An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.
A broad class of substances encompassing all those that do not include carbon and its derivatives as their principal elements. However, carbides, carbonates, cyanides, cyanates, and carbon disulfide are included in this class.
A plant family of the order Sapindales, subclass Rosidae, class Magnoliopsida. They are resinous trees and shrubs with alternate leaves composed of many leaflets.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Chromatographic techniques in which the mobile phase is a liquid.
A plant genus of the family ARALIACEAE. Ciwujia extract, which is prepared from plants of this genus, contains ciwujianosides and is used to enhance PHYSICAL ENDURANCE.
Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Spectrophotometric techniques by which the absorption or emmision spectra of radiation from atoms are produced and analyzed.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Chemical compounds which pollute the water of rivers, streams, lakes, the sea, reservoirs, or other bodies of water.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A solventless sample preparation method, invented in 1989, that uses a fused silica fiber which is coated with a stationary phase. It is used for sample cleanup before using other analytical methods.
Techniques used to synthesize chemicals using molecular substrates that are bound to a solid surface. Typically a series of reactions are conducted on the bound substrate that results in either the covalent attachment of specific moieties or the modification of existing function groups. These techniques offer an advantage to those involving solution reactions in that the substrate compound does not have to be isolated and purified between the reaction steps.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The surgical removal of a tooth. (Dorland, 28th ed)
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A space in which the pressure is far below atmospheric pressure so that the remaining gases do not affect processes being carried on in the space.
The branch of chemistry dealing with detection (qualitative) and determination (quantitative) of substances. (Grant & Hackh's Chemical Dictionary, 5th ed)
Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.
Examination of the mouth and teeth toward the identification and diagnosis of intraoral disease or manifestation of non-oral conditions.
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A form of SILICON DIOXIDE composed of skeletons of prehistoric aquatic plants which is used for its ABSORPTION quality, taking up 1.5-4 times its weight in water. The microscopic sharp edges are useful for insect control but can also be an inhalation hazard. It has been used in baked goods and animal feed. Kieselguhr is German for flint + earthy sediment.
A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
Tools used in dentistry that operate at high rotation speeds.
Organic compounds that have a relatively high VAPOR PRESSURE at room temperature.
A chemical process for separating the components of a liquid mixture by boiling and collecting condensed vapors.
The sumac plant family in the order Sapindales, subclass Rosidae, class Magnoliopsida. They are tropical and subtropical trees, shrubs, and woody vines that have resin ducts in the bark. The sap of many of the species is irritating to the skin.
Compounds with a core of 10 carbons generally formed via the mevalonate pathway from the combination of 3,3-dimethylallyl pyrophosphate and isopentenyl pyrophosphate. They are cyclized and oxidized in a variety of ways. Due to the low molecular weight many of them exist in the form of essential oils (OILS, VOLATILE).
A phase transition from liquid state to gas state, which is affected by Raoult's law. It can be accomplished by fractional distillation.
The study of the chemical and physical phenomena of radioactive substances.
Detection and counting of scintillations produced in a fluorescent material by ionizing radiation.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Devices or pieces of equipment placed in or around the mouth or attached to instruments to protect the external or internal tissues of the mouth and the teeth.

Trace analysis of ethinyl estradiol in casein diet using gas chromatography with electron capture detection. (1/374)

Ethinyl estradiol (EE2) is an extremely potent synthetic estrogen and a common component in oral contraceptives. The drug has a well-characterized pharmacological profile and is used as a positive control in toxicological investigations of compounds having estrogenic activity. An analytical method developed for the determination of low microg/kg levels of EE2 in a casein-based rodent diet is presented. A methanol extract of casein diet is purified for instrumental analysis by a 3-fold solid-phase extraction process. The sample extract is derivatized with pentafluoropropionic anhydride to the pentafluoropropionyl product and analyzed by capillary gas chromatography with electron-capture detection. Recoveries of EE2 from casein diet fortified at 5, 10, and 50 microg/kg average 88.8% and have a relative standard deviation (%) of 7.2. The method limit of detection in a casein-based diet is 1 microg/kg.  (+info)

Superheated water extraction of essential oils of Origanum micranthum. (2/374)

Superheated water extraction is used to extract essential oil of leaves of Origanum micranthum. The effect of different temperatures on the essential oil profile and rate of extraction as a function of time is investigated. The components of essential oil of Origanum micranthum are removed from the aqueous extract by C18 solid-phase extraction. The identification of components is carried out using comprehensive gas chromatography-time of flight-mass spectrometry. The number of extracted components is almost the same; however, the concentrations change with changing temperature. The highest yield (0.64%) is found at a temperature of 150 degrees C, 2 mL/min and 60 bar for 30 min. The increasing temperature from 100 degrees C to 175 degrees C increased the rate of extraction of six selected components of essential oil of Origanum micranthum. cis-Sabinenehydrate exhibits the fastest rate of extraction at all temperatures studied. Some degradation products are observed at a temperature of 175 degrees C.  (+info)

Development of an impregnated reagent and automation of solid-phase analytical derivatization for carbonyls: proof of principle. (3/374)

This study undertakes reduction of scale and automation of a solid-phase analytical derivatization of carbonyls with 2,4-dinitrophenylhyrazine on a styrene-divinylbenzene resin (XAD-2). Three processes are tested. In the batch process, an aqueous phase consisting of 50 microL of sample and 150 microL of reagent solution is contacted with 6 mg XAD-2 by shaking. An impregnated reagent consisting of 2,4-dinitrophenylhydrazine hydrochloride (DNPH) deposited on XAD-2 enables two additional processes. In-vial derivatization with an impregnated reagent requires shaking 50 microL of sample with 6 mg of the impregnated reagent and reduced the reaction time from 10 to 5 min. The third process involves packing impregnated reagent a mini-column and flowing 50 microL of sample through under positive pressure supplied by a Harvard Pump. This reduces sample preparation time to 1 min. Studies are conducted with worst-case model analytes: butanone, 2-pentanone, and malonyldialdehyde. The carbonyl of the two ketones is hindered, and, thus, these two compounds react very slowly with DNPH in aqueous solution. Malonyldialdehyde is highly water soluble, and it does not react in aqueous phase but also would not sorb onto XAD-2 for reaction. Nevertheless, derivatization/extraction of all model compounds any of the three procedures result in reproducible and high yields.  (+info)

Simplified method for determining the radiochemical purity of 99mTc-MAG3. (4/374)

99mTc-Mercaptoacetyltriglycine is used for dynamic renal imaging, and the summary of product characteristics (SPC) for the European formulation specifies a shelf life of 1 or 4 h, depending on the reconstitution volume of the kit. To minimize the time required to test the radiochemical purity, a simplified quality control method was developed. METHODS: To satisfy the recommendations of the International Commission on Harmonisation, results obtained with the methods described in the European and American SPCs were compared with those obtained with the simplified method. Further validation of the new method was performed by comparison with the standard 2-strip thin-layer chromatographic method as well as tests for linearity and limits of detection and quantification. RESULTS: The simplified method provided results comparable to those provided by the registered SPC methods but was more rapid to perform and used smaller volumes of solvents. CONCLUSION: The simplified method is a reasonable alternative to the registered SPC methods.  (+info)

Improved method of plasma 8-Isoprostane measurement and association analyses with habitual drinking and smoking. (5/374)

AIM: To develop a simple and accurate method for quantifying 8-isoprostane in plasma by employing a combination of two-step solid-phase extraction of samples and a commercially available ELISA kit, and by this method to examine the effects of drinking and smoking habits against the levels of plasma 8-isoprostane in healthy Japanese volunteers. METHODS: Plasma 8-isoprostane was extracted with ODS gel suspension followed by NH(2) Sep-Pak column. The 8-isoprostane fractions were assayed using a commercially available ELISA kit. We measured plasma 8-isoprostane levels in 157 healthy Japanese volunteers divided into three groups (64 non-habitual drinkers, 56 moderate drinkers and 37 habitual drinkers) according to their alcohol consumption per week. Genotypes of aldehyde dehydrogenase 2 (ALDH2) were also determined to investigate the plasma 8-isoprostane levels with reference to drinking habits. In addition, the plasma 8-isoprostane levels of 96 non-smokers and 61 smokers from the same subjects were compared. RESULTS: Our method fulfilled all the requirements for use in routine clinical assays with respect to sensitivity, intra- and inter-assay reproducibility, accuracy and dynamic assay range. Significant increases of plasma 8-isoprostane levels were observed in female habitual drinkers when compared with those of non-habitual drinkers (t = 5.494, P<0.0001) as well as moderate drinkers (t = 3.542, P<0.005), and 8-isoprostane levels were also significantly different between ALDH2*2/1 and ALDH2*1/1 in the female habitual drinkers (t = 6.930, P<0.0001), suggesting that excessive drinking of alcohol may increase oxidization stress, especially in females. On the contrary, no significant difference of the plasma 8-isoprostane levels was observed between non-smokers and smokers. CONCLUSION: Our present method was proved to be a simple and accurate tool for measuring plasma 8-isoprostane. However, the clinical utility of plasma 8-isoprostane for drinking and smoking habits was limited since elevated 8-isoprostane levels were observed in female heavy drinkers, and no association was found between smokers and nonsmokers.  (+info)

Evidence against a direct interaction between intracellular carbonic anhydrase II and pure C-terminal domains of SLC4 bicarbonate transporters. (6/374)

Based on solid-phase binding assays with enzyme-linked immunosorbent assay detection, previous investigators suggested that intracellular carbonic anhydrase II (CA II) interacts at high affinity with the C-terminal (Ct) domains of SLC4 bicarbonate-transport proteins, expressed as glutathione S-transferase (GST) fusion proteins, to form functional HCO3- metabolons. Here we re-evaluated this protein-protein interaction using two solid-phase binding assays. We first compared the ability of the Ct domain of three SLC4 transporters, SLC4-A1 (AE1), SLC4-A4 (NBCe1), and SLC4-A8 (NDCBE), to bind immobilized CA II, using enzyme-linked immunosorbent assay detection. We found that when expressed as GST fusion proteins, all three bind to CA II (Kd 300-600 nM) better than does pure GST. However, we detected no binding of pure SLC4-Ct peptides to immobilized CA II. Second, we reversed assay orientation by immobilizing the SLC4-Ct fusion proteins or peptides. We found that more CA II binds to GST than to any of the three GST-SLC4-Ct fusion proteins. Furthermore, we detected no binding of CA II to any of the immobilized pure SLC4-Ct peptides. Finally, we used surface plasmon resonance to detect possible rapid interactions between CA II and the pure peptides. Although we detected acetazolamide binding to immobilized CA II and specific antibodies binding to immobilized SLC4-Ct peptides, we detected no binding of CA II to immobilized SLC4-Ct or vice versa. Thus, although an HCO3 metabolon may exist, CA II cannot bind directly to pure SLC4-Ct peptides and can bind to GST-SLC4-Ct fusion proteins only when the CA II is immobilized and the fusion protein is soluble, and not vice versa.  (+info)

Control of matrix effects in the analysis of urinary F2-isoprostanes using novel multidimensional solid-phase extraction and LC-MS/MS. (7/374)

F(2)-isoprostanes (F(2)-iPs), established markers of oxidative stress, exist as four sets of regioisomers. Simultaneous and specific determination of F(2)-iPs can be achieved by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We developed novel methods for urine sample preparation and HPLC to control matrix-related ion suppression effects in the LC-MS/MS analysis of F(2)-iPs. A selective solid-phase extraction (SPE) wash protocol was developed with an Oasis HLB (hydrophilic-lipophilic balance) SPE cartridge using an elution profile of [(3)H]8-iso-prostaglandin (PG)F(2alpha) (iPF(2alpha)-III) when the methanol concentration was increased under acidic, neutral, and base wash conditions. A multidimensional (MD)-SPE method that incorporated size exclusion chromatography [corrected] reverse-phase chromatography, and normal-phase chromatography was developed using an Oasis HLB SPE cartridge and an HLB microElution SPE plate. Average extraction recoveries of the deuterated internal standards of iPF(2alpha)-III and iPF(2alpha)-VI were 62 +/- 8% and 60 +/- 10%. A buffer-free HPLC method for the separation of F(2)-iP isomers was developed on base-deactivated C8 columns. Average matrix effects for iPF(2alpha)-III and iPF(2alpha)-VI were 95 +/- 6% and 103 +/- 5%. The clean extraction of urine F(2)-iPs using MD-SPE and the separation of F(2)-iP isomers using a novel HPLC method did not cause apparent ion suppression in the analysis of iPF(2alpha)-III and iPF(2alpha)-VI using LC-MS/MS. These findings should be useful for establishing a routine LC-MS/MS method for the analysis of F(2)-iPs.  (+info)

Enantioanalysis of bisoprolol in human plasma with a macrocyclic antibiotic HPLC chiral column using fluorescence detection and solid phase extraction. (8/374)

A sensitive, enantioselective, high-performance liquid chromatographic (HPLC) method was developed and validated to determine S-(-)- and R-(+)-bisoprolol in human plasma. Baseline resolution was achieved using the teicoplanin macrocyclic antibiotic chiral stationary phase (CSP) known as Chirobiotic T with a polar ionic mobile phase (PIM) consisting of methanol-glacial acetic acid-triethylamine (100 : 0.02 : 0.025, v/v/v) at a flow rate of 1.5 ml/min and fluorescence detection set at 275 nm for excitation and 305 nm for emission. All analyses with S-(-)-atenolol as the internal standard were conducted at ambient temperature. The assay involved the use of a solid-phase extraction procedure for human plasma samples prior to HPLC analysis. The C18 cartridge gave good recovery rates for both enantiomers without any interference. The method was validated over the range of 20-200 ng/ml for each enantiomer concentration. Recovery rates for S-(-)- and R-(+)-bisoprolol enantiomers were in the range of 95-102%. The method proved to be precise (within-run precision expressed as % RSD ranged from 1.0-6.2% and between-run precision ranged from 0.9-6.7%) and accurate (within-run accuracies expressed as percentage error ranged from 0.2-4.8% and between-run accuracies ranged from 0.3-1.7%). The limit of quantitation and limit of detection for each enantiomer in human plasma were 20 and 5 ng/ml, respectively.  (+info)

