A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
An actinomycete from which the antibiotics STREPTOMYCIN, grisein, and CANDICIDIN are obtained.
A soil-dwelling actinomycete with a complex lifecycle involving mycelial growth and spore formation. It is involved in the production of a number of medically important ANTIBIOTICS.
An actinomycete used for production of commercial ANTIBIOTICS and as a host for gene cloning.
An actinomycete from which the antibiotic OLEANDOMYCIN is obtained.
An actinomycete from which the antibiotic CHLORTETRACYCLINE is obtained.
Compounds based on ANTHRACENES which contain two KETONES in any position. Substitutions can be in any position except on the ketone groups.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins found in any species of bacterium.
The functional hereditary units of BACTERIA.
Substances that reduce the growth or reproduction of BACTERIA.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Polyacenes with four ortho-fused benzene rings in a straight linear arrangement. This group is best known for the subclass called TETRACYCLINES.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
4-Methoxy-5-((5-methyl-4-pentyl-2H-pyrrol-2-ylidene)methyl)- 2,2'-bi-1H-pyrrole. A toxic, bright red tripyrrole pigment from Serratia marcescens and others. It has antibacterial, anticoccidial, antimalarial, and antifungal activities, but is used mainly as a biochemical tool.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
A family of soil bacteria. It also includes some parasitic forms.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A group of often glycosylated macrocyclic compounds formed by chain extension of multiple PROPIONATES cyclized into a large (typically 12, 14, or 16)-membered lactone. Macrolides belong to the POLYKETIDES class of natural products, and many members exhibit ANTIBIOTIC properties.
Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Macrolide antibiotic obtained from cultures of Streptomyces fradiae. The drug is effective against many microorganisms in animals but not in humans.
Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
An antibiotic complex produced by Streptomyces kitasatoensis. The complex consists of a mixture of at least eight biologically active components, A1 and A3 to A9. Leucomycins have both antibacterial and antimycoplasmal activities.
Naturally occurring family of beta-lactam cephalosporin-type antibiotics having a 7-methoxy group and possessing marked resistance to the action of beta-lactamases from gram-positive and gram-negative organisms.
Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Mixture of antifungal heptaene macrolides from Streptomyces griseus or Actinomyces levoris used topically in candidiasis. The antibiotic complex is composed of candicidins A, B, C, and D, of which D is the major component.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Naphthalene rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.
Sets of enzymatic reactions occurring in organisms and that form biochemicals by making new covalent bonds.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Class of BACTERIA with diverse morphological properties. Strains of Actinobacteria show greater than 80% 16S rDNA/rRNA sequence similarity among each other and also the presence of certain signature nucleotides. (Stackebrandt E. et al, Int. J. Syst. Bacteriol. (1997) 47:479-491)
The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.
Large enzyme complexes composed of a number of component enzymes that are found in STREPTOMYCES which biosynthesize MACROLIDES and other polyketides.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An order of gram-positive, primarily aerobic BACTERIA that tend to form branching filaments.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
A macrolide antibiotic produced by Streptomyces ambofaciens. The drug is effective against gram-positive aerobic pathogens, N. gonorrhoeae, and staphylococci. It is used to treat infections caused by bacteria and Toxoplasma gondii.
The relationships of groups of organisms as reflected by their genetic makeup.
Hydrocarbons with more than one double bond. They are a reduced form of POLYYNES.
A complex of several closely related glycosidic antibiotics from Streptomyces griseus. The major component, CHROMOMYCIN A3, is used as a fluorescent stain of DNA where it attaches and inhibits RNA synthesis. It is also used as an antineoplastic agent, especially for solid tumors.
Antibiotic macrolide produced by Streptomyces antibioticus.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
A mixture of mostly avermectin H2B1a (RN 71827-03-7) with some avermectin H2B1b (RN 70209-81-3), which are macrolides from STREPTOMYCES avermitilis. It binds glutamate-gated chloride channel to cause increased permeability and hyperpolarization of nerve and muscle cells. It also interacts with other CHLORIDE CHANNELS. It is a broad spectrum antiparasitic that is active against microfilariae of ONCHOCERCA VOLVULUS but not the adult form.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
One of the CYCLIC PEPTIDES from Streptomyces that is active against gram-positive bacteria. In veterinary medicine, it has been used in mastitis caused by gram-negative organisms and in dermatologic disorders.
Cyclic esters of hydroxy carboxylic acids, containing a 1-oxacycloalkan-2-one structure. Large cyclic lactones of over a dozen atoms are MACROLIDES.
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
A group of antibiotic aminoglycosides differing only in the number of repeating residues in the peptide side chain. They are produced by Streptomyces and Actinomyces and may have broad spectrum antimicrobial and some antiviral properties.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.
Clavulanic acid and its salts and esters. The acid is a suicide inhibitor of bacterial beta-lactamase enzymes from Streptomyces clavuligerus. Administered alone, it has only weak antibacterial activity against most organisms, but given in combination with other beta-lactam antibiotics it prevents antibiotic inactivation by microbial lactamase.
A TETRACYCLINE analog isolated from the actinomycete STREPTOMYCES rimosus and used in a wide variety of clinical conditions.
Cyclic AMIDES formed from aminocarboxylic acids by the elimination of water. Lactims are the enol forms of lactams.

Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov. (1/4730)

A polyphasic taxonomic study was undertaken to clarify relationships within and between representative thermophilic alkalitolerant streptomycetes isolated from soil and appropriate marker strains. The resultant data, notably those from DNA-DNA relatedness studies, support the taxonomic integrity of the validly described species Streptomyces thermodiastaticus, Streptomyces thermoviolaceus and Streptomyces thermovulgaris. However, the genotypic and phenotypic data clearly show that Streptomyces thermonitrificans Desai and Dhala 1967 and S. thermovulgaris (Henssen 1957) Goodfellow et al. 1987 represent a single species. On the basis of priority, S. thermonitrificans is a later subjective synonym of S. thermovulgaris. Similarly, 10 out of the 11 representative thermophilic alkalitolerant isolates had a combination of properties consistent with their classification as S. thermovulgaris. The remaining thermophilic alkalitolerant isolate, Streptomyces strain TA56, merited species status. The name Streptomyces thermoalcalitolerans sp. nov. is proposed for this strain. A neutrophilic thermophilic isolate, Streptomyces strain NAR85, was identified as S. thermodiastaticus.  (+info)

Diperamycin, a new antimicrobial antibiotic produced by Streptomyces griseoaurantiacus MK393-AF2. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities. (2/4730)

Antibacterial antibiotics, diperamycin (1) was produced in the culture broth of Streptomyces griseoaurantiacus MK393-AF2. Various spectroscopic analyses of 1 suggested that 1 belonged to a member of cyclic hexadepsipeptide antibiotic. Antibiotic 1 had potent inhibitory activity against various Gram-positive bacteria including Enterococcus seriolicida and methicillin-resistant Staphylococcus aureus.  (+info)

Characterization of an insertion sequence element associated with genetically diverse plant pathogenic Streptomyces spp. (3/4730)

Streptomycetes are common soil inhabitants, yet few described species are plant pathogens. While the pathogenicity mechanisms remain unclear, previous work identified a gene, nec1, which encodes a putative pathogenicity or virulence factor. nec1 and a neighboring transposase pseudogene, ORFtnp, are conserved among unrelated plant pathogens and absent from nonpathogens. The atypical GC content of nec1 suggests that it was acquired through horizontal transfer events. Our investigation of the genetic organization of regions adjacent to the 3' end of nec1 in Streptomyces scabies 84.34 identified a new insertion sequence (IS) element, IS1629, with homology to other IS elements from prokaryotic animal pathogens. IS1629 is 1,462 bp with 26-bp terminal inverted repeats and encodes a putative 431-amino-acid (aa) transposase. Transposition of IS1629 generates a 10-bp target site duplication. A 77-nucleotide (nt) sequence encompassing the start codon and upstream region of the transposase was identified which could function in the posttranscritpional regulation of transposase synthesis. A functional copy of IS1629 from S. turgidiscabies 94.09 (Hi-C-13) was selected in the transposon trap pCZA126, through its insertion into the lambda cI857 repressor. IS1629 is present in multiple copies in some S. scabies strains and is present in all S. acidiscabies and S. turgidiscabies strains examined. A second copy of IS1629 was identified between ORFtnp and nec1 in S. acidiscabies strains. The diversity of IS1629 hybridization profiles was greatest within S. scabies. IS1629 was absent from the 27 nonpathogenic Streptomyces strains tested. The genetic organization and nucleotide sequence of the nec1-IS1629 region was conserved and identical among representatives of S. acidiscabies and S. turgidiscabies. These findings support our current model for the unidirectional transfer of the ORFtnp-nec1-IS1629 locus from IS1629-containing S. scabies (type II) to S. acidiscabies and S. turgidiscabies.  (+info)

Electron microscopy studies of cell-wall-anchored cellulose (Avicel)-binding protein (AbpS) from Streptomyces reticuli. (4/4730)

Streptomyces reticuli produces a 35-kDa cellulose (Avicel)-binding protein (AbpS) which interacts strongly with crystalline cellulose but not with soluble types of cellulose. Antibodies that were highly specific for the NH2-terminal part of AbpS were isolated by using truncated AbpS proteins that differed in the length of the NH2 terminus. Using these antibodies for immunolabelling and investigations in which fluorescence, transmission electron, or immunofield scanning electron microscopy was used showed that the NH2 terminus of AbpS protrudes from the murein layer of S. reticuli. Additionally, inspection of ultrathin sections of the cell wall, as well as biochemical experiments performed with isolated murein, revealed that AbpS is tightly and very likely covalently linked to the polyglucane layer. As AbpS has also been found to be associated with protoplasts, we predicted that a COOH-terminal stretch consisting of 17 hydrophobic amino acids anchors the protein to the membrane. Different amounts of AbpS homologues of several Streptomyces strains were synthesized.  (+info)

Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel "unnatural" natural products. (5/4730)

The structures of complex polyketide natural products, such as erythromycin, are programmed by multifunctional polyketide synthases (PKSs) that contain modular arrangements of functional domains. The colinearity between the activities of modular PKS domains and structure of the polyketide product portends the generation of novel organic compounds-"unnatural" natural products-by genetic manipulation. We have engineered the erythromycin polyketide synthase genes to effect combinatorial alterations of catalytic activities in the biosynthetic pathway, generating a library of >50 macrolides that would be impractical to produce by chemical methods. The library includes examples of analogs with one, two, and three altered carbon centers of the polyketide products. The manipulation of multiple biosynthetic steps in a PKS is an important milestone toward the goal of producing large libraries of unnatural natural products for biological and pharmaceutical applications.  (+info)

Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage. (6/4730)

We report DNA and predicted protein sequence similarities, implying homology, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria. The sequence matches reported here establish genetic connections, not always direct, among the lambdoid phages of Escherichia coli, phage phiC31 of Streptomyces, phages of Mycobacterium, a previously unrecognized cryptic prophage, phiflu, in the Haemophilus influenzae genome, and two small prophage-like elements, phiRv1 and phiRv2, in the genome of Mycobacterium tuberculosis. The results imply that these phage genes, and very possibly all of the dsDNA tailed phages, share common ancestry. We propose a model for the genetic structure and dynamics of the global phage population in which all dsDNA phage genomes are mosaics with access, by horizontal exchange, to a large common genetic pool but in which access to the gene pool is not uniform for all phage.  (+info)

Characterization of aklavinone-11-hydroxylase from Streptomyces purpurascens. (7/4730)

Aklavinone-11-hydroxylase (RdmE) is a FAD monooxygenase participating in the biosynthesis of daunorubicin, doxorubicin and rhodomycins. The rdmE gene encodes an enzyme of 535 amino acids. The sequence of the Streptomyces purpurascens enzyme is similar to other Streptomyces aromatic polyketide hydroxylases. We overexpressed the gene in Streptomyces lividans and purified aklavinone-11-hydroxylase to apparent homogeneity with four chromatographic steps utilizing a kinetic photometric enzyme assay. The enzyme is active as the monomer with a molecular mass of 60 kDa; it hydroxylates aklavinone and other anthracyclinones. Aklavinone-11-hydroxylase can use both NADH and NADPH as coenzyme but it is slowly inactivated in the presence of NADH. The apparent Km for NADPH is 2 mM and for aklavinone 10 microM. The enzyme is inactivated in the presence of phenylglyoxal and 2,3-butanedione. NADPH protects against inactivation of aklavinone-11-hydroxylase by phenylglyoxal.  (+info)

A 20-kDa domain is required for phosphatidic acid-induced allosteric activation of phospholipase D from Streptomyces chromofuscus. (8/4730)

Two phospholipase D (PLD) enzymes with both hydrolase and transferase activities were isolated from Streptomyces chromofuscus. There were substantial differences in the kinetic properties of the two PLD enzymes towards monomeric, micellar, and vesicle substrates. The most striking difference was that the higher molecular weight enzyme (PLD57 approximately 57 kDa) could be activated allosterically with a low mole fraction of phosphatidic acid (PA) incorporated into a PC bilayer (Geng et al., J. Biol. Chem. 273 (1998) 12195-12202). PLD42/20, a tightly associated complex of two peptides, one of 42 kDa and the other 20 kDa, had a 4-6-fold higher Vmax toward PC substrates than PLD57 and was not activated by PA. N-Terminal sequencing of both enzymes indicated that both components of PLD42/20 were cleavage products of PLD57. The larger component included the N-terminal segment of PLD57 and contained the active site. The N-terminus of the smaller peptide corresponded to the C-terminal region of PLD57; this peptide had no PLD activity by itself. Increasing the pH of PLD42/20 to 8.9, followed by chromatography of PLD42/20 on a HiTrap Q column at pH 8.5 separated the 42- and 20-kDa proteins. The 42-kDa complex had about the same specific activity with or without the 20-kDa fragment. The lack of PA activation for the 42-kDa protein and for PLD42/20 indicates that an intact C-terminal region of PLD57 is necessary for activation by PA. Furthermore, the mechanism for transmission of the allosteric signal requires an intact PLD57.  (+info)

