Hydroxyl Radical: The univalent radical OH. Hydroxyl radical is a potent oxidizing agent.Free Radicals: Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.Hydroxides: Inorganic compounds that contain the OH- group.Free Radical Scavengers: Substances that influence the course of a chemical reaction by ready combination with free radicals. Among other effects, this combining activity protects pancreatic islets against damage by cytokines and prevents myocardial and pulmonary perfusion injuries.Hydrogen Peroxide: A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.Electron Spin Resonance Spectroscopy: A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.DeoxyriboseProtein Footprinting: A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Superoxides: Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides.Deferoxamine: Natural product isolated from Streptomyces pilosus. It forms iron complexes and is used as a chelating agent, particularly in the mesylate form.Benzoic Acid: A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Thiourea: A photographic fixative used also in the manufacture of resins. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), this substance may reasonably be anticipated to be a carcinogen (Merck Index, 9th ed). Many of its derivatives are ANTITHYROID AGENTS and/or FREE RADICAL SCAVENGERS.Xanthine Oxidase: An iron-molybdenum flavoprotein containing FLAVIN-ADENINE DINUCLEOTIDE that oxidizes hypoxanthine, some other purines and pterins, and aldehydes. Deficiency of the enzyme, an autosomal recessive trait, causes xanthinuria.Catalase: An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.Ferrous Compounds: Inorganic or organic compounds that contain divalent iron.Superoxide Dismutase: An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 188.8.131.52.Cyclic N-Oxides: Heterocyclic compounds in which an oxygen is attached to a cyclic nitrogen.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Antioxidants: Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.Dimethyl Sulfoxide: A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.Gentisates: Salts and esters of gentisic acid.Spin Labels: Molecules which contain an atom or a group of atoms exhibiting an unpaired electron spin that can be detected by electron spin resonance spectroscopy and can be bonded to another molecule. (McGraw-Hill Dictionary of Chemical and Technical Terms, 4th ed)Reactive Oxygen Species: Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.PicratesMannitol: A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.Ferric Compounds: Inorganic or organic compounds containing trivalent iron.Hypochlorous Acid: An oxyacid of chlorine (HClO) containing monovalent chlorine that acts as an oxidizing or reducing agent.Spin Trapping: A technique for detecting short-lived reactive FREE RADICALS in biological systems by providing a nitrone or nitrose compound for an addition reaction to occur which produces an ELECTRON SPIN RESONANCE SPECTROSCOPY-detectable aminoxyl radical. In spin trapping, the compound trapping the radical is called the spin trap and the addition product of the radical is identified as the spin adduct. (Free Rad Res Comm 1990;9(3-6):163)Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Iron Chelating Agents: Organic chemicals that form two or more coordination links with an iron ion. Once coordination has occurred, the complex formed is called a chelate. The iron-binding porphyrin group of hemoglobin is an example of a metal chelate found in biological systems.Pulse Radiolysis: Use of a pulse of X-rays or fast electrons to generate free radicals for spectroscopic examination.Oxidants: Electron-accepting molecules in chemical reactions in which electrons are transferred from one molecule to another (OXIDATION-REDUCTION).Nitrogen Oxides: Inorganic oxides that contain nitrogen.Lipid Peroxidation: Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.Hydroxylation: Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)Edetic Acid: A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.Copper: A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).2,2'-Dipyridyl: A reagent used for the determination of iron.Ascorbic Acid: A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.Salicylates: The salts or esters of salicylic acids, or salicylate esters of an organic acid. Some of these have analgesic, antipyretic, and anti-inflammatory activities by inhibiting prostaglandin synthesis.Lipid Peroxides: Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.Xanthine: A purine base found in most body tissues and fluids, certain plants, and some urinary calculi. It is an intermediate in the degradation of adenosine monophosphate to uric acid, being formed by oxidation of hypoxanthine. The methylated xanthine compounds caffeine, theobromine, and theophylline and their derivatives are used in medicine for their bronchodilator effects. (Dorland, 28th ed)Kinetics: The rate dynamics in chemical or physical systems.Singlet Oxygen: An excited state of molecular oxygen generated photochemically or chemically. Singlet oxygen reacts with a variety of biological molecules such as NUCLEIC ACIDS; PROTEINS; and LIPIDS; causing oxidative damages.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Chelating Agents: Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS.Benzoates: Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.Deoxyguanosine: A nucleoside consisting of the base guanine and the sugar deoxyribose.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Peroxides: A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)Pyrogallol: A trihydroxybenzene or dihydroxy phenol that can be prepared by heating GALLIC ACID.Plant Extracts: Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Phenols: Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Environmental Remediation: Removal of ENVIRONMENTAL POLLUTANTS or contaminants for the general protection of the environment. This is accomplished by various chemical, biological, and bulk movement methods, in conjunction with ENVIRONMENTAL MONITORING.Biphenyl CompoundsProstatectomy: Complete or partial surgical removal of the prostate. Three primary approaches are commonly employed: suprapubic - removal through an incision above the pubis and through the urinary bladder; retropubic - as for suprapubic but without entering the urinary bladder; and transurethral (TRANSURETHRAL RESECTION OF PROSTATE).Glutathione: A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Methylurea Compounds: Urea compounds which are substituted with one or more methyl groups.Peroxidase: A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 184.108.40.206.DNA Footprinting: A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Pholiota: A genus of basidiomycetous mushroom in the family Strophariaceae, exhibiting a bipolar mating system.PhenanthrolinesMolecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Luminescent Measurements: Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.Pentetic Acid: An iron chelating agent with properties like EDETIC ACID. DTPA has also been used as a chelator for other metals, such as plutonium.AnserinePhotolysis: Chemical bond cleavage reactions resulting from absorption of radiant energy.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Malondialdehyde: The dialdehyde of malonic acid.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Electrolysis: Destruction by passage of a galvanic electric current, as in disintegration of a chemical compound in solution.Hypoxanthine: A purine and a reaction intermediate in the metabolism of adenosine and in the formation of nucleic acids by the salvage pathway.Thiobarbituric Acid Reactive Substances: Low-molecular-weight end products, probably malondialdehyde, that are formed during the decomposition of lipid peroxidation products. These compounds react with thiobarbituric acid to form a fluorescent red adduct.Nitrates: Inorganic or organic salts and esters of nitric acid. These compounds contain the NO3- radical.Citrullus: A plant genus of the family CUCURBITACEAE known for the edible fruit.Lactoferrin: An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Sulfonic Acids: Inorganic or organic oxy acids of sulfur which contain the RSO2(OH) radical.Hypoxanthines: Purine bases related to hypoxanthine, an intermediate product of uric acid synthesis and a breakdown product of adenine catabolism.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.PeroxidasesDiuretics, Osmotic: Compounds that increase urine volume by increasing the amount of osmotically active solute in the urine. Osmotic diuretics also increase the osmolarity of plasma.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Tiopronin: Sulfhydryl acylated derivative of GLYCINE.Benserazide: An inhibitor of DOPA DECARBOXYLASE that does not enter the central nervous system. It is often given with LEVODOPA in the treatment of parkinsonism to prevent the conversion of levodopa to dopamine in the periphery, thereby increasing the amount that reaches the central nervous system and reducing the required dose. It has no antiparkinson actions when given alone.Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 220.127.116.11.Tyrosine: A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.Paraquat: A poisonous dipyridilium compound used as contact herbicide. Contact with concentrated solutions causes irritation of the skin, cracking and shedding of the nails, and delayed healing of cuts and wounds.Chemistry: A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.HydroquinonesThiobarbiturates: Compounds in which one or more of the ketone groups on the pyrimidine ring of barbituric acid are replaced by thione groups.Naphthacenes: Polyacenes with four ortho-fused benzene rings in a straight linear arrangement. This group is best known for the subclass called TETRACYCLINES.Synchrotrons: Devices for accelerating protons or electrons in closed orbits where the accelerating voltage and magnetic field strength varies (the accelerating voltage is held constant for electrons) in order to keep the orbit radius constant.ZymosanMolsidomine: A morpholinyl sydnone imine ethyl ester, having a nitrogen in place of the keto oxygen. It acts as NITRIC OXIDE DONORS and is a vasodilator that has been used in ANGINA PECTORIS.Aldehydes: Organic compounds containing a carbonyl group in the form -CHO.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
Shafer GE, Price MA, Tullius TD (1989). "Use of the hydroxyl radical and gel electrophoresis to study DNA structure". ... Other studies have extended the method to study early onset protein damage given the radical basis of the method and the ... Time-resolved hydroxyl radical protein footprinting employing mass spectrometry analysis was developed in the late 1990s in ... A critical feature of these experiments is the need to expose proteins to hydroxyl radicals for limited timescales on the order ...
"On the Electrophilicity of Hydroxyl Radical: A Laser Flash Photolysis and Computational Study". Journal of the American ... Pyrithione can be used as a source of hydroxyl radical in organic synthesis as it photochemically decomposes to HO• and ( ... The mechanisms by which this work are the subject of ongoing study. It can be used as an antibacterial agent against ... pyridin-2-yl)sulfanyl radical. The conjugate base of pyrithione is an anion containing two donor atoms, a sulfur atom and an ...
Monohydrogen chalcogenide compounds, if unstable, do exist; the radical compounds hydroxyl (HO) and hydroperoxyl (HO2) being ... Nishimoto, Akiko; Zhang, Daisy Y. (2003). "Hypervalency in sulfur? Ab initio and DFT studies of the structures of thiosulfate ... The respective sulfur analogue for hydroxyl is sulfanyl (HS). One or both of the protium atoms in water can be substituted with ... A Mechanistic Pulse Radiolysis Study". J. Phys. Chem. A. 104 (17): 4011-4016. Bibcode:2000JPCA..104.4011G. doi:10.1021/ ...
