A simple hydroponic culture method for the development of a highly viable root system in Arabidopsis thaliana. (1/7660)

In the studies of nutritional absorption and metal toxicity in the root, it is important to grow plants without technical damage. We established a simple hydroponic culture system for Arabidopsis thaliana to obtain a healthy plant having a well-developed root system with many lateral roots. The phytotoxic effects of Cr, Cu, and Al ions were examined by FDA-PI staining using this culture system. The pattern of root inhibition varied with the ion, suggesting the usefulness of this culture system.  (+info)

Inhibition of IL-6 and IL-8 induction from cultured rheumatoid synovial fibroblasts by treatment with aurothioglucose. (2/7660)

Gold compounds have long been used in the treatment of rheumatoid arthritis (RA). However, their actions in RA have not been clarified. In this study, we examined the effect of one of the monovalent gold compounds, aurothioglucose (AuTG), on the IL-1-induced production of IL-6, IL-8 and granulocyte macrophage colony stimulating factor (GM-CSF) from rheumatoid synovial fibroblasts (RSF) isolated from three RA patients. IL-6 and IL-8 induction but not GM-CSF induction was inhibited in most of the RSF after pretreatment with AuTG. Since gene expression of these cytokines is known to be under the control of a common transcription factor, NF-kappaB, the effect of AuTG on the cellular localization of NF-kappaB (p65 subunit) and on NF-kappaB-DNA binding was examined. Although AuTG treatment did not prevent NF-kappaB nuclear translocation, AuTG blocked the DNA-binding activity of NF-kappaB when examined in vitro. Morphologically, both metal-specific cell staining using p-dimethylaminobenzylidene rhodamine and transmission electron microscopic examinations demonstrated the accumulation of metal gold in the cytoplama and some organella (mitochondria and lysosomes) of the AuTG-treated RSF. These results indicate that one of the anti-rheumatic actions of AuTG might be through its inhibitory action on NF-kappaB.  (+info)

Murine cytomegalovirus immediate-early promoter directs astrocyte-specific expression in transgenic mice. (3/7660)

Murine cytomegalovirus (MCMV), which causes acute, latent, and persistent infection of the natural host, is used as an animal model of human cytomegalovirus (HCMV) infection. Transcription of MCMV immediate-early (IE) genes is required for expression of the early and late genes and is dependent on host cell transcription factors. Cell-type-specific expression activity of the MCMV IE promoter was analyzed in transgenic mice generated with the major IE (MIE) enhancer/promoter involving nucleotides -1343 to -6 (1338 bp) connected to the reporter gene lacZ. Distinct expression was observed in the brain, kidneys, stomach, and skeletal muscles. Weak expression was observed in a portion of the parenchymal cells of the salivary glands and pancreas, and expression was hardly detected in the lungs, intestine, or immune and hematopoietic organs such as the thymus, spleen, lymph nodes, and bone marrow. The spectrum of organs positive for expression was narrower than that of the HCMV MIE promoter-lacZ transgenic mice reported previously and showed a greater degree of cell-type specificity. Interestingly, astrocyte-specific expression of the transgene was observed in the brain and primary glial cultures from the transgenic mice by combination of beta-galactosidase (beta-Gal) expression and immunostaining for cell markers. However, the transgene was not expressed in neurons, oligodendroglia, microglia, or endothelial cells. Furthermore, the beta-Gal expression in glial cultures was stimulated significantly by MCMV infection or by addition of calcium ionophore. These observations indicated that expression activity of the MCMV IE promoter is strictly cell-type specific, especially astrocyte-specific in the brain. This specific pattern of activity is similar to that of natural HCMV infection in humans.  (+info)

Tumor extension and cell proliferation in adenocarcinomas of the lung. (4/7660)

To elucidate the mechanism of tumor extension in human pulmonary adenocarcinoma, we immunohistochemically investigated the expression of cell cycle regulator proteins in 54 small adenocarcinomas less than 3 cm in diameter. The Ki-67-labeling index was significantly higher in the periphery of the tumor nodule than in the center. This proliferative potential correlated well with coexpression of cdk2 and cyclin A. p27, one of the cdk inhibitors, was highly expressed in normal bronchial epithelial cells. Peripherally located tumor cells expressed p27 at an intermediate level, but at a higher frequency and level than those in the center. Expression of p21 was also predominant in the periphery. Furthermore, the expression patterns of p21 and p27 were reciprocal. In vitro kinase assays further demonstrated higher cdk2 kinase activity in the periphery. These results suggest that: (i) within an emerging extension made up of peripherally located tumor cells, their high proliferative potential gradually wanes as their relative topographical position becomes more central in the expanding tumor; (ii) peripherally located tumor cells maintain their proliferative potential by higher cyclin A-cdk2 complex activity; and (iii) intermediate expression of p21/p27 in the peripherally located cells promotes higher cyclin A-cdk2 kinase activity, whereas high p21/p27 expression in nonneoplastic cells inhibits kinase activity.  (+info)

Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium. (5/7660)

In epidermis, it has been suggested, intercellular communication through gap junctions is important in coordinating cell behavior. The connexins, may facilitate selective assembly or permeability of gap junctions, influencing the distribution of metabolites between cells. Using immunohistochemistry, we have compared the distribution of connexins 26 and 43 with that of proliferating cells (Ki67 labeling) in normal epidermis, hyperplastic epidermis (tape-stripped epidermis, psoriatic lesions, and viral warts), and vaginal and buccal epithelia. Connexin 43 was abundant in spinous layers of all epidermal specimens and in vaginal and buccal epithelia. Connexin 26 was absent from the interfollicular and interductal epidermis of normal hair-bearing skin, and nonlesional psoriatic epidermis but present at very low levels in plantar epidermis. Connexin 26 was prominent in lesional psoriatic epidermis and viral warts and in vaginal and buccal epithelia. In three independent experiments connexin 26 appeared in a patchy intercellular distribution in the basal epidermis within 24 h of tape stripping, proceeding to more extensive distribution in basal and suprabasal layers by 48 h. The increase in connexin 26 preceded that in cell proliferation. In vaginal epithelium, buccal epithelium, and viral warts connexin 26 was restricted mainly to suprabasal, nonproliferating cells. In psoriatic lesional epidermis connexin 26 was also located mainly in suprabasal, nonproliferating cells. Connexin 26 was present in a patchy distribution in the basal layer of psoriatic lesional epidermis, but double labeling for connexin 26 and Ki67 showed that many connexin 26 positive basal cells were nonproliferative, suggesting that connexin 26 may be related to differentiation rather than to proliferation. These observations would be consistent with a role for connexin 26 containing gap junctions during both early and later stages of keratinocyte differentiation in hyperplastic epidermis and in vaginal and buccal epithelia.  (+info)

Targeted disruption of fibronectin-integrin interactions in human gingival fibroblasts by the RI protease of Porphyromonas gingivalis W50. (6/7660)

Cell surface integrins mediate interactions between cells and their extracellular matrix and are frequently exploited by a range of bacterial pathogens to facilitate adherence and/or invasion. In this study we examined the effects of Porphyromonas gingivalis proteases on human gingival fibroblast (HGF) integrins and their fibronectin matrix. Culture supernatant from the virulent strain W50 caused considerably greater loss of the beta1 integrin subunit from HGF in vitro than did that of the beige-pigmented strain W50/BE1. Prior treatment of the W50 culture supernatant with the protease inhibitor Nalpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) blocked its effects on cultured cells, indicating that this process is proteolytically mediated. Purified arginine-specific proteases from P. gingivalis W50 were able to mimic the effects of the whole-culture supernatant on loss of beta1 integrin expression. However purified RI, an alpha/beta heterodimer in which the catalytic chain is associated with an adhesin chain, was 12 times more active than RIA, the catalytic monomer, in causing loss of the alpha5beta1 integrin (fibronectin receptor) from HGF. No effect was observed on the alphaVbeta3 integrin (vitronectin receptor). The sites of action of RI and RIA were investigated in cells exposed to proteases pretreated with TLCK to inactivate the catalytic component. Use of both monoclonal antibody 1A1, which recognizes only the adhesin chain of RI, and a rabbit antibody against P. gingivalis whole cells indicated localization of RI on the fibroblasts in a clear, linear pattern typical of that seen with fibronectin and alpha5beta1 integrin. Exact colocalization of RI with fibronectin and its alpha5beta1 receptor was confirmed by double labeling and multiple-exposure photomicroscopy. In contrast, RIA bound to fibroblasts in a weak, patchy manner, showing only fine linear or granular staining. It is concluded that the adhesin component of RI targets the P. gingivalis arginine-protease to sites of fibronectin deposition on HGF, contributing to the rapid loss of both fibronectin and its main alpha5beta1 integrin receptor. Given the importance of integrin-ligand interactions in fibroblast function, their targeted disruption by RI may represent a novel mechanism of damage in periodontal disease.  (+info)

Adaptive immune response to Shigella flexneri 2a cydC in immunocompetent mice and mice lacking immunoglobulin A. (7/7660)

Shigella flexneri cydC, which is deficient in cytochrome bd, was rapidly cleared from the lungs of intranasally inoculated mice and was Sereny negative, yet it induced 93% protection against challenge with wild-type S. flexneri. Mice that lack immunoglobulin A (IgA) were fully protected, suggesting that IgA may not be required for adaptive immunity in this model system.  (+info)