en] The activity of the D-alanyl-D carboxypeptidase from the penicillin-resistant Streptomyces albus G is not or very little affected by penicillins and related antibiotics. The molecular basis for the mechanism of action of penicillin is discussed. The Streptomyces albus G D-alanyl-D carboxypeptidase appears as a model for the study of a mechanism of penicillin resistance that does not involve the enzymatic degradation of the antibiotic. Vancomycin and ristocetin are shown to inhibit the hydrolysis of sensitive peptides by the Streptomyces albus G D-alanyl-D carboxypeptidase and the mechanism of inhibition is discussed ...
Streptomyces thermoviolaceus ChiS protein: similar to histidine kinases; a member of a two-component sensor-regulator system; isolated from Streptomyces thermoviolaceus; amino acid sequence in first source; GenBank AB016841
Streptomyces isolates have yielded the majority of human, animal, and agricultural antibiotics, as well as a number of fundamental chemotherapy medicines. Streptomyces is the largest antibiotic-producing genus of actinobacteria, producing chemotherapy, antibacterial, antifungal, antiparasitic drugs, and immunosuppressants. Streptomyces isolates are typically initiated with the aerial hyphal formation from the mycelium. Streptomyces, yielded the medicines doxorubicin (Doxil), daunorubicin (DaunoXome), and streptozotocin (Zanosar). Doxorubicin is the precursor to valrubicin (Valstar), myocet, and pirarubicin. Daunorubicin is the precursor to idarubicin (Idamycin), epirubicin (Ellence), and zorubicin. Streptomyces is the original source of dactinomycin (Cosmegen), bleomycin (Blenoxane), pingyangmycin (Bleomycin A5), mitomycin C (Mutamycin), rebeccamycin, staurosporine (precursor to stauprimide and midostaurin), neothramycin, aclarubicin, tomaymycin, sibiromycin, and mazethramycin. Derivatives of ...
P,Following translocation, bacterial lipoproteins are lipidated by lipoprotein diacylglycerol transferase (Lgt) and cleaved of their signal peptides by lipoprotein signal peptidase (Lsp). In Gram-negative bacteria and mycobacteria, lipoproteins are further lipidated by lipoprotein N-acyl transferase (Lnt), to give triacylated lipoproteins. Streptomyces are unusual amongst Gram-positive bacteria because they export large numbers of lipoproteins via the twin arginine protein transport (Tat) pathway. Furthermore, some Streptomyces species encode two Lgt homologues and all Streptomyces species encode two homologues of Lnt. Here we characterize lipoprotein biogenesis in the plant pathogen Streptomyces scabies and report that lgt and lsp mutants are defective in growth and development while only moderately affected in virulence. Lipoproteins are lost from the membrane in an S. scabies lgt mutant but restored by expression of Streptomyces coelicolor lgt1 or lgt2 confirming that both encode functional ...
TY - JOUR. T1 - Efficient Nε-lauroyl-l-lysine production by recombinant ε-lysine acylase from Streptomyces mobaraensis. AU - Koreishi, Mayuko. AU - Kawasaki, Ryoko. AU - Imanaka, Hiroyuki. AU - Imamura, Koreyoshi. AU - Takakura, Yasuaki. AU - Nakanishi, Kazuhiro. PY - 2009/5/20. Y1 - 2009/5/20. N2 - ε-Lysine acylase from Streptomyces mobaraensis (Sm-ELA), which specifically catalyzes hydrolysis of the ε-amide bond in various Nε-acyl-l-lysines, was cloned and sequenced. The Sm-ELA gene consists of a 1617-bp open reading frame that encodes a 538-amino acid protein with a molecular mass of 55,816 Da. An NCBI protein-protein BLAST search revealed that the enzyme belongs to the YtcJ-like metal-dependent amidohydrolase family, which is further characterized as the metallo-dependent hydrolase superfamily. The Sm-ELA gene was ligated into a pUC702 vector for expression in Streptomyces lividans TK24. Expression of recombinant Sm-ELA in S. lividans was approximately 300-fold higher than that in ...
Bagramyan, K., et al. Mass Spectrometric Resurrection of SHA, an LRhamnose and Beta-D-Galactose Binding Lectin from the Lost Strain Streptomyces 27S5. ASMS. 0, ThP 307. 01/06/2015.. ...
Clavulanic acid. Its not an antibiotic in the classical sense of the word, but where would be without it? Probably one of the most important discoveries in the history of antibiotic development.. Clavulanic acid, or potassium clavulanate, was discovered in the early 1970s by scientists at Beecham pharmaceuticals. Produced naturally by the bacterium Streptomyces clavuligerus, it is a potent inhibitor of (Class A) beta-lactamases.. We know that Streptomyces are soil bacteria and there are hundreds of different Streptomyces species recorded in the taxonomy. A majority of Streptomyces species produce beta-lactam antibiotics and a lot of them also produce beta-lactamases, showing the lengths that these bacteria would go to to survive amongst other streptomyces and also other micro-organisms living in close proximity. It is interesting therefore that one of the Streptomyces species (Streptomyces clavuligerus) had the brains to produce a beta-lactamase inhibitor. Why? This would have given the ...
Mangrove Streptomyces represent a rich source of novel bioactive compounds in medicinal research. A novel alkaloid, named 1-N-methyl-3-methylamino-[N-butanoic acid-3¢-(9¢-methyl-8¢-propen-7¢-one)-amide]-benzo[f][1,7]naphthyridine-2-one (1) was isolated from Streptomyces albogriseolus originating from mangrove sediments. The structure of compound 1 was elucidated by extensive spectroscopic data analyses and verified by the 13C-NMR calculation at the B3LYP/6-311+G(2d,p) level of theory.
Genes encoding two ribonucleotide reductases (RNRs) were identified in members of the genus Streptomyces. One gene, nrdJ, encoded an oligomeric protein comprising four identical subunits each with a molecular mass of ∼108 kDa. The activity of this protein depended on the presence of 5′-deoxyadenosylcobalamine (coenzyme B12), establishing it as a class II RNR. The Streptomyces clavuligerus nrdJ gene was cloned, using internal peptide sequences from the purified protein, and was found to encode a polypeptide of 961 aa. Molecular phylogenetic analysis showed that the S. clavuligerus class II RNR shares significant similarity with most other bacterial and archaeal class II RNRs. Two other genes, nrdA and nrdB, were initially identified in the Streptomyces coelicolor genome database in unannotated ORFs as encoding a class Ia RNR. Southern analysis demonstrated that the nrdAB genes were present in different Streptomyces spp. The S. coelicolor nrdAB genes were cloned and expressed in Escherichia coli, and
ID H2K393_STRHJ Unreviewed; 512 AA. AC H2K393; DT 21-MAR-2012, integrated into UniProtKB/TrEMBL. DT 21-MAR-2012, sequence version 1. DT 22-NOV-2017, entry version 39. DE RecName: Full=Probable DNA ligase {ECO:0000256,HAMAP-Rule:MF_00407}; DE EC= {ECO:0000256,HAMAP-Rule:MF_00407}; DE AltName: Full=Polydeoxyribonucleotide synthase [ATP] {ECO:0000256,HAMAP-Rule:MF_00407}; GN Name=lig {ECO:0000256,HAMAP-Rule:MF_00407}; GN OrderedLocusNames=SHJG_2641 {ECO:0000313,EMBL:AEY87915.1}; OS Streptomyces hygroscopicus subsp. jinggangensis (strain 5008). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=1133850 {ECO:0000313,EMBL:AEY87915.1, ECO:0000313,Proteomes:UP000007170}; RN [1] {ECO:0000313,Proteomes:UP000007170} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=5008 {ECO:0000313,Proteomes:UP000007170}; RA Wu H., Bai L.; RT Genomic analysis of Streptomyces hygroscopicus subsp. jinggangensis RT 5008.; RL Submitted (JAN-2012) to the ...
The reactions of beta-lactamases of Actinomadura R39 and Streptomyces albus G with clavulanate proceed along branched pathways. Both enzymes perform the hydrolysis of this beta-lactam with rather high efficiencies (kcat. = 18s-1 and 52s-1 respectively). If large clavulanate/enzyme ratios are used, complete inactivation of the enzymes is observed. At lower ratios, inactivation is only partial. Irreversible inactivation occurs after 400 and 20000 turnovers for the A. R39 and S. albus G enzymes respectively. With the A. R39 beta-lactamase, a transiently inhibited complex is also formed that remains undetectable with the S. albus G beta-lactamase. Kinetic models are presented and studied for the interaction between clavulanate and both enzymes. A tentative general reaction scheme is also discussed.. ...
SUMMARY: Germinating spores of Streptomyces viridochromogenes excreted a substance into the surrounding medium which inhibited germination of another sample of the spores. The germination inhibitor (GI) was produced during submerged culture after exponential growth had ceased. The GI was purified 51-fold following extraction from growth liquor with chloroform. It was soluble in alcohol and water and had a molecular weight of less than 1000. The GI blocked growth and respiration of some Gram-positive bacteria and was an inhibitor of the membrane bound, but not solubilized, calcium-dependent ATPase of germinated spores and mycelia of the producing organism. Several sodium-potassium activated ATPases were also inhibited. All four activities (respiration, growth, germination inhibition. ATPase) co-purified during column and thin-layer chromatography. The GI activities released during germination and produced during growth were identical. A role for the GI antibiotic in regulation of dormancy of spores of
Streptomyces violaceoruber ATCC ® BAA-471™ Designation: John Innes Centre M145 TypeStrain=False Application: The parental strain, Streptomyces coelicolor A3(2), was used worldwide in genetic studies.
Coloured scanning electron micrograph (SEM) of Streptomyces hygroscopicus, Gram-positive, aerobic, filamentous, biofilm-forming, rod prokaryote (bacterium). Streptomyces sp. belong to the Actinomycetes group and are bacteria that share many characteristics with the fungi. They grow usually as filaments (chains of cells) and often branch to form a network of filaments (mycelium) in the soil. These soil bacteria are responsible for the musty odour of soil. This strain of Streptomyces hygroscopicus produces the antibiotic, milbemycin, which is used as an insecticide and also to control certain parasitic infections in animals. It is also a biofilm forming bacterium. Biofilms are primarily accumulations of bacteria in aqueous environments. They form when bacteria secrete slimy, mucilaginous materials that provide the microorganisms with a means of attachment to moist surfaces. Magnification: x1,600 when shortest axis printed at 25 - Stock Image C032/2295
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Streptomyces hygroscopicus 5008 has been used for the production of the antifungal validamycin/jinggangmycin for more than 40 years. A high yield of validamycin is achieved by culturing the strain at 37°C, rather than at 30°C for normal growth and sporulation. The mechanism(s) of its thermo-regulated biosynthesis was largely unknown. The 10,383,684-bp genome of strain 5008 was completely sequenced and composed of a linear chromosome, a 164.57-kb linear plasmid, and a 73.28-kb circular plasmid. Compared with other Streptomyces genomes, the chromosome of strain 5008 has a smaller core region and shorter terminal inverted repeats, encodes more α/β hydrolases, major facilitator superfamily transporters, and Mg2+/Mn2+-dependent regulatory phosphatases. Transcriptomic analysis revealed that the expression of 7.5% of coding sequences was increased at 37°C, including biosynthetic genes for validamycin and other three secondary metabolites. At 37°C, a glutamate dehydrogenase was transcriptionally up
Strains VIM M 10366(T), YIM M 10378(T) and YIM M 10400(T) were isolated from marine sediments collected from the Xisha Islands in the South China Sea. All three isolates were able to grow optimally at pH 7.0, 28-37 degrees C and 0-3% (w/v) NaCl. Comparison of 16S rRNA gene sequences showed that these strains are members of the genus Streptomyces, exhibiting moderately high 16S rRNA gene sequence similarities of 97.0-98.8% to members of the most closely related Streptomyces species. Morphological characteristics, physiological characteristics and compositions of whole-cell sugars and phospholipids are consistent with the diagnostic characteristics of the genus Streptomyces, but still allowed differentiation amongst the three strains and their neighbours. Based on 16S rRNA gene sequence analysis, DNA DNA relatedness, phenotypic characteristics and chemotaxonomic data, strains VIM M 10366(T), VIM M 10378(T) and VIM M 10400(T) were identified as members of three novel species of the genus ...
Read Properties of lanK-based regulatory circuit involved in landomycin biosynthesis in Streptomyces cyanogenus S136, Russian Journal of Genetics on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Taxonomy of the species Streptomyces bambergiensis Wallhäusser et al. 1966 (Approved Lists 1980) pro synon. Streptomyces prasinus Ettlinger et al. 1958 (Approved Lists 1980) emend. Labeda et al. 2016
The cancer-cell-cytotoxicity-guided fractionation of the acetone extracts of two cultured marine-derived Streptomyces strains belonging to the MAR4 group yielded six new napyradiomycins, compounds A-F (1-6), together with three known compounds, napyradiomycins B2-B4 (7-9). Napyradiomycins 1-4 are new members of the napyradiomycin C-type meroterpenoids, which possess a linear monoterpene bridge between C-7 and C-10a. Compound 4 has an additional tetrahydropyran ring fused to the phenol moiety. Compounds 5-9 are related to the napyradiomycin B-type meroterpenoids. The structures of all new compounds were assigned by interpretation of 1D and 2D NMR, MS, and other spectroscopic data. The relative configurations were assigned based upon interpretation of ROESY 2D NMR experiments. The cytotoxicity of 1-9 against the human colon carcinoma cell line HCT-116 and their antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA) are presented.. ...
Streptomyces violaceoruber ATCC ® BAA-471D-5™ Designation: Genomic DNA from M145 (ATCC BAA-471) TypeStrain=False Application:
Spectrophotometric determination of the ionophore compounds and mass spectrometry of the biosynthesis products revealed that the cells of Streptomyces chrysomallus subsp. macrotetrolidi during the lag phase permanently contained practically constant though insignificant (about 20 to 40 nmol/mg of protein) amounts of biosynthetic precursors of macrotetrolides, oligomers of nactinic acids. The oligomers of nactinic acids had antibiotic activity and in an amount of 2.5 micrograms/ml inhibited the growth of Bacillus mycoides. The oligomers of nactinic acids had ionophore properties and were highly labile with respect to inorganic cations. The presence of sodium in the extragent stabilized the calcium monomer, a trimer of nonactinic acid, and promoted washing off the substances of the nactinic nature from the cells. The cations of ammonia and possibly potassium stabilized the dimer and tetramer of nonactinic acid forming a more hydrophobic complex by comparison with the initial compounds.
Streptomyces peucetius DnrS protein: involved in daunorubicin biosynthesis in Streptomyces peucetius; encodes a glycosyltransferase; amino acid sequence given in first source; GenBank L47164
BLEOCIN™ Streptomyces verticillus, Sterile-Filtered, Aqueous Solution - CAS 55658-47-4 - Calbiochem CAS 55658-47-4 A unique antibiotic of the bleomycin family that is toxic to eukaryotic and prokaryotic cells. - Find MSDS or SDS, a COA, data sheets and more information.
Original publication: Krainsky A. Die Aktinomyceten und ihre Bedeutung in der Natur. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abteilung II 1914; 41:649-688. Nomenclatural status: not validly published Correct name: Streptomyces diastatochromogenes (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980) ...
FR-900520 and FR-900523, novel neutral macrolide immunosuppressants, were isolated from the cultured broth of Streptomyces hygroscopicus subsp. yakushimaensis No. 7238. Their molecular formulae were determined as C43H69NO12 and C42H67NO12, respectively. The compounds suppressed immune response in vitro. IC50 values of FR-900520 and FR-900523 for mouse mixed lymphocyte reaction were 0.55 nM and 1.6 nM, respectively. FR-900520, the major component, clearly prolonged skin allograft survival in rats.
TY - JOUR. T1 - 12T061A and 12T061C, two new julichrome family compounds, as radical scavengers from Streptomyces sp. AU - Komoda, Toshikazu. AU - Saeki, Naoko. AU - Koseki, Yoshitaka. AU - Kiyota, Hiromasa. PY - 2016/2/29. Y1 - 2016/2/29. N2 - We identified two new radical scavengers, 12T061A (1, C19H20O7) and 12T061C (2, C20H22O7), from a culture of the Streptomyces sp. Spectroscopic analysis indicated that these compounds are new julichrome family compounds. Compounds 1 and 2 showed radical-scavenging activity with an ED50 of 370 μM and 18 μM, respectively. Moreover, 1 showed tumor cell growth suppressive activity in HepG2 cells, (IC50: 3.6 μM); however, no suppressive activity was shown in 2 (IC50: , 100 μM).. AB - We identified two new radical scavengers, 12T061A (1, C19H20O7) and 12T061C (2, C20H22O7), from a culture of the Streptomyces sp. Spectroscopic analysis indicated that these compounds are new julichrome family compounds. Compounds 1 and 2 showed radical-scavenging activity ...