Protein mass spectrometry
In this technique, solvent-exposed sites of the protein are modified by hydroxyl radicals. Its combination with rapid mixing ... The application of mass spectrometry to study proteins became popularized in the 1980s after the development of MALDI and ESI. ... The top-down approach however is mostly limited to low-throughput single-protein studies due to issues involved in handling ... Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an ...
Radical reactions of NAA was studied by using pulse radiolysis technique. Hydroxyl adduct radical was formed as the ... NAA present in the environment undergoes oxidation reactions with hydroxyl radicals and sulphate radicals. ... Radical chemistry of glucosamine naphthalene acetic acid and naphthalene acetic acid: a pulse radiolysis study. J. Phys. Org. ... intermediate during the reaction of hydroxyl radical with NAA. The intermediate Naphtyl methyl radical was formed during the ...
The curcuminoids are capable of inhibiting damage to super coiled plasmid DNA by hydroxyl radicals. It was concluded that the ... In vitro studies show a prolonged release of curcuminoids from the nanoparticle preparate up to 12 hours and the curcuminoids ... But there are some stability issues which have not been overcome yet, further studies need to be done to find a suitable ... Tomren MA, Másson M, Loftsson T, Tønnesen HH (June 2007). "Studies on curcumin and curcuminoids XXXI. Symmetric and asymmetric ...
Indirect photooxidation in the atmosphere through reaction with hydroxyl radicals was estimated. Despite limited data, the ... Study XBL 91071. XenoBiotic Laboratories, Inc., USA, unpublished) cited in There is a data gap on presence or formation of ... In a study of diphenylamine metabolism in harvested and dipped apples at different time intervals it was observed that ... The Acceptable Daily Intake of diphenylamine was 0.075 mg/kg bw/day based on the 2-year rat study, applying a safety factor of ...
... based on how readily hydrogen peroxide can decompose into a hydroxyl radical and how superoxide radical byproducts of cellular ... At least one study has also tried to link hydrogen peroxide production to cancer. These studies have frequently been quoted ... It is then quickly dissociated to OH· radicals. The radicals serve as initiator of radical polymerization, which surrounds the ... In advanced oxidation processing, the Fenton reaction gives the highly reactive hydroxyl radical (·OH). This degrades ...
... and the hydroxyl radical have worked in lowering levels of anatoxin-a. Optimizing the treatment process would involve the ... Studies have shown that some microorganisms are capable of degrading anatoxin-a. A study done by Kiviranta and colleagues in ... Others have called for additional studies to determine more about how to use activated carbon effectively. More common methods ... Anatoxin-a is a very powerful nicotinic acetylcholine receptor agonist and as such has been extensively studied for medicinal ...
They found that beer staling is reduced by scavengers of the hydroxyl radical, such as mannitol and ascorbic acid. They also ... A study done by Heuberger et. al. concludes that storage temperature of beers affects the flavor stability. They found that the ... In another study done by Vanderhaegen et. al., different aging conditions were tested on a bottled beer after 6 months. They ... A study done by Charles Bamforth and Roy Parsons also confirms that beer staling flavors are caused by various carbonyl ...
Identification of carboxyl, peroxyl, hydroxyl and alkoxyl radicals in rest rats give plausibility to the involvement of an ... Past animal studies have shown good correlations between organic peroxide damage in human case reports and test animals. 28-day ... 28 Day oral rat repeat dose studies with organic peroxides have shown alterations in rat kidneys in the form of histopathologic ... Direct detection of radical generation in rat liver nuclei on treatment with tumour-promoting hydroperoxides and related ...
Oxidative stress can be caused by hydroxyl free radicals and hydrogen peroxide, both of which are byproducts of XO activity. ... A study found that patients with chronic obstructive pulmonary disease (COPD) had a decrease in oxidative stress, including ... XO has also been found to produce the strong one-electron oxidant carbonate radical anion from oxidation with acetaldehyde in ... It was suggested that the carbonate radical was likely produced in one of the enzyme's redox centers with a peroxymonocarbonate ...
Cadet, J., Delatour, T., Douki, T., Gasparutto, D., Pouget, J., Ravanat, J., & Sauvaigo, S. (1999). Hydroxyl radicals and DNA ... In most studies, NADH is believed to be the preferred electron donor, although reports have shown that NAPDH is equally used as ... Release of iron can undergo Fenton reaction with hydrogen peroxide to yield hydroxyl radicals, which damage any biological ... In one study, apocynin showed clinical benefits in the treatment of influenza as it decreased influenza-induced lung ...
Saito, T., Miyabe, Y., Ide, H., Yamamoto, O., Hydroxyl radical scavenging ability of bacterioruberin, Radiat Phys Chem, 1997, ... Methods for gene replacement and systematic knockout have been developed, so H. salinarum is an ideal candidate for the study ... The bacterioruberin radical produced is less reactive than the initial radical, and will likely react with another radical, ... Ide, H., Takeshi, S., Hiroaki, T., Studies on the antioxidation activity of bacterioruberin, Urakami Found Mem, 1998, 6:127-33 ...
... and hydroxyl radical and nuclease footprinting studies. In the 1990s, crystallography and nucleic acid NMR methods became ... While most studies have focused on the identities of the four bases nearest to the junction on each arm, it is evident that ... The first experimental evidence for the structure of the Holliday junction came from electron microscopy studies in the late ... allowing for more direct study of their physical properties. Much of the early analysis of Holliday junction structure was ...
... is not persistent in air, as it is rapidly degraded by hydroxyl radicals. Nonylphenol is considered to be an ... Another study calculated a daily intake for the more exposed group of infants in the range of 0.23-0.65 µg/ kg bodyweight/ day ... One study conducted in Italian women showed that nonylphenol was one of the highest contaminants at a concentration of 32 ng/mL ... The study also found a positive correlation between fish consumption and the concentration of nonylphenol in breast milk. This ...
... it has been observed that TPZ primarily produces hydroxyl or and benzotriazinyl radicals as the DNA damaging reactive species. ... This study will help to optimize the safe tollerable dose of TPZ, when it is administered with embolization in liver cancer. ... "Dose-defining Study of Tirapazamine Combined With Embolization in Liver Cancer". clinicaltrials.gov. Mason, J. C.; Tennant, G ... Tirapazamine (SR-4233) is an experimental anticancer drug that is activated to a toxic radical only at very low levels of ...
showed that a substantial portion of their bactericidal activity results from release of the hydroxyl radical and induction of ... In 2011 studies of Legionella pneumophila, Charpentier et al. tested 64 toxic molecules to determine which ones induce ... Although these aminoglycoside antibiotics were previously regarded as non-DNA damaging, recent studies in 2012 of Foti et al. ... Since then, natural competence has been studied in a number of different bacteria, particularly Bacillus subtilis, ...
The 3'-hydroxyl group of pyridoxamine allows for efficient hydroxyl radical scavenging. Pyridoxamine inhibits the Maillard ... One research study found that pyridoxamine specifically reacts with the carbonyl group in Amadori products, but inhibition of ... In one study, pyridoxamine was more effective at protecting from ionizing radiation-induced gastrointestinal epithelial ... Chemically it is based on a pyridine ring structure, with hydroxyl, methyl, aminomethyl, and hydroxymethyl substituents. It ...
Advanced oxidation process
Hydroxyl radicals are produced with the help of one or more primary oxidants (e.g. ozone, hydrogen peroxide, oxygen) and/or ... In fact, there has been some studies that offer constructive solutions. For instance, doping TiO2 with non-metallic elements ... AOPs rely on in-situ production of highly reactive hydroxyl radicals (·OH). These reactive species are the strongest oxidants ... As a result of their very nature, AOPs require hydroxyl radicals and other reagents proportional to the quantity of ...
At low concentrations, its acts as a super-oxide scavenger, specifically against the highly reactive hydroxyl radical and ... A cohort study in men showed that consumption of flavonoid containing food decreased the risk of mortality from coronary heart ... Many studies suggest that consuming kaempferol may reduce the risk of various cancers, and it is currently under consideration ... In vitro studies along with some animal testing has demonstrated the wide range of potential anti-cancer properties of ...
For example, water dissociates under alpha radiation into a hydrogen radical and a hydroxyl radical, unlike ionization of water ... Of all the radiation-chemical reactions that have been studied, the most important is the decomposition of water. When exposed ... It is believed that the enhanced concentration of hydroxyl present in irradiated water in the inner coolant loops of a light- ... photolysis of the Cl2 molecule into two Cl-radicals, where (ultraviolet or visible) light is used. ...
... hydroxyl radical, and secondary ultrafine particles. Many air fresheners employ carcinogens, volatile organic compounds and ... A study in 2006 found that the prominent products of the reaction of terpenes found in air fresheners with ozone included ... via Avon Longitudinal Study of Parents and Children Key Findings Steinemann, A.C. (2009). "Fragranced consumer products and ... In 2008, Anne C. Steinemann of the University of Washington published a study of top-selling air fresheners and laundry ...
This catalyzes the production of very reactive radical ions, such as hydroxyl radical in a manner similar to Fenton chemistry. ... One study of two strains of mice fed copper compounds found a varying increased incidence of reticulum cell sarcoma in males of ... Free Radical Biology and Medicine. Elsevier, Free Radical Biology and Medicine 1999. 27: 1114-1121. doi:10.1016/S0891-5849(99) ... This increase in unmediated reactive radicals is generally termed oxidative stress, and is an active area of research in a ...
