Stromal Cells: Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Endometrium: The mucous membrane lining of the uterine cavity that is hormonally responsive during the MENSTRUAL CYCLE and PREGNANCY. The endometrium undergoes cyclic changes that characterize MENSTRUATION. After successful FERTILIZATION, it serves to sustain the developing embryo.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Corneal Stroma: The lamellated connective tissue constituting the thickest layer of the cornea between the Bowman and Descemet membranes.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Decidua: The hormone-responsive glandular layer of ENDOMETRIUM that sloughs off at each menstrual flow (decidua menstrualis) or at the termination of pregnancy. During pregnancy, the thickest part of the decidua forms the maternal portion of the PLACENTA, thus named decidua placentalis. The thin portion of the decidua covering the rest of the embryo is the decidua capsularis.Chemokine CXCL12: A CXC chemokine that is chemotactic for T-LYMPHOCYTES and MONOCYTES. It has specificity for CXCR4 RECEPTORS. Two isoforms of CXCL12 are produced by alternative mRNA splicing.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Sarcoma, Endometrial Stromal: A highly malignant subset of neoplasms arising from the endometrial stroma. Tumors in this group infiltrate the stroma with a wide range of atypia cells and numerous mitoses. They are capable of widespread metastases (NEOPLASM METASTASIS).Hematopoiesis: The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Cell Communication: Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Multipotent Stem Cells: Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Endometriosis: A condition in which functional endometrial tissue is present outside the UTERUS. It is often confined to the PELVIS involving the OVARY, the ligaments, cul-de-sac, and the uterovesical peritoneum.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Mice, Inbred C57BLParacrine Communication: Cellular signaling in which a factor secreted by a cell affects other cells in the local environment. This term is often used to denote the action of INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS on surrounding cells.Uterus: The hollow thick-walled muscular organ in the female PELVIS. It consists of the fundus (the body) which is the site of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus, are the FALLOPIAN TUBES.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Embryo Implantation: Endometrial implantation of EMBRYO, MAMMALIAN at the BLASTOCYST stage.Colony-Forming Units Assay: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Receptors, CXCR4: CXCR receptors with specificity for CXCL12 CHEMOKINE. The receptors may play a role in HEMATOPOIESIS regulation and can also function as coreceptors for the HUMAN IMMUNODEFICIENCY VIRUS.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Tumor Microenvironment: The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.Proto-Oncogene Proteins c-kit: A protein-tyrosine kinase receptor that is specific for STEM CELL FACTOR. This interaction is crucial for the development of hematopoietic, gonadal, and pigment stem cells. Genetic mutations that disrupt the expression of PROTO-ONCOGENE PROTEINS C-KIT are associated with PIEBALDISM, while overexpression or constitutive activation of the c-kit protein-tyrosine kinase is associated with tumorigenesis.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Prostate: A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Chemokines, CXC: Group of chemokines with paired cysteines separated by a different amino acid. CXC chemokines are chemoattractants for neutrophils but not monocytes.Menstrual Cycle: The period from onset of one menstrual bleeding (MENSTRUATION) to the next in an ovulating woman or female primate. The menstrual cycle is regulated by endocrine interactions of the HYPOTHALAMUS; the PITUITARY GLAND; the ovaries; and the genital tract. The menstrual cycle is divided by OVULATION into two phases. Based on the endocrine status of the OVARY, there is a FOLLICULAR PHASE and a LUTEAL PHASE. Based on the response in the ENDOMETRIUM, the menstrual cycle is divided into a proliferative and a secretory phase.Connective Tissue Cells: A group of cells that includes FIBROBLASTS, cartilage cells, ADIPOCYTES, smooth muscle cells, and bone cells.Interleukin-7: A cytokine produced by bone marrow stromal cells that promotes the growth of B-LYMPHOCYTE precursors and is co-mitogenic with INTERLEUKIN-2 for mature T-LYMPHOCYTE activation.Cornea: The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Cell Adhesion: Adherence of cells to surfaces or to other cells.Thymus Gland: A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Adipogenesis: The differentiation of pre-adipocytes into mature ADIPOCYTES.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Cell Line, Tumor: A cell line derived from cultured tumor cells.Cell Lineage: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.Medroxyprogesterone Acetate: A synthetic progestin that is derived from 17-hydroxyprogesterone. It is a long-acting contraceptive that is effective both orally or by intramuscular injection and has also been used to treat breast and endometrial neoplasms.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 188.8.131.52.Epithelium: One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.Mice, SCID: Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.Cell SeparationBlotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Interleukin-6: A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.Bone Marrow Transplantation: The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.Adipose Tissue: Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.Osteoclasts: A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.Connective Tissue: Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Mice, Inbred BALB CB-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Progesterone: The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.Insulin-Like Growth Factor Binding Protein 1: One of the six homologous proteins that specifically bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions. The function of this protein is not completely defined. However, several studies demonstrate that it inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions. (Proc Soc Exp Biol Med 1993;204(1):4-29)Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Transforming Growth Factor beta: A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Lymphotoxin beta Receptor: A member of the tumor necrosis factor receptor superfamily. It has specificity for LYMPHOTOXIN ALPHA1, BETA2 HETEROTRIMER and TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 14. The receptor plays a role in regulating lymphoid ORGANOGENESIS and the differentiation of certain subsets of NATURAL KILLER T-CELLS. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.Chemokine CXCL13: A CXC chemokine that is chemotactic for B-LYMPHOCYTES. It has specificity for CXCR5 RECEPTORS.Estradiol: The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Interleukin-11: A lymphohematopoietic cytokine that plays a role in regulating the proliferation of ERYTHROID PRECURSOR CELLS. It induces maturation of MEGAKARYOCYTES which results in increased production of BLOOD PLATELETS. Interleukin-11 was also initially described as an inhibitor of ADIPOGENESIS of cultured preadipocytes.Tissue Engineering: Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.RANK Ligand: A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. It plays an important role in regulating OSTEOCLAST differentiation and activation.Multiple Myeloma: A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.Vascular Endothelial Growth Factor A: The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Immunophenotyping: Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.Mesonephros: One of a pair of excretory organs (mesonephroi) which grows caudally to the first pair (PRONEPHROI) during development. Mesonephroi are the permanent kidneys in adult amphibians and fish. In higher vertebrates, proneprhoi and most of mesonephroi degenerate with the appearance of metanephroi. The remaining ducts become WOLFFIAN DUCTS.Benzamides: BENZOIC ACID amides.Adipocytes: Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.Vimentin: An intermediate filament protein found in most differentiating cells, in cells grown in tissue culture, and in certain fully differentiated cells. Its insolubility suggests that it serves a structural function in the cytoplasm. MW 52,000.Chondrogenesis: The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.Stem Cell Factor: A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.Gastrointestinal Neoplasms: Tumors or cancer of the GASTROINTESTINAL TRACT, from the MOUTH to the ANAL CANAL.Transplantation, Heterologous: Transplantation between animals of different species.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Osteocytes: Mature osteoblasts that have become embedded in the BONE MATRIX. They occupy a small cavity, called lacuna, in the matrix and are connected to adjacent osteocytes via protoplasmic projections called canaliculi.Hematopoietic Cell Growth Factors: These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.Tissue Scaffolds: Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.Sex Cord-Gonadal Stromal Tumors: Neoplasms derived from the primitive sex cord or gonadal stromal cells of the embryonic GONADS. They are classified by their presumed histogenesis and differentiation. From the sex cord, there are SERTOLI CELL TUMOR and GRANULOSA CELL TUMOR; from the gonadal stroma, LEYDIG CELL TUMOR and THECOMA. These tumors may be identified in either the OVARY or the TESTIS.Bone Morphogenetic Protein 2: A potent osteoinductive protein that plays a critical role in the differentiation of osteoprogenitor cells into OSTEOBLASTS.Pyrimidines: A family of 6-membered heterocyclic compounds occurring in nature in a wide variety of forms. They include several nucleic acid constituents (CYTOSINE; THYMINE; and URACIL) and form the basic structure of the barbiturates.Epithelium, Corneal: Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.Prostatic Neoplasms: Tumors or cancer of the PROSTATE.PiperazinesMembrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Intercellular Signaling Peptides and Proteins: Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.Mesoderm: The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.Interleukin-1: A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.Receptor, Platelet-Derived Growth Factor alpha: A PDGF receptor that binds specifically to both PDGF-A chains and PDGF-B chains. It contains a protein-tyrosine kinase activity that is involved in SIGNAL TRANSDUCTION.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Wound Healing: Restoration of integrity to traumatized tissue.ADP-ribosyl Cyclase: A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Neovascularization, Physiologic: The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.Bone and Bones: A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.Fetal Blood: Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.Neovascularization, Pathologic: A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Cell- and Tissue-Based Therapy: Therapies that involve the TRANSPLANTATION of CELLS or TISSUES developed for the purpose of restoring the function of diseased or dysfunctional cells or tissues.Stem Cell Niche: A particular zone of tissue composed of a specialized microenvironment where stem cells are retained in a undifferentiated, self-renewable state.Antigens, Thy-1: A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Calcification, Physiologic: Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.Trophoblasts: Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA).Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.Corneal Opacity: Disorder occurring in the central or peripheral area of the cornea. The usual degree of transparency becomes relatively opaque.Bone Regeneration: Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.Prostatic Hyperplasia: Increase in constituent cells in the PROSTATE, leading to enlargement of the organ (hypertrophy) and adverse impact on the lower urinary tract function. This can be caused by increased rate of cell proliferation, reduced rate of cell death, or both.Endometrial Stromal Tumors: Neoplasms of the endometrial stroma that sometimes involve the MYOMETRIUM. These tumors contain cells that may closely or remotely resemble the normal stromal cells. Endometrial stromal neoplasms are divided into three categories: (1) benign stromal nodules; (2) low-grade stromal sarcoma, or endolymphatic stromal myosis; and (3) malignant endometrial stromal sarcoma (SARCOMA, ENDOMETRIAL STROMAL).Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Breast Neoplasms: Tumors or cancer of the human BREAST.Collagenases: Enzymes that catalyze the degradation of collagen by acting on the peptide bonds.Fibroblast Growth Factor 2: A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).Macrophage Colony-Stimulating Factor: A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. M-CSF is a disulfide-bonded glycoprotein dimer with a MW of 70 kDa. It binds to a specific high affinity receptor (RECEPTOR, MACROPHAGE COLONY-STIMULATING FACTOR).Cellular Microenvironment: Local surroundings with which cells interact by processing various chemical and physical signals, and by contributing their own effects to this environment.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Neoplasm Invasiveness: Ability of neoplasms to infiltrate and actively destroy surrounding tissue.Matrix Metalloproteinase 9: An endopeptidase that is structurally similar to MATRIX METALLOPROTEINASE 2. It degrades GELATIN types I and V; COLLAGEN TYPE IV; and COLLAGEN TYPE V.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Myofibroblasts: Spindle-shaped cells with characteristic CONTRACTILE PROTEINS and structures that contribute to the WOUND HEALING process. They occur in GRANULATION TISSUE and also in pathological processes such as FIBROSIS.Cell Transplantation: Transference of cells within an individual, between individuals of the same species, or between individuals of different species.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Lymphopoiesis: Formation of LYMPHOCYTES and PLASMA CELLS from the lymphoid stem cells which develop from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW. These lymphoid stem cells differentiate into T-LYMPHOCYTES; B-LYMPHOCYTES; PLASMA CELLS; or NK-cells (KILLER CELLS, NATURAL) depending on the organ or tissues (LYMPHOID TISSUE) to which they migrate.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Dinoprostone: The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.Antigens, CD146: A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Gelatinases: A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-.Antigens, Differentiation: Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Osteoprotegerin: A secreted member of the TNF receptor superfamily that negatively regulates osteoclastogenesis. It is a soluble decoy receptor of RANK LIGAND that inhibits both CELL DIFFERENTIATION and function of OSTEOCLASTS by inhibiting the interaction between RANK LIGAND and RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Cell Adhesion Molecules: Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.Vascular Cell Adhesion Molecule-1: Cytokine-induced cell adhesion molecule present on activated endothelial cells, tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, and myotubes. It is important for the recruitment of leukocytes to sites of inflammation. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, p154)Lymphoid Tissue: Specialized tissues that are components of the lymphatic system. They provide fixed locations within the body where a variety of LYMPHOCYTES can form, mature and multiply. The lymphoid tissues are connected by a network of LYMPHATIC VESSELS.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Receptor Activator of Nuclear Factor-kappa B: A tumor necrosis factor receptor family member that is specific for RANK LIGAND and plays a role in bone homeostasis by regulating osteoclastogenesis. It is also expressed on DENDRITIC CELLS where it plays a role in regulating dendritic cell survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Mice, Inbred NOD: A strain of non-obese diabetic mice developed in Japan that has been widely studied as a model for T-cell-dependent autoimmune insulin-dependent diabetes mellitus in which insulitis is a major histopathologic feature, and in which genetic susceptibility is strongly MHC-linked.Parietal Bone: One of a pair of irregularly shaped quadrilateral bones situated between the FRONTAL BONE and OCCIPITAL BONE, which together form the sides of the CRANIUM.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Keratitis, Herpetic: A superficial, epithelial Herpesvirus hominis infection of the cornea, characterized by the presence of small vesicles which may break down and coalesce to form dendritic ulcers (KERATITIS, DENDRITIC). (Dictionary of Visual Science, 3d ed)Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Stromal cells mediate retinoid-dependent functions essential for renal development. (1/4669)The essential role of vitamin A and its metabolites, retinoids, in kidney development has been demonstrated in vitamin A deficiency and gene targeting studies. Retinoids signal via nuclear transcription factors belonging to the retinoic acid receptor (RAR) and retinoid X receptor (RXR) families. Inactivation of RARaplpha and RARbeta2 receptors together, but not singly, resulted in renal malformations, suggesting that within a given renal cell type, their concerted function is required for renal morphogenesis. At birth, RARalpha beta2(-) mutants displayed small kidneys, containing few ureteric bud branches, reduced numbers of nephrons and lacking the nephrogenic zone where new nephrons are continuously added. These observations have prompted us to investigate the role of RARalpha and RARbeta2 in renal development in detail. We have found that within the embryonic kidney, RARalpha and RARbeta2 are colocalized in stromal cells, but not in other renal cell types, suggesting that stromal cells mediate retinoid-dependent functions essential for renal development. Analysis of RARalpha beta2(-) mutant kidneys at embryonic stages revealed that nephrons were formed and revealed no changes in the intensity or distribution of molecular markers specific for different metanephric mesenchymal cell types. In contrast the development of the collecting duct system was greatly impaired in RARalpha beta2(-) mutant kidneys. Fewer ureteric bud branches were present, and ureteric bud ends were positioned abnormally, at a distance from the renal capsule. Analysis of genes important for ureteric bud morphogenesis revealed that the proto-oncogene c-ret was downregulated. Our results suggest that RARalpha and RARbeta2 are required for generating stromal cell signals that maintain c-ret expression in the embryonic kidney. Since c-ret signaling is required for ureteric bud morphogenesis, loss of c-ret expression is a likely cause of impaired ureteric bud branching in RARalpha beta2(-) mutants. (+info)
Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. (2/4669)OBJECTIVE: To investigate the features of synovial stromal cells established from patients with rheumatoid arthritis (RA), and to define these cells as nurse cells. METHODS: Synovial nurse-like stromal cell lines (RA-SNCs) were established from patients with RA. These cell lines were examined for morphology, pseudoemperipolesis activity, cell surface markers, and cytokine production. The interaction between these RA-SNCs and a synovial tissue B cell clone was also examined. RESULTS: RA-SNCs had nurse cell activity. They spontaneously produced interleukin-6 (IL-6), IL-8, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Furthermore, they produced IL-1beta and tumor necrosis factor alpha and expressed higher levels of the other cytokines after coculture with the B cell clone. Proliferation and Ig production by the B cell clone were dependent on direct contact with RA-SNCs. CONCLUSION: These results indicate that the RA-SNCs were nurse cells. The findings suggest that RA-SNCs may play an important role in the pathogenesis of RA by producing large amounts of cytokines and maintaining infiltrating lymphocytes. (+info)
Expression of neurotrophins and their receptors in human bone marrow. (3/4669)The expression of neurotrophins and their receptors, the low-affinity nerve growth factor receptor (p75LNGFR) and the Trk receptors (TrkA, TrkB, and TrkC), was investigated in human bone marrow from 16 weeks fetal age to adulthood. Using reverse transcription-polymerase chain reaction, all transcripts encoding for catalytic and truncated human TrkB or TrkC receptors were detected together with trkAI transcripts, whereas trkAII transcripts were found only in control nerve tissues. Transcripts for the homologue of the rat truncated TrkC(ic113) receptor were identified for the first time in human tissue. Stromal adventitial reticular cells were found immunoreactive for all neutrophin receptors. In contrast, hematopoietic cell types were not immunoreactive for p75LNGFR but showed immunoreactivity for one or several Trk receptors. TrkA immunoreactivity was found in immature erythroblasts. Catalytic TrkB immunoreactivity was observed in eosinophilic metamyelocytes and polymorphonuclear cells. Truncated TrkB immunoreactivity was found in erythroblasts and megacaryocytes. Immunoreactivity for both catalytic and truncated TrkC receptor was observed in promyelocytes, myelocytes, some polymorphonuclear cells and megacaryocytes. Neutrophin transcript levels appeared higher at fetal than at adult stages, no variation in Trk family transcript levels was observed. The local expression of neurotrophin genes suggests a wide range of paracrine and/or autocrine mode of action through their corresponding receptors within the bone marrow. (+info)
Marker molecules of human endometrial differentiation can be hormonally regulated under in-vitro conditions as in-vivo. (4/4669)An established cell culture system of isolated human endometrial stromal and epithelial cells has been used to study the effects of oestrogen and progesterone, as well as their antagonists, upon endometrial cells. Normal hormonal regulation in vivo was investigated simultaneously in endometrial tissue samples taken at different phases of the menstrual cycle. Several marker molecules analysed by immunohistochemistry appeared to depend strongly on endocrine regulation and could be traced in culture. Immunohistochemically, basic parameters of cell biology were identified in vitro, e.g. cell proliferation (Ki-67), adhesion molecules (beta3 integrin) and paracrine factors (leukaemia inhibitory factor). The most reliable parameters to assess hormonal influences were oestrogen and progesterone receptor molecules. Immunohistochemical localization could be improved by molecular biological analysis using RT-PCR. In the presence of oestrogen, a significant expression of hormone receptors was also shown by RT-PCR, and withdrawal of oestrogens and addition of gestagen, i.e. medroxyprogesterone acetate, caused receptor downregulation. Addition of the anti-oestrogen ICI 182.780 to cell-culture medium significantly decreased the synthesis of progesterone receptors. (+info)
Expression of the oxytocin receptor in relation to steroid receptors in the uterus of a primate model, the marmoset monkey. (5/4669)The dynamics of the receptors for oestrogen (ER), progesterone (PR) and oxytocin (OTR) in the marmoset uterus have been analysed throughout the entire cycle and early pregnancy. Uteri obtained during the early, mid/late and late proliferative phase, and the early, mid and late secretory phase and early pregnancy were examined by immunohistochemistry (OTR, ER, PR) and autoradiography (OTR). A massive upregulation of the ER in the cell nuclei of glandular epithelium and stromal cells during the mid proliferative phase was succeeded by a declining staining intensity and positively stained cell number in the secretory phase. PR immunoreactivity increased in the late proliferative phase and early secretory phase, mainly within the cell nuclei, and then declined in both intensity and cell number towards the mid to late secretory phase. Myometrium showed a similar staining pattern for the steroid receptors. OTR were expressed weakly in stroma throughout the entire cycle, increasing slightly in the secretory phase. Glandular epithelium showed positive staining only during the periovulatory period. Myometrial OTR expression was weak during the proliferative phase, increased towards the secretory phase, and was maximal in the late secretory phase. Myometrial tissue adjacent to endometrium was most strongly stained. A cyclic shift evidently occurred in the pattern of steroid receptors, perhaps reflecting the steroid environment or the luteinizing hormone increase associated with ovulation. (+info)
Detection of Kaposi's sarcoma herpesvirus DNA sequences in multiple myeloma bone marrow stromal cells. (6/4669)Whether Kaposi's sarcoma herpesvirus (KSHV) is associated with multiple myeloma (MM) remains controversial. We assayed for KSHV DNA sequences in long-term bone marrow stromal cells (BMSCs) from 26 patients with MM and 4 normal donors. Polymerase chain reaction (PCR) using primers which amplify a KSHV gene sequence to yield a 233-bp fragment (KS330233 within open reading frame 26) was negative in all cases. Aliquots of these PCR products were used as templates in subsequent nested PCR, with primers that amplify a 186-bp product internal to KS330233. BMSCs from 24 of 26 (92%) patients with MM and 1 of 4 normal donors were KSHV PCR+. DNA sequence analyses showed interpatient specific mutations (2 to 3 bp). Both Southern blot and sequence analyses confirmed the specificity of PCR results. The presence of the KSHV gene sequences was further confirmed by amplifying T 1.1 (open reading frame [ORF] K7) and viral cyclin D (ORF 72), two other domains within the KSHV genome. Immunohistochemical studies of KSHV PCR+ MM BMSCs demonstrate expression of dendritic cell (DC) lineage markers (CD68, CD83, and fascin). Serological studies for the presence of KSHV lytic or latent antibodies were performed using sera from 53 MM patients, 12 normal donors, and 5 human immunodeficiency virus (HIV)/KSHV+ patients. No lytic or latent antibodies were present in sera from either MM patients or normal donors. Taken together, these findings show that KSHV DNA sequences are detectable in BMSCs from the majority of MM patients, but that serologic responses to KSHV are not present. Ongoing studies are defining whether the lack of antibody response is caused by the absence of ongoing infection, the presence of a novel viral strain associated with MM, or underlying immunodeficiency in these patients. (+info)
In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. (7/4669)Recent studies have shown that long-term repopulating hematopoietic stem cells (HSCs) first appear in the aorta-gonad-mesonephros (AGM) region. Our immunohistochemistry study showed that TEK+ cells existed in the AGM region. Approximately 5% of AGM cells were TEK+, and most of these were CD34(+) and c-Kit+. We then established a coculture system of AGM cells using a stromal cell line, OP9, which is deficient in macrophage colony-stimulating factor (M-CSF). With this system, we showed that AGM cells at 10.5 days postcoitum (dpc) differentiated and proliferated into both hematopoietic and endothelial cells. Proliferating hematopoietic cells contained a significant number of colony-forming cells in culture (CFU-C) and in spleen (CFU-S). Among primary AGM cells at 10.5 dpc, sorted TEK+ AGM cells generated hematopoietic cells and platelet endothelial cell adhesion molecule (PECAM)-1(+) endothelial cells on the OP9 stromal layer, while TEK- cells did not. When a ligand for TEK, angiopoietin-1, was added to the single-cell culture of AGM, endothelial cell growth was detected in the wells where hematopoietic colonies grew. Although the incidence was still low (1/135), we showed that single TEK+ cells generated hematopoietic cells and endothelial cells simultaneously, using a single-cell deposition system. This in vitro coculture system shows that the TEK+ fraction of primary AGM cells is a candidate for hemangioblasts, which can differentiate into both hematopoietic cells and endothelial cells. (+info)
A novel stromal cell-dependent B lymphoid stem-like cell line that induces immunoglobulin gene rearrangement. (8/4669)A stroma-dependent B lymphoid cell line (B31-1) has been established by coculturing sorted stem cells on a novel bone marrow stromal cell line (TBR31-1). B31-1 cells express B220, but do not express other B lymphoid differentiation markers including CD43, heat stable antigen (HSA), or surface immunoglobulin (Ig) M (sIgM), and their Ig heavy chain (IgH) gene loci are germ-line in configuration. The addition of interleukin (IL)-7 or coculture with another stromal cell line, ST2, induces D-J rearrangement of the IgH gene and B lymphocyte differentiation markers. B31-1 cells restore an in vivo repopulation activity to lethally irradiated mice, and the repopulated cells differentiate to HSA+ pre-B cells.Continuous coculture results in two distinct populations, B220(-) c-Kit+ cells and B220(+) c-Kit+ cells; B220(-) c-Kit+ cells are self-renewed and differentiate to B220(+) c-Kit+ cells, while B220(+) c-Kit+ cells produce only B220(+) c-Kit+ cells. Both B220(-) and B220(+) cells similarly express the IgH germ-line transcript (Imu), mRNAs for recombinase (TdT, Rag-1, and Rag-2), and lymphoid-specific transcription factors (Pax-5, EBF, E12/E47, Oct-2, and Ikaros), but the DNA binding activity of Pax-5, EBF, Oct-2, and E2A are low in B220(-) cells and while high in B220(+) cells. These results suggest the existence of at least two active states in the IgH locus before the induction of IgH gene rearrangement during B lymphopoietic development. (+info)
Combined introduction of Bmi-1 and hTERT immortalizes human adipose tissue-derived stromal cells with low risk of...
