Cholinesterase inhibition by potato glycoalkaloids slows mivacurium metabolism. (1/8)
BACKGROUND: The duration of action for many pharmaceutical agents is dependent on their breakdown by endogenous hydrolytic enzymes. Dietary factors that interact with these enzyme systems may alter drug efficacy and time course. Cholinesterases such as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) hydrolyze and inactivate several anesthetic drugs, including cocaine, heroin, esmolol, local ester anesthetics, and neuromuscular blocking drugs. Natural glycoalkaloid toxins produced by plants of the family Solanaceae, which includes potatoes and tomatoes, inhibit both AChE and BuChE. Here the authors assess the extent to which two solanaceous glycoalkaloids (SGAs), alpha-solanine and alpha-chaconine, can alter the effects of neuromuscular blocking drugs and cholinesterase inhibitors in vivo and in vitro. METHODS: Inhibition of purified human AChE and BuChE by SGAs, neuromuscular blocking drugs, and cholinesterase inhibitors was assessed by an in vitro colorimetric cholinesterase assay. In vivo experiments were carried out using anesthetized rabbits to test whether SGAs affect recovery from mivacurium-induced paralysis. RESULTS: SGAs inhibited human BuChE at concentrations similar to those found in serum of individuals who have eaten a standard serving of potatoes. Coapplication of SGAs (30-100 nm) with neuromuscular blocking drugs and cholinesterase inhibitors produced additive cholinesterase inhibition. SGA administration to anesthetized rabbits inhibited serum cholinesterase activity and mivacurium hydrolysis. In addition, SGA prolonged the time needed for recovery from mivacurium-induced paralysis (149 +/- 12% of control; n = 12). CONCLUSIONS: These findings support the hypothesis that inhibition of endogenous enzyme systems by dietary factors can influence anesthetic drug metabolism and duration of action. Diet may contribute to the wide variation in recovery time from neuromuscular blockade seen in normal, healthy individuals. (+info)Effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells. (2/8)
AIM: To observe the effect of solanine on the membrane potential of mitochondria in HepG(2) cells and [Ca(2+)](i) in the cells, and to uncover the mechanism by which solanine induces apoptosis. METHODS: HepG(2) cells were double stained with AO/EB, and morphological changes of the cells were observed using laser confocal scanning microscopy (LCSM). HepG(2) cells were stained with TMRE, and change in the membrane potential of mitochondria in the cells were observed using LCSM. HepG(2) cells were double stained with Fluo-3/AM, and change of [Ca(2+)](i) in the cells were observed using LCSM. HepG(2) cells were double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca(2+)](i) in the cells were observed using LCSM. RESULTS: Cells in treated groups showed typical signs of apoptosis. Staining with TMRE showed that solanine could lower membrane potential; staining with Fluo-3/AM showed that solanine could increase the concentration of Ca(2+) in tumor cells; and those of double staining with TMRE and Fluo-3/AM showed that solanine could increase the concentration of Ca(2+) in the cells at the same time as it lowered the membrane potential of mitochondria. CONCLUSION: Solanine opens up the PT channels in the membrane by lowering the membrane po-tential, leading to Ca(2+) being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca(2+) in the cell, turning on the mechanism for apoptosis. (+info)alpha-Chaconine inhibits angiogenesis in vitro by reducing matrix metalloproteinase-2. (3/8)
alpha-Chaconine, a naturally occurring steroidal glycoalkaloid in potato sprouts, was found to possess anti-carcinogenic properties, such as inhibiting proliferation, migration, invasion, and inducing apoptosis of tumor cells. However, the effect of alpha-chaconine on tumor angiogenesis remains unclear. In the present study, we examined the effect of alpha-chaconine on angiogenesis in vitro. Data demonstrated that alpha-chaconine inhibited proliferation of bovine aortic endothelial cells (BAECs) in a dose-dependent manner. When treated with non-toxic doses of alpha-chaconine, cell migration, invasion and tube formation were markedly suppressed. Furthermore, alpha-chaconine reduced the expression and activity of matrix metalloproteinase-2 (MMP-2), which is involved in angiogenesis. Our biochemical assays indicated that alpha-chaconine potently suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), phosphatidylinositide-3 kinase (PI3K) and Akt, while it did not affect phosphorylation of extracellular signal regulating kinase (ERK) and p38. In addition, alpha-chaconine significantly increased the cytoplasmic level of inhibitors of kappaBalpha (IkappaBalpha) and decreased the nuclear level of nuclear factor kappa B (NF-kappaB), suggesting that alpha-chaconine could inhibit NF-kappaB activity. Furthermore, the treatment of inhibitors specific for JNK (SP600125), PI3K (LY294002) or NF-kappaB (pyrrolidine dithiocarbamate) to BAECs reduced tube formation. Taken together, the results suggested that alpha-chaconine inhibited migration, invasion and tube formation of BAECs by reducing MMP-2 activities, as well as JNK and PI3K/Akt signaling pathways and inhibition of NF-kappaB activity. These findings reveal a new therapeutic potential for alpha-chaconine on anti-angiogenic therapy. (+info)alpha-Solanine inhibits human melanoma cell migration and invasion by reducing matrix metalloproteinase-2/9 activities. (4/8)