This occurs as highly reactive hydroxyl radicals target DNA. DNA degradation has a direct effect on plant germination, growth ... A 2009 study noted significant decreases in vitamins A, C and K as well as folic acid and thiamin can occur in as little as one ... However, a 2014 study suggested that plants were able to germinate and survive a period of 50 days on a Martian and lunar soil ... However, a 2006 study suggests maintaining elevated CO2 concentrations can mitigate the effects of hypobaric conditions as low ...
Poeggeler B, Reiter RJ, Tan DX, Chen LD, Manchester LC (May 1993). "Melatonin, hydroxyl radical-mediated oxidative damage, and ... However, many studies on melatonin and autism rely on self-reported levels of improvement and more rigorous research is needed ... especially the hydroxyl radical that attacks DNA, proteins, and cellular membranes. Melatonin has been described as a broadly ... endogenous hydroxyl radical scavenger". Endocrine J. 1: 57-60. Reiter RJ, Manchester LC, Tan DX (September 2010). "Neurotoxins ...
Experimental and theoretical studies on aqueous-phase reactivity of hydroxyl radicals with multiple carboxylated and...
... we shed light on the initial addition of hydroxyl radicals (HO˙) to multiple carboxylated and hydroxylated benzene compounds in ... In this study, we shed light on the initial addition of hydroxyl radicals (HO˙) to multiple carboxylated and hydroxylated ... Experimental and theoretical studies on aqueous-phase reactivity of hydroxyl radicals with multiple carboxylated and ... Experimental and theoretical studies on aqueous-phase reactivity of hydroxyl radicals with multiple carboxylated and ...
Production of cold formaldehyde molecules for study and control of chemical reaction dynamics with hydroxyl radicals | JILA...
Production of cold formaldehyde molecules for study and control of chemical reaction dynamics with hydroxyl radicals. Title. ... Production of cold formaldehyde molecules for study and control of chemical reaction dynamics with hydroxyl radicals. ... Production of cold formaldehyde molecules for study and control of chemical reaction dynamics with hydroxyl radicals ...
ESR Study of Hydroxyl Radicals, Superoxide Anion Radicals and Molecular Singlet Oxygen Generated by Mercaptoundecahydro- closo...
... we have reported the generation of superoxide anion-radicals by neutron irradiation and UV light irradiation of mercaptoborates ... ESR Study of Hydroxyl Radicals, Superoxide Anion Radicals and Molecular Singlet Oxygen Generated by Mercaptoundecahydro-closo- ... Stopka P., Burian J., Štrouf O. (1996) ESR Study of Hydroxyl Radicals, Superoxide Anion Radicals and Molecular Singlet Oxygen ... The generation of hydroxyl radicals ·OH, superoxide anion-radicals O2·−, and singlet molecular oxygen 1O2 were identified and ...
Studies of the reactions of hydroxyl radicals. II | Proceedings of the Royal Society of London A: Mathematical, Physical and...
Studies of the reactions of hydroxyl radicals. II Message Subject (Your Name) has sent you a message from Proceedings of the ... Studies of the reactions of hydroxyl radicals. II. D. E. Hoare, G. B. Peacock ... methyl radicals combine to give ethane which in turn reacts with hydroxyl radicals twelve times more rapidly than does methane. ... The homogeneous decomposition of hydrogen peroxide has been used as a source of hydroxyl radicals. In part I values were ...
Comparative QSAR study on hydroxyl radical reactivity with unsaturated hydrocarbons: PLS versus MLR
NIOSHTIC-2 Publications Search - 20025082 - Efficacy of taurine and hypotaurine as hydroxyl radical scavengers.
1990). In another study. AM loaded with taurine prior to oxidant stress were found to be less susceptible to oxidant injury (J ... 1991). In this study. we investigated the radical scavenger activity of taurine, and its metabolic precursor hypotaurine (2- ... Electron spin resonance (ESR) was used to investigate the reoction of taurine and hypotaurine with hydroxyl radicals (OH). The ... Free-radicals; Free-radical-generation; Cell-function; Cell-damage; Cell-biology; Cellular-reactions; Lipid-peroxidation; ...
NIOSHTIC-2 Publications Search - 20023076 - Effect of chlorogenic acid on hydroxyl radical.
However, the inhibitory effects of CGA on specific radical species are not well understood. Electron spin resonance (ESR) in ... combination with spin trapping techniques was utilized to detect free radicals. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was ... The rate constant for the scavenging of *OH by CGA was 7.73 x 10(9) M(-1) sec(-1). Our studies suggest that the antioxidant ... was used as a spin trapping reagent while the Fenton reaction was used as a source of hydroxyl radical (*OH). We found that CGA ...
Alaternin and emodin with hydroxyl radical inhibitory and/or scavenging activities and hepatoprotective activity on tacrine...
... to scavenge and/or inhibit hydroxyl radicals generated by the Fenton reaction and to protect tacrine-induced cytotoxicity in ... Comparative Study * Research Support, Non-U.S. Govt MeSH terms * Cell Line, Tumor ... The hydroxyl radical scavenging activity was determined by electron spin resonance spectroscopy using 5,5-dimethy-1-pyrroline-N ... Although the scavenging activity of alaternin on hydroxyl radical was similar to that of emodin in dose-dependent patterns, the ...
Chu, S.-I.; Yoshimine, M.; Liu, B., Ab initio study of the X2«PI» and A2«SIGMA»+ states of OH. I. Potential curves and ... Hinkley, R.K.; Walker, T.E.H.; Richards, W.G., On the e.p.r. spectrum of vibrationally excited hydroxyl radicals, Proc. R. Soc ... Clough, P.N.; Curran, A.H.; Thrush, B.A., The e.p.r. spectrum of vibrationally excited hydroxyl radicals, Proc. R. Soc. London ... Destombes, J.-L.; Marliere, C.; Rohart, F.; Burie, J., Nouvelle analyse du spectre hertzien du radical hydroxyl, C.R. Acad. Sci ...
What are the American Association for the Study of Liver Diseases (AASLD) diagnostic guidelines for fatty liver disease?
The 2010 American Association for the Study of Liver Diseases (AASLD) practice guideline for alcoholic liver disease (ALD) ... IL = interleukin ; NO = nitric oxide; O2- = superoxide anion; OH- = hydroxyl radical; PMN = polymorphonuclear lymphocyte; TNF ... Open-label pilot study of folic acid in patients with nonalcoholic steatohepatitis. Liver Int. 2007 Mar. 27(2):220-6. [Medline] ... BS Anand, MD is a member of the following medical societies: American Association for the Study of Liver Diseases, American ...
Zeitschrift für Naturforschung C
The Kinetics of Hydroxyl-Radical-Induced Strand Breakage of Hyaluronic Acid. A Pulse Radiolysis Study Using Conductometry and ... Further Studies on the Biosynthesis of Pseudoisoeugenols in Tissue Cultures of Pimpinella anisum. Reichling, Jürgen / Martin, ... Fluorescence Studies on Lung Tumors. Lohmann, Wolfgang / Hirzinger, Burkhard / Schwemmle, Konrad / Muhrer, Karl-Heinz / Schulz ... The Structure of Lipids and Proteins Studied by Attenuated Total Reflection (ATR) Infrared Spectroscopy II. Oriented Layers of ...
Molecules | Free Full-Text | The Synthesis and Evaluation of Novel Hydroxyl Substituted Chalcone Analogs with in Vitro Anti...
... we synthesized a series of novel hydroxyl chalcone analogs which possessed anti-free radical activity, and screened their ... The indication of C7s effect on AD needs further study. ... and OH free radicals in vitro. Compound C7, 4,2-dihydroxy-3,5- ... dimethoxychalcone was found to have potent activity in these anti-free radical activity tests. Further research revealed that ... pathogenesis involves an imbalance between free radical formation and destruction. In order to obtain a novel preclinical anti- ...
Search Results - - 33 Results - Digital Library
Kinetic and modeling studies of the reaction of hydroxyl radicals with tetrachloroethylene Description: Article on kinetic and ... Reaction of hydroxyl radicals with trichloroethylene: Evidence for chlorine elimination channels at elevated temperatures ... Description: Article on the reaction of hydroxyl radicals with trichloroethylene and evidence for chlorine elimination channels ... Description: In this article, the authors study the entropy time evolution of a quantum mechanical model, which is frequently ...
Holzforschung - International Journal of the Biology, Chemistry, Physics and Technology of Wood
Hydroxyl Radical Activity in Brown-Rot Fungi Studied by a New Chemiluminescence Method. Backa, Stefan / Gierer, Josef / ... A Study of Natural and In Vitro Degradation. Barrasa, José M. / González, Aldo E. / Martínez, Angel T. ... Studies on the Mechanism of the Photoyellowing of Bleached Mechanical and Chemimechanical Pulps. IV. The Role of Ring- ... 13C NMR Spectroscopic Studies of Phenylcoumaran and 1,2-Diarylpropane Type Lignin Model Compounds. Part 2. Substituent Effects ...
Formation of superoxide and hydroxyl radicals from 1-methyl-4-phenylpyridinium ion (MPP+): reductive activation by NADPH...
... has been studied using spin-trapping techniques. Incubation of MPP+ with purified NADPH cytochrome P-450 reductase and NADPH ... a significant stimulation of superoxide and hydroxyl radicals was detected. Formation of these toxic radicals from MPP+ was ... Under identical conditions, however, considerably less of these radicals were formed with MPP+ in comparison to paraquat, a ... under anaerobic conditions failed to produce any detectable radical intermediates. However, in the presence of air and a spin- ...