TY - JOUR. T1 - Combined introduction of Bmi-1 and hTERT immortalizes human adipose tissue-derived stromal cells with low risk of transformation. AU - Tátrai, Péter. AU - Szepesi, Áron. AU - Matula, Zsolt. AU - Szigeti, Anna. AU - Buchan, Gyöngyi. AU - Mádi, András. AU - Uher, Ferenc. AU - Német, Katalin. PY - 2012/5/25. Y1 - 2012/5/25. N2 - Adipose tissue-derived stromal cells (ASCs) are increasingly being studied for their usefulness in regenerative medicine. However, limited life span and donor-dependent variation of primary cells such as ASCs present major hurdles to controlled and reproducible experiments. We therefore aimed to establish immortalized ASC cell lines that provide steady supply of homogeneous cells for in vitro work while retain essential features of primary cells. To this end, combinations of human telomerase reverse transcriptase (hTERT), murine Bmi-1, and SV40 large T antigen (SV40T) were introduced by lentiviral transduction into ASCs. The resulting cell lines ASC ...
Adipose stromal cells differentiation toward smooth muscle cell phenotype diminishes their vasculogenic activity due to...
TY - JOUR. T1 - Adipose stromal cells differentiation toward smooth muscle cell phenotype diminishes their vasculogenic activity due to induction of activin A secretion. AU - Merfeld-Clauss, Stephanie. AU - Lease, Benjamin R.. AU - Lu, Hongyan. AU - March, Keith L.. AU - Traktuev, Dmitry O.. PY - 2017/11. Y1 - 2017/11. N2 - Adipose stromal cells (ASCs) support endothelial cell (EC) vasculogenesis through paracrine and cell-contact communications. In addition, ASCs differentiate towards the smooth muscle cell (SMC) phenotype under different stimuli, which prompted their use as a source of mural cells in fabricating small calibre vessels. How ASCs SMC-lineage commitment affects their subsequent communication with ECs is unknown. The vasculogenic characteristics of human ASCs in progenitor stage and after differentiation towards SMC phenotype were analysed in the present study. Exposure to transforming growth factor β1 (TGFβ1) or activin A has induced expression of SMC markers in ASCs. Analysis ...
AID 44634 - HSF produced by bone marrow-derived stromal cell lines C6.4 on stimulation with the compound at (1000 ng/mL) was...
Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study - pdf...
Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Intracerebral Xenotransplantation of GFP Mouse Bone Marrow Stromal Cells in Intact and Stroke Rat Brain: Graft Survival and...
TY - JOUR. T1 - Intracerebral Xenotransplantation of GFP Mouse Bone Marrow Stromal Cells in Intact and Stroke Rat Brain. T2 - Graft Survival and Immunologic Response. AU - Irons, H.. AU - Lind, J. G.. AU - Wakade, Chandramohan G.. AU - Yu, G.. AU - Hadman, M.. AU - Carroll, James Edwin. AU - Hess, David C. AU - Borlongan, Cesar V.. PY - 2004/1/1. Y1 - 2004/1/1. N2 - The present study characterized survival and immunologic response of bone marrow stromal cells (BMSCs) following transplantation into intact and stroke brains. In the first study, intrastriatal transplantation of BMSC (60,000 in 3 μl) or vehicle was performed in normal adult Sprague-Dawley male rats that subsequently received daily cyclosporin A (CsA, 10 mg/kg, IP in 3 ml) or vehicle (olive oil, similar volume) starting on day of surgery up to 3 days posttransplantation. Animals were euthanized at 3 or 30 days posttransplantation and brains were processed either for green fluorescent protein (GFP) microscopy or flow cytometry ...
Screening differentially expressed proteins from co-cultured hematopoietic cells and bone marrow-derived stromal cells by...
Bone marrow stromal cells protect hematopoietic cells and provide drug resistance by delivering bunch of variable proteins. Thus, alterations of protein expression are typically associated with cell-cell signal transduction and regulation of cellular functions. Co-culture models of bone marrow stromal cells and hematopoietic cells are often used in studies of their crosstalk. Studies of altered protein expression initiated by stromal cell/hematopoietic cell interactions are an important new trend in microenvironmental research. There has been no report to date of global quantitative proteomics analysis of crosstalk between hematopoietic cells and stromal cells. In this study, we analyzed quantitative proteomes in a co-culture system of stromal HS5 cells and hematopoietic KG1a cells, and simultaneously tracked differentially expressed proteins in two types of cells before and after co-culture by stable isotope labeling by amino acids in cell culture (SILAC) method. We have shown that in co-cultured KG1a,
USE OF ADIPOSE-DERIVED PREADIPOCYTES IN BONE DISOREDERS | SBIR.gov
Non-healing bone fractures and periodontal bone loss constitute significant clinical problems with few approved medical options. Bone repair is enhanced by the presence of osteoblasts or osteoblastic precursor cells. Subcutaneous adipose tissue is a plentiful, accessible, and replenishable source of human stromal cells for transplantation. In Phase I of this SBIR, we tested the hypothesis that human adipose tissue-derived stromal cells are capable of osteoblast function. Substantial in vitro data indicates that these stromal cells differentiate into cells biochemically and morphologically similar to osteoblasts. The ability of these cells to form bone in vivo was examined as well. Phase II of this SBIR will extend these in vivo experiments. Specific Aim 1 examines the ability of human adipose tissue-derived stromal cells to form ectopic bone in hydroxyapatite ceramic cubes implanted subcutaneously in immunodeficient mice. Specific Aim 2 explores whether the introduction of a modified bone ...
Epidermal Growth Factor and Transforming Growth Factor-α Stimulate the Proliferation of Mouse Uterine Stromal Cells - OKAYAMA...
Growth factors produced in the uterine endometrium are considered to be involved in the proliferation of the mouse uterine stromal cells induced by estradiol-17beta (E-2) and progesterone (P). The effect of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha), one of EGF-related growth factors, on the proliferation of mouse uterine stromal cells was studied in a serum-free culture. The growth of the uterine stromal cells was measured by MTT assay. EGF was found to increase the number of uterine stromal cells in a dose-dependent manner. The DNA-replicating cells were investigated using the immunocytochemical detection of bromodeoxyuridine (BrdU)-labeled cells. EGF and TGF-alpha increased the percentage of BrdU-Iabeled cells in a dose-dependent manner. Administration of the combination of E-2 (10(-9) M) and P (10(-7) M) for 2 days increased the percentage of BrdU-Iabeled cells 2.3-fold. The stimulatory effect of EGF, TGF-a and the combination of E2 and P on DNA ...
Aminobisphosphonates prevent the inhibitory effects exerted by lymph node stromal cells on anti-tumor Vdelta 2 T lymphocytes in...
In this study, we analyzed the influence of mesenchymal stromal cells derived from lymph nodes of non-Hodgkin lymphomas, on effector functions and differentiation of Vδ2 T lymphocytes. We show that: i) lymph-node mesenchymal stromal cells of non-Hodgkin lymphoma inhibit NKG2D-mediated lymphoid cell killing, but not rituximab-induced antibody-dependent cell-mediated cytotoxicity, exerted by Vδ2 T lymphocytes; ii) pre-treatment of mesenchymal stromal cells with the aminobisphosphonates pamidronate or zoledronate can rescue lymphoma cell killing via NKG2D; iii) this is due to inhibition of transforming growth factor-β and increase in interleukin-15 production by mesenchymal stromal cells; iv) aminobiphosphonate-treated mesenchymal stromal cells drive Vδ2 T lymphocyte differentiation into effector memory T cells, expressing the Thelper1 cytokines tumor necrosis factor-α and interferon-γ. In non-Hodgkin lymphoma lymph-nodes, Vδ2 T cells were mostly naive; upon co-culture with autologous ...
In this study, the role of histamine in interleukin-1 (IL-1) formation in murine bone marrow stromal cells was investigated in vitro. It was found that histamine and 4-methylhistamine increased the number of granulocyte colony-forming units in murine bone marrow cells. A similar effect was elicited by dibutyryl-cAMP and theophylline. When histamine and H2 agonists, such as 4-methylhistamine and dimaprit, were added to the culture medium containing murine bone marrow stromal cells, thymocyte comitogenic activity detected in the medium increased significantly. However, no such effect was observed in the case of 2-methyl-histamine, an H1 agonist. Histamine-induced production of thymocyte comitogenic activity in bone marrow stromal cells was inhibited by some H2 antagonists, such as cimetidine, ranitidine, and famotidine, but not by the H1 antagonist pyrilamine. Histamine was also effective in inducing the colony-promoting activity in murine bone marrow stromal cells. This was also inhibited by H2 ...
Paper: Characterization of Human Bone Marrow Stromal Cells During Multi-passaging (AADR Annual Meeting (March 21-24, 2012))
Objectives: Human bone marrow stromal cells (hBMSCs) are adherent fibroblast-like cells found in the bone marrow. They are a heterogeneous population of cells that includes a subset of osteoprogenitors. BMSCs have been widely used for tissue engineering, especially for bone regeneration. However, for clinical application currently, large quantities of hBMSCs are usually required for transplantation which is typically produced by serial passages of the cells ex vivo. We examined the effects of in vitro expansion on hBMSCs proliferation, multidifferentiation, and gene expression profiles. Methods: hBMSCs were harvested from surgical waste bone specimens from 3 healthy adults with IRB approval. The hBMSCs were cultured in α-MEM with 10% FBS and 1% penicillin-streptomycin. hBMSCs were trypsinized and passaged when they reached 70-80% confluence. Cells from early passage (p2 or 3) were compared with late passage (p7 or 8). MTT assay was used to determine the growth kinetics of hBMSCs. ...
Synthesis of calbindin-D28K during mineralization in human bone marrow stromal cells | Biochemical Journal
1α,25-Dihydroxyvitamin D3 [1,25(OH)2D3] is known to modulate Ca2+ metabolism in several cell types. Vitamin-D-dependent calcium binding proteins such as calbindin-D28K (28 kDa calcium binding proteins) have been shown to be regulated by 1,25(OH)2D3 but the mechanisms controlling calbindin synthesis are still poorly understood in human osteoblast cell culture models. The human bone marrow stromal cells (HBMSC) described in this paper developed a calcified matrix, expressed osteocalcin (OC), osteopontin (OP) and responded to 1,25(OH)2D3. The expression of vitamin D receptor mRNA was demonstrated by reverse transcription-PCR. Calbindin-D28K protein was identified only in cells arising from the sixth subculture, which exhibited a calcified matrix and all of the osteoblastic markers, e.g. OC and OP. It was demonstrated by dot-immunodetection using immunological probes, and by in situ hybridization using labelled cDNA probes. Moreover, vitamin D3 enhanced calbindin-D28K synthesis as well as OC ...
Impaired lymph node stromal cell function during the earliest phases of rheumatoid arthritis | Arthritis Research & Therapy |...
Systemic autoimmunity can be present years before clinical onset of rheumatoid arthritis (RA). Adaptive immunity is initiated in lymphoid tissue where lymph node stromal cells (LNSCs) regulate immune responses through their intimate connection with leucocytes. We postulate that malfunctioning of LNSCs creates a microenvironment in which normal immune responses are not properly controlled, possibly leading to autoimmune disease. In this study we established an experimental model for studying the functional capacities of human LNSCs during RA development. Twenty-four patients with RA, 23 individuals positive for autoantibodies but without clinical disease (RA risk group) and 14 seronegative healthy control subjects underwent ultrasound-guided inguinal lymph node (LN) biopsy. Human LNSCs were isolated and expanded in vitro for functional analyses. In analogous co-cultures consisting of LNSCs and peripheral blood mononuclear cells, αCD3/αCD28-induced T-cell proliferation was measured using
"The Effect of αCT-1 Peptide on Bone Marrow Stromal Cells Following Inj" by Adam Clay Vandergriff
Connexin43 (Cx43) is a component of gap junctions and is involved in intercel- lular signaling following injury to tissues. The carboxyl terminus of Cx43 binds to the PDZ2 domain of ZO-1 in order to form gap junction plaques and connect to the cytoskeleton. A biomimetic peptide known as αCT-1, replicating the last 9 amino acids found in the carboxyl terminus of CX43, has been shown to improve wound healing by preferentially binding to the PDZ2 domain of ZO-1. A possible mecha- nism for its action is through the Epithelial-Mesenchymal Transformation (EMT). Scratch assays were performed on rat bone marrow stromal cells treated with the peptide and were then analyzed using qPCR, western blotting, confocal microscopy, and live cell imaging. The gene expression analysis showed up-regulation of F11r and Krt19 and down-regulation of Mmp3. Protein expression analysis indicated an increase in Krt19 and the complete absence of Snai2 in the αCT-1 treated samples. Confocal microscopy suggested increased actin
Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis - US Stemology
Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis Cognizant Communication Corporation Source Cognizant Communication Corporation DOI: 10.3727/096368915X686760 Jaroslav Michalek1*, Rene Moster2, Ladislav Lukac3, Kenneth Proefrock4, Miron Petrasovic5, Jakub Rybar5, Martina Capkova6, Ales Chaloupka7, Adas Darinskas8, Jaroslav Michalek, sr.9, Jan Kristek10, Jan Travnik11, Petr Jabandziev12, Marek…
Locally delivered growth factor enhances the angiogenic efficacy of adipose-derived stromal cells transplanted to ischemic...
TY - JOUR. T1 - Locally delivered growth factor enhances the angiogenic efficacy of adipose-derived stromal cells transplanted to ischemic limbs. AU - Bhang, Suk Ho. AU - Cho, Seung Woo. AU - Lim, Jae Min. AU - Kang, Jin Muk. AU - Lee, Tae Jin. AU - Yang, Hee Seok. AU - Song, Young Soo. AU - Park, Moon Hyang. AU - Kim, Hyo Soo. AU - Yoo, Kyung Jong. AU - Jang, Yangsoo. AU - Langer, Robert. AU - Anderson, Daniel G.. AU - Kim, Byung Soo. PY - 2009/8/1. Y1 - 2009/8/1. N2 - Ischemia is a potentially fatal medical event that is associated with as many as 30% of all deaths. Stem cell therapy offers significant therapeutic promise, but poor survival following transplantation to ischemic tissue limits its efficacy. Here we demonstrate that nanosphere-mediated growth factor delivery can enhance the survival of transplanted human adipose-derived stromal cells (hADSCs) and secretion of human angiogenic growth factors per cell, and substantially improve therapeutic efficacy of hADSCs. In vitro, in hypoxic ...
Stromal Cells in Immunity and Disease 20Q5 - Summary
Non-hematopoietic stromal cells play important roles in many tissues, constructing tissue microenvironments, contributing to tissue repair, defense and immune responses. Within lymphoid organs, stromal cells organize and interact with leukocytes in an immunologically important manner. In addition to organizing T and B cell segregation and expressing lymphocyte survival factors, stromal cells support the migration and interactions between antigen presenting cells and naïve T and B cells during the initiation of immune responses and influence the outcome between tolerance and immunity. Stromal cells also play instrumental roles in coordinating immune responses in non-lymphoid tissues, in inflammatory and autoimmune diseases, and in chronic infection. For instance, stromal cell dysregulation has been seen in HIV infection and in cancer patients. Furthermore, stromal cells are being harnessed for therapeutic applications in several diseases, an area that holds great promise for improving human ...
Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation | Stem...
Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions. In this review, we try to address several important aspects of this field, outlining the biology, technology, translation, and challenges related to SVF- and ADSC-based therapies. Starting from the basics of SVF and ADSC isolation, we touch upon recently developed technologies, addressing elements of novel methods and devices under development for point-of-care isolation of SVF. Characterisation of SVF cells and ADSCs is also an evolving area and we look into unusual expression of CD34 antigen as an
TNFα and IL1β Stimulate Dif-ferential Gene Expression in Endometrial Stromal Cells
The purpose of this study was to test the hypothesis that specific macrophage-secreted cytokines cause gene expression changes in endometrial stromal cells that reproduce the effects of macro-phages in the development of endometriosis. Telomerase-immortalized human endometrial stromal cells (T-HESC) were treated with tumor necrosis factor α (TNFα, 5 ng/ml) and interleukin 1β (IL1β, 1 ng/ml). Differential expression of 249 genes was identified by DNA microarray. Ontologies such as peptidases, cell adhesion, cell death/cell cycle, growth factors, cytoskeletal organization, defense/immune system, signal transduction, and transcriptional regulation which are related to the development of endometriosis were represented by these genes. The up-regulation of interleukin 8 (IL8), interleukin 6 (IL6), IL1β and matrix metallopro-teinase 3 (MMP3) in response to TNFα ± ILIβ in T-HESC cells was confirmed by real time RT-PCR. TNFα ± ILIβ did not affect the migration or invasion of T-HESC cells. This study
CD90<sup>+</sup> stromal cells are the major source of IL-6, which supports cancer stem-like cells and inflammation in...
TY - JOUR. T1 - CD90+ stromal cells are the major source of IL-6, which supports cancer stem-like cells and inflammation in colorectal cancer. AU - Huynh, Phuong T.. AU - Beswick, Ellen J.. AU - Coronado, Yun A.. AU - Johnson, Paul. AU - OConnell, Malaney R.. AU - Watts, Tammara. AU - Singh, Pomila. AU - Qiu, Suimin. AU - Morris, Katherine. AU - Powell, Don W.. AU - Pinchuk, Irina V.. PY - 2016/4/15. Y1 - 2016/4/15. N2 - IL-6 is a pleiotropic cytokine increased in CRC and known to directly promote tumor growth. Colonic myofibroblasts/fibroblasts (CMFs or stromal cells) are CD90+ innate immune cells representing up to 30% of normal colonic mucosal lamina propria cells. They are expanded in CRC tumor stroma, where they also known as a cancer associated fibroblasts (CAFs). Cells of mesenchymal origin, such as normal myofibroblasts/fibroblasts, are known to secrete IL-6; however, their contribution to the increase in IL-6 in CRC and to tumor-promoting inflammation is not well defined. Using in ...
Brain from bone: Efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with Noggin or a...
TY - JOUR. T1 - Brain from bone. T2 - Efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent. AU - Kohyama, Jun. AU - Abe, Hitoshi. AU - Shimazaki, Takuya. AU - Koizumi, Amane. AU - Nakashima, Kinichi. AU - Gojo, Satoshi. AU - Taga, Tetsuya. AU - Okano, Hideyuki. AU - Hata, Jun Ichi. AU - Umezawa, Akihiro. PY - 2001/10. Y1 - 2001/10. N2 - Bone marrow stromal cells are able to differentiate into adipogenic, chondrogenic, myogenic, osteogenic, and cardiomyogenic lineages, all of which are limited to a mesoderm-derived origin. In this study, we showed that neurons, which are of an ectoderm-origin, could be generated from marrow-derived stromal cells by specific inducers, fibronectin/ornithine coating, and neurosphere formation. The neurons generated from marrow stroma formed neurites, expressed neuron-specific markers and genes, and started to respond to depolarizing stimuli as functional mature neurons. Among stromal cells, ...