als Synonym von Streptomyces setonii identifiziert,[7] so dass es sich nun nach aktueller Systematik um Streptomyces setonii (Millard & Burr 1926) Waksman 1953 emend. Antibiotikum, Die Arten dieser Gattung sind grampositiv, aerob, Myzel-bildend, mehrzellig, bilden Sporen und haben einen hohen GC-Gehalt. Short characterization of Streptomyces chromofuscus 34-1. Es beinhaltet die meisten Gene aller bisher untersuchten Bakterien. The spores were elongated with smooth surface (Fig. X‐119‐6, which catalyzes the oxidative deamination of l ‐glutamate, has attracted increasing attention as a component of amperometric l ‐glutamate sensors used in the food industry and clinical biochemistry. Sie benötigen für ihr… Michael T. Madigan, John M. Martinko, Jack Parker: E. A. Bayer, T. Kulik, R. Adar, M. Wilchek: H. Hager, F. v. Bruchhausen, R. Batty, G. Wurm: H. Drautz, W. Keller-Schierlein, H. Zähner: internationalen Code der Nomenklatur von Bakterien, Leibniz Institut DSMZ - Deutsche Sammlung ...
11181344] An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57. (Antimicrob Agents Chemother. , 2001 ...
Name: Streptomyces halstedii (Waksman and Curtis 1916) Waksman and Henrici 1948 (Approved Lists 1980). Category: Species. Proposed as: comb. nov.. Basonym: Actinomyces halstedii Waksman and Curtis 1916 Etymology: hal.stedi.i. N.L. gen. masc. n. halstedii, of Halsted, named for Professor Halsted of Rutgers University Gender: masculine Type strain: ATCC 10897; ATCC 19770; BCRC 13680; CBS 508.68; CCRC 13680; CECT 3328; DSM 40068; HAMBI 993; IFO 12783; IMET 40322; JCM 4584; NBRC 12783; NCIB 9839; NCIMB 9839; NRRL B-1238; NRRL ISP-5068; RIA 1050; UNIQEM 156; VKM Ac-1768 See detailed strain information at ...
Macro-photograph of a colony of the soil bacterium Streptomyces lividans. Streptomyces species are natural producers of a number of antibiotics, including streptomycin. A member of the Actinomycetes group, they share many characteristics with the fungi. The bacteria grow in the soil as a branching network of filaments, known as a mycelium. They also produce aerial mycelium, from the tips of which develop chains of spores. When the spores are mature, they rupture and are dispersed on the wind. - Stock Image B240/0019
TY - JOUR. T1 - Roles of the signal peptide and mature domains in the secretion and maturation of the neutral metalloprotease from Streptomyces cacaoi. AU - Chang, Su Chih. AU - Su, Mei Han. AU - Wu Lee, Yan-Hwa. PY - 1997/1/1. Y1 - 1997/1/1. N2 - The neutral metalloprotease (Npr) of Streptomyces cacaoi is synthesized as a prepro-Npr precursor form consisting of a secretory signal peptide, a propeptide and the mature metalloprotease. The maturation of Npr occurs extracellularly via an autoproteolytic processing of the secreted pro-Npr. The integrity of the propeptide is essential for the formation of mature active Npr but not for its secretion. In this study we investigated whether the secretion and maturation of Npr require the integrity of its signal peptide region and mature protease domain. Five signal peptide mutants were generated, including the substitution mutations at the positively charged region (mutant 1R6LE), the central hydrophobic region (mutants GI19EL and G19N), the boundary of ...
High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherechia coli. Isolation and antifungal and antioomycete activities of aerugine produced by Pseudomonas fluorescens strain MM-B16
Sequence analysis of a 3.4-kb region Streptomyces peucetius daunorubicin (DNR) gene cluster established the presence of the dnrH and dnmT genes. In dnrH mutants, DNR production increased 8.5-fold, compared with that in the wild-type strain, while dnmT mutants accumulated epsilon-rhodomycinone (RHO), which normally becomes glycosylated in daunorubicin biosynthesis. Hence, dnmT may be involved in the biosynthesis or attachment of daunosamine to RHO or in the regulation of this process. Since the DnrH protein is similar to known glycosyl transferases, this protein may catalyze the conversion of DNR to its polyglycosylated forms, known as baumycins. Overexpression of dnmT in the wild-type and dnrH mutant strains resulted in a major decrease in RHO accumulation and increase in DNR production. ...
The genome of Streptomyces encodes a high number of natural product (NP) biosynthetic gene clusters (BGCs). Most of these BGCs are not expressed or are poorly expressed (commonly called silent BGCs) under traditional laboratory experimental conditions. These NP BGCs represent an unexplored rich reservoir of natural compounds, which can be used to discover novel chemical compounds. To activate silent BGCs for NP discovery, two main strategies, including the induction of BGCs expression in native hosts and heterologous expression of BGCs in surrogate Streptomyces hosts, have been adopted, which normally requires genetic manipulation. So far, various genome editing technologies have been developed, which has markedly facilitated the activation of BGCs and NP overproduction in their native hosts, as well as in heterologousStreptomyces hosts. In this review, we summarize the challenges and recent advances in genome editing tools for Streptomyces genetic manipulation with a focus on editing tools ...
TO THE EDITOR: Streptomyces spp. are aerobic, gram-positive bacteria of the order Actinomycetales, known for their ability to produce antimicrobial molecules such as streptomycin. Streptomyces spp., usually saprophytic to humans, can cause local cutaneous fistulized nodules known as actinomycetoma or mycetoma. Severe invasive infections have seldom been reported, but most cases reported have occurred in immunocompromised patients (1-5). We report a case of invasive pulmonary infection caused by a Streptomyces sp. in a splenectomized patient with sarcoidosis ...
Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics.(Chem. Biol.) [2001] ...
The enzyme, characterized from the bacterium Streptomyces hygroscopicus subsp. limoneus, is involved in the biosynthesis of the antifungal agent validamycin A.
Bio - Nata is produced from Streptomyces lydicus, a naturally occurring bacterium that is commonly found in soil environment. Bio - Nata is produced f...
Fourteen Streptomyces strains were isolated from soil samples of five different areas of Cukurova University. Four strains (S35, S47, S48, S50) had antibacterial activity against test organisms which were Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa and this antibacterial activity was evaluated by three different media. The strains S47 and S48 showed the biggest zone diameter against test organisms at glycerol-yeast agar medium, on the other hand, the strains S35 and S50 indicated the biggest zone diameter at glucose-asparagine agar medium. As a result, the group and activity of bioactive compounds produced by Streptomyces can be influenced by their nutritional conditions and environmental factors. ...
1) Streptomyces coelicolor A3(2). NCBI Taxonomy Browser. 29 April 2007. NCBI. (2) Conn, Jean E. The Pigment Production of Actinomyces coelicolor and A. violaceus-ruber. Journal of Bacteriology. 1943. Volume 46. p. 133-149. Link to Article (3) From Mapping to Mining the Streptomyces Genome. John Innes Centre Website. 2001. Link to Article (4) Thompson, Charles J., Dorris Fink, and Liem D. Nguyen. Principles of Microbial Alchemy: Insights from the Streptomyces coelicolor Genome Sequence. Genome Biology 3.7. (2002) Link to Article on PubMed (5) Kutzner, Hans J and Selman A. Waksman. Streptomyces coelicolor Muller and Streptomyces violaceoruber Waksman and Curtis, Two Distinctly Different Organisms. Journal of Bacteriology 78.4 (1959) p. 528-538. Link to Article (6) Bentley, S.D., K. F. Chater, A.-M. Cerdeño-Tárraga, G. L. Challis , N. R. Thomson, K. D. James, D. E. Harris, M. A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C. W. Chen, M. Collins, A. Cronin, A. Fraser, ...
Streptomyces scabies or Streptomyces scabiei is a streptomycete bacterium species found in soils around the world. Unlike most of the 500 or so Streptomyces species it is a plant pathogen causing corky lesions to form on tuber and root crops as well as decreasing the growth of seedlings. Along with other closely related species it causes the potato disease common scab, which is an economically important disease in many potato growing areas. It was first described in 1892, being classified as a fungus, before being renamed in 1914 and again in 1948. Several other species of Streptomyces cause similar diseases to S. scabies but other, more closely related species, do not. The genome of S. scabies has been sequenced and is the largest Streptomyces genome known so far. The genome contains a pathogenicity island containing the genes required for S. scabies to infect plants, and which can be transferred between different species. S. scabies can produce several related toxins which are the most ...
en] The simplest model for the interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics involves the three following steps: (a) the formation of a reversible equimolar enzyme - antibiotic complex; (b) the irreversible transformation of this complex into a modified enzyme - antibiotic complex; and (c) the breakdown of this latter complex and the concomitant release of a regenerated enzyme and a modified antibiotic molecule. The dissociation constant for step 1 and the rate constants for steps 2 and 3 were measured with various beta-lactam antibiotics. With antibiotic such as benzylpenicillin, which behaves as a good substrate, steps 1 and 2 occur at enzymic velocities, whereas step 3 occurs at a very low velocity and hence is responsible for the low efficiency of the overall process ...
Bleomycin is a broad-spectrum glycopeptide antitumor antibiotic produced by Streptomyces verticillus. Clinically, the mixture of bleomycin A2 and bleomycin B2 is widely used in combination with other drugs for the treatment of various cancers. As a secondary metabolite, the biosynthesis of bleomycin is precisely controlled by the complex extra-/intracellular regulation mechanisms, it is imperative to investigate the global metabolic and regulatory system involved in bleomycin biosynthesis for increasing bleomycin production. N-acetylglucosamine (GlcNAc), the vital signaling molecule controlling the onset of development and antibiotic synthesis in Streptomyces, was found to increase the yields of bleomycins significantly in chemically defined medium. To mine the gene information relevant to GlcNAc metabolism, the DNA sequences of dasR-dasA-dasBCD-nagB and nagKA in S. verticillus were determined by chromosome walking. From the results of Real time fluorescence quantitative PCR (RT-qPCR) and
Streptomyces species are considered exceptionally well endowed for chemical warfare, presumably allowing them to eliminate bacterial and fungal competitors in soil ecosystems. This was first realized over fifty years ago, and since then the majority of known antibiotics have been isolated from Streptomyces. Although thousands of antibiotics have been described, these are thought to represent only a small fraction of the repertoire of bioactive compounds produced by Streptomyces [4,5]. In addition, empirical screening using various assays has revealed that Streptomyces culture supernatants contain other pharmaceutically active compounds, such as anti-viral and anti-cancer compounds, modulators of immune responses, and various enzyme inhibitors, as well as herbicides, insecticides, and anti-parasitic compounds [4,6,7]. Furthermore, chemical screening methods have uncovered the remarkable structural diversity of these compounds. Genome sequences of Streptomyces species are now providing an ...
Streptomyces globisporus 1912 lnd-cluster region which flanks structural lndZ5-lndZ6 genes contains four open reading frames lndW, lndW2, lndYR and lndY. The latter one encodes putative proteinase which can regulate landomycin production and morphogenesis like LndYR. However, results of lndY overexpression and gene knockout showed that lndY did not participate in regulation of landomycin production and morphogenesis of Streptomyces globisporus 1912. Using transcriptional fusion of promoter lndWp to catechol dioxygenase reporter gene xylE the temporal character of interaction of promoter lndWp and repressor LndYR was studied.. ...
Detail záznamu - tmRNA of Streptomyces collinus and Streptomyces griseus during the growth and in the presence of antibiotics - Detail záznamu - Knihovna Akademie věd České republiky
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology
To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in the food industry, the atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mobaraensis mutants with increased TGase production. After eight rounds of iterative ARTP mutagenesis, four genetically stable mutants, Sm5-V1, Sm6-V13, Sm2-V10, and Sm7-V12, were identified, which showed increased TGase production by 27, 24, 24, and 19%, respectively. The best mutant Sm5-V1 exhibited a maximum TGase activity of 5.85 U/mL during flask fermentation. Compared to the wild-type strain, the transcription levels of the zymogen TGase genes in the mutants increased significantly as indicated by quantitative real-time PCR, while the gene nucleotide sequences of the mutants did not change at all. It was shown that the overexpression of TGase zymogen gene in the mutants contributes to the increase in TGase production. ARTP is a potentially efficient tool for microbial mutation
TY - JOUR. T1 - TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2). AU - Puglia, Anna Maria. AU - Botta, Luigi. AU - Giardina, Anna. AU - Gallo, Giuseppe. AU - Sutera, Alberto. AU - Palazzotto, Emilia. AU - Scaloni, Andrea. AU - Renzone, Giovanni. AU - Palazzotto, Emilia. PY - 2016. Y1 - 2016. N2 - In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain. The capability of this mutant to grow on minimal medium was ...
Self-resistance of the nourseothricin-producing strain Streptomyces noursei.: The nourseothricin producer Streptomyces noursei is resistant to its own antibioti
Malate synthases (MS) from Streptomyces coelicolor A3(2) and S. clavuligerus NRRL3585 were cloned by polymerase chain reaction into a glutathione S-transferase (GST) fusion expression vector and heterologously expressed in Escherichia coli. The fusion GST-MS construct improved the soluble expression of MS by approximately 10-fold compared to the soluble expression of nonfusion MS. With the significant improvement in levels of soluble MS, purification and subsequent cleavage of recombinant MS from GST were facilitated in this study. Using purified enzymes, optimized parameters, which achieved maximal specific activity, were established in the enzymatic assay for streptomycete MS. The average purified specific activities of S. coelicolorand S. clavuligerus MS were 26199 and 11821 nmol/mg min, respectively. Furthermore, enzymatic analysis revealed that the two streptomycete MS displayed a similar Km value for acetyl-CoA, but S. coelicolor MS had a Km value for glyoxylate that is approximately ...
Amicetin, an antibacterial and antiviral agent, belongs to a group of disaccharide nucleoside antibiotics featuring an alpha-(1 -> 4)-glycoside bond in the disaccharide moiety. In this study, the amicetin biosynthesis gene cluster was cloned from Streptomyces vinaceusdrappus NRRL 2363 and localized on a 37-kb contiguous DNA region. Heterologous expression of the amicetin biosynthesis gene cluster in Streptomyces lividans TK64 resulted in the production of amicetin and its analogues, thereby confirming the identity of the ami gene cluster. In silico sequence analysis revealed that 21 genes were putatively involved in amicetin biosynthesis, including 3 for regulation and transportation, 10 for disaccharide biosynthesis, and 8 for the formation of the amicetin skeleton by the linkage of cytosine, p-aminobenzoic acid (PABA), and the terminal (+)-alpha-methylserine moieties. The inactivation of the benzoate coenzyme A (benzoate-CoA) ligase gene amiL and the N-acetyltransferase gene amiF led to two ...
