A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)
Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.
Technique whereby the weight of a sample can be followed over a period of time while its temperature is being changed (usually increased at a constant rate).
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Method of using a polycrystalline powder and Rietveld refinement (LEAST SQUARES ANALYSIS) of X-RAY DIFFRACTION or NEUTRON DIFFRACTION. It circumvents the difficulties of producing single large crystals.
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Rhodopsins found in the PURPLE MEMBRANE of halophilic archaea such as HALOBACTERIUM HALOBIUM. Bacteriorhodopsins function as an energy transducers, converting light energy into electrochemical energy via PROTON PUMPS.
Devices for accelerating charged particles in a spiral path by a constant-frequency alternating electric field. This electric field is synchronized with the movement of the particles in a constant magnetic field.
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
A noninvasive technique that uses the differential absorption properties of hemoglobin and myoglobin to evaluate tissue oxygenation and indirectly can measure regional hemodynamics and blood flow. Near-infrared light (NIR) can propagate through tissues and at particular wavelengths is differentially absorbed by oxygenated vs. deoxygenated forms of hemoglobin and myoglobin. Illumination of intact tissue with NIR allows qualitative assessment of changes in the tissue concentration of these molecules. The analysis is also used to determine body composition.
Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The physical characteristics and processes of biological systems.
Relating to the size of solids.
Nanometer-scale composite structures composed of organic molecules intimately incorporated with inorganic molecules. (Glossary of Biotechnology and Nanobiotechology Terms, 4th ed)
The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.
The study of the energy of electrons ejected from matter by the photoelectric effect, i.e., as a direct result of absorption of energy from electromagnetic radiation. As the energies of the electrons are characteristic of a specific element, the measurement of the energy of these electrons is a technique used to determine the chemical composition of surfaces.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Characteristics or attributes of the outer boundaries of objects, including molecules.
A colloidal, hydrated aluminum silicate that swells 12 times its dry size when added to water.
The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)
The isotopic compound of hydrogen of mass 2 (deuterium) with oxygen. (From Grant & Hackh's Chemical Dictionary, 5th ed) It is used to study mechanisms and rates of chemical or nuclear reactions, as well as biological processes.
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A species of halophilic archaea found in salt lakes. Some strains form a PURPLE MEMBRANE under anaerobic conditions.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
The spectrometric analysis of fluorescent X-RAYS, i.e. X-rays emitted after bombarding matter with high energy particles such as PROTONS; ELECTRONS; or higher energy X-rays. Identification of ELEMENTS by this technique is based on the specific type of X-rays that are emitted which are characteristic of the specific elements in the material being analyzed. The characteristic X-rays are distinguished and/or quantified by either wavelength dispersive or energy dispersive methods.
The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.
Removal of moisture from a substance (chemical, food, tissue, etc.).
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Condensation products of aromatic amines and aldehydes forming azomethines substituted on the N atom, containing the general formula R-N:CHR. (From Grant & Hackh's Chemical Dictionary, 5th ed)
A subclass of heme a containing cytochromes that have two imidazole nitrogens as axial ligands and an alpha-band absorption of 605 nm. They are found in a variety of microorganisms and in eucaryotes as a low-spin cytochrome component of MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.
A subclass of heme a containing cytochromes with an alpha-band absorption of 605 nm. They are found in a variety of microorganisms and in eukaryotes as a high-spin cytochrome component of MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Poly-2-methylpropenoic acids. Used in the manufacture of methacrylate resins and plastics in the form of pellets and granules, as absorbent for biological materials and as filters; also as biological membranes and as hydrogens. Synonyms: methylacrylate polymer; poly(methylacrylate); acrylic acid methyl ester polymer.
Deuterium. The stable isotope of hydrogen. It has one neutron and one proton in the nucleus.
Seven-carbon saturated hydrocarbon group of the methane series. Include isomers and derivatives.
A group of phosphate minerals that includes ten mineral species and has the general formula X5(YO4)3Z, where X is usually calcium or lead, Y is phosphorus or arsenic, and Z is chlorine, fluorine, or OH-. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Pollution prevention through the design of effective chemical products that have low or no toxicity and use of chemical processes that reduce or eliminate the use and generation of hazardous substances.
The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)
Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
A continuing periodic change in displacement with respect to a fixed reference. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A change of a substance from one form or state to another.
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
The characteristic three-dimensional shape of a molecule.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.
Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A synthetic phospholipid used in liposomes and lipid bilayers for the study of biological membranes.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
The rate dynamics in chemical or physical systems.
Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.
Measurement of the intensity and quality of fluorescence.
A conjugated protein which is the oxygen-transporting pigment of muscle. It is made up of one globin polypeptide chain and one heme group.
Carbon monoxide (CO). A poisonous colorless, odorless, tasteless gas. It combines with hemoglobin to form carboxyhemoglobin, which has no oxygen carrying capacity. The resultant oxygen deprivation causes headache, dizziness, decreased pulse and respiratory rates, unconsciousness, and death. (From Merck Index, 11th ed)
Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
Devices for accelerating protons or electrons in closed orbits where the accelerating voltage and magnetic field strength varies (the accelerating voltage is held constant for electrons) in order to keep the orbit radius constant.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Mathematical procedure that transforms a number of possibly correlated variables into a smaller number of uncorrelated variables called principal components.
Chemical bond cleavage reactions resulting from absorption of radiant energy.
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Dosage forms of a drug that act over a period of time by controlled-release processes or technology.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
Salts or ions of the theoretical carbonic acid, containing the radical CO2(3-). Carbonates are readily decomposed by acids. The carbonates of the alkali metals are water-soluble; all others are insoluble. (From Grant & Hackh's Chemical Dictionary, 5th ed)
The quality or state of being wettable or the degree to which something can be wet. This is also the ability of any solid surface to be wetted when in contact with a liquid whose surface tension is reduced so that the liquid spreads over the surface of the solid.
Organic compounds containing the -CO-NH2 radical. Amides are derived from acids by replacement of -OH by -NH2 or from ammonia by the replacement of H by an acyl group. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
A principle of estimation in which the estimates of a set of parameters in a statistical model are those quantities minimizing the sum of squared differences between the observed values of a dependent variable and the values predicted by the model.
A nitrogen-free class of lipids present in animal and particularly plant tissues and composed of one mole of glycerol and 1 or 2 moles of phosphatidic acid. Members of this group differ from one another in the nature of the fatty acids released on hydrolysis.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
One of the non-essential amino acids commonly occurring in the L-form. It is found in animals and plants, especially in sugar cane and sugar beets. It may be a neurotransmitter.
That portion of the electromagnetic spectrum usually sensed as heat. Infrared wavelengths are longer than those of visible light, extending into the microwave frequencies. They are used therapeutically as heat, and also to warm food in restaurants.
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
Chemical reaction in which monomeric components are combined to form POLYMERS (e.g., POLYMETHYLMETHACRYLATE).
A nonionic polyoxyethylene-polyoxypropylene block co-polymer with the general formula HO(C2H4O)a(-C3H6O)b(C2H4O)aH. It is available in different grades which vary from liquids to solids. It is used as an emulsifying agent, solubilizing agent, surfactant, and wetting agent for antibiotics. Poloxamer is also used in ointment and suppository bases and as a tablet binder or coater. (Martindale The Extra Pharmacopoeia, 31st ed)
Nanometer-sized, hollow, spherically-shaped objects that can be utilized to encapsulate small amounts of pharmaceuticals, enzymes, or other catalysts (Glossary of Biotechnology and Nanobiotechnology, 4th ed).
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The chemical and physical integrity of a pharmaceutical product.
A genus of rod-shaped, extremely halophilic HALOBACTERIACEAE which grows in alkaline conditions. They are strictly aerobic and some strains are motile. Natronobacterium is found in soda lakes, alkaline salterns, and soda soils.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.
Synthetic phospholipid used in liposomes and lipid bilayers to study biological membranes. It is also a major constituent of PULMONARY SURFACTANTS.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
The thermodynamic interaction between a substance and WATER.
A large multisubunit protein complex found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to catalyze the splitting of WATER into DIOXYGEN and of reducing equivalents of HYDROGEN.
A genus of HALOBACTERIACEAE whose growth requires a high concentration of salt. Binary fission is by constriction.
A chelating agent used as an antidote to heavy metal poisoning.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Hyaline cartilages in the nose. There are five major nasal cartilages including two lateral, two alar, and one septal.
A polyvinyl polymer of variable molecular weight; used as suspending and dispersing agent and vehicle for pharmaceuticals; also used as blood volume expander.
The mechanical property of material that determines its resistance to force. HARDNESS TESTS measure this property.
The fruiting 'heads' or 'caps' of FUNGI, which as a food item are familiarly known as MUSHROOMS, that contain the FUNGAL SPORES.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Extracellular substance of bone tissue consisting of COLLAGEN fibers, ground substance, and inorganic crystalline minerals and salts.
Computer-assisted processing of electric, ultrasonic, or electronic signals to interpret function and activity.
Elements of limited time intervals, contributing to particular results or situations.
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
Substances that cause the adherence of two surfaces. They include glues (properly collagen-derived adhesives), mucilages, sticky pastes, gums, resins, or latex.
A non-aqueous co-solvent that serves as tool to study protein folding. It is also used in various pharmaceutical, chemical and engineering applications.
Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.
A purplish-red, light-sensitive pigment found in RETINAL ROD CELLS of most vertebrates. It is a complex consisting of a molecule of ROD OPSIN and a molecule of 11-cis retinal (RETINALDEHYDE). Rhodopsin exhibits peak absorption wavelength at about 500 nm.
The reaction product of bisphenol A and glycidyl methacrylate that undergoes polymerization when exposed to ultraviolet light or mixed with a catalyst. It is used as a bond implant material and as the resin component of dental sealants and composite restorative materials.
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.
Substances made up of an aggregation of small particles, as that obtained by grinding or trituration of a solid drug. In pharmacy it is a form in which substances are administered. (From Dorland, 28th ed)
Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.
Tailored macromolecules harboring covalently-bound biologically active modules that target specific tissues and cells. The active modules or functional groups can include drugs, prodrugs, antibodies, and oligonucleotides, which can act synergistically and be multitargeting.
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
Protein complexes that take part in the process of PHOTOSYNTHESIS. They are located within the THYLAKOID MEMBRANES of plant CHLOROPLASTS and a variety of structures in more primitive organisms. There are two major complexes involved in the photosynthetic process called PHOTOSYSTEM I and PHOTOSYSTEM II.
The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed)
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
Signal and data processing method that uses decomposition of wavelets to approximate, estimate, or compress signals with finite time and frequency domains. It represents a signal or data in terms of a fast decaying wavelet series from the original prototype wavelet, called the mother wavelet. This mathematical algorithm has been adopted widely in biomedical disciplines for data and signal processing in noise removal and audio/image compression (e.g., EEG and MRI).
Particles consisting of aggregates of molecules held loosely together by secondary bonds. The surface of micelles are usually comprised of amphiphatic compounds that are oriented in a way that minimizes the energy of interaction between the micelle and its environment. Liquids that contain large numbers of suspended micelles are referred to as EMULSIONS.
The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
An acid dye used in testing for hydrochloric acid in gastric contents. It is also used histologically to test for AMYLOIDOSIS.
Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.
A very strong halogenated derivative of acetic acid. It is used in acid catalyzed reactions, especially those where an ester is cleaved in peptide synthesis.
Inorganic salts of phosphoric acid.
The branch of medicine concerned with the application of NANOTECHNOLOGY to the prevention and treatment of disease. It involves the monitoring, repair, construction, and control of human biological systems at the molecular level, using engineered nanodevices and NANOSTRUCTURES. (From Freitas Jr., Nanomedicine, vol 1, 1999).
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
Compounds similar to hydrocarbons in which a tetravalent silicon atom replaces the carbon atom. They are very reactive, ignite in air, and form useful derivatives.
Light driven chloride ion pumps that are ubiquitously found in halophilic archaea (HALOBACTERIALES).
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.
A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.
A type of massage in which finger pressure on specific body sites is used to promote healing, relieve fatigue, etc. Although the anatomical locations are the same as the ACUPUNCTURE POINTS used in ACUPUNCTURE THERAPY (hence acu-), no needle or other acupuncture technique is employed in acupressure. (From Random House Unabridged Dictionary, 2d ed). Shiatsu is a modern outgrowth that focuses more on prevention than healing.
Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.
A statistical analytic technique used with discrete dependent variables, concerned with separating sets of observed values and allocating new values. It is sometimes used instead of regression analysis.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to an ethanolamine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and ethanolamine and 2 moles of fatty acids.
A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.
Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.
Computer-based representation of physical systems and phenomena such as chemical processes.
Agents that modify interfacial tension of water; usually substances that have one lipophilic and one hydrophilic group in the molecule; includes soaps, detergents, emulsifiers, dispersing and wetting agents, and several groups of antiseptics.
Liquids transforming into solids by the removal of heat.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A widely cultivated plant, native to Asia, having succulent, edible leaves eaten as a vegetable. (From American Heritage Dictionary, 1982)
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
A species of gram-negative, aerobic, rod-shaped bacteria found in hot springs of neutral to alkaline pH, as well as in hot-water heaters.
Any visual display of structural or functional patterns of organs or tissues for diagnostic evaluation. It includes measuring physiologic and metabolic responses to physical and chemical stimuli, as well as ultramicroscopy.
A highly caustic substance that is used to neutralize acids and make sodium salts. (From Merck Index, 11th ed)
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
Submicron-sized fibers with diameters typically between 50 and 500 nanometers. The very small dimension of these fibers can generate a high surface area to volume ratio, which makes them potential candidates for various biomedical and other applications.
A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

Polarization-modulated FTIR spectroscopy of lipid/gramicidin monolayers at the air/water interface. (1/3535)

Monolayers of gramicidin A, pure and in mixtures with dimyristoylphosphatidylcholine (DMPC), were studied in situ at the air/H2O and air/D2O interfaces by polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). Simulations of the entire set of amide I absorption modes were also performed, using complete parameter sets for different conformations based on published normal mode calculations. The structure of gramicidin A in the DMPC monolayer could clearly be assigned to a beta6.3 helix. Quantitative analysis of the amide I bands revealed that film pressures of up to 25-30 mN/m the helix tilt angle from the vertical in the pure gramicidin A layer exceeded 60 degrees. A marked dependence of the peptide orientation on the applied surface pressure was observed for the mixed lipid-peptide monolayers. At low pressure the helix lay flat on the surface, whereas at high pressures the helix was oriented almost parallel to the surface normal.  (+info)

Distortion of the L-->M transition in the photocycle of the bacteriorhodopsin mutant D96N: a time-resolved step-scan FTIR investigation. (2/3535)

The D96N mutant of bacteriorhodopsin has often been taken as a model system to study the M intermediate of the wild type photocycle due to the long life time of the corresponding intermediate of the mutant. Using time-resolved step-scan FTIR spectroscopy in combination with a sample changing wheel we investigated the photocycle of the mutant with microsecond time resolution. Already after several microseconds an intermediate similar to the MN state is observed, which contrasts with the M state of the wild type protein. At reduced hydration M and N intermediates similar to those of wild type BR can be detected. These results have a bearing on the interpretation of the photocycle of this mutant. A mechanism is suggested for the fast rise of MN which provides some insight into the molecular events involved in triggering the opening of the cytosolic channel also of the wild type protein.  (+info)

Both familial Parkinson's disease mutations accelerate alpha-synuclein aggregation. (3/3535)

Parkinson's disease (PD) is a neurodegenerative disorder that is pathologically characterized by the presence of intracytoplasmic Lewy bodies, the major component of which are filaments consisting of alpha-synuclein. Two recently identified point mutations in alpha-synuclein are the only known genetic causes of PD, but their pathogenic mechanism is not understood. Here we show that both wild type and mutant alpha-synuclein form insoluble fibrillar aggregates with antiparallel beta-sheet structure upon incubation at physiological temperature in vitro. Importantly, aggregate formation is accelerated by both PD-linked mutations. Under the experimental conditions, the lag time for the formation of precipitable aggregates is about 280 h for the wild type protein, 180 h for the A30P mutant, and only 100 h for the A53T mutant protein. These data suggest that the formation of alpha-synuclein aggregates could be a critical step in PD pathogenesis, which is accelerated by the PD-linked mutations.  (+info)

Chloride ion binding to bacteriorhodopsin at low pH: an infrared spectroscopic study. (4/3535)

Bacteriorhodopsin (bR) and halorhodopsin (hR) are light-induced ion pumps in the cell membrane of Halobacterium salinarium. Under normal conditions bR is an outward proton transporter, whereas hR is an inward Cl- transporter. There is strong evidence that at very low pH and in the presence of Cl-, bR transports Cl- ions into the cell, similarly to hR. The chloride pumping activity of bR is connected to the so-called acid purple state. To account for the observed effects in bR a tentative complex counterion was suggested for the protonated Schiff base of the retinal chromophore. It would consist of three charged residues: Asp-85, Asp-212, and Arg-82. This quadruplet (including the Schiff base) would also serve as a Cl- binding site at low pH. We used Fourier transform infrared difference spectroscopy to study the structural changes during the transitions between the normal, acid blue, and acid purple states. Asp-85 and Asp-212 were shown to participate in the transitions. During the normal-to-acid blue transition, Asp-85 protonates. When the pH is further lowered in the presence of Cl-, Cl- binds and Asp-212 also protonates. The binding of Cl- and the protonation of Asp-212 occur simultaneously, but take place only when Asp-85 is already protonated. It is suggested that HCl is taken up in undissociated form in exchange for a neutral water molecule.  (+info)

Structural analysis of DNA-chlorophyll complexes by Fourier transform infrared difference spectroscopy. (5/3535)

Porphyrins and metalloporphyrins are strong DNA binders. Some of these compounds have been used for radiation sensitization therapy of cancer and are targeted to interact with cellular DNA. This study was designed to examine the interaction of calf thymus DNA with chlorophyll a (CHL) in aqueous solution at physiological pH with CHL/DNA(phosphate) ratios (r) of 1/160, 1/80, 1/40, 1/20, 1/10, and 1/5. Fourier transform infrared (FTIR) difference spectroscopy was used to characterize the nature of DNA-pigment interactions and to establish correlations between spectral changes and the CHL binding mode, binding constant, sequence selectivity, DNA secondary structure, and structural variations of DNA-CHL complexes in aqueous solution. Spectroscopic results showed that CHL is an external DNA binder with no affinity for DNA intercalation. At low pigment concentration (r = 1/160, 1/80, and 1/40), there are two major binding sites for CHL on DNA duplex: 1) Mg-PO2 and 2) Mg-N7 (guanine) with an overall binding constant of K = 1.13 x 10(4) M-1. The pigment distributions are 60% with the backbone PO2 group and 20% with the G-C base pairs. The chlorophyll interaction is associated with a major reduction of B-DNA structure in favor of A-DNA. At high chlorophyll content (r = 1/10), helix opening occurs, with major spectral alterations of the G-C and A-T bases. At high chlorophyll concentration (1/5), pigment aggregation is observed, which does not favor CHL-DNA complexation.  (+info)

Spectral morphometric characterization of breast carcinoma cells. (6/3535)