Stromal cell - Wikipedia
Stromal cells are connective tissue cells of any organ, for example in the uterine mucosa (endometrium), prostate, bone marrow, lymph node and the ovary. They are cells that support the function of the parenchymal cells of that organ. Fibroblasts and pericytes are among the most common types of stromal cells. The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. In addition, by regulating local cytokine networks (e.g. M-CSF, LIF), bone marrow stromal cells have been described to be involved in human haematopoiesis and inflammatory processes. Stromal cells (in the dermis layer) adjacent to the epidermis (the very top layer of the skin) release growth factors that promote cell division. This keeps the epidermis regenerating from the bottom while the top layer of cells on the epidermis are constantly being "sloughed" off the body. Certain types of skin cancers (basal cell carcinomas) cannot spread throughout the body because the cancer ...
A novel protein Depp, which is induced by progesterone in human endometrial stromal cells activates Elk-1 transcription factor
Decidualization of the endometrial stromal cells (ESC), considered to be stimulated by progesterone and/or cAMP, is crucial for embryo implantation and placentation. In this study, we isolated a novel clone encoding decidual protein induced by progesterone (Depp) from a human ESC cDNA library enrich …
Aging | Molecular basis of senescence transmitting in the population of human endometrial stromal cells - Figure
Hormone-regulated proliferation and differentiation of endometrial stromal cells (ESCs) determine overall endometrial plasticity and receptivity to embryos. Previously we revealed that ESCs may undergo premature senescence, accompanied by proliferation loss and various intracellular alterations. Here we focused on whether and how senescence may be transmitted within the ESCs population. We revealed that senescent ESCs may induce paracrine senescence in young counterparts via cell contacts, secreted factors and extracellular vesicles. According to secretome-wide profiling we identified plasminogen activator inhibitor -1 (PAI-1) to be the most prominent protein secreted by senescent ESCs (data are available via ProteomeXchange with identifier PXD015742). By applying CRISPR/Cas9 techniques we disclosed that PAI-1 secreted by senescent ESCs may serve as the master-regulator of paracrine senescence progression within the ESCs population. Unraveled molecular basis of senescence transduction in the ESCs
In vivo gene manipulation reveals the impact of stress-responsive MAPK pathways on tumor progression - Semantic Scholar
It has been widely accepted that tumor cells and normal stromal cells in the host environment coordinately modulate tumor progression. Mitogen-activated protein kinase pathways are the representative stress-responsive cascades that exert proper cellular responses to divergent environmental stimuli. Genetically engineered mouse models and chemically induced tumorigenesis models have revealed that components of the MAPK pathway not only regulate the behavior of tumor cells themselves but also that of surrounding normal stromal cells in the host environment during cancer pathogenesis. The individual functions of MAPK pathway components in tumor initiation and progression vary depending on the stimuli and the stromal cell types involved in tumor progression, in addition to the molecular isoforms of the components and the origins of the tumor. Recent studies have indicated that MAPK pathway components synergize with environmental factors (e.g. tobacco smoke and diet) to affect tumor initiation and
In vitro osteogenic potential of human bone marrow stromal cel...
Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture. | JCB
Physical interaction between human lymphomas and murine bone marrow derived stromal cells were studied. Nalm-6 pre-B cells adhered to BMS2 stromal cells and subsequently migrated beneath them, while Ramos Burkitt lymphoma cells, adhered but did not migrate. Four mAbs were established against Nalm-6 cells, which were able to block initial adhesion of Nalm-6 cells. Two of them were directed against the alpha 4 chain of VLA-4, and other two recognized the beta 1 chain of VLA integrins. Therefore, the initial adhesion of Ramos and Nalm-6 cells to BMS2 was largely mediated by the VLA-4 integrin expressed on lymphocytes. The corresponding ligand on stromal cells appears to be VCAM-1, because antibodies against murine VCAM-1 blocked the adhesion. However, antibodies against the alpha chain of VLA-4 were not capable of blocking subsequent migration beneath stromal cells. In contrast, antibodies against the beta chain of VLA integrins blocked the migration beneath stromal cells as well as the initial ...
Profiling of circadian genes expressed in the uterus endometrial stromal cells of pregnant rats as revealed by DNA microarray...
TY - JOUR. T1 - Profiling of circadian genes expressed in the uterus endometrial stromal cells of pregnant rats as revealed by DNA microarray coupled with RNA interference. AU - Tasaki, Hirotaka. AU - Zhao, Lijia. AU - Isayama, Keishiro. AU - Chen, Huatao. AU - Yamauchi, Nobuhiko. AU - Shigeyoshi, Yasufumi. AU - Hashimoto, Seiichi. AU - Hattori, Masa aki. PY - 2013. Y1 - 2013. N2 - The peripheral circadian oscillator plays an essential role in synchronizing local physiology to operate in a circadian manner via regulation of the expression of clock-controlled genes. The present study aimed to evaluate the circadian rhythms of clock genes and clock-controlled genes expressed in the rat uterus endometrial stromal cells (UESCs) during the stage of implantation by a DNA microarray. Of 12,252 genes showing significantly expression, 7,235 genes displayed significant alterations. As revealed by the biological pathway analysis using the database for annotation, visualization, and integrated discovery ...
TGF-β signaling in stromal cells acts upstream of FGF-10 to regulate epithelial stem cell growth in the adult lung<...
TY - JOUR. T1 - TGF-β signaling in stromal cells acts upstream of FGF-10 to regulate epithelial stem cell growth in the adult lung. AU - McQualter, Jonathan L.. AU - McCarty, Rosa C.. AU - Van der Velden, Joanne. AU - ODonoghue, Robert J.J.. AU - Labat, Marie-Liesse. AU - Bozinovski, Steven. AU - Bertoncello, Ivan. PY - 2013/11/1. Y1 - 2013/11/1. N2 - Tissue resident mesenchymal stromal cells (MSCs) contribute to tissue regeneration through various mechanisms, including the secretion of trophic factors that act directly on epithelial stem cells to promote epithelialization. However, MSCs in tissues constitute a heterogeneous population of stromal cells and different subtypes may have different functions. In this study we show that CD166neg and CD166pos lung stromal cells have different proliferative and differentiative potential. CD166neg lung stromal cells exhibit high proliferative potential with the capacity to differentiate along the lipofibroblastic and myofibroblastic lineages, whereas ...
OP0258 IL-22 Regulates Lymphoid Stromal Cell Expansion, Development of Germinal Centers and Humoral Response in Mucosal Ectopic...
Results WT mice stromal cells display early acquisition of lymphoid features, up-regulating gp38 already at 3hours pc. Peak of this activation was observed at day5 with significant increase in the percentage of gp38+ lymphoid like stromal cells (LLSc), this increase is maintained between day8-15 pc. However, full acquisition of lymphoid phenotype, indicated by high levels of lymphoid CKs/cytokine expression was observed by day8-15 pc. This correlates with full maturation of the lymphoid aggregates in terms of T/B cell segregation and FDC formation. Of interest, RAG and LTbR KO mice showed normal degree of stromal cell activation in the early phases (2-5days p.c.) but a dramatic decrease in the percentage of LLSc by day15. However, these KOs exhibited a defect in full lymphoid conversion of stromal cells shown by significant decrease in CKs/cytokine expression and aggregates disorganization, suggesting that the full maturation of stromal cells require lymphocyte-derived signals such as ...
The interferon-inducible protein p202 promotes osteogenesis in mouse bone marrow stromal cells | Bioscience Reports | Portland...
Finally, the mechanism by which p202 activates BMSC osteogenesis was determined. Runx2 is a critical transcription factor in osteogenesis. Previous study has detected the association of p204 with Runx2 . However, in the present study, no interaction of p202 with Runx2 was seen (Figure 5C). p202 may lack an interacting structure with Runx2 protein. Id proteins are important suppressors in the differentiation of many cell types [13,20,21]. We found that Id proteins not only bound to Runx2, but also associated with p202 in the course of BMSC osteogenesis, and Id2 was a major associated family member (Figure 5A,B). It is possible that p202 disturbs the formation of Runx2/Ids complex and frees Runx2 to induce the differentiation process. Subsequent investigation demonstrated that this is the case. SiRNA-p202 dramatically lowered the p202-bound Id2, while enhanced the Runx2-associated Id2 content significantly. However, p202 overexpression increased the p202-bound Id2, but decreased the ...
Endothelial cells influence the osteogenic potential of bone marrow stromal cells
Background: Improved understanding of the interactions between bone cells and endothelial cells involved in osteogenesis should aid the development of new strategies for bone tissue engineering. The aim of the present study was to determine whether direct communication between bone marrow stromal cells (MSC) and human umbilical vein endothelial cells (EC) could influence the osteogenic potential of MSC in osteogenic factor-free medium. Methods: After adding EC to MSC in a direct-contact system, cell viability and morphology were investigated with the WST assay and immnostaining. The effects on osteogenic differentiation of adding EC to MSC was systematically tested by the using Superarray assay and results were confirmed with real-time PCR. Results: Five days after the addition of EC to MSC in a ratio of 1:5 (EC/MSC) significant increases in cell proliferation and cellular bridges between the two cell types were detected, as well as increased mRNA expression of alkaline phosphatase (ALP). This ...
"Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells a" by David E Maridas, Elizabeth Rendina-Ruedy et al.
Bone marrow stromal cells (BMSCs) constitute a cell population routinely used as a representation of mesenchymal stem cells in vitro. They reside within the bone marrow cavity alongside hematopoietic stem cells (HSCs), which can give rise to red blood cells, immune progenitors, and osteoclasts. Thus, extractions of cell populations from the bone marrow results in a very heterogeneous mix of various cell populations, which can present challenges in experimental design and confound data interpretation. Several isolation and culture techniques have been developed in laboratories in order to obtain more or less homogeneous populations of BMSCs and HSCs invitro. Here, we present two methods for isolation of BMSCs and HSCs from mouse long bones: one method that yields a mixed population of BMSCs and HSCs and one method that attempts to separate the two cell populations based on adherence. Both methods provide cells suitable for osteogenic and adipogenic differentiation experiments as well as functional assays
Human Adipose Stromal/Stem Cells from Obese Donors Show Reduced Efficacy in Halting Disease Progression in the Experimental...
Multiple sclerosis is an autoimmune disease that affects the white matter of the central nervous system and involves inflammation and demyelination. The recent advances in our understanding of adipose-derived stromal/stem cells (ASCs) and the utilization of these cells in clinical settings to treat …
Gateway-compatible transposon vector to genetically modify human embryonic kidney and adipose-derived stromal cells. - Petrakis...
NCHU Institutional Repository CRIS: Transplantation of bone marrow stromal cells for peripheral nerve repair
Cell transplantation using bone marrow stromal cells (BMSCs) to alleviate neurological deficits has recently become the focus of research in regenerative medicine. Evidence suggests that secretion of various growth-promoting substances likely plays an important role in functional recovery against neurological diseases. In an attempt to identify a possible mechanism underlying the regenerative potential of BMSCs, this study investigated the production and possible contribution of neurotrophic factors by transected sciatic nerve defect in a rat model with a 15 mm gap. Cultured BMSCs became morphologically homogeneous with fibroblast-like shape after ex vivo expansion. We provided several pieces of evidence for the beneficial effects of implanted fibroblast-like BMSCs on sciatic nerve regeneration. When compared to silicone tube control animals, this treatment led to (i) improved walking behavior as measured by footprint analysis, (ii) reduced loss of gastrocnemius muscle weight and EMG magnitude, ...
Interleukin 7 independent development of human B cells | PNAS
Mammalian hematopoietic stem cell (HSC) commitment and differentiation into lymphoid lineage cells proceed through a series of developmentally restricted progenitor compartments. A complete understanding of this process, and how it differs from HSC commitment and differentiation into cells of the myeloid/erythroid lineages, requires the development of model systems that support HSC commitment to the lymphoid lineages. We now describe a human bone marrow stromal cell culture that preferentially supports commitment and differentiation of human HSC to CD19+ B-lineage cells. Fluorescence activated cell sorterpurified CD34++/lineage-cells were isolated from fetal bone marrow and cultured on human fetal bone marrow stromal cells in serum-free conditions containing no exogenous cytokines. Over a period of 3 weeks, CD34++/lineage- cells underwent commitment, differentiation, and expansion into the B lineage. Progressive changes included: loss of CD34, acquisition of and graded increases in the level of ...
Surface epithelial-stromal tumor - wikidoc
Surface epithelial-stromal tumors are a class of ovarian neoplasms that may be benign or malignant. Neoplasms in this group are thought to be derived from the ovarian surface epithelium (modified peritoneum) or from ectopic endometrial or Fallopian tube (tubal) tissue. This group of tumors accounts for 90% to 95% of all cases of ovarian cancer. The pathogenesis of surface epithelial-stromal tumor is characterized by the overgrowth of the ovarian surface epithelium. Common risk factors in the development of surface epithelial-stromal tumor, include: nulliparity, early menopause, gonadal dysgenesis, family history (e.g. BRCA1/BRCA2 mutations), smoking, previous history of breast, and endometrial or colon cancer (Lynch II). The prevalence of surface epithelial-stromal tumor is approximately 3 per 100,000 individuals worldwide. Surface epithelial-stromal tumor is more commonly observed among postmenopausal women. Early clinical features of surface epithelial-stromal tumor include pelvic ...
PSRC - RESTORATION OF LONG NERVE DEFECTS WITH EPINEURAL SHEATH CONDUIT SUPPORTED WITH BONE MARROW STROMAL CELLS. A PRELIMINARY...
Cleveland Clinic Background: Nerve gaps that need conduit or allograft material tend to involve regional loss of overlying soft tissues and muscles spanning a gap of over 20 cm. Currently used autograft technique requires immunosuppression and demonstrated poor motor recovery.. Main goals: We developed epineural sheath conduit supported with bone marrow stromal cells (BMSCs) to restore 6 cm nerve defects. Epineural sheath is immunologically neutral and contains laminin, enhancing neuronal growth. Addition of BMSCs will contribute to structural support and secretion of growth factors for nerve regeneration.. Methods: Sheep model was used since sheep peripheral nerves are histologically and morphometrically similar to human nerves. Epineural sheath tube was created from the median nerve by the pull out technique, removing all fascicles. BMSCs were obtained from donor animal, purified by the buffy coat technique and cultured for 14 days. Next, cells were fluorescently labeled and injected into the ...
IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction...
The activated tumor stroma participates in many processes that control tumorigenesis, including tumor cell growth, invasion and metastasis. Cancer-associated fibroblasts (CAFs) represent the major cellular component of the stroma and are the main source for connective tissue components of the extracellular matrix and various classes of proteolytic enzymes. The signaling pathways involved in the interactions between tumor and stromal cells and the molecular characteristics that distinguish normal resting fibroblasts from cancer-associated or -activated fibroblasts remain poorly defined. Recent studies emphasized the prognostic and therapeutic significance of CAF-related molecular signatures and a number of those genes have been shown to serve as putative therapeutic targets. We have used immuno-laser capture microdissection and whole-genome Affymetrix GeneChip analysis to obtain transcriptional signatures from the activated tumor stroma of colon carcinomas that were compared with normal resting
Primary Human Prostate Stromal Cells | Creative Bioarray
Frontiers | Alteration of the antitumor immune response by cancer-associated fibroblasts | Immunology
Among cells present in the tumor microenvironment, activated fibroblasts termed cancer-associated fibroblasts (CAFs), play a critical role in the complex process of tumor-stroma interaction. CAFs, one of the prominent stromal cell populations in most types of human carcinomas, have been involved in tumor growth, angiogenesis, cancer stemness, extracellular matrix remodeling, tissue invasion, metastasis and even chemoresistance. During the past decade, these activated tumor-associated fibroblasts have also been involved in the modulation of the anti-tumor immune response on various levels. In this review, we describe our current understanding of how CAFs accomplish this task as well as their potential therapeutic implications.
Epigenetically Aberrant Stroma in MDS Propagates Disease via Wnt/β-Catenin Activation. - Radcliffe Department of Medicine
The bone marrow microenvironment influences malignant hematopoiesis, but how it promotes leukemogenesis has not been elucidated. In addition, the role of the bone marrow stroma in regulating clinical responses to DNA methyltransferase inhibitors (DNMTi) is also poorly understood. In this study, we conducted a DNA methylome analysis of bone marrow-derived stromal cells from myelodysplastic syndrome (MDS) patients and observed widespread aberrant cytosine hypermethylation occurring preferentially outside CpG islands. Stroma derived from 5-azacytidine-treated patients lacked aberrant methylation and DNMTi treatment of primary MDS stroma enhanced its ability to support erythroid differentiation. An integrative expression analysis revealed that the WNT pathway antagonist FRZB was aberrantly hypermethylated and underexpressed in MDS stroma. This result was confirmed in an independent set of sorted, primary MDS-derived mesenchymal cells. We documented a WNT/β-catenin activation signature in CD34+ cells from
The bone marrow microenvironment influences malignant hematopoiesis but how it promotes leukemogenesis has not been elucidated. Additionally, the role of the bone marrow stroma in regulating clinical responses to DNA methyltransferase inhibitors (DNMTi) is also poorly understood. In this study, we conducted a DNA methylome analysis of bone marrow-derived stromal cells from myelodysplastic syndrome (MDS) patients and observed widespread aberrant cytosine hypermethylation occurring preferentially outside CpG islands. Stroma derived from 5-azacytidine-treated patients lacked aberrant methylation and DNMTi treatment of primary MDS stroma enhanced its ability to support erythroid differentiation. An integrative expression analysis revealed that the WNT pathway antagonist FRZB was aberrantly hypermethylated and underexpressed in MDS stroma. This result was confirmed in an independent set of sorted, primary MDS-derived mesenchymal cells. We documented a WNT/ß-catenin activation signature in CD34+ ...
In this study, we characterized signaling cascades activated on direct contact of leukemic cells with bone marrow-derived stromal cells. Our findings indicate stroma-induced activation of ILK with phosphorylation of Akt and GSK3β. Because p-GSK3βSer9 is a known cellular target of ILK ( 7), these results are consistent with evidence of ILK activation induced by growth factors or engagement of the integrins by stroma. We further showed that the nuclear accumulation of β-catenin, a downstream GSK3β target, was induced in NB4 cells by coculture with MSCs and that this accumulation was abrogated when the PI3K/ILK pathway was inhibited. In contrast, the GSK3 inhibitor BIO, which prevented the serum withdrawal-induced apoptosis of NB4 cells, stimulated the translocation of β-catenin to the nucleus in cells growing without stromal support. Altogether, these findings provide evidence that stroma-induced ILK activation results in GSK3 inhibition and up-regulation of nuclear β-catenin consistent with ...
SILAC Analysis of Two Bone Marrow Stromal Cell Lines
Hormone released by bone marrow cells may hasten recovery from br...( Tampa FL (Feb. 13 2004) -- Bone marro...)
Health,Tampa FL (Feb. 13 2004) -- Bone marrow stromal cells release a blood...The laboratory study was published in the January 2004 issue of the jo... Were suggesting that transplanted bone marrow stromal cells may hast...Researchers at USF and other institutions have demonstrated that some ...However no one has proven that this recovery results from converted b...,Hormone,released,by,bone,marrow,cells,may,hasten,recovery,from,brain,injury,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Stromal Cells May Be Involved in Development of Breast Carcinoma | Cancer Network | The Oncology Journal
High expression of B7-H3 on stromal cells defines tumor and st...
Cyclooxygenase-2 inhibitor celecoxib augments chemotherapeutic drug-induced apoptosis by enhancing activation of caspase-3 and ...
TY - JOUR. T1 - Cyclooxygenase-2 inhibitor celecoxib augments chemotherapeutic drug-induced apoptosis by enhancing activation of caspase-3 and -9 in prostate cancer cells. AU - Dandekar, Devendra S.. AU - Lopez, Monica. AU - Carey, Robert I.. AU - Lokeshwar, Bal L.. PY - 2005/6/20. Y1 - 2005/6/20. N2 - Many tumors constitutively express high levels of the inducible form of proinflammatory enzyme, cyclooxygenase-2 (COX-2). Increased COX-2 expression is associated with tumor cell resistance to many cytotoxic chemotherapy drugs. Furthermore, increased resistance to cytotoxic antitumor drugs is also known to be dependent on associated stromal cells in many tumors. We investigated whether prostate tumor-associated stromal cells, marrow-derived osteoblasts, affect cytotoxicity of 2 antitumor drugs, COL-3 and docetaxel (TXTR), and whether it is dependent on COX-2 activity. We further examined whether inhibiting the activity of COX-2 negate the stroma-induced decrease in drug sensitivity in tumor cells, ...
Osteonecrosis of the jaw
Owen M; Friedenstein AJ (1988). "Stromal stem cells: marrow- derived osteogenic precursors". Ciba Found Symp. 136: 42-60. PMID ... The rapidity with which premature cell death can occur depends on the cell type and the degree and duration of the anoxia. ... In healthy bone these cells are constantly replaced by differentiation of bone marrow mesenchymal stem cells (MSC). However, in ... bone cell damage and eventual cell death (apoptosis). Of significance is the fact that the average concentration of cadmium in ...