Research Corridor has published a new research study titled Solid Phase Extraction Column Market - Growth, Share, Opportunities, Competitive Analysis and Forecast, 2017 - 2025. The Solid Phase Extraction Column market report studies current as well as future aspects of the Solid Phase Extraction Column Market based upon factors such as market dynamics, key ongoing trends and segmentation analysis. Apart from the above elements, the Solid Phase Extraction Column Market research report provides a 360-degree view of the Solid Phase Extraction Column industry with geographic segmentation, statistical forecast and the competitive landscape.. Browse the complete report at http://www.researchcorridor.com/solid-phase-extraction-column-market/. Geographically, the Solid Phase Extraction Column Market report comprises dedicated sections centering on the regional market revenue and trends. The Solid Phase Extraction Column market has been segmented on the basis of geographic regions into North America, ...
Get the latest solid phase extraction column news on Environmental XPRT, the worlds largest environmental industry marketplace and information resource.
The stationary phase comes in the form of a packed syringe-shaped cartridge, a 96 well plate, a 47- or 90-mm flat disk, or a microextraction by packed sorbent (MEPS) device, a SPE method that uses a packed sorbent material in a liquid handling syringe.[4][5] These can be mounted on its specific type of extraction manifold. The manifold allows multiple samples to be processed by holding several SPE media in place and allowing for an equal number of samples to pass through them simultaneously. In a standard cartridge SPE manifold up to 24 cartridges can be mounted in parallel, while a typical disk SPE manifold can accommodate 6 disks. Most SPE manifolds are equipped with a vacuum port, where vacuum can be applied to speed up the extraction process by pulling the liquid sample through the stationary phase. The analytes are collected in sample tubes inside or below the manifold after they pass through the stationary phase. Solid phase extraction cartridges and disks are available with a variety of ...
n automated method for analyzing free non-cholesterol sterols in human serum using online solid phase extraction-liquid chromatography-mass spectrometry is proposed herein. The method allows the determination of three phytosterols (sitosterol, stigmasterol and campesterol) and two cholesterol precursors (desmosterol and lanosterol). The analysis of sterols in human serum is critical in the study of cholesterol-related disorders, such as inherited familial hypercholesterolemias. Special effort was made to isolate the analytes from the serum lipoproteins, their natural conveyance through the bloodstream. The sample treatment consisted of a Bligh-Dyer extraction followed by dilution of the extract. This treatment allowed the sample to be injected into the online system and ensured the correct detection of the analytes, while avoiding the matrix effects commonly related to serum samples. The analytical performance showed linear ranges that covered two orders of magnitude, with correlation coefficients
Wastewater-treatment plants (WWTPs) are regarded as one of the main sources of antibiotics in the environment. In the present study, the concentrations of multiple antibiotics and their metabolites belonging to 5 antibiotic classes were determined in 3 major Finnish WWTPs. An online solid phase extraction-liquid chromatography-tandem mass spectrometry method was used for the extraction and analysis of the compounds. The method was fully validated using real and synthetic wastewaters. Seven antibiotics and 3 metabolites were found in the analyzed samples. Sulfonamides were removed most efficiently, whereas macrolides usually showed negative removal efficiency during the treatment, which means that the concentrations for individual antibiotics determined in the effluent samples were higher than in the influent samples. Sulfadiazine was found at concentrations up to 1018?ng/L, which was the highest concentration of any of the detected antibiotics in influent. In the effluent samples, the highest ...
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High levels of vanadium have been reported in groundwater (, 0.05 - 5300 g L-1 V) from different parts of Argentina, yet no detailed study of vanadium speciation has been performed. A highly selective strong anion exchange - solid phase extraction (SAX-SPE) method was used (in-situ) for vanadium speciation analysis of groundwater samples from La Pampa - LP (General San Martin and Eduardo Castex) and Buenos Aires - BA (San German) provinces in Argentina. In this method both vanadyl (VIV) and vanadate (VV) were trapped by the complexation with disodium ethylenediaminetetraacetic acid on a pre-conditioned SAX cartridge. In the laboratory, vanadium species were separated at different eluent pH levels. VIV was eluted at pH 4 using methanol and tetrabutylammonium hydroxide. VV was eluted at pH 8 using dihydrogen ammonium phosphate. The eluted species were analysed by inductively coupled plasma mass spectrometry (ICP-MS). This method was validated using an inter-analytical method comparison with ...
Solid phase extraction is a form of digital (step-wise) chromatography designed to extract, partition, and/or adsorb one or more components from a liquid phase (sample) onto stationary phase (sorbent or resin).
10.1002/jssc.201200466. Chapter 3 appears to be the equivalent of a post-print article finally published as: Candish, E., Wirth, H.J., Gooley, A. A., Shellie, R. A., Hilder, E.F., 2015, Characterization of large surface area polymer monoliths and their utility for rapid, selective solid phase extraction for improved sample clean up, Journal of Chromatography A, 1410, 9-18, 10.1016/j.chroma.2015.07.065 ...
Optimization Of Solid Phase Extraction By Experimental Design Methodology For Seperation of Sterol And Tocopherol In Sunflower Oil Deodorizer Distillate Using Zeolite As A Novel Sorbent (Sözlü Sunum ...
Data Bridge Market Research has added an exhaustive research study of the Global solid phase extraction market detailing every single market driver and intricately analyzing the business vertical. The research report has abilities to raise as the most significant market worldwide as it has remained playing a remarka...
Results for ACI solid phase extraction equipment for monitoring and testing. Compare and contact a supplier near you on (monitoring and testing) - Environmental XPRT
A rapid confirmatory method for determining 12 sulfonamide (SAs) antibacterials in whole milk and eggs is presented. This method is based on the matrix solid-phase dispersion technique with hot water as extractant followed by liquid chromatography (LC)-mass spectrometry (MS). The LC-MS instrument was equipped with an electrospray ion source and a single quadrupole. After 4 mL of a milk sample containing the analytes had been deposited on sand (crystobalite), this material was packed into an extraction cell. SAs were extracted by flowing 4 mL of water through the cell heated at 75 degreesC. With some modifications, this procedure was applied also to eggs. After pH adjustment and filtration, 0.5 mL of the final extracts was then injected into the LC column. MS data acquisition was performed in the positive-ion mode and by monitoring at least three ions for each target compound. The in-source collision-induced dissociation process produced confirmatory ions. At the 50 ng/g level, recovery of the ...
A high-throughput bioanalytical method based on automated sample transfer, automated solid phase extraction, and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) analysis, has been developed for the determination of cetirizine, a selective H1-receptor antagonist. Deuterated cetirizine (cetirizine-d8) was synthesized as described and was used as the internal standard. Samples were transferred into 96-well plates using an automated sample handling system. Automated solid phase extraction was carried out using a 96-channel programmable liquid-handling workstation. Solid phase extraction 96-well plate on polymer sorbent (Strata X) was used to extract the analyte. The extracted samples were injected onto a Betasil silica column (50 × 3, 5 μm) using a mobile phase of acetonitrile-water-acetic acid-trifluroacetic acid (93:7:1:0.025, v/v/v/v) at a flow rate of 0.5 ml/min. The chromatographic run time is 2.0 min per injection, with retention time of cetirizine and ...
Solid Phase Extraction (SPE) cartridges provide a fast and efficient sample clean-up and concentration prior to analysis through GC, HPLC, and/or other instrument methods. SPE method concentrates and purifies analytes from solution by absorption onto a disposable solid phase cartridge, followed by elution of the analyte with a solvent appropriate for instrument analysis.
Phenomenex Inc., a global leader in the manufacture of separation science consumables, introduces Strata Melamine, a solid phase extraction (SPE) sorbent specially designed for food testing. Strata Melamine SPE cartridges simultaneously extracts both melamine and cyanuric acid, cutting sample time in half compared to all other methods.
This thesis describes the development of liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for the trace analysis of active pharmaceutical ingredients (APIs) and their metabolites in aqueous environmental matrices. The research was focused on the development of chiral LC-MS/MS methods for the analysis of fluoxetine and metoprolol, as well as their chiral metabolites in environmental water samples. A method was also developed for the achiral compounds, diazepam and nordiazepam.. The LC-MS/MS methods were validated by the use of the isotope-labeled compounds. As these isotope-labeled compounds were not found in the wastewater samples, the validation could be assessed at trace level concentrations in the actual matrices in which the analytes were detected.. The analytes were extracted from the water samples using solid phase extraction methods. Different types of solid phase extraction sorbents were evaluated. Fluoxetine and norfluoxetine were extracted through the use of a mixed ...
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A simple, rapid and sensitive method for determination of trace levels of domoic acid (DA) in seawater was developed, based on a magnetic solid-phase extraction (MSPE) followed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Five kinds of ferrite magnetic nanospheres (MFe2O4; M=Fe, Co, Ni, Cu and Zn) were prepared and first used as sorbents for MSPE of DA and ...
Solid phase extraction has become a popular tool for analytical method development as a sample preparation technique. Solid phase sorbents can generally extract analytes based on polarity or selectively based on highly specific interactions between the analyte and an immobilized surface molecule. Biomolecular recognition based solid phases utilize antibodies, nucleic acids, and proteins as a secondary affinity layer on a solid phase support for selective analyte extractions and have been used for sample preparation, purification, separations, bioassays, and molecular diagnostics. Polymer supports for solid phase extraction are desirable for their low cost, abundance, chemical inertness, and pH stability. This thesis reports the use of capillary-channel polymer (C-CP) fibers as a solid support modified for selective extraction and on fiber fluorescence detection of nucleic acids and proteins. The polymer fibers are low cost and their unique shape allows them to be easily adapted into a fluidic fiber
D7168 - 16 Standard Test Method for 99Tc in Water by Solid Phase Extraction Disk , disc, disk, liquid scintillation counting, radioactivity, radiochemistry, solid phase extraction, technetium, water,,
Introduction. The common approach in risk assessment of water bodies is to collect a sample of the water, which is then analysed for potential pollutants in the laboratory. This approach, among other advantages, provides manageable control over accuracy and precision of the results. However, information obtained from spot water samples is only about concentration levels at the time of sampling and may fail to account for episodic contamination. This can be addressed by collecting a series of samples over a time period to obtain a more representative picture of water quality, but the cost of analysis is then increased. An alternative and more cost-effective approach is to obtain time-weighted average (TWA) concentrations of pollutants using passive samplers. Passive samplers are now seen as an alternative to conventional spot samples. This is seen by a number of recent reviews on passive samplers (Vrana et al., 2005; Lu et al., 2002; Petty et al., 2000; Stuer-Lauridsen, 2005; Chimuka and ...
Tetramethylenedisulfotetramine (TETS), commonly known as tetramine, is a highly neurotoxic rodenticide (the LD50, or lethal dose for 50 of test subjects, is 0.1 mg/kg). TETS has been used in hundreds of deliberate and accidental food poisoning events in China. In this report, we describe an extraction method for the quantitation of TETS as spiked into various food matrices, including fruit juices, egg, hot dog, chicken nuggets, turkey deli meat, and ground beef. Quantitation by liquid chromatographyion trap mass spectrometry was based upon selected reaction monitoring of mass-to-charge ratio (m/z) 347 268 and consecutive reaction monitoring of m/z 347268175. An 5 mg TETS concentration was spiked into each food sample. The linear range of quantitation for TETS was 0.520 g/mL. Total recoveries (and percent relative standard deviations) for TETS in various food samples are reported.
Discovery SPE products are available in 96-well plate configurations for high throughput parallel processing. Ideal for all application areas including Pharmaceutical, Clinical Analysis, Food and Beverage, Environmental, Petrochemical, Agriculture, and more.
Strata-X-PRO is a new innovative solid phase extraction (SPE) sorbent that offers a faster, cleaner way to extract your samples, completely revolutionizing traditional SPE methods. Strata-X PRO offers improved and rugged polymeric sorbent performance combined with matrix removal technology for a revolutionary solution. With a faster SPE method, it results in at least 40% reduction in time on your SPE protocol. Less steps with no conditioning or equilibration creates a fast SPE method without losing out on the importance of SPE: cleaning up your samples. Redefine Your Solid Phase Extraction with Strata-X PRO!. ...
analyzed the content with high resolution LC-MS. Chemometric methods revealed 87 compounds that constitute a specific chemical pattern either qualitatively or quantitatively indicating copepod presence. The majority of the compounds were present in both female and male exudates, but nine compounds were mainly or exclusively present in female exudates and hence potential pheromone candidates. Copepodamide G, known to induce defensive responses in phytoplankton, was among the ten compounds of highest relative abundance in both male and female extracts. The presence of copepodamide G shows that the method can be used to capture and analyze chemical signals from living source organisms. We conclude that solid phase ...
Sphingolipid extraction was performed as described [50] with modifications. Briefly, 500 larvae or 350 adult flies (both male and female were equally represented in control and treatment) were snap‐frozen in liquid nitrogen and homogenized by grinding. The homogenate was added to 201 g of methanol/chloroform/water mixture (3:1:1, v/v/v), followed by sonication for 20 min in room temperature. After overnight incubation, the extract was vortexed and centrifuged at 210 g for 10 min. The organic phase was dried by nitrogen stream, and the dried pellet was re‐dissolved in 2 ml of MeOH/water/CHCl3 (96:94:6, v/v) and applied to a Strata C18‐E solid‐phase extraction cartridge (SPE) (Phenomenex Inc., Torrance, CA, US) which was pre‐conditioned with 4 ml water. Two mL of MeOH was then added into the SPE cartridge after sample loading. A 3 ml of MeOH/CHCl3 (1:1, v/v) was added into the SPE cartridge to elute the sphingolipids. The MeOH/CHCl3 eluent was dried by nitrogen stream. The dried extract ...
Sphingolipid extraction was performed as described [50] with modifications. Briefly, 500 larvae or 350 adult flies (both male and female were equally represented in control and treatment) were snap‐frozen in liquid nitrogen and homogenized by grinding. The homogenate was added to 201 g of methanol/chloroform/water mixture (3:1:1, v/v/v), followed by sonication for 20 min in room temperature. After overnight incubation, the extract was vortexed and centrifuged at 210 g for 10 min. The organic phase was dried by nitrogen stream, and the dried pellet was re‐dissolved in 2 ml of MeOH/water/CHCl3 (96:94:6, v/v) and applied to a Strata C18‐E solid‐phase extraction cartridge (SPE) (Phenomenex Inc., Torrance, CA, US) which was pre‐conditioned with 4 ml water. Two mL of MeOH was then added into the SPE cartridge after sample loading. A 3 ml of MeOH/CHCl3 (1:1, v/v) was added into the SPE cartridge to elute the sphingolipids. The MeOH/CHCl3 eluent was dried by nitrogen stream. The dried extract ...
The present invention relates to fluorinated surfaces which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the surface. Generally, the hydrophilic and electropositive characteristics are expressed at the fluorinated surface. Preferred fluorinated surfaces of the present invention include fluorinated Al(OH)3, fluorinated SiO2 and fluorinated Celite. The fluorinated surfaces of the present invention are particularly useful in processes for purification of DNA from other cellular components. In these processes, a suspension of cellular components is placed in contact with the fluorinated surface, the fluorinated surface is washed to remove all cellular components other than DNA which are bound to the surface, and the bound DNA is eluted from the surface. Lower concentrations of chaotrope in the binding buffer are needed to bind DNA to the fluorinated surfaces.
