Haptens: Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.Dinitrobenzenes: Benzene derivatives which are substituted with two nitro groups in the ortho, meta or para positions.Dermatitis, Contact: A type of acute or chronic skin reaction in which sensitivity is manifested by reactivity to materials or substances coming in contact with the skin. It may involve allergic or non-allergic mechanisms.Antibodies, Catalytic: Antibodies that can catalyze a wide variety of chemical reactions. They are characterized by high substrate specificity and share many mechanistic features with enzymes.Dinitrophenols: Organic compounds that contain two nitro groups attached to a phenol.Oxazolone: Immunologic adjuvant and sensitizing agent.Dinitrofluorobenzene: Irritants and reagents for labeling terminal amino acid groups.NitrobenzenesBinding Sites, Antibody: Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.Trinitrobenzenes: Benzene derivatives which are substituted with three nitro groups in any position.Nitrohydroxyiodophenylacetate: Also called 4-hydroxy-3-iodo-5-nitrophenylacetate. A haptenic determinant that can be radiolabeled and used as salts and derivatives for investigations of immunogenic specificity studies.gamma-Globulins: Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.Antigen-Antibody Reactions: The processes triggered by interactions of ANTIBODIES with their ANTIGENS.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.p-Azobenzenearsonate: A hapten capable of eliciting both antibody formation and delayed hypersensitivity when bound to aromatic amino acids, polypeptides or proteins. It is used as an immunologic research tool.Antibodies, Bispecific: Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Myeloma Proteins: Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.NitrophenolsAntibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.HemocyaninPhenylacetates: Derivatives of phenylacetic acid. Included under this heading are a variety of acid forms, salts, esters, and amides that contain the benzeneacetic acid structure. Note that this class of compounds should not be confused with derivatives of phenyl acetate, which contain the PHENOL ester of ACETIC ACID.Antibody-Producing Cells: Cells of the lymphoid series that can react with antigen to produce specific cell products called antibodies. Various cell subpopulations, often B-lymphocytes, can be defined, based on the different classes of immunoglobulins that they synthesize.Hemolytic Plaque Technique: A method to identify and enumerate cells that are synthesizing ANTIBODIES against ANTIGENS or HAPTENS conjugated to sheep RED BLOOD CELLS. The sheep red blood cells surrounding cells secreting antibody are lysed by added COMPLEMENT producing a clear zone of HEMOLYSIS. (From Illustrated Dictionary of Immunology, 3rd ed)Serum Albumin, Bovine: Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)Epitopes: Sites on an antigen that interact with specific antibodies.Antigens: Substances that are recognized by the immune system and induce an immune reaction.Azo CompoundsImmunoglobulin Fab Fragments: Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.Immunization: Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).Antibody Affinity: A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.Mice, Inbred BALB CCross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.Dinitrochlorobenzene: A skin irritant that may cause dermatitis of both primary and allergic types. Contact sensitization with DNCB has been used as a measure of cellular immunity. DNCB is also used as a reagent for the detection and determination of pyridine compounds.Langerhans Cells: Recirculating, dendritic, antigen-presenting cells containing characteristic racket-shaped granules (Birbeck granules). They are found principally in the stratum spinosum of the EPIDERMIS and are rich in Class II MAJOR HISTOCOMPATIBILITY COMPLEX molecules. Langerhans cells were the first dendritic cell to be described and have been a model of study for other dendritic cells (DCs), especially other migrating DCs such as dermal DCs and INTERSTITIAL DENDRITIC CELLS.Irritants: Drugs that act locally on cutaneous or mucosal surfaces to produce inflammation; those that cause redness due to hyperemia are rubefacients; those that raise blisters are vesicants and those that penetrate sebaceous glands and cause abscesses are pustulants; tear gases and mustard gases are also irritants.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Penicillin G: A penicillin derivative commonly used in the form of its sodium or potassium salts in the treatment of a variety of infections. It is effective against most gram-positive bacteria and against gram-negative cocci. It has also been used as an experimental convulsant because of its actions on GAMMA-AMINOBUTYRIC ACID mediated synaptic transmission.Penicillins: A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)Butylene Glycols: 4-carbon straight chain aliphatic hydrocarbons substituted with two hydroxyl groups. The hydroxyl groups cannot be on the same carbon atom.Penicillin V: A broad-spectrum penicillin antibiotic used orally in the treatment of mild to moderate infections by susceptible gram-positive organisms.Solid-Phase Synthesis Techniques: Techniques used to synthesize chemicals using molecular substrates that are bound to a solid surface. Typically a series of reactions are conducted on the bound substrate that results in either the covalent attachment of specific moieties or the modification of existing function groups. These techniques offer an advantage to those involving solution reactions in that the substrate compound does not have to be isolated and purified between the reaction steps.Environmental Policy: A course of action or principle adopted or proposed by a government, party, business, or individual that concerns human interactions with nature and natural resources.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Resins, Plant: Flammable, amorphous, vegetable products of secretion or disintegration, usually formed in special cavities of plants. They are generally insoluble in water and soluble in alcohol, carbon tetrachloride, ether, or volatile oils. They are fusible and have a conchoidal fracture. They are the oxidation or polymerization products of the terpenes, and are mixtures of aromatic acids and esters. Most are soft and sticky, but harden after exposure to cold. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)T-Lymphocytes, Helper-Inducer: Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Trinitrobenzenesulfonic Acid: A reagent that is used to neutralize peptide terminal amino groups.Dihydroorotate Oxidase: An enzyme that in the course of pyrimidine biosynthesis, catalyzes the oxidation of dihydro-orotic acid to orotic acid utilizing oxygen as the electron acceptor. This enzyme is a flavoprotein which contains both FLAVIN-ADENINE DINUCLEOTIDE and FLAVIN MONONUCLEOTIDE as well as iron-sulfur centers. EC 1.3.3.1.Colitis: Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.Colon: The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.Lymphocytes: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.Thenoyltrifluoroacetone: Chelating agent and inhibitor of cellular respiration.Enema: A solution or compound that is introduced into the RECTUM with the purpose of cleansing the COLON or for diagnostic procedures.
