Periodic Acid-Schiff Reaction: A histochemical technique for staining carbohydrates. It is based on PERIODIC ACID oxidation of a substance containing adjacent hydroxyl groups. The resulting aldehydes react with Schiff reagent to form a colored product.Alcian Blue: A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.Schiff Bases: Condensation products of aromatic amines and aldehydes forming azomethines substituted on the N atom, containing the general formula R-N:CHR. (From Grant & Hackh's Chemical Dictionary, 5th ed)Sialomucins: A subcategory of mucins that contain SIALIC ACID.Hematoxylin: A dye obtained from the heartwood of logwood (Haematoxylon campechianum Linn., Leguminosae) used as a stain in microscopy and in the manufacture of ink.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Periodic Acid: A strong oxidizing agent.Mucins: High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.Mucus: The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Solutions: The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Carmine: Coloring matter from the insect Coccus cacti L. It is used in foods, pharmaceuticals, toiletries, etc., as a dye, and also has use as a microscopic stain and biological marker.Pathology Department, Hospital: Hospital department which administers and provides pathology services.Thermodilution: Measurement of blood flow based on induction at one point of the circulation of a known change in the intravascular heat content of flowing blood and detection of the resultant change in temperature at a point downstream.No-Observed-Adverse-Effect Level: The highest dosage administered that does not produce toxic effects.Toxicity Tests, Subacute: Experiments designed to determine the potential toxic effects of a short-term (a few weeks) exposure to a chemical or chemicals.Toxicity Tests, Acute: Experiments designed to determine the potential toxic effects of one-time, short-term exposure to a chemical or chemicals.Toxicity Tests: An array of tests used to determine the toxicity of a substance to living systems. These include tests on clinical drugs, foods, and environmental pollutants.Purinergic P2Y Receptor Agonists: Compounds that bind to and stimulate PURINERGIC P2Y RECEPTORS. Included under this heading are agonists for specific P2Y receptor subtypes.Body Weight: The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.Anemia, Hemolytic, Autoimmune: Acquired hemolytic anemia due to the presence of AUTOANTIBODIES which agglutinate or lyse the patient's own RED BLOOD CELLS.Jatropha: A plant genus of the family EUPHORBIACEAE. Members contain jatrophone and other diterpenes.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Plant Oils: Oils derived from plants or plant products.Agricultural Irrigation: The routing of water to open or closed areas where it is used for agricultural purposes.Droughts: Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors.Gasoline: Volative flammable fuel (liquid hydrocarbons) derived from crude petroleum by processes such as distillation reforming, polymerization, etc.Oral Medicine: A branch of dentistry dealing with diseases of the oral and paraoral structures and the oral management of systemic diseases. (Hall, What is Oral Medicine, Anyway? Clinical Update: National Naval Dental Center, March 1991, p7-8)Posters as Topic: Single or multi-sheet notices made to attract attention to events, activities, causes, goods, or services. They are for display, usually in a public place and are chiefly pictorial.Embalming: Process of preserving a dead body to protect it from decay.Laser Therapy: The use of photothermal effects of LASERS to coagulate, incise, vaporize, resect, dissect, or resurface tissue.Mouth DiseasesLasers: An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.Tartrazine: An anionic, hydrophilic azo dye with an orange-yellow color used in fabrics, foods and cosmetics, and as a biological stain.Food Coloring Agents: Natural or synthetic dyes used as coloring agents in processed foods.Thyroid Gland: A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.Curcumin: A yellow-orange dye obtained from tumeric, the powdered root of CURCUMA longa. It is used in the preparation of curcuma paper and the detection of boron. Curcumin appears to possess a spectrum of pharmacological properties, due primarily to its inhibitory effects on metabolic enzymes.Azo CompoundsThyroid Neoplasms: Tumors or cancer of the THYROID GLAND.Thyroid Hormones: Natural hormones secreted by the THYROID GLAND, such as THYROXINE, and their synthetic analogs.Islets of Langerhans Transplantation: The transference of pancreatic islets within an individual, between individuals of the same species, or between individuals of different species.Rats, Inbred BB: A strain of Rattus norvegicus which is a model for spontaneous insulin-dependent diabetes mellitus (DIABETES MELLITUS, INSULIN-DEPENDENT).Adenoma, Liver Cell: A benign epithelial tumor of the LIVER.Carcinoma, Hepatocellular: A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.Islets of Langerhans: Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).Diabetes Mellitus, Type 1: A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Aldehydes: Organic compounds containing a carbonyl group in the form -CHO.Monosaccharides: Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent ... If the PAS stain will be performed on tissue, the recommended fixative is 10% neutral-buffered formalin or Bouin solution. For ... Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ... Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as ...