A study of retinal oxidation : the effects of melatonin and the roles of nitric oxide and hydroxyl radicals | PolyU...
A study of retinal oxidation : the effects of melatonin and the roles of nitric oxide and hydroxyl radicals. ... The damaging effects of NO* on the retinal tissues were compared with those of hydroxyl radicals (*OH). The protective role of ... Further studies are indicated to investigate the role of oxidation in retinal damage and the applications of MaT (and other ... A role for free radical oxidation in the pathogenesis of glaucoma is further supported by the dose-dependent increase in ...
Ab initio study of atmospheric reactions of the hydroxyl radical-water complex (OH-H2O) with saturated hydrocarbons (methane,...
Ab initio study of atmospheric reactions of the hydroxyl radical-water complex (OH-H2O) with saturated hydrocarbons (methane, ... Ab initio study of atmospheric reactions of the hydroxyl radical-water complex (OH-H2O) with saturated hydrocarbons (methane, ... Weakly bound reactant and product complexes in the hydrogen abstraction reactions of the hydroxyl radical-water complex (OH-H2O ... of the complexes and the transition states were optimized at the UB3LYP level for the reactions of a hydroxyl radical-water ...
Spectral, kinetic, and redox studies on the transients formed on reaction of hydroxyl radicals with thioanisole - Publications...
Spectral, kinetic, and redox studies on the transients formed on reaction of hydroxyl radicals with thioanisole ... and redox studies on the transients formed on reaction of hydroxyl radicals with thioanisole Journal of Physical Chemistry A, ... The hydroxyl radicals are observed to react with thioanisole (TA) with a bimolecular rate constant of 3.5 × 109 dm3 mol-1 s-1 ... Based on the studies with specific one-electron oxidants, ≈73% of •OH radicals are inferred to react with thioanisole by ...
Frontiers | Alternative kynurenic acid synthesis routes studied in the rat cerebellum | Frontiers in Cellular Neuroscience
In chemical combinatorial systems, both L-KYN and D-KYN interacted directly with peroxynitrite (ONOO-) and hydroxyl radicals ( ... In chemical combinatorial systems, both L-KYN and D-KYN interacted directly with peroxynitrite (ONOO-) and hydroxyl radicals ( ... In chemical combinatorial systems, both L-KYN and D-KYN interacted directly with peroxynitrite (ONOO−) and hydroxyl radicals ( ... 2006). Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide ...
Photochemical oxidatio n of di- n-butyl phthalate i n atmospheric hydrometeors by hydroxyl radicals from nitrous acid |...
OH radicals from nitrous acid (HONO) in atmospheric hydrometeors was explored by two techniques, steady-state irradiation, and ... Reaction of hydroxyl radical with aromatic hydrocarbons in nonaqueous solutions: a laser flash photolysis study in acetonitrile ... On the electrophilicity of hydroxyl radical: a laser flash photolysis and computational study. J Am Chem Soc 127:7094-7109. ... Di-butyl phthalate Nitrous acid Hydroxyl radicals Kinetics Mechanisms Atmospheric hydrometeors Responsible editor: Gerhard ...
Antioxidants | Free Full-Text | Cerium Oxide Nanoparticles: A Brief Review of Their Synthesis Methods and Biomedical...
... that offer enhanced biocompatibility and a need for establishing a reference CeNP material for consistency across studies; (2) ... Although the bio-mimetic activities of CeNPs have been extensively studied in vitro, in vivo interactions and associated ... 3.2.1. Scavenging Hydroxyl Radicals. The hydroxyl radical is one of the most biologically active free radicals . CeNPs have ... 3.2.2. Scavenging Nitric Oxide Radicals and Peroxynitrite. The nitric oxide radical is a gaseous free radical with both ...
兰州大学机构知识库: Reaction of hydroxyl radical with phenylpropanoid glycosides from Pedicularis species: A pulse radiolysis study
Reaction of hydroxyl radical with phenylpropanoid glycosides from Pedicularis species: A pulse radiolysis study[J]. SCIENCE IN ... Reaction of hydroxyl radical with phenylpropanoid glycosides from Pedicularis species: A pulse radiolysis study. ... suggesting that they are effective hydroxyl radical scavengers. The results demonstrate that the numbers of phenolic hydroxyl ... phenylpropanoid glycosides ; Pedicularis ; hydroxyl radical ; pulse radiolysis ; rate constant ; scavenging effect 作者部门: ...
Search Results - - 10,472 Results - Digital Library
Kinetic and Mechanistic Studies of the Reaction of Hydroxyl Radicals with Acetaldehyde over an Extended Temperature Range ... Description: This article discusses kinetic and mechanistic studies of the reaction of hydroxyl radicals with acetaldehyde over ... A computational study of the reaction kinetics of methyl radicals with trifluorohalomethanes Description: This article ... An ab Initio Study of Sulfinic Acid and Related Species Description: Article on an ab initio study of sulfinic acid and related ...
Rapid Killing of Acinetobacter baumannii by Polymyxins Is Mediated by a Hydroxyl Radical Death Pathway | Antimicrobial Agents...
... this study may provide clues as to how to exploit hydroxyl radical-mediated cell death to combat drug resistance in this and ... through hydroxyl radical production. Treatment of A. baumannii with these antibiotics caused an increase in hydroxyl radicals ... their ability to both induce hydroxyl radicals and kill A. baumannii through hydroxyl radical production is somewhat surprising ... S5A to C in the supplemental material). Since quenching hydroxyl radicals delays killing, these data indicate that the radicals ...
Hydroxyl Radical Generation and Contaminant Removal from Water by the Collapse of Microbubbles Under Different Hydrochemical...
The present study addresses the mechanism of hydroxyl radical (·OH) generation by the collapse of microbubbles in water ... The present study addresses the mechanism of hydroxyl radical (·OH) generation by the collapse of microbubbles in water ... Microbubble Methylene blue Degradation Hydroxyl radical pH Ionic strength This is a preview of subscription content, log in to ... Hydroxyl Radical Generation and Contaminant Removal from Water by the Collapse of Microbubbles Under Different Hydrochemical ...
Hydroxyl radical - Wikipedia
OH radical is linked with the production of H2O in molecular clouds. Studies of •OH distribution in Taurus Molecular Cloud-1 ( ... The hydroxyl radical, •OH, is the neutral form of the hydroxide ion (OH−). Hydroxyl radicals are highly reactive (easily ... the hydroxyl radical cannot be eliminated by an enzymatic reaction. The hydroxyl •OH radical is one of the main chemical ... hydroxyl radicals are most commonly generated by photolysis of 1-hydroxy-2(1H)-pyridinethione. The hydroxyl radical is often ...
IJMS | Free Full-Text | Antioxidant Properties and PC12 Cell Protective Effects of a Novel Curcumin Analogue (2E,6E)-2,6-Bis(3...
... radical scavenging and PC12 cell protection from H2O2 damage. MCH displayed superior O2•− quenching abilities compared to ... In the present study, we evaluated the antioxidant potential of MCH by DPPH/superoxide/hydroxyl radical scavenging, observed ... Hydroxyl Radical Scavenging Assay. The hydroxyl radical scavenging activity was measured using the Fenton reaction assay as ... H2O2 can generate detrimental hydroxyl radicals and increase the ROS levels in cells. In this study, treatment of PC12 cells ...
A self-illuminating nanoparticle for inflammation imaging and cancer therapy | Science Advances
Hydroxyl radical (•OH) was generated by the Fenton reaction between ferrous acetate and H2O2, while superoxide anion was ... In vivo antitumor studies of the CLP nanoparticle. We then examined the in vivo antitumor efficacy of CLP nanoparticles in mice ... In another study, excretion of CLP was examined in BALB/c mice that were housed individually in metabolic cages. Briefly, after ... In a separate study, we compared in vivo efficacy of CLP nanoparticles with a typical chemotherapy drug cisplatin (CDDP) in ...
NIOSHTIC-2 Search Results - Full View
Free-radical-generation; Hydroxyl-groups; In-vitro-studies ... The hydroxyl radical spin trap molecule was 5,5-dimethyl-1- ... The inclusion of free radical scavengers and metal ion chelators affected the amount of hydroxyl radicals generated; while EDTA ... The authors conclude that these findings point to a dominant role of the Fenton reaction in the generation of hydroxyl radicals ... Hydroxyl radical generation by coal mine dust: possible implication to coal workers pneumoconiosis (CWP). ...