Stromal cell secretion of hepatocyte growth factor (HGF). In a phase I clinical study, vemurafenib (then known as PLX4032) was ... Activates the ERK Pathway and Enhances Cell Migration and Proliferation of BRAF(WT) Melanoma Cells". Pigment Cell Melanoma Res ... Vemurafenib causes programmed cell death in melanoma cell lines. Vemurafenib interrupts the B-Raf/MEK step on the B-Raf/MEK/ERK ... Cancer cells begin to overexpress cell surface protein PDGFRB, creating an alternative survival pathway. A second oncogene ...
... is all widely expressed on stromal cells. The N-terminal fragment of GPR126 contains C1r-C1s, Uegf and Bmp1 (CUB), and ... Mogha A, Benesh AE, Patra C, Engel FB, Schöneberg T, Liebscher I, Monk KR (Nov 2013). "Gpr126 functions in Schwann cells to ... Forced GPR126 expression in COS-7 cells enhances cAMP levels by coupling to heterotrimeric Gαs/i proteins. GPR126 has been ... Upon lipopolysaccharide (LPS) or thrombin stimulation, expression of GPR126 is induced by MAP kinases in endothelial cells. ...
J Cell Biol. 133 (2): 257-68. doi:10.1083/jcb.133.2.257. PMC 2120788 . PMID 8609160. "Entrez Gene: SDF4 stromal cell derived ... binding proteins localised to the secretory pathway of mammalian cells". FEBS Lett. 466 (1): 11-8. doi:10.1016/S0014-5793(99) ... Cell. 18 (7): 2473-80. doi:10.1091/mbc.E06-10-0950. PMC 1924827 . PMID 17442889. ...
"Bone marrow stromal stem cells: nature, biology, and potential applications". Stem Cells. 19 (3): 180-92. doi:10.1634/stemcells ... The cell density of full-thickness, human, adult, femoral condyle cartilage is maintained at 14.5 (±3.0) × 103 cells/ mm2 from ... Chondrocytes (from Greek χόνδρος, chondros = cartilage + κύτος, kytos = cell) are the only cells found in healthy cartilage. ... List of human cell types derived from the germ layers. References. *^ Lee, T. J.; Jang, J.; Kang, S.; Jin, M.; Shin, H.; ...
... epithelial and stromal cells in vaginal adenosis show characteristic fusion through the basal lamina or with stromal ... Roberts, Daniel K.; Walker, Nola J.; Parmley, Tim H.; Horbelt, Douglas V. (1988). "Interaction of epithelial and stromal cells ... Its mucinous cells resemble the normal cervical lining, while its tuboendometrial cells resemble the lining of normal fallopian ... Association with clear cell adenocarcinoma in diethylstilbestrol-exposed offspring". Cancer. 54 (5): 869-875. doi:10.1002/1097- ...
2001). "Human thymic stromal lymphopoietin preferentially stimulates myeloid cells". J. Immunol. 167 (1): 336-43. doi:10.4049/ ... 1996). "Interleukin-7 signaling in human B cell precursor acute lymphoblastic leukemia cells and murine BAF3 cells involves ... Banham AH (2007). "Cell-surface IL-7 receptor expression facilitates the purification of FOXP3(+) regulatory T cells". Trends ... Akashi K, Kondo M, Weissman IL (1999). "Role of interleukin-7 in T-cell development from hematopoietic stem cells". Immunol. ...
Stromal tissue falls into the functional class that contributes to the body's support and movement. The cells which make up ... immune system blood cells causing inflammatory response. Fixed cells - cells that are permanent inhabitants of the tissue. ... Stroma is made of various types of stromal cells. Examples of stroma include: stroma of iris stroma of cornea stroma of ovary ... Stromal tissue is primarily made of extracellular matrix containing connective tissue cells. Extracellular matrix is primarily ...
Mir-193 microRNA precursor family
Cismasiu, V. B.; Radu, E.; Popescu, L. M. (2011). "MiR-193 expression differentiates telocytes from other stromal cells". ... Stem Cells and Development. 21 (13): 2508-2519. doi:10.1089/scd.2011.0695. PMC 3424971 . PMID 22384930. ... "MicroRNA-193 Pro-Proliferation Effects for Bone Mesenchymal Stem Cells After Low-Level Laser Irradiation Treatment Through ...
2001). "Human thymic stromal lymphopoietin preferentially stimulates myeloid cells". J. Immunol. 167 (1): 336-43. doi:10.4049/ ... 2007). "Cutting edge: direct action of thymic stromal lymphopoietin on activated human CD4+ T cells". J. Immunol. 178 (11): ... It forms a functional complex with TSLP and IL7R, capable of stimulating cell proliferation through activation of STAT3, STAT5 ... 2002). "Cloning of rat thymic stromal lymphopoietin receptor (TSLPR) and characterization of genomic structure of murine Tslpr ...
Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that ... a surface molecule of bone marrow stromal cell lines that facilitates pre-B-cell growth". Proc Natl Acad Sci U S A. 91 (12): ... "Entrez Gene: BST1 bone marrow stromal cell antigen 1". Human BST1 genome location and BST1 gene details page in the UCSC Genome ... 1996). "Elevated levels of the soluble form of bone marrow stromal cell antigen 1 in the sera of patients with severe ...
Voltage-dependent anion channel
"The Cell Surface Proteome of Human Mesenchymal Stromal Cells". PLoS ONE. 6 (5): e20399. doi:10.1371/journal.pone.0020399. PMC ... and role in mitochondrial function in cell life and death". Cell Biochem. Biophys. 39 (3): 279-92. doi:10.1385/CBB:39:3:279. ... "Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells". J. Cell Biol. 152 (2): 237 ... There is debate as to whether or not this channel is expressed in the cell surface membrane. This major protein of the outer ...
West-Mays JA, Dwivedi DJ (2006). "The keratocyte: corneal stromal cell with variable repair phenotypes". Int. J. Biochem. Cell ... "Interleukin-1alpha downregulates extracellular-superoxide dismutase in human corneal keratoconus stromal cells". Mol. Vis. 13: ... Cell Dev. Biol. 19 (2): 100-12. doi:10.1016/j.semcdb.2007.10.004. PMID 18077195. Lassen N, Pappa A, Black WJ, Jester JV, Day BJ ... Cell Dev. Biol. 19 (2): 82-93. doi:10.1016/j.semcdb.2007.09.015. PMC 2275913 . PMID 17997336. Lassen N, Black WJ, Estey T, ...
"Candida albicans can stimulate stromal cells resulting in enhanced granulopoiesis". Stem Cells Dev. 13 (1): 39-50. doi:10.1089/ ... Pluripotential hemopoietic stem cell Myeloblast Promyelocyte Eosino/neutro/basophilic myelocyte Metamyelocyte Band cell (Stab ... cell) Granulocytes (Eosino/neutro/basophil) It can be stimulated by Candida albicans. Basu S, Zhang HH, Quilici C, Dunn AR ( ...
Gonadal tissue neoplasm
... s are composed of endothelial cells, pericytes and stromal cells. In VHL syndrome the von Hippel-Lindau protein ... Hemangioblastomas are most commonly composed of stromal cells in small blood vessels and usually occur in the cerebellum, brain ... In these dysfunctional cells pVHL cannot degrade HIF-1α, causing it to accumulate. HIF-1α causes the production of vascular ... They may be associated with other diseases such as polycythemia (increased blood cell count), pancreatic cysts and Von Hippel- ...
Forkhead box d1
BF2 and stromal cell have essential function during kidney Morphogenesis. Placental growth factor (PIGF) is a direct and ... Delayed expression of FOX D1 causes decreased glioma cell growth and reduce cell migration. There is a high probability that ... FOX D1 is expressed by two kidney derived cell line COS 7 and 293 cells. It also has high degree of sequence similarity with ... First, PIGF initiates stromal signal that regulate epithelial differentiation which functions as a growth factor in reactive ...
"Cas-L was overexpressed in imatinib-resistant gastrointestinal stromal tumor cells". Cancer Biol. Ther. 8 (8): 683-8. doi: ... Tikhmyanova N, Tulin AV, Roegiers F, Golemis EA (2010). "Dcas supports cell polarization and cell-cell adhesion complexes in ... points of dialog between the cell cycle and cell attachment signaling networks". Cell Cycle. 5 (4): 384-91. doi:10.4161/cc.5.4. ... "Rac activation and inactivation control plasticity of tumor cell movement". Cell. 135 (3): 510-23. doi:10.1016/j.cell.2008.09. ...
Sp7 transcription factor
Tu Q, Valverde P, Chen J (2006). "Osterix enhances proliferation and osteogenic potential of bone marrow stromal cells". ... In mesenchymal precursor cells expressing Runx-2, Sp7 expression will induce these cells to differentiate into osteoblasts, and ... "Telomerase accelerates osteogenesis of bone marrow stromal stem cells by upregulation of CBFA1, osterix, and osteocalcin". ... Kaback LA, Soung do Y, Naik A, Smith N, Schwarz EM, O'Keefe RJ, Drissi H (2008). "Osterix/Sp7 regulates mesenchymal stem cell ...
For example, it regulates endometrial stromal cell proliferation, survival, and differentiation. These processes are all ... Regardless of sex, though, WNT4 is needed for cell proliferation. In mouse gonads, it has been detected only eleven days after ... If deficient in XY mice, there is a delay in Sertoli cell differentiation. Moreover, there is delay in sex cord formation. ... For instance, TAFIIs 105 is now encoded, a subunit of the TATA binding protein for RNA polymerase in ovarian follicle cells. ...
Plant matrix metalloproteinase
Its RNA was taken from stromal cells in human breast carcinomas. Based on the PUMP sequence and functionality of carcinomas in ... The most basic description of the plant extracellular matrix (ECM) is the cell wall, but it is actually the cell surface ... The ECM is composed of the primary and secondary cell walls, along with the intercellular gap between its neighboring cells. ... In the leaves, the MMP gene was expressed in the phloem, developing xylem elements, neighboring mesophyll cell layers, and ...
"Proliferation and differentiation of bone marrow stromal cells under hypoxic conditions". Biochemical and Biophysical Research ... It is also used to induce hypoxia-like environment in mesenchymal stem cells. Chelation therapy "Deferoxamine Mesylate". The ... Deferoxamine may modulate expression and release of inflammatory mediators by specific cell types. Deferoxamine is being ... "Iron chelator triggers inflammatory signals in human intestinal epithelial cells: Involvement of p38 and extracellular signal- ...
"Retinoic acid suppresses in-vitro decidualization of human endometrial stromal cells". Molecular Human Reproduction. 2 (3): 185 ... Structures such as the limbs, hindbrain and cranial neural crest cells have been shown to be excessively sensitive to high ... cells that produce large amounts of retinoic acid and may play a role in mediating the effects of retinoic acid in the cell. A ... Cytogenetics and Cell Genetics. 76 (1-2): 17-8. doi:10.1159/000134502. PMID 9154115. Brar AK, Kessler CA, Meyer AJ, Cedars MI, ...
25-Hydroxyvitamin D3 1-alpha-hydroxylase
Zhou S, LeBoff MS, Glowacki J (Jan 2010). "Vitamin D metabolism and action in human bone marrow stromal cells". Endocrinology. ... immune cells, and bone (osteoblasts). The enzyme catalyzes the hydroxylation of Calcifediol to calcitriol (the bioactive form ... T cell attraction to the epidermal chemokine CCL27" (PDF). Nature Immunology. 8 (3): 285-93. doi:10.1038/ni1433. PMID 17259988 ... "1alpha-hydroxylase and innate immune responses to 25-hydroxyvitamin D in colonic cell lines". The Journal of Steroid ...
"MicroRNA-494 downregulates KIT and inhibits gastrointestinal stromal tumor cell proliferation". Clin. Cancer Res. 17 (24): 7584 ... "MicroRNA-494 suppresses cell proliferation and induces senescence in A549 lung cancer cells". Cell Prolif. 45 (1): 32-8. doi: ... Cell. Biol. 31 (20): 4219-31. doi:10.1128/MCB.05955-11. PMC 3187287 . PMID 21859890. Megiorni F, Cialfi S, Dominici C, ... Cell Cycle. 11 (14): 2729-38. doi:10.4161/cc.21105. PMC 3409013 . PMID 22785131. Altmäe S, Martinez-Conejero JA, Esteban FJ, ...
Aspden RM, Scheven BA, Hutchison JD (2001). "Osteoarthritis as a systemic disorder including stromal cell differentiation and ... mammary epithelial cells, bone marrow,gastric chief cells and P/D1 cells. Leptin circulates in blood in free form and bound to ... as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells ... Location of action Leptin acts directly on leptin receptors in the cell membrane of different types of cells in the human body ...
Prostaglandin EP3 receptor
... stromal cells, endothelial cells, and, in pregnancy, placenta, chorion, and amnion); mouth gingival fibroblasts; and eye ( ... "Prostaglandin E2 regulates aromatase activity and expression in human adipose stromal cells via two distinct receptor subtypes ... media and endothelial cells of arteries and arterioles); stomach (vascular smooth muscle and gastric fundus mucosal cells); ... The growth and metastasis of implanted Lewis lung carcinoma cells, a mouse lung cancer cell line, is suppressed in EP33 ...
Mer esgyrn - Wicipedia
Gyda bodau dynol, mae Cell goch y gwaed yn cael eu cynhyrchu gan rhan tu mewn i'r mer esgyrn ym mhen esgyrn hir mewn proses a ... Mae stroma'r mer esgyrn yn cynnwys bon-gelloedd mesenchymal (MSCau), adnabyddir hefyd fel celloedd stromal mer. Mae rhein yn ... macroffabau, sy'n cyfrannu'n sylweddol at gynhyrchiad Cell goch y gwaed, gan eu bod yn mynd a Haearn ar gyfer cynhyrchiad ... Mae'n hysbys fod MSCau yn addasu, yn vitro neu'n vivo, i osteoblastau, chondroctyeau (cell cartilag), have been shown to ...
Knee cartilage replacement therapy
"Meniscal regeneration using tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells ... Then these cells are injected into the patient. These cells are held in place by a small piece of soft tissue from the tibia, ... 10,000 cells are harvested and grown in vitro for approximately six weeks until the population reaches 10-12 million cells. ... The cells grow in self-organized spheroid matrices which are implanted via injected fluid or inserted tissue matrix. For years ...
LHCGR (Luteinizing hormone insensitivity, Leydig cell hypoplasia, Male-limited precocious puberty). *FSHR (Follicle-stimulating ... KIT (KIT Piebaldism, Gastrointestinal stromal tumor). STPK. *AMHR2 (Persistent Müllerian duct syndrome II) ... "Defective migration of neuroendocrine GnRH cells in human arrhinencephalic conditions". The Journal of Clinical Investigation ...
Various types of CD30-positive T cell lymphomas. *CD30-positive cases of the NK cell lymphoma, extranodal NK/T-cell ... Thymic stromal lymphopoietin (TSLP). *Additional cytokine receptor modulators: Emfilermin. *Lestaurtinib. *Midostaurin. * ... "Identification of Hodgkin and Sternberg-reed cells as a unique cell type derived from a newly-detected small-cell population". ... "T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor ...
... examination shows that seminomas are usually composed of either a sheet-like or lobular pattern of cells with a fibrous stromal ... A seminoma is a germ cell tumor of the testicle or, more rarely, the mediastinum or other extra-gonadal locations. It is a ... Weidner N (February 1999). "Germ-cell tumors of the mediastinum". Seminars in Diagnostic Pathology. 16 (1): 42-50. PMID ... Testicular seminoma originates in the germinal epithelium of the seminiferous tubules. About half of germ cell tumors of the ...
Platelet-derived growth factor
In experiments, it new bone fully covered skull wounds in test animals and stimulated growth in human bone marrow stromal cells ... PDGF is a potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells and glial cells. In ... "Cell Death and Control of Cell Survival in the Oligodendrocyte Lineage". Cell. 70 (1): 31-46. doi:10.1016/0092-8674(92)90531-G ... vascular smooth muscle cells and mesenchymal stem cells as well as chemotaxis, the directed migration, of mesenchymal cells. ...
Wiskott-Aldrich syndrome protein
... syndrome protein and its association with other proteins by stromal cell-derived factor-1alpha is associated with cell ... cell-cell junction. • vesicle membrane. • actin cytoskeleton. • extracellular exosome. • cytoskeleton. • cytosol. • actin ... T cell receptor signaling pathway. • T cell activation. • regulation of catalytic activity. • Rho protein signal transduction. ... Oda A, Eto K (April 2013). "WASPs and WAVEs: from molecular function to physiology in hematopoietic cells". Seminars in Cell & ...
Development of analogs of thalidomide
The secretion of IL-6 by bone marrow stromal cells (BMSC) and the secretion of the adhesion molecules VCAM-1, ICAM-1 and LFA, ... They discovered increased bone marrow angiogenesis correlates with myeloma growth and supporting stromal cells are a ... They have also been shown to cause dose dependent G0/G1 cell cycle arrest in leukemia cell lines where the analogs showed 100 ... Orphan indications include diffuse large B-cell lymphoma, chronic lymphocytic leukemia and mantle cell lymphoma. Lenalidomide ...
Strok bahasa Indonesia, ensiklopedia bebas
"Stroke and T-cells". Laboratory of Neurosciences, National Institute on Aging Intramural Research Program; Arumugam TV, Granger ... Migrasi ini dimediasi oleh beberapa senyawa antara lain eritropoietin, stromal-derived factor 1 (SDF-1) dan angiopoietin-1 ... sickle cell anemia. Trombositemia dan sejenisnya. *Hypercoaguable states-puerperium. oral contraceptive use. 'sticky platelet ... "Group of Neuroplasticity and Regeneration, Institute of Neurosciences and Department of Cell Biology, Physiology and Immunology ...
They are protein mediators typically produced by T cells to direct the immune system response by signaling between its cells. ... Thymic stromal lymphopoietin (TSLP). *Additional cytokine receptor modulators: Emfilermin. *Lestaurtinib. *Midostaurin. * ... Lymphokines are a subset of cytokines that are produced by a type of immune cell known as a lymphocyte. ... Lymphokines have many roles, including the attraction of other immune cells, including macrophages and other lymphocytes, to an ...
Common side effects may include low platelets, low white blood cells, anemia, rashes, vomiting, diarrhea, and joint pains. ... 2011 that it was discontinuing a phase III trial of nilotinib as the first-line treatment of gastrointestinal stromal tumor ( ... and reduced blood cell count. Less typical side effects are those of the cardiovascular system, such as high blood pressure, ... Nilotinib is a Bcr-Abl tyrosine kinase inhibitor and works by interfering with signalling within the cancer cell. ...
سرطان پروستات - ویکیپدیا، دانشنامهٔ آزاد
gonadal stromal. *Sertoli-Leydig cell tumour *Sertoli cell tumour. *Leydig cell tumour ... lo Prostate Cancer Cell Population Harbors Self-Renewing Long-Term Tumor-Propagating Cells that Resist Castration". Cell Stem ... LNCaP cells express androgen receptor (AR), but PC-3 and DU-145 cells express very little or no AR. AR, an androgen-activated ... Prostate cancer cells are generally devoid of zinc. This allows prostate cancer cells to save energy not making citrate, and ...
Lemmon, Mark A.; Schlessinger, Joseph (2010). "Cell Signaling by Receptor Tyrosine Kinases". Cell. 141 (7): 1117-34. doi: ... "Phase II Study of Crenolanib (CP-868,596), for the Treatment of Patients With Advanced Gastrointestinal Stromal Tumors With the ... In CHO cells expressing an activating exon 18 (D842V) PDGFRα mutation, crenolanib was effective at an IC50 of 6nM and IC90 of ... The lung cancer cell line H1703, which is reported to have amplification of both PDGFRA (4q12) and PDGFC (4q32) genes on ...
Red blood cell
The difficult step was to induce the cells to eject their nucleus; this was achieved by growing the cells on stromal cells from ... Red blood cells, also known as RBCs, red cells, red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek ... Red blood cells in mammals anucleate when mature, meaning that they lack a cell nucleus. In comparison, the red blood cells of ... Red blood cells are cells present in blood in order to transport oxygen. The only known vertebrates without red blood cells are ...
Histologically, it forms clusters of goblet cells containing mucin with a minor admixture of Paneth cells and endocrine cells. ... Goblet cell carcinoid. Main article: Goblet cell carcinoid. This is considered to be a hybrid between an exocrine and ... Carcinoid (also carcinoid tumor) is a slow-growing type of neuroendocrine tumor originating in the cells of the ... The term 'crypt cell carcinoma' has been used for them, and though perhaps more accurate than considering them carcinoids, has ...
Hepatocyte growth factor
"Stromal-epithelial cell interactions and alteration of branching morphogenesis in macromastic mammary glands". Journal of ... It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts ... endothelial cells and haemopoietic progenitor cells, HGF also regulates the chemotaxis of T cells into heart tissue. Binding of ... cell morphogenesis. • positive regulation of cell proliferation. • hyaluronan metabolic process. • positive regulation of DNA ...
Cells in the thymus can be divided into thymic stromal cells and cells of hematopoietic origin (derived from bone marrow ... Stromal cells include epithelial cells of the thymic cortex and medulla, and dendritic cells. The thymus provides an inductive ... In addition, thymic stromal cells allow for the selection of a functional and self-tolerant T cell repertoire. Therefore, one ... Second, the T cell undergoes "Negative Selection" by interacting with thymic dendritic cells, whereby T cells with high ...
... adenosine receptor agonists during proliferation and osteogenic differentiation of human primary bone marrow stromal cells". J ... The osteoblast cell is derived from the Mesenchymal Stem Cell (MSC) which can also differentiate into a chondrocyte. The ... It allows for the inhibition of growth in human melanoma cells. Specific antagonists include MRS1191, MRS1523 and MRE3008F20, ... Cell Physiol. 226 (5): 1353-1366. doi:10.1002/jcp.22458.. *^ Carroll SH, Ravid K (2013). "Differentiation of mesenchymal stem ...