Schiff-type reagents are used for various biological tissue staining methods, e.g. Feulgen stain and periodic acid-Schiff stain ... The Schiff test is an early organic chemistry name reaction developed by Hugo Schiff, and is a relatively general chemical test ... When a sulfurous solution of rosaniline hydrate or rosaniline acetate is shaken with any aldehyde, then the yellow solution ... The further reaction of the Schiff reagent with aldehydes is complex with several research groups reporting multiple reaction ...
... and nail plate biopsy using periodic acid-Schiff stain. To reliably identify nondermatophyte molds, several samples may be ... In some cases, WSO is a misdiagnosis of "keratin granulations" which are not a fungus, but a reaction to nail polish that can ... Gupta AK, Paquet M (2014). "Efinaconazole 10% nail solution: a new topical treatment with broad antifungal activity for ... Dermatophytids can be thought of as an allergic reaction to the fungus. The causative pathogens of onychomycosis are all in the ...
Periodic acid-Schiff stain Detects glycoproteins as pink bands after electrophoretic separation. ... Measures the mechanisms underlying the biomolecular interactions, including reaction rates, affinities and associated ... and hetero-association to determine binding affinity and stoichiometry with proteins or carbohydrates in solution without ... Glycoproteins are proteins which contain oligosaccharide chains (glycans) covalently attached to amino acid side-chains. The ...
Jones' stain, a methenamine silver-Periodic acid-Schiff that stains for basement membrane, availing to view the "spiked" ... The reaction can be conducted in gas phase and in solution. The molecule has a symmetric tetrahedral cage-like structure, ... It is used in the Duff reaction (formylation of arenes), the Sommelet reaction (converting benzyl halides to aldehydes), and in ... As the mandelic acid salt (generic methenamine mandelate, USP) it is used for the treatment of urinary tract infection. It ...
Smears and biopsies are usually stained with periodic acid-Schiff, which stains carbohydrates in fungal cell walls in magenta. ... An alternative method of disinfection is to use a 10% solution of acetic acid (vinegar) as an overnight soak, or to microwave ... and is the site of cell mediated immune reactions. Competition and inhibition interactions between candida species and other ... Oral rinse involves rinsing the mouth with phosphate-buffered saline for 1 minute and then spitting the solution into a vessel ...
Periodic acid-Schiff staining is used to mark carbohydrates (glycogen, glycoprotein, proteoglycans). It is used to distinguish ... Lugol's solution or Lugol's iodine (IKI) is a brown solution that turns black in the presence of starches and can be used as a ... This method was discovered by Italian Camillo Golgi, by using a reaction between silver nitrate and potassium dichromate, thus ... Acid fuchsine[edit]. Acid fuchsine may be used to stain collagen, smooth muscle, or mitochondria. Acid fuchsine is used as the ...
... tartaric acid salt. The initial reaction, however, gives a poor yield and was improved by interaction of the two reagents at ... 59 °C. Coniine is slightly soluble (1 in 90) in cold water, less so in hot water, so that a clear cold solution becomes turbid ... Hemlock poisoning has been a periodic human concern, a regular veterinary concern, and has had significant occurrences in human ... a Schiff base differing from coniiine only by its carbon-nitrogen double bond in the ring. This pathway results in natural ...
