A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens).
The rate dynamics in chemical or physical systems.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.
The resistance that a gaseous or liquid system offers to flow when it is subjected to shear stress. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A colorless liquid made by oxidation of aliphatic hydrocarbons that is used as a solvent and chemical intermediate.
The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Measurement of the intensity and quality of fluorescence.
A worm-like blind tube extension from the CECUM.
Substances that are recognized by the immune system and induce an immune reaction.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Tumors or cancer of the APPENDIX.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
Tumors or cancer of the COLON.
A continuing periodic change in displacement with respect to a fixed reference. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A group of inosine ribonucleotides in which the phosphate residues of each inosine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
The mulberry plant family of the order Urticales, subclass Hamamelidae, class Magnoliopsida. They have milky latex and small, petalless male or female flowers.
The systematic study of the complete DNA sequences (GENOME) of organisms.
A plant genus in the family VITACEAE, order Rhamnales, subclass Rosidae. It is a woody vine cultivated worldwide. It is best known for grapes, the edible fruit and used to make WINE and raisins.
Fermented juice of fresh grapes or of other fruit or plant products used as a beverage.
A plant family of the order Salicales, subclass Dilleniidae, class Magnoliopsida. The leaves are alternate and simple. Staminate (male) flowers consist of from one to many stamens. Pistillate (female) flowers consist of a one-chambered ovary with several to many ovules (potential silky seeds).
A group of FLAVONOIDS derived from FLAVONOLS, which lack the ketone oxygen at the 4-position. They are glycosylated versions of cyanidin, pelargonidin or delphinidin. The conjugated bonds result in blue, red, and purple colors in flowers of plants.
The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.
Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
c-Kit positive cells related to SMOOTH MUSCLE CELLS that are intercalated between the autonomic nerves and the effector smooth muscle cells of the GASTROINTESTINAL TRACT. Different phenotypic classes play roles as pacemakers, mediators of neural inputs, and mechanosensors.
Methods of creating machines and devices.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES.
A species of parasitic EUKARYOTES that attaches itself to the intestinal mucosa and feeds on mucous secretions. The organism is roughly pear-shaped and motility is somewhat erratic, with a slow oscillation about the long axis.
Techniques used in microbiology.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.

A simple hydroponic culture method for the development of a highly viable root system in Arabidopsis thaliana. (1/7660)

In the studies of nutritional absorption and metal toxicity in the root, it is important to grow plants without technical damage. We established a simple hydroponic culture system for Arabidopsis thaliana to obtain a healthy plant having a well-developed root system with many lateral roots. The phytotoxic effects of Cr, Cu, and Al ions were examined by FDA-PI staining using this culture system. The pattern of root inhibition varied with the ion, suggesting the usefulness of this culture system.  (+info)

Inhibition of IL-6 and IL-8 induction from cultured rheumatoid synovial fibroblasts by treatment with aurothioglucose. (2/7660)

Gold compounds have long been used in the treatment of rheumatoid arthritis (RA). However, their actions in RA have not been clarified. In this study, we examined the effect of one of the monovalent gold compounds, aurothioglucose (AuTG), on the IL-1-induced production of IL-6, IL-8 and granulocyte macrophage colony stimulating factor (GM-CSF) from rheumatoid synovial fibroblasts (RSF) isolated from three RA patients. IL-6 and IL-8 induction but not GM-CSF induction was inhibited in most of the RSF after pretreatment with AuTG. Since gene expression of these cytokines is known to be under the control of a common transcription factor, NF-kappaB, the effect of AuTG on the cellular localization of NF-kappaB (p65 subunit) and on NF-kappaB-DNA binding was examined. Although AuTG treatment did not prevent NF-kappaB nuclear translocation, AuTG blocked the DNA-binding activity of NF-kappaB when examined in vitro. Morphologically, both metal-specific cell staining using p-dimethylaminobenzylidene rhodamine and transmission electron microscopic examinations demonstrated the accumulation of metal gold in the cytoplama and some organella (mitochondria and lysosomes) of the AuTG-treated RSF. These results indicate that one of the anti-rheumatic actions of AuTG might be through its inhibitory action on NF-kappaB.  (+info)

Murine cytomegalovirus immediate-early promoter directs astrocyte-specific expression in transgenic mice. (3/7660)

Murine cytomegalovirus (MCMV), which causes acute, latent, and persistent infection of the natural host, is used as an animal model of human cytomegalovirus (HCMV) infection. Transcription of MCMV immediate-early (IE) genes is required for expression of the early and late genes and is dependent on host cell transcription factors. Cell-type-specific expression activity of the MCMV IE promoter was analyzed in transgenic mice generated with the major IE (MIE) enhancer/promoter involving nucleotides -1343 to -6 (1338 bp) connected to the reporter gene lacZ. Distinct expression was observed in the brain, kidneys, stomach, and skeletal muscles. Weak expression was observed in a portion of the parenchymal cells of the salivary glands and pancreas, and expression was hardly detected in the lungs, intestine, or immune and hematopoietic organs such as the thymus, spleen, lymph nodes, and bone marrow. The spectrum of organs positive for expression was narrower than that of the HCMV MIE promoter-lacZ transgenic mice reported previously and showed a greater degree of cell-type specificity. Interestingly, astrocyte-specific expression of the transgene was observed in the brain and primary glial cultures from the transgenic mice by combination of beta-galactosidase (beta-Gal) expression and immunostaining for cell markers. However, the transgene was not expressed in neurons, oligodendroglia, microglia, or endothelial cells. Furthermore, the beta-Gal expression in glial cultures was stimulated significantly by MCMV infection or by addition of calcium ionophore. These observations indicated that expression activity of the MCMV IE promoter is strictly cell-type specific, especially astrocyte-specific in the brain. This specific pattern of activity is similar to that of natural HCMV infection in humans.  (+info)

Tumor extension and cell proliferation in adenocarcinomas of the lung. (4/7660)

To elucidate the mechanism of tumor extension in human pulmonary adenocarcinoma, we immunohistochemically investigated the expression of cell cycle regulator proteins in 54 small adenocarcinomas less than 3 cm in diameter. The Ki-67-labeling index was significantly higher in the periphery of the tumor nodule than in the center. This proliferative potential correlated well with coexpression of cdk2 and cyclin A. p27, one of the cdk inhibitors, was highly expressed in normal bronchial epithelial cells. Peripherally located tumor cells expressed p27 at an intermediate level, but at a higher frequency and level than those in the center. Expression of p21 was also predominant in the periphery. Furthermore, the expression patterns of p21 and p27 were reciprocal. In vitro kinase assays further demonstrated higher cdk2 kinase activity in the periphery. These results suggest that: (i) within an emerging extension made up of peripherally located tumor cells, their high proliferative potential gradually wanes as their relative topographical position becomes more central in the expanding tumor; (ii) peripherally located tumor cells maintain their proliferative potential by higher cyclin A-cdk2 complex activity; and (iii) intermediate expression of p21/p27 in the peripherally located cells promotes higher cyclin A-cdk2 kinase activity, whereas high p21/p27 expression in nonneoplastic cells inhibits kinase activity.  (+info)

Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium. (5/7660)

In epidermis, it has been suggested, intercellular communication through gap junctions is important in coordinating cell behavior. The connexins, may facilitate selective assembly or permeability of gap junctions, influencing the distribution of metabolites between cells. Using immunohistochemistry, we have compared the distribution of connexins 26 and 43 with that of proliferating cells (Ki67 labeling) in normal epidermis, hyperplastic epidermis (tape-stripped epidermis, psoriatic lesions, and viral warts), and vaginal and buccal epithelia. Connexin 43 was abundant in spinous layers of all epidermal specimens and in vaginal and buccal epithelia. Connexin 26 was absent from the interfollicular and interductal epidermis of normal hair-bearing skin, and nonlesional psoriatic epidermis but present at very low levels in plantar epidermis. Connexin 26 was prominent in lesional psoriatic epidermis and viral warts and in vaginal and buccal epithelia. In three independent experiments connexin 26 appeared in a patchy intercellular distribution in the basal epidermis within 24 h of tape stripping, proceeding to more extensive distribution in basal and suprabasal layers by 48 h. The increase in connexin 26 preceded that in cell proliferation. In vaginal epithelium, buccal epithelium, and viral warts connexin 26 was restricted mainly to suprabasal, nonproliferating cells. In psoriatic lesional epidermis connexin 26 was also located mainly in suprabasal, nonproliferating cells. Connexin 26 was present in a patchy distribution in the basal layer of psoriatic lesional epidermis, but double labeling for connexin 26 and Ki67 showed that many connexin 26 positive basal cells were nonproliferative, suggesting that connexin 26 may be related to differentiation rather than to proliferation. These observations would be consistent with a role for connexin 26 containing gap junctions during both early and later stages of keratinocyte differentiation in hyperplastic epidermis and in vaginal and buccal epithelia.  (+info)

Targeted disruption of fibronectin-integrin interactions in human gingival fibroblasts by the RI protease of Porphyromonas gingivalis W50. (6/7660)

Cell surface integrins mediate interactions between cells and their extracellular matrix and are frequently exploited by a range of bacterial pathogens to facilitate adherence and/or invasion. In this study we examined the effects of Porphyromonas gingivalis proteases on human gingival fibroblast (HGF) integrins and their fibronectin matrix. Culture supernatant from the virulent strain W50 caused considerably greater loss of the beta1 integrin subunit from HGF in vitro than did that of the beige-pigmented strain W50/BE1. Prior treatment of the W50 culture supernatant with the protease inhibitor Nalpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) blocked its effects on cultured cells, indicating that this process is proteolytically mediated. Purified arginine-specific proteases from P. gingivalis W50 were able to mimic the effects of the whole-culture supernatant on loss of beta1 integrin expression. However purified RI, an alpha/beta heterodimer in which the catalytic chain is associated with an adhesin chain, was 12 times more active than RIA, the catalytic monomer, in causing loss of the alpha5beta1 integrin (fibronectin receptor) from HGF. No effect was observed on the alphaVbeta3 integrin (vitronectin receptor). The sites of action of RI and RIA were investigated in cells exposed to proteases pretreated with TLCK to inactivate the catalytic component. Use of both monoclonal antibody 1A1, which recognizes only the adhesin chain of RI, and a rabbit antibody against P. gingivalis whole cells indicated localization of RI on the fibroblasts in a clear, linear pattern typical of that seen with fibronectin and alpha5beta1 integrin. Exact colocalization of RI with fibronectin and its alpha5beta1 receptor was confirmed by double labeling and multiple-exposure photomicroscopy. In contrast, RIA bound to fibroblasts in a weak, patchy manner, showing only fine linear or granular staining. It is concluded that the adhesin component of RI targets the P. gingivalis arginine-protease to sites of fibronectin deposition on HGF, contributing to the rapid loss of both fibronectin and its main alpha5beta1 integrin receptor. Given the importance of integrin-ligand interactions in fibroblast function, their targeted disruption by RI may represent a novel mechanism of damage in periodontal disease.  (+info)