alpha-Solanine, a naturally occurring steroidal glycoalkaloid in potato sprouts, was found to possess anti-carcinogenic properties, such as inhibiting proliferation and inducing apoptosis of tumor cells. However, the effect of alpha-solanine on cancer metastasis remains unclear. In the present study, we examined the effect of alpha-solanine on metastasis in vitro. Data demonstrated that alpha-solanine inhibited proliferation of human melanoma cell line A2058 in a dose-dependent manner. When treated with non-toxic doses of alpha-solanine, cell migration and invasion were markedly suppressed. Furthermore, alpha-solanine reduced the activity of matrix metalloproteinase-2 (MMP-2) and MMP-9, which are involved in the migration and invasion of cancer cells. Our biochemical assays indicated that alpha-solanine potently suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), phosphatidylinositide-3 kinase (PI3K) and Akt, while it did not affect phosphorylation of extracellular signal regulating kinase (ERK). In addition, alpha-solanine significantly decreased the nuclear level of nuclear factor kappa B (NF-kappaB), suggesting that alpha-solanine inhibited NF-kappaB activity. Taken together, the results suggested that alpha-solanine inhibited migration and invasion of A2058 cells by reducing MMP-2/9 activities. It also inhibited JNK and PI3K/Akt signaling pathways as well as NF-kappaB activity. These findings reveal new therapeutic potential for alpha-solanine in anti-metastatic therapy. (+info)Review of the inhibition of biological activities of food-related selected toxins by natural compounds. (5/8)
(+info)Extending the detection limit of the TDx fluorescence polarization immunoassay for benzoylecgonine in urine. (6/8)
This modified calibration method decreases from 300 to 27 micrograms/L the limit of detection for the cocaine metabolite (hydrolysis product), benzoylecgonine, by the Abbott Laboratories TDx fluorescence polarization immunoassay. For this determination we used 30 controls prepared from a single urine pool known to be negative for cocaine metabolite. Assay of 80 controls prepared from 20 different patients' urine samples yielded a limit of detection of 44 micrograms/L. To test these limits of detection, we analyzed 90 patients' urine samples known to be negative for cocaine metabolite and 74 patients' samples known to be positive for cocaine metabolite, using the TDx with our revised calibration. Results for two of the known negative samples and 96% of the samples containing cocaine in the 50 to 100 micrograms/L range fell above the 44 micrograms/L limit. The TDx showed excellent calibration stability. For 28 days during the test, the instrument was not recalibrated. During this period the day-to-day analysis of 50 micrograms/L controls produced a mean TDx response of 0.485 (SD 0.007) with a coefficient of variation of 1.5%. (+info)Feeding of potato, tomato and eggplant alkaloids affects food consumption and body and liver weights in mice. (7/8)
Reduced liver weight was used to evaluate the potential toxicity in mice of four naturally occurring steroidal glycoalkaloids: alpha-chaconine and alpha-solanine, alpha-tomatine and solasonine. Increased liver weights was used to evaluate the three corresponding steroidal aglycones: solanidine, tomatidine, and solasodine and the non-alkaloid adrenal steroid dehydroepiandrosterone (DHEA). Adult female Swiss-Webster mice were fed diets containing test compound concentrations of 0 (control), 1.2, 2.4 or 4.8 mmol/kg diet for 7, 14 or 28 d. Absolute liver weights (LW) and relative liver weights (liver weight/body weight x 100, %LW/BW) were determined at autopsy. The %LW/BW was lower than that of controls in mice fed the potato glycoalkaloid alpha-chaconine (-10%, P < or = 0.05) for 7 d with the 2.4 mmol/kg diet dose. Under these same conditions, %LW/BW was greater than that of controls in mice fed two aglycones: solanidine (27%, P < or = 0.001) and solasodine (8%, P < or = 0.01). Relative liver weight increases induced by the aglycones were determined under time and dose conditions in which differences in body weight and food consumption were not significant (2.4 mmol/kg diet for 28 d). Under these conditions, the observed %LW/BW increases relative to