Periodic acid-Schiff stain. Amyloid. *Congo red. Bacteria. *Gram stain *Methyl violet/Gentian violet ... Crystal violet (CV) dissociates in aqueous solutions into CV+. and chloride (Cl−. ) ions. These ions penetrate through the cell ... El-Garnal, A.H., Al-Otaibi, S.R., Alshamali, A., Abdulrazzaq, A., Najem, N., and Fouzan, A.A. Polymerase chain reaction is no ... "THE ACID FAST STAIN". www2.highlands.edu. Retrieved 2017-06-09.. *^ Beveridge, Terry J. (March 1990). "Mechanism of gram ...
Periodic acid Schiff (PAS) reaction of control group showed the thyroid glands from control group exhibited the magenta colored ... Group IV (recovery group): Included 10 rats that were received tartrazine solution at the same dose as group II once daily for ... Periodic acid Schiff (PAS) reaction for the demonstration of the optical density of colloid of thyroid follicle. ... the Periodic Acid and Schiff (PAS) staining procedure were used according to [22] to differentially stain the colloid inside ...
Includes: Alcian Blue pH 2.5 stain solution, periodic acid solution (0.5%) and Schiff reagent ... Periodic Acid Schiff (PAS) reaction is used to stain glycogen, basement membranes and neutral mucosubstances ... Can be used alone to stain acid mucins and other carboxylated and sulfated acid mucosubstances blue on tissue sections ...
... and alcian blue/periodic acid Schiff (PAS) reaction. Mucin was stained with mucicarmine solution; metanil yellow counterstain ... and alcian blue/periodic acid-Schiff (PAS) reaction (Troyer, H., "Carbohydrates" in Principles and Techniques of Histochemistry ... In addition, fatty acids (such as oleic acid or neutral fatty acids) can be used in the preparation of injectibles. Further, ... μg/kg of Compound HK-X in a solution of 0.1 M acetic acid at 1 mg/ml. On day 50, the mouse is exsanguated for histologic ...
In addition kidney sections were subjected by these rats of the Periodic Acid Schiff (PAS) reaction. The decalcification of the ... The following organs were fixed in Bouin solution, embedded in Paraplast and stained using hematoxylin-eosin (HE): aorta, eyes ... bones was performed in Ethylanadinitrilotetraacetic acid tetrasodium salt (EDTA).. In addition, tumor suspicious changes were ...
For histological observations, sections were stained with Periodic acid-Schiff (PAS) reaction for total insoluble carbohydrates ... Then capsules were surface sterilized in a 1% NaClO solution for 20 min and cut open to remove seeds for inoculation. ... Wang H, Xu JT (1993) A cytochemical study of acid phosphatase in the cortical cell of Gastrodia elata infected by Armillaria ... The seeds and developing mycorrhizal protocorms were fixed in a solution of 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH ...
PAS reaction (Periodic Acid-Schiff) of neutral polysaccharides. ... nm by a solution of 100 µL of sample/mL of Bradford solution. ... Protein extraction was performed by reaction with NaOH 0.1 M, and quantified by the Bradford method (1976). In this method an ... The quality of the biodiesel produced depends on the composition of fatty acids. These differ in three characteristics: 1) size ... The sugars were extracted using a sequence of reactions with 80% ethyl alcohol and quantified by readings from a ...
In addition kidney sections were subjected by these rats of the Periodic Acid Schiff (PAS) reaction. The decalcification of the ... The following organs were fixed in Bouin solution: Aorta, eyes, intestine (duodenum, jejunum, ileum, colon), femur, brain, ... A two year study on the acid form of the most similar substance has been considered as key and the related NOAEL selected for ... The same chronic study (Bomhard E. and Löser E., 1978) was also performed on the acid form of the disulphonated derivative (CAS ...