Acute lymphoblastic leukemia
Mesenchymal stromal cells may results in little to no difference in the all-cause mortality, relapse of malignant disease and ... T cell or pre-B cell Large and heterogeneous (varied) cells ALL - L3 B cell Large and varied cells with vacuoles Mature B-cell ... The cancerous cell in ALL is the lymphoblast. Normal lymphoblasts develop into mature, infection-fighting B-cells or T-cells, ... Hyperdiploid cells are defined as cells with more than 50 chromosomes, while hypodiploid is defined as cells with less than 44 ...
"Stromal-epithelial cell interactions and alteration of branching morphogenesis in macromastic mammary glands". Journal of ... was found to attenuate the proliferation of breast epithelial tissue caused by exposure to macromastic breast stromal cells, ... "Cell. Signal. 19 (9): 1956-63. doi:10.1016/j.cellsig.2007.05.003. PMC 2681182. PMID 17572069.. ... Rawlings JS, Rosler KM, Harrison DA (2004). "The JAK/STAT signaling pathway". J. Cell Sci. 117 (Pt 8): 1281-3. doi:10.1242/jcs. ...
Inflammatory breast cancer
It is characterised by the presence of cancer cells in the subdermal lymphatics on skin biopsy. Consequently, IBC is always ... Fibroepithelial/stromal. *Phyllodes tumor. Ductal, lobular, and medullary. Ductal. *Ductal carcinoma in situ (DCIS): Paget's ... caveolin-1 and -2 are overexpressed and may contribute to tumour cell motility ... 2 in cell lines and in human samples of inflammatory breast cancer". Breast Cancer Research and Treatment. 95 (3): 219-28. doi: ...
പ്രോസ്റ്റാഗ്ലാൻഡിൻ EP3 റിസപ്റ്റർ - വിക്കിപീഡിയ
"Prostaglandin E2 regulates aromatase activity and expression in human adipose stromal cells via two distinct receptor subtypes ... cell death. • signal transduction. • negative regulation of gastric acid secretion. • phospholipase C-activating G-protein ... "Induction of IL-6 via the EP3 subtype of prostaglandin E receptor in rat adjuvant-arthritic synovial cells". Inflammation ... "Cloning and expression of a prostaglandin E receptor EP3 subtype from human erythroleukaemia cells". The Biochemical Journal ...
Non-hematopoietic mesenchymal cells ⇨ sarcoma. *Hematopoietic cells *Bone marrow-derived cells that normally mature in the ... The appearance of the local tissue and stromal architecture. *The anatomical location from which tumors arise ... Composed of large, monotonous rounded or overtly polygonal-shaped cells with abundant cytoplasm.. Small cell carcinoma. Cells ... Certain combinations of mutations in the given progenitor cell ultimately result in that cell (also called a cancer stem cell) ...
Stromal cells are the cells that support the parenchymal cells in any organ. Fibroblasts, immune cells, pericytes, and ... Cell damage (also known as cell injury) is a variety of changes of stress that a cell suffers due to external as well internal ... When a cell is damaged the body will try to repair or replace the cell to continue normal functions. If a cell dies the body ... Asexual reproduction is what repairs cells Regeneration. Regeneration of parenchyma cells, or the functional cells, of an ...
These ROS drive cancer cell proliferation by activating kinases that drive cell cycle progression growth factors at low ... Kim HS, Lee HE, Yang HK, Kim WH (2014). "High lactate dehydrogenase 5 expression correlates with high tumoral and stromal ... Lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH ... In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a ...
Magnesium in biology
Cell membranes and wallsEdit. Biological cell membranes and cell walls are polyanionic surfaces. This has important ... Measurement of free Mg2+ by using a fluorescent probe and intensity of stromal alkalinization". Archives of Biochemistry and ... it is generally assumed that the ion stays in that cell for as long as the cell is active. In vascular cells, this is not ... The concentration of Mg2+ in the root cells is probably buffered by storage in root cell vacuoles (3). Note that cells in the ...
Dana-Farber Cancer Institute
They also are among the first to suspect a relationship between the retrovirus that causes human T-cell leukemia (HTLV-1) and ... eliminate tumors in some patients with a rare and otherwise incurable digestive-tract cancer called gastrointestinal stromal ... 2005: Dana-Farber scientists report that the drug gefitinib (Iressa) produces dramatic benefits in non-small cell lung cancer ... replicates and infects healthy cells. Science magazine heralds this discovery as its "Breakthrough of the Year". 1998: A drug ...
Mesenchymal Stromal Cell Therapy of Stroke | SpringerLink
Bone marrow stromal cells and bone marrow-derived mononuclear cells: which are suitable as cell source of transplantation for ... Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J Cell ... Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol. 2000;164(2):247-56.PubMedCrossRefGoogle ... Human bone marrow stromal cell: coexpression of markers specific for multiple mesenchymal cell lineages. Blood Cells Mol Dis. ...
Mesenchymal Stromal Cells and Viral Infection
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... Mesenchymal Stromal Cells (MSCs) are a subset of nonhematopoietic adult stem cells, readily isolated from various tissues and ... Mesenchymal Stromal Cells (MSCs) are nonhematopoietic stem cells which have high proliferation, self-renewal, and multilineage ...
Stromal cell - Wikipedia
Fibroblasts and pericytes are among the most common types of stromal cells. The interaction between stromal cells and tumor ... bone marrow stromal cells have been described to be involved in human haematopoiesis and inflammatory processes. Stromal cells ... Stromal cells are connective tissue cells of any organ, for example in the uterine mucosa (endometrium), prostate, bone marrow ... cannot spread throughout the body because the cancer cells require nearby stromal cells to continue their division. The loss of ...
Mesenchymal Stromal Cell-Derived ... preview & related info | Mendeley
Mesenchymal stromal cell-(MSC-) secreted factors are described to stimulate regeneration after par... ... Mesenchymal Stromal Cell-Derived Factors Promote Tissue Repair in a Small-for-Size Ischemic Liver Model but Do Not Protect ... Mesenchymal stromal cell-derived factors promote tissue regeneration of small-for-size livers exposed to ischemic conditions ... Mesenchymal stromal cell-(MSC-) secreted factors are described to stimulate regeneration after partial hepatectomy. This study ...
Management of Fibrosis: The Mesenchymal Stromal Cells Breakthrough
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... A. Keating, "How do mesenchymal stromal cells suppress T cells?" Cell Stem Cell, vol. 2, no. 2, pp. 106-108, 2008. View at ... Among these, cell therapy is regarded as one of the best candidates. In particular, mesenchymal stromal cells (MSCs) have great ...
Stromal cell-derived factor - Wikipedia
Stromal Cell Ultrastructure | IntechOpen
Human bone marrow mesenchymal stem/stromal cells (MSCs) have great potential for cellular therapy, as they possess the ... Stem cells are capable of self-renewal and multilineage differentiation. They also have the ability to create immunomodulatory ... MSCs are present in all tissues interacting with tissue cells and easy to isolate and expand in culture. Indeed, histological ... Thus, the aim of this chapter is to give a spot of light on these cells and their histology. ...
SDF2 stromal cell derived factor 2 [Homo sapiens (human)] - Gene - NCBI
SDF2 stromal cell derived factor 2 [Homo sapiens] SDF2 stromal cell derived factor 2 [Homo sapiens]. Gene ID:6388 ... Genes Cells, 2017 Aug. PMID 28597544 * Isolation and characterization of a novel secretory protein, stromal cell-derived factor ... Stromal Cell-Derived Factor 2: A Novel Protein that Interferes in Endoplasmic Reticulum Stress Pathway in Human Placental Cells ... Protection of stromal cell-derived factor 2 by heat shock protein 72 prevents oxaliplatin-induced cell death in oxaliplatin- ...
Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells. - PubMed - NCBI
... cells and at least three subsets of stromal cells that exhibit differential expression of cell surface markers, including CD105 ... generates stromal colonies that support HSCs. PI(−)CD45(−)Tie2(−)CD51(+)CD105(+)Thy(−)6C3(−) cells were single-cell sorted from ... labeled hematopoietic stem cell (arrowhead) homes to GFP(+) stromal cells in ectopic bone from transplantation of population a- ... Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells.. Chan CK1, Lindau P, Jiang W, Chen JY, Zhang LF, ...
Thymic stromal lymphopoietin drives the development of IL-13+ Th2 cells | PNAS
... type 2 innate lymphoid cells (ILC2), mast cells, basophils, and T cells (14, 16). The direct effects of TSLP on T cells have ... 3 B and C). No IL-13DR SP cells were found in cultures of IL-4AC SP cells, suggesting that IL-4AC SP and IL-13DR SP cells ... On day 2, at the peak of IL-4AC expression, cells were sorted into AC-positive and AC-negative cells (Fig. 3A). Cells were ... To determine whether IL-13DR SP cells arise from cells that were previously IL-4AC SP, naive CD4+ T cells were sorted and ...
Patent US5478739 - Three-dimensional stromal cell and tissue culture system - Google Patents
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three- ... observing and studying cells in culture, allowing a high rate of cell proliferation, it lacks the cell-cell and cell-matrix ... After reaching near-confluency the stromal cells are inoculated with tumor cells. The tumor cells will continue to divide ... Such cells include but are not limited to endothelial cells, pericytes, macrophages, monocytes, plasma cells, mast cells, ...
Inferring tumour purity and stromal and immune cell admixture from expression data | Nature Communications
Here we describe Estimation of STromal and Immune cells in MAlignant Tumours using Expression data (ESTIMATE)-a method that ... uses gene expression signatures to infer the fraction of stromal and immune cells in tumour samples. ESTIMATE scores correlate ... The ESTIMATE method allows consideration of tumour-associated normal cells in genomic and transcriptomic studies. An R-library ... Infiltrating stromal and immune cells form the major fraction of normal cells in tumour tissue and not only perturb the tumour ...
Frontiers | Mesenchymal Stromal Cells for Transplant Tolerance | Immunology
Cellular therapy with mesenchymal stromal cells (MSC) has recently emerged as a promising strategy to regulate anti-donor ... including down-regulation of effector T-cell response and activation of regulatory pathways. We will also provide an overview ... highlighting the issues that still need to be addressed before establishing MSC as a safe and effective tolerogenic cell ... including down-regulation of effector T-cell response and activation of regulatory pathways. We will also provide an overview ...
Stromal Cells in Immunity and Disease 20Q5 - Summary
For instance, stromal cell dysregulation has been seen in HIV infection and in cancer patients. Furthermore, stromal cells are ... stromal cells support the migration and interactions between antigen presenting cells and naïve T and B cells during the ... Stromal Cells in Immunity and Disease. joint with Fibrosis and Tissue Repair: From Molecules and Mechanics to Therapeutic ... Our understanding of stromal cell populations and their contributions to innate and adaptive immunity as well as immunological ...
JCI - Stromal cell cadherin-11 regulates adipose tissue inflammation and diabetes
Cell-surface cadherin-11 expression on CD45-CD235α-CD31- cells in stromal vascular cells isolated from obese human omentum fat ... Stromal cells regulate leukocyte responses in lymph nodes, but the role of stromal cells in adipose tissue inflammation is ... M2 macrophages, innate lymphoid type 2 cells (ILC2s), eosinophils, Tregs, and invariant NK T cells (iNKT cells) all help to ... PDGFRα+ stromal cells are major producers of IL-33 in adipose tissue. Here, we show that mesenchymal cadherin-11 modulates ...
Downregulation of CXCL12 in mesenchymal stromal cells by TGFβ promotes breast cancer metastasis | Oncogene
In presence of CXCL12, CXCR7 expression on tumour cells was decreased. Indeed, when CXCR7 was silenced in breast cancer cells, ... We found that 4T1 breast cancer cells expressed high levels of CXCR7, but not of CXCR4, both of which are CXCL12 receptors. ... Our findings reveal a novel mechanism of MSC effects on malignant cells through which crosstalk between MSCs and TGFβ regulates ... this CXCL12 effect of MSCs on tumour cells is relieved. Importantly, elevated CXCR7 and depressed CXCL12 expression levels were ...
Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacity | PNAS
2013) Onset of heterogeneity in culture-expanded bone marrow stromal cells. Stem Cell Res (Amst) 11(3):1365-1377. ... 2014) Gene markers of cellular aging in human multipotent stromal cells in culture. Stem Cell Res Ther 5(2):59. ... 2014) IDO-independent suppression of T cell effector function by IFN-γ-licensed human mesenchymal stromal cells. J Immunol 192( ... 2016) Identification of predictive gene markers for multipotent stromal cell proliferation. Stem Cells Dev 25(11):861-873. ...
Proliferation of Endometrial Stromal Cells in Adenomyosis - Full Text View - ClinicalTrials.gov
Proliferation of Endometrial Stromal Cells in Adenomyosis. The safety and scientific validity of this study is the ... Our further investigation revealed that in vitro coculture of macrophages and endometrial stromal cells (ESCs) increase the ... Eutopic endometrium was obtained and separated into single endometrial stromal cell (ESC) in women with adenomyosis (study ... Quantification of cell proliferation was done with Cell Proliferation Assay Kit and immunocytochemical detection of Ki-67, in ...
JCI - Dendritic cells maintain dermal adipose-derived stromal cells in skin fibrosis
Adipose tissue contains adipose-derived mesenchymal stromal cells (ADSCs) that have regenerative and reparative functions; ... and suggest an approach for improving mesenchymal stromal cell therapy in scleroderma and other diseases. ... Here, we have shown that DWAT ADSC numbers were reduced, likely because of cell death, in 2 murine models of scleroderma skin ... The remaining ADSCs showed a partial dependence on dendritic cells (DCs) for survival. Lymphotoxin β (LTβ) expression in DCs ...
Keystone Symposia Conference | Stromal Cells in Immunity and Disease - Program
Mesenchymal Stromal Cells in Living Donor Kidney Transplantation - Full Text View - ClinicalTrials.gov
Experimental: Mesenchymal Stromal Stem Cells Infusion Intervention: Mesenchymal stromal stem cells infusion. This is the active ... Mesenchymal Stromal Stem Cells (MSCs) Infusion Investigational infusion bag containing autologous mesenchymal stromal cells ... Renal Transplantation Mesenchymal Stem Cells Biological: Mesenchymal Stromal Stem Cells (MSCs) Infusion Other: Normal Saline ( ... 6 cells for the first dose group, 2x10^6 cells for the second dose group, or 3x10^6 cells for the last dose group. The infusion ...
NIOSHTIC-2 Publications Search - 00141061 - In vitro effects of benzene metabolites on mouse bone marrow stromal cells.
Bone marrow cell suspensions were prepared from male B6C3F1-mice and cultured with hydroquinone (123319), benzoquinone (106514 ... metabolites on bone marrow stromal cells were investigated in-vitro. ... The effects of benzene (71432) metabolites on bone marrow stromal cells were investigated in-vitro. Bone marrow cell ... Stromal cells exposed to metabolites at these same concentrations were also cultured simultaneously with fresh bone marrow ...
Secretome protein signature of human gastrointestinal stromal tumor cells. | Sigma-Aldrich
Experimental cell research 2015-5-20 Secretome protein signature of human gastrointestinal stromal tumor cells.. [Erik Berglund ... Herein, we stimulated cell secretion in the imatinib-sensitive GIST882 cell line and profiled the secretome, collected as ... In spite of major improvements in clinical management, gastrointestinal stromal tumors (GISTs) can still be deadly due to ...
Immunological Hallmarks of Stromal Cells in the Tumour Microenvironment
A dynamic and mutualistic interaction between tumour cells and the surrounding stroma promotes the initiation, progression, ... Immunological Hallmarks of Stromal Cells in the Tumour Microenvironment Nat Rev Immunol. 2015 Nov;15(11):669-82. doi: 10.1038/ ... with emphasis on the immunological attributes of stromal cells that may foster their protumorigenic function. ... In this Review, we discuss the evidence that stromal determinants interact with leukocytes and influence antitumour immunity, ...
UA professor receives NSF grant to study role of stromal cells in breast cancer
... to study the role of stromal cells in cancer. ... cells and cancer-associated fibroblasts as stromal cells. ... In addition to a mass of cancer cells, tumors contain several other types of cells known collectively as stromal cells. These ... Treatments have only focused on the cancerous cells and largely neglected the stromal cells that contribute to tumor growth and ... Our studies using this model will allow us to mechanically understand how stromal cells render cancer cells proliferative and ...
Autologous Stem/Stromal Cells in Neurological Disorders and Disease - Full Text View - ClinicalTrials.gov
Autologous Stem/Stromal Cells in Neurological Disorders and Disease (NDD). The safety and scientific validity of this study is ... The study deals with evaluation of safety and efficacy of use of stem/stromal cell isolates from autologous microvasculature in ... These cells are suspending in sterile Normal Saline Solution (500cc) and re-administered via an intravenous parenteral route, ... Autologous cells are acquired via microcannula aspiration of subdermal fat deposits, isolated through a digestive process, and ...
'mesenchymal stromal cells' Protocols and Video...
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells, Mesenchymal Stromal Cell Culture and ... mesenchymal stromal cells include Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous ... A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro, Construction of Defined Human ... and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice, In Vivo Tracking of Human Adipose- ...
Frontiers | Mesenchymal Stromal Cell Dependent Regression of Pulmonary Metastasis from Ewing's | Pediatrics
Mesenchymal stromal cell (MSC) therapy has shown potential benefit for Kaposis sarcoma; however, the role of progenitor cell ... Within this model, we demonstrate the use of MSCs to target ES lung metastasis.Materials and Methods: Human ES cells were ... Materials and MethodsHuman ES cells were transfected with luciferase and injected into the rib of nude mice. Development of ... the role of progenitor cell therapies for cancer remains controversial. MSC treatment of ES or pulmonary metastatic disease has ...
PatientsLikeMe | Adipose tissue-derived stromal cell transplant report for patients like you
Find the most comprehensive real-world treatment information on Adipose tissue-derived stromal cell transplant at ... bipolar I disorder or psoriasis currently have Adipose tissue-derived stromal cell transplant. ... What is Adipose tissue-derived stromal cell transplant?. Category: Procedures false As stem cells can be grown and transformed ... into specialized cells with characteristics consistent with cells of various tissues such as muscles or nerves, their use in ...
Anti-Bone marrow stromal cell antigen 1 antibody (ab137718) | Abcam
Rabbit polyclonal Bone marrow stromal cell antigen 1 antibody. Validated in WB, IHC and tested in Human. Cited in 1 publication ... Anti-Bone marrow stromal cell antigen 1 antibody. See all Bone marrow stromal cell antigen 1 primary antibodies. ... Anti-Bone marrow stromal cell antigen 1 antibody (ab137718) at 1/500 dilution + Raji whole cell lysate at 30 µg. Predicted band ... Protein - Recombinant Human Bone marrow stromal cell antigen 1 protein (His tag) (ab235881) SDS-PAGE ...
