The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)
Computer-assisted processing of electric, ultrasonic, or electronic signals to interpret function and activity.
The visualization of deep structures of the body by recording the reflections or echoes of ultrasonic pulses directed into the tissues. Use of ultrasound for imaging or diagnostic purposes employs frequencies ranging from 1.6 to 10 megahertz.
A subfield of acoustics dealing in the radio frequency range higher than acoustic SOUND waves (approximately above 20 kilohertz). Ultrasonic radiation is used therapeutically (DIATHERMY and ULTRASONIC THERAPY) to generate HEAT and to selectively destroy tissues. It is also used in diagnostics, for example, ULTRASONOGRAPHY; ECHOENCEPHALOGRAPHY; and ECHOCARDIOGRAPHY, to visually display echoes received from irradiated tissues.
Recording of electric currents developed in the brain by means of electrodes applied to the scalp, to the surface of the brain, or placed within the substance of the brain.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
The number of times the HEART VENTRICLES contract per unit of time, usually per minute.
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
Severe distortions in the development of many basic psychological functions that are not normal for any stage in development. These distortions are manifested in sustained social impairment, speech abnormalities, and peculiar motor movements.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
A continuing periodic change in displacement with respect to a fixed reference. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.
An umbrella term used to describe a pattern of disabilities and abnormalities that result from fetal exposure to ETHANOL during pregnancy. It encompasses a phenotypic range that can vary greatly between individuals, but reliably includes one or more of the following: characteristic facial dysmorphism, FETAL GROWTH RETARDATION, central nervous system abnormalities, cognitive and/or behavioral dysfunction, BIRTH DEFECTS. The level of maternal alcohol consumption does not necessarily correlate directly with disease severity.
Condensation products of aromatic amines and aldehydes forming azomethines substituted on the N atom, containing the general formula R-N:CHR. (From Grant & Hackh's Chemical Dictionary, 5th ed)
A disorder beginning in childhood. It is marked by the presence of markedly abnormal or impaired development in social interaction and communication and a markedly restricted repertoire of activity and interest. Manifestations of the disorder vary greatly depending on the developmental level and chronological age of the individual. (DSM-V)
A conjugated protein which is the oxygen-transporting pigment of muscle. It is made up of one globin polypeptide chain and one heme group.
Carbon monoxide (CO). A poisonous colorless, odorless, tasteless gas. It combines with hemoglobin to form carboxyhemoglobin, which has no oxygen carrying capacity. The resultant oxygen deprivation causes headache, dizziness, decreased pulse and respiratory rates, unconsciousness, and death. (From Merck Index, 11th ed)
Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Thin strands of transparent material, usually glass, that are used for transmitting light waves over long distances.
A quantitative prediction of the biological, ecotoxicological or pharmaceutical activity of a molecule. It is based upon structure and activity information gathered from a series of similar compounds.
Hydrocarbons with more than one triple bond; or an oxidized form of POLYENES. They can react with SULFUR to form THIOPHENES.
Electromagnetic waves with frequencies between about 3 kilohertz (very low frequency - VLF) and 300,000 megahertz (extremely high frequency - EHF). They are used in television and radio broadcasting, land and satellite communications systems, radionavigation, radiolocation, and DIATHERMY. The highest frequency radio waves are MICROWAVES.
The application of electronic, computerized control systems to mechanical devices designed to perform human functions. Formerly restricted to industry, but nowadays applied to artificial organs controlled by bionic (bioelectronic) devices, like automated insulin pumps and other prostheses.
Surgical procedures conducted with the aid of computers. This is most frequently used in orthopedic and laparoscopic surgery for implant placement and instrument guidance. Image-guided surgery interactively combines prior CT scans or MRI images with real-time video.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
Methods of creating machines and devices.
The fourth planet in order from the sun. Its two natural satellites are Deimos and Phobos. It is one of the four inner or terrestrial planets of the solar system.
Conducting a biopsy procedure with the aid of a MEDICAL IMAGING modality.
Discrete concentrations of energy, apparently massless elementary particles, that move at the speed of light. They are the unit or quantum of electromagnetic radiation. Photons are emitted when electrons move from one energy state to another. (From Hawley's Condensed Chemical Dictionary, 11th ed)
A carotenoid constituent of visual pigments. It is the oxidized form of retinol which functions as the active component of the visual cycle. It is bound to the protein opsin forming the complex rhodopsin. When stimulated by visible light, the retinal component of the rhodopsin complex undergoes isomerization at the 11-position of the double bond to the cis-form; this is reversed in "dark" reactions to return to the native trans-configuration.
Using an INTERNET based personal journal which may consist of reflections, comments, and often hyperlinks.
The field of information science concerned with the analysis and dissemination of medical data through the application of computers to various aspects of health care and medicine.
The application of technology to the solution of medical problems.
The application of scientific knowledge to practical purposes in any field. It includes methods, techniques, and instrumentation.
Beliefs and practices concerned with producing desired results through supernatural forces or agents as with the manipulation of fetishes or rituals.
Abnormally rapid heartbeats with sudden onset and cessation.
Automated systems applied to the patient care process including diagnosis, therapy, and systems of communicating medical data within the health care setting.

Chlamydomonas chloroplast ferrous hemoglobin. Heme pocket structure and reactions with ligands. (1/2113)

We report the optical and resonance Raman spectral characterization of ferrous recombinant Chlamydomonas LI637 hemoglobin. We show that it is present in three pH-dependent equilibrium forms including a 4-coordinate species at acid pH, a 5-coordinate high spin species at neutral pH, and a 6-coordinate low spin species at alkaline pH. The proximal ligand to the heme is the imidazole group of a histidine. Kinetics of the reactions with ligands were determined by stopped-flow spectroscopy. At alkaline pH, combination with oxygen, nitric oxide, and carbon monoxide displays a kinetic behavior that is interpreted as being rate-limited by conversion of the 6-coordinate form to a reactive 5-coordinate form. At neutral pH, combination rates of the 5-coordinate form with oxygen and carbon monoxide were much faster (>10(7) microM-1 s-1). The dissociation rate constant measured for oxygen is among the slowest known, 0.014 s-1, and is independent of pH. Replacement of the tyrosine 63 (B10) by leucine or of the putative distal glutamine by glycine increases the dissociation rate constant 70- and 30-fold and increases the rate of autoxidation 20- and 90-fold, respectively. These results are consistent with at least two hydrogen bonds stabilizing the bound oxygen molecule, one from tyrosine B10 and the other from the distal glutamine. In addition, the high frequency (232 cm-1) of the iron-histidine bond suggests a structure that lacks any proximal strain thus contributing to high ligand affinity.  (+info)

EPR characterization of axial bond in metal center of native and cobalt-substituted guanylate cyclase. (2/2113)

The nature of the metal-proximal base bond of soluble guanylate cyclase from bovine lung was examined by EPR spectroscopy. When the ferrous enzyme was mixed with NO, a new species was transiently produced and rapidly converted to a five-coordinate ferrous NO complex. The new species exhibited the EPR signal of six-coordinate ferrous NO complex with a feature of histidine-ligated heme. The histidine ligation was further examined by using the cobalt protoporphyrin IX-substituted enzyme. The Co2+-substituted enzyme exhibited EPR signals of a broad g perpendicular;1 component and a g;1 component with a poorly resolved triplet of 14N superhyperfine splittings, which was indicative of the histidine ligation. These EPR features were analogous to those of alpha-subunits of Co2+-hemoglobin in tense state, showing a tension on the iron-histidine bond of the enzyme. The binding of NO to the Co2+-enzyme markedly stimulated the cGMP production by forming the five-coordinate NO complex. We found that N3- elicited the activation of the ferric enzyme by yielding five-coordinate high spin N3- heme. These results indicated that the activation of the enzymes was initiated by NO binding to the metals and proceeded via breaking of the metal-histidine bonds, and suggested that the iron-histidine bond in the ferric enzyme heme was broken by N3- binding.  (+info)

Q-Band resonance Raman investigation of turnip cytochrome f and Rhodobacter capsulatus cytochrome c1. (3/2113)

The results of a comprehensive Q-band resonance Raman investigation of cytochrome c1 and cytochrome f subunits of bc1 and b6f complexes are presented. Q-band excitation provides a particularly effective probe of the local heme environments of these species. The effects of protein conformation (particularly axial ligation) on heme structure and function were further investigated by comparison of spectra obtained from native subunits to those of a site directed c1 mutant (M183L) and various pH-dependent species of horse heart cytochrome c. In general, all species examined displayed variability in their axial amino acid ligation that suggests a good deal of flexibility in their hemepocket conformations. Surprisingly, the large scale protein rearrangements that accompany axial ligand replacement have little or no effect on macrocycle geometry in these species. This indicates the identity and/or conformation of the peptide linkage between the two cysteines that are covalently linked to the heme periphery may determine heme geometry.  (+info)

Altered ligand rebinding kinetics due to distal-side effects in hemoglobin chico (Lysbeta66(E10) --> thr). (4/2113)

Hb Chico is an unusual human hemoglobin variant that has lowered oxygen affinity, but unaltered cooperativity and anion sensitivity. Previous studies showed these features to be associated with distal-side heme pocket alterations that confer increased structural rigidity on the molecule and that increase water content in the beta-chain heme pocket. We report here that the extent of nanosecond geminate rebinding of oxygen to the variant and its isolated beta-chains is appreciably decreased. Structural alterations in this variant decrease its oxygen recombination rates without significantly altering rates of migration out of the heme pocket. Data analysis indicates that one or more barriers that impede rebinding of oxygen from docking sites in the heme pocket are increased, with less consequence for CO rebinding. Resonance Raman spectra show no significant alterations in spectral regions sensitive to interactions between the heme iron and the proximal histidine residue, confirming that the functional differences in the variant are due to distal-side heme pocket alterations. These effects are discussed in the context of a schematic representation of heme pocket wells and barriers that could aid the design of novel hemoglobins with altered ligand affinity without loss of the normal allosteric responses that facilitate unloading of oxygen to respiring tissues.  (+info)

Absence in amphotericin B-spiked human plasma of the free monomeric drug, as detected by SERS. (5/2113)

Using surface enhanced Raman spectroscopy (SERS) which enables us to specifically detect traces of monomeric amphotericin B (AmB), we were able to show that in a 10(-5) M AmB suspension, the concentration of free drug was below 10(-8) M in the presence of low density lipoproteins (LDL) or plasma. The affinity constant of AmB for LDL determined from electronic absorption data, was found to be 4 x 10(6) M(-1). Therefore, since AmB appears to be in the majority bound to lipoproteins under in vivo conditions, its toxicity should not result from the induction of host-cell transmembrane permeability but rather from the internalization of the AmB-LDL complex.  (+info)

Purification, redox and spectroscopic properties of the tetraheme cytochrome c isolated from Rubrivivax gelatinosus. (6/2113)

The tetraheme cytochrome c subunit of the Rubrivivax gelatinosus reaction center was isolated in the presence of octyl beta-D-thioglucoside by ammonium sulfate precipitation and solubilization at pH 9 in a solution of Deriphat 160. Several biochemical properties of this purified cytochrome were characterized. In particular, it forms small oligomers and its N-terminal amino acid is blocked. In the presence or absence of diaminodurene, ascorbate and dithionite, different oxidation/reduction states of the isolated cytochrome were studied by absorption, EPR and resonance Raman spectroscopies. All the data show two hemes quickly reduced by ascorbate, one heme slowly reduced by ascorbate and one heme only reduced by dithionite. The quickly ascorbate-reduced hemes have paramagnetic properties very similar to those of the two low-potential hemes of the reaction center-bound cytochrome (gz = 3.34), but their alpha band is split with two components peaking at 552 nm and 554 nm in the reduced state. Their axial ligands did not change, being His/Met and His/His, as indicated by the resonance Raman spectra. The slowly ascorbate-reduced heme and the dithionite-reduced heme are assigned to the two high-potential hemes of the bound cytochrome. Their alpha band was blue-shifted at 551 nm and the gz values decreased to 2.96, although the axial ligations (His/Met) were conserved. It was concluded that the estimated 300 mV potential drop of these hemes reflected changes in their solvent accessibility, while the reduction in gz indicates an increased symmetry of their cooordination spheres. These structural modifications impaired the cytochrome's essential function as the electron donor to the photooxidized bacteriochlorophyll dimer of the reaction center. In contrast to its native state, the isolated cytochrome was unable to reduce efficiently the reaction center purified from a Rubrivivax gelatinosus mutant in which the tetraheme was absent. Despite the conformational changes of the cytochrome, its four hemes are still divided into two groups with a pair of low-potential hemes and a pair of high-potential hemes.  (+info)

An anomaly in the resonance Raman spectra of cytochrome P-450cam in the ferrous high-spin state. (7/2113)

Resonance Raman spectra of cytochrome P-450cam (P-450cam) and its enzymatically inactive form (P-420) in various oxidation and spin states were measured for the first time. The Raman spectrum of reduced P-450cam was unusual in the sense that the "oxidation-state marker" appeared at an unexpectedly lower frequency (1346 cm-1) in comparison with those of other reduced hemoproteins (approximately 1355-approximately 1365 cm-1), whereas that of oxidized P-450cam was located at a normal frequency. This anomaly in the Raman spectrum of reduced P-450cam can be explained by assuming electron delocalization from the fifth ligand, presumably a thiolate anion, to the antibonding pi orbital of the porphyrin ring. The corresponding Raman line of reduced P-420 appeared at a normal frequency (1360 cm-1), suggesting a status change or replacement of the fifth ligand upon conversion from P-450cam to P-420. The Raman spectrum of reduced P-450cam-metyrapone complex was very similar to that of ferrous cytochrome b5.  (+info)

Identification by UV resonance Raman spectroscopy of an imino tautomer of 5-hydroxy-2'-deoxycytidine, a powerful base analog transition mutagen with a much higher unfavored tautomer frequency than that of the natural residue 2'-deoxycytidine. (8/2113)

UV resonance Raman spectroscopy was used to detect and estimate the frequency of the unfavored imino tautomer of the transition mutagen 5-hydroxy-2'-deoxycytidine (HO5dCyt) in its anionic form. In DNA, this 2'-deoxycytidine analog arises from the oxidation of 2'-deoxycytidine and induces C --> T transitions with 10(2) greater frequency than such spontaneous transitions. An imino tautomer marker carbonyl band (approximately 1650 cm-1) is enhanced at approximately 65 degrees C against an otherwise stable spectrum of bands associated with the favored amino tautomer. This band is similarly present in the UV resonance Raman spectra of the imino cytidine analogs N3-methylcytidine at high pH and N4-methoxy-2'-deoxycytidine at pH 7 and displays features attributable to the imino form of C residues and their derivatives. The fact that the imino tautomer of HO5dCyt occurs at a frequency consistent with its high mutagenic enhancement lends strong support to the hypothesis that unfavored base tautomers play important roles in the mispair intermediates of replication leading to substitution mutations.  (+info)