The spectral morphometric characteristics of standard haematoxylin and eosin breast carcinoma specimens were evaluated by light microscopy combined with a spectral imaging system. Light intensity at each wavelength in the range of 450-800 nm was recorded for 10(4) pixels from each field and represented as transmitted light spectra. A library of six characteristic spectra served to scan the cells and reconstruct new images depicting the nuclear area occupied by each spectrum. Fifteen cases of infiltrating ductal carcinoma and six cases of lobular carcinoma were examined; nine of the infiltrating ductal carcinoma and three of the lobular carcinoma showed an in situ component. The spectral morphometric analysis revealed a correlation between specific patterns of spectra and different groups of breast carcinoma cells. The most consistent result was that lobular carcinoma cells of in situ and infiltrating components from all patients showed a similar spectral pattern, whereas ductal carcinoma cells displayed spectral variety. Comparison of the in situ and the infiltrating ductal solid, cribriform and comedo carcinoma cells from the same patient revealed a strong similarity of the spectral elements and their relative distribution in the nucleus. The spectrum designated as number 5 in the library incorporated more than 40% of the nuclear area in 74.08% of the infiltrating lobular cells and in 13.64% of the infiltrating ductal carcinoma cells (P < 0.001). Spectrum number 2 appeared in all infiltrating ductal cells examined and in none of the lobular cells. These results indicate that spectrum number 5 is related to infiltrating lobular carcinoma, whereas spectrum number 2 is characteristic for infiltrating ductal carcinoma cells. Spectral similarity mapping of central necrotic regions of comedo type in situ carcinoma revealed nuclear fragmentation into defined segments composed of highly condensed chromatin. We conclude that the spectral morphometric features found for lobular and ductal cell populations may serve future automated histological diagnostics.  (+info)

Membrane interactions of the synthetic N-terminal peptide of HIV-1 gp41 and its structural analogs. (7/3535)

Structural and functional studies assessed the membrane actions of the N terminus of HIV-1 glycoprotein 41000 (gp41). Earlier site-directed mutagenesis has shown that key amino acid changes in this gp41 domain inhibit viral infection and syncytia formation. Here, a synthetic peptide corresponding to the N terminus of gp41 (FP; 23 residues, 519-541), and also FP analogs (FP520V/E with Val-->Glu at residue 520; FP527L/R with Leu-->Arg at 527; FP529F/Y with Phe-->Tyr at 529; and FPCLP1 with FP truncated at 525) incorporating these modifications were prepared. When added to human erythrocytes at physiologic pH, the lytic and aggregating activities of the FP analogs were much reduced over those with the wild-type FP. With resealed human erythrocyte ghosts, the lipid-mixing activities of the FP analogs were also substantially depressed over that with the wild-type FP. Combined with results from earlier studies, theoretical calculations using hydrophobic moment plot analysis and physical experiments using circular dichroism and Fourier transform infrared spectroscopy indicate that the diminished lysis and fusion noted for FP analogs may be due to altered peptide-membrane lipid interactions. These data confirm that the N-terminal gp41 domain plays critical roles in the cytolysis and fusion underlying HIV-cell infection.  (+info)

Substitution of the methionine residues of calmodulin with the unnatural amino acid analogs ethionine and norleucine: biochemical and spectroscopic studies. (8/3535)

Calmodulin (CaM) is a 148-residue regulatory calcium-binding protein that activates a wide range of target proteins and enzymes. Calcium-saturated CaM has a bilobal structure, and each domain has an exposed hydrophobic surface region where target proteins are bound. These two "active sites" of calmodulin are remarkably rich in Met residues. Here we have biosynthetically substituted (up to 90% incorporation) the unnatural amino acids ethionine (Eth) and norleucine (Nle) for the nine Met residues of CaM. The substituted proteins bind in a calcium-dependent manner to hydrophobic matrices and a synthetic peptide, encompassing the CaM-binding domain of myosin light-chain kinase (MLCK). Infrared and circular dichroism spectroscopy show that there are essentially no changes in the secondary structure of these proteins compared to wild-type CaM (WT-CaM). One- and two-dimensional NMR studies of the Eth-CaM and Nle-CaM proteins reveal that, while the core of the proteins is relatively unaffected by the substitutions, the two hydrophobic interaction surfaces adjust to accommodate the Eth and Nle residues. Enzyme activation studies with MLCK show that Eth-CaM and Nle-CaM activate the enzyme to 90% of its maximal activity, with little changes in dissociation constant. For calcineurin only 50% activation was obtained, and the K(D) for Nle-CaM also increased 3.5-fold compared with WT-CaM. These data show that the "active site" Met residues of CaM play a distinct role in the activation of different target enzymes, in agreement with site-directed mutagenesis studies of the Met residues of CaM.  (+info)

