A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Deoxyribonucleic acid that makes up the genetic material of viruses.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Viruses whose genetic material is RNA.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Established cell cultures that have the potential to propagate indefinitely.
The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.
Process of growing viruses in live animals, plants, or cultured cells.
The functional hereditary units of VIRUSES.
Substances elaborated by viruses that have antigenic activity.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Proteins found in any species of virus.
The process by which a DNA molecule is duplicated.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
The expelling of virus particles from the body. Important routes include the respiratory tract, genital tract, and intestinal tract. Virus shedding is an important means of vertical transmission (INFECTIOUS DISEASE TRANSMISSION, VERTICAL).
A general term for diseases produced by viruses.
Ribonucleic acid that makes up the genetic material of viruses.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
Viruses parasitic on plants higher than bacteria.
Viruses whose nucleic acid is DNA.
The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.
A species of POLYOMAVIRUS apparently infecting over 90% of children but not clearly associated with any clinical illness in childhood. The virus remains latent in the body throughout life and can be reactivated under certain circumstances.
The type species of MORBILLIVIRUS and the cause of the highly infectious human disease MEASLES, which affects mostly children.
A subtype of INFLUENZA A VIRUS with the surface proteins hemagglutinin 1 and neuraminidase 1. The H1N1 subtype was responsible for the Spanish flu pandemic of 1918.
A genus of potentially oncogenic viruses of the family POLYOMAVIRIDAE. These viruses are normally present in their natural hosts as latent infections. The virus is oncogenic in hosts different from the species of origin.
A family of small, non-enveloped DNA viruses, infecting mainly MAMMALS, and containing a single genus: POLYOMAVIRUS.
The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 5 and neuraminidase 1. The H5N1 subtype, frequently referred to as the bird flu virus, is endemic in wild birds and very contagious among both domestic (POULTRY) and wild birds. It does not usually infect humans, but some cases have been reported.
A species of RUBULAVIRUS originally isolated from cultured primary monkey cells. Its natural host is the DOG in which it causes kennel cough, but it can also infect humans.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.
The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the virions have both HEMAGGLUTININ and NEURAMINIDASE activities and encode a non-structural C protein. SENDAI VIRUS is the type species.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 3 and neuraminidase 2. The H3N2 subtype was responsible for the Hong Kong flu pandemic of 1968.
A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE). It can infect birds and mammals. In humans, it is seen most frequently in Africa, Asia, and Europe presenting as a silent infection or undifferentiated fever (WEST NILE FEVER). The virus appeared in North America for the first time in 1999. It is transmitted mainly by CULEX spp mosquitoes which feed primarily on birds, but it can also be carried by the Asian Tiger mosquito, AEDES albopictus, which feeds mainly on mammals.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The mechanism by which latent viruses, such as genetically transmitted tumor viruses (PROVIRUSES) or PROPHAGES of lysogenic bacteria, are induced to replicate and then released as infectious viruses. It may be effected by various endogenous and exogenous stimuli, including B-cell LIPOPOLYSACCHARIDES, glucocorticoid hormones, halogenated pyrimidines, IONIZING RADIATION, ultraviolet light, and superinfecting viruses.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A group of viruses in the PNEUMOVIRUS genus causing respiratory infections in various mammals. Humans and cattle are most affected but infections in goats and sheep have also been reported.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the species have hemagglutinin and neuraminidase activities but lack a C protein. MUMPS VIRUS is the type species.
Viruses that produce tumors.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A species of RESPIROVIRUS also called hemadsorption virus 2 (HA2), which causes laryngotracheitis in humans, especially children.
A species of POLYOMAVIRUS, originally isolated from the brain of a patient with progressive multifocal leukoencephalopathy. The patient's initials J.C. gave the virus its name. Infection is not accompanied by any apparent illness but serious demyelinating disease can appear later, probably following reactivation of latent virus.
A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.
The type species of VESICULOVIRUS causing a disease symptomatically similar to FOOT-AND-MOUTH DISEASE in cattle, horses, and pigs. It may be transmitted to other species including humans, where it causes influenza-like symptoms.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Membrane glycoproteins from influenza viruses which are involved in hemagglutination, virus attachment, and envelope fusion. Fourteen distinct subtypes of HA glycoproteins and nine of NA glycoproteins have been identified from INFLUENZA A VIRUS; no subtypes have been identified for Influenza B or Influenza C viruses.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A family of spherical viruses, of the order MONONEGAVIRALES, somewhat larger than the orthomyxoviruses, and containing single-stranded RNA. Subfamilies include PARAMYXOVIRINAE and PNEUMOVIRINAE.
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
The ability of a pathogenic virus to lie dormant within a cell (latent infection). In eukaryotes, subsequent activation and viral replication is thought to be caused by extracellular stimulation of cellular transcription factors. Latency in bacteriophage is maintained by the expression of virally encoded repressors.
The type species of RUBULAVIRUS that causes an acute infectious disease in humans, affecting mainly children. Transmission occurs by droplet infection.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Group of alpharetroviruses (ALPHARETROVIRUS) producing sarcomata and other tumors in chickens and other fowl and also in pigeons, ducks, and RATS.
Species of the genus LENTIVIRUS, subgenus primate immunodeficiency viruses (IMMUNODEFICIENCY VIRUSES, PRIMATE), that induces acquired immunodeficiency syndrome in monkeys and apes (SAIDS). The genetic organization of SIV is virtually identical to HIV.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Infections with POLYOMAVIRUS, which are often cultured from the urine of kidney transplant patients. Excretion of BK VIRUS is associated with ureteral strictures and CYSTITIS, and that of JC VIRUS with progressive multifocal leukoencephalopathy (LEUKOENCEPHALOPATHY, PROGRESSIVE MULTIFOCAL).
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Viruses which produce a mottled appearance of the leaves of plants.
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.
Proteins that form the CAPSID of VIRUSES.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A species in the genus HEPATOVIRUS containing one serotype and two strains: HUMAN HEPATITIS A VIRUS and Simian hepatitis A virus causing hepatitis in humans (HEPATITIS A) and primates, respectively.
A species of ALPHAVIRUS isolated in central, eastern, and southern Africa.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A family of RNA viruses causing INFLUENZA and other diseases. There are five recognized genera: INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; ISAVIRUS; and THOGOTOVIRUS.
The relationships of groups of organisms as reflected by their genetic makeup.
Viruses whose taxonomic relationships have not been established.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The sum of the weight of all the atoms in a molecule.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
Proteins conjugated with nucleic acids.
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
The type species of ALPHARETROVIRUS producing latent or manifest lymphoid leukosis in fowl.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The type species of ORBIVIRUS causing a serious disease in sheep, especially lambs. It may also infect wild ruminants and other domestic animals.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Virus diseases caused by the ORTHOMYXOVIRIDAE.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
Specific hemagglutinin subtypes encoded by VIRUSES.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The type species of RESPIROVIRUS in the subfamily PARAMYXOVIRINAE. It is the murine version of HUMAN PARAINFLUENZA VIRUS 1, distinguished by host range.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Insertion of viral DNA into host-cell DNA. This includes integration of phage DNA into bacterial DNA; (LYSOGENY); to form a PROPHAGE or integration of retroviral DNA into cellular DNA to form a PROVIRUS.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
The type species of the FLAVIVIRUS genus. Principal vector transmission to humans is by AEDES spp. mosquitoes.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
A species of RESPIROVIRUS frequently isolated from small children with pharyngitis, bronchitis, and pneumonia.
Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.
The type species of TOBAMOVIRUS which causes mosaic disease of tobacco. Transmission occurs by mechanical inoculation.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Pneumovirus infections caused by the RESPIRATORY SYNCYTIAL VIRUSES. Humans and cattle are most affected but infections in goats and sheep have been reported.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The type species of LEPORIPOXVIRUS causing infectious myxomatosis, a severe generalized disease, in rabbits. Tumors are not always present.
Inactivation of viruses by non-immune related techniques. They include extremes of pH, HEAT treatment, ultraviolet radiation, IONIZING RADIATION; DESICCATION; ANTISEPTICS; DISINFECTANTS; organic solvents, and DETERGENTS.
The rate dynamics in chemical or physical systems.
A species of ORTHOPOXVIRUS that is the etiologic agent of COWPOX. It is closely related to but antigenically different from VACCINIA VIRUS.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A species of ORTHOPOXVIRUS causing infections in humans. No infections have been reported since 1977 and the virus is now believed to be virtually extinct.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions.
Sites on an antigen that interact with specific antibodies.
The type species of PNEUMOVIRUS and an important cause of lower respiratory disease in infants and young children. It frequently presents with bronchitis and bronchopneumonia and is further characterized by fever, cough, dyspnea, wheezing, and pallor.
A species of ARENAVIRUS, part of the Old World Arenaviruses (ARENAVIRUSES, OLD WORLD), and the etiologic agent of LASSA FEVER. LASSA VIRUS is a common infective agent in humans in West Africa. Its natural host is the multimammate mouse Mastomys natalensis.
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
A species of ALPHAVIRUS causing an acute dengue-like fever.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The type species in the genus NOROVIRUS, first isolated in 1968 from the stools of school children in Norwalk, Ohio, who were suffering from GASTROENTERITIS. The virions are non-enveloped spherical particles containing a single protein. Multiple strains are named after the places where outbreaks have occurred.
An acute viral infection in humans involving the respiratory tract. It is marked by inflammation of the NASAL MUCOSA; the PHARYNX; and conjunctiva, and by headache and severe, often generalized, myalgia.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
A group of replication-defective viruses, in the genus GAMMARETROVIRUS, which are capable of transforming cells, but which replicate and produce tumors only in the presence of Murine leukemia viruses (LEUKEMIA VIRUS, MURINE).
A collection of single-stranded RNA viruses scattered across the Bunyaviridae, Flaviviridae, and Togaviridae families whose common property is the ability to induce encephalitic conditions in infected hosts.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Biological properties, processes, and activities of VIRUSES.
Infection with human herpesvirus 4 (HERPESVIRUS 4, HUMAN); which may facilitate the development of various lymphoproliferative disorders. These include BURKITT LYMPHOMA (African type), INFECTIOUS MONONUCLEOSIS, and oral hairy leukoplakia (LEUKOPLAKIA, HAIRY).
An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A subgroup of the genus FLAVIVIRUS that causes encephalitis and hemorrhagic fevers and is found in eastern and western Europe and the former Soviet Union. It is transmitted by TICKS and there is an associated milk-borne transmission from viremic cattle, goats, and sheep.
A species of GAMMARETROVIRUS causing leukemia, lymphosarcoma, immune deficiency, or other degenerative diseases in cats. Several cellular oncogenes confer on FeLV the ability to induce sarcomas (see also SARCOMA VIRUSES, FELINE).
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS).
Actual loss of portion of a chromosome.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
The type species of APHTHOVIRUS, causing FOOT-AND-MOUTH DISEASE in cloven-hoofed animals. Several different serotypes exist.
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Proteins prepared by recombinant DNA technology.
Antibodies produced by a single clone of cells.
A species of ARTERIVIRUS causing reproductive and respiratory disease in pigs. The European strain is called Lelystad virus. Airborne transmission is common.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Any of the viruses that cause inflammation of the liver. They include both DNA and RNA viruses as well viruses from humans and animals.
Elements of limited time intervals, contributing to particular results or situations.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Glycoprotein from Sendai, para-influenza, Newcastle Disease, and other viruses that participates in binding the virus to cell-surface receptors. The HN protein possesses both hemagglutinin and neuraminidase activity.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
The type species of PARAPOXVIRUS which causes a skin infection in natural hosts, usually young sheep. Humans may contract local skin lesions by contact. The virus apparently persists in soil.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
A group of viruses in the genus PESTIVIRUS, causing diarrhea, fever, oral ulcerations, hemorrhagic syndrome, and various necrotic lesions among cattle and other domestic animals. The two species (genotypes), BVDV-1 and BVDV-2 , exhibit antigenic and pathological differences. The historical designation, BVDV, consisted of both (then unrecognized) genotypes.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
A positive-stranded RNA virus species in the genus HEPEVIRUS, causing enterically-transmitted non-A, non-B hepatitis (HEPATITIS E).
A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) producing leukemia of the reticulum-cell type with massive infiltration of liver, spleen, and bone marrow. It infects DBA/2 and Swiss mice.
The type species of BETARETROVIRUS commonly latent in mice. It causes mammary adenocarcinoma in a genetically susceptible strain of mice when the appropriate hormonal influences operate.
An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.
Infections with viruses of the genus RESPIROVIRUS, family PARAMYXOVIRIDAE. Host cell infection occurs by adsorption, via HEMAGGLUTININ, to the cell surface.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Viruses whose hosts are in the domain ARCHAEA.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 7 and neuraminidase 7. The H7N7 subtype produced an epidemic in 2003 which was highly pathogenic among domestic birds (POULTRY). Some infections in humans were reported.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
Genotypic differences observed among individuals in a population.
Stable phosphorus atoms that have the same atomic number as the element phosphorus, but differ in atomic weight. P-31 is a stable phosphorus isotope.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The type species of the genus AVIPOXVIRUS. It is the etiologic agent of FOWLPOX.
The quantity of measurable virus in a body fluid. Change in viral load, measured in plasma, is sometimes used as a SURROGATE MARKER in disease progression.