CNS wound healing is severely depressed in metallothionein I- and II-deficient mice. (8/7660)

To characterize the physiological role of metallothioneins I and II (MT-I+II) in the brain, we have examined the chronological effects of a freeze injury to the cortex in normal and MT-I+II null mice. In normal mice, microglia/macrophage activation and astrocytosis were observed in the areas surrounding the lesion site, peaking at approximately 1 and 3 d postlesion (dpl), respectively. At 20 dpl, the parenchyma had regenerated. Both brain macrophages and astrocytes surrounding the lesion increased the MT-I+II immunoreactivity, peaking at approximately 3 dpl, and at 20 dpl it was similar to that of unlesioned mice. In situ hybridization analysis indicates that MT-I+II immunoreactivity reflects changes in the messenger levels. In MT-I+II null mice, microglia/macrophages infiltrated the lesion heavily, and at 20 dpl they were still present. Reactive astrocytosis was delayed and persisted at 20 dpl. In contrast to normal mice, at 20 dpl no wound healing had occurred. The rate of apoptosis, as determined by using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling, was drastically increased in neurons of ipsilateral cortex of the MT-I+II null mice. Our results demonstrate that MT-I+II are essential for a normal wound repair in the CNS, and that their deficiency impairs neuronal survival.  (+info)