Lipid peroxidationKineticsReaction of hydroxyl radicalsHydrogenMechanismReactivityReactive oxygenOxidationEnhanced hydroxyl radical generationScavengeVitroAtmosphericAmount of hydroxylLevels of hydroxylNitricAnionMolecularReactionsFentonDecompositionOxygenDegradationConcentrationsFreeSituPhotolysisPeroxynitriteOxidizeOxidantsAntioxidantExperimentallyScavengersOrganicReactInvestigateSpeciesNucleic acidsProteinReactsHydroxideGenerationCleavageToxicityMethaneChemistryElectronComplexesNitrousFormationMoleculeInhibitoryAtomsSpin-trapProteinsHONO
- The results show that taurine does not scavenge OH radicals nor prevent lipid peroxidation at concentrations up to 20 roM. (cdc.gov)
- In contrast, hypotaurine scavenged OH radicals with reaction rate constant of k = 1.6 X 10(10)M-1S-1 and protected against lipid peroxidation in a conccn- tration-dependcnt manner from 0.5-20 mM. (cdc.gov)
- Retinal susceptibility to free radical damage was also compared with those of two other tissues - kidney and liver, the levels of malondialdehyde (MDA) produced per unit weight protein providing an index of lipid peroxidation (LPO). (edu.hk)
- Surface iron (7439896) content of coal mine dusts was positively correlated with hydroxyl radical generating potential and potential to induce lipid peroxidation. (cdc.gov)
- The hydroxyl radical can damage virtually all types of macromolecules: carbohydrates, nucleic acids (mutations), lipids (lipid peroxidation), and amino acids (e.g. conversion of Phe to m-Tyrosine and o-Tyrosine). (wikipedia.org)
- On the other hand, excessive exercise leads to the production of endogenous oxidative free radicals and lipid peroxidation, which can damage the cell membrane system [ 8 - 10 ]. (hindawi.com)
- In studies of eukaryotic cells treated with excess copper, workers detected elevated levels of DNA lesions, protein oxidation, lipid peroxidation, and reactive oxygen species generation ( 3 , 36 , 53 , 55 , 59 ). (asm.org)
- A study found that patients with chronic obstructive pulmonary disease (COPD) had a decrease in oxidative stress, including glutathione oxidation and lipid peroxidation, when xanthine oxidase was inhibited using allopurinol. (wikipedia.org)
- β-amyloid associated free radicals can initiate lipid peroxidation, protein oxidation, ROS formation, intracellular and mitochondrial Ca 2+ accumulation and eventually lead to death of neurons [ 20 - 24 ]. (omicsonline.org)
- Together with the observation that alcoholic patients have increased indices of lipid peroxidation, there is evidence suggestive of free radical (i.e., unpaired electrons or reactive oxygen species) mediated damage in the pathogenesis of alcohol-induced muscle disease. (bioscience.org)
- The Kinetics of Hydroxyl-Radical-Induced Strand Breakage of Hyaluronic Acid. (degruyter.com)
- In addition, the kinetics of the reverse processes for the latter two elementary reactions are also studied experimentally. (unt.edu)
- Heterogeneous Kinetics of cis-Pinonic Acid with Hydroxyl Radical under Different Environmental Conditions. (semanticscholar.org)
- Supplementary data for the mechanism research for depolymerization of cellulose induced by hydroxyl radical using GC-MS, reaction kinetics simulation and quantum chemistry computation. (nih.gov)
- Their technique should enable more structural information to be obtained from studies of protein-folding kinetics - crucial for developing the next generation of computational methods for simulating protein dynamics, and to allow more complex proteins and protein complexes to be studied experimentally. (nature.com)
Reaction of hydroxyl radicals2
- In part I values were reported for the relative rates of reaction of hydroxyl radicals with methane, carbon monoxide, formaldehyde and hydrogen peroxide. (royalsocietypublishing.org)
- Mittal, Jai P. (1997) Spectral, kinetic, and redox studies on the transients formed on reaction of hydroxyl radicals with thioanisole Journal of Physical Chemistry A, 101 (51). (ias.ac.in)
- The homogeneous decomposition of hydrogen peroxide has been used as a source of hydroxyl radicals. (royalsocietypublishing.org)
- Weakly bound reactant and product complexes in the hydrogen abstraction reactions of the hydroxyl radical-water complex (OH-H2O) with CH4, C2H6, and C3H8 were investigated with ab initio molecular orbital methods. (cumhuriyet.edu.tr)
- A compilation of specific biomolecular rate constants for the reactions of hydrated electrons, hydrogen atoms and hydroxyl radicals with inorganic and organic compounds in aqueous solutions. (cdc.gov)
- The temperature dependences of these reactions are interpreted via the Arrhenius equation, which yields significantly negative activation energies for the reaction of the chlorine atom and hydrogen sulfide as well as for that between the phenyl radical and hydrogen chloride. (unt.edu)
- Electron spin resonance was used in conjunction with spin trapping techniques to measure the generation of hydroxyl radicals from hydrogen-peroxide in the presence of the dust samples. (cdc.gov)
- The coal mine dust samples all generated varying levels of hydroxyl radicals from hydrogen-peroxide. (cdc.gov)
- It is also an important radical formed in radiation chemistry, since it leads to the formation of hydrogen peroxide and oxygen, which can enhance corrosion and SCC in coolant systems subjected to radioactive environments. (wikipedia.org)
- Hydrogen effects have been solely ascribed to exclusive removal of hydroxyl radical. (unboundmedicine.com)
- In the immediate-type allergic reaction, hydrogen exerts its beneficial effect not by its radical scavenging activity but by modulating a specific signaling pathway. (unboundmedicine.com)
- Our studies also suggest that hydrogen is a gaseous signaling molecule like nitric oxide. (unboundmedicine.com)
- A further requirement is to generate hydroxyl radicals from the bulk solvent (i.e. water) (equations 1 and 2) not hydrogen peroxide which can remain to oxidize proteins even without other stimuli. (wikipedia.org)
- Photodegradation will account for removal of 1,2-dichloropropane from the atmosphere, based on a calculated half-life of 34.9 days at 25 C. The reaction with OH radicals proceeds via net hydrogen abstraction from C-H bonds. (europa.eu)
- Hydroxyl and alkyl-oxy radicals were generated to a high degree of purity by illuminating a phosphate buffer solution containing hydrogen peroxide and 2,2'-azobis(2-amidinopropane) dihydrochloride with a Hg-Xe arc lamp. (news-medical.net)
- Comparative molecular dynamics study of fcc-Al hydrogen embrittlement Corrosion Science Published online. (psu.edu)
- Two reaction types have been studied as follows: addition to the aromatic ring atoms and hydrogen/proton abstraction. (diva-portal.org)
- In discussing the history of oxidants, such as hydrogen peroxide, respected free radical research pioneer, Barry Halliwell, said that, "In the beginning, it was simple: we said free radicals are bad, antioxidants are good. (ispub.com)
- Hydrogen has been proven to be a novel antioxidant through its selectively reducing of the hydroxyl radical. (nih.gov)
- In this study, we investigated the effects of hydrogen-rich saline on the prevention of acute lung injury induced by hyperoxia (HALI) in rats. (nih.gov)
- The results of this study demonstrate that hydrogen-rich saline ameliorated hyperoxia-induced acute lung injury by reducing oxidative stress and inflammatory cascades in lung tissue. (nih.gov)
- While the hydroxyl radical, generated by accelerated decomposition of ozone by addition of hydrogen peroxide or by raising the pH, was found more effective in the case of alachlor. (asce.org)
- The generation of radicals on the BNCT conditions could play an important role in therapeutical mechanism. (springer.com)
- Studies on the Mechanism of the Photoyellowing of Bleached Mechanical and Chemimechanical Pulps. (degruyter.com)
- Here, we present an alternative mechanism based on data demonstrating that polymyxins induce rapid cell death through hydroxyl radical production. (asm.org)
- The present study addresses the mechanism of hydroxyl radical (·OH) generation by the collapse of microbubbles in water solution. (springer.com)
- Cl − in the chlorine radical-initiated DNA guanine (G) oxidation reaction, and provide direct evidence to ascertain the intricate type of addition/charge separation mechanism underlying guanine oxidation. (sciencemag.org)
- In this analogy, hydroxyl represents part of the draining mechanism in the sink. (eurekalert.org)
- Finally, estrogens could also serve as free-radical scavengers (i.e., by an ER-independent mechanism) in preventing nerve-cell death induced by various oxidative insults ( 11 - 14 ). (pnas.org)
- However, this mechanism, including the chemical nature and fate of the products derived from the radical-scavenging reaction(s), has not been elucidated. (pnas.org)
- We recently studied a 7-yr-old patient with recurrent sinopulmonary infections in whom we documented the mechanism by which the C4A*Q0,B*Q0 double null haplotype arose. (jci.org)
- The mechanism of degradation for many organic contaminants by sulfate radicals is well studied, but the final mineralization products are not commonly evaluated. (uni-due.de)
- Tribochemical Mechanism of Amorphous Silica Asperities in Aqueous Environment: A ReaxFF Molecular Dynamics Study. (psu.edu)
- In the present work, we found that ferripyochelin (FePCH), the chelate complex of PCH with Fe 3+ , could significantly increase TPT decomposition in comparison with PCH, and further studies were conducted to elucidate the possible mechanism. (asm.org)
- Harman published his free radical theory of aging in 1956 and in the following decades it slowly became probably the most popular explanation of the mechanism of aging (Ashok & Ali, 1999). (longecity.org)
- Increasing evidence suggests that oxidative stress may play an important role in the toxicity mechanism of MCs on fish, but most studies were based on relatively high. (ebscohost.com)
- Little is known, though, about the detailed chemical mechanism of RNA cleavage by the hydroxyl radical. (jbsdonline.com)
- These experiments provide the first direct evidence for the mechanism of RNA cleavage by the hydroxyl radical. (jbsdonline.com)
