That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Chromatography, High Pressure Liquid
A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Dose-Response Relationship, Radiation
Indicators and Reagents
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Chromatography, Thin Layer
Blood Volume Determination
Method for determining the circulating blood volume by introducing a known quantity of foreign substance into the blood and determining its concentration some minutes later when thorough mixing has occurred. From these two values the blood volume can be calculated by dividing the quantity of injected material by its concentration in the blood at the time of uniform mixing. Generally expressed as cubic centimeters or liters per kilogram of body weight.
Flow Injection Analysis
A silver metallic element that exists as a liquid at room temperature. It has the atomic symbol Hg (from hydrargyrum, liquid silver), atomic number 80, and atomic weight 200.59. Mercury is used in many industrial applications and its salts have been employed therapeutically as purgatives, antisyphilitics, disinfectants, and astringents. It can be absorbed through the skin and mucous membranes which leads to MERCURY POISONING. Because of its toxicity, the clinical use of mercury and mercurials is diminishing.
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Abnormal responses to sunlight or artificial light due to extreme reactivity of light-absorbing molecules in tissues. It refers almost exclusively to skin photosensitivity, including sunburn, reactions due to repeated prolonged exposure in the absence of photosensitizing factors, and reactions requiring photosensitizing factors such as photosensitizing agents and certain diseases. With restricted reference to skin tissue, it does not include photosensitivity of the eye to light, as in photophobia or photosensitive epilepsy.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Cerebrospinal Fluid Proteins
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Water Pollutants, Chemical
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Limit of Detection
Nephelometry and Turbidimetry
Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
Electron Spin Resonance Spectroscopy
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Platinum. A heavy, soft, whitish metal, resembling tin, atomic number 78, atomic weight 195.09, symbol Pt. (From Dorland, 28th ed) It is used in manufacturing equipment for laboratory and industrial use. It occurs as a black powder (platinum black) and as a spongy substance (spongy platinum) and may have been known in Pliny's time as "alutiae".
Electron Transport Complex IV
A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.
Carbon monoxide (CO). A poisonous colorless, odorless, tasteless gas. It combines with hemoglobin to form carboxyhemoglobin, which has no oxygen carrying capacity. The resultant oxygen deprivation causes headache, dizziness, decreased pulse and respiratory rates, unconsciousness, and death. (From Merck Index, 11th ed)
The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).
A compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN.
Bleeding into the intracranial or spinal SUBARACHNOID SPACE, most resulting from INTRACRANIAL ANEURYSM rupture. It can occur after traumatic injuries (SUBARACHNOID HEMORRHAGE, TRAUMATIC). Clinical features include HEADACHE; NAUSEA; VOMITING, nuchal rigidity, variable neurological deficits and reduced mental status.
Evaluation Studies as Topic
Spectroscopy, Fourier Transform Infrared
Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.
The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).
Magnetic Resonance Spectroscopy
Mammalian pigment cells that produce MELANINS, pigments found mainly in the EPIDERMIS, but also in the eyes and the hair, by a process called melanogenesis. Coloration can be altered by the number of melanocytes or the amount of pigment produced and stored in the organelles called MELANOSOMES. The large non-mammalian melanin-containing cells are called MELANOPHORES.
A thiazide diuretic often considered the prototypical member of this class. It reduces the reabsorption of electrolytes from the renal tubules. This results in increased excretion of water and electrolytes, including sodium, potassium, chloride, and magnesium. It is used in the treatment of several disorders including edema, hypertension, diabetes insipidus, and hypoparathyroidism.
Stable elementary particles having the smallest known negative charge, present in all elements; also called negatrons. Positively charged electrons are called positrons. The numbers, energies and arrangement of electrons around atomic nuclei determine the chemical identities of elements. Beams of electrons are called CATHODE RAYS.
Drugs, Chinese Herbal
The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A noninvasive technique that uses the differential absorption properties of hemoglobin and myoglobin to evaluate tissue oxygenation and indirectly can measure regional hemodynamics and blood flow. Near-infrared light (NIR) can propagate through tissues and at particular wavelengths is differentially absorbed by oxygenated vs. deoxygenated forms of hemoglobin and myoglobin. Illumination of intact tissue with NIR allows qualitative assessment of changes in the tissue concentration of these molecules. The analysis is also used to determine body composition.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.
A shiny gray element with atomic symbol As, atomic number 33, and atomic weight 75. It occurs throughout the universe, mostly in the form of metallic arsenides. Most forms are toxic. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), arsenic and certain arsenic compounds have been listed as known carcinogens. (From Merck Index, 11th ed)
Sensitivity and Specificity
Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)
Particles consisting of aggregates of molecules held loosely together by secondary bonds. The surface of micelles are usually comprised of amphiphatic compounds that are oriented in a way that minimizes the energy of interaction between the micelle and its environment. Liquids that contain large numbers of suspended micelles are referred to as EMULSIONS.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Calorimetry, Differential Scanning
Cytochrome c Group
Rats, Inbred Strains
A common genetically determined, chronic, inflammatory skin disease characterized by rounded erythematous, dry, scaling patches. The lesions have a predilection for nails, scalp, genitalia, extensor surfaces, and the lumbosacral region. Accelerated epidermopoiesis is considered to be the fundamental pathologic feature in psoriasis.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
Characterisation of copper-binding to the second sub-domain of the Menkes protein ATPase (MNKr2). (1/6255)The Menkes ATPase (MNK) has an essential role in the translocation of copper across cellular membranes. In a complementary manner, the intracellular concentration of copper regulates the activity and cellular location of the ATPase through its six homologous amino-terminal domains. The roles of the six amino-terminal domains in the activation and cellular trafficking processes are unknown. Understanding the role of these domains relies on the development of an understanding of their metal-binding properties and structural properties. The second conserved sub-domain of MNK was over-expressed, purified and its copper-binding properties characterised. Reconstitution studies demonstrate that copper binds to MNKr2 as Cu(I) with a stoichiometry of one copper per domain. This is the first direct evidence of copper-binding to the MNK amino-terminal repeats. Circular dichroism studies suggest that the binding or loss of copper to MNKr2 does not cause substantial changes to the secondary structure of the protein. (+info)
Cloning, overexpression, purification, and physicochemical characterization of a cold shock protein homolog from the hyperthermophilic bacterium Thermotoga maritima. (2/6255)Thermotoga maritima (Tm) expresses a 7 kDa monomeric protein whose 18 N-terminal amino acids show 81% identity to N-terminal sequences of cold shock proteins (Csps) from Bacillus caldolyticus and Bacillus stearothermophilus. There were only trace amounts of the protein in Thermotoga cells grown at 80 degrees C. Therefore, to perform physicochemical experiments, the gene was cloned in Escherichia coli. A DNA probe was produced by PCR from genomic Tm DNA with degenerated primers developed from the known N-terminus of TmCsp and the known C-terminus of CspB from Bacillus subtilis. Southern blot analysis of genomic Tm DNA allowed to produce a partial gene library, which was used as a template for PCRs with gene- and vector-specific primers to identify the complete DNA sequence. As reported for other csp genes, the 5' untranslated region of the mRNA was anomalously long; it contained the putative Shine-Dalgarno sequence. The coding part of the gene contained 198 bp, i.e., 66 amino acids. The sequence showed 61% identity to CspB from B. caldolyticus and high similarity to all other known Csps. Computer-based homology modeling allowed the conclusion that TmCsp represents a beta-barrel similar to CspB from B. subtilis and CspA from E. coli. As indicated by spectroscopic analysis, analytical gel permeation chromatography, and mass spectrometry, overexpression of the recombinant protein yielded authentic TmCsp with a molecular weight of 7,474 Da. This was in agreement with the results of analytical ultracentrifugation confirming the monomeric state of the protein. The temperature-induced equilibrium transition at 87 degrees C exceeds the maximum growth temperature of Tm and represents the maximal Tm-value reported for Csps so far. (+info)
Safety and pharmacokinetics of abacavir (1592U89) following oral administration of escalating single doses in human immunodeficiency virus type 1-infected adults. (3/6255)Abacavir (1592U89) is a nucleoside analog reverse transcriptase inhibitor that has been demonstrated to have selective activity against human immunodeficiency virus (HIV) in vitro and favorable safety profiles in mice and monkeys. A phase I study was conducted to evaluate the safety and pharmacokinetics of abacavir following oral administration of single escalating doses (100, 300, 600, 900, and 1,200 mg) to HIV-infected adults. In this double-blind, placebo-controlled study, subjects with baseline CD4+ cell counts ranging from < 50 to 713 cells per mm3 (median, 315 cells per mm3) were randomly assigned to receive abacavir (n = 12) or placebo (n = 6). The bioavailability of the caplet formulation relative to that of the oral solution was also assessed with the 300-mg dose. Abacavir was well tolerated by all subjects; mild to moderate asthenia, abdominal pain, headache, diarrhea, and dyspepsia were the most frequently reported adverse events, and these were not dose related. No significant clinical or laboratory abnormalities were observed throughout the study. All doses resulted in mean abacavir concentrations in plasma that exceeded the mean 50% inhibitory concentration (IC50) for clinical HIV isolates in vitro (0.07 microgram/ml) for almost 3 h. Abacavir was rapidly absorbed following oral administration, with the time to the peak concentration in plasma occurring at 1.0 to 1.7 h postdosing. Mean maximum concentrations in plasma (Cmax) and the area under the plasma concentration-time curve from time zero to infinity (AUC0-infinity) increased slightly more than proportionally from 100 to 600 mg (from 0.6 to 4.7 micrograms/ml for Cmax; from 1.0 to 15.7 micrograms.h/ml for AUC0-infinity) but increased proportionally from 600 to 1,200 mg (from 4.7 to 9.6 micrograms/ml for Cmax; from 15.7 to 32.8 micrograms.h/ml for AUC0-infinity. The elimination of abacavir from plasma was rapid, with an apparent elimination half-life of 0.9 to 1.7 h. Abacavir was well absorbed, with a relative bioavailability of the caplet formulation of 96% versus that of an oral solution (drug substance in water). In conclusion, this study showed that abacavir is safe and is well tolerated by HIV-infected subjects and demonstrated predictable pharmacokinetic characteristics when it was administered as single oral doses ranging from 100 to 1,200 mg. (+info)