We demonstrate ordered orientation of the hydration water at the surface of phospholipid bilayers by use of coherent anti-Stokes Raman scattering (CARS) microscopy, a highly sensitive vibrational imaging method recently developed. We investigated negatively charged POPS (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine) and neutral POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) multilamellar onions dispersed in deuterated dodecane. The imaging contrast based on the CARS signal from the H2O stretching vibration shows a clear dependence on the excitation field polarization. Our results provide direct experimental evidence that water molecules close to the phospholipid bilayer surface are ordered with the symmetry axis along the direction normal to the bilayer. Moreover, the amount of ordered water molecules depends on the lipid polar group. The spectral profile for the interlamellar water shows that the water molecules bound to the bilayer surface are less hydrogen-bonded and exhibit a ...
We report a mechanistic analysis of photodamage in coherent anti-Stokes Raman scattering (CARS) microscopy. Photodamage to the myelin sheath in spinal tissues is induced by using the point scan mode and is featured by myelin splitting and shockwaves with broadband emission. Our measurement of photodamage rate versus the excitation power reveals that both linear and nonlinear mechanisms are involved. Moreover, we show that vibrational absorption induced by coherent Raman processes significantly contributes to the nonlinear damage at high peak powers. For CARS imaging of cultured cells, the photodamage is characterized by plasma membrane blebbing and is dominated by a second order mechanism. Our study suggests that for dense samples such as the myelin sheath, CARS imaging induced photodamage can be minimized by using laser beams with relatively long near IR wavelengths and a repetition rate of a few MHz. For less dense samples such as cultured cells, laser pulses of higher repetition rates are ...
Coherent Anti-Stokes Raman Scattering (CARS)-microscopy has in recent years developed as a promising microscopical technique for label-free microscopy of living cells. We propose a new concept, spectral focusing, for highly efficient coherent anti-Stokes Raman scattering (CARS) microscopy. It allows optimal use of the excitation energy of femtosecond laser pulses in terms of generated CARS signal against a low background. This is accomplished by introducing a linear chirp in the excitation pulses. The temporal delaying of the excitation pulses can be used to record vibrational spectra of a sample. Despite the inherently broad spectral width of the excitation pulses, the technique enables resolution of spectral features 60 times narrower than the bandwidth of the probe light. First applications of this technique are exemplified with CARS of micron sized crystallites of sodium nitroprusside, a commonly used hypotensive agent ...
Nanoparticulate drug delivery is known to greatly improve the efficacy of pharmaceuticals and has found a wide range of applications with different administration methods including oral, intravenous, transcutaneous and ocular routes. However, the mechanisms by which these nanoparticles travel through, interact with, and modify tissues and how this relates to the improved drug performance are still unclear. These are critical questions that need to be answered to optimise the properties of future pharmaceuticals, dose rates and frequencies, and reduce potential side effects. Our ability to answer these questions is greatly hindered by the fact that there is currently no imaging modality available to directly visualise such small particles and the structure and function of the surrounding tissue, without the aid of contrast agents or highly invasive methods. Current imaging modalities derive image contrast of the nanoparticles and/or the surrounding tissues by means of external labels. We present ...
The rather thin outermost layer of the mammalian skin, stratum corneum (SC), is a complex biomembrane which separates the water rich inside of the body from the dry outside. The skin surface can be exposed to rather extreme variations in ambient conditions (e.g. water activity, temperature and pH), with potential effects on the barrier function. Increased understanding of how the barrier is affected by such changes is highly relevant for regulation of transdermal uptake of exogenous chemicals. In the present study we investigate the effect of hydration and the use of a well-known humectant, urea, on skin barrier ultrastructure by means of confocal Raman microspectroscopy. We also perform dynamic vapor sorption (DVS) microbalance measurements to examine the water uptake capacity of SC pretreated with urea. Based on novel Raman images, constructed from 20 spectral maps, we can distinguish large water inclusions within the skin membrane exceeding the size of fully hydrated corneocytes. We show that ...
Raman spectroscopy has numerous applications in the field of biology. One such application is the simultaneously measurement of the concentration of multiple biochemical components in low volume aqueous mixtures, for example, a single drop of blood serum. Over twenty years ago, it was shown for the first time that it was possible to estimate the concentration of glucose, urea, and lactic acid in mixture by combining Raman Spectroscopy with Partial Least Squares Regression analysis. This was followed by numerous contributions in the literature designed to increase the number of components and reduce the limits of concentration that could be simultaneously measured using Raman spectroscopy, by developing various optical architectures to maximise the signal to noise ratio. The aim of this paper is to demonstrate the potential of a confocal Raman microscopy system for multicomponent analysis for the case of physiologically relevant mixtures of glucose, urea, and lactic acid.. ...
Confocal Raman microscopy can identify particles in the 5-50 ?m range and can bridge the gap between micro-FT-IR and SEM-EDS analyses.
TY - JOUR. T1 - RESONANCE RAMAN SCATTERING STUDIES OF THE QUATERNARY STRUCTURE TRANSITION IN HEMOGLOBIN.. AU - Rousseau, D. L.. AU - Ondrias, M. R.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - The oxygen-binding energy difference, the free energy of cooperativity, is a thermodynamic property, and its location can be determined from an examination of the deoxygenated and liganded proteins in both their high affinity and low affinity structures. An assessment of the energetic differences between these four steady-state protein structures should elucidate which interactions contain the free energy of cooperativity. Resonance Raman scattering plays a very important role in determining these interactions, since it is a very sensitive probe of the bonds involving the heme group. By examining the resonance Raman spectrum of the heme in both quaternary structures in the liganded and in the deoxy forms, it may in principle be determined if the free energy of cooperativity is localized in any heme-protein ...
TY - JOUR. T1 - Stimulated Raman scattering microscopy with long wavelengths for improved imaging depth. AU - Moester, Miriam J.B.. AU - Zada, Liron. AU - Fokker, Bart. AU - Ariese, Freek. AU - de Boer, Johannes F.. PY - 2019/9. Y1 - 2019/9. N2 - Stimulated Raman scattering (SRS) imaging is a fast, label-free, and sensitive technique to map the distribution of a vibrational species in a microscopy setting. It has great potential for applications in many fields, such as lipid imaging in biomedicine. However, depth penetration of the light into the sample is an issue with any light-based technique, especially with multiphoton techniques such as SRS. Using longer wavelengths allows deeper penetration into densely scattering materials, but applying wavelengths above 1,500 nm is challenging technically. We have built a flexible SRS microscope system capable of imaging with a combination of 1,064 nm and wavelengths over 1,500 nm, using the idler output of an optical parametric oscillator (OPO). For ...
TY - JOUR. T1 - Ultraviolet resonance Raman study of proline isomerization. AU - Harhay, Gregory P.. AU - Hudson, Bruce S.. PY - 1991/1/1. Y1 - 1991/1/1. N2 - The ultraviolet resonance Raman spectra of aqueous solutions of proline dipeptides obtained with 200- and 218-nm excitation at pH 1.5 and 6.5 are reported. The cis/trans isomer ratio for these dipeptides is known to depend on pH. The amide II′ like mode of the X-Pro peptide (imide) linkage at 1487 cm-1, involving predominantly the motions of the peptide carbon and nitrogen, is the most strongly enhanced band in the 200-250-nm region. We observe that this band does not shift appreciably with the change in isomer ratio associated with the change in pH. The same behavior is observed with the 1467-cm-1 band of aqueous polyproline, which has undergone a salt-induced isomerization. We conclude from these observations that contrary to earlier evidence the position of the amide II′ like band is not diagnostic for the isomeric state of the ...
TY - JOUR. T1 - Resonance Raman and surface-enhanced resonance Raman studies of polymer-modified electrodes which mimic heme enzymes. AU - Bell, Steven. AU - Devenney, M.D.. AU - Grimshaw, J.. AU - Hara, S.. AU - Rice, J.H.. AU - Trocha-Grimshaw, J.. PY - 1998. Y1 - 1998. N2 - Iron-5,10,15,20-tetraphenylporphyrin (FeTPP) has been incorporated into films of a coordinating hydrogel polymer support medium, poly(gamma-ethyl-L-glutamate) (PEG) functionalised with imidazole pendant arms (PEG-Im), and studied in situ on silver electrodes using a combination of both resonance Raman (RR) and surface-enhanced resonance Raman (SERR) spectroscopy. The SERR spectra give information on the portion of the film close to the electrode surface while RR spectra probe the. AB - Iron-5,10,15,20-tetraphenylporphyrin (FeTPP) has been incorporated into films of a coordinating hydrogel polymer support medium, poly(gamma-ethyl-L-glutamate) (PEG) functionalised with imidazole pendant arms (PEG-Im), and studied in situ on ...
The clinical development of therapeutic peptides has been restricted to peptides for non-CNS diseases and parenteral dosage forms due to the poor permeation of peptides across the gastrointestinal mucosa and the blood-brain barrier. Quaternary ammonium palmitoyl glycol chitosan (GCPQ) nanoparticles facilitate the brain delivery of orally administered peptides such as leucine(5)-enkephalin, and here we examine the mechanism of GCPQ facilitated oral peptide absorption and brain delivery. By analyzing the oral biodistribution of radiolabeled GCPQ nanoparticles, the oral biodistribution of the model peptide leucine(5)-enkephalin and coherent anti-Stokes Raman scattering microscopy tissue images after an oral dose of deuterated GCPQ nanoparticles, we have established a number of facts. Although 85-90% of orally administered GCPQ nanoparticles are not absorbed from the gastrointestinal tract, a peak level of 2-3% of the oral GCPQ dose is detected in the blood 30 min after dosing, and these GCPQ ...
1. Wang P, Liu B, Zhang DL, Belew, MY, Tissenbaum HA, Cheng JX*. Imaging Lipid Metabolism in Live Caenorhabditis elegans Using Fingerprint Vibrations. Angewandte Chemie International Edition. 2014; 53:11787-11792 (In press; Impact factor: 13.7). 2. Wang P, Li JJ, Wang P, Hu CR, Zhang DL, Sturek, M, Cheng JX*. Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy. Angewandte Chemie International Edition. 2013; 52:13042-6 (Impact factor: 13.7). 3. Zhang D#, Wang P#, Slipchenko MN, Ben-Amotz D, Weiner AM, Cheng JX*. Quantitative vibrational imaging by hyperspectral stimulated Raman scattering microscopy and multivariate curve resolution analysis. Analytical chemistry. 2013;85(1):98-106. # Equal contribution first author. (Impact factor: 5.8). 4. Liao CS#, Slipchenko MN#, Wang P#, Hu CR, Li JJ, Oglesbee RA, Cheng JX*. Microsecond Scale Vibrational Spectroscopic Imaging by Multiplex Stimulated Raman Scattering Microscopy. Light: ...
The ordinary Raman scattering (ORS) spectra of virion protein of Granulosis Virus of Cabbage Butterfly Preris rapae (PrGV-VP) and their surface-enhanced Raman scattering (SERS) spectra adsorbed on the surface of silver colloid particles have been found that the Raman signal in the SERS spectra was enhanced by a factor of about 8 X 102. In ORS spectra of PrGV-VP, it indicates that PrGV-VP has a predominantly random-coil secondary structures. All tyrosine residues are exposed. And the PrGV-VP has a gauche-gauche- gauche configuration of the C-C-S-S-C-C linkage. In SERS spectra of PrGV-VP, the intensities of bands related to COO group and NH2 group vibrations are relatively strong. We interpret that the PrGV-VP are adsorbed on the Ag surface through both the carboxylate groups and the amino groups. The efficient enhancement of amide I and amide II vibrations are impeded, because the peptide groups of PrGV-VP are screened by the side-chains amino acid residues. The Raman enhancement of PrGV-VP has a ...
TY - JOUR. T1 - Raman difference studies of GDP and GTP binding to c-Harvey ras. AU - Wang, J. H.. AU - Xiao, D. G.. AU - Deng, H.. AU - Webb, Martin R.. AU - Callender, Robert. N1 - Copyright: Copyright 2007 Elsevier B.V., All rights reserved.. PY - 1998/8/4. Y1 - 1998/8/4. N2 - The vibrational spectra of phosphate modes for GDP and GTP bound to the c-Harvey p21(ras) protein have been determined using 18O isotope edited Raman difference spectroscopy. A number of the phosphate stretch frequencies are changed upon GDP/GTP binding to ras, and the results are analyzed by ab initio calculations and through the use of empirical relationships that relate bond orders and bond lengths to vibrational frequencies. Bound GDP is found to be strongly stabilized by its interactions, mostly electrostatic, with the active site Mg2+. Bound GTP also interacts with the active site Mg2+ via its β-phosphate group, as expected on the basis of crystallographic studies of bound GppNp. The angle between the nonbridging ...
There are many examples emphasizing the importance of particle analysis - be it in the API analysis of drugs, adhesives, recycling, electronics manufacturing, biomedical products and much more. Reverse engineering and the investigation of causes of defects in manufacturing processes can also require comprehensive particle analysis. One of the challenges in analyzing microparticles is the size of the particles themselves and the frequent incorporation of these contaminants into matrices. Particle defects can affect product quality, appearance and performance. The SENTERRA II confocal Raman microscope is a powerful tool for the analysis of microparticles without sample preparation and even within sample containers. In our application note, we have summarized some applications to give you an overview of the possibilities of confocal Raman microscopy. ...
This well established symposium will cover various aspects of modern Raman microscopy and detail the advantages of confocal Raman imaging and its applications. Scientific talks from distinguished speakers in academia and industry, the contributed talk and poster sessions, and the instrument demonstration will give the participants a deeper understanding of confocal Raman imaging.
The identification of normal and cancer breast tissue of rats was investigated using high-frequency (HF) FT-Raman spectroscopy with a near-infrared excitation source on in vivo and ex vivo measurements. Significant differences in the Raman intensities of prominent Raman bands of lipids and proteins structures (2,800-3,100 cm(-1)) as well as in the broad band of water (3,100-3,550 cm(-1)) were observed in mean normal and cancer tissue spectra. The multivariate statistical analysis methods of principal components analysis (PCA) and linear discriminant analysis (LDA) were performed on all high-frequency Raman spectra of normal and cancer tissues. LDA results with the leave-one-out cross-validation option yielded a discrimination accuracy of 77.2, 83.3, and 100% for in vivo transcutaneous, in vivo skin-removed, and ex vivo biopsy HF Raman spectra. Despite the lower discrimination value for the in vivo transcutaneous measurements, which could be explained by the breathing movement and skin ...
Confocal Raman microscopy is a high-resolution imaging technique that is widely used for the characterization of materials and specimens in terms of thei...
TY - GEN. T1 - Functionalized bio-nanoparticles in immunosensing using surface-enhanced raman scattering methods. AU - Lin, Chi Chang. AU - Yu, Chen Yuan. AU - Yang, Ying Mei. AU - Chen, Yan Fu. AU - Chang, Hsien Chang. PY - 2009/10/12. Y1 - 2009/10/12. N2 - An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and Surface-Enhanced Raman Spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (DTNB) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-DTNB, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and highthroughput screening of antibodies.. AB - An unique, ...
Dr. Matthias Meier (IMTEK, University of Freiburg) Raman microscopy is a powerful optical technology able to insight into the organic and inorganic chemical composition of living cells. By providing specific information with high spatial resolution about vibrational energy levels of chemical bonds and molecules, Raman microscopy can fingerprint metabolic states of cells. On the other hand microfluidic large-scale integration technology has demonstrate to control precisely the chemical microenvironment of hundreds of cell cultures in parallel with over weeks. In this funded BIOSS project we aim to couple microfluidic chip technology and raman microscopy to study lipid signaling between and in primary human adipocytes. Lipid droplets within adipocytes are the largest energy reservoir in the human body. External hormone, and nutrient availability tightly regulate the build-up (lipogenesis) and break down (lipolysis) of the lipid droplets. Disruption of the regulatory signal network controlling ...
A great deal of effort has been focused on exploring the underlying molecular mechanism of osteoarthritis (OA) especially at the cellular level. We report a confocal Raman spectroscopic investigation on human osteoarthritic chondrocytes. The objective of this investigation is to identify molecular features and the stage of OA based on the spectral signatures corresponding to bio-molecular changes at the cellular level in chondrocytes. In this study, we isolated chondrocytes from human osteoarthritic cartilage and acquired Raman spectra from single cells. Major spectral differences between the cells obtained from different International Cartilage Repair Society (ICRS) grades of osteoarthritic cartilage were identified. During progression of OA, a decrease in protein content and an increase in cell death were observed from the vibrational spectra. Principal component analysis and subsequent cross-validation was able to associate osteoarthritic chondrocytes to ICRS Grade I, II and III with ...