On-line attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy and focused-beam reflectance measurement (FBRM) were used to monitor and control anti-solvent crystallization of paracetamol from an acetone-water mixture, which was conducted isothermally in a 1-L crystallizer with a flat bottom. After analyzing the particle size distribution (PSD) and transient relative supersaturation data from constant anti-solvent addition rate experiments, a simple calculation method for the set point of anti-solvent addition rate, to maintain constant supersaturation via ATR-FTIR, was developed and implemented for feedback control of unseeded and seeded crystallization. The results of the controlled feeding rate experiments show that the particle size and PSD, as well as the total batch time, can be favorably manipulated simultaneously. © 2006 American Chemical Society ...
The amino-terminal extremity of the human immunodeficiency virus type 1 transmembrane protein (gp41) is thought to play a pivotal role in the fusion of virus membranes with the plasma membrane of the target cell and in syncytium formation. Peptides with sequences taken from the human immunodeficiency virus type 1 gp41 fusogenic (synthetic peptides SPwt and SP-2) and nonfusogenic (SP-3 and SP-4) glycoproteins adopt mainly a beta-sheet conformation in the absence of lipid, as determined by attenuated total reflection Fourier transform infrared spectroscopy, and after interaction with large unilamellar liposomes, the beta-sheet is partly converted into an alpha-helical conformation. Peptides SPwt and SP-2 but not SP-3 or SP-4 were able to promote lipid mixing as assessed by fluorescence energy transfer assay and dye leakage in a vesicle leakage assay. By using polarized attenuated total reflection Fourier transform infrared spectroscopy, SPwt and SP-2 were found to adopt an oblique orientation in ...
Superabsorbent composites based on chitosan-g-poly(acrylamide) and montorillonite (CTS-g-PAAm/MMT) were synthesized through in situ radical polymerization by grafting of crosslinked acrylamide onto chitosan backbone in presence of MMT at different contents. The formation of the grafted network was confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA), and differential scanning calorimetery (DSC). The obtained porous structure was observed by scanning electron microscope (SEM). The presence of clay and its interaction with chitosan-g-poly(acrylamide) (CTS-g-PAAm) matrix was evidenced by ATR-FTIR analysis. The morphology was investigated by both X-ray diffraction (XRD) and SEM analyses. It was suggested the formation of mostly exfoliated structures with more porous structures. Besides, the thermal stability of these composites, observed by TGA analysis, was slightly affected by the clay loading as compared to the matrix. ...
In this study, hierarchical cluster analysis (HCA) and principal component analysis (PCA) were used to classify blends produced from diesel S500 and different kinds of biodiesel produced by the TDSP methodology. The different kinds of biodiesel studied in this work were produced from three raw materials: soybean oil, waste cooking oil and hydrogenated vegetable oil. Methylic and ethylic routes were employed for the production of biodiesel. HCA and PCA were performed on the data from attenuated total reflectance Fourier transform infrared spectroscopy, showing the separation of the blends into groups according to biodiesel content present in the blends and to the kind of biodiesel used to form the mixtures ...
We have used three dimensional (3D) extrusion printing to manufacture a multi-active solid dosage form or so called polypill. This contains five compartmentalised drugs with two independently controlled and well-defined release profiles. This polypill demonstrates that complex medication regimes can be combined in a single personalised tablet. This could potentially improve adherence for those patients currently taking many separate tablets and also allow ready tailoring of a particular drug combination/drug release for the needs of an individual. The polypill here represents a cardiovascular treatment regime with the incorporation of an immediate release compartment with aspirin and hydrochlorothiazide and three sustained release compartments containing pravastatin, atenolol, and ramipril. X-ray powder diffraction (XRPD) and Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) were used to assess drug-excipient interaction. The printed polypills were evaluated for ...
TY - JOUR. T1 - Influence of hydrolytic degradation on the surface properties of poly-5d/95l-lactide resorbable bone plates. AU - Hu, Hsin Tai. AU - Shin, Tsai Chin. AU - Lee, Sheng Yang. AU - Chen, Chien Chung. AU - Yang, Jen Chang. PY - 2011/8. Y1 - 2011/8. N2 - This study explores in vitro aging effects on the surface properties of resorbable PLA95 (poly-5d/95l-lactide) bone plates. The in vitro degradation of injection molded PLA95 bone plates was undertaken by soaking them in a PBS solution. Specimens were harvested at 0, 4, 6, 8, 12, 20, and 26 weeks. After each in vitro aging period, the surface morphology, viscosity, chemical structure, wettability, and thermal properties of the PLA95 bone plates were examined by scanning electron microscopy (SEM), capillary viscometers, attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR), contact angle, and modulated differential scanning calorimetry (MDSC), respectively. The surface morphology of aged PLA95 bone plates ...
in Journal of Protein Chemistry (1994), 13(1), 77-88. Peptides corresponding to lipid binding domains of Apo B-100 were synthesized, purified, and incubated with dimyristoylphosphatidylcholine (DMPC) liposomes. The secondary structure of the apo B-100 ... [more ▼]. Peptides corresponding to lipid binding domains of Apo B-100 were synthesized, purified, and incubated with dimyristoylphosphatidylcholine (DMPC) liposomes. The secondary structure of the apo B-100 peptide-lipid complexes was evaluated by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Those peptides belonging to the hydrophobic core domain of apo B-100 when associated with phospholipids were rich in beta sheet structure; a predominant alpha helical conformation was shown to be associated with one peptide located in a surface region of apo B-100. IR dichroic spectra revealed, in the case of the core peptides, that the beta sheet component is the only oriented structure with respect to the ...
Previous studies revealed that pesticides interact with α-synuclein and accelerate the rate of fibrillation. These results are consistent with the prevailing hypothesis that the direct interaction of α-synuclein with pesticides is one of many suspected factors leading to α-synuclein fibrillation and ultimately to Parkinsons disease. In this study, the biophysical properties and fibrillation kinetics of α-synuclein in the presence of rotenone were investigated and, more specifically, the effects of rotenone on the early-stage misfolded forms of α-synuclein were considered. The thioflavine T (ThT) fluorescence assay studies provide evidence that early-phase misfolded α-synuclein forms are affected by rotenone and that the fibrillation process is accelerated. Further characterization by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) shows that rotenone increases the amount of ordered secondary structure in this intrinsically disordered protein. Morphological
TY - JOUR. T1 - Salivary molecular spectroscopy. T2 - a sustainable, rapid and non-invasive monitoring tool for diabetes mellitus during insulin treatment. AU - Caixeta, Douglas C.. AU - Aguiar, Emília M. G.. AU - Cardoso-Sousa, Léia. AU - Coelho, Líris M. D.. AU - Oliveira, Stephanie W.. AU - Espindola, Foued S.. AU - Raniero, Leandro. AU - Crosara, Karla T. B.. AU - Baker, Matthew J.. AU - Siqueira, Walter L.. AU - Sabino-Silva, Robinson. PY - 2020/3/17. Y1 - 2020/3/17. N2 - Monitoring of blood glucose is an invasive, painful and costly practice in diabetes. Consequently, the search for a more cost-effective (reagent-free), non-invasive and specific diabetes monitoring method is of great interest. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy has been used in diagnosis of several diseases, however, applications in the monitoring of diabetic treatment are just beginning to emerge. Here, we used ATR-FTIR spectroscopy to evaluate saliva of non-diabetic (ND), ...
Cellulose nanocrystals (CNC) were successfully obtained and modified with 1,6-hexamethylene diisocyanate (HE)!) by means of in situ polymerization varying the CNC/HDI molar ratio to evaluate the number of anchored chains to the CNC. The modification was examined by elemental analysis, nuclear magnetic resonance ((13)C NMR) and attenuated total reflection Fourier transform infrared spectroscopy (IR-ATR). Nanocomposites containing 1.5 wt% CNC, modified and unmodified, were prepared by solvent casting. Thermal and mechanical properties of the resulting films were evaluated from the viewpoint of polyurethane microphase separated structure, soft and hard domains. CNC were effectively dispersed in the polyurethane matrix and depending on surface chemistry, the nanoreinforcement interacts selectively with matrix nanodomains. This interpretation is supported by differences in thermal and mechanical properties of the nanocomposites and also confirmed by AFM images. Isocyanate rich cellulose nanocrystals ...
On the molecular level, injectable fillers are designed to last different lengths of time, and can be categorized as short (0-3 months), medium (3-12 months), or long-term (12 months to indefinitely) [3]. Collagen, hyaluronic acid, and silicone are examples of commonly used short, medium, and long-term fillers, respectively. Generally speaking, shorter-term products have greater biocompatibility [4]. Depending on the type of filler used, complication rates range from 3 % to 52 % [5]. In the short term, adverse events can include bleeding, infections, edema, and migration. In the long term, there is a risk of lump formation, granulomas, abscesses, more diffuse edema, and skin discoloration [2]. In these cases, having a reliable way to identify unknown substances prior to surgical treatment would be of great value both medically and legally [6]. Attenuated total reflectance/Fourier transform infrared spectroscopy can accurately identify unknown injectable fillers [6]. However, this method requires ...
Long-term occupational exposure to low level of fluoride can induce oxidative stress and apoptosis in many cells, including lymphocyte. However, the underlying mechanism remains unclear. Hence, this study was designed to explore the potential oxidative stress and apoptosis of long-term occupational exposure to low level of fluoride in aluminum smelter workers. A total of 120 aluminum smelter workers were recruited in control, low-, middle-, and high-fluoride exposure groups with 30 workers for each group. The peripheral blood samples were collected, centrifuged, and isolated to obtain serum and lymphocyte suspensions. The air and serum fluoride concentrations were detected by fluoride ion-selective electrode method. The lymphocytic apoptosis rate, DNA damage, oxidative stress, and mRNA levels of p53, Bcl-2, and Bax were assessed by Annexin V/PI staining, comet assay, attenuated total reflectance Fourier transform infrared spectroscopy and real-time polymerase chain reaction, respectively. ...
The relationship between cellular adhesion and surface roughness for polyurethane modified by microwave plasma radiation Saeed Heidari Keshel1, S Neda Kh Azhdadi2, Azadeh Asefnezhad2, Mohammad Sadraeian3, Mohamad Montazeri4, Esmaeil Biazar51Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences; 2Department of Biomaterial Engineering, Faculty of Biomedical Engineering, Science and Research Branch - Islamic Azad University; 3Young Researchers Club, Islamic Azad University, North Tehran Branch, Tehran; 4Faculty of Medical Sciences, Babol University of Medical Sciences, Babol; 5Department of Chemistry, Islamic Azad University, Tonekabon, IranAbstract: Surface modification of medical polymers is carried out to improve biocompatibility. In this study, conventional polyurethane was exposed to microwave plasma treatment with oxygen and argon gases for 30 seconds and 60 seconds. Attenuated total reflection Fourier transform infrared spectra investigations of
LmrP is an electrogenic H(+)/drug antiporter that extrudes a broad spectrum of antibiotics. Five carboxylic residues are implicated in drug binding (Asp142 and Glu327) and proton motive force-mediated restructuring (Asp68, Asp128 and Asp235). ATR-FTIR (Attenuated Total Reflection - Fourier Transform Infrared) and tryptophan quenching experiments revealed that phosphatidylethanolamine (PE) is required to generate the structural intermediates induced by ionization of carboxylic residues. Surprisingly, no ionization-induced conformational changes were detectable in the absence of PE, suggesting either that carboxylic acid residues do not ionize or that ionization does not lead to any conformational change. The mean pKa of carboxylic residues evaluated by ATR-FTIR spectroscopy was 6.5 for LmrP reconstituted in PE liposomes, whereas the pKa calculated in the absence of PE was 4.6. Considering that 16 of the 19 carboxylic residues are located in the extramembrane loops, the pKa values obtained in the ...
Abstract This paper proposes a direct and efficient method to discriminate between counterfeit and authentic Cialis and Viagra samples by combining attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy with multivariate techniques. The chemical profile of 53 commercial samples (Viagra®, Cialis®) and 104 counterfeit samples (Viagra and Cialis) from distinct seizures were obtained from ATR-FTIR…. [...] ...
The competitive adsorption at the water-goethite interface between phosphate and a carboxylic acid, either oxalate, citrate, 1,2,3,4-butanetetracarboxylic acid (BTCA), mellitate or Suwannee River Standard Fulvic Acid 1S101F (FA), was investigated over a wide pH range (3-9) by means of batch experiments and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. The quantitative results from the competitive adsorption measurements show that the efficiency of the organic acids in competing with phosphate was in the order oxalate , citrate , BTCA FA , mellitate. Oxalate showed no detectable effect, whereas the effect in the mellitate system was strong, and the aggregative results indicate that an increasing number of carboxylic groups favours competitive ability towards phosphate. The infrared spectroscopic results show conclusively that competition for goethite surface sites between carboxylic acids and phosphate is not a ligand-exchange reaction between inner-sphere ...
TY - JOUR. T1 - The rapid diagnosis of gliomas via serum spectroscopy. AU - Baker, Matthew. PY - 2014/3. Y1 - 2014/3. N2 - Gliomas are the most frequent primary brain tumours in adults, with these intracranial neoplasms accounting for 70% of adult malignant brain tumours (1). Confirmatory diagnosis occurs by staining tumour sections and identifying microscopic features characteristic of a disease state. The current methodology is subjective, and may require the patient to undergo unnecessary surgery. Spectroscopy is the study of the absorption and emission of light. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy is rapid, cost-effective and analytical techniques and require little or no sample preparation or use of chemicals.. AB - Gliomas are the most frequent primary brain tumours in adults, with these intracranial neoplasms accounting for 70% of adult malignant brain tumours (1). Confirmatory diagnosis occurs by staining tumour sections and identifying ...
Iron, an essential nutrient, is primarily present in soils in the form of iron-bearing minerals characterized with low solubilities. Under iron deficient conditions, some plants and microorganisms exude a mixture of iron-complexing agents, including carboxylates and siderophores, that can cause minerals to dissolve and increase iron solubility. Siderophores are chelating agents with functional groups such as hydroxamate, catecholate, or α-hydroxycarboxylate, that have high selectivity and specificity for Fe(III). This thesis is focused on adsorption/dissolution processes at the surface of a common soil mineral, goethite(α-FeOOH), in the presence of oxalate and a trihydroxamate siderophore, desferrioxamine-B (DFOB) at pH 4 and/or 6 in the absence of visible light. In order to characterize these processes at a molecular level and to understand the reaction mechanisms, a combination of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, extended X-ray absorption fine ...
Alexandra Aloia, Peter Rampson, and Rosemarie C. Chinni, Elemental Analysis of Oil Paints Using Laser Induced Breakdown Spectroscopy (LIBS), Middle Atlantic Regional Meeting of the American Chemical Society (MARM), Forensic Chemistry Session, Hershey, PA, June 2017.*. Rosemarie C. Chinni, Incorporation of Laser Induced Breakdown Spectroscopy (LIBS) Into Analytical Chemistry/Instrumental Analysis and an Innovative Lab Experience For Students, Middle Atlantic Regional Meeting of the American Chemical Society (MARM), Innovations in Analytical Chemistry Education Session, Hershey, PA, June 2017.. Ian Evans and Rosemarie C. Chinni, Determination of Impurities in Over-the-Counter Pain Relievers with Common Illicit Drug Cutting Compounds Using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) Spectroscopy, HECBC, Penn State Berks University, Reading, PA, April 2017.*. Daniel Martin and Rosemarie C. Chinni, Using High Pressure Liquid Chromatography (HPLC) For the Analysis of ...
Primary Syrian hamster embryo (SHE) cells might be used to assess morphological transformation following treatment with chemical carcinogens. We employed attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy to interrogate SHE colonies, as complex biomolecules absorb in the mid-infrared (IR; λ = 2-20 μm) giving vibrational spectra associated with structure and function. Early-passage SHE cells were cultured (pH 6.7) in the presence or absence of benzo[a]pyrene (B[a]P; 5.0 μg/ml). Unstained colonies were applied to an ATR crystal, and vibrational spectra were obtained in the ATR mode using a Bruker Vector 22 FTIR spectrometer with Helios ATR attachment. These were individually baseline-corrected and normalised. Spectra were then analysed using principal component analysis (PCA) plus linear discriminant analysis (LDA). PCA was used to reduce the dataset dimensions before LDA was employed to reveal clustering. This determined whether wavenumber-absorbance relationships ...
The thermodynamically stable Form II of the antidiabetic drug tolbutamide exhibits a thin fiber needle shape which renders it intractable for isolation and downstream processing. This work implements two in situ process analytical technology (PAT) methods, namely, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) with orthogonal partial least-squares- principal component analysis (OPLS-PCA) for monitoring solute concentration, and Raman spectroscopy with dynamic PCA based multivariate statistical process monitoring (MSPM) for detection of solid form purity, to derive the robust design space for cooling crystallization of the desired Form IL. © 2011 American Chemical Society ...
A simple concept is proposed to metallise polyamide 66 (PA66) spherulite structures with in situ synthesised gold nanoparticles (Au NPs) using a wet chemical method. This cost-effective approach, applied to produce a PA66/Au NP hybrid material, offers the advantages of controlling the nanoparticle size, the size distribution and the organic-inorganic interactions. These are the key factors that have to be controlled to construct consistent Au nanostructures which are essential for producing the catalytic activities of interest. The hybrid materials obtained are characterised by means of scanning electron microscopy, transmission electron microscopy, attenuated total reflection-Fourier transform infrared spectrometry and X-ray diffraction spectrometry. The results show that PA66 microspheres obtained via the crystallisation process are coated with Au NPs of 13 nm in size. It was found that controlling the metal coordination is the key parameter to template the Au NPs on the spherulite surfaces. The
High performance liquid chromatography (HPLC) coupled with a Fraction Collector was employed to isolate Maillard reaction products (MRPs) formed in model systems comprising of asparagine and monosaccharides in the 60-180°C range. The primary MRP which is detected at 60°C is important for Acrylamide content and color/aroma development in foods and also in the field of food biotechnology for controlling the extent of the Maillard reaction with temperature. The discrete fractions of the reaction products were reacted with Hemoglobin (Hb) and Myoglobin (Mb) at physiological conditions and the reaction adducts were monitored by UV-vis and Attenuated Total Reflection-Fourier transform infrared (FTIR) spectrophotometry ...
TY - JOUR. T1 - Application of infrared attenuated total reflection spectroscopy to in situ analysis of atheromatous plaques in aorta. AU - Nakamura, Atsushi. AU - Koga, Takehiro. AU - Fujimaki, Makoto. AU - Ohki, Yoshimichi. AU - Sota, Takayuki. AU - Lipinska-Kalita, Kristina. AU - Nagae, Tsuneyuki. AU - Ishimaru, Shin. AU - Aizawa, Katsuo. PY - 2000. Y1 - 2000. N2 - Ex vivo infrared attenuated total reflection (ATR) spectroscopic studies have been carried out to observe the accumulation of cholesterol ester in atheromatous plaques. It has been found that ATR spectra can be successfully obtained from the intima side of aorta and give the same information as the transmission spectra. This means, the ATR spectra include information about the chemical composition of atheromatous plaques together with the degree of eventual lesions. We have demonstrated that ATR microspectroscopic measurements can give images of atheromatous plaques distribution and enable in situ observation of the relative amount ...
The Danish sculptor Anne Marie Carl-Nielsen (1863-1945) often prepared three-dimensional models in wax before producing her works of art. The Carl Nielsen Museum in Odense keeps a unique collection of around 200 of the artists fragile wax models. In 2008 the entire collection was examined, documented, X-ray radiographed, photographed, and conserved. Analyses of five figurines by attenuated total reflectance-Fourier transform infrared spectroscopy, optical microscopy, and scanning electron microscopy-energy dispersive X-ray spectroscopy showed two different kinds of wax mixtures, one containing beeswax and potato or maize starch as filler, and the other consisting of gypsum, zinc stearate, and a greasy substance. During the examination a specific deterioration phenomenon was noticed in the areas where the wax models had been strengthened with internal metal armatures. A chemical reaction between the wax mixture and the copper containing armature has caused an intense greenish colouring of the ...
Time-resolved Fourier transform infrared (TRFTIR) emission spectroscopy has been used to study the 193 nm photolysis of vinyl bromide (C(2)H(3)Br) and vinyl chloride (C(2)H(3)Cl). Time-resolved IR emission was analysed to obtain nascent vibrational state populations of two primary photolysis
Despite numerous advances in omics research, early detection of ovarian cancer still remains a challenge. The aim of this study was to determine whether attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman spectroscopy could characterise alterations in the biomolecular signatures of human blood plasma/serum obtained from ovarian cancer patients compared to non-cancer controls. Blood samples isolated from ovarian cancer patients (n = 30) and healthy controls (n = 30) were analysed using ATR-FTIR spectroscopy. For comparison, a smaller cohort of samples (n = 8) were analysed using an InVia Renishaw Raman spectrometer. Resultant spectra were pre-processed prior to being inputted into principal component analysis (PCA) and linear discriminant analysis (LDA). Statistically significant differences (P , 0.001) were observed between spectra of ovarian cancer versus control subjects for both biospectroscopy methods. Using a support vector machine classifier for Raman spectra of ...
