylation of cellular mRNAs to circumvent antiviral responses of the host, e.g., maturation and translation ... This kinase phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (elF-2 alpha), leading to a decrease ... NS1 might also inhibit splicing of pre-mRNA by binding to a stem-bulge region in U6 small nuclear RNA (snRNA). In addition, NS1 ...
2 α subunits, 1 β subunit, 1 β' subunit, and 1 ω subunit. In bacteria, there is one general RNA transcription factor: sigma. ... If the gene encodes a protein, the transcription produces messenger RNA (mRNA); the mRNA, in turn, serves as a template for the ... This may include polyadenylation, capping, and splicing. The RNA may remain in the nucleus or exit to the cytoplasm through the ... Transcription termination in eukaryotes is less well understood than in bacteria, but involves cleavage of the new transcript ...
2 α subunits, 1 β subunit, 1 β' subunit, and 1 ω subunit. In bacteria, there is one general RNA transcription factor: sigma. ... Precursor mRNA (pre-mRNA / hnRNA). *5' capping. *Splicing. *Polyadenylation. *Histone acetylation and deacetylation ... Transcription termination in eukaryotes is less well understood than in bacteria, but involves cleavage of the new transcript ... If the gene encodes a protein, the transcription produces messenger RNA (mRNA); the mRNA, in turn, serves as a template for the ...
TFIIF (two subunits, RAP30 and RAP74, showing some similarity to bacterial sigma factors) and Pol II enter the complex together ... end cleavage, and polyadenylation. Ser5 phosphorylation (Ser5PO4) near the 5′ ends of genes depends principally on the kinase ... The PCTD (phosphoCTD of an RNAPII0) physically links pre-mRNA processing to transcription by tethering processing factors to ... General transcription factors (GTFs) or basal transcription factors are protein transcription factors that have been shown to ...
... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... Messenger RNA (mRNA)-template for the synthesis of proteins by ribosomes.. *Non-coding RNA or "RNA genes"-a broad class of ... Transcription termination in eukaryotes is less well understood than in bacteria, but involves cleavage of the new transcript ... followed by template-independent addition of adenines at its new 3' end, in a process called polyadenylation.[21] ...
Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Nucleic ... a member of the mRNA export complex: a novel link between mRNA export machinery and signal transduction pathways in cell ... THO complex subunit 5 homolog is a protein that in humans is encoded by the THOC5 gene.THOC5 is a member of THO complex which ... a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and ...
2001). "hnRNP F influences binding of a 64-kilodalton subunit of cleavage stimulation factor to mRNA precursors in mouse B ... polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some ... Honoré B, Vorum H, Baandrup U (1999). "hnRNPs H, H' and F behave differently with respect to posttranslational cleavage and ... 2001). "Evidence that dim1 associates with proteins involved in pre-mRNA splicing, and delineation of residues essential for ...
... and a polyadenylation signal in exon 4 causes cleavage of the mRNA at that point. The resulting mRNA is a transcriptional ... on the pre-mRNA. However, as part of the complexity of alternative splicing, it is noted that the effects of a splicing factor ... A spliceosome assembles on an intron, and the snRNP subunits fold the RNA so that the 5' and 3' ends of the intron are joined. ... and terminates after a polyadenylation site in exon 4. Another mRNA is produced from this pre-mRNA by skipping exon 4, and ...
The three main modifications are 5' capping, 3' polyadenylation, and RNA splicing. While in the nucleus, pre-mRNA is associated ... as well as a large variety of transcription factors that regulate expression. Newly synthesized mRNA molecules are known as ... Most of the cleavage and modification of rRNAs occurs in the DFC, while the latter steps involving protein assembly onto the ... Most proteins, ribosomal subunits, and some DNAs are transported through the pore complexes in a process mediated by a family ...
Polyadenylation is also important for transcription termination, export of the mRNA from the nucleus, and translation. mRNA can ... such as β-actin mRNA. Upon export from the nucleus, actin mRNA associates with ZBP1 and the 40S subunit. The complex is bound ... After the mRNA has been cleaved, around 250 adenosine residues are added to the free 3' end at the cleavage site. This reaction ... "Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping" (PDF), Mol. Cell, 20 (6): 905-15, doi ...
Polyadenylation is also important for transcription termination, export of the mRNA from the nucleus, and translation. mRNA can ... such as β-actin mRNA.[9] Upon export from the nucleus, actin mRNA associates with ZBP1 and the 40S subunit. The complex is ... After the mRNA has been cleaved, around 250 adenosine residues are added to the free 3' end at the cleavage site. This reaction ... "Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping" (PDF), Mol. Cell, 20 (6): 905-15, doi ...
Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. CFIm is ... Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3-processing. Binds to cleavage and ... Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation.. ... Tandem affinity purification and mass spectrometry analysis identify 59kDa cleavage and polyadenylation specific factor 7 ( ...