HORIBA Scientific, the global leader in Raman spectroscopy systems and solutions, announces its newest Raman instrument. The AnywhereRaman instrument is a self-contained product that combines a spectrometer, a laser, a ball probe and an acquisition computer all in one instrument, to easily obtain high quality Raman measurements without the need for multiple instrumentation or processes.. HORIBAs new Raman product is designed for both portability and ease of use. It can go from being unpacked and set up, to first Raman measurements in under 20 minutes. These high quality Raman measurements can be obtained by operators with minimal training or experience.. The ball probe with TouchRaman™ immersion technology lets users simply touch the sample, and acquire data. The results are high-quality, reproducible measurements. Its excitation wavelength is 785 nm, with a spectral range of 150- 3150 cm-1.. The all-in-one AnywhereRaman unit is just 3.0 (H) x 9.8 (W) x 9.8 (D), and offers Class 3B laser ...
Methods: Twenty healthy subjects and 20 cancer patients undergoing chemotherapy were included. Measurement time points in cancer patients were before the first cycle of chemotherapy (Tbase) and immediately after intravenous application of the chemotherapy (T1). Healthy subjects were measured once without any further intervention. Measurements were conducted using an individually manufactured system consisting of a handheld probe and a wavelength-tunable diode laser-based 488 nm SHG light source. Hereby, changes in both skin fluorescence and shifted excitation resonance Raman difference spectroscopy (SERRDS) carotenoid signals were assessed ...
Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds up to video-rate, but it sacrifices chemical specificity in samples with overlapping bands compared to broadband (multiplex) excitation. We develop a rapidly tunable picosecond optical parametric oscillator with an electro-optical tunable Lyot filter, and demonstrate multicolor SRS microscopy with synchronized line-by-line wavelength tuning to avoid spectral artifacts due to sample movement. We show sensitive imaging of three different kinds of polymer beads and live HeLa cells with moving intracellular lipid droplets.. © 2013 Optical Society of America. Full Article , PDF Article ...
Scanning electron microscopy with energy dispersive X-ray spectrometry (SEMEDS) and Fourier-transform infrared microspectrophotometry (micro-FT-IR) have been widely demonstrated as complementary analytical tools for the identification of complex mixtures and unknown materials. However, there is a gap between the information provided by these two techniques. ...
We believe that these imaging modalities have many potential medical applications for corneal/ocular surface imaging when the technology is further developed. Chronic topical glaucoma therapy has been associated with corneal damage characterized by the loss of certain corneal cell types as well as invasion of inflammatory cells. 48,49 CARS/TPAF would allow us to observe the number and type of corneal epithelial cells, which could determine the progression of corneal surface disease to better aid physician treatment. CARS/TPAF also has application in the area of corneal surgery, since this technology could be used to observe cell number and metabolic state (NAD[P]H level) at the site of a healing wound. Furthermore, CARS/TPAF can image at the level of the corneal endothelium to monitor cell health before and after cataract surgery. A combined CARS/TPAF system has recently been used to show the differences in lipid structures between in healthy and psoriasis-affected human skin. 50 By the same ...
Synthesis of new proteins, a key step in the central dogma of molecular biology, has been a major biological process by which cells respond rapidly to environmental cues in both physiological and pathological conditions. However, the selective visualization of a newly synthesized proteome in living systems with subcellular resolution has proven to be rather challenging, despite the extensive efforts along the lines of fluorescence staining, autoradiography, and mass spectrometry. Herein, we report an imaging technique to visualize nascent proteins by harnessing the emerging stimulated Raman scattering (SRS) microscopy coupled with metabolic incorporation of deuterium-labeled amino acids. As a first demonstration, we imaged newly synthesized proteins in live mammalian cells with high spatial-temporal resolution without fixation or staining. Subcellular compartments with fast protein turnover in HeLa and HEK293T cells, and newly grown neurites in differentiating neuron-like N2A cells, are clearly ...
We present a microscopic theory of quantum-size effects in surface-enhanced Raman scattering (SERS) from molecules adsorbed on small metal nanoparticles. In noble-metal nanoparticles, the confining potential has different effect on s-band and d-band electrons. Namely, the spillout of delocalized sp-electrons beyond the classical nanoparticle boundary results in an incomplete embedding of s-electron distribution in the background of localized d-electrons whose density profile follows more closely the classical shape. We demonstrate that a reduction of d-electron screening in the surface layer leads to the enhancement of the surface plasmon local field acting on a molecule located in a close proximity to metal surface. Our numerical calculations of Raman enhancement factor, performed using time-dependent local density approximation, show additional enhancement of the Raman signal which becomes more pronounced for small nanoparticles due to the larger ratio of surface layer to overall nanoparticle size.
Resonance Raman spectra of polymer chains in the single crystals of the diacetylenes FBS (2,4-hexadiynylene-di-p-fluorobenzene sulfonate), TS/FBS [6-(p-toluenesulfonyloxy)-2,4-p-fluorobenzene sulfonate] and TS6 (2,4-hexadiynylene-di-p-toluene sulfonate) are described theoretically by means of a Franckâ€Condon model, which considers a chain length dependence. The electronic transition energies and matrix elements are calculated by means of a linear-combination-of-atomic-orbitalsâ€molecular-orbital method calculation in the Hückel approach. We are able to describe the Raman excitation profiles as well as the Raman band profiles for two Raman active modes, i.e., the C+C-, and the C 3/4 C-stretching vibrations of the polymer chains. The model also contains a description of a side group vibrational mode which is enhanced by Fermi resonance with the C=C-stretching vibration. Observed Raman excitation profiles can be well simulated by these calculations. An evaluation of the parameters shows ...
In addition, confocal measurements can improve the rejection of stray light and reduce fluorescence interference. Not all confocal Raman microscopy designs are the same. Traditional confocal Raman microscopes, shown schematically in Figure 1(b), utilize a pinhole aperture placed in front of the spectrograph entrance slit. The Raman light is focused onto the pinhole and the diverging beam after the pinhole is then refocused onto the entrance slit of the spectrograph. Different pinhole apertures can be used to control the degree of confocality, while the entrance slit is used to control the spectral resolution of the spectrometer. While this true confocal configuration provides independent control of spatial and spectral resolutions, it is very difficult to align and to maintain optimum performance. This is because the beam is focused twice through two very small apertures. In practice, independent control of the two apertures offers little value. Typical slit widths must be less than 100 um to ...
A Raman spectrometry apparatus that is capable of measuring and compensating for variabilities in the apparatus comprises a source of substantially monochromatic radiation, means for simultaneously interfacing the radiation with a sample and a reference material, means for simultaneously acquiring at more than one wavelength a convolved Raman spectrum of the sample and a convolved spectrum of the reference material, and means for determining the convolution function of the convolved spectra and applying the convolution function to adjust the convolved Raman spectrum of the sample to produce thereby the standard Raman spectrum of the sample. A method for obtaining the standard Raman spectrum of a sample comprises: (a) simultaneously irradiating the sample and a reference material with a substantially monochromatic radiation source; (b) simultaneously acquiring at more than one wavelength a convolved Raman spectrum of the sample and a convolved spectrum of the reference material; (c) choosing the standard
Metabolic fingerprinting provides valuable information on the physiopathological states of cells and tissues. Traditional imaging mass spectrometry and magnetic resonance imaging are unable to probe the spatial-temporal dynamics of metabolites at the subcellular level due to either lack of spatial r …
As a superb tool to visualize and study the spatial-temporal distribution of chemicals, Raman microscopy has made a big impact in many disciplines of science. While label-free imaging has been the prevailing strategy in Raman microscopy, recent development and applications of vibrational/Raman tags, particul Recent Review Articles
Elkordy, Amal, Forbes, Robert T and Barry, Brian W (2008) Study of protein conformational stability and integrity using calorimetry and FT-Raman spectroscopy correlated with enzymatic activity. European Journal of Pharmaceutical Sciences, 33 (2). pp. 177-190. ISSN 0928-0987 ...
A thermoelectrically cooled surface-enhanced Raman Spectroscopy (TEC-SERS) fiber optic probe for real-time and in-situ monitoring of volatile organic compounds in gas, liquid, and soil environments. The TEC-SERS probe comprises a sample chamber for receiving a gas sample and a fiber optic Raman probe. The sample chamber comprises an inlet having a semipermeable membrane for separating moisture from the gas sample, a SERS substrate mounted on a thermoelectric cooler, a mass flow device for providing airflow, and an output port. The fiber optic Raman probe is operably coupled to a transparent window in the sample chamber for directing an optical excitation signal to irradiate the SERS substrate and for receiving a SERS optical signal from analytes from the gas sample that are in contact with the SERS substrate.
In situ surface-enhanced Raman scattering studies of the nitrosyl adduct of hemin adsorbed roughened silver surfaces in aqueous electrolytes
TY - JOUR. T1 - Present and Future of Surface-Enhanced Raman Scattering. AU - Langer, Judith. AU - Jimenez De Aberasturi, Dorleta. AU - Aizpurua, Javier. AU - Alvarez-Puebla, Ramon A.. AU - Auguié, Baptiste. AU - Baumberg, Jeremy J.. AU - Bazan, Guillermo C.. AU - Bell, Steven E.J.. AU - Boisen, Anja. AU - Brolo, Alexandre G.. AU - Choo, Jaebum. AU - Cialla-May, Dana. AU - Deckert, Volker. AU - Fabris, Laura. AU - Faulds, Karen. AU - García De Abajo, F. Javier. AU - Goodacre, Royston. AU - Graham, Duncan. AU - Haes, Amanda J.. AU - Haynes, Christy L.. AU - Huck, Christian. AU - Itoh, Tamitake. AU - Käll, Mikael. AU - Kneipp, Janina. AU - Kotov, Nicholas A.. AU - Kuang, Hua. AU - Le Ru, Eric C.. AU - Lee, Hiang Kwee. AU - Li, Jian Feng. AU - Ling, Xing Yi. AU - Maier, Stefan A.. AU - Mayerhöfer, Thomas. AU - Moskovits, Martin. AU - Murakoshi, Kei. AU - Nam, Jwa Min. AU - Nie, Shuming. AU - Ozaki, Yukihiro. AU - Pastoriza-Santos, Isabel. AU - Perez-Juste, Jorge. AU - Popp, Juergen. AU - Pucci, ...
Vol 9: Performance-Enhancing Methods for Au Film over Nanosphere Surface-Enhanced Raman Scattering Substrate and Melamine Detection Application.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
We have introduced an in-situ Raman monitoring technique to investigate the crystallization process inside protein drops. In addition to a conventional vapour-diffusion process, a novel procedure which actively stimulates the evaporation from a protein drop during crystallization was also evaluated, with lysozyme as a model protein. In contrast to the conventional vapour-diffusion condition, the evaporation-stimulated growth of crystals was initiated in a simple dehydration scheme and completed within a significantly shorter time. To gain an understanding of crystallization behaviours under the conditions with and without such evaporation stimulation, confocal Raman spectroscopy combined with linear regression analysis was used to monitor both lysozyme and HEPES buffer concentrations in real time. The confocal measurements having a high spatial resolution and good linear response revealed areas of local inhomogeneity in protein concentration when the crystallization started. The acquired ...
This paper reports the first vibrational Raman optical activity (ROA) spectrum of a glycoprotein. The sample, orosomucoid (α1-acid glycoprotein), shows ROA bands characteristic of a high β-sheet content together with new bands which could be specific for the carbohydrate and its association with the protein. Our results suggest that ROA spectra of intact glycoproteins may contain information about both protein and carbohydrate conformation and the mutual influence on each others stability and conformation. ...
The formation of Ni silicides has been successfully monitored by Raman spectroscopy. Ni silicides formed at different annealing temperatures using rapid thermal annealing were analyzed using Rutherford backscattering spectroscopy and X-ray diffraction. Raman spectroscopy was further used to examine these samples. The results showed that Raman spectroscopy could accurately identify the phases of Ni silicides formed at various temperatures. These findings were used to demonstrate the increased thermal stability of NiSi by the addition of Pt. This study demonstrates the applicability of Raman spectroscopy for monitoring the formation of NiSi, which was suggested to be the future silicide for deep submicrometer integrated circuit processing. Raman spectroscopy offers a unique tool for phase identification at localized areas and mapping characterization of Ni silicides with micrometer spatial resolution ...
Christian Knipfer,1,3,* Johanna Motz,1 Werner Adler,4 Kathrin Brunner,5 Medhaine Tesfay Gebrekidan,2,3 Robert Hankel,2,3 Abbas Agaimy,5 Stefan Will,2,3 Andreas Braeuer,2,3 Friedrich Wilhelm Neukam,1,3 and Florian Stelzle1,3 1Department of Oral and Maxillofacial Surgery, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Erlangen, Germany 2Lehrstuhl für Technische Thermodynamik, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Erlangen, Germany 3Erlangen Graduate School in Advanced Optical Technologies (SAOT), Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Erlangen, Germany 4Department of Medical Informatics, Biometry and Epidemiology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Erlangen, Germany 5Department of Pathology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Erlangen, Germany *Corresponding author: [email protected] ...
G-Quadruplex (G4) structures of a human telomeric 24-mer (5′-TTAGGGTTAGGGTTAGGGTTAGGG-3′) sequence (Tel24) stabilized by sodium and potassium ions have been assessed using surface-enhanced Raman scattering (SERS) spectroscopy. The distinctive SERS spectra of Tel24 in the presence of 100 mM Na+ and 100 mM K+ were obtained and the SERS bands characteristic of the antiparallel baskettype and the mixed hybrid (3+1) structures, respectively, were identified and assigned. The influence of the SERS - active substrate on the scattering enhancement was studied using citrate- and chloride-covered silver nanoparticles, in the absence and presence of the aggregating agent (0.1 M Na2SO4 and 0.1 M K2SO4). The highly reproducible SERS spectra of Tel24 obtained in various SERS active media indicated the same adsorption mechanism of the cation - stabilized G-quadruplexes onto the metal surface, regardless of the silver colloid. The remarkable resemblance between the circular dichroism (CD) spectra of the ...
Human telomeric G-quadruplexes are emerging targets in anticancer drug discovery since they are able to efficiently inhibit telomerase, an enzyme which is
ABSTRACT-UV-visible reflectance spectroscopy and Raman microspectroscopy provide a rapid way to unequivocally identify the pre-Columbian pigment Maya blue. Spectra of modern synthetic materials are compared with data from a contextualized archaeological sample and from an object in the collection of the Los Angeles County Museum of Art. UV-visible spectroscopy and Raman microspectroscopy, together with complementary techniques such as Fourier transform infrared microspectroscopy, reveal significant differences between spectra of indigo as pure crystalline solid or as complexed by palygorskite. These techniques are thus extremely specific, being able to identify Maya blue as a prepared pigment rather than detecting only its ingredients, indigo and palygorskite. Fiber optics UV-visible reflectance spectroscopy and Raman microspectroscopy present the additional advantage of being completely noninvasive and therefore suitable for the study of works of art. Lightweight portable fiber optics ...
Two-photon polymerization (TPP) is an enabling technology that allows fast prototyping of parts with sub-100 nm resolution. Due to its ability to fabricate microstructures with arbitrary three-dimensional geometries, TPP has been employed in diverse fields such as nanophotonics, microelectronics, microelectromechanical systems, and microfluidics. However, no information is available to date that microscopically correlates the experimental conditions used in TPP with the properties of the ultimate microstructure. We present a study where the distribution of polymer cross-linking in three-dimensional microstructures fabricated by TPP is visualized by means of coherent anti-Stokes Raman scattering (CARS) microscopy. The characterization of the microstructures based on the acquired images permits rational optimization of the TPP process