Adaptive immune response to Shigella flexneri 2a cydC in immunocompetent mice and mice lacking immunoglobulin A. (7/7660)

Shigella flexneri cydC, which is deficient in cytochrome bd, was rapidly cleared from the lungs of intranasally inoculated mice and was Sereny negative, yet it induced 93% protection against challenge with wild-type S. flexneri. Mice that lack immunoglobulin A (IgA) were fully protected, suggesting that IgA may not be required for adaptive immunity in this model system.  (+info)

CNS wound healing is severely depressed in metallothionein I- and II-deficient mice. (8/7660)

To characterize the physiological role of metallothioneins I and II (MT-I+II) in the brain, we have examined the chronological effects of a freeze injury to the cortex in normal and MT-I+II null mice. In normal mice, microglia/macrophage activation and astrocytosis were observed in the areas surrounding the lesion site, peaking at approximately 1 and 3 d postlesion (dpl), respectively. At 20 dpl, the parenchyma had regenerated. Both brain macrophages and astrocytes surrounding the lesion increased the MT-I+II immunoreactivity, peaking at approximately 3 dpl, and at 20 dpl it was similar to that of unlesioned mice. In situ hybridization analysis indicates that MT-I+II immunoreactivity reflects changes in the messenger levels. In MT-I+II null mice, microglia/macrophages infiltrated the lesion heavily, and at 20 dpl they were still present. Reactive astrocytosis was delayed and persisted at 20 dpl. In contrast to normal mice, at 20 dpl no wound healing had occurred. The rate of apoptosis, as determined by using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling, was drastically increased in neurons of ipsilateral cortex of the MT-I+II null mice. Our results demonstrate that MT-I+II are essential for a normal wound repair in the CNS, and that their deficiency impairs neuronal survival.  (+info)

