An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.
The sum of the weight of all the atoms in a molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
The rate dynamics in chemical or physical systems.
Agents that modify interfacial tension of water; usually substances that have one lipophilic and one hydrophilic group in the molecule; includes soaps, detergents, emulsifiers, dispersing and wetting agents, and several groups of antiseptics.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Inorganic salts of sulfuric acid.
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Ion channels that specifically allow the passage of SODIUM ions. A variety of specific sodium channel subtypes are involved in serving specialized functions such as neuronal signaling, CARDIAC MUSCLE contraction, and KIDNEY function.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A naturally occurring glycosaminoglycan found mostly in the skin and in connective tissue. It differs from CHONDROITIN SULFATE A (see CHONDROITIN SULFATES) by containing IDURONIC ACID in place of glucuronic acid, its epimer, at carbon atom 5. (from Merck, 12th ed)
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Particles consisting of aggregates of molecules held loosely together by secondary bonds. The surface of micelles are usually comprised of amphiphatic compounds that are oriented in a way that minimizes the energy of interaction between the micelle and its environment. Liquids that contain large numbers of suspended micelles are referred to as EMULSIONS.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins found in any species of bacterium.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
A proteolytic enzyme obtained from Streptomyces griseus.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.
A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
A ubiquitous sodium salt that is commonly used to season food.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Long-chain polymer of glucose containing 17-20% sulfur. It has been used as an anticoagulant and also has been shown to inhibit the binding of HIV-1 to CD4-POSITIVE T-LYMPHOCYTES. It is commonly used as both an experimental and clinical laboratory reagent and has been investigated for use as an antiviral agent, in the treatment of hypolipidemia, and for the prevention of free radical damage, among other applications.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
A method which uses specific precipitation reactions to separate or collect substances from a solution.
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.
Sodium or sodium compounds used in foods or as a food. The most frequently used compounds are sodium chloride or sodium glutamate.
Inorganic and organic derivatives of sulfuric acid (H2SO4). The salts and esters of sulfuric acid are known as SULFATES and SULFURIC ACID ESTERS respectively.
The chemical and physical integrity of a pharmaceutical product.
Cetyltrimethylammonium compounds that have cationic detergent, antiseptic, and disinfectant activities. They are used in pharmaceuticals, foods, and cosmetics as preservatives; on skin, mucous membranes, etc., as antiseptics or cleansers, and also as emulsifiers. These compounds are toxic when used orally due to neuromuscular blockade.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
Established cell cultures that have the potential to propagate indefinitely.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Proteins isolated from the outer membrane of Gram-negative bacteria.
A highly caustic substance that is used to neutralize acids and make sodium salts. (From Merck Index, 11th ed)
Proteins found in any species of virus.
Chemical agents that react with SH groups. This is a chemically diverse group that is used for a variety of purposes. Among these are enzyme inhibition, enzyme reactivation or protection, and labelling.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
The methyl imidoester of suberic acid used to produce cross links in proteins. Each end of the imidoester will react with an amino group in the protein molecule to form an amidine.
Glycoproteins which have a very high polysaccharide content.
A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Antibodies produced by a single clone of cells.
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
Transport proteins that carry specific substances in the blood or across cell membranes.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Sites on an antigen that interact with specific antibodies.
The process of cleaving a chemical compound by the addition of a molecule of water.
Substances elaborated by bacteria that have antigenic activity.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of SH (thiol) groups and for reducing disulphides to dithiols.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A small colorless crystal used as an anticonvulsant, a cathartic, and an electrolyte replenisher in the treatment of pre-eclampsia and eclampsia. It causes direct inhibition of action potentials in myometrial muscle cells. Excitation and contraction are uncoupled, which decreases the frequency and force of contractions. (From AMA Drug Evaluations Annual, 1992, p1083)
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
A series of steps taken in order to conduct research.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
Chloride and mercury-containing derivatives of benzoic acid.
Organic or inorganic compounds that contain the -N3 group.
Contractile tissue that produces movement in animals.
Esters of the hypothetical imidic acids. They react with amines or amino acids to form amidines and are therefore used to modify protein structures and as cross-linking agents.
Stable sulfur atoms that have the same atomic number as the element sulfur, but differ in atomic weight. S-33, 34, and 36 are stable sulfur isotopes.
A bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine. Deoxycholic acid acts as a detergent to solubilize fats for intestinal absorption, is reabsorbed itself, and is used as a choleretic and detergent.
Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.
A subclass of IMIDES with the general structure of pyrrolidinedione. They are prepared by the distillation of ammonium succinate. They are sweet-tasting compounds that are used as chemical intermediates and plant growth stimulants.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
A class of drugs that act by inhibition of sodium influx through cell membranes. Blockade of sodium channels slows the rate and amplitude of initial rapid depolarization, reduces cell excitability, and reduces conduction velocity.
Stable sodium atoms that have the same atomic number as the element sodium, but differ in atomic weight. Na-23 is a stable sodium isotope.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Elements of limited time intervals, contributing to particular results or situations.
Organic esters of sulfuric acid.
Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
Colorless, odorless crystals that are used extensively in research laboratories for the preparation of polyacrylamide gels for electrophoresis and in organic synthesis, and polymerization. Some of its polymers are used in sewage and wastewater treatment, permanent press fabrics, and as soil conditioning agents.
A class of enzymes that catalyze the hydrolysis of phosphoglycerides or glycerophosphatidates. EC 3.1.-.
Compounds containing the -SH radical.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
A proteolytic enzyme obtained from Carica papaya. It is also the name used for a purified mixture of papain and CHYMOPAPAIN that is used as a topical enzymatic debriding agent. EC 3.4.22.2.
Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Viruses whose host is Escherichia coli.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
Usually high-molecular-weight, straight-chain primary alcohols, but can also range from as few as 4 carbons, derived from natural fats and oils, including lauryl, stearyl, oleyl, and linoleyl alcohols. They are used in pharmaceuticals, cosmetics, detergents, plastics, and lube oils and in textile manufacture. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A mucopolysaccharide constituent of chondrin. (Grant & Hackh's Chemical Dictionary, 5th ed)
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
A sulfate salt of copper. It is a potent emetic and is used as an antidote for poisoning by phosphorus. It also can be used to prevent the growth of algae.
Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A 90-amino acid peptide derived from post-translational processing of pro-opiomelanocortin (POMC) in the PITUITARY GLAND and the HYPOTHALAMUS. It is the C-terminal fragment of POMC with lipid-mobilizing activities, such as LIPOLYSIS and steroidogenesis. Depending on the species and the tissue sites, beta-LPH may be further processed to yield active peptides including GAMMA-LIPOTROPIN; BETA-MSH; and ENDORPHINS.
Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.
Organic compounds that contain the (-NH2OH) radical.
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.
A white, crystalline powder that is commonly used as a pH buffering agent, an electrolyte replenisher, systemic alkalizer and in topical cleansing solutions.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
A family of iminourea derivatives. The parent compound has been isolated from mushrooms, corn germ, rice hulls, mussels, earthworms, and turnip juice. Derivatives may have antiviral and antifungal properties.
Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.
Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
A sulfur-containing essential L-amino acid that is important in many body functions.
The functional hereditary units of BACTERIA.
A group of oxidoreductases that act on NADH or NADPH. In general, enzymes using NADH or NADPH to reduce a substrate are classified according to the reverse reaction, in which NAD+ or NADP+ is formally regarded as an acceptor. This subclass includes only those enzymes in which some other redox carrier is the acceptor. (Enzyme Nomenclature, 1992, p100) EC 1.6.

Biophysical characterization of the structure of the amino-terminal region of gp41 of HIV-1. Implications on viral fusion mechanism. (1/1923)

A peptide of 51 amino acids corresponding to the NH2-terminal region (5-55) of the glycoprotein gp41 of human immunodeficiency virus type 1 was synthesized to study its conformation and assembly. Nuclear magnetic resonance experiments indicated the sequence NH2-terminal to the leucine zipper-like domain of gp41 was induced into helix in the micellar solution, in agreement with circular dichroism data. Light scattering experiment showed that the peptide molecules self-assembled in water into trimeric structure on average. That the peptide molecules oligomerize in aqueous solution was supported by gel filtration and diffusion coefficient experiments. Molecular dynamics simulation based on the NMR data revealed a flexible region adjacent to the hydrophobic NH2 terminus of gp41. The biological significance of the present findings on the conformational flexibility and the propensity of oligomerization of the peptide may be envisioned by a proposed model for the interaction of gp41 with membranes during fusion process.  (+info)

Molecular dynamics study of substance P peptides partitioned in a sodium dodecylsulfate micelle. (2/1923)

Two neuropeptides, substance P (SP) and SP-tyrosine-8 (SP-Y8), have been studied by molecular dynamics (MD) simulation in an explicit sodium dodecylsulfate (SDS) micelle. Initially, distance restraints derived from NMR nuclear Overhauser enhancements (NOE) were incorporated in the restrained MD (RMD) during the equilibration stage of the simulation. It was shown that when SP-Y8 was initially placed in an insertion (perpendicular) configuration, the peptide equilibrated to a surface-bound (parallel) configuration in approximately 450 ps. After equilibration, the conformation and orientation of the peptides, the solvation of both the backbone and the side chain of the residues, hydrogen bonding, and the dynamics of the peptides were analyzed from trajectories obtained from the RMD or the subsequent free MD (where the NOE restraints were removed). These analyses showed that the peptide backbones of all residues are either solvated by water or are hydrogen-bonded. This is seen to be an important factor against the insertion mode of interaction. Most of the interactions come from the hydrophobic interaction between the side chains of Lys-3, Pro-4, Phe-7, Phe-8, Leu-10, and Met-11 for SP, from Lys-3, Phe-7, Leu-10, and Met-11 in SP-Y8, and the micellar interior. Significant interactions, electrostatic and hydrogen bonding, between the N-terminal residues, Arg-Pro-Lys, and the micellar headgroups were observed. These latter interactions served to affect both the structure and, especially, the flexibility, of the N-terminus. The results from simulation of the same peptides in a water/CCl4 biphasic cell were compared with the results of the present study, and the validity of using the biphasic system as an approximation for peptide-micelle or peptide-bilayer systems is discussed.  (+info)

The intracellular serpin proteinase inhibitor 6 is expressed in monocytes and granulocytes and is a potent inhibitor of the azurophilic granule protease, cathepsin G. (3/1923)

The monocyte and granulocyte azurophilic granule proteinases elastase, proteinase 3, and cathepsin G are implicated in acute and chronic diseases thought to result from an imbalance between the secreted proteinase(s) and circulating serpins such as alpha1-proteinase inhibitor and alpha1-antichymotrypsin. We show here that the intracellular serpin, proteinase inhibitor 6 (PI-6), is present in monocytes, granulocytes, and myelomonocytic cell lines. In extracts from these cells, PI-6 bound an endogenous membrane-associated serine proteinase to form an sodium dodecyl sulfate (SDS)-stable complex. Using antibodies to urokinase, elastase, proteinase 3, or cathepsin G, we demonstrated that the complex contains cathepsin G. Native cathepsin G and recombinant PI-6 formed an SDS-stable complex in vitro similar in size to that observed in the extracts. Further kinetic analysis demonstrated that cathepsin G and PI-6 rapidly form a tight 1:1 complex (ka = 6.8 +/- 0.2 x 10(6) mol/L-1s-1 at 17 degrees C; Ki = 9.2 +/- 0.04 x 10(-10) mol/L). We propose that PI-6 complements alpha1-proteinase inhibitor and alpha1-antichymotrypsin (which control extracellular proteolysis) by neutralizing cathepsin G that leaks into the cytoplasm of monocytes or granulocytes during biosynthesis or phagocytosis. Control of intracellular cathepsin G may be particularly important, because it has recently been shown to activate the proapoptotic proteinase, caspase-7.  (+info)