TY - JOUR. T1 - Resonance Raman spectra of ferrochelatase reveal porphyrin distortion upon metal binding. AU - Blackwood, Milton E.. AU - Rush, Thomas S.. AU - Medlock, Amy Elizabeth. AU - Dailey, Harry A.. AU - Spiro, Thomas G.. PY - 1997/12/17. Y1 - 1997/12/17. N2 - Ferrochelatase catalyzes Fe2+ insertion into porphyrins, and is inhibited by Hg2+. Resonance Raman spectra of mesoporphyrin IX show that binding to ferrochelatase restricts the conformation of the propionate side chains, but does not perturb the ring conformation. However, a pronounced perturbation is seen in the ternary complex with Hg2+. Several additional RR bands are activated, including some arising from [R-active vibrations, establishing loss of an effective symmetry center. Out-of-plane modes appear in the low frequency region. The strongest of these bands, γ5 and γ6, correspond to pyrrole tilting vibrations, which are in the same symmetry class as a doming distortion of the porphyrin. All four pyrrole N atoms are pointing ...
Resonance Raman spectroscopy was used to probe the effect of solvent polarity on the molecular structure and isomerization dynamics of $4-nitro-4^\prime -dimethylaminoazobenzene$ (DA). In addition, the influence of the polarity and the relaxation behavior of the solvent on the mode-specific vibrational and solvent reorganization energies was investigated. Raman spectra were recorded in solvents with different polarity parameters, n-hexane and benzene, using 11 excitation wavelengths in the range 450-550 nm, scanning every 10 nm using the tunable laser pulse output of an optical parametric oscillator. It was observed that the solvent polarity plays a major role in influencing the excited-state potential energy surfaces of DA. The plot of resonance Raman intensities versus excitation wavelength (Raman excitation profiles) of the Franck-Condon-active fundamentals of DA shows that in n-hexane, the locally excited state is more favorable than that in benzene where the charge-transfer state is more ...
Nanoporous thin films with silver nanoparticles were synthesized with a bottom-up approach, and its potential as effective surfaceenhanced Raman scattering (SERS) substrates was demonstrated. The use of mesoporous titania films as substrates allowed to control the growth of nanoparticles on the film surface. Atomic force microscopy measurements, Ultraviolet-visible and X-ray diffraction analysis confirmed the photoreduction of Ag+ to Ag0 with the formation of nanoparticles with crystallite dimensions of 32 to 36 nm. The new substrates allowed the detection of two analytes (rhodamine B isothiocyanate and cytochrome c), present in solutions at very low concentrations, highlighting their potential in SERS sensing. Reproducibility, homogeneity, enhancement factor of the substrate, consistency of results and detection limits were also assessed. ...
Objective: Regeneration of the CNS and PNS cannot be monitored until we have a final functional readout: movement or paresis. Therefore, there is an urgent need for in vivo imaging modalities to analyse and control therapeutic impact after damage to the nervous system. We investigated time-dependent morphochemical tissue changes after CNS and PNS injury in the axolotl by label-free multiphoton microscopy (MPM).. Method: Two models of injury were investigated: spinal cord transection or sciatic nerve transection, cryosections of the injured area were prepared at 0d, 2d, 7d, 14d, 24d, 28d, 42d and 100d post-lesioning (n=10 per time point) and analyzed with MPM. Specifically, coherent anti-stokes Raman scattering (CARS) probed lipid-rich myelin via visualization of CH2 vibrations, while two photon excited fluorescence (TPEF) and second harmonic generation (SHG) displayed endogenous fluorophores and collagen, respectively. Immunostaining for myelin basic protein, neurofilament and ionized calcium ...
While there have been many publications produced in which Raman spectroscopy has been used to characterize various aspects of carbon nanostructures, there have been few works published which provide an overview of what the technique can offer to the field of carbon nanostructures, and works which discuss optimization of analysis parameters and the variation that is observed as those parameters are varied are still lacking. This poster will provide an overview of the information that Raman spectroscopy can provide on carbon nanostructures with emphasis placed on carbon nanotubes and graphene. This work will go further to demonstrate the impact that varying key analysis parameters such as laser power will have on the measurements. The goal of this poster is to provide people who are looking to characterize carbon nanostructures with an understanding of what Raman spectroscopy can offer and with some insight on how to optimize measurement conditions.
While there have been many publications produced in which Raman spectroscopy has been used to characterize various aspects of carbon nanostructures, there have been few works published which provide an overview of what the technique can offer to the field of carbon nanostructures, and works which discuss optimization of analysis parameters and the variation that is observed as those parameters are varied are still lacking. This poster will provide an overview of the information that Raman spectroscopy can provide on carbon nanostructures with emphasis placed on carbon nanotubes and graphene. This work will go further to demonstrate the impact that varying key analysis parameters such as laser power will have on the measurements. The goal of this poster is to provide people who are looking to characterize carbon nanostructures with an understanding of what Raman spectroscopy can offer and with some insight on how to optimize measurement conditions.
Eggeling, C.; Schaffer, J.; Seidel, C.; Korte, J.; Brehm, G.; Schneider, S.; Schrof, W.: Homogeneity, transport and signal properties of single Ag particles studied by single-molecule surface-enhanced resonance Raman scattering. Journal of Physical Chemistry A 105, pp. 3673 - 3679 (2001 ...
Research in the Fu group focuses on developing novel optical spectroscopy and imaging techniques to investigate the spatial-temporal dynamics of living biological systems at single cell resolution. In particular, we are interested in using label-free spectroscopic imaging approaches such as stimulated Raman scattering microscopy to study the cellular mechanism of complex diseases such as cancer and metabolic disorders. We draw on expertise from multiple disciplines including analytical chemistry, ultrafast spectroscopy, imaging science, optical engineering, bioinstrumentation, cell biology, and systems biology.. Label-free optical microscopy. Optical microscopy, especially fluorescence microscopy, is instrumental in unraveling the intricate biological processes that happen inside living cells. However, most biomolecules in the cell do not fluorescence and visualizing those molecules requires fluorescent tags, which may often be unavailable or perturbative to their biological functions. We ...
Microthermometry is the most common technique used to determine the salinity of aqueous fluid inclusions but, in some cases, this technique cannot be applied. The Raman analysis of water is a good alternative. Instead of preparing fluid inclusions within minerals by the crack-and-heal method, fused-silica capillary capsules (FSCCs) were used as synthetic fluid inclusions to calibrate the Raman signal of water toward chlorinity. The preparation of FSCCs is convenient in the preparation of synthetic fluid inclusions, and the composition of the standard solutions is well controlled all along the preparation procedure. Standard fluid compositions made in FSCCs are stable over time. The treatment of Raman data is reduced to the subtraction of a straight baseline and the measurement of Raman intensity at two wavenumbers, such that it is simple and independent from the operator. The method has been tested by comparison of the results obtained from Raman spectroscopy and with phase transitions observed ...
Dr. Tuan Vo-Dinh is R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering, Professor of Chemistry, and Director of The Fitzpatrick Institute for Photonics.. Dr. Vo-Dinhs research activities and interests involve biophotonics, nanophotonics, plasmonics, laser-excited luminescence spectroscopy, room temperature phosphorimetry, synchronous luminescence spectroscopy, surface-enhanced Raman spectroscopy, field environmental instrumentation, fiberoptics sensors, nanosensors, biosensors and biochips for the protection of the environment and the improvement of human health.. Dr. Vo-Dinhs research activities and interests include the development of advanced technologies and methods in biophotonics, nanophotonics, biosensors, biochips, plasmonics, multi-modality bioimaging, and theranostics (diagnostics and therapy) of diseases such as cancer and infectious diseases. We have pioneered the development of a new generation of gene probes using surface-enhanced Raman scattering ...
Dr. Tuan Vo-Dinh is R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering, Professor of Chemistry, and Director of The Fitzpatrick Institute for Photonics.. Dr. Vo-Dinhs research activities and interests involve biophotonics, nanophotonics, plasmonics, laser-excited luminescence spectroscopy, room temperature phosphorimetry, synchronous luminescence spectroscopy, surface-enhanced Raman spectroscopy, field environmental instrumentation, fiberoptics sensors, nanosensors, biosensors and biochips for the protection of the environment and the improvement of human health.. Dr. Vo-Dinhs research activities and interests include the development of advanced technologies and methods in biophotonics, nanophotonics, biosensors, biochips, plasmonics, multi-modality bioimaging, and theranostics (diagnostics and therapy) of diseases such as cancer and infectious diseases. We have pioneered the development of a new generation of gene probes using surface-enhanced Raman scattering ...
CRAIC Technologies has developed a new solution for the semiconductor industry: the 20/20 XL™ Film Thickness Measurement Tool. The 20/20 XL™ is a microspectrophotometer is designed to non-destructively analyze microscopic areas of very large samples. This system offers the ability to measure the thickness of thin films in both transmission and reflectance.
The characteristic AgcoreAushell nanoparticles as SERS substrates in detecting dopamine molecules at various pH ranges Yanru Bu,1 Sang-Wha Lee2 1Department of Chemical Engineering, Faculty of Engineering, Monash University, Melbourne, VIC, Australia; 2Department of Chemical and Biochemical Engineering, Gachon University, Seongnam-si, Gyeonggi-do, Republic of Korea Abstract: AgcoreAushell nanoparticles (NPs) are a promising surface-enhanced Raman scattering (SERS) substrate, which can offer a high enhancement factor through the combined effect of the high SERS activity of the Ag core and the biocompatibility of the Au shell. In this study, AgcoreAushell NPs were examined as SERS substrates for the sensitive detection of dopamine (DA) molecules in an aqueous solution. The SERS activity of the AgcoreAushell NPs was strongly dependent on the pH of the solution. When the pH of the solution was acidic (pH 9), the AgcoreAushell NPs exhibited negligible SERS activity toward the DA molecules, due to the
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a set of infrared and Raman spectra of 500 common and artificial polymers of commercial value is gifted during this publication. a wide number of compounds are incorporated, beginning with linear polyolefins and completing with complicated biopolymers and similar compounds. The spectra have been registered utilizing Infrared Fourier remodel Spectrometers within the laboratory of the All-Russia Institute of Forensic Sciences. The IR and Raman spectra are awarded jointly at the related sheet. The accompanying information comprise normal and constitution formulae, CAS sign in numbers, and pattern training conditions.. Features of this book:. • keeps the lengthy culture of publishing particular and traditional info of latest chemical compounds.. • For low-molecular weight ingredients, complementary IR and Raman spectra are featured at the comparable pattern and imprinted on the comparable web page. This fingerprint info permits the substance of the pattern to be pointed out with no ...
Very small, thioglycerol (TG)-capped CdS nanoparticles were synthesized by a wet chemical technique and investigated in the framework of this thesis. Also glutathione-capped particles were investigated for a comparison of the capping agents. High-resolution photoelectron spectroscopy using high-brilliance synchrotron radiation was applied as the major tool for the characterization of these particles. Additionally, the particles were investigated with UV-VIS absorption spectroscopy, XPS using a laboratory source, valence band photoemission spectroscopy (VBPES), near-edge x-ray absorption spectroscopy (NEXAFS), and micro-Raman spectroscopy to address various aspects of the particles. In the beginning, an overview on size quantization effects is given to create a theoretical background behind the work presented in this thesis. Furthermore, an overview of various conventional techniques for size determination is presented. Exact information about size, shape and size distribution of nanoparticles is ...
Retinoic acid (RA) is essential for early developmental processes and stem cell differentiation, but less is known about its contributions to adult tissues and stem cells including adipose tissue. We previously demonstrated that many genes involved in RA synthesis and downstream pathway are differentially expressed in adipose-derived stem cells (ASCs) from visceral fat compared to those from subcutaneous fat, leading to changes in their early adipogenic functions. In order to study potential contributions of RA in adipose tissue, we measured tissue RA levels using a technique based on surface-enhanced Raman spectroscopy (SERS). The data indicate heretofore underappreciated abundance of endogenous RA in mouse adipose tissue compared to other tissues and dynamic changes of RA concentrations after high fat diet feeding. Our results lay the foundation for further investigation on the functional role of RA in adipose tissue development and metabolism ...
The resonance Raman (RR) spectra of six types of human brain tissues are examined using a confocal micro-Raman system with 532-nm excitation in vitro. Forty-three RR spectra from seven subjects are investigated. The spectral peaks from malignant meningioma, stage III (cancer), benign meningioma (benign), normal meningeal tissues (normal), glioblastoma multiforme grade IV (cancer), acoustic neuroma (benign), and pituitary adenoma (benign) are analyzed. Using a 532-nm excitation, the resonance-enhanced peak at 1548 cm−1 (amide II) is observed in all of the tissue specimens, but is not observed in the spectra collected using the nonresonance Raman system. An increase in the intensity ratio of 1587 to 1605 cm−1 is observed in the RR spectra collected from meningeal cancer tissue as compared with the spectra collected from the benign and normal meningeal tissue. The peak around 1732 cm−1 attributed to fatty acids (lipids) are diminished in the spectra collected from the meningeal cancer tumors ...
The influence of porosity on the superconducting properties have been investigated on YBa/sub 2/Cu/sub 3/O/sub 7/ thin films deposited on LaAlO/sub 3/ [100] substrates by the so-called Trifluoroacetate (TFA) route. Micro-Raman spectroscopy have been used to determine the concentration of c-axis grains /spl delta/ in different samples and their influence on the final film porosity as observed from SEM imaging. This has been compared with measurements of resistivity and critical currents in the same samples. We prove that this /spl delta/ fraction is the main parameter controlling the porosity and hence the normal-state resistivity of the thin films. The optimization of the microstructure of these YBa/sub 2/Cu/sub 3/O/sub 7/ TFA films allow to have high critical currents : J/sub c/ = 3 /spl times/ 10/sup 6/ A/cm/sup 2/ at 77 K ...
Quantum chemical calculations on the geometrical, conformational, spectroscopic (FTIR, FT-Raman) analysis and NLO activity of milrinone [5-cyano-2-methyl-(3,4-bipyridin)-6(1h)-one] by using hartree-fock and density functional methods
Differential reflectivity (ZDR) and circular depolarization ratio (CDR) radar signals are directly related to each other and to the median drop size diameter of rainfall when the drop sizes are exponentially distributed. These polarization parameters are examined for both static and oscillating raindrop shapes along with their relationships with rainfall rate and liquid water content estimates derived from ZDR or CDR and reflectivity factor (Z) measurements. Propagation effects at S band wavelengths are also considered and shown to be particularly important in CDR measurements. ...
Therapeutic and subtherapeutic use of veterinary drugs has increased the risk of residue contamination in animal food products. Antibiotics such as tetracycline are used for mastitis treatment of lactating cows. Milk expressed from treated cows before the withdrawal period has elapsed may contain tetracycline residue. This study developed a simple surface-enhanced Raman spectroscopic (SERS) method for on-site screening of tetracycline residue in milk and water. Six batches of silver colloid nanoparticles were prepared for surface enhancement measurement. Milk-tetracycline and water-tetracycline solutions were prepared at seven concentration levels (1000, 500, 100, 10, 1, 0.1, and 0.01 ppm) and spiked with silver colloid nanoparticles. A 785 nm Raman spectroscopic system was used for spectral measurement. Tetracycline vibrational modes were observed at 1285, 1317 and 1632 cm−1 in water-tetracycline solutions and 1322 and 1621 cm−1 (shifted from 1317 and 1632 cm−1, respectively) in milk-tetracycline
TY - GEN. T1 - Effect of silver concentration towards formation of agpt nanofern films as sers substrates. AU - Norhayati, Abu Bakar. AU - Nur, Adliha Abdullah. AU - Akrajas, Ali Umar. AU - Mat Salleh, Muhamad. AU - Joseph, George Shapter. PY - 2019/1/1. Y1 - 2019/1/1. N2 - This paper reports the preparation of silver-platinum (AgPt) nanoferns on ITO surface using liquid phase deposition technique with various concentrations of silver nitrate in synthesis solution. The different morphologies of AgPt thin films were grown on the surface using 0.1 mM to 0.8 mM of silver concentration. This silver effect on the growth of AgPt on the surface was studied using FESEM and UV-Vis characterization. It was found that the optimum silver concentration in synthesis solution supplied the appropriate Ag+ ion to grow the nanoferns structure on the surface. AgPt films were then carried out to sense 1.0 M of creatinine concentration to learn their performance as surface-enhanced Raman scattering (SERS) substrate. ...