A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties. In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. In a more strict sense, the term vital staining has a meaning contrasting with supravital staining. While in supravital staining the living cells take up the stain, in vital staining - the most accepted but apparently paradoxical meaning of this term, the living cells exclude the stain i.e. stain negatively and only the dead cells stain positively and thus viability can be assessed by counting the percentage of total ...
Sent: Thursday, January 09, 2003 2:43 PM Subject: Fe stain on BM > I would like to know if other labs are doing iron stains on the clot and core > of bone marrow biopsies. > > We normally do a Prussian blue iron stain on the clot, core, and 1 smear. > Today one our pathologist said they read that this stain actually does not > stain iron in bone cores and clots. Our control is liver and processed in the > same manner as the clot, and the only difference with the core is the 10-15 > minute decal we do just prior to cutting (after processing and embedding). > Are we in fact getting false negatives? > Should I be doing a different stain for iron in bone core biopsies? > Any help would be greatly appreciated. > P.S. I have seen positive staining in some cores here, but the pathologist > claimed that was erroneous staining! > > Cindy DuBois, HT > Delta Pathology Assoc. > Stockton, CA > (209)477-4432 ...
The microvessel density (MVD) was assessed in lymph nodes infiltrated by diffuse large B-cell lymphomas, mantle cell lymphomas, chronic lymphatic leukemia and follicular lymphomas, and in lymphadenitis. Serial sections of formalin-fixed and paraffin-embedded tissue were stained with antibodies against CD31, CD34 or Factor VIII. Using light microscopy and computerised image analysis, the number and size of individual immunostained vessel profiles within a preselected area size range corresponding to capillaries, postcapillary venules, small collecting venules and small arterioles were determined. A significantly larger number of vessels were registered following staining with anti-CD34 than with anti-CD31 or anti-Factor VIII. Moreover, among the smallest capillary-sized vessel profiles in all lesion types, there was a selective relative loss of stainability of anti-CD31 and anti-Factor VIII, resulting in a substantial total loss of visualised capillary-sized vessels compared with anti-CD34. In ...
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Clean stains from a marble countertop by applying a specially prepared poultice to wick the stain and then applying a new sealant. The poultice removes the stain from the stone pores while the...
01/31/05 20:41 PM >>> I need some help!! We have been experiencing some yeast and bacteria showing up on some of our special stains. It started about 2 weeks ago and seems to be sporadic (one day...no problems, the next day, several stains are affected). The yeast and bacteria (rods) are clumped together all over the slide, on the tissue sections, around the sections and on the slide in areas where there is no tissue. The stains we have seen the contamination are the Gram, PAS/D, PAS and GMS. All of these stains are automated except the Gram, so I do not think the special stainer is the problem. Here is what I have eliminated as problem areas: -Processor solutions and paraffin (we cut and stained a block from another institute and had contamination) -Waterbaths, slide drying boards, water containers (used to fill waterbathes), stainer containers, ice pans (all of the mentioned have been thoroughly bleached) -Tap water and DI water (all water supplies have been tested by sending a sample to ...
Ridana @ # 21 - Okay, I zoomed in on the fallen soldier next to the ammo(?) box, and have to agree that a little blood does show on his face. Not so sure about the face-planter - the stains on his back seem much the same as the stains on his elbow…. Youre also right about the flying hat: I had thought that the head of the man behind the one with his gun still on his shoulder was that mans head. And I took what you see as smudges as light/shadow effects - wed have to interrogate the artist very closely to make sure.. I see the uniforms as well-laundered mostly because I compare them to, e.g., Matthew Brady photographs, and written descriptions of the poorly-equipped troops on both sides of the Unpleasantness Between the States. It surely also influenced me to look up Troianis website and see that whitewashing the ugliness of war is his consistent pattern, regardless of his claim (DON TROIANI is a traditional academic realist painter well known for his extremely accurate historical and ...
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The erection of a sign out the front of a Richmond business has caused a big stink among the local council who have ordered it must come down.
Vital staining refers to staining methods carried out on living animals or living cells. Intravital staining means staining is performed on a living object. Supravital staining is a method which is used on cells with limited vitality or tissue which has been removed from an animal.. The stain is usually applied by injection (intravenous, intraperitoneal, subcutaneous, or subdural) for live staining or by feeding (oral or enteral with a pharangeal tube). Supravital staining is carried out on organs or cells which have been kept alive and immersed in or perfused with a staining solution.. The available vital stains consist of:. ...
A breif guide to immunohistochemistry protocol,including tissue collection, Fixation,Embedding, Sectioning, Mounting, Antigen retrieval, antibody reaction, Staining, Counterstaining and sealing.
OG-6 is a cytoplasmic counterstain solution used in sequence with EA (EA 50, EA 65 or EA36) in the Papanicolaou staining method for clinical cytology. Gills modified OG-6 is stable in solution and gives predictable high quality staining results not previously possible with other formulations.. Learn More ...
OG-6 is a cytoplasmic counterstain solution used in sequence with EA (EA 50, EA 65 or EA36) in the Papanicolaou staining method for clinical cytology. Gills modified OG-6 is stable in solution and gives predictable high quality staining results not previously possible with other formulations.. 了解更多 ...
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Summary Many companies have articulated a set of values. Their role ranges from covering stains on company walls to actually defining how the company does things. In most companies, one value reigns supreme. What that value is depends mostly on how the company is led and the authenticity of that particular value. Sometimes it is a value that […]. ...
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In article ,01HAMMGE7PIQ005655 at GW.AGR.CA, BISSETTJ at NCCCOT2.AGR.CA (John Bissett) writes: , An industry client has an urgent need of a permeant vital , stain for Saccharomyces cerevisiae and other yeasts. Their , automated imaging system cannot reliably distinguish yeasts , on grid membrane filters from dust and other , autofluorescing contaminants. The stain should not be , toxic to yeasts. A fluorescent brightener should have an , emission range other than 415-505 nm, the range of , autofluorescence in this application. , , Has anyone knowledge of vital stains/optical brighteners , for this application - or another solution? , , Please address replies to: BISSETTJ at NCCCOT2.AGR.CA , , Thanks , , John Bissett An excellent source for a large variety of dyes is a company called Molecular Probes (Eugene, Oregon). A group of dyes you should consider are the potentiometric cyanine dyes, some of which report the energization of the mitochondrial membrane and which are easily measured with ...
TY - JOUR. T1 - An approach to immunomicroscopy of renal disease with immunoperoxidase and periodic acid-Schiff counterstain (IMPAS stain). AU - Tubbs, R. R.. AU - Gephardt, G.. AU - Valenzuela, R.. AU - Deodhar, S.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - A series of 127 renal specimens reflecting a wide spectrum of renal pathology was evaluated with a direct immunoperoxidase technic in which frozen tissue was first reacted with anti-Ig(G,A,M) and anti-C3-peroxidase conjugates and subsequently counterstained with periodic acid-Schiff-hematoxylin (IMPAS). This technic permitted simultaneous evaluation of light-microscopic changes and yielded a permanent immunomicroscopic record. Frequently, a distinction could be made between epimembranous, intramembranous and subendothelial deposits, which was not possible using immunofluorescence. The findings were correlated with histologic, immunofluorescent and electronmicroscopic results. General patterns of immune complex and autoantibody deposition were ...
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Definition of Giemsa stain in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Giemsa stain? Meaning of Giemsa stain as a legal term. What does Giemsa stain mean in law?
After the swapped forearms for Frosta, Mattell has another great design choice: the black stain on Netossas net. On the left the Fwoosh review and on the right the Pixel Dan review: http://img803.imageshack.us/img803/9576/netossproblem.jpg Were so lucky that they improved the quality as they promised during the subscription campaign! Another thats the only Netossa produced so take it or sell it?
Gram method, Gram stain, an empirical staining procedure in which microorganisms are stained with crystal violet, treated with 1:15 dilution of strong iodine solution, decolorized with ethanol or ethanol-acetone, and counterstained with a contrasting dye, usually safranin O. Those microorganisms that retain the crystal violet stain are said to be gram-positive, and those that lose the crystal violet stain by decolorization but stain with the counterstain are said to be gram-negative. Gram-positive organisms that lose the stain easily, so that samples appear to be a mixture of gram-positive and gram-negative organisms, are said to be gram-variable. ...
Theory and Practice of Histological Techniques, 5th. Edition by J.D. Bancroft and M. Gamble http://i.imgur.com/odpv7AD.jpg Page: 768 Publisher: -- Edition: 5th., 2001 Language: English ISBN-10: 0443064350
ISBN: 9789389188479 Pages: 296 The latest edition of Manual of Histological Techniques is a compilation of the basicsand latest advances in this rapidly evolvi
Safranin is used as a dye for textiles and as a stain for various scientific procedures. Scientific procedures that involve safranin include Gram counterstaining, endospore counterstaining, granule...
Eosine Yellowish-(YS): A versatile red dye used in cosmetics, pharmaceuticals, textiles, etc., and as tissue stain, vital stain, and counterstain with HEMATOXYLIN. It is also used in special culture media.
Since heterotrophic prokaryotes play an important biogeochemical role in aquatic ecosystems and have a high capacity to survive in extreme environments, easy-to-perform protocols that probe their physiological states and the effects of environmental variables on those states are highly desired. Some methodologies combine a general nucleic acid stain with a membrane integrity probe. We calibrated one of these, the nucleic acid double-staining (NADS) protocol (G. Gre ́gori, S. Citterio, A. Ghiani, M. Labra, S. Sgorbati, S. Brown, and M. Denis, Appl. Environ. Microbiol. 67:4662-4670, 2001), determining the optimal stain concentrations in seawater and the response to conditions that generate prokaryote death (such as heat) and to conditions that are known to produce death in plankton, such as nutrient limitation or flagellate grazing. The protocol was validated by comparison to two methods used to detect viability: active respiration by 5-cyano 2,3-ditolyl tetrazolium chloride (CTC) and ...
The office chair is often abused. It can easily acquire and accumulate dirt, dust, coffee stains and even sweat stains. Keep in mind that work chairs today do not come cheap. If you dont want to spend money on a new work chair, you have to keep your current one clean and well-maintained.. If there are stains, clean them immediately. Here are few tips on how to do that:. Tip 1: Sweat stains can be removed through vinegar. Push your chair into a cleaning area. Mix one cup of distilled white vinegar and one cup of lukewarm water. To evenly apply the solution, place it in a spray bottle. Spray it all over the stained areas of the seat cloth. Let the acids in the vinegar work in removing sweat stains. It usually takes an hour or so to finish this task.. After an hour, remove the contents of the spray bottle and fill it up with water. Spray water on the chair but do not soak the foams completely. Just spray it enough to wash off some of the solution residues. Leave your chair in a nice and warm room ...
Silver nitrate is a chemical substance that is used to develop photographs and in some medical procedures. If the substance comes into contact with the skin, it leaves a residue...
Im new to this board, and I think I originally posted this question in the wrong place.. I bought a 50 year-old house 4 years ago. I immediately took up the wall-to-wall carpet to find beautiful hardwood floors in pristine condition. I had the floors professionally sanded and polyurethaned. Now 4 years later I have 5 or 6 dark urine stains from my new dog. How can I remove these stains. I dont want to just cover them with carpet or scatter rugs. Can anybody help me ...