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A variety of proven techniques, such as filtration, dialysis, liquid/liquid extraction and Solid Phase Extraction (SPE), are routinely adopted in todaylj analytical laboratories to resolve the vast array of sample preparation demands. SPE has established itself as one of the most popular and flexible tools within the analytical laboratory; providing effective, efficient sample concentration and purification prior to HPLC, LC/MS, GC or GC/MS and adaptation to automated techniques such as combinatory chemistry. The resultant SPE product range provides unparalleled performances and satisfies the clients demand for Recovery, Reliability and Reproducibility ...
Solid-phase extraction (SPE) is a technique designed for rapid sample preparation and purification prior to chromatographic analysis. You can optimize your SPE protocols by using SiliCycle SiliaPrepX Polymeric SPE Cartridges and Well Plates.
An efficient, rapid, and selective method for sample pretreatment, namely, molecularly imprinted matrix solid-phase dispersion (MI-MSPD) coupled with gas chromatography (GC), was developed for the rapid isolation of four phosphorothioate organophosphorus pesticides (tolclofos-methyl, phoxim, chlorpyrifos, and parathion-methyl) from carrot and yacon samples. New molecularly imprinted polymer nanomicrospheres were synthesized by using typical structural analogue tolclofos-methyl as a dummy template via surface grafting polymerization on nanosilica. Then, these four pesticides in carrot and yacon were extracted and adsorbed using the imprinted nanomicrospheres and further determined by gas chromatography. Under the optimized conditions, a good linearity of four pesticides was obtained in a range of 0.05–17.0 ng·g|sup|−1|/sup| with |i|R|/i| varying from 0.9971 to 0.9996, and the detection limit of the method was 0.012~0.026 ng·g|sup|−1|/sup| in carrot and
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In phase measuring deflectometry, two orthogonal sinusoidal fringe patterns are separately projected on the test surface and the distorted fringes reflected by the surface are recorded, each with a sequential phase shift. Then the two components of the local surface gradients are obtained by triangulation. It usually involves some complicated and time-consuming procedures (fringe projection in the orthogonal directions, accurate phase shifting).To avoid the complex process, a novel phase extraction algorithm with crossed fringes is presented in this paper. It is based on a least-squares iterative process. Both a numerical simulation and a preliminary experiment are conducted to verify the validity and performance of this algorithm. Experimental results obtained by our method are shown, and comparisons between our experimental results and those obtained by the traditional phase-shifting algorithm and between our experimental results and those measured by the Fizeau interferometer are made ...
An approach based on two-dimensional iterative nonlinear regression for retrieving phase information from single-frame interferograms was formulated and tested for fluid- and heat-flow measurements. Even though an initial crude phase assignment-i.e., fringe-order numbers at limited data points-is needed, the approach does not require complete phase unwrapping as in conventional techniques. Testing of computer-simulated and real interferometric data shows stable convergence and accurate phase extraction. The method works well under a high noise level, including broken fringes or contaminated regions, with a good noise-cleansing capacity. It provides accuracy at image- or opaque-object boundaries and directly offers spatial-gradient values. A weakness, however, can be intensive computation in the iterative estimation. The method is a good candidate for single-frame interferogram reduction.. © 1998 Optical Society of America. Full Article , PDF Article ...
CarboPrep Reversing SPE Cartridges cat.# 26206 High adsorbent capacity (surface area ~200 m2/g) for large volume sampling.Chromatographic grade graphitized carbon...
Having to reach ever lower limits of detection is a daily challange in modern laboratories. In order to succeed in obtaining sufficiently sensitive analysis methods, sample preparation techniques such as Solid Phase Extraction (SPE) or Liquid-Liquid Extraction are often used as concentration steps. The concentration factor achieved in these cases depends on the amount of solvent used for analyte elution from the SPE cartridge or for liquid extraction. Following the extraction step, further concentration of analytes can be achieved by reducing the amount of solvent left in the extract. This is typically achieved by evaporation. Such a concentration step can contribute significantly to improved limits of detection for the overall analytical method. For the evaporative concentration step, commercially available rotary evaporators as well as custom solutions are widely used. These are mainly stand-alone systems for manual operation. The GERSTEL MultiPosition Evaporation Station (mVAP) in combination ...
TY - CONF. T1 - A simplified SPE procedure combined with HPLC-ESI/MS for phospholipid determination. AU - Fauland, Alexander. AU - Trötzmüller, Martin. AU - Guo, Xinghua. AU - Köfeler, Harald. AU - Lankmayr, Ernst. PY - 2012/3/6. Y1 - 2012/3/6. M3 - (Old data) Lecture or Presentation. ER - ...
Introduction. Chloropropanol is a kind of toxic carcinogenesis generated in chemical production of soy sauce. It also has inhibitory effect on the production of male hormones which decrease the reproductive ability. It is greatly harmful to human body. Daily common three kinds are as following: 1-chloro-2-propanol (ClCH2CHOHCH3); 3-chloro-1,2-propanediol (3-MCPD) and 1,3- dichloro-2- propanol (1,3-DCP).. Experimental. Sample preparation. Accurately weigh 0.1 g chloropropanols standard substance in to100 mL volumetric flask, constant volume to scale by ethyl acetate and get 1 mg/mL stock solution. Gradually dilute the stock solution with acetone to get 1 μg/mL standard working solution. Weigh 290 g sodium chloride, add water to dissolve and dilute to 1000 mL, and then ultrasonic for 20 min. Weighs 2.5 g soy sauce directly loads to the Cleanert MCPD solid phase extraction column, static balance for 10min, wash the column with 15 mL ethyl acetate and collect the eluent. Nitrogen blow the eluent to ...
Metabolomics studies focus on the analysis of small molecules (MW,2000) in biological matrices from micro-organisms, plants, animals, and of human origin. Relatively large sets of samples are processed to allow differentiation between sample types and it is of course critically important to ensure that the analytical variability is lower than the biological variability. In order to achieve this, automating the sample preparation is a good first step, which can contribute significantly towards improving the repeatability of the total analytical procedure.. A typical metabolomics workflow includes extraction, fractionation or clean-up, derivatization, and a concentration step, followed by GC or LC separation and MS detection. In a series of articles, we describe a number of automated methods that are currently applied in our laboratories. In this first article, we focus on extraction and filtration. In a second article, an automatic fractionation procedure based on solid phase extraction will be ...
Service Level Agreement: Nutritional Supplements HFL Sport Science will provide the following services in accordance with the terms and conditions set out in the Master Services Agreement (or Informed-Sport / Informed-Choice License Agreements) agreed by both parties. Technical Description of Service Each sample is extracted and tested using GCMS or LCMS for the presence of substances detailed within Appendix 1, at the method capabilities / reporting levels specified. Technical Notes Sample Analysis • Each sample is tested for the presence of the drugs listed within appendix 1, at the Method • Sample preparation is by liquid-liquid and solid phase extraction techniques. • Internal marker(s) are added to each sample to assess suitability of matrix for testing • Analysis is conducted using gas chromatography with mass spectrometric detection (GCMS) and liquid chromatography with mass spectrometric detection (LCMS). • Testing is accredited to the ISO17025 standard for the following ...
Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight, FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly ...
TY - JOUR. T1 - Online solid phase extraction with liquid chromatography-tandem mass spectrometry for determination of estrogens and glucocorticoids in water. AU - Goh, Shalene Xue Lin. AU - Duarah, Ankur. AU - Zhang, Lifeng. AU - Snyder, Shane A.. AU - Lee, Hian Kee. N1 - Funding Information: This work was supported by the Singapore National Research Foundation under its Environmental & Water Technologies Strategic Research Programme administered by the Environmental & Water Industry Programme Office (EWI) of the Public Utilities Board, Singapore (Grant no. 706-000-016-490/706-000-016-133 ). S.X.L. Goh is grateful for the award of a graduate research scholarship from the EWI. Publisher Copyright: © 2016 Elsevier B.V.. PY - 2016/9/23. Y1 - 2016/9/23. N2 - The present work describes the development of a novel fully automated online solid phase extraction (SPE) coupled with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) using negative electrospray ionisation (ESI) for ...
Solid phase extraction (SPE) is one of the sample preparation methods most widely used by chromatographers, as demonstrated by the numerous published SPE methods found in the literature. Manual SPE cartridge formats can vary from disks through individual cartridges with a range of different volumes to 96-well plates. However, solid phase extraction methods can be tedious and time consuming when performed manually. There is therefore an increasing need for the automation of solid phase extraction methods. A robotic X-Y-Z coordinate autosampler commonly used for sample introduction in GC or HPLC can be used to perform a wide variety of sample preparation techniques using a single instrument and controlling software. The MAESTRO software allows the user to control the automation of solid phase extraction methods. In addition to ease of use and intuitive windows-based programming, the tools to optimize method parameters ensuring efficient sequence creation and maximum sample throughput. The sampler ...
A novel and highly sensitive method for the determination of some heavy metals in skin whitening cosmetics creams using multiwalled carbon nanotubes MWCNTs as solid phase extraction sorbent for the preconcentration of these heavy metals prior to their determination by inductively coupled plasma atomic emission spectrometry is described. Different practical parameters have been thoroughly investigated and the optimum experimental conditions were employed. The developed method was then applied for the determination of arsenic, bismuth, cadmium, mercury, lead and titanium in samples of skin whitening cosmetics. The detection limits under these conditions for As, Bi, Cd, Pb, Hg and Ti were 2.4, 4.08, 0.3, 2.1, 1.8, and 1.8 ng·mL−1, respectively. The relative standard deviations (RSDs) were found to be less than 2.0%. For validation, a certified reference material of NIST SRM 1570a spinach leaves was analyzed and the determined values were in good agreement with the certified values. The recoveries for
|p|This study is focused on the determination of some important antibiotics from different classes in waste water samples using solid phase extraction followed by high performance liquid chromatography with two detectors, diode array and mass spectrometer in positive ionisation mode. The investigated antibiotics include three penicillins (amoxicillin, ampicillin, penicillin G), two cephalosporins (ceftazidime, ceftriaxone), and two tetracyclines (tetracycline, doxycycline). The studied antibiotics were extracted from waste water samples using hydrophilic-lipophilic balanced cartridges. The extraction of antibiotics from water matrices was tested at several pH values. The best recoveries were obtained at pH 3 and 7 respectively. Depending on the nature of antibiotic, the limits of detection and quantification were obtained in the range of 0.07-0.92 µg mL−1 and 0.21-2.77 µg mL−1 respectively. Influent and effluent waste water samples were collected from a Waste Water
China Solid Phase Extraction (SPE) Consumables Market Research Report 2017 is a market research report available at US $3400 for a Single User PDF License from RnR Market Research Reports Library.
TY - JOUR. T1 - A rapid method for extraction of total lipids from whey protein concentrates and separation of lipid classes with solid phase extraction. AU - Vaghela, M. N.. AU - Kilara, A.. PY - 1995/10/1. Y1 - 1995/10/1. N2 - A modified procedure for extraction of total lipids from whey protein concentrates was developed such that stable emulsion with extracting solvents was avoided and the solvent system remained monophasic. Nonlipid contaminants from the extract were removed using gel filtration instead of traditional aqueous washing to prevent any loss of polar lipids. The extraction of total lipids by the modified procedure was complete and comparable with a reference procedure. Traditional thin-layer chromatography is tedious and more qualitative than quantitative for lipid class separation. Total lipids were further separated into free fatty acids, phospholipids, cholesterol ester, triacylglycerol, cholesterol, diacylglycerol, and monoacylglycerol, using modified solid phase extraction ...
Boom method (Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method is characterized by absorbing the nucleic acids (NA) to the silica beads. Boom method (Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid (NA) from biological samples. Essential of this method is the use of silica beads, capable of binding the NA in the presence of a chaotropic substance according to the effect. This method is one of the most widespread methods for isolating nucleic acids from biological samples and is known as a simple, rapid, and reliable method for the small-scale purification of NA from biological sample. This method is said to have been developed and invented by Willem R. Boom et al. around 1990. However, the aforementioned chaotropic effect itself was already known and already reported by Vogelstein and Gillespie before the development of the BOOM method. So the contribution ...
從台灣市場購得舒黨Su-Dangshen (Codonopsis tubulosa)和紋黨Wun-Dang.(C. pilosula),分別用水萃取後,再經solid phase extraction (SPE) 濃縮乾燥,利用核磁共振光譜儀(1H-NMR)分析時,從舒黨發現兩個新四級銨鹽codotubulosine A和codotubulosine B 生物鹼。本篇論文將台灣及中國大陸市場收集各類黨參樣品共42個,利用1H-NMR分析定量各樣品中codotubulosine A、codotubulosine B 生物鹼及adenosine 和5-(hydroxymethyl)furfural之含量。分析結果在黨參、素花黨參、川黨參中以codotubulosine A 為主要成分,在管花黨參中以codotubulosine A 和B 為主要成分,新疆黨參、羊乳、金錢豹和球花黨參均未發現 codotubulosine A 和 B;目前台灣及中國大陸市場常用藥材,將黨參、舒黨、素花黨參和川黨參視為主要商品。因此本論文將codotubulosine A 和 B生物鹼,建議作為黨參之指標成分。利用SPE結合1H-NMR可快速鑑定市售黨參藥材的品種
A fully automated and validated 2D online solid-phase extraction (SPE) high-performance liquid chromatographic method was described for the direct simultaneous determination of clenbuterol (CLEN) and doxazosin (DOX) in spiked human plasma samples. Chromatographic separation of the analytes was achieved by column-switching. SPE column packed with restricted access material for depleting matrix and a reversed phase HPLC column for eluting analytes were used with UV detection on 250 nm. A 10 mM acetate buffer (pH: 4) and acetonitrile mixture was used in gradient elution with 1 mL/min flow rate, as mass spectrometry friendly mobile phase. Calibration graphs were prepared for spiked plasma, and good linearity was achieved over a dynamic range of 75.0 ng/mL-200.0 mu g/mL for CLEN and 37.5 ng/mL-100.0 mu g/mL for DOX. Detection and quantification limits were 8.47 and 11.94 ng/mL, 25.66 and 36.18 ng/mL for CLEN and DOX, respectively. Online column-switching liquid chromatography technique combines the ...
An extraction cartridge, for extracting a component from a composition, contains a packing composition including a buffer composition, diatomaceous earth and absorbent. The cartridge is made by heating each component to assure it is free from contaminants. The diatomaceous earth is sifted to obtain a particular particle size. The cartridge also includes frits and filters for holding the packing composition within the cartridge. All of the components of the cartridge are selected or processed to assure that no contaminants are leached into the sample being extracted. A processing system automatically processes a plurality of cartridges. The processing system, which is capable of continuous operation, includes a platform, a rack for holding the cartridges, sample vials and extraction tubes. A walking beam conveyor system moves the rack along the platform and a system of robotic arms automatically transfers the sample to be extracted from the sample vial to the extraction cartridge and adds a predetermined
ISOLUTE® NH2 solid phase extraction cartridges work well for detecting alcohol biomarkers in human urine, and a recent study reported a successful throughput of 6,000 samples per year. The key advantage to this method is the elimination of the evaporation step prior to analysis by LC-MS/MS.. A new application note from Biotage (AN818.v1) offers some suggestions to optimize this method further, and also verifies the method on patient samples. The ISOLUTE NH2 cartridge format demonstrated as a viable option for urine measurements over a relevant concentration range in clinical reference laboratories.. ...