the controls were as follows: solanidine (32%, P < or = 0.001), solasodine (22%, P < or = 0.001) and DHEA (16%, P < or = 0.001). Solanidine, solasodine and DHEA were equally potent and were more potent than tomatidine. We also observed that the greater %LW/BW in mice fed 2.4 mmol/kg diet solasodine or solanidine for 14 d declined to near control values if they were fed control diets for another 14 d. The increase in relative liver weight induced by solanidine and solasodine is a reversible adaptive response. These findings and the apparent effects of structure on biological activity should serve as a guide for the removal of the most toxic ++compounds from plant foods. The implications of the results for food safety and health are discussed. (+info)Synthesis of gamma-chaconine and gamma-solanine are catalyzed in potato by two separate glycosyltransferases: UDP-glucose:solanidine glucosyltransferase and UDP-galactose:solanidine galactosyltransferase. (8/8)
UDP-glucose:solanidine glucosyltransferase and UDP-galactose:solanidine galactosyltransferase from cytosol of potato sprouts were partially separated by Sephadex G-100 and Q-Sepharose chromatographies, proving the existence of different glycosylation systems in biosynthesis of alpha-chaconine and alpha-solanine. (+info)Solanine is a glycoalkaloid toxin found in plants of the nightshade family, Solanaceae, which includes potatoes, tomatoes, eggplants, and peppers. It's primarily concentrated in the leaves, stems, and fruits (green potatoes and green, sprouted, or damaged potato areas), but it can also be found in lower concentrations in other parts of these plants. Solanine has a bitter taste and is produced by the plant as a defense mechanism against pests and diseases. When consumed in large amounts, solanine can cause symptoms such as gastrointestinal disturbances, nausea, diarrhea, vomiting, and neurological problems like headaches, dizziness, and confusion. In severe cases, it may lead to paralysis and even death. However, it's important to note that solanine concentrations in commonly consumed nightshade vegetables are generally low, and toxic effects are unlikely to occur from normal consumption unless the vegetables are spoiled or improperly prepared.
Solanine
Cestrum nocturnum
Tomatine
Microgreen
Solanum dulcamara
Median lethal dose
List of Puccinia species
Chaconine
Solanum nigrum
Tomato
Solanum xanti
Progesterone
Solanum aculeastrum
Solanum americanum
Plant breeding
Vladimir Prelog
Steroidal alkaloid
Solanidine
List of poisonous plants
Sprouting
Regulation of genetic engineering
Potato
Bush tomato
Solanum jamesii
Hilling
Eggplant
Solanaceae
Solanum pseudocapsicum
Glycoalkaloid
Macrobiotic diet
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Toxin called solanine5
- Green potatoes contain a natural toxin called solanine. (snopes.com)
- The potato - or, rather, green versions of it - contains a natural toxin called solanine. (snopes.com)
- This is because tomatoes naturally produce a toxin called solanine. (onteenstoday.com)
- That said, nightshade plants have high levels of a toxin called solanine . (gardeningknowhow.com)
- Green parts of potatoes contain a natural toxin called solanine and should not be eaten. (miraclegro.com)
Chemical called solanine2
- Nightshade plants contain a poisonous chemical called solanine. (thedodo.com)
- The main reason why eating a spoiled potato is so dangerous is because they contain a chemical called solanine. (beinghuman.org)
Case of solanine2
- The well known natural therapist, Andrew Weil, points out that there hasn't been a single case of solanine poisoning in the US from potatoes in the last 50 years. (health.org.au)
- In the case of solanine toxicity from tomatoes, if your dog were to experience adverse effects and require veterinary care, it's important to know if your insurance policy covers such incidents. (abestfashion.com)
Toxicity in dogs1
- The tomato plant can cause solanine toxicity in dogs, which can be fatal. (bustle.com)
Glycoalkaloid solanine2
- Potato peels, just like raw potatoes, are full of glycoalkaloid solanine that is toxic to cats. (petoftheday.com)
- All parts of the plant contain toxic glycoalkaloid solanine and may cause gastrointestinal problems if ingested. (pestweb.com)
Chaconine5
- Potatoes naturally produce solanine and chaconine, a related glycol-alkaloid, as a defence against pests. (health.org.au)
- Two tri-glycosylated alkaloids, alpha-chaconine and alpha-solanine accumulate in potato tubers. (usda.gov)
- However, the profiles of component SGAs were altered in some of the antisense lines showing large reductions in alpha-chaconine usually compensated by an increase alpha-solanine. (usda.gov)
- You shouldn't eat the sprouts because they contain solanine, chaconine, and other toxic glycoalkaloids. (healthline.com)