*筱瑤*娛樂地帶 organic gemstones including pearls. after wearing gold stains and dust often contaminated by and lose luster. About 6 hours in police Dunshou, you can ... - Discuz! Board
Apply the mixture to the stained area using a wood or plastic spatula and allow to dry. Be sure to spread the poultice well beyond the stained area. The liquid portion of the paste will migrate into the concrete where it will dissolve some of the staining material. Then the liquid will gradually move back beyond the concrete surface and into the poultice, where it will evaporate, leaving the dissolved staining material in the poultice ...
Courtney Bisbee in happier times, with her daughter Taylor Lee. The case of convicted student-boffer Jennifer Mally makes my blood boil. Not because I...
Light microscopy makes use of primarily two detection systems for immunohistochemistry - fluorescence and enzyme labeling, while electron microsco ...
a-f) Brain sections from Psen1tm1.1Tcs/Psen1tm1.1Tcs Tg(APPSWE)2576Kha/0 mice (a,b,c,e) and Tg(APPSWE)2576Kha/0 mice (d,f). Psen1tm1.1Tcs/Psen1tm1.1Tcs Tg(APPSWE)2576Kha/0 mice (3 (a), 6 (b) and 9 months (c,d) old) and 9-month-old single Tg(APPSWE)2576Kha/0 mice (d,f) were immunostained with the 4G8 antibody to amyloid beta (a-d) and antibody to GFAP (green) with 4G8 counterstaining (red) (e,f ...
The culture of A-10 cells illustrated in this section was labeled with MitoTracker Red CMXRos before fixing, and the cells were subsequently stained with Alexa Fluor 488 conjugated to phalloidin, followed by Hoechst 33258, a popular DNA-binding counterstain.
Use as a counterstain during Ziehl-Neelsen and Kinyoun staining. Pro-Lab Diagnostics™ Malachite Green Stain is a ready-to-use stain to differentiate non-acid-fast organisms from mycobacterium. It is manufactured to the highest standards, according to all regulatory requirements, and CE labelled. 500 ML Acid fast Stain for Mycobacteria Malachite ...
Viklicky, V and Bagarova, I, Mast cells in the spleen of mice of the strain a/ph. The effect of fixation on their stainability. (1969). Subject Strain Bibliography 1969. 1870 ...
Using a counting chamber does not necessarily yield an accurate count of the number of live cells because it is not always possible to distinguish between live cells, dead cells, and debris of the same size under the microscope. However, newly developed fluorescence staining techniques make it possible to distinguish viable and dead bacteria. These viability stains (or live stains) bind to nucleic acids, but the primary and secondary stains differ in their ability to cross the cytoplasmic membrane. The primary stain, which fluoresces green, can penetrate intact cytoplasmic membranes, staining both live and dead cells. The secondary stain, which fluoresces red, can stain a cell only if the cytoplasmic membrane is considerably damaged. Thus, live cells fluoresce green because they only absorb the green stain, whereas dead cells appear red because the red stain displaces the green stain on their nucleic acids (Figure 8).. Another technique uses an electronic cell counting device (Coulter counter) ...
The Core Service provides services to FLI researchers for standard histological techniques, including processing of tissues, sectioning and preparation of samples for the EM. EM service for external users is available upon arrangement. Some standard histological stainings are also available, carried out either by the CS staff or by users themselves with guidance provided by the CS staff where appropriate. The major services include paraffin sections, cryo-sections and histological stainings (H&E, Masson-Trichrome, NADH, Sirius Red, COX, Sudan-3). The CS maintains the following equipment:. ...
Opal™ is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
Bakteri gram-negatif adalah bakteri yang tidak mempertahankan zat warna metil ungu pada metode pewarnaan Gram. Bakteri gram-positif akan mempertahankan warna ungu gelap setelah dicuci dengan alkohol, sementara bakteri gram-negatif tidak. Pada uji pewarnaan Gram, suatu pewarna penimbal (counterstain) ditambahkan setelah metil ungu, yang membuat semua bakteri gram-negatif menjadi berwarna merah atau merah muda. Pengujian ini berguna untuk mengklasifikasikan kedua tipe bakteri ini berdasarkan perbedaan struktur dinding sel mereka ...
Opal™ is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
Stains on baby clothing can be really hard to remove without special treatment. This is a guide about stain remover for tough baby stains.
2. More leds, can clearly find the dried urine of pets, the food stains on the carpets, curtains, furniture, and the floor(difficult to find with naked eyes), can also for detecting the cosmetics, masks, baby supplies, money, certificates and so forth ...
Mold Remover Gel is the ideal solution to tough mold stains. The thick gel clings to and penetrates stains on tile, grout, showers, and tubs without harsh fumes or overspray. FAST EFFECT You can effortlessly get rid of those black and brown stains particularly on those sealed joints around your bath or shower and make
Mold Remover Gel is the ideal solution to tough mold stains. The thick gel clings to and penetrates stains on tile, grout, showers, and tubs without harsh fumes or overspray. FAST EFFECT You can effortlessly get rid of those black and brown stains particularly on those sealed joints around your bath or shower and make
Organic cleaners typically do not affect the concrete color or harm the surface because the enzymes only go after organic material, and concrete is as inorganic as you can get. That is why stain removal for concrete is really tough and needs special treatment. Does the sealer appear to be undamaged, and therefore this is probably just a surface stain on the sealer itself? I have tried scrubbing with a brush attached to a power-scrub setting on my hose. This liquid gets into the pores of the concrete, and as it expands, it is forced deeper into the concrete. As far as the sealer beading up, it sounds like some remaining glue residue is causing fish eyeing. references REMR: Ways to Clean Concrete Show Comments you may like. Different people have different recipes and techniques for applying poultices, but they all have the same basic characteristics. Rinse the area well with water. Add a few squirts of dish soap and use your hands to mix up the soap and water. By the next time it rains, the ...
In an attempt to knock out a mild stain on the floor of my deep end, I think I have caused larger issues. It was recomended to me to use Sparkle Conquest to knock out the stain. Pool has always been crystal clear with no signs of algae. All I ever do is adjust my SWG higher when we in the height of the summer and add Muriatic acid to keep my pH down. I never use any other products till now. Heres a timeline of the steps Ive have taken and where I am at now: Friday night I turned off
Have you ever tried to get a stain out of carpet? Not an easy job! There is a small stain on my carpet in our family room. The first time I noticed it I attacked it immediately. I got out all the ammo!! Those who know me know that Im a fanatic about clean carpets.…
Of course the section that interests Mommy most is the children and babies section. Some things are cheap, some are not - you have to choose wisely. Mommy also noticed that some really cheap items (for example Kiki Lala infant clothes selling for RM2) are no longer in good condition in that they have stains on them. Also, many items were not well price-tagged. Mommy saw many tags merely written as PW 03 with no mention of the price.....now what is that supposed to mean? As for payment, only cash term was accepted. But theres no queue for payment although there were also two cashier counters because not many people were buying anyway! Here are some snapshots of the sale: ...
Ex-library edition with the usual stamps and markings on exterior cover and endpapers. Two volumes: a 10 1/8w x 14 1/8h thirty-two page book of text in Very Good condition by George Dock, and a 14 1/4w x 17h book of 25 plates (not 30 as the title indicates) bound into a hardback blue buckram cover by the library, in Good condition, suitable for framing. Some damp stains on the blank backs of the plates that do not affect the prints except as noted... View more info. ...
This is a high energy environment combining every imaginable aspect of fitness. We strategically blend a mix of mobility, strength, power, and endurance to form a smart and challenging program for everyone. As a group, we thrive off one anothers support and use this atmosphere to conjure your inner athlete.. Were unique in our approach by using science, technology, and proven strength and conditioning principles to create an intelligent and rewarding program. Our progressive principles and precision coaching will set you on a guaranteed path for success. By offering more than a gimmicky sweat stain on the floor, we push you the right way and celebrate the result.. As a member of Chaos, you will be exposed to the following:. ...
ARCAMOSS contains powerful fungicides. It is sprayed or brushed onto the moss or mold, and leaves no stains on the materials. ARCAMOSS sanitizes all b...
Besides the regular maintenance, there might be stains on and to do this, you will need to use either a spoon or a dull knife. Fortunately, cleaning t...
Rain‑X® Bug & Tar Pre-Wash Gel visibly fights to remove tough stains on your vehicles surface. The powerful gel clings to your cars surface to effectively dissolve bugs, tar and bird droppings.
Read all 51 questions with answers, advice and tips about Meijer Natural & Homemade Cleaners from moms communities. Some of the advice from Moms is: A Good Disinfectant for Granite, Looking for a Baby-friendly Carpet Stain Remover, Advice on HomeMade Gifts for Family and Friends
Even if you dont notice them until the morning after, beer stains are fairly easy to remove. Find out how with this stain removal advice from the Good Housekeeping Institute.
Grease stains are tricky to get out of your carpet, so it is very important to identify what type of material you will be working with. Patience is crucial and make sure to follow directions well. Here are some methods you can try when removing grease stains. Fist, try and…. ...
Pink stains are commonly found in Minnesota. They show up in toilets, sinks, and tubs. Learn what causes the pink stains, and how to treat them.
General Finishes wood stains are made from the highest quality oils, resins, and pigments to ensure your finishing project will be an easy and ...
Goudey wiping stains are renowned for their depth, vibrancy and richness which have made them the industry benchmark for decades. Goudey offers professionals a broad palate of 36 stock colours that are relevant in both historical and current trends
What are the Romanowsky Stains? Types of Romanowsky Stains- Giemsa stain, Wright and Wright-Giemsa stain, May-Grunwald stain, Leishman stain.
Middle: Nissl-stained motor cortex of a human adult. Right: Golgi-stained cortex of a ​1 1⁄2 month old infant. The Nissl stain ... Cell body layers are labeled on the left, and fiber layers are labeled on the right. ... Staining cross-sections of the cortex to reveal the position of neuronal cell bodies and the intracortical axon tracts allowed ... Stained brain slice images which include the "cerebral cortex" at the BrainMaps project ...
The central and rightmost cell are in interphase, so the entire nuclei are labeled. The cell on the left is going through ... Human cancer cells with nuclei (specifically the DNA) stained blue. ... Swammerdam led to new interest in entomology and helped to develop the basic techniques of microscopic dissection and staining. ...
Stained glass with label moulds over. Good belfry above western parapet gable. Federation Gothic style hall c. 1900s, ... The stained glass windows are some of the earliest to be made in the colony, the two in the first bay of the south wall of the ...
These proteins can be stained with fluorescent dye labeled antibodies and detected using flow cytometry. The limit of detection ...
Analogous methods to western blotting can be used to directly stain specific proteins in live cells or tissue sections.[24][25] ... The membrane is then exposed to a labeled DNA probe that has a complement base sequence to the sequence on the DNA of interest. ... In this process RNA is separated based on size and is then transferred to a membrane that is then probed with a labeled ... The target DNA is then washed and the labeled probes that didn't hybridize are removed. The target DNA is then analyzed for the ...
Crest stated that because of the low incidence of staining, there was no need to label Pro-Health mouthwash as a potential ... Tooth staining[edit]. Crest Pro-Health mouthwash contains Cetylpyridinium chloride which is known to cause tooth staining in ... antimicrobial rinses may cause surface staining to teeth," but consumers have complained the warning label is buried in the ... the mouthwash now contains a label warning consumers of its potential to stain teeth.[20] ...