Human topoisomerase I promotes initiation of simian virus 40 DNA replication in vitro. (1/4150)

Addition of purified human topoisomerase I (topo I) to simian virus 40 T antigen-driven in vitro DNA replication reactions performed with topo I-deficient extracts results in a greater than 10-fold stimulation of completed molecules as well as a more than 3-fold enhancement of overall DNA replication. To further characterize this stimulation, we first demonstrate that bovine topo I but not Escherichia coli topo I can also enhance DNA replication. By using several human topo I mutants, we show that a catalytically active form of topo I is required. To delineate whether topo I influences the initiation or the elongation step of replication, we performed delayed pulse, pulse-chase, and delayed pulse-chase experiments. The results illustrate that topo I cannot promote the completion of partially replicated molecules but is needed from the beginning of the reaction to initiate replication. Competitive inhibition experiments with the topo I binding T antigen fragment 1-246T and a catalytically inactive topo I mutant suggest that part of topo I's stimulation of replication is mediated through a direct interaction with T antigen. Collectively, our data indicate that topo I enhances the synthesis of fully replicated DNA molecules by forming essential interactions with T antigen and stimulating initiation.  (+info)

Induction of AT-specific DNA-interstrand crosslinks by bizelesin in genomic and simian virus 40 DNA. (2/4150)

Bizelesin is a bifunctional AT-specific DNA alkylating drug. Our study characterized the ability of bizelesin to induce interstrand crosslinks, a potential lethal lesion. In genomic DNA of BSC-1 cells, bizelesin formed from approx. 0.3 to 6.03+/-0.85 interstrand crosslinks per 106 base pairs, at 5-100 nM drug concentration, respectively, comparable to the number of total adducts previously determined in the same system (J.M. Woynarowski, M.M. McHugh, L.S. Gawron, T.A. Beerman, Biochemistry 34 (1995) 13042-13050). Bizelesin did not induce DNA-protein crosslinks or strand breaks. A model defined target, intracellular simian virus 40 (SV40) DNA, was employed to map at the nucleotide level sites of bizelesin adducts, including potential interstrand crosslinks. Preferential adduct formation was observed at AT tracts which are abundant in the SV40 matrix associated region and the origin of replication. Many sites, including each occurrence of 5'-T(A/T)4A-3', co-mapped on both DNA strands suggesting interstrand crosslinks, although monoadducts were also formed. Bizelesin adducts in naked SV40 DNA were found at similar sites. The localization of bizelesin-induced crosslinks in AT-rich tracts of replication-related regions is consistent with the potent anti-replicative properties of bizelesin. Given the apparent lack of other types of lesions in genomic DNA, interstrand crosslinks localized in AT-rich tracts, and to some extent perhaps also monoadducts, are likely to be lethal effects of bizelesin.  (+info)

Downregulation of metallothionein-IIA expression occurs at immortalization. (3/4150)

Metallothioneins (MTs) may modulate a variety of cellular processes by regulating the activity of zinc-binding proteins. These proteins have been implicated in cell growth regulation, and their expression is abnormal in some tumors. In particular, MT-IIA is expressed 27-fold less in human colorectal tumors and tumor cell lines compared with normal tissue (Zhang et al., 1997). Here we demonstrate that MT-IIA downregulation occurs when human cells become immortal, a key event in tumorigenesis. After immortalization MT-IIA expression remains inducible but the basal activity of the MT-IIA promoter is decreased. MT-IIA downregulation at immortalization is one of the most common immortalization-related changes identified to date, suggesting that MT-IIA has a role in this process.  (+info)

Association of simian virus 40 with a central nervous system lesion distinct from progressive multifocal leukoencephalopathy in macaques with AIDS. (4/4150)

The primate polyomavirus SV40 is known to cause interstitial nephritis in primary infections and progressive multifocal leukoencephalopathy (PML) upon reactivation of a latent infection in SIV-infected macaques. We now describe a second central nervous system manifestation of SV40: a meningoencephalitis affecting cerebral gray matter, without demyelination, distinct from PML. Meningoencephalitis appears also to be a primary manifestation of SV40 infection and can be seen in conjunction with SV40-induced interstitial nephritis and pneumonitis. The difference in the lesions of meningoencephalitis and PML does not appear to be due to cellular tropism, as both oligodendrocytes and astrocytes are infected in PML and meningoencephalitis, as determined by in situ hybridization or immunohistochemistry for SV40 coupled with immunohistochemistry for cellular determinants. This is further supported by examination of SV40 nucleic acid sequences from the ori-enhancer and large-T-antigen regions, which reveals no tissue-or lesion-specific variation in SV40 sequences.  (+info)

Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. (5/4150)

Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.  (+info)

Increased ultraviolet sensitivity and chromosomal instability related to P53 function in the xeroderma pigmentosum variant. (6/4150)

The xeroderma pigmentosum (XP) variant (XPV) is a form of XP that has normal excision repair but shows defective DNA replication after UV irradiation. In developing various transformed fibroblast cell lines from these patients, we have found that there are significant phenotypic changes in transformed cells that seem to correlate with inactivation of p53. After transformation with SV40, XPV cell lines are only slightly UV sensitive, like their primary counterparts, but their sensitization with caffeine and the induction of sister chromatid exchanges (SCEs) by UV irradiation are greatly enhanced. After transformation by HPV16 E7, which targets the retinoblastoma cell cycle regulatory gene, there is no change in the UV sensitivity of XPV cells; but, when transformed by HPV16 E6 or E6 and E7 combined, there is a large increase in UV sensitivity and in the induction of SCEs. These changes are not associated with any detectable changes in the reactivation of an externally irradiated luciferase expression vector, the excision of cyclobutane pyrimidine dimers from bulk DNA, or unscheduled DNA synthesis and, therefore, do not involve excision repair. We suggest that if SCEs represent homologous recombination between sister chromatids, then in the absence of p53 function, the DNA chain arrest typical of UV-damaged XPV cells initiates strand exchange during recovery. In untransformed cells with normal p53, the preferred mode of recovery would then be replication bypass. The symptoms of elevated solar carcinogenesis in XPV patients may, therefore, be associated with increased genomic instability in cells of the skin in which p53 is inactivated by UV-induced mutations.  (+info)

Use of the Gal4-UAS technique for targeted gene expression in the zebrafish. (7/4150)

The most common way to analyze the function of cloned genes in zebrafish is to misexpress the gene product or an altered variant of it by mRNA injection. However, mRNA injection has several disadvantages. The GAL4-UAS system for targeted gene expression allows one to overcome some of these disadvantages. To test the GAL4-UAS system in zebrafish, we generated two different kinds of stable transgenic lines, carrying activator and effector constructs, respectively. In the activator lines the gene for the yeast transcriptional activator GAL4 is under the control of a given promoter, while in the effectors the gene of interest is fused to the sequence of the DNA-binding motif of GAL4 (UAS). Crosses of animals from the activator and effector lines show that effector genes are transcribed with the spatial pattern of the activators. This work smoothes the way for a novel method of misexpression of gene products in zebrafish in order to analyze the function of genes in developmental processes.  (+info)

The simian virus 40 small-t and large-T antigens jointly regulate cell cycle reentry in human fibroblasts. (8/4150)

Focus formation in human diploid fibroblasts (HDF cells) is known to require both the simian virus 40 (SV40) large-T and small-t antigens. Similarly, both SV40 proteins were required to stimulate confluent, density-arrested HDF cells to reenter the cell cycle. This study used defective recombinant adenoviruses to examine the roles of the individual SV40 proteins in altering specific steps in the cell cycle. Small-t antigen and, to a lesser extent, large-T antigen increased the level of the S phase cyclin cyclin A but without increasing the activity of associated cyclin kinases unless the two SV40 proteins were coexpressed. The absence of kinase activity reflected the presence in density-arrested cells of high levels of the cyclin-dependent kinase inhibitors p21(WAF1) and p27(KIP1). We report here that expression of SV40 large-T antigen reduced levels of p21(WAF1), while expression of small-t antigen was required to decrease p27(KIP1). The separate effects of large-T and small-t antigens on these two inhibitors may explain the joint requirement for the two proteins to drive cell cycle reentry of HDF cells and ultimately transform these cells.  (+info)