Successful long term bone replacement and repair remain a challenge today. Nanotechnology has makes it possible to alter materials characteristics and therefore possibly improve on the material itself. In this study, biphasic (hydroxyapatite/β-tricalcium phosphate (HA/β-TCP)) nanobioceramic scaffolds were prepared by the electrospinning technique in order to mimic the extracellular matrix (ECM). Scaffolds were characterised by scanning electron microscopy (SEM) and Attentuated Total Reflectance Fourier Transform Infrared (ATRFTIR). Osteoblasts as well as monocytes that were differentiated into osteoclast-like cells, were cultured separately on the biphasic bioceramic scaffolds for up to 6 days and the proliferation, adhesion and cellular response were determined using lactate dehydrogenase (LDH) cytotoxicity assay, nucleus and cytoskeleton dynamics, analysis of the cell cycle progression, measurement of the mitochondrial membrane potential and the detection of phosphatidylserine expression. ...
Toxic responses to contaminants following exposure concentrations typically used in laboratory tests may not reflect how biological systems respond to lower environmental levels from which hormetic effect mechanisms have been suggested. We investigated the pattern of dose-response in mammalian cells to various environmental contaminants using a range of concentrations that span those that are environmentally relevant (10(-12) M to 10(-3) M). MCF-7 cell cultures were treated for 24 h with benzo[a]pyrene (B[a]P), lindane (gamma-hexachlorocyclohexane), or polybrominated diphenyl ethers (PBDEs) congeners (47, 153, 183, and 209), then fixed in ethanol and interrogated using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. Mode of action was further studied by examining if test agents stimulated cell growth or altered CYP1A1 expression. Bimodal dose response curves were observed when MCF-7 cells were treated with PBDEs or lindane. The first peak distribution was ...
The Fourier Transform Infrared Spectroscopy facility at the CEA Paris-Saclay is located at the Laboratory of Fundamental Mechanisms in Bioenergetics (UMR 9198). It provides the users with advanced FTIR spectrometry and responds to most of the needs of FTIR analyses.. The FTIR platform includes 4 spectrometers with many accessories: Transmission cell, ATR accessories, thermostatable liquid cells, cryostat for low temperature studies … It enables the analysis of various samples under different physical forms (see below). The laboratory has a specialization in FTIR difference spectroscopy, time-resolved FTIR, low temperature FTIR, and an expertise in the investigation of biochemical reactions and photo-induced reactions.. Equipment:. - Bruker Vertex 80V (under vacuum - to reduce noise and water vapor contamination; cryostat option; detectors: Bolometer, DTGS, MCT; spectral range between 100 and 6000 cm-1). It is interfaced with a pulsed laser Continuum Surelite+OPO (tunable between 440 and 670 ...
Graphene-based materials have been studied in a wide range of applications including catalysis due to the outstanding electronic, thermal, and mechanical properties. The unprecedented features of graphene-based catalysts, which are believed to be responsible for their superior performance, have been characterized by many techniques. In this article, we comprehensively summarized the characterization methods covering bulk and surface structure analysis, chemisorption ability determination, and reaction mechanism investigation. We reviewed the advantages/disadvantages of different techniques including Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Diffuse Reflectance Fourier Transform Infrared Spectroscopy (DRIFTS), X-Ray diffraction (XRD), X-ray absorption near edge structure (XANES) and X-ray absorption fine structure (XAFS), atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high
Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis, and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (?0.2 \ensuremathμm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this ...
Chemical contaminants, such as benzoapyrene (BaP), may modulate transcriptional responses in cells via the activation of aryl hydrocarbon receptor (AhR) or through responses to DNA damage following adduct formation. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy can be employed in a non-destructive fashion to interrogate the biochemical signature of cells via generation of infrared (IR) spectra. By applying to generated spectral datasets subsequent computational approaches such as principal component analysis plus linear discriminant analysis (PCA-LDA), derived data reduction is achieved to facilitate the visualization of wavenumber-related alterations in target cells. Discriminating spectral variables might be associated with lipid or glycogen content, conformational protein changes and phosphorylation, and structural alterations in DNA/RNA. Using this approach, we investigated the dose-related effects of BaP in MCF-7 cells concentrated in S- or ...
This article presents a novel route for crosslinking a polysaccharide and polysaccharide/protein shell coated on magnetic nanoparticles (MNPs) surface via condensation reaction with squaric acid (SqA). The syntheses of four new types of collagen-, chitosan-, and chitosan-collagen coated magnetic nanoparticles as supports for enzyme immobilization have been done. Structure and morphology of prepared new materials were characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR), XRD, and TEM analysis. Next, the immobilization of lipase from Candida rugosa was performed on the nanoparticles surface via N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC)/N-hydroxy-succinimide (NHS) mechanism. The best results of lipase activity recovery and specific activities were observed for nanoparticles with polymer shell crosslinked via a novel procedure with squaric acid. The specific activity for lipase immobilized on materials crosslinked with SqA (52 U/mg lipase) was about
A Fourier transform infrared spectrometer equipped with a cylindrical internal reflection element was used to sample the solidâ€liquid interface of a metallic copper film submerged in an aqueous acidic polysaccharide solution. The presence of a polysaccharide absorption band at 1050 cmâˆ1 in a water-subtracted spectrum supported previous spectroscopic data indicating polymer accumulation at the surface of the film. Firm binding of the polysaccharide to the surface after a 17-day exposure period was demonstrated by the retention of the 1050-cmâˆ1 absorption band in spectra obtained after gentle rinsing of the film surface with polymer-free water. The sampling technique also provided evidence which suggests that acidic polysaccharides, including the firmly bound exopolymers produced by adherent cells of a freshwater sediment bacterium, promoted deterioration of the copper film. Internal reflection Fourier transform infrared spectroscopy appeared to be a useful, nondestructive method to ...
Oestrogen is essential for the normal functioning of the male and female reproductive systems. Increased oestrogenic activity, commonly due to obesity, is an important cause of pathology in oestrogen-sensitive tissues. Two approaches have been used, firstly, real-time polymerase chain reaction (RT-PCR) with immunohistochemistry and secondly, attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy ...
The intercalation of organic compounds and polymers into common minerals and the chemical analysis of the resulting composite can easily be performed by students in a variety of laboratory courses including materials chemistry, inorganic chemistry, physical chemistry, and instrumental analysis. This exercise introduces students to minerals and their structural framework as well as the utility of these materials to act as effective templates for chemistry. The intercalation of these compounds can serve as models for the preparation of nanocomposite materials where the mineral acts as host to the organic substrate. In this experiment, bentonite (BENT) and kaolinite (KAO) were intercalated with dimethylsulfoxide (DMSO) and poly(ethylene glycol) (PEG-5000). Analyses were performed using differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and diffuse reflectance Fourier transform infrared spectroscopy (DRIFTS). These analyses are used to verify intercalation and determine the ...
We report on the solar-light-mediated degradation of trimethyl phosphate (TMP) and triethyl phosphate (TEP) on hematite and goethite nanoparticles in synthetic air. Adsorption and photoreactions of TMP and TEP were studied by in situ diffuse reflectance Fourier transform infrared spectroscopy (DRIFTS) on dry and water-precovered nanoparticles in dark and under simulated solar light irradiation. Two-dimensional correlation analysis of infrared spectra was used to identify surface products as a function of reaction time. The optical properties of the hematite and goethite nanoparticles were investigated with optical spectrophotometry. The optical band gap was determined by analysis of the Tauc relationship around the band gap energy, E-g, yielding band gap energies of 2.14 and 2.28 eV for hematite and goethite nanoparticles, respectively. It is found that both TMP and TEP are readily photodegraded upon solar light irradiation (employing AM1.5 filters with 1735 W m(-2)), yielding surface ...
Fourier transform infrared (FT-IR) spectra have been measured for defatted bovine serum albumin (BSA) in D,sub,2,/sub,O with a concentration of 2.0 wt % over a temperature range of 26-90 °C and the corresponding difference spectra have been calculated by subtracting the contribution of D,sub,2,/sub,O at the same temperature. Evolving factor analysis (EFA) by selecting two factors and three factors has been employed to analyze the temperature-dependent difference IR spectra in the 1700-1600 cm,sup,-1,/sup, spectral region of the defatted BSA in D,sub,2,/sub,O solution. Three-factor EFA has been employed to determine the distinction of the three protein species involved in the process of temperature elevation: native, transitional, and denatured protein. The temperature profiles obtained from three-factor EFA indicate that heat-induced conformational change in the secondary structures of defatted BSA in D,sub,2,/sub,O undergoes two two-state transitions, a drastic transition and a slight ...
Two types of inulins of different composition were investigated in the glassy and in the crystalline states, at relative humidities within 11 and 97%. The melting and glass transition temperatures (Tm, Tg), and their crystallinity indexes (CI) were determined by modulated differential-scanning calorimetry (MDSC) and wide-angle X-ray scattering (WAXS), respectively. In parallel assays, Fourier transform-infrared spectroscopy (FTIR) coupled to principal component analysis (PCA) enabled a physical-chemical and structural characterization of samples, explaining 90% of the total variance. Finally, partial least square (PLS) models were defined to determine Tg, Tm, and CI directly from the FTIR spectra, using the MDSC and WAXS results as reference methods. In all cases, the mean of predicted values fitted very well those of the reference methods (R2 > 0.961), thus supporting the use of the PLS models to investigate unknown samples. The robustness of the models underlines the usefulness of FTIR to ...
CLX (celecoxib), a selective COX-2 (cyclo-oxygenase-2) inhibitor, has numerous pleiotropic effects on the body that may be independent of its COX-2 inhibitory activity. The cancer chemopreventive ability of CLX, particularly in CRC (colorectal cancer), has been shown in epidemiological studies. Here we have, for the first time, examined the biophysical effects of CLX on the cellular membranes of COX-2 expressing (HT29) and COX-2 non-expressing (SW620) cell lines using ATR-FTIR (attenuated total reflectance-Fourier transform IR) spectroscopy and SL-ESR (spin label-ESR) spectroscopy. Our results show that CLX treatment decreased lipid fluidity in the cancer cell lines irrespective of COX-2 expression status. As metastatic cells have higher membrane fluidity, we examined the effect of CLX on the metastatic potential of these cells. The CLX treatment efficiently decreased the proliferation, anchorage-independent growth, ability to close a scratch wound and migration and invasion of the CRC cell ...
The coking process produces great volumes of wastewater contaminated with pollutants such as cyanides, sulfides and phenolics. Chemical and physical remediation of this wastewater removes the majority of these pollutants; however, these processes do not remove phenol and thiocyanate. The removal of these compounds has been effected during bioremediation with activated sludge containing a complex microbial community. In this investigation we acquired activated sludge from an industrial bioreactor capable of degrading phenol. The sludge was incubated in our laboratory and monitored for its ability to degrade phenol over a 48 h period. Multiple samples were taken across the time-course and analysed by Fourier transform infrared (FT-IR) spectroscopy. FT-IR was used as a whole-organism fingerprinting approach to monitor biochemical changes in the bacterial cells during the degradation of phenol. We also investigated the ability of the activated sludge to degrade phenol following extended periods ...
Fourier transform infrared (FT-IR) spectroscopy is a rapid, reagent-less, non-destructive, analytical technique whose continuing development is resulting in manifold applications in the biosciences....
Dr Fawzi Abou-Chahine is a Spectroscopist at Specac, where he is involved in product testing. In this webinar, he explains the theory behind FT-IR (Fourier transform infrared spectroscopy) and gives examples of its application in a variety of industries. He also covers some of the new technological developments which are modernizing the technique.
TY - JOUR. T1 - Study by Fourier Transform Infrared Spectroscopy of the Adsorption of Carbon Monoxide on a Nickel Electrode at pH 3-14. AU - Cuesta, Angel. AU - Gutierrez, Claudio. PY - 1998. Y1 - 1998. M3 - Article. VL - 14. SP - 3397. EP - 3404. JO - Langmuir. JF - Langmuir. SN - 0743-7463. ER - ...
Diffuse-reflectance IR Fourier transform (DRIFT) spectroscopy was used to study in situ, the low-temp. (T |200 Deg) methanation of CO2 over Ru on TiO2 and on Al2O3 supports. For 3.8% Ru/TiO2, the reaction exhibits an activation energy (Ea) of 19 kcal/mol, is 0.43 +- 0.05 order in H2 concn., and essentially independent of CO2 concn. At 110 Deg, 40% of the available metal sites are occupied by CO (qCO = 0.4), a known methanation intermediate. In contrast to Ru/TiO2, Ru/Al2O3, despite having the same Ea and qCO = 0.2, is 15 times less active. Batch catalyst screening expts. showed no dependence of methanation activity on adsorbed CO(COa) formation rate (as modeled by HCOOH dehydration) or on qCO. In view of this, and the fact that CO dissocn. is structure-sensitive, heterogeneity in the active sites is invoked to reconcile the data. The high Ru dispersion on TiO2 is believed to contribute to the enhanced activity over this support. Adsorbed CO2 and H2 react, possibly at the metal-support interface, to form
Tea polysaccharides have attracted scientific interest due to their antidiabetic effects, and lower quality tea leaves have more polysaccharide in their content compared to higher grade tea leaves. The aim of this study was to optimise the ultrasound-assisted extraction (UE) conditions of polysaccharides from low-grade green tea (GTPS) by Box-Behnken response surface design on the desired response (yield). The optimal extraction parameters were determined as follows: extraction temperature (80 degrees C), extraction time (60 min), ultrasound power (400 W), and liquid to solid ratio (22 ml:g). The experimental yield of GTPS (4.65 +/- 0.29%) obtained under these conditions were well agreed with the value predicted by the model. Without applying ultrasound, while the other extraction conditions were the same (CE), the extraction yield was lower (1.83 +/- 0.04%). Fourier transform-infrared spectroscopy (FT-IR) was used for the identification of functional groups present in GTPS and gel permeation ...
TY - JOUR. T1 - Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells. AU - Heraud, Philip Robert. AU - Ng, Elizabeth Siew Sun. AU - Caine, Sally. AU - Yu, Qing Cissy. AU - Hirst, Claire Elizabeth. AU - Mayberry, Robyn Anne. AU - Bruce, Amanda Jayne. AU - Wood, Bayden Robert. AU - McNaughton, Donald. AU - Stanley, Edouard. AU - Elefanty, Andrew George. PY - 2010. Y1 - 2010. N2 - Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. ...
Predicting the contents of polysaccharides and its monosugars in Dendrobium huoshanense by partial least squares regression model using attenuated total reflectance Fourier transform infrared spectroscopy
Cao J, Ng ES, McNaughton D, Stanley EG, Elefanty AG, Tobin MJ, Heraud P. Fourier transform infrared microspectroscopy reveals that tissue culture conditions affect the macromolecular phenotype of human embryonic stem cells. The Analyst 138 (14) : 4147 - 60(2013) PubMed (PDF ...
Factors limiting hydrogen sulfide production were identified in a two-species biofilm containing sulfate-reducing bacteria (Desulfovibrio desulfuricans) and nonsulfate-reducing bacteria (Pseudomonas fluorescens). Profiles of hydrogen sulfide (H2S) concentration, pH, local mass-transport coefficient, local flow velocity, and local relative effective diffusivity in the biofilm were measured using microelectrodes. Biofilms had a heterogeneous structure consisting of cell clusters separated by voids. Typically, the H2S concentration was lower in the voids than in the adjacent cell clusters, demonstrating that the voids acted as transport channels for removing H2S from cell clusters. The extent of biofilm heterogeneity was directly correlated with the flux of H2S from cell clusters. At flow velocities below 2 cm/s, the flux of H2S from cell clusters depended on the flow velocity. We concluded that at these flow velocities the H2S production rate was limited by the delivery rate of sulfate ions to the ...
TY - JOUR. T1 - Active-Site Glu165 Activation in Triosephosphate Isomerase and Its Deprotonation Kinetics. AU - Deng, Hua. AU - Dyer, R. Brian. AU - Callender, Robert. PY - 2019/5/16. Y1 - 2019/5/16. N2 - Triosephosphate isomerase (TIM) catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde 3-phosphate (GAP) via an enediol(ate) intermediate. The active-site residue Glu165 serves as the catalytic base during catalysis. It abstracts a proton from C1 carbon of DHAP to form the reaction intermediate and donates a proton to C2 carbon of the intermediate to form product GAP. Our difference Fourier transform infrared spectroscopy studies on the yeast TIM (YeTIM)/phosphate complex revealed a C=O stretch band at 1706 cm-1 from the protonated Glu165 carboxyl group at pH 7.5, indicating that the pKa of the catalytic base is increased by ,3.0 pH units upon phosphate binding, and that the Glu165 carboxyl environment in the complex is still hydrophilic in spite of the ...
en] The structure, composition, and physico-chemical properties of lipid-protein complexes generated between dimyristoylphosphatidylcholine (DPMC) and the CNBr fragments of human apoA-I were studied. The fragments were separated by high performance liquid chromatography and purified on a reversed-phase column. The complexes with DMPC were isolated on a Superose column; their dimensions were obtained by gradient gel electrophoresis and by electron microscopy. The secondary structure of the protein in the complexes was studied both by circular dichroism and by attenuated total reflection infrared spectroscopy. The fragments 1 and 4 of apoA-I, containing, respectively, two and three amphipathic helices, recombined with the phospholipid to generate discoidal particles with sizes similar to that of apoA-I- and apoA-II-DMPC complexes. The infrared measurements indicated that in all complexes the apolipoprotein helical segments were oriented parallel to the phospholipid acyl chains and that the protein ...
The aim of this study was to develop a thermally and operationally stable trypsin through covalent immobilization onto chitosan magnetic nanoparticles (Fe3O4 @CTS). The successful preparation of the Fe3O4 @CTS nanoparticles was verified by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and transmission electron microscopy (TEM), which indicated that the prepared Fe3O4 @CTS nanoparticles have superparamagnetic properties, with an average size of approximately 17 nm. Then, trypsin was covalently immobilized onto the Fe3O4 @CTS nanoparticles at a high loading capacity (149.25 mg/g). The FTIR data demonstrated that the trypsin had undergone a conformational change compared with free trypsin, and the Michaelis constant (Km) and the maximum hydrolysis reaction rate (Vmax) showed that the trypsin immobilized on the Fe3O4 @CTS had a lower affinity for BAEE and lower activity compared with free trypsin. However, the immobilized trypsin ...
Abstract : Resorcinol is widely used in manufacturing of several drugs and pharmaceutical products that are mainly usedfor topical ailments. The main objective of this study is to use an alternative strategy i.e., biofield treatment to alterthe physical, spectral and thermal properties of resorcinol. The resorcinol sample was divided in two groups, whichserved as control and treated group. The treated group was given biofield treatment and both groups i.e., control andtreated were analysed using X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, UV-Visible (UVVis)spectroscopy, Differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA). The results showeda significant decrease in crystallite size of treated sample i.e., 104.7 nm as compared to control (139.6 nm). The FTIRand UV-Vis spectra of treated sample did not show any change with respect to control. Besides, thermal analysisdata showed 42% decrease in latent heat of fusion. The onset temperature of ...
Fourier-transform infrared spectroscopy has shown alterations of spectral characteristics of cells and tissues as a result of carcinogenesis. The research reported here focuses on the diagnosis of cancer in formalin-fixed biopsied tissue for which immunochemistry is not possible and when PAP-smear results are to be confirmed. The data from two groups of patients (a control group and a group of patients diagnosed with cervical cancer) were analyzed. It was found that the glucose/phosphate ratio decreases (by 23-49%) and the RNA/DNA ratio increases (by 38-150%) in carcinogenic compared with normal tissue. Fourier-transform microspectroscopy was used to examine these tissues. This type of study in larger populations may help to set standards or classes with which to use treated biopsied tissue to predict the possibility of cancer. Probabilistic neural networks and statistical tests as parts of these biopsies predict the possibility of cancer with a high degree of accuracy (,95%).. © 2005 Optical ...
A Gram-negative bacterium isolated from the sub-surface water of Ikang River, Niger Delta region, Nigeria produced an unusual biosurfactant in waste frying oil-minimal medium. Cultural and biochemical characterizations as well as 16S rRNA sequencing identified the bacterium as a strain of Pseudomonas aeruginosa with 100% sequence homology with Pseudomonas aeruginosa strain HNYM41. Biochemical characterizations, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and Fourier transform-infrared (FT-IR) spectrometry identified the active compound as a glycolipopeptide (peptidoglycolipid) composed of 40.36% carbohydrates, 20.16% proteins and 34.56% lipids. The biosurfactant reduced surface tension of water from 72.00 to 24.62 dynes/cm at a critical micelle concentration (CMC) of 20.80 mg/L indicating excellent effectiveness and efficiency properties. Commendable oil-washing property (79.92% oil recovery) with an elution rate of 0.68 mL/min at 70°C, foaming and foam ...
Article Fourier transform infrared spectroscopic investigation of humic substance samples of different origin and typology supported by chemometric tools. In this paper 198 FTIR spectra of humic substance (HS) extracted from lake, marine sediments, s...