Sections were stained with hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) reaction and examined light microscopically by ... All specimens were fixed in 10% buffered formalin solution and embedded in paraffine. ...
Likewise, periodic acid-Schiffs (PAS) reaction and naphthol blue black test revealed enormous deposits of polysaccharides and ... This was followed by soaking in Schiff reaction solution for 20 min in the dark and rinsing four times in distilled water at pH ... Periodic acid-Schiff (PAS) reaction and naphthol blue black test were carried out to check for the presence of polysaccharides ... c and d are resin sections stained with periodic acid-Schiff (PAS) and naphthol blue black. a Cross section of a sepal. The ...
Massons trichrome or Gomoris trichrome or by the periodic acid Schiff (PAS) reaction with haematoxylin as counterstain. ... Pieces of ten placentae were fixed in Bouins solution or 10% formaldehyde and processed by standard histological procedures ... The protein they contain is thought to provide amino acids to the fetus. Given the similarity in ultrastructure, these ...
... acetic acid, incubating with 1% periodic acid in 3% acetic acid for 60 min, washing with water×4, reacting with Schiff stain ... the pH of the reaction solution is raised to a pH of about 7.4. The peptide-linker complex in solution at pH 7.4 can be added ... designer amino acids such as β-methyl amino acids, Cα-methyl amino acids, Nα-methyl amino acids, and amino acid analogs in ... The gel was then stained with the periodic acid-Schiff method by fixing overnight in 25% isopropanol-10% acetic acid, washing ...
Serial sections (2-3 μm) of the liver specimens were stained with H&E and with the periodic acid-Schiff (PAS) reaction. ... and the vessels were then rinsed for 2 minutes with Ringers solution, mixed with 0.5% procain and 4% dextran, followed by ... G, mixed cell focus; H, a very early HCA with nodular expansive growth and mitotic figures (arrowheads). A to H, PAS reaction. ... A, strongly positive PAS reaction in the liver acini downstream of the transplanted islet (arrow) owing to increased glycogen ...
The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent ... If the PAS stain will be performed on tissue, the recommended fixative is 10% neutral-buffered formalin or Bouin solution. For ... Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ... Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as ...
... and partly were treated for Periodic acid-Schiff reaction. The results were as followings: 1. The incomplete fusion of the ... and-Carnoys solution. Preparations were stained with alizarin red S, hematoxylin-eosin and alcian blue respectively, ... It might be concluded that the connective tissue under epithelium showed the decrease tendency to alcian blue and PAS reaction ... Periodic Acid-Schiff Reaction , Pregnancy , Rats ...
... material present in the UVD wall and lumen during the daily cycle of sperm release using the periodic acid-Shiff reaction (PAS ... Daily rhythms in accumulation and secretion of several glycoproteins are co-ordinated with periodic retention of sperm in the ... The biological significance of periodic sperm retention in the UVD lumen is not understood. In this study we asked whether ... sections were subjected to periodic acid-Schiff (PAS) reaction which detects carbohydrates in tissues, and Feulgen reaction to ...
... periodic acid-Schiff, hematoxylin-eosin, Feulgen reaction, silver-ion impregnation, Gomori s reticulin stain, toluidine blue, ... acetic acid (3:1) and kept at -20°C for 24 h. Roots were macerated in the enzyme solution of Flaxzyme (NOVO FERMENT )-distilled ... Amino Acid Sequence , Blotting, Western , Cell Nucleolus , Metabolism , Cell Nucleus , Metabolism , Cytoplasm , Metabolism , ... Fragment T4(Δ119-128) was generated from T4 with deletion of 10 amino acid of the C terminal. GFP fused with fragment T4(Δ119- ...