Simian virus (SV40) large T antigen, molecular model. This antigen is from the simian vacuolating virus 40 (SV40). Large T antigens play a role in regulating the viral life cycle of the polyomaviridae viruses, such as SV40. SV40 is found in monkeys such as Rhesus monkeys and macaques. Potentially tumour-causing in primates and humans, it is used in laboratory research and in vaccines. - Stock Image C025/1808
This topic contains 11 study abstracts on Simian virus 40 (SV40) indicating it may contribute to Simian virus 40 (SV40), Mesothelioma, and Cancer Metastasis
Simian virus 40 (SV40)-mediated transformation of human diploid fibroblasts has provided an effective experimental system for studies of both senescence in cell culture and carcinogenesis. Previous interpretations may have been complicated, however, by the semipermissive virus-cell interaction. In earlier studies, we previously demonstrated that the human diploid fibroblast line HS74 can be efficiently transformed by DNA from replication-defective mutants of SV40 containing a deletion in the viral origin for DNA synthesis (SVori-). In the current study, we found that such SVori- transformants show a significantly increased life span in culture, as compared with either HS74 or an independent transformant containing an intact viral genome, but they nonetheless undergo senescence. We have clonally isolated six immortalized derivatives of one such transformant (SV/HF-5). Growth studies indicate that the immortalized cell lines do not invariably grow better than SV/HF-5 or HS74. Genetic studies ...
In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent to the transforming activity lost by deletion of the E1A p300-binding region. T-antigen constructs from which the pRb-binding region has been deleted are virtually unable to induce foci of transformed cells in a ras cooperation assay in primary baby rat kidney cells. Nevertheless, such a construct can cooperate with an E1A N-terminal deletion mutant, itself devoid of transforming activity, to induce foci in this assay. The heterologous trans-cooperating activity observed between E1A and T-antigen deletion products is as efficient as trans cooperation between mutants expressing individual E1A domains. The cooperating function can be impaired by a deletion near the N terminus of T antigen. Such a deletion impairs neither the p53-binding function nor the activity of the pRb-binding region.. ...
The oligomers formed by a mutant nonkaryophilic large T antigen of simian virus 40, which lacks residues 110 through 152 of normal large T antigen and transforms only established cells (L. Fischer-Fantuzzi and C. Vesco, Proc. Natl. Acad. Sci. USA 82:1891-1895, 1985), were found to consist predominantly of dimers. Anti-p53 antibodies precipitated 14 to 16S complexes containing the mutant nonkaryophilic large T antigen and p53 from extracts of transformed cells, and anti-p53 indirect immunofluorescence stained these cells in the cytoplasm. ...
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The role of simian virus 40 (SV40) large tumor antigen (T antigen) as a DNA helicase at the replication fork was studied. We found that a T-antigen hexamer complex acts during the unidirectional unwinding of appropriate DNA substrates and is localized directly in the center of the fork, contacting the adjacent double strand as well as the emerging single strands. When bidirectional DNA unwinding, initiated at the viral origin of DNA replication, was analyzed, a larger T-antigen complex that is simultaneously active at both branch points of an unwinding bubble was observed. The size and shape of this helicase complex imply that the T-antigen dodecamer complex, assembled at the origin and active in the localized melting of duplex DNA, is subsequently also used to continue DNA unwinding bidirectionally. Then, however, the dodecamer complex does not split into two hexamer subunits that track along the DNA; rather, the DNA is threaded through the intact complex, with the concomitant extrusion of ...
You searched for: Creator Nathans, Daniel, 1928-1999 Remove constraint Creator: Nathans, Daniel, 1928-1999 Creator Lee, Theresa N. H. (Theresa N. H. Lee) Remove constraint Creator: Lee, Theresa N. H. (Theresa N. H. Lee) Subject DNA Cleavage Remove constraint Subject: DNA Cleavage Subject Simian virus 40 Remove constraint Subject: Simian virus 40 ...
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Deppert, W and Walter, G, Domains of simian virus 40 large t-antigen exposed on the cell surface. (1982). Subject Strain Bibliography 1982. 3601 ...
We previously analyzed human embryonic kidney (HEK) cell lines for the effects that simian virus 40 (SV40) small tumor antigen (ST) has on gene expression using Affymetrix U133 GeneChips. To cross-validate and extend our initial findings, we sought to compare the expression profiles of these cell lines using an alternative microarray platform. METHODS: We have analyzed matched cell lines with and without expression of SV40 ST using an Applied Biosystems (AB) microarray platform that uses single 60-mer oligonucleotides and single-color quantitative chemiluminescence for detection. RESULTS: While we were able to previously identify only 456 genes affected by ST with the Affymetrix platform, we identified 1927 individual genes with the AB platform. Additional technical replicates increased the number of identified genes to 3478 genes and confirmed the changes in 278 (61%) of our original set of 456 genes. Among the 3200 genes newly identified as affected by SV40 ST, we confirmed 20 by QRTPCR including
The genome of Simian Virus 40 (SV40) is a covalently closed circular DNA duplex with about 5200 nucleotide pairs. Based on physiological studies and mapping of temperature-sensitive and deletion...
We have analysed nucleotide sequences at the junction between simian virus 40 (SV40) and cellular DNA in the Fisher rat transformed line tsA30-N2. This line
simian virus 40, polio vaccines, and cancer I take no pleasure in these essays that expose the poor judgment and even recklessness of the US medical establishment. But, facts are facts, albeit unpleasant in many instances. (Update: Summer, 2014. We have recently heard that the introduction of anti-biotics in the early 1940s also seems to…
This entry was posted on June 2, 2011, 3:42 am and is filed under Fugitive Document. You can follow any responses to this entry through RSS 2.0. You can leave a response, or trackback from your own site. ...
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in Research in Veterinary Science (2009), 87(1), 123-32. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be ... [more ▼]. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be distinguished from simultaneously isolated colonocytes, as the jejunocytes specifically exhibited the small intestinal peptidase gene transcript, as well as an active alkaline phosphatase. The transformation of both types of cell suspension was performed by retroviral infection, using reproduction-defective viruses bearing the gene coding for the large T antigen of the leukaemia simian virus (SV40). The success of the transfection was demonstrated by (1) a significant increase in cell passage numbers (52-53 vs. 7 passages ...
4FGN: Analysis of the Costructure of the Simian Virus 40 T-Antigen Origin Binding Domain with Site I Reveals a Correlation between GAGGC Spacing and Spiral Assembly.
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TY - JOUR. T1 - Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription. AU - Nakagoshi, H.. AU - Nagase, T.. AU - Kanei-Ishii, C.. AU - Ueno, Y.. AU - Ishii, S.. PY - 1990/3/23. Y1 - 1990/3/23. N2 - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequene. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.. AB - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The ...
TY - JOUR. T1 - Transformation of DNA repair-deficient human diploid fibroblasts with a simian virus 40 plasmid. AU - Wood, C. M.. AU - Timme, T. L.. AU - Hurt, M. M.. AU - Brinkley, B. R.. AU - Ledbetter, D. H.. AU - Moses, R. E.. PY - 1987/4. Y1 - 1987/4. N2 - Fibroblasts from patients with xeroderma pigmentosum (XP) complementation groups A, C, D, E, and G, as well as Bloom syndrome (BS) and Fanconi anemia (FA) have been transfected with a plasmid, pSV7, containing the early region of Simian virus 40 (SV40). All of the cultures exhibited cytologic changes characteristic of transformed cells and expressed T-antigen. They also contained integrated copies of DNA derived from the vector, and in several cases, extrachromosomally replicated DNA. Not all of the transfected cultures became immortalized. The transformed xeroderma pigmentosum (XP) cultures retained their UV-sensitive phenotype in all but one case. The BS and FA cell lines retained their characteristic phenotype. All of the cultures, ...
DELETION OF THE CARBOXY TERMINUS OT SIMIAN VIRUS 40 LARGE T ANTIGEN AFFECTS VIRAL LATE GENE EXPRESSION A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy Terryl Stacy DARTMOUTH COLLEGE Hanover, New Hampshire March 8,1990 ...
Lambda-Tris(4,7-diphenyl-1,10-phenanthroline)cobalt(III), a photoactivated DNA-cleaving agent, is a small molecular probe of DNA structure. Because of its chirality, the complex cannot bind to regular right-handed B-form DNA but exhibits site-specific cleavage along the polymer strand at conformationally distinct sites such as those in a left-handed conformation. Both coarse and higher resolution mapping experiments using the chiral cobalt complex indicate intriguing conformational variations along the simian virus 40 genome. Highly specific cleavage is evident in the enhancer and promoter blocks and in the region downstream of 3 termini. A specific cleavage pattern borders an alternating purine/pyrimidine stretch within the enhancer, which was found earlier to bind anti-Z-DNA antibodies. Throughout the simian virus 40 genome, variations in structure delineated with the cobalt complex appear to correlate with regions important for control of gene expression. ...
Li J.J., Kelly T.J.. We recently described a soluble cell-free system derived from monkey cells that is capable of replicating exogenous plasmid DNA molecules containing the simian virus 40 (SV40) origin of replication (J.J. Li, and T.J. Kelly, Proc. Natl. Acad. Sci. U.S.A. 81:6973-6977, 1984). Replication in the system is completely dependent upon the addition of the SV40 large T antigen. In this report we describe additional properties of the in vitro replication reaction. Extracts prepared from cells of several nonsimian species were tested for the ability to support origin-dependent replication in the presence of T antigen. The activities of extracts derived from human cell lines HeLa and 293 were approximately the same as those of monkey cell extracts. Chinese hamster ovary cell extracts also supported SV40 DNA replication in vitro, but the extent of replication was approximately 1% of that observed with human or monkey cell extracts. No replication activity was detectable in extracts ...
Summary Simian virus 40 (SV40)-transformed cells express the SV40-specific tumour transplantation antigen (TSTA) on the cell surface and the SV40-coded tumour antigen in their nuclei. TSTA is defined by SV40-specific transplantation immunity, whereas T-antigen (T-Ag) can be detected serologically by indirect immunofluorescence. Both antigens, however, are derived from the A gene of SV40. We therefore analysed SV40-transformed cells for the presence of serologically detectable T-Ag-related molecules. Such antigens could not be detected on the surface of living SV40-transformed cells in monolayers. However, after a short formaldehyde fixation it was possible to stain the cell surfaces of SV40-transformed cells with sera from rabbits immunized with purified SDS-denatured T-Ag, but not with sera from hamsters bearing SV40-induced tumours. T-Ag-related antigens could be detected with both types of antisera by applying a more sensitive 125I-protein A assay. The T-Ag specificity of the binding of hamster SV40
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
The complete nucleotide sequence of the P gene of simian virus 41 (SV41) was determined. The gene was found to be 1406 nucleotides long and to contain a relatively small open reading frame encoding a cysteine-rich V protein with a calculated M r of 24076. We have demonstrated that RNA-editing events occur in SV41 P gene transcripts and that the ratio of edited mRNAs to faithfully copied mRNA (P-mRNA: V-mRNA) is about 1:5 at either 24 or 40 h post-infection. The mRNA with two G insertions was capable of encoding a P protein of 395 amino acids with a predicted M r of 41992. A kinetic study of P and V proteins by Western blot analysis showed that in virus-infected cells the amounts of both proteins were almost equal although the V-mRNA was considerably more abundant than the P-mRNA. Alignment of the SV41 P and V proteins with those of nine other paramyxoviruses demonstrated that irregular gaps were present around the RNA-editing sites.
By Catherine Frompovich After half a century, the 108th U.S. Congress House of Representatives Subcommittee on Human Rights and Wellness finally held a hearing September 10, 2003, on the simian virus (SV40) that was included in the original polio vaccine produced and administered to children in the 1950s and 1960s. Candidly, the first page of ...
SV40 and/or DNA sequences indistinguishable from SV40 have been detected in several types of human tumours. The oncoprotein of Simian virus 40, SV40 large T-antigen (Tag), is known to bind and inactivate tumour suppressor proteins, such as members of the retinoblastoma family and p53, thereby promot …
human alpha 1-antitrypsin promoter, human immunoglobulin heavy chain genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, Simian virus 40 poly A ...
Chang C, Simmons DT, Martin MA and Mora PT (1979) Identification and partial characterization of new antigens from simian virus 40-transformed mouse cells. J Virol 31: 463-471.. Crawford LV, Pim DC and Bulbrook RD (1982) Detection of antibodies against the cellular protein p53 in sera from patients with breast cancer. Int. J. Cancer 30: 403-408.. Crawford L (1983) The 53,000-dalton cellular protein and its role in transformation. Int. Rev. Exp. Path. 25: 1-50.. De Leo AB, Jay G, Appella E, Dubois GC, Law LW and Old LJ (1979) Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse. Proc Natl Acad Sci USA 76: 2420-2424.. Kress M, May E, Cassingena R and May P (1979) Simian Virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 serum. J. Virol. 31: 472-483.. Lane DP and Crawford LV (1979) T antigen is bound to a host protein in SV40-transformed cells. Nature 278: 261-263.. Linzer DIH and Levine AJ ...
Subramani, S. Rescue of chromosomal T-antigen sequences onto extrachromosomally replicating, defective simian virus 40 DNA by homologous recombination.. Molecular and Cellular Biology 6.4 (1986): 1320-1325. Web. 06 Aug. 2020. ...
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p53 is a cellular-encoded phosphoprotein first identified in protein complexes with the large tumor (T) antigen of simian virus 40 (SV40) (Linzer and Levine, 1979; Lane and Crawford, 1979). High...
A hybrid virus is a type of computer virus that combines elements of other virus programs in a new combination that will often...