Raman spectroscopy has emerged as a promising tool for real-time clinical diagnosis of malignant skin tumors offering a number of potential advantages: it is non-intrusive, it requires no sample preparation, and it features high chemical specificity with minimal water interference. However, in vivo tissue evaluation and accurate histopathological classification remain a challenging task for the successful transition from laboratory prototypes to clinical devices. In the literature, there are numerous reports on the applications of Raman spectroscopy to biomedical research and cancer diagnostics. Nevertheless, cases where real-time, portable instrumentations have been employed for the in vivo evaluation of skin lesions are scarce, despite their advantages in use as medical devices in the clinical setting. This paper reviews the advances in real-time Raman spectroscopy for the in vivo characterization of common skin lesions. The translational momentum of Raman spectroscopy towards the clinical practice
3.0.CO;2-T. Dybal, J.; Krimm, S. (1990). "Normal-mode analysis of infrared and Raman spectra of crystalline isotactic poly( ... In cases of limited resolution stochastic methods such as Bernoullian or Markovian analysis may also be used to fit the ... CS1 maint: discouraged parameter (link) Paukkeri, R; Vaananen, T; Lehtinen, A (1993). "Microstructural analysis of ... Surface and Interface Analysis. 25: 41. doi:10.1002/(SICI)1096-9918(199701)25:1. ...
... coordinated analyses of Raman spectra, reflectance spectra and elemental abundances". Philos Trans Royal Soc A. 372 (2030). doi ... "Mineralogical analyses of surface sediments in the Antarctic Dry Valleys: ...
... thus allowing measurement and analysis of their Raman spectra. Cathodoluminescence (CL) - light emission excited by electron ... are frequently seen in CNT Raman spectra. However, they are less important and are not considered here. All the above Raman ... For example, Raman spectra are extremely difficult to measure from dyes because of their strong PL (efficiency close to 100%). ... 2006). "Vibrational Analysis of Organic Molecules Encapsulated in Carbon Nanotubes by Tip-Enhanced Raman Spectroscopy". ...
They also showed that the analysis for the Raman spectra of SWNTs is consistent with general understanding on Raman analysis of ... reinterpreted SWNTs to be a graphene helix via Raman spectroscopy, showing that the typical Raman spectrum for SWNTs is the ... "Reinterpretation of Single-Wall Carbon Nanotubes by Raman Spectroscopy". The Journal of Physical Chemistry C. xx (22): 14003- ... signature of their helical structure with density functional theory simulation and structure analysis for hydrogenated and ...
... thus allowing measurement and analysis of their Raman spectra. Squaric acid Sprenger, H. E. & Ziegenbein, W. (1966). " ... 2006). "Vibrational Analysis of Organic Molecules Encapsulated in Carbon Nanotubes by Tip-Enhanced Raman Spectroscopy". Jpn. J ...
Beginning with his dissertation work on Raman spectroscopy, he worked on the analysis of vibrational spectra of cyclic ... He was a co-author of Course Notes on the Interpretation of Infrared and Raman Spectra (2004). He co-edited Spectrochimica Acta ... Among other publications, he co-authored the books Course Notes on the Interpretation of Infrared and Raman Spectra (2004) and ... His dissertation was The raman spectra of pyrrole and some of its deuterium derivatives. Miller held a post-doctoral fellowship ...
Raman was Director and with the facilities provided there, they further extended their work on analysis of spectra in the ... Raman scattering, Fluorescence and Phosphorescence and Crystal Spectra. He also reached the level of construction of microwave ... A.Fowler at the Imperial College of Science and Technology, London in 1930 in Atomic Spectra for two years for which he was ... Some of his initial publications are given below On the spectra of the metals of the aluminium sub-group, Proceedings of the ...
... and Raman spectra), although its application is not limited to spectroscopy. 2D correlation analysis is frequently used for its ... The original spectra are compared to a reference spectrum. By subtracting a reference spectrum, often the average spectrum of ... The measured signals or spectra will show systematic variations that are processed with 2D correlation analysis for ... Hence, interpretation begins with studying the autocorrelation spectrum on the main diagonal of the 2D synchronous spectrum. In ...
"Infrared and Raman spectra of the hexaaquaruthenium ions: normal-coordinate analysis for Ru ( H 2 O ) 6 2 + {\displaystyle {\ce ... Handbook of Infrared and Raman Spectra of Inorganic Compounds and Organic Salts: Infrared Spectra of Inorganic Compounds. ... Ananthanarayanan, V. (1961). "Raman spectra of crystalline double sulphates". Zeitschrift für Physik. 163 (2): 144-157. Bibcode ... Ananthanarayanan, V. (June 1962). "Raman spectra of crystalline double sulphates Part II. Ammonium double sulphates". ...
... spectrum analysis, raman MeSH E05.196.922.250 - colorimetry MeSH E05.196.922.375 - complement hemolytic activity assay MeSH ... actuarial analysis MeSH E05.318.740.150 - analysis of variance MeSH E05.318.740.150.500 - multivariate analysis MeSH E05.318. ... sequence analysis, dna MeSH E05.393.760.700.300 - dna mutational analysis MeSH E05.393.760.705 - sequence analysis, protein ... discriminant analysis MeSH E05.318.740.400 - factor analysis, statistical MeSH E05.318.740.475 - matched-pair analysis MeSH ...
Raman spectroscopy employs the Raman effect for substances analysis. The spectrum of the Raman-scattered light depends on the ... At high pumping levels in long fibers, higher-order Raman spectra can be generated by using the Raman spectrum as a new ... The spectrum of the scattered photons is termed the Raman spectrum. It shows the intensity of the scattered light as a function ... Inverse Raman effect[edit]. The inverse Raman effect is a form of Raman scattering first noted by W. J. Jones and Boris P. ...
Multivariate analysis of Raman spectra has enabled development of a quantitative measure for wound healing progress.[19] ... The advanced types of Raman spectroscopy include surface-enhanced Raman, resonance Raman, tip-enhanced Raman, polarized Raman, ... and λ1 is the Raman spectrum wavelength. Most commonly, the unit chosen for expressing wavenumber in Raman spectra is inverse ... By using Raman microspectroscopy, in vivo time- and space-resolved Raman spectra of microscopic regions of samples can be ...
McCullough, R.L.; Jones, L.H.; Crosby, G.A. (January 1960). "An analysis of the vibrational spectrum of the tetracyanonickelate ... Raman Spectroscopy, and Structural Characterization of Actinide Tetracyanometallates". Inorganic Chemistry. 52 (9): 4880-4889. ...
"Radio frequency-microwave double resonance as a tool in the analysis of microwave spectra". Journal of Molecular Spectroscopy. ... "The preparation and Raman spectroscopic study of the chlorotetrafluorosilicate (IV) ion, SiF4Cl−". Journal of Molecular ... doi:10.1016/0022-2852(71)90176-7. Sheridan, John; Gordy, Walter (March 1950). "Microwave Spectra and Molecular Constants of ... Hasegawa, Akinori; Uchimura, Schunichiro; Koseki, Kohji; Hayashi, Michiro (January 1978). "ESR spectrum and structure of the ...
It has been developed mainly aiming to Raman spectra analysis, but since it works with 2-columns datafiles (Intensity vs ... Raman Tool Set is a free software package for processing and analysis of Raman spectroscopy datasets. ... Beyond the spectra preprocessing steps, such as baseline subtraction, normalization of spectra, smoothing and scaling, Raman ... independent component analysis (ICA) and cluster analysis. Raman Tool Set is currently developed at BioNEM Lab of the ...
The acquisition of a large number of such single-shot spectra also has a critical role in these analyses. This measurement ... Such a soliton has short duration and high peak intensity, and Raman scattering ensures that it is also redshifted relative to ... By applying a correlation analysis between the redshifted output energy and the input noise, it was observed that a particular ... Third-order dispersion and Raman scattering play a central role in the generation of large redshifts, and turbulence treats the ...
3.0.CO;2-N. Szybowicz, M (October 2004). "High temperature study of FT-IR and Raman scattering spectra of vacuum deposited CuPc ... "Lock-in phase analysis of copper phthalocyanine photoabsorption spectrum". Optica Applicata. 35 (3): 561-571. Bala, M; Wojdyla ... on Raman, FT-IR, absorbance, reflectance, and photoluminescence spectra". J. Optoelectron. Adv. M. 11 (3): 264-269. Askat E, ...
To obtain a high resolution Raman micrograph/spectrum, the following conditions should be met: (1) the size of the aperture ... Theory and instrumentation of Raman/AFM and IR/AFM combine the theory of SPR (AFM and NSOM) and Raman scattering, and this ... which is the reason why the AFM-Raman combination is preferred for realizing Raman-NSOM. The main drawback of the aperture mode ... The combination of Raman scattering and NSOM techniques was first realized in 1995, when it was used for imaging a Rb-doped KTP ...
When multi-variate techniques, such as principal component analysis are used, it is necessary to take several spectra at varied ... Spatially offset Raman spectroscopy (SORS) is a variant of Raman spectroscopy that allows highly accurate chemical analysis of ... Raman spectroscopy relies on inelastic scattering events of monochromatic light to produce a spectrum characteristic of a ... The two spectra can be subtracted using a scaled subtraction to produce two spectra representing the subsurface and surface ...
By using Raman microspectroscopy, in vivo time- and space-resolved Raman spectra of microscopic regions of samples can be ... Both analysis methods are then performed automatically at the same sample location. The obtained SEM and Raman images can then ... Common examples of correlative microscopy techniques are Raman-AFM, Raman-SNOM, and Raman-SEM. Correlative SEM-Raman imaging is ... By using Raman microspectroscopy, in vivo time- and space-resolved Raman spectra of microscopic regions of samples can be ...
Transmission Raman lends itself to rapid, non-invasive and non-destructive analysis of pharmaceutical dosage forms such as ... Analysing Raman spectra to produce assay results requires a method to separate the individual spectral components and correlate ... This is typically facilitated using chemometric analysis methods. Transmission Raman spectroscopy can be used as a process ... The use of Raman spectroscopy in medical applications has been limited to depths of hundreds of micrometres (confocal Raman). ...
... (LSSA) is a method of estimating a frequency spectrum, based on a least squares fit of ... Korenberg, Michael J.; Brenan, Colin J. H.; Hunter, Ian W. (1997). "Raman Spectral Estimation via Fast Orthogonal Search". The ... Alternatively, magnitudes in the Vaníček spectrum can also be expressed in dB. Note that magnitudes in the Vaníček spectrum ... Scargle, J. D. (1982). "Studies in astronomical time series analysis. II - Statistical aspects of spectral analysis of unevenly ...
... vibrational spectroscopy gaining Raman spectra obtained by use of a Renishaw RM 1000 system, (equipped with Leica DMLM series ... Results of the analyses were presented to IMA Commission on New Minerals and Mineral Names (2001-027) during 2001. The ... Using a combination of X-ray diffraction, with analysis using a 100 mm diameter Guinier-Ha gg camera (using Cr-Kα1 radiation (l ... to perform the required tests and analyses in order to determine the nature, and properties of this (then) new mineral. ...
A material's blackbody spectrum is a spontaneous emission spectrum determined by its temperature. This feature can be measured ... Raman optical activity spectroscopy exploits Raman scattering and optical activity effects to reveal detailed information on ... Dynamic mechanical analysis can be employed to impart radiating energy, similar to acoustic waves, to solid materials. The ... Spectra of atoms and molecules often consist of a series of spectral lines, each one representing a resonance between two ...
Various molecular properties: IR, Raman, VCD, UV, XAS spectra; NMR and EPR (ESR) parameters. Solvent and environmental effects ... Many chemical analysis tools (energy decomposition analysis, transfer integrals, (partial) density of states, etc.) Periodic ...
all data] Morgan, C.E.; Frommhold, L., Raman spectra of van der Waals dimers in argon, Phys. Rev. Lett., 1972, 29, 1053. ... 10.1063/1.438529 Emission spectrum of rare gas dimers in the vacuum UV region. II. Rotational analysis of band system I of Ar2 ... Frommhold, L.; Bain, R., Comments concerning the "Raman spectra of van der Waals dimers in argon", J. Chem. Phys., 1975, 63, ... Issue 8 Interpretation of Raman spectra of van der Waals dimers in argon The Journal of Chemical Physics 61, 2996 (1974); https ...
Raman spectroscopy is a method of chemical analysis that is non-destructive. The analysis provides detailed information about ... Meyrowitzite's Raman spectroscopy was conducted on a Horiba XploRA PLUS. The spectrum was recorded using a 785 nm diode laser ... Meryrowitzites spectrum is similar to that of Zellerites. However, Meyrowitzite's spectrum has a larger number of bands ... Chemical Analysis of Meyrowitzite was performed on a Cameca SX-50 electron microprobe with four wavelength-dispersive ...
"Real-time analysis of metabolic products". phys.org. Retrieved May 20, 2020. Oliver SG, Winson MK, Kell DB, Baganz F (September ... These measurements may be digitized spectra, or a list of metabolite features. In its simplest form this generates a matrix ... Raman spectroscopy and radiolabel (when combined with thin-layer chromatography).[citation needed] The data generated in ... For multivariate analysis, models should always be validated to ensure the results can be generalized. Machine learning is also ...
Different kinds of spectra are often used in chemical spectroscopy, e.g. IR, microwave, NMR, ESR, etc. Spectroscopy is also ... They can be analyzed using the tools of chemical analysis, e.g. spectroscopy and chromatography. Scientists engaged in chemical ... Analytical chemistry is the analysis of material samples to gain an understanding of their chemical composition and structure. ... the analysis of material samples to gain an understanding of their chemical composition and structure. Many more specialized ...
Qualitative analysis can be used and spectrophotometers are used to record spectra of compounds by scanning broad wavelength ... Coherent anti-Stokes Raman spectroscopy. *Cold vapour atomic fluorescence spectroscopy. *Conversion electron Mössbauer ... visible spectrum region of interest), or quartz (Far UV spectrum region of interest). Some applications require small volume ... In astronomy, the term spectrophotometry refers to the measurement of the spectrum of a celestial object in which the flux ...
Raman spectroscopy. Rayleigh scattering. Fluorescence. Phosphorescence. Jablonski diagram. Emission spectrum. Mie scattering. ... Atoms, Ions, Bonds, Molecules, Electronic, and Chemical Analysis; You Don't See that in Dasher![edit]. Pamela Brown[edit]. ... User:Plbrown123/Books/Atoms, Ions, Bonds, Molecules, Electronic, and Chemical Analysis; You Don't See that in Dasher!. From ... Analysis;_You_Don%27t_See_that_in_Dasher!&oldid=604516570" ...
Raman spectrum of liquid 2-mercaptoethanol (below) and SERS spectrum of 2-mercaptoethanol monolayer formed on roughened silver ... "Mixture analysis and quantitative determination of nitrogen-containing organic molecules by surface-enhanced Raman spectrometry ... Additional modes not found in the traditional Raman spectrum can be present in the SERS spectrum, while other modes can ... Some bands appear only in the bulk-phase Raman spectrum or only in the SERS spectrum. ...
X-ray binaries are binary star systems that emit a majority of their radiation in the X-ray part of the spectrum. These X-ray ... A 2016 analysis of LIGO data shows tentative signs of echoes caused by a fuzzy event horizon; such echoes may be possible in ... Secondly, the red shift of the spectral lines would be so great that the spectrum would be shifted out of existence. Thirdly, ... with the same spectrum as a black body of a temperature inversely proportional to its mass. This temperature is on the order of ...
"The Crystal Structure and Raman Spectrum of Ge5Cl12·GeCl4 and the Vibrational Spectrum of Ge2Cl6". Inorg. Chem. 37 (23): 6032- ... Eugene P. Bertin, Principles and practice of X-ray spectrometric analysis, Chapter 5.4-Analyzer crystals, Table 5.1, p. 123; ... 4), while Lecoq de Boisbaudran deduced 72.3 by a comparison of the lines in the spark spectrum of the element.[17] ... A.; Shevyakov, A. M. (1975). "Infrared reflectance and transmission spectra of germanium dioxide and its hydrolysis products". ...
Elemental analysis. *Elliptic flow. *Emission spectrum. *Energetic neutral atom. *Energy density. *Energy filtered transmission ... Surface enhanced Raman spectroscopy. *Surface plasmon. *Surface plasmon polaritons. *Surface plasmon resonance ... SSIES, Special Sensors-Ions, Electrons, and Scintillation thermal plasma analysis package. *SST-1 (tokamak), Steady State ...
The incandescent light bulb has a spectrum overlapping the black body spectra of the sun and the earth. Some of the photons ... Rephaeli, Eden; Raman, Aaswath; Fan, Shanhui (2013). "Ultrabroadband photonic structures to achieve high-performance daytime ... In radiation analysis a surface is defined as smooth if the height of the surface roughness is much smaller relative to the ... Another way to modify the object thermal emission spectrum is by reducing the dimensionality of the emitter itself.[4] This ...
IEEE Spectrum: Electricity Over Glass. IEEE Spectrum. October 2005. *^ Photovoltaic feat advances power over optical fiber - ... Such fiber is called multi-mode fiber, from the electromagnetic analysis (see below). In a step-index multi-mode fiber, rays of ... Smith, R. G. (1972). "Optical Power Handling Capacity of Low Loss Optical Fibers as Determined by Stimulated Raman and ... "Photonics Spectra. Retrieved 2011-01-23.. *^ Seo, Koji; et al. (October 2003). "Evaluation of high-power endurance in optical ...
This is called the visible part of the spectrum.. *^ Buser, Pierre A.; Imbert, Michel (1992). Vision. MIT Press. p. 50. ISBN ... Raman · Bragg · Bardeen · Shockley · Chadwick · Lawrence · Zeilinger · Goudsmit · Uhlenbeck ... Ohannesian, Lena; Streeter, Anthony (9 November 2001). Handbook of Pharmaceutical Analysis. CRC Press. p. 187. ISBN 978-0-8247- ... "Spectrum and the Color Sensitivity of the Eye" (PDF). Thulescientific.com. Retrieved 29 August 2017.. ...