TY - JOUR. T1 - A case of death after ingestion of an agrochemical spreading agent. AU - Usui, Kiyotaka. AU - Hayashizaki, Yoshie. AU - Okubo, Yoshikazu. AU - Hashiyada, Masaki. AU - Funayama, Masato. PY - 2011/7/15. Y1 - 2011/7/15. N2 - An agrochemical spreading agent was found near the slightly decomposed corpse of a deceased female. The appearance of the stomach contents suggested that ingestion of a surfactant had occurred before death. The spreading agent was found to contain nonionic nonylphenol ethoxylates (NPEOn) and anionic sulfonated naphthalene-formaldehyde condensates (SNFCn). A solid phase extraction cartridge containing a mixed reversed phase-weak anion exchange sorbent (Oasis WAX, Waters) was used to successfully extract both NPEOn and SNFCn from the blood. The cartridge was preconditioned with methanol and acetic acid (AcOH). After the dilute blood sample was applied to the cartridge, it was washed with AcOH, and then NPEOn and SNFCn were eluted with methanol/dichloromethane ...
Eligible patients acquired their individualized dose of MTX on day 1 and blood samples had been collected for 48 h, until day 3, for your evaluation of MTX. Sufferers obtained thirty mg CP 690,550 every single twelve h from day 3 till day 6. On day 6, serial blood samples have been taken for examination STAT inhibitors of CP 690,550. On day 7, patients received their weekly MTX dose mixed with a 30 mg dose of CP 690,550, blood samples had been collected for your following 48 h for evaluation of CP 690,550 and MTX. Blood samples for PK examination of CP 690,550 were collected on day 1 at 0 h, days 6 and 7 at 0, 0. 25, 8 and twelve h, and also at 24 and 48 h publish day 7 dosing. Blood samples for PK examination of MTX have been collected on days 1?3 and days 7?9 at 0, 24 and 48 h.. Samples had been analysed for CP 690,550 concentrations making use of validated solid phase extraction followed by liquid chromatography/tandem mass spectrometry methodology. Samples had been analysed for MTX ...
Charlène Pouech, Florent Lafay, Laure Wiest, Robert Baudot, Didier Léonard, et al.. Monitoring the extraction of additives and additive degradation products from polymer packaging into solutions by multi-residue method including solid phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry analysis. Analytical and Bioanalytical Chemistry, Springer Verlag, 2014, 406 (5), pp.1493-1507. 〈10.1007/s00216-013-7551-4〉. 〈hal-00924245〉 ...
Abstract. INTRODUCTION: C-peptide is a widely used marker of endogenous insulin secretion. It is used for assessing residual beta cell function and in the diagnostic workup of hypoglycemia. C-peptide is routinely measured in the clinical laboratory by immunoassays, which are sensitive but prone to limitations such as cross-reactivity, between-lot variability, and a lack of concordance across different platforms. LC-MS/MS methods are more specific and can be multiplexed. Previous LC-MS/MS methods developed for serum c-peptide measurement detected intact peptide. Unfortunately, intact peptide is poorly ionized, requiring immunoaffinity extraction or solid phase extraction with two-dimensional chromatography. OBJECTIVE: The objective of this study was to develop and validate a novel enzyme (Glu-C) digestion-based LC-MS/MS assay for the quantification of serum c-peptide. METHODS: The internal standard was isotopically labeled at two leucine residues: EAED*LQVGQVELGGGPGAGSLQP*LALEGSLQ. Serum proteins ...
A sensitive method has been developed for the trace analysis of PAHs and their oxidation products (i.e., nitro-, oxy-, and hydroxy-PAHs) in air particulate matter (PM). Following PM extraction, PAHs, nitro-, oxy-, and hydroxy-PAHs were fractionated using solid phase extraction (SPE) based on their polarities. Gas chromatography-mass spectrometry (GC-MS) conditions were optimized, addressing inject ...
HPLC: high performance liquid chromatography; UV: ultraviolet detection; CE: capillary electrophoresis; FLD: fluorimetric detection; MS: mass spectrometry; MS/MS: tandem mass spectrometry; SPE: solid phase extraction; PLE: pressure liquid extraction ...
A simple solventless injection method was introduced based on the using of a solid-phase microextraction (SPME) fiber for injection of large amounts of the analytes extracted by the magnetic solid phase extraction (MSPE) procedure. The resulted extract from MSPE procedure was loaded on a G-coated SPME fiber, and then the fiber was injected into the gas chromatography (GC) injection port. This method combines the advantages of exhaustive extraction property of MSPE and the solvent-less injection of SPME to improve the sensitivity of the analysis. In addition, the analytes were re-concentrated prior to inject into the gas chromatography (GC) inlet because of the organic solvent removing from the remaining extract of MSPE technique. Injection of the large amounts of analytes was made possible by using the introduced procedure. Fourteen polycyclic aromatic hydrocarbons (PAHs) with different volatility were used as model compounds to investigate the method performance for volatile and semi-volatile ...
A fast and simple modified QuEChERS extraction method was developed for determination of Benzo[a]pyrene (BaP) in 137 traditional (Sangak), semi-industrial (Sangak) and industrial bread samples using spiked calibration curves by GC/MS. Sample preparation includes extraction of BaP into acetone followed by cleanup with dispersive solid phase extraction. The limit of detection and limit of quantification were 0.3 ng/g and 0.5 ng/g, respectively. The values for recoveries and RSD were calculated as 110.5-119.85% and ,11.6% respectively. Average concentration of BaP in Sangak bread samples of Shiraz and Tehran was 0.59 and 0.60 ng/g, respectively. 35.5% of samples of breads collected in Tehran were contaminated with BaP at the amount higher than maximum levels regulated in processed cereal-based foods and baby foods by European Commission (1 ng/g). Seventeen percent of samples of breads collected in Shiraz were contaminated with BaP which 13 % of total samples were ,1 ng/g. BaP content in all ...
Quantification of European Union (EU)-priority polycyclic aromatic hydrocarbons (PAHs) in plant matrices is a crucial task. Various methods for enrichment and preconcentration, such as the preloaded-pipette tip solid‑phase extraction (SPE) (1), are available. Nevertheless, analyte recovery as a result of homogenization, sample preparation, and extraction are rarely discussed in the field of phytopharmacy. This study deals with the recovery in dry plant extracts, which are typically used in phytopharmaceuticals and reflect the actual polycyclic aromatic hydrocarbon content in the commercially available end product (2). The aim of this study was to monitor benzo[a]pyrene, benzo[a]anthracene, chrysene, and benzo[b]fluoranthene loss of spiked samples as a result of commonly-used sample pretreatment, extraction, filtering, and evaporating techniques in 1:1 (v/v) cyclohexane-ethyl acetate primulae flos and sambuci flos dry extracts. Results showed that improper sample preparation can lead to false ...
Researchers at Griffith University in Australia have developed a new diffusive gradients in thin films (DGT) method to determine the concentration of antimony in polluted waters. This new technique also allows them to measure in situ the speciation of this metalloid in its different oxidation states. Speciation could be carried out before using solid phase extraction (SPE) cartridges, but these present some limitations when it comes to analysing some complex mixtures like seawater.. On the other hand, DGT is a sampling technique that can be used to measure a myriad of analytes in different mixtures. DGT uses a combination of hydrogel-based layers to extract and retain the analyte, that can be eluted and determined later on. This technique has been used to measure trace metals, metalloids, sulphides, phosphates and ammonium. In this case, to enhance the affinity of DGT membranes to antimony, researchers have used thiol-based silica gel layers.. In this paper, the authors present a combination of ...
Biotage QuEChERS products include extraction tubes for the initial extraction and partitioning of analytes from homogenized food samples, and clean up tubes for the dispersive solid phase extraction. All Biotage QuEChERS products come packed in a rack for ease of storage and use on the bench. Additionally, solvent resistant paper labels aid hassle free sample indentification. Biotage supplies pre-weighed extraction and clean up tubes that conform with AOAC1,2 and the EU3 published methods, including those for waxed and highly pigmented fruit and vegetable samples. ...
Between classes, Westerman would dash off to the lab in Thompson Hall to thaw waste-water samples in a water bath. In the afternoons, she prepared the samples in the trace analysis lab, where light streamed in from windows overlooking the glass-walled Oppenheimer Café and the Brown Family Courtyard. Decked out in lab coat, gloves, and safety glasses, and with Johnny Cash belting out mournfully through a speaker, Westerman would begin by putting a small volume of thawed wastewater samples through a filter to clean out the solids. It definitely didnt smell great, Westerman remembers. But it was a lot more dilute than the contents of toilet flush, she adds, and youd be surprised how quickly you get used to it. For the focused work of solid phase extraction, the next step of sample preparation, Westerman would fold her 6-foot-1-inch frame onto a lab stool in front of the fume hood. There, she put small amounts of the cloudy liquid through an array of tubes that looked like upside-down ...
This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using ISOLUTE® SAX strong anion exchange solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Very clean extracts are possible due to the purely anion exchange retention mechanism available using this silica based sorbent. This minimizes the co-extraction of endogenous interferences through hydrophobic (or non-polar) retention. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included ...
Problem] To provide a polymer which enables the solid phase extraction of a metal ion, particularly a rare earth element, and from which a metal of interest can be collected by burning the polymer after the adsorption of the metal of in ...
A broad range of organic compounds is recognized as environmentally relevant for their potential adverse effects on human and ecosystem health. This method was developed to better determine the distribution of 61 compounds that are typically associated with industrial and household waste as well as some that are toxic and known (or suspected) for endocrine-disrupting potential extracted from environmental sediment samples. Pressurized liquid extraction (PLE) coupled with solid-phase extraction (SPE) was used to reduce sample preparation time, reduce solvent consumption to one-fifth of that required using dichloromethane-based Soxhlet extraction, and to minimize background interferences for full scan GC/MS analysis. Recoveries from spiked Ottawa sand, commercially available topsoil, and environmental stream sediment, fortified at 4-720 ??g per compound, averaged 76 ?? 13%. Initial method detection limits for single-component compounds ranged from 12.5 to 520 ??g/kg, based on 25 g samples. Results from
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Polar Strong Anion (PSA) Exchange - AccuBONDII Sorbents for SPE Chromatography, Agilent Technologies : Bulk sorbents for packing SPE cartridges or to
α-Cobratoxin (CTX) is a large peptide (71 amino acids) with strong analgesic effect and may be misused in sports such as horse racing. To prevent such misuse, a sensitive method is required for detection and confirmation of the toxin in equine samples. CTX was extracted from equine plasma using an optimized mixed-mode solid-phase extraction (SPE) procedure. Extracted CTX was reduced with dithiothreitol and alkylated with iodoacetamide, and then was digested by trypsin at 56 C for 30min. The digest was analysed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and tryptic peptides T2 ( CFITPDITSK ) and T4 ( TWCDAFCSIR ) were monitored for detection and confirmation of CTX. The limit of detection (LOD) was 0.05ng/mL for CTX in plasma, and the limit of confirmation (LOC) 0.2ng/mL. Unlike small peptides consisting of the 20 canonical amino acids, CTX was stable in equine plasma at ambient temperature for at least 24h. The developed analytical method was successfully applied ...
Thermo Scientific™ HyperSep™ C18 Cartridges 5g Bed Weight; 25mL Column Volume Thermo Scientific™ HyperSep™ C18 Cartridges SPE Columns
Shop a large selection of SPE Columns products and learn more about Thermo Scientific HyperSep Cyano Cartridges 100mg Bed Weight; 1mL Column 100mg Bed Weight; 1mL Column Volume.
The skin sensitisation property of the registration substance, sodium N-lauroyl glutamate, is derived based on the read-across to sodium N-cocoyl glutamate and sodium N-cocoyl glycinate. The registration substance and the read-across sources are amides of fatty acids and amino acids and can be characterized as N-fatty acyl amino acids, of which endogeous occurence and metabolism are known. Based on the comparable chemical structure, comparable phys-chem data and expected comparable metabolism, these compound are likely exhibit comparable toxicity profiles. Sodium N-cocoyl glutamate was investigated for its skin sensitization property according to the OECD 406 (Buehler method). 0.5 ml of the test material wasoccusively applied to the clipped skin of guinea pigs for 6 hours as induction and challenge treatment. None of the twenty treated animals responded. Sodium N-cocoyl glutamate is not skin sensitizing. Sodium N-cocoyl glycinate was investigated for its skin sensitization property according ...
This thesis focuses on the use of molecularly imprinted polymers as selective sorbents for solid-phase extraction (MISPE). The MISPE methods developed were mainly intended for use with biological samples, such as human urine and blood plasma. These body fluids are complex samples, which often need an effective clean-up step before analysis to reduce the levels of possible interfering substances from the matrix, especially if the analytes are present in trace amounts. Solid-phase extraction (SPE) is a well-established and routinely used method for clean-up and preconcentration of samples from diverse matrices. However, conventional SPE sorbents often lack selectivity, leading to co-extraction of interferences, which negatively affects the following detection method. One of the advantages of MISPE is the built-in selectivity for a target analyte, or class of structurally related analytes, enabling the efficient clean-up that is often required for biological samples. The built-in selectivity of ...
CHROMFILTER is a Hongkong company composed by a well-trained working team having experiences in this profession for more than 20 years. As an excellent HPLC consumable products manufacturer, supplier and exporter, we have a factory in Songjiang District, Shanghai City. Our main products include all kinds of chromatography (GC/HPLC), laboratory filtration and laboratory accessories.CHROMFILTER designs, manufactures, distributes and sells a variety of laboratory products including syringe filters and membranes; sample vials and caps; SPE column; Disposable Vacuum Filtration system; sealing matts and other GC/HPLC high quality glass and plastic supplies.
CHROMFILTER is a Hongkong company composed by a well-trained working team having experiences in this profession for more than 20 years. As an excellent HPLC consumable products manufacturer, supplier and exporter, we have a factory in Songjiang District, Shanghai City. Our main products include all kinds of chromatography (GC/HPLC), laboratory filtration and laboratory accessories.CHROMFILTER designs, manufactures, distributes and sells a variety of laboratory products including syringe filters and membranes; sample vials and caps; SPE column; Disposable Vacuum Filtration system; sealing matts and other GC/HPLC high quality glass and plastic supplies.
This page lists B&O compatible phono cartridges, for other turntables we have a wide range of solutions see drop-down menus on page top for all our cartridges, audio products and services.. You have come to the best place for a solution. We are the only manufacturer of these cartridges, and we are authorized to do so under license by Bang and Olufsen. They are fully warranteed against manufacturing defect for one full year. Want to upgrade your cartridge and performance? Easily done. The B&O plug-in replacement cartridge design allows use of an interchangeable and wide range of cartridge models and performance for each turntable model - within each of the two groups below. (Group 1 = SMMC1, SMMC2, SMMC3, SMMC4 and The Voice; Group 2 = SMMC20E, SMMC20EN, SMMC20CL and SMMC20CL+) PLEASE NOTE; Use our model lookup tool to see which group your turntable needs. Cartridges purchased and opened (broken seal) cannot be returned for refund.. Do you want to use a B&O cartridge (Group 1) on a NON-B&O ...
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This article discusses the benef ts of using automated dispersive solid-phase extraction (dSPE) rather than standard SPE in urine analysis. ...
Solid-phase extraction for simultaneous separation and preconcentration of Fe(III) and Zn(II) traces using three chelatants and Ramelak bark-derived activated carbon as a new bio- ...
A fluid pumping assembly for particular use in delivery of sterile fluid used in surgical procedures, includes a pump control structure to which a replaceable fluid pumping cartridge is connected and disconnected to provide convenient replacement and disposability of the cartridge. The pump cartridge is self-contained in that it includes a fluid pump which is adapted for automatic connection to a pump drive structure when the cartridge is inserted into the assembly, so that the sterile fluid is pumped only through the pumping cartridge and fluid flow is entirely isolated from the pump control structure to ensure that the fluid is maintained sterile during pumping. The cartridge can include a selectively activated pulsation control to enable discharging the fluid from the cartridge in either a continuous flow or pulsating flow of the fluid. The cartridge can also include fluid flow dampening and safety vent features. The pump control structure can provide a pneumatic drive system which cycles pressurized