Nanocrystalline CoyZn1-yHozFe2-zO4 (where y = 0.0, 0.25, 0.5, 0.75, 1.00 and z=0.0, 0.03, 0.06, 0.08, 0.1) ferrites were prepared by sol-gel auto combustion method at pH of 8. Samples were obtained by annealing at relatively low temperature 600 °C for 4 h and characterized by thermo gravimetric/differential thermal analysis (TG/DTA) all the samples were annealed at 600 °C for 4 h. The prepared samples were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) spectroscopy. Particle size measured from XRD and TEM are in good agreement with each other. The TEM study reveals the fine particle nature of the ferrites with little agglomerations. The cation distribution suggests that Zn2+ ion mainly on tetrahedral-A sites, Ho3+ ions shows strong preference towards octahedral-B site, Co2+ and Fe3+ ions are randomly distributed at the tetrahedral-A and octahedral-B site. FT-IR study confirmed two main
In this work we have used Fourier transform infrared (FTIR) / vibrational absorption (VA) spectroscopy to study two cancer cell lines: the Henrietta Lacks (HeLa) human cervix carcinoma and 5637 human bladder carcinoma cell lines. Our goal is to experimentally investigate biochemical changes and differences in these cells lines utilizing FTIR spectroscopy. We have used the chemometrical and statistical method principal component analysis (PCA) to investigate the spectral differences. We have been able to identify certain bands in the spectra which are so-called biomarkers for two types of cell lines, three groups for the 5637 human bladder carcinoma cell line (5637A, 5637B and 5637C), and another one for the HeLa human cervix carcinoma cell line. The vibrational modes can be assigned to specific bands involving characteristic motions of the protein backbone. This work shows that infrared vibrational absorption (VA) spectroscopy can be used as a useful tool in medical diagnostics that provides in ...
The differentiation of roots of agricultural species is desired for a deeper understanding of the belowground root interaction which helps to understand the complex interaction in intercropping and crop-weed systems. The roots can be reliably differentiated via Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR). In two replicated greenhouse experiments, six pea cultivars, five oat cultivars as well as seven maize cultivars and five barnyard grass proveniences (n = 10 plants/cultivar or provenience) were grown under controlled conditions. One root of each plant was harvested and five different root segments of each root were separated, dried and measured with FTIR-ATR spectroscopy. The results showed that, firstly, the root spectra of single pea and single oat cultivars as well as single maize and single barnyard grass cultivars/proveniences separated species-specific in cluster analyses. In the majority of cases the species separation was correct, but in a few cases, the
Attenuated total reflection (ATR) infrared absorption spectroscopy is a well-known vibrational spectroscopy technique for many different applications. In recent years this technique has been used to detect thin layer(s) lying on a solid substrate. Su
In this report, a facile, efficient and low cost electrochemical sensor based on bimetallic Au-Cu nanoparticles supported on P nanozeolite modified carbon paste electrode (Au-Cu/NPZ/CPE) was constructed and its efficiency for determination of hydrazine in trace level was studied. For this purpose, agro waste material, stem sweep ash (SSA) was employed as the starting material (silica source) for the synthesis of nano P zeolite (NPZ). After characterization of the synthesized NPZ by analytical instruments (scanning electronic microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy), construction of Au-Cu/NPZ/CPE was performed by three steps procedure involving preparation of nano P zeolite modified carbon paste electrode (NPZ/CPE), introducing Cu +2 ions into nano zeolite structure by ion exchange and electrochemical reduction of Cu +2 ions upon applying constant potential ...
This study was intended to isolate and investigate endophytic fungi and its metabolite as an active biocontrol agent. Endophytic fungal secondary metabolites are considered as an effective and environmentally friendly alternative to synthetic chemicals for integrated pest management. In this study, different parts of medicinal plant Plectranthus amboinicus (Oregano) were selected to isolate twenty ...
The objective of this study was to improve the dissolution rate of a poor water soluble drug, piroxicam, by solid dispersion technique. Solid dispersions were prepared by three different methods depending on the type of carrier. The dissolution rate of piroxicam was markedly increased in solid dispersion of myrj 52, Eudragit® E100 and mannitol. Solubility studies revealed a marked increase in the solubility of piroxicam with an increase in myrj 52 and Eudragit® E100 concentrations. Data from the X-ray diffraction and FT-IR spectroscopy showed that piroxicam was amorphous in the solid dispersions prepared with dextrin and Eudragit® E100.. ...
Variable selection with supervised classification is currently an important tool for discriminating biological samples. In this paper, 15 supervised classification algorithms based on a support vector machine (SVM) were applied to discriminate Cryptococcus neoformans and Cryptococcus gattii fungal species us
A lifetime of 20-30 years is generally regarded as necessary for photovoltaic modules to achieve economic break even. As a consequence, understanding how to improve the durability and reliability of the modules is becoming a necessity. Photovoltaic modules are exposed to extremely harsh conditions of heat, humidity, and ultraviolet (UV) radiation which affect the properties of the encapsulant material and cause yellowing, delamination and degradation of the material, which knock on effects on the performance and the long-term reliability of photovoltaic modules. This study addresses the impact of UV on the photochemical degradation of Ethylene-vinyl Acetate (EVA). Fourier Transform Infrared Spectroscopy in Attenuated Total Reflectance (FTIR-ATR) mode was performed on aged samples. The samples were exposed to UV light from a xenon lamp at 0.68 W/m2 at 340 nm with exposure up to 1000 hours. The FTIR-ATR measurement shows significant changes in the absorption at 1740 cm-1, 1720 cm-1 and 910 cm-1 ...
Hydroxyapatite (HA) having chemical formula Ca10(PO4)6(OH)2, is the main chemical component of human bone tissue (70%). This is the reason why it has been widely engaged in the dental and non-load bearing implantations, to cope up with the bone response as a bioactive material. In this study HA powder was synthesized by wet chemical method, using phosphoric acid (H3PO4) and eggshells. The synthesized HA powder was characterized by X-ray diffraction analysis, Scanning electron microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Fourier transform (FT-IR) spectroscopy. The Thermos gravimetric analysis (TGA-DTA) was also carried out to evaluate the stability of the synthesized HA powder at higher temperatures. The results of the study indicate that sintered (at 900°C) HA powder resembles the feature of pure and single apatite phase having favourable Ca/P ratio ranging from 1.7 to 2.4.
Infrared (IR) absorption spectroscopy directly probes the vibrational modes associate with the molecular bonds in a sample by measuring absorption in the mid-infrared spectral region, ~ 3 - 20 microns. IR spectroscopic measurements are thus intrinsically endowed with a level of chemical specificity and information content far exceeding that of most other optical measurement techniques.[1] Despite their potential, IR absorption measurements suffer not only from limited sensitivity, but are severely hindered by the strong, broad absorption of water that overlaps the bands of most organic compounds of interest. While recent surface enhanced infrared absorption (SEIRA) spectroscopy measurements have shown that IR resonant nanoantennas can be leveraged to dramatically increase sensitivity, [2-3] these have all been performed in dry environments and without time-resolution ...
TY - JOUR. T1 - Quantitative analysis of albumin adsorption onto uncoated and poly(ether)urethane-coated ZnSe surfaces using the attenuated total reflection FTIR technique. AU - Jeon, Joong S.. AU - Raghavan, Srini. AU - Sperline, R. P.. PY - 1994/11/17. Y1 - 1994/11/17. N2 - An attenuated total reflection (ATR) Fourier transform infrared radiation (FTIR) technique has been explored for the in situ quantitative analysis of bovine serum albumin (BSA) adsorption from aqueous solutions onto a segmented poly(ether)urethane film deposited on a ZnSe internal reflection element (IRE) and onto a bare ZnSe IRE surface. The IR spectral area of the amide II band was used for the quantitative analysis of adsorption. BSA adsorption reached equilibrium within 30 min and changed linearly with solution concentration in the range 0.5-6 wt.% and was found to be dependent on the solution pH and substrate type. At the physiological concentration, BSA adsorption densities of 2.4 mg cm-2 and 3.9 μg cm-2 were ...
Environmental protection is worldwide considered a primary issue of the sustainable development. In order to preserve our environment, a major challenge is to remedy the polluted environment by re-using wastes. In this work biowaste sourced soluble substances (CVT230) are used for the design of two types of heterogeneous photocatalysts for wastewater cleaning: an inorganic perovskite and a organic-inorganic hybrid. The materials were carefully characterized by XRD combined with Rietveld refinement, N2 adsorption, FT-IR spectroscopy, thermo gravimetric analysis, transmission electron microscopy and zeta potential analysis. Their photocatalytic activity was tested for the abatement of pollutants found in wastewaters, 4-methylphenol (4-MP), a representative phenolic compound and/or the Crystal Violet (CV), a cationic dye. The experiments were performed in aqueous solution irradiated by simulated solar light ...
The photo-oxidation of propane in dry and humid synthetic air was studied on anatase and rutile nanoparticles by operando diffuse reflectance Fourier-transform infrared (DRIFT) spectroscopy and mass spectrometry (MS). Analysis of the propane removal and CO2 production rate shows that the carbon mass balance (CMB) is CMB , 1 under all reaction conditions up to 1 h operation, and that it is significantly higher on rutile than anatase in humid reaction gas. Corresponding analysis of DRIFT spectra acquired simultaneously as the MS measurements shows that the CMB data scales with the concentration of surface species, i.e. the deviations from unity in the reactant-product mass balance (1-CMB) is quantitatively accounted for by a corresponding increase of surface species measured by DRIFT. The identity of surface species and rate determining reactions steps is deduced by fitting the mode-resolved vibrational IR bands to a kinetic model that accounts for observed temporal evolution of absorption bands. ...
Polymer thin films derived from the essential oil of Lavandula angustifolia (LA) are fabricated using plasma polymerization, and their surface and chemical characteristics investigated. The surface morphology of the polyLA films is examined using an atomic force microscope (AFM). The polymer is found to be uniform and pinhole free, and the average roughness of the films is found to be less than a nanometer and independent of the RF power employed during fabrication. Fourier transform infrared (FTIR) spectroscopy analysis of the polyLA film is performed. Comparison of the FTIR spectra for polyLA film with that of the starting monomer demonstrates that many of the original functional groups are retained during the polymerization process. Bands assigned to C==C stretching are lost due to their participation in the polymerization reactions. With increased RF power employed during fabrication, a decrease in intensity of most of the remaining bands in the FTIR spectra for the polyLA films is found. ...
The aim of the present study was to investigate the Ethanol extract leaf from Jasminum sambac Linn. Qualitative analysis of showed the presence of alkaloids, carbohydrates, flavonoids, tannins, phenol, steroids, terpenoids and glycosides. Absence of leaves extracts protein, mucilage, saponins, fats and fixed oils. This work deals with the phytochemical screening and GC-MS, FT-IR studies of the Ethanol extract. The highest peak area of (40.87%) was obtained by 7-Tetradecenal, (Z) - (C14H26O) at retention time of (18.959) and the lowest peak area of (0.16%) was obtained by Phenol, 3, 5-bis (1,1-dimethylethyl)- (C14H22O) at retention time of (12.294). The FT-IR spectroscope studies shows different characteristic peak values of many functional groups in the extract. FT-IR analysis of leaf extract confirmed the presence of amide, alcohol, phenol, alkane, carboxylic acid, aldehyde, ketone, alkene, primary amine, aromatic esters, alkyl halide, and aliphatic amine compounds. This study summarizing the ...
inproceedings{BUT97278, author=Lucie {Töröková} and František {Krčma}, title=FTIR Analysis of DC Glow Discharge in CH4-N2 Mixtures, annote=The exploration of planetary atmosphere is being advanced by the exciting results of the Cassini-Huygens mission to Saturn and Titan, its most famous moon. The complex chemistry revealed in such atmospheres leading to the synthesis of bigger molecules is providing new insights into our understanding of how life on Earth developed. In our experiments Titans atmosphere is simulated in a glow-discharge formed from a mixture of CH4-N2 gas. Samples of the discharge gas were analysed by Fourier Transform Infrared Spectroscopy. Measurements revealed HCN and NH3 to be the major products of the plasma with traces of C2H2. Carbon monoxide and water were detected as reaction products in methane-nitrogen gas mixture with 1% of carbon dioxide. These molecules could be precursors for other more complicated molecular structures, even some amino acids., ...
The effect of milling on the solid-state transitions of sulfathiazole polymorphs in the absence and presence of solvent and excipients was monitored by X-ray powder diffraction (XRPD), attenuated total reflectance infrared (ATR-IR) and near-infrared (NIR) spectroscopy. Sulfathiazole forms FII-FV undergo a transformation toward the metastable FI, which involves an intermediate amorphous stage upon milling at ambient temperature. Milling FIII with catalytic amounts of solvent converts FIII to FIV or to mixtures of FI and FIV depending on the solvent used. Pure FIV can be easily prepared from FIII by this method. The physical stability of pure sulfathiazole forms in the presence of different levels of relative humidity (RH) was also investigated. At low RH all sulfathiazole forms are stable but at RH levels above 70% FII, FIII and FIV remain stable while FI and FV transform to mixtures of FII and FIV without any apparent change in the external form of the crystals. Co-milling FIII with... ...
In order to characterize the molecular structure and organization of the lipids, we performed attenuated total reflectance infrared (ATR-IR) spectroscopy measurements. Fig. 3a shows the absorption bands characteristic for the stretching vibrations of the lipid acyl chains for the lipid monolayer (black line) and for the lipid-graphene assembly (red line).28 The presence of these peaks confirms that the lipids remain underneath the graphene. Depending on whether the lipids are in contact or not with graphene, a shift in the peaks maxima is observed, characteristic for changes in the lipid conformation.29 Additionally, a shift was observed in the asymmetric methylene vibration (CH2) from ∼2915 to 2912 cm−1 and in the symmetric methylene vibration (CH2) from ∼2848 to 2844 cm−1, respectively. Furthermore the intensity of the asymmetric and symmetric CH2 bands of the lipid-graphene assembly increased. The observed shift is attributed to a change of the physical properties of the lipids film, ...
In-office and at-home bleaching techniques are widely used methods for the whitening of teeth. However, the safety of these techniques has not been clarified yet. The aim of the current study is to investigate the in-office- and at-home-bleaching-induced structural and quantitative changes in human enamel and dentin at the molecular level, under in vitro conditions. The Fourier transform mid-infrared (mid-FT-IR) spectroscopic technique was used to monitor bleaching-induced structural changes. Band frequency and intensity values of major absorptions such as amide A, amide I, phosphate (PO(4)), and carbonate (CO(3)(-2)) bands, for treatment groups and control, were measured and compared. The results revealed that both procedures have negligible effects on dentin constituents. In office-bleached enamel, in addition to demineralization, a decrease in protein and polysaccharide concentrations, mineral-to-protein ratio, and the strength of hydrogen bonds around NH groups, as well as a change in ...
Abstract: Carbon aerogels were prepared, using a freeze-drying method, from graphene oxide (GO) and carbon nanotube (CNT) hybrid hydrogels. The resulting aerogels were characterized using scanning electron microscopy and Fourier-transformed infrared spectroscopy. The adsorption of U(VI) on the GO-CNT aerogels was studied as a function of solid dosage, pH value, initial concentration, and contact time. The results showed that GO-CNT aerogels have high uranium(VI) removal capacities, and are promising sorbents.. Key words: Graphene/carbon nanotube, Hydrogel, Aerogel, Adsorption, Uranium(VI) ...
We report the FTIR spectra of the Amide I and Amide A vibrational modes of N-methylacetamide (NMA, CH$_3$CONHCH$_3$) isolated in a solid parahydrogen (pH$_2$) matrix. NMA is one of the simplest molecules that contains the peptide bond and has therefore been the subject of numerous studies, especially those concerning peptide structure and dynamics. Solid pH$_2$ is a unique quantum solid matrix host that, among other features, allows for high-resolution IR studies of trapped dopant species. In our preliminary report, talk R008 (2006)} we showed that the Amide I lineshape was surprisingly broad and shifts were observed with small changes in temperature. That talk did not resolve the origin of these shifts with temperature that require many minutes to fully equilibrate. Further investigations have revealed that the frequency and breadth of the transition depends partially on the orthohydrogen concentration in the matrix. The Amide I lineshape displays both reversible and irreversible components ...
FTIR stands for Fourier-transform infrared spectroscopy. Fourier transform is the process of decomposing a signal into the frequencies that combine to make that signal. The process is similar to taking a musical chord and separating it into the individual notes that comprise it. In the case of FTIR, the signal that goes through the Fourier transform process is the sample being exposed to infrared light. Most molecules are infrared active, which means that they react in various ways when exposed to infrared light. FTIR systems can test samples that are in solid, gas, or liquid states.. In the case of cannabis, FTIR systems are used to find optimum ratios and schedules for lighting and watering in order to maximize potency (PDF). An FTIR system can also test strain samples in different physicals states. This allows growers to see the composition of cannabinoids and terpenes in solid and gas form. Many cannabinoids and terpenes have different decarboxylation points, which means that some methods ...
A TG/FTIR instrument that combines thermogravimetric analysis with evolved products analysis by Fourier transform infrared spectroscopy is a powerful thermo-analytical technique providing a quantitative time-resolved analysis of major pyrolysis products of any bio-originated material including tobacco. In this work, we demonstrate how the time-resolved spectra of TG/FTIR along with the target factor analysis (TFA) can be used to identify and then quantify the pyrolysis products.
The phase error caused by sampling the fringe patterns in practical Fourier transform profilometry is discussed. In the fTp method, a grating pattern is projected onto an object surface and the deformed fringe is Fourier-transformed and processed in its spatial frequency domain as well as in its space-signal domain. it has been stated that the fundamental spectrum must separate from zero and higher order spectra, because only the fundamental component is needed for phase retrieval. Here we discuss another kind of spectrum overlapping that is caused by the discrete Fourier transform (DFT). The DFT of a digitized fringe pattern results in periodically equi-spaced frequency islands in the spectrum domain. The higher order spectra from adjacent islands may overlap the fundamental spectrum that is filtered out for phase reconstruction. This kind of overlapping also introduces noise into the reconstructed phase. The conclusion is that to obtain a correct reconstruction of the measured object, the ...
A Fourier-transform infrared spectroscopic comparison of cultured human fibroblast and fibrosarcoma cells: a new method for detection of ...
Plasmonics is the modern field of physics aimed at the interaction between electromagnetic field and free electrons in a metal on metal-dielectric interfaces or in metallic nanoparticles with dielectric surrounding. Our group is oriented mainly on the fabrication of metal nanostructures and structured metal-dielectric interfaces by various methods such as electron beam lithography, focused ion beam, ion beam assisted deposition, selective deposition of metal nanoparticles etc. Moreover, we are able to measure optical properties of the fabricated structures by means of optical dark-field spectroscopy, spectroscopic ellipsometry, scanning near field optical microscopy techniques and fourier transform infrared spectroscopy. The main goal is to utilize the fabricated and characterized plasmonic structures in applications, i.e. plasmon-enhanced photoluminescence, plasmon-enhanced infrared absorption, surface enhanced infrared spectroscopy, enhanced biosensing and optical manipulation below the ...
The Spectrum Two FT-IR Analysis System offers trouble-free analysis of lubricant oil. Modern instrumentation makes it possible to obtain lab-quality results with a portable system making them well suited to on-site analysis.
A simple chemical precipitation technique is reported for the synthesis of a hybrid nanostructure of single-wall carbon nanotubes (SWCNT) and titania (TiO2) nanocrystals of average size 5 nm, which may be useful as a prominent photocatalytic material with improved functionality. The synthesized hybrid structure has been characterized by transmission electron microscopy (HRTEM), energy-dispersive X-ray analysis (EDAX), powder X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. It is clearly revealed that nearly monodispersed titania nanocrystals (anatase phase) of average size 5 nm decorate the surfaces of SWCNT bundles. The UV-vis absorption study shows a blue shift of 16 nm in the absorbance peak position of the composite material compared to the unmodified SWCNTs. The photoluminescence study shows a violet-blue emission in the range of 325-500 nm with a peak emission at 400 nm. The low temperature electrical transport property of the synthesized ...
|p> We have investigated the possibility of avoiding formation of Er-rich oxide clusters in ErAl3O6-TiO2-SiO2 glassy films. Samples containing 0.5, 1 and 3 mol% Er31 were prepared using a precursor with a single, isolated Er-ion, ErAl3(OPri)12, in the metal-organic sol-gel route. The thermal decomposition of the gel films to form amorphous oxide films was studied by thermogravimetry, Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction and by means of a transmission electron microscope, equipped with an energy dispersive spectrometer. The microscopy studies of the oxide films obtained after 2 h at 9008C showed that they were amorphous and free of Er-rich clusters. The optical and vibrational properties of the glasses were studied using FT-IR, Raman scattering and luminescence spectroscopy. The samples exhibit luminescence both in the visible and IR under excitation of the 514.5 and 488 nm Ar1 laser lines. The emission around 1.5 mm was maximum for the 1 mol% sample. The results show