Nuclei are stained blue, mitochondria are stained red, and microfilaments are stained green. ... The central and rightmost cell are in interphase, so their DNA is diffuse and the entire nuclei are labelled. The cell on the ... Capsules are not marked by normal staining protocols and can be detected by India ink or methyl blue; which allows for higher ... Staining of a Caenorhabditis elegans which highlights the nuclei of its cells. ...
... or label; soil and stains; dogeared pages; underlining, highlighting, and marginalia; water damage; torn hinges, endpapers and ... Previous owners of books often signed their copies or labelled them with bookplates, and it is often not difficult to identify ...
... an INXS covers single on Australian label Stained Circles; a Sparks covers single on In The Red, which includes "Sherlock ... A companion remix album was released by Scion's Scion A/V label. In September 2013, Ooey Gooey Chewy Ka-blooey!, the long- ... Los Angeles-based independent label In The Red Records persuaded the Dirtbombs to record an LP. Collins decided that if the ... Five singles were issued in 2008: a cover of a Suicide song on the Irish label Infirmary Phonographic; ...
After staining with heavy metal labels, Sjöstrand et al. noted two thin dark bands separated by a light region, which they ... They fused two cells labeled with different membrane-bound fluorescent tags and watched as the two dye populations mixed. The ...
A label mould finishes the top of the openings. The east wall of the church has three tall arched openings. All the windows to ... The Cathedral's present day interior contains church furniture, furnishings and stained glass windows as well as a number of ... There are significant memorials located within the Cathedral's interior, including the stained glass windows memorial tablets. ... the church have stained glass. At the eaves and near the top of the parapet walls, the stonework is finished with a dentilated ...
... a next-generation holographic microscope for 3D live cell imaging without the need for staining or labeling. The HT-1 is the ... "Holographic Microscope Allows Stain-Free 3D Imaging of Live Cells". Asian Scientist Magazine. 4 April 2016. Retrieved 1 April ... "TomoCube holotomography offers label-free imaging of cells". optics.org. Retrieved 1 April 2017. " ...
In 2010, he created Stained Music, his own record label. Today, Stainer is heralded as one of the pioneers of the house music ... The first track they produced, "I Don't Want It to End" was picked up by the record label EMI / Universal Music Group and ... In 2012, Stainer released the single "Can't Take That Away" on the Hed Kandi label featuring the vocals of Roland Clark. As of ... In 2011, "Piracy Part 2" released on Stained Music received support from the following major artists: Groove Armada, Tocadisco ...
Biological staining[edit]. OsO4 is a widely used staining agent used in transmission electron microscopy (TEM) to provide ... Label with poison warning. OsO4 is highly poisonous, even at low exposure levels, and must be handled with appropriate ... Polymer staining[edit]. It is also used to stain copolymers preferentially, the best known example being block copolymers where ... oxide is also used as a stain for lipids in optical microscopy.[21] OsO4 also stains the human cornea (see safety ...
The central and rightmost cell are in interphase, so the entire nuclei are labeled. The cell on the left is going through ... Human cancer cells with nuclei (specifically the DNA) stained blue. ...
An image of a newt lung cell stained with fluorescent dyes during metaphase. The mitotic spindle can be seen, stained green, ... A cross section of a nuclear pore on the surface of the nuclear envelope (1). Other diagram labels show (2) the outer ring, (3 ... HeLa cells stained for nuclear DNA with the blue fluorescent Hoechst dye. The central and rightmost cell are in interphase, ... The nucleolus is the largest of the discrete densely stained, membraneless structures known as nuclear bodies found in the ...
... is the debut album by Japanese metal band Blood Stain Child. It was released in 2002, by the Captain ... Rock label. "Silence of Northern Hell" - 3:41 "Crimson Symphony" - 4:45 "Under the Sin of Grief" - 5:42 "Legend of Dark" - 4:03 ... Blood Stain Child , Releases , AllMusic". AllMusic. Retrieved January 10, 2014. CS1 maint: discouraged parameter (link) v t e. ...
Fragmentation and labeling of the PCR product Hybridization and staining on the AmpliChip DNA microarray. Scanning the chip. ...
See also: Fluoro-Jade stain. In cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track ... Fluorescein-labelled probes can be imaged using FISH, or targeted by antibodies using immunohistochemistry. The latter is a ... The use of fluorescein amidite, shown below right, allows one to synthesize labeled oligonucleotides for the same purpose. Yet ... Brush, C. K. "Fluorescein Labelled Phosphoramidites". U.S. Patent 5,583,236. Priority date July 19, 1991. ...
He also respects Stain's wishes as he protected Izuku from Kenji, because Stain had labeled him as someone worthy of being ... Chizome Akaguro (赤黒 血染, Akaguro Chizome) / Stain, The Hero Killer (ヒーロー殺し ステイン, Hīrō Goroshi Sutein). Voiced by: Go Inoue[25] ( ... "My Hero Academia Season 2 Casts Go Inoue as Stain". Anime News Network. June 1, 2017. Retrieved June 1, 2017.. ... He doesn't like Stain and believes they'll never get along, despite the anti-villain's indirect contribution to the League in ...
One third of these lesions is present at birth as a telangiectatic stain or ecchymotic area. During the first four weeks of ... Historically, vascular anomalies have been labeled with descriptive terms, according to the food they resembled (port wine, ... Capillary malformation (also known as port-wine stains): Capillary malformations are flat, reddish lesions that typically ... Superficial vascular anomalies appear as red-coloured stains and are associated with vascular anomalies affecting the dermis. ...
Mason did not record another album until 2003's Stained Glass Window, on the independent Twinbeat label. Mila's mother, Diane, ... she signed to the independent Twinbeat Records label, recording the album Stained Glass Window. This album also included her ...
Fear Factory vocalist Burton C. Bell provides vocals on the track "Stain". Spineshank joined Fear Factory and Kilgore on a ... As a result of these shows, Spineshank gathered interest from several record labels. Eventually, the band signed with ...
It is insoluble in organic compounds, and can be labeled by silver staining. It is called neuromelanin because of its function ...
Digital holography has been used in different contexts to study cell morphology using neither staining nor labeling. This can ... Label-free high content analysis. Fluorescent high content analysis/screening has several drawbacks. Label-free alternatives ... Label-free cell cycle analysis. The phase shift induced by cells has been shown to be correlated to the cell dry mass. The cell ... Label-free viability analysis of adherent cell cultures. Digital holography has been used to study the apoptotic process in ...
Staining and labeling techniques are often used in microscopy to help identify specific structures in a cell. Staining usually ... In the case of dendritic spikes, staining and labeling are used to identify and quantify the presence of certain voltage-gated ... Labeling involves the use of fluorescence to identify specific molecules. Fluorophores, fluorescent molecules, may be directly ...
"Application of the DNA-specific stain methyl green in the fluorescent labeling of embryos". Journal of Visualized Experiments ( ... Fluorescent DNA staining is routinely used in cancer prognosis. Methyl green also emerges as an alternative stain for DNA in ... It has been used for staining cell nuclei either as a part of the classical Unna-Pappenheim stain, or as a nuclear counterstain ... Methyl green (CI 42585) is a cationic or positive charged stain, related to Ethyl Green, that has been used for staining DNA ...
The cerebral organoid neurons can, however, form axons as shown by GFP staining. GFP labeled axons have been shown to have ... Many regions that have forebrain marker FOXG1, labeling them as regions with cerebral cortical morphology, were also positive ...
... "their voices fuse like sunlight beaming through a stained-glass window." James Hunter of Rolling Stone said that it is " ... Cary Darling from Orange County Register labeled it as a "soaring, wide-screen ballad." Jan DeKnock from Orlando Sentinel ...
He also designed wine labels for the "Graphische Anstalt Wilhelm Gerstung" in Offenbach. Throll died in Munich at the age of 81 ... In 1919, he painted the Offenbach Synagogue and also executed the stained glass windows and the Torah shrine. ...
USA Home > Product Directory > Biochemicals and Reagents > Fluorescent Probes, Labels, Particles and Stains > Fluorescent ...
USA Home > Product Directory > Biochemicals and Reagents > Fluorescent Probes, Labels, Particles and Stains > Fluorescent ...
4-(4-(Dimethylamino)-styryl)-N-methylpyridinium iodide; stains mitochondria in live cells ...
PromoCell provides a variety of cell staining reagents as well as fluorophores for easy labeling of diverse biomolecules ... Cell Staining & Fluorescent Labeling. The availability of new fluorophores has dramatically changed the possibilities for the ... PromoCell provides a variety of reagents for staining different cell organelles. and structures as well as for determining ... We also offer a wide range of high quality fluorophores for easy labeling of diverse biomolecules including proteins, ...
Cell Surface Stain. Covalently labeling of the surface of live cells, withstanding fixation and permeabilization for subsequent ... Biotin PCR Labeling Kit. Kit for biotin-labeling of DNA by PCR. Available in 175 reactions size. ... Biotin-labeled UTP. Biotin-16-UTP conjugate (10 mM) for RNA labeling by in vitro transcription. ... Blue-fluorescent Cytoplasmic Membrane Staining Kit. Convenient and sensitive cell staining with low cytotoxicity. ...
Basic Protocol 1: Viability Staining of Cell Suspension Using One Dye. *Basic Protocol 2: Viability Staining of Cell Suspension ... S869 for the FITC‐labeled conjugate or S11223 for Alexa Fluor 44-labeled conjugate) ... Fluorochrome‐labeled secondary (anti‐IgG) Ab (directed against primary Ab species; can be purchased from Sigma, Pierce, R&D ... Fluorochrome‐labeled primary (antigen specific) antibody (can be purchased from Pierce, R&D Systems, or Accurate Chemicals) ...
Virtual Cell Staining Tool Read More + Image was created using the new online cell staining simulation tool with Molecular ... In this schematic, cells are labeled orange with CellTracker™ Orange dye, and nuclei are stained blue with Hoechst 33342.. ... In this schematic, cells are labeled orange with CellTracker™ Orange dye, and nuclei are stained blue with Hoechst 33342.. ... Labeling Chemistry Selection Tool-NEW! Quickly find the best reactive dye or label for your experiment. Whether you are ...
... virtual staining, that improves the Raman spectral histo Optical Diagnosis ... The great capability of the label-free classification of tissue via vibrational spectroscopy, like Raman or infrared imaging, ... Virtual staining of colon cancer tissue by label-free Raman micro-spectroscopy D. Petersen, L. Mavarani, D. Niedieker, E. ... Virtual staining of colon cancer tissue by label-free Raman micro-spectroscopy ...
Buy Stains, Dyes and Fluorescent Probes, for use in immunoassays including Flow Cytometry, Immunofluorescense and ... Stains, Dyes, Probes & Labels. View our specific categories below to easily locate the right Stain, Dye, Probe or Label for ... High quality Stains, Dyes, Fluorescent Probes and Labels are available for use in various immunoassays including Flow Cytometry ...
Mix-n-Stain™ CF® Dye Antibody Labeling Kits From: $103 Sizes: 5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug ... Antibody & Protein Labeling Kits. Protein Labeling Kits , Small Ligand Labeling Mix-n-Stain™ Antibody Labeling Kits ... Mix-n-Stain™ CF® Dye Antibody Labeling Kits Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling ... Mix-n-Stain™ Antibody Labeling Kits Mix-n-Stain™ Antibody Labeling Kits use revolutionary technology that dramatically ...
Evaluate fluorescently-labeled tissues. Slide scanning has traditionally been performed with colorimetric stains, but many ... Automated slide scanning of histologically stained and fluorescently labelled tissues *Locate key regions of interest by ... Youll also have the flexibility to image and analyze both fluorescently-labeled and histologically-stained tissues. Ready when ... Figure 2. (A) Preview scan of H&E stained lung tissues using transmitted light and RGB filters. (B-D) Selected regions shown at ...
... and find where to buy Vintage 1973 Clos du Val Cabernet Sauvignon High Shoulder Corroded Capsule Stained Label ... Food Pairings for Clos du Val Cabernet Sauvignon High Shoulder Corroded Capsule Stained Label. * Quick-Roasted Winter ... Clos du Val Cabernet Sauvignon High Shoulder Corroded Capsule Stained Label 1973 Add winemakers notes ...
Local action of intrahypophyseal implants of estrogen as revealed by staining with peroxidase-labeled antibody Supported in ... as revealed by staining with peroxidase-labeled antibody, was studied in female rats. Pellets of pure cholesterol and of ... "Local action of intrahypophyseal implants of estrogen as revealed by staining with peroxidase-labeled antibody Supported in ... cells assumed to be responsible for luteinizing hormone secretion were reduced in size and stained more intensely; and growth ...