Safety and single-dose pharmacokinetics of abacavir (1592U89) in human immunodeficiency virus type 1-infected children. (4/6255)Abacavir (formerly 1592U89) is a potent 2'-deoxyguanosine analog reverse transcriptase inhibitor that has been demonstrated to have a favorable safety profile in initial clinical trials with adults with human immunodeficiency virus (HIV) type 1 infection. A phase I study was conducted to evaluate the pharmacokinetics and safety of abacavir following the administration of two single oral doses (4 and 8 mg/kg of body weight) to 22 HIV-infected children ages 3 months to 13 years. Plasma was collected for analysis at predose and at 0.5, 1, 1.5, 2, 2.5, 3, 5, and 8 h after the administration of each dose. Plasma abacavir concentrations were determined by high-performance liquid chromatography, and data were analyzed by noncompartmental methods. Abacavir was well tolerated by all subjects. The single abacavir-related adverse event was rash, which occurred in 2 of 22 subjects. After administration of the oral solution, abacavir was rapidly absorbed, with the time to the peak concentration in plasma occurring within 1.5 h postdosing. Pharmacokinetic parameter estimates were comparable among the different age groups for each dose level. The mean maximum concentration in plasma (Cmax) and the mean area under the curve from time zero to infinity (AUC0-infinity) increased by 16 and 45% more than predicted, respectively, as the abacavir dose was doubled from 4 to 8 mg/kg (Cmax increased from 1.69 to 3.94 micrograms/ml, and AUC0-infinity increased from 2.82 to 8.09 micrograms.h/ml). Abacavir was rapidly eliminated, with a mean elimination half-life of 0.98 to 1.13 h. The mean apparent clearance from plasma decreased from 27.35 to 18.88 ml/min/kg as the dose increased. Neither body surface area nor creatinine clearance were correlated with pharmacokinetic estimates at either dose. The extent of exposure to abacavir appears to be slightly lower in children than in adults, with the comparable unit doses being based on body weight. In conclusion, this study showed that abacavir is safe and well tolerated in children when it is administered as a single oral dose of 4 or 8 mg/kg. (+info)
The binding affinity of Ff gene 5 protein depends on the nearest-neighbor composition of the ssDNA substrate. (5/6255)The Ff gene 5 protein (g5p) is considered to be a nonspecific single-stranded DNA binding protein, because it binds cooperatively to and saturates the Ff bacteriophage single-stranded DNA genome and other single-stranded polynucleotides. However, the binding affinity Komega (the intrinsic binding constant times a cooperativity factor) differs by over an order of magnitude for binding to single-stranded polynucleotides such as poly[d(A)] and poly[d(C)]. A polynucleotide that is more stacked, like poly[d(A)], binds more weakly than one that is less stacked, like poly[d(C)]. To test the hypothesis that DNA base stacking, a nearest-neighbor property, is involved in the binding affinity of the Ff g5p for different DNA sequences, Komega values were determined as a function of NaCl concentration for binding to six synthetic sequences 48 nucleotides in length: dA48, dC48, d(AAC)16, d(ACC)16, d(AACC)12, and d(AAACC)9A3. The binding affinities of the protein for these sequences were indeed found to be related to the nearest-neighbor compositions of the sequences, rather than to simple base compositions. That is, the g5p binding site, which is spanned by four nucleotides, discriminates among these sequences on the basis of the relative numbers of nearest neighbors (AA, CC, and AC plus CA) in the sequence. The results support the hypothesis that the extent of base stacking/unstacking of the free, nonbound ssDNA plays an important role in the binding affinity of the Ff gene 5 protein. (+info)
Extraction and analysis of cosmetic active ingredients from an anti-cellulitis transdermal delivery system by high-performance liquid chromatography. (6/6255)A new transdermal delivery system that controls cellulitis is evaluated using reversed-phase high-performance liquid chromatography coupled with photodiode array detection. An extraction procedure and the validation of the analytical method to assay the active excipients from the Centella asiatica plant (asiaticoside, madacessic acid, and asiatic acid) are described. Excellent results ae obtained in terms of linearity, accuracy, and specificity of the analytical method. (+info)
Identification of the major tamoxifen-DNA adducts in rat liver by mass spectroscopy. (7/6255)We present here the first mass spectroscopic (MS) identification of the main tamoxifen-induced DNA adducts in rat liver. The two main adducts were isolated by high-performance liquid chromatography (HPLC) and identified by MS, MS-MS and ultraviolet spectroscopy. Adduct 1 was the N-desmethyltamoxifen-deoxyguanosine adduct in which the alpha-position of the metabolite N-desmethyltamoxifen is linked covalently to the amino group of deoxyguanosine. Adduct 2 was confirmed to be the trans isomer of alpha-(N2-deoxyguanosinyl)tamoxifen, as previously suggested by co-chromatography. (+info)
Conformation and self-association of human recombinant transforming growth factor-beta3 in aqueous solutions. (8/6255)The transforming growth factors-beta (TGF-beta) are important regulatory peptides for cell growth and differentiation with therapeutic potential for wound healing. Among the several TGF-beta isoforms TGF-beta3 has a particularly low solubility at physiological pH and easily forms aggregates. A spectroscopic structural analysis of TGF-beta3 in solution has thus been difficult. In this study, circular dichroism spectroscopy was used to determine the secondary structural elements of TGF-beta3. In addition, the aggregation of TGF-beta3 was investigated systematically as a function of pH and salt concentration using a rapid screening method. Sedimentation equilibrium and sedimentation velocity analysis revealed that TGF-beta3 exists predominantly in two major forms: (i) monomers in solution at low pH and (ii) large precipitating aggregates at physiological pH. Under acidic conditions (pH < 3.8) the protein was not aggregated. At pH approximately 3.9, a monomer right arrow over left arrow dimer equilibrium could be detected that transformed into larger aggregates at pH > 4.1. Aggregation was pronounced in the pH range of 4.3 < pH < 9.8 with the aggregation maximum between pH 6.5 and 8. 5. The aggregation process was accompanied by a structural change of the protein. The CD spectra were characterized by an isodichroic point at 209.5 nm indicating a two-state equilibrium between TGF-beta3 dissolved in solution and aggregated TGF-beta3. Aggregated TGF-beta3 showed a higher beta-sheet content and lower beta-turn and random coil contributions compared with monomeric TGF-beta3. Both the solution structure and the aggregate structure of TGF-beta3 were different from the crystal structure. This was in contrast to TGF-beta2, which showed very similar crystal and solution structures. Under alkaline conditions (pH > 9.8) the turbidity disappeared and a further conformational change was induced. The pH dependence of the TGF-beta3 conformation in solution in the range of 2.3 < pH < 11. 0 was reversible. Aggregation of TGF-beta3 was, furthermore, influenced by the presence of salt. For pH > 3.8 the addition of salt greatly enhanced the tendency to aggregate, even in the very basic domain. Under physiological conditions (pH 7.4, cNaCl = 164 mM) TGF-beta3 has almost the highest tendency to aggregate and will remain in solution only at nanomolar concentrations. (+info)
Vacuum ultraviolet spectroscopy of high-pressure helium microbubbles in metals - University of Huddersfield Repository
Lucas, A.A., Donnelly, S. E., Vigneron, J.P. and Rife, J.C. (1983) Vacuum ultraviolet spectroscopy of high-pressure helium microbubbles in metals. Surface Science, 126 (1-3). pp. 66-79. ISSN 0039-6028 Metadata only available from this repository ...
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15.9: Structure Determination in Conjugated Systems - Ultraviolet Spectroscopy - Chemistry LibreTexts
The ultraviolet (UV) region of the electromagnetic spectrum corresponds to conjugated bond energies. Molecular Orbital Theory describes this bond energy using electron transitions from the HOMO and …
Ultraviolet Absorption Spectrum, Hydrogen Peroxide | USP Technologies
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AN INVESTIGATION OF THE EFFECT OF TEMPERATURE ON THE SCHUMANN-RUNGE ABSORPTION CONTINUUM OF OXYGEN, 1580 TO 1950 A.
The effect of temperature on the Schumann-Runge ultraviolet absorption continuum of oxygen has been investigated, using photoelectric techniques, over a temperature range from 300 to 900 K and in the wavelength interval from 1580 to 1950 A. The monochromator bandwidth was 0.075 A. Absolute absorption cross sections were obtained, and curves are presented showing the variation of the cross section with wavelength for the temperatures 300, 600, and 900 K. (Author)(*ULTRAVIOLET SPECTROSCOPY
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Ganetespib biological activity | Role of NK1 and NK2 receptors in mouse gastric mechanical activity
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Electronic delocalization, charge transfer and hypochromism in the UV absorption spectrum of polyadenine unravelled by...
The characterization of the electronically excited states of DNA strands populated upon solar UV light absorption is essential to unveil light-induced DNA damage and repair processes. We report a comprehensive analysis of the electronic properties of the UV spectrum of single-stranded polyadenine based on th
Investigation of Radiation-Induced Damages in DNA Structure by Circular Dichroism and UV Absorption Spectroscopy pp. 301-320
Authors: (S.V. Paston, O.A. Dommes, A.E. Tarasov, Saint Petersburg State University, Faculty of Physics, St. Petersburg, Russia ...
A study of the IR and UV-Vis absorption cross-sections, photolysis and OH-initiated oxidation of CF3CHO and CF3CH2CHO -...
Infrared and ultraviolet-visible absorption cross-sections, effective quantum yields of photolysis and OH reaction rate coefficients for CFCHO and CFCHCHO are reported. Relative rate measurements at 298(2) K and 1013(10) hPa, give (OH + CFCHO)/(OH + CHCH) = 2.00(13), (OH + CFCHCHO)/(OH + CHCHOH) = 1.21(5) and (OH +
Determination of Copper in Water, Vegetables, Foodstuffs and Pharmaceuticals by Direct and Derivative Spectrophotometry
K. ASHOK RAO*, S. SIVARAMAIAH*, K. SARA SUBUDHI, D. SREEVANI, Y. UMAMAHESWARI and G. V. HARITHA,Determination of Copper in Water, Vegetables, Foodstuffs and Pharmaceuticals by Direct and Derivative Spectrophotometry, Chem Sci Trans.,2012,1(3), pp 590-603 ...
UV-Visible Spectroscopy - Principles and Analysis
UV-visible absorption spectroscopy is one of the most widely used techniques in clinical and chemical laboratories. It is employed in qualitative analysis for the identification of chemicals, however its principal use is for the quantitative determination of various organic and inorganic species in solution. This aspect will be the focus of this section.. The essential theory and principles of UV-visible spectroscopy, as well as its applications and limitations for chemical analysis, will be investigated. There are five tasks you will undertake in this section.. ...
Uv Vis Spectrophotometer Price, Uv Vis Spectrophotometer Price Suppliers and Manufacturers at Alibaba.com
Uv Vis Spectrophotometer Price, Wholesale Various High Quality Uv Vis Spectrophotometer Price Products from Global Uv Vis Spectrophotometer Price Suppliers and Uv Vis Spectrophotometer Price Factory,Importer,Exporter at Alibaba.com.
Analysis of feline corneal sequesters
Fluorescence spectroscopic analysis of sequestra, unaffected corneas and contact lenses (from affected and contralateral/unaffected eyes) showed that lipid and protein were present but did not play an important role in sequestra. Ultraviolet-visible light absorbance spectroscopy revealed a peak at 385 nm in unaffected corneas that was absent in sequestra and the difference was significant (P < 0.0001); this peak may be a characteristic feature of the normal feline cornea. The absorbance spectra displayed a peak at 280 nm in two sequestra suggesting that chromophore groups (e.g. melanin) were present ...