Organofluorine compounds are widely prepared throughout the chemicals industry, but their prepararion generally requires harsh fluorinating reagents and non-aqueous solvents. On the other hand, biology has hardly exploited organofluorine compounds. A very few organisms synthesize organofluorine metabolites, suggesting they have evolved a mechanism to overcome the kinetic desolvation barrier to utilizing F(-)(aq). Here, the purification and crystallization of an enzyme from Streptomyces cattleya which is responsible for the synthesis of the C-F bond during fluoroacetate and 4-fluorothreonine biosynthesis is reported. The protein crystallizes in space group C222(1), with unit-cell parameters a = 75.9, b = 130.3, c = 183.4 A, alpha = beta = gamma = 90 degrees. Data were recorded to 1.9 A at the ESRF. The structure of the protein should provide important insights into the biochemical process of C-F bond formation.. ...
The impact of different carbon sources on the antibiotic production by Streptomyces hygroscopicus CH-7 was studied with the main goal to increase the yield of antibiotics hexaene H-85 and elaiophylin. Glucose, as a basic carbon source in the nutrition medium, was replaced with glycerol, xylose, sorbose, melibiose, inulin, and mannitol (15 g/dm(3)). Insuring the maximum yields of hexaene and elaiophylin of 192 and 88 mu g/cm(3), respectively, glycerol was shown to be the best carbon source among the investigated ones ...
TY - JOUR. T1 - New non-quinone geldanamycin analogs from genetically engineered Streptomyces hygroscopicus. AU - Wu, Cheng Zhu. AU - Moon, An Na. AU - Jang, Jae Hyuk. AU - Lee, Dongho. AU - Kang, Sun Young. AU - Park, Joon Tae. AU - Ahn, Jong Seog. AU - Hwang, Bang Yeon. AU - Kim, Young Ho. AU - Lee, Hong Sub. AU - Hong, Young Soo. N1 - Funding Information: This work was supported in part by the 21C Frontier Microbial Genomics and Application Center and Global R&D Center program, the Ministry of Science and Technology, Republic of Korea and by a grant from KRIBB Research Initiative Program. We thank the Korea Basic Science Institute for the NMR measurements. PY - 2011/6. Y1 - 2011/6. KW - Hsp90 inhibitor. KW - biosynthetic engineering. KW - geldanamycin. KW - non-quinone geldanamycin. UR - http://www.scopus.com/inward/record.url?scp=79959736928&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=79959736928&partnerID=8YFLogxK. U2 - 10.1038/ja.2011.24. DO - ...
g- 1, 6 NAKAO ISHIDA ETAL 3,334,022 NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES CARZINOSTATICUS VAR NEOCARZINOSTATICUS Filed April 26, 1965 2 Sheets-Sheet 1 NAKAO ISHIDA KEIZO MIYAZAKI KATSUO KUMAGAI MITSUO RIKIMARU MA SAl-HKO KUROYA INVENTORS. B. SIMONTON & HERBERT W TAYLOR,JR. ATTORNEYS. NAKAO-ISHIDA ETAL NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES Aug. l 1967 3,334,022 NOSTATICUS VAR. NEOCARZINOSIATICUS v CARZI Filed April 26, 1965 2 Sheets-Sheet O O O O O O O om D P a f P Om \r fl cm 09 PERCENT TRANSMISSION IUA IAR KGAW AA M WK W Y IKRO OO O UUH AO A QTT AEA NKKMM N w w 2% K ON N R IOYO M T ABIT H WA W RWR TMEK R BR CRRH Y B United States Patent 3,334,022 NEOCARZINOSTATIN PRODUCED BY STREPTOMYCES CARZIZVOSTATIC US VAR. NEOCARZINOSTATICUS Nakao Ishida, 90 Kakogoro-cho; Keizo Miyazaki, 48 Kakyoin-dori; Katsuo Kumagai, 129 Tori-cho; and Mitsuo Rikimaru, 61 Kita-shichiban-cho, all of Sendai, Japan; and Masahiko Kuroya, Room 605, Empire Corp., 28 Daikyo-machi, Shinjuku-ku, Tokyo, Japan Filed Apr. ...
A homologue of the bacterial cell division gene ftsZ was cloned from the filamentous bacterium Streptomyces coelicolor. The gene was located on the physical map of the chromosome at about 11 oclock (in the vicinity of glkA, hisA and trpB). Surprisingly, a null mutant in which the 399-codon ftsZ open reading frame was largely deleted was viable, even though the mutant was blocked in septum formation. This indicates that cell division may not be essential for the growth and viability of S. coelicolor. The ftsZ mutant was able to produce aerial hyphae but was unable to produce spores, a finding consistent with the idea that ftsZ is required in order for aerial hyphae to undergo septation into the uninucleoid cells that differentiate into spores. ...
Cell division in the Gram-positive bacterium Streptomyces coelicolor starts with the assembly of the tubulin homologue FtsZ into a cytokinetic ring (the Z ring) at the site of septation. In stark contrast to the binary fission of most bacteria, the syncytial hyphal cells of S. coelicolor exploit two types of cell division with strikingly different outcomes depending on the developmental stage. The main goal of this study has been to identify developmental mechanisms that modulate this differential performance of the basic cell division machinery.. By isolation and characterization of a non-sporulating ftsZ mutant, we demonstrated that the requirements for Z-ring formation differ between the two types of septation. The ftsZ17(Spo) mutation abolished septation without overtly affecting vegetative growth. This mutant was defective in the assembly of FtsZ into regularly spaced Z rings in sporogenic hyphae, suggesting that the assembly of Z rings is developmentally controlled during ...
TY - JOUR. T1 - The ADEP biosynthetic gene cluster in Streptomyces hawaiiensis NRRL 15010 reveals an accessory clpP gene as a novel antibiotic resistance factor. AU - Thomy, Dhana. AU - Culp, Elizabeth. AU - Adamek, Martina. AU - Cheng, Eric Y.. AU - Ziemert, Nadine. AU - Wright, Gerard D.. AU - Sass, Peter. AU - Brötz-Oesterhelt, Heike. PY - 2019. Y1 - 2019. N2 - The increasing threat posed by multiresistant bacterial pathogens necessitates the discovery of novel antibacterials with unprecedented modes of action. ADEP1, a natural compound produced by Streptomyces hawaiiensis NRRL 15010, is the prototype for a new class of acyldepsipeptide (ADEP) antibiotics. ADEP antibiotics deregulate the proteolytic core ClpP of the bacterial caseinolytic protease, thereby exhibiting potent antibacterial activity against Gram-positive bacteria, including multiresistant pathogens. ADEP1 and derivatives, here collectively called ADEP, have been previously investigated for their antibiotic potency against ...
The antifungal polyene macrolide nystatin is produced by Streptomyces noursei ATCC 11455. The nystatin biosynthesis gene cluster of Streptomyces noursei has been cloned and sequenced, and a biosynthesis route has been predicted. In the present work, investigation of genes presumably involved in post-PKS modifications of nystatin is described. The aim of this work was to better understand the nystatin biosynthesis and to further use this information for generation of novel nystatin analogues. Two PKS-modifications of the nystatin molecule were targeted in this study: glycosylation with mycosamine at C-19 and oxidation of the exocyclic methyl group at C-16.. Two genes putatively involved in mycosamine biosynthesis (NysDIII and NysDII) and one in attachment of mycosamine to the nystatin aglycone (nysDI) have been identified in the nystatin gene cluster. Their functions have been suggested, respectively, as a putative mannose dehydratase, aminotransferase and a glycosyltransferase. The deoxysugar ...
Streptomyces sp. belongs to Streptomyces, the largest genus of Actinobacteria and the type genus of the family Streptomycetaceae.
In Streptomyces coelicolor, bldA encodes the only tRNA for a rare leucine codon, UUA. This tRNA is unnecessary for growth, but is required for some aspects of secondary metabolism and morphological development. We describe a transcriptomic and proteomic analysis of the effects of deleting bldA on cellular processes during submerged culture: conditions relevant to the industrial production of antibiotics. At the end of rapid growth, a co-ordinated transient up-regulation of about 100 genes, including many for ribosomal proteins, was seen in the parent strain but not the ΔbldA mutant. Increased basal levels of the signal molecule ppGpp in the mutant strain may be responsible for this difference. Transcripts or proteins from a further 147 genes classified as bldA-influenced were mostly expressed late in culture in the wild-type, though others were significantly transcribed during exponential growth. Some were involved in the biosynthesis of seven secondary metabolites; and some have probable roles in
Streptomyces are a genus of filamentous bacteria that includes over 500 species characterized by a fungal like mycelium body and the production of spores for reproduction. Found predominantly in soil and decaying vegetation, they are a major source of antibiotics (which is why Ginkgo studies them) and produce geosmin, a metabolite that gives soil its characteristic earthy rain smell; many species in the genus also produce vivid pigments as metabolites. Natsai Audrey Chieza, Ginkgos first creative resident used Streptomyces coelicolor to naturally dye silk, by culturing the bacteria directly onto the textile in different ways to produce different patterns. Ginkgo provided me with different Streptomyces cultures to test on my bioplastics and see if they could be cultured to dye the materials, transforming them as they also decomposed them. Kyle taught me how to successfully culture S. coelicolor in sterile conditions onto plain cellulose (rice paper) and thin wood substrates however it was ...
Streptomyces lividans CelB is a family-12 endoglucanase that hydrolyses cellulose with retention of anomeric configuration. A recent X-ray structure of the catalytic domain at 1.75 Å resolution has led to the preliminary assignment of Glu-120 and Glu-203 as the catalytic nucleophile and general acid-base respectively [Sulzenbacher, Shareck, Morosoli, Dupont and Davies (1997) Biochemistry 36, 16032-16039]. The present study confirms the identity of the nucleophile by trapping the glycosyl-enzyme intermediate with the mechanism-based inactivator 2´,4´-dinitrophenyl 2-deoxy-2-fluoro-β-d-cellobioside (2FDNPC). The kinetics of inactivation proceeded in a saturable fashion, yielding the parameters kinact = 0.29±0.02 min-1 and Kinact = 0.72±0.08 mM. Uncompetitive inhibition was observed at high concentrations of 2FDNPC (Ki = 9±1 mM), a behaviour that was also observed with the substrate 2´,4´-dinitrophenyl β-d-cellobioside (kcat = 40±1 s-1, Km = 0.35±0.03 mM, Ki = 24±4 mM). Protection ...
Classification of Raman spectra recorded from single cells is commonly applied to bacteria that exhibit small sizes of approximately 1 to 2 μm. Here, we study the possibility to adopt this classification approach to filamentous bacteria of the genus ,i,Streptomyces,/i,. The hyphae can reach extensive lengths of up to 35 μm, which can correspond to a single cell identified in light microscopy. The classification of Raman bulk spectra will be demonstrated. Here, ultraviolet resonance Raman (UV RR) spectroscopy is chosen to classify six ,i,Streptomyces,/i, species by the application of a tree-like classifier. For each knot of the hierarchical classifier, estimated classification accuracies of over 94% are accomplished. In contrast to the classification of bulk spectra, the classification of single-cell spectra requires a homogenous substance distribution within the cell. Consequently, the bacterial cell chemistry can be represented by one individual spectrum. This requirement is not fulfilled ...
Bringmann, Gerhard and Lang, Gerhard and Maksimenka, Katja and Hamm, Andreas and Gulder, Tobias A. M. and Dieter, Anke and Bull, Alan T. and Stach, James E.M. and Kocher, Niko and Muller, Werner E. G. and Fiedler, Hans-Peter (2005) Gephyromycin, the first bridged angucyclinone, from Streptomyces griseus strain NTK 14. Phytochemistry, 66 (11). pp. 1366-1373. ISSN 0031-9422 . (doi:https://doi.org/10.1016/j.phytochem.2005.04.010 ) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) ...
Streptomyces coelicolor colonies from higher education and researchin the Microbes section. Germs and bacteria a catalog of all known germs
Streptomyces spp. have developed complicated mechanisms to adapt their metabolism to the change of the extracellular environments (16). Among these mechanisms, cross talk between different regulators may integrate different signal inputs through fine-tuning the expression of key target genes (10, 15). A regulatory link between nitrogen metabolism and phosphate metabolism was proposed in S. coelicolor, which was coordinated by PhoP through directly binding to the promoters of the nitrogen metabolism-associated genes, including glnA, glnII, and amtB (14). However, the roadblock mechanism suggested for amtB was different from that of the proposed competitive binding for glnA and glnII.. Based on the DNase I footprinting data, we defined, in the present study, a new GlnR binding box comprising of an a3-b3 site in addition to the previously well characterized a1-b1 and a2-b2 sites in the promoter region of amtB (22). All of the three GlnR binding boxes were proven essential for GlnR-mediated ...
1JFR: Structure of a microbial homologue of mammalian platelet-activating factor acetylhydrolases: Streptomyces exfoliatus lipase at 1.9 A resolution.
Streptomyces sp. ZZ035 isolated from a folk medicinal soil sample in China showed remarkable antimicrobial activities. During the isolation of secondary metabolites, a white crystal powder (1) was isolated from the broth of this strain. Its nuclear magnetic resonance (NMR) and infrared (IR) spectra indicated that it was a complex composed of triglycerides. Next, six C15-17 long- chain fatty acids derived from these triglycerides were respectively identified as n-pentadecanoyl, 12-methyltetradecanoyl, 14-methyl pentadecanoyl, palmitoyl, 15-methyl hexadecanoyl and 14-methyl hexadecanoyl using the gas chromatography-mass spectroscopy (GC-MS) technology. Finally, the 13C and 1H assignments of 1 were achieved through the analyses of NMR data. Based on above, their detailed NMR spectroscopic elucidation and meticulous 13C, 1H assignments, especially the split peaks and coupling correlation of protons attached on the glycerol carbons, were performed for distinguishing triglycerides from other glycerides and
Seven new azalomycin F analogs (1-7) were isolated from the broth of mangrove Streptomyces sp. 211726, and respectively identified as 25-malonyl demalonylazalomycin F5a monoester (1), 23-valine demalonylazalomycin F5a ester (2), 23-(6-methyl)heptanoic acid demalonylazalomycins F3a ester (3), F4a ester (4) and F5a ester (5), 23-(9-methyl)decanoic acid demalonylazalomycin F4a ester (6) and 23-(10-methyl)undecanoic acid demalony lazalomycin F4a ester (7). Their structures were established by their spectroscopic data and by comparing with those of azalomycins F3a, F4a and F5a. Biological assays exhibited that 1-7 showed broad-spectrum antimicrobial and anti HCT-116 activities.
Sigma-Aldrich offers Sigma-A2411, Amphotericin B from Streptomyces sp. for your research needs. Find product specific information including CAS, MSDS, protocols and references.
ID S5VSK1_STRC3 Unreviewed; 468 AA. AC S5VSK1; DT 16-OCT-2013, integrated into UniProtKB/TrEMBL. DT 16-OCT-2013, sequence version 1. DT 25-OCT-2017, entry version 33. DE RecName: Full=Glutamate decarboxylase {ECO:0000256,RuleBase:RU361171}; DE EC= {ECO:0000256,RuleBase:RU361171}; GN ORFNames=B446_20640 {ECO:0000313,EMBL:AGS70940.1}; OS Streptomyces collinus (strain DSM 40733 / Tu 365). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=1214242 {ECO:0000313,EMBL:AGS70940.1, ECO:0000313,Proteomes:UP000015423}; RN [1] {ECO:0000313,Proteomes:UP000015423} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 40733 / Tu 365 {ECO:0000313,Proteomes:UP000015423}; RA Ruckert C., Szczepanowski R., Goesmann A., Pross E.K., Musiol E.M., RA Blin K., Wohlleben W., Puhler A., Weber T., Kalinowski J.; RT The complete genome sequence of Streptomyces collinus Tu 365.; RL Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ...
Breast cancer is the most common form of cancer in Canadian women. Despite numerous therapeutic options, multidrug resistance (MDR) remains an obstacle to successful therapy.. Jadomycins are natural products derived from the soil bacteria Streptomyces venezuelae ISP5230 and maintain cytotoxicity in multidrug resistant human breast cancer cell lines. The objectives were to determine the single dose pharmacokinetics and safety of jadomycins and their effects on 4T1 breast primary tumors and lung metastasis in mice.. In the pharmacokinetic studies, intraperitoneal-administered jadomycins B, S, and F were rapidly absorbed, achieved serum concentration in the predicted therapeutic range and had a biphasic distribution and elimination profile. In tumor studies jadomycin B partially reduced tumor volumes and lung metastasis. In conclusion, jadomycins are safe to administer chronically to mice at doses up to 13.8 mg/kg twice daily. The partial anti-tumor and anti-metastatic effects of jadomycin B were ...
Novel compounds are produced by the fermentation of a nutrient medium with the previously undescribed microorganism Streptomyces avermitilis. They may be isolated by solvent extraction and chromatographic fractionation techniques. The compounds, which are described generically as C-076 have significant parasiticidal activity. The compounds may be included in compositions for the oral or parenteral administration to animals for the prevention and cure of parasitic infections.