- In the rat, circulating troponin-T release increases in the presence of ethanol, a mechanism ascribed to free radical mediated damage, as it is prevented with the xanthine oxidase inhibitor and beta-blocker, propranolol. (bioscience.org)
- The hydroxyl radical has a very short in vivo half-life of approximately 10−9 seconds and a high reactivity. (wikipedia.org)
- Peroxynitrite decomposition generates a strong oxidant with reactivity similar to hydroxyl radical, as assessed by the oxidation of deoxyribose or dimethyl sulfoxide. (pnas.org)
- In this study, we have analyzed the reactivity of pyridoxine toward the ROS .OH, .OOH, and .O2- at the density functional theory level (functionals B3LYP and MPW1B95). (diva-portal.org)
- 13- Demiryürek AT, Cinel İ, Kahraman S, Tecder-Ünal M, Göğüş N, Aypar Ü, Kanzık İ. (1998) Propofol and intralipid interact with reactive oxygen species: A chemiluminescence study. (gantep.edu.tr)
- Oxidative damage from reactive oxygen species (ROS) and the carbon-centred radicals arising from them is important to the process of aging, and age-related diseases are generally caused, exacerbated or mediated by oxidative stress. (ebscohost.com)
- In this study, we shed light on the initial addition of hydroxyl radicals (HO˙) to multiple carboxylated and hydroxylated benzene compounds in aqueous-phase advanced oxidation processes (AOPs). (rsc.org)
- Pan Y, Chen Y, Li Q, Yu X, Wang J, Zheng J. The Synthesis and Evaluation of Novel Hydroxyl Substituted Chalcone Analogs with in Vitro Anti-Free Radicals Pharmacological Activity and in Vivo Anti-Oxidation Activity in a Free Radical-Injury Alzheimer's Model. (mdpi.com)
- Nitric oxide (NO*) was studied as a possible causative factor of retinal oxidation. (edu.hk)
- Hydroxyl radical induced oxidation of nitrobenzene. (cdc.gov)
- A critical feature of these experiments is the need to expose proteins to hydroxyl radicals for limited timescales on the order of 1-50 ms inducing 10-30% oxidation of total protein. (wikipedia.org)
- In the atmosphere, the oxidation of the chloropropanes studied would be expected to lead to the formation of chlorinated carbonyls such as HC(O)Cl, CH3C(O)Cl, CH and3C(O)CH2Cl, CH2ClC(O)CH2Cl CH2ClCHClC(O)Cl. (europa.eu)
- XO has also been found to produce the strong one-electron oxidant carbonate radical anion from oxidation with acetaldehyde in the presence of catalase and bicarbonate. (wikipedia.org)
- Labelling molecules by fast oxidation allows mass spectrometry to study protein folding at submillisecond time resolution. (nature.com)
Enhanced hydroxyl radical generation2
- while EDTA and diethylenetriaminepentaacetic-acid enhanced hydroxyl radical generation, catalase and deferoxamine produced almost total inhibition and superoxide-dismutase produced partial inhibition. (cdc.gov)
- A kinetic study of 3-chlorophenol enhanced hydroxyl radical generation during ozonation. (nih.gov)
- The antioxidative and hepatoprotective potentials of two anthraquinones, alaternin (2-hydroxyemodin) and emodin, to scavenge and/or inhibit hydroxyl radicals generated by the Fenton reaction and to protect tacrine-induced cytotoxicity in human liver derived HepG2 cells were evaluated, respectively. (nih.gov)
- In addition, the ability of betalains in this vegetable to scavenge free radicals-including hydroxyl radicals-has been recently reaffirmed. (whfoods.org)
- In order to obtain a novel preclinical anti-AD drug candidate, we synthesized a series of novel hydroxyl chalcone analogs which possessed anti-free radical activity, and screened their effects on scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH free radicals in vitro . (mdpi.com)
- Although the bio-mimetic activities of CeNPs have been extensively studied in vitro, in vivo interactions and associated protein corona formation are not well understood. (mdpi.com)
- The antioxidative properties of a novel curcumin analogue (2 E ,6 E )-2,6-bis(3,5-dimethoxybenzylidene)cyclohexanone (MCH) were assessed by several in vitro models, including superoxide anion, hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and PC12 cell protection from H 2 O 2 damage. (mdpi.com)
- Because copper catalyzes the conversion of H 2 O 2 to hydroxyl radicals in vitro, it has been proposed that oxidative DNA damage may be an important component of copper toxicity. (asm.org)
- Hydroxyl radical (.OH) formation by neutrophils in vitro requires exogenous iron. (biochemj.org)
- The samples such as ethyl acetate and ethanol extracts were tested using six in vitromodels such as 2,2-diphenyl-1-picrylhydrazyl, nitric oxide radical, iron chelating, hydroxyl radical, superoxide radical scavenging activity and total antioxidant activity to evaluate the in vitro antioxidant potential of C. retusa by spectrophotometrically. (ijpsonline.com)
- The aim of the study was to investigate the effect of different anethole concentrations on the in vitro culture of caprine preantral follicles enclosed in ovarian tissue. (scielo.br)
- In order to recover and use these follicles, preantral follicle culture is an important tool to study in vitro folliculogenesis, including the effect of different substances ( 2 ). (scielo.br)
- Studies demonstrated that follicles cultured in vitro have lower developmental capability than those grown in vivo ( 3 ). (scielo.br)
- Several studies have shown that high ROS levels produced during in vitro culture impair follicle development by damaging their cellular and molecular structures (for review, see Agarwal et al. (scielo.br)
- This included 19 in vitro studies, 15 animal studies and two human studies. (deepdyve.com)
- OH radicals from nitrous acid (HONO) in atmospheric hydrometeors was explored by two techniques, steady-state irradiation, and laser flash photolysis (LFP). (springer.com)
- Most notably hydroxyl radicals are produced from the decomposition of hydroperoxides (ROOH) or, in atmospheric chemistry, by the reaction of excited atomic oxygen with water. (wikipedia.org)
- During the early 2000s, environmental scientists studying methane emissions noticed something unexpected: the global concentrations of atmospheric methane (CH4)--which had increased for decades, driven by methane emissions from fossil fuels and agriculture--inexplicably leveled off. (eurekalert.org)
- When atmospheric concentrations of methane increase, it may not be correct to chalk it up solely to an increase in methane emissions, says Caltech's Christian Frankenberg, co-corresponding author of a study on the decadal trends of methane concentrations that was published the week of April 17 in the early online edition of the Proceedings of the National Academy of Sciences . (eurekalert.org)
- The PNAS study is titled "Ambiguity in the causes for decadal trends in atmospheric methane and hydroxyl. (eurekalert.org)
- Hydroxyl radicals can be produced in solution by an electrical discharge within a conventional atmospheric pressure electrospray ionization (ESI) source. (wikipedia.org)
- The calculated atmospheric half-life of 1,2-dichloropropane for the reaction with OH radicals is 34.9 days, based on an OH radical concentration of 5 x 10e5 molecules/cm3 and 24 hour photoday. (europa.eu)
Amount of hydroxyl1
Levels of hydroxyl1
- Both tea flower and tea leaf extracts exert their strong hydroxyl radical scavenging effects in the Fenton reaction system and nitric oxide suppressing effects in LPS-induced RAW 264.7 cells. (unboundmedicine.com)
- Through total uptake calculations and mass dependent studies, nitric-acid diffusion into the underlying layers is shown to be an important process for all of the systems studied. (semanticscholar.org)
- Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. (pnas.org)
- Half maximal inhibitory concentration (IC 50) of the ethanol extract was found to be 596 μg/ml for DPPH, 597 μg/ml for nitric oxide radical, 554 μg/ml for iron chelating, 580 μg/ml for hydroxyl radical, 562 μg/ml for superoxide radical and 566 μg/ml for total antioxidant capacity. (ijpsonline.com)
- Recently, we have reported the generation of superoxide anion-radicals by neutron irradiation and UV light irradiation of mercaptoborates in water solutions at room temperature 1 . (springer.com)
- The generation of hydroxyl radicals ·OH, superoxide anion-radicals O 2 · − , and singlet molecular oxygen 1 O 2 were identified and determined by electron spin resonance spectroscopy. (springer.com)
- It is well known that excessive accumulation of free radicals, such as the alkyl-oxy radical, hydroxyl radical and superoxide anion cause significant damage to the human body resulting in numerous diseases and premature aging 2 . (news-medical.net)
- Molecular structures of the complexes and the transition states were optimized at the UB3LYP level for the reactions of a hydroxyl radical-water complex with a series of hydrocarbons. (cumhuriyet.edu.tr)
- In situ photoformation of hydroxyl radical and singlet molecular oxygen. (springer.com)
- Inactivation of the endotoxic biomolecule lipid A by oxygen plasma species: a reactive molecular dynamics study Plasma Processes and Polymers 12, 162-171. (psu.edu)
- Comparative molecular dynamics study of fcc-Ni nanoplate stress corrosion in water Surface Science 633, 94-101. (psu.edu)
- Thermal Decomposition of a Large Fullerene: A Molecular Dynamics Study Using the ReaxFF Reactive Force Field. (psu.edu)
- Interactions of plasma species on nickel catalysts: a reactive molecular dynamics study on the influence of temperature and surface structure. (psu.edu)
- namely, heptachior and alachlor were effectively oxidized by either molecular ozone or the hydroxyl free radical. (asce.org)
- Studies of the reactions of hydroxyl radicals. (royalsocietypublishing.org)
- Kinetic studies and computational modeling of atomic chlorine reactions in the gas phase. (unt.edu)
- Although the radical ion pair has been frequently invoked as a key intermediate in DNA oxidative damage reactions and photoinduced electron transfer processes, the unambiguous detection and characterization of this species remain formidable and unresolved due to its extremely unstable nature and low concentration. (sciencemag.org)
- 1,2-Dichloropropane undergoes reactions with hydroxyl radicals in the atmosphere. (europa.eu)
- The Fenton-reaction (Fe(II) + H2O2 Fe(III) + OH + -OH) and the Cr(V)-mediated Fenton-like reaction (Cr(V) + H2O2-Cr(VI) + OH + -OH) were used as sources of OH radicals. (cdc.gov)