Periodic acid-Schiff reaction stains Mycobacterium tuberculosis, Mycobacterium leprae, Mycobacterium ulcerans, Mycobacterium ... 150 or 300 mM in the soil solution. The coping styles of five adult men within the context of a cancer-related social support ... Agrixal is acting by decreasing the amount of acid produced in the stomach. It is proton pump inhibitor (PPI). Agrixal is ... Enantioselective Diels-Alder reactions with G-quadruplex DNA-based catalysts. ...
... celloidin solution to preserve mucous gel and were stained by periodic acid-Schiff reaction for light microscopy. In weaned ... and the identical sections were stained by periodic acid-Schiff reaction. RESULTS: Plasma FITC-dextran level in NAC group was ... RESULTS: Morphologically, periodic acid-Schiff positive uniform mucus gel was present in front of Peyers patches of the ... Intestinal sections were stained by periodic acid-Schiff reagent. Peyers patches and MLNs were analyzed for the count of ...
An improved Jones PAS-M technique that utilizes an enhancer to accelerate the methenamine silver reaction with the glomerular ... Periodic Acid Schiff Methenamine Silver Stain for Basement Membranes. This comprehensive 8 component Jones PAS-M technique ... while reducing the time to achieve results with a more stabilized silver solution. Jones PAS-M is used in kidney biopsies, and ... utilizes an enhancer to accelerate the methenamine silver reaction with the glomerular basement membranes, ...
... periodic acid solution for 5 minutes.. 5. Wash in three changes of distilled water.. 6. Place in Schiff reagent for 15 minutes. ... 0.5% Periodic Acid. * Periodic acid ..... 2.5g. * Distilled water ..... 500.0 ml. * IN Hydrochloric Acid. o Hydrochloric acid, ... Add the acid to the water and mix well.. * Schiff Reagent. o Distilled water ..... 800.0 mL. o Basic fuchsin ..... 4.0 g. o ... The Schiff reaction has been described in the PAS procedure.. Fixative. 10% neutral buffered formalin, formalin alcohol, or ...
... or with the periodic acid-Schiff (PAS) reaction. No histological difference was observed in fruit treated with 2% CaCl2 ... In CaCl2 solutions up to 0.34 mol·L-1, turgor potential was ≥0.6 MPa in tissue incubated in 0.14 or 0.17 mol·L-1 solutions of ... L-1 solutions of CaCl2 and eliminated it in fruit treated with a 20 g·L-1 solution of CaCl2. While reducing the risk of calcium ... Fruit that were pressure infiltrated with CaCl2 solutions between 0.14 and 0.20 mol·L-1 and then waxed to reduce water loss ...
Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. ... A nuclear staining solution containing, hematoxylin, ferric chloride and hydrochloric acid used in many non-routine techniques ... OG-6 is a cytoplasmic counterstain solution used in sequence with EA (EA 50, EA 65 or EA36) in the Papanicolaou staining method ... Prussian Blue or Perls reaction is used to demonstrate ferric iron and ferritin. This is not a true staining technique rather ...
Renal sections were assessed for tissue injury using the periodic acid-Schiff (PAS) reaction as described (44). Evaluation was ... were prepared in antibody diluent solution (0.5% nonfat dry milk and 1% BSA in TBS) and incubated overnight at 4°C. The ... Effect of MnSOD knockdown on total tubular injury after I/R. A: representative 400× micrographs of periodic acid-Schiff (PAS) ... Kidney Morphology Based on Periodic Acid-Schiff Staining. ... All-trans retinoic acid alleviates hepatic ischemia/reperfusion ...
Periodic acid Schiff staining. Paraffin sections were deparaffinized, rehydrated, and then treated with periodic acid for 5 min ... Reverse-transcription polymerase chain reaction. Total RNA was extracted from two- to three-week-old cultures using TrizolTM ( ... The amniotic membrane was peeled off from the chorion, rinsed using Ringers solution, and placed on nitrocellulose paper, ... No goblet cells were seen in these cultures by periodic acid Schiff (PAS) staining (Figure 1D). ...