  • of the binding of Fab' fragments to small haptens have shown that the cross-linking constant (the equilibrium constant for binding an additional Fab' fragment to a hapten-Fab' complex) is strongly dependent on the length of the hapten. (osti.gov)
  • A typical PRIT protocol begins with a cold dose of bispecific antibody (BsAb) to prelocalize at the tumor, followed with a clearing agent (CA) to remove circulating off-target antibody and then last, a rapidly clearing radiolabeled small-molecule hapten or peptide. (aacrjournals.org)
  • Results of this study were presented by William McBride, Ph.D., Senior Director, Peptide Chemistry. (cnbc.com)
  • Their new study evaluated processes aimed at optimizing the radiolabeling procedure for multi-dose preparation of IMP485, a hapten-peptide used for pretargeting, a highly sensitive and specific method for targeting cancers using a bispecific antibody. (cnbc.com)
  • Heroin-HIV-1 (H2) vaccine: induction of dual immunologic effects with a heroin hapten-conjugate and an HIV-1 envelope V2 peptide with liposomal lipid A as an adjuvant. (drugabuse.gov)
  • For these studies, infectious clones of embryo-invading plant RNA viruses will be modified to express bacterial genes and guide nucleic acids for genome editing. (usda.gov)
  • T cell mitogen response assays, one-way mixed lymphocyte reactions, and bacterial phagocytosis and killing by spleen and peripheral blood cells will be studied, and numbers of B, T, and adherent cells in spleen, marrow, thymus, and peripheral blood will be determined. (epa.gov)
  • We established that the spacer length played a significant role in hapten display within the bacterial cell surface," the article's authors detailed, "a result that was confirmed both experimentally and via molecular dynamics simulations. (genengnews.com)
  • In vitro assay for hapten-specific priming of human T lymphocytes. (cdc.gov)
  • Based on the distinct sensitizing properties of haptens as compared to irritants we propose to develop an in vitro assay that may help identify potential contact sensitizers and irritants as well as individuals susceptible to developing ACD or ICD. (cdc.gov)
  • Thus this project can help (a) refine an in vitro assay for future application in clinical laboratory for screening various potential haptens and irritants as well as susceptible individuals and (b) provide better understanding of cellular events that lead to allergic Vs. irritant contact dermatitis. (cdc.gov)
  • We conducted studies to determine the magnitude and sources of variability in androgen assay results and to identify laboratories capable of performing such assays for large epidemiological studies. (aacrjournals.org)
  • The results for ADIOL suggest that this assay is not yet ready for use in epidemiological studies. (aacrjournals.org)
  • We have estimated assay variability over the time required to assay samples from a large epidemiological study by using four measurements spaced over 3 months. (aacrjournals.org)
  • The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin. (ebscohost.com)
  • Presents a study which developed an in-house enzyme-linked immunosorbent assay (ELISA) for the quantification of human albumin in urine samples. (ebscohost.com)
  • The article presents a study regarding the use of enzyme-linked immunosorbent assay (ELSIA) in detecting triasulfuron in soils. (ebscohost.com)
  • Sensitization to haptens is characterized by the activation of dendritic cells (DCs), which migrate to the skin-draining lymph nodes, and by the priming of allergen specific T cells. (frontiersin.org)
  • Initial studies of hapten-based murine colitis revealed extensive mucosal hypoxia and concomitant HIF-1 activation during colitis. (jci.org)
  • Studies of colitis in these mice revealed that decreased HIF-1 expression correlated with more severe clinical symptoms (mortality, weight loss, colon length), while increased HIF levels were protective in these parameters. (jci.org)
  • The aim of this study was to evaluate the in vivo effects of Vido on IL‑17 expression and colitis symptoms, in the presence or absence of concomitant uridine (Ur) dosing, by using our rat model of hapten-induced colitis (Fitzpatrick et al. (ecco-ibd.eu)
  • The hapten-enhanced clinical effect of UMIPIC conferred a superior survival time in patients with advanced lung cancer compared with ITCT. (dovepress.com)
  • In December 2019, the company reported that EB01 had demonstrated positive safety data in healthy volunteers participating in its ongoing phase IIb clinical study in chronic ACD. (yahoo.com)