A tutorial on analysis of ongoing, evoked, and induced neuronal activity: Power spectra, wavelet analysis, and coherence ... Frohlich, Joel; Senturk, Damla; Saravanapandian, Vidya; Golshani, Peyman; Reiter, Lawrence; Sankar, Raman; Thibert, Ronald; ... The spectrum of these SPs overlaps the gamma-band (see Gamma wave), and seriously confounds analysis of induced gamma-band ... Yang, H.; Ang, K.K.; Wang, C.; Phua, K.S.; Guan, C. (2016), "Neural and cortical analysis of swallowing and detection of motor ...
A detailed analysis of this spectrum is typically used to determine the identity and quantity of gamma emitters present in a ... Coherent anti-Stokes Raman spectroscopy. *Cold vapour atomic fluorescence spectroscopy. *Conversion electron mössbauer ... An example of a NaI spectrum is the gamma spectrum of the caesium isotope 137. Cs. -see Figure 1. 137. Cs. emits a single gamma ... If many gamma rays are present in a spectrum, Compton distributions can present analysis challenges. To reduce gamma rays, an ...
Some of the key uses for CT scanning have been flaw detection, failure analysis, metrology, assembly analysis, image-based ... Van de Casteele E, Van Dyck D, Sijbers J, Raman E (2004). "A model-based correction method for beam hardening artefacts in X- ... It occurs because conventional sources, like X-ray tubes emit a polychromatic spectrum. Photons of higher photon energy levels ... Wiener RS, Gould MK, Woloshin S, Schwartz LM, Clark JA (2012). ""What do you mean, a spot?": A qualitative analysis of patients ...
... "erythemal action spectrum".[50] The action spectrum shows that UVA does not cause immediate reaction, but rather UV begins to ... fluorescence of compounds in oil or by absorbing of UV created by Raman scattering in water.[90] ... These are often the emitting sources in UV spectroscopy equipment for chemical analysis. ... The long end of the EUV spectrum is set by a prominent He+ spectral line at 30.4 nm. EUV is strongly absorbed by most known ...
For his analyses of the fundamental limitations on the resolution in television systems and on wideband amplifiers, and for his ... For his outstanding technical contributions in the expansion of the useful spectrum of radio frequencies, and for the ... For fundamental contributions to quantum electronics, including the carbon dioxide laser and the spin-flip Raman laser.» ...
Vincent van Gogh, 'Wheatfield with Cypresses, 1889, pigment analysis at ColourLex *^ Bouchez, Colette. "The Lowdown on Mineral ... "Critical Wavelength & Broad Spectrum UV Protection". mycpss.com. Retrieved 15 April 2018.. ... "Anharmonic effects in ZnO optical phonons probed by Raman spectroscopy". Applied Physics Letters. 96 (15): 152103. Bibcode: ... The coating lets the visible part of the spectrum in but either reflects the infrared (IR) radiation back into the room (energy ...
Analysis of mass spectra can also be spectra with accurate mass. A mass-to-charge ratio value (m/z) with only integer precision ... Main article: Mass spectrum analysis. Since the precise structure or peptide sequence of a molecule is deciphered through the ... A mass spectrum is a plot of the ion signal as a function of the mass-to-charge ratio. These spectra are used to determine the ... Data analysis[edit]. Mass spectrometry data analysis is specific to the type of experiment producing the data. General ...
The International Forum on Globalization (IFG) is a San Francisco-based transnational alternative policy group that analyses ... convening smaller strategic seminars of movement leadership from a wide spectrum to discuss and design political campaigns, and ... Meenakshi Raman (co-chair). *Jack Santa Barbara. *Vandana Shiva. *Victoria Tauli-Corpuz ...
Stokes on the early history of spectrum analysis". Nature. 13: 188-189. doi:10.1038/013188c0.. ... The offshoot of this, Stokes line, is the basis of Raman scattering. In 1883, during a lecture at the Royal Institution, Lord ... Chemical analysis[edit]. The chemical identification of organic bodies by their optical properties was treated in 1864;[27] and ... A paper on the long spectrum of the electric light bears the same date,[25] and was followed by an inquiry into the absorption ...
The infrared spectrum typically has very narrow absorption lines, which makes them unsuited for quantitative analysis but gives ... Raman spectroscopy. *Photodetector - A transducer capable of accepting an optical signal and producing an electrical signal ... The analysis of the wavelength dependence is generally not done using a monochromator as it is in UV-Vis, but with the use of ... In airborne and space-based remote sensing such photon counters are used at the upper reaches of the electromagnetic spectrum ...
Raman spectroscopy proved that SLG had grown on nickel substrate. The SLG Raman spectrum featured no D and D′ band, indicating ... Grodecki, K.; Jozwik, I.; Baranowski, J. M.; Teklinska, D.; Strupinski, W. (2016). "SEM and Raman analysis of graphene on SiC( ... Graphene has three main features in its Raman spectrum, called the D, G, and 2D (also called G') modes that appear at about ... Raman spectroscopy and microscopy[edit]. Raman spectroscopy can provide information about the number of layers on graphene ...
Without the filters, the pigments themselves range only a small segment of the visual spectrum, about 490 to 550 nm.[24] Rows 5 ... T. York; S. Powell; S. Gao; L. Kahan; T. Charanya; D. Saha; N. Roberts; T. Cronin; J. Marshall; S. Achilefu; S. Lake; B. Raman ... Cronin, Thomas W.; Marshall, Justin (2001). "Parallel processing and image analysis in the eyes of mantis shrimps". The ... 12 for colour analysis in the different wavelengths (including six which are sensitive to ultraviolet light[21][25]) and four ...
Coherent anti-Stokes Raman spectrum of microscopy oil. Pump beam: 800 nm; Stokes beam: broadband from 1000 nm to 1100 nm;. anti ... Raman scattering. *Raman spectroscopy. *Instrumental analysis. *Scattering, absorption and radiative transfer (optics) ... Comparison to Raman spectroscopy[edit]. CARS is often compared to Raman spectroscopy as both techniques probe the same Raman ... To compare the spectra from multi-component compounds, the (resonant) CARS spectral amplitude should be compared to the Raman ...
Among the first to study quantum phenomena in nature were Arthur Compton, C. V. Raman, and Pieter Zeeman, each of whom has a ... "The essential lesson of the analysis of measurements in quantum theory is thus the emphasis on the necessity, in the account of ... If the operator's spectrum is discrete, the observable can attain only those discrete eigenvalues. ... especially the differences in the spectra of light emitted by different isotopes of the same chemical element, as well as ...
However, such solutions do show spectra of the hydrogen sulfite ion, HSO3−, by reaction with water, and it is in fact the ... Sulfur dioxide is fairly soluble in water, and by both IR and Raman spectroscopy; the hypothetical sulfurous acid, H2SO3, is ... Process screening analysis of alternative gas treating and sulfur removal for gasification. (December 2002) Report by SFA ...
Edwards, H. G. M.; Chalmers, G. M (2005). Raman spectroscopy in archaeology and art history. Royal Society of Chemistry. pp. ... Fujimoto, A.; Yamada, Y.; Koinuma, M.; Sato, S. (2016). "Origins of sp3C peaks in C1s X-ray Photoelectron Spectra of Carbon ... The most time-consuming part of the cutting is the preliminary analysis of the rough stone. It needs to address a large number ... Zaitsev, A. M. (2000). "Vibronic spectra of impurity-related optical centers in diamond". Physical Review B. 61 (19): 12909. ...
IEEE Spectrum: Elektrisidad Higit sa Glass . IEEE Spectrum. Oktubre 2005 *↑ Photovoltaic feat advances power over optical fiber ... Smith, R. G. (1972). "Optical Power Handling Capacity of Low Loss Optical Fibers as Determined by Stimulated Raman and ... mula sa electromagnetic analysis (tingnan sa ibaba). Sa isang step-index na hibla ng multi-mode, ang mga sinag ng liwanag ay ... "Origin of 'fiber fuse' is revealed". Photonics Spectra.. *↑ Seo; et al. "Evaluation of high-power endurance in optical fiber ...
Workman, Jerome; Workman, Jerry (2001). Handbook of organic compounds: NIR, IR, Raman, and UV-Vis spectra featuring polymers ... Dynamic mechanical analysis or DMA measures this complex modulus by oscillating the load and measuring the resulting strain as ... Iakovlev, V.; Guelcher, S.; Bendavid, R. (August 28, 2015). "Degradation of polypropylene in vivo: A microscopic analysis of ... Spectroscopy techniques, including Fourier-transform infrared spectroscopy, Raman spectroscopy, and nuclear magnetic resonance ...
Rotational Raman systems can take advantage of the temperature-dependent intensity of the rotational Raman band of laser light ... Objective methods consist of various forms of derivative methods,[43] wavelet analysis methods,[45] the variance method,[46] ... Temperature profiles at higher altitudes, up to 120 km, can be derived by measuring the broadening of absorption spectra of ... the Raman lidar detects the scattering of the laser beam due to Raman scattering. The frequency shift induced by such ...
Home » Theoretical analysis of the resonance Raman spectra of diacetylene single crystals ... We are able to describe the Raman excitation profiles as well as the Raman band profiles for two Raman active modes, i.e., the ... Resonance Raman spectra of polymer chains in the single crystals of the diacetylenes FBS (2,4-hexadiynylene-di-p-fluorobenzene ... The polarized reflection spectra of single crystals of PDA-CPDO [poly-1-(N-carbazolyl)penta-1, 3-diyn-5-ol], which are Ï€ ...
For the analysis of complex sensor signals, there is a need for improved algorithms and open platforms that enable techniques ... Second, an image guidance system was made for use with a sensor-integrated robotic system (a Raman probe attached to a surgical ... First, a software system was created to process, analyze, and classify data from a specific kind of sensor (a Raman ... system). The system supports tool tracking, sensor activation, real-time sensor data analysis, and presentation of the results ...
Analysis of the resonant raman spectra of viologens and of their radical cations using range-separated hybrid density ... Romanova, J, Liégeois, V and Champagne, B (2014) Analysis of the resonant raman spectra of viologens and of their radical ... In the case of the resonant Raman spectra, a μ value of 0.08-0.13 bohr-1 is adequate to reproduce the experimental spectrum of ... The UV/vis absorption spectra and resonance Raman spectra of methyl viologens and p-phenylene-extended viologens have been ...
To fully utilize Raman proling for single-cell analysis, an extensive understanding of Raman spectra is necessary to answer ... what strains can be distinguished by Raman spectra, and what features serve best as Raman-based biomarkers for single-cells, ... the classication performance of discretized spectra was found to be comparable to full spectrum having more than 1000 Raman ... In this work, we have proposed an approach called rDisc to discretize the original Raman spectrum into only a few (usually less ...
Vibrational Analysis of the Retinal Isomers (B. Curry, et al.). A Unified View of Raman, Resonance Raman and Fluorescence ... The Resonance Raman Effect and Depolarisation in Vibrational Raman Scattering (H. Hamaguchi). Low Frequency Depolarised Light ... Spectroscopy (and Their Analogues in Two-photon Absorption) (D. Lee and A. C. Albrecht). Magnetic Raman Optical Activity (L. D ... radiation Raman band Raman intensity Raman optical activity Raman scattering Raman spectra Raman Spectroscopy Raman tensor ...
raman spectra analysis software is a purely dedicated for Raman spectroscopy.During analysis period, get highest accurate and ... Data Analysis and processing. raman spectra analysis software is a purely dedicated for Raman spectroscopy. Its architect raman ... reference spectra (DCLS). • Domain size and distribution analysis from Raman images ... A Capacity of analysis options for single spectra:. • Identification of spectral (choose from an extensive range of databases ...
They combine Raman microscopy with scanning electron microscopy (SEM) to study inorganic and mineral samples. ... Raman spectrum and EDS analysis of pyrite. In both the Raman spectrum and EDS analysis, the metallic mineral content is ... Raman spectrum and EDS analysis of pyrite In both the Raman spectrum and EDS analysis, the metallic mineral content is ... In both the Raman spectrum and EDS analysis on the right, the metallic mineral content is identified as pyrite (iron sulphide, ...
... measuring intensities of Raman scattering from the plasma at frequencies in the frequency shift range between 1000 cm-1 and ... 1600 cm-1, and comparing the intensities to intensities of Raman scattering, at comparable frequencies, from normal human ... Method and device for chemical component spectrum analysis. US20080076983 *. Sep 27, 2006. Mar 27, 2008. Nellcor Puritan ... Raman/Resonance Raman Spectra. The Raman/resonance Raman spectrum is recorded using conventional Raman spectroscopic techniques ...
Multivariate Analysis * Spectrum Analysis, Raman / methods* * Statistics, Nonparametric * United States * Up-Regulation / ... Here, we demonstrate the potential of Raman spectroscopic analysis of wound biopsies for classification of wounds as normal or ... Monitoring the healing of combat wounds using Raman spectroscopic mapping Wound Repair Regen. Jul-Aug 2010;18(4):409-16. doi: ... Raman spectroscopy showed a loss of collagen in five patients, indicated by a negative percent difference in the 1,665/1,445 cm ...
NORMAL-MODE ANALYSIS; RAMAN SPECTRA; RAMAN SPECTROSCOPY; RESONANCE; SELF-CONSISTENT FIELD; SOLVENTS; WAVELENGTHS. ... and the normal mode analysis. The A-, B-, and C-bands resonance Raman spectra in cyclohexane, acetonitrile, and methanol ... The vibrational assignments were done on the basis of the Fourier transform (FT)-Raman and FT-infrared measurements, the ... Title: Structural dynamics of phenylisothiocyanate in the light-absorbing excited states: Resonance Raman and complete active ...
Raman and IR spectroscopy studies on propane were performed at pressures of up to 40 GPa at ambient temperatures using the ... Spectrum Analysis, Raman. Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of ... However, the Raman spectrum of a bacte.... Predicting post-mortem meat quality in porcine longissimus lumborum using Raman, ... The effect of pressure on the Raman and fluorescence spectra of hematin anhydride (β-hematin) is reported. In a diamond-anvil ...
The goal of this research is to determine whether Raman spectroscopy (RS), an optical method that probes the vibrational modes ... Spectrum Analysis, Raman*. Grant Support. ID/Acronym/Agency: HD 044741/HD/NICHD NIH HHS; R01 HD044741/HD/NICHD NIH HHS; R01-CA- ... Raman spectra were acquired before, during and after a 19-day mouse gestational period. In some cases, after Raman data was ... Various peaks of the Raman spectra, such as the areas corresponding to fatty acid content and collagen organization, changed as ...
Calculation of Raman optical activity spectra for vibrational analysis Shaun T. Mutter, François Zielinski, Paul L. A. Popelier ... Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy ... Development of Transmission Raman Spectroscopy towards the in line, high throughput and non-destructive quantitative analysis ... Transmission Raman Spectroscopy and photon diode enhancer facilitates faster scanning and quantitative analysis of ...
... time-resolved absorption spectra and transient absorption time-resolved spectral images can be measured simultaneously, and you ... The time-resolved absorption spectrum analysis system is a device to perform transient absorption spectrum measurements in ... Time-resolved absorption spectrum analysis system has been saved to your wishlist successfully. ... Fluorescence lifetime / transient absorption analysis system. Picosecond fluorescence lifetime measurement system. Compact NIR ...
Time-resolved absorption spectrum analysis system has been saved to your wishlist successfully. ... Fluorescence lifetime / transient absorption analysis system. Quantaurus-Tau Fluorescence lifetime spectrometer. Picosecond ...
Raman presents a non-destructive form of sample analysis and thus the samples can be further used for a method validation. We ... Based on its relevance, the analysis of such kinds of cells is within the scope of many research and clinical institutes; ... Here we used a state-of-the-art micro-Raman microscopic technique to enhance possibilities in the study of circulating tumor ... The acquired data were visualized using both chemigrams and hierarchical clustering analysis. ...
Acquisition, preprocessing and analysis of Raman spectra. Sergey Kucheryavskiy (Speaker). 31 May 2013 ... Analysis of hyper spectral images using spectral features calculated for blocks of pixels. Sergey Kucheryavskiy (Speaker) ...
Analysis and characterisation of Raman spectra.. Smith, SL, Lones, MA, Bedder, M, Alty, JE, Cosgrove, J, Maguire, RJ, Pownall, ... Prins, D, Plank, T, Baseler, HA, Gouws, AD, Beer, A, Morland, AB, Greenlee, MW & Cornelissen, FW 2016, Surface-Based Analyses ... Halliday, DM, Senik, MH, Stevenson, CW & Mason, R 2016, Non parametric directionality analysis - extension for removal of a ...
Infrared, Raman and temperature-dependent NMR spectra, vibrational assignments, normal coordinate analysis, and DFT ... Raman and temperature-dependent NMR spectra, vibrational assignments, normal coordinate analysis, and DFT calculations of ...
Spectrum Analysis, Raman • Strepsirhini • Temperature • Testis • Thermometers • Tiletamine • Time Factors • Tooth • Tooth ... Adaptation, Biological • Age Factors • Aging • Algorithms • Alouatta • Analysis of Variance • Anesthetics • Animal Feed • ... spectrum, timing) during the day and during the night. A collaboration with Rennselaers Lighting Research Center (LRC) was ... Least-Squares Analysis • Leg • Lemur • Lemurs • Lipids • Lipoproteins • Lipoproteins, HDL • Lipoproteins, LDL • Lipoproteins, ...
Raman spectra analysis confirms these findings. Increase in CNT defectiveness indicates formation of C-C bonds between ... Using the analysis of Raman spectra [89,90,91], the chirality indexes (x,y) were determined for SWCNT: (8,4); (16,0); (10,6); ( ... Figure 7. Raman spectra of (a,b) CNT and (c,d) CNT/rGO hybrids before (black line) and after (red line) laser irradiation with ... Figure 7. Raman spectra of (a,b) CNT and (c,d) CNT/rGO hybrids before (black line) and after (red line) laser irradiation with ...