The publication of the S. coelicolor genome sequence has stimulated interest in the possibility of rationally engineering strains by predicting the effect(s) of altering specific genes on secondary metabolite production. The work described here aims to design a general approach that could be applied to enhance the production of commercially important antibiotics that have similar biosynthetic routes as actinorhodin (ACT). It has been observed that a negative correlation between ACT production and the carbon flux through the pentose phosphate pathway (PPP) exists. As a result, strains with deletions for either one of the two zwf isogenes (encoding the enzyme that catalyses the initial PPP reaction) and for the devB gene (whose product catalyses the subsequent reaction in the pathway) were constructed and assessed. All strains, with the exception of the devB mutant, were found to generate increased levels of ACT compared to the parental strain. In addition to these strains, the above genotypes ...
Matt Hutchings Key Research Interests We are interested in how bacteria interact with the environment and how they sense and respond to environmental signals. All bacteria contain a cell envelope which consists of at least one cell membrane and (in most cases) a cell wall. Bacteria interact with their environment using cell surface proteins which are anchored either in the cell wall or the cell membrane. We are characterising two classes of these proteins in the filamentous bacteria Streptomyces. The first class are called sensor kinases and they span the membrane and transmit signals from the outside to the inside of the cell. They pass these signals to cognate response regulator proteins inside the cell and these bring about a response to the original signal, usually by altering gene expression. The second class are called lipoproteins and are attached by a lipid modification to the outside of the cell membrane. They have diverse roles including the scavenging of nutrients, surface attachment, ...
Streptomyces coelicolor). The name prodigiosin is derived from prodigious (i.e. something marvelous). Prodigiosin is a ...
... is obtained from a strain of streptomyces bacteria growing in soil i.e., Streptomyces pimprina. This compound is being ...
Streptomyces sp. K01-0509 produces several derivatives named guadinomines A, B, C1, C2, D, and guadinomic acid. Iwatsuki, ... Guadinomines are anti-infective compounds produced by Streptomyces sp. K01-0509. Guadinomine B is the most potent known ... Produced by Streptomyces sp. K01-0509". The Journal of Antibiotics. 61 (4): 222-229. doi:10.1038/ja.2008.32. PMID 18503201. ...
They are also found in Actinomycetes, for example Streptomyces coelicolor and some marine bacteria, including Hahella ... Streptomyces coelicolor). The group is named after prodigiosin (prodiginine) and is biosynthesized through a common set of ...
Some of the genera known to produce phenazines include Pseudomonas spp., Streptomyces spp., and Pantoea agglomerans. These ...
From 2004-2011, bafilomycins F-K were isolated from other Streptomyces sp. As one of the first identified and most commonly ... Bafilomycin A1, B1 and C1 were first isolated from Streptomyces griseus in 1983. During a screen seeking to identify microbial ... "Streptomyces lohii". The Journal of Biological Chemistry. 292 (17): 7095-7104. doi:10.1074/jbc.M116.751255. PMC 5409476. PMID ... 29-hydroxy-bafilomycin D and a number of other bafilomycins were identified from the endophytic microorganism Streptomyces sp. ...
Streptomyces coelicolor, a soil-dwelling filamentous bacterium used to produce many clinically useful antibiotics. ... doi:10.1038/s41467-020-17348-5. "Streptomyces coelicolor". John Innes Center. Retrieved 13 April 2018. "Chlamydomonas ...
Streptomyces). Bacteria generally form distinctive cell morphologies when examined by light microscopy and distinct colony ...
The bacteria Streptomyces lydicamycinicus and Streptomyces platensis produces lydicamycin. "Lydicamycin (CAS 133352-27-9)". www ... Komaki, Hisayuki; Hosoyama, Akira; Igarashi, Yasuhiro; Tamura, Tomohiko (6 March 2020). "Streptomyces lydicamycinicus sp. nov. ... "Draft genome sequence of marine-derived Streptomyces sp. TP-A0598, a producer of anti-MRSA antibiotic lydicamycins". Standards ... Novel Lydicamycin Congeners with Anti-MRSA Activity from Streptomyces platensis TP-A0598". The Journal of Antibiotics. 55 (10 ...
"Streptomyces africanus sp. nov., a novel streptomycete with blue aerial mycelium". International Journal of Systematic and ...
Streptomyces alkalithermotolerans is an alkaliphilic and thermotolerant bacterium species from the genus of Streptomyces which ... 349-359, May 2013 "Handbook of Mineralogy" (PDF). "Streptomyces file 1". www.bacterio.net. Archived from the original on 23 ... Sultanpuram, V. R.; Mothe, T; Mohammed, F (2015). "Streptomyces alkalithermotolerans sp. nov., a novel alkaliphilic and ...
Antibiotics secreting Streptomyces are likely to be found. Green algae - especially Chlorella sp. - are present all over the ... Actinomycetes (Streptomyces sp., Micromonospora sp.) are also worth mentioning. Micromonospora heviziensis for instance, a very ...
... proposed that Streptomyces avermitilis be renamed Streptomyces avermectinius. A commonly used therapy in recent times has been ... Takahashi, Y. (2002). "Streptomyces avermectinius sp. nov., an avermectin-producing strain". International Journal of ... These naturally occurring compounds are generated as fermentation products by Streptomyces avermitilis, a soil actinomycete. ... and named Streptomyces avermitilis (with the adjective probably intended to mean that it kills worms). In 2002, Yoko Takahashi ...
"Streptomyces aridus sp. nov., isolated from a high altitude Atacama Desert soil and emended description of Streptomyces ... Micromonospora inaquosa and Streptomyces aridus which were first isolated at the Purico complex. Some of these yield ...
Streptomyces albiflavescens: Streptomyces isolated from soil from rainforest areas in the Yunnan Province in the south west of ... Streptomyces alkalithermotolerans: Streptomyces which has been isolated from the Lonar soda lake in India. Anterhynchium ... Sultanpuram, V. R.; Mothe, T; Mohammed, F (2015). "Streptomyces alkalithermotolerans sp. nov., a novel alkaliphilic and ... La cập nhật 24 March 2015 Streptomyces file 1 Archived 23 September 2015 at the Wayback Machine LPSN bacterio.net Streptomyces ...
A specific type of bacteria Streptomyces camerooniansis was found to be beneficial for T. cacao by helping plant growth by ... "Streptomyces cameroonensis sp. nov., a Geldanamycin Producer That Promotes Theobroma cacao Growth". Microbes and Environments. ...
Streptomyces and other actinobacteria are major contributors to biological buffering of soils. They are also the source of many ... Actinobacteria, especially Streptomyces spp., are recognized as the producers of many bioactive metabolites that are useful to ... While many of these cause disease in humans, Streptomyces is notable as a source of antibiotics.[citation needed] Of those ... Actinobacteria is one of the dominant bacterial phyla and contains one of the largest of bacterial genera, Streptomyces. ...
Streptomyces albulus is most often used in scientific studies and is also used for the commercial production of ε-polylysine. α ... Production of polylysine by natural fermentation is only observed in strains of bacteria in the genus Streptomyces. ... Shima, S. and Sakai H. (1977). "Polylysine produced by Streptomyces". Agricultural and Biological Chemistry. 41 (9): 1807-1809 ...
Kusaka T, Yamamoto H, Shibata M, Muroi M, Kishi T, Mizuno K (1968). "Streptomyces citricolor nov. sp. and a new antibiotic, ... was isolated as a metabolite of Streptomyces citricolor. 1981: Isolation of the neplanocin family of carbocyclic nucleosides ...
August 2014). "Mullinamides A and B, new cyclopeptides produced by the Ruth Mullins coal mine fire isolate Streptomyces sp. RM- ... Procópio RE, Silva IR, Martins MK, Azevedo JL, Araújo JM (2012). "Antibiotics produced by Streptomyces". The Brazilian Journal ... January 2014). "Ruthmycin, a new tetracyclic polyketide from Streptomyces sp. RM-4-15". Organic Letters. 16 (2): 456-9. doi: ... Botulinum toxin (from Clostridium botulinum) and bleomycin (from Streptomyces verticillus) are two examples. Botulinum, the ...
Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE (March 2011). "Genome mining in Streptomyces. Elucidation of the role of Baeyer- ...
Borgia P, Campbell LL (December 1974). "Properties of two homologous alkaline proteases from Streptomyces rectus". Journal of ... Thermitase (EC, thermophilic Streptomyces serine proteinase, Thermoactinomyces vulgaris serine proteinase) is an ... Mizusawa K, Yoshida F (November 1972). "Thermophilic Streptomyces alkaline proteinase. I. Isolation, crystallization, and ...
Geldanamycin is produced by Streptomyces hygroscopicus. Erythromycin is produced by what was called Streptomyces erythreus and ... Streptomycin is produced by Streptomyces griseus. Tetracycline is produced by Streptomyces aureofaciens Vancomycin is produced ... "Antibiotics produced by Streptomyces". The Brazilian Journal of Infectious Diseases. 16 (5): 466-471. doi:10.1016/j.bjid. ... "Production of Tetracycline by Streptomyces aureofaciens in Synthetic Media". Applied Microbiology. 8 (1): 46-51. doi:10.1128/ ...
... derived from a Streptomyces sp. have micromolar affinity for LXR-α. LXR-623 (WAY 252623) CAS: [875787-07-8]. Target genes of ... "Anthrabenzoxocinones from Streptomyces sp. as liver X receptor ligands and antibacterial agents". J. Nat. Prod. 68 (9): 1437-40 ...
Cosmid cloning experiments from the Blasticidin S producer Streptomyces griseochromogenes, followed by evaluation of the ... Wagman G. H., Elsevier R. C.; p. 191 (1988). Taxonomy of Streptomyces griseochromogenes. ... "The Blasticidin S Biosynthesis Gene Cluster from Streptomyces griseochromogenes: Sequence Analysis, Organization, and Initial ... putative biosynthetic gene cluster via heterologous reconstitution of Blasticidin S production in Streptomyces lividans, ...
... which is isolated from the bacteria Streptomyces exfoliatus and Streptomyces arenae. Zhu D, Seo MJ, Ikeda H, Cane DE (February ... 2011). "Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final ...
Procópio RE, Silva IR, Martins MK, Azevedo JL, Araújo JM (2012). "Antibiotics produced by Streptomyces". The Brazilian Journal ... The aminoglycoside antibiotic streptomycin, for example, was discovered from the soil bacterium Streptomyces griseus, the ... These include the anticancer drug bleomycin (obtained from the soil bacterium Streptomyces verticillus), the immunosuppressant ... discovered from the soil bacterium Streptomyces nodosus), the antimalarial drug artemisinin (discovered from the plant ...
... was isolated from Streptomyces nodosus in 1955 and came into medical use in 1958. It is on the World Health ... It was originally extracted from Streptomyces nodosus, a filamentous bacterium, in 1955, at the Squibb Institute for Medical ... Procópio RE, Silva IR, Martins MK, Azevedo JL, Araújo JM (2012). "Antibiotics produced by Streptomyces". The Brazilian Journal ... "Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes". Chemistry ...
Examples follow: Two Streptomyces sp. Isolates, designated DAUFPE 11470 and DAUFPE 14632, isolated from corn rhizosphere soil, ... "Biological Control of Stenocarpella maydis in Maize Seed with Antagonistic Streptomyces sp. Isolates". Journal of ...
Streptomyces coelicolor and Streptomyces longisporus. The biosynthesis of undecylprodigiosin starts with PCP apoprotein which ... A prodigiosin analogue from Streptomyces". Chemical Communications (London) (22): 825-826. doi:10.1039/C19660000825. Stankovic ...
Streptomyces alboniger) Streptomycin (Streptomyces griseus) Tetracycline (Streptomyces rimosus and Streptomyces aureofaciens) ... Streptomyces venezuelae) Daptomycin (Streptomyces roseosporus) Fosfomycin (Streptomyces fradiae) Lincomycin (Streptomyces ... Nystatin (Streptomyces noursei), amphotericin B (Streptomyces nodosus), and natamycin (Streptomyces natalensis) are antifungals ... Streptomyces coelicolor, Streptomyces avermitilis, Streptomyces griseus, and Saccharopolyspora erythraea, are capable of ...
Streptomyces.org.uk homepage (John Innes Centre) StrepDB - the Streptomyces genomes annotation browser Streptomyces Genome ... ISBN 978-1-904455-77-6. Current research on Streptomyces coelicolor at the Norwich Research Park Some current Streptomyces ... Saccharopolyspora erythraea was formerly placed in this genus (as Streptomyces erythraeus). The genus Streptomyces includes ... Streptomyces is the largest genus of Actinobacteria and the type genus of the family Streptomycetaceae. Over 500 species of ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
... including Streptomyces rimosus (18), Streptomyces lividans (29), Streptomyces erythreus (now called Saccharopolyspora erythrea ... 15),Streptomyces venezuelae (17), and Streptomyces ambofaciens (66).. Keywords. Terminal Inverted Repeat Linear Plasmid Plasmid ... Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces ... Alikhanian, S. I., and Mindlin, S. Z., 1957, Recombinations in Streptomyces rimosus, Nature 180: 1208-1209.PubMedCrossRefGoogle ...
Streptomyces dengpaensis is a bacterium species from the genus of Streptomyces which has been isolated from desert soil from ... List of Streptomyces species "Species: Streptomyces dengpaensis". LPSN.DSMZ.de. Li, Yamei; Li, Yumei; Wang, Long wei; Bao, Jie ... 1 October 2018). "Streptomyces dengpaensis sp. nov., an actinomycete isolated from desert soil". International Journal of ... "Taxonomy of the species Streptomyces dengpaensis Li et al. 2018". doi:10.1601/tx.33019. Cite journal requires ,journal= (help) ...
"Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the ... Streptomyces haliclonae is a bacterium species from the genus of Streptomyces which has been isolated from the sponge Haliclona ... nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp" (PDF). ... List of Streptomyces species LPSN bacterio.net Straininfo of Streptomyces haliclonae Deutsche Sammlung von Mikroorganismen und ...
Streptomyces flavotricini is a bacterium species from the genus of Streptomyces which has been isolated from mountain forest ... Streptomyces flavotricini produces bafilomycin K and aminoacylase. List of Streptomyces species LPSN bacterio.net Straininfo of ... ISBN 978-81-322-1287-4.CS1 maint: extra text: authors list (link) Type strain of Streptomyces flavotricini at BacDive - the ... a new antifungal macrolide from Streptomyces flavotricini Y12-26". The Journal of Antibiotics. 64 (5): 391-3. doi:10.1038/ja. ...
KorSA (plasmid) [Streptomyces ambofaciens] KorSA (plasmid) [Streptomyces ambofaciens]. gi,298043,emb,CAA79637.1, ...
... (ex Ryabova and Preobrazhenskaya) Terekhova 1986. ›VKM Ac-1221. ›culture-collection:AS:4.1596. › ...
Molecular Genetic Investigation of Streptomyces Bambergiensis and Streptomyces Fradiae Strains, the Producers of Flavomycin and ... Gene Expression during Sporulation in Streptomyces Coelicolor A3(2) Keith F. Chater, Celia J. Bruton, Nigel K. Davis, Kitty A. ... Measurement of Translational Accuracy in Streptomyces J. Weiser, J.-L. Pernodet, M. Cassan, M. Ehrenberg, J. Náprstek, M. ... Molecular Genetics of Oxytetracycline Production by Streptomyces rimosus Kenneth J. McDowall, Deirdre Doyle, Michael J. Butler ...
Definition of Streptomyces gibsonii. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and ...
Streptomyces coelicolor. Streptomyces coelicolor A3(2) is the model representative of a group of soil-dwelling organisma with a ... Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2).. Bentley SD, Chater KF, Cerdeño-Tárraga AM, ... The Sanger Institute completed the sequencing of the Streptomyces coelicolor A3(2) genome in collaboration with Prof. David ...
tr,Q83WS2,Q83WS2_9ACTN Tyrosinase OS=Streptomyces castaneoglobisporus OX=79261 PE=1 SV=1 ...
... Sergey Zotchev sergey.zotchev at chembio.ntnu.no Tue Aug 29 10:18:17 EST 2000 *Previous ... which can be used for genetic manipulation of Streptomyces bacteria. Thanks in advance, Sergey Sergey Zotchev Department of ...
Streptomyces coelicolor. The Streptomyces research group is seeking knowledge of Streptomyces genetics, molecular biology and ... Streptomyces that produce antibiotics are generally found in soil. This does not mean that Streptomyces was not present in the ... CWC Streptomyces Lab. Yu T-W, Chen CW (1993) The unstable melC operon of Streptomyces antibioticus is co-deleted with a Tn4811- ... Streptomyces. The genome of Streptomyces coelicolor has recently been fully sequenced at the Sanger centre in the UK, Nature ...