Stained glass with label moulds over. Good belfry above western parapet gable. Federation Gothic style hall c. 1900s, ... The stained glass windows are some of the earliest to be made in the colony, the two in the first bay of the south wall of the ...
Wetzl, Bianca K.; Yarmoluk, Sergiy M.; Craig, Douglas B.; Wolfbeis, Otto S. (2004-10-11). "Chameleon Labels for Staining and ... It is within the "chameleon labels" class, so named due to their clear color-changing properties upon conjugation. Py-1 was ... Pyrylium-1 (Py-1) is a fluorogenic pyrylium & tetrafluoroborate salt and amine-labeling dye that is not fluorescent itself, but ... FQ CBQCA Fluorescamine Wolfbeis, Otto S (2021-08-12). "Fluorescent chameleon labels for bioconjugation and imaging of proteins ...
... or label; soil and stains; dogeared pages; underlining, highlighting, and marginalia; water damage; torn hinges, endpapers and ... Previous owners of books often signed their copies or labelled them with bookplates, and it is often not difficult to identify ...
Immunogold labeling or Immunogold staining (IGS) is a staining technique used in electron microscopy. This staining technique ... Immunogold labeling can introduce artifacts, as the gold particles reside some distance from the labelled object and very thin ... Van Laere O, De Wael L, De Mey J (1985). "Immuno gold staining (IGS) and immuno gold silver staining (IGSS) for the ... A more complex method of multi-site labeling involves labeling opposite sides of an antigenic site separately, the immunogold ...
After staining with heavy metal labels, Sjöstrand et al. noted two thin dark bands separated by a light region, which they ... They fused two cells labeled with different membrane-bound fluorescent tags and watched as the two dye populations mixed. The ...
... an INXS covers single on Australian label Stained Circles; a Sparks covers single on In The Red, which includes "Sherlock ... A companion remix album was released by Scion's Scion A/V label. In September 2013, Ooey Gooey Chewy Ka-blooey!, the long- ... Los Angeles-based independent label In The Red Records persuaded the Dirtbombs to record an LP. Collins decided that if the ... Five singles were issued in 2008: a cover of a Suicide song on the Irish label Infirmary Phonographic; ...
A label mould finishes the top of the openings. The east wall of the church has three tall arched openings. All the windows to ... The Cathedral's present day interior contains church furniture, furnishings and stained glass windows as well as a number of ... There are significant memorials located within the Cathedral's interior, including the stained glass windows memorial tablets. ... the church have stained glass. At the eaves and near the top of the parapet walls, the stonework is finished with a dentilated ...
Spectral traces for fluorescent dyes Manual for Hoechst stains An online guide to fluorescent probes and commercial labeling ... Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed ... There are nuclei staining dyes that allow for viability of cells after staining.[citation needed] Hoechst 33342 and 33258 are ... A concentration of 0.1-12 μg/ml is commonly used to stain DNA in bacteria or eukaryote cells. Cells are stained for 1-30 min at ...
Marshall, Cass (16 February 2023). "Deep Rock Galactic dev launches publishing label for Danish studios". Polygon. Retrieved 22 ... Coffee Stain announced Coffee Stain Publishing, a subsidiary that would act as the publishing entity within the Coffee Stain ... Coffee Stain Publishing, founded in February 2017. Coffee Stain Studios in Skövde, Sweden, founded in 2010 Easy Trigger in ... "Coffee Stain x Rare Earth Games". Coffee Stain Studios. Retrieved 4 April 2023. "Popular Roblox game Welcome to Bloxburg ...
In 2010, he created Stained Music, his own record label. Today, Stainer is known as one of the pioneers of the house music ... The first track they produced, "I Don't Want It to End" was picked up by the record label EMI / Universal Music Group and ... In 2012, Stainer released the single "Can't Take That Away" on the Hed Kandi label featuring the vocals of Roland Clark. As of ... "Piracy - Part 2 [Stained Music". Beatport. 31 January 2011. Retrieved 22 May 2012. "Music Canada: Gold Platinum Database - Karl ...
... a next-generation holographic microscope for 3D live cell imaging without the need for staining or labeling. The HT-1 is the ... "Holographic Microscope Allows Stain-Free 3D Imaging of Live Cells". Asian Scientist Magazine. 4 April 2016. Retrieved 1 April ... "TomoCube holotomography offers label-free imaging of cells". optics.org. Retrieved 1 April 2017. " ...
... the use of antisera to label specific antigens Ruthenium(II) tris(bathophenanthroline disulfonate), a protein dye. Vital stain ... Common variants include Wright's stain, Jenner's stain, May-Grunwald stain, Leishman stain and Giemsa stain. All are used to ... A Ziehl-Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the ... Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the ...
Fragmentation and labeling of the PCR product Hybridization and staining on the AmpliChip DNA microarray. Scanning the chip. ...
It is insoluble in organic compounds, and can be labeled by silver staining. It is called neuromelanin because of its function ...
Kim, Jae-Ha (19 February 1993). "Wall-to-Wall of Sound - Inspiral Carpets Resist the Stain of 'Manchester' Label". Weekend Plus ...
Digital holography has been used in different contexts to study cell morphology using neither staining nor labeling. This can ... Label-free high content analysis. Fluorescent high content analysis/screening has several drawbacks. Label-free alternatives ... Label-free cell cycle analysis. The phase shift induced by cells has been shown to be correlated to the cell dry mass. The cell ... Label-free viability analysis of adherent cell cultures. Digital holography has been used to study the apoptotic process in ...