Goat polyclonal antibody raised against synthetic peptide of V5 Tag. A synthetic peptide (conjugated with KLH) corresponding to amino acids 95-108 of RNA polymerase alpha of subunit simian virus 5. (PAB14287) - Products - Abnova
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A comparison of the proteins synthesized in human cells at late times after infection with adenovirus (Ad2) and with the adeno-simian virus 40 (SV40) hybrid viruses revealed polypeptides of 30,000 and 92,000 molecular weight specific for the hybrid viruses Ad2+ND1 and Ad2+ND4, respectively. Cell-free translation of SV40-specific mRNA, prepared from these cells by hybridization of total cytoplasmic RNA to SV40 DNA, showed that the mRNAs specifying these two polypeptides were at least partially encoded by the SV40 portion of the hybrid viruses. Cell-free translation of SV40-specific mRNA prepared from monkey cells infected with SV40 produced polypeptides of 40,000, 43,000, 48,500, and 92,000 molecular weight. The SV40 and Ad2+ND4 92,000-molecular-weight polypeptides made in vitro were very similar in electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels to the polypeptide precipitated by Tegtmeyer (1974) with SV40 anti-T serum.
Human polyomaviruses (JC virus, BK virus and simian virus 40) are causative agents of some human diseases and, interestingly, are involved in processes of cell transformation and oncogenesis. These viruses need the cell cycle machinery of the host cell to complete their replication; so they evolved mechanisms that can interfere with the growth control of infected cells and force them into DNA replication. The retinoblastoma family of proteins (pRb), which includes pRb/p105, p107 and pRb2/p130, acts as one of the most important regulators of the G1/S transition of the cell cycle. Rb proteins represent an important target for viral oncoproteins. Early viral T antigens can bind all members of the pRb family, promoting the activation of the E2F family of transcription factors, thus inducing the expression of genes required for the entry to the S phase. The interaction between early viral antigens and cell cycle regulators represents an important mechanism through which viruses deregulate cell cycle ...
Transport of α-aminoisobutyric acid and 2-deoxy-d-glucose in African green monkey kidney cells was measured 8 to 100 hr following permissive simian virus 40 infection. No differences in transport were detected during the time-period studies, and no significant differences were seen between the apparent Michaelis-Menten constants of normal and virally infected cells. The absence of transport enhancement in permissive simian virus 40 infection suggests that the augmented transport of viral-transformed cell lines devolves upon altered host genome function.. ...
Alpha-globin transcription factor CP2 is a protein that in humans is encoded by the TFCP2 gene.[1][2] TFCP2 is also called Late SV40 factor (LSF) and it is induced by well known oncogene AEG-1.[3] Late SV40 factor (LSF) also acts as an oncogene in hepatocellular carcinoma.[4] Late SV40 factor (LSF) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9).[5] Along with its main oncogene function in hepatocellular carcinoma (HCC) it plays multifaceted role in chemoresistance, epithelial-mesenchymal transition (EMT), allergic response, inflammation and Alzheimers disease.[4][6] The small molecule FQI1 (factor quinolinone inhibitor 1) prevents LSF from binding to HCC DNA which results in HCC cell death.[4][6][7] ...
COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication. The CV-1 cell line in turn was derived from the kidney of the African green monkey. The acronym COS is derived from the cells being CV-1 (simian) in Origin, and carrying the SV40 genetic material. Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell lines are often used by biologists when studying the monkey virus SV40. Cells from these lines are also often transfected to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. When an expression construct with an SV40 promoter is introduced into COS cells, the vector can be replicated substantially by the large T antigen. These COS cells are genetically modified to produce the T antigen from their own genome. Jensen FC, Girardi AJ, Gilden RV, Koprowski H ...
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Because of the high homology that exists between the DNA of SV40 and the DNA of the JC or BK viruses, the identity of the band obtained by PCR and visualized by
A family of dihydroquinolinones that inhibited the proliferation of a number of cancer cell lines and targeted the oncogenic activities of the late simian virus 40 factor (LSF) was discovered. The lead quinolinone inhibitors, 8-(2-propoxyphenyl)-7,8-dihydro-[1,3]dioxolo[4,5-g]quinolin-6(5H)-one, FQI1, and 8-(2-propoxyphenyl)-[1,3]dioxolo[4,5-g]quinolin-6(5H)-one, FQI2, were determined by a comprehensive SAR study. The lead compounds had low micromolar to nanomolar Gi50S and IC50S (concentrations that induced 50% inhibition) in cell growth and LSF-directed luciferase reporter assays, respectively. A distinct correlation between the GI50 and IC50 values indicated antiproliferative effects resulted from inhibition of LSF activity. FQI1 had no growth effects on immortalized human hepatocytes or primary mouse hepatocytes. Overall, FQI1 proved a good drug candidate for hepatocellular carcinoma (HCC). It possessed a low molecular weight and moderate solubility, which was improved by substitution of the ...
mouse connexin45(Cx45) genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), mouse phosphoglycerate kinase promoter (PGK promoter), Phage P1 loxP, E. coli neo, E. coli ...
Simian Virus 40 or Simian vacuolating virus 40 - a polyomavirus that is found in both monkeys and humans. As with other polyomaviruses, it is a DNA virus that can cause tumors. ...
washed sheep red bloodand, hereby, warrant that these ingredients are safe for injection into the body of my patient. I have researched reports to the contrary, such as reports that mercury thimerosal causes severe neurological and immunological damage, and find that they are not credible.I am aware that some vaccines have been found to have been contaminated with Simian Virus 40 (SV 40) and that SV 40 is causally linked by some researchers to non-Hodgkins lymphoma and mesotheliomas in humans as well as in experimental animals. I hereby warrant that the vaccines I employ in my practice do not contain SV 40 or any other live viruses. (Alternately, I hereby warrant that said SV-40 virus or other viruses pose no substantive risk to my patient.)I hereby warrant that the vaccines I am recommending for the care of (Patients name) _______________ do not contain any tissue from aborted human babies (also known as fetuses ...
Ongoing and late side effects are related to the treatment you had and its effectiveness, your type and stage of cancer and your overall health after treatment.
Distributor of Immunohistochemistry IHC antibodies, Flow Cytometry, Molecular Biology, Staining Kits, Reagents, Detection Systems Ancillary reagents
Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 0.1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 0.1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Anderson HA, Chen Y, Norkin LC. 1998. MHC class I molecules are enriched in caveolae but do not enter with simian virus 40.. J Gen Virol. 79 ( Pt 6):1469-77. ...
Nuclear targeting sequences are essential for the transport of proteins into the nucleus. The seven-amino-acid nuclear targeting sequence of the SV40 large T antigen has been regarded as the model; however, many nuclear targeting sequences appear to be more complex. We suggest in this review that, d …
include ,EXTERN.h, #include ,perl.h, static PerlInterpreter *my_perl; /** my_eval_sv(code, error_check) ** kinda like eval_sv(), ** but we pop the return value off the stack **/ SV* my_eval_sv(SV *sv, I32 croak_on_error) { dSP; SV* retval; PUSHMARK(SP); eval_sv(sv, G_SCALAR); SPAGAIN; retval = POPs; PUTBACK; if (croak_on_error && SvTRUE(ERRSV)) croak(SvPVx_nolen(ERRSV)); return retval; } /** match(string, pattern) ** ** Used for matches in a scalar context. ** ** Returns 1 if the match was successful; 0 otherwise. **/ I32 match(SV *string, char *pattern) { SV *command = newSV(0), *retval; sv_setpvf(command, my $string = %s; $string =~ %s, SvPV_nolen(string), pattern); retval = my_eval_sv(command, TRUE); SvREFCNT_dec(command); return SvIV(retval); } /** substitute(string, pattern) ** ** Used for =~ operations that modify their left-hand side (s/// and tr///) ** ** Returns the number of successful matches, and ** modifies the input string if there were any. **/ I32 substitute(SV **string, char ...
include ,EXTERN.h, #include ,perl.h, static PerlInterpreter *my_perl; /** my_eval_sv(code, error_check) ** kinda like eval_sv(), ** but we pop the return value off the stack **/ SV* my_eval_sv(SV *sv, I32 croak_on_error) { dSP; SV* retval; STRLEN n_a; PUSHMARK(SP); eval_sv(sv, G_SCALAR); SPAGAIN; retval = POPs; PUTBACK; if (croak_on_error && SvTRUE(ERRSV)) croak(SvPVx(ERRSV, n_a)); return retval; } /** match(string, pattern) ** ** Used for matches in a scalar context. ** ** Returns 1 if the match was successful; 0 otherwise. **/ I32 match(SV *string, char *pattern) { SV *command = NEWSV(1099, 0), *retval; STRLEN n_a; sv_setpvf(command, my $string = %s; $string =~ %s, SvPV(string,n_a), pattern); retval = my_eval_sv(command, TRUE); SvREFCNT_dec(command); return SvIV(retval); } /** substitute(string, pattern) ** ** Used for =~ operations that modify their left-hand side (s/// and tr///) ** ** Returns the number of successful matches, and ** modifies the input string if there were any. **/ I32 ...
This module is the first in a series that discusses the discovery, structure, and function of DNA. Key experiments are discussed: from Griffiths discovery of genetic
Ive been reading a lot about CFIDs and fibro. Some theories are that is has a viral origin, EBV and HHV6 specifically.I have tested postive for both these viruses.....
Latest Side Order of Life review: I have just started watching Side order of life and I loved it. Although I saw just a couple of ...
Simian vacuolating virus 40, known as SV40, is a virus found in monkeys and humans, which has the potential to cause cancer, in ... Martini, F; Corallini, A; Balatti, V; Sabbioni, S; Pancaldi, C; Tognon, M (9 July 2007). "Simian virus 40 in humans". ... NIH/National Cancer Institute (2004-08-25). "Studies Find No Evidence That Simian Virus 40 Is Related To Human Cancer". Science ... Hilleman MR (1998). "Discovery of simian virus 40 (SV40) and its relationship to poliomyelitis virus vaccines". Dev Biol Stand ...
Mertz, Janet E. (1975). DELETION MUTANTS OF SIMIAN VIRUS 40 (PhD). Stanford University. Mertz, Janet E. (1975). DELETION ... Biochemical method for inserting new genetic information into DNA of simian virus 40. Proc Natl Acad Sci USA 69:2904-2909. ... MUTANTS OF SIMIAN VIRUS 40. (PhD). Stanford University.. "Janet Mertz's curriculum vitae, 1984". CSHL Archives Repository. ... Her laboratory studies regulation of expression of the genes of the DNA oncoviruses SV40, hepatitis B virus, and Epstein-Barr ...
"Simian Virus 40 (SV40), Polio Vaccine, and Cancer". Vaccine Safety. Centers for Disease Control. 22 April 2004. Archived from ... which helps prevent infection with wild virus in areas where the virus is endemic. The live virus used in the vaccine can ... Vilchez RA, Kozinetz CA, Arrington AS, Madden CR, Butel JS (June 2003). "Simian virus 40 in human cancers". The American ... Carbone M (December 1999). "Simian virus 40 and human tumors: It is time to study mechanisms". Journal of Cellular Biochemistry ...
Maxwell, SA; Ames, SK; Sawai, ET; Decker, GL; Cook, RG; Butel, JS (February 1991). "Simian virus 40 large T antigen and p53 are ... Vilchez, RA; Kozinetz, CA; Arrington, AS; Madden, CR; Butel, JS (1 June 2003). "Simian virus 40 in human cancers". The American ... Vilchez, RA; Butel, JS (September 2003). "Simian virus 40 and its association with human lymphomas". Current Oncology Reports. ... "Influence of the viral regulatory region on tumor induction by simian virus 40 in hamsters". Journal of Virology. 82 (2): 871-9 ...
Vilchez RA, Kozinetz CA, Arrington AS, Madden CR, Butel JS (June 2003). "Simian virus 40 in human cancers". The American ... Glioblastoma has been associated with the viruses SV40, HHV-6, and cytomegalovirus. Research has been done to see if ... 14 (1): 40-51. doi:10.1111/ajco.12756. PMID 28840962. Kuehn BM (March 2010). "Genomics illuminates a deadly brain cancer". JAMA ... Historically, around 90% of patients with glioblastoma underwent anticonvulsant treatment, although only an estimated 40% of ...
Aloni Y, Dhar R, Khoury G (October 1979). "Methylation of nuclear simian virus 40 RNAs". Journal of Virology. 32 (1): 52-60. ... Many RNA viruses including SV40, adenovirus, herpes virus, Rous sarcoma virus, and influenza virus have been known to contain ... and Zika virus (ZIKV). These results suggest m6A and its cognate factors play crucial roles in regulating virus life cycles and ... It is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, ...
"BK Virus, JC Virus and Simian Virus 40 Infection in Humans, and Association with Human Tumors". Polyomaviruses and Human ... "The relationship between simian virus 40 and mesothelioma". Current Opinion in Pulmonary Medicine. 14 (4): 316-21. doi:10.1097/ ... The virus causing this illness was isolated in 1937. The rash typical of Lyme borreliosis was identified the early 1900s. ... Natelson, BH; Ye, N; Moul, DE; Jenkins, FJ; Oren, DA; Tapp, WN; Cheng, YC (1994). "High titers of anti-Epstein-Barr virus DNA ...
"Monoclonal antibodies specific for simian virus 40 tumor antigens." Journal of Virology 39.3 (1981): 861-869. Tsai, Li-Huei, et ... Harlow, E (1981). "Monoclonal antibodies specific for simian virus 40 tumor antigens". J Virol. 39: 861-9. PMC 171319. PMID ... "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product." Science 243.4893 (1989): 934- ... Dyson, N (1989). "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product". Science. 243 ...
A late protein is a viral protein that is formed after replication of the virus. One example is VP4 from simian virus 40 (SV40 ... Early protein "DNA Virus Replication". Daniels R, Sadowicz D, Hebert DN (July 2007). "A very late viral protein triggers the ... "Organization of the major and minor capsid proteins in human papillomavirus type 33 virus-like particles". J. Gen. Virol. 76 (9 ...
Hamer, D.H.: Simian Virus 40 as a cloning vehicle in mammalian cells. In Schultz, Jr. and Brada, Z. (Eds.): Genetic ... Dean Hamer was born in Montclair, N.J." Hamer D.H.; Davoli D.; Thomas C.A., Jr; Fareed G.C. (1977). "Simian virus 40 carrying ... As a means to reduce the latent pools of virus responsible for viral persistence, they discovered novel chemical agent to ... "Rational design of drugs that induce human immunodeficiency virus replication". Journal of Virology. 77 (19): 10227-36. doi: ...
Turk, B; Porras, A; Mumby, MC; Rundell, K (June 1993). "Simian virus 40 small-t antigen binds two zinc ions". Journal of ... Cheng, Jingwei; DeCaprio, James A.; Fluck, Michele M.; Schaffhausen, Brian S. (2009). "Cellular transformation by Simian Virus ... "Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria". Journal of Virology ... In SV40 and JC virus, STag is not required for viral proliferation, but does improve efficiency. In SV40, STag has a similar ...