... the rhodamine 6G-labeled (co)polymers selectively stain the mitochondria while the fluorescein-labeled copolymers were ... A Non-toxic Cellular Staining Agent Based on Fluorescently Labeled Poly[2-(methacryloyloxy)ethyl phosphorylcholine]. N.J. ... Human dermal fibroblast cells were treated with these labeled polymers and rapid internalization and efficient cell staining ... In this study we show that PMPC alone can be used as a carrier for small molecules by covalently attaching a fluorescent label ...
... Presented at the Abcam virtual exhibition: support and products ...
Cells were stained with fluorescently labeled protein-specific antibodies, lyzed in a nonionic detergent and... ... Proteins were identified with the help of fluorescently labeled antibodies. Staining of cells with fluorescently labeled ... Cells were stained with fluorescently labeled protein-specific antibodies, lyzed in a nonionic detergent and gel-filtered on ... Study of lipid rafts by gel filtration combined with preliminary staining with fluorescently labeled antibodies. ...
Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your primary ... Mix-n-Stain™ CF532 Antibody Labeling Kit. {{ i.item.name }} Product Specifications Technical Documentation ... Mix-n-Stain™ CF532 Antibody Labeling Kit is an orange fluorescent dye with absorption and emission maxima at 527 and 558 nm, ... Mix-n-Stain™ 10X reaction buffer Mix-n-Stain™ antibody storage buffer Ultrafiltration vial ...
CellTracker Green labelling vs. rose bengal staining: CTG wins by points in distinguishing living from dead anoxia-impacted ... How to cite: Grego, M., Stachowitsch, M., De Troch, M., and Riedel, B.: CellTracker Green labelling vs. rose bengal staining: ... Generally, RB will stain dead but not yet decomposed copepods and nematodes equally as it does live ones. Specifically, RB ... As already accepted for foraminiferal research, we demonstrate that the CTG labelling is also valid for other meiofauna groups. ...
Microbial detection in microfluidic devices through dual staining of quantum dots-labeled immunoassay and RNA hybridization. ... Microbial detection in microfluidic devices through dual staining of quantum dots-labeled immunoassay and RNA hybridization. ... solution and QDs-labeled antibody solution were sequentially pumped into the device to accelerate the fluorescent labeling ... labeled immunofluorescent assay (IFA). The coin sized device consists of a microchannel and filtering pillars (gap = 1-2 μm) ...
Metabolic Labeling.. Transfected 293T cells were metabolically labeled with Tran-35S-label (ICN) at a concentration of 100 μCi/ ... Immunofluorescence Staining.. HeLa cells were grown on glass coverslips in growth medium. Cells were transfected by using the ... followed by incubation with dichlorotriazinyl aminofluorescein-labeled anti-mouse Ig (Chemicon) or Cy3-labeled anti-rabbit Ig ( ... the cells were fixed and stained with anti-p65/RelA to examine the subcellular distribution of p65/RelA (Left), with anti-Flag ...
"Staining and Labeling" by people in this website by year, and whether "Staining and Labeling" was a major or minor topic of ... "Staining and Labeling" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Staining and Labeling" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Staining and Labeling". ...
Prussian Blue Staining and TEM Images of SPIO-labeled Cells. No decrease in viability was observed in labeled cells incubated ... C) Iron staining using Prussian blue. (D) TEM image of cells labeled with 200 µg Fe ml−1 SPIO. Arrows indicate the SPIOs in the ... After labeling with SPIO (5-200 µg Fe ml−1) for 24 h, the unlabeled and labeled cells were incubated with medium containing 2.5 ... SPIO labeling (200 µg Fe ml−1) induced a significant increase in ERK1/2 and AKT phosphorylation (labeled vs unlabeled, p , 0.05 ...
Retrograde labeling surgeries. All retrograde labeling surgeries were performed at P21, in WT and UCHL1-eGFP mice. After ... Immunocytochemistry and cellular staining. The antibodies were purchased from Millipore unless otherwise stated. Antibodies ... CSMN labeling.. CSMN were retrogradely labeled by injection of either red fluorescent microspheres (LumaFluor) or Fluoro-Gold ( ... To determine the percentage of SMN that are genetically labeled with eGFP expression, we performed eGFP and ChAT co-labeling ...
Stain on America! Trump denounces Fake News Media after string of major reporting errors exposed ... The proposed regulation would require store owners to label prepared, unpackaged foods found in salad bars and food bars, soups ... Supermarket owners argue a pending federal food-labeling rule that stems from the new health care law would overburden ... The FDA says much of ObamaCare is aimed at helping Americans live healthier lives, and these proposed labeling requirements ...
EdU administration, labeling, and staining. For cumulative EdU (5-ethynyl-2′-deoxyuridine, Invitrogen) labeling [44], mice ... EdU labeling. d No CD45+-cells (red, arrowhead) in the retina were observed to be labeled by EdU (green, arrow) in mice of ... These weakly stained TH+-cell bodies were never observed in retinas of Ranbp2 +/− mice. c The increase of the thickness of the ... c Only few GFAP+-cells (red) in the retina were co-labeled by EdU (green) in wild-type mice; GFAP+-cells are shown with (arrow ...
Label Information. Ingredients from packaging:. Helianthus Annuus (Sunflower) Seed Oil, Ricinus Communis (Castor Seed) Oil, ... We assess the ingredients listed on the labels of personal care products based on data in toxicity and regulatory databases, ... EWG scientists reviewed tarte cheek stain, Natural Beauty (old formulation) for safety according to the methodology outlined in ... EWGs rating for tarte cheek stain, Natural Beauty (old formulation) is 4. ...
We assess the ingredients listed on the labels of personal care products based on data in toxicity and regulatory databases, ... EWG scientists reviewed Maias Mineral Galaxy Lip n Cheek Stain, Grace for safety according to the methodology outlined in our ... Label Information. Ingredients from packaging:. Euphorbia Cerifera (Candelilla) Wax, Copernicia Cerifera (Carnauba) Wax, ... Maias Mineral Galaxy Lip n Cheek Stain, Grace. See all: Maias Mineral Galaxy ...
Fluorescence labeling of both strains with two fluorescent proteins, m-Cherry and Green Fluorescent Protein, was achieved by ... Fluorescence labeling of both strains with two fluorescent proteins, m-Cherry and Green Fluorescent Protein (GFP), was achieved ... these biofilms were also stained with crystal violet. Additionally, the fluorescence-labeled bifidobacterial biofilms ( ... "non-labeled, non-ropy" strain (or "fluorescence-labeled, non-ropy" vs. "non-labeled, ropy") for adhesion to HT29 were carried ...
Western blotting using in-gel protein labeling as a normalization control: Stain-free technology. Methods in Molecular Biology ... Fingerprint Dive into the research topics of Western blotting using in-gel protein labeling as a normalization control: Stain- ... Gilda, J. E., & Gomes, A. V. (2014). Western blotting using in-gel protein labeling as a normalization control: Stain-free ... Gilda, Jennifer E. ; Gomes, Aldrin V. / Western blotting using in-gel protein labeling as a normalization control : Stain-free ...
These are used as probes, stains, markers, NIR-labels, pH-sensors, ion indicators, chelators and in other applications by life- ... stains and labels. Chemodex is an expert in the synthesis of fluorescent substances derived from fluorophores such as coumarin ... Dil Stain. CDX-D0230. Lipophilic orange-red fluorescent membrane stain that diffuses laterally to stain the entire cell. The ... Stains/Labels/Dyes. - Markers & Probes. - Metabolites. - Building Blocks & Small Molecules. Chemodex is an expert in the ...
  • High quality Stains, Dyes, Fluorescent Probes and Labels are available for use in various immunoassays including Flow Cytometry, Immunofluorescense, Immunohistochemistry, and other applications. (scbt.com)
  • Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. (biotium.com)
  • CF™dyes are highly water soluble, small organic dyes designed for labeling proteins and nucleic acids. (goldbio.com)
  • Widely used cationic bluish-green dyes for both light and electron microscopy to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. (adipogen.com)
  • Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes. (fishersci.com)
  • Our flagship Alexa Fluor® dyes provide superior fluorescence that spans the near-UV, visible, and near-IR spectra for protein and nucleic acid labeling. (thermofisher.com)
  • Our SYTO® dyes permeate both living and dead cells, and are used to detect microbes as well as to stain mammalian cells. (thermofisher.com)
  • We also provide standard generic stains, including high-purity Hoechst dyes, propidium iodide, and others. (thermofisher.com)
  • We also have a full portfolio of dyes that are sensitive to the relative hydrophobicity or hydrophilicity of their environment, making them ideal for staining cell membranes and hydrophobic surfaces. (thermofisher.com)
  • No dual labelling with these two dyes was reported hitherto in the literature. (biomedcentral.com)
  • The staining of biological cells and tissues with dyes, especially fluorochromic dyes, in order to differentiate one from another or simply to render them more easily observable under a microscope or other sensing means is well known in the art. (google.com)
  • The AnaTag kits are designed for use with the company's dyes for labeling inas little as 30 min. (photonics.com)
  • We also offer a wide range of high quality fluorophores for easy labeling of diverse biomolecules including proteins, antibodies, and DNA, as well as complete labeling kits and ready-to-use fluorescent conjugates. (promocell.com)
  • Cells were stained with fluorescently labeled protein-specific antibodies, lyzed in a nonionic detergent and gel-filtered on Sepharose 4B. (springer.com)
  • Proteins were identified with the help of fluorescently labeled antibodies. (springer.com)
  • Staining of cells with fluorescently labeled antibodies prior to cell lysis significantly simplified the identification procedure. (springer.com)
  • Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines. (fishersci.com)
  • Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin No-Weigh™ Format is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules. (fishersci.com)
  • Determine biotinylation levels in labeled antibodies and other proteins using this fluorescent assay reagent and HABA dye for rapid and sensitive measurement. (fishersci.com)
  • To reduce background staining in IHC, ICC and other immunostaining methods, samples are incubated with a buffer that blocks the reactive sites to which the primary or secondary antibodies may otherwise bind. (wikipedia.org)
  • Methods to eliminate background staining include dilution of the primary or secondary antibodies, changing the time or temperature of incubation, and using a different detection system or different primary antibody. (wikipedia.org)
  • Finding Alexa Fluor® labeled mouse, goat and rabbit secondary antibodies for immunocytochemistry and immunohistochemistry applications on Zeiss, Leica, Olympus and Nikon microscope systems. (apple.com)
  • AnaSpec Inc. has launched a line of protein-labeling kits for primary and secondary antibodies. (photonics.com)
  • Mix-n-Stain™ CF532 Antibody Labeling Kit is an orange fluorescent dye with absorption and emission maxima at 527 and 558 nm, respectively. (goldbio.com)
  • Mix_n_Stain CF405M Antibody Labeling Kit antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Mix_n_Stain CF405M Antibody Labeling Kit. (antibody-antibodies.com)
  • Stimulation of β 1 -AR, but not β 2 -AR, markedly induced myocyte apoptosis, as indicated by increased terminal deoxynucleotidyltransferase-mediated UTP end labeling or Hoechst staining positive cells and DNA fragmentation. (pnas.org)
  • The cells were cultured at different CdCl 2 concentrations and for different time periods followed by staining of the infected red blood cells with Fluo-3/AM for Cd detection and Hoechst 33342 for parasite DNA labelling. (biomedcentral.com)
  • In addition, confocal microscopy overlay of Fluo-3/AM and Hoechst staining shows that the Cd metal accumulates in the parasite itself. (biomedcentral.com)
  • This was achieved by dual labelling with Fluo-3/AM which labels the heavy metal whose transport is studied and Hoechst which labels the parasite by binding to its DNA. (biomedcentral.com)
  • The mitochondria (left image, and red in right image) and the nuclei (blue in right image) of live HeLa cells were stained using Mitochondrial Stain and Hoechst nuclear stain, respectively. (activemotif.com)
  • To selectively stain the nuclei of the cells, the blue fluorescent Hoechst stain is also included in the kit. (activemotif.com)
  • To visualize the mitochondria in combination with the Hoechst nuclear stain, a commonly used Cy3 filter set (550-580 nm/590-650 nm) will separate the fluorescent spectra. (activemotif.com)
  • To detect the Hoechst nuclear stain, a standard DAPI filter set (370-410 nm/435-485 nm) can be used. (activemotif.com)