Absorbance - UV Spectroscopy | Physics Forums
Hi guys, please can you kindly help me. I am getting an absorbance of over 6.0Abs for my experiment. However, when I am reading posts they mention that an...
M Aldehydes And Ketones - Aromatic Hydrocarbons
Figures 4.26, 4.27, and 4.28 show typical UV absorption spectra for some simple aldehydes and ketones (Rogers, 1990 Martinez et al., 1992 see also Cronin and
ICMAB - New Sensitive and Selective Chemical Sensors for Ni2+ and Cu2+ Ions: Insights into the Sensing Mechanism through DFT...
The interaction of sensors with these metal ions induced a new absorption band with a hypsochromic shift to the characteristic signal of the free sensors. A theoretical study via time-dependent density functional theory (TD-DFT) was performed. This method has enabled us to reproduce the hypsochromic shift in the maximum UV-vis absorption band and explain the selective sensing of the ions. For all of the systems studied, the absorption band is characterized by a π → π* transition centered in the ligand. Instead of Ni2+ and Cu2+ ions, the transition is set toward the σ* molecular orbital with a strong contribution of the 3dx2-y2 transition (π → 3dx2-y2). These absorptions imply a ligand-to-metal charge transfer (LMCT) mechanism that results in the hypsochromic shift in the absorption band of these systems ...
UV WinLab Software for Uv/Vis | PerkinElmer
Know more about UV WinLab, the instrument control and manipulation software for the latest range of PerkinElmer LAMBDA™ 365, High Performance UV/Vis and UV/Vis/NIR spectrophotometers.
Protein quantitation was carried out by two independent methods:. 1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).. 2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.. Usage: ...
UV VIS Manufacturers and Suppliers - Price - MesuLab
Find uv vis from professional Single Beam UV VIS Spectrophotometer manufacturers and suppliers on labinstrumentsmanufacturer.com. We have a professional laboratory at your service. Enjoy the good price.
Spectral Database Index: IR - Industrial Chemicals, Basic Organic Compounds - Wiley
About this Index: This index lists the names of all compounds found in the IR - Industrial Chemicals, Basic Organic Compounds - Wiley spectral database. Bio-Rad is the world leader in spectral databases. They offer over 2.3 million high-quality IR, Raman, NIR, NMR, MS and UV-Vis spectra. Please visit our website for a complete listing. ...
Spectral Database Index: ATR-IR - Solvents - Bio‑Rad Sadtler
About this Index: This index lists the names of all compounds found in the ATR-IR - Solvents - Bio‑Rad Sadtler spectral database. Bio-Rad is the world leader in spectral databases. They offer over 2.3 million high-quality IR, Raman, NIR, NMR, MS and UV-Vis spectra. Please visit our website for a complete listing. ...
UV Vis Spectrophotometer Micro-Volume - PROAnalytics
The Mettler-Toledo Excellence instruments UV Vis spectrophotometer is the ideal tool for spectroscopic analysis in the life sciences field as well as man...
Spectrophotometers - UV Vis | Micro-volume & Cuvette-based
High performance UV Vis cuvette and specialized micro-volume spectrophotometers. Modular design enables optimization of your application workflows. Superior optical technology enables accurate, reliable measurements within 1 second.
Spectrophotometers - UV Vis | Micro-volume & Cuvette-based
High performance UV Vis cuvette and specialized micro-volume spectrophotometers. Modular design enables optimization of your application workflows. Superior optical technology enables accurate, reliable measurements within 1 second.
NEW) Beckman DU730 UV/Vis Spectrophotometer | For Sale | LabX Ad 501818
Beckman DU730 UV/Vis Spectrophotometer Unused unit, in original box. The unit has been sitting for a few years, though it has never been
using UV absorbance scans to measure homogeneity of proteins
In article ,david-0809991507400001 at mac355.nmus.pwf.cam.ac.uk,, david at cryst.bioc.cam.ac.uk (David) wrote: , Im sorry for not making it clear, but the kind of homogeneity I was , thinking about was with respect to proteins rather than nucleic acids, and , I guess in particular, whether a protein that appears pure on, e.g. a gel , or gel filtration columnm, has higher-order oligomers or aggregates , present. As suggested, good ways to study this include gel filtration and , analytical ultracentrifugation, which we have done for our system, but I , had recently heard the advice that one should always as a first step, look , at the UV280 scan to see if there are aggreagates, which was what prompted , my original question. Remember that absorption of light in mainly due to only 3 aas( trp(~280nm),tyr(~275nm,phe~258nm)). If you have a solution of a monomeric protien in solution or a multimeric protein in solution, the absorption spectra will not reveal it. It will just tell you the ...
Analytical Spectrophotometer UV Vis | Larsa Scientific LLC
LabX UV Vis expands the instrument with a sophisticated graphical editor for spectra evaluation, increased automation capabilities, more workflow options, a method of editing and many more features ...
Monitoring of Synthesized Compounds with a SofTA™ ELSD | SelectScience
The following application note shows the usefulness of the SofTA ELSD compared with a UV detector. As glycine methyl ester does not have UV absorbance, it is difficult to monitor this synthesis reaction with a UV detector.
HPLC traces of SFB (black, absorbance at 254 nm), 1-Col | Open-i
HPLC traces of SFB (black, absorbance at 254 nm), 1-Cold (red, absorbance at 320 nm), 18F-SFB (green, radiochromatograph), 1 (blue, radiochromatograph), and rea
2489 UV/Visible (Uv/Vis) Detector : Waters
A versatile, dual-wavelength absorbance detector that offers simplicity for routine UV-based applications and high sensitivity for low-level impurity analysis.
Simultaneous Determination of Paracetamol, Guaiphenesin and Chlorpheniramine Maleate Using Ultraviolet Spectroscopy in...
Abstract: The method of choice for analysis of drugs in multi-component preparations is chromatographic based technique such as High Performance Liquid Chromatography (HPLC). However, chromatographic method is time consuming and requiring much effort. As a consequence, some simple methods such as UV spectrophotometry are continuously developed, especially in combination with the chemometrics software. The UV-vis spectrophotometry coupled with multivariate calibration of Partial Least Square (PLS) has been developed for quantitative analysis of paracetamol, guaiphenesin and chlopheniramine maleate in the presence of phenylpropanolamine without separation step. The calibration model is prepared by developing a series of sample mixture comprising these drugs in certain proportion. The evaluation of calibration model was based on coefficient of determination (R2) and Root Mean Square Error of Calibration (RMSEC). The result showed that UV spectrophotometry combined with PLS can be used for ...
Ultraviolet resonance Raman study of proline isomerization<...
TY - JOUR. T1 - Ultraviolet resonance Raman study of proline isomerization. AU - Harhay, Gregory P.. AU - Hudson, Bruce S.. PY - 1991/1/1. Y1 - 1991/1/1. N2 - The ultraviolet resonance Raman spectra of aqueous solutions of proline dipeptides obtained with 200- and 218-nm excitation at pH 1.5 and 6.5 are reported. The cis/trans isomer ratio for these dipeptides is known to depend on pH. The amide II′ like mode of the X-Pro peptide (imide) linkage at 1487 cm-1, involving predominantly the motions of the peptide carbon and nitrogen, is the most strongly enhanced band in the 200-250-nm region. We observe that this band does not shift appreciably with the change in isomer ratio associated with the change in pH. The same behavior is observed with the 1467-cm-1 band of aqueous polyproline, which has undergone a salt-induced isomerization. We conclude from these observations that contrary to earlier evidence the position of the amide II′ like band is not diagnostic for the isomeric state of the ...
SORCE » References
Bless, R. C., A. D. Code, and E. T. Fairchild, Ultraviolet photometry from the Orbiting Astronomical Observatory. XXI. Absolute energy distribution of stars in the ultraviolet, Astrophysical Journal 203, 410-416, 1976.. Bohlin, R. C., D. Frimout, and C. F. Lillie, Absolute flux measurements in the rocket ultraviolet, Astronomy and Astrophysics 30, 127-134, 1974.. Bohlin, R. C., A. V. Holm, B. D. Savage, M. A. J. Snijders, and W. M. Sparks, Photometric calibration of the International Ultraviolet Explorer (IUE): Low Dispersion, Astronomy and Astrophysics 85, 1-13, 1980.. Brune, W. H., G. H. Mount, and P. D. Feldman, Vacuum ultraviolet spectrophotometry and effective temperatures of hot stars, Astrophysical Journal 227, 884-899, 1979.. Henry, R. C., A. Weinstein, P. D. Feldman, W. G. Fastie, and H. W. Moos, Low-resolution ultraviolet spectroscopy of several hot stars observed from Apollo 17, Astrophysical Journal 201, 613-623, 1975.. Holberg, J. B., W. T. Forrester, and D. E. Shemansky, Voyager ...
Characterisation of DME-HCCI combustion cycles for formaldehyde and hydroxyl UV-vis absorption<...
TY - JOUR. T1 - Characterisation of DME-HCCI combustion cycles for formaldehyde and hydroxyl UV-vis absorption. AU - Azimov, Ulugbek. AU - Stylianidis, Nearchos. AU - Kawahara, Nobuyuki. AU - Tomita, Eiji. N1 - Publisher Copyright: © 2017 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2017. Y1 - 2017. N2 - We investigated time-resolved ultraviolet-visible (UV-vis) light absorbance to identify the formation behaviour of formaldehyde (HCHO) and hydroxyl (OH) within the wavelength range of 280-400 nm in a homogeneous charge compression ignition (HCCI) engine fuelled with dimethyl ether (DME). The time-resolved HCHO and OH profiles at different initial pressures showed that HCHO absorbance increased in the low-temperature reaction (LTR) and thermal-ignition preparation (TIP) regions and decreased gradually as the combustion approached the high-temperature reaction (HTR) region. At higher intake pressures, HCHO absorbance decreased and OH absorbance increased. The ...
Molar absorptivity / molar extinction coefficients for inorganic solutions | VIPEr
Greetings VIPErs,. I am looking for molar absorptivity (or molar extinction coefficient) data for a handful of inorganic compounds (in solution), as a function of wavelength in the UV/visible spectrum. Can you help?. The compounds are Co(NO3)2, CoCl2, K2CrO7, AuCl3, K2CrO4, NiCl2, CuSO4, and KMnO4.. I have tried the following online resources without success: the CRC Handbook, the NIST Chemical WebBook, the Combined Chemical Dictionary, PubChem, SciFinder, and Google.. I would greatly appreciate your suggestions for resources that may have these data! If the search is to no avail, I will probably resort to tracking down a UV/vis spectrophotometer (and the various chemicals) and taking the measurements myself, but I would hugely appreciate a more reliable source, in addition to not having to do this.. Many thanks! ...
Protocol for spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients | Zenodo
Experimental protocol for the spectrophotometric determination of native and desialylated apo-transferrin molar absorption coefficients. Supplemental information for the manuscript in preparation (published version will be linked here).
Highly sensitive and specific high-performance liquid chromatographic analysis of 7-hydroxywarfarin, a marker for human...