"SSA/Ro antigen expression in simian virus 40-transformed human keratinocytes". The Journal of Investigative Dermatology. 90 (3 ...
Effect of 5-Azacytidine on simian virus 40 DNA replication (Thesis). OCLC 6039668. "Marian Johnson-Thompson, Ph.D." www. ...
14 species are known to infect humans, while others, such as Simian Virus 40, have been identified in humans to a lesser extent ... most notably BK virus, JC virus, and SV40. It is essential for proliferation in the viruses that express it and is thought to ... Kelley WL, Georgopoulos C (April 1997). "The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can ... Two viruses-HPyV6 and HPyV7-are most closely related to KI and WU viruses, while HPyV9 is most closely related to the African ...
"Cellular transformation by Simian Virus 40 and Murine Polyoma Virus T antigens". Seminars in Cancer Biology. 19 (4): 218-228. ... "Polyoma virus middle T is essential for virus replication and persistence as well as for tumor induction in mice". Virology. ... However, evidence of MTag encoding and expression has also recently been reported in at least one virus of unrelated lineage, ... Courtneidge, Sara A.; Smith, Alan E. (2 June 1983). "Polyoma virus transforming protein associates with the product of the c- ...
Cheng, Jingwei; DeCaprio, James A.; Fluck, Michele M.; Schaffhausen, Brian S. (2009). "Cellular transformation by Simian Virus ... although the exact molecular mechanisms vary from one virus to another. The SV40 large T antigen from the SV40 virus is the ... LTag has two primary functions, both related to replication of the viral genome: it unwinds the virus's DNA to prepare it for ... Some polyomavirus LTag proteins - most notably the well-studied SV40 large tumor antigen from the SV40 virus - are oncoproteins ...
Other risk factors include genetics and infection with the simian virus 40. The diagnosis may be suspected based on chest X-ray ... Some studies suggest that simian virus 40 (SV40) may act as a cofactor in the development of mesothelioma. This has been ... Asbestos exposure and the onset of cancer are generally separated by about 40 years. Washing the clothing of someone who worked ... It is virtually never less than fifteen years and peaks at 30-40 years. The duration of exposure to asbestos causing ...
"Biochemical Procedure for Production of Small Deletions in Simian Virus 40 DNA." Proccedings of the National Academy of ...
Paul D, Lipton A, Klinger I (1971). "Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts ...
Later, Daniel Nathans and Kathleen Danna showed that cleavage of simian virus 40 (SV40) DNA by restriction enzymes yields ... Danna K, Nathans D (December 1971). "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus ... There is research on REases and ZFN that can cleave the DNA of various human viruses, including HSV-2, high-risk HPVs and HIV-1 ... The term restriction enzyme originated from the studies of phage λ, a virus that infects bacteria, and the phenomenon of host- ...
"Interactions of early mouse embryos with oncogenic viruses-Simian virus 40 and polyoma. I. Ultrastructural studies." Journal ... "Virus particles in early mouse embryos." Journal National Cancer Institute 51 (1973): 1041-1050. Print. Lewandowski, L.J., F.S ... "Analysis of a viral agent isolated from multiple sclerosis (MS) brain tissue: Characterization as a parainfluenza virus type I ... They were cultured." At Wistar, Pienkowski collaborated with Koprowski on research into cancer-causing viruses. Upon returning ...
... 'SV40 stands for Simian Virus 40' official website website in memory of Alexander Horwin. ... Rogue virus in the vaccine: Early polio vaccine harbored virus now feared to cause cancer in humans, William Carlsen, San ... Martin's Press, 2004, ISBN 0-312-27872-1 The Virus and the Vaccine official website "The Virus and the Vaccine", Debbie ... The Virus and the Vaccine, The Reading Room, WNYC website. Who We Are, SV40 Cancer Foundation official website Demanding a ...
Gruss P, Dhar R, Khoury G (February 1981). "Simian virus 40 tandem repeated sequences as an element of the early promoter". ... 159 (6): 1327-40. doi:10.1016/j.cell.2014.11.023. PMC 4364385. PMID 25480297. Sun J, Rockowitz S, Xie Q, Ashery-Padan R, Zheng ...
Stillman BW, Gluzman Y (August 1985). "Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells". ... Viruses offer an efficient means of delivering genes into cells, which they evolved to do, and are thus of great use as ... This danger can be avoided by the use of viruses which lack key genes, and which are thus unable to replicate after entering a ... They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy. HEK 293 cells ...
BK virus or Simian virus. The Ebola virus may also be found in urine from an infected person. The exact survival time of this ... Vanchiere, John A. (12 January 2005). "Detection of BK virus and simian virus 40 in the urine of healthy children". Journal of ... "Ebola (Ebola Virus Disease)Transmission, Q&As on Transmission". CDC (Centres for Disease Control and Prevention). 20 November ... particular virus in human urine outside of the human body is unclear but probably "up to several days" like with other body ...
Yamamoto H, Shimojo H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ... Once the virus has successfully gained entry into the host cell, the endosome acidifies, which alters virus topology by causing ... Although this is significantly larger than other viruses in its Baltimore group, it is still a very simple virus and is heavily ... Medicine portal Viruses portal VA (viral associated) RNA "9.11H: Double-Stranded DNA Viruses- Adenoviruses". Biology LibreTexts ...
"Spliced and unspliced virus specific RNA sequences are associated with purified simian virus 40 chromatin". Nucleic Acids ... "Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts". Nature. 323 (6088): 544-8. doi: ... Section DNA Tumor Viruses (Gerhard Sauer) for doctoral studies. He received his PhD from Ruprecht-Karls-University, Heidelberg ...
"Identification of the oncogenic substance in rhesus monkey kidney cell culture as simian virus 40". Virology. 17 (1): 65-75. ... The virus was named the Stewart-Eddy or SE polyoma virus, after their respective surnames. Their collaborative efforts earned ... the discovery explained the origins of the widespread contamination of a variety of stocks of seed viruses and live polio virus ... In parallel to her job as chief of flu virus vaccine testing Eddy began research on polio vaccines at NIH in 1952. In 1953 she ...
Nakanishi A, Itoh N, Li PP, Handa H, Liddington RC, Kasamatsu H (April 2007). "Minor capsid proteins of simian virus 40 are ... April 2006). "The VP2/VP3 minor capsid protein of simian virus 40 promotes the in vitro assembly of the major capsid protein ... Clever J, Dean DA, Kasamatsu H (October 1993). "Identification of a DNA binding domain in simian virus 40 capsid proteins Vp2 ... Gasparovic ML, Gee GV, Atwood WJ (November 2006). "JC virus minor capsid proteins Vp2 and Vp3 are essential for virus ...
... (Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from ... Chen S, Paucha E (July 1990). "Identification of a region of simian virus 40 large T antigen required for cell transformation ... Archived 2004-01-19 at the Wayback Machine Sweet BH, Hilleman MR (November 1960). "The vacuolating virus, S.V. 40". Proc. Soc. ... Protein-protein interactions between T-antigen and DNA polymerase-alpha directly stimulate replication of the virus genome. T- ...
This lab outbreak was initially diagnosed as simian haemorrhagic fever virus (SHFV) and occurred amongst a shipment of crab- ... The four are Bundibugyo virus (BDBV), Sudan virus (SUDV), Taï Forest virus (TAFV) and one simply called Ebola virus (EBOV, ... The virus responsible for the initial outbreak, first thought to be Marburg virus, was later identified as a new type of virus ... Main articles: Ebola virus cases in the United States, Ebola virus disease in Spain, and Ebola virus disease in the United ...
"Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing ... 40] en 1987, D. T. Burke et al. describen o uso de cromosomas artificiais de lévedo (YAC, Yeast Artificial Chromosome),[41] e ...
"Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". ... Molekul DNA rekombinan pertama dibuat oleh Paul Berg pada tahun 1972 dengan menggabungkan DNA virus monyet SV40 dengan virus ... "Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing ... Paul Berg menciptakan molekul DNA rekombinan pertama dengan menggabungkan DNA dari virus monyet SV40 dengan virus lambda.[22] ...
... passage of simian immunodeficiency virus by unsterile injections and the emergence of epidemic human immunodeficiency virus in ... O grupo de Montagnier denominou o seu novo virus illado virus asociado á linfadenopatía (LAV).[181] Como estes dous virus se ... Despois de que o virus entra no corpo hai un período de rápida replicación viral, que fai que o virus sexa abondoso no sangue ... O grupo de Gallo chamou o seu novo virus illado HTLV-III. Ao mesmo tempo, o grupo de Montagnier illou un virus dun paciente que ...
It is a member of the Semliki Forest virus complex and is closely related to Ross River virus, O'nyong'nyong virus, and Semliki ... and false positives can occur with infection due to other related viruses, such as o'nyong'nyong virus and Semliki Forest virus ... Chikungunya virus is passed to humans when a bite from an infected mosquito breaks the skin and introduces the virus into the ... This mutation potentially allows the virus to multiply more easily in mosquito cells.[74] The change allows the virus to use ...
Carbone M (1999). "Simian virus 40 and human tumors: It is time to study mechanisms". J Cell Biochem. 76 (2): 189-93. PMID ... "Simian Virus 40 (SV40), Polio Vaccine, and Cancer". Vaccine Safety. Centers for Disease Control. 2004-04-22. Retrieved 2010-12- ... Vilchez R, Kozinetz C, Arrington A, Madden C, Butel J (2003). "Simian virus 40 in human cancers". Am J Med. 114 (8): 675-84. ... "Cancer risk associated with receipt of vaccines contaminated with simian virus 40: epidemiologic research" (PDF). Expert Rev ...
Jackson, D. A., Symons, R. H., Berg, P. Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: ... Jaenisch, R., Mintz, B. Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts ... New virus-built battery could power cars, electronic devices [online]. Web.mit.edu, 2009-04-02 [cit. 2010-07-17]. Dostupné ... Hidden Ingredient In New, Greener Battery: A Virus [online]. Npr.org [cit. 2010-07-17]. Dostupné online. (anglicky). Je zde ...
... and Simian virus 40 in 1976 and 1978, respectively.[14][15] ... Viruses and bacteriophagesEdit. Bacteriophages have played and ... "Complete genomes: Viruses". NCBI. 2011-11-17. Retrieved 2011-11-18.. *^ "Genome Project Statistics". Entrez Genome Project. ... 2,719 viruses, 1,115 archaea and bacteria, and 36 eukaryotes, of which about half are fungi.[29][30] ... While the growth in the use of the term has led some scientists (Jonathan Eisen, among others[40]) to claim that it has been ...
Jackson, RJ; DJ Maguire; LA Hinds; IA Ramshaw (1998). "Infertility in mice induced by a recombinant ectromelia virus expressing ... development of a simian model for testing of anti-FA-1 contraceptive vaccine". J Reprod Immunol. 27: 111-121. doi:10.1016/0165- ... Research in these countries has therefore focused on genetically modifying viruses or microorganisms that infect the unwanted ... in Australia by engineering rabbit zona pellucida glycoproteins into a recombinant myxoma virus. This approach has induced ...
... virus called the "human respiratory virus-27". The study used a fake virus to prevent participants from having thoughts, ... The resulting choices of the human and simian subjects concorded with the theory of cognitive dissonance when the children and ... The study participants were in two groups; one group was told that they were actual candidates for the virus-27 test, and the ... opinions, and feeling about the virus that would interfere with the experiment. ...
1972). Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules ...
Famili Polyomaviridae - termasuk Simian virus 40, virus JC. *Famili Poxviridae - termasuk Cowpox virus, smallpox ... Virus DNA adalah virus yang masuk pada baik Grup I atau Grup II pada sistem klasifikasi Baltimore terhadap virus. Virus ini ... "ICTVdb Index of Viruses: Virus Taxonomy, 8th Reports of the International Committee on Taxonomy of Viruses: Listing in ... Artikel bertopik virus ini adalah sebuah rintisan. Anda dapat membantu Wikipedia dengan mengembangkannya.. ...
"Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing ... biokütus - vähendatakse biokütuse tootmiskulusid.[40]. Geenmuundatud kultuurid[muuda , muuda lähteteksti]. GM-kultuuride ... Atlandi lõhe 40 000 geenist lisati harilikult Chinooki lõhes kasvav hormoonide regulatoorne geen ja Zoarces americanus'e ... "Development of genetically engineered resistant papaya for papaya ringspot virus in a timely manner: a comprehensive and ...
"Expression of enhanced levels of small RNA polymerase III transcripts encoded by the B2 repeats in simian virus 40-transformed ... 40 (2): 158-60. doi:10.1038/ng.2007.55. PMC 2647775 . PMID 18176564. Pibouin L, Villaudy J, Ferbus D, et al. (February 2002). " ... One ncRNA, termed HOTAIR, that originates from the HOXC locus represses transcription across 40 kb of the HOXD locus by ... tiling array data shows more than 40% of transcripts are non-polyadenylated). However, unambiguously identifying ncRNAs within ...
"Specific Cleavage of Simian Virus 40 DNA by Restriction Endonuclease of Hemophilus Influenzae". Proc. Natl. Acad. Sci. U.S.A. ... StuI[40][41]. Streptomyces tubercidicus 5'AGGCCT 3'TCCGGA 5'---AGG CCT---3' 3'---TCC GGA---5' ...
Ang mga ito ang henetikong impormasyon na nakokodigo sa asidong nukleyiko(DNA, o RNA para sa mga virus), naka-transkriba sa RNA ... Ang natitirang Haplorhini na Simian ay naghiwalay sa mga pangkat na Catarrhini at Platyrrhini. Ang linyang Platyrrihni(Bagong ... Ang mga Endogenous retrovirus (ERV) ang mga labing sekwensiya ng genome na naiwan mula sa mga sinaunang impeksiyong pang-virus ... Ang mga Virus ay nagdadala rin ng DNA sa pagitan ng mga organismo na pumapayag sa paglilipat ng mga gene sa ibayong mga dominyo ...
Another metallo-porphyrin complex, the iron chelate, is more photoactive (towards HIV and simian immunodeficiency virus in MT-4 ... fungi and viruses.[1] PDT is popularly used in treating acne. It is used clinically to treat a wide range of medical conditions ... Photosensitisers have been employed to sterilise blood plasma and water in order to remove blood-borne viruses and microbes and ... In the 1980s, David Dolphin, Julia Levy and colleagues developed a novel photosensitizer, verteporfin.[39][40] Verteporfin, a ...
Populations Exposed To Simian Immunodeficiency Viruses". ScienceDaily. Retrieved 2009-08-15.. ... extinct non-hominoid simians, including early Catarrhines are discussed as monkeys as well as simians or anthropoids,[32][33][ ... Infraorder Simiiformes: simians *Parvorder Platyrrhini: New World monkeys *Family Callitrichidae: marmosets and tamarins (42 ... Extinct basal simians such as Aegyptopithecus or Parapithecus [35-32 million years ago], eosimiidea and sometimes even the ...
"Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". ... "Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing ... Paul Berg created the first recombinant DNA molecules by combining DNA from the monkey virus SV40 with that of the lambda virus ... Fungal and virus resistant crops have also been developed or are in development.[125][126] This makes the insect and weed ...
Multiplicity reactivation has been found to occur with pathogenic viruses including influenza virus, HIV-1, adenovirus simian ... Viruses[edit]. Viruses are capsid-encoding organisms composed of proteins and nucleic acids that can self-assemble after ... Many types of virus are capable of genetic recombination. When two or more individual viruses of the same type infect a cell, ... When two or more viruses, each containing lethal genomic damage infect the same host cell, the virus genomes often can pair ...
"Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing ... As of 2005, about 13% of the Zucchini (a form of squash) grown in the US was genetically modified to resist three viruses; that ... Papaya was genetically modified to resist the ringspot virus (PSRV). "SunUp" is a transgenic red-fleshed Sunset papaya cultivar ... Its single-handed savior was a breed engineered to be resistant to the virus. Without it, the state's papaya industry would ...
2005). "Central African hunters exposed to simian immunodeficiency virus". Emerg Infect Dis 11 (12): 1928-30. doi:10.3201/ ... passage of simian immunodeficiency virus by unsterile injections and the emergence of epidemic human immunodeficiency virus in ... 37.0 37.1 Reeves, J. D. and Doms, R. W (2002). "Human Immunodeficiency Virus Type 2". J. Gen. Virol. 83 (Pt 6): 1253-65. doi: ... Laurence J (2006). "Hepatitis A and B virus immunization in HIV-infected persons". AIDS Reader 16 (1): 15-17. PMID 16433468 . ...
Sato, S (February 1978). "A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus ...
Ang malapit na kaugnay na simian immunodeficiency virus (SIV) ay nag-ebolve sa maraming mga strain na inuri sa mga natural na ... VIRUSES , F. ANIMAL VIRUS LIFE CYCLES , 3. The Life Cycle of HIV Community College of Baltimore County. Updated: Jan., 2008 ... "Development of vivo of genetic variability of simian immunodeficiency virus". Proc. Natl. Acad. Sci. U.S.A. 88 (18): 8126-30. ... ang virus ay maaaring maging latento na pumapayag sa virus at mga selulang hosto nito na makaiwas sa pagkakatuklas (detection) ...
Epstein-Barr virus (EBV) infection is associated with nasopharyngeal cancer. Nasopharyngeal cancer occurs endemically in some ... and 8-oxo-purines in simian kidney cells". Nucleic Acids Res. 34 (8): 2305-15. doi:10.1093/nar/gkl099. PMC 1458282 . PMID ... Distinct host genome methylation and expression patterns, produced even when virus isn't integrated into the host genome. ... and Epstein-Barr virus. Head and neck cancers are most commonly of the squamous cell carcinoma type. The diagnosis is confirmed ...
"Expression cloning of new receptors used by simian and human immunodeficiency viruses.". Nature 388 (6639): 296-300. PMID ... 2003). "Expression of chemokines and their receptors in human and simian astrocytes: evidence for a central role of TNF alpha ... orphan seven-transmembrane segment receptors which are expressed in CD4-positive cells support simian immunodeficiency virus ... "Gp120-induced Bob/GPR15 activation: a possible cause of human immunodeficiency virus enteropathy.". Am. J. Pathol. 159 (5): ...
... bunchy top virus. Banana bunchy top virus (BBTV) is a plant virus of the genus Babuvirus, family Nanonviridae affecting ... "Comparing Black People to Monkeys has a Long, Dark Simian History". Huffington Post. Archived from the original on January 3, ... Infected plants may produce no fruit or the bunch may not emerge from the pseudostem.[90] The virus is transmitted by the ... There is no cure for BBTD, but it can be effectively controlled by the eradication of diseased plants and the use of virus-free ...
The genome of Simian Virus 40 (SV40) is a covalently closed circular DNA duplex with about 5200 nucleotide pairs. Based on ... The genome of Simian Virus 40 (SV40) is a covalently closed circular DNA duplex with about 5200 nucleotide pairs. Based on ... Nathans D., Gutai M.W. (1978) DNA Recombination in Cells Infected with Simian Virus 40. In: Hofschneider P.H., Starlinger P. ( ...
7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated ... Simian virus 12 (strain wt100) (SV-12) (Baboon polyomavirus 1). Simian virus 12. Cercopithecus erythrotis polyomavirus 1. And ... Simian virus 12 (strain wt100) (SV-12) (Baboon polyomavirus 1). Simian virus 12. Cercopithecus erythrotis polyomavirus 1. And ... "The structure of simian virus 40 refined at 3.1-A resolution.". Stehle T., Gamblin S.J., Yan Y., Harrison S.C.. Structure 4:165 ...
The simian virus 40 late polyadenylation signal (SVLPA) is an RNA element which promotes efficient polyadenylation. Wu, C.; ... Page for Simian virus 40 late polyadenylation signal (SVLPA) at Rfam v t e. ...
... indicating it may contribute to Simian virus 40 (SV40), Mesothelioma, and Cancer Metastasis ... This topic contains 11 study abstracts on Simian virus 40 (SV40) ... Serum antibodies to JC virus, BK virus, simian virus 40, and ... Vertically transmitted viral infection and polyviral infection with BK virus, JC virus and SV40 virus are significantly more ... SV40 is an abbreviation for Simian vacuolating virus 40 or Simian virus 40, a polyomavirus that is found in both monkeys and ...
1996) Simian virus 40 large T antigen (SV40LTAg) primer specific DNA amplification in human pleural mesothelioma tissue. Thorax ... 1999) Cell and molecular biology of Simian virus 40: implications for human infections and disease. J Natl Cancer Inst 91:119- ... 1998) Contamination of poliovirus vaccines with simian virus 40 (1955-1963) and subsequent cancer rates. JAMA 279:292-295, . ... Mulatero et al 1 report failure to detect Simian virus 40 (SV40) DNA in 12 British mesotheliomas. They propose that their ...
Closed-circular, superhelical DNA from simian virus 40 (SV40 DNA I) was treated with an excess of DNA-relaxing enzyme in the ... Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. W Keller ... Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis ... Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis ...
simian virus 40;. Tag,. SV40 large tumor antigen;. tag,. SV40 small tumor antigen;. EM,. electron microscopy;. HF,. human ... Recently, simian virus 40 (SV40) has been associated with human mesothelioma and brain and bone tumors (reviewed in refs. 1 and ... Simian Virus 40 Small Tumor Antigen Activates AKT and Telomerase and Induces Anchorage-Independent Growth of Human Epithelial ... Simian Virus 40 Infection Down-Regulates the Expression of Nitric Oxide Synthase in Human Mesothelial Cells ...
Cellular transformation by Simian Virus 40 and Murine Polyoma Virus T antigens.. Cheng J1, DeCaprio JA, Fluck MM, Schaffhausen ... Simian Virus 40 (SV40) and Mouse Polyoma Virus (PY) are small DNA tumor viruses that have been used extensively to study ... Cellular Transformation by Simian Virus 40 and Murine Polyoma Virus T antigens ... Cellular Transformation by Simian Virus 40 and Murine Polyoma Virus T antigens ...
... prostate cancer subtypes of greatest malignancy. - GreenMedInfo ... gene signature from multiple transgenic models of epithelial cancers intrinsic to the functions of the Simian virus 40 T/t- ...
... added to agar overlays during plaque assays of simian virus 40 (SV40) in CV1 monkey cells increases the plaque size and number ... Dimethyl sulfoxide (DMSO) added to agar overlays during plaque assays of simian virus 40 (SV40) in CV1 monkey cells increases ... Previous Document: Type-C RNA virus gene expression in human tissue.. Next Document: In vitro radioisotopic labeling of ... 21144544 - Orf3 of porcine circovirus 2 enhances the in vitro and in vivo spread of the of the virus.. 8680524 - Genetic ...
Buy our Recombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein. Ab82118 is a full length protein produced in ... Recombinant-Simian-Virus-40-SV40-SV40-Large-T-Antigen-protein-ab82118.pdf ...
DNA tumor viruses proliferate by hijacking their host cells DNA repli...In the Journal of Biological Chemistry paper Dr. ... The DNA tumor virus simian virus 40 produces the Large T antigen which...The Fbw7 gene is located in a chromosomal region that ... The DNA tumor virus simian virus 40 produces the Large T antigen which...The Fbw7 gene is located in a chromosomal region that ... The DNA tumor virus simian virus 40 produces the Large T antigen which inactivates two of the cells most important cancer- ...
... Li J.J., Kelly ... system derived from monkey cells that is capable of replicating exogenous plasmid DNA molecules containing the simian virus 40 ...
Simian virus 40 (SV40) is associated with some systemic non-Hodgkins lymphomas (NHL) among HIV-positive patients, based on ... Simian virus 40 tumor antigen expression and immunophenotypic profile of AIDS-related non-Hodgkins lymphoma Virology. 2005 Nov ... Simian virus 40 (SV40) is associated with some systemic non-Hodgkins lymphomas (NHL) among HIV-positive patients, based on ... A germinal center B-cell-like (GCB) profile was more frequently expressed by SV40-positive tumors than in Epstein-Barr virus ( ...
Co-operation of simian virus 40 T antigen and insulin receptor substrate-1 in protection from apoptosis induced by interleukin- ... or the Simian virus 40 large T antigen, singly, they still undergo apoptosis after IL-3 withdrawal, although IRS-1 offers ...
Amino Acid and Sugar Transport in Cells Permissively Infected with Simian Virus 40. Michael S. Miller, Lester Kwock and Donald ... The absence of transport enhancement in permissive simian virus 40 infection suggests that the augmented transport of viral- ... Amino Acid and Sugar Transport in Cells Permissively Infected with Simian Virus 40 ... Amino Acid and Sugar Transport in Cells Permissively Infected with Simian Virus 40 ...
Crystal structure of the full-length simian virus 40 small t antigen complexed with the protein phosphatase 2A Aalpha subunit. ...
A monkey virus, Simian Virus 40, could cause non-Hodgkins lymphoma in humans; the virus, which was introduced into human ... A monkey virus, Simian Virus 40, could cause non-Hodgkins lymphoma in humans; the virus, which was introduced into human ...
Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells. Olle Larsson and Britt- ... Effects of treatment with serum-free medium and 25-hydroxycholesterol (25-OH) on the cell cycle of simian virus 40-transformed ... Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells ... Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells ...
Simian virus 40 large T antigen (SV40LTAg) primer specific DNA amplification in human pleural mesothelioma tissue. ... Simian virus 40 large T antigen (SV40LTAg) primer specific DNA amplification in human pleural mesothelioma tissue. ... BACKGROUND: DNA sequences and immunoreactivity associated with Simian virus 40 transforming factors, large T and small t ...
Synthesis of Superhelical Simian Virus 40 Deoxyribonucleic Acid in Cell Lysates* DePamphilis, M. L. ; Beard, P. ; Berg, P. ... Home , Synthesis of Superhelical Simian Virus 40 Deoxyribonucleic Acid in Cell Lysates* ...
Simian virus 40 and DNA Replication. Profiles in Science ID:. PDBBBF. NLM ID:. 101584635X4. Genre:. Articles. Language:. ... Bidirectional Replication of Simian Virus 40 DNA. Extent:. 4 pages. Date Issued:. November 1972. Creator:. Danna, Kathleen J. ( ... Bidirectional Replication of Simian Virus 40 DNA. Proceedings of the National Academy of Sciences of the United States of ...
Primer-DNA formation during simian virus 40 DNA replication in vitro. Message Subject (Your Name) has forwarded a page to you ... Studies of simian virus 40 (SV40) DNA replication in vitro have identified a small (approximately 30-nucleotide) RNA-DNA hybrid ... Primer-DNA formation during simian virus 40 DNA replication in vitro.. D Denis, P A Bullock ...
Immortalization of human fibroblasts transformed by origin-defective simian virus 40.. D S Neufeld, S Ripley, A Henderson, H L ... Immortalization of human fibroblasts transformed by origin-defective simian virus 40.. D S Neufeld, S Ripley, A Henderson, H L ... Immortalization of human fibroblasts transformed by origin-defective simian virus 40.. D S Neufeld, S Ripley, A Henderson, H L ... Simian virus 40 (SV40)-mediated transformation of human diploid fibroblasts has provided an effective experimental system for ...
Therefore, we analyzed the efficacy of a recombinant simian virus 40 vector (rSV40) encoding IGF-I (rSVIGF-I) to prevent ... Liver transduction with a simian virus 40 vector encoding insulin-like growth factor I reduces hepatic damage and the ...
Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ... Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ... Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ... Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ...
Serum Antibodies to JC Virus, BK Virus, Simian Virus 40, and the Risk of Incident Adult Astrocytic Brain Tumors. Dana E. M. ... Genomic sequences of the human polyomaviruses, JC virus (JCV) and BK virus (BKV), and simian virus 40 (SV40) have been reported ... Serum Antibodies to JC Virus, BK Virus, Simian Virus 40, and the Risk of Incident Adult Astrocytic Brain Tumors ... 3 The abbreviations used are: JCV, JC virus; BKV, BK virus; SV40, simian virus 40; ICD, International Classification of ...
Functional Cooperation of Simian Virus 40 Promoter Factor 1 and CCAAT/Enhancer-Binding Protein β and δ in Lipopolysaccharide- ... Functional Cooperation of Simian Virus 40 Promoter Factor 1 and CCAAT/Enhancer-Binding Protein β and δ in Lipopolysaccharide- ... Functional Cooperation of Simian Virus 40 Promoter Factor 1 and CCAAT/Enhancer-Binding Protein β and δ in Lipopolysaccharide- ... Functional Cooperation of Simian Virus 40 Promoter Factor 1 and CCAAT/Enhancer-Binding Protein β and δ in Lipopolysaccharide- ...
Transformation of primary rat kidney cells by fragments of simian virus 40 DNA.. P J Abrahams, C Mulder, A Van De Voorde, S O ... Linear simian virus 40 (SV40) DNA molecules of genome length and DNA fragments smaller than genome length when prepared with ... Infectious virus was only recovered from the cells transformed by circular form I DNA. No infectious virus could be isolated ... Transformation of primary rat kidney cells by fragments of simian virus 40 DNA. ...
You searched for: Subject DNA Cleavage Remove constraint Subject: DNA Cleavage Subject Simian virus 40 Remove constraint ... A Colinear Map Relating the Simian Virus 40 (SV40) DNA Segments of Six Adenovirus-SV40 Hybrids to the DNA Fragments Produced by ... Specific Cleavage of Simian Virus 40 DNA by Restriction Endonuclease of Hemophilus Influenzae ...
  • The genome of Simian Virus 40 (SV40) is a covalently closed circular DNA duplex with about 5200 nucleotide pairs. (springer.com)
  • Mulatero et al 1 report failure to detect Simian virus 40 (SV40) DNA in 12 British mesotheliomas. (bmj.com)
  • Closed-circular, superhelical DNA from simian virus 40 (SV40 DNA I) was treated with an excess of DNA-relaxing enzyme in the presence of increasing amounts of ethidium bromide (EtdBr). (pnas.org)