Single channel analysis of recombinant major outer membrane protein porins from Chlamydia psittaci and Chlamydia pneumoniae. (4/1923)

We recently demonstrated that the major outer membrane protein of Chlamydia psittaci, the primary vaccine candidate for combating chlamydial infections, functions as a porin-like ion channel. In this study, we have cloned, expressed and functionally reconstituted recombinant major outer membrane proteins from C. psittaci and Chlamydia pneumoniae and analysed them at the single channel level. Both form porin-like ion channels that are functionally similar to those formed by native C. psittaci major outer membrane protein. Also, like the native channels, recombinant C. psittaci channels are modified by a native major outer membrane protein-specific monoclonal antibody. This is the first time that native function has been demonstrated for recombinant chlamydial major outer membrane proteins. Future bilayer reconstitution will provide a strategy for detailed structure/function studies of this new subclass of bacterial porins and the work also has important implications for successful protein refolding and the development of improved subunit vaccines.  (+info)

Use of PCR and sodium dodecyl sulfate-polyacrylamide gel electrophoresis techniques for differentiation of Prevotella intermedia sensu stricto and Prevotella nigrescens. (5/1923)

Primers were designed from 16S rRNA sequences of Prevotella intermedia sensu stricto and Prevotella nigrescens and were used to discriminate these two species by PCR. The results were compared with those from the PCR technique using primers designed from arbitrarily primed PCR products by Guillot and Mouton (E. Guillot and C. Mouton, J. Clin. Microbiol. 35:1876-1882, 1997). The specificities of both assays were studied by using P. intermedia ATCC 25611, P. nigrescens ATCC 33563, 174 clinical isolates of P. intermedia sensu lato, and 59 reference strains and 58 clinical isolates of other Prevotella species and/or common oral flora. In addition, the usefulness and reliability of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the differentiation of the two species were examined by comparing the results with those from PCR assays. The controversial lipase test for distinguishing these species was also carried out. Unambiguous differentiation was made by both PCR assays, and the results matched each other. The SDS-PAGE assay was found to misidentify a few strains tested, compared with the results of PCR assays. The lipase test was positive for both species, including the reference strains of P. intermedia and P. nigrescens. We conclude that both PCR assays are simple, rapid, reliable, and specific methods which could be used in clinical studies and that the lipase test is not valuable in the differentiation. The reliable discrimination of the two species by SDS-PAGE is questionable.  (+info)

Mutant and wild type human alpha-synucleins assemble into elongated filaments with distinct morphologies in vitro. (6/1923)

alpha-Synuclein is a soluble presynaptic protein which is pathologically redistributed within intracellular lesions characteristic of several neurodegenerative diseases. Here we demonstrate that wild type and two mutant forms of alpha-synuclein linked to familial Parkinson's disease (Ala30 --> Pro and Ala53 --> Thr) self-aggregate and assemble into 10-19-nm-wide filaments with distinct morphologies under defined in vitro conditions. Immunogold labeling demonstrates that the central region of all these filaments are more robustly labeled than the N-terminal or C-terminal regions, suggesting that the latter regions are buried within the filaments. Since in vitro generated alpha-synuclein filaments resemble the major ultrastructural elements of authentic Lewy bodies that are hallmark lesions of Parkinson's disease, we propose that self-aggregating alpha-synuclein is the major subunit protein of these filamentous lesions.  (+info)

PhoP-PhoQ-regulated loci are required for enhanced bile resistance in Salmonella spp. (7/1923)

As enteric pathogens, Salmonella spp. are resistant to the actions of bile. Salmonella typhimurium and Salmonella typhi strains were examined to better define the bile resistance phenotype. The MICs of bile for wild-type S. typhimurium and S. typhi were 18 and 12%, respectively, and pretreatment of log-phase S. typhimurium with 15% bile dramatically increased bile resistance. Mutant strains of S. typhimurium and S. typhi lacking the virulence regulator PhoP-PhoQ were killed at significantly lower bile concentrations than wild-type strains, while strains with constitutively active PhoP were able to survive prolonged incubation with bile at concentrations of >60%. PhoP-PhoQ was shown to mediate resistance specifically to the bile components deoxycholate and conjugated forms of chenodeoxycholate, and the protective effect was not generalized to other membrane-active agents. Growth of both S. typhimurium and S. typhi in bile and in deoxycholate resulted in the induction or repression of a number of proteins, many of which appeared identical to PhoP-PhoQ-activated or -repressed products. The PhoP-PhoQ regulon was not induced by bile, nor did any of the 21 PhoP-activated or -repressed genes tested play a role in bile resistance. However, of the PhoP-activated or -repressed genes tested, two (prgC and prgH) were transcriptionally repressed by bile in the medium independent of PhoP-PhoQ. These data suggest that salmonellae can sense and respond to bile to increase resistance and that this response likely includes proteins that are members of the PhoP regulon. These bile- and PhoP-PhoQ-regulated products may play an important role in the survival of Salmonella spp. in the intestine or gallbladder.  (+info)

Lipopolysaccharides (LPS) of oral black-pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a way different from that of Salmonella LPS. (8/1923)

Some lipopolysaccharide (LPS) preparations from S- or R-form members of the family Enterobacteriaceae and oral black-pigmented bacteria (Porphyromonas gingivalis and Prevotella intermedia) are known to activate LPS-refractory C3H/HeJ macrophages. When contaminating proteins are removed from R-form LPS of Enterobacteriaceae by repurification, however, this ability is lost. In the present study, we investigated the capacity of LPS from P. gingivalis, P. intermedia, Salmonella minnesota, and Salmonella abortusequi to induce production of tumor necrosis factor (TNF) in gamma interferon-primed C3H/HeJ macrophages before and after repurification. P. abortusequi S-LPS was fractionated by centrifugal partition chromatography into two LPS forms: SL-LPS, having homologous long O-polysaccharide chains, and SS-LPS having short oligosaccharide chains. Prior to repurification, all LPS forms except SL-LPS induced TNF production in both C3H/HeJ and C3H/HeN macrophages. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that repurification removed contaminating protein from the preparations, and repurified SS-LPS and S. minnesota Ra-LPS no longer stimulated TNF production in C3H/HeJ macrophages, although C3H/HeN macrophages remained responsive. In contrast, repurified oral bacterial LPS retained the capacity to induce TNF production in C3H/HeJ macrophages. Oral bacterial LPS preparations also were not antagonized by excess inactive, repurified SL-LPS; Ra-LPS; Rhodobacter sphaeroides lipid A, a competitive LPS antagonist, or paclitaxel, an LPS agonist, and they were comparatively resistant to polymyxin B treatment. Nevertheless, oral bacterial LPS was less toxic to D-galactosamine-treated C3H/HeN mice than was LPS from Salmonella. These findings indicate that the active molecule(s) and mode of action of LPS from P. gingivalis and P. intermedia are quite different from those of LPS from Salmonella.  (+info)