Supra-Poly SPE HLB Column, 60 µm, 200 mg/6 mL is a polymer-based spherical particle SPE media designed to extract mid-polar and non-polar compounds in aqueous and organic solvents.
"Graphitized carbons for solid-phase extraction". Journal of Chromatography A. 885 (1-2): 73-95. doi:10.1016/S0021-9673(00)00085 ... The most common use (70%) of carbon black is as a pigment and reinforcing phase in automobile tires. Carbon black also helps ...
Wells, M. J. M.; Yu, L. Z. (2000). "Solid-phase extraction of acidic herbicides". Journal of Chromatography A. 885 (1-2): 237- ...
This property can be useful in organic synthesis and separation methods such as solid phase extraction. In practice, a ... Zhang, W.; Curran, D. P. (2006). "Synthetic Applications of Fluorous Solid-Phase Extraction (F-SPE)". Tetrahedron. 62 (51): ... for fluorous phases or fluorous-derivatized solid phases allows for near complete recovery of the tagged reagent (i.e., near ... These reagents can then be separated from organic solvents by extraction with fluorinated solvents such as perfluorohexane. The ...
Mattivi F. (1993). "Solid phase extraction of trans-resveratrol from wines for HPLC analysis". Zeitschrift für Lebensmittel- ... Vrhovsek U. (1998). "Extraction of Hydroxycinnamoyltartaric Acids from Berries of Different Grape Varieties". J Agric Food Chem ... The amount of phenols leached is known as extraction. These compounds contribute to the astringency, color and mouthfeel of the ... The technique allows for a better extraction of phenolic compounds. The exposure of wine to oxygen in limited quantities ...
They have developed thin films for solid-phase micro extraction (SPME). Weber develops electrochemical detectors for use with ...
Sensitive GC-MS quantitation after solid phase extraction in 19 fatal cases". Forensic Sci. Int. 86 (3): 173-180. doi:10.1016/ ...
Silica resins avoid these issues through integration on microchips, where solid phase extraction provides accurate analysis of ... Toward a microchip-based solid-phase extraction method for isolation of nucleic acids. Electrophoresis, 23, 727-733 (2002).. ... Conventional methods for DNA extraction, such as ethanol precipitation or preparations using commercial purification kits, ... "Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized ...
... reverse osmosis and solid-phase extraction. Among them solid-phase extraction is considered as the cheapest and easiest ... Resuspension-induced partitioning of organic carbon between solid and solution phases from a river-ocean transition. Mar. Chem ... and a dissolved phase (DIC). PIC mainly consists of carbonates (e.g., CaCO3), DIC consists of carbonate (CO32-), bicarbonate ( ...
... is a solid phase extraction method for detection of pesticide residues in food. The name is a portmanteau word formed ... Following this, the sample is put through a dispersive solid phase extraction cleanup prior to analysis by gas-liquid ... Some modifications to the original QuEChERS method had to be introduced to ensure efficient extraction of pH-dependent ...
Solid phase extraction also can be used to isolate YTXs from the sample medium. This technique separates the components of a ... shellfish by high-performance liquid chromatography-tandem mass spectrometry based on enrichment by solid-phase extraction". ... This can be achieved by several methods: Liquid-liquid extraction or solvent extraction can be used to isolate YTXs from the ... The drawback of using the solvent extraction method is the levels of analyte recovery can be poor, so any results obtained from ...
Bioactive phenols in algae: The application of pressurized-liquid and solid-phase extraction techniques. L. Onofrejová, J. ...
Further fractionation of the extract can be achieved using solid phase extraction columns, and may lead to isolation of ... The application of pressurized-liquid and solid-phase extraction techniques". Journal of Pharmaceutical and Biomedical Analysis ... Phenol extraction is a processing technology used to prepare phenols as raw materials, compounds or additives for industrial ... Extraction can be performed using different solvents. There is a risk that polyphenol oxidase (PPO) degrades the phenolic ...
... is a membrane extraction process, where two solid phases are contacted across a membrane. The desired species in ... But the permeate is in liquid phase. Perstraction technique eliminates the problem of phase dispersion and separation ... Liquid-liquid extraction (LLE) was combined with the ABE fermentation for in situ product recovery, but the extractants with ... Perstraction, or membrane extraction, has been applied to many fields including fermentation, waste water treatment and alcohol ...
Albers S, Elshoff JP, Völker C, Richter A, Läer S (2005). "HPLC quantification of metoprolol with solid-phase extraction for ... thereby decreasing the slope of phase 4 in the nodal action potential (reducing Na+ uptake) and prolonging repolarization of ... phase 3 (slowing down K+ release). It also suppresses the norepinephrine-induced increase in the sarcoplasmic reticulum (SR) ...
"Determination of estazolam in plasma by high-performance liquid chromatography with solid-phase extraction" (PDF). Anal Sci. 18 ...
These separation methods include precipitation, Ion Exchange, Liquid Liquid extraction, Solid Phase extraction, Distillation, ... The advantage of solid state detectors is the greater resolution of the resultant energy spectrum. Usually NaI(Tl) detectors ...
Selective Solid-Phase Extraction of a-Tocopherol by Functionalized Ionic Liquid-modified Mesoporous SBA-15 Adsorbent. ... adsorption from supercritical gases in extraction processes and chromatography. Besides, knowledge of gas/solid interface ... The solid sample is placed in a sample holder on one arm of the microbalance while the counterpart is loaded on the other arm. ... However, adsorbed-phase density is the decisive factor for the existence of this end. The state of adsorbate at the "end" ...
An effective sample preparation protocol, usually involving either liquid-liquid extraction (LLE) or solid phase extraction ( ... Van Hout, MW; Hofland CM; Niederländer HA; de Jong GJ (2000). "On-line coupling of solid-phase extraction with mass ... "On-line coupling of solid-phase extraction with mass spectrometry for the analysis of biological samples. I. Determination of ... Similar is the effect of reducing the mobile phase flow rate to the nanolitre-per-minute range since, in addition to resulting ...
Trenbolone (trienolone) Waller CC, McLeod MD (2014). "A simple method for the small scale synthesis and solid-phase extraction ...
Curran also introduced fluorous solid phase extraction, a simple separation technique that enabled much subsequent work. The ... Curran introduced light fluorous reactions, fluorous triphasic reactions, fluorous phase vanishing reactions (with Ilhyong Ryu ... A fluorous-phase strategy for improving separation efficiency in organic synthesis" Science 1997, 275, 823-826. doi 10.1126/ ... and fluorous mixture synthesis (or FMS). FMS is the first example of solution phase synthesis with separation tagging, and it ...
Solid phase extraction which separates long polymers like DNA from other substances found in the cells. This is similar to ... The lag phase is not well known in microbiology, but it is speculated that this phase consists of the microorganism adjusting ... The log phase is the period where a culture experiences logarithmic growth until nutrients become scarce. The stationary phase ... where the solid phase is fixed and selectively binds a cellular component, allowing for its isolation. Gel electrophoresis is a ...
Izatt, R. M. Review of selective ion separations at BYU using liquid membrane and solid phase extraction procedures. J. Incl. ... Izatt, R. et al Solid phase extraction of ions of analytical interest using molecular recognition technology. Am. Lab. 1994 Vol ... Rahman, I. et al Determination of lead in solution by solid phase extraction, elution, and spectrophotometric detection using 4 ... Dulanská, S. Pre-concentration and determination of 90Sr in radioactive wastes using solid phase extraction techniques. J. ...
Estimates of bioavailability can also be obtained from chemical solid-phase soil extractions. Fugacity modelling of ... Ionic compounds, such as heavy metals, can be precipitated into the solid phase. Volatile compounds can be lost as aerosols to ... Depending on its chemical properties, a contaminant may or may not be found in the aqueous phase. Organic contaminants may ... bioavailability is based on the solubility and partitioning of compounds into aqueous and non-aqueous phases. This model ...
Barroso M, Gallardo E, Margalho C, Devesa N, Pimentel J, Vieira DN (April 2007). "Solid-phase extraction and gas ...
"Screening and determination of benzodiazepines in whole blood using solid-phase extraction and gas chromatography/mass ...
... is a solid phase extraction method to quickly purify nucleic acids. This method ... extraction. DNA separation by silica adsorption Guanidinium thiocyanate-phenol-chloroform extraction Ethanol precipitation ... relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions. The stages of the method ... A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder ...
"Determination of floral fragrances of Rosa hybrida using solid-phase trapping-solvent extraction and gas chromatography-mass ... This is because even numbered alkanes pack well in the solid phase, forming a well-organized structure, which requires more ... again depending on the ability of the alkane in question to pack well in the solid phase: This is particularly true for ... In plants, the solid long-chain alkanes are found in the plant cuticle and epicuticular wax of many species, but are only ...
"Determination of methylisoborneol in channel catfish pond water by solid phase extraction followed by gas chromatography-mass ...
"Trace-level determination of polar flavour compounds in butter by solid-phase extraction and gas chromatography-mass ... Sinha, A. K.; Sharma, U. K.; Sharma, N. (2008). "A comprehensive review on vanilla flavor: Extraction, isolation and ... who obtained it by evaporating a vanilla extract to dryness and recrystallizing the resulting solids from hot water. In 1874, ...
Solid-phase microextraction[edit]. Solid-phase microextraction (SPME), is a solid phase extraction technique that involves the ... Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. Solid phase extraction can ... Normal phase SPE procedure[edit]. A selection of solid phase extraction cartridges, available in many sizes, shapes, and types ... Solid phase extraction cartridges and disks are available with a variety of stationary phases, each of which can separate ...
Media in category "Solid phase extraction". The following 6 files are in this category, out of 6 total. ... Retrieved from "https://commons.wikimedia.org/w/index.php?title=Category:Solid_phase_extraction&oldid=139171874" ...
... a new generation of Solid Phase Extraction (SPE) 96-well microplates and cartridges that take SPE to a new level of performance ... by enhancing the reproducibility of analyte extraction and recovery from biological, environmental and chemical samples. ... Porvair Sciences announce Microlute™ CP - a new generation of Solid Phase Extraction (SPE) 96-well microplates and cartridges ... Highly reproducible solid phase extraction. News-Medical. https://www.news-medical.net/news/20200622/Highly-reproducible-solid ...
EMD Millipore™ LiChrolut™ Extraction Unit and Drying Attachment Accessories: PTFE Adapter. Luer inlet for solvent reservoir, ... EMD Millipore™ LiChrolut™ Extraction Unit and Drying Attachment Accessories: Large Volume Capillaries. for large-volume samples ... EMD Millipore™ LiChrolut™ Extraction Unit and Drying Attachment Accessories: Fluoroplastic Liners. For LiChrolut™ vacuum ... Solid Phase Extraction System Accessories. Thermo Scientific™ Dionex™ AutoTrace™ 280 Plunger Assemblies. Replacement plunger ...
... and/or adsorb one or more components from a liquid phase (sample) onto stationary phase (sorbent or resin). ... Solid phase extraction is a form of digital (step-wise) chromatography designed to extract, partition, ... Solid phase extraction is a form of digital (step-wise) chromatography designed to extract, partition, and/or adsorb one or ... Reversed-Phase SPE methodology. Ion-Exchange SPE methodology Normal Phase SPE methodology ...
Bond Elut solid phase extraction (SPE) products feature sorbents with phases for different analyte extraction and cleanup needs ... QuEChERS kits include extraction salts and dispersive SPE tubes ideal for food applications. ...
... readers will understand the power and usefulness of solid-phase extraction and how it helps solve sample preparation challenges ... read for anyone starting out in analytical chromatography as well as seasoned chemists looking to add solid-phase extraction to ... This 212 page book covers many topics including SPE device formats, sorbent considerations, mobile phase selection, and ...
Get the latest solid phase extraction column news on Environmental XPRT, the worlds largest environmental industry marketplace ... solid phase extraction column News. Related terms for "solid phase extraction column ": solid phase extraction news ... solid phase extraction (SPE), HPLC separation, and collection to deliver purified, concentrated fractions into standard NMR ...
Solid Phase Extraction Solid Phase Extraction Accessories & Vacuum Manifolds. Visiprep™ 5-port. Vacuum Manifold. SPE Tube ...
Currently, the solid phase of choice for solid phase extraction of DNA is Celite such as found in Prep-A-Gene™ by Bio-Rad ... DNA can also be purified using solid phases. Conventional solid phase extraction techniques have utilized surfaces which either ... Thus, it is desired to produce solid phase surfaces, particularly solid phases of fluorinated surfaces, which exhibit suitable ... DNA purification by solid phase extraction using fluorinated celite. US6177278. Dec 30, 1999. Jan 23, 2001. Norgen Biotek Corp ...
New trends in solid-phase extraction for analytical use--a practical introduction. Owing to its low cost, ease of use, and ... SPE in the 1970s: Extraction of Organic Pollutants from Water.. Solid Particles for Solid-Phase Extraction of Organic Compounds ... SOLID-PHASE EXTRACTION, Principles and Practice, E. M. Thurman and M. S. Mills 1998 (0-471-61422-X) 384 pp.. SOLID-PHASE ... New trends in solid-phase extraction for analytical use--a practical introduction.. Owing to its low cost, ease of use, and ...
Benefits of Solid Phase Extraction. Solid Phase Extraction (SPE) is widely used for the purification of small compound ... Solid Phase Extraction. This directory is limited to users with paid user access. Please refer to the page below for more ... The web site "Solid Phase Extraction in Organic Synthesis" is an excellent starting point for both a researcher new to the ... Home › Experimental Set-up and Purification › Work-up and Purification › Solid Phase Extraction ...
... a range of Solid Phase Extraction (SPE) 96-well microplates and cartridges designed to enhance t... ... For further information about the Microlute CP range of Solid Phase Extraction (SPE) 96-well microplates and cartridges please ... Porvair Sciences launches Microlute CP, a range of Solid Phase Extraction (SPE) 96-well microplates and cartridges designed to ... This is designed to enhance the flow-through of samples to maximise interactions between analytes and the solid phase to ...
Reproducible Solid Phase Extraction. 3 February 2012. This video features PerkinElmers Supra-Clean and Supra-Poly SPE ... Waters BioResolve RP mAb Polyphenyl Columns, Setting a New Standard for Reversed-Phase Separations... ...
Reproducible Solid Phase Extraction. 3 February 2012. This video features PerkinElmers Supra-Clean and Supra-Poly SPE ...
Abstract A silica monolith was developed as a solid-phase extraction sorbent for the extraction and separation of toxaphene ... congeners from soil extraction solutions, prior to their measurement by gas... ... A silica monolith was developed as a solid-phase extraction sorbent for the extraction and separation of toxaphene congeners ... Toxaphene congeners POPs Silica monolith cartridge Soil Solid-phase extraction This is a preview of subscription content, log ...
Results for ACI solid phase extraction equipment for monitoring and testing. Compare and contact a supplier near you on ( ... This is a special phase for the enrichment of carbonyl compounds. In this phase silica is impregnated with 2,4- ... ACI Solid Phase Extraction equipment for Monitoring and Testing * ACI - Americlean Reversible Cartridges. Americlean reversible ...
GARBARINO, JUAN A et al. VOLATILE COMPOUNDS OF LITHRAEA CAUSTICA (LITRE) DETERMINATED BY SOLID PHASE MICRO-EXTRACTION (SPME). ... The head space of the aerial parts of Lithraea caustica was analyzed by Solid Phase Micro-Extraction (SPME) technique, ... Palabras clave : Lithraea caustica; Anacardiaceae; Solid Phase Micro-Extraction; head space components; monoterpenes, ...
Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. Solid phase extraction can ... Solid-phase microextraction (SPME), is a solid phase extraction technique that involves the use of a fiber coated with an ... Solid phase extraction cartridges and disks can be purchased with several stationary phases, each of which separates analytes ... Guide to Solid Phase Extraction (PDF) Buszewski, Boguslaw; Szultka, Malgorzata (July 2012). "Past, Present, and Future of Solid ...
This article evaluates the use of commercially available cyanopropyl and octadecyl sorbents for the extraction of basic drugs ... Evaluation of solid-phase extraction of basic drugs from human milk J Pharm Biomed Anal. 1991;9(2):129-39. doi: 10.1016/0731- ... and subjected to a general solid-phase extraction (SPE) procedure described earlier for plasma samples. This SPE method was ... The extracts were further analysed by reversed-phase liquid chromatography (RP-LC). The application of SPE to human milk ...