- Other studies have found that neural tube defects in hamsters may be caused by the solanidine triglycosides, alpha-chaconine and high-dose solanine. (family-health-information.com)
Tomatoes18
- Would the solanine content be minimised if I select only tomatoes that are very ripe? (health.org.au)
- The short answer is that solanine is at its highest levels in green tomatoes - as it is in green potatoes - and decreases to its minimum levels in dead ripe fruit. (health.org.au)
- So, yes, your best chance of avoiding irritation from solanine is to consume only perfectly-ripe tomatoes. (health.org.au)
- Most plants contain natural pesticides, and solanine is the one in tomatoes. (health.org.au)
- In potatoes, the parts which have turned green have the highest levels of solanine, which are much greater and more harmful than the solanine in tomatoes. (health.org.au)
- In tomatoes, the highest level of the solanine group of chemicals, called tomatine, occurs when they are immature and green. (health.org.au)
- The concentration can be up to 500 mg per 1 kg of fruit, according to the website https://www.ehow.com/info_8738165_solanine-ripe-vs-green-tomatoes.html. (health.org.au)
- But for cats, even the solanine in ripe tomatoes is harmful. (health.org.au)
- In most cases, tomatoes ripened on the bush have lower levels of solanine than greenhouse tomatoes because the latter have often been harvested green. (health.org.au)
- Children and adults with tomato allergies and sensitivity are the most affected by the solanine in green tomatoes. (health.org.au)
- One of the primary concerns is solanine toxicity, as tomatoes belong to the nightshade family, which contains solanine, a potentially harmful substance for dogs. (abestfashion.com)
- In this article, we will explore the topic of whether dogs can eat tomatoes, the risks associated with solanine toxicity, and how it relates to insurance considerations for pet owners. (abestfashion.com)
- While tomatoes themselves are not toxic to dogs, the plant's stems, leaves, and unripe green tomatoes contain higher levels of solanine, which can be harmful to dogs if ingested in large quantities. (abestfashion.com)
- Some dogs may have a sensitive digestive system or be more prone to solanine toxicity, while others may tolerate tomatoes without any issues. (abestfashion.com)
- Solanine is a natural toxin found in plants of the nightshade family, including tomatoes, potatoes, and eggplants. (abestfashion.com)
- Tomatoes technically contain tomatine instead of solanine, which is essentially a less toxic version of that chemical. (thedodo.com)
- The fruits of nightshade plants-potatoes, tomatoes, peppers and eggplants-generally contain very low levels of solanine, which is why they are safe to eat. (diettogo.com)
- Some people are highly sensitive to solanine and for these folks, eating tomatoes and peppers can cause swelling and pain in the joints. (diettogo.com)
Concentrations3
- High concentrations of solanine are necessary to cause death to mammals. (wikipedia.org)
- The greenish hue that should warn you away from such spuds is actually chlorophyll, but its presence indicates concentrations of solanine are present in the tuber. (snopes.com)
- Concentrations of 38 to 45 mg/100 g solanine have been found in potatoes implicated in human fatalities, compared to 3 to 66 mg/100 g in fresh, healthy potatoes. (family-health-information.com)
Leaves and stems2
- Plants, leaves and stems, contain a poison called solanine which causes severe gastrointestinal problems. (hartz.com)
- The presence of solanine in the leaves and stems of nightshade plants acts as a natural pesticide, dispatching any bugs that nibble too freely on the plant. (diettogo.com)
Amounts5
- Ingestion of solanine in moderate amounts can cause death. (wikipedia.org)
- When dogs consume large amounts of solanine, they may experience symptoms such as gastrointestinal distress, including vomiting and diarrhea. (abestfashion.com)
- At least one of these substances, solanine, is toxic to humans in large amounts. (botanicalinterests.com)
- All of these plants produce a compound called solanine that, when consumed in sufficient amounts, acts as a nerve poison. (diettogo.com)
- If a potato is green or sprouted, it's a sign that it contains solanine, a compound that is toxic even in small amounts. (losethebackpain.com)
Tubers1
- When the plant's stem, tubers, or leaves are exposed to sunlight, it stimulates the biosynthesis of solanine and other glycoalkaloids as a defense mechanism so it is not eaten. (wikipedia.org)
Tomato6
- Solanine is a glycoalkaloid poison found in species of the nightshade family within the genus Solanum, such as the potato (Solanum tuberosum), the tomato (Solanum lycopersicum), and the eggplant (Solanum melongena). (wikipedia.org)
- Potato, tomato, and eggplant glycoalkaloids like solanine have also been shown to affect active transport of sodium across cell membranes. (wikipedia.org)