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The effects of sodium lauryl sulphate(SLS) in the presence and absence of antimony(III) on the current efficiency, power consumption and polarization behaviour of zinc were determined. The surface morphologies and deposit crystallographic orientations were also evaluated. The results were compared with glue as the addition agent. The addition of sodium lauryl sulfate to the zinc sulfate solution increased current efficiency, reduced power consumption and improved the surface morphology. Maximum current efficiency and minimum power consumption were achieved on addition of 0.02 mg dm-3 Sb with 1 mg dm-3 sodium lauryl sulfate.. ...
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For example, the anionic detergent sodium dodecyl sulfate (SDS) carries a negatively charged sulfate group on a linear C12 hydrocarbon chain. SDS is considered as a very strong and biologically harsh surfactant. It is able to denature proteins by breaking intra- and intermolecular interactions and thus destroying their biological activity. Other anionic detergents like the bile acid salts Na-cholate and Na-deoxycholate have a rigid steroidal core structure. They do not carry a well defined polar head group opposite to a hydrophobic tail (such as SDS). The polar groups are distributed on different parts of the molecule, resulting in a polar and non-polar side, e.g. Na-deoxycholate carries a carboxylate group at the end of a short hydrocarbon chain and two hydroxyl groups on the steroid structure. The bile acids are less denaturing than the ionic alkyl detergents, possibly due to their rigid steroidal ring structure.. Cationic detergents have a positively charged head group, which is often a ...
Title: Sodium Lauryl Sulfate, a Microbicide Effective Against Enveloped and Nonenveloped Viruses. VOLUME: 3 ISSUE: 1. Author(s): J. Piret, A. Desormeaux and M.G. Bergeron. Affiliation:Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Quebec, Pavillon CHUL,2705 Blvd Laurier, Quebec, QC, Canada G1V 4G2.. Abstract: The number of individuals infected with human immunodeficiency virus (HIV) and other pathogens causing sexually transmitted diseases (STDs) is growing dramatically worldwide. Globally, heterosexual transmission may account for as much as 85-90% of new cases of HIV infection. Latex condoms represent an effective barrier against sexually transmitted pathogens, but unfortunately, their use is not generalized. Therefore, there is an urgent need to develop safe and potent topical microbicides under the control of women to efficiently reduce the spread of sexually transmitted infections. Sodium lauryl sulfate (SLS), an anionic surfactant with protein denaturing potency, ...
Sodium Lauryl Sulfate is a synthetic organic compound that can be used in several types of personal care & cleaning products. Order Sodium Lauryl Sulfate online!
One of the most widely used shampoo ingredients that could damage your skin, is sodium lauryl sulfate. Which shampoos dont contain sodium lauryl sulfate?
SLS NOODLES | SODIUM LAURYL SULFATE Ingredients/Tech Specs:CTFA Name: Sodium Lauryl SulfateINCI Name : Sodium Lauryl SulfateCOUNTRY OF ORIGIN: USA SLS Noodles are a highly active foaming agent that can be used in foaming bath salts, foaming bath bombs, s
Today we will harvest plasmid DNA from E. coli using a purification method called alkaline lysis. In the last lab section, for yeast there needs to be one additional step to help break down the cell wall, but overall goal of each step is the same between both purification protocols--separate the plasmid DNA from the chromosomal DNA and cellular debris, allowing the plasmid DNA to be studied further. The key difference between plasmid DNA and chromosomal DNA is size and this difference is what is used to separate the two components. In this protocol the media is removed from the cells by centrifugation. The cells are first resuspended in a solution that contains Tris, to buffer the cells, and EDTA to bind divalent cations in the lipid bilayer, thereby weakening the cell envelope. Second an alkaline (basic) lysis buffer is added that contains sodium hydroxide and the detergent sodium dodecyl sulfate (SDS). The base denatures the cells DNA, both chromosomal and plasmid, while the detergent ...
Alkaline lysis or alkaline extraction is a method used in molecular biology to isolate plasmid DNA from bacteria. Bacteria containing the plasmid of interest are first cultured, then a sample is centrifuged in order to concentrate cellular material (including DNA) into a pellet at the bottom of the containing vessel. The supernatant is discarded, and the pellet is then re-suspended in an EDTA-containing physiological buffer. The purpose of the EDTA is to chelate divalent metal cations such as Mg2+ and Ca2+, which are required for the function of DNA degrading enzymes (DNAses) and also serve to stabilise the DNA phosphate backbone and cell wall. Glucose in the buffer will maintain the osmotic pressure of the cell in order to prevent the cell from bursting. Tris in the buffer will retain the pH of the cell with 8.0 and RNase will remove the RNA which will disrupt the experiment. Separately, a strong alkaline solution consisting of the detergent sodium dodecyl sulfate (SDS) and a strong base such ...
Sodium Lauryl Sulfate (SLS) is an anionic surfactant naturally derived from coconut and/or palm kernel oil. It usually consists of a mixture of sodium alkyl sulfates, mainly the lauryl. SLS lowers surface tension of aqueous solutions and is used as fat emulsifier, wetting agent, and detergent in cosmetics, pharmaceuticals and toothpastes. It is also used in creams and pastes to properly disperse the ingredients and as research tool in protein biochemistry. SLS also has some microbicidal activity.
Lauryl Sulfate Broth with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli in a laboratory setting. Lauryl Sulfate Broth with MUG is not intended for use in the diagnosis of disease or other conditions in humans.. The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.. Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby. During their investigation, Sodium Lauryl Sulfate produced the best results for inhibition of organisms other than coliforms. Feng and Hartman developed a rapid assay for E. coli by incorporating ...
Lauryl Sulfate Broth with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli in a laboratory setting. Lauryl Sulfate Broth with MUG is not intended for use in the diagnosis of disease or other conditions in humans.. The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.. Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby. During their investigation, Sodium Lauryl Sulfate produced the best results for inhibition of organisms other than coliforms. Feng and Hartman developed a rapid assay for E. coli by incorporating ...
The influence of the surfactants of sodium lauryl sulfate (SLS) and Tween 80 on carbamazepine-nicotinamide (CBZ-NIC) cocrystal solubility and dissolution behaviour has been studied in this work. The solubility of the CBZ-NIC cocrystal was determined by measuring the eutectic concentrations of the drug and the coformer. Evolution of the intrinsic dissolution rate (IDR) of the CBZ-NIC cocrystal was monitored by the UV imaging dissolution system during dissolution. Experimental results indicated that SLS and Tween 80 had little influence upon the solubility of the CBZ-NIC cocrystal but they had totally opposite effects on the IDR of the CBZ-NIC cocrystal during dissolution. SLS significantly increased the IDR of the CBZ-NIC cocrystal while Tween 80 decreased its IDR.
Sodium Lauryl Sulfate is useful in a wide variety of personal care applications in which viscosity building and foam characteristics are of importance. Because of its low salt content, this product is particularly useful in formulations that are sensitive to high levels of sodium chloride. It is compatible with alkanolamides and amphoterics so that maximum optimization of foam and viscosity characteristics can be reached in the finished product. It can be incorporated into shampoos, handsoaps, bath products, shaving creams and medicated ointments. It is especially useful for opaque, pearlescent, or cream products.
If you looked at the ingredient list on your body wash, shampoo, toothpaste and cleaning products, youd probably find most contain sodium lauryl sulfate (SLS). It is a known irritant to human skin.
The adsorption of a biologically important glycoprotein, mucin, and mucin-chitosan complex layer formation on negatively charged surfaces, silica and mica, have been investigated employing ellipsometry, the interferometric surface apparatus, and atomic force microscopy techniques. Particular attention has been paid to the effect of an anionic surfactant sodium, dodecyl sulfate (SDS), with respect to the stability of the adsorption layers. It has been shown that mucin adsorbs on negatively charged surfaces to form highly hydrated layers. Such mucin layers readily associate with surfactants and are easily removed from the surfaces by rinsing with solutions of SDS at concentrations ≥0.2 cmc (1 cmc SDS in 30 mM NaCl is equal to 3.3 mM). The mucin adsorption layer is negatively charged, and we show how a positively charged polyelectrolyte, chitosan, associates with the preadsorbed mucin to form mucin-chitosan complexes that resist desorption by SDS even at SDS concentrations as high as 1 cmc. Thus, ...
Re: Sodium Lauryl Sulfate in medicines? Thanks, guys johnep - the opemprazole is a timed release med, so I cant see how aiding dissolution would be an asset. Jeff - Im not registered with any particular pharmacy, I get my script, and fill it where ever is convenient. I did ask at the original pharmacy if the brand could be changed, and was ...
Sodium lauryl sulfate has been linked to a significant increase in number of canker sores In The Mouth when compared to brushing with SLS-free toothpaste. Verve was created for people suffering from frequent cankers and mouth sores.
lauryl sulfate & lauryl sulfate online Wholesalers - choose lauryl sulfate from 3462 list of China lauryl sulfate Manufacturers of page 9.
Cetearyl alcohol and sodium lauryl sulphate make an emulsifying wax. We use this wax in our conditioners and hair moisturisers. In our quantitative ingredients list, if ingredients are listed with an ampersand, it means they are combined together to make one material.
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what is sds? sodium dodecyl benzene sulfonate. Just curious. Scott Coutts ,scott.coutts at med.monash.edu.au, wrote in message news:3F024A95.40702 at med.monash.edu.au... , Hi! , , Does anyone know of a way that I can assay for SDS concentration in a , sample containing protein? I want to know how much detergent remains , after attempts at removal. I need to assay for regular (non-labelled) , SDS, by the way. , , Also, Im thinking of removing the SDS from some protein samples using a , resin, perhaps Dowex 1-X2 or 1-X8 or similiar. Im thinking of doing it , in a batch method and then spinning the resin out through a frit. Does , anybody have any comments about this? I.e. are there better resins for , this type of application? Better ways of going about it? Is this likely , to be more efficient than acetone or TCA precipitation (I dont care , about protein function or recovery of secondary/tertiary structure). , , Lastly, does anyone have any experience with Pierces SDS-Out , ...
Vegetable based, melt and pour soap base, free from the surfactants Sodium Lauryl Sulfate and Sodium Laureth Sulfate. Product Characteristics: Contains no Sodium Lauryl Sulfate Contains no Sodium Laureth Sulfate Vegetable b.
Hellow I wanted to thank all those who responded--privatly and on this beautiful newsgroup--to my help request. In addition, I used the BCA method from Pierce successfully. I have to make BSA standards in the same buffer solution and do 5 folds dilution. That is taking the SDS concentration to 0.4% in all sample and standards. I added the other components of the SDS gel loading buffer (DTT and BrPhenol Blue) after Im satisfied with the measurments. ----- From my small experience, direct UV measurment is not reliable, the presence of 1% SDS does not allow direct total protein measurment using Peirce BCAs nor Biorads kits. Mohammed BaSalamah ...
Poll: Time and voltage for wet transfer to PVDF - posted in SDS-PAGE and Western Blotting: Im trying to use tris/glycine/20%MeOH (Towbins) with 0.1% SDS to wet transfer onto PVDF. I think Im blowing right through the membrane; my markers are not on the membrane or the gel after 100V for 2hrs. If you use Towbins buffer and a wet transfer system to PVDF (mine is bioRad mini): What is your: Voltage: Time: SDS concentration:
The assay was calibrated using SDS to be able to compare it to existing assays. Our measurements are shown in the graph on right. It shows that the assay is very sensible at low SDS concentrations. Next, the influence of the culture media was determined. We used LB medium for the initial growth of the cells, and induced the production of AlnA in M9 medium. The background influence of the media on the assay were measured, as shown below. It shows that LB medium already has some emulsification effect, but M9 shows very little influence. ...
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Dec 23, 2011 . Sodium lauryl sulfate (SLS) is an anionic detergent that has been used as the major or sole surfactant in most dentifrices. But it is known to local. ...
Beyond providing Skin Deep® as an educational tool for consumers, EWG offers its EWG VERIFIED™ mark as a quick and easily identifiable way of conveying personal care products that meet EWGs strict health criteria. Before a company can use EWG VERIFIEDTM on such products, the company must show that it fully discloses the products ingredients on their labels or packaging, they do not contain EWG ingredients of concern, and are made with good manufacturing practices, among other criteria. Note that EWG receives licensing fees from all EWG VERIFIED member companies that help to support the important work we do. Learn more , Legal Disclaimer ...
You can spot most offending ingredients in makeup removers by reading the list of ingredients. The National Eczema Association can guide you on which ingredients to avoid, too.. However, if your makeup remover does contain an ingredient that may irritate your face, you can try making use of wetness and dryness effects. Your skin is more absorbent when its wet and a better barrier when its dry, so dont apply irritating removers to wet skin. The side effects will be worse than when your skin is dry [source: Tofte].. What makeup remover is probably in your kitchen right now? Find out next.. ...
All rights reserved. The published material is being distributed without warranty of any kind, either expressed or implied. Neither ILO nor WHO nor the European Commission shall be responsible for the interpretation and use of the information contained in this material ...
She is a beautiful soul. She is not only my editor, she has become my friend. I admire her from afar for the person I could never be. Eleven years writing an emotional story of love, loss, and death tethers through my veins. She taught me a gentle way to transition words, or shall I say Bridge to Story. Her name is E.J. Runyon and she has made a difference in my life.. ...
Finally, the patient realized that he had accidentally been using a toothpaste containing sodium lauryl sulfate for a few weeks prior to symptom onset. He has not experienced any recurrences upon switching back to his regular toothpaste without sodium lauryl sulfate. ...
Jul 23, 2013But if you have sensitive teeth, you might want to consider something that doesnt contain sodium lauryl sulfate. I couldnt find any toothpaste that had fluoride, baking soda, and was SLS-free; but if you choose a toothpaste that doesnt have baking soda and still want its benefits, you can consult your dentist on the best way to do that.. Get Price ...
L. Shi, X. Jing, H. Gao, Y. Gu and L. Zheng,Ionic liquid-induced changes in the properties of aqueous sodium dodecyl sulfate solution: effect of acidic/basic functional groups,Colloid Polym. Sci.,2013...
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Name:Sodium dodecyl sulfate,CAS:151-21-3.Properties:Molecular Fomula:C12H25NaSO4,Molar Mass:288.378,Melting Point:206℃,Solubility:ca. 150 g/L (20℃),MSDS,Hazard,Safety.
Weve done conditioner only washing, clay washing, honey washing, baking soda cleansing, ACV (apple cider vinegar) cleansing and various other ways to clean hair without the use of harsh chemicals like sodium sodium lauryl sulfate (SLS) and ammonium lauryl sulfate (ALS). Now, we have water only washing. This trend has ...
Sulfates have been steadily gaining a bad rep in the beauty industry for the last few years. Why and what the heck are they? Sodium lauryl sulfate, ammonium lauryl sulfate and others are found in basically any cleaning product that lathers and foams: soaps, shampoos, bubble bath, toothpaste, and yes, oven cleaner. Heres whats known about sulfates: 1) They can irritate the scalp, skin, and eyes. 2)They strip the essential oils from hair, which is what gives you that squeaky clean feeling. But they also cause the hair to dry out and can lead to breaking and damage. 3) They can cause allergic reactions. 4) Theyre bad for the environment (they can kill fresh water fish) and dont break down easily. 5) The jury is still out on whether theyre carcinogens. Recent studies have shown that sulfates are safe to be applied or consumed, but other studies have shown that they turn into a carcinogen when combined with other chemicals. (Some of which, yes, are also found in shampoos). The FDA isnt all
Sodium Lauryl Sulfoacetate (SLSA) is a mild coconut and palm oil derived surfactant. SLSA does not cause the skin irritation that sulfates may, making it an excellent choice for facial cleansers ...
Sodium Lauryl Sulfoacetate -SLSA on sale Description: is a great surfactant and foaming agent. A powder that is used to make bath salts, bath fizzies/ bombs, bubble bars, milk bath bubble and foam. Works well in soft and hard water. This is a very airborne product, you will need a mask and a contained space when maki
Therefore the constant exposure to Sodium Lauryl Sulfate (SLS) in the combination of detergents and hygiene is said to be problematic for long term health. SLS Free Detergents. The good news is, there is now a large number of green detergents available for sale that do not have this ingredient in it. These are known as SLS free detergents. Many .... Get Price ...
Also called SLSA, it is a foaming surfactant used in cleansing formulas. It is said to be milder for skin than Sodium Laureth Sulfate (SLES).. ...
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Looking for online definition of sodium lauryl sulfate in the Medical Dictionary? sodium lauryl sulfate explanation free. What is sodium lauryl sulfate? Meaning of sodium lauryl sulfate medical term. What does sodium lauryl sulfate mean?
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1G8C: Structure of the bovine antimicrobial peptide indolicidin bound to dodecylphosphocholine and sodium dodecyl sulfate micelles.
The single-banding components of bovine transferrin from animals homozygous for the four transferrin variants found in the U.K. were isolated. Sedimentation equilibrium ultracentrifugation and sodium dodecyl sulphate-polyacrylamide-gel electrophoresis showed that the bands of a single variant have molecular weights of 77500 and 73300 respectively. The different bands of a single variant and single bands of different variants show no evidence of size heterogeneity or of low-molecular-weight peptides being split off after reduction in 6m-guanidine hydrochloride. The two slower bands of a single variant, which both contain 2 molecules of sialic acid/molecule of protein, have the same molecular weight and amino acid composition, and give identical peptide maps, although differences in composition and peptide maps occur between the different variants. The results support the concept that bovine transferrin is essentially a single polypeptide chain, but they do not explain differences in ...
Contents of the 15 Chapter for This Anionic Surfactants Market Study:-. Chapter 1: to describe Global Anionic Surfactants Market Introduction, product scope, market overview, market opportunities, market risk, market driving force;. Chapter 2: to analyze the top manufacturers of Global Anionic Surfactants Market, with sales, revenue, and price of Global Anionic Surfactants Market, in 2016 and 2017;. Chapter 3: to display the competitive situation among the top manufacturers, with sales, revenue and market share in 2016 and 2017;. Chapter 4: to show the Global Anionic Surfactants market by regions, with sales, revenue and market share of Global Anionic Surfactants Market, for each region, from 2012 to 2017;. Chapter 5, 6, 7, 8 and 9: to analyze the key regions, with sales, revenue and market share by key countries in these regions;. Chapter 10 and 11: to show the market by type and application, with sales market share and growth rate by type, application, Methodology, Analyst Introduction, Data ...
Jan 23, 2016· Top 5 SLS Free Toothpaste Brands. January 23, 2016 Blog. Are you looking for a natural SLS free toothpaste? . If you are really trying to use the best natural toothpaste for your teeth and health overall, then you need to grab sodium lauryl sulfate free toothpaste. You just need to use SLS free toothpaste and of course without all other ...
The removal of crystal violet from aqueous solution by NiFe2O4 magnetic nanoparticles treated with sodium dodecyl sulfate was investigated. The modified magnetic nanoparticles were prepared by chemical reaction of a mixture of Ni+2 and Fe+3 ions mixture in aqueous solution at the presence of ammonia and then sodium dodecyl sulfate was utilized as an ionic surfactant to modified the obtained magnetic nanoparticles. The morphologies of synthesized magnetic nanoparticles was characterized by electron microscopy techniques. The X-ray diffraction results confirmed the formation of magnetic nanoparticles with face centred cubic structure and the crystallite size was found to be 19 nm. The Fourier transform infrared spectrums confirmed the coating of sodium dodecyl sulfate on synthesized magnetic nanoparticles. The effects of different parameters, such as pH, initial concentration of dye and contact time were studied on the adsorption of crystal violet onto modified magnetic nanoparticles. The equilibrium data
Define anionic detergent: any of a class of synthetic detergents usually consisting essentially of an alkali metal salt or an ammonium salt of a…
A series of porous block copolymer (BCP) particles with controllable morphology and pore sizes was fabricated by tuning the interfacial behavior of BCP droplets in oil-in-water emulsions. A synergistic adsorption of polystyrene-b-poly(4-vinylpyridine) (PS-b-P4VP) BCPs and sodium dodecyl sulfate (SDS) to the surface of the emulsion droplet induced a dramatic decrease in the interfacial tension and generated interfacial instability at the particle surface. In particular, the SDS concentration and the P4VP volume fraction of PS-b-P4VP were key parameters in determining the degree of interfacial instability, leading to different types of particles including micelles, capsules, closed-porosity particles, and open-porosity particles with tunable pore sizes ranging from 10 to 500 nm. The particles with open-porosity could be used as pH- responsive, high capacity delivery systems where the uptake and release of multiple dyes could be achieved.. ...
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sodium lauryl ether sulfate sles 70%, CAS No. 68585-34-2 Specification sodium lauryl ether sulfate SLES CAS NO. 68585-34-2 C12H26Na2O5S best price Product Name Sodium lauryl ether sulfate Synonyms SODIUM LAURYL ETHER SULFATE;NAXOLATE...
Sodium Lauryl Sulfate and Sodium Laureth Sulfate. ... Sodium Lauryl Sulfate and Sodium Laureth ... 1983 and re-reviewed in 2002 by the Cosmetic Ingredient ...
sodium lauryl ether sulphate to sell - 185 sodium lauryl ether sulphate online Wholesalers provide popular sodium lauryl ether sulphate from China.
Sodium Lauryl Sulfate; Sulfuric Acid, Monododecyl Ester, Sodium Salt; Dodecyl Sulfate, sodium salt; SLS; SDS; Neutrazyme; Lauryl Sulfate Sodium Salt; SDS Running Buffer; Sodium lauryl sulfate; Sulfuric acid monododecyl ester sodium salt ...
1NG8: The Structure, Cation Binding, Transport and Conductance of Gly15-Gramicidin a Incorporated Into Sds Micelles and Pc/Pg Vesicles
Iron phosphate was prepared from iron nitrate and phosphoric acid with sodium dodecyl sulfate at various stirring hours. The chemical composition of the obtained samples was estimated from ICP and XRD measurements. Particle shape and size distribution were observed by SEM images and laser diffraction/scattering methods. Further, the catalytic activity was studied with the decomposition of the complex between formaldehyde, ammonium acetate, and acetylacetone. The peaks of FePO|sub|4|/sub| were observed in XRD patterns of samples prepared in Fe/Ce = 10/0 and then heated at 600°C. Other samples were amorphous in XRD patterns. Iron-cerium phosphates had high catalytic activity for the decomposition of the complex.
Sodium Lauryl Ether Sulfate (SLES 70%)(id:5790279). View product details of Sodium Lauryl Ether Sulfate (SLES 70%) from Jiaxing Zanyu Technology Development Co.,Ltd. manufacturer in EC21