The established routine analysis method employs Solid-phase Extraction with Absorption Spectrophotometry where the absorbance ...
The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the ... Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum Konstantinos M. Kasiotis 1 ... "Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum." Int. J. Mol. Sci. 9, no. ... The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the ...
The authors described a new application of amino-functionalized KIT-6 for dispersive ultrasonication-assisted micro solid phase ... extraction of hippuric acid (HA) and methyl hippuric acid (MHA) from... ... Post-derivatization procedure for determination of hippuric acid after extraction by an automated micro solid phase extraction ... Schematic of a sorbent for dispersive micro solid phase extraction coupled with ultrasonic power. It consists of amino ...
Method using solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) ... Method using solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS). ... and waste water containing less than 2 g/l solid particulate material (SPM) using liquid chromatography-tandem mass ...
Trace determination of fluoroquinolone antibacterial agents in urban wastewater by solid-phase extraction and liquid ... An analytical method based on reversed-phase liquid chromatography with fluorescence detection was developed and validated for ... Aqueous samples were extracted using mixed-phase cation-exchange disk cartridges that were subsequently eluted by ammonia ...
Tc in Water by Solid Phase Extraction Disk , disc, disk, liquid scintillation counting, radioactivity, radiochemistry, solid ... Standard Test Method for 99Tc in Water by Solid Phase Extraction Disk. Active Standard ASTM D7168 , Developed by Subcommittee: ... 1.1 This test method describes a solid phase extraction (SPE) procedure to separate 99Tc from environmental water (non-process- ... ASTM D7168-16, Standard Test Method for 99Tc in Water by Solid Phase Extraction Disk, ASTM International, West Conshohocken, PA ...
The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns ... Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery. ... The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns ... Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery. Journal of ...
In this study, a solid-phase extraction-type collection device, with styrene-divinylbenzene polymer particles (Sunpak-H) as the ...
Novel sol-gel hybrid methyltrimethoxysilane-tetraethoxysilane as solid phase extraction sorbent for organophosphorus pesticides ... of organophosphorus pesticides in bovine tissue by an on-line coupled matrix solid-phase dispersion-solid phase extraction-high ... TY - JOUR T1 - Novel sol-gel hybrid methyltrimethoxysilane-tetraethoxysilane as solid phase extraction sorbent for ... Sol-gel hybrid methyltrimethoxysilane-tetraethoxysilane as a new dispersive solid-phase extraction material for acrylamide ...
Finally we developed a convenient solid phase extraction method for the analysis of urine cellosolve metabolites. As a result, ... The current analytical method for urinary alkoxyacetic acid is liquid-liquid phase extraction. But the liquid-liquid phase ... We determined the appropriate extraction solvent and its flow rate. We also evaluated the non-absorptive rate and recovery rate ... Further evaluation of the recovery rate and non-absorptive rate according to the mini column shape, stationary phase and ...
  • Retrieved on November 27, 2020 from https://www.news-medical.net/news/20200622/Highly-reproducible-solid-phase-extraction.aspx. (news-medical.net)
  • Kasiotis KM, Souki H, Tsakirakis AN, Carageorgiou H, Theotokatos SA, Haroutounian SA, Machera K. Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum. (mdpi.com)
  • This document specifies a method for the determination of selected perfluoroalkyl and polyfluoroalkyl substances (PFAS) in non‑filtrated waters, for example drinking water, natural water (fresh water and sea water) and waste water containing less than 2 g/l solid particulate material (SPM) using liquid chromatography-tandem mass spectrometry (LC‑MS/MS). The compounds monitored by this method are typically the linear isomers. (iso.org)
  • Trace determination of fluoroquinolone antibacterial agents in urban wastewater by solid-phase extraction and liquid chromatography with fluorescen. (nih.gov)
  • An analytical method based on reversed-phase liquid chromatography with fluorescence detection was developed and validated for the simultaneous determination of nine FQs and the quinolone pipemidic acid in urban wastewater. (nih.gov)
  • In this study, a solid-phase extraction-type collection device, with styrene-divinylbenzene polymer particles (Sunpak-H) as the adsorbent, was used for the quantitative determination of phthalate esters in air samples. (go.jp)
  • Determination of urinary metabolites of alkyl cellosolves by solid phase extraction and GC/FID. (biomedsearch.com)
  • PEG 400 was either added to plasma prior to extraction at a concentration of 5 mg/mL or used to post-spike extracted blank plasma eluates for recovery determination. (waters.com)
  • Several main parameters affecting the efficiency of dt-MIP based dispersive solid-phase extraction (DSPE) were systematically investigated, coupled with high performance liquid chromatography determination. (rsc.org)
  • A novel and highly sensitive method for the determination of some heavy metals in skin whitening cosmetics creams using multiwalled carbon nanotubes MWCNTs as solid phase extraction sorbent for the preconcentration of these heavy metals prior to their determination by inductively coupled plasma atomic emission spectrometry is described. (mdpi.com)
  • In this article, a bioanalytical method for the extraction and determination of inositol hexaphosphate in whole blood is described by using solid-phase extraction followed by liquid chromatography-mass spectrometry/mass spectrometry using selected reaction monitoring mode for its specificity. (archives-ouvertes.fr)
  • A porous β -cyclodextrin polymer (P-CDP) was used as a new sorbent for simultaneous solid-phase extraction (SPE) and determination of bisphenol A (BPA), bisphenol F (BPF), and bisphenol AF (BPAF). (springer.com)
  • Determination of Morphine in Human Urine by A Simple Reverse Phase High-Performance Liquid Chromatography Method with UV Detection. (ebscohost.com)
  • A simple reverse-phase high-performance liquid chromatographic method has been developed for the determination of morphine in human urine using codeine as internal standard. (ebscohost.com)
  • A simple and inexpensive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with UV detection for determination of Cyantraniliprole and its metabolite (J9Z38) residues. (ebscohost.com)
  • Mixed-Mode Solid-Phase Extraction Procedures for the Determination of MDMA and Metabolites in Urine Using LC-MS, LC-UV, or GC-NPD. (ebscohost.com)
  • Extraction and Determination of Three Steroid Molecules in Milk Using Functionalized Magnetic. (deepdyve.com)
  • Determination of Thyreostats in Urine Using Supported Liquid Extraction and Mixed-Mode. (deepdyve.com)
  • Aims] An improved reversed phase high performance liquid chromatographic method for the determination of chlorotoluron in leave and root of wheat was established. (cnki.com.cn)
  • Online solid phase extraction and liquid chromatography-mass spectrometric determination of nucleoside drugs in plasma. (semanticscholar.org)
  • Soykan, C. Solidphase extraction of Pd(II) on a newly synthesized chelating resinprior to determination by flame atomic absorption spectrometry.Microchim. (edu.pl)
  • The present work describes the optimization and validation of a highly selective and sensitive analytical method using solid phase extraction and liquid chromatography tandem mass spectrometry (SPE LC-MS/MS) for the determination of some frequently prescribed pharmaceuticals in urban wastewater received and treated by Sharjah sewage treatment plant (STP). (preprints.org)
  • The method consists of a rapid protein precipitation by methanol followed by high throughput online solid phase extraction (SPE), ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS), and negative electrospray ionization detection. (uio.no)
  • A screening method automatically combining online solid-phase purification and concentration of samples with analysis using ultra-high performance liquid chromatography coupled with a hybrid quadrupole orbitrap mass spectrometer was developed and is reported in this paper. (separationsnow.com)
  • We developed an on-line solid phase extraction coupled to isotope dilution-high performance liquid chromatography-tandem mass spectrometry (on-line-SPE-HPLC-MS/MS) method for the quantification in urine of 1-OH-naphthalene, 2-OH-naphthalene, 2-OH-fluorene, 3-OH-fluorene, 1-OH-phenanthrene, the sum of 2-OH and 3-OH-phenanthrene, 4-OH-phenanthrene, and 1-OH-pyrene. (cdc.gov)
  • Solid-phase extraction ( SPE ) is an extractive technique by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties. (wikipedia.org)
  • The stationary phase of a reversed phase SPE cartridge is derivatized with hydrocarbon chains, which retain compounds of mid to low polarity due to the hydrophobic effect. (wikipedia.org)
  • However, a basic knowledge is necessary of how to perform SPE extractions in a efficient way by selecting the right sorbent (from a variety of reversed, normal or ion exchange phases), conditioning, selective washing and eluting the compounds of interest. (organicworldwide.net)
  • This is a special phase for the enrichment of carbonyl compounds. (environmental-expert.com)
  • The head space of the aerial parts of Lithraea caustica was analyzed by Solid Phase Micro-Extraction (SPME) technique, obtaining as main volatile compounds the monoterpenes, myrcene, a -pinene, , p- cymene and limonene, as well as the sesquiterpene caryophylene. (scielo.cl)
  • Therefore, SPE on CN or C18-sorbent for the analysis of basic compounds in breast milk was found to be useful as one of the steps in an extraction procedure, but not as a single technique. (nih.gov)
  • Five selected organophosphorus pesticides (OPPs) were employed as model compounds to evaluate the extraction performance of the synthesized sol-gel organic-inorganic hybrid MTMOS-TEOS. (unboundmedicine.com)
  • Biotage , a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the launch of the Atlantic ® ReadyDisk ® product family - solid phase extraction (SPE) disks, pre-assembled into single-use holders, for extracting semi-volatile organic compounds from aqueous samples. (labmate-online.com)
  • Storey, A. Evaluation of solid-phase micro-extraction coupled to gas chromatography-mass spectrometry for the headspace analysis of volatile compounds in cocoa products. (worldcocoafoundation.org)
  • Solid phase extraction is a technique used for the sample preparation through which compounds suspended or dissolved in the liquid sample are isolated from mixture of other compounds by their physical or chemical characteristics. (pharmiweb.com)
  • The passive sampler was found to be superior in its selectivity towards the target compounds compared to solid phase extraction technique with C 18 sorbent. (scielo.org.za)
  • Chromatograms from solid phase extraction of wastewater contained high peaks of unidentified, potentially interfering compounds, especially in the early part of the chromatogram. (scielo.org.za)
  • Volatile, polar, active, base-sensitive, and nonvolatile compounds are included to allow comprehensive evaluation of QuEChERS extraction and cleanup efficiencies, and optimization of GC and LC instrumental conditions. (restek.com)
  • In this work, superparamagnetic nanoparticle-decorated graphene (MG) sheets were synthesized and used as support for hemimicelles/admicelles for solid-phase extraction (SPE) of different compounds from environmental water samples for the first time. (diva-portal.org)
  • After solid-phase extraction (SPE), the compounds were separated on a reversed-phase Acentis Express C18 column (150mm. (ebscohost.com)
  • The four thyreostats mentioned above are polar and weak basic compounds (logP: −0.62-1.39, pKa: 4.41-8.24) and have small molecule weight (114-170 g mol−1), which give low retention on reversed-phase LC columns and less fragmentation in MS detectors (10). (deepdyve.com)
  • SiliCycle has developed the Silia PrepX ™ SCX, a strong cation exchanger polymer sorbent, for specific solid phase extraction of basic compounds ( pK a 2 - 10 ). (silicycle.com)
  • However, extraction efficiency can also be affected by uncontrollable factors such as batch to batch variation between fibers of the same type and presence of compounds in the sample matrix, which competes with the compounds of interest for adsorption to the SPME fiber. (dtu.dk)
  • The newly synthesized polymeric materials can be employed as SPE sorbents for efficient extraction of polyphenolic compounds especially for flavonoid aglycons. (edu.pl)
  • Obtain improved extraction of aromatic compounds and basic analytes with these phenyl SPE cartridges offering alternative selectivity for retention of basic compounds. (fishersci.at)
  • Unique graphite carbon-based SPE cartridges offer retention and separation of polar compounds where silica and resin stationary phases fail. (fishersci.at)
  • Obtain excellent performance for extraction of charged basic compounds with these strong cation exchanges SPE cartridges. (fishersci.at)
  • Obtain excellent normal phase extraction of polar compounds with these diol SPE well plates and individual wells. (fishersci.at)
  • Solid-phase extraction (SPE) is a sample preparation method where the compounds that are dissolved or suspended in a liquid mixture are separated from other compounds depending on their physical and chemical properties. (boekhandeldeoorsprong.nl)
  • However, according to the literature, liquid-liquid extraction (LLE) is the technique used most frequently for isolating morphinomimetic compounds (4)(5)(6)(7)(8), whereas only one method using SPE with RIA has been reported (9). (boekhandeldeoorsprong.nl)
  • Typically, this will be to extract organic compounds out of an aqueous phase and into an organic phase, but may also include extracting water-soluble impurities from an organic phase into an aqueous phase. (wikipedia.org)
  • Here, we describe a protocol for solid-phase extraction of N-linked glycopeptides and subsequent identification of N-linked glycosylation sites (N-glycosites) by tandem mass spectrometry. (nih.gov)
  • The presented analytical method is based on a solid-phase extraction (SPE) procedure to clean-up and preconcentrate the target analytes from the urine and semen samples followed by liquid chromatographyâ€"tandem mass spectrometry (LC-MS/MS) detection. (ebscohost.com)
  • Validation of an Automated Solid-Phase Extraction Method for the Analysis of 23 Opioids, Cocaine, and Metabolites in Urine with Ultra-Performance Liquid Chromatographyâ€"Tandem Mass Spectrometry. (ebscohost.com)
  • A capillary solid-phase extraction (SPE) system has been coupled directly to electrospray tandem mass spectrometry for quantification of free tolterodine and metabolite concentrations in plasma. (diva-portal.org)
  • A selection of solid phase extraction cartridges, available in many sizes, shapes, and types of stationary phase. (wikipedia.org)
  • Porvair Sciences announce Microlute™ CP - a new generation of Solid Phase Extraction (SPE) 96-well microplates and cartridges that take SPE to a new level of performance by enhancing the reproducibility of analyte extraction and recovery from biological, environmental and chemical samples. (news-medical.net)
  • Porvair Sciences launches Microlute CP, a range of Solid Phase Extraction (SPE) 96-well microplates and cartridges designed to enhance the reproducibility of analyte extraction and recovery from biological, environmental and chemical samples. (laboratorytalk.com)
  • For further information about the Microlute CP range of Solid Phase Extraction (SPE) 96-well microplates and cartridges please CLICK HERE or contact Porvair Sciences Ltd on +44-1978-661144 / [email protected] (laboratorytalk.com)
  • Aqueous samples were extracted using mixed-phase cation-exchange disk cartridges that were subsequently eluted by ammonia solution in methanol. (nih.gov)
  • Many Matrix Solid Phase Dispersion (MSPD) methods require specific cartridges and other clean-up materials in order to achieve proper extraction. (hindawi.com)
  • Clean-up of some organochlorine and pyrethroid insecticides by automated solid-phase extraction cartridges coupled to capillary GC-ECD. (rivm.nl)
  • Solid phase extraction (SPE) is a much faster purification method if optimized and it can be done with the standard cartridges as well as 96-well plates, that allow many samples to efficiently be run at the same time. (essays.se)
  • Compared with previously published methods, the developed IAC-HPLC method removed analytical interferences from swine serum in one quick and easy step, and eliminated steps of extraction with organic solvent and/or pre-purification using SPE cartridges. (ovid.com)
  • Implementation of a mixed-mode solid-phase extraction Sep-pak C_(18) and GCC-SAX-PSA SPE cartridges was discussed as a promising approach for wheat clean-up. (cnki.com.cn)
  • The performance of the Silia PrepX SCX polymeric SPE cartridges for the solid phase extraction of carbendazim in orange juice is presented following the link below. (silicycle.com)
  • This document describes a method for the extraction of Oil and Grease from water samples using SiliCycle Silia Prep ™ C18 SPE cartridges. (silicycle.com)
  • Different commercial SPE cartridges were tested for sample extraction, clean-up and preconcentration. (semanticscholar.org)
  • These SPE cartridges are compatible with the extracts generated during mycotoxin extractions and can be stored at room temperature. (foodsafetytech.com)