Fourier transform infrared spectroscopy (FTIR) is an important tool to understand the new functional group added or removed ... "Fourier transform infrared (FTIR) spectroscopy". Photosynthesis Research. 101 (2-3): 157-70. doi:10.1007/s11120-009-9439-x. ... X-ray photoelectron spectroscopy (XPS) and X-ray diffractometry (XRD) are widely used to gain insights into film composition ... doi:10.1007/978-1-4684-8610-0_5. ISBN 978-1-4684-8612-4. Jenkins R (July 1974). "X-ray spectroscopy. Leonid Azaroff, McGraw- ...
Fourier-transform infrared spectroscopy Surface plasmon resonance "FT-IR Spectroscopy-Attenuated Total Reflectance (ATR)" (PDF ... ISBN 978-0-470-35250-2. "Fourier Transform Infrared Spectroscopy (FT-IR)". nuance.northwestern.edu. Northwestern University ... of the Dispersion Characteristics of Miniaturized Coiled Reactors with Fiber-Optic Fourier Transform Mid-infrared Spectroscopy ... Jesse Greener, Bardia Abbasi, Eugenia Kumacheva, Attenuated total reflection Fourier transform spectroscopy for on-chip ...
Fourier-Transform Infrared Spectroscopy (FTIR)". The Getty Iris. Retrieved 2019-12-12. "Conservation and Scientific Research". ... method that yields surface topography and texture to analyze surface features Fourier Transform Infrared Spectroscopy (FTIR) ... Ultraviolet radiation and Infrared radiation, in addition to visible light, can be emitted from light sources and can also be ... Light, ultraviolet, and thermometers for infrared radiation are some of the tools used to detect when levels fall outside of an ...
This technique is called Fourier transform infrared spectroscopy. When making transmission measurements, the spectrophotometer ... Additionally, most modern mid-infrared spectrophotometers use a Fourier transform technique to acquire the spectral information ... Atomic absorption spectrophotometry Atomic emission spectroscopy Inductively coupled plasma atomic emission spectroscopy ... "FastTrack™ UV/VIS Spectroscopy" (PDF). www.mt.com. Mettler-Toledo AG, Analytical. 2016. Retrieved Dec 23, 2018. Cortez, C.; ...
Smith, Brian C. (2011). Fundamentals of Fourier Transform Infrared Spectroscopy, Second Edition. CRC Press. p. 177. ISBN ...
Fourier transform infrared spectroscopy P. B. Fellgett (1949). Theory of Infra-Red Sensitivities and its Application to ... Shot noise is the main reason Fourier transform spectroscopy has never been popular for UV and visible light spectrometry. ... Griffiths, Peter R.; James A. De Haseth (2007). "7.4.4 Shot noise". Fourier Transform Infrared Spectrometry. Chemical Analysis ... a multiplexed measurement such as the signal generated by a Fourier transform spectrometer can produce a relative improvement ...
Kawaguchi, Kentarou (1992). "Fourier transform infrared spectroscopy of the BH3 ν3 band". The Journal of Chemical Physics. 96 ( ... Infrared Spectra of Boron Hydride Intermediate Species in Solid Argon". Journal of the American Chemical Society. 116 (11): ... Infrared Spectra of Boron Hydride Intermediate Species in Solid Argon". Journal of the American Chemical Society. 116 (11): ... Atomic Spectroscopy. 65 (5): 360-375. doi:10.1016/j.sab.2010.04.010. Housecroft, C. E.; Sharpe, A. G. (2008). "Chapter 13: The ...
"Infrared Fourier Transform Emission Spectroscopy of CuH and NeH+". Proc. SPIE. Fourier and Computerized Infrared Spectroscopy. ... "Fourier Transform Emission Spectroscopy of the A1Σ+-X1Σ+ Transition of CuD" (PDF). Journal of Molecular Spectroscopy. 139 (2): ... "Fourier transform emission spectroscopy of NeH+". Journal of Molecular Spectroscopy. 113 (2): 451-457. Bibcode:1985JMoSp.113.. ... Bernath, P. F. (2000). "6 Infrared emission spectroscopy" (PDF). Annual Reports on the Progress of Chemistry, Section C. 96 (1 ...
Fourier Transform Infrared Spectroscopy/Attenuated Total Reflectance); and SEM-EDS (Scanning Electron Microscope-Energy ... spectroscopy with CIE L*a*b* measurements and EDXRF (Energy-Dispersive X-Ray Fluorescence) analyses; OM (Optical Microscopy) of ...
MIRAS is for Fourier-transform infrared (FTIR) spectroscopy and microscopy. FTIR is a way to identify the infrared spectrum of ... The beamline has a synchrotron-based infrared spectrometer and microscope capacity covering a wavelength range from around 1 µm ... "Beamline Information - BL01 - MIRAS: Infrared microspectroscopy". CELLS. Retrieved 17 November 2020. "Beamline Information - ...
Fourier transform infrared spectroscopy is used to examine metabolic pathways. In 2020 scientists reported the development of ...
Fuller, Michael P.; Griffiths, Peter R. (1978). "Diffuse reflectance measurements by infrared Fourier transform spectrometry". ... This applies to UV-Vis-NIR spectroscopy or mid-infrared spectroscopy. Diffuser Oren-Nayar reflectance model Reflectivity ... Diffuse reflectance spectroscopy can be used to determine the absorption spectra of powdered samples in cases where ... Kortüm, Gustav (1969). Reflectance spectroscopy Principles, methods, applications. Berlin: Springer. ISBN 9783642880711. OCLC ...
"Fluorescence Lifetime Imaging and Fourier Transform Infrared Spectroscopy of Michelangelo's David". Retrieved March 18, 2016. ...
Fourier transform infrared spectroscopy of dysplastic, papillomavirus-positive cervicovaginal lavage specimens. Gyn Oncol. 1995 ...
"Fourier Transform Infrared Microscopic Imaging of an Embedded Paint Cross-Section". Applied Spectroscopy. 56 (3): 275-283. ... 3.0.co;2-l "A novel method to determine collisional energy transfer efficiency by Fourier transform ion cyclotron resonance ... 3.0.co;2-# "Structural analysis of synthetic homo- and copolyesters by electrospray ionization on a Fourier transform ion ... "Manipulating internal energy of protonated biomolecules in electrospray ionization Fourier transform ion cyclotron resonance ...
As a beamsplitter in Fourier transform infrared spectroscopy, typically with laser applications. Film thicknesses are often in ... including much of the infrared spectrum. For some applications like food packaging, the aluminized boPET film can be laminated ...
TGA can couple with Fourier-transform infrared spectroscopy (FTIR) and mass spectrometry. As the increase of temperature, the ...
"Fourier Transform Emission Spectroscopy of New Infrared Systems of LaH and LaD" (PDF). Journal of Molecular Spectroscopy. 104 ( ... "High-Resolution Fourier Transform Infrared Emission Spectrum of Barium Monofluoride" (PDF). Journal of Molecular Spectroscopy. ... 1996). "High-Resolution Infrared Emission Spectrum of Strontium Monofluoride" (PDF). Journal of Molecular Spectroscopy. 175 (1 ... "Infrared Emission Spectra of BeH and BeD" (PDF). J. Chem. Phys. 118 (3): 1158. Bibcode:2003JChPh.118.1158S. doi:10.1063/ ...
and its adulterants based on fourier-transform infrared spectroscopy (FTIR) and chemometrics". PLOS One. 12 (2): e0172359. doi: ...
Fourier transform infrared (FTIR) spectroscopy has been reported to detect gaseous nerve agents. "Medical Management Guidelines ... Laser photoacoustic spectroscopy (LPAS) is a method that has been used to detect nerve agents in the air. In this method, laser ... CO2 Benzene Formaldehyde Acetaldehyde Ammonia NOx SO2 Ethylene Glycol TATP TNT Non-dispersive infrared techniques have been ... "Optically multiplexed multi-gas detection using quantum cascade laser photoacoustic spectroscopy". Applied Optics. 47 (27): ...
"Authentication of Olive Oil Adulterated with Vegetable Oils Using Fourier Transform Infrared Spectroscopy". LWT - Food Science ... All production begins by transforming the olive fruit into olive paste. This paste is then malaxed to allow the microscopic oil ... Methods employing chromatography/mass spectrometry and spectroscopy are often used to detect adulteration of olive oil These ...
UV Spectroscopy. *Fourier transform infrared spectrometry. See also[edit]. *Murchison meteorite. *Xanthene ...
A fourier transform attenuated total reflection infrared spectroscopy and tryptophan fluorescence quenching analysis" (PDF). ... between the residues in the site of the dimer interface have been verified by cross-linking experiments and EPR spectroscopy ...
Applications of Micro-Fourier Transform Infrared Spectroscopy (FTIR) in the Geological Sciences-A Review. International Journal ... the class of organisms that produced it can often be determined using Fourier transform infrared spectroscopy Chen, Y., Zou, C ...
The Fourier transform infrared (FT-IR) spectroscopy is a non-destructive hermeticity characterization method. The radiation ... The equipment consists of an infrared lamp as light source and an infrared video system (compare to figure "Schematic infrared ... "Infrared spectroscopy as a probe of fundamental processes in microelectronics: silicon wafer cleaning and bonding". Surface ... Infrared (IR) void imaging is possible if the analyzed materials are IR transparent, i.e. silicon. This method gives a rapid ...
The plasticrust samples from both locations were studied using Fourier Transform Infrared Spectroscopy (FTIR) analysis. The ... which vary in thickness and in color and are composed of polyethylene based on Fourier Transform Infrared Spectroscopy (FTIR) ...
"High-Resolution Infrared Fourier Transform Emission Spectroscopy of Metal Hydrides: X2Σ+ state of CaH". Journal of Molecular ... "Fourier transform emission spectroscopy of the E2Π-X2Σ+ transition of CaH and CaD". Journal of Molecular Spectroscopy. 266 (2 ... Gordon, I.; Ram, R. S.; Tereszchuk, K.; Walker, K. A.; Bernath, P. F. (1 April 2011). "Fourier Transform Emission Spectroscopy ... Journal of Molecular Spectroscopy. 212 (1): 17-21. Bibcode:2002JMoSp.212...17P. doi:10.1006/jmsp.2002.8531. Barclay, W. L., Jr ...
Amiot, C. (July 1986). "The Rb2 1 1Πg electronic state by laser-induced fluorescence infrared Fourier transform spectroscopy". ... g electronic state by laser induced fluorescence infrared Fourier transform spectroscopy". Molecular Physics. 61 (1): 51-63. ... There are a few more small absorption features in the near infrared. There is also a dirubidium cation, Rb2+ with different ... Caldwell, C.D.; Engelke, F.; Hage, H. (December 1980). "High resolution spectroscopy in supersonic nozzle beams: The Rb2 B 1Πu- ...
The presence of ammonium was confirmed using the structure refinement and Fourier-transform infrared spectroscopy. X-ray powder ... A quantitative analysis was performed using energy dispersive spectroscopy due to the small sample size of 100 µm smeared on a ...
FTIR): Fourier transform infrared spectroscopy is a technique used to obtain an infrared spectrum of absorption or emission of ... Vibrational spectroscopy covers a range of techniques, including conventional Fourier transform infrared (FTIR), Raman, and ... Inspection of fibers can include Fourier transform infrared (FTIR), Raman, and attenuated total reflectance (ATR) spectroscopy ... and Fourier transform infrared spectroscopy (FTIR)." (ATR): attenuated total reflectance is a sampling technique used in ...
As a beamsplitter in Fourier transform infrared spectroscopy, typically with laser applications. Film thicknesses are often in ... including much of the infrared spectrum. For some applications like food packaging, the aluminized boPET film can be laminated ...
Atomic absorption spectroscopy. Fourier transform infrared spectroscopy. Ultraviolet-visible spectroscopy. Interferometry. ... Fundamental Spectroscopy. Raman spectroscopy. Rayleigh scattering. Fluorescence. Phosphorescence. Jablonski diagram. Emission ... Applied Spectroscopy Techniques. Inductively coupled plasma atomic emission spectroscopy. ... Infrared multiphoton dissociation. Chapter 6. NMR. Nuclear magnetic resonance. Magic angle spinning. Two-dimensional nuclear ...
Fourier transform NIR instruments using an interferometer are also common, especially for wavelengths above ~1000 nm. Depending ... Near-infrared spectroscopy (NIRS) is a spectroscopic method that uses the near-infrared region of the electromagnetic spectrum ... Astronomical spectroscopyEdit. Near-infrared spectroscopy is in astronomy for studying the atmospheres of cool stars where ... "Wireless near-infrared spectroscopy of skeletal muscle oxygenation and hemodynamics during exercise and ischemia". Spectroscopy ...
UV-Vis absorption Spectroscopy, Fourier transform infra-red (FTIR), and Photoluminescence spectrometry. In addition, it is ... energy dispersive X-ray spectroscopy (EDS), X-ray fluorescence (XRF), differential scanning calorimetry (DSC), ...
Fourier-transform infrared spectroscopy (GC-FTIR), lan diode-array UV-VIS (HPLC-UV-VIS).[5] ...
... infrared, visible, ultraviolet, X-rays and gamma rays. Arbitrary electromagnetic waves can be expressed by Fourier analysis in ... Spectroscopy (for example) determines what chemical elements comprise a particular star. Spectroscopy is also used in the ... Any type of electromagnetic energy can be transformed into thermal energy in interaction with matter. Thus, any electromagnetic ... Infrared[edit]. Main article: Infrared. Like radio and microwave, infrared (IR) also is reflected by metals (and also most EMR ...
Electron phenomenological spectroscopy. *EPR spectroscopy. *Force spectroscopy. *Fourier transform spectroscopy. *Glow- ... infrared, radio) but having (in general) higher photon energy due to their shorter wavelength. Because of this, the energy of ... X-ray spectroscopy. Works cited[edit]. *Gilmore G, Hemingway J. Practical Gamma-Ray Spectrometry. John Wiley & Sons, Chichester ... Gamma-ray spectroscopy is the quantitative study of the energy spectra of gamma-ray sources, in such as the nuclear industry, ...
Comisarow MB, Marshall AG (1974). "Fourier transform ion cyclotron resonance spectroscopy". Chemical Physics Letters. 25 (2): ... infrared multiphoton dissociation (IRMPD), blackbody infrared radiative dissociation (BIRD), electron-detachment dissociation ( ... Fourier transform ion cyclotron resonance. Fourier transform mass spectrometry (FTMS), or more precisely Fourier transform ion ... Orbitrap instruments are similar to Fourier transform ion cyclotron resonance mass spectrometers (see text below). Ions are ...
By looking at the wiggles of an interferogram using a Fourier-Transform-Spectrometer, enhanced sensitivity could be obtained in ... and hot so that it emits intense infrared radiation; images have then been made in the infrared, where the planet is brighter ... Emission spectroscopy,[131] phase-resolved[132]. *Speckle imaging[133] / Lucky imaging[134] to detect companion stars that the ... The infrared Spitzer Space Telescope has been used to detect transits of extrasolar planets, as well as occultations of the ...
Kawaguchi, Kentarou (1992). "Fourier transform infrared spectroscopy of the BH3 ν3 band". The Journal of Chemical Physics. 96 ( ... Infrared Spectra of Boron Hydride Intermediate Species in Solid Argon". Journal of the American Chemical Society. 116 (11): ... Infrared Spectra of Boron Hydride Intermediate Species in Solid Argon". Journal of the American Chemical Society. 116 (11): ... Atomic Spectroscopy. 65 (5): 360-375. doi:10.1016/j.sab.2010.04.010.. ...
... using the inverse Fourier transform to create a new timecourse for the voxel. A high-pass filter removes the lower frequencies ... The fMRI procedure can also be combined with near-infrared spectroscopy (NIRS) to have supplementary information about both ... and this plot is created with the Fourier transform technique. Temporal filtering amounts to removing the periodic waves not of ... "Simultaneous measurements of cerebral oxygenation changes during brain activation by near-infrared spectroscopy and functional ...
Fourier transform infrared, and nuclear magnetic resonance spectroscopy. These models are associated with electrophysiological ... Colloidal QD emission can be modulated from UV-Vis to the infrared by using different types of coating agents, such as ZnS, CdS ...
... which involves autocorrelating and discrete Fourier transforming the incoming signal, recovers both the spatial and frequency ... so while the equipment is similar to that used in optical spectroscopy, satellites are required to record much of the infrared ... Astronomical spectroscopy is the study of astronomy using the techniques of spectroscopy to measure the spectrum of ... Optical spectroscopyEdit. Incident light reflects at the same angle (black lines), but a small portion of the light is ...
Infrared light transmission photometry[edit]. Main article: Infrared spectroscopy. Spectrophotometry in infrared light is ... The interference pattern can be analyzed using a Fourier transform algorithm. In this way, the whole wavelength range can be ... Therefore, infrared spectroscopy is either performed in the gaseous phase (for volatile substances) or with the substances ... Raman spectroscopy. *Photodetector - A transducer capable of accepting an optical signal and producing an electrical signal ...
"Fourier transform emission spectroscopy of the g4Δ-a4Δ system of FeCl" (PDF). Journal of Molecular Spectroscopy. 221 (2): 261. ... has been detected by infrared spectroscopy at 4 K after cocondensation of laser-ablated Fe atoms with a mixture of O2/Ar.[48] ... Reiff, William Michael; Long, Gary J. (1984). "Mössbauer Spectroscopy and the Coordination Chemistry of Iron". Mössbauer ... O transforms γ-olivine into a mixture of silicate perovskite and ferropericlase and vice versa. In the literature, this mineral ...
... atomic absorption spectroscopy(AAS), Fourier transform infrared spectroscopy (FTIR), এবং thin layer chromatography(TLC) ইত্যাদি ...
Spectroscopy techniques, including Fourier-transform infrared spectroscopy, Raman spectroscopy, and nuclear magnetic resonance ... are known to yield very narrow linewidths which is useful for spectroscopy and analytical applications.[52] An important ... spectroscopy, can be used to determine the chemical composition.. *Differential scanning calorimetry is used to characterize ...
A Fourier transform is carried out to extract the frequency-domain spectrum from the raw time-domain FID. A spectrum from a ... Thermal infrared spectroscopy. *Video spectroscopy. *Vibrational circular dichroism. *Vibrational spectroscopy of linear ... Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a ... Other types of two-dimensional NMR include J-spectroscopy, exchange spectroscopy (EXSY), Nuclear Overhauser effect spectroscopy ...
Fourier transform infrared (FTIR) spectroscopy is a measurement technique that allows one to record infrared spectra. Infrared ... Infrared spectroscopy (IR spectroscopy or vibrational spectroscopy) involves the interaction of infrared radiation with matter ... a b Chromatography/Fourier transform infrared spectroscopy and its applications, by Robert White, p7 ... Nonlinear two-dimensional infrared spectroscopy[18][19] is the infrared version of correlation spectroscopy. Nonlinear two- ...
"Fourier Transform Spectroscopy of the Electronic Transition of the Jet-Cooled CCI Free Radical" (PDF). Retrieved 2007-12-06.. ... Harris, D. C. (1999). Materials for infrared windows and domes: properties and performance. SPIE Press. pp. 303-334. ISBN 0- ... "Fourier Transform Spectroscopy of the System of CP" (PDF). Retrieved 2007-12-06.. ... 44]) under normal conditions (298 K, 105 Pa) and transform into graphite.[18] Due to a high activation energy barrier, the ...
"3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography". Nature Methods. 10 (9): 861-864. doi: ... Photoacoustic spectroscopy TAT Ultrasound-modulated optical tomography Ultrasound UOT Ultrasound computer tomography Ultrasound ... Infrared microtomographic imaging[8]. Mid-infrared 2013 Laser Ablation Tomography Laser Ablation & Fluorescent Microscopy LAT ...
Smiechowski, M.; Stangret, J. (2006). "Proton hydration in aqueous solution: Fourier transform infrared studies of HDO spectra ... as later confirmed by NMR spectroscopy, and not P(OH)3 which would be expected to have a pKa near 8.[44] ... but it has been confirmed by Raman spectroscopy that this is due to the hydrolysis equilibrium:[12] ...
Figure 8. Fourier transform spectroscopy Figure 9. A picture of the solar corona taken with the LASCO C1 coronagraph ... far infrared laser, infrared laser, or visible laser). Each measurement of a single spectral line required several years of ... In Fourier transform spectroscopy they are used to analyze light containing features of absorption or emission associated with ... "Fourier-transform spectroscopy using holographic imaging without computing and with stationary interferometers" (PDF). Physics ...
The term Fourier-transform infrared spectroscopy originates from the fact that a Fourier transform (a mathematical process) is ... Fourier-transform infrared spectroscopy (FTIR)[1] is a technique used to obtain an infrared spectrum of absorption or emission ... Fourier transform infrared spectroscopy is used in geology, chemistry, materials and biology research fields. ... Near-infrared[edit]. Main article: Near-infrared spectroscopy. The near-infrared region spans the wavelength range between the ...
Infrared spectroscopy. Deuterium oxide is often used instead of water when collecting FTIR spectra of proteins in solution. H2O ... Katz, J.; Crespy, H. L. (1972). "Biologically important isotope hybrid compounds in nmr: 1H Fourier transform nmr at unnatural ... از آن در ساخت دستگاه های NMR spectroscopy استفاده میشود که -- -- یکی از موارد کاربرد آن کشف و نمایش شکل ملکول های مواد دارویی ... Semi-heavy water could, in theory, be created via a chemical method, but it would rapidly transform into a dynamic mixture of ...
... atomic emission spectroscopy, ultraviolet-visible spectroscopy, x-ray fluorescence spectroscopy, infrared spectroscopy, Raman ... Fourier transform ion cyclotron resonance, and so on. Electrochemical analysis[edit]. Further information: Electroanalytical ... infrared spectroscopy, liquid chromatography-mass spectrometry, liquid chromatography-NMR spectroscopy. liquid chromagraphy- ... nuclear magnetic resonance spectroscopy, photoemission spectroscopy, Mössbauer spectroscopy and so on. ...
Syberg, F. Suveyzdis, Y., Kotting, C., Gerwert K., & Hofmann, E. Time-Resolved Fourier Transform Infrared Spectroscopy of the ...
Functional near-infrared spectroscopyEdit. Main article: Functional near-infrared spectroscopy. FNIR Is a relatively new non- ... Nemirovsky J, Shimron E (2015). "Utilizing Bochners Theorem for Constrained Evaluation of Missing Fourier Data". arXiv: ... transformed, or adapted. A work consisting of editorial revisions, annotations, elaborations, or other modifications which, as ... NIRS (near infrared spectroscopy) is used for the purpose of functional neuroimaging and has been widely accepted as a brain ...
The term Fourier-transform infrared spectroscopy originates from the fact that a Fourier transform (a mathematical process) is ... Fourier-transform infrared spectroscopy (FTIR)[1] is a technique used to obtain an infrared spectrum of absorption or emission ... Fourier transform infrared spectroscopy is used in geology, chemistry, materials and biology research fields. ... Near-infrared[edit]. Main article: Near-infrared spectroscopy. The near-infrared region spans the wavelength range between the ...
Diffuse reflectance infrared fourier transform spectroscopy (DRIFTS) is an infrared spectroscopy sampling technique used on ... Fuller, Michael P.; Griffiths, Peter R. (1978). "Diffuse reflectance measurements by infrared Fourier transform spectrometry". ... so this would infer that it should be less than 5 µm for mid-infrared spectroscopy. The spectra are plotted in units of log ... The infrared light on a sample is reflected and transmitted at different amounts depending on the bulk properties of the ...
Journal of Spectroscopy is a peer-reviewed, Open Access journal that publishes original research articles as well as review ... articles on the theory and application of spectroscopy across all disciplines. Articles may contribute to fundamental research ... Fourier transform-infrared (FT-IR) spectroscopy has become an important tool for rapid analysis of complex biological samples. ... Fourier transform infrared spectroscopy of dental unit water line biofilm bacteria. Iram Liaqat1,2 ...