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Sodium Dodecyl Sulfate, or SDS, is an anionic detergent with widespread use in industrial and household cleaning products, scientific laboratories, and personal care products such as toothpaste and shampoo. The potential toxicity of SDS has been well-characterized in whole organism studies and its potential effects on the environment continue to be studied. Herein, we undertake a chemical-genetic screen to explore whether low concentrations of SDS have any discernible effects at the cellular level. Our screen of the homozygous diploid yeast deletion collection identified numerous gene deletions that confer sensitivity to SDS. Subsequent bioinformatic and biological analyses reveal that yeast unable to synthesize tryptophan are especially sensitive to the presence of SDS. Interestingly, even wild-type yeast with an intact tryptophan biosynthetic pathway exhibit growth defects in the presence of SDS on media lacking tryptophan. Altogether, we have shown that low levels of SDS, primarily through ...
CIR) Expert Panel and found to be safe for use in cosmetic and personal care products. SLS and SLES can cause skin irritation in some persons, which is one reason why it is important to follow the label instructions when using a cosmetic product. Complete reports on both ingredients are available from CIR. Substances known to be carcinogenic have been classified and registered by several international organizations, such as the World Health Organization or the International Agency for the Research of Cancer as well as the US Environment Protection Agency and the European Union. None of these organizations have classified SLES and SLS as carcinogens. There is no direct or circumstantial evidence that these two ingredients have any carcinogenic potential. The studies that have been conducted on SLS and SLES indicate that both are safe under proper conditions of use.. ...
Revita, the most efficient hair growth stimulating shampoo available in the market is the final result of DS Laboratories efforts on cutting edge research. Revita is a powerful and unique SLS/SLES free combination of active ingredients specially designed to maintain scalp vitality and act on folicle dysfunctions in order to achieve best results in short periods of time. Sodium Lauryl Sulfate and Sodium Laureth Sulfate, commonly used low cost detergents in shampoos and cleansers, are linked to skin irritation, skin drying and hair loss due to follicle attack. Revita is Sodium Lauryl Sulfate and Sodium Laureth Sulfate free, providing a high quality scalp skin safe shampoo product. Revita was developed with a cost-no-object approach. Revitas compounds have been chosen based exclusively on their properties, quality and efficacy (in the opposite of the majority of available products, which are usually developed with production costs in mind). The final result is a very high quality shampoo product ...
Sodium Lauryl Sulfate (SLS).. This chemical is added to toothpaste to give you a mouthful of bubbles. Foam is good for marketing because we associate foam with clean.. If your mouth foams it must be clean - right?. If you Google Sodium Lauryl Sulfate youll find out its an industrial chemical that is used by scientists in laboratory experiments. Its frequently employed as a skin irritant in tests.. SLSs foamy cleaning properties are very useful in industry too. The same SLS makes the foam that cleans the bugs and road grime from your car in your local carwash.. Imagine heavy machinery in a factory. These machines need a lot of grease and oil to reduce friction so they dont seize up while operating. That grease and oil slops all over the place - so at the end of the day they use SLS to de-grease the machinery.Thats the same chemical thats foaming up your toothpaste.. Fluorides. Fluorides are a complex group of chemicals you could write a book about.. In the USA and other countries with ...
Buy Adiva Online! Adiva is available as capsules for oral administration containing either 50 mg or 200 mg of Adiva and the following inactive ingredients: lactose monohydrate, magnesium stearate, sodium lauryl sulfate, and sodium starch glycolate. The capsule shell contains the following inactive ingredients and dyes: gelatin, sodium lauryl sulfate, titanium dioxide, and/or yellow iron oxide.
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A further investigation of a chromatographic system allowing determination of hydrogen ions is reported. For this purpose an octadecylsilica column dynamically modified with sodium dodecylsulfate (SDS) or lithium dodecylsulfate (LDS) was used as stationary phase and a slightly acidified electrolyte (usually KCl)-SDS solution was used as the eluent. The concentration of SDS, KCl and the acidity of the eluent affected the structure of aggregates formed by the molecules of dodecylsulfate at the surface of the stationary phase. These aggregates of dodecylsulfate were found to be responsible for the appearance of a chromatographic peak attributed to the presence of H3O+ ions in a sample. Other cations in the sample could be separated in the same manner, permitting the simultaneous separation of monovalent cations from H3O+. The detection limit for H3O+ ions was 2.25·10−6 M using an eluent comprising 0.3 mM LDS, 50.0 mM KCl and 0.10 mM H2SO4. The proposed method is shown to be applicable for the ...
Definition of sodium dodecyl sulfate. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
Sodium Lauryl Ether Sulfate (SLES) Market will likely surpass USD 1.5 billion by 2024 as demand for household detergents and cleaners increases, says latest study by Global Market Insights, Inc.
lau·ryl alcohol .lȯr əl , .lär n a compound C12H26O also a liquid mixture of this and other alcohols used esp. in making detergents (as sodium lauryl sulfate)
Page contains details about SDS micelles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. Applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain. As a result, the distance of migration through the gel in SDS-PAGE electrophoresis can be assumed to be directly related only to the size of the protein. More than 99% pure. One tablet weighs 0.5 g and can be dissolved in deionized water to the desired volume.
Special toothpaste designed for all of us canker sore sufferers. The sad thing is that what makes it so special isnt whats in it but whats left out - SLS to be exact. SLS (or sodium lauryl sulfate) is what causes the foaming reaction you get with toothpastes, it also causes small microscopic tears in your mouth (which considering what I use it for in the lab it makes a lot of sense ...
In cosmetics, this large group of ingredients is used mainly as a cleansing agent; they include sodium lauryl sulfate and sodium laureth sulfate.
EarthTurns.com sells chemical free personal care products. We select green personal care products that do not contain harmful chemicals like sodium lauryl sulfate, detergents or parabens.
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Randomised and controlled. 3 areas were evaluated: area to which deionised water was applied (negative control), area to which Bio-Oil was applied, and area to which Sodium Lauryl Sulfate 1% solution was applied (positive control - a known skin irritant). Test products applied via a patch to the volar forearm and removed after 24 hours. Skin evaluated and reactions noted at 24, 48, 72 and 96 hours after application. Dermatologist present during the evaluations. Skin reactions rated on a scale from 0 - 4 (with 0 being no response and 4 being fiery redness ...
Randomised and controlled. 3 areas were evaluated: area to which deionised water was applied (negative control), area to which Bio-Oil was applied, and area to which Sodium Lauryl Sulfate 1% solution was applied (positive control - a known skin irritant). Test products applied via a patch to the volar forearm and removed after 24 hours. Skin evaluated and reactions noted at 24, 48, 72 and 96 hours after application. Dermatologist present during the evaluations. Skin reactions rated on a scale from 0 - 4 (with 0 being no response and 4 being fiery redness ...
SDS (sodium dodecyl sulfate) lysis buffer[edit]. SDS is ionic denaturing detergent. Hot SDS buffer is often used when the ... Ionic detergents like sodium dodecyl sulfate (SDS) and cationic detergents like ethyl trimethyl ammonium bromide are denaturing ... 50 mM sodium fluoride (NaF). *0.2 mM fresh sodium orthovanadate (Na3VO4.2H2O, it has phosphatase inhibitor function because it ...
The combined use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel allows to ... In addition, SDS (sodium dodecyl sulfate) is used. About 1.4 grams of SDS bind to a gram of protein, corresponding to one SDS ... SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis), is a discontinuous electrophoretic system developed by ... Manning M, Colón W (2004). "Structural basis of protein kinetic stability: resistance to sodium dodecyl sulfate suggests a ...
Ochs D (1983). "Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels". Anal Biochem. 135 (2): 470-4. doi:10.1016/ ... 1988). "Improvement and simplification of low-background silver staining of proteins by using sodium dithionite". ...
Sodium dodecyl sulfate (SDS) (C 12H 25NaO 4S; mW: 288.38) (only used in denaturing protein gels) is a strong detergent agent ... For proteins, sodium dodecyl sulfate (SDS) is an anionic detergent applied to protein samples to coat proteins in order to ... Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the ... Quandt N, Stindl A, Keller U (1993). "Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis for Mr Estimations of High- ...
Treatment with sodium dodecyl sulfate triggers dissociation of the subunits. The cytoplasmic tail of MUC1 is 72 amino acids ...
Dandruff shampoos often contain sodium dodecyl sulfate, a noted skin irritant. Steroid and tar preparations have also been used ...
Examples of anionic surfactants include sodium dodecyl sulfate and dioctyl sodium sulfosuccinate. Included in this class of ... surfactants are sodium alkylcarboxylates (soaps). Cationic surfactats are similar in nature to anionic surfactants, except the ...
For example, the self-assembly of sodium dodecyl sulfate (SDS) in DES has recently been studied, implying DES can form ... Pal, M; Rai, R.; Yadav, A.; Khanna, R.; Baker, GA.; Siddharth, P. (2014). "Self-Aggregation of Sodium Dodecyl Sulfate within ( ... in the first he pointed out that activity coefficients in DES do appear to deviate wildly away from their values in sodium ...
Sodium dodecyl sulfate (SDS) is the most commonly used surfactant in MEKC applications. The anionic character of the sulfate ...
Surfactants such as sodium dodecyl sulfate, Triton and nanoparticles have been used successfully. During the electrospinning ... "Electroblowing and electrospinning of fibrous diclofenac sodium-cyclodextrin complex-based reconstitution injection". Journal ...
Sodium dodecyl sulfate (SDS) (C12H25NaO4S; mW: 288.38) (only used in denaturing protein gels) is a strong detergent agent used ... For proteins, sodium dodecyl sulfate (SDS) is an anionic detergent applied to protein samples to coat proteins in order to ... Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the ... Schägger H, von Jagow G (Nov 1987). "Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of ...
Phase Behavior in a Water/Phytoglycogen/Sodium Dodecyl Sulfate (SDS) System". Bioscience, Biotechnology, and Biochemistry. 61 ( ...
measured the partitioning of 14 radiolabeled amino acids using sodium dodecyl sulfate (SDS) micelles. Also, amino acid side ...
"Spectral studies of toluidine blue O in the presence of sodium dodecyl sulfate" (PDF). Digest Journal of Nanomaterials and ... When these hard cations encounter the hard anions e.g. in form of sulfate they form salts without regard for the precise ... At pH 1.0 it stains only sulfated polysaccharides and at pH 2.5 also stains carboxyl group containing sugars such as sialic ... Commercially available batches usually contained about 49% of the actual dye and rest used to be Sulfate, boric acid, dextrin ...
sodium dodecyl sulfate. Disulfide bond reducersEdit. Agents that break disulfide bonds by reduction include:[citation needed] ... sodium salicylate, dimethyl sulfoxide (DMSO), propylene glycol, and urea.[22] These chemical denaturing agents lower the ...
SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by gel staining. ...
"Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis" (PDF). J. Biol. Chem. 252 ...
Sodium dodecyl sulfate (SDS) interacts with proteins, denaturing them, and giving them a negative charge. Since all proteins ...
Martínez TF, Alarcón FJ, Díaz-López M, Moyano FJ (2000). "Improved detection of amylase activity by sodium dodecyl sulfate- ...