Staining and labeling techniques are often used in microscopy to help identify specific structures in a cell. Staining usually ... In the case of dendritic spikes, staining and labeling are used to identify and quantify the presence of certain voltage-gated ... Labeling involves the use of fluorescence to identify specific molecules. Fluorophores, fluorescent molecules, may be directly ...
The album covers several tracks from the group's early career; some were previously released on small and independent labels, ... Stained Glass, Soma Fountains is a compilation album by the Legendary Pink Dots, issued in 1997 on Soleilmoon Records. ...
The cerebral organoid neurons can, however, form axons as shown by GFP staining. GFP labeled axons have been shown to have ... Many regions that have forebrain marker FOXG1, labeling them as regions with cerebral cortical morphology, were also positive ...
"Application of the DNA-specific stain methyl green in the fluorescent labeling of embryos". Journal of Visualized Experiments ( ... Fluorescent DNA staining is routinely used in cancer prognosis. Methyl green also emerges as an alternative stain for DNA in ... It has been used for staining cell nuclei either as a part of the classical Unna-Pappenheim stain, or as a nuclear counterstain ... Methyl green (CI 42585) is a cationic or positive charged stain, related to Ethyl Green, that has been used for staining DNA ...
Gilda JE, Gomes AV (2015). "Western blotting using in-gel protein labeling as a normalization control: stain-free technology". ... Gels are typically stained with Ethidium bromide or other nucleic acid stains such as GelGreen. Generally, a gel doc includes ... In the stain-free gel imaging procedure, tryptophan residues undergo a UV-induced interaction with trihalo chemicals in the ... Using the stain-free gel setting and automatically optimized exposure duration setting, photographing the gel can be done. ...
Reversible permeabilization of live cells for intracellular labeling". J Biomed Opt. 14 (2): 021012. doi:10.1117/1.3090448. PMC ... Supravital staining is a method of staining used in microscopy to examine living cells that have been removed from an organism ... The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with ... It differs from intravital staining, which is done by injecting or otherwise introducing the stain into the body. Thus a ...
... s along with fluorophores are widely used for staining cells for study both in vitro and in vivo. Furthermore, ... hence promising candidates for fluorescence bio imaging or cellular labeling. ...
The interior [was] fitted up with open benches of stained and varnished deal, arranged so as to rise by easy steps from the ... moulded labels, &c. The gable fronting the street is pierced with pointed arch window openings, having splayed dressings. The ... stained and varnished. Fresh air [was] admitted through the cast-iron columns that support[ed] the galleries, and through ... interior fittings [were] of Petersburg pine, wrought, stained and varnished. A platform [was] provided in the recess of the ...
These proteins can be stained with fluorescent dye labeled antibodies and detected using flow cytometry. The limit of detection ...
... is the debut album by Japanese metal band Blood Stain Child. It was released in 2002, by the Captain ... Rock label. "Silence of Northern Hell" - 3:41 "Crimson Symphony" - 4:45 "Under the Sin of Grief" - 5:42 "Legend of Dark" - 4:03 ... Blood Stain Child , Releases , AllMusic". AllMusic. Retrieved January 10, 2014. v t e (Articles with short description, Short ... Blood Stain Child albums, Pony Canyon albums, All stub articles, 2000s death metal album stubs). ...
The determination of the type of amyloid can be done by immunohisto-labeling techniques as well as immunofluorescence staining ... In this histological evaluation special stains are utilized to visualize the amyloid deposits. One such stain is Congo Red, ... The labeling of the P component can then be pictured by a gamma camera. Technetium radionuclide scans can now reliably ... Sirius red staining or electron microscopy examination can also be done. ...
Without techniques such as cell labeling, neuronal staining and careful post mortem analysis, these comparison studies would be ...
The song "Stains" peaked at No.26 on the Billboard Mainstream Rock Chart. The album title is Latin for "night air" and stems ... Nox Aeris is the second major label studio album and fifth overall by rock band Janus. The album was released on March 27, 2012 ... Stains (Acoustic) - 3:43 "JANUS 'Nox Aeris' Makes Billboard Debuts At #24 On "Heatseekers" And #33 On "Hard Music" Charts; Tour ... In Flames - 3:17 Stains - 3:36 Lifeless - 3:36 Promise To No One - 3:37 Pound Of Flesh - 3:08 Waive - 3:36 Stray - 4:24 Numb - ...
Mason did not record another album until 2003's Stained Glass Window, on the independent Twinbeat label. Mila Mason was born ... In 2003 Mason signed to the independent Twinbeat Records label and recorded the album Stained Glass Window, which included her ...
... a new chemistry for sequencing motif labeling. The kit is a non-destructive labeing chemistry that will improve the firms ... Bionano Genomics announced today the global launch of its Direct Label and Stain (DLS), ... Bionano Genomics announced today the global launch of its Direct Label and Stain (DLS), a new chemistry for sequencing motif ... labeling. The kit is a non-destructive labeing chemistry that will improve the firms Saphyr genome mapping system. Bionano ...