CSTF 50 kDa subunit; CF-1 50 kDa subunit; CstF-50 \ 10-288-22329F for more molecular products just contact us ... Cleavage and polyadenylation specificity factor 68 kDa subunit) (CFIm68) (CPSF 68 kDa subunit) (Pre-mRNA cleavage factor Im 68 ... Cleavage and polyadenylation specificity factor 59 kDa subunit) (CFIm59) (CPSF 59 kDa subunit) (Pre-mRNA cleavage factor Im 59 ... Cleavage and polyadenylation specificity factor subunit 5 (Cleavage and polyadenylation specificity factor 25 kDa subunit) ( ...
... hnRNP F influences binding of a 64-kilodalton subunit of cleavage stimulation factor to mRNA precursors in mouse B cells. Mol ... gene that encodes the probable ortholog of the 30-kD subunit of the mammalian cleavage and polyadenylation specificity factor ( ... The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc ... is a gene that encodes a protein similar to the 30-kD subunit of the mammalian cleavage and polyadenylation specificity factor ...
Tyr1 phosphorylation levels rise downstream of the transcription start site (TSS), and decrease before the polyadenylation (pA ... Tyr1 phosphorylation blocks CTD binding by these termination factors, but stimulates binding of elongation factor Spt6. These ... Tyr1-phosphorylated gene bodies are depleted of CTD-binding termination factors Nrd1, Pcf11, and Rtt103. ... Pol II recruits different factors via its CTD, which consists of heptapeptide repeats with the sequence Tyr1-Ser2-Pro3-Thr4- ...
p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of ... Cleavage and polyadenylation specif.... Cleavage and polyadenylation specificity factor subunit 1 (Cleavage and polyadenylation ... Cleavage and polyadenylation-specificity factor subunit 1Imported. ,p>Information which has been imported from another database ... tr,E9PIM1,E9PIM1_HUMAN Cleavage and polyadenylation-specificity factor subunit 1 OS=Homo sapiens OX=9606 GN=CPSF1 PE=1 SV=3 ...
Murthy, KG; Manley, JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3 ... "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3-end formation". Genes & ... Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3 signaling region from a newly ... cleavage stimulatory factor and the two poorly understood cleavage factors. The binding of the polynucleotide ...
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3- ... cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in ... Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene. The protein encoded by ... "Entrez Gene: CSTF3 cleavage stimulation factor, 3 pre-RNA, subunit 3, 77kDa". Takagaki, Y; Manley J L (March 2000). "Complex ...
CSTF is involved in the polyadenylation and 3end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this ... cleavage stimulation factor subunit 1. Names. CF-1 50 kDa subunit. CSTF 50 kDa subunit. cleavage stimulation factor 50 kDa ... CSTF1 cleavage stimulation factor subunit 1 [Homo sapiens] CSTF1 cleavage stimulation factor subunit 1 [Homo sapiens]. Gene ID: ... cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kD. cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kDa. ...
Cleavage And Polyadenylation Factor I Subunit 1, including: function, proteins, disorders, pathways, orthologs, and expression ... Cleavage And Polyadenylation Factor I Subunit 1 2 3 5 * Pre-MRNA Cleavage Complex II Protein Clp1 3 4 ... Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors. (PMID: ... CLP1 (Cleavage And Polyadenylation Factor I Subunit 1) is a Protein Coding gene. Diseases associated with CLP1 include ...
Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK. ... mRNA cleavage and polyadenylation specificity factor complex Source: GO_Central. *nucleoplasm Source: HPA ... AF161530 mRNA Translation: AAF29145.1. AF277178 mRNA Translation: AAK07538.1. AJ276409 mRNA Translation: CAC81713.1. AK001809 ... AF161530 mRNA Translation: AAF29145.1. AF277178 mRNA Translation: AAK07538.1. AJ276409 mRNA Translation: CAC81713.1. AK001809 ...
Pre-mRNA binding subunit of the mRNA cleavage and polyadenylation specificity factor complex; involved in mRNA polyadenylation ... Essential conserved subunit of CPF cleavage and polyadenylation factor; plays a role in 3 end formation of mRNA via the ... pre-mRNA cleavage required for polyadenylation, and protein ubiquitination; localizes to both nucleus and cytosol. View ... specific cleavage and polyadenylation of pre-mRNA; contains a ubiquitin-like (UBL) domain, a RNA-binding zinc knuckle motif and ...
Crystal structure of an archaeal cleavage and polyadenylation specificity factor subunit from Pyrococcus horikoshii.. Proteins ... Polyadenylation factor CPSF-73 is the pre-mRNA 3-end-processing endonuclease.. Nature 444 953-6 2006 ... Crystal structure of an archaeal cleavage and polyadenylation specificity factor subunit from Pyrococcus horikoshii.. Proteins ... Polyadenylation factor CPSF-73 is the pre-mRNA 3-end-processing endonuclease.. Nature 444 953-6 2006 ...
The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor ... end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular ... cleavage and polyadenylation specific factor 4, 30kDa Assay Type: Probe Assay Design: Exonic Application: Gene Expression ... cleavage and polyadenylation specific factor 4, 30kDa Assay Type: SYBR® Green Assay Design: Exonic Application: Gene Expression ...