The formation of 7-hydroxywarfarin in incubations of (S)-warfarin with human liver microsomes reflects their cytochrome P-4502C9 activity. This paper describes a rapid high-performance liquid chromatographic method for the determination of 7-hydroxywarfarin with high sensitivity, selectivity, and a …
Improved high-performance liquid chromatographic analysis of terazosin in human plasma. - Nuffield Department of Medicine
A simple, sensitive and reproducible high-performance liquid chromatography (HPLC) method was developed for the determination of terazosin in human plasma. The method involves a one-step single solvent extraction procedure using dichloromethane with a 0.25 ml plasma sample. Recovery values were all greater than 90% over the concentration range 0.25-100 ng/ml. Terazosin was found to adsorb to glass or plastic tubes, but this could be circumvented by using disposable plastic tubes. Also, rinsing the injector port with methanol after each injection helped to prevent any carry-over effect. The internal standard, prazosin, did not exhibit this problem. The method has a quantification limit of 0.25 ng/ml. The within- and between-day coefficient of variation and accuracy values were all less than 7% over the concentration range 0.25-100 ng/ml and hence the method is suitable for use in pharmacokinetic studies of terazosin.
Molar extinction coefficient calculation. | Physics Forums
Homework Statement I need to calculate molar extinction coefficient for Albumin at 280nm. Homework Equations A=ε*c*L ε=A/(c*L) But since...
High-Performance Liquid Chromatographic Analysis of Bilirubin and Biliverdin from Jaundiced Broilers
Japans largest platform for academic e-journals: J-STAGE is a full text database for reviewed academic papers published by Japanese societies
Method and Apparatus for the Optical Determination of Total Organic Carbon in Aqueous Streams - Patent application
0008] Organic compounds also absorb strongly in the UV and this absorption has also been exploited as an analog measurement for TOC. Differential absorbance spectroscopy, a measurement of water monitoring as a function of wavelength has been described previously. See Development of Differential UV Spectroscopy for DBP Monitoring, Korshin, G. V., et al., AWWA Foundation, (2002). Monitoring TOC using UV absorption has focused on wavelengths between 200 and 400 nanometers where strong absorption bands of organic compounds occur. Historically this range also represented that part of the UV spectrum accessible by readily available optical components. However, absorptivity of water in this region of electromagnetic spectrum is a potential problem. Consider the plot of water absorptivity versus electromagnetic wavelength shown in the graph of FIG. 1 (derived from http://www.lsbu.ac.uk/water/vibrat.html#uv). The region of wavelengths between 200 nm and 400 nm is region of particular interest because ...
McPherson to Provide Vacuum Ultraviolet Spectrophotometer to Support the Ural Federal University Institute of Physics and...
Ural Federal University (UrFU) Institute of Physics and Technology in Yekaterinburg, Russia contracted McPherson (Chelmsford, MA USA), a manufacturer of ultraviolet spectrometers, to provide a VUVAS instrument in support of the UrFU scientific development program. The new VUVAS PLUS Spectrophotometer system works from 115nm vacuum ultraviolet to 1800nm infrared. The new system measures transmittance and reflectance at adjustable angles. Plus it delivers tunable vacuum ultraviolet (VUV) e
Sensors | Free Full-Text | Ground Based Ultraviolet Remote Sensing of Volcanic Gas Plumes | HTML
Ultraviolet spectroscopy has been implemented for over thirty years to monitorvolcanic SO2 emissions. These data have provided valuable information concerningunderground magmatic conditions, which have been of utility in eruption forecastingefforts. During the last decade the traditionally used correlation spectrometers have beenupgraded with miniature USB coupled UV spectrometers, opening a series of exciting newempirical possibilities for understanding volcanoes and their impacts upon the atmosphere.Here we review these technological developments, in addition to the scientific insightsthey have precipitated, covering the strengths and current limitations of this approach.
Some studies of the chemical reactivity of β-lactam antibiotics
Rate coefficients for the inactivation of several penicillin in acids and alkalis at 30°C have been determined by means of ultraviolet spectroscopy. The case of benzylpenicillin, though well investigated in the past, has been studied again and, in the light of new experiments, a scheme detailing the proportions of the various degradation products has been proposed. A number of substituted phenylpenicillins have been synthesised, and their reactions with acid have been investigated. Product analysis was carried out by means of TLG and NMR studies, the degradation products (penicilloic, penilloic, penillic and penicillenic acids) having been previously prepared and characterised. From the manner in which the phenyl substituent on the penicillin affects the rate coefficient, it is deduced that the mechanism of inactivation by acids involves a rate-determining intramolecular attack by the side-chain carbonyl group on the protonated β-lactam function. An intermediate oxazolone-thiazolidine ...
Ebooks download from here Download CRC Handbook of Fundamental Spectroscopic Correlation Charts Ebook
The handbook presents useful analysis and assignment of spectra and structural elucidation of organic and organometallic molecules. The correlation charts are compiled from an extensive search of spectroscopic literature and contain current, detailed information that includes new results for many compounds. The handbook includes graphical data charts for nuclear magnetic resonance spectroscopy of the most useful nuclei, as well as infrared and ultraviolet spectrophotometry. Because mass spectrometry data is not best represented graphically, the data are presented in tabular form, where mass spectrometry can be used for analyses and structural determinations in tandem with other techniques. In addition to presenting absorption bands and intensities for a variety of important functional groups and chemical families, the book also discusses instrument calibration, diagnostics, common solvents, fragmentation patterns, several practical conversion tables, and laboratory safety ...
Identification and Determination of Strychnine by Ultraviolet Spectrop by Sidney Kaye and Ebbe C. Hoff
Sidney Kaye and Ebbe C. Hoff, Identification and Determination of Strychnine by Ultraviolet Spectrophotometry, 43 J. Crim. L. & Criminology 246 (1952 ...
RheumaKnowledgy » Creatine Phosphokinase (CPK)
Synonyms: Creatine kinase, CK, CPK.. CPT Code: 82550. Description: CPK is an intracellular enzyme found in high concentrations in skeletal muscle, myocardium, and brain. Damage to these tissues results in elevated serum levels of CK. Three isoforms are used to determine the tissue origin of serum CK: skeletal muscle (MM), myocardium (MB), and brain (BB).. Method: Analysis may be part of a selected automated chemistry profile or may be ordered separately. Serum should be collected in a plain red-top tube. Avoid hemolysis. All determinations of CK levels should be done before invasive diagnostic procedures such as electromyography or muscle biopsy. Ultraviolet spectrophotometry is most common.. Normal Values: Values depend on the method used but generally range from 50 to 200 U/L for males; values for females are 25% lower. Black individuals (males more so than females) may have CK levels above normal values. Such values do not correlate with muscle mass and are not associated with an occult ...
Biology-Online • View topic - Molar Extinction Coefficient
The extinction coefficient is present in the beer-lambert law (A=ECL). However, how would I calculate the extinction coefficient from a graph where concentration is on the x-axis and optical density on the y-axis? Would I have to work out a gradient? And so, where do I go next to work out the extinction coefficient ...
Mr. Shuai Huang Profile
Detection of Hg2+ with high sensitivity is of great significance in the biochemical sensing field. Quantitative of Hg2+ was realized based on the influence of Hg2+ on the UV-vis absorption performance of Au-Pt-Au core-shell nanoraspberry (APA)-rhodamine-6G (R6G) structure. First, APA sol was added into R6G indicator solution and the UV-vis absorption signal intensity of R6G was evidently promoted. The signal intensity monotonously increased as more APA sol was added. However, when HgCl2 solution was introduced, the signal intensity declined. A linear relationship between Hg2+ concentration and signal intensity at 527 nm was revealed, based on which quantitative determination of Hg2+ could be realized. Hg2+ detection sensitivity was measured to be 0.031 a.u./M with a limit of detection of 10-7 M and the response time was 20 s. A high Hg2+ detection selectivity over Cu2+, Na+, Li+, and K+ was demonstrated. Due to its simplicity and high sensitivity, the proposed method could find an extensive ...
Biology-Online • View topic - molar extinction coefficient
As you decrease the concentration the absorbance decreases and they should both become zero together so the intercept should be zero; that means from Y=mx+c for c=0 you only need Y=mx. For A=ecl, l=1cm and so except for its contribution to the units you can ignore it. That leaves you with A=ec, which is in the form Y=mx. As you vary the concentration the absorbance changes. The slope of their linear relationship (in the Beers law range, A,~2) is the extinction coefficient. To lay it out explicitly ...
DETERMINATION OF DNA CONCENTRATION AND PURITY 文献中心 贝斯特试剂
Different methods have been developed to determine or estimate the purity and concentration of DNA, such as UV spectrophotometry (1), diphenylamine assay (1, 2), restriction enzyme digestion and gel electrophoresis. Each procedure has advantages and disadvantages. For example, UV spectrophotometry is quite simple for the determination of DNA concentration and purity. However, it but can not be used to estimate the DNA concentration if the DNA sample is contaminated with RNA and some degraded DNA. Moreover, when the DNA sample is contaminated with some organic materials and/or ions, which will inhibit enzymatic reactions (such as restriction digestion), this method can not give any information about the contamination. In these cases, the restriction digestion and gel electrophoresis method can be used to distinguish DNA, degraded DNA, RNA and different contaminants and estimate the DNA concentration if a standard marker is used ...
Solvents & Reagents , Spectroscopy & GC-HS
Information of Solvents & Reagents , Spectroscopy & GC-HS. 1,2-Dichloroethane & UV Spectroscopy, 99.9%, 1,4-Dioxane & UV Spectroscopy, 99.9%, Acetic Acid , 99.9%, Acetone & UV Spec
You may also wish to search for items by Summrell. 2 matching references were found. Cason, J.; Allinger, N.L.; Summrell, G.; Williams, D.E., Branched-chain Fatty Acids XVII. Synthesis of Two Trimethylalkanoic Acids Certain Relationships of Boiling POint and Index of Refraction to Structure, J. Org. Chem., 1951, 16, 1170-6. [all data] Cason, J.; Summrell, G., Branched-chain Fatty Acids XVIII. Ultraviolet Absorption Spectra of Saturated Branched-chain Acids, J. Org. Chem., 1951, 16, 1177-80. [all data] ...
Vacuum UV Spectroscopy - HORIBA
Over the past 35 years, HORIBA Scientific has developed some of the most innovative Vacuum Ultra Violet (VUV) monochromators for synchrotron centers and the wider scientific community.
Difference between revisions of User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/03 - OpenWetWare
The molar absorptivities of two different molecules, adenosine and inosine were determined in this lab using UV-Vis and Beers law. The changes in UV-Vis spectra will be observed to determine changes in concentration of both adenosine and inosine. In order to do this, the molar absorptivity (ε) of both of these molecules will be known. A calibration curve from the class data will be created. From this data the standard deviation, Confidence Interval (90% and 95% confidence) will be calculated and Grubbs test will be performed to determine the outlier. ...
Flash Photolysis 101<...