DifferentiationMSCsFibroblastsAbstractVitroProliferationStemTissueMarrow Stromal CellsMicroenvironmentEndothelial cellsTumorsCytokineAntigenProgenitor cellsThymicTracking of mesenchymal stromal cellsTissuesMacrophagesMesenchymal Stromal Cell-DerivedDendritic cellsMultipotentFibroblasticTypes of stromal cellsRole of stromal cellsEpithelial and stromal cellsResults suggest that stromalEndometrial stromal cellInflammatoryHuman boneUmbilicalLymphoidVivoFibroblastStromaVascular cellsExpression in stromalInteractionsGastrointestinal stromBone marrow-derived mesenchymalRegulateCulturesHematopoietic cellsAutologousGrowthImmune cellsTransplantationExtracellular matrixPopulationsPrimary stromalPrecursors
- Mesenchymal Stromal Cells (MSCs) are nonhematopoietic stem cells which have high proliferation, self-renewal, and multilineage differentiation capabilities. (hindawi.com)
- Stem cells are capable of self-renewal and multilineage differentiation. (intechopen.com)
- Thus, TSLP drives the early differentiation of a distinct population of effector Th2 cells with pro-inflammatory properties. (pnas.org)
- Differentiation of S-V cells was found to be under hormonal control. (oregonstate.edu)
- Insulin and glucocorticoids are essential for S-V cell differentiation in culture. (oregonstate.edu)
- Serum source (newborn vs mature) did not affect differentiation of S-V cells from newborn or mature pig adipose tissue. (oregonstate.edu)
- When sera from fed or fasted pigs were used to culture newborn pig S-V cells, fasted pig sera stimulated greater differentiation and decreased cell replication as indicated by DNA content of rat S-V cell culture. (oregonstate.edu)
- Lean pig serum compared to obese pig serum, increased differentiation activity in culture of S-V cells an effect which may be influenced by sex. (oregonstate.edu)
- Rat serum fraction two (apparent molecular size 67-150 kD) promoted greater differentiation of S-V cells than other rat serum fractions or pig serum fraction two. (oregonstate.edu)
- Fraction three (apparent molecular size 17-43 kD) of both sera inhibited differentiation and lipid filling in cultures of S-V cells but only rat fraction three promoted cell proliferation. (oregonstate.edu)
- Our study establishes Nrf2/Keap1 as a cytoprotective pathway, as well as a metabolic rheostat that affects cell maintenance and differentiation switches in BMSCs. (diabetesjournals.org)
- Illmensee K, Mintz B. Totipotency and normal differentiation of single teratocarcinoma cells cloned by injection into blastocysts. (springer.com)
- Chemical induction of osteoblastic differentiation from BM stromal cells also induced an increase in FL production. (irsn.fr)
- Aim of this study was to compare MSC from porcine bone marrow (BM) with human cells for phenotype, multi-lineage differentiation potential, immune-modulatory capacity and the effect on cardiac function after transplantation in a mouse model of myocardial infarction. (wiley.com)
- Additionally, we successfully showed that by using the pLenti7.3 vector it is possible to efficiently over-express different growth factors, particularly relevant for cardiac protection and differentiation, in human mesenchymal stromal cells. (ovid.com)
- To date these cells have been investigated for their differentiation potential and are currently being used to treat damage to horse musculoskeletal tissues. (dovepress.com)
- Endosialin expression by both CAF and MSC further implies the potential contribution of MSC to tumor stroma via differentiation into tumor stromal fibroblasts. (spandidos-publications.com)
- Megakaryopoiesis is the hierarchical differentiation of hematopoietic stem cells into megakaryocytes. (bloodjournal.org)
- Megakaryopoiesis is a hierarchical differentiation process from hematopoietic stem cells to megakaryocytes, which culminates in platelet production. (bloodjournal.org)
- It is well known that the ceramics structure and composition affect cell proliferation / differentiation. (scientific.net)
- In this study, three different types of HA ceramics were used to investigate initial cell attachment followed by osteoblastic differentiation of human mesenchymal stromal cells (MSCs). (scientific.net)
- In the present study, we explored the role of the interferon-inducible protein p202 in osteoblast differentiation of mouse bone marrow stromal cells (BMSCs). (portlandpress.com)
- The osteogenic potential of bone marrow stromal cells (BMSCs) makes them selected tools for the investigation of the involved factors during osteoblast proliferation and differentiation [ 5 ]. (portlandpress.com)
- They expressed in many tissues and act as important regulators in cell growth, apoptosis, immunomodulation, and tissue-specific differentiation [ 6-8 ]. (portlandpress.com)
- p202, one of the most extensively studied p200 family members, functioned in cell-cycle regulation and differentiation via interaction with some transcription factors [ 9-11 ]. (portlandpress.com)
- For instance, Id (inhibitor of differentiation) proteins act as important regulators in cell growth and differentiation [ 12 , 13 ]. (portlandpress.com)
- These colony-forming unitfibroblasts (CFU-Fs) were capable of osteogenic differentiation and provided the first evidence of a clonogenic precursor for cells of the bone lineage. (stemcell.com)
- 25 Pericytes and adventitial cells also natively express mesenchymal markers and share similar gene expression profiles as well as developmental and differentiation potential with mesenchymal cells. (stemcell.com)
- This study is the first to explore multi-lineage differentiation of a stem cell line and primary stem cells within poly( N -isopropylacrylamide) (p(NIPAAm))-based thermosensitive microgel-formed constructs. (rsc.org)
- Due to functional enhancement of cell spheroids, hMSCs within the 3D microgel-formed constructs were induced for multi-lineage differentiation as evidenced by significant up-regulation of messenger RNA (mRNA) expression of chondrogenic and osteogenic genes even in the absence of induction media on day 9. (rsc.org)
- Hence, the thermosensitive p(NIPAAm-AA) microgel can be potentially used in an in vitro model for cell differentiation or in vivo transplantation of pre-differentiated human mesenchymal stromal cells into patients for specific lineage differentiation. (rsc.org)
- 2005) Gene expression profile of cytokine and growth factor during differentiation of bone marrow derived mesenchymal stem cell, Cytokine, 31, 119-126. (scirp.org)
- In addition, the role of microRNAs (miRNAs) and MSC secreted exosomes in mediating intercellular communication between MSCs and parenchymal cells of the brain, and their effects on the regulation of neurovascular remodeling and white matter remodeling after stroke are discussed. (springer.com)
- Mesenchymal Stromal Cells (MSCs) are a subset of nonhematopoietic adult stem cells, readily isolated from various tissues and easily culture-expanded ex vivo . (hindawi.com)
- However, the interplay between MSCs and virus is like a double-edge sword, and it also provides beneficial effects such as allowing the proliferation and function of antiviral specific effector cells instead of suppressing them, serving as an ideal tool for study of viral pathogenesis, and protecting hosts against viral challenge by using the antimicrobial activity. (hindawi.com)
- Here, we therefore review favorable and unfavorable consequences of MSCs and virus interaction with the highlight of safety and efficacy for applying MSCs as cell therapy. (hindawi.com)
- Given the immunomodulatory activity of MSCs, together with their low MHC class I expression, MSCs have been utilized to prevent and/or treat steroid-resistant graft-versus-host disease (GvHD) in patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) that have failed treatment with conventional immunosuppressant drugs. (hindawi.com)
- In particular, mesenchymal stromal cells (MSCs) have great potential in the treatment of inflammatory diseases. (hindawi.com)
- Human bone marrow mesenchymal stem/stromal cells (MSCs) have great potential for cellular therapy, as they possess the abilities to proliferate as well as to differentiate. (intechopen.com)
- MSCs are present in all tissues interacting with tissue cells and easy to isolate and expand in culture. (intechopen.com)
- This mesodermal cell layer contains mesenchymal stem cells (MSCs), which develop into connective tissue (mesenchyme) and it maintains the progenitor stem cells that persist after birth [ 3 ]. (intechopen.com)
- Mesenchymal stem or stromal cells (MSCs) are multipotent cells that play a pivotal role in various phases of lung development and lung homeostasis, and potentially also lung regeneration. (rug.nl)
- MSCs do not only self-renew and differentiate into renew tissues, but also have anti-inflammatory and paracrine properties to reduce damage and to support tissue regeneration, constituting a promising cell-based treatment strategy for the repair of damaged alveolar tissue in emphysema. (rug.nl)
- This review will provide a comprehensive overview of the (pre)clinical studies on MSC effects in emphysema and discuss the current challenges regarding the optimal use of MSCs for cell-based therapies. (rug.nl)
- Mesenchymal stromal cells (MSCs) are one of major components of the tumour microenvironment. (nature.com)
- Therefore, our data demonstrated that sustained expression of CXCL12 by MSCs in the primary tumour site inhibits metastasis through reduction of CXCR7, while, in the presence of TGFβ, this CXCL12 effect of MSCs on tumour cells is relieved. (nature.com)
- Our findings reveal a novel mechanism of MSC effects on malignant cells through which crosstalk between MSCs and TGFβ regulates tumour metastasis. (nature.com)
- Many types of cells, such as immune cells, endothelial progenitor cells and mesenchymal stromal cells (MSCs), are recruited to the stroma of developing tumours. (nature.com)
- 8 Experimental evidence has shown that the direct effects of MSCs on tumour cells can enhance tumour metastasis. (nature.com)
- The complexity of MSCs in regulating different types of tumour development, such as breast cancer, was associated with their ability to secrete plentiful growth factors that support tumour growth and angiogenesis, as well as their role in moulding the tumour immune microenvironment by facilitating monocyte and macrophage infiltration, and suppressing anti-tumour T-cell activity. (nature.com)
- The contributions of MSCs to breast cancer metastasis are mostly mediated through their ability to produce a number of factors, such as CCL5, CXCL12, which in turn exert paracrine actions on breast cancer cells that resulted in their invasion and/or distant organ metastasis. (nature.com)
- Substantial evidence exists demonstrating the immunosuppressive function of mesenchymal stromal cells (MSCs), but inconsistent clinical results suggest that better understanding of MSC-mediated immunosuppression and identification of features predictive of immunosuppressive capacity would advance MSC-based therapeutics. (pnas.org)
- To identify features associated with immunosuppressive capacity in MSCs, we developed a robust in vitro assay that uses principal-component analysis to integrate multidimensional flow cytometry data into a single measurement of MSC-mediated inhibition of T-cell activation. (pnas.org)
- Human mesenchymal stromal cells (MSCs) can potently suppress immune responses in vitro and in animal models of human disease ( 1 , 2 ), but to date MSC-based therapies have produced mixed results in clinical trials for treatment of inflammatory and autoimmune diseases ( 3 , 4 ). (pnas.org)
- This research study will evaluate the safety and activity of mesenchymal stromal stem cells (MSCs) infusion compared to saline-only infusion in reducing the immune suppression necessary to achieve optimal renal function in renal transplant recipients. (clinicaltrials.gov)
- The investigator will obtain exploratory immune response markers to estimate the effect of autologous MSCs on the T- and B-cell response following living donor kidney transplantation. (clinicaltrials.gov)
- Phase II Clinical Trial to Assess the dose-response and Efficacy of Umbilical Cord-derived Mesenchymal Stromal Cells (MSCs) in Severe Renal Systemic Lupus Erythematosus (SLE). (clinicaltrials.gov)
- Mesenchymal stromal cells (MSCs) are currently being investigated for use in a wide variety of clinical applications. (semanticscholar.org)
- Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. (semanticscholar.org)
- Mesenchymal stromal cells (MSCs) are inherently immunomodulatory through production of inhibiting soluble factors and expression of immunosuppressive cell surface markers. (frontiersin.org)
- To assess whether activated MSC can modulate adaptive immunity, MSCs were pulsed with islet auto-antigen (GAD65) peptide to stimulate GAD65-specific T-cells. (frontiersin.org)
- Activated HLA-DR3-expressing MSCs pulsed with GAD65 peptide inhibited proliferation of HLA-DR3-restricted GAD65-specific T-cells, while this HLA class II expression did not induce cellular alloreactivity. (frontiersin.org)
- Conditioning of antigen-specific T-cells by activated and antigen-pulsed MSCs prevented T-cells to proliferate upon subsequent activation by dendritic cells, even after removal of the MSCs. (frontiersin.org)
- In sum, activation of MSCs with inflammatory stimuli turns these cells into suppressive cells capable of mediating adaptive regulation of proinflammatory pathogenic T-cells. (frontiersin.org)
- Mesenchymal stromal cells (MSCs) are non-hematopoietic cells that can easily be sourced from various tissues, including bone marrow ( 1 ). (frontiersin.org)
- Interestingly, MSCs have also been investigated for their potential to regenerate beta-cells, or to contribute to regeneration of beta-cells, which is another strategy to counter T1D ( 5 , 6 ). (frontiersin.org)
- Although mesenchymal stromal cells (MSCs) have been applied clinically to treat cardiac diseases, it is unclear how and to which extent transplanted MSCs exert their beneficial effects. (wiley.com)
- We detected an Epstein Barr virus (EBV) associated B cell lymphoproliferative lesion in the rectum of a patient 4 years after local administration of MSCs for his perianal fistulas. (rug.nl)
- Insception Lifebank's Scientific Director, Dr. Rogers, explains in this short animation how Mesenchymal Stromal Cells (MSCs) and especially those derived from umbilical cord tissue, are showing exciting potential to treat COVID-19. (insception.com)
- MSCs), with the latter responsible for the maintenance of the non-hematopoietic bone marrow cells. (stemcell.com)
- MSCs, also termed multipotent marrow stromal cells or mesenchymal stromal cells, are a heterogeneous population of plastic-adherent, fibroblast-like cells, which can self-renew and differentiate into bone, adipose and cartilage tissue in culture. (stemcell.com)
- 10-18 Accumulating evidence indicates a perivascular location for these MSC-like cells in all tissues, implying that all MSCs are pericytes 19 that closely encircle endothelial cells in capillaries and microvessels in multiple organs. (stemcell.com)
- Mesenchymal stromal cells (MSCs) play an important role in tissue repair and maintenance and provide an attractive candidate for cell-based therapies. (arvojournals.org)
- Mesenchymal stromal cells (MSCs) are adult stem cells, found in situ within all adult mammalian supportive stromal tissue compartments, where they play a key role in the organization and maintenance of tissue integrity and in the physiological/pathological tissue repair . (thefreelibrary.com)
- ECM composition and remodelling depend in part on the activity of proteolytic enzymes that selectively digest individual components of the matrix, matrix metalloproteinases (MMPs), which are constitutively expressed by a wide range of cell types, including BM-MSCs, MSCs from adipose tissue and from other tissues [14, 18- (thefreelibrary.com)
- We have recently demonstrated that BM-MSCs are also able to produce S1P through sphingosine kinase (SphK) activation and to release it similarly to many other cell types . (thefreelibrary.com)
- Fibroblasts and pericytes are among the most common types of stromal cells. (wikipedia.org)
- Therefore, human tumor-derived stromal fibroblasts are slowly lost during PDX tumor expansion in mice and concomitantly replaced by mouse-derived stromal fibroblasts (see figure 1). (jax.org)
- Ultrastructurally, most of these cells appeared to be exclusively fibroblasts. (springer.com)
- The present study showed that the typical stromal cells in nasopharyngeal angiofibromas were fibroblasts and not myofibroblasts. (springer.com)
- These results suggest that stromal fibroblasts expressing cadherin-11 regulate adipose tissue inflammation and thus highlight cadherin-11 as a potential therapeutic target for the management of obesity. (jci.org)
- A ) Representative flow cytometric plots of cell-surface cadherin-11 (Cad11) expression on CD45 - Ter119 - CD31 - PDGFR + fibroblasts among SVF cells in eWAT from WT and cad-11 -/- mice. (jci.org)
- As a disease model, this project will use triple negative breast cancer (TNBC) cells and cancer-associated fibroblasts as stromal cells. (news-medical.net)
- Notably, MSC can inhibit the anti-tumor immune response through either carcinoma-associated fibroblasts or bone marrow stromal cells. (mdpi.com)
- Cancer growth and metastasis are regulated in part by stromal cells such as fibroblasts and immune cells within the tumor microenvironment. (sciencemag.org)
- The tumor microenvironment comprises a mass of heterogeneous cell types, including immune cells, endothelial cells, and fibroblasts, alongside cancer cells. (springer.com)
- Coculture of HCA-7 cells with hereditary nonpolyposis CRC fibroblasts, but not normal fibroblasts, markedly reduced butyrate-induced apoptosis of HCA-7 cells. (aacrjournals.org)
- Cancer-associated fibroblasts (CAF) have been implicated in promoting tumor development and have been associated with mesenchymal stem cells (MSC). (spandidos-publications.com)
- Both cell types survived in the lesion site with fibroblasts displaying a larger graft volume. (wingsforlife.com)
- The bone marrow (BM) stroma contains a heterogeneous population of cells, including endothelial cells, fibroblasts, adipocytes and osteogenic cells, and it was initially thought to function primarily as a structural framework upon which hematopoiesis occurs. (stemcell.com)
- Downregulation of tenascin expression by glucocorticoids in bone marrow stromal cells and in fibroblasts. (rupress.org)
- Studies with a stromal cell line (MC3T3-G2/PA6) and fibroblasts (3T3) suggested that glucocorticoids act directly on the stromal cells that produce tenascin. (rupress.org)
- Gorskaya Yu.F., Mezentseva M.V., Shapoval I.V., Danilova T.A.et al (2009) The influence of bacterial cell preparation , IFN- ,and MIF on cytokine gene expression in passage stromal fibroblasts of human bone marrow in vitro and cells of mouse bone marrow and spleen in primary cultures, New Horizons in Allergy, Asthma & Immunology MEDIMOND International Proceedings Proceedings of the 11 World Asthma & Cord Forum SP (Russia), 205-208. (scirp.org)
- Our further investigation revealed that in vitro coculture of macrophages and endometrial stromal cells (ESCs) increase the expression of IL-6 mRNA in ESC, which might further enhance the proliferation of ESC and subsequently result in the formation of ectopic endometrial implants in adenomyosis. (clinicaltrials.gov)
- In vitro effects of benzene metabolites on mouse bone marrow stromal cells. (cdc.gov)
- The effects of benzene (71432) metabolites on bone marrow stromal cells were investigated in-vitro. (cdc.gov)
- The authors conclude that benzene metabolites are toxic to mouse bone marrow stromal cells in-vitro. (cdc.gov)
- Bone marrow adherent cell types, collectively referred to as stromal cells, appear to be key players in such escape, mainly because CLL malignant cells, which rapidly undergo spontaneous apoptosis when cultured in vitro, survive, migrate, and resist cytotoxic agents in co-culture with bone marrow stromal cells. (dovepress.com)
- Interestingly, TSLP preferentially stimulated the proliferation and survival of CD4 + single positive thymocytes and peripheral T cells in vitro. (rupress.org)
- Additionally, CD4 + T cells from TSLPR KO mice expanded less efficiently than WT CD4 + T cells in irradiated hosts, and TSLP preferentially expanded CD4 + T cells both in vitro and in vivo. (rupress.org)
- Finally, carboxyfluorescein succinimidyl ester (CFSE)-based experiments showed TSLP-dependent expansion of CD4 + T cells both in vitro and in vivo. (rupress.org)
- Indeed, secretions from quiescent ECs muted the proliferative and invasive phenotype of lung and breast cancer cells in vitro and reduced cancer cell protumorigenic and proinflammatory signaling. (sciencemag.org)
- From mouse lymph nodes, we established a VCAM-1 + ICAM-1 + MAdCAM-1 + reticular cell line that can produce CXCL13 upon LTβR stimulation and support primary B cell adhesion and migration in vitro. (jimmunol.org)
- In vitro dTc proliferation and tumoricidal capacity in the presence of KIT+ tumor cells were measured. (harvard.edu)
- Human anti-KIT dTc were efficient at lysing GIST in vitro compared to untransduced T cells. (harvard.edu)
- The overall aim of this work was to attempt to induce early angiogenesis in human dental pulp stromal cells (DPSCS) in vitro and in vivo using a biomimetic approach based on combining scaffolds comprised of ECM components with DPSCs as a first step towards a tissue engineering strategy for dental pulp regeneration. (bl.uk)
- We have shown that hypoxia preconditioning provides generalized protection to corneal stromal cells against induced apoptosis in vitro and in an ex vivo cornea model. (molvis.org)
- The term BM stromal cells is also used to describe the adherent cell population established from the in vitro culture of BM, and the predominant cell present in such an adherent cell population is generally the adventitial reticular/fibroblast-like cell. (springer.com)
- Dittel, B. N. and LeBien, T. W. (1995) Reduced expression of vascular cell adhesion molecule-1 on bone marrow stromal cells isolated from marrow transplant recipients correlates with a reduced capacity to support human B lymphopoiesis in vitro. (springer.com)
- ARA55 expression was higher in PZ-old cells compared with PZ-young cells in vitro. (urotoday.com)
- 2-5 In the late 1960s, Friedenstein and colleagues established that single cell suspensions of BM stroma could generate colonies of adherent fibroblast-like cells in vitro. (stemcell.com)
- 7,8 Functional in vitro characterization of the stromal compartment by Dexter et al. (stemcell.com)
- Maturation and stimulation with TSLP or polyriboinosinic-polyribocytidylic acid in vitro upregulated the expression of both TSLPR and IL-7Ralpha chains in DC but not in chemoattractant receptor-homologous molecule expressed on Th2 cells(+) CD4(+) T cells. (unboundmedicine.com)
- Gaede K.I., Mamat U., Schlaak M. et al (1999) Analysis of differentially regulated mRNAs in monocytic cells induced by in vitro stimulation, J. Mol. (scirp.org)
- In contrast, human tumor stromal cells showed little to no evidence of proliferation. (jax.org)
- Abnormal cell proliferation has been generally found in the tumorigenesis, including the formation of endometriosis. (clinicaltrials.gov)
- Adenomyosis is considered to have a similar pathophysiology with endometriosis, and it must be interesting to examine whether there is abnormal cell proliferation in the eutopic endometrium of adenomyosis. (clinicaltrials.gov)
- Therefore, whether abnormal cell proliferation occurs under the effects of LPS and IFN-g in the eutopic endometrium of adenomyosis needs further clarification. (clinicaltrials.gov)
- Estradiol (E2) was demonstrated to induce endometrial cell proliferation, whereas medroxyprogesterone (MPA) inhibited endometrial cell proliferation via antagonizing estrogenic effects. (clinicaltrials.gov)
- Quantification of cell proliferation was done with Cell Proliferation Assay Kit and immunocytochemical detection of Ki-67, in an attempt to examine the cell proliferation of ESCs in women with adenomyosis. (clinicaltrials.gov)
- Quantification of cell proliferation was done with Cell Proliferation Assay Kit and immunocytochemical detection of Ki-67. (clinicaltrials.gov)