  • Mesothelioma, a malignancy associated with asbestos, has been recently linked to simian virus 40 (SV40). (pnas.org)
  • Recently, simian virus 40 (SV40) has been associated with human mesothelioma and brain and bone tumors (reviewed in refs. (pnas.org)
  • Simian Virus 40 (SV40) and Mouse Polyoma Virus (PY) are small DNA tumor viruses that have been used extensively to study cellular transformation. (nih.gov)
  • Dimethyl sulfoxide (DMSO) added to agar overlays during plaque assays of simian virus 40 (SV40) in CV1 monkey cells increases the plaque size and number and enables plaques to be read several days earlier than usual. (biomedsearch.com)
  • It does not act synergistically in determining virus inactivation with UV light and is equally effective on wild type and a late mutant of SV40. (biomedsearch.com)
  • Simian virus 40 (SV40) accomplishes this task by producing the highly oncogenic large T antigen. (bio-medicine.org)
  • We recently described a soluble cell-free system derived from monkey cells that is capable of replicating exogenous plasmid DNA molecules containing the simian virus 40 (SV40) origin of replication (J.J. Li, and T.J. Kelly, Proc. (uniprot.org)
  • Simian virus 40 (SV40) is associated with some systemic non-Hodgkin's lymphomas (NHL) among HIV-positive patients, based on assays for viral DNA sequences. (nih.gov)
  • Studies of simian virus 40 (SV40) DNA replication in vitro have identified a small (approximately 30-nucleotide) RNA-DNA hybrid species termed primer-DNA. (asm.org)
  • Simian virus 40 (SV40)-mediated transformation of human diploid fibroblasts has provided an effective experimental system for studies of both "senescence" in cell culture and carcinogenesis. (asm.org)
  • Genomic sequences of the human polyomaviruses, JC virus (JCV) and BK virus (BKV), and simian virus 40 (SV40) have been reported from several types of human brain tumors, but there have been no population-based seroepidemiologic studies to evaluate the association between polyomavirus infection and brain tumors. (aacrjournals.org)
  • Linear simian virus 40 (SV40) DNA molecules of genome length and DNA fragments smaller than genome length when prepared with restriction endonucleases and tested for transforming activity on primary cultures of baby rat kidney cells. (asm.org)
  • Permissive and nonpermissive simian virus 40 (SV40)-infected cells were ultrastructurally analyzed. (asm.org)
  • When compact simian virus 40 (SV40) minichromosomes are treated with staphylococcal nuclease at 0 °C under limit-digest conditions, about one-third of the minichromosomes remain resistant to nuclease, a third of them are nicked, while the remaining third suffer one and only one double-stranded cut. (caltech.edu)
  • The primary transforming functions of simian virus 40 large T antigen (SV40 LT) are conferred primarily through the binding and inactivation of p53 and the retinoblastoma family members. (thebiogrid.org)
  • Whereas the Epstein-Barr virus (EBV) origin (oriP) did little to augment expression from an integrase mutant reporter virus in EBV nuclear antigen 1-expressing cells, simian virus 40 (SV40) oriT dramatically enhanced integrase mutant infectivity in T-antigen (Tag)-expressing cells. (harvard.edu)
  • Adding Tag to integrase mutant-oriT viruses yielded 11.3-kb SV40-HIV chimeras that replicated in Jurkat cells and primary monocyte-derived macrophages. (harvard.edu)
  • Many investigators detected the simian polyomavirus SV40 footprints in human brain tumors and neurologic diseases and recently it has been indicated that SV40 seems to be associated with multiple sclerosis (MS) disease. (eurekamag.com)
  • protein) complexes from simian-virus-40 (SV40)-infected cells late in infection contain 7-10% RNA sequences specific to SV40 DNA. (pasteur.fr)
  • Simian virus 40 (SV40) large tumor antigen (T antigen) unwinds DNA containing the SV40 origin of replication. (semanticscholar.org)
  • Some laboratories have detected DNA from the macaque polyomavirus simian virus 40 (SV40) in human tumors, but possible routes of infection remain unknown. (elsevier.com)
  • The role of simian virus 40 (SV40) in mesothelioma development, on the other hand, remains controversial. (biomedcentral.com)
  • SV40 was introduced unintentionally into millions of people via contaminated poliomyelitis virus vaccines between 1955 and 1963. (biomedcentral.com)
  • We have analysed nucleotide sequences at the junction between simian virus 40 (SV40) and cellular DNA in the Fisher rat transformed line tsA30-N2. (pasteur.fr)
  • Imagine that 20% to 30% of them were contaminated with a brain and lung cancer causing virus called Simian Virus 40 (SV40). (probz.com)
  • PRO MEMBERS get Unlimited Access to downloadable documents like this, with curated research on Simian virus 40 (SV40). (greenmedinfo.com)
  • This downloadable document (GMI PUB) is filled with medically researched knowledge, relevant information and pertinent data on Simian virus 40 (SV40) . (greenmedinfo.com)
  • This GMI PUB document will greatly reduce your research time due to the Cumulative Knowledge feature, and contains a condensed form of the studies that we have accumulated on Simian virus 40 (SV40). (greenmedinfo.com)
  • Some mesothelioma cases show a possible connection between mesothelioma and simian virus 40 (SV40). (mesothelioma.com)
  • The replication of simian virus 40 (SV40) DNA is dependent upon a single viral protein [tumor (T) antigen] and multiple cellular proteins. (duke.edu)
  • The efficiency of simian virus 40 (SV40) DNA replication is dependent on the structural organization of the regulatory region. (ntnu.edu.tw)
  • The simian virus 40 (SV40) large-T antigen is essential for SV40 DNA replication and for late viral gene expression, but the role of the SV40 small-t antigen in these processes is still unclear. (meta.org)
  • Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. (mesothelioma-line.com)
  • MHC class I molecules are a necessary component of the cell surface receptor for simian virus 40 (SV40). (umass.edu)
  • Simian virus 40 (SV40) mRNA was isolated by hybridization of cytoplasmic RNA, from SV40-infected BS-C-1 monkey cells early in lytic infection, to SV40 DNA immobilized on Sepharose. (pubfacts.com)
  • Several studies have detected Simian virus 40 (SV40) deoxyribonucleic acid sequences in human tumor tissues, including lymphomas, mainly by the polymerase chain reaction, but these data were not confirmed by subsequent investigations. (unibas.ch)
  • Two upcoming studies by scientists at the National Cancer Institute (NCI), provide further evidence that exposure to simian virus 40 (SV40) is not associated with cancer in humans. (news-medical.net)
  • It contains an insertion of simian virus 40 (SV40) sequences 3.2 × 103 bases long in place of the 3.5 × 103 base segment of adenovirus 2 DNA which maps between 0.64 and 0.74 fractional genome lengths from the left end of the viral DNA. (cshl.edu)
  • A comparison of the proteins synthesized in human cells at late times after infection with adenovirus (Ad2) and with the adeno-simian virus 40 (SV40) hybrid viruses revealed polypeptides of 30,000 and 92,000 molecular weight specific for the hybrid viruses Ad2+ND1 and Ad2+ND4, respectively. (utah.edu)
  • Cell-free translation of SV40-specific mRNA, prepared from these cells by hybridization of total cytoplasmic RNA to SV40 DNA, showed that the mRNA's specifying these two polypeptides were at least partially encoded by the SV40 portion of the hybrid viruses. (utah.edu)
  • Early versions of the polio vaccine were found to be contaminated with SV40, which led to widespread alarm when tests showed that hamsters infected with the virus grew cancerous tumors. (historyofvaccines.org)
  • From 1955 to 1963, an estimated 10-30% of polio vaccines administered in the US were contaminated with simian virus 40 (SV40). (cdc.gov)
  • Because of research done with SV40 in animal models, there has been some concern that the virus could cause cancer in humans. (cdc.gov)
  • No vaccines used today contain SV40 virus. (cdc.gov)
  • Fibroblasts from patients with xeroderma pigmentosum (XP) complementation groups A, C, D, E, and G, as well as Bloom syndrome (BS) and Fanconi anemia (FA) have been transfected with a plasmid, pSV7, containing the early region of Simian virus 40 (SV40). (elsevier.com)
  • The initiation of simian virus 40 (SV40) replication requires recognition of the viral origin of replication (ori) by SV40 T antigen, followed by denaturation of ori in a reaction dependent upon human replication protein A (hRPA). (researchwithnj.com)
  • Simian virus 40 (SV40), a polyomavirus , was discovered as a contaminant of a human polio vaccine in the 1960s. (bvsalud.org)
  • It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos . (bvsalud.org)
  • Upon infection of the cells by simian virus 40 (SV40), overall transcription of these and other cellular RNAs was stimulated. (elsevier.com)
  • To investigate whether simian virus 40 (SV40) was related to patients of malignant mesothelioma in China . (bvsalud.org)
  • During the past 4 years, polymerase chain reaction (PCR) assays have detected DNA sequences related to SV40 (an oncogenic simian polyomavirus) in a variety of human tissues, especially choroid plexus tumors, ependymomas, mesotheliomas, and osteosarcomas ( 1 - 7 ). (cdc.gov)
  • researchers noted the ability of the PCR primers used in these assays to amplify DNA sequences from JC and BK viruses as well as from SV40 and discussed whether each set of primers in the PCR reaction requires specific conditions to amplify virus-specific DNA. (cdc.gov)
  • Polyclonal antibodies were used to assay human embryonal carcinoma (EC), differentiating EC, yolk sac carcinoma, and teratoma cells for expression of viral early antigen (T-Ag) after infection with simian virus 40 (SV40). (uthscsa.edu)
  • Also, viral late proteins were produced by EC cells infected with human adenovirus type 7 (Ad7), and SV40 T-Ag was expressed by EC cells after infection with PARA, which is an Ad7-SV40 hybrid virus containing the SV40 T-Ag sequence controlled by Ad7 late regulatory sequences. (uthscsa.edu)
  • These results demonstrate that expression of T-Ag after infection with SV40 is an indicator of EC cell differentiation and also raise the possibility that, as in mouse EC cells infected with the virus, the SV40 regulatory sequences controlling T-Ag synthesis are not active in human EC cells. (uthscsa.edu)
  • Simian virus 40 (SV40) was an accidental contaminant of vaccines produced in monkey kidney tissue cultures in the 1950s and early 1960s, including a parenteral adenovirus vaccine given to several hundred thousand US military recruits. (oup.com)
  • Background: Poliovirus vaccines that were used during the late 1950s and early 1960s were contaminated with simian virus 40 (SV40), a monkey virus that is tumorigenic in rodents. (elsevier.com)
  • The 3 termini of this early hybrid virus RNA maps in the vicinity of 0.18 on the conventional SV40 map and probably terminates at the same position as early lytic SV40 cytoplasmic RNA. (princeton.edu)
  • Therefore, the base sequence in this region of SV40 DNA specifies the 3 termini of early messenger RNA present in both hybrid virus and SV40 infected cells. (princeton.edu)
  • Because of the high homology that exists between the DNA of SV40 and the DNA of the JC or BK viruses, the identity of the band obtained by PCR and visualized by ethidium bromide staining was confirmed by both Southern blot hybridization and DNA sequencing. (levelofhealth.com)
  • There were no changes in the ability of the mutant p53s to express the conformation-dependent epitope for monoclonal antibody PAb246 or to participate in complexes with the simian virus 40 (SV40) large T antigen. (dundee.ac.uk)
  • Because viruses may spread more efficiently and be more pathogenic in immunocompromised individuals, we also tested for SV40 lymphoproliferative disorders that had developed in immunocompromised patients. (levelofhealth.com)
  • Simian vacuolating virus 40, known as SV40, is a virus found in monkeys and humans, which has the potential to cause cancer, in animals and humans. (wikipedia.org)
  • the technique used to inactivate the polio virus in the Salk vaccine, by means of formaldehyde, did not reliably kill SV40. (wikipedia.org)
  • It is unknown how widespread the virus was among humans before the 1950s, though one study found that 12% of a sample of German medical students in 1952 - prior to the advent of the vaccines - had SV40 antibodies. (wikipedia.org)
  • abstract = "During normal maturation the majority of pulse-labeled simian virus 40 DNA progresses from chromatin to previrions and virions within 5 h. (elsevier.com)
  • abstract = "Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. (elsevier.com)
  • Cellular transformation by Simian Virus 40 and Murine Polyoma Virus T antigens. (nih.gov)
  • In this study, we identify an integrated gene signature from multiple transgenic models of epithelial cancers intrinsic to the functions of the Simian virus 40 T/t-antigens that is associated with the biological behavior and prognosis for several human epithelial tumors. (greenmedinfo.com)
  • BACKGROUND: DNA sequences and immunoreactivity associated with Simian virus 40 transforming factors, large T and small t antigens (SV40LTAg), suggestive of an aetiopathogenetic link have been identified in fresh frozen tissue of a high proportion of recent cases of pleural mesotheliomas from the United States, Italy and Germany. (bmj.com)
  • Wild-type and J domain mutant simian virus 40 large T antigens alter the cell cycle and bud morphology of Saccharomyces cerevisiae. (mysciencework.com)
  • For example, the immune system's response to antigens that are part of bacteria and viruses helps people resist infections. (mesothelioma-line.com)
  • Simian virus 40 small T antigen activates AMPK and triggers autophagy to protect cancer cells from nutrient deprivation. (semanticscholar.org)
  • Fu, XY, Colgan, JD & Manley, JL 1988, ' Multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mRNA ', Molecular and cellular biology , vol. 8, no. 9, pp. 3582-3590. (elsevier.com)
  • Deletion mutations in the small t antigen gene alter the tissue specificity of tumors induced by simian virus 40. (core.ac.uk)
  • The polyomavirus family includes two human viruses, JCV 3 and BKV. (aacrjournals.org)
  • Currently, viruses in this family that infect humans are related to the lymphotropic polyomavirus (LPV). (cdc.gov)
  • Simian virus 40 infection is associated with breast, lung, and prostate cancer subtypes of greatest malignancy. (greenmedinfo.com)
  • Transport of α-aminoisobutyric acid and 2-deoxy- d -glucose in African green monkey kidney cells was measured 8 to 100 hr following permissive simian virus 40 infection. (aacrjournals.org)
  • The absence of transport enhancement in permissive simian virus 40 infection suggests that the augmented transport of viral-transformed cell lines devolves upon altered host genome function. (aacrjournals.org)
  • Infection of human diploid cells with Simian virus 40 (SV 40) leads to an abortive virus cycle characterized by little virus production, little or no cell destruction, no inhibition of cell replicating capacity, low efficiency of induction of tumour antigen (T-antigen) and eventual transformation of the morphological, chromosomal, and growth characteristics of the cells (Koprowski et al. (microbiologyresearch.org)