Specimens were then brought to the core pathology lab for embedding, cutting, and immunohistochemistry or periodic acid-Schiff ... The entire reaction was performed in 20 μl volume, which consisted of 10 μl SYBR green, 4 μl cDNA, 0.4 μl of each primer (10 μM ... Optimization of administration site and carrier solution was assessed. All animals received injections of Ambion in vivo siLRP2 ... The frozen kidney cortex was stored at −80°C for subsequent quantitative real-time polymerase chain reaction (qRT-PCR) and ...
  • More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. (emsdiasum.com)
  • Thus, reducing intracellular glutathione level, and increasing intracellular lipid peroxidation leading to oxidative damage to proteins and nucleic acids[ 6 ]. (ijpsonline.com)
  • If one shakes, with any liquid aldehyde, a red solution of the crystallized neutral sulfite [of fuchsine] or even the yellow solution which was prepared in the above-mentioned way and which contains leukaniline [i.e., fuchsine treated with aqueous sulfurous acid], then one obtains at once a red solution, and this color is transformed into a lighter or darker violet blue, depending on the aldehyde that's used. (wikipedia.org)
  • Place in Periodic Acid 0.5%, Aqueous ( Part 13308 ) for 10 minutes. (newcomersupply.com)
  • The silver staining technique for AgNOR nucleolar organizer regions (NORs) is characterized by marking proteins linked to the ribosomal ribonucleic acid, evaluating cell proliferation. (bvsalud.org)
  • Glycoproteins are proteins which contain oligosaccharide chains ( glycans ) covalently attached to amino acid side-chains. (wikipedia.org)
  • According to currently accepted ideas, vegetative storage organs such as bulbs, tubers, corms, rhizomes, and bark act as sinks for soluble nitrogen compounds (mainly amino acids) generated from the leaf proteins when the plant enters a senescing phase. (plantphysiol.org)
  • After transport through the phloem into the storage organs, the amino acids are incorporated into (storage) proteins in the storage parenchyma cells. (plantphysiol.org)
  • Environmental and/or endogenous stimuli induce a regulated degradation of the storage proteins, resulting in a massive release of amino acids that are subsequently transported to the new shoots to satisfy the high nitrogen demand of the rapidly growing tissues. (plantphysiol.org)
  • These amino acid analogs are fed to cultured cells and incorporated into proteins during active protein synthesis. (thermofisher.com)
  • It has been reported that L-carnitine inhibited free radical generation, prevented the damage due to fatty acid β-oxidation in mitochondria and protected tissues from impairment by renovating oxidized membrane lipids[ 10 ]. (ijpsonline.com)
  • Hepato-transcriptome analysis of mercury-exposed zebrafish revealed that the earliest deregulated genes were associated with electron transport chain, mitochondrial fatty acid beta-oxidation, nuclear receptor signaling and apoptotic pathway, followed by complement system and proteasome pathway, and thereafter DNA damage, hypoxia, Wnt signaling, fatty acid synthesis, gluconeogenesis, cell cycle and motility. (biomedcentral.com)
  • This work is to assess the role of L-arginin and omega-3 fatty acid against VPA oxidative stress on liver. (academicjournals.org)
  • Sodium valproate (VPA), an eight carbon branched chain fatty acid which commonly prescribed as antiepileptic drug for epilepsy, bipolar disorders and migraines (Tolou-Ghamari and Palizban (2015). (academicjournals.org)
  • Omega-3 fatty acid cannot be synthesized by humans and should be taken by diet named according to the location of double bond from the carbon of the terminal methyl called omega carbon (Plourde and Cunnane, 2007). (academicjournals.org)
  • The unsaturated fatty acids like eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) belong to the omega-3 fatty acid category. (academicjournals.org)
  • 2009). The omega-3 fatty acid has protective effects are by induction of cytochrome CYP7A1 expression and the activity of cholesterol catabolism to bile acids (Kim et al. (academicjournals.org)