- Plants like the potato and tomato constantly synthesize low levels of glycoalkaloids like solanine. (wikipedia.org)
- I eat a lot of tomato, which I know can aggravate arthritis due to the solanine content. (health.org.au)
- The softer and sweeter the ripe tomato, the less solanine it contains. (health.org.au)
- And even though you're a lot bigger than that bug, the amount of solanine in potato or tomato leaves might be enough to give you an upset stomach, which is why we don't eat those parts of the plant. (diettogo.com)
Vomiting and diarrhea1
- When ingested, solanine causes vomiting and diarrhea, slows the heart, lowers blood pressure, depresses breathing and induces coma. (vanderbilt.edu)
Potato2
- Solanine is a glycoalkaloid poison created by various plants in the genus Solanum, such as the potato plant. (wikipedia.org)
- A glycoalkaloid poison found in species of the nightshade, solanine is a nerve toxin produced in the green part of the potato (the leaves, the stem, and any green spots on the skin). (snopes.com)
Ingestion1
- Symptoms usually occur 8 to 12 hours after ingestion, but may occur as rapidly as 10 minutes after eating high-solanine foods. (wikipedia.org)
Humans3
- Some studies show a correlation between the consumption of potatoes suffering from late blight (which increases solanine and other glycoalkaloid levels) and the incidence of spina bifida in humans. (wikipedia.org)
- There are several proposed mechanisms of how solanine causes toxicity in humans, but the true mechanism of action is not well understood. (wikipedia.org)
- In humans, solanine toxicity can cause gastrointestinal upset, headache, and even neurological symptoms in severe cases. (abestfashion.com)
Diarrhea2
- This cell membrane disruption is likely the cause of many of the symptoms of solanine toxicity, including burning sensations in the mouth, nausea, vomiting, abdominal cramps, diarrhea, internal hemorrhaging, and stomach lesions. (wikipedia.org)
- The solanum glycosides, such as solanine, produce gastrointestinal disturbances including nausea, vomiting, diarrhea and hemolytic and hemorrhagic damage to the gastrointestinal tract. (family-health-information.com)
Compound1
- Solanine is a glycol-alkaloid compound and is present in all parts of the plant. (health.org.au)
Exposure3
- Experiments show that solanine exposure opens the potassium channels of mitochondria, increasing their membrane potential. (wikipedia.org)
- Under stressful pre and/or post-harvest conditions (including temperature, humidity, storage time, physical injury, and exposure to light) glycoalkaloids - solanine in particular - begin to increase to higher levels, becoming toxic and very dangerous if consumed. (tallahassee.com)
- This discoloration is called "solanine" and is caused by exposure to light. (1859oregonmagazine.com)
Solanidine2
- However, it is known that in the biosynthesis of solanine, cholesterol is first converted into the steroidal alkaloid solanidine. (wikipedia.org)
- The solanidine is then converted into solanine through a series of glycosylation reactions catalyzed by specific glycosyltransferases. (wikipedia.org)
Naturally1
- Another concern is that levels of naturally occurring toxins, such as solanine, might be increased in GM versions of plants. (cspinet.org)
Consume1
- The largest concentration of solanine in response to stress is on the surface in the peel, making it an even better defense mechanism against pests trying to consume it. (wikipedia.org)
Less toxic1
- Ingested solanine is relatively less toxic than that administered parenterally. (family-health-information.com)
Poison found1
- Solanine is a poison found in potatoes. (indianlink.com.au)
Berries1
- Solanine was first isolated in 1820 from the berries of the European black nightshade (Solanum nigrum), after which it was named. (wikipedia.org)
Compounds1
- However, under stress, such as the presence of a pest or herbivore, they increase the synthesis of compounds like solanine as a natural chemical defense. (wikipedia.org)
Levels1
- Toxicity typically occurs when people ingest potatoes containing high levels of solanine. (wikipedia.org)
Decrease2
- The gastrointestinal tract cannot efficiently absorb solanine, which helps decrease its strength to the mammal body. (wikipedia.org)
- In other words, does the solanine content decrease with the ripening of the fruit? (health.org.au)
Natural1
- Solanine has pesticidal properties, and it is one of the plant's natural defenses. (wikipedia.org)
Green3
- The green colour is chlorophyll, which is harmless, but is an indication of a high solanine level. (health.org.au)
- You are unlikely to ingest enough solanine from eating green potatoes to do you any serious harm. (snopes.com)
- Even though human fatalities due to the consumption of green potatoes have been reported periodically, proof that solanine was the causal agent has not been firmly established. (family-health-information.com)