Streptomyces aureofaciens ATCC ® 10762™ Designation: NRRL 2209 TypeStrain=True Application: Produces bromide peroxidase ... Streptomyces aureofaciens Duggar (ATCC® 10762™) Strain Designations: NRRL 2209 [5, ATCC 23884, Boots 876, CBS 664.68, ETH 14302 ... Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from ... Molecular cloning and sequencing of a non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762. J. Gen. ...
Streptomyces purpurogeneiscleroticus ATCC ® 19348™ Designation: HACC-186 TypeStrain=True Application: Produces lipases active ...
Genus: Streptomyces Species: Streptomyces thermoautotrophicus Name[edit]. *Streptomyces thermoautotrophicus Gadkari, Schricker ... Streptomyces thermoautotrophicus sp. nov., a thermophilic CO- and H,-oxidizing obligate chemolithoautotroph. Applied and ... Retrieved from "https://species.wikimedia.org/w/index.php?title=Streptomyces_thermoautotrophicus&oldid=5708867" ...
Streptomyces scabies synonyms, Streptomyces scabies pronunciation, Streptomyces scabies translation, English dictionary ... Noun 1. Streptomyces scabies - cause of a potato disease characterized by brownish corky tissue potato scab bacteria ... Streptomyces scabies. Also found in: Thesaurus, Wikipedia.. Related to Streptomyces scabies: Sarcoptes scabiei, Rhizoctonia ... IAA production by Streptomyces scabies and its role in plant microbe interaction.. Antagonistic Streptomyces-PR87 application, ...
Isolation of Streptomyces sp The soil samples were collected from various agricultural areas which had been serially diluted up ... The MIC value of the Streptomyces extract was determined by broth dilution method. The Streptomyces extract was gradually ... The Streptomyces isolates were grown in starch casein broth at 28ºC for 10 days and the activity were observed by well ... Isolation of Streptomyces sp. The soil samples were collected from various agricultural areas which had been serially diluted ...
... compounds are produced by the fermentation of a nutrient medium with the previously undescribed microorganism Streptomyces ... On this basis, it was designated Streptomyces avermitilis.. The above description is illustrative of a strain of Streptomyces ... Strain of streptomyces for producing avermectins and processes therewith. US5387509 *. 26 Jun 1989. 7 Feb 1995. Pfizer Inc.. ... Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins. US7585887. 29 Oct 2001. 8 Sep 2009. Bayer Cropscience ...
Bacterial dynamins stabilize FtsZ in Streptomyces. Susan Schlimpert, Sebastian Wasserstrom, Govind Chandra, Maureen J. Bibb, ... Bacterial dynamins stabilize FtsZ in Streptomyces. Susan Schlimpert, Sebastian Wasserstrom, Govind Chandra, Maureen J. Bibb, ... Streptomyces. The active organization and remodeling of cellular membranes is a fundamental process for all organisms. Many of ... 4 D and E). Furthermore, 92% of available Streptomyces genomes (n = 139) carry two dynamins and at least two sepF genes, ...
Biosynthesis of streptomyces griseus phage deoxyribonucleic acid by Judith Ann Shearer; 1 edition; First published in 1968; ... Biosynthesis of streptomyces griseus phage deoxyribonucleic acid by Judith Ann Shearer. Published 1968 . Written in English. ... You could add Biosynthesis of streptomyces griseus phage deoxyribonucleic acid to a list if you log in. ... Are you sure you want to remove Biosynthesis of streptomyces griseus phage deoxyribonucleic acid from your list? ...
Streptomyces clavuligerus. Mutation(s): 0 Gene Names: pah. EC: (PDB Primary Data), (UniProt). ... Crystal structures of PAH from Streptomyces clavuligerus (at 1.75 A and 2.45 A resolution, where 1 A=0.1 nm) imply how it binds ...
Proper noun Streptomyces hygroscopicus m 1. A taxonomic species within the genus Streptomyces - an organism whose cultures ... streptomyces-hygroscopicus. Proper noun Streptomyces hygroscopicus m. *A taxonomic species within the genus Streptomyces - an ... How would you define streptomyces-hygroscopicus? Add your definition here.. Please enable JavaScript to view the comments ...
Proteasome - Streptomyces violaceusniger [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , ...
Streptomyces species are unusual among bacteria in their extensive colony differentiation, which involves the development of ... Multilevel regulation of Streptomyces differentiation Trends Genet. 1989 Nov;5(11):372-7. doi: 10.1016/0168-9525(89)90172-8. ... Streptomyces species are unusual among bacteria in their extensive colony differentiation, which involves the development of ...
  • Streptomyces coelicolor has shown useful for the heterologous expression of proteins. (wikipedia.org)
  • Plasmids were first clearly implicated in this conjugative process in the most studied strain, Streptomyces coelicolor A3(2), when certain derivatives of the wild-type isolate were found to differ in their "fertility" properties-that is, in the frequency with which they generated chromosomal recombinants when mated with various other derivatives-and this ability was inherited "infectiously" (2, 28, 72). (springer.com)
  • Bibb, M. J., and Hopwood, D. A., 1981, Genetic studies of the fertility plasmid SCP2 and its SCP2' variants in Streptomyces coelicolor A3(2), J. Gen. Microbiol . (springer.com)
  • Streptomyces coelicolor A3(2) is the model representative of a group of soil-dwelling organisma with a complex lifecycle involving mycelial growth and spore formation. (sanger.ac.uk)
  • The Sanger Institute completed the sequencing of the Streptomyces coelicolor A3(2) genome in collaboration with Prof. David Hopwood of the John Innes Centre. (sanger.ac.uk)
  • Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). (sanger.ac.uk)
  • Wildermuth H, Wehrli E, Horne RW: The surface structure of spores and aerial mycelium in Streptomyces coelicolor. (factbites.com)
  • The X-ray structure of the two-domain laccase (small laccase) from Streptomyces coelicolor A3(2) was solved at 2.7-A resolution. (rcsb.org)
  • Streptomyces coelicolor , a filamentous, high G-C, gram-positive bacteria, was first dubbed Streptothrix coelicolor in 1908 by R. Muller after he found it on a potato(2). (kenyon.edu)
  • Later, it became known as Streptomyces coelicolor . (kenyon.edu)
  • The Streptomyces coelicolor A3(2) strain studied in depth by David A Hopwood and sequenced by the John Innes Center and the Sanger Institute is actually taxonomically a member of the Streptomyces violaceoruber genus, although it retains the former name, and is not the same strain as the Muller strain(25). (kenyon.edu)
  • Streptomyces coelicolor , like the streptomyces genus in general, live in the soil. (kenyon.edu)
  • Colonies of Streptomyces coelicolor release pigments that are blue/green in alkali and red in acidic conditions, thereby giving the bacterial colonies those colors under the respective conditions. (kenyon.edu)
  • Other differentiating characteristics of Muller's Streptomyces coelicolor are grayish-yellow aerial mycelium, smooth spores, aerial mycelium lacking spirals, and no melanoid pigment(5). (kenyon.edu)
  • From this point on, I will refer to Streptomyces coelicolor as the A3(2) strain and not Muller's strain because the A3(2) strain was sequenced, and a great deal of information is available about it. (kenyon.edu)
  • Streptomyces coelicolor are important bacteria and were sequenced because of their "adaptability to environmental stress", "source of bioactive molecules for medicine and industry", and "relat[ion] to human pathogens"(3). (kenyon.edu)
  • Streptomyces coelicolor has a very similar core genome to Mycobacterium tuberculosis and Corynebacterium diphtheriae , as well as some similarity to Mycobacterium leprae , so it can be used to study these disease causing bacteria(4). (kenyon.edu)
  • Streptomyces coelicolor also has an interesting life-cycle that includes differentiation into aerial mycelium and spore formation(3). (kenyon.edu)
  • Streptomyces coelicolor has one linear chromosome and two plasmids, one that is linear and one that is circular. (kenyon.edu)
  • Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. (frontiersin.org)
  • Streptomyces coelicolor is considered the model organism among Gram positive, GC rich bacteria. (nih.gov)
  • To further characterize the molecular basis of these developmental events, we screened for mutants of Streptomyces coelicolor that exhibit aberrant morphological differentiation and/or secondary metabolite production. (asm.org)
  • As a means of understanding the pathways responsible for differentiation in this important bacterial genus, over the past 30 years several screening analyses for developmental mutants have been carried out with the model species Streptomyces coelicolor ( 6 , 15 , 26 , 32 , 37 ). (asm.org)
  • Streptomyces coelicolor contained an innate Tc-controllable promoter regulated by a TetR homo-logue (SCO0253). (psu.edu)
  • As an initial step toward understanding these chemical interactions at a systems level, we employed the emerging techniques of nanospray desorption electrospray ionization (NanoDESI) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) imaging mass spectrometry to gain a global chemical view of the model bacterium Streptomyces coelicolor interacting with five other actinomycetes. (asm.org)
  • Most actinomycetes dedicate a substantial fraction of their genomes to the production of such natural products (~4.5% in the case of Streptomyces coelicolor ) ( 3 , 5 ). (asm.org)
  • Background: The transition from exponential to stationary phase in Streptomyces coelicolor is accompanied by a major metabolic switch and results in a strong activation of secondary metabolism. (rug.nl)
  • Our main research interest is studying bacterial development of Streptomyces coelicolor, a Gram-positive, filamentous bacterium. (openwetware.org)
  • Unlike most bacteria that divide by binary fission, in Streptomyces coelicolor long, multigenomic filaments are formed with ocasional septa and regular branching. (openwetware.org)
  • Streptomyces coelicolor , a non-pathogenic soil-dwelling organism, is thus used as a model for the study of intrinsic resistance. (rsc.org)
  • Single colonies and soil cultures of Streptomyces antibioticus ATCC 11891 and Streptomyces coelicolor M145 were used for this analysis. (asm.org)
  • Davide d'Alia has predicted, by computational analysis, a large number of ncRNAs especially overlapping with protein-coding genes, which are not commonly found in bacteria, in the antibiotic-producing soil bacterium Streptomyces coelicolor . (rug.nl)
  • Here, we have assessed the ability of Streptomyces coelicolor, Streptomyces griseus, Streptomyces albus, Streptomyces antibioticus and Streptomyces champavatii to provide disease protection against Rhizoctonia solani in Solanum lycopersicon and have also examined associated changes in hydrogen peroxide (H2O2) production, lipid peroxidation (LPO) and antioxidant enzymes. (ovid.com)
  • SC , the Streptomyces coelicolor homologue of the Bacillus subtilis protein DivIVA, is essential and directly involved in hyphal tip growth and morphogenesis. (lu.se)
  • in Streptomyces coelicolor, essentially all of its 140 histidine kinases appear to be ancient or derived from lineage-specific expansions. (harvard.edu)
  • Streptomyces antibiotics include: Chloramphenicol (Streptomyces venezuelae) Daptomycin (Streptomyces roseosporus) Fosfomycin (Streptomyces fradiae) Lincomycin (Streptomyces lincolnensis) Neomycin (Streptomyces fradiae) Platensimycin (Streptomyces platensis) Puromycin (Streptomyces alboniger) Streptomycin (Streptomyces griseus) Tetracycline (Streptomyces rimosus and Streptomyces aureofaciens) Clavulanic acid (Streptomyces clavuligerus) is used in combination with some antibiotics (like amoxicillin) to weaken bacterial-resistance. (wikipedia.org)
  • Cloning and analysis of the telomere and terminal inverted repeat of the linear chromosome of Streptomyces griseus. (factbites.com)
  • You could add Biosynthesis of streptomyces griseus phage deoxyribonucleic acid to a list if you log in . (openlibrary.org)
  • Are you sure you want to remove Biosynthesis of streptomyces griseus phage deoxyribonucleic acid from your list? (openlibrary.org)
  • The production of streptomycin using Streptomyces griseus using two types of chitin as a substrate was studied using a variety of fermentation techniques. (bl.uk)
  • In this study, the co-linearity inherent to polyketide pathways was used to identify the zincophorin biosynthesis gene cluster in the genome of the natural producer Streptomyces griseus HKI 0741. (egms.de)
  • The influence of Streptomyces avermitilis, S. griseus, and S. orientalis on germination of surface-disinfested Gigaspora margarita, Glomus mosseae, and Scutellispora heterogama spores on 1.5% Noble water agar (WA) was studied. (apsnet.org)
  • Germination of G. mosseae spores was also stimulated by S. avermitilis and S. griseus on double-layer WA but was generally poor on WA not containing Streptomyces microcolonies. (apsnet.org)
  • Biotransformation of 7-ethoxycoumarin by Streptomyces griseus resulted in the accumulation of two metabolites which were isolated and identified as 7-hydroxycoumarin and 7-hydroxy-6-methoxycoumarin. (asm.org)
  • This value was very close to that measured with the technique of proteases from Streptomyces griseus (74.6% CP), but significantly lower (P≤0.05) than the one obtained by the in situ methodology (84.8% CP). (scielo.cl)
  • How many antibiotics are produced by the genus Streptomyces? (wikipedia.org)
  • Streptomyces is the largest genus of Actinobacteria and the type genus of the family Streptomycetaceae. (wikipedia.org)
  • Streptomyces is the type genus of the family Streptomycetaceae and currently covers close to 576 species with the number increasing every year. (wikipedia.org)
  • Saccharopolyspora erythraea was formerly placed in this genus (as Streptomyces erythraeus). (wikipedia.org)
  • The genus Streptomyces includes aerobic, Gram-positive, filamentous bacteria that produce well-developed vegetative hyphae (between 0.5-2.0 µm in diameter) with branches. (wikipedia.org)
  • So far, ten species belonging to this genus have been found to be pathogenic to plants: S. scabiei S. acidiscabies S. europaeiscabiei S. luridiscabiei S. niveiscabiei S. puniciscabiei S. reticuliscabiei S. stelliscabiei S. turgidiscabies (scab disease in potatoes) S. ipomoeae (soft rot disease in sweet potatoes) Streptomyces is the largest antibiotic-producing genus, producing antibacterial, antifungal, and antiparasitic drugs, and also a wide range of other bioactive compounds, such as immunosuppressants. (wikipedia.org)
  • Streptomyces dengpaensis is a bacterium species from the genus of Streptomyces which has been isolated from desert soil from the Dengpa District in Tibet. (wikipedia.org)
  • Streptomyces haliclonae is a bacterium species from the genus of Streptomyces which has been isolated from the sponge Haliclona sp in the Chiba prefecture from the coast of Tateyama City in Japan. (wikipedia.org)
  • Streptomyces flavotricini is a bacterium species from the genus of Streptomyces which has been isolated from mountain forest soil. (wikipedia.org)
  • Several species of Streptomyces are involved in a symbiotic relationship with species of ants in the genus Attini. (factbites.com)
  • The small bacterium in the streptomyces genus inhabits the cuticles of the ants, and aids in weeding their fungal gardens. (factbites.com)
  • Streptomyces , bacterial genus of the order Actinomycetales , members of which resemble fungi in their branching filamentous structure. (factbites.com)
  • A taxonomic species within the genus Streptomyces - an organism whose cultures produce useful chemical agents. (yourdictionary.com)
  • The Streptomyces genus is responsible for producing a majority of the antibiotics in use today, as well as some immunosuppressants and anti-tumor agents. (kenyon.edu)
  • Notably, Streptomyces accounts for 39% of all microbial metabolites, and in Streptomycetales class, this genus alone reported to produce nearly 80% of bioactive molecules [ 1 ]. (hindawi.com)
  • Until recently, most of the compounds from genus Streptomyces have been isolated by culture-dependent methods rather than by the metagenomic approach. (hindawi.com)
  • As evidenced above, Streptomyces is an undoubtedly potent genus to hunt for novel pharmaceutically essential compounds derived from underexplored extreme environment habitats for next-generation drugs to counteract the worldwide increase of drug resistance and to meet the demand for novel drugs with no or fewer side effects. (hindawi.com)
  • Filamentous soil bacteria of the genus Streptomyces carry out complex developmental cycles that result in sporulation and production of numerous secondary metabolites with pharmaceutically important activities. (asm.org)