  • Other nuclear stains used to visualize DNA during interphase and mitosis include Hoechst stain, ethidium bromide, propidium iodide and acridine orange. (news-medical.net)
  • PromoCell provides a variety of reagents for staining different cell organelles and structures as well as for determining cellular processes like membrane potential changes or apoptosis. (promocell.com)
  • Cellular labeling with CellLight™ reagents employs BacMam technology, which uses a modified insect cell baculovirus coupled with a mammalian promoter as a vehicle to efficiently deliver and express genes in mammalian cells. (thermofisher.com)
  • The inability of baculoviruses to replicate in mammalian cells renders them safe as research reagents and provides a transient, footprint-free method to label cells. (thermofisher.com)
  • CellLight™ reagents provide a simple and effective method for introducing targeted intracellular labels into live cells, enabling unambiguous visualization by fluorescence microscopy of key organelles involved in membrane trafficking. (thermofisher.com)
  • The reagents are used as probes, stains, markers, NIR-labels, pH-sensors, ion indicators, chelators and in other applications (e.g. analytical biochemistry, immunoassays and microscopy) by life science researchers in academia, biotechnology and the diagnostics & pharmaceutical industry. (adipogen.com)
  • One of the most popular green fluorescent reagents used for click chemistry labeling of peptides, proteins, nucleotides or even small molecules. (adipogen.com)
  • This contribution of more than 35 years of experience, knowledge, and intellectual property has generated a diverse portfolio of products and applications such as immunoassay reagents, protein and nucleic acid microarray labeling kits, drug discovery assays, precast gels, RNAi conjugates, next-generation sequencing kits, real-time PCR master mixes, and more. (thermofisher.com)
  • In addition to mitochondria, the kit stains intracellular membranes and nucleoli when the cells are first treated with fixation reagents. (activemotif.com)
  • Kit for biotin-labeling of DNA by PCR. (promocell.com)
  • Biotin-16-UTP conjugate (10 mM) for RNA labeling by in vitro transcription. (promocell.com)
  • Depending on the tissue type and the method of antigen detection, endogenous biotin or enzymes may need to be blocked or quenched, respectively, prior to antibody staining. (wikipedia.org)
  • Discovering which Invitrogen, Gibco or Ambion cell health, cell culture, or reactive dye kits are best for benchtop cytometry, tissue staining, or biotin streptavidin conjugation for microscopic labeling and detection. (apple.com)
  • This study describes a modified biotin labelling/osmotic lysis method which rapidly produces membrane extracts enriched for labelled surface antigens and also improves the efficiency of antigen recovery compared with traditional detergent extraction and surface radio-iodination. (biomedcentral.com)
  • After surface labelling with biotin in the presence of the inhibitor furosemide, detergent extraction and osmotic lysis methods of enriching for the membrane fractions were compared to determine the efficiency of purification and recovery. (biomedcentral.com)
  • Biotin-labelled proteins were identified on silver-stained SDS-polyacrylamide gels. (biomedcentral.com)
  • Detergent extraction and osmotic lysis were compared for their capacity to purify biotin-labelled Plasmodium falciparum and Plasmodium chabaudi erythrocyte surface antigens. (biomedcentral.com)
  • Therefore the studies were carried out using a biotin labelling/osmotic lysis method that can rapidly produce membrane extracts enriched for labelled surface antigens. (biomedcentral.com)
  • The N-hydroxysuccinimide (NHS) ester vitamin biotin (sulpho-NHS-LC-biotin) reacts efficiently with primary amino groups and primarily labels lysine residues and the N-termini of proteins. (biomedcentral.com)
  • However in this study it was not clear whether sulpho-NHS-LC-biotin can efficiently label PESAs. (biomedcentral.com)
  • This study demonstrates that the exposure of intact IEs to sulpho-NHS-LC-biotin in the presence of furosemide and the extraction of labelled proteins by osmotic lysis provides an alternative to traditional radioisotope labelling and detergent-based protein extraction methods. (biomedcentral.com)
  • Western blotting, immunohistochemistry, and terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling were conducted at 24 hours after MCAO. (ahajournals.org)
  • FTY720 significantly retained Akt and extracellular signal-regulated kinase phosphorylation and Bcl-2 expression and decreased cleaved caspase-3 expression and terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling-positive neurons at 24 hours after MCAO. (ahajournals.org)
  • PlusOne Silver Staining Kit, Protein and PlusOne DNA Silver Staining Kit are two silver staining kits that bring speed, convenience, and very high sensitivity to the staining of proteins and nucleic acids in polyacrylamide electrophoresis. (thomassci.com)
  • Brings speed, convenience and very high sensitivity to the staining of proteins and nucleic acids. (thomassci.com)
  • Depending on the nature of the fluorescent label, organelle specificity was also observed: the rhodamine 6G-labeled (co)polymers selectively stain the mitochondria while the fluorescein-labeled copolymers were distributed throughout the cellular matrix. (techconnectworld.com)
  • Fluorescent nucleic acid stain useful for staining DNA, chromosomes, nuclei and mitochondria. (adipogen.com)
  • In live cells it stains mitochondria with low background and little or no staining of other organelles. (activemotif.com)
  • Our highly sensitive nucleic acid stains have set the standard for detecting and quantitating RNA, DNA or oligonucleotides in gels, cells, solutions, and in capillary and flow cytometry applications. (thermofisher.com)
  • Our nucleic acid stains are useful in a wide variety of applications including imaging, flow cytometry, and microplate assays. (thermofisher.com)
  • In addition to showing that this vector is suitable for flow cytometry and fluorescence microscopy, they also show that this method is compatible with dead cell staining using propidium iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. (addgene.org)
  • DAPI can be used to stain nuclei using fluorescent microscopy, flow cytometry, or to stain chromosomes in fluorescence in situ hybridization (FISH). (news-medical.net)
  • The kits are available in two reaction sizes - microscale (200 μg × 3 reactions or 100 μg × 5 reactions) and larger scale (5 mg × 3 reactions) - and are suitable for immunofluorescent staining, for fluorescence in situ hybridization and for flow cytometry. (photonics.com)
  • Fluorescent label for amine modification and protein conjugation. (adipogen.com)
  • label -OH by urethane bonds for conjugation to cysteines by thioether bonds. (fishersci.com)
  • The labeling and identification of long-range axonal inputs from multiple sources within densely reconstructed electron microscopy (EM) datasets from mammalian brains has been notoriously difficult because of the limited color label space of EM. (elifesciences.org)
  • b ) A brain tissue sample containing fluorescently labeled axons is volume imaged first using confocal laser scanning microscopy (LM) and then using 3D electron microscopy (EM). (elifesciences.org)
  • DAPI, or 4,6-diamidino2-phenylindole, is a fluorescent nucleic acid dye used to preferentially stain double-stranded DNA. (news-medical.net)
  • DAPI specifically stains double stranded DNA with no non-specific labeling in the cytoplasm. (news-medical.net)
  • To the start the staining process, first a stock solution of DAPI needs to be prepared. (news-medical.net)
  • For the staining, add the diluted DAPI stock solution to pelleted cells. (news-medical.net)
  • This can reduce the signal to noise ratio when using fluorescent stains such as DAPI, which can make clear visualization and interpretation of images difficult. (news-medical.net)
  • A) ADSC labeled with EdU at 0, 10, 20, and 50 μM and stained with Alexa-594 (red fluorescence) and DAPI (blue fluorescence). (nih.gov)
  • Major organs and tissues were harvested at 7h, 2w, 4w, and 6w and stained with Alexa-594 (red fluorescence) and DAPI (blue fluorescence). (nih.gov)
  • The Alexa-594 and DAPI stained images were digitally merged. (nih.gov)
  • Here, we report FluoEM for the identification of multi-color fluorescently labeled axons in dense EM data without the need for artificial fiducial marks or chemical label conversion. (elifesciences.org)
  • The fluorescently labeled axons (red and green) contained in the LM dataset are then matched to the corresponding axons in the EM dataset (black and white) solely based on structural constraints (see b-d), without chemical label conversion or artificial fiducial marks. (elifesciences.org)
  • over which fluorescently labeled axons (green and red) in a volume LM dataset are clearly reconstructable depends mostly on the labeling density in the respective fluorescence channel. (elifesciences.org)
  • An indirect fluorescent antibody staining procedure for detection of Vibrio cholerae serovar 01 cells in aquatic environmental samples. (currentprotocols.com)
  • It has a strong blue fluorescence and can be used to create blue fluorescent bioconjugates or labeling synthetic peptides for high-throughput detection. (adipogen.com)
  • Common loading controls include housekeeping proteins, such as β-actin or GAPDH, quantified by Western blot, or total protein, quantified using a stain such as Coomassie Brilliant Blue or Ponceau S. A more recently developed method for total protein quantification utilizes stain-free technology, which has a linear dynamic detection range and allows for protein detection on both gels and membranes. (elsevier.com)
  • Your company can capitalize on this product and intellectual property portfolio, knowledge, and experience base in order to fulfill your development and supply needs for commercial-use fluorescent labeling and detection. (thermofisher.com)
  • The stain-free technology is able to detect total protein in a large linear dynamic range and has the advantage of allowing protein detection on the gel before transblotting. (elsevier.com)
  • A great amount of non-specific binding causes high background staining which will mask the detection of the target antigen. (wikipedia.org)
  • DNA Silver Staining Kit provides reproducible and sensitive nonradioactive detection of nucleic acids in polyacrylamide gels in less than 1.5 h. (thomassci.com)
  • The detection of EdU label requires no harsh treatment or immunologic reaction, as detection of BrdU label does. (nih.gov)
  • Can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. (adipogen.com)
  • Here, we describe the theory and use of stain-free gels for total protein quantification and normalization of Western blots. (elsevier.com)
  • Our fluorogenic SYPRO® protein stains can be used for electrophoretic slab gels as well as for capillary electrophoretic separation of proteins, and for detecting proteins on surfaces. (thermofisher.com)
  • This chapter discusses the theory, advantages, and method used to do total protein quantification using stain-free gels for normalization of Western blots. (elsevier.com)
  • Herein, we present a new approach, virtual staining, that improves the Raman spectral histopathology (SHP) images of colorectal cancer tissue by combining the integrated Raman intensity image in the C-H stretching region (2800-3050 cm −1 ) with the pseudo-colour Raman image. (rsc.org)
  • The virtual staining image also represents the whole biochemical fingerprint, and several tissue components including carcinoma were identified automatically with high sensitivity and specificity. (rsc.org)
  • Applications for slide assays include staining and imaging of tissue slices representing various diseased and normal states, performing cell-based assays in array format, and testing assays already developed on slides prior to moving to plate-based formats. (moleculardevices.com)
  • The ImageXpress® Screening Systems make selective slide scanning easy, letting you quickly create automated workflows for scanning and high-resolution imaging of regions of interest on either histologicallystained or fluorescently-labelled tissue. (moleculardevices.com)
  • The approach is based on correlated tissue imaging and computational matching of neurite reconstructions, amounting to a virtual color labeling of axons in dense EM circuit data. (elifesciences.org)
  • These multicolor labeling and tissue clearing techniques are very helpful for studying the brain. (elifesciences.org)
  • Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish. (nih.gov)
  • Human artery wall cross-section, elastic tissue stain. (gettyimages.com)
  • BrdU) has been used widely to label cells in culture and in tissue. (nih.gov)
  • In the present study we tested a new thymidine analog, 5-ethynyl-2-deoxyuridine (EdU), for labeling and tracking of mesenchymal stromal cells (MSC), specifically adipose tissue-derived stem cells (ADSC). (nih.gov)
  • An improved method to determine cell viability by simultaneous staining with fluorescein diacetate‐propidium iodide. (currentprotocols.com)
  • Covalently labeling of the surface of live cells, withstanding fixation and permeabilization for subsequent immunofluorescence staining. (promocell.com)