Stained Label) 2011 from Spain - #15 Wine Spectator Top 100 of 2015 Dark red color with a clean, complex and fresh nose. On the ...
Specific Staining of Red Cell Antigens by the Use of Fluorescein-Labelled Antibody Subject Area: Hematology , Oncology ... B.D. Janković; Specific Staining of Red Cell Antigens by the Use of Fluorescein-Labelled Antibody. Acta Haematol 1 May 1959; 22 ...
Label Options. Labeling Scale. Labeling Time. Features. Mix-n-Stain™ CF® Dye Antibody Labeling Kits. CF® Dyes. ≤5-20 ug IgG. 20 ... Antibody & Protein Labeling Kits. Protein Labeling Kits , Small Ligand Labeling Mix-n-Stain™ Antibody Labeling Kits ... Mix-n-Stain™ Maxi Antibody Labeling Kits, 1 mg Labeling The simplicity and convenience of Mix-n-Stainlabeling, for ... Mix-n-Stainlabeling is covalent, so labeled antibodies can be used for multiplex staining without transfer of dyes/labels ...
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Immunofluorescence; BrdU labeling and imaging. Immunofluorescent staining in adults. Adult male littermate mice were deeply ... Nissl staining and Golgi staining. Nissl staining. For Nissl staining, brains from perfused adult animals were post-fixed in 4 ... Golgi staining and analysis. Golgi-Cox staining was performed according to protocol using the FD Rapid GolgiStain KitTM (FD ... f Nissl-staining of P0 coronal, Cul3+/fl, Cul3+/fl Emx1-Cre, and Cul3fl/fl Emx1-Cre brain sections show severe brain ...
Its important to read the label every time you shop, even though many labels dont offer complete information. ... Line 2 for typical stains or Line 4 for extra tough stains. 2. SOAK 1-6 hours. Depending on severity of stain, some stains may ... Ingredients from the label. What appears on the label: sodium percarbonate, sodium carbonate ... 5) Treat stains while fresh. Always rinse well with cool water. Do not place fabric that has not been thoroughly rinse into ...
These are used as probes, stains, markers, NIR-labels, pH-sensors, ion indicators, chelators and in other applications by life- ... stains and labels. Chemodex is an expert in the synthesis of fluorescent substances derived from fluorophores such as coumarin ... Dil Stain. CDX-D0230. Lipophilic orange-red fluorescent membrane stain that diffuses laterally to stain the entire cell. The ... Stains/Labels/Dyes. - Markers & Probes. - Metabolites. - Building Blocks & Small Molecules. Chemodex is an expert in the ...
Lost your password? Please enter your email address. You will receive mail with link to set new password.. ...
Histochemistry : an explanatory outline of histochemistry and biophysical staining / Richard W. Horobin. by Horobin, Richard W. ...
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In this context, we have developed a fast, label-free 3-D imaging method of the ENS, based on intrinsic signals of the tissue. ... Validation by immunofluorescence and spectral recordings complete our label-free characterization. Finally, we demonstrate the ... Staining, immunofluorescent labeling, and clearing. Due to the long incubation times in each protocol, all reagents used ... Slides were deparaffinized and stained for HES (Hematoxylin/Erythrosine/Saffron): in this protocol, nuclei are stained with ...
His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD27 Ligand Protein, His ... APC-Labeled Human CD27 Ligand Protein, His Tag (CDL-HA248) is produced via conjugation of APC to Human CD27 Ligand Protein, ... Aneuro ActiveMax CellInsight ClinMax ComboX FLAG GENPower GENIUS resDetect Star Ribbon Star Staining ViruStop ... 5e5 of anti-CD27 Ligand CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of ...
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Also, there are stains like white paint all over. There is a mark where the rank insignia and name tape have been removed. ... There are also stains on the cuffs. The zipper slider comes with a cotton strap, but it is missing from this unit. This is a ... Also, there are stains like white paint all over. There is a mark where the rank insignia and name tape have been removed. ...
Previous results from two proficiency panels of intracellular cytokine staining (ICS) from the Cancer Immunotherapy Consortium ... Staining and Labeling Substances * Cytokines Grants and funding * P30 CA016056/CA/NCI NIH HHS/United States ... Previous results from two proficiency panels of intracellular cytokine staining (ICS) from the Cancer Immunotherapy Consortium ... A harmonized approach to intracellular cytokine staining gating: Results from an international multiconsortia proficiency panel ...
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Wheat germ agglutinin and peanut agglutinin displayed heterogeneous staining patterns, binding most avidly to th … ... UEA1 strongly stained 97.2% (106/109) of M-cells, 0.6% (3/516) enterocytes, and 0% (0/28) goblet cells, whereas WBA stained 100 ... When confocal laser scanning images of tissues stained with fluorescein isothiocyanate (FITC)-conjugated UEA1 or WBA were ... Wheat germ agglutinin and peanut agglutinin displayed heterogeneous staining patterns, binding most avidly to the intestine ...
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... the chemical name may appear on the label.) ... OBSERVE WARNINGS ON THE LABEL.. *Swab the stained area with the ... Stains of a rust color on concrete are usually caused by rusting of steel in or on concrete, from use of curing water that ... For Light Shallow Stains: *Oxalic Acid (COOH)2 or (H2C2O4): *A poisonous strong acid that occurs in various plants as oxalates ... For Deep Penetrating Stains: *Dissolve 11 ounces by weight of sodium citrate in 2 quarts of lukewarm water and add 2 quarts and ...