- 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was used as a spin trapping reagent while the Fenton reaction was used as a source of hydroxyl radical (*OH). (cdc.gov)
- Hydroxyl radicals are also produced during UV-light dissociation of H2O2 (suggested in 1879) and likely in Fenton chemistry, where trace amounts of reduced transition metals catalyze peroxide-mediated oxidations of organic compounds. (wikipedia.org)
- Electron paramagnetic resonance (EPR) spin trapping showed that the copper-dependent H 2 O 2 resistance was not caused by inhibition of the Fenton reaction, for copper-supplemented cells exhibited substantial hydroxyl radical formation. (asm.org)
- The quantity of generated radicals depend on initial oxygen concentration in irradiated solutions and on the energy of used neutron beam. (springer.com)
- The reaction of methyl radicals with oxygen has previously been shown to occur by a third order reaction both at 200 °C (Hoare & Walsh 1957) and at room temperature (Christie 1958). (royalsocietypublishing.org)
- In the absence of added oxygen, methyl radicals combine to give ethane which in turn reacts with hydroxyl radicals twelve times more rapidly than does methane. (royalsocietypublishing.org)
- The alkyl radical will typically react rapidly with oxygen forming a peroxy radical. (wikipedia.org)
- It is described as a "radical" because it is highly reactive and, as such, acts like a detergent in the atmosphere, breaking down methane into oxygen and water vapor. (eurekalert.org)
- Other studies have extended the method to study early onset protein damage given the radical basis of the method and the significance of oxygen based radicals in the pathogenesis of many diseases including neurological disorders and even blindness. (wikipedia.org)
- Increasing number of experimental and clinical studies present convincing evidence that hyperbaric oxygen therapy (HBOT), as an adjunctive therapy, may be the suitable neurotherapeutic method for improving neurological outcome after TBI. (intechopen.com)
- Mao, Yuxiang 2017-04-03 00:00:00 Singlet oxygen (1O2) and hydroxyl radical (·OH) play an important role in the degradation of pollutants in surface waters. (deepdyve.com)
- The organic hydrocarbon contamination is rapidly oxidized by the hydroxyl radical to carbon dioxide, oxygen, and water. (environmental-expert.com)
- Studies of irradiated bacterial spores in aqueous suspension indicate that the sensitization of spores by oxygen can depend on three chemical processes. (sciencemag.org)
- OH mediated degradation of dimethyl phthalate in aqueous solution: experimental and theoretical studies. (springer.com)
- Arranged in ten chapters, the book presents detailed studies that can help readers understand the attack and degradation mechanisms of polymer membranes and mitigation strategies. (wiley.com)
- Global average hydroxyl radical concentrations have been measured indirectly by analyzing methyl chloroform (CH3CCl3) present in the air. (wikipedia.org)
- Tracking decadal trends in both methane and hydroxyl, Frankenberg and his colleagues noted that fluctuations in hydroxyl concentrations correlated strongly with fluctuations in methane. (eurekalert.org)
- However, the authors do not yet have a mechanistic explanation for the last decade's global changes in hydroxyl concentrations. (eurekalert.org)
- The pilot study results indicate that this innovative in situ remediation technology can effectively lower chlorinated solvent concentrations in source areas located within a limestone bedrock aquifer. (environmental-expert.com)
- In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. (ijpsonline.com)
- Paradoxically, alcohol-feeding studies in rats have shown that the imidazole dipeptide concentrations are increased in myopathic muscles though alpha-tocopherol contents are not significantly altered. (bioscience.org)
- Electron spin resonance (ESR) in combination with spin trapping techniques was utilized to detect free radicals. (cdc.gov)
- The inhibitory activity on hydroxyl radical generated in a cell-free chemical system (FeSO4/H2O2) was investigated by a fluorescence spectrophotometer using a highly fluorescent probe, 2',7'-dichlorofluorescein. (nih.gov)
- Alzheimer's disease (AD) pathogenesis involves an imbalance between free radical formation and destruction. (mdpi.com)
- Compound C7 , 4,2'-dihydroxy-3,5-dimethoxychalcone was found to have potent activity in these anti-free radical activity tests. (mdpi.com)
- The work reported here was to investigate free radical-induced damage in the retinal tissues and related protective phenomenon. (edu.hk)
- Elevated NO* production is potentially threatening to the retina, which is more susceptible to free radical-induced LPO than kidney and liver. (edu.hk)
- Formation of free radical intermediates from 1--methyl-4-phenylpyridinium ion(MPP+) has been studied using spin-trapping techniques. (semanticscholar.org)
- The influence of gas supply and flow rate, solution pH, and ionic strength on the aeration efficiency, free radical generation, and contaminant removal (take methylene blue as an example) are elucidated. (springer.com)
- Substantial evidence now exists that intrinsic free-radical scavenging contributes to the receptor-independent neuroprotective effects of estrogens. (pnas.org)
- Substantial evidence now exists that estrogen neuroprotection is related to or complemented by the intrinsic free-radical scavenging antioxidant capacity of the hormone due to its A-ring phenolic hydroxy group ( 21 ). (pnas.org)
- As reported, energy metabolism and reactive free radicals accumulation are involved in the pathophysiology of fatigue [ 4 , 5 ]. (hindawi.com)
- The results support the hypothesis that mutant SOD induced-neurodegeneration is associated with disturbances of neuronal free radical homeostasis. (jneurosci.org)
- 1994) Characterization of a method for the detection of drugs with free radical scavenging activity using porcine leukocytes. (gantep.edu.tr)
- 1998) Effect of free radical production and scavengers on occlusion-reperfusion induced arrhythmias. (gantep.edu.tr)
- Toxin effects on DA mitochondria were not dependent upon ATP, calcium, free radical species, JNK, or caspase3/PKC pathways but were completely blocked by the thiol-anti-oxidant N -acetyl-cysteine or membrane-permeable glutathione. (jneurosci.org)
- Free radicals oxidize blood vessel walls, protein molecules, DNA, carbohydrates, and membrane lipids, changing the way cells interact with their environment and reproduce. (news-medical.net)
- Electron spin resonance (ESR) spectroscopy has proved an effective means of directly determining the level of free radicals in food 4 . (news-medical.net)
- However, the free radical scavenging activity of coffee had not been evaluated using this technique. (news-medical.net)
- Myocardial 'reperfusion injury' has been partly attributed to the production of free radicals which are cytotoxic towards cells. (curehunter.com)
- Anyone over 18 years old or over can take the products contained in the Complete Detoxification Kit, especially those who want to support normal liver detoxification activity, help maintain healthy glutathione levels and provide the body with strong antioxidant protection to fight the negative effects of free radicals. (shop.com)
- First, it quenches free radicals created when the liver neutralizes toxins in Phase 1 of detoxification. (shop.com)
- The naïve notion (promulgated by the free radical theory), that EMODs are inherently toxic, is counterintuitive to very basis of the evolutionary concept itself. (ispub.com)
- In fact, recent findings from randomized controlled trials (RCTs) have repeatedly failed to support the free radical theory and some trial results have even shown harmful effects and increased risk of mortality from antioxidant use. (ispub.com)
- From helping maintain healthy cholesterol levels, joint flexibility, cardiovascular health and circulation, nutraMetrix Isotonix OPC-3 helps the body function at its peak by combating free radicals throughout your body. (nutrametrix.com)
- These results clearly indicated that C. retusa is effective against free radical mediated diseases as a natural antioxidant. (ijpsonline.com)
- ROS are free radicals involved in many human diseases. (ijpsonline.com)
- The underlying theory that has been long accepted says that antioxidants may improve health (and eventually prolong lifespan) by lowering the level of oxidative stress present in the organism because they eliminate free radicals, usually by 'donating' a free electron. (longecity.org)
- This method used a nanosecond laser to photochemically generate a burst of hydroxyl radicals in situ in-cell suspension to oxidize the amino acids on the protein surface. (bireme.br)
- A pilot study to evaluate an innovative in situ treatment technology was implemented at Letterkenny Army Depot, Chambersburg, Pennsylvania, for a limestone bedrock aquifer contaminated with chlorinated solvents. (environmental-expert.com)
- In organic synthesis, hydroxyl radicals are most commonly generated by photolysis of 1-hydroxy-2(1H)-pyridinethione. (wikipedia.org)
- The rate of reaction with the hydroxyl radical often determines how long many pollutants last in the atmosphere, if they do not undergo photolysis or are rained out. (wikipedia.org)
- Application of Nanosecond Laser Photolysis Protein Footprinting to Study EGFR Activation by EGF in Cells. (bireme.br)
- Here, we further optimized an ultrafast laser photolysis hydroxyl radical footprinting method and applied it to study the interaction of EGF and EGFR in live mammalian cells. (bireme.br)
- Our footprinting data agreed with the two relevant EGFR crystal structures, indicating that this in-cell laser photolysis footprinting technique is a valid approach to study the structural properties of integral membrane proteins directly in the native environment. (bireme.br)
- These chlorinated carbonyl compounds may be lost by further reaction with OH radicals, by photolysis, or by wet deposition. (europa.eu)
- This study aimed to evaluate the effect of an antimicrobial chemotherapy employing H2O2 photolysis-developed to treat peri-implantitis-on biofilm-contaminated titanium surfaces in association with osteoblastic cell proliferation on the treated surface. (gu.se)