- As herein discussed, these molecules probably form a complex network favoring CLL cell survival, proliferation, and chemoresistance to anticancer therapy. (dovepress.com)
- In addition, cell-cell contact involving programmed death-ligand 1 (PD-L1) resulted in inhibition of T-cell proliferation and induction of T regulatory cells ( 2 , 8 ). (frontiersin.org)
- HS5 secretes multiple cytokines that support proliferation of committed progenitor cells, and HS27a supports "cobblestone area" formation by early hematopoietic progenitor cells. (thermofisher.com)
- Genetic deletion of ferroportin in macrophages resulted in iron deficiency and decreased proliferation in epithelial cells, which consequently impaired hair follicle growth and caused transient alopecia. (haematologica.org)
- Iron retention in macrophages had no impact on the inflammatory processes accompanying wound healing, but affected stromal cells proliferation, blood and lymphatic vessels formation, and fibrogenesis. (haematologica.org)
- It is thought that GABA-mediated depolarization results in the influx of Ca 2+ which then regulates progenitor cell proliferation and development. (jneurosci.org)
- SDF-1 also regulates the proliferation of these cells. (jneurosci.org)
- In the co-culture system with PZ-old cells, Pc3/Du145 cells showed advanced proliferation and migration after Dihydrotestosterone (DHT) incubation, but DHT didn't show the similar effect in PZ-young co-culture system. (urotoday.com)
- From our study, we found PZ stromal cells presented age-related effects in proliferation and migration of prostate cancer cells in the androgen/AR dependent manner. (urotoday.com)
- Results: Five days after the addition of EC to MSC in a ratio of 1:5 (EC/MSC) significant increases in cell proliferation and cellular bridges between the two cell types were detected, as well as increased mRNA expression of alkaline phosphatase (ALP). (uib.no)
- Approaches to enhance β-cell mass by increasing proliferation and survival are desirable. (diabetesjournals.org)
- In this condition, a high level of DNA CpG highlands hypermethylation was detected at gene loci involved in cell proliferation including CDK2, CDK4, CDKN1B, CCNB1 and MCM2. (ahajournals.org)
- Barrier cells are recently recognized fibroblastic contractile stromal cells that fuse to form complex branched variform barriers used for such diverse functions as controlling blood flow and blood cell delivery into the circulation, sealing off the hematopoietic/immunologic colonies and regulating their proliferation and differentration through paracrine secretion. (ajtmh.org)
- Rasmussen I., Ringen O., Sundberg B. et al (2005) Mesenchymal stem cells inhibit lymphocyte proliferation by mitogens and alloantigens by different mechanisms, Exp. (scirp.org)
- Sphingosine 1-Phosphate Receptor 1 Is Required for MMP-2 Function in Bone Marrow Mesenchymal Stromal Cells: Implications for Cytoskeleton Assembly and Proliferation. (thefreelibrary.com)
- Indeed, structural and molecular changes in ECM composition and interactions between ECM components and the cell itself can trigger intracellular signalling pathways involved in the control of different cell processes, such as cytoskeletal rearrangement, spreading, survival, proliferation, and migration [15- (thefreelibrary.com)
- Intravenous transplantation of mesenchymal stem cells preconditioned with early phase stroke serum: current evidence and study protocol for a randomized trial. (springer.com)
- Mesenchymal stem cells for treatment of therapy-resistant graft-versus-host disease. (springer.com)
- Plasticity of marrow-derived stem cells. (springer.com)
- To receive news and publication updates for Stem Cells International, enter your email address in the box below. (hindawi.com)
- There are different types of stem cells that have been classified according to their potency. (intechopen.com)
- Multipotent stem cells that is, embryonic cells from the 14th day onward, have the ability to form all the differentiated cell types of a given tissue. (intechopen.com)
- The stem cells that maintain only one lineage are described as unipotent [ 2 ]. (intechopen.com)
- Embryonic stem cells (ESCs) have the greatest potential to differentiate into all cell types. (intechopen.com)
- In the present study we tested a new thymidine analog, 5-ethynyl-2-deoxyuridine (EdU), for labeling and tracking of mesenchymal stromal cells (MSC), specifically adipose tissue-derived stem cells (ADSC). (nih.gov)
- Still, there are considerable challenges before effective stem cell treatment can be realized in emphysema patients. (rug.nl)
- Human primary stromal cells (mesenchymal stem and progenitor cells) are produced by expanding bone marrow mononuclear cells (MNCs) in culture and cryopreserved following the first passage in culture. (stemcell.com)
- Bone-marrow-derived, non-hematopoietic multipotential cells that support Hematopoetic stem cells . (jove.com)
- As stem cells can be grown and transformed into specialized cells with characteristics consistent with cells of various tissues such as muscles or nerves, their use in medical therapies has been proposed. (patientslikeme.com)
- The tissue would be processed to extract the stromal layer of cells that contain stem cells. (mayo.edu)
- They have been widely used clinically to improve the outcome of hematopoietic stem cell and solid organ transplants and to treat graft-vs. -host disease ( 2 , 3 ). (frontiersin.org)
- Bone marrow stromal cells are one of several adult stem cells, and their unique characteristics make them promising candidates for clinical applications. (drexel.edu)
- Hematopoietic stem cells reside in bone marrow and develop into all the types of blood cells in the body. (drexel.edu)
- Other supportive cells in the marrow provide support and nutrition to these stem cells. (drexel.edu)
- Personal cell therapy for interstitial cystitis with autologous stromal vascular fraction stem cells. (urotoday.com)
- The objective of this study was to evaluate whether autologous stem-cell-based therapy may mitigate the symptoms of interstitial cystitis. (urotoday.com)
- Stromal vascular fraction (SVF) rich in stem cells and derived from autologous adipose tissue was deployed into 109 men and women with interstitial cystitis/painful bladder syndrome as a surgical procedure. (urotoday.com)
- This stem-cell-rich biologic product was injected both systemically and regionally into pelvic floor targets. (urotoday.com)
- Correspondence: Jan E. Brinchmann, M.D., Ph.D., Norwegian Center for Stem Cell Research and Institute of Immunology, Oslo University Hospital-Rikshospitalet, P.O. Box 1121 Blindern, 0317 Oslo, Norway. (wiley.com)
- On the other hand, multiple transduction cycles or antibiotic-based selection methods may alter the stem cell phenotype. (ovid.com)
- In contrast, CD14, CD79α and the embryonic stem cell markers Oct-4, SSEA (stage specific embryonic antigen) -1, -3, -4, TRA (tumor rejection antigen) -1-60 and -1-81 are not expressed. (dovepress.com)
- The gene for stem cell factor (SCF), the natural ligand for KIT, was cloned into 1st generation (SCF-CD3ζ, 1st gen) and 2nd generation (SCF-CD28-CD3ζ, 2nd gen) CIR constructs. (harvard.edu)
- Mammalian bone marrow (BM) is a complex milieu of rare pluripotent stem cells, developmentally restricted stem cells, a range of immature to mature cells in distinct lymphohematopoietic lineages, and nonlymphohematopoietic cells (1) . (springer.com)
- Since stem/progenitor cells recruited either from bone marrow or residing in nearby tissues can contribute to pathological processes we investigated endosialin in MSC using a novel monoclonal antibody. (spandidos-publications.com)
- 5 While differentiating from hematopoietic stem cells, megakaryocytes migrate between 2 distinct microenvironments: the endosteal niche and the vascular niche. (bloodjournal.org)
- Osteoblasts are derived from mesenchymal stem cells and the tightly regulated process is controlled by a cascade of transcription factors [ 3 , 4 ]. (portlandpress.com)
- A research study from the Farber Institute for Neurosciences and the Department of Neuroscience at Thomas Jefferson University determines bone marrow stromal stem cells may aid in stroke recovery. (nutritionreview.org)
- The study examining the effects of a systematic administration of either rat (allogenic) or human (xenogenic) bone marrow stem cells (MSC) administered to laboratory rats one day after their simulated strokes found "significant recovery" of motor behavior on the first day. (nutritionreview.org)
- The timing of stem cell treatment was critical to the magnitude of the positive effects," said the study's lead author, Lorraine Iacovitti, Ph.D., professor, Department of Neuroscience at Jefferson Medical College of Thomas Jefferson University. (nutritionreview.org)
- According to Dr. Iacovitti, there has been little research into just how stem cell transplantation modifies inflammatory and immune effects as well as promotes regenerative effects, such as blood vessel growth. (nutritionreview.org)
- The research team concluded that there was "little doubt" that the administration of stem cells can modify the cellular and molecular landscape of the brain and blood, limiting damage and protecting the stroke-injured brain. (nutritionreview.org)
- A major function of the SDF-1/CXCR4 axis is chemoattraction during leukocyte trafficking and stem cell homing, in which local tissue gradients of SDF-1 attract circulating hematopoietic and tissue-committed somatic stem cells ( 3 ). (diabetesjournals.org)
- CXCR4 is expressed in human embryonic stem cells destined to become endoderm ( 8 ). (diabetesjournals.org)
- Stem cells derived from diabetes patients often loose most of their regenerative potential. (ahajournals.org)
- Human mesenchymal stem/stromal cells (hMSCs) are a potential cell source of stem cell therapy for many serious diseases and hMSC spheroids have emerged to replace single cell suspensions for cell therapy. (rsc.org)
- We first demonstrated that poly( N -isopropylacrylamide- co -acrylic acid) (p(NIPAAm-AA)) was not toxic to hMSCs and the microgel-formed constructs facilitated formation of uniform stem cell spheroids. (rsc.org)
- Pevsner-Fisher M., Morad V., Cohen-Sfady M. et al (2007) Toll-like receptors and their ligands control mesenchymal stem cells function, Blood, 109, 1422-1432. (scirp.org)
- Waterman R Waterman RS, Tomchuk SL et al (2010) A New Mesenchymal Stem Cell Paradigm: Polarization into a Pro-Inflammatory MSC-1 or an Immunosupressive VSC-2 Phenotipe, PLOS ONE, 5, 1-14. (scirp.org)
- Connective tissue cells of an organ found in the loose connective tissue. (nih.gov)
- Cell Tissue Kinet. (springer.com)
- They are heterogeneous plastic-adherent cells that are initially expanded from bone marrow (BM) but can be isolated and culture-expanded from adipose tissue, fetal liver, placenta, and umbilical cord blood. (hindawi.com)
- Mesenchymal stromal cell-derived factors promote tissue regeneration of small-for-size livers exposed to ischemic conditions but do not protect against early ischemia and reperfusion injury itself. (mendeley.com)
- Stromal cells are connective tissue cells of any organ, for example in the uterine mucosa (endometrium), prostate, bone marrow, lymph node and the ovary. (wikipedia.org)
- BrdU) has been used widely to label cells in culture and in tissue. (nih.gov)
- Long-term (2-6 week) follow-ups found EdU-positive cells only in the connective tissue. (nih.gov)
- Staining of tissue sections for the human stroma marker vimentin revealed a steady decrease of stromal cell numbers by 4 weeks post-engraftment in the primary host and a total loss of human stroma in secondary recipients. (jax.org)
- Infiltrating stromal and immune cells form the major fraction of normal cells in tumour tissue and not only perturb the tumour signal in molecular studies but also have an important role in cancer biology. (nature.com)
- By performing single-sample gene set-enrichment analysis (ssGSEA) 13 , 23 , we calculate stromal and immune scores to predict the level of infiltrating stromal and immune cells and these form the basis for the ESTIMATE score to infer tumour purity in tumour tissue. (nature.com)
- Non-hematopoietic stromal cells play important roles in many tissues, constructing tissue microenvironments, contributing to tissue repair, defense and immune responses. (keystonesymposia.org)
- M2 macrophages, innate lymphoid type 2 cells (ILC2s), eosinophils, Tregs, and invariant NK T cells (iNKT cells) all help to control adipose tissue inflammation, while M1 macrophages, TNF, and other inflammatory cytokines drive inflammation and insulin resistance in obesity. (jci.org)
- Stromal cells regulate leukocyte responses in lymph nodes, but the role of stromal cells in adipose tissue inflammation is unknown. (jci.org)
- PDGFRα+ stromal cells are major producers of IL-33 in adipose tissue. (jci.org)
- Cadherin-11-deficient mice displayed increased stromal production of IL-33, with concomitant enhancements in ILC2s and M2 macrophages that helped control adipose tissue inflammation. (jci.org)
- D ) Cdh11 mRNA relative to GAPDH in SVF cells and adipocytes from adipose tissue of WT mice ( n = 3) fed a HFD for 5 weeks. (jci.org)
- These cells make up connective tissue that normally supports all tissues and organs both structurally and functionally. (news-medical.net)
- What is Adipose tissue-derived stromal cell transplant? (patientslikeme.com)
- There are no evaluations for Adipose tissue-derived stromal cell transplant. (patientslikeme.com)
- Primary cultures of stromal-vascular (S-V) cells from adipose tissue were used to investigate the regulation of preadipocyte development. (oregonstate.edu)
- S-V cells from both newborn and mature pig adipose tissue and sera from both ages were used to examine the effect of age on preadipocyte development. (oregonstate.edu)
- Cancer cells use a mutant gene to coerce neighbouring healthy tissue into helping with the disease's growth and spread, a major new study reports. (medicalxpress.com)
- in the adult, several distinct stromal lineages construct elaborate tissue architecture and regulate lymphocyte compartmentalization. (jimmunol.org)
- Such tissue geometry is supported by mesenchymal stromal cells, which not only provide a foothold for immune cells' movement and interactions but also have the ability to regulate their homeostasis. (jimmunol.org)
- The reticular network, composed of fibroblastic reticular cells (FRCs) and extracellular matrix (ECM) bundles, is the chief framework supporting the whole tissue architecture ( 2 , 3 , 4 ). (jimmunol.org)
- We have previously demonstrated that transparent membranes produced from fibroin support cultivation of human limbal epithelial cells (Tissue Eng A. 14(2008)1203-11). (arvojournals.org)
- Adhesion of HLS cells to fibroin was initially poorer than that displayed on tissue culture plastic. (arvojournals.org)
- Iron recycling by macrophages is essential for erythropoiesis, but may be also relevant for iron redistribution to neighbouring cells at the local tissue level. (haematologica.org)
- Mesenchymal stromal (MS) cells have been derived from multiple sources in the horse including bone marrow, adipose tissue and umbilical cord blood. (dovepress.com)
- In this chapter, the authors will employ the term BM stromal cell to describe the adventitial reticular/fibroblast-like cell that predominates cultures established from mouse or human BM plated in tissue culture medium supplemented with fetal bovine serum. (springer.com)
- Background: Improved understanding of the interactions between bone cells and endothelial cells involved in osteogenesis should aid the development of new strategies for bone tissue engineering. (uib.no)
- Hydroxyapatite (HA) ceramics together with various kinds of osteogenic cells have been used in bone tissue engineering. (scientific.net)
- 26 Despite their shared markers and perivascular location in vivo, more evidence is required to prove that MSC-like cells in every tissue are derived from or indeed function as pericytes. (stemcell.com)
- 30 Nevertheless, there is no consensus regarding the MSC phenotype, because of the broad variety of potential tissue sources and the differences in cell isolation and cell culture procedures used. (stemcell.com)
- RESULTS- CXCR4 is expressed in β-cells, and SDF-1 is expressed in microvascular endothelial cells within the islets and in surrounding interstitial stromal tissue. (diabetesjournals.org)
- SDF-1 and CXCR4 modulate cell migration and survival during development and tissue remodeling ( 4 , 5 ). (diabetesjournals.org)
- Degree of activation of stromal tissue in immunized mice, apparently, correlates with the degree of immune response, supposing a close relationship between stromal tissue and immune system. (scirp.org)
Marrow Stromal Cells12
- Marrow stromal cells for cellular cardiomyoplasty: feasibility and potential clinical advantages. (springer.com)
- Chen J, Li Y, Wang L, Lu M, Zhang X, Chopp M. Therapeutic benefit of intracerebral transplantation of bone marrow stromal cells after cerebral ischemia in rats. (springer.com)
- M-CSF, LIF), bone marrow stromal cells have been described to be involved in human haematopoiesis and inflammatory processes. (wikipedia.org)
- Human bone marrow stromal cells derived in ACF medium (Catalog #70071) using the MesenCult™-ACF Culture Kit (Catalog #05449) differentiate to A) adipocytes (Oil Red O staining), B) chondrocytes (Alcian Blue and Nuclear Fast Red staining) and C) osteoblasts (Alizarin Red S staining). (stemcell.com)
- Marrow stromal cells provide the possibility for autologous treatment, so a patient may be able to be treated with his or her own cells. (drexel.edu)
- Within the bone marrow, stromal cells help support hematopoietic cells and inflammatory processes. (thermofisher.com)
- Since this role is not understood, the team plans to specifically investigate ITGAV in connection with bone marrow stromal cells. (thermofisher.com)
- Dorshkind, K. (1990) Regulation of hemopoiesis by bone marrow stromal cells and their products. (springer.com)
- Dittel, B. N., McCarthy, J. B., Wayner, E. A., and LeBien, T. W. (1993) Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1). (springer.com)
- Here we report that in human AML, NOX2 generates superoxide, which stimulates bone marrow stromal cells (BMSC) to AML blast transfer of mitochondria through AML-derived tunneling nanotubes. (bloodjournal.org)
- The aim of the present study was to determine whether direct communication between bone marrow stromal cells (MSC) and human umbilical vein endothelial cells (EC) could influence the osteogenic potential of MSC in osteogenic factor-free medium. (uib.no)
- Among the different cell types evaluated to date, bone marrow stromal cells (BMSCs) have received considerable interest due to their potential neuroprotective properties. (wingsforlife.com)
- They also have the ability to create immunomodulatory microenvironment, and thus help to minimize organ damage caused by the inflammation and cells activated by the immune system. (intechopen.com)
- CLL displays variable clinical courses according to well-defined prognostic factors induced on malignant B-cells (CLL cells) or expressed by the transformed bone marrow stromal microenvironment. (dovepress.com)
- CAFs both inhibit immune cell access to the tumor microenvironment and inhibit their functions within the tumor. (springer.com)
- maintain that this proteomic approach is a good starting place for future studies involving molecular mechanisms in the bone marrow microenvironment and crosstalk between stromal cells and myeloma cells in co-culture systems. (thermofisher.com)
- These observations indicate that a reciprocal interaction between CLEC-2 on megakaryocytes and PDPN on BM FRC-like cells contributes to the periarteriolar megakaryopoietic microenvironment in mouse BM. (bloodjournal.org)
- The vascular niche consists of sinusoids and perisinusoidal stromal cells that are present in the maturational microenvironment where platelets are produced and shed into bloodstream. (bloodjournal.org)
- Endothelial cells (ECs) are also ubiquitous within tumors because tumors are vascular, and yet, the impact of tumor-resident ECs is less well understood. (sciencemag.org)
- J. W. Franses, A. B. Baker, V. C. Chitalia, E. R. Edelman, Stromal Endothelial Cells Directly Influence Cancer Progression. (sciencemag.org)
- Endothelial cells can serve as plastic paracrine regulators of cancer biology, modulating tumor growth and metastasis. (sciencemag.org)
- SDF-1 was located in DG neurons and in endothelial cells associated with DG blood vessels. (jneurosci.org)
- We explored the steady-state stromal composition of lymph nodes isolated from mice and humans, and found that marginal reticular cells and lymphatic endothelial cells required lymphocytes for their normal maturation in mice. (harvard.edu)
- Cultured primary endothelial cells express SDF-1, where it is required for the regulation of branching morphogenesis ( 10 ). (diabetesjournals.org)
- However, other reports suggest that endothelial cells display SDF-1 by the transcytosis of SDF-1 produced by perivascular fibroblast-like cells ( 13 , 14 ). (diabetesjournals.org)
- In endothelial cells, ENL inhibited E2-induced VEGFR-2 expression, whereas GEN did not. (diva-portal.org)
- Stromal cells found within epithelial tumors can have a significant impact on the growth, vascularization, invasiveness and metastatic potential of the cancer. (jax.org)
- Immunohistochemical evaluation of tumors grown subcutaneously in NSG and NRG mice demonstrated that human colon cancer epithelial cells remained highly proliferative. (jax.org)
- Immunohistochemistry and direct fluorescence confirmed that mouse-derived stromal cells enter the human colon tumors following re-engraftment into the NSG -GFP secondary hosts. (jax.org)
- Tumors showed disrupted cell organization, decreased cell viability, blood vessel loss, necrosis and mouse leukocyte infiltration. (jax.org)
- In spite of major improvements in clinical management, gastrointestinal stromal tumors (GISTs) can still be deadly due to metastasis and recurrences, which confirms the unmet need of reliable follow-up modalities. (sigmaaldrich.com)
- In addition to a mass of cancer cells, tumors contain several other types of cells known collectively as stromal cells. (news-medical.net)
- The research in this three-year NSF grant will utilize his lab's patented technology to make 3D culture models that mimic the morphology of tumors and reproduce the interactions between stromal and cancer cells. (news-medical.net)
- This research examined how conjugated dietary fatty acids infuence stromal cells to inhibit blood vessel growth in rat mammary tumors. (aicr.org)
- The laser beam can dissect tumors one cell at a time. (cancernetwork.com)
- Immunohistochemistry for human endosialin in xenograft tumors following co-injection of MSC and cancer cells identified MSC in tumor stroma. (spandidos-publications.com)
- T helper 2 (Th2) cells are defined by their ability to produce the hallmark cytokine IL-4. (pnas.org)
- Here we report that a substantial proportion of naive CD4 + T cells in spleen and lymph node express receptors for the epithelium-derived inflammatory cytokine thymic stromal lymphopoietin (TSLP). (pnas.org)
- Thymic stromal lymphopoietin (TSLP) signals via a receptor comprising the interleukin (IL)-7 receptor α chain and a distinctive subunit, TSLP receptor (TSLPR), which is most related to the common cytokine receptor γ chain, γ c . (rupress.org)
- Thymic stromal lymphopoietin (TSLP) is a cytokine that was originally identified as a growth factor in the supernatant of the Z210R.1 thymic stromal cell line that could support the development of immature NAG8/7 B cells to the B220 + /IgM + stage ( 5 ). (rupress.org)
- Conclusions: Results demonstrate that FL is produced spontaneously by BM stromal cells and that FL production is regulated by cytokine stimulation but not by ionizing radiations. (irsn.fr)
- Thymic stromal lymphopoietin (TSLP) is an interleukin (IL)-7-like cytokine that triggers dendritic cell-mediated T helper (Th)2 inflammatory responses through a receptor consisting of a heterodimer of the IL-7 receptor alpha (IL-7Ralpha) chain and the TSLP receptor (TSLPR), which resembles the cytokine receptor common gamma chain. (unboundmedicine.com)