Molecular Dynamics Simulations of Sodium Dodecyl Sulfate Micelles in Water: The Effect of the Force Field. The Journal of ... Tang, Xueming; Koenig, Peter H.; Larson, Ronald G. (2014-04-10). "Molecular Dynamics Simulations of Sodium Dodecyl Sulfate ...
AE1 was discovered following SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) of erythrocyte cell membrane ...
"Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel ...
An aqueous solution containing 50% v/v isopropyl alcohol and 1% w/v sodium dodecyl sulfate (found in most household detergents ... Isopropyl alcohol dissolves the outer waxy covering, while the sodium dodecyl sulfate attacks the mealybugs themselves. ...
The dye also forms a complex with the anionic detergent sodium dodecylsulfate (SDS). The formation of this complex stabilizes ... and ammonium sulfate (or aluminium sulfate). The Bradford assay uses the spectral properties of Coomassie Brilliant Blue G-250 ...
Sodium dodecyl sulfate. Dobavljeno iz "https://sh.wikipedia.org/w/index.php?title=Natrijum_dodecil_sulfat&oldid=33644085" ... Chahine L, Sempson N, Wagoner C: The effect of sodium lauryl sulfate on recurrent aphthous ulcers: a clinical study. Compend ... Marrakchi S, Maibach HI: Sodium lauryl sulfate-induced irritation in the human face: regional and age-related differences. Skin ... Agner T: Susceptibility of atopic dermatitis patients to irritant dermatitis caused by sodium lauryl sulphate. Acta Derm ...
Some typical members of this group are: sodium dodecyl sulfate (anionic), benzalkonium chloride (cationic), cocamidopropyl ... Amphoterism Bubbles in abiogenesis Emulsion Free surface energy Surfactant Lipid polymorphism Sodium dodecyl sulfate Wetting ... Examples, with the lipophilic part of the molecule represented by an R, are: carboxylates: RCO2−; sulfates: RSO4−; sulfonates: ...
Properties of Secondary Butanol and Tertiary Butanol in Water and Aqueous Micellar Systems of Sodium Dodecyl Sulphate - Section ... Properties of Secondary Butanol and Tertiary Butanol in Water and Aqueous Micellar Systems of Sodium Dodecyl Sulphate". Journal ...
Since the technique involves the use of SDS-PAGE which utilizes the effect that sodium-dodecyl sulfate has on proteins which is ... First, proteins are prepared with a mixture that exposes them to the denaturing sodium dodecyl sulfate (SDS) agent. This ...
Two parameters were used: isoelectric focusing in the first dimension sodium dodecyl sulfate electrophoresis in the second ...
It contains 1% sodium dodecyl sulfate (SDS) (w/v) to puncture holes in cellular membranes, and 200mM NaOH. It is used in ...
... of three forms of rabbit microsomal cytochrome P-450 by peptide mapping utilizing limited proteolysis in sodium dodecyl sulfate ...
Burnette, W. Neal (April 1981). "Western Blotting: Electrophoretic Transfer of Proteins from Sodium Dodecyl Sulfate- ... If alkaline transfer methods are used, the DNA gel is placed into an alkaline solution (typically containing sodium hydroxide) ...
Potassium sodium tartrate - yemek asidi. *Potassium sorbate - koruyucu. *Potassium sulfate - mineral tuz, seasoning ... Sodium aluminium phosphate - asitlik düzenleyici, emülgatör. *Sodium aluminosilicate (sodium aluminium silicate) - topaklanma ... Kalsiyum sulfate - flour treatment agent, mineral salt, sequestrant, improving agent, firming agent ... Sodium alginate - kıvam arttırıcı, bitkisel sakız, stabilizer, jelleştirici, emülgatör. * ...
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques to separate ... Wiltfang J, Arold N, Neuhoff V (1991). "A new multiphasic buffer system for sodium dodecyl sulfate-polyacrylamide gel ... Schägger H, von Jagow G (1987). "Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of ... sodium bisulfite) that move into the gel ahead of the proteins to maintain a reducing environment. An additional benefit of ...
Examples include: sodium lauryl sulfate (also known as sulfuric acid mono dodecyl ester sodium salt) and related potassium and ... A common example is sodium lauryl sulfate, with the formula CH3(CH2)11OSO3Na. Also common in consumer products are the sulfate ... Natural sulfate esters[edit]. The reduction of sulfate in nature involves the formation of one or sometimes two sulfate esters ... C4 or C5 sulfate and an additional metabolite. The highest irritant of the alkyl sulfates is sodium laurylsulfate, with the ...
... sodium dodecyl sulfate, SLS, or SDS), and the related alkyl-ether sulfates sodium laureth sulfate (sodium lauryl ether sulfate ... Prominent alkyl sulfates include ammonium lauryl sulfate, sodium lauryl sulfate ( ... Popular surfactants in the biochemistry laboratory are sodium lauryl sulfate (SDS) and cetyl trimethylammonium bromide (CTAB). ... Anionic: sulfate, sulfonate, and phosphate, carboxylate derivatives[edit]. Anionic surfactants contain anionic functional ...
To separate proteins by size only, protein mixtures can be coated with a chemical called sodium dodecyl sulfate (SDS) to give ...
NH3 and the surfactant sodium dodecyl sulfate followed by heating and stirring for 24 hours at 80 °C: 2 LaCl3+ 3 H2O + 3 NH3 → ...
Sodium dodecyl sulfate (SDS), a common detergent, may be found in protein extracts because it is used to lyse cells by ... the Bradford protein assay is less susceptible to interference by various chemical compounds such as sodium, potassium or even ...
The application of sodium dodecyl sulphate-polyacrylamide gel electrophoresis to the taxonomic identification of the total body ...
... sodium dodecyl sulfate, thiourea, and urea.[citation needed] ... Antichaotropic salts such as ammonium sulphate can be used to ...
Early studies used the sodium salt, but it was soon replaced with the potassium sodium salt to prevent hypokalaemia, which is ... On the other hand, the use of milder conditions such as acetyl sulfate leads to incomplete sulfonation. Recently, the atom ... "Polymerization of Styrene Sulfonate Ethyl Ester and Styrene Sulfonate Dodecyl Ester by ATRP: Synthesis and Characterization of ... In this way, various salts of the polymer can be prepared, leading to sodium, calcium, and other salts: (CH2CHC6H4SO3H)n + n ...
Sodium dodecyl sulfate polyacrylamide gel electroforesis) - naatriumdodetsüülsulfaat-polüakrüülamiid geelelektroforees. SDS ...
... sodium dodecyl sulfate (SDS),[19][21] and/or dodecanethiol.[22] Once the particles have been formed in solution they must be ... Reduction via sodium borohydride[edit]. The synthesis of silver nanoparticles by sodium borohydride (NaBH4) reduction occurs by ... The silver seeds are synthesized by mixing silver nitrate and sodium citrate in aqueous solution and then rapidly adding sodium ... There are many different wet synthesis methods, including the use of reducing sugars, citrate reduction, reduction via sodium ...
... published a new method for DNA isolation and purification from nuclei employing sodium dodecyl sulfate that became widely used. ... Kay, E. R. M.; Simmons, N. S.; Dounce, A. L. (1952). "An improved preparation of sodium desoxyribonucleate". Journal of the ... "This Week's Citation Classic - An improved preparation of sodium desoxyribonucleate" (PDF, 0.1 MB). Current Contents. 24: 10. ...
... anti-caking agent Aluminium sodium sulfate - mineral salt Aluminium sulfate - mineral salt Amaranth - color (red) (FDA: [ ... antioxidant Dodecyl gallate - antioxidant Echinacea - EDTA - Antioxidant, Chelating Agent Egg - Egg yolk - Egg white - ... acidity regulator Sodium hydroxide - mineral salt Sodium lactate - food acid Sodium malates - food acid Sodium metabisulfite - ... anti-caking agent Sodium formate - preservative Sodium fumarate - food acid Sodium gluconate - stabiliser Sodium hydrogen ...
... sodium dodecyl sulfate and Savvy (1.0% C31G), act by disrupting the viral envelope, capsid or lipid membrane of microorganisms ... Cellulose sulfate microbicide trial stopped. "Research: Cellulose sulfate microbicide trial stopped". Retrieved 1 April 2018., ... Carrageenans are a family of linear sulfated polysaccharides chemically related to heparan sulfate, which many microbes utilize ... World Health Organization Cellulose sulfate microbicide trial stopped. "Research: Cellulose sulfate microbicide trial stopped ...
In non-denaturing gels this enzyme protein exhibited a molecular mass of 160 kDa, while in sodium dodecyl sulfate- ... resulted after sodium dodecyl sulfate gel electrophoresis27. AT exhibited an apparent Km for trehalose of about 4.7 mM at pH ...
Sodium Dodecyl Sulfate) gel electrophoresis until 120 °C. Schink, Bernhard; Pfennig, Norbert (1982). "Propionigenium Modestum ... "The sodium ion translocating adenosine triphosphatase of Propionigenium modestum pumps protons at low sodium ion concentrations ... Dimroth, P.; Wang, H.; Grabe, M.; Oster, G. (1999). "Energy transduction in the sodium F-ATPase of Propionigenium modestum". ... but the F1F0 ATPase of Propionigenium modestum is the first discovered which uses sodium ions (Na+). The discovery of the ...
... sodium dodecyl sulfate (SDS) or Guanidinium chloride (3 M), Guanidinium thiocyanate (1 M) and urea (4 M)[disputed (for: no ...
... which showed that proteins could be dissolved in sodium dodecyl sulfate (SDS), reliably separated by polyacrylamide gel ... The title of the paper was "The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel ... "The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis". J. Biol. Chem. 244 ( ...
... or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple ...
8 mol/l Guanidinium chloride 6 mol/l Lithium perchlorate 4.5 mol/l Sodium dodecyl sulfate Agents that break disulfide bonds by ... They include: Sodium bicarbonate Most organic solvents are denaturing, including:[citation needed] Ethanol Cross-linking agents ... sodium salicylate, dimethyl sulfoxide (DMSO), propylene glycol, and urea. These chemical denaturing agents lower the melting ... DMSO Formamide Guanidine Sodium salicylate Propylene glycol Urea Thermal denaturation Beads mill Probe sonication Radiation ...
... has part dodecyl sulfate (CHEBI:23872) sodium dodecyl sulfate (CHEBI:8984) has role ... sodium dodecyl sulfate (CHEBI:8984) has role protein denaturant (CHEBI:50533) sodium dodecyl sulfate (CHEBI:8984) is a alkyl ... CHEBI:8984 - sodium dodecyl sulfate. Main. ChEBI Ontology. Automatic Xrefs. Reactions. Pathways. Models. ... Sodium dodecyl sulfate KEGG COMPOUND sodium dodecyl sulphate ChemIDplus sodium dodecylsulfate ChemIDplus ...
Sodium dodecyl sulfate (SDS) or sodium lauryl sulfate (SLS), sometimes written sodium laurilsulfate, is a synthetic organic ... Sodium lauryl sulfate, in science referred to as sodium dodecyl sulfate (SDS), is used in cleaning procedures, and is commonly ... Sodium dodecyl sulfate, appearing as its synonym sodium lauryl sulfate (SLS), is considered a generally recognized as safe ( ... It consists of a 12-carbon tail attached to a sulfate group, that is, it is the sodium salt of dodecyl hydrogen sulfate, the ...
... português (Portugal). dansk (Danmark). čeština (Česká republika). français (Belgique). ...
For the whole category of alkyl sulfates (AS) a NOAEL of 488 mg/kg bw/day for oral and dermal exposure was established. ... The possibility of a read-across to other alkyl sulfates in accordance with Regulation (EC) No 1907/2006 Annex XI 1.5. Grouping ... This structural feature confers the surfactant properties of the alkyl sulfates. The surfactant property of the members of the ... A detailed justification for grouping of alkyl sulfates into a category is provided separately. Please refer for more details ...
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
Structure Changes in Sodium Dodecyl Sulfate Micelles Induced by Using Counterion Complexation by Macrocyclic Ligands. Author:. ...
other: Basic data given , limited information concerning test conditions and/or experimental methods Adopted according to OECD SIDS or other official EU regulation procedures (public available peer reviewed source). ...
Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate; Linear Formula: ... Sodium dodecyl sulfate ≥90%; CAS Number: 151-21-3; EC Number: 205-788-1; Synonym: ... Sodium dodecyl sulfate ≥90% Synonym: Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, ... Alkyl Sulfates, Anionic Detergents, Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, ...
Iron phosphate was prepared from iron nitrate and phosphoric acid with sodium dodecyl sulfate at various stirring hours. The ... When sodium dodecyl sulfate was added before pH adjustment, the mass of particles was formed. Therefore, sodium dodecyl sulfate ... and phosphoric acid with sodium dodecyl sulfate. The ratios of iron/cerium and sodium dodecyl sulfate/phosphorus were studied ... Sodium dodecyl sulfate (SDS) was added to the mixed solution in SDS/P = 0/10, 1/10, and 2/10 to prevent the aggregation of ...
Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate; Linear Formula: ... Sodium dodecyl sulfate BioXtra, ≥99.0% (GC); CAS Number: 151-21-3; EC Number: 205-788-1; Synonym: ... Sodium dodecyl sulfate BioXtra, ≥99.0% (GC) Synonym: Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium ... Sodium dodecyl sulfate has been used as a:. • surfactant to evaluate cytochrome c affinity by using surface plasmon resonance. ...
This chapter provides the diffusion coefficient of pentan-1-ol into dideuterium oxide and sodium dodecyl sulfate at different ... Winkelmann J. (2018) Diffusion coefficient of pentan-1-ol into dideuterium oxide and sodium dodecyl sulfate. In: Lechner M.D. ( ... This chapter provides the diffusion coefficient of pentan-1-ol into dideuterium oxide and sodium dodecyl sulfate at different ... Diffusion coefficient of pentan-1-ol into dideuterium oxide and sodium dodecyl sulfate. ...
Sodium Lauryl Sulfate, SDS
Shelf Life: 36 Months ... Sodium Dodecyl Sulfate Reagent. 25 g. Powder. Each (25g). ... Sodium Dodecyl Sulfate Laboratory. 100 g. Powder. Each (100g). Retrieving. Clearance . Act quickly! These items have limited ... Sodium Dodecyl Sulfate Laboratory. 500 g. Powder. Each (500g). Retrieving. Clearance . Act quickly! These items have limited ... Sodium Dodecyl Sulfate, Solution Laboratory. 50 mL. 10%. Each (50ml). Retrieving. Clearance . Act quickly! These items have ...
1NG8: The Structure, Cation Binding, Transport and Conductance of Gly15-Gramicidin a Incorporated Into Sds Micelles and Pc/Pg Vesicles
Influence of sodium dodecyl sulphate (SDS) on the inhibition efficiency of WHE-Zn2+ system When various concentrations of an ... Table 3. Influence of sodium dodecyl sulphate (SDS) on the inhibition efficiency of WHE-Zn2+ system.. WHE (mL) 0 8 8 8 8 8 8 ... A few drops of freshly prepared aluminium sulphate solution were mixed with the aqueous flower extract; aluminium flower ... copper and brass in acid chloride and sodium hydroxide solutions, using weight loss, solution analysis and potential ...
... buy latest sodium dodecyl sulfate sds direct from 27 sodium dodecyl sulfate sds Factories. ... Chemical Name : Sodium Lauryl Sulfate Synonyms: SLS, SDS, Sodium Dodecyl Sulfate,Sodium Lauryl Sulphate,Sodium Dodecyl Sulphate ... sodium dodecyl sulfate sds. All sodium dodecyl sulfate sds wholesalers & sodium dodecyl sulfate sds manufacturers come from ... SLS/K12 Other Names: Sodium lauryl sulphate, Sodium dodecyl sulfate, Sodium Laurilsulfate, dodecyl sulfate, sodium salt, SLS, ...
Effect of Long Chain Alcohols on Micellar Relaxation Time and Foaming Properties of Sodium Dodecyl Sulfate Solutions.. Patist A ... on the micellar stability of sodium dodecyl sulfate (SDS) solutions (SDS concentration ranging from 25 to 200 mM) was ...
Anionic surfactant, which will decrease polymer bead hydrophobicity and can additionally participate in charge stabilization of the suspension.
... sample purity Identifying disulfide bonds Quantifying proteins Blotting applications SDS-PAGE stands for sodium dodecyl (lauryl ... SDS-PAGE stands for sodium dodecyl (lauryl) sulfate-polyacrylamide gel electrophoresis. The SDS portion is a detergent. You may ...
India ink staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis and in conjunction with Western blots for ... peptide mapping of proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and ...
Structure of the bovine antimicrobial peptide indolicidin bound to dodecylphosphocholine and sodium dodecyl sulfate micelles. ... STRUCTURE OF THE BOVINE ANTIMICROBIAL PEPTIDE INDOLICIDIN BOUND TO SODIUM DODECYL SULFATE MICELLES. ...
In the resveratrol solubilization, sodium 3α,12α-dihydroxy-7-oxo-5β-cholanoate and sodium dodecyl sulfate exhibit different ... sodium-7-oxo-deoxycholate, S7-OD), (b) sodium dodecyl sulphate (SDS), and (c) resveratrol (trans-3,5,4′-trihydroxystilbene). ... Sodium dodecyl sulfate (SDS) is an aliphatic surfactant (Figure 1). SDS forms spherical micelles with a hydrophobic core [5, 17 ... The curve for sodium dodecyl sulfate was linear within the whole range of the investigated concentration; resveratrol ...
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in ... A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in ...
... Montalvo, G and Khan, ... Rheological properties of isotropic solutions and gel structures of lysozyme-sodium dodecyl sulfate mixtures in water are ... Rheological properties of isotropic solutions and gel structures of lysozyme-sodium dodecyl sulfate mixtures in water are ... Rheological properties of isotropic solutions and gel structures of lysozyme-sodium dodecyl sulfate mixtures in water are ...
Interactions between Hydrophobically Modified Alkali-Swellable Emulsion Polymers and Sodium Dodecyl Sulfate Probed by ... surfactant sodium dodecyl sulfate Newtonian plateau regions SDS concentrations fluorescence spectra MF SDS concentration 4 ... Interactions between Hydrophobically Modified Alkali-Swellable Emulsion Polymers and Sodium Dodecyl Sulfate Probed by ... polymer and the anionic surfactant sodium dodecyl sulfate (SDS) in aqueous solution were investigated with a fluorometer, a ...
Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting. CLODAGH ... Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting ... Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting ... Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting ...
"Photooxidative decomposition of sodium dodecyl sulfate in aqueous solutions, Russian Journal of Applied Chemistry" on DeepDyve ... Photooxidative decomposition of sodium dodecyl sulfate in aqueous solutions. Photooxidative decomposition of sodium dodecyl ... Photooxidative decomposition of sodium dodecyl sulfate in aqueous solutions. Panich, N.; Seliverstov, A.; Ershov, B. ... Kinetic features and the mechanism of photooxidative decomposition of sodium dodecyl sulfate in an aqueous solution under the ...
Anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. Applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain. As a result, the distance of migration through the gel in SDS-PAGE electrophoresis can be assumed to be directly related only to the size of the protein. More than 99% pure. One tablet weighs 0.5 g and can be dissolved in deionized water to the desired volume.
Check out our best-in-class prices for Dust-Free Sodium Dodecyl Sulfate at AG Scientific, Inc. With more than 20 years of ... Sodium dodecyl sulfate (SDS) is an organic salt and an anionic surfactant that is widely used in biochemical research due to ... Dust-Free SDS (Sodium Dodecyl Sulfate) is an anionic detergent. It is used in chromatin immunoprecipitation and in SDS- ... S-2066, DUST FREE-SDS, SODIUM DODECEYL SULFATE, SDS, diamond format.pdf ...
Effect of Sodium Dodecyl Sulphate on Protonation Equilibrium of (Salicylato)bis(ethylenediamine)cobalt(III): Test of Validity ...
3-sodium dodecyl sulfate-isooctyl alcohol, Russian Journal of Applied Chemistry" on DeepDyve, the largest online rental service ... 3-sodium dodecyl sulfate-isooctyl alcohol. Research of extraction in system Ln(NO3)3-sodium dodecyl sulfate-isooctyl alcohol ... Research of extraction in system Ln(NO3)3-sodium dodecyl sulfate-isooctyl alcohol. Chirkst, D.; Lobacheva, O.; Dzhevaga, N. ... lp/springer_journal/research-of-extraction-in-system-ln-no3-3-sodium-dodecyl-sulfate-7KPbSTJMGk ...
  • The cap-forming subpopulation was found to develop tolerance to membrane-targeting antimicrobial agents, such as the cyclic cationic peptide colistin and the detergent sodium dodecyl sulfate. (asm.org)
  • Dust-Free SDS (Sodium Dodecyl Sulfate) is an anionic detergent. (agscientific.com)
  • Sodium dodecyl sulfate (SDS) is an anionic detergent that is used for the solubilization and denaturation of proteins. (takarabio.com)
  • Sodium Dodecyl Sulfate (SDS) is an anionic detergent that denatures protein structures. (takarabio.com)
  • Sodium dodecyl sulphate (SDS) is an anionic detergent which denatures proteins by 'wrapping around' the polypeptide backbone - and SDS binds to proteins fairly specifically in a mass ratio of 1.4:1. (uct.ac.za)
  • Sodium Dodecyl Sulfate (SDS) is an ionic detergent used to denature proteins. (hica.co.za)
  • Sodium Dodecyl Sulfate, Molecular Biology Grade (SDS), is a detergent that is known to denature proteins. (aushealthingredients.com)
  • Sodium lauryl sulfate, in science referred to as sodium dodecyl sulfate (SDS), is used in cleaning procedures, and is commonly used as a component for lysing cells during RNA extraction and/or DNA extraction, and for denaturing proteins in preparation for electrophoresis in the SDS-PAGE technique. (wikipedia.org)
  • Sodium dodecyl sulfate (SDS) is an anionic surfactant with an ability to denature proteins. (sigmaaldrich.com)
  • We present an approach that allows matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOFMS) peptide mapping of proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotted onto nitrocellulose (NC). (nih.gov)
  • Sodium dodecyl sulfate (SDS) is an organic salt and an anionic surfactant that is widely used in biochemical research due to its ability to disrupt cell membranes and increase the solubility of proteins. (agscientific.com)
  • Identification and classification of lactic acid bacteria by sodium dodecyl sulphate polyacrylamide gel-electrophoresis of whole-cell proteins. (ugent.be)
  • Pot B, Kersters K. Identification and classification of lactic acid bacteria by sodium dodecyl sulphate polyacrylamide gel-electrophoresis of whole-cell proteins. (ugent.be)
  • In proteomics, one-dimensional (1D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is broadly used for protein fractionation previous to mass spectrometric evaluation to boost the dynamic vary of evaluation and to enhance the identification of low-abundance proteins. (westernblotinfo.com)
  • The binding of sodium dodecyl sulphate to various proteins. (wizdom.ai)
  • The sulfhydryl groups that could be titrated in the intact proteins in sodium dodecyl sulfate were on the A chains. (elsevier.com)
  • The temperature-dependent migration of molecular weight protein size standards and several biotherapeutic proteins were studied in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) in the interval from 15 to 60 °C using borate cross-linked dextran sieving matrix. (uni-pannon.hu)
  • SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis), is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa. (wikipedia.org)
  • The combined use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel allows to eliminate the influence of structure and charge, and proteins are separated solely on the basis of differences in their molecular weight. (wikipedia.org)
  • citation needed] When produced from these sources, commercial samples of these "SDS" products are actually not pure SDS, rather a mixture of various sodium alkyl sulfates with SDS being the main component. (wikipedia.org)
  • For the whole category of alkyl sulfates (AS) a NOAEL of 488 mg/kg bw/day for oral and dermal exposure was established. (europa.eu)
  • For the whole category of alkyl sulfates (AS) a NOAEL of 488 mg/kg bw/day was established. (europa.eu)
  • The possibility of a read-across to other alkyl sulfates in accordance with Regulation (EC) No 1907/2006 Annex XI 1.5. (europa.eu)
  • This structural feature confers the surfactant properties of the alkyl sulfates. (europa.eu)
  • A detailed justification for grouping of alkyl sulfates into a category is provided separately. (europa.eu)
  • Therefore this endpoint is covered by read across to structurally related alkyl sulfates. (europa.eu)
  • Hence, alkyl sulfates with chain lengths between C10 and C18 have been tested. (europa.eu)
  • The most commonly used alkyl sulfates. (surfactant.top)
  • In this work, as an additive, sodium dodecyl sulfate (SDS) was used to prevent the aggregation in preparation process, which was one of the common anionic surfactant. (hindawi.com)
  • Sodium dodecyl sulfate is a kind of anionic surfactant, belongs to the typical representative of sulfuric acid ester surfactant, SDS for short, also known AS, K12, coconut oil, alcohol, sodium sulfate , lauryl sodium sulfate , foaming. (spintoband.com)
  • The interactions between a pyrene-labeled hydrophobically modified alkali-swellable emulsion (Py-HASE) polymer and the anionic surfactant sodium dodecyl sulfate (SDS) in aqueous solution were investigated with a fluorometer, a rheometer, and a combination of both instruments to probe the fluorescence of the polymer while the solution was being sheared. (figshare.com)
  • In this work, results from atomistic molecular dynamics studies investigating the effect of surfactant concentration on the transport properties of bulk surfactant aqueous solutions, focusing on the anionic surfactant sodium dodecyl sulfate (SDS), are reported. (asme.org)
  • Sodium Lauryl Sulfate (SLS) is an anionic surfactant naturally derived from coconut and/or palm kernel oil. (hica.co.za)