Stained glass with label moulds over. Good belfry above western parapet gable. Federation Gothic style hall c. 1900s, ... The stained glass windows are some of the earliest to be made in the colony, the two in the first bay of the south wall of the ...
Staining and Labeling • Visual Cortex • Visual Pathways • Visual Perception. Duke University * Arts & Sciences * Reload * Login ...
  • Fluorescent nucleic acid stain useful for staining DNA, chromosomes, nuclei and mitochondria. (adipogen.com)
  • The reagents are used as probes, stains, markers, NIR-labels, pH-sensors, ion indicators, chelators and in other applications (e.g. analytical biochemistry, immunoassays and microscopy) by life science researchers in academia, biotechnology and the diagnostics & pharmaceutical industry. (adipogen.com)
  • One of the most popular green fluorescent reagents used for click chemistry labeling of peptides, proteins, nucleotides or even small molecules. (adipogen.com)
  • There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. (genomicglossaries.com)
  • Positive and negative controls must be used to monitor the effectiveness of test procedures that use special stains or reagents, tests with endpoints such as agglutination, color change, or other non-numeric results. (who.int)
  • Reagents should be stored according to manufacturer's instructions, labelled with the date they are opened and put into use, and discarded at the expiration date (see Purchasing and Inventory Module 4). (who.int)
  • Fluorescent label for amine modification and protein conjugation. (adipogen.com)
  • It has a strong blue fluorescence and can be used to create blue fluorescent bioconjugates or labeling synthetic peptides for high-throughput detection. (adipogen.com)
  • Lipophilic orange-red fluorescent membrane stain that diffuses laterally to stain the entire cell. (adipogen.com)
  • AnaSpec offers twelve nucleic acid labeling and fluorescent nucleotides. (anaspec.com)
  • MDI-GSH induced MN had a significant increase in the number of anti-kineticore antibody labeled MN. (cdc.gov)
  • Monomeric Cyanine Nucleic Acid stains allow ultrasensitive fluorescence detection of double-stranded nucleic acids. (thermofisher.com)
  • They are ideally suited for nuclear staining in flow cytometry and fluorescence microscopy applications in fixed cells, along with nucleic acid detection on solid supports and in electrophoresis applications. (thermofisher.com)
  • or on slicing, often combined with hematoxylin and eosin (H and E) staining and followed by imaging of selected single thin sections with bright-field microscopy. (frontiersin.org)
  • Hematoxylin and eosin staining. (cdc.gov)
  • The simplicity and convenience of Mix-n-Stain™ labeling, for conjugation of 1 mg IgG with our CF® Dyes or other dyes. (biotium.com)
  • Combined fast clearing and two-photon volume autofluorescence imaging allow for label-free anatomical studies of intestinal tissue and biopsies at unprecedented speed and detail. (frontiersin.org)
  • They provide extreme protection by blocking out the sun's harmful UV rays, as well as resisting stains caused by mold & mildew. (defywoodstain.com)
  • For best results, use DEFY Wood Cleaner to remove dirt, mill glaze, mold or mildew stains, graying and other foreign matter that might block surface pores and interfere with product penetration. (defywoodstain.com)
  • Whether it is for protein electrophoresis or western blot, our pre-stained protein markers help you quickly determine the molecular weight of the target protein or evaluate the transfer efficiency. (acrobiosystems.com)
  • Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. (biotium.com)
  • Widely used cationic bluish-green dyes for both light and electron microscopy to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. (adipogen.com)
  • Apply a mold stain remover, such as household soap, white vinegar, or bleach, and use a toothbrush to brush off the surface mold. (puroclean.com)
  • To reduce exposure to toluene, you should use products that contain it (such as paints, nail polish, glues, inks, and stain removers) in well-ventilated areas. (cdc.gov)
  • Results of search for 'su:{Staining and labeling. (who.int)
  • Baldwin's bill would mandate prominent labeling of country-of-origin and seller location for all products sold on the internet. (yahoo.com)
  • PS: Be sure to clean your glass well with glass cleaner before transferring the faux stained glass black outlines! (remodelaholic.com)
  • The same fluid, water reactive formula crafters have come to love in the Distress Stains available in a spray. (stampersanonymous.com)
  • Mold stains can develop on clothes from exposure to water or improper storage. (puroclean.com)
  • Since mold stains are hard to remove, you should also pre-soak your stained garment before washing it. (puroclean.com)
  • White vinegar is suitable for disinfecting and cleaning stubborn mold stains like white mold or green mold . (puroclean.com)
  • Repeat the process if you've followed the above steps and the mold stains persist. (puroclean.com)
  • Accumulation of β-amyloid precursor protein was observed in white matter tracts in both models in areas with preserved and reduced myelin staining. (lu.se)
  • In contrast, the glial scar, evaluated by glial fibrillary acidic protein staining, showed its highest intensity 21 days post-injury in both models. (lu.se)
  • Jane Iredale Just Kissed Lip & Cheek Stain in Forever Pink is a multipurpose stain that uses all natural ingredients to enhance your natural colouring. (naturisimo.com)
  • Always store household chemicals in their original labeled containers. (cdc.gov)