Instrumentation is used in the undergraduate chemistry curriculum to help demonstrate the fundamental aspects of chemical reactions and demonstrate how it can be used to determine certain properties of a chemical system. For example, absorbance spectroscopy teaches students about transmission and absorption of radiation by a compound and how these measurements can be used to determine concentration or chemical reaction order. Chromatography illustrates to students how the structure of compounds can help isolate them from others. When certain techniques are coupled together, the concepts are layered and even more can be learned about the system being studied.
Download Derivative Spectrophotometry and PAM-Fluorescence in by Vladimir S. Saakov, Alexander I. Krivchenko, Eugene V. PDF -...
4 consider the characteristics of derivative spectra of aromatic amino acids and proteins and also the features of spectral change under the influence of EFEs such as γ-radiation and temperature. In Chap. 5, the authors discuss both methodological questions of cell pigment spectroscopy and a number of debatable and evolutionary questions of pigment metabolism in representatives of various systematic groups of Eucaryota and Procaryota domains. In a separate section, the data of the last 20 years on features of carotenoid biosynthesis are presented. Biologists know well the world information base, the National Center for Biotechnology Information (NCBI) (www. gov), containing over 22 million significant and interesting scientific references. I. Sapozhnikov) are contained in this database and also two articles published 39 years later in 2004, where she was not the first, leading author (according to Russian rules of author placement) but only third or fourth. I. Sapozhnikov is a well-known figure ...
User:Sanuja Mohanaraj/Notebook/Experimental Biochemistry 1 Fall 2017/2017/10/17 - OpenWetWare
Figures (a) through (f) display the absorbance spectra of the gold nanoparticle mixed with each of the prepared reducing agents solutions. Figure (a) displays the absorbance spectrum of the AuNP-Cellulose nanocrystal solution, with no apparent peaks. Figure (b) displays the absorbance spectrum of the AuNP-Chitosan solution, with no observed peaks. Figure (c) displays the absorbance spectrum of the AuNP-Fructose solution, with an observed peak at wavelength 250nm. Figure (d) displays the absorbance spectrum of the AuNP-Galactose solution, with an observed peak at wavelength 250nm. Figure (e) displays the absorbance spectrum of the AuNP-H2AsCt solution, with a slight observed peak at wavelength 600nm. Figure (f) displays the absorbance spectrum of the AuNP-Na3Ct solution, with an observed steep peak at wavelength 550nm. ...
Diode-laser-based sensor for ultraviolet absorption measurements of atomic mercury | SpringerLink
A new sensor has been developed for measuring atomic mercury using absorption spectroscopy with 254-nm radiation generated from two sum-frequency-mixed diode lasers. Beams from a 375-nm external-cavit
Beckam Coulter DU 520 UV/Vis spectrophotometer at University of Puerto Rico - Medical Sciences Campus
with kinetic attachment Beckam Coulter DU 520 UV/Vis spectrophotometer is an eagle-i resource of type UV/vis spectrophotometer at University of Puerto Rico - Medical Sciences Campus.
Spectral study of two compounds of molybdate salt with beta-[Mo8O26] anion]. - Semantic Scholar
Two compounds of organic molybdate-alkali salt: [H(4,4-bipy)]2[K2Mo8O26](I) and H3 [Na Mo8 O26] (4,4-bipy)5 (H2O)4 (II), with beta-[Mo8O26]4- anion, were synthesized. The relationship between their properties and structures was studied by using FTIR, Raman, NIR-Vis and fluorescence etc. They possess isolating beta-[Mo8O26]4- anion, whose terminal O atoms combine with alkali. The FTIR and Raman spectra showed that the vibrational frequencies of the group are related to the structure of the materials. In UV-Vis spectra of compound (I) and (II), there is a characteristic wide peak at 280-300 nm. The fluorescence spectra of compound (I) and (II) were studied, which showed that the wide emission peak is from 450 to 650 nm and excited by 350 nm for compound (I), and the emission peak is from 400 to 550 nm and excited by 325 nm for compound (II). For compound (I), the intensity of emission becomes stronger with decreasing of temperature, and so does the fluorescence lifetime.
Modeling the 32 Electron Rule for Heavy Element Compounds - Software for Chemistry & Materials Software for Chemistry &...
Left: A [email protected] cluster. Right: UV-Visible spectra, with and without SO coupling.. After a first work on [email protected] Pb12 clusters, the authors modeled energies as well as UV/Vis and IR spectra of several actinide metals encapsulated in C28 cages through the use of DFT and TD-DFT. For modeling such compounds with these heavy elements, the relativistic effects implemented through ZORA are quite important, for instance in the spectroscopy of these compounds. Dr. Dognon has also indicated that the bond energy decomposition in ADF, the utility of the ADF GUI for building structures / analyzing results, as well as the good parallelization of the ADF program were all critical to the studies.. Do you want try for yourself? Request a free 30-day evaluation for the whole Amsterdam Modeling Suite.. ...
Mr. Tengfei Tian Profile
In this paper, we study the change mechanism of UV of three amino acids with positively charged R-group, which are processed under alternating electric field. We prepared 0.01mol/L aqueous solution of lysine(K), arginine(R), histidine(H) respectively, then disposed them at different frequency electric field for 5 minutes, scanning UV spectrum finally. As a result, red/blue shift and hyperchromic/hypochromic effect arise in spectrums of these three amino acids. Compared with control group, ΔλKmax is between -0.4nm and 0.8nm, ΔλRmax between -0.4nm and 0.8nm, ΔλHmax between -2.2nm and -0.6nm; ΔεKmax is between -2.657% and 2.143%, ΔεRmax between -4.997% and -1.581%, ΔεHmax between -2.098% and 0.314%. The levels of the amino acid molecules and the energy levels difference are affected by the excitation of alternating electric field. The changes of energy level difference result in the occurrence of UV spectrum red/blue shift. There is reduced trend of Δλmax along with increasing ...
Fluorescein | ISB Server Wahoo
MW = 332.31. Excitation wavelength: 460 nm Emission wavelength: 515 nm. Absorption maxima at 493.5 and 460 nm. A = Ecl. Molar extinction coefficient of fluorescein at 485nm = 50358/mol.cm. so Absorbance 10^-5M = 0.50358. (1/100 of a 1mM solution =10^-5M). 1 mM stock in 10mM NaOH. ...
How do I quantify my RNA sample? - xxpresspcr
where 40 is the average extinction coefficient for RNA. In addition, the A260/A280 ratio can be used to estimate RNA purity. An A260/A280 ratio between 1.8 and 2.1 indicates a highly pure RNA sample.. UV spectroscopy is relatively simple to perform but has several drawbacks. It does not discriminate between RNA and DNA so it is advisable to DNAse treat RNA samples before quantifying. DNA in the sample will lead to an overestimation of RNA concentration. Since proteins and residual phenol from the purification can interfere with absorbance readings, it is important to remove these contaminants in purification. Also, absorbance readings are dependent on pH and ionic strength. Dilute RNA samples in TE (pH 8.0) and use TE to blank the spectrophotometer before taking absorbance readings.. An alternative method for quantifying RNA samples is to use fluorescent dyes such as RiboGreen (Invitrogen). RiboGreen exhibits a strong fluorescent signal when bound to nucleic acids. Samples are quantified in a ...
Services - Medipharma Inc.
Rating active ingredients in pharmaceutical forms such as tablets , suspensions, syrups , capsules, etc. by methods such as high performance liquid chromatography (HPLC), UV spectrophotometry ( UV ), titration, and gravimetry ...
Patent US4481659 - Apparatus and method of reducing the bit rate of PCM speech - Google Patents
Bit rate in a PCM Companding system is reduced by transmitting sample magnitude (Xn) if the sample is near a zero-crossing, and transmitting the difference (Dn) between sequential samples if not near a zero-crossing. ROMs are used for mapping (transforming) full versus reduced data.
where nW, nY, and nC are the number of Tryptophans (W), Tyrosines (Y) and Cystines (i.e. disulphide bonds, but here denoted C) in the sequence. The molar extinction coefficients used in Innovagens Peptide Calculator are ...
LAMBDA 365 UV/Vis Spectrophotometer | PerkinElmer
The LAMBDA™ 365 delivers state-of-the-art UV/Vis performance that meets the needs of pharmaceuticals, analytic chemists, geneticists, and manufacturing QA/QC analysts everywhere. With 21 CFR part 11 compliant software available, the LAMBDA system is ready to support everything from standard methods and applications to those requiring regulatory compliance.
The UV/Vis+ photochemistry database | Spectroscopy Europe/Asia
The UV/Vis+ Photochemistry Database is a collection of photochemical data and information was started in 1999 and is now an online database updated weekly.
22 (67). Bibcode:1913POPot..67.....K. Loyd A. Jones (July 1927). "Photographic Spectrophotometry in the Ultra-Violet Region". ... ISBN 978-0-521-77096-5. W. M. H. Greaves (1936). "Time Effects in Spectrophotometry". Monthly Notices of the Royal Astronomical ...
Kondo, Y.; Henize, K. G.; Kotila, C. L. (1970). "Ultraviolet Spectrophotometry of Canopus from Gemini XI". The Astrophysical ... It was studied in the ultraviolet by an early astronomical satellite, Gemini XI in 1966. The UV spectra were considered to be ...
Orion (space telescope)
Gurzadyan, G.A. (1975). "Ultraviolet Spectrophotometry of the Emission Star SAO 0400183". Astronomy and Astrophysics. 39: 213. ... Ultraviolet spectrograms of thousands of stars to as faint as 13th stellar magnitude were obtained by a wide-angle meniscus ... Gurzadyan, G. A., Ultraviolet observations of planetary nebulae, Planetary nebulae. Observations and theory, Proc. IAU Symp.76 ... Ultraviolet telescope Optical system: Mersenne Spectrograph: Wadsworth Diameter of primary mirror: 280 mm Focal length: 1400 mm ...
September 2002), "Metallicity Determinations from Ultraviolet-Visual Spectrophotometry. I. The Test Sample", The Astrophysical ...
September 2002), "Metallicity Determinations from Ultraviolet-Visual Spectrophotometry. I. The Test Sample", The Astrophysical ...
Cary 14 Spectrophotometer
Sommer, L. (1989). Analytical absorption spectrophotometry in the visible and ultraviolet : the principles. Amsterdam: Elsevier ... To take readings in the ultraviolet or visible range, a hydrogen or tungsten lamp was used, focusing light into the entrance ... CS1 maint: discouraged parameter (link) Clarke, F. J. J. (June 5, 1972). "High Accuracy Spectrophotometry at the National ... CS1 maint: discouraged parameter (link) Hind, Andrew R. (December 2002). "To improvements in spectrophotometry. . " (PDF). ...
In particular, ultraviolet/visible (UV/Vis) spectrophotometry-based assays are widely applied. The most common UV/Vis ... spectrophotometry assay involves the monitoring of the formation of o-quinones, which are the products of polyphenol oxidase- ...