Cleavage and polyadenylation specificity factor subunit 3; CPSF; CPSF 73 kDa subunit; CPSF-73; CPSF73; mRNA 3-end-processing ... mRNA polyadenylation mRNA cleavage mRNA 3-end processing by stem-loop binding and cleavage mRNA export from nucleus mRNA 3- ... cleavage and polyadenylation specificity factor 3; Cleavage and polyadenylation specificity factor 73 kDa subunit; ... CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end- ...
Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.. Mol. Cell, Year 2019. Chris H ... Activation of the Endonuclease that Defines mRNA 3 ...
Stiff matrix instigates type I collagen biogenesis by mammalian cleavage factor I complex-mediated alternative polyadenylation ... Effects of knockdown/overexpression of specific CFIm subunits on matrix stiffness-regulated APA and expression of COL1A1 and ... These data suggest that stiff matrix instigates type I collagen biogenesis by selectively targeting mRNA transcripts for 3′ UTR ... Stiff matrix instigates type I collagen biogenesis by mammalian cleavage factor I complex-mediated alternative polyadenylation ...
... kilodalton subunit of the CstF polyadenylation factor binds to pre-mRNA downstream of the cleavage site and influences cleavage ... 1995) The 160kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3′ end formation. Genes Dev. ... tail onto the cleaved mRNA. Cleavage and polyadenylation specificity factor is required for both the cleavage and the poly(A) ... 1991) Purification of the cleavage and polyadenylation factor involved in 3′ processing of mRNA precursors. J. Biol. Chem. 266: ...
Cleavage And Polyadenylation Specific Factor 6, including: function, proteins, disorders, pathways, orthologs, and expression. ... Cleavage And Polyadenylation Specificity Factor Subunit 6 3 * Pre-MRNA Cleavage Factor I, 68kD Subunit 3 ... Subnuclear localization and dynamics of the Pre-mRNA 3 end processing factor mammalian cleavage factor I 68-kDa subunit. (PMID ... The protein encoded by this gene is one subunit of a cleavage factor required for 3 RNA cleavage and polyadenylation ...
Pre mRNA cleavage factor Im 59 kDa subunit. *Pre-mRNA cleavage factor Im 59 kDa subunit ... that plays a key role in pre-mRNA 3 processing. Binds to cleavage and polyadenylation RNA substrates. ... Component of the cleavage factor Im complex (CFIm) ... Cleavage and polyadenylation specificity factor 59 kDa subunit ... Cleavage and polyadenylation specificity factor 7. *Cleavage and polyadenylation specificity factor subunit 7 ...
1995). The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3′-end formation. Genes Dev ... Of these subunits, one of the more enigmatic is the 30-kD subunit of the Cleavage and Polyadenylation Stimulatory Factor ( ... 1997). The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding ... The Arabidopsis thaliana ortholog of the 30-kD subunit of the mammalian Cleavage and Polyadenylation Specificity Factor (CPSF30 ...
The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3′-end formation. Genes Dev. 9, ... Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation ... Separation of factors required for cleavage and polyadenylation of yeast pre-mRNA. Mol. Cell. Biol. 12, 3470-3481 (1992). doi: ... The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc ...
CL-P1; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); COLEC12; EC 2.7.1.78; hClp1; HEAB; HEABATP ... Pre-mRNA cleavage complex II protein Clp1; SCARA4; SRCL Type I ... binding protein; homolog of yeast CFIA subunit Clp1p; NSR2; ...
Cleavage stimulation factor subunit 1 (CSTF1) is involved in the polyadenylation and 3end cleavage of pre-mRNAs. The CSTF1 ... Cleavage stimulation factor subunit 1, CF-1 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit, CSTF 50 kDa subunit, ... CSTF1 is one of the numerous factors necessary for polyadenylation and 3-end cleavage of mammalian pre-mRNAs. CSTF1 is ... gene encodes one of three subunits which merge to produce cleavage stimulation factor (CSTF). Like the mammalian G protein beta ...
Cleavage stimulation factor subunit 3 (CSTF3 or CstF-77) and crooked neck-like protein 1 (CRNKL1) from mammals. CSTF3 is ... required for polyadenylation and 3-end cleavage of mammalian pre-mRNAs [(PUBMED:23874216)]. ... Prp6 and Prp39 are involved in pre-mRNA splicing. ...
... mRNA ... The protein encoded by this gene is one subunit of a cleavage factor required for 3 RNA cleavage and polyadenylation ... mRNA cleavage and polyadenylation specificity factor complex • cleavage body Biological process • mRNA splicing, via ... end cleavage and polyadenylation of pre-mRNAs .... Cleavage Enhancement. ... Cleavage enhancement encompasses a range of ... Cleavage. Cleavage may refer to:. *Cleavage (breasts), partial exposure of the separation between a womans breasts. *Cleavage ...
  • In different phases of the transcription cycle, Pol II recruits different factors via its CTD, which consists of heptapeptide repeats with the sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. (omicsdi.org)
  • Data provide evidence that CFIm exists as a heterotetramer of 25-kD, 59-kD and 68-kD subunits of CFIm: CFIm25, CFIm59 and CFIm68. (nih.gov)
more