  • The article Skeletal Muscle - Structure and Histology provides a review of normal skeletal muscle histology and ultrastructure, including the histologic appearance of normal muscle with some of the various stains that are used for the processing of muscle biopsies. (medscape.com)
  • The stained glass windows are some of the earliest to be made in the colony, the two in the first bay of the south wall of the nave being the earliest windows to be commissioned in New South Wales. (wikipedia.org)
  • Toluene can be found in gasoline products, paints, stain removers, Toluene may affect the nervous system. (cdc.gov)
  • Previous results from two proficiency panels of intracellular cytokine staining (ICS) from the Cancer Immunotherapy Consortium and panels from the National Institute of Allergy and Infectious Disease and the Association for Cancer Immunotherapy highlight the variability across laboratories in reported % CD8+ or % CD4+ cytokine-positive cells. (nih.gov)
  • Can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. (adipogen.com)
  • If you'd like a more colorful stained glass window, use the colored Gallery Glass paints instead like Madigan added to her sidelights here . (remodelaholic.com)
  • DEFY Solid Color Wood Stain is a durable acrylic, opaque wood finish that protects against the damaging effects of the elements, on all wood surfaces. (defywoodstain.com)
  • DEFY Solid Color Wood Stain can be used all wood types including pressure treated lumber, pine, cedar, redwood, mahogany, Ipe and all other natural wood surfaces. (defywoodstain.com)
  • All wood surfaces must be clean, porous, and visibly dry before applying DEFY Solid Color Wood Stain. (defywoodstain.com)
  • While surface is still wet from cleaning or stripping, use DEFY Wood Brightener to neutralize the wood, restore the wood to its natural color, and open the pores of the wood, allowing the stain to be more easily absorbed. (defywoodstain.com)
  • Alternatively, dye your garment a darker color - this may cover the stain for good. (puroclean.com)
  • Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. (biotium.com)
  • Submerge stained items in solution after dissolved. (ewg.org)
  • They've sent a letter to Congress urging support for country-of-origin labeling. (yahoo.com)
  • Both the House and Senate should support country-of-origin labeling for all e-commerce transactions. (yahoo.com)
  • A lip and cheek stain that provides long-lasting, custom colour to lips and cheeks. (naturisimo.com)
  • Apply Jane Iredale Just Kissed Lip & Cheek Stain to lips and cheeks for buildable, custom colour. (naturisimo.com)
  • Requiring country-of-origin labeling should be a no-brainer, since current U.S. law only mandates that a product's external packaging display its place of origin. (yahoo.com)
  • At present, however, e-commerce websites often don't include country-of-origin information - since labeling laws were written before the advent of online shopping. (yahoo.com)
  • Whether it's a 6, 7 or 8-string, every Iron Label axe is matched perfectly with American-made EMG® or DiMarzio® pickups. (guitar-compare.com)
  • My window design is all straight lines, which made it easy to draw and easy to create using primarily the peel and stick DIY stained glass leading lines. (remodelaholic.com)
  • Wheat germ agglutinin and peanut agglutinin displayed heterogeneous staining patterns, binding most avidly to the intestine goblet cells. (nih.gov)
  • When confocal laser scanning images of tissues stained with fluorescein isothiocyanate (FITC)-conjugated UEA1 or WBA were compared with the appearance of the same tissues under the scanning electron microscope (SEM), UEA1 strongly stained 97.2% (106/109) of M-cells, 0.6% (3/516) enterocytes, and 0% (0/28) goblet cells, whereas WBA stained 100% (83/83) M-cells, 1.7% (6/361) enterocytes, and 5.3% (1/19) goblet cells. (nih.gov)
  • Micronuclei kinetichore from cytokinesis-blocked cells were labeled by immunofluorescent staining. (cdc.gov)
  • Cells were washed, methanol fixed, treated with Tween 20 buffer, then labeled with antikineticore and fluoresceinated goat anti-human IgG antibodies to discern the presence of centromere within the micronuclei. (cdc.gov)
  • 2) Follow manufacture's instructions for water temperature and for cleaning garment or item 3) Do not use on wool, wool blends, silk, silk blends, leather or on fabrics labeled dry clean only. (ewg.org)
  • Depending on severity of stain, some stains may require time to soak. (ewg.org)
  • On the cheap, of course, with a faux stained glass window treatment … yes you can stain glass in an existing window! (remodelaholic.com)
  • It's a beautiful classic design and adds privacy to a plain window, and it's easy to make your own stained glass at home! (remodelaholic.com)
  • Grab this inexpensive privacy film for a one-step faux stained glass window. (remodelaholic.com)
  • If this occurs, the GelGreen will no longer work for gel staining. (biotium.com)
  • 6) Some stains require repeated or longer treatments. (ewg.org)
  • eClassical.com is a completely virtual record label and a secure online store open 7 days a week, 365 days a year. (eclassical.com)
  • Used for labeling of cell-surface functional groups (glycophorins). (adipogen.com)
  • So make sure to spot-test first and read the label. (puroclean.com)
  • With Luxol Fast Blue and RIP staining, myelin loss was observed in both models, in all evaluated regions and at all post-injury. (lu.se)
  • Bionano Genomics announced today the global launch of its Direct Label and Stain (DLS), a new chemistry for sequencing motif labeling. (genomeweb.com)
  • Former prime minister Gordon Brown , for whom the term "statesman" is entirely appropriate, has described the global vaccination rollout as a "stain on our soul" . (yahoo.com)