Infrared spectroscopy can and has played a part in the characterisation of biological calcium phosphates. Fourier transform ... magnesium phosphates at pH7 and characterization by X-ray absorption and Fourier transform infrared spectroscopy. J. Cryst. ... Ishitari, A., Ishida, H., Soeda, F. and Nagasawa, Y. (1982). Fourier transform infrared reflection spectroscopy for chemical ... Bailey R.T., Holt C. (1989) Fourier transform infrared spectroscopy and characterisation of biological calcium phosphates. In: ...
Cancer cells Research Cancer treatment Chemical properties Health aspects Structure Fourier transform infrared spectroscopy ... Curcumin-DNA interaction studied by Fourier Transform Infrared spectroscopy.(deoxyribonucleic acid, Report) by International ... Transform+Infrared...-a0215925286. *APA style: Curcumin-DNA interaction studied by Fourier Transform Infrared spectroscopy.. (n ... Fourier transform infrared spectroscopy for the forensic identification of fibers.. Vibrational spectroscopy of biological and ...
... Bjørn Petter ... G. Kos, H. Lohninger, and R. Krska, "Fourier transform mid-infrared spectroscopy with attenuated total reflection (FT-IR/ATR) ... "Differentiation and detection of microorganisms using fourier transform infrared photoacoustic spectroscopy," Journal of ... and a hardwood decayed by a brown-rot fungus with the acetyl bromide method and Fourier transform infrared spectroscopy," ...
... difference spectroscopy, microwave radiation was found to affect the bacteriorhodopsin (BR) structure in films at a 30% ... Abstract-Using optical and Fourier transform infrared (FTIR) ... P. R. Griffiths and J. A. de Haseth, Fourier Transform Infrared ... Abstract-Using optical and Fourier transform infrared (FTIR) difference spectroscopy, microwave radiation was found to affect ... Studies by Optical and Fourier Transform Infrared Difference Spectroscopy. ...
Recent studies have explored the utility of Fourier transform infrared spectroscopy (FTIR) in dynamic monitoring of soluble ... between Soluble and Membrane-Embedded Potassium Channel Peptides Monitored by Fourier Transform Infrared Spectroscopy. Table 2 ...
Buy the Paperback Book Fourier Transform Infrared Spectroscopy by T. Theophanides at Indigo.ca, Canadas largest bookstore. + ... Fourier Transform Infrared Spectroscopy was the occasion of the 29th Annual Conference of the Spectroscopy Society of Canada. ... Fourier Transform Infrared Spectroscopy: Industrial Chemical and Biochemical Applications. EditorT. Theophanides. Paperback , ... Title:Fourier Transform Infrared Spectroscopy: Industrial Chemical and Biochemical ApplicationsFormat:PaperbackPublished: ...
Fourier transform infrared (FTIR) spectroscopic techniques are valued for their rapid, nondestructive analysis of materials as ... Fourier transform infrared (FTIR) spectroscopic techniques are valued for their rapid, nondestructive analysis of materials as ... Infrared Chemical Imaging: The Future of FourierTransform IR Spectroscopy. Photonics Spectra. Dec 2004 Over the past two ... Figure 1. Managing, visualizing and analyzing the data generated in Fourier transform infrared chemical imaging requires a ...
... spectroscopy was evaluated as an alternative method for serotyping encapsulated Streptococcus pneumonia strains. Sixty-nine ... Fourier-transform infrared (FTIR) spectroscopy was evaluated as an alternative method for serotyping encapsulated Streptococcus ... Serotype Discrimination of Encapsulated Streptococcus Pneumoniae Strains by Fourier-transform Infrared Spectroscopy and ... FTIR spectroscopy proved to be able to correctly predict 100% of strains according to the serogroups (9, 14, 19, 23). A ...
FTIR spectroscopy) covers a wide range of chemical applications, especially for polymers and organic compounds. ... webinars and white papers covering a range of applications for Fourier Transform infrared spectroscopy, Near-infrared ... Spectroscopy, Elemental & Isotope Analysis Resource Library. Access a targeted collection of application notes, case studies, ... Thermo Scientific Knowledge Base for Molecular Spectroscopy offers a web-based, self-help option for answering product ...
In this study, Fourier transform infrared spectra were collected from 88 endoscopic biopsy colorectal tissue samples (41 ... Combining Fourier transform infrared spectroscopy (FTIR) with endoscopy, it is expected that noninvasive, rapid detection of ... Combining Fourier transform infrared spectroscopy (FTIR) with endoscopy, it is expected that noninvasive, rapid detection of ... Keywords: Fourier transform infrared spectroscopy (FTIR); colorectal cancer; pattern recognition; entropy weight local- ...
Direct-Reading Fourier Transform Infrared Spectroscopy for Occupational and Environmental Air Monitoring ... It provides general guidance for the use of direct-reading Fourier Transform Infrared (FTIR) Spectroscopy for occupational and ...
Optical Analysis of Glioma: Fourier-Transform Infrared Spectroscopy Reveals the IDH1 Mutation Status. Ortrud Uckermann, Tareq A ... Fourier-Transform Infrared Spectroscopy Reveals the IDH1 Mutation Status. Ortrud Uckermann, Tareq A. Juratli, Roberta Galli, ... Fourier-Transform Infrared Spectroscopy Reveals the IDH1 Mutation Status. Ortrud Uckermann, Tareq A. Juratli, Roberta Galli, ... Optical Analysis of Glioma: Fourier-Transform Infrared Spectroscopy Reveals the IDH1 Mutation Status ...
... spectroscopy is a rapid, reagent-less, non-destructive, analytical technique whose continuing development is resulting in ... Fourier transform infrared spectroscopy of follicular fluids from large and small antral follicles. Hum Reprod 15: 1667-1671 ( ... Fourier transform infrared (FT-IR) spectroscopy is a rapid, reagent-less, non-destructive, analytical technique whose ... Perromat A, Melin AM, Deleris G. Pharmacologic application of Fourier transform infrared spectroscopy: the in vivo toxic effect ...
Study of Cancer Cell Lines with Fourier Transform Infrared (FTIR)/ Vibrational Absorption (VA) Spectroscopy. Author(s): Enrique ... In this work we have used Fourier transform infrared (FTIR) / vibrational absorption (VA) spectroscopy to study two cancer cell ... Abstract:In this work we have used Fourier transform infrared (FTIR) / vibrational absorption (VA) spectroscopy to study two ... Title:Study of Cancer Cell Lines with Fourier Transform Infrared (FTIR)/ Vibrational Absorption (VA) Spectroscopy ...
The coupling of a Focal Plane Array (FPA) infrared microscope system to a synchrotron light source at IRENI enables rapid ... malaria infected cells at the single cell level using focal plane array Fourier transform infrared imaging spectroscopy B. R. ... malaria infected cells at the single cell level using focal plane array Fourier transform infrared imaging spectroscopy ... Brighter laboratory based infrared sources, optical refinements and higher sensitive detectors will soon see the emergence of ...
Use of Fourier transform infrared spectroscopy (FTIR) spectroscopy for rapid and accurate identification of yeasts isolated ... Fundamentals of Fourier transform infrared spectroscopy, 2nd ed. Taylor and Francis Group, Boca Raton, FL. ... Influence of cell parameters in Fourier transform infrared spectroscopy analysis of whole yeast cells. Analyst 136:2339-2349. ... Fourier transform infrared (FTIR) spectroscopy is a well-established analytical technology that has been used in various fields ...
Heim by Fourier transform infrared spectroscopy Author(s): Gang Liu; Dingshan Song; Jian-hong Liu; Dezhang Zhao; Yilan Zhou; ... In this paper, Fourier transform infrared speciroseopy (FTIR) was used to characterize the fruiting bodies of the two wild ... The results show that each mushroom has its characteristic infrared spectrum, in which the major peaks are attributed to ...
Fourier transform infrared (FT-IR) spectroscopy and imaging of the nucleus to characterize DNA contributions in different ... Infrared (IR) spectroscopy has the potential to measure differences between normal and cancerous cells. When analyzing biopsy ... sections using IR spectroscopy, careful analyses become important since biochemical variations may be misinterpreted due to ... proteins are important in determining if the cells are actively dividing but no studies on this aspect using IR spectroscopy ...
Whether youre using Fourier-transform infrared spectroscopy to identify organic, polymeric or inorganic materials, we develop ... You can use a single, reliable provider to perform your FTIR spectroscopy maintenance, calibration or qualification. Our ...
Monitoring cellular responses upon fatty acid exposure by Fourier transform infrared spectroscopy and Raman spectroscopy. ... Monitoring cellular responses upon fatty acid exposure by Fourier transform infrared spectroscopy and Raman spectroscopy ... Monitoring cellular responses upon fatty acid exposure by Fourier transform infrared spectroscopy and Raman spectroscopy ...
... ammonia chemical ionization gas chromatography-mass spectrometry and gas chromatography-Fourier transform infrared spectroscopy ... Thiocyanates and isothiocyanates can also be differentiated on the basis of their gas-phase Fourier transform (FT) IR spectra. ... 2165 cm-1. Due to their weak interaction with infrared, aromatic thiocyanates are difficult to detect by this technique. Allyl ...
The IUCr is a scientific union serving the interests of crystallographers and other scientists employing crystallographic methods ...
Infrared emission of radio-frequency-excited (N2 + SiH4) discharge by high-resolution Fourier transform spectroscopy Author(s ... SPIE 1575, 8th Intl Conf on Fourier Transform Spectroscopy, (1 March 1992); doi: 10.1117/12.56353. Show Author Affiliations. ... 8th Intl Conf on Fourier Transform Spectroscopy. Herbert Michael Heise; Ernst Heiner Korte; Heinz W. Siesler, Editor(s) ... Infrared emission of a gas mixture of N2 and SiH4 activated by radio frequency discharge has been analyzed using high ...
... spectroscopy is used to measure the functional group composition of organic aerosols (Russell et al., 2011). However, certain ... functional groups are not directly detected by FTIR spectroscopy, either due to fundamental limitations of the technique (e.g. ... Fourier Transform Infrared (FTIR) spectroscopy is used to measure the functional group composition of organic aerosols (Russell ... Title Prediction Of Organic Aerosol Volatility And Unidentified Functional Group Concentrations From Fourier Transform Infrared ...
The use of Fouriertransform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic ... The use of Fouriertransform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic ... The use of Fouriertransform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic ...
Molecular View by Fourier Transform Infrared Spectroscopy of the Relationship between Lactocin 705 and Membranes: Speculations ... Molecular View by Fourier Transform Infrared Spectroscopy of the Relationship between Lactocin 705 and Membranes: Speculations ... Molecular View by Fourier Transform Infrared Spectroscopy of the Relationship between Lactocin 705 and Membranes: Speculations ... Molecular View by Fourier Transform Infrared Spectroscopy of the Relationship between Lactocin 705 and Membranes: Speculations ...
  • Fourier-transform infrared spectroscopy ( FTIR ) [1] is a technique used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. (wikipedia.org)
  • The goal of any absorption spectroscopy (FTIR, ultraviolet-visible ("UV-Vis") spectroscopy , etc.) is to measure how well a sample absorbs light at each wavelength. (wikipedia.org)
  • The goal of absorption spectroscopy techniques (FTIR, ultraviolet-visible ("UV-Vis") spectroscopy, etc.) is to measure how much light a sample absorbs at each wavelength. (wikipedia.org)
  • Digilab pioneered the world's first commercial FTIR spectrometer (Model FTS-14) in 1969 (Digilab FTIRs are now a part of Agilent technologies's molecular product line after it acquired spectroscopy business from Varian). (wikipedia.org)
  • In a Michelson interferometer adapted for FTIR, light from the polychromatic infrared source, approximately a black-body radiator, is collimated and directed to a beam splitter. (wikipedia.org)
  • Material characterization may be carried out by the attenuated total reflectance (ATR) Fourier transform infrared (FTIR) radiation spectroscopical technique, which represents a powerful experimental tool. (hindawi.com)
  • Thus ATR saves time and enables the study of materials in a pristine condition, that is, the comprehensive sample preparation by pressing thin KBr pellets in traditional FTIR transmittance spectroscopy is hence avoided. (hindawi.com)
  • In this work, we are trying to utilize the attenuated total reflectance (ATR) Fourier transform infrared (FTIR) radiation experimental technique in order to detect wood rot decay and mould fungi growth on building material substrates. (hindawi.com)
  • That is, the extensive, time-consuming, and often cumbersome sample preparation by pressing thin KBr pellets as in traditional FTIR transmittance spectroscopy, which might even change the sample material in question, is avoided. (hindawi.com)
  • For fast and accurate FTIR, FT-NIR, and FT-MIR analysis in a flexible, highly configurable system, count on our market-leading infrared spectrometers and instrumentation. (perkinelmer.com)
  • Abstract -Using optical and Fourier transform infrared (FTIR) difference spectroscopy, microwave radiation was found to affect the bacteriorhodopsin (BR) structure in films at a 30% relative humidity. (springer.com)
  • Fourier transform infrared (FTIR) spectroscopic techniques are valued for their rapid, nondestructive analysis of materials as well as for their ability to identify the chemical signature of small samples. (photonics.com)
  • One of the major barriers to this approach is long acquisition time using Fourier-transform infrared (FTIR) spectroscopy. (rsc.org)
  • Fourier-transform infrared (FTIR) spectroscopy was evaluated as an alternative method for serotyping encapsulated Streptococcus pneumonia strains. (nih.gov)
  • FTIR spectroscopy proved to be able to correctly predict 100% of strains according to the serogroups (9, 14, 19, 23). (nih.gov)
  • Along the way, you can find information on sample preparation for transmission experiments, the power of FTIR Microscopy and charts relating chemical structure to the infrared spectrum. (thermofisher.com)
  • Combining Fourier transform infrared spectroscopy (FTIR) with endoscopy, it is expected that noninvasive, rapid detection of colorectal cancer can be performed in vivo in the future. (mdpi.com)
  • It provides general guidance for the use of direct-reading Fourier Transform Infrared (FTIR) Spectroscopy for occupational and environmental air monitoring. (acgih.org)
  • FTIR spectroscopy can provide the flexibility, sensitivity, and specificity for real-time field measurement of known and unknown components in mixtures. (acgih.org)
  • In this work we have used Fourier transform infrared (FTIR) / vibrational absorption (VA) spectroscopy to study two cancer cell lines: the Henrietta Lacks (HeLa) human cervix carcinoma and 5637 human bladder carcinoma cell lines. (eurekaselect.com)
  • Our goal is to experimentally investigate biochemical changes and differences in these cells lines utilizing FTIR spectroscopy. (eurekaselect.com)
  • The aim of this study was to evaluate Fourier transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) as rapid methods for typing clinical Klebsiella isolates in comparison to whole-genome sequencing (WGS), which was considered the gold standard for typing and identification. (asm.org)
  • Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy is a promising method for rapid and accurate identification of microorganisms. (asm.org)
  • ATR-FTIR spectroscopy is a standalone, inexpensive, reagent-free technique that provides results within minutes after initial culture. (asm.org)
  • ATR-FTIR spectroscopy attained comparable identification performance with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In a preliminary validation of the ATR-FTIR method, correct identification rates of 100% and 95.6% at the genus and species levels, respectively, were achieved, with 3.5% unidentified and 0.9% misidentified. (asm.org)
  • Thus, ATR-FTIR spectroscopy provides a new standalone method that can rival MALDI-TOF MS for the accurate identification of a broad range of medically important yeasts. (asm.org)
  • The simplicity of the ATR-FTIR spectroscopy workflow favors its use in clinical laboratories for timely and low-cost identification of life-threatening yeast strains for appropriate treatment. (asm.org)
  • Fourier transform infrared (FTIR) spectroscopy is a well-established analytical technology that has been used in various fields of study, including chemistry, forensics, ecology, medicine, astronomy, and more ( 12 - 14 ). (asm.org)
  • In this paper, Fourier transform infrared speciroseopy (FTIR) was used to characterize the fruiting bodies of the two wild growing edible mushrooms. (spie.org)
  • You can use a single, reliable provider to perform your FTIR spectroscopy maintenance, calibration or qualification. (transcat.com)
  • Fourier-transform infrared (FTIR) imaging is one of the fastest and most versatile screening techniques. (scribd.com)
  • Fourier Transform Infrared (FTIR) spectroscopy is used to measure the functional group composition of organic aerosols (Russell et al. (epfl.ch)
  • 2011). However, certain functional groups are not directly detected by FTIR spectroscopy, either due to fundamental limitations of the technique (e.g. tertiary carbons), or specific limitations of commonly employed methods of sample collection and analysis (e.g. ester and ether groups, Takahama et al. (epfl.ch)
  • Formalin-fixed paraffin-embedded tissue blocks of normal brain and different brain tumours were de-waxed, mounted on low-E slides and desiccated before being analyzed using attenuated total reflection Fourier-transform IR (ATR-FTIR) and Raman spectroscopy. (lancs.ac.uk)
  • ATR-FTIR spectroscopy showed a clear segregation between normal and different tumour subtypes. (lancs.ac.uk)
  • The photoreaction of this mutant pigment was studied by Fourier-transform infrared (FTIR) difference spectroscopy. (hzdr.de)
  • Fourier Transform Infrared (FTIR) spectroscopy is a label free methodology showing promise in characterizing different types of cell death. (up.ac.za)
  • FTIR spectroscopy can be developed into an invaluable tool for the assessment of specific types of chemically induced cell death with notably different molecular signatures depending on whether the cells are cancerous and mechanism of cell death. (up.ac.za)
  • The Fourier transform IR spectrometer (FTIR) involves much simpler instrumentation but more complicated data processing. (chromatography-online.org)
  • Here a Fourier Transform Infrared Spectroscopy (FTIR) mass-balance method is demonstrated for calculating levels of contamination in cleaned sediment core diatom samples from Lake Baikal, Russia. (scribd.com)
  • The laboratory has a specialization in FTIR difference spectroscopy, time-resolved FTIR, low temperature FTIR, and an expertise in the investigation of biochemical reactions and photo-induced reactions. (frisbi.eu)
  • 1. Mezzetti A., Leibl W. (2008), Proton and electron transfer in wild-type and mutant reaction centers from Rhodobacter sphaeroides followed by rapid-scan FTIR spectroscopy. (frisbi.eu)
  • 2. Mezzetti A., Blanchet L., de Juan A., Leibl W., Ruckebusch C. (2010), Ubiquinol formation in isolated photosynthetic reaction centers monitored by time-resolved differential FTIR in combination with 2D correlation spectroscopy and multivariate curve resolution. (frisbi.eu)
  • Turina P., Francia F., Mezzetti A., Leibl W., Venturoli G. (2015), Dehydration affects the electronic structure of the primary electron donor in bacterial photosynthetic reaction centers: evidence from optical and light-induced difference FTIR spectroscopy. (frisbi.eu)
  • Fourier transform infrared spectroscopy (FTIR) is an optical analytical technique frequently used to identify organic and polymeric compounds in solid, liquid and gaseous samples. (infinitalab.com)
  • FTIR uses infrared light to irradiate a sample and polymeric and organic compounds, present in the sample, produce their distinctive fingerprint in the form of an infrared absorption spectrum (interferograms). (infinitalab.com)
  • The FTIR software applies fourier transformations to convert this interferogram to IR spectra that are unique to the various functional groups and chemical bonds present in the sample. (infinitalab.com)
  • Where Can I Do Fourier Transform Infrared Spectroscopy (FTIR) Testing Of Materials? (infinitalab.com)
  • Our network of material testing labs regularly provides fourier transform infrared spectroscopy (FTIR) testing services to identify and characterize materials. (infinitalab.com)
  • OBJECTIVE: The aim of this study was to determine whether potentized homeopathic drugs and their diluent media differ from each other with respect to their Fourier transform infrared (FTIR) spectra. (isharonline.org)
  • This study analyses six different type of polyphenolic extracts by Fourier transform infrared spectroscopy (FTIR) combined with multivariate analysis. (isharonline.org)
  • Fourier transform infrared (FTIR) and resonance Raman (RR) spectroscopies have been employed to investigate the reductive cleavage of the O-O bond of the endoperoxide moiety of the antimalarial drug artemisinin and its analog trioxane alcohol by hemin dimer. (isharonline.org)
  • The FTIR measures the infrared absorptions for chemical compounds, and the data from this instrument can be used to help identify what functional groups, i.e. alcohols and carboxylic acids, are present in a particular molecule. (liberty.edu)
  • Bruker FTIR instruments are versatile analytical tools equipped with options for characterization of a variety of samples in the mid-infrared region. (uwlax.edu)
  • Fourier transform infrared spectroscopy (FTIR) spectra of the PHAs indicated strong characteristic bands at 1282, 1723, and 2934 cm -1 for scl-PHA and at 2933 and 2976 cm -1 for scl-co-mcl-PHA polymer. (elsevier.com)
  • Within the wide range of hyphenated techniques, the coupling of GC to Fourier Transform Infrared Spectroscopy (FTIR) provides a powerful identification tool, also allowing discriminating between isobars and isomers. (spectra-analysis.com)
  • Our study will investigate, how effective differentiation of Fourier transform infrared (FTIR) spectra can be done by FTIR processing techniques and chemometrics. (rtu.lv)
  • FTIR spectroscopy is an important tool in the analysis of calcium phosphate. (rtu.lv)
  • A major advantage of FTIR spectroscopy is, that spectra can be obtained from almost any physical state of sample (solutions, suspensions, powders). (rtu.lv)
  • Abstract The authors measured IR transmission spectra of two different concentrations of bacillus subtilis spores by using Fourier transform infrared spectroscopy (FTIR) technology. (gpxygpfx.com)
  • FTIR Spectral Characteristics of Rice Plant Growing in Mercury Contaminated Soil [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2081-2085. (gpxygpfx.com)
  • Discrimination of Common Wild Mushrooms by FTIR and Two-Dimensional Correlation Infrared Spectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2113-2122. (gpxygpfx.com)
  • Study on Composite Activating Mechanism of Alkali Steel Slag Cementations Materials by XRD and FTIR [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2302-2306. (gpxygpfx.com)
  • Pressure jump relaxation investigations of lipid membranes using FTIR spectroscopy. (ebscohost.com)
  • The relaxation kinetics of aqueous lipid dispersions after a pressure jump (p-jump) were investigated using time-resolved FTIR spectroscopy. (ebscohost.com)
  • Molecular spectroscopic detection techniques, such as Fourier transform infrared spectroscopy (FTIR), provides additional specificity for isomers where often mass spectrometry (MS) fails, due to similar fragmentation patterns. (monash.edu)
  • Fourier transform infrared spectroscopy (FTIR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole cells are analyzed. (kribb.re.kr)