  • Use this flexible vacuum manifold to process samples using both solid phase extraction (SPE) cartridges and 96 well plates. (fishersci.at)
  • METHODS: 74 Pesticides were extracted by accelerated solvent extraction, and then purified with gel permeation chromatography (GPC) and solid phase extraction (SPE) cartridges. (boekhandeldeoorsprong.nl)
  • Obtain excellent performance for extraction of carboxylic and other weak acids with these strong anion exchanger SPE cartridges. (fishersci.se)
  • The extraction efficiency of different SPE cartridges was tested and the simultaneous extraction of pharmaceuticals was successfully accomplished using hydrophilic-lipophilic-balanced reversed phase Waters ® Oasis HLB cartridge (200 mg/ 6 mL) at pH 3. (preprints.org)
  • Ion exchange sorbents separate analytes based on electrostatic interactions between the analyte of interest and the positively or negatively charged groups on the stationary phase. (wikipedia.org)
  • This article evaluates the use of commercially available cyanopropyl and octadecyl sorbents for the extraction of basic drugs from breast milk. (nih.gov)
  • The polymer that had the highest binding selectivity and ability was used as solid-phase extraction (SPE) sorbents for direct extraction of BPA from different biological and. (ebscohost.com)
  • Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction. (ebscohost.com)
  • 27 phenols including mono- di-, tri-, tetra- and pentachlorophenols, mono- and dinitrophenols, mono- and dinitrocresols and dimethylnitrophenols have been extracted from aqueous samples by solid phase extraction using both modified silica gel (C18) and XAD resin-ad-sorbents and determined by gas chromatography (GC) using capillary columns with specially desactivated weakly polar phases. (fraunhofer.de)
  • Cerqueira MBR, Caldas SS, Primel EG (2014) New sorbents in dispersive solid phase extraction step of quick, easy, cheap, effective, rugged, and safe for the extraction of organic contaminants in drinking water treatment sludge. (springer.com)
  • This article discusses the preparation of different polymeric sorbents for solid phase extraction. (edu.pl)
  • The drawback to using the immunoaffinity sorbents in the lab is that they are not compatible with the mycotoxin extraction solvent. (foodsafetytech.com)
  • For more complex needs, Bond Elut solid phase extraction (SPE) products feature sorbents with phases for different analyte extraction and cleanup needs. (boekhandeldeoorsprong.nl)
  • First, the cartridge is equilibrated with a non-polar or slightly polar solvent, which wets the surface and penetrates the bonded phase. (wikipedia.org)
  • As the sample passes through the stationary phase, the polar analytes in the sample will interact and retain on the polar sorbent while the solvent, and other non-polar impurities pass through the cartridge. (wikipedia.org)
  • This stationary phase will adsorb polar molecules which can be collected with a more polar solvent. (wikipedia.org)
  • The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase. (wikipedia.org)
  • We determined the appropriate extraction solvent and its flow rate. (biomedsearch.com)
  • The principle of solid phase extraction: an aqueous sample (e.g. fruit juice, wine or body fluid) is passed through a conditioned and equilibrated SPE cartridge, washed, dried and finally eluted with a small volume of organic solvent for further measurement. (bruker.com)
  • It gives highly efficient extraction of the particles with less usage of solvent and giving accurate results. (pharmiweb.com)
  • The SOLA macro-porous structure is designed for robust, reproducible results with consistent sample and solvent flow through the SPE stationary phase, which can mitigate blockages caused by viscous biological samples. (technologynetworks.com)
  • Pressurized liquid extraction (PLE) coupled with solid-phase extraction (SPE) was used to reduce sample preparation time, reduce solvent consumption to one-fifth of that required using dichloromethane-based Soxhlet extraction, and to minimize background interferences for full scan GC/MS analysis. (usgs.gov)
  • Under optimized extraction parameters (effects of strontium-titanium dosage, desorbing solvent, adsorbent amount, solvent and sample volumes, adsorption and desorption times, salt percentage, and pH value) and matrix-matched calibration, a decent linear dynamic range was obtained 1-1000 ng mL−1 for all three hormones with acceptable relative standard deviation percentages of 1.49-2.31. (deepdyve.com)
  • Extraction of mycotoxins from samples is conducted by mixing the ground sample with the mixture of organic solvent and water, such as acetonitrile:water (80:20). (foodsafetytech.com)
  • The term washing may also be used to refer to an extraction in which impurities are extracted from the solvent containing the desired compound. (wikipedia.org)
  • After extraction is complete the solvent can be removed and the desired product collected. (wikipedia.org)
  • Magnetic solid phase extraction based on a 3D reduced graphene oxide composite coupled with spectrofluorometry was proposed to determine the presence of trace rhodamine B in chili powder and tap water. (rsc.org)
  • A method is presented for magnetic solid phase extraction (MSPE) of tetracyclines in milk samples. (edu.mx)
  • Preparation of highly fluorinated and boron-rich adsorbent for magnetic solid-phase extraction of fluoroquinolones in water and milk samples. (americanelements.com)
  • According to the molecular properties of FQs, a new highly fluorinated and boron-rich adsorbent (FBA) was synthesized and employed as the extraction phase of magnetic solid-phase extraction (MSPE). (americanelements.com)
  • SOLAµ SPE products are available in 96-well-plate format and include: SOLAµ HRP (reverse phase), SOLAµ SCX (mixed-mode strong cation exchange), SOLAµ SAX (mixed-mode strong anion exchange), SOLAµ WCX (mixed-mode weak cation exchange) and SOLAµ WAX (mixed-mode weak anion exchange) stationary phases. (technologynetworks.com)
  • A highly selective strong anion exchange - solid phase extraction (SAX-SPE) method was used (in-situ) for vanadium speciation analysis of groundwater samples from La Pampa - LP (General San Martin and Eduardo Castex) and Buenos Aires - BA (San German) provinces in Argentina. (surrey.ac.uk)
  • The results showed that the novel adsorbent could be used as an alternative material for hormone extraction in different biological fluids. (deepdyve.com)
  • A novel sol-gel hybrid methyltrimethoxysilane-tetraethoxysilane (MTMOS-TEOS) was produced and applied as sorbent for solid phase extraction (SPE). (unboundmedicine.com)
  • This unique design enhances the flow-through of samples to maximize interactions between analytes and the solid phase to deliver an unmatched reproducible SPE method that excels in performance, cleanliness and sensitivity. (news-medical.net)
  • The established routine analysis method employs Solid-phase Extraction with Absorption Spectrophotometry where the absorbance is measured of complex formed by the reaction of the non-ionic surfactant-Co(II) with a coloring reagent 4-(2-pyridylazo) resorcinol (PAR). (jascoinc.com)
  • 1.1 This test method describes a solid phase extraction (SPE) procedure to separate 99 Tc from environmental water (non-process-related or effluent water samples). (astm.org)
  • The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns operated in parallel for alternating trapping, washing and elution into a tandem quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) MS/MS mode. (rti.org)
  • Under the optimum extraction conditions, the method using the sol-gel organic-inorganic hybrid MTMOS-TEOS as SPE sorbent showed good linearity in the range of 0.001-1 μg L(-1), good repeatability (RSD 2.1-3.1%, n=5), low limits of detection at S/N=3 (0.5-0.9 pg mL(-1)) and limit of quantification (1-3 pg mL(-1), S/N=10). (unboundmedicine.com)
  • The current analytical method for urinary alkoxyacetic acid is liquid-liquid phase extraction. (biomedsearch.com)
  • But the liquid-liquid phase extraction method needs a more complex pre-treatment process and has a low recovery rate. (biomedsearch.com)
  • Finally we developed a convenient solid phase extraction method for the analysis of urine cellosolve metabolites. (biomedsearch.com)
  • LC optimization, either by gradient or mobile phase manipulation, or by alternative column selection can solve this problem for some analytes, but not always, and requires additional method development. (waters.com)
  • One group of researchers stated that "…if a more elegant solid-phase extraction (SPE) method…could be developed, an effective cleanup could possibly still be achieved. (waters.com)
  • The matrix solid phase dispersion (MSPD) method is an extraction method identified by the total disruption of the sample through the use of an appropriate bonded phase or other solid support material such as octadecylsilyl (ODS-) derivatized silica (C18 silica) being ground with the sample. (hindawi.com)
  • The method oxidizes the carbohydrates in glycopeptides into aldehydes, which can be immobilized on a solid support. (nih.gov)
  • The method involves the extraction and clean-up by silica based magnetic support dispersion on non-pretreated milk samples, followed by the magnetic isolation and desorption of the analytes by acidified methanol. (edu.mx)
  • With the use of isotope internal standards ( 15 N 5 -N7-MeG and 15 N 5 -N7-EtG) and on-line SPE (solid-phase extraction), the detection limit of this method was estimated as 0.42 fmol and 0.17 fmol for N7-MeG and N7-EtG respectively. (portlandpress.com)
  • Designed for use in all QuEChERS methods for pesticides in fruits and vegetables, including the original unbuffered method, AOAC 2007.01, and EN 15662. (restek.com)
  • The method uses a chemically modified styrene-divinylbenzene resin-based solid-phase extraction (SPE) cartridge for analyte isolation and concentration. (usgs.gov)
  • In this method, 1-liter water samples are first filtered, either in the field or in the laboratory, using a 0.7-micrometer (μm) nominal pore size glass-fiber filter to remove suspended solids. (usgs.gov)
  • A sensitive and rapid method for the simultaneous analysis of artificial sweeteners, aspartame, acesulfame, cyclamate, saccharine, and phenylalanine in water samples using solid-phase and large-volume sample stacking-capillary electrophoresis (SPE-LVSS-CE) has been developed. (springer.com)
  • A method for the confirmation and identification of drugs of misuse in urine using solid phase extraction and gas-liquid chromatography with mass spectrometry. (bmj.com)
  • Methods] The method involved an initial extraction of the wheat with acetonitrile-water. (cnki.com.cn)
  • This is a recommended general method using the Silia Prep Reversed-Phase C18. (silicycle.com)
  • Is Solid Phase Microextraction (SPME) an appropriate method for extraction of volatile oxidation products from complex food systems. (dtu.dk)
  • The extraction efficiency obtained with the SPME method can be affected by different factors such as fiber type, stirring of sample versus not stirring, extraction temperature and time. (dtu.dk)
  • Development and application of a method for the analysis of N-acylhomoserine lactones by solid-phase extraction and ultra high pressure liquid chromatography. (semanticscholar.org)
  • Development of an extraction method and LC-MS analysis for N-acylated-l-homoserine lactones (AHLs) in wastewater treatment biofilms. (semanticscholar.org)
  • The US EPA developed method 521 to measure seven nitrosamines in drinking water using solid phase extraction (SPE) and GC-MS-MS with large volume injection and chemical ionization for sensitive analysis. (spectroscopyonline.com)
  • An ion trap or triple quadrupole MS is used for MS-MS. The purpose of this application note is to demonstrate that in addition to modern measurement technology the addition of automated solid phase extraction can improve method performance (1). (spectroscopyonline.com)
  • D.D. Carlton Jr. and K.A. Schug, "Evaluation of Automated Solid-Phase Extraction for Nitrosamines Using US EPA Method 521," AN1001504_01, www.horizontechinc.com (2015). (spectroscopyonline.com)
  • EPA method 625.1, an updated version of method 625 has been recently published (by Lemuel Walker, Office of Science and Technology, US EPA) allowing for the use of solid phase extraction (SPE) as an alternative sample preparation technique, if the quality control (QC) acceptance criteria are met. (unitedchem.com)
  • A rapid solid-phase extraction-capillary zone electrophoresis (CZE) method for determining 2,4-dichlorophenoxyacetic acid, 4-(2,4- dichlorophenoxy) butyric acid, and 2,4,5-trichlorophenoxyacetic acid in real water samples is described. (nus.edu.sg)
  • However, when compared to other sample preparation techniques such as protein precipitation, liquid-liquid extraction (LLE), or QuEChERS (quick, easy, cheap, effective, rugged, and safe), SPE can be considerably more time consuming and often requires a much greater effort toward method development. (boekhandeldeoorsprong.nl)
  • M.J.M. Wells, 2000, "Essential guides to method development in solid-phase extraction," in Encyclopedia of Separation Science, Vol. 10 (I.D. Wilson, E.R. Adlard, M. Cooke, and C.F. Poole, eds. (wikipedia.org)
  • Cleanup methodologies often include the use of phase extraction (SPE). (foodsafetytech.com)
  • A new adaptation based on matrix solid phase dispersion of tissue for the subsequent isolation of polycyclic aromatic hydrocarbons was developed and used for extractions of Gulf menhaden caught during the summer of 2011. (hindawi.com)
  • Actively developing "unconventional" methods for the solid-phase extraction (SPE) of organic substances, for example, solid-phase microextraction, solid-phase dynamic extraction, dispersive solid-phase extraction, matrix solid-phase dispersion, microextraction in a packed syringe, etc., are characterized. (boekhandeldeoorsprong.nl)
  • The separation of three selective serotonin reuptake inhibitors (SSRIs) by capillary electrophoresis (CE) with fully integrated solid-phase extraction (SPE) is described. (edu.au)
  • Thabano, JE and Breadmore, MC and Hutchinson, JP and Johns, CA and Haddad, PR, Silica nanoparticle-templated methacrylic acid monoliths for in-line solid-phase extraction-capillary electrophoresis of basic analytes, Journal of Chromatography A, 1216, (25) pp. 4933-4940. (edu.au)
  • The resulting increase in ion-exchange capacity has proven to be useful for the preconcentration and separation of neurotransmitters by in-line solid-phase extraction capillary electrophoresis. (edu.au)
  • In the sweeping via solid phase extraction using the titania nanoparticles, a phosphopeptide solution is injected as a long plug into a capillary filled with an acidic background solution. (go.jp)
  • The 7800-AP is an extremely flexible, fully automated sample purification/preparation system combining advanced robotics, solid phase extraction (SPE), HPLC separation, and collection to deliver purified, concentrated fractions into standard NMR tubes ready for analysis. (environmental-expert.com)
  • The results well evidence that the introduced FBA/MSPE is a promising extraction technology for FQs, and the established FBA/MSPE-HPLC/DAD approach is suitable to measure low concentrations of FQs in water and milk samples. (americanelements.com)
  • Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. (wikipedia.org)
  • The porous properties of Microlute CP allows for consistent and controlled flow of samples throughout the filter leading to overall better extraction and recovery performance. (news-medical.net)
  • Twenty drugs were selected from different pharmacological groups (beta-blocking agents, antidepressants, anxiolytic sedatives and neuroleptics, antihistamines, alkaloids and an anthelmintic) and subjected to a general solid-phase extraction (SPE) procedure described earlier for plasma samples. (nih.gov)
  • The authors described a new application of amino-functionalized KIT-6 for dispersive ultrasonication-assisted micro solid phase extraction of hippuric acid (HA) and methyl hippuric acid (MHA) from human urine and water samples. (springer.com)
  • This was either added to plasma samples prior to extraction to achieve plasma concentrations of 200 ng/mL, or used to post-spike blank extracted plasma eluates for recovery calculations. (waters.com)
  • Extracted" samples were prepared by adding either 200 ng/mL basic drug mix or 200 ng/mL basic drug mix + 5 mg/mL PEG 400 to plasma prior to extraction. (waters.com)
  • For analysis of PEG removal, samples were diluted 1:200 in mobile phase to avoid contaminating the mass spectrometer with high concentrations of PEG 400. (waters.com)
  • The thesis focuses on the development of miniaturised solid phase extraction (SPE) technologies for the rapid and effective processing of complex biological samples prior to mass spectrometry (MS) analysis. (edu.au)
  • A selective on-column molecularly imprinted solid-phase extraction (MISPE) for ethynylestradiol (EE2) from water samples was developed. (ebscohost.com)
  • Development of a selective molecularly imprinted polymer-based solid-phase extraction for indomethacin from water samples. (ebscohost.com)
  • Selective solid-phase extraction of bisphenol A using molecularly imprinted polymers and its application to biological and environmental samples. (ebscohost.com)
  • The MIP extraction sorbent synthesized in this paper deals with these challenges by selectively pre-concentrating AFB1 from real Tsabana samples, successfully achieving a pre-concentration factor of 5 and therefore significantly increasing ABF1 signal intensity for easier detection. (degruyter.com)
  • 3 This application note will detail the second approach using SPE extraction to enrich water samples with analysis performed on Waters Xevo TQ-S micro. (waters.com)