  • Effect of sodium dodecyl sulphate. (semanticscholar.org)
  • 1395). 'Effect of Sodium Dodecyl Sulphate on Gas Hydrate Formation Kinetics ‎of Methane and Ethane Mixtures', سامانه مدیریت نشریات علمی دانشگاه اصفهان , 5(1), pp. 65-74. (ac.ir)
  • Sigma-Aldrich offers Aldrich-451851, Sodium dodecyl-d25 sulfate for your research needs. (hica.co.za)
  • Structure Changes in Sodium Dodecyl Sulfate Micelles Induced by Using Counterion Complexation by Macrocyclic Ligands. (europa.eu)
  • Also, we studied resveratrol behavior in mixed micelles with mentioned surfactants using spectroflourimetric method as well as the effects of sodium chloride and urea on resveratrol solubilization capacity in the applied surfactants. (hindawi.com)
  • this surface charge increased the repulsive forces between the dodecyl sulfate ions, and promoted the dispersion of the micelles. (pku.edu.cn)
  • It consists of a 12-carbon tail attached to a sulfate group, that is, it is the sodium salt of dodecyl hydrogen sulfate, the ester of dodecyl alcohol and sulfuric acid. (wikipedia.org)
  • Sodium salt of 3 α ,12 α -dihydroxy-7-oxo-5 β -cholanoic acid (S7-OD) is oxo derivative of cholic acid (Figure 1 ). (hindawi.com)
  • Dzhevaga, N. 2013-03-15 00:00:00 Extraction in a system sodium dodecyl sulfate-aqueous solution of a lanthanide salt-isooctyl alcohol was examined in the study. (deepdyve.com)
  • Kami Menjual Dodecyl sulfate sodium salt for biochemistry and surfactant tests untuk Wilayah Medan, Bali, Bandung, Jember, Pontianak dan Wilayah lainnya. (jualbahankimia.net)
  • Sodium Dodecyl Sulfate SDS CAS: 151-21-3 Items 95% Appearance, 25 °C White to yellowish powder or needle Active matter, % 95 Min. (spintoband.com)
  • Sodium Dodecyl Sulfate (SDS) 20% Powder (Cat. (takarabio.com)
  • Sodium dodecyl sulfate , white or light yellow powder, is insensitive to alkali and hard water. (zgshenyu.com)
  • Also known as Dodecyl Lithium Sulfate or Lithium Lauryl Sulfate, LDS and SDS are sold both in research and bulk containers. (reliablebiopharm.com)
  • SDS-PAGE stands for sodium dodecyl (lauryl) sulfate-polyacrylamide gel electrophoresis. (asu.edu)
  • India ink staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis and in conjunction with Western blots for peptide mapping by mat. (nih.gov)
  • India ink staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis and in conjunction with Western blots for peptide mapping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (nih.gov)
  • Characterization of sodium dodecyl sulfate-stable Staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis. (asm.org)
  • Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: stable association of lipopolysaccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae. (asm.org)
  • Use of DNA ladders for reproducible protein fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantitative proteomics. (westernblotinfo.com)
  • Sodium DodecylSulfate PolyAcrylamide Gel Electrophoresis (which takes up half a Tweet's worth of space on its own :P) is one of the most frequently used techniques in biochemistry. (thebumblingbiochemist.com)
  • Measurements of differential scanning calorimetry (d.s.c.) have been made on the complex bovine serum albumin (BSA)--sodium dodecyl sulphate (SDS) under various conditions. (semanticscholar.org)
  • The binding isotherms of the interaction between bovine serum albumin (BSA) and sodium dodecyl sulfate (SDS) were obtained using electromotive force measurements. (pku.edu.cn)
  • Mechanism of Interaction between Bovine Serum Albumin and Sodium Dodecyl Sulfate[J].Acta Phys. (pku.edu.cn)
  • In this study, the effects of sodium dodecyl sulphate (SDS) on gas hydrate formation kinetics of methane and ethane mixture has been investigated experimentally and also the thermodynamic natural path has been used for modeling of the hydrate formation rate in a constant volume process. (ac.ir)
  • Sodium dodecyl sulfate, appearing as its synonym sodium lauryl sulfate (SLS), is considered a generally recognized as safe (GRAS) ingredient for food use according to the USFDA (21 CFR 172.822). (wikipedia.org)
  • Further controlled studies using MB/hexadecyltrimethylammonium bromide, MB/sodium octyl sulfate, and MB/sodium chloride showed that the binding between MB and SDS occurred at the sulfate moiety of SDS. (spie.org)
  • Surfactant Behavior of Sodium Dodecylsulfate in Deep Eutectic Solvent Choline Chloride/Urea. (lu.se)
  • Here we present some results that demonstrate that surfactant sodium dodecyl sulfate (SDS) remains surface-active and shows self-assembly phenomena in the most commonly studied DES, choline chloride/urea. (lu.se)
  • Ershov, B. 2009-02-11 00:00:00 Kinetic features and the mechanism of photooxidative decomposition of sodium dodecyl sulfate in an aqueous solution under the action of pulsed short-wavelength UV light with a continuous spectrum upon introduction of hydrogen peroxide was studied. (deepdyve.com)
  • Dynamic Surface Adsorption Properties of Sodium Dodecyl Sulfate Aqueous Solution[J].Acta Phys. (pku.edu.cn)
  • Iron phosphate was prepared from iron nitrate and phosphoric acid with sodium dodecyl sulfate at various stirring hours. (hindawi.com)
  • The phosphate particles were dispersed in the solution by the addition of sodium dodecyl sulfate after pH adjustment. (hindawi.com)
  • Reagecon's Potassium Phosphate pH 6.8 and 0.5% Sodium Dodecyl Sulphate Dissolution Media Concentrate is presented in concentrate form. (reagecon.com)
  • Profiles of the bacteriolytic activities of Staphylococcus aureus culture supernatants, sodium dodecyl sulfate cell extracts, LiCl cell extracts, cell wall extracts, and cell membranes were analyzed in sodium dodecyl sulfate-polyacrylamide gels containing Micrococcus luteus or S. aureus. (asm.org)
  • Lloyd, MD 1996, ' Fast staining and destaining of sodium dodecyl sulfate-polyacrylamide gels ', Analytical Biochemistry , vol. 241, no. 1, pp. 139-140. (bath.ac.uk)
  • The effect of amino acid (glycine) on mesomorphic properties of the sodium dodecyl sulfate - glycine - water system, wherein sodium dodecylsulphate was used as a material simulating liquid crystal structure of a biological membrane, was established. (ac.ru)
  • The data on the interaction energy of sodium dodecyl sulfate with water and glycine, obtained by quantum-chemical modeling, allowed to theoretically justify a shift in the formation of hexagonal lyomesophase to lower concentrations of the surfactant of the studied ternary system compared with the binary system of sodium alkylsulfate - water. (ac.ru)
  • The resulting product is then neutralized through the addition of sodium hydroxide or sodium carbonate. (wikipedia.org)
  • SDS is synthesized by treating lauryl alcohol with sulfur trioxide gas, oleum, or chlorosulfuric acid to produce hydrogen lauryl sulfate. (wikipedia.org)
  • Sodium dodecyl sulfate price,Sodium dodecyl sulfate suppliers,Sodium dodecyl sulfate manufacturer-【Bolise Co., Ltd. (aushealthingredients.com)
  • Solubility soluble in water, solubility in water is less than sodium laureth sulfate. (surfactant.top)
  • article{557bca77-5212-4dbe-a6dc-0bf47b4bfa7e, abstract = {Rheological properties of isotropic solutions and gel structures of lysozyme-sodium dodecyl sulfate mixtures in water are investigated. (lu.se)
  • Abstract Molecular dynamics simulations have been performed on the fully hydrated lipid bilayer with different concentrations of sodium dodecyl sulfate (SDS). (iphy.ac.cn)
  • Visit ChemicalBook To find more Sodium dodecyl sulfate (151-21-3) information like chemical properties,Structure,melting point,boiling point,density,molecular. (hica.co.za)
  • Cawley, DB & Houston, LL 1979, ' Effect of sulfhydryl reagents and protease inhibitors on sodium dodecyl sulfate-heat induced dissociation of Ricinus communis agglutinin ', BBA - Protein Structure , vol. 581, no. 1, pp. 51-62. (elsevier.com)
  • Under acidic condition, hydrolytic stability is less than sodium dodecyl sulfonate, similar to sodium laureth sulfate. (surfactant.top)
  • Irritation of sodium dodecyl sulfate is higher than sodium laureth sulfate, lower than sodium dodecylbenzene sulfonate. (surfactant.top)
  • Customer who searched sodium dodecylbenzen sulfonate also searched: ammonium sulfonate, sodium alpha-olefin sulfonate/aos, sodium hydroxy methylene sulfonate. (hica.co.za)
  • sodium dodecyl sulfonate_sodium dodecyl sulfo. (hica.co.za)
  • The interaction of methylene blue (MB) and sodium dodecyl sulfate (SDS) leads to a reversible spectral shift during SDS micellization, but the underlying mechanism has remained unclear. (spie.org)
  • The effect of long chain alcohols (5 mol% CnOH for n = 8, 10, 12, 14, and 16) on the micellar stability of sodium dodecyl sulfate (SDS) solutions (SDS concentration ranging from 25 to 200 mM) was investigated and related to foaming properties, such as foamability, dynamic and equilibrium surface tension, and surface viscosity. (nih.gov)
  • Resveratrol solubilization curve for sodium 3 α ,12 α -dihydroxy-7-oxo-5 β -cholanoate at concentration 0.9 CMC reached saturation level of 60% dissolved resveratrol. (hindawi.com)
  • Reference[6]: goldfish absorb sodium dodecyl sulfate from solution principally through the gills, and that sodium dodecyl sulfate is rapidly distributed throughout the body tissues, the highest concentration being found in the gall bladder. (surfactant.top)
  • 3), In hard water, there is a possibility that sodium lauryl sulfate solution is transparent in a concentration range, then transforms translucent state. (surfactant.top)
  • At the same time, when concentration of sodium lauryl sulfate is higher than or lower than this concentration, the solution is transparent. (surfactant.top)
  • As a case study, synthesize of sodium dodecyl isosorbide sulfates showed 5-0 dodecyl isosorbide sulfate as it's isomer is more efficient surfactant in comparison with 2-O-dodecyl isosorbide sulfate and It hold in fee simple a much lower critical micelle concentration (CMC) than sodium dodecyl sulfate. (getpaper.ir)
  • Sodium dodecyl sulfate (SDS) or sodium lauryl sulfate (SLS), sometimes written sodium laurilsulfate, is a synthetic organic compound with the formula CH3(CH2)11SO4Na. (wikipedia.org)
  • This chapter provides the diffusion coefficient of propan-1-ol into dideuterium oxide and sodium dodecyl sulfate at different concentrations measured using NMR spin echo pulse gradient. (springer.com)
  • The dynamic surface adsorption properties of aqueous sodium dodecyl sulfate (SDS) solutions were investigated at different concentrations of NaCl using bubble pressure tensiometry MPTC. (pku.edu.cn)
  • Not only can we supply you with the Dodecyl Sodium Sulfate you are looking for, but in the size you require, at a very competitive price. (alphachem.ca)
  • Results of interaction between cetyltrimethylammonium bromide(CTAB) and sodium dodecylsulfate(SDS) at levels below and above their critical micelle concentrations (CMCs) in aqueous medium and in the presence of additives like NaCl, glucose, urea, p-tert-alkyl-phenoxypolyoxyethyleneether(Triton X 100), polyoxyethylenesorbitanmonolaurate(Tween 20), polyoxyethylenesorbitanmonooleate(Tween 80) and polyethyleneglycol (M.W. 600, PEG 600) are presented. (springer.com)
  • In this study, polarization curves were made under non-toxic conditions and under toxic conditions after the addition of various concentrations of nickel, bentazon, sodiumdodecyl sulfate and potassium ferricyanide. (mdpi.com)
  • Ionic detergents like sodium dodecyl sulfate (SDS) and cationic detergents like ethyl trimethyl ammonium bromide are denaturing (will disrupt protein functions). (wikipedia.org)
  • Anhydrous sodium sulfate is the most common drying agent used to remove moisture from sample extracts. (thomassci.com)
  • Affinity labeling of the acetylcholine receptor in the electroplax: electrophoretic separtion in sodium dodecyl sulfate. (elsevier.com)
  • T2 - electrophoretic separtion in sodium dodecyl sulfate. (elsevier.com)
  • Ricinus communis agglutinin dissociated to lower molecular weight forms when heated in sodium dodecyl sulfate in the absence of reducing agents, while ricin was little affected by such treatment. (elsevier.com)
  • Winkelmann J. (2018) Diffusion coefficient of propan-1-ol into dideuterium oxide and sodium dodecyl sulfate. (springer.com)
  • In this work, iron-cerium phosphates were prepared form iron nitrate, ammonium cerium nitrate, and phosphoric acid with sodium dodecyl sulfate. (hindawi.com)
  • Sodium dodecyl sulfate was metabolised by the fish to butyric acid-4-sulphate, and was excreted by the kidney. (surfactant.top)
  • amresco sodium dodecyl sulfate msds double-barrelled Rudolfo splatter it attaints marinating jeeringly. (gooog.tk)
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