  • The major interest in this genus Streptomyces is the diversity of its production of secondary metabolites as an industrial microorganism. (pnas.org)
  • Bacteria in the genus Streptomyces are major produ-cers of antibiotics and other pharmacologically active compounds. (psu.edu)
  • Gram-positive soil bacteria included in the genus Streptomyces produce a large variety of secondary metabolites in addition to extracellular hydrolytic enzymes. (mdpi.com)
  • Here we show that in Streptomyces , a genus of Actinobacteria abundant in soil and symbiotic niches, the ability to rapidly degrade cellulose is largely restricted to two clades of host-associated strains and is not a conserved characteristic of the Streptomyces genus or host-associated strains. (osti.gov)
  • Background]: Bacteria included in the genus Streptomyces exhibit several attractive characteristics that make them adequate hosts for the heterologous expression of proteins. (csic.es)
  • Within the polymicrobial communities occupying the soil, Streptomyces represent the largest genus of the ubiquitous actinomycetes group. (elifesciences.org)
  • Among Actinomycetes, Streptomyces is the most studied and best known genus. (tu-darmstadt.de)
  • Within the genus, there are members like Streptomyces mobaraensis exporting transglutaminase (TGase). (tu-darmstadt.de)
  • Over 500 species of Streptomyces bacteria have been described. (wikipedia.org)
  • In recent years, biotechnology researchers have begun using Streptomyces species for heterologous expression of proteins. (wikipedia.org)
  • Though E. coli strains have secretion mechanisms, these are of low efficiency and result in secretion into the periplasmic space, whereas secretion by a Gram-positive bacterium such as a Streptomyces species results in secretion directly into the extracellular medium. (wikipedia.org)
  • In addition, Streptomyces species have more efficient secretion mechanisms than E.coli. (wikipedia.org)
  • Taxonomy of the species Streptomyces dengpaensis Li et al. (wikipedia.org)
  • Few species of Streptomyces are pathogenic for animals, although a few species cause plant diseases. (factbites.com)
  • Streptomyces species produce spores from aerial filaments called sporophores. (factbites.com)
  • Streptomyces species are found worldwide in soil and are important in soil ecology. (factbites.com)
  • Indeed, one of the surprises brought by the genome sequencing of Streptomyces was the fact that the best-studied species contain a number of cryptic genes clusters involved in secondary metabolism. (factbites.com)
  • Streptomyces species are unusual among bacteria in their extensive colony differentiation, which involves the development of spore-bearing aerial hyphae on mycelial colonies. (nih.gov)
  • Considering this, in recent years, much of the attention focused on more extreme environment habitats such as deep-sea, desert, cryo, and volcanic environments for the isolation of potential Streptomyces species. (hindawi.com)
  • Unique among bacteria, Streptomyces has a very complex life cycle, and a linear chromosome instead of the usual circular chromosome found in most bacterial species. (jic.ac.uk)
  • It is now clear that this is also true for most Streptomyces species. (jic.ac.uk)
  • the majority of antibiotics in current use, as well as drugs with anticancer, antifungal, and immunosuppressant activities, are obtained from Streptomyces species ( 5 ). (asm.org)
  • The synthetic Tc controllable promoter (tcp), tcp830, was active in a wide range of Streptomyces species, and varying levels of induction were observed after the addition of 1-100 ng/ml of anhydrotetracycline (aTc). (psu.edu)
  • Alternatively, land can be managed by providing nutrients to encourage the growth of specific species, like Streptomyces , within a given plot, but this takes longer to develop. (asm.org)
  • Streptomyces species belong to the Actinomycetes group and are bacteria that share many characteristics with the fungi. (sciencephoto.com)
  • The alkaloid SB-203207 ( 3 ), isolated from a Streptomyces species by a SmithKline Beecham group, was shown to inhibit isoleucyl t RNA synthetase with an IC 50 of less than 2 nM. (organic-chemistry.org)
  • Although Streptomyces species express multiple catalase/peroxidase and organic hydroperoxide resistance genes to protect them against peroxide damage, a wild type strain of S. lividans exhibited two-fold to 10-fold higher survival, compared to a dnd − mutant, following treatment with peroxides. (frontiersin.org)
  • Originally, the modification was found in the soil-inhabiting, antibiotic-producing Streptomyces species. (frontiersin.org)
  • Your task will be to study the role of these Streptomyces secondary metabolites in soil microbial communities , by first identifying and characterize the biosynthetic pathways required for the biosynthesis of these compounds using our genome mining and CRISPR-based genome editing technology and then studying the effect of the engineered (knock-out) strains on the species diversity and functionality of soil mock-microbial communities. (dtu.dk)
  • Method for characterizing Streptomyces species. (currentprotocols.com)
  • Specifically, when a species of Streptomyces is grown with yeast, some of the bacteria start to explore their environment, move over top of other bacteria and up hard surfaces to heights that would be the equivalent of humans scaling Mount Everest. (elifesciences.org)
  • The ability of Streptomyces species to act as biocontrol agents for plant pathogens via induced systemic resistance has been demonstrated and considerable efforts have been made in elucidating the underlying mechanisms of Streptomyces-host plant-phytopathogen interactions. (ovid.com)
  • This study offers novel insights into the mechanisms of priming by Streptomyces and highlights their capacity to activate plant defence responses generated by biotic stress through induction of antioxidant enzymes along with improved reactive oxygen species management. (ovid.com)
  • Our comparative genomic analyses revealed that each of the eleven Streptomyces species examined possesses a rich repertoire of from 761-1258 transport proteins, accounting for 10.2 to 13.7 % of each respective proteome. (biomedcentral.com)
  • The evolutionary flexibility across eleven Streptomyces species is seen in the lineage-specific distribution of transport proteins in two major protein translocation pathways: the general secretory (Sec) pathway and the twin-arginine translocation (Tat) pathway. (biomedcentral.com)
  • Our results present a catalog of transport systems in eleven Streptomyces species. (biomedcentral.com)
  • Non-Streptomyces actinomycetes, filamentous fungi, and non-filamentous bacteria, have also yielded important antibiotics. (wikipedia.org)
  • The bio-oil blocked the growth of the bacteria Streptomyces scabies and Clavibacter michiganensis and the fungus Pythium ultimum. (thefreedictionary.com)
  • During sporulation, the filamentous bacteria Streptomyces undergo a massive cell division event in which the synthesis of ladders of sporulation septa convert multigenomic hyphae into chains of unigenomic spores. (pnas.org)
  • Among Gram-positive bacteria, Streptomyces represents a significant source for supplying bioactive natural products with clinical and pharmaceutical applications. (hindawi.com)
  • This is the norm in eukaryotes such as yeast, human, and plant cells but has not been demonstrated in other bacteria apart from Streptomyces . (jic.ac.uk)
  • Soil from the field (and on the potatoes) contained Streptomyces isolates that showed antimicrobial activity against bacteria, fungi, nematodes, and oomycetes. (asm.org)
  • We are characterising two classes of these proteins in the filamentous bacteria Streptomyces . (openwetware.org)
  • Our results suggest that host-associated niches have selected some symbiotic Streptomyces for increased cellulose degrading activity and that symbiotic bacteria are a rich biochemical and enzymatic resource for biotechnology. (osti.gov)
  • It has long been thought that the life cycle of Streptomyces bacteria encompasses three developmental stages: vegetative hyphae, aerial hyphae and spores. (elifesciences.org)
  • These results reveal that interkingdom interactions can trigger novel developmental behaviours in bacteria, here, causing Streptomyces to deviate from its classically-defined life cycle. (elifesciences.org)
  • In fact, soil gets it characteristic 'earthy' smell from a common type of soil bacteria known as Streptomyces . (elifesciences.org)
  • Scientists have been studying Streptomyces for over 70 years, and in all this time, there has been unanimous agreement on how these bacteria grow. (elifesciences.org)
  • That is to say that, unlike most other bacteria, Streptomyces grow like plants: they don't move, and instead produce spores that are dispersed like seeds. (elifesciences.org)
  • This stationary lifestyle makes these bacteria somewhat vulnerable to predators, and so it is thought that Streptomyces make antibiotics to help protect themselves from other bacteria that are able to move around in the soil. (elifesciences.org)
  • have discovered that Streptomyces bacteria can indeed move when grown in the presence of fungi. (elifesciences.org)
  • also found that these "explorer" Steptomyces can communicate with nearby Streptomyces bacteria with a perfume-like airborne signal and convince their relatives to begin exploring too. (elifesciences.org)
  • Furthermore, while this volatile signal promotes the growth of Streptomyces , it adversely affects other bacteria and makes them sicker such that they are less able to grow and survive. (elifesciences.org)
  • Paradoxically, Streptomyces bacteria thrive in these iron-depleted niches, both rewiring their gene expression and metabolism to facilitate iron uptake and increasing their exploration rate. (asm.org)
  • Plasmids responsible for "fertility" (or "chromosome mobilizing ability" [Cma]) (24) were also identified genetically in some other strains, including Streptomyces rimosus (18), Streptomyces lividans (29), Streptomyces erythreus (now called Saccharopolyspora erythrea ) (15), Streptomyces venezuelae (17), and Streptomyces ambofaciens (66). (springer.com)
  • Lin Y-S, Kieser HM, Hopwood DA, Chen CW (1993) The chromosomal DNA of Streptomyces lividans 66 is linear. (factbites.com)
  • Lin Y-S, Chen CW (1997) Instability of artificailly circularised chromosomes of Streptomyces lividans. (factbites.com)
  • Three decades ago Professor Carton Chen's group of the National Yang-Ming University in Taiwan, and Sir David Hopwood's group at the John Innes Institute showed that the chromosome of Streptomyces lividans is linear. (jic.ac.uk)
  • The membrane of Streptomyces lividans cells lets potassium ions in but not sodium via a potassium-specific channel. (asknature.org)
  • DNA phosphorothioation, conferred by dnd genes, was originally discovered in the soil-dwelling bacterium Streptomyces lividans , and thereafter found to exist in various bacterial genera. (frontiersin.org)
  • Streptomyces lividans NCIMB 11416/IL3 p002 secreting human interleukin-3 was used as the host organism in this study of improving target protein production. (uwaterloo.ca)
  • Results]: In order to use the yefM/yoeBsl system to stabilize expression plasmids in Streptomyces, a S. lividans mutant strain that contained only the toxin gene (yoeBsl) in its genome and the antitoxin gene (yefMsl) located in a temperature-sensitive plasmid was constructed and used as host. (csic.es)
  • The gene encoding viomycin phosphotransferase has been cloned from S. vinaceus into Streptomyces lividans, in which it determines resistance to viomycin. (le.ac.uk)
  • Engineering Diverse Eubacteria Promoters for Robust Gene Expression in Streptomyces lividans. (bioportfolio.com)
  • Streptomyces is a naturally occurring bacterium in soil and is likely to be present in aquatic habitats as well [1]. (factbites.com)
  • Dr Marcin Szafran and the team employed Hi-C technique (short for chromosome conformation capture with deep sequencing) to show that the arms of the Streptomyces venezuelae chromosome are separated from each other when the bacterium is in the early stage of its life cycle. (jic.ac.uk)
  • Streptomyces avermitilis is a soil bacterium that carries out not only a complex morphological differentiation but also the production of secondary metabolites, one of which, avermectin, is commercially important in human and veterinary medicine. (pnas.org)
  • StreptomeDB, a directory of Streptomyces isolates, contains over 2400 compounds isolated from more than 1900 strains. (wikipedia.org)
  • Genetic exchange in Streptomyces was first revealed when prototrophic recombinants were recovered from mixed cultures of pairs of auxotrophic derivatives of several wild-type strains (1, 8, 25, 63, 65). (springer.com)
  • 8. The process of claim 7 wherein the C-076 producing strains is Streptomyces avermitilis NRRL 8165 (ATCC 31267). (google.co.uk)
  • The present inventors have now discovered that the fermentation of certain variant strains of Streptomyces carzinostaticus obtained by ultraviolet radiation and the single-spore isolation method produce a relatively stable substance which can be recovered in pure form. (google.es)
  • Characterization of chromosome ends of eight Streptomyces strains has revealed evidence of linear chromosomes, indicating that chromosomal linearity might be common in the streptomycetes ( 3 ). (pnas.org)
  • Our comparative genomics identify that while plant biomass degrading genes (CAZy) are widespread in Streptomyces , key enzyme families are enriched in highly cellulolytic strains. (osti.gov)
  • The TS producing organism, Streptomyces azureus, biosynthesizes thiostrepton-resistance methyltransferase (TSR), an enzyme that uses S-adenosyl-L-methionine (AdoMet) as a methyl donor, to modify the TS target site. (uwaterloo.ca)
  • In contrast, the resistance of the erythromycin-producing organism, Streptomyces erythreus. (le.ac.uk)
  • In S. venezuelae, the Streptomyces strain which was used as a model organism in this work, there are ten potentially c-di-GMP metabolizing enzymes, of which two GGDEF-EAL tandem proteins, RmdA and RmdB, are the focus of this work. (hu-berlin.de)
  • which comprises fermenting with a C-076 producing strain of Streptomyces avermitilis ATCC 31267 or ATCC 31272, an aqueous nutrient medium containing an assimilable source of carbon and an assimilable source of nitrogen and inorganic salts under aerobic conditions and recovering said C-076 compounds from said fermentation broth. (google.co.uk)
  • In particular, it is concerned with novel compounds, collectively identified as C-076, which are produced by the fermentation of a nutrient medium with a strain of the microorganism Streptomyces avermitilis. (google.co.uk)
  • Since the A3(2) strain is actually Streptomyces violaceoruber , it looks a bit different. (kenyon.edu)
  • Thus, there is provided according to the present invention the process for the production of an antibiotic designated neocarzinostatin which comprises cultivating a neocarzinostatin-producing variant strain of Streptomyces carzinostaticus designated Streptomyces carzinostazicus var. (google.es)
  • This strain of Streptomyces hygroscopicus produces the antibiotic, milbemycin, which is used as an insecticide and also to control certain parasitic infections in animals. (sciencephoto.com)
  • A new series of milbemycin antibiotics from Streptomyces strain E225. (nii.ac.jp)
  • Similarly, ethyl acetate crude extract obtained from Streptomyces strain CH54-4 was reported for best antimicrobial and anti proliferative activity (8). (thefreelibrary.com)
  • Potential Actinobacterial strain SKCMM1 was characterized morphologically and physiologically as prescribed in International Streptomyces Project (ISP) (9). (thefreelibrary.com)
  • Streptomyces isolates have yielded the majority of human, animal, and agricultural antibiotics, as well as a number of fundamental chemotherapy medicines. (wikipedia.org)
  • Streptomyces and closely related genera produce more than 50% of the 200 commercially available antibiotics and most of the 10,000 known antibiotics . (factbites.com)
  • We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces , a well-known producer of antibiotics. (frontiersin.org)
  • Expanding the use of the last line of antibiotics and demand for new drugs will continue to play an essential role for the potent Streptomyces from previously unexplored environmental sources. (hindawi.com)
  • Historically, Streptomyces from environmental sources has been pivotal in the discovery of important bioactive secondary metabolites including antibiotics, immunosuppressive drugs, anticancer drugs, and other biologically active compounds [ 3 - 6 ]. (hindawi.com)