  • To resolve this problem, packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D 2 (D 2 ) and vitamin D 3 (D 3 ) in dairy products. (scielo.br)
  • Effective extraction is an indispensable procedure for the sensitive analysis of fluoroquinolones (FQs) in complex samples. (americanelements.com)
  • This methodology discusses the use of organic solvents in sample preparation to optimize extraction recovery and minimize the hematocrit effect on the analysis of VAMS dried blood samples. (bioanalysis-zone.com)
  • SPE with the usage of Oasis MCX strong cation-exchange mixed-mode polymeric sorbent was chosen for pharmaceuticals extraction from environmental samples. (hud.ac.uk)
  • Modern Extraction Techniques for Pesticide Residues Determinatio n in Plant and Soil Samples 223 modifiers. (boekhandeldeoorsprong.nl)
  • It is an extractive technique, a solid-liquid extractive technique-taking advantage of large differences in the Keq, or Equilibrium constant , of mixture components between the solid phase and the mobile phase resulting, for a well-designed and executed separation, in a bulk separation of one or more of the mixture components so that it is significantly enriched as a result of the rapid extractive procedure. (wikipedia.org)
  • In this paper, a separation procedure combining molecularly imprinted-solid phase extraction (MI-SPE) was developed for the isolation of melamine. (ebscohost.com)
  • Alterations in the extraction procedure resulted in similar purity or lower. (essays.se)
  • Solid-Phase Extraction Procedure for Ethyl Glucuronide in Urine. (ebscohost.com)
  • The article reports on the result of the study which determines the presence of ethanol metabolite ethyl glucuronide (EtG) in human urine through solid-phase extraction (SEP) procedure. (ebscohost.com)
  • During laboratory analysis of mycotoxins, the sample preparation procedure typically includes extraction, purification and concentration steps. (foodsafetytech.com)
  • To meet these needs, Thermo Fisher Scientific has introduced Thermo Scientific SOLAµ solid phase extraction (SPE) micro elution plates. (technologynetworks.com)
  • In this example, a protocol developed at the Meyer Children's Hospital, Mass Spectrometry, Clinical Chemistry and Pharmacology Laboratory (Florence, Italy) was used to measure the enzymatic activity in dried blood spots (DBS) using an online solid phase extraction (SPE) - liquid chromatograph - tandem mass spectrometer (LCMS-8050) system. (shimadzu.com)
  • Field-Based Application of Developed Solid Phase Extraction with Inductively Coupled Plasma Mass Spectrometry for Vanadium Speciation Analysis of Groundwaters from Argentina. (surrey.ac.uk)
  • Critical parameters such as pH, flow rate, sorption capacity, breakthrough studies, distribution coefficient, preconcentration factor, concentration of eluting agents responsible for quantitative extraction of metal ions were optimized. (scirp.org)
  • The new non-covalent molecularly imprinted polymer (MIP) prepared in aqueous environment was used as a sorbent in solid-phase extraction (SPE) to selectively extract a group of naphthalene mono- and disulfonates. (strath.ac.uk)
  • Solid Phase Extraction Column Market estimates have also been provided for the historical years 2015 & 2016 along with forecast for the period from 2017 - 2025. (prismcoalition.org)
  • The global Solid Phase Extraction (SPE) Consumables market is valued at XX million USD in 2016 and is expected to reach XX million USD by the end of 2022, growing at a CAGR of XX% between 2016 and 2022. (rnrmarketresearch.com)
  • May 11, 2016· A tutorial demonstrating the solid phase extraction techniques utilized in the laboratory of Dr. Mei-Ratliff at Western Connecticut State University. (boekhandeldeoorsprong.nl)
  • Solid phase extraction can be used to isolate analytes of interest from a wide variety of matrices, including urine, blood, water, beverages, soil, and animal tissue. (wikipedia.org)
  • The result is that either the desired analytes of interest or undesired impurities in the sample are retained on the stationary phase. (wikipedia.org)
  • The portion that passes through the stationary phase is collected or discarded, depending on whether it contains the desired analytes or undesired impurities. (wikipedia.org)
  • If the portion retained on the stationary phase includes the desired analytes, they can then be removed from the stationary phase for collection in an additional step, in which the stationary phase is rinsed with an appropriate eluent . (wikipedia.org)
  • Reversed phase SPE separates analytes based on their polarity. (wikipedia.org)
  • It is possible to have an incomplete recovery of the analytes by SPE caused by incomplete extraction or elution. (wikipedia.org)
  • In the case of an incomplete extraction, the analytes do not have enough affinity for the stationary phase and part of them will remain in the permeate. (wikipedia.org)
  • Basic analytes are bound to the sorbent by strong cation-exchange, and non-ionic excipients such as PEG, binds via a reverse phase mechanism, and can be selectively removed before analyte elution. (waters.com)
  • The wide range of available media accommodates the extraction of a variety of analytes including pesticides, herbicides, carbonyls, and dioxins in compliance with 500 Series, 600 Series and 8000 Series EPA Methods. (labmate-online.com)
  • Solid-phase microextraction, or SPME, is a solid phase extraction sampling technique that involves the use of a fiber coated with an extracting phase, that can be a liquid or a solid, which extracts different kinds of analytes (including both volatile and non-volatile) from different kinds of media, that can be in liquid or gas phase. (boekhandeldeoorsprong.nl)
  • Avantor BAKERBOND spe Octadecyl (C18) Disposable Extraction Columns, are designed with standard geometries to adapt to most popular automated liquid handling systems for extracting non-ionic, non-polar to moderately polar analytes. (fishersci.se)
  • A silica monolith was developed as a solid-phase extraction sorbent for the extraction and separation of toxaphene congeners from soil extraction solutions, prior to their measurement by gas chromatography-mass spectroscopy. (springer.com)
  • It has been shown previously that commercially available solid phase extraction (SPE) columns release contaminants such as palmitic acid and stearic acid during separation of lipid classes. (chalmers.se)
  • Extraction in chemistry is a separation process consisting in the separation of a substance from a matrix. (wikipedia.org)
  • The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the advantage to require low amount of serum (1 mL) without tedious pre-treatment. (mdpi.com)
  • Novel dual-template molecularly imprinted polymers (dt-MIPs) were prepared by simple and facile precipitation polymerization using norfloxacin (NOR) and enrofloxacin (ENR) as templates for simultaneous selective recognition and extraction of the two fluoroquinolones (FQs). (rsc.org)
  • Definiton of Solid-Phase Extraction SPE is a sample preparation technology that uses solid particle, chromatographic packing material, usually contained in a cartridge type device, to chemically separate the different components of a sample. (boekhandeldeoorsprong.nl)
  • This polymeric phase is also highly stable in organic solvents. (silicycle.com)
  • This technique requires multiple extractions at 2 different sample pHs and consumes large amounts of organic solvents. (unitedchem.com)
  • With SPE, many of the problems associated with liquid/liquid extraction can be prevented, such as incomplete phase separations, less-than-quantitative recoveries, use of expe nsive, breakable specialty glassware, and disposal of large quantities of organic solvents. (boekhandeldeoorsprong.nl)
  • PerkinElmer Porcine Detection Kits - En. (selectscience.net)
  • It was then compared to the solid phase extraction technique in terms of the extraction efficiency, enrichment factor, detection limit and selectivity in wastewater. (scielo.org.za)
  • New Triple-Phase SPME Fibres and Arrows Quickly Extr. (labmate-online.com)
  • A range of different extraction methods are available such as static headspace, dynamic headspace and SPME. (dtu.dk)
  • Apr 15, 2008· Solid phase microextraction (SPME) -GC/ITMS and SPE-GC/ITMS methods were developed and optimized to determine semivolatile organic pollutants (SVOCs) in groundwater and treated drinking water in New Jersey (10-12). (boekhandeldeoorsprong.nl)
  • article{d5031b87-948e-4452-ac43-98adc0776325, abstract = {Technology that facilitates rapid investigation of solid phase extraction protocols using very small amounts of sorbent can save both time and money. (lu.se)
  • SPE uses the affinity of solutes dissolved or suspended in a liquid (known as the mobile phase ) for a solid through which the sample is passed (known as the stationary phase ) to separate a mixture into desired and undesired components. (wikipedia.org)
  • For ion exchange to occur, both the stationary phase and sample must be at a pH where both are charged. (wikipedia.org)
  • Solid phase extraction is a form of digital (step-wise) chromatography designed to extract, partition, and/or adsorb one or more components from a liquid phase (sample) onto stationary phase (sorbent or resin). (sigmaaldrich.com)
  • Through over 150 full color diagrams and straight forward text, readers will understand the power and usefulness of solid-phase extraction and how it helps solve sample preparation challenges. (wiley.com)
  • The common sample preparation methods involve saponification, 10 derivatisation, 11 liquid-liquid extraction (LLE), 12 and solid phase extraction (SPE). (scielo.br)
  • The growth of the solid phase extraction market is driven by the extensive usage in clinical, industrial, biological, food and beverages sample, marine industry, and others. (pharmiweb.com)
  • Furthermore, the main advantage of any type of solid phase extraction is that the solution or the sample can be stored for a longer period of time without any change in its concentration or identity. (pharmiweb.com)
  • The low extraction efficiency in the passive sampler supports the idea that it is not an exhaustive extraction technique and does not disturb the chemical equilibrium of the sample. (scielo.org.za)
  • The LVSPE thus proved applicable for onsite extraction of sufficient amounts of water to investigate water quality thoroughly by means of chemical analysis and effect-based tools without the common limitations due to small sample volumes. (uio.no)
  • In this installment of Robert MacNeill's (Envigo) column, Robert proposes the possibility of using solid-phase extraction for peptide sample preparation accommodating the polar and charged nature associated with peptides. (bioanalysis-zone.com)
  • Solid-phase extraction is designed for rapid sample preparation and purification prior to chromatographic analysis. (boekhandeldeoorsprong.nl)
  • Solid phase extraction (SPE) is an increasingly useful sample preparation technique. (boekhandeldeoorsprong.nl)
  • Innovative Thermo Scientific™ SOLA™ Solid Phase Extraction Plates deliver clean, highly reproducible sample extracts with lower elution volumes-for greater sensitivity, reliability and cost savings. (fishersci.se)
  • A solid sample containing the desired compound along with impurities is placed in the thimble. (wikipedia.org)
  • Arabi M, Ghaedi M, Ostovan A (2017) Water compatible molecularly imprinted nanoparticles as a restricted access material for extraction of hippuric acid, a biological indicator of toluene exposure, from human urine. (springer.com)
  • To avoid logistic and technical issues related to the storage and transport of large volumes of water, sampling would benefit greatly from onsite extraction. (uio.no)
  • This study presents a novel onsite large volume solid phase extraction (LVSPE) device tailored to fulfill the requirements for the successful effect-based and chemical screening of water resources and complies with available international standards for automated sampling devices. (uio.no)
  • Through the development of a solid phase extraction (SPE) system different fractions of smoky water were tested for their effects on germination enhancement. (edu.au)
  • Boiling tea leaves in water extracts the tannins, theobromine, and caffeine out of the leaves and into the water, as an example of a solid-liquid extraction. (wikipedia.org)
  • Resulting polymers were characterized through scanning electron microscope (SEM) and compared with Oasis HLB (Waters, MA, USA) and Strata-X (Phenomenex, Torrance, USA) on the basis of extraction performance, recovery efficiency and loading capacity. (edu.pl)
  • Granted many of the adsorbents/materials are the same as in chromatographic methods, and when these materials are packed into long columns-such that the number of theoretical plates increases by orders of magnitude as a result-the same materials result in chromatographic separations of components with even small difference is their Keq between phases. (wikipedia.org)
  • Unlike traditional loose-packed SPE methods, Microlute CP uses a hybrid structure, a solid interconnected network of evenly distributed pores combined with the retentive media. (news-medical.net)
  • Ideal for original unbuffered, AOAC (2007.01), and European (EN 15662) QuEChERS methods. (restek.com)
  • The new methods include a chemical derivatization with 3-iodobenzyl bromide, followed by novel purification approaches using supported liquid extraction and mixed-mode cation-exchange solid-phase extraction (SPE) for screening and confirmatory purposes, respectively. (deepdyve.com)
  • Clean-up methods, using either immunoaffinity chromatography (IAC) or solid phase extraction (SPE), were compared in order to determine the free form of DON or DOM-1 and the sum amount (free form plus glucuronide conjugated form of DON or DOM-1), respectively, in swine serum. (ovid.com)
  • Apr 26, 2018· The increase of testing drugs of abuse radically defines the workload for many laboratories, requiring testing methods to be accurate, reliable, and quick, which is where In-Well Hydrolysis and SPE (solid phase extraction) come in. (boekhandeldeoorsprong.nl)
  • Among different extraction methods currently used in pharmaceutics, the use of solid-phase extraction (SPE) has grown dramatically. (boekhandeldeoorsprong.nl)
  • Currently, ISO 25101 covers the extraction and analysis of only PFOA and PFOS. (waters.com)
  • Research Corridor has published a new research study titled "Solid Phase Extraction Column Market - Growth, Share, Opportunities, Competitive Analysis and Forecast, 2017 - 2025" . (prismcoalition.org)
  • The Solid Phase Extraction Column market report studies current as well as future aspects of the Solid Phase Extraction Column Market based upon factors such as market dynamics, key ongoing trends and segmentation analysis. (prismcoalition.org)
  • Volatile secondary lipid oxidation products can be identified and quantified by GC-FID or GC-MS. An extraction step is, however, needed before GC analysis. (dtu.dk)
  • Here we describe the development of a closed loop solid phase extraction setup that allows for extraction of exuded metabolites from live copepods. (dtu.dk)
  • Further evaluation of the recovery rate and non-absorptive rate according to the mini column shape, stationary phase and recovery rate with various extracting solutions is required. (biomedsearch.com)
  • From solid phase extraction, enrichment factors of 102, 113 and 167 were obtained for 2-chlorophenol, 4-chlorophenol and 2,4-dichlorophenol, respectively. (scielo.org.za)
  • When used as a SPE sorbent for three bisphenols, P-CDP showed high extraction efficiencies, high enrichment factors, and good reusability. (springer.com)
  • The extracts were further analysed by reversed-phase liquid chromatography (RP-LC). (nih.gov)
  • The extraction efficiency of the passive sampler ranged from 4 to 10% while in solid phase extraction it was 40 to 67% for the 3 chlorophenols. (scielo.org.za)
  • In both cases, extraction efficiency was highest for 2,4-dichlorophenol. (scielo.org.za)
  • These factors can easily be controlled and optimized to obtain the highest possible extraction efficiency. (dtu.dk)
  • How analogous can solid-phase extraction and analytical liquid chromatography be? (bioanalysis-zone.com)
  • In this installment of Robert MacNeill's (Envigo) column, Robert poses the question: How analogous can solid-phase extraction and analytical liquid chromatography be? (bioanalysis-zone.com)
  • Common examples include liquid-liquid extraction, and solid phase extraction. (wikipedia.org)
  • Decaffeination of tea and coffee is also an example of an extraction, where the caffeine molecules are removed from the tea leaves or coffee beans, often utilising supercritical fluid extraction with CO2 or standard solid-liquid extraction techniques. (wikipedia.org)
  • Gunt Hamburg, 2014, Thermal Process Engineering: Liquid-liquid extraction and solid-liquid extraction, see [1], accessed 12 May 2014. (wikipedia.org)
  • This paper presents the synthesis, optimization and application of a molecularly imprinted polymer (MIP) sorbent for the selective extraction and pre-concentration of the potent toxin, aflatoxin B1 (AFB1), from the child weaning food, Tsabana (manufactured in Serowe, Botswana). (degruyter.com)
  • A stationary phase of polar functionally bonded silicas with short carbons chains frequently makes up the solid phase. (wikipedia.org)
  • A stationary phase of silicon with carbon chains is commonly used. (wikipedia.org)
  • Using a proprietary design and manufacturing technique, SOLAµ plates combine polyethylene frit materials and stationary phase materials into a uniform stationary phase. (technologynetworks.com)
  • Save time and achieve more accurate pesticide determinations using these pre-prepared dispersive SPE products for QuEChERS extractions. (fishersci.se)
  • Single-use disk holders have become more popular for high throughput laboratories as they eliminate the time required to clean disk holders between extractions. (labmate-online.com)