... based on the Astron ultraviolet spectrophotometry". Pis'ma v Astronomicheskii Zhurnal (in Russian). 12: 291-296. Bibcode: ... As a fraction of the gas molecules in the coma are ionized by the solar ultraviolet radiation, pressure from the solar wind, a ... Based on data retrieved by the largest ultraviolet space telescope of the time, Astron, during its Halley's Comet observations ... ISBN 978-0-521-64591-1. Woods, Thomas N.; Feldman, Paul D.; Dymond, Kenneth F.; Sahnow, David J. (1986). "Rocket ultraviolet ...
1986). "A model for the coma of Comet Halley, based on the Astron ultraviolet spectrophotometry". Pis'ma v Astronomicheskii ... based on the Astron ultraviolet spectrophotometry". Pis'ma v Astronomicheskii Zhurnal. 12: 696. Bibcode:1986PAZh...12..696B. A. ... The payload consisted of an 80 cm ultraviolet telescope, which was jointly bdesigned by the USSR and France, and an X-ray ... Based on the 4MV spacecraft design and operational for six years, Astron was the largest ultraviolet space telescope of its ...
Bohlin, R. C.; Gilliland, R. L. (2004). "Hubble Space Telescope Absolute Spectrophotometry of Vega from the Far-Ultraviolet to ... The UBV photometric system measures the magnitude of stars through ultraviolet, blue, and yellow filters, producing U, B, and V ... ISBN 978-0-226-10953-4. Michelson, E. (1981). "The near ultraviolet stellar spectra of alpha Lyrae and beta Orionis". Monthly ... ISBN 978-0-521-59889-7. Cochran, A. L. (1981). "Spectrophotometry with a self-scanned silicon photodiode array. II - Secondary ...
"Sur les spectres d'absorption ultra-violets des terres de la gadolinite". Comptes rendus de l'Académie des sciences. 87: 1062. ... "Uses for a Holmium Oxide Filter in Spectrophotometry" (PDF). Clinical Chemistry. 10 (12): 1117-20. PMID 14240747 ... Evaluation of Holmium Oxide Solution Reference Materials for Wavelength Calibration in Molecular Absorption Spectrophotometry ...
Hydrogen is easily detected in the ultraviolet (UV) and visible ranges from its absorption and emission of light (the hydrogen ... Advances in Standards and Methodology in Spectrophotometry. Burlington: Elsevier Science. p. 1. ISBN 978-0444599056. "A ... These mixed-molecular ices are exposed to ultraviolet radiation and cosmic rays, which results in complex radiation-driven ... However, these measurements have limitations, with various types of radiation (radio, infrared, visible, ultraviolet etc.) able ...
History of spectroscopy
Millikan, R.A.; Sawyer, R.A. (1919). "Three fourth of an Octave farther in the Ultra-violet". Science. 50 (1284): 138-139. ... Advances in Standards and Methodology in Spectrophotometry. Burlington: Elsevier Science. p. 1. ISBN 9780444599056. "The Era of ... Hoag, J.B. (1927). "Wavelengths of Carbon, Oxygen, and Nitrogen in the Extreme Ultraviolet with a Concave Grating at Grazing ... Millikan, R.A.; Sawyer, R.A. (1918). "Extreme ultraviolet spectra of hot sparks in high vacuum". Phys. Rev. 12 (2): 168. ...
"Fluorescence spectrophotometry of reduced phosphopyridine nucleotide in intact cells in the near-ultraviolet and visible region ...
Spectrophotometry Handbooks Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near- ... Traditional ultraviolet-visible spectroscopy or fluorescence spectroscopy uses samples that are liquid. Often the sample is a ... Fused quartz cells are used for wavelengths below 380 nm, i.e. ultraviolet light. IR quartz has a usable wavelength range of ... Today there are disposable plastic cuvettes made of specialized plastics that are transparent to ultraviolet light. Glass, ...
Ultraviolet fluorescence and infrared analysis are used to detect repairs or earlier painting present on canvasses. Atomic ... Absorption Spectrophotometry (AAS) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) are used to detect anomalies in ...
Nuclear Institute for Food and Agriculture
The NIFA administers cobalt-60 radiation source, Laser absorption spectrometer and Atomic Absorption Spectrophotometry, Near- ... infrared spectrometer and Ultraviolet-visible spectroscopy. A library was opened in 1990, and recently, the institute has ...
"In situ ultraviolet spectrophotometry for high resolution and long-term monitoring of nitrate, bromide and bisulfide in the ... "Direct ultraviolet spectrophotometric determination of total sulfide and iodide in natural waters", Analytical Chemistry, 73 ( ...
Barstow, M. A.; Holberg, J. B.; Koester, D. (1994), "Extreme Ultraviolet Spectrophotometry of HD16538 and HR:8210 Ik-Pegasi", ... Recent measurements with the Extreme Ultraviolet Explorer space telescope gave a more accurate orbital period of 21.72168 ± ... nm which lies in the far ultraviolet part of the electromagnetic spectrum. Brown, A. G. A.; et al. (Gaia collaboration) (August ... making it a strong source of ultraviolet radiation. Under normal conditions this white dwarf would continue to cool for more ...
... micrometers Ultraviolet and infrared energy sources Photometric colour determination equipment X-ray fluorescence and spectro-photometry ... Some inks may react to ultraviolet light, for example. Does the stamp have the correct tagging? Does the stamp have the correct ...
Arnold Orville Beckman
... use single instrument containing both the optical and electronic components needed for ultraviolet-absorption spectrophotometry ... on an instrument to find the energy of ultraviolet light. It worked by shining the ultraviolet light onto a thermocouple, ... There was also growing interest in examining ultraviolet spectra beyond that range. Just as Beckman had created a single easy- ... It produced accurate absorption spectra in both the ultraviolet and the visible regions of the spectrum with relative ease and ...
Museum Conservation Institute
... electron microscopy with X-ray and energy dispersive fluorescence X-ray radiography ultraviolet-visible light spectrophotometry ...
... from the ultraviolet spectral region to the visible and infrared Spectrometer Spectrophotometry Spectroscopy This ...
List of ASTM International standards (E)
16 Test Method for Ultraviolet Transmittance of Monoethylene Glycol (using Ultraviolet Spectrophotometry) E2193-RUS - 16 МЕТОД ... 20 Practice for Ultraviolet Conditioning of Photovoltaic Modules or Mini-Modules Using a Fluorescent Ultraviolet (UV) Lamp ... Method for Determination of Titanium in Iron Ores and Related Materials by Diantipyrylmethane Ultraviolet Spectrophotometry ... using Spectrophotometry) E2314 - 03(2014) Test Method for Determination of Effectiveness of Cleaning Processes for Reusable ...
List of MeSH codes (E05)
... spectrophotometry, ultraviolet MeSH E05.196.867.838 - spectroscopy, electron energy-loss MeSH E05.196.867.838.500 - microscopy ... spectrophotometry, ultraviolet MeSH E05.196.867.151 - circular dichroism MeSH E05.196.867.519 - magnetic resonance spectroscopy ... spectrophotometry, atomic MeSH E05.196.712.726.676 - spectrophotometry, infrared MeSH E05.196.712.726.676.700 - spectroscopy, ... spectrophotometry, atomic MeSH E05.196.867.826.676 - spectrophotometry, infrared MeSH E05.196.867.826.676.700 - spectroscopy, ...
Joseph Thomas Walker
"Quantitative Estimation of Barbiturates in Blood by Ultra-violet Spectrophotometry," The American Journal of Clinical Pathology ... "The Quantitative Estimation of Barbiturates in Blood by Ultraviolet Spectrometry," "Paper Chromatography in Criminal ...
... confirming the ring structure of hydantoins utilizing ultraviolet spectrophotometry techniques developed by her colleague Emma ... Her research utilized the then newly developed technique of ultraviolet spectroscopy to study hydantoins. Dorothy Hahn was born ...
List of ASTM International standards (D)
Test Method for Naphthalene Hydrocarbons in Aviation Turbine Fuels by Ultraviolet Spectrophotometry D1844 - 86(2020) Test ... Air Oxidation and Ultraviolet Fluorescence Detection D7621 - 16 Test Method for Determination of Hydrogen Sulfide in Fuel Oils ... Test Method for Ultraviolet Absorbance and Absorptivity of Petroleum Products D2010/D2010M - 98(2017) Test Methods for ... In-Line Ultraviolet Digestion and Amperometric Detection D7512 - 09(2015) Guide for Monitoring of Suspended-Sediment ...
... see also Spectrophotometry). For example, the Johnson UVB filters can be used to detect an ultraviolet (UV) excess in stars, ... Among stars of the same color, less metallic stars emit more ultraviolet radiation. The Sun, with 8 planets and 5 known dwarf ... Wallerstein, George; Carlson, Maurice (September 1960). "Letter to the Editor: on the Ultraviolet Excess in G Dwarfs". The ... Carney, B. W. (October 1979). "Subdwarf ultraviolet excesses and metal abundances". The Astrophysical Journal. 233: 211. ...
Surveys spectrophotometry Evidenziarono that the quartz alien sent well frequencies of ultraviolet deep, beautiful beyond the ... Infrared lenses Ultraviolet lenses Swivel lenses rotate while attached to a camera body to give unique perspectives and camera ... Some lenses have a UV coating to keep out the ultraviolet light that could taint color. Most modern optical cements for bonding ... "Ultraviolet Quartz Lenses". Universe Kogaku. Retrieved 2007-11-05. "Technical Room - Fluorite / UD / Super UD glass Lenses". ...
Analytical Absorption Spectrophotometry in the Visible and Ultraviolet, Volume 8 - 1st Edition
Purchase Analytical Absorption Spectrophotometry in the Visible and Ultraviolet, Volume 8 - 1st Edition. Print Book & E-Book. ... Analytical Absorption Spectrophotometry in the Visible and Ultraviolet, Volume 8 1st Edition. The Principles. 0.0 star rating ... Basis of Spectrophotometry in UV and VIS. 2. Principles of Instrumentation. 3. Errors in Spectrophotometry. 4. Evaluation of ... 7. Complexation and Spectrophotometry. 8. Some Applications of UV and VIS Spectrophotometry. Appendix. Subject Index. ...
Ultraviolet-Visible (UV-VIS) Spectrophotometry - Chemistry | Lycoming College
Ultraviolet-Visible (UV-VIS) Spectrophotometry. Ultraviolet-Visible (UV-VIS) Spectrophotometry. ... Ultraviolet-Visible Spectrophotometry (UV-VIS) is useful for both quantitative and qualitative work. The technique is based on ... Our particular instrument is a Cary Bio 50 ultraviolet-visible spetrophotometer, acquired in 2002 with funding from the ... the interaction of a sample compound with the ultraviolet and neighboring visible portions of the electromagnetic spectrum. The ...
Spectrophotometry, Ultraviolet | Profiles RNS
Ultraviolet" by people in this website by year, and whether "Spectrophotometry, Ultraviolet" was a major or minor topic of ... "Spectrophotometry, Ultraviolet" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... Below are the most recent publications written about "Spectrophotometry, Ultraviolet" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Spectrophotometry, Ultraviolet". ...