  • Cell types previously not compatible with or poorly transduced with BacMam 1.0 can now be labeled with BacMam 2.0, including primary cells (e.g., neurons) and stem cells. (thermofisher.com)
  • The direct action of 17 Β-estradiol and cholesterol on cells of the pituitary pars distalis, as revealed by staining with peroxidase-labeled antibody, was studied in female rats. (umich.edu)
  • Human dermal fibroblast cells were treated with these labeled polymers and rapid internalization and efficient cell staining was achieved within one hour, as judged by confocal microscopy. (techconnectworld.com)
  • Labeling Stem Cells with a New Hybrid Bismuth/Carbon Nanotube Contrast Agent for X-Ray Imaging. (viictr.org)
  • INS-1 cells were labeled in culture medium containing clinically approved SPIO for 24 h. (wiley.com)
  • Labeled and unlabeled cells were stimulated with glucose (25 mM) or insulin (0.1-1 µM) for 12 h. (wiley.com)
  • This study demonstrates that SPIO labeling alters some fundamental functional variables, at least in INS-1 cells, through modulation of the glucose- or insulin-induced activation of ERK1/2 and AKT, which leads to insulin biosynthesis. (wiley.com)
  • We develop a label-free optical technique to image and discriminate undifferentiated human embryonic stem cells (hESCs) from their differentiating progenies in vitro . (spiedigitallibrary.org)
  • Our SYTOX® stains provide a clear indication of dying or compromised cells because they are relatively non-fluorescent until binding nucleic acids and they are membrane impermeant, so they only reach the nucleus of dead cells. (thermofisher.com)
  • In wild-type mice, proliferating cell nuclear antigen (PCNA)-positive cells in the matrigel were significantly less in diabetic than in nondiabetic mice, while the numbers of transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells were significantly higher. (diabetesjournals.org)
  • Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. (wikipedia.org)
  • Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just a fraction of a genetically-defined subset of cells. (addgene.org)
  • By labeling fewer cells in a population, it's easier to visualize individual/non-overlapping cells. (addgene.org)
  • By truncating one of the recombination sites in the construct to different lengths, the density of cells that are labeled can be altered to dense (45% of cells labeled), intermediate (12%), and sparse (4%) levels. (addgene.org)
  • Figure 3: Micrographs of wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate cell death. (addgene.org)
  • Furthermore, in contrast to the use of radioactive labelled heavy metal, the fluorescent labelling enables us to differentiate between the different populations existing in a P. falciparum infected red blood cells cultures and thus actually study a phenomenon at the level of a single cell. (biomedcentral.com)
  • In vitro MRI showed that 1 × 10 6 cells labeled with various concentrations of MIRB (12.5-100 μ g/mL) could be visualized. (hindawi.com)
  • To distinguish specific cells using MRI, transplanted cells must be labeled with a magnetic contrast agent. (hindawi.com)
  • The Chromeo™ Live Cell Mitochondrial Staining Kit utilizes a proprietary, non-toxic, water-soluble, membrane-permeable dye that rapidly diffuses into live or fixed cells. (activemotif.com)
  • When fixed cells are stained, internal membranes and nucleoli become additional targets of the dye. (activemotif.com)
  • Because of the excellent retention of the stain in the cell and its low toxicity, it is an ideal tool for long-term labeling of cells and cellular tracking . (activemotif.com)
  • Figure 1: Staining of living HeLa cells with Chromeo™ Live Cell Mitochondrial Staining Kit. (activemotif.com)
  • This long Stokes shift separates the fluorescence of the stain from any auto-fluorescence background of living cells. (activemotif.com)
  • VCAM-1 was not detected on intimal endothelial cells, but strong staining of adventitial lymphoid aggregates for this molecule was seen. (nih.gov)
  • O) Higher magnification image of c233 highlighting ventral cells shown in F. (P) c223 also exhibits labeling of the exocrine pancreas at 5 dpf (earlier expression, not shown). (nih.gov)
  • Proliferation of murine T lymphocytes in blood, lymph nodes, and spleen was studied in four in vivo stimulation systems, using BrdU pulse-labeling of DNA-synthesizing cells. (jimmunol.org)
  • This technique can locate cycling cells and was preferred to continuous BrdU administration which cumulatively labels the progeny of cycling precursors ( 11 , 12 ), T cells labeled at the ultimate step of intrathymic cell maturation ( 13 ), and cycling peripheral cells. (jimmunol.org)
  • 2. The method of claim 1 wherein the cells are fixed prior to staining. (google.com)
  • 3. The method of claim 1 wherein the cells are vitally stained. (google.com)
  • More particularly, the present invention relates to the use of pyrylium and thiapyrylium compounds as stains for biological cells and tissues. (google.com)
  • White cells are distinguished by detecting the resultant green fluorescence emitted by the stained nuclei of the leucocytes and differentiated by the amplitude of the red fluorescence emitted. (google.com)
  • Labeling and tracking of mesenchymal stromal cells with EdU. (nih.gov)
  • The labeled cells can also be tracked when transplanted into a suitable host. (nih.gov)
  • EdU was incorporated specifically into the nucleus in approximately 50% of ADSC and the percentage of cells that remained fully labeled declined with time. (nih.gov)
  • E) Nuclei of cells labeled with EdU at 10 μM and grown for 21 days. (nih.gov)
  • Another less commonly used method is total protein normalization using stains, such as Ponceau S or Coomassie Brilliant Blue, which stains all the proteins on the blots. (elsevier.com)
  • The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation. (viictr.org)
  • PURPOSE: To develop a magnetic resonance histology (MRH) staining and fixation method by immersion to enhance the signal-to-noise ratio (SNR) with a paramagnetic contrast agent permitting microscopic acquisition within a 3-hour scan time. (duke.edu)
  • Fixation/staining was performed by immersion in Bouin's fixative containing varied concentrations of ProHance (from 10:1 to 500:1 Bouin's:ProHance) and for varied immersion durations (up to 24 hours). (duke.edu)
  • We compared two methods: CellTracker Green (CTG) - new labelling approach for meiofauna - with the traditional rose bengal (RB) staining method. (biogeosciences.net)
  • Gilda, JE & Gomes, AV 2014, ' Western blotting using in-gel protein labeling as a normalization control: Stain-free technology ', Methods in Molecular Biology , vol. 1295, pp. 381-391. (elsevier.com)
  • Widely used to label peptides, proteins, drugs and other biomolecules. (adipogen.com)
  • Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent. (fishersci.com)
  • Easily and gently label (iodinate) proteins and peptides with iodine-125 using reagent-coated polystyrene beads that can be removed following reaction. (fishersci.com)
  • The Tol2-mediated gene/enhancer trapping system together with UAS transgenic lines provides valuable tools for regulated gene expression and for targeted labeling and ablation of specific cell types and tissues during early zebrafish development. (nih.gov)
  • Labeling of various organs/tissues was done by intraperitoneal injection of EdU and examined by histology and fluorescence microscopy. (nih.gov)
  • Tracking of ADSC was done by intratissue or intravenous transplantation of EdU-labeled ADSC into various tissues and examined by histology and fluorescence microscopy. (nih.gov)
  • EdU labeling of tissues. (nih.gov)
  • Convenient and sensitive cell staining with low cytotoxicity. (promocell.com)
  • Lipophilic orange-red fluorescent membrane stain that diffuses laterally to stain the entire cell. (adipogen.com)
  • Used for labeling of cell-surface functional groups (glycophorins). (adipogen.com)
  • Pancreatic islets, as well as insulinoma cell lines ( β -TC3, β -TCtet and β -TC-6) that possess the ability to synthesize and secrete insulin, have been labeled with clinically approved and commercially available SPIOs without decreasing cell viability or hindering insulin secretion [ 1-3, 12-17 ]. (wiley.com)
  • Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling. (fishersci.com)
  • MIRB labeled hDPSCs cell can be visualized by MRI in vitro and in vivo. (hindawi.com)
  • In addition to serving as a mitochondrial stain, the Chromeo™ Live Cell Mitochondrial Stain can be used as a long-term cell label. (activemotif.com)
  • The Chromeo™ Live Cell Mitochondrial Staining Kit features a unique dye that is not fluorescent until after it has reacted with an amino group, which induces a structural change that results in bright red fluorescence. (activemotif.com)
  • Chromeo™ Live Cell Mitochondrial Stain can be excited between 470 nm and 550 nm (maximum at 503 nm), enabling the use of common lasers or other light sources. (activemotif.com)
  • The number of experiments will depend on the chosen concentration of the stain, which may vary between different cell types. (activemotif.com)
  • These data appear to conflict with those of other reports in which lymphocyte turnover rates were calculated on the basis of cell number dynamics ( 5 , 6 , 7 ), and during SIV infection in macaques studied by means of continuous BrdU labeling for 3 wk ( 8 ). (jimmunol.org)
  • Cell labeling with EdU in culture or living animals can be performed easily. (nih.gov)
  • Selective in vivo labeling of neuron populations that degenerate in diseases is a primary step toward understanding mechanisms responsible for their vulnerability. (jneurosci.org)
  • Brighter fluorescent labeling colors would allow scientists to use high-powered microscopes to trace the entire length of a neuron in a whole brain much more quickly and easily. (elifesciences.org)
  • It is still difficult, however, to dissect and trace an individual neuron from a brain sample labeled with a single type of fluorescent protein. (elifesciences.org)
  • Mouse-brain slice stained by Immunohistochemistry. (wikipedia.org)
  • In particular, fluorescent proteins are useful for labeling genetically-defined neuronal populations. (elifesciences.org)
  • Biotinylation and osmotic lysis provides an improved method to label and purify parasitised erythrocyte surface antigen extracts from both in vitro and ex vivo Plasmodium parasite preparations. (biomedcentral.com)
  • In this study we show that PMPC alone can be used as a carrier for small molecules by covalently attaching a fluorescent label. (techconnectworld.com)
  • The labeling procedure tolerates many common buffer components and antibody stabilizers. (biotium.com)
  • This is then incubated for around 5 minutes, and then rinsed in PBS few times to remove excess stain and buffer from the sample. (news-medical.net)
  • The sample should be fixed appropriately and rinsed in deionized water to remove residual buffer and non-specific staining. (news-medical.net)
  • The study of the Plasmodium falciparum heavy metal transporter gene pfmdr2 employed radioactive labelled heavy metal. (biomedcentral.com)
  • After labeling with increasing amounts of SPIO, cytotoxicity was not observed, yet the intracellular iron concentration increased in a dose-dependent manner. (wiley.com)
  • We also developed a Tetbow system with chemical tags, in which genetically encoded chemical tags were labeled with synthetic fluorophores. (elifesciences.org)
  • EWG scientists reviewed tarte cheek stain, Natural Beauty (old formulation) for safety according to the methodology outlined in our Skin Deep Cosmetics Database. (ewg.org)
  • EWG's rating for tarte cheek stain, Natural Beauty (old formulation) is 4. (ewg.org)
  • EWG scientists reviewed Maia's Mineral Galaxy Lip n Cheek Stain, Grace for safety according to the methodology outlined in our Skin Deep Cosmetics Database. (ewg.org)
  • EWG's rating for Maia's Mineral Galaxy Lip n Cheek Stain, Grace is 1. (ewg.org)
  • Poor at removing blood stains. (consumer.org.nz)
  • Getting blood stains out of fabric is a challenge, and it's always easier to address stains when they're fresh if possible. (wikihow.com)
  • For monitoring and biodiversity studies, the RB method might be sufficient, but for more precise quantification of community degradation, especially after an oxygen depletion event, CTG labelling is a better tool. (biogeosciences.net)
  • In the present study, we developed a stochastic multicolor labeling method with enhanced expression levels that uses a tetracycline-operator system (Tetbow). (elifesciences.org)
  • have developed a bright multicolor labeling method for neurons called Tetbow. (elifesciences.org)
  • The dual fluorescent labelling is a valid method for detecting heavy metal accumulation in P. falciparum . (biomedcentral.com)
  • Included are our SYBR® Green I stain, which has proven to be the ubiquitous choice by researchers around the world for real-time PCR and PicoGreen® reagent, which is the most commonly used reagent for quantifying double stranded DNA. (thermofisher.com)
  • [1] [5] These steps may make the difference between the target antigens staining or not staining. (wikipedia.org)