... we have developed a Raman spectroscopy based molecular imaging method and combined multivariate curve resolution analysis to ... Raman spectrum representative of each intracellular location is shown in Fig. 1. Major features in lipid Raman spectrum (Fig. ... Results from MCR analysis are highly desirable as it separates pure chemical spectra that are meaningful. Though Raman imaging ... In order to understand molecular origin of components 3 and 4 from MCR analysis, Raman spectra from a series of potential ...
Multivariate analysis of Raman spectra has enabled development of a quantitative measure for wound healing progress.[19] ... The advanced types of Raman spectroscopy include surface-enhanced Raman, resonance Raman, tip-enhanced Raman, polarized Raman, ... and λ1 is the Raman spectrum wavelength. Most commonly, the unit chosen for expressing wavenumber in Raman spectra is inverse ... By using Raman microspectroscopy, in vivo time- and space-resolved Raman spectra of microscopic regions of samples can be ...
Spectrum Analysis, Raman. Grant Support. ID/Acronym/Agency: DK 53053/DK/NIDDK NIH HHS ...
Spectrum Analysis, Raman; X-Ray Diffraction. ... micrograph analysis, the particle size ranges from a few ... Electron paramagnetic resonance (EPR) spectrum exhibits two resonance signals at magnetic fields of 2895 and 3106 gauss with g- ...
Spectrum Analysis, Raman. Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of ... Spatial Risk Analysis of Power Systems Resilience During Extreme Events.. The increased frequency of extreme events in recent ... Joint probabilistic analysis of risk for aquatic species and exceedence frequency for the agricultural use of chlorpyrifos in ... Resonance Frequency Analysis. A non-invasive assessment of the stability of tissue-embedded prosthetic devices such as dental ...
Anharmonic vibrational analysis for VCD and ROA spectra: see Freq=Anharmonic. *Vibronic spectra and intensities: see Freq=FCHT ... TD-DFT analytic second derivatives for predicting vibrational frequencies/IR and Raman spectra and performing transition state ...
Feature visualization of Raman spectrum analysis with deep convolutional neural network.. Fukuhara M, Fujiwara K, Maruyama Y, ... Analysis of key clinical features for achieving complete remission in stage III and IV non-small cell lung cancer patients. ... Comparative genome-wide analysis of gastric adenocarcinomas with hyperplastic polyp components.. Takayama Y, Ono Y, Mizukami Y ... Cost-Effectiveness of Cardiac Rehabilitation in Patients with Coronary Artery Disease: A Meta-Analysis. ...
Raman spectra analysis exhibits several bands characteristic of cellulose... The weaker presence of lignin is also attested to ... Spectra from modern Quercus and Juniperus are included as examples of hardwood and softwood respectively. Note that the peaks ... 5. Analyses focused outside the cord sample did not exhibit an organic substance signature. These results confirm that the ... by the band at 1602 cm-1. Several micro-analyses were performed at different locations on the cord sample and yielded the same ...
  • Please visit www.renishaw.com/earthsciences for further details on how Raman microscopy is being used in earth sciences applications. (renishaw.com)
  • From the scanning electron microscopy (SEM) micrograph analysis, the particle size ranges from a few micrometres to 5 μm. (americanelements.com)
  • Each group is examined for biochemical changes using Raman microscopy, cell viability changes using confocal fluorescence microscopy, and histological changes using the modified Mankin score. (spiedigitallibrary.org)
  • We analyzed the nanodiamond-derived onion-like carbon (OLC) as function of synthesis temperature (1000~1400 °C), by high-resolution electron microscopy, electron energy loss spectroscopy, visible-Raman spectroscopy, ultraviolet photoemission spectroscopy, impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry. (nature.com)
  • Raman spectrometry uses Raman spectroscopy, chemical analysis by Spectral Laser Spectrum Collapse (LIBS), and Surface Analysis by SEM (scanning electron microscopy). (thefreedictionary.com)
  • Tip-enhanced Raman spectroscopy, or TERS, integrates scanning probe microscopy (SPM) for nano-scale imaging with confocal Raman spectroscopy for chemical characterization. (horiba.com)
  • The structure of the prepared samples was studied by X-ray diffraction (XRD) and Raman spectroscopy and the morphology by scanning electron microscopy (SEM). (intechopen.com)
  • The Resonance Raman Effect and Depolarisation in Vibrational Raman Scattering (H. Hamaguchi). (google.com)
  • A method for determining the presence of malignancy disease in a subject person includes obtaining a test sample of blood plasma from the person, measuring intensities of Raman scattering from the plasma at frequencies in the frequency shift range between 1000 cm-1 and 1600 cm-1, and comparing the intensities. (google.com)
  • A method for determining the presence of malignancy disease in a subject person includes obtaining a test sample of blood plasma from the person, measuring intensities of Raman scattering from the plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , and comparing the intensities to intensities of Raman scattering, at comparable frequencies, from normal human plasma. (google.com)
  • 8. The method of claim 3, 5, 6, or 7 wherein said intensities of Raman scattering are recorded at regular intervals over at least a portion of said frequency shift range. (google.com)
  • Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light. (bioportfolio.com)
  • It relies on inelastic scattering , or Raman scattering , of monochromatic light, usually from a laser in the visible , near infrared , or near ultraviolet range. (wikipedia.org)
  • Spontaneous Raman scattering is typically very weak, and as a result the main difficulty of Raman spectroscopy is separating the weak inelastically scattered light from the intense Rayleigh scattered laser light. (wikipedia.org)
  • The intensity of the Raman scattering is proportional to this polarizability change. (wikipedia.org)
  • Therefore, the Raman spectrum (scattering intensity as a function of the frequency shifts) depends on the rovibronic states of the molecule. (wikipedia.org)
  • Raman scattering also contrasts with infrared (IR) absorption, where the energy of the absorbed photon matches the difference in energy between the initial and final rovibronic states. (wikipedia.org)
  • The plasmonic properties of the obtained colloids, as well as their adsorption capability, were tested by surface-enhanced Raman scattering (SERS) spectroscopy using 2,2′-bipyridine as a probe molecule. (mdpi.com)
  • Nanoparticles constituted of metals like silver, gold, or copper exhibit plasmonic properties and are widely employed as biosensors, drug vectors, and SERS (surface-enhanced Raman scattering) [ 1 , 2 ] and fluorescence markers, especially gold nanoparticles that are more biocompatible. (mdpi.com)
  • Raman spectroscopy is a spectroscopic technique that detects photons that have undergone Raman scattering. (thorlabs.com)
  • Since Raman scattering is relatively weak compared to Rayleigh scattering, one of the main historical problems with Raman spectroscopy had been separating out the weak Raman signal from the strong Rayleigh signal. (thorlabs.com)
  • Raman scattering is a weak, low-probability event with a 1/λ 4 wavelength-dependent efficiency. (thorlabs.com)
  • When using a laser to produce Raman scattering in a sample, sufficient power is important to improve the sensitivity and integration time of measurements. (thorlabs.com)
  • The intensity of Raman scattering from dye molecules strongly coupled with localized surface plasmons of metal nanostructures was controlled by the electrochemical potential. (rsc.org)
  • Through in situ electrochemical extinction and surface-enhanced Raman scattering measurements, it is found that the redox state of the molecules affects the coupling strength, leading to the change in the intensity of the Raman scattering. (rsc.org)
  • Analysis of the Raman spectrum provides information on the molecules in strong coupling states showing effective enhancement of Raman scattering. (rsc.org)
  • Raman scattering or the Raman effect ( / ˈ r ɑː m ən / ) is the inelastic scattering of photons by matter, meaning that there is both an exchange of energy and a change in the light's direction. (wikipedia.org)
  • This is called normal Stokes Raman scattering. (wikipedia.org)
  • The inelastic scattering of light was predicted by Adolf Smekal in 1923 [3] and in older German-language literature it has been referred to as the Smekal-Raman-Effekt. (wikipedia.org)
  • Raman received the Nobel Prize in 1930 for his work on the scattering of light. (wikipedia.org)
  • [10] Early spectra took hours or even days to acquire due to weak light sources, poor sensitivity of the detectors and the weak Raman scattering cross-sections of most materials. (wikipedia.org)
  • The following focuses on the theory of normal (non-resonant, spontaneous, vibrational) Raman scattering of light by discrete molecules. (wikipedia.org)
  • Raman scattering generally gives information about vibrations within a molecule. (wikipedia.org)
  • Raman spectroscopy is an inelastic scattering process capable of providing information about biochemical and biomolecular structures and conformations of samples. (spiedigitallibrary.org)
  • Raman scattering occurs when light interacts with a molecular vibration/rotation and a change in polarizability takes place during molecular motion. (springer.com)
  • Fluorescence and Raman scattering are examples of inelastic processes. (springer.com)
  • Surface Enhanced Raman Scattering (SERS) can detect the pathogen in rapid and accurate. (spie.org)
  • In SERS weak Raman scattering signals are enhanced by many orders of magnitude. (spie.org)
  • Influence of the solvent environment on the polarization properties of resonance Raman scattering in hemoglobin. (drexel.edu)
  • 2) R. Schweitzer-Stenner, W. Dreybrodt and S el Naggar, Investigation of pH-induced Symmetry Distortions of the Prosthetic Group in Deoxyhemoglobin by Resonance Raman Scattering. (drexel.edu)
  • Determination of the Raman Tensor of the Haem Group in Myoglobin by Resonance Raman Scattering in Solution and Single Crystals. (drexel.edu)
  • Investigation of pH-induced symmetry distortions of the prosthetic group in oxyhaemoglobin by resonance Raman scattering. (drexel.edu)
  • Haem-apoprotein interactions detected by resonance Raman scattering in Mb- and Hb-derivatives lacking the saltbridge His 146b-Asp 94b. (drexel.edu)
  • Heme-apoprotein interaction in the modified oxyhemoglobin-bis-(N-maleimidomethyl)ether and in oxyhemoglobin at high Cl-concentration detected by resonance Raman scattering. (drexel.edu)
  • Detection of heme distortions in ferri- and ferrocytochrome c by resonance Raman scattering. (drexel.edu)
  • Correspondence of the pK-values of oxyHb-titration states detected by resonance Raman scattering to kinetic data of ligand dissociation and association. (drexel.edu)
  • T transition in oxyhemoglobin trout IV by resonance Raman scattering. (drexel.edu)
  • The influence of structural variations in the F- and FG-helix of the β -subunit modified oxyHb-NES on the heme structure detected by resonance Raman scattering. (drexel.edu)
  • Meanwhile, Surface-enhanced Raman scattering (SERS) has such advantages as high sensitivity, flexible excitation wavelength, high spectral resolution, non-invasiveness to biological samples, resistance self-fluorescence, photobleaching, etc., which is considered as a promising and powerful real-time detection technology for unlabeled cells. (clinicaltrials.gov)
  • The report reviews briefly several methods for interpreting neutron scattering data in terms of center frequency of infrared and Raman Spectra. (dtic.mil)
  • Raman spectroscopy can be used to identify molecules such as DNA by the characteristic scattering of light from a laser. (strath.ac.uk)
  • We evaluate the feasibility of applying polarized Raman spectroscopy in probing the early biochemical compositions and orientation changes in impacted porcine cartilage explants. (spiedigitallibrary.org)
  • 0.05, n = 5) reveal no significant changes when compared to the control, but polarized Raman spectroscopy detects significant biochemical changes. (spiedigitallibrary.org)
  • This suggests that the polarized Raman spectroscopy technique has potential for diagnosis and detection of early cartilage damage at the molecular level. (spiedigitallibrary.org)
  • A thermoelectrically cooled surface-enhanced Raman Spectroscopy (TEC-SERS) fiber optic probe for real-time and in-situ monitoring of volatile organic compounds in gas, liquid, and soil environments. (google.com)
  • The TEC-SERS probe comprises a sample chamber for receiving a gas sample and a fiber optic Raman probe. (google.com)
  • The fiber optic Raman probe is operably coupled to a transparent window in the sample chamber for directing an optical excitation signal to irradiate the SERS substrate and for receiving a SERS optical signal from analytes from the gas sample that are in contact with the SERS substrate. (google.com)
  • a fiber optic Raman probe operably coupled to said window in said sample chamber for directing an optical excitation signal to irradiate said SERS substrate and for receiving a SERS optical emissions signal. (google.com)
  • Among these methods vibrational spectroscopic techniques (IR, Raman and SERS) are useful tools because of their rapidity and sensitivity. (semanticscholar.org)
  • But SERS spectrum of cell contains information about different molecules, so it usually requires complex data interpretation. (clinicaltrials.gov)
  • First, a software system was created to process, analyze, and classify data from a specific kind of sensor (a Raman spectrometer). (wayne.edu)
  • The image below shows a Raman Spectrometer system constructed using Thorlabs components designed for 780 nm light, including a retired TLK-780M Tunable Laser Kit fed into a retired Tapered Amplifier. (thorlabs.com)
  • The cuvette holder allowed optical access to all four sides of the cuvette, making it ideal for a Raman spectrometer. (thorlabs.com)
  • The Raman spectra for isopropyl alcohol, measured with this 780 nm Raman spectrometer, is presented to the lower right. (thorlabs.com)
  • A Raman spectrometer constructed from Thorlabs components. (thorlabs.com)
  • Raman spectrum for isopropyl alcohol, measured with the 780 nm Raman spectrometer discussed above. (thorlabs.com)
  • The RAM II from Bruker is the first dual channel FT-Raman module that is capable of being coupled to the VERTEX FT-IR research spectrometer series. (azooptics.com)
  • The Compact Raman Analyzer (Cora) series consists of a handheld Raman spectrometer for on-the-spot identification, confirmation of substances in the field, or verification measurements on incoming goods, as well as a range of benchtop Raman spectrometers for quality assurance, and for industrial and academic research. (anton-paar.com)
  • Are you currently using a Raman spectrometer? (anton-paar.com)
  • Cora 5001 is Anton Paar's Raman spectrometer for quickly identifying substances, even through packaging. (anton-paar.com)
  • The high spectral resolution of a Raman spectrometer can be useful in performing photoluminescence spectroscopy of solid-state materials, particularly when the emission spectra consist of narrow bands or even lines. (horiba.com)
  • When used to perform photoluminescence spectroscopy, the Raman spectrometer becomes two instruments in one. (horiba.com)
  • The excited state structural dynamics of phenyl isothiocyanate (PITC) after excitation to the light absorbing S{sub 2}(A′), S{sub 6}(A′), and S{sub 7}(A′) excited states were studied by using the resonance Raman spectroscopy and complete active space self-consistent field method calculations. (osti.gov)
  • The proposed research evaluates tissue oxygenation (StO2) as measured by resonance raman spectroscopy (RRS) in premature infants with and without patent ductus arteriosus (PDA). (bioportfolio.com)
  • Historically, Raman spectrometers used holographic gratings and multiple dispersion stages to achieve a high degree of laser rejection. (wikipedia.org)
  • Similarly, recording the Raman spectrum has been aided greatly by the advent of CCD spectrometers . (thorlabs.com)
  • Traditionally, many Raman spectrometers have been built around 785 nm chips. (thorlabs.com)
  • The second system (MOON 2) is a multi-band and multimodal functional imaging platform for in-depth diagnostics of ocular and neurodegenerative diseases on a molecular level combining the chemical specificity of Raman spectroscopy with the structural and functional sensitivity of OCT. For this task novel light source technology, advanced Raman spectrometers, and multimodal test eyes will be developed. (europa.eu)
  • We have considered the influence of fixation methods of the cells, where we found out that the ability of Raman spectroscopy to differentiate between three cell lines strictly depends upon the sample preparation method used. (nih.gov)
  • Chemometric analysis showed the ability of Raman spectroscopy to predict the functional class of an unknown antibiotic and to identify individual antibiotics that elicit similar phenotypic responses. (asm.org)
  • They use Renishaw's structural and chemical analyser (SCA) interface to bring the Raman analysis capabilities of the inVia™ confocal Raman microscope to their scanning electron microscope (SEM). (renishaw.com)
  • The inVia and SCA interface provide an in-SEM analytical technique that allows co-located chemical information and the flexibility of a fully functioning Raman microscope. (renishaw.com)
  • Small amount of these cells were loaded on the metal substrate individually, scanned and spectra were recorded using confocal Raman microscope. (spie.org)
  • A sample of 100 microcontainers of each size was selected and measured on a Raman microscope over a period of 30days to ascertain whether the indomethacin in each container was amorphous or crystalline. (dtu.dk)
  • The Raman spectra of a particular face of a single crystal can be significantly different if acquired with different microscope objectives. (horiba.com)
  • The purpose of this installment of "Molecular Spectroscopy Workbench" is to inform and educate users of micro-Raman instrumentation of the effect of the microscope objective on the Raman spectra of crystals. (horiba.com)
  • Furthermore, we explain the underlying physics of changes in relative intensity and even peak position of certain Raman bands depending on the microscope objective used to acquire the spectrum. (horiba.com)
  • Baraldi used three types of instruments that allow for a non-destructive approach: a digital microscope connected to a computer, instrumentation using a Wood's lamp with ultraviolet radiation, and a portable Raman microscope. (humanitiesebook.org)