- Gorskaya, U. , Danilova, T. , Mezentseva, M. , Shapoval, I. and Nesterenko, V. (2011) Effect of antigens on colony forming efficiency of stromal clonogenic cells and expression of cytokine genes in primary cultures of bone marrow and spleen of mice. (scirp.org)
- Allergen exposure primes IL-4 + Th2 cells in lymph node, but production of effector cytokines including IL-5 and IL-13 is thought to require additional signals from antigen and the environment. (pnas.org)
- In addition to organizing T and B cell segregation and expressing lymphocyte survival factors, stromal cells support the migration and interactions between antigen presenting cells and naïve T and B cells during the initiation of immune responses and influence the outcome between tolerance and immunity. (keystonesymposia.org)
- Recombinant fragment, corresponding to a region within amino acids 1-146 of Human Bone marrow stromal cell antigen 1. (abcam.com)
- Flow cytometric analysis revealed that pMSC expressed surface antigens also found on hMSC, including CD90, MSCA-1 (TNAP/W8B2 antigen), CD44, CD29 and SLA class I. Clonogenic outgrowth was significantly enriched following selection of CD271+ cells from BM of human and pig (129 ± 29 and 1961 ± 485 fold, respectively). (wiley.com)
- There are currently no images for Bone marrow stromal cell antigen 1/CD157 Antibody (AF4710). (novusbio.com)
- CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). (novusbio.com)
- A gene on chromosome 4p15 that encodes bone marrow stromal cell antigen-1, a glycosylphosphatidylinositol-GPI-anchored molecule that facilitates pre-B-cell growth by synthesising cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. (thefreedictionary.com)
- The majority of TSLPR(+) cells were DC co-expressing blood DC antigen-1 (BDCA-1) or BDCA-2. (unboundmedicine.com)
- Injection of S. typhimurium antigen complex to mice CBA increased 5 times colony forming efficiency (CFE) and, respectively, content of stromal precursor cells (CFU-F) in femur bone marrow and 9 times in spleen of these animals with maximum at the first day. (scirp.org)
- We observed that CXCR4 receptors were expressed by dividing neural progenitor cells located in the subgranular zone (SGZ) as well as their derivatives including doublecortin-expressing neuroblasts and immature granule cells. (jneurosci.org)
- Acute myeloid leukemia (AML) cells have increased mitochondria compared with nonmalignant CD34 + hematopoietic progenitor cells. (bloodjournal.org)
- SDF-1 expressed in bone marrow inhibits the apoptosis of myeloid progenitor cells and promotes their survival ( 6 , 7 ). (diabetesjournals.org)
- We used IL-4 and IL-13 dual-reporter mice to show that naive CD4 + T cells cultured in the presence of IL-4 and thymic stromal lymphopoietin (TSLP) generate a population of IL-4 neg IL-13 pos Th2 cells that develop from IL-4 neg precursors and express the Th2 effector cytokines IL-5 and IL-9. (pnas.org)
- Thymic stromal lymphopoietin (TSLP) is an important factor responsible for the pathogenesis of allergic diseases, such as atopic dermatitis and asthma. (mdpi.com)
- Moon P-D, Han N-R, Lee JS, Kim H-M, Jeong H-J. Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells. (mdpi.com)
- Like IL-7 KO mice, IL-7Rα KO mice exhibit severely reduced thymic cellularity and defective T cell maturation ( 2 , 3 ). (rupress.org)
- In addition, daily injection of TSLP into γ c KO mice increased both thymic and splenic cellularities by enhancing the expansion of both T and B cells, with the accumulation of CD4 + T cells in the periphery. (rupress.org)
- Seeding of thymic microenvironments defined by distinct thymocyte-stromal cell interactions is developmentally controlled. (biomedsearch.com)
Tracking of mesenchymal stromal cells1
- Peritoneal injection of EdU resulted in the appearance of EdU-positive cells in most organs and tissues. (nih.gov)
- In the intestine, EdU-positive cells were found in both the epithelium and connective tissues 7 h after injection. (nih.gov)
- The NSG-GFP hosts express green fluorescent protein in most cells and tissues. (jax.org)
- However, to mediate allergic inflammation in tissues, Th2 cells must secrete additional cytokines including IL-13 and IL-5. (pnas.org)
- Malignant solid tumour tissues consist of not only tumour cells but also tumour-associated normal epithelial and stromal cells, immune cells and vascular cells. (nature.com)
- The comprehensive understanding of tumour-associated normal cells in tumour tissues may provide important insights into tumour biology and aid in the development of robust prognostic and predictive models. (nature.com)
- Here we present a new algorithm that takes advantage of the unique properties of the transcriptional profiles of cancer samples to infer tumour cellularity as well as the different infiltrating normal cells, called ESTIMATE (Estimation of STromal and Immune cells in MAlignant Tumour tissues using Expression data). (nature.com)
- We focus on stromal and immune cells that form the major non-tumour constituents of tumour samples and identify specific signatures related to the infiltration of stromal and immune cells in tumour tissues 1 . (nature.com)
- Stromal cells also play instrumental roles in coordinating immune responses in non-lymphoid tissues, in inflammatory and autoimmune diseases, and in chronic infection. (keystonesymposia.org)
- Mesenchymal stromal cells (MSC) are multipotent cells that can be derived from many different organs and tissues. (semanticscholar.org)
- Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. (jove.com)
- The identification of such profiles in the horse will allow the comparison of putative MS cells isolated from different laboratories and different tissues. (dovepress.com)
- Stromal cell-derived factor (SDF)-1/CXCL12 is a peptide chemokine initially identified in bone marrow-derived stromal cells and now recognized to be expressed in stromal tissues in multiple organs ( 1 - 3 ). (diabetesjournals.org)
- Malignant cancer cells also express CXCR4, and their survival and migration to distant tissues is promoted by SDF-1 ( 9 ). (diabetesjournals.org)
- Therefore, the ideal strategy should target not only T cells, which are the main players of alloimmunity, but regulate in a concerted action also B cells, dendritic cells and macrophages, which all contribute to both the acute and chronic alloimmune response. (frontiersin.org)
- The results identify associations of thymocytes with I-A- macrophages in the cortex as the earliest discernible cell-cell interactions during thymopoiesis. (biomedsearch.com)
Mesenchymal Stromal Cell-Derived1
- The remaining ADSCs showed a partial dependence on dendritic cells (DCs) for survival. (jci.org)
- B cells form follicles (B zone) and occasionally develop germinal centers during antigenic stimulation, while the majority of T cells accumulate adjacent to the follicles (T zone) and survey cognate Ags presented by dendritic cells (DCs). (jimmunol.org)
- Two distinct stromal cell types, residing in different compartments and producing specific chemokines, play key roles in the localization of lymphocytes: in the B zone, follicular dendritic cells (FDCs) expressing CXCL13 and in the T zone, FRCs expressing CCL19 and CCL21 ( 7 , 8 , 9 ). (jimmunol.org)
- After a significant delay, this recognition stage is followed by concomitant interactions of T cells with I-A+ epithelial cells in the cortex and bone marrow-derived I-A+ dendritic cells in the medulla. (biomedsearch.com)
- Dendritic cells activated by TSLP prime development of CD4(+) T cells into Th2 cells contributing to the pathogenesis of allergic inflammation. (unboundmedicine.com)
- Minimal criteria for defining multipotent mesenchymal stromal cells. (semanticscholar.org)
- As mechanisms underlying management of reactive oxygen species (ROS) gain interest as crucial factors in cell integrity, questions arise about the role of redox cues in regulation and maintenance of bone marrow-derived multipotent stromal cells (BMSCs) that contribute to wound healing, particularly in diabetes. (diabetesjournals.org)
- Normalized Nrf2/Keap1 signaling restores multipotent cell properties in diabetic BMSCs through Sox2 expression. (diabetesjournals.org)
- Mesenchymal stromal cells are excellent candidates for regenerative medicine since they are multipotent, easy to isolate, can be expanded to obtain clinically relevant numbers and are immunoprivileged. (ovid.com)
- Osteoblasts, the bone-forming cells, derive from multipotential bone marrow stromal precursors called colony-forming units-fibroblastic (CFU-F). CFU-F rapidly adhere to plastic upon culture ex vivo, adhesion of such stromal precursors to bone in vivo is likely to be an early event in the anabolic response to bone stimulatory factors. (bl.uk)
- Reticular fibroblastic and vascular stromal cells, important for secondary lymphoid organ formation and organization, express RANK and undergo hyperproliferation, which is abrogated by RANKL neutralization. (jimmunol.org)
- Although devoid of direct effects on cells of hematopoietic origin, hIL-17 and the product of its viral counterpart, ORF13, stimulate epithelial, endothelial, and fibroblastic cells to secrete cytokines such as IL-6, IL-8, and granulocyte-colony-stimulating factor, as well as prostaglandin E2. (rupress.org)
- We have also identified PDPN-expressing BM arteriolar stromal cells, tentatively termed as BM fibroblastic reticular cell (FRC)-like cells. (bloodjournal.org)
- We also report alterations in the proportion and number of fibroblastic reticular cells (FRCs) between skin-draining and mesenteric lymph nodes. (harvard.edu)
Types of stromal cells1
Role of stromal cells1
- Hossein Tavana, Ph.D., an associate professor of biomedical engineering at The University of Akron (UA), has received a single-PI grant in the amount of $328,426 from the National Science Foundation (NSF) - his third federal grant this year - to study the role of stromal cells in cancer. (news-medical.net)
Epithelial and stromal cells1
Results suggest that stromal1
Endometrial stromal cell1
- Purpose: To define the ability of human bone marrow (BM) stromal cells to produce Flt3 ligand (FL), and to define the effect of ionizing radiation, TNF or TGF on FL production. (irsn.fr)
- Jarvis, L. J. and LeBien, T. W. (1995) Stimulation of human bone marrow stromal cell tyrosine kinases and IL-6 production by contact with B lymphocytes. (springer.com)
- 1 CFU-F initiating cells in vivo have been shown to be quiescent, existing at a low frequency in human bone marrow. (stemcell.com)
- Neuronal plasticity of human Wharton's jelly mesenchymal stromal cells to the dopaminergic cell type compared with human bone marrow mesenchymal st. (nih.gov)
- Human umbilical vein endothelial cell (HUVEC) tube formation and fibrin gel bead assay (FIBA) sprout formation were used to assess the angiogenic properties of Co-MSC secretome. (arvojournals.org)
- Mesenchymal stromal cells (MSC) derived from Wharton's jelly (WJ) of the umbilical cord are increasingly gaining prominence as substitutes for bone marrow (BM) MSC. (nih.gov)
- In vivo, high TSLP levels promote the development of a similar population of IL-4 neg IL-13 pos T cells that also express Gata3 , Il5 , and Il3 transcripts. (pnas.org)
- In vivo, high TSLP levels acted directly on CD4 + T cells to induce the development of IL-13-SP and IL-4 + IL-13 + double-positive populations in lymph node. (pnas.org)
- C ) Confocal microscopic images of cadherin-11 expression (green) at adherens junctions on day-2 ex vivo SVF cell cultures. (jci.org)
- Reversion of the malignant phenotype of human breast cells in three-dimensional culture and in vivo by integrin blocking antibodies. (springer.com)
- In vivo, the consistent results were also found: PZ-old cells promoted prostate cancer cells growth, but this effect receded when knocking down ARA55 expression in PZ-old cells. (urotoday.com)
- The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. (springer.com)
- Here, we show that mesenchymal cadherin-11 modulates stromal fibroblast function. (jci.org)
- Le Hir M, Hegyi I, Cueni-Loffing D, Loffing J, Kaissling B. Characterization of renal interstitial fibroblast-specific protein 1/S100A4-positive cells in healthy and inflamed rodent kidneys. (springer.com)
- We now report that human colonic fibroblast cell lines produce significant amounts of PGI 2 and that fibroblast lines derived from normal-appearing colonic mucosa of hereditary nonpolyposis CRC individuals produce 50-fold more PGI 2 than normal fibroblast lines derived from individuals with nonhereditary CRC. (aacrjournals.org)
- The present studies were performed to characterize PG production by human pericryptal fibroblast cell lines and to test the hypothesis that stromal PGs affect epithelial function and contribute to colon carcinogenesis. (aacrjournals.org)
- HLS cultures containing cells of predominantly fibroblast lineage can be grown on fibroin-based materials, but this process is dependent upon additional ECM factors such as those provided by serum. (arvojournals.org)
- Whether adventitial reticular cells constitute a single cell type with origin in a common mesenchymal precursor is not known, but they manifest many attributes of fibroblast-like cells (3) . (springer.com)
- Stroma is made up of the non-malignant cells, but can provide an extracellular matrix on which tumor cells can grow. (wikipedia.org)
- Researchers now recognize that therapies developed using these homogeneous cell lines, which are devoid of stroma, translate poorly to the clinic. (jax.org)
- The overall maintenance of tumor growth and structural architecture suggested that the human stromal cells were dynamically replaced by mouse-derived stroma. (jax.org)
- Studies of the interactions between tumour cells and the tumour stroma in regulating tumour metastasis have been the subject of intense investigations. (nature.com)
- A dynamic and mutualistic interaction between tumour cells and the surrounding stroma promotes the initiation, progression, metastasis and chemoresistance of solid tumours. (nih.gov)
- Angiogenesis is a key in cancer progression and its regulators are released both by the tumor cells and the stroma. (diva-portal.org)
- In this study, human breast cancer cells (MCF-7) were established in mice creating a tumor with species-specific cancer and stroma cells. (diva-portal.org)
- ENL and ENL+GEN decreased both stroma- and cancer cell-derived VEGF, whereas cancer cell-derived PlGF increased. (diva-portal.org)
- These results suggest that ENL has potent effects on breast cancer growth, even in combination with GEN, by downregulating E2-stimulated angiogenic factors derived both from the stroma and the cancer cells, whereas dietary GEN does not possess any antiestrogenic effects. (diva-portal.org)
Expression in stromal1
- However, the transcription and translation of VEGF were paradoxically increased by siHIF-1α, suggesting that VEGF expression in stromal cells is not down-stream of HIF-1α. (molvis.org)
- These immunomodulatory activities are mediated by both cell-cell interactions and secreted cytokines including interferon- (IFN-) γ , indoleamine 2,3-dioxygenase (IDO), transforming growth factor- (TGF-) β , interleukin (IL-) 6, IL-10, and prostaglandin E2 [ 20 - 23 ]. (hindawi.com)
- A major effort of this project will be testing rationally-selected combinations of drugs that block interactions of stromal and cancer cells, prevent growth of cancer cells and maintain their sensitivity to drugs. (news-medical.net)
- Characterizing the sets of signaling pathways involved in the interactions between stromal cells and CLL cells may provide new tools for CLL clinical phenotyping and for re-sensitizing chemotherapy resistant cells. (dovepress.com)
- Seeding of distinct intrathymic microenvironments defined by direct thymocyte-stromal cell interactions was correlated with T cell development in situ using radiation and nonradiation chimeras of Thy-1.1/1.2 congenic mice. (biomedsearch.com)
- All three types of T cell-stromal cell interactions occur after seeding of the intrathymic precursor cell subset and before development of mature medullary-type T cells. (biomedsearch.com)
- The seeding kinetics imply that recognition of cortical epithelial cells by thymocytes in situ represents a relatively late stage of cortical T cell development, whereas thymocyte-dendritic cell interactions denote a very early stage of T cell development in the medulla. (biomedsearch.com)
- BM FRC-like cells regulate megakaryocytic clonal expansion via CLEC-2/PDPN interactions. (bloodjournal.org)
Bone marrow-derived mesenchymal1
- Cellular therapy with mesenchymal stromal cells (MSC) has recently emerged as a promising strategy to regulate anti-donor immune responses, allowing immunosuppressive drug minimization and tolerance induction. (frontiersin.org)
- The therapeutic effect of ADSCs has been attributed to its secretion of paracrine factors, which can regulate the metabolism of target cells. (aacrjournals.org)
- The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (google.com)
- Bone marrow cell suspensions were prepared from male B6C3F1-mice and cultured with hydroquinone (123319), benzoquinone (106514), catechol (120809), phenol (108952), and 1,2,4-benzenetriol (533733) at concentrations from 0.0000015 to formed in the presence of these metabolites was determined and compared with values from untreated stromal cell cultures. (cdc.gov)
- Marrow samples from 89 patients with aplastic anemia (AA) were evaluated for their ability to grow stromal layers in standard long- term marrow cultures (LTMCs). (bloodjournal.org)
- Primary cultures of HLS cells were established in DMEM/F12 medium supplemented with either 10% fetal bovine serum (FBS) or 2% B27 supplement. (arvojournals.org)
- The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. (wikipedia.org)
- Stromal cells (in the dermis layer) adjacent to the epidermis (the very top layer of the skin) release growth factors that promote cell division. (wikipedia.org)
- The loss of these stromal growth factors when the cancer moves throughout the body prevents the cancer from invading other organs. (wikipedia.org)
- 17 Beside its role in supporting tumour growth and angiogenesis, CXCL12 was demonstrated to be important in helping select metastatic tumour cells for bone metastasis. (nature.com)
- Stromal cells exposed to metabolites at these same concentrations were also cultured simultaneously with fresh bone marrow cells in agar to measure the effect on the ability of stromal cells to support growth of granulocyte monocyte colony forming cells. (cdc.gov)
- Hydroquinone completely inhibited cell growth at concentrations above 0.0000125mole, and benzoquinone had the same effect at a concentration of 0.00005mol. (cdc.gov)
- Research in recent years has shown that stromal cells play a major role in cancer growth and progression. (news-medical.net)
- Treatments have only focused on the cancerous cells and largely neglected the stromal cells that contribute to tumor growth and persistence despite treatments. (news-medical.net)
- May be involved in pre-B-cell growth. (abcam.com)
- Ontologies such as peptidases, cell adhesion, cell death/cell cycle, growth factors, cytoskeletal organization, defense/immune system, signal transduction, and transcriptional regulation which are related to the development of endometriosis were represented by these genes. (scirp.org)
- Indeed, solid tumor cells' growth and expansion can influence neighboring cells' behavior, leading to a modulation of mesenchymal stromal cell (MSC) activities and remodeling of extracellular matrix components. (mdpi.com)
- Furthermore, MSC can support the survival and growth of leukemic cells within bone marrow participating in the leukemic cell niche. (mdpi.com)
- S-V cells from newborn pigs replicated faster and appeared more responsive to serum borne factors influencing S-V cell growth and development in culture. (oregonstate.edu)
- CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). (novusbio.com)
- These cells form a uniform polarizing monolayer when cultured on Transwell filters, and the epidermal growth factor receptor is found predominantly at the basolateral surface, as it is in all polarized epithelial cells. (aacrjournals.org)
- Basolateral but not apical delivery of the epidermal growth factor receptor ligand transforming growth factor α results in up-regulation of COX-2 and production of PGs that are released exclusively into the basolateral medium of polarized HCA-7 cells. (aacrjournals.org)
- We presently extend this body of work to studies of human limbal stromal cell (HLS) growth on fibroin in the presence and absence of serum. (arvojournals.org)
- However, protection of cortical neurons [ 15 , 16 ], pancreatic cancer cells [ 16 ], and retinal photoreceptors require HIF-1α, which is generally associated with upregulation of protective growth factors such as VEGF (vascular endothelial growth factor) and EPO (erythropoietin). (molvis.org)
- Dittel, B. N. and LeBien, T. W. (1995) The growth response to Il-7 during human B cell ontogeny is restricted to B-lineage cells expressing CD34. (springer.com)
- Stromal cells in the peripheral zone (PZ) of the prostate from older males (PZ-old) could significantly promote Prostate cancer (PCa) growth compared with stromal cells from young males (PZ-young). (urotoday.com)
- After knocking down ARA55 expression in PZ-old cells, the PCa growth- promoting effect from the PZ-old cells was diminished, which may be explained by the decreased the progressive cytokines secretion (FGF-2, KGF, IGF-1) from PZ-old stromal cells. (urotoday.com)
- An understanding of the factors that control β-cell growth and survival could provide new rational approaches for the treatment of diabetes. (diabetesjournals.org)
- The major sources of S1P are represented by activated platelets and by cells subjected to a various degree of damage or stimulation by different growth factors, thus suggesting a potential role for the sphingolipid in many physiological and pathological conditions [17, 25- (thefreelibrary.com)
- Here we describe 'Estimation of STromal and Immune cells in MAlignant Tumours using Expression data' (ESTIMATE)-a method that uses gene expression signatures to infer the fraction of stromal and immune cells in tumour samples. (nature.com)
- A higher percentage of immune cells was found in the white pulp of mice on day 16 of infection, suggesting a role for these cells in the development of crisis. (ajtmh.org)
- Mesenchymal stromal cell transplantation in amyotrophic lateral sclerosis: a long-term safety study. (springer.com)
- Menasche P. Cell transplantation for the treatment of heart failure. (springer.com)
- We will also provide an overview of available data on safety and feasibility of MSC therapy in solid organ transplant patients, highlighting the issues that still need to be addressed before establishing MSC as a safe and effective tolerogenic cell therapy in transplantation. (frontiersin.org)
- In this scenario, mesenchymal stromal cells (MSC) seem a very promising cellular therapy in the pursuit of transplantation tolerance induction, allowing minimization or even discontinuation of life-long immunosuppression. (frontiersin.org)
- Limited functional effects of subacute syngeneic bone marrow stromal cell transplantation after rat spinal cord contusion injury. (wingsforlife.com)
- Cell transplantation might be one means to improve motor, sensory or autonomic recovery after traumatic spinal cord injury (SCI). (wingsforlife.com)
- The results can be found in Cell Transplantation - The Regenerative Medicine Journal. (nutritionreview.org)
- These features make stromal cell populations potential therapeutic targets. (jax.org)
- Our understanding of stromal cell populations and their contributions to innate and adaptive immunity as well as immunological diseases, cancer and vaccination has grown exponentially over the past few years. (keystonesymposia.org)
- Sorting experiments revealed that IL-13-SP Th2 cells originated from IL-4-negative precursors and coexpressed transcripts for the Th2 cytokines IL-5 and IL-9. (pnas.org)
- Chaylakyan R.K., Gerasimov Yu.V., Kuralesova A.I. et al (2001) Izvestiya АS, Proliferative and differential potentions of individual clones of stromal cell precursors in bone marrow, 6, 682- 687. (scirp.org)