  • We are enthusiastic that we are able to begin clinical development of a first-in-class compound that can potentially prevent contact dermatitis, associated medical treatments and lost time at work,' stated Raymond J. Hage, Jr., Hapten Science's CEO. (fiercepharma.com)
  • DATATRAK International, Inc., the leader in developing cloud-based, unified dClinical technologies and delivering related services for the clinical trials industry, today announced that Cassiopea S.p.A., the newly IPO'd former subsidiary of longtime partner Cosmo Pharmaceuticals SA has selected DATATRAK for a significant global study. (news-medical.net)
  • Based on published meta-analyses of randomized clinical studies, we know that the mean wart cure rate for placebo is around 23%, and it is not much higher for daily use of topical salicylic acid for 3 to 6 months, which is currently the recommended first-line therapy. (prnewswire.com)
  • Currently my major focus is on clinical care of patients with liver disease and I am engaged in studies of the role of NAFLD (non-alcoholic liver disease) in patients with hepatits C and in post liver transplant patients. (stanford.edu)
  • Because of its diverse clinical presentations, DIH is difficult to study in patients. (jove.com)
  • A human genetic theory of infectious diseases has emerged gradually from clinical and epidemiological studies, building on many elegant studies in plants and animals. (pnas.org)
  • While in vivo studies can cause undue pain and distress in mice when done improperly, the advantage of an in vivo model is the ability to evaluate the pathogenesis of disease in a large number of mice. (jove.com)
  • Thus, we will demonstrate our model protocol and how it can be utilized to study in vivo and in vitro mechanisms of DIH. (jove.com)
  • Conflicting results have been obtained regarding pathogenic versus protective roles of MCs in CHS, and this has been attributed in part to the limitations of certain models for studying MC functions in vivo . (frontiersin.org)
  • Other haptens that are commonly used in molecular biology applications include fluorescein, biotin, digoxigenin, and dinitrophenol. (wikipedia.org)
  • As an alternative to streptavidin/biotin, other high-affinity antibody-hapten pairs have been developed for PRIT ( 2 ). (aacrjournals.org)
  • One advantage of using fluorescent dyes as haptens instead of biotin is that the hapten signal is usually visible, or at least its concentration can be measured by its absorption in solution, preceding the secondary detection step. (thermofisher.com)
  • To raise the polyclonal antibody, 0.5 mg of caproyl pyrraline conjugated KLH in 50% Freund's complete adjuvant was injected intradermally into a rabbit at 20 skin sites, followed by four booster injections with the same amount of hapten conjugated KLH in 50% Freund's incomplete adjuvant. (bmj.com)
  • The objective of the study reported here was to evaluate the therapeutic effects of hapten-enhanced chemoimmunotherapy in the treatment of advanced lung cancer by ultra-minimum incision personalized intratumoral chemoimmunotherapy (UMIPIC) and to analyze the effect of this immune booster. (dovepress.com)
  • UMIPIC was delivered intratumorally in combination with a proprietary therapeutic regimen composed of three components - an oxidant, a cytotoxic drug, and hapten. (dovepress.com)
  • RXi Pharmaceuticals Corporation, a biotechnology company focused on discovering and developing innovative therapies addressing high-unmet medical needs, today announced the closing of its previously disclosed exclusive global license agreement for the therapeutic use of Samcyprone with Hapten Pharmaceuticals, LLC. (news-medical.net)
  • The immunotherapeutic response rate - a prerequisite for therapeutic response - was 97.7% across all 88 subjects enrolled in the study. (prnewswire.com)
  • These studies are intended to be conducted during 2016. (fiercepharma.com)
  • 2016). Some studies have shown its effect on 17-alpha-ethinyl estradiol (E4) are other estrogens which uterine cancer, ovary and other cancers (Yi et al. (deepdyve.com)
  • In contrast, once weekly treatments with Samcyprone were used in our study, and of the warts that cleared, most did so within the first 10 weeks of treatment. (prnewswire.com)
  • In our Samcyprone study there was no such requirement for wart lesion preparation, which otherwise may have resulted in even better wart clearance rates. (prnewswire.com)
  • Based on the exciting study results with this new proprietary formulation of DPCP, I believe that Samcyprone will offer patients not only a powerful new treatment option, but also one that is more patient friendly than pharmacy compounded products and other currently used treatments that require more frequent applications and longer treatments. (prnewswire.com)