  • The most interesting property of Streptomyces is its ability to produce secondary metabolites including antibiotics and bioactive compound ( 2 ) value in human and veterinary medicine, agriculture, and unique biochemical tools. (pnas.org)
  • Sansanmycins were a group of nucleosidyl-peptide antibiotics, produced by Streptomyces sp SS (CGMCC no. 1764). (nature.com)
  • Streptomyces kanasensis ZX01 produces some antibiotics and a glycoprotein with antiviral activity. (mdpi.com)
  • Streptomyces tenebrarius H6 mainly produces three kinds of antibiotics: apramycin, carbamoyltobramycin and some carbamoylkanamycin B. In our present study, a dehydrogenase gene tacB in the tobramycin biosynthetic gene cluster was disrupted by in-frame deletion. (nature.com)
  • This finding could be a valuable tool for using Streptomyces as a host to produce proteins at the industrial and pharmaceutical levels without the use of antibiotics in the production step. (csic.es)
  • Remarkably, Streptomyces are also the original sources of most of the antibiotics that are prescribed by doctors to treat bacterial infections. (elifesciences.org)
  • Mode of interaction between β-lactam antibiotics and the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R39. (portlandpress.com)
  • Leicester Research Archive: Resistance to antibiotics in antibiotic-producing streptomyces. (le.ac.uk)
  • Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes. (kegg.jp)
  • Six enzymes are required for the biosynthesis of dTDP-desosamine in Streptomyces venezuelae, with the last step catalyzed by DesVI, an N,N-dimethyltransferase. (osti.gov)
  • In response to this low-iron environment, Streptomyces venezuelae secretes a suite of differentially modified siderophores and upregulates genes associated with siderophore uptake. (asm.org)
  • Actinomycin was the first antibiotic isolated from Streptomyces in 1940, followed by streptomycin three years later. (wikipedia.org)
  • The antibiotic streptomycin takes its name directly from Streptomyces. (wikipedia.org)
  • The antibiotic pacidamycin ( Figure 1 ) (Streptomyces coeruleorubidus), the HIV-1 integrase inhibitor complestatin ( Figure 2 ) (Streptomyces lavendulae), and the potential antitumor agent spirotryprostatin ( Figure 3 ) (Aspergillus fumigatus) all contain a tryptophan moiety. (openwetware.org)
  • Firstly, Streptomyces vinaceus and Streptomyces capreolus appear to be resistant to their products, viomycin and capreomycin, respectively, through the possession of novel enzymes (viomycin/capreomycin phosphotransferase, capreomycin acetyltransferase) which have the capacity to maintain intracellular antibiotic in inactive forms. (le.ac.uk)
  • A better understanding of transport systems will allow enhanced optimization of production processes for both pharmaceutical and industrial applications of Streptomyces , which are widely used in antibiotic production and heterologous expression of recombinant proteins. (biomedcentral.com)
  • Virginiamycin M 1 (VM 1 ), produced by Streptomyces virginiae , is a polyunsaturated macrocyclic lactone antibiotic belonging to the virginiamycin A group. (asm.org)
  • The antibiotic virginiamycin, produced by Streptomyces virginiae , is a member of the virginiamycin family (alternatively designated as the streptogramin family or the mikamycin family) ( 2 ). (asm.org)
  • At 10.1 Mbp long and encoding 9,107 provisional genes, it is the largest known Streptomyces genome sequenced, probably due to the large pathogenicity island. (wikipedia.org)
  • From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. (frontiersin.org)
  • this is the largest bacterial genome sequence, and it provides insights into the intrinsic diversity of the production of the secondary metabolites of Streptomyces . (pnas.org)
  • Zhang, X. Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01. (mdpi.com)
  • Zhang G, Yu D, Sang B, Feng J, Han L, Zhang X. Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01. (mdpi.com)
  • Genome mining for the search and discovery of bioactive compounds: The Streptomyces paradigm. (bioportfolio.com)
  • Molecular cloning and sequencing of a non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762. (atcc.org)
  • Quinoxaline, a mutagenic azaarene produced in foods during cooking, was added to cultures of Streptomyces badius ATCC 39117. (ovid.com)
  • Streptomyces avermitilis synthesizes the antiparasitic ivermectin (Stromectol). (wikipedia.org)
  • Novel compounds are produced by the fermentation of a nutrient medium with the previously undescribed microorganism Streptomyces avermitilis. (google.co.uk)
  • Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinama K . Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773. (nature.com)
  • Landomycin biosynthesis and its regulation in Streptomyces. (bioportfolio.com)
  • Most Streptomyces chromosomal DNA molecules are about 8-Mb-long, with terminal-inverted repeats and covalently bound terminal proteins supposedly at the 5′ end. (pnas.org)
  • Among them, the ATP-binding Cassette (ABC) superfamily and the Major Facilitator Superfamily (MFS) represent more than 40 % of all the transport proteins in Streptomyces . (biomedcentral.com)
  • Linda Kinkel discusses new insights into the plant rhizosphere and the ways that some Streptomyces isolates can protect agricultural crops against bacterial, fungal, oomycete, and nematode infections. (asm.org)
  • This is the first report of a functional ncRNA in Streptomyces and also prompts a functional role for the non-coding transcripts in bacterial cells. (rug.nl)
  • The results further expand the repertoire of interspecies interactions and nutrient cues that impact Streptomyces exploration and provide new mechanistic insight into this unique mode of bacterial growth. (asm.org)
  • SC is a novel bacterial morphogene, and it is localized at or very close to the apical sites of peptidoglycan assembly in Streptomyces hyphae. (lu.se)
  • AF010134 Streptomyces aureofaciens putative sporulation protein gene, complete cds. (atcc.org)
  • U01096 Streptomyces aureofaciens bromoperoxidase BPO-A1 (bpoA1) gene, complete cds. (atcc.org)
  • L11647 Streptomyces aureofaciens glycogen branching enzyme (glgB) gene, complete cds. (atcc.org)
  • AF007125 Streptomyces aureofaciens elongation factor G (fus) gene, partial cds, and elongation factor Tu (tuf1) gene, complete cds. (atcc.org)
  • Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptomyces tenebrarius . (nature.com)
  • The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB8223: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine. (nature.com)
  • Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin Streptomyces avermitils. (nii.ac.jp)
  • Due to the lack of powerful gene regulation elements, the engineering development of Streptomyces is often limited. (bioportfolio.com)
  • Streptomyces turgidiscabies , a cause of potato scab, possesses a mobilizable pathogenicity island containing multiple virulence genes and a cytokinin biosynthetic pathway. (apsnet.org)
  • Evolutionary analyses identify complex genomic modifications that drive plant biomass deconstruction in Streptomyces , including acquisition and selective retention of CAZy genes and transcriptional regulators. (osti.gov)
  • potato is caused by the actinomycete, Streptomyces scabies (Lambert and Loria, 1989. (factbites.com)
  • IAA production by Streptomyces scabies and its role in plant microbe interaction. (thefreedictionary.com)
  • Chicken manure caused a temporary initial increase in soil pH to 8 or higher, which is at the upper limit for Streptomyces scabies to cause potato scab. (thefreedictionary.com)
  • Molecular monitoring of Gramonol herbicide biodegradation in relation to streptomyces scabies isolates. (thefreedictionary.com)
  • Taxtomina parcialmente purificada e preparações de Streptomyces scabies na indução. (usp.br)
  • Dentre os indutores de resistência, as fitotoxinas apresentam potencial como ativadoras de plantas e neste caso, mais especificamente a taxtomina, produzida por Streptomyces scabies . (usp.br)
  • O objetivo deste trabalho foi avaliar o potencial da fitotoxina taxtomina parcialmente purificada (TPP) e preparações de Streptomyces scabies como potenciais indutores de resistência em soja à Phakopsora pachyrhizi . (usp.br)
  • Foram realizados ensaios para avaliar o efeito direto dos potenciais indutores deresistência a base de suspensão de células de Streptomyces scabies , filtrado de meio de cultivo de Streptomyces scabies , TPP e acibenzolar-S-metílico sobre a germinação de urediniósporos através do teste de microgotas em placas de Petri de poliestireno. (usp.br)
  • Among the resistance inducers, phytotoxins have the potential as plant activators and in this case, more specifically thaxtomin, produced by Streptomyces scabies . (usp.br)
  • Therefore, the objective of this work was to evaluate the potential of the partially purified phytotoxin thaxtomin (TPP) and preparation of Streptomyces scabies as potential inducers of resistance in soybean to Phakopsora pachyrhizi . (usp.br)
  • Novella IS, Barbes C, Sanchez J: Sporulation of Streptomyces antibioticus ETH 7451 in submerged culture. (factbites.com)
  • Using a cell biological approach, we show that the two Streptomyces dynamins specifically localize to sporulation septa in an FtsZ-dependent manner. (pnas.org)
  • Streptomyces isolates are typically initiated with the aerial hyphal formation from the mycelium. (wikipedia.org)
  • The Streptomyces sp isolates were maintained on starch casein agar slants. (ipl.org)
  • The Streptomyces isolates were grown in starch casein broth at 28ºC for 10 days and the activity were observed by well diffusion method. (ipl.org)
  • This discovery led Neil, new University of Minnesota professor Linda, and their collaborators to study the antimicrobial activity of natural Streptomyces isolates from around the world. (asm.org)
  • Morphological differentiation in Streptomyces involves the formation of a lawn of aerial hyphae on the colony surface that stands up into the air and differentiates into chains of spores ( 1 ). (pnas.org)
  • How this unusual linear chromosome is folded in three-dimensional space, and how folding might change during the life cycle of Streptomyces is not fully understood. (jic.ac.uk)
  • To better understand the folding of the Streptomyces chromosome, researchers at the University of Wrocław (Poland) collaborated with the John Innes Centre. (jic.ac.uk)
  • The research also showed that the ends of the linear chromosome are organised into distinct domains by a unique Streptomyces protein called HupS. (jic.ac.uk)
  • Thus, the Streptomyces chromosome is unique in its structure and size. (pnas.org)
  • Characterization of L -glutamine: 2-deoxy-scyllo-inosose aminotransferase ( tbmB ) from Streptomyces tenebrarius . (nature.com)
  • Taxonomic Characterization and Antagonistic Efficacy of Streptomyces cavourensis SKCMM1 Isolated from Sediment of Pichavaram Mangrove Forest. (thefreelibrary.com)
  • In the current study, Streptomyces SKCMM1 is isolated from Pichavaram Mangrove forest of Tamil Nadu and identified through polyphasic taxonomic characterization approach. (thefreelibrary.com)
  • Linda Kinkel describes the important relationships between plants and microbes and her research on Streptomyces that protect against plant pathogens. (asm.org)
  • Further in silico prediction of antimicrobial potential of Streptomyces sp. (frontiersin.org)
  • Isolation of very low-copy plasmids from Streptomyces spp. (qiagen.com)
  • Conclusions]: This is the first report that describes the use of a toxin-antitoxin system to maintain high -copy plasmids in Streptomyces. (csic.es)
  • A recent screening of TCM extracts revealed a Streptomyces that produces a number of antitubercular pluramycins. (wikipedia.org)
  • Streptomyces flavotricini produces bafilomycin K and aminoacylase. (wikipedia.org)
  • Streptomyces also produces several proteases including extracellular endoprotease that truncate the N-terminus of the recombinant protein. (uwaterloo.ca)
  • We describe the isolation of Streptomyces bikiniensis from multiple blood cultures in a single patient over the course of 1 week, further illustrating that Streptomyces is pathogenic and a cause of bacteremia even in the absence of overt clinical symptoms and risk factors. (factbites.com)
  • the isolation of Streptomyces from repeat blood cultures strongly suggests a pathogenic role. (factbites.com)
  • 1961) Witt and Stackebrandt 1991 is a heterotypic synonym of "Streptomyces caespitosus" (NBRC 13490). (factbites.com)
  • Crystal structures of PAH from Streptomyces clavuligerus (at 1.75 A and 2.45 A resolution, where 1 A=0.1 nm) imply how it binds beta-lactams rather than the amino acid substrate of the arginases from which it evolved. (rcsb.org)
  • L22864 Streptomyces aureofaciens whiB operon, complete sequence. (atcc.org)
  • M84990 Streptomyces aureofaciens chloroperoxidase CPO-A2 (cpoA2), complete cds. (atcc.org)
  • Yu T-W, Chen CW (1993) The unstable melC operon of Streptomyces antibioticus is co-deleted with a Tn4811-homologous locus. (factbites.com)
  • Streptomyces hygroscopicus and Streptomyces viridochromeogenes produce the herbicide bialaphos. (wikipedia.org)
  • How would you define streptomyces-hygroscopicus? (yourdictionary.com)
  • 1. An enzyme extracted and isolated from the microorganism Streptomyces megasporus SD5 or mutant thereof which is isolated by a process which comprises the steps of: a) cultivating the microorganism Streptomyces megasporus SD5 or mutant thereof under aerobic conditions at a temperature in the range of 45° C. to 65° C. in an alkaline aqueous nutrient medium and b) recovering the enzyme. (freepatentsonline.com)
  • 13. An enzyme extracted and isolated from the microorganism Streptomyces megasporous SD5 which is a serine protease of 264 amino acids and has a molecular weight of about 35 kDa as determined by SDS-PAGE. (freepatentsonline.com)
  • GLUCOSE ISOMERASE (Streptomyces violaceoniger) Explanation This enzyme preparation has not previously been reviewed by the Joint FAO/WHO Expert Committee on Food Additives. (inchem.org)
  • Modification of the Streptomyces R61 DD-peptidase by N-bromosuccinimide resulted in a rapid loss of enzyme activity. (portlandpress.com)
  • Large enzyme complexes composed of a number of component enzymes that are found in STREPTOMYCES which biosynthesize MACROLIDES and other polyketides. (bioportfolio.com)
  • Streptomyces offers potential advantages including superior secretion mechanisms, higher yields, a simpler end-product purification process, making Streptomyces an attractive alternative to E. coli and Bacillus subtilis. (wikipedia.org)
  • Thus, Streptomyces is a rich source of the secondary metabolites in which common intermediates in the cell (amino acids, sugars, fatty acids, terpenes, etc.) are condensed into more complex structures by defined biochemical pathways. (pnas.org)
  • Streptomyces mashuensis (originally classified as Streptoverticillium mashuense is typically associated with the production of the antibacterial metabolites, streptomycin and monazomycin. (sciencephoto.com)
  • Fungi trigger Streptomyces exploratory growth in part by altering the composition of the growth medium, and Streptomyces explorer cells can communicate this exploratory behaviour to other physically separated streptomycetes using an airborne volatile organic compound (VOC). (elifesciences.org)
  • The groups of Dr Marcin Szafran and Professor Dagmara Jakimowicz at the University of Wrocław, with Dr Tung Le, Group Leader, and Dr Kim Findlay, Head of the Bioimaging platform at the John Innes Centre has led to a breakthrough in understanding how Streptomyces chromosomes fold which is published in Nature Communications . (jic.ac.uk)
  • It is exciting to see substantial changes in the folding of the chromosomes during the Streptomyces life cycle, from being open to becoming more closed and compacted in preparation for cell division. (jic.ac.uk)
  • Here, we show interactions between Streptomyces and fungi trigger a previously unobserved mode of Streptomyces development. (elifesciences.org)
  • It is because of technical advances in isolation, fermentation, spectroscopy, and genomic studies which led to the efficient recovering of Streptomyces and their new chemical compounds with distinct activities. (hindawi.com)
  • Extreme Streptomyces have been an excellent source of a new class of compounds which include alkaloids, angucycline, macrolide, and peptides. (hindawi.com)
  • However, depressingly in the last decades, the continual rediscovery of similar and known compounds from terrestrial Streptomyces has resulted. (hindawi.com)
  • Streptomyces are remarkable in terms of the morphological and metabolic differentiation phenomena that they present. (factbites.com)