OSA | Noise analysis for CCD-based ultraviolet and visible spectrophotometry
Noise analysis for CCD-based ultraviolet and visible spectrophotometry John J. Davenport, Jane Hodgkinson, John R. Saffell, and ... "Noise analysis for CCD-based ultraviolet and visible spectrophotometry," Appl. Opt. 54, 8135-8144 (2015) ... In situ detection of water quality contamination events based on signal complexity analysis using online ultraviolet-visible ...
DETECTION OF ROSIN, ROSIN DERIVATIVES, AND PHENOLIC RESIN MODIFICATIONS IN ALKYD COATINGS USING ULTRAVIOLET SPECTROPHOTOMETRY.
Ultraviolet spectrophotometry is used to show the presence of rosin or ester gum modifications in the unsaponifiable portion of ... Abstract : Ultraviolet spectrophotometry is used to show the presence of rosin or ester gum modifications in the unsaponifiable ... AND PHENOLIC RESIN MODIFICATIONS IN ALKYD COATINGS USING ULTRAVIOLET SPECTROPHOTOMETRY. ... Descriptors : (*ULTRAVIOLET SPECTROSCOPY, *PLASTIC COATINGS), POLYESTER PLASTICS, PHENOLIC PLASTICS. Subject Categories : ...
Simultaneous Determination of Catechol, Resorcinol and Hydroquinone with Kalman Filtering Ultraviolet Spectrophotometry--...
... without preliminary separation.The ultraviolet absorption peaks of their aqueous solutions (pH=3.6) are 275nm,273nm and 288mp ... The ultraviolet spectrophotometry based on Kalman filtering(KF) was applied to determine simultaneously three isomeric ... The ultraviolet spectrophotometry based on Kalman filtering(KF) was applied to determine simultaneously three isomeric ... Simultaneous Determination of Catechol, Resorcinol and Hydroquinone with Kalman Filtering Ultraviolet Spectrophotometry. ...
Determination of Total Flavonoids in Zhitong Qufeng Jiaonang(止痛祛风胶囊)by Ultraviolet-Visible Spectrophotometry--《Rheumatism and...
... by ultraviolet-visible spectrophotometry.Methods:An ultrasound extraction with 70% ethanol was made for total flavonoids.The ... content of total flavonoids in Zhitong Qufeng Jiaonang was determined by ultraviolet-visible spectrophotometry with mangiferin ... The method of ultravioletvisible spectrophotometry for the determination of total flavonoids in Zhitong Qufeng Jiaonang is ... Determination of Total Flavonoids in Zhitong Qufeng Jiaonang(止痛祛风胶囊)by Ultraviolet-Visible Spectrophotometry. ...
Ultraviolet-Visible Spectrophotometry in Pharmaceutical Analysis | Taylor & Francis Group
OSA | Homochromatic Spectrophotometry in the Extreme Ultraviolet
Homochromatic Spectrophotometry in the Extreme Ultraviolet George R. Harrison and Philip A. Leighton ... George R. Harrison and Philip A. Leighton, "Homochromatic Spectrophotometry in the Extreme Ultraviolet," J. Opt. Soc. Am. 20, ... The Characteristics of Ultraviolet-Sensitized Photographic Plates in the Vacuum Ultraviolet* Po Lee and G. L. Weissler. J. Opt ... The d-log t curves of typical fast, medium, and slow emulsions when coated with oils fluorescing in the blue, near ultraviolet ...
Spectrophotometry - Wikipedia
UV-visible spectrophotometry. Main article: Ultraviolet-visible spectroscopy. Most spectrophotometers are used in the UV ... Although spectrophotometry is most commonly applied to ultraviolet, visible, and infrared radiation, modern spectrophotometers ... "Spectrophotometry". NIST. Retrieved Dec 23, 2018.. *^ a b c d e f g h i j Ninfa AJ, Ballou DP, Benore M (2010). Fundamental ... "Spectrophotometry Applications and Fundamentals". www.mt.com. Mettler-Toledo International Inc. Retrieved Jul 4, 2018.. ...
H30-UVL - HORIBA
Vacuum Ultra Violet Spectroscopy. There are several names given to wavelengths below the Ultra Violet. However, because of the ... Spectrophotometry. Clinical chemistry measurements are based on optical spectrometry for most of the blood molecules and also ... Vacuum Spectrometer: Monograph for far and extreme ultraviolet. Completing our offer in FUV monochromators, the H30-UVL allows ...
H20-UVL - HORIBA
Taylor & Francis India: Chemical Spectroscopy - Routledge
Ultraviolet Visible Near Infrared Spectrophotometry | Covalent Metrology Material Characterization Services
Online Analytical Absorption Spectrophotometry In The Visible And Ultraviolet The Principles
online analytical absorption spectrophotometry in the visible and ultraviolet on your theme or pay to the youTube item. turn ... Online Analytical Absorption Spectrophotometry In The Visible And Ultraviolet The Principles. A Private Lake Community, Rock ... Online Analytical Absorption Spectrophotometry In The Visible And Ultraviolet The Principles. by Christie 4.5 ... Youll take total to Enjoy the new online analytical absorption spectrophotometry in the visible and ultraviolet the once you ...
Lecture 5: Ultraviolet and Visible Spectrophotometry: Theoretical Aspects | CosmoLearning Chemical Engineering
Ultraviolet and Visible Spectrophotometry: Theoretical Aspects * Ultraviolet and Visible Spectrophotometry: Theoretical Aspects ... Ultraviolet and Visible Spectrophotometry: Applications * Fluorescence and Phosphorescence Spectrophotometry: Theoretical ... Fluorescence and Phosphorescence Spectrophotometry: Instrumentation * Fluorescence and Phosphorescence Spectrophotometry: ... Ultraviolet and Visible Spectrophotometry: Instrumentation * Ultraviolet and Visible Spectrophotometry: Instrumentation 2 * ...
Ultraviolet : Wikis (The Full Wiki)
Spectrophotometry. UV/VIS spectroscopy is widely used as a technique in chemistry, to analyze chemical structure, most notably ... Redirected to Ultraviolet (film) article). From Wikiquote. UltraViolet is a 2006 film about a young woman, Violet Song jat ... Ultraviolet lasers. UV laser diodes and UV solid-state lasers can be manufactured to emit light in the ultraviolet range. ... Ultraviolet radiation Ultraviolet light can be dangerous to ones health. For instance, sunlight contains UV rays that can ...
Near ultraviolet : Wikis (The Full Wiki)
Spectrophotometry. UV/VIS spectroscopy is widely used as a technique in chemistry, to analyze chemical structure, most notably ... Ultraviolet lasers. UV laser diodes and UV solid-state lasers can be manufactured to emit light in the ultraviolet range. ... Ultraviolet LEDs. Light-emitting diodes (LEDs) can be manufactured to emit light in the ultraviolet range, although practical ... False-color image of the Suns corona as seen in deep ultraviolet by the Extreme ultraviolet Imaging Telescope ...
Spectroscopic and Analytical Developments Ltd fingerprints brand spirits with ultraviolet spectrophotometry | Emerald Insight
NIOSHTIC-2 Publications Search - 20023776 - A simple device for measuring personal exposures to UV.
Development of methodology to aid in the exposure assessment of ultraviolet radiation in the workplace is the goal of the work ... Absorption-spectrophotometry; Light-source; Light-emission ... capable of determining the exposure of workers to ultraviolet ... Measurement-equipment; Workplace-monitoring; Exposure-assessment; Occupational-exposure; Testing-equipment; Ultraviolet- ...
Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry<...
Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry. Bulletin ... Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry. In: ... Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry. / Kim, ... title = "Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry", ...
Ultraviolet-visible spectroscopy - Wikipedia
Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy ... In addition, ultraviolet-visible spectrophotometry can be used to determine the thickness, along with the refractive index and ... The light source consists of a Xenon flash lamp for the ultraviolet (UV) as well as for the visible (VIS) and near-infrared ... If UV/Vis spectrophotometry is used in quantitative chemical analysis then the results are additionally affected by uncertainty ...
Spectrophotometry | physics | Britannica.com
Spectrophotometry, branch of spectroscopy that deals with measurement of the radiant energy transmitted or reflected by a body ... Ultraviolet spectrophotometry is particularly useful in detecting colourless substances in solution and measuring their ... Spectrophotometry, branch of spectroscopy that deals with measurement of the radiant energy transmitted or reflected by a body ... Spectrophotometry of protein solutions (the measurement of the degree of absorbance of light by a protein within a specified ...
Ultraviolet - wikidoc
Spectrophotometry. UV/VIS spectroscopy is widely used as a technique in chemistry, to analyze chemical structure, most notably ... Ultraviolet no:Ultrafiolett stråling om:Ultraviolet sh:Ultraljubičasto zračenje simple:Ultraviolet sk:Ultrafialové žiarenie sl: ... Ultraviolet light can be also generated by some light-emitting diodes. Natural sources of UV. The Sun emits ultraviolet ... False-color image of the solar corona as seen in deep ultraviolet light at 17.1 nm by the Extreme ultraviolet Imaging Telescope ...
SciCombinator - Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet...
Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry.. ... Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry. OPEN ... Ultraviolet-visible spectroscopy, Lower respiratory tract, Accuracy and precision, Upper respiratory tract, Respiratory system ...
EMC Promulgated Test Methods | Air Emission Measurement Center (EMC) | US EPA
Jacek Zielonka, PhD | Assistant Professor | Medical College of Wisconsin
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Reciprocity (photography) - Wikipedia
Quantitative Analysis of Steroids, Volume 5 - 1st Edition
7.2.1 Ultraviolet Spectrophotometry. 7.2.2 Colorimetric Methods. 7.2.3 Fluorimetry. 7.2.4 Column Chromatography. 7.2.5 Thin- ... 5.2.1 Ultraviolet Spectrophotometric Methods. 5.2.2 Colorimetric Methods. 5.2.3 Infrared Spectrophotometry. 5.2.4 NMR ... 2.2.1 Ultraviolet Spectrophotometric Methods Based on Natural Absorption. 2.2.2 Ultraviolet Spectrophotometric and Colorimetric ... 2.2.4 Infrared Spectrophotometry. 2.2.5 NMR Spectrometry. 2.2.6 Polarography. 2.2.7 Titrimetric Methods. 2.2.8 Gravimetric ...