  • The haptens were potent inhibitors of this esterolytic activity, in agreement with their classification as transition state analogs. (sciencemag.org)
  • The objective of this study is to study effects of in ovo treatment with putative endocrine disruptors on basic measures of specific and nonspecific immune functions and on host resistance to a natural infectious disease of alligators, which will provide a stringent assessment of the biological significance of alterations in measures of immunity. (epa.gov)
  • T cell- dependent immunity was assessed by studying the humoral response to the hapten oxazolone. (docme.ru)
  • GSK today announced updated results for Tafinlar (dabrafenib) from a planned analysis of the phase III BREAK-3 study in 250 patients with BRAF V600E mutant metastatic melanoma. (news-medical.net)
  • Secondary occurrence of either malignant melanoma or squamous cell skin cancer in cohorts of patients with a first diagnosis of either non-Hodgkin's lymphoma or chronic lymphocytic leukaemia, and vice versa, were studied. (bmj.com)
  • Dr. Tsai?s research focuses on studies that are designed to understand the regulation of mast cell and basophil development and to elucidate the roles of these cells in health and disease. (stanford.edu)
  • This ligand is a stable trimer of peptides derived from bacteriophage T4 fibritin, each conjugated to a hapten (DNP). (jove.com)
  • Although the haptens are well known, the chemical reactions of haptens inducing ACD is not well known. (openthesis.org)
  • A previous study reported that IgE and TARC, which are elevated in AD, were induced by the chemical hapten, DNCB (Choi et al. (thefreedictionary.com)
  • Roudbari and Rezakazemi AMB Expr (2018) 8:91 Page 2 of 8 researchers and national and regional authorities to them elastic skin and reduction of organic chemical pollutants and several studies have been conducted to determine from liquid environments (Mahravan et al. (deepdyve.com)
  • Virtually every study agrees that the prevalence of COAG increases with age of the population being considered.Diabetes is also reported to be a risk factor for COAG in several studies, although further information from population based studies is necessary to definitely prove the role of diabetes in the development of COAG. (bmj.com)
  • Study record managers: refer to the Data Element Definitions if submitting registration or results information. (clinicaltrials.gov)
  • Eisai Inc. announced today the results of its Phase 3 trial (Study 309), which showed that eribulin met the study's primary endpoint evaluating overall survival in patients who had advanced leiomyosarcoma (LMS) or adipocytic sarcoma (ADI), two types of advanced soft tissue sarcoma. (news-medical.net)
  • The study results show furthermore that the product was safe and well tolerated. (prnewswire.com)
  • The long-term objective of our studies is to uncover mechanisms responsible for the development of chronic liver inflammation and injury by studying DIH in susceptible patients. (jove.com)
  • Previous studies have implicated hypoxia in mucosal tissue damage resulting from both acute and chronic inflammation. (jci.org)
  • MAb 18B7 was shown by immunofluorescence and agglutination studies to bind to all four serotypes of C. neoformans , opsonize C. neoformans serotypes A and D, enhance human and mouse effector cell antifungal activity, and activate the complement pathway leading to deposition of complement component 3 (C3) on the cryptococcal capsule. (asm.org)
  • These include the ovarian and adrenal androgens previously analyzed in epidemiological studies of breast cancer, as well as other androgen metabolites of potential importance in breast cancer etiology. (aacrjournals.org)
  • AbbVie, a global biopharmaceutical company, today announced findings from a Phase 2 study of the investigational medicine veliparib combined with the chemotherapy regimen carboplatin and paclitaxel that showed an improvement in median progression-free survival (PFS) in patients with previously untreated metastatic or advanced non-small cell lung cancer (NSCLC) who are current smokers. (news-medical.net)
  • Previously my basci research laboratory studied the hepatic maetaoblism of these substances in animal and cellular models. (stanford.edu)
  • The study of pathogenic mechanisms involved in the development of anesthetic and any form of DIH has been previously hampered by the lack of an animal model that closely mimics the induction of human disease. (jove.com)
  • The approach to equilibrium in such systems has been previously studied. (illinois.edu)
  • A hydrolyzed piperacillin hapten was detected on four lysine residues of human serum albumin (HSA) isolated from tolerant patients. (jimmunol.org)