  • Multivariate analysis was also used to classify adulterated milk powder samples. (ibict.br)
  • Experience with statistics and chemometric modeling using multivariate analysis such as PLS/SIMCA/PCA, etc. (astri.org)
  • The technique employs near infrared Raman laser excitation at 785 nm, multivariate analysis, non-linear artificial neural networks and an offset spectra subtraction strategy. (sae.org)
  • An even smaller fraction of the scattered photons (approximately 1 in 10 million) can be scattered inelastically , with the scattered photons having an energy different (usually lower) from those of the incident photons-these are Raman scattered photons. (wikipedia.org)
  • If matter is irradiated with light at a precisely defined frequency, some of the photons of this light interact with the molecules of the matter, which thereby shifts the photons' own energy spectrum. (eurekalert.org)
  • Label-free Raman characterization of bacteria calls for standardized procedures. (bioportfolio.com)
  • Our spectroscopic results reveal that RS analysis of total protein of cell line irradiated by X-ray, in conjunction with mathematical statistical model, can be a potential method to explore the Raman characterization target of cell radiosensitivity, and then for making known the mechanism of radiosensitivity of tumor further. (hindawi.com)
  • Since each sample has a unique composition, the spectroscopic profile arising from Raman-active functional groups of nucleic acids, proteins, lipids, and carbohydrates allows for the evaluation, characterization, and discrimination of tissue type. (springer.com)
  • Because the resonance Raman spectrum varies with subtle chemical changes on the functional side groups, the spectra of carotenoids have been used for identification and characterization. (horiba.com)
  • named after Indian physicist Sir C. V. Raman ) is a spectroscopic technique used to observe vibrational, rotational, and other low-frequency modes in a system. (wikipedia.org)
  • Ultra violet resonance Raman (UVRR) is a powerful spectroscopic technique for determining the secondary structural content of proteins in solution. (umsystem.edu)
  • [1] Raman spectroscopy is commonly used in chemistry to provide a structural fingerprint by which molecules can be identified. (wikipedia.org)
  • It contains a detailed analysis of the vibrational Raman spectra of the chain molecules, which is supported by comprehensive quantum chemical simulations. (springer.com)
  • Emission spectra obtained from these plasmas, contained spectral lines corresponding to radiative transitions in atoms, molecules, atomic or molecular ions. (spiedigitallibrary.org)
  • it senses the relative amount of light that molecules absorb due to their unique vibrational resonances falling within the MIR portion of the electromagnetic spectrum (i.e., wavelengths from approximately 2 to 15 µm). (photonics.com)
  • The use of vibrational spectroscopy, FTIR and Raman, for cytology and cellular research has the potential to revolutionise the approach to cellular analysis. (rsc.org)
  • The effect of pressure on the Raman and fluorescence spectra of hematin anhydride (β-hematin) is reported. (bioportfolio.com)
  • Anharmonicities derived from low-temperature fluorescence spectra (see 6 ) are approximately 8.7 Ikezawa and Rolfe, 1973 . (nist.gov)
  • Micro-Raman high-pressure investigation on the malaria pigment hematin anhydride (β-hematin). (bioportfolio.com)
  • Here we used a state-of-the-art micro-Raman microscopic technique to enhance possibilities in the study of circulating tumor cells and their further differentiation. (nih.gov)
  • Micro-Raman spectroscopic identification of bacterial cells of the genus Staphylococcus and dependence on their cultivation conditions. (semanticscholar.org)
  • article{Harz2005MicroRamanSI, title={Micro-Raman spectroscopic identification of bacterial cells of the genus Staphylococcus and dependence on their cultivation conditions. (semanticscholar.org)
  • Ms Barbu commented, "This allows us to perform high resolution analysis on geological samples using energy dispersive spectroscopy (EDS) and Raman spectroscopy. (renishaw.com)
  • In the past, photomultipliers were the detectors of choice for dispersive Raman setups, which resulted in long acquisition times. (wikipedia.org)
  • Schematic of a dispersive Raman spectroscopy setup in a 180° backscattering arrangement. (wikipedia.org)
  • Detecting biochemical changes in the rodent cervix during pregnancy using Raman spectroscopy. (biomedsearch.com)
  • Biochemical fractionation revealed that astrocytes may be converted into harmful cells by the inflammatory EV cargo, as indicated by immunohistochemical and qPCR analyses, whereas surface lipid components of EVs promote OPC migration and/or differentiation, linking EV lipids to myelin repair. (springer.com)
  • Recently, this technique has proved to be very useful within the biomedical imaging sector for label-free, biochemical analyses of cells, tissue and biofluids. (photonics.com)
  • The resulting Raman spectra contained sufficient biochemical information to distinguish between profiles induced by individual antibiotics belonging to the same class. (asm.org)
  • In this study, we have developed a Raman spectroscopy based molecular imaging method and combined multivariate curve resolution analysis to enable detection and visualization of multiple polysaccharide components simultaneously at the single cell level. (nature.com)
  • In addition, applying laser radiation allowed online detection of the Raman spectra providing thereby the capabilities to monitor the temperature, partial volume, and nanocrystal sizes in nc-Si composite during its formation and showing the possibility to control efficiently the crystallization process by varying the intensity and irradiation time of laser light. (hindawi.com)
  • A compilation of papers showcasing the latest discoveries and developments in the analysis and detection of food fraud, contamination, adulteration and spoilage. (rsc.org)
  • Hepcidin Detection by Affinity Based Sensing: a Possible Application in Clinical and Anti-Doping Analysis. (powells.com)
  • This review provides an overview of the theory of Raman spectroscopy, instrumentation used for measurement, and variation of Raman spectroscopic techniques for clinical applications in cancer, including detection of brain, ovarian, breast, prostate, and pancreatic cancers and circulating tumor cells. (springer.com)
  • The phase transitions were identified using the Raman and IR splitting modes and the appearance or disappearance of peaks, which clearly corresponded to the changes in the frequencies of the modes as the pressure changed. (bioportfolio.com)
  • TD-DFT analytic second derivatives for predicting vibrational frequencies/IR and Raman spectra and performing transition state optimizations and IRC calculations for excited states. (gaussian.com)
  • Vibrational frequencies and absorption and Raman spectra. (elsevier.com)
  • Find important wavelengths/frequencies in the spectra. (originlab.com)
  • The Raman effect is based on the interaction between the electron cloud of a sample and the external electrical field of the monochromatic light, which can create an induced dipole moment within the molecule based on its polarizability. (wikipedia.org)
  • Electron paramagnetic resonance (EPR) spectrum exhibits two resonance signals at magnetic fields of 2895 and 3106 gauss with g-values of 2.32 and 2.16, respectively, calculated using Lande's formula. (americanelements.com)
  • We then used high-performance liquid chromatography and Raman spectroscopy to chemically assess the family-level distribution of plumage carotenoids, confirming their presence in 95 of 236 extant bird families (only 36 family-level occurrences had been confirmed previously). (royalsocietypublishing.org)
  • Vibronic spectra and intensities: see Freq=FCHT and related options. (gaussian.com)
  • The vibrational assignments were done on the basis of the Fourier transform (FT)-Raman and FT-infrared measurements, the density-functional theory computations, and the normal mode analysis. (osti.gov)
  • Power is important in Raman measurements. (thorlabs.com)
  • Both Raman and infrared (IR) spectroscopy probe molecular vibrations associated with chemical bonds in a sample to obtain information on molecular structure, composition, and intermolecular interactions. (springer.com)
  • Molecular Spectra and Molecular Structure. (barnesandnoble.com)
  • During the second reporting period the key milestone achievement was to demonstrate, that Raman spectroscopy can be applied to the human eye, given the constraints on laser power as applied to the eye, as well as the measurement duration, that should not exceed 30seconds. (europa.eu)
  • The RAM II provides the ideal FT-Raman measurement. (azooptics.com)
  • We used SilviScan analysis and tracheid measurement to evaluate the effect of suppressed growth on the fundamental properties of wood fiber. (usda.gov)
  • On-line measurement of fat, water, protein, colour and colour defects by NIR andVIS spectroscopy and imaging · Determination of connective tissue in meat and fish by fluorescence spectroscopy · Raman spectroscopy on biomaterials - how to cope with background fluorescence At our institute I am a principal researcher and project manager for one of ten basic research projects: Effective assessment of food quality by rapid spectroscopic and sensor techniques (Strategic institute project). (nofima.no)
  • Cell biologists at IGB use a specially developed Raman spectroscope jointly designed and built with physicists at the Fraunhofer Institute for Physical Measurement Techniques IPM in Freiburg, Germany. (eurekalert.org)
  • The advanced types of Raman spectroscopy include surface-enhanced Raman , resonance Raman , tip-enhanced Raman , polarized Raman, stimulated Raman (analogous to stimulated emission ), transmission Raman, spatially offset Raman, and hyper Raman . (wikipedia.org)
  • [2] The Raman effect should not be confused with emission ( fluorescence or phosphorescence ), where a molecule in an excited electronic state emits a photon and returns to the ground electronic state, in many cases to a vibrationally excited state on the ground electronic state potential energy surface. (wikipedia.org)
  • For some applications, other wavelengths may be desired to balance the 1/λ 4 Raman signal dependence with the background fluorescence of a particular sample. (thorlabs.com)
  • Second, an image guidance system was made for use with a sensor-integrated robotic system (a Raman probe attached to a surgical system). (wayne.edu)
  • To develop and endoscopic Raman spectroscopy probe for delivery down and channel in an endoscope to make near instant assessments of the condition of the oesophagus without the need for ex. (bioportfolio.com)
  • IR spectroscopy and Raman spectroscopy are complementary techniques that differ in their methodology to probe vibration. (springer.com)
  • Rapid diagnosis and differentiation of microbial pathogens in otitis media with a combined Raman spectroscopy and low-coherence interferometry probe: toward in vivo implementation. (semanticscholar.org)
  • This work describes tin-induced crystallization of amorphous silicon studied with Raman spectroscopy in thin-film structures Si-Sn-Si irradiated with pulsed laser light. (hindawi.com)
  • The crystallisation numbers from the microcontainers were compared with the crystallisation kinetics of the amorphous bulk indomethacin, as determined by FT-Raman spectroscopy.Comparison of the numeric crystallisation in the microcontainers with the crystallisation kinetics of the amorphous bulk indomethacin showed that spatial confinement of indomethacin led to a significantly lower extent of crystallisation of the amorphous form. (dtu.dk)
  • Why Are the Raman Spectra of Crystalline and Amorphous Solids Different? (horiba.com)
  • The Raman spectra of crystalline and amorphous solids of the same chemical composition can be significantly different primarily because of the presence or absence of spatial order and longrange translational symmetry, respectively. (horiba.com)
  • Analysis of the Raman spectra demonstrates that the carbon is similar to amorphous carbons. (unl.edu)
  • In this research, E. coli cultures were subjected to three times the MICs of 15 different antibiotics (representing five functional antibiotic classes) with known mechanisms of action for 30 min before being analyzed by Raman spectroscopy (using a 532-nm excitation wavelength). (asm.org)
  • Were it not for the problem of photoluminescence, only one laser excitation wavelength would be necessary to perform Raman spectroscopy. (horiba.com)
  • The magnitude of the Raman effect correlates with polarizability of the electrons in a molecule. (wikipedia.org)
  • For a molecule to exhibit a Raman effect, there must be a change in its electric dipole-electric dipole polarizability with respect to the vibrational coordinate corresponding to the rovibronic state. (wikipedia.org)
  • IR spectroscopy monitors the net absorption of incident radiation by a sample in the IR region of the electromagnetic spectrum (and depends on a net change in dipole moment of a molecule as it vibrates/rotates). (springer.com)
  • Diatomic Molecule Spectra and Energy Levels Data Center. (barnesandnoble.com)
  • These systems provide correlated nano-scale Raman imaging, enabling scientists to visualize, chemically characterize and implement change, even at the single-molecule level. (horiba.com)
  • Lipoprotein analysis and the utilization of physical-analytical techniques are likewise provided. (elsevier.com)
  • The work shows the Raman spectroscopy as an analytical tool that can be used in quality control of milk powder and condensed milk. (ibict.br)
  • Resonance Raman spectra of polymer chains in the single crystals of the diacetylenes FBS (2,4-hexadiynylene-di-p-fluorobenzene sulfonate), TS/FBS [6-(p-toluenesulfonyloxy)-2,4-p-fluorobenzene sulfonate] and TS6 (2,4-hexadiynylene-di-p-toluene sulfonate) are described theoretically by means of a Franckâ€"Condon model, which considers a chain length dependence. (ebscohost.com)
  • The earlier papers of the team presented the results of theoretical analyses concerning the interaction of CW laser radiation with metal targets. (spiedigitallibrary.org)
  • [4] In 1922, Indian physicist C. V. Raman published his work on the "Molecular Diffraction of Light", the first of a series of investigations with his collaborators that ultimately led to his discovery (on 28 February 1928) of the radiation effect that bears his name. (wikipedia.org)
  • Consider purchasing books including Molecular Spectroscopy, Organic Structural Spectroscopy, Spectrochemical Analysis and Powder Diffraction: Theory and Practice to name just a few. (valorebooks.com)
  • We have recently started applying a new approach to lignin analysis - namely using nano- and micro-particles of silver for study of native and residual. (usda.gov)
  • Neste trabalho, a espectroscopia FT-Raman foi explorada como técnica rápida para avaliação da qualidade do leite em pó e leite condensado. (ibict.br)
  • A análise exploratória empregando os dados da espectroscopia Raman e a análise por componentes principais (PCA) permitiu a separação das amostras de leite em pó. (ibict.br)
  • As modificações que ocorrem no leite em pó com o tempo de armazenamento foram acompanhadas pela espectroscopia Raman, as mudanças observadas nos espectros indicam a cristalização da lactose como a principal alteração do leite armazenado. (ibict.br)
  • O trabalho desenvolvido mostra a espectroscopia Raman como uma ferramenta analítica que pode ser utilizada no controle de qualidade do leite em pó e de leite condensado. (ibict.br)
  • A significant decrease in the parallel polarized intensity of the pyranose ring band at 1126 cm −1 suggests a possible decrease in the glycoaminoglycan content in early cartilage damage (one-way analysis of variance with a post hoc Bonferonni test, p (spiedigitallibrary.org)
  • New instrumentation is addressing these challenges, allowing TERS to migrate from pure research to routine laboratory analysis. (horiba.com)
  • Joint probabilistic analysis of risk for aquatic species and exceedence frequency for the agricultural use of chlorpyrifos in the Pampean region, Argentina. (bioportfolio.com)
  • We describe the concept of effective conjugation length, and its relationship to the S 0 → S 2 electronic transition, the decay rate of the S 1 energetic level and the frequency of the ν 1 Raman band. (royalsocietypublishing.org)
  • You can also optionally choose a column for Frequency/Wavelength , which will be used for labels in Loading Plot , choose Spectra Names for labels in Scor Plot , and Group Info can be used to divide samples in Score Plot . (originlab.com)
  • An early Raman spectrum of benzene published by Raman and Krishnan. (wikipedia.org)
  • This study is to evaluate the capability of Raman spectroscopy as a non-invasive method for monitoring concentrations of glucose by illuminating a small spot on the skin, collecting and an. (bioportfolio.com)
  • By the end of the second reporting period, the MOON 2 platform has been finalized, incorporating now, in addition to the fluorescence and OCT channels, the specifically designed Raman channel, as well as a tracking capability. (europa.eu)
  • Having the capability to perform photoluminescence and Raman spectroscopies simultaneously with the same instrument is advantageous, especially when studying two-dimensional (2D) crystals. (horiba.com)
  • Raman spectroscopy can be used to determine the temperature of a material when other more direct means are either impractical or not possible. (horiba.com)
  • New insights of Raman spectroscopy for oral clinical applications. (bioportfolio.com)
  • Analysis of key clinical features for achieving complete remission in stage III and IV non-small cell lung cancer patients. (nih.gov)
  • A Raman reference database is stepwise being compiled and serves as backbone for the chemometric analysis of more complex spectra. (europa.eu)
  • Raman spectroscopy is a process to unambiguously identify and differentiate various materials from one another. (eurekalert.org)
  • Then linear discriminant analysis (LDA) was utilized to differentiate the loading score of different diseases as the diagnosing algorithm. (spie.org)
  • The collected spectral data were used to build a discriminant analysis model that identified the effects of unknown antibiotic compounds on the phenotype of E. coli cultures. (asm.org)
  • Selected Raman Spectral Data: American Petroleum Institute Research Project 44. (barnesandnoble.com)