  • Fourier Transform Infrared (FTIR) data on twelve cases of ovarian cancer with different grades of malignancy from patients of different age groups were analyzed. (biomedcentral.com)
  • In this study, we investigated light-induced conformational changes of a cyanobacterium Cry/Phr-like protein (SCry-DASH) with UV-visible and Fourier transform infrared (FTIR) spectroscopy. (scripps.edu)
  • Fourier Transform Infrared (FTIR) spectroscopy can provide quantitative and online real time analysis. (environmental-expert.com)
  • Besides, scanning electron microscope (SEM), polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy-energy spectrum (SEM-EDX) were used to analysis the effect of Cu 2+ on the morphological structure of biofilm. (iwaponline.com)
  • Over time, additional testing methods such as mass spectroscopy, chiral analysis, FTIR Scan, carbon isotope analysis and others have been developed to more accurately identify each individual essential oil constituent. (doterra.com)
  • The nanoparticles were evaluated for particle size, entrapment efficiency, surface morphology, X-ray diffraction (XRD), Fourier transform spectroscopy (FTIR), and differential scanning calorimetry (DSC). (pubmedcentralcanada.ca)
  • The objective of this research is to assess the presence of trace amount of hazelnut oil in olive oil (less than 10%) by Single Base Extension (SBE) and Fourier Transform InfraRed Spectroscopy (FTIR). (ijbiotech.com)
  • Fourier -Transform InfraRed technique has been used for FTIR spectrum comparisons of pure olive oil and hazelnut-adulterated one as well. (ijbiotech.com)
  • In addition to the established KBr pellet technique, attenuated total reflectance (ATR) spectroscopy was used to analyse the spectra of planktonic growth (−80°C liquid cultures) and biofilm growth of six isolates. (hindawi.com)
  • ATR spectroscopy of −80°C planktonic and biofilm growth showed variation in absorption spectra in fingerprint (1200−900 cm −1 ) region. (hindawi.com)
  • Least-squares curve-fitting of Fourier transform infrared spectra with applications to polymer systems. (springer.com)
  • In this study, Fourier transform infrared spectra were collected from 88 endoscopic biopsy colorectal tissue samples (41 colitis and 47 cancers). (mdpi.com)
  • The infrared spectra of proteins, as a prominent example, exhibit strong amide I absorption bands at 1653 cm -1 associated with characteristic stretching of C=O and C-N and bending of the N-H bond (Stuart, 1997). (springer.com)
  • A novel diagnostic test for arthritis: multivariate analysis of infrared spectra of synovial fluid. (springer.com)
  • Multivariate classification of the infrared spectra of cell and tissue samples. (springer.com)
  • Thiocyanates and isothiocyanates can also be differentiated on the basis of their gas-phase Fourier transform (FT) IR spectra. (gc.ca)
  • Principal component analysis (PCA) was used to identify the main sources of variation in the Fourier transform infrared (FT-IR) spectra of 329 wines of various styles. (sun.ac.za)
  • Evaluation of the information content in infrared spectra for protein secondary structure determination. (ac.be)
  • Results from these chemometric analyses showed that infrared spectra contained information allowing for the discrimination and quantification between the three naturally occurring sugars in orange juice to disguise adulteration via dilution. (manchester.ac.uk)
  • Spectra Analysis Instruments, Inc. is a leading supplier of molecular spectroscopy systems and applications for chromatography. (spectra-analysis.com)
  • The IR spectra of thin amorphous films of mixed oxides In2O3â€"SnO2 system have been studied in the spectral range 4000â€"400 cm-1 by Fourier transform infrared technique. (ebscohost.com)
  • The polarized infrared spectra of the monolayer of natural and isotopic CO adsorbed on NaCl(100) were measured and calculated. (ebscohost.com)
  • The differences between the infrared spectra of wet and dried bacteria, as well as free versus attached bacteria, are also discussed. (qxmd.com)
  • The spectra obtained using reflectance micro-FT-IR spectroscopy were comparable to those obtained using other FT-IR techniques. (qxmd.com)
  • In the present study infrared spectra of normal and malignant ovarian tissues were recorded in the 650 cm -1 to 4000 cm -1 region. (biomedcentral.com)
  • Seven infrared bands in the "fast" kinetic spectra were found based on the following three requirements: (1) they are present in the "fast" phases of N131V and D124N mutants, (2) they have reciprocal counterparts in the "slow" kinetic spectra in these mutants, and (3) they are absent in "fast" kinetic spectra of the E278Q mutant. (edu.kz)
  • ENGLISH ABSTRACT: This study shows Fourier transform near infrared (FT-NIR) spectroscopy's application on wine, fruit and dried fruit for quantitative determinations or as a discriminative method for classification purposes. (sun.ac.za)
  • Diffuse reflectance infrared fourier transform spectroscopy (DRIFTS) is an infrared spectroscopy sampling technique used on powder samples without prior preparation. (wikipedia.org)
  • Specific binding of a biotinylated metallocarbonyl-labelled dendrimer to immobilized avidin detected by diffuse-reflectance infrared Fourier transform spectroscopy. (docme.ru)
  • DOI:10.1002/aoc.591 Chemistry Specific binding of a biotinylated, metallocarbonyllabelled dendrimer to immobilized avidin detected by diffuse-reflectance infrared Fourier transform spectroscopy Bogna Rudolf1 , Janusz Zakrzewski1 *, Grzegorz Celichowski2 , Miche?le Salmain3 , Anne Vessie?res3 ** and Ge?rard Jaouen3 1 University of ?o?dz? (docme.ru)
  • Accepted 24 November 2003 Molecular recognition between avidin covalently immobilized at the surface of acrylic resin beads and a transition metallocarbonyl tracer of the biotin ligand was detected using diffuse reflectance infrared Fourier transform spectroscopy. (docme.ru)
  • Access a targeted collection of application notes, case studies, videos, webinars and white papers covering a range of applications for Fourier Transform infrared spectroscopy, Near-infrared spectroscopy, Raman spectroscopy, Nuclear Magnetic Resonance, Ultraviolet-Visible (UV-Vis) spectrophotometry, X-Ray Fluorescence, and more. (thermofisher.com)
  • We set out to determine whether infrared (IR) and/or Raman spectroscopy combined with multivariate analysis could be applied to discriminate between normal brain tissue and different tumour types (meningioma, glioma and brain metastasis) based on the unique spectral "fingerprints" of their biochemical composition. (lancs.ac.uk)
  • Discrimination of tumour classes was also apparent with Raman spectroscopy. (lancs.ac.uk)
  • The current study indicates that IR and/or Raman spectroscopy have the potential to provide a novel diagnostic approach in the accurate diagnosis of brain tumours and have potential for application in intra-operative diagnosis. (lancs.ac.uk)
  • This article focuses on the use of portable and handheld Raman spectroscopy in the field of forensic science and illustrates it with real-world examples. (environmental-expert.com)
  • SAFIRE-A is a new far-infraredFourier Transform (FT) spectrometer which has beenspecifically designed to operate on board of a highaltitude aircraft in the lower stratosphere and uppertroposphere regions where relatively few remotesensing measurements have been made. (lancs.ac.uk)
  • The Fourier Transform IR Spectrometer. (chromatography-online.org)
  • Although individual facilities and research groups may have their own equipment, the Department also maintains a core facility of more than 20 scientific instruments in the areas of Atomic Spectroscopy, Molecular Spectroscopy, Chromatography and Mass Spectrometry, Electrochemistry, and Materials Characterization. (wcu.edu)
  • Fourier transform near infrared spectroscopy can be used on wine as a rapid method to measure the °Brix (residual sugars) content of must and to discriminate between different must samples in terms of their FAN values. (sun.ac.za)
  • Fourier transform near infrared spectroscopy was applied as an alternative non-destructive method for the quantitative determination of the total soluble solid (TSS) content of whole fresh peaches. (sun.ac.za)
  • Fourier transform near infrared spectroscopy was used as a rapid, analytical technique to determine whether the S0₂ and moisture contents of sultanas are within specifications upon arrival at the factory and during processing. (sun.ac.za)
  • Natural history specimen analysis using micro FT-IR attenuated total reflectance spectroscopy and transmission electron microscopy. (qxmd.com)
  • Hence the combination of vibrational spectroscopic simulations and experimental vibrational absorption spectroscopy and imaging are advocated for future developments in this field. (eurekaselect.com)
  • The XAFS station uses a tunable, monochromatic, and moderately high energy X-ray beam (4.5 keV to 23.5 keV) for X-ray absorption spectroscopy measurements. (nist.gov)
  • The use of Fourier‐transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L. (nofima.no)
  • Reflectance micro-Fourier transform infrared (FT-IR) analysis has been applied to characterize biofilm formation of Aquabacterium commune, a common microorganism present on drinking water distribution systems, onto the increasingly popular pipe material stainless steel EN1.4307. (qxmd.com)
  • The absence of sample preparation, the potential to analyze intact samples, and the ability to characterize opaque and thick samples without the need to transfer the bacterial samples to an infrared transparent medium or produce a pure culture were the main advantages of reflectance micro-FT-IR spectroscopy. (qxmd.com)
  • Measurements in the far infrared needed the development of accurately ruled diffraction gratings to replace the prisms as dispersing elements, since salt crystals are opaque in this region. (wikipedia.org)
  • Fourier transform instruments can do better what was done before and make possible some new types of measurements. (springer.com)
  • This paper describes the results of field measurements of trace gases emitted during hazard reduction burns in Australian temperate forests using open-path Fourier transform infrared spectroscopy. (atmos-chem-phys.net)
  • This goal is accomplished by means of spectroscopy measurements, usually after a separation step, consisting of liquid (LC) or gas (GC) chromatography. (spectra-analysis.com)
  • It is further equipped with a Centaurs infrared microscope for spatially-resolved infrared measurements and a PIKE heated, stainless steel, short-path gas cell for gas-phase infrared measurments. (wcu.edu)
  • Recent research suggests that mid-infrared (IR) spectroscopic imaging may augment histology by providing quantitative molecular information. (rsc.org)
  • V. Infrared spectroscopic studies of myeloid leukemia (ML-1) cells at different phases of the cell cycle. (springer.com)
  • This work shows that infrared vibrational absorption (VA) spectroscopy can be used as a useful tool in medical diagnostics that provides in principle additional information and detail to that which can be obtained/provided from conventional histological studies, and more conventional mass spectroscopic and NMR techniques. (eurekaselect.com)
  • Precision Infrared Spectroscopic Imaging. (ebscohost.com)
  • Fourier transform-infrared spectroscopic methods for microbial ecology: analysis of bacteria, bacteria-polymer mixtures and biofilms. (qxmd.com)
  • Helm D, Labischinski H, Schallehn G, Naumann D. Classification and identification of bacteria by Fourier-transform infrared spectroscopy. (springer.com)
  • Secondary Ion Mass Spectroscopy - SIMS Secondary Ion Mass Spectrometry Secondary Ion Mass Spectroscopy or SIMS is a tool for composition analysis of metals, semiconductors, polymers, biomaterials, minerals, rocks, and ceramics. (infinitalab.com)
  • Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. (ac.be)
  • Chapman, D., Jackson, M. and Haris, P. I. (1989) Investigation of membrane protein structure using Fourier transform infrared spectroscopy. (dmu.ac.uk)
  • Infrared spectroscopy can and has played a part in the characterisation of biological calcium phosphates. (springer.com)
  • The most straightforward way to do this, the "dispersive spectroscopy" technique, is to shine a monochromatic light beam at a sample, measure how much of the light is absorbed, and repeat for each different wavelength. (wikipedia.org)
  • this book, through its breadth of coverage and accompanying extensive lists of references, would be of most benefit to current users of dispersive infrared spectroscopy who seek an appreciation of the increased potential available through ft-ir techniques. (indigo.ca)
  • The term Fourier-transform infrared spectroscopy originates from the fact that a Fourier transform (a mathematical process) is required to convert the raw data into the actual spectrum. (wikipedia.org)
  • The first low-cost spectrophotometer capable of recording an infrared spectrum was the Perkin-Elmer Infracord produced in 1957. (wikipedia.org)
  • The infrared absorbance spectrum could be regarded as a "fingerprint" which is a feature of biochemical substances. (hindawi.com)
  • The infrared spectrum of dicalcium phosphate dihydrate (brushite). (springer.com)
  • Interpretation of carbonate bands in infrared spectrum of dental enamel. (springer.com)
  • The beams then recombine to create an interferogram, which is transformed and processed into a spectrum of infrared frequencies corresponding to the chemical structure of the sample. (photonics.com)
  • The results show that each mushroom has its characteristic infrared spectrum, in which the major peaks are attributed to proteins and polysaccharides. (spie.org)
  • The distortion of the evanescent wave by the sample is measured producing a spectrum which is then subject to a Fourier transform. (rsc.org)
  • We study the physical characteristics of RF-embedded SDNA thin films, using the Fourier transform infrared spectrum to understand the interaction between the RF and DNA molecules, current to evaluate the conductance, absorption to understand the RF binding to the DNA and PL to analyse the energy transfer between the RF and DNA. (royalsocietypublishing.org)
  • Thermo Scientific Knowledge Base for Molecular Spectroscopy offers a web-based, self-help option for answering product questions, accessing documentation and resolving issues. (thermofisher.com)
  • The coupling of a Focal Plane Array (FPA) infrared microscope system to a synchrotron light source at IRENI enables rapid molecular imaging at high spatial resolution. (rsc.org)
  • To obtain molecular insights into the lactocin 705 mechanism of action, Fourier transform infrared spectroscopy was used to investigate the interactions of each peptide (Lac705α and Lac705β) with dipalmitoylphosphatidylcholine liposomal membranes. (asm.org)
  • In the present work, the effects of nilotinib resistance on K562 cells were investigated at the molecular level using Fourier transform infrared (FT-IR) spectroscopy. (iyte.edu.tr)
  • Benedetti E, Bramanti E, Papineschi F, Rossi I. Determination of the relative amount of nucleic acids and proteins in leukemic and normal lymphocytes by means of Fourier transform infrared microspectroscopy. (springer.com)
  • Multivariate calibration for the determination of analytes in urine using mid-infrared attenuated total reflection spectroscopy. (springer.com)
  • Here, we demonstrate Fourier transform infrared (FT-IR) spectroscopy as a rapid, high-throughput and quantitative method for the determination of orange juice adulteration. (manchester.ac.uk)
  • Infrared study of adsorbed molecules on metal surfaces by reflection techniques. (springer.com)
  • The Application of Fourier Transform Infrared Spectroscopy to the Study of Membranes. (indigo.ca)
  • This study investigates the viability of adherent epithelial cells maintained for short periods under mineral oils in order to facilitate live cell spectroscopy using FT-IR with subsequent imaging. (bmj.com)
  • This study describes a computer-based technique for classifying and identifying bacterial samples using Fourier-transform infrared spectroscopy (FT-IR) patterns. (nih.gov)
  • A mutant rhodopsin photoproduct with a protonated Schiff base displays an active-state conformation: a Fourier-transform infrared spectroscopy study. (hzdr.de)
  • Study of hydrogen bonding in liquid crystalline solvent by Fourier transform infrared spectroscopy. (semanticscholar.org)
  • 7. Mezzetti A., Leibl W. (2017), Time-resolved infrared spectroscopy in the study of photosynthetic systems. (frisbi.eu)
  • DI-fusion Fourier transform infrared spectroscopy study of the. (ac.be)
  • Fourier transform infrared spectroscopy study of the secondary structure of the reconstituted Neurospora crassa plasma membrane H(+)-ATPase and of its membrane-associated proteolytic peptides. (ac.be)
  • Aims of this study were to investigation the presence of phytochemical compounds in the methanolic extract of Cressa cretica L. leaves by using GC-MS method and report the functional groups by using FT-IR spectroscopy. (edu.pl)
  • J. Liang, D. Drake, and M. Roselius, "Conformer Analysis Study of 1,2-Diiodotetrafluoroethane by Gas Chromatography/Fourier Transform Infrared Spectroscopy," Appl. (osapublishing.org)
  • In recent years, infrared (IR) spectroscopy has gained attention as a simple and inexpensive method for the biomedical study of several diseases. (biomedcentral.com)
  • The findings of this study demonstrate the possible use of infrared spectroscopy in differentiating normal and malignant ovarian tissues. (biomedcentral.com)
  • Rapid identification of Streptococcus and Enterococcus species using diffuse reflectance-absorbance Fourier transform infrared spectroscopy and artificial neural networks. (springer.com)
  • The Intensities of Infrared Spectral Bands. (indigo.ca)
  • Because of its chemical information content and spectral richness (defined as numbers of clearly defined peaks) the major wavenumber region of interest is the mid-infrared, usually defined as 4000-600 cm -1 (see Table 1). (springer.com)
  • It displayed infrared-difference absorption bands significantly different from those of native MII over a large spectral range. (hzdr.de)
  • Optical Properties Research of Bacillus Subtilis Spores by Fourier Transform Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(12): 3193-3196. (gpxygpfx.com)
  • In geoscience professions, these tools are increasingly being supplemented by field and laboratory instrumentation that utilizes spectroscopy. (environmental-expert.com)
  • Fourier transform-infrared (FT-IR) spectroscopy has become an important tool for rapid analysis of complex biological samples. (hindawi.com)
  • X-Ray Fluorescence Spectroscopy-XRF X-Ray Fluorescence Spectroscopy XRF is an elemental and chemical analysis tool. (infinitalab.com)
  • The effect of dual crosslinking and their successful interaction within the matrix was evaluated by infrared analysis spectroscopy. (mdpi.com)
  • Fourier transform infrared least-squares methods for the quantitative analysis of multicomponent mixtures of airborne vapors of industrial hygiene concern. (cdc.gov)
  • Rapid and quantitative detection of the microbial spoilage of meat by Fourier transform infrared spectroscopy and machine learning. (springer.com)
  • We demonstrate the use of Fourier transform infrared spectroscopy (FTIRS) to make quantitative measures of total organic carbon (TOC), total inorganic carbon (TIC) and biogenic silica (BSi) concentrations in sediment. (lu.se)
  • Rapid and quantitative detection of the microbial spoilage in milk using Fourier transform infrared spectroscopy and chemometrics. (qxmd.com)
  • A type of spectroscopy where infrared light is introduced into a prism at an angle exceeding the critical angle for internal reflection. (rsc.org)
  • Reflectance spectroscopy Principles, methods, applications. (wikipedia.org)
  • Methods The MGH-U1 urothelial or CaCo2 colorectal cancer cell lines were grown on plastic surfaces or mid-range infrared transparent windows. (bmj.com)
  • In this work, probing a proton-loading site of cytochrome c oxidase from Paracoccus denitrificans with time-resolved Fourier transform infrared spectroscopy is presented for the first time. (edu.kz)
  • Detection of dysplastic and neoplastic changes of human cervical tissue via infrared microscopy. (springer.com)
  • 2017. "Colorectal Cancer and Colitis Diagnosis Using Fourier Transform Infrared Spectroscopy and an Improved K-Nearest-Neighbour Classifier. (mdpi.com)
  • Fourier transform infrared (FT-IR) spectroscopy is a rapid, reagent-less, non-destructive, analytical technique whose continuing development is resulting in manifold applications in the biosciences. (springer.com)
  • The analytical method used was fourier transform/infrared spectroscopy (FT-IR). (cdc.gov)
  • For other uses of this kind of technique, see Fourier-transform spectroscopy . (wikipedia.org)
  • Fast Fourier transform technique and its application to Fourier spectroscopy. (springer.com)
  • Due to their weak interaction with infrared, aromatic thiocyanates are difficult to detect by this technique. (gc.ca)
  • Infrared spectrometry is a common technique is a common technique used to identify chemical functional groups. (uwlax.edu)
  • P. R. Griffiths and J. A. de Haseth, Fourier Transform Infrared Spectroscopy (Wiley, New York, 1986). (springer.com)
  • The potential for metabolic fingerprinting via Fourier-transform infrared (FT-IR) spectroscopy to provide a novel approach for the detection of plant biochemical responses to N deposition is examined. (nerc.ac.uk)
  • In Chemical Biological and Industrial Applications of Infrared Spectroscopy (ed. (springer.com)
  • The advent of near infrared (NIR) on-combine sensors gives growers the opportunity to measure the grain protein concentration of wheat (Triticum aestivum L.) during harvest. (environmental-expert.com)
  • Managing, visualizing and analyzing the data generated in Fourier transform infrared chemical imaging requires a sophisticated and interactive software package. (photonics.com)
  • Brighter laboratory based infrared sources, optical refinements and higher sensitive detectors will soon see the emergence of focal plane array imaging in the clinical environment. (rsc.org)
  • Aims Fourier transform infrared (FT-IR) imaging is increasingly being applied to biomedical specimens, but strong IR absorption by water complicates live cell imaging. (bmj.com)
  • This represents an effective means of imaging live cells by IR spectroscopy, and also means that imaging is not necessarily a terminal event. (bmj.com)
  • When analyzing biopsy sections using IR spectroscopy, careful analyses become important since biochemical variations may be misinterpreted due to variations in cell cycle. (spie.org)
  • Infrared emission of a gas mixture of N2 and SiH4 activated by radio frequency discharge has been analyzed using high resolution Fourier transform information. (spie.org)
  • Far-infrared emission spectroscopy has beendemonstrated to be a valuable method for remotesensing of trace species in the stratosphere, with theability to simultaneously detect a number of keychemical species. (lancs.ac.uk)
  • The Fourier Transform Infrared Spectroscopy facility at the CEA Paris-Saclay is located at the Laboratory of Fundamental Mechanisms in Bioenergetics (UMR 9198). (frisbi.eu)
  • Whether you're using Fourier-transform infrared spectroscopy to identify organic, polymeric or inorganic materials, we develop and executive protocols according to industry standards and confirm operational specifications to ensure regulatory compliance. (transcat.com)
  • Characterization and quantitation of aprepitant drug substance polymorphs by attenuated total reflectance fourier transform infrared spectroscopy. (qxmd.com)
  • A Survey and Applications to Absolute and Integrated Infrared Intensities. (indigo.ca)
  • Industrial Applications of Fourier Transform Infrared Spectroscopy. (indigo.ca)
  • Applications of infrared spectroscopy to medical biology. (qxmd.com)
  • The applicability of reflectance micro-Fourier-transform infrared spectroscopy for the detection of synthetic microplastics in marine sediments. (qxmd.com)