SpectroscopyDosage forms by ultraviolet spectrophotometryAbsorptionInfrared radiationDerivativeWavelengthFluorescenceChemistryElectromagnetic spectrumSeen in deep ultravioletSpectroscopicConcentrationVisibleAbsorbanceQuantitationWavelengthsSpectrophotometersMolecularQuantitative analysisSolarQuantifyMethodMeasurementsVacuumLightAboardSurfaceAnalysisExperimentTabletsStudyExtreme
- Spectrophotometry is a branch of electromagnetic spectroscopy concerned with the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. (wikipedia.org)
- Spectrophotometry , branch of spectroscopy that deals with measurement of the radiant energy transmitted or reflected by a body as a function of the wavelength. (britannica.com)
- Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in part of the ultraviolet and the full, adjacent visible regions of the electromagnetic spectrum. (wikipedia.org)
- Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry ( UV/ VIS ) involves the spectroscopy of photons in the UV-visible region. (wikidoc.org)
- The instrument used in ultraviolet-visible spectroscopy is called a UV/vis spectrophotometer . (wikidoc.org)
Dosage forms by ultraviolet spectrophotometry1
- Prabu, L.S. / Simultaneous estimation for the analysis of salbutamol sulphate and ambroxol hydrochloride in solid dosage forms by ultraviolet spectrophotometry . (elsevier.com)
- Despite the existence of many competitive analytical techniques, molecular absorption spectrophotometry still remains very popular in practice, particularly in biochemical, clinical, organic, agricultural, food and environmental analyses. (elsevier.com)
- In astronomy , the term spectrophotometry refers to the measurement of the spectrum of a celestial object in which the flux scale of the spectrum is calibrated as a function of wavelength , usually by comparison with an observation of a spectrophotometric standard star, and corrected for the absorption of light by the Earth's atmosphere. (wikipedia.org)
- online analytical absorption spectrophotometry in the visible and ultraviolet on your theme or pay to the youTube item. (wanaksinklakeclub.org)
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- Although spectrophotometry is most commonly applied to ultraviolet, visible , and infrared radiation, modern spectrophotometers can interrogate wide swaths of the electromagnetic spectrum , including x-ray , ultraviolet , visible , infrared , and/or microwave wavelengths. (wikipedia.org)
- The terms chemical and heat rays were eventually dropped in favor of ultraviolet and infrared radiation , respectively. (thefullwiki.org)
- Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry. (scicombinator.com)
- In last three decades, derivative spectrophotometry has been extensively used in the determination of drugs in multi components having overlapping spectra, which eliminates interference from formulation matrix by using the zero-crossing techniques [ 1 - 4 ]. (biomedcentral.com)
- This study shows two simple, fast and alternative analytical methods for SILC determination by non-aqueous titration and by derivative ultraviolet spectrophotometry (DUS) in active pharmaceutical ingredient and/or dosage forms . (bvsalud.org)
- Ultraviolet ( UV ) light is electromagnetic radiation with a wavelength shorter than that of visible light , but longer than x-rays , in the range 10 nm to 400 nm, and energies from 3 eV to 124 eV. (thefullwiki.org)
- Ultraviolet ( UV ) light is electromagnetic radiation with a wavelength shorter than that of visible light , but longer than soft X-rays . (wikidoc.org)
- The plate is examined under ultraviolet light at a wavelength of maximum output at about 254 nm, then left in a chromatographic chamber containing some iodine crystals until spots appear. (who.int)
- UV radiation has been used to study the chemical structure of various substances and has been widely employed in visible spectrophotometry to determine the presence of fluorescence in a given sample. (medindia.net)
- Worsfold P.J. and Zagatto E.A.G., (2017) Spectrophotometry: Overview, , Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, Elsevier 2017, .doi: 10.1016/B978-0-12-409547-2.14265-9. (plymouth.ac.uk)
Seen in deep ultraviolet1
- False-color image of the solar corona as seen in deep ultraviolet light at 17.1 nm by the Extreme ultraviolet Imaging Telescope instrument aboard the SOHO spacecraft. (wikidoc.org)
- Connolly, C. (2006), "Spectroscopic and Analytical Developments Ltd fingerprints brand spirits with ultraviolet spectrophotometry", Sensor Review , Vol. 26 No. 2, pp. 94-97. (emerald.com)
- Ultraviolet (UV) - visible spectroscopic method of analysis is widely used in the analysis of drugs in pharmaceutical formulations and for dissolution and disintegration studies due to its good sensitivity and cost effectiveness. (biomedcentral.com)
- Ultraviolet spectrophotometry is particularly useful in detecting colourless substances in solution and measuring their concentration. (britannica.com)
- 1.1 This test method covers the determination, by ultraviolet spectrophotometry, of the total concentration of naphthalene, acenaphthene, and alkylated derivatives of these hydrocarbons in straight-run jet fuels containing not more than 5 % of such components and having end points below 315°C (600°F). This test method determines the maximum amount of naphthalenes that could be present. (globalspec.com)
- 1.1 This test method covers the determination, by ultraviolet spectrophotometry, of the total concentration of naphthalene, acenaphthene, and alkylated derivatives of these hydrocarbons in jet fuels. (globalspec.com)
- Concentration was tested using ultraviolet spectrophotometry. (arvojournals.org)
- Moreover, the method and its instrumentation has recently undergone considerable development resulting in some new special approaches of spectrophotometry in the ultraviolet (UV) and visible (VIS) regions. (elsevier.com)
- Ultraviolet-Visible Spectrophotometry (UV-VIS) is useful for both quantitative and qualitative work. (lycoming.edu)
- Our particular instrument is a Cary Bio 50 ultraviolet-visible spetrophotometer, acquired in 2002 with funding from the National Science Foundation. (lycoming.edu)
- John J. Davenport, Jane Hodgkinson, John R. Saffell, and Ralph P. Tatam, "Noise analysis for CCD-based ultraviolet and visible spectrophotometry," Appl. (osapublishing.org)
- Ultraviolet-Visible-Near Infrared spectrophotometry (UV-Vis-NIR) is a non-destructive, non-contact optical characterization technique used to measure reflectance, absorbance, and transmittance of liquids and solids. (covalentmetrology.com)
- Molecules containing bonding and non-bonding electrons (n-electrons) can absorb energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals. (wikipedia.org)
- We offer a complete line of Ultraviolet-Visible (UV-Vis) spectrophotometers. (thermofisher.com)
- It uses light in the visible and adjacent near ultraviolet (UV) and near infrared (NIR) ranges. (wikidoc.org)
- The basic parts of a spectrophotometer are a light source (often an incandescent bulb for the visible wavelengths, or a deuterium arc lamp in the ultraviolet), a holder for the sample, a diffraction grating or monochromator to separate the different wavelengths of light, and a detector. (wikidoc.org)
- The term as follows'' here indicates the contents of the Japanese Pharmacopoeia Fifteenth Edition from General Notices to Ultraviolet-visible Reference Spectra (p. 1 - 1654). (ebah.com.br)
- Choose from a complete line of ultraviolet-visible (UV-Vis) spectrophotometers. (thermofisher.com)
- High performance liquid chromatography and ultraviolet spectrophotometry for quantitation of tryptophan in barytic hydrolysates. (thefreedictionary.com)
- However, with the introduction of multicomponent methods, UV spectrophotometry showed an astonishing capability in the quantitation of compounds in mixtures without previous separation [ 8 - 11 , 15 - 17 , 24 ]. (ijpsonline.com)
- This would come as a solution to the previously created spectrophotometers which were unable to absorb the ultraviolet correctly. (wikipedia.org)
- Infrared spectrophotometry is most commonly used in studying the molecular structures of complex organic compounds . (britannica.com)
- Spectrophotometry is a tool that hinges on the quantitative analysis of molecules depending on how much light is absorbed by colored compounds. (wikipedia.org)
- It is produced continuously by the action of solar ULTRAVIOLET RAYS on oxygen in the stratosphere. (bioportfolio.com)
- After graduation he worked as a post doc at the University of California - Berkeley where he designed instrument for STUFF - the Solar/Terrestrial Ultraviolet?Flight Facility - a sounding rocket designed to measure the interaction of the Sun and the Earth's upper atmosphere. (uml.edu)
- In this study, we use spectrophotometry to quantify chemical differences in the water-soluble compound, coumarin. (usgs.gov)
- Our study aimed to quantify assay-based variation in G6PD spectrophotometry and to explore the diagnostic implications of applying a universal threshold. (malariaconsortium.org)
- Although there are a number of comprehensive textbooks dealing with UV/VIS spectrophotometry, they tend to describe historical aspects or contain collections of detailed procedures for the determination of analytes and do not reflect sufficiently the present state of the method and stage of development reached. (elsevier.com)
- One method based on direct ultraviolet spectrophotometry was put forward, which proved to be specific, linear, accurate and precise for the quality control of Naproxen suppositories, taking into account the presence of chromophore groups in their structure. (sld.cu)
- Spectrophotometry is often used in measurements of enzyme activities, determinations of protein concentrations, determinations of enzymatic kinetic constants, and measurements of ligand binding reactions. (wikipedia.org)
- The d - log t curves of typical fast, medium, and slow emulsions when coated with oils fluorescing in the blue, near ultraviolet, and far ultraviolet respectively, have been studied in the region 900 to 5000 Angstroms by means of a vacuum spectrograph designed especially for photographic photometry, together with ordinary quartz spectrographs. (osapublishing.org)
- The discovery of the ultraviolet radiation below 200 nm, named vacuum ultraviolet because it is strongly absorbed by air, was made in 1893 by the German physicist Victor Schumann . (thefullwiki.org)
- In order to determine the respective concentrations of reactants and products at this point, the light transmittance of the solution can be tested using spectrophotometry. (wikipedia.org)
- The research carried out here demonstrates that chemical actinometry using 3 mm ID quartz tubes along with a photometer constructed from a light emitting diode and a light sensitive diode, constitutes an inexpensive, accurate system capable of determining the exposure of workers to ultraviolet hazards. (cdc.gov)
- 10. The fiber optic sensing apparatus as recited in claim 8 wherein said light energy is in the ultraviolet light range. (google.com)
- In addition, he has been working to develop an Explorer mission to study ultraviolet light from the inter?and circum galactic medium. (uml.edu)
- The Hopkins Ultraviolet Telescope (HUT) was flown aboard the space shuttle Columbia on the Astro-1 mission from 1990 December 2--11. (stsci.edu)
- 98.7% of the ultraviolet radiation that reaches the Earth's surface is UVA. (thefullwiki.org)
- The significance of spectrophotometry in connection with liquid-liquid extraction, reaction kinetics, trace analysis, environmental and clinical analysis is also covered. (elsevier.com)
- An example of an experiment in which spectrophotometry is used is the determination of the equilibrium constant of a solution. (wikipedia.org)
- salicylic acid in tablets using ultraviolet spectrophotometry. (ijpsonline.com)
- For ASP stability monitoring in tablets, ultraviolet (UV) detector has been found to be fit for the purpose as it is suitable to accurately measure in the microgram order, therefore most of the methodologies reported previously employed high performance liquid chromatography (HPLC) technique [ 7 , 13 , 21 - 23 ]. (ijpsonline.com)
- IMAGER makes a four?color ultraviolet images in order study the creation and destruction of dust in the galaxy M101. (uml.edu)
- George R. Harrison and Philip A. Leighton, "Homochromatic Spectrophotometry in the Extreme Ultraviolet," J. Opt. (osapublishing.org)