  • In marked contrast, in stationary-phase primary African green monkey kidney (GMK) cells, SV 40 infection leads to high yields of virus, highly efficient induction of T-antigen and extensive cell destruction (Carp & Gilden, 1966). (microbiologyresearch.org)
  • CV1 monkey cell infection experiments indicated that mutant viruses that lack small t replicate less efficiently than the wild-type virus. (meta.org)
  • Simian virus 40 infection via MHC class I molecules and caveolae. (umass.edu)
  • Presence of nucleic acid or antigen in semen does not represent the presence of replication-competent or infection-competent virus, which can generally only be demonstrated by isolation and culture of virus. (cdc.gov)
  • Maximum detection time refers to time from symptom onset (only in viruses that cause acute only, not chronic, infection). (cdc.gov)
  • We have compared the cytoplasmic RNA synthesized during both the early and late stages of lytic infection of human cells by these hybrid viruses to that expressed in Ad2 infected and SV 40 infected cells. (princeton.edu)
  • The segment of Ad2 DNA deleted from both hybrid virus genomes is transcribed into cytoplasmic mRNA during the early phase of Ad2 infection. (princeton.edu)
  • Fingerprint Dive into the research topics of 'Simian virus 40 DNA replication: functional organization of regulatory elements. (ntnu.edu.tw)
  • Fingerprint Dive into the research topics of 'Denaturation of the simian virus 40 origin of replication mediated by human replication protein A'. Together they form a unique fingerprint. (researchwithnj.com)
  • Fingerprint Dive into the research topics of 'Interaction of Small Nuclear Ribonucleoproteins with Simian Virus 40 in CV-1 Cells: Is U2 snRNA Involved in Regulating Replication? (elsevier.com)
  • Serum antibodies to JC virus, BK virus, simian virus 40, and the risk of incident adult astrocytic brain tumors. (greenmedinfo.com)
  • Simian virus 40 is found at far higher rates in breast tumors and probably contributes to pathogenesis in those tumors. (greenmedinfo.com)
  • The study of DNA tumors viruses has been an extremely important tool in understanding the cellular pathways that regulate cell division and are disrupted in cancer. (bio-medicine.org)
  • Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro. (semanticscholar.org)
  • To examine the relationships between cardiocyte proliferation and differentiation, we tested the ability of a fragment from the rat β myosin heavy-chain (MHCβ) gene to correctly target expression of a thermolabile simian virus 40 large tumor antigen allele (tsA58) in the developing mouse. (elsevier.com)
  • Like p300, p400 is a phosphoprotein that binds to the simian virus 40 large tumor antigen (T). In anti-T coimmunoprecipitation experiments, staggered deletions spanning the amino-terminal 250 amino acids of T did not abrogate T binding to either p400 or p300. (elsevier.com)
  • Both coarse and higher resolution mapping experiments using the chiral cobalt complex indicate intriguing conformational variations along the simian virus 40 genome. (caltech.edu)
  • Throughout the simian virus 40 genome, variations in structure delineated with the cobalt complex appear to correlate with regions important for control of gene expression. (caltech.edu)
  • These two positions are located within short direct repeats in the virus genome. (pasteur.fr)
  • Our analysis demonstrates that in both cases, recombination preferentially takes place near short direct repeats in the virus genome. (pasteur.fr)
  • In this study of the skin virome of a patient with MCC, olyomaviridae is a family of nonenveloped viruses using high-throughput sequencing (HTS) and comparing with a circular double-stranded DNA genome. (cdc.gov)
  • The genomes of the two nondefective adenovirus 2/simian virus 40 (Ad2/ SV 40) hybrid viruses, nondefective Ad2/SV 40 hybrid virus 1 (Ad2 + ND 1 ) and nondefective hybrid virus 3 (Ad2 + ND 3 ), were formed by a deletion of about 5% of Ad2 DNA and insertion of part of the SV 40 genome. (princeton.edu)
  • Simian virus 40 in humans. (greenmedinfo.com)
  • But, tainted vaccines were knowingly distributed throughout the country because the doctors believed at the time that animal viruses posed no health threat to humans, that the virus that harms monkeys could not harm humans. (larrysmusings.com)
  • So, humans are not immune to all viruses and all bacteria that also attack and harm animals. (larrysmusings.com)
  • EEE is a mosquito-borne virus that can cause serious, and sometimes deadly, disease in humans and equines. (scienceblogs.com)
  • Researchers have hypothesized that SIVs led to the transmission of HIV (human immunodeficiency virus) in humans. (historyofvaccines.org)
  • 000114841 001__ 114841 000114841 005__ 20181203021031.0 000114841 037__ $$aARTICLE 000114841 245__ $$aCharacterization of a new tissue-specific transcription factor binding to the simian virus 40 enhancer TC-II (NF-kappa B) element 000114841 269__ $$a1992 000114841 260__ $$c1992 000114841 336__ $$aJournal Articles 000114841 500__ $$aSwiss Institute for Experimental Cancer Research, Epalinges. (epfl.ch)
  • Accumulation of p300 mediates transcriptional repression of simian virus 40 enhancer in undifferentiated F9 embryonal carcinoma cells -- Ota et al. (aacrjournals.org)
  • Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. (elsevier.com)
  • These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription. (elsevier.com)
  • Two new defective adenovirus 2/simian virus 40 hybrids (Ad2+ D1 and Ad2 + D2) have been isolated from the population known as Ad2++ HEY (Lewis & Rowe, 1970). (cshl.edu)
  • Linzer, D. I.H. / Synthesis and processing of simian virus 40-specific RNA in adenovirus-infected, simian virus 40-transformed human cells . (princeton.edu)
  • Journal of Virology , 40 (3), 729-734. (elsevier.com)
  • Roman, A & Edenberg, H 1981, ' Ultraviolet irradiation inhibits encapsidation of simian virus 40 chromatin ', Journal of Virology , vol. 40, no. 3, pp. 729-734. (elsevier.com)
  • Meek, DW & Eckhart, W 1990, ' Mutation of the serine 312 phosphorylation site does not alter the ability of mouse p53 to inhibit simian virus 40 DNA replication in vivo ', Journal of Virology , vol. 64, no. 4, pp. 1734-1744. (dundee.ac.uk)
  • The DNA tumor virus simian virus 40 produces the Large T antigen which inactivates two of the cell's most important cancer-preventing proteins, p53 and pRb. (bio-medicine.org)
  • The study of the transforming proteins derived from DNA tumor viruses in experimental models of transformation has provided fundamental insights into the process of cell transformation. (semanticscholar.org)
  • Simian virus 40 late proteins possess lytic properties that render them capable of permeabilizing cellular membranes. (umass.edu)
  • The data suggest that the pool of proteins required for maturation is sufficient to convert one-third of the simian virus 40 DNA molecules labeled in a 10-min pulse (at 33 h postinfection) from chromatin to previrions and virions and is exhausted within 1 h. (elsevier.com)
  • In this study, we attempted the artificial regulation of cell proliferation by protein transduction of the N-terminal domain (1-132 amino acids) of the simian virus 40 large T-antigen (SVLT-N), which inactivates retinoblastoma family proteins but not p53. (elsevier.com)
  • Attempts were made to rescue infectious virus from some of the transformed lines by fusion with permissive BSC-1 cells. (asm.org)
  • Although certain permissive T-cell lines support human immunodeficiency virus type 1 (HIV-1) replication in the absence of functional integrase, most cell lines and primary human cells are nonpermissive for integrase mutant growth. (harvard.edu)
  • Simian virus 40 large T antigen (SV40LTAg) primer specific DNA amplification in human pleural mesothelioma tissue. (bmj.com)
  • Hmeljak J, Kern I, Cör A. No implication of Simian virus 40 in pathogenesis of malignant pleural mesothelioma in Slovenia. (cdc.gov)
  • Denaturation of the simian virus 40 origin of replication mediated by human replication protein A . Molecular and cellular biology , 17 (7), 3876-3883. (researchwithnj.com)
  • This set of primers amplifies the regulatory region and the origin of replication of the virus. (levelofhealth.com)
  • Effects of treatment with serum-free medium and 25-hydroxycholesterol (25-OH) on the cell cycle of simian virus 40-transformed 3T3 fibroblasts, designated SV-3T3 cells, were studied and compared with simultaneous effects on the activity of 3-hydroxy-3-methylglutaryl (HMG) CoA reductase and incorporation of [ 3 H]mevalonic acid into cholesterol, Coenzyme Q, and dolichol. (aacrjournals.org)
  • Immortalization of human fibroblasts transformed by origin-defective simian virus 40. (asm.org)
  • Choi, SK, Lee, SH & Chang, HI 1998, ' Changes of Nuclear Glycoproteins in Normal and Simian Virus 40-Transformed WI-38 Human Lung Fibroblasts ', Molecules and cells , vol. 8, no. 6, pp. 724-730. (elsevier.com)
  • Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication. (uniprot.org)
  • Primer-DNA formation during simian virus 40 DNA replication in vitro. (asm.org)
  • Purification of replication protein C, a cellular protein involved in the initial stages of simian virus 40 DNA replication in vitro. (duke.edu)
  • Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells. (uniprot.org)
  • When 32D cells are stably transfected with plasmids expressing either IRS-1 (a major substrate of the IGF-IR) or the Simian virus 40 large T antigen, singly, they still undergo apoptosis after IL-3 withdrawal, although IRS-1 offers partial protection. (nih.gov)
  • A perifusion system for the study of insulin secretory dynamics of a clonal, Simian virus 40-transformed hamster pancreatic beta cell line (HIT cells) is described. (diabetesjournals.org)
  • Transformation of primary rat kidney cells by fragments of simian virus 40 DNA. (asm.org)
  • Infectious virus was only recovered from the cells transformed by circular form I DNA. (asm.org)
  • No infectious virus could be isolated from any of the other types of transformed cells. (asm.org)
  • Incorporating oriT into the nef position of a full-length, integrase-defective virus strain yielded efficient replication in Tag-expressing nonpermissive Jurkat T cells without reversion to an integration-competent genotype. (harvard.edu)
  • However, recent advances in the propagation of liver cells such as the introduction of temperature sensitive mutant Simian virus 40 (SV-40) T antigen mutants 4 may obviate the need for harvests of large numbers of host cells for genetic manipulation. (bmj.com)
  • Interaction of Small Nuclear Ribonucleoproteins with Simian Virus 40 in CV-1 Cells: Is U2 snRNA Involved in Regulating Replication? (elsevier.com)
  • We observed six major tryptic phosphopeptides in p53 from simian virus 40-transformed and normal NIH 3T3 cells. (dundee.ac.uk)
  • Phosphorylation of serines 310 and/or 312 was twofold higher in the simian virus 40-transformed cells as compared with that in normal NIH 3T3 cells. (dundee.ac.uk)
  • Meek, DW & Eckhart, W 1988, ' Phosphorylation of p53 in normal and simian virus 40-transformed NIH 3T3 cells ', Molecular and Cellular Biology , vol. 8, no. 1, pp. 461-465. (dundee.ac.uk)
  • Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [ 125 I]S3 polysaccharide protein conjugate. (nyu.edu)
  • The contamination may have been in the original seed strain (coded SOM) or in the substrate-primary kidney cells from infected monkeys used to grow the vaccine virus during production. (wikipedia.org)
  • 1998 ) A multi-institutional study confirms the presence and expression of Simian virus 40 in human malignant mesotheliomas. (bmj.com)
  • No association between simian virus 40 and diffuse malignant mesothelioma of the pleura in Iranian patients: a molecular and epidemiologic case-control study of 60 patients. (cdc.gov)
  • No detection of simian virus 40 in malignant mesothelioma in Korea. (cdc.gov)
  • The "tumor antigen" (T antigen), a nonstructural protein expressed by the viruses, is responsible for cell transformation in animal models. (aacrjournals.org)
  • Enumeration of the simian virus 40 early region elements necessary for human cell transformation. (semanticscholar.org)
  • Simian Virus 40 large T antigen is a multi-functional protein that is involved in the initiation of viral DNA replication, regulation of viral transcription and cell transformation. (unthsc.edu)
  • These observations strongly suggest that a normal, committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody. (nyu.edu)
  • Fishman, Glenn I. / Cardiac and skeletal myopathy in β myosin heavy-chain simian virus 40 tsA58 transgenic mice . (elsevier.com)
  • Therefore, we analyzed the efficacy of a recombinant simian virus 40 vector (rSV40) encoding IGF-I (rSVIGF-I) to prevent cirrhosis progression. (nih.gov)
  • This resulted in a 30-40% reduction in serum cholesterol, with the recombinant LDL receptor being detectable for up to six months. (bmj.com)
  • the virus, which was introduced into human populations in the 1950s and 1960s by contaminated polio vaccines, is spread via saliva and other bodily fluids. (harpers.org)
  • The virus came from monkey kidney cell cultures used to make polio vaccines at that time. (cdc.gov)
  • How these viruses initiate the lytic event is largely unknown. (umass.edu)
  • Type-C RNA virus gene expression in human tissue. (biomedsearch.com)
  • Deletion of the carboxy terminus of simian virus 40 large T antigen affects viral late gene expression. (dartmouth.edu)
  • Hence, we suggest that Ad2 codes for at least one 'early' gene product which is nonessential for virus growth in cell culture. (princeton.edu)
  • Nucleotide sequence of the simian virus 40 HindII + III restriction fragment A (second part of the T antigen gene). (cngb.org)
  • DNA tumor viruses proliferate by hijacking their host cell's DNA repli. (bio-medicine.org)
  • DNA tumor viruses proliferate by hijacking their host cell's DNA replication machinery. (bio-medicine.org)
  • New binding target for oncogenic viral protein ( The DNA tumor virus simian virus 40 pro. (bio-medicine.org)
  • Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53. (thebiogrid.org)
  • protein particles for virus nuclear RNA. (pasteur.fr)
  • NFI-X3, a splice variant of NFIX, regulates Glial fibrillary acidic protein and YKL-40 in astrocytes. (wikipedia.org)
  • Understanding the mechanisms through which these viruses interact with the cellular machinery that regulates cell division may lead to new insights into the pathways that cause cancer. (bio-medicine.org)
  • Membrane fusion as a mechanism of simian virus 40 entry into different cellular compartments. (asm.org)
  • Several cellular polypeptides critical for growth regulation interact with DNA tumor virus oncoproteins. (elsevier.com)
  • Studies have shown a strong correlation between those that have the virus and a development of mesothelioma, though further investigation is needed. (mesothelioma.com)
  • Epitopes described in Dissection of H-2Db-restricted cytotoxic T-lymphocyte epitopes on simian virus 40 T antigen by the use of synthetic peptides and H-2Dbm mutants. (