  • 2012). In a study by Haast and Kiliaan (2015) a direct correlation was found between Omega-3 fatty acid intake and reduced liver deterioration and damage (Haast and Kiliaan, 2015). (academicjournals.org)
  • First, one reaction partner-either an azide or alkyne linked to a "building block" such as an amino acid, monosaccharide, fatty acid, nucleotide or nucleoside-is biosynthetically incorporated. (thermofisher.com)
  • 7. The method of claim 5 wherein the target molecule mimic is a peptide that binds to an 1D5 estrogen receptor-specific monoclonal antibody to produce a detectable reaction and comprising an amino acid sequence QXP, where X can be any amino acid. (google.com)
  • Analysis of the deduced amino acid sequence indicated that the C. sepium protein shows high sequence identity and structural similarity with plant RNases. (plantphysiol.org)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl-heidelberg.de)
  • Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. (emsdiasum.com)
  • Small bits of these tissues were placed in fixative, consisting of 1%osmic acid buffered with Ringer's solution and ELECTRON MICROSCOPY O F HUMAN P L A C E N T A 135 veronal to pH 7.5. (docme.ru)
  • L-carnitine assists as a cofactor necessary for the transport of long chain fatty acids into the mitochondria for energy production in the peripheral tissues[ 7 ]. (ijpsonline.com)
  • and derives its high degree of specificity from the fact that the azide and alkyne reaction partners have no endogenous representation in biological molecules, cells, tissues or model organisms. (thermofisher.com)
  • Conventional analysis relies on periodic acid-Schiff (PAS) staining of mucosal biopsy specimens, in which bacteria captured in foamy macrophages may be recognized. (asm.org)
  • Pieces of ten placentae were fixed in Bouin's solution or 10% formaldehyde and processed by standard histological procedures for embedding in paraplast, then sectioned at 7 μm (automatic microtome, Model RM2155, Leica, Germany). (biomedcentral.com)
  • In addition, Western blot, periodic acid-Schiff staining for evaluating goblet cell density, flow cytometry for counting the number of CD4+CXCR3+ T cells, and immunohistochemistry for detection of 4-hydroxy-2-nonenal (4HNE) were performed. (arvojournals.org)
  • The specimen should be placed on a microscope slide with a drop of potassium hydroxide 10% to 20% solution, then allowed to sit for at least five minutes before viewing under a microscope. (aafp.org)
  • Human pulmonary mucoepidermoid carcinoma (NCI-H292) cells and normal human bronchial epithelial cells were exposed to polyinosinic-cytidyric acid (poly(I:C)) and TGF-α. (jimmunol.org)
  • Bisulphite was believed to react with the available aromatic amine functional groups to form N-sulfinic acid groups, Ar-NH-SO2H, followed by reaction with aldehyde to form sulfonamides, Ar-NH-SO2CH(OH)-R. The 1980 NMR data that allowed visualization of intermediates does not support this mechanism or the sulfonamides as the chromogenic product. (wikipedia.org)
  • Fuchsin solutions appear colored due to the visible wavelength absorbance of its central quinoid structure-see also for example viologen -but are "decolorized" upon sulfonation of the dye at its central carbon atom by sulfurous acid or its conjugate base, bisulfite. (wikipedia.org)
  • Specific serum IgE testing can be used to assess sensitization and assist with diagnosis of food allergies if there is a history of an allergic reaction (i.e., anaphylaxis, acute urticaria, angioedema) to a particular food, but serum IgE testing alone is not adequate to diagnose food allergy. (oncologynurseadvisor.com)
  • A touch prep can provide immediate information, and is accomplished by collecting a punch biopsy and smearing the deep tissue edge on a slide, staining the slide with chlorazole black or 10% potassium hydroxide solution, and evaluating for the presence of hyphae. (renalandurologynews.com)
  • The decalcification of the bones was performed in Ethylanadinitrilotetraacetic acid tetrasodium salt (EDTA). (europa.eu)