- The monomer radical cations, formed in neutral aqueous solutions, are a strong one-electron oxidant and are able to oxidize I - with a bimolecular rate constant value of 6.4 × 10 9 dm 3 mol -1 s -1 . (ias.ac.in)
- The method utilizes hydroxyl radicals to oxidize solvent accessible sites in macromolecules. (rsc.org)
- These radicals exist for about a microsecond and efficiently oxidize solvent-exposed protein segments, changing the protein's mass. (nature.com)
- Our studies suggest that the antioxidant properties of CGA may involve a direct scavenging effect of CGA on *OH. (cdc.gov)
- Previous studies have demonstrated that some of those analogues have higher antioxidant activity than curcumin [ 5 , 9 ]. (mdpi.com)
- For chard varieties that do contain betalains, recent studies have reconfirmed the antioxidant and anti-inflammatory properties of these phytonutrients. (whfoods.org)
- In this study we examine cholinesterase inhibitory activity as well as antioxidant activity of dried fruit extract including crude methyl extract and its sub fractions (Chloroform fraction, Petroleum ether fraction, Ethyl acetate fraction and aqueous fraction) of A. augusta. (omicsonline.org)
- The present study was carried out to investigate the antioxidant potential, total flavonoid and phenolic content in extracts of aerial parts of Cordia retua(Vahl. (ijpsonline.com)
- Hence the present study was designed to estimate the antioxidant activity of ethyl acetate and ethanol extracts of aerial parts of Cordia retusa (Vahl. (ijpsonline.com)
- Efficacy of taurine and hypotaurine as hydroxyl radical scavengers. (cdc.gov)
- both of which may act as radical scavengers in the cell. (cdc.gov)
- The rate constant for hypotaurine is comparable with other efficient cellular OH radical scavengers, such as ascorbate and glutathione. (cdc.gov)
- s(-1), suggesting that they are effective hydroxyl radical scavengers. (lzu.edu.cn)
- In a recent study, scientists have shown that organic cultivation of Swiss chard can help avoid this unwanted outcome. (whfoods.org)
- These study findings are consistent with the approach to food quality that we have taken on our website since day one: we encourage consumption of certified organic foods (or their equivalent) whenever possible. (whfoods.org)
- The chemical reaction generated by this solution creates a hydroxyl radical, which is extremely effective in oxidizing complex organic compounds. (environmental-expert.com)
- The hydroxyl radicals are observed to react with thioanisole (TA) with a bimolecular rate constant of 3.5 × 10 9 dm 3 mol -1 s -1 and form transient optical absorption bands with λ max = 310, 365, 530, and 740 nm. (ias.ac.in)
- OH radicals are inferred to react with thioanisole by electron transfer and 27% by OH-adduct formation. (ias.ac.in)
- The bacterioruberin radical produced is less reactive than the initial radical, and will likely react with another radical, resulting in termination of the radical chain reaction. (wikipedia.org)
- Electron spin resonance (ESR) was used to investigate the reoction of taurine and hypotaurine with hydroxyl radicals (OH). (cdc.gov)
- Future studies are needed to investigate this further, Frankenberg says. (eurekalert.org)
- Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. (hindawi.com)
- It was originally coined in reference to the use of limited proteolysis to investigate contact sites within a monoclonal antibody - protein antigen complex and a year later to examine the protection from hydroxyl radical cleavage conferred by a protein bound to DNA within a DNA-protein complex. (wikipedia.org)
- However, the inhibitory effects of CGA on specific radical species are not well understood. (cdc.gov)
- Reaction of hydroxyl radical with phenylpropanoid glycosides from Pedicularis species: A pulse radiolysis study[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1996,39(2):154-158. (lzu.edu.cn)
- OH radical is one of the main chemical species controlling the oxidizing capacity of the global Earth atmosphere. (wikipedia.org)
- However, to verify the findings of this study in relation to clinical settings, assessment using a more clinically relevant multi-species biofilm model is necessary. (gu.se)
- Hydroxyl radical footprinting (HRF) has been successfully used to study the structure of both nucleic acids and proteins. (rsc.org)
- A wealth of radiation damage studies for nucleic acids has been provided by a strong community of radiation chemists, and mechanisms have been deduced from experiments on individual nucleotides in isolation through to irradiation of whole cells and tissues. (iucr.org)
- The field is relatively new, particularly for protein studies, with only a few publications on the development and application of HRF on live cells. (rsc.org)
- Time-resolved hydroxyl radical protein footprinting employing mass spectrometry analysis was developed in the late 1990s in synchrotron radiolysis studies. (wikipedia.org)
- This method was the first employed to apply protein footprinting to the study of a protein complex. (wikipedia.org)
- By adjusting the time for radiolysis or which protein ions spend in the discharge source, a time-resolved approach is possible which is valuable for the study of protein dynamics. (wikipedia.org)
- RP-MS/Protein footprinting studies of protein complexes can also employ computational approaches to assist with this modeling. (wikipedia.org)
- Previous studies have shown that the PDX1.3 gene (encoding a key enzyme in vitamin B 6 biosynthesis) is up-regulated by UV-B. We showed that this up-regulation also occurs at the protein level in UV-B exposed Col-0 wild-type plants. (diva-portal.org)
- In order to study the role of G-proteins in UV-B signal transduction pathways, UV-B irradiated G-protein mutants of Arabidopsis thaliana were studied. (diva-portal.org)
- Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. (iucr.org)
- When it comes to protein-folding studies, mass spectrometry can provide much structural information. (nature.com)
- 2 now report a solution to this problem in their study of the submillisecond folding of the barstar protein, published in the Journal of the American Chemical Society . (nature.com)
- Figure 1: Improving the time resolution of mass spectrometry in protein-folding studies. (nature.com)
- The hydroxyl radical is often referred to as the "detergent" of the troposphere because it reacts with many pollutants, decomposing them through "cracking", often acting as the first step to their removal. (wikipedia.org)
- For instance methane, which reacts relatively slowly with hydroxyl radical, has an average lifetime of >5 years and many CFCs have lifetimes of 50 years or more. (wikipedia.org)
- Although the scavenging activity of alaternin on hydroxyl radical was similar to that of emodin in dose-dependent patterns, the inhibitory activity exhibited by the former on hydroxyl radical generation was stronger than that of the latter, with IC50 values of 3.05 +/- 0.26 microM and 13.29 +/- 3.20 microM, respectively. (nih.gov)
- Hydroxyl radical generation by coal mine dust: possible implication to coal workers' pneumoconiosis (CWP). (cdc.gov)
- The potential role of hydroxyl radical generation by bituminous coal mine dust in the development of coal workers' pneumoconiosis (CWP) was examined. (cdc.gov)
- the concentration of surface iron in coal mine dusts appears to be involved in the generation of hydroxyl radicals and play an important role in the development of CWP. (cdc.gov)
- Here we studied the photo-induced generation of 1O2 and ·OH in different sewage treatment plant units. (deepdyve.com)
- The generation of hydroxyl radical (HO·) in the presence of FePCH was observed. (asm.org)
- Instead, the most likely explanation has less to do with methane emissions and more to do with changes in the availability of the hydroxyl (OH) radical, which breaks down methane in the atmosphere. (eurekalert.org)
- The researchers also would like to see the trends they detected verified with a more detailed study of both methane sources and sinks. (eurekalert.org)
- The hydroxyl radical scavenging activity was determined by electron spin resonance spectroscopy using 5,5-dimethy-1-pyrroline-N-oxide as hydroxyl radicals trapping agents. (nih.gov)
- In the photoinduced charge injection or electron transfer process in DNA, it is generally assumed that photoexcitation induces a charge separation between the intercalated photosensitizer and an adjacent guanine base, leading to a contact radical ion pair as the crucial intermediate ( 17 - 21 ). (sciencemag.org)
- Their study shows that nitrous acid is formed in fertilized soil and released to the atmosphere, whereby the amount increases with increasing soil acidity. (mpg.de)
- In the air, nitrous acid leads to the formation of hydroxyl radicals oxidizing pollutants that then can be washed out. (mpg.de)
- Now, researchers at the Max Planck Institute in Mainz and colleagues in Beijing have discovered the origin of a bulk part of the nitrous acid that is acting beside ozone as a source of hydroxyl radicals. (mpg.de)
- Formation of superoxide and hydroxyl radicals from 1-methyl-4-phenylpyridinium ion (MPP+): reductive activation by NADPH cytochrome P-450 reductase. (semanticscholar.org)
- therefore, most of the copper-mediated hydroxyl radical formation occurs in this compartment and away from the DNA. (asm.org)
- Neutrophil degranulation inhibits potential hydroxyl-radical formation. (biochemj.org)
- All but two of the studies indicated that carnosine can prevent the formation of AGEs. (deepdyve.com)
- The hydroxyl radical spin trap molecule was 5,5-dimethyl-1-pyrroline-N-oxide. (cdc.gov)
- 2004). Another molecule from this group is lycopene , results of studies are mixed but the data from The Health Professionals Follow-up Study indicate a reduced risk of prostate cancer (Giovannucci, Liu, Platz, Stampfer, & Willett, 2007). (longecity.org)
- Other animal studies of Alzheimer's disease also suggest that curcumin may reduce levels of amyloid and oxidized proteins and prevent cognitive deficits. (sigmaaldrich.com)
- Mouse studies that evaluated the effects of dietary curcumin on inflammation, oxidative damage, and plaque pathology demonstrated that both low and high doses of curcumin significantly lowered oxidized proteins and interleukin-1beta, which is a proinflammatory cytokine elevated in the brains of these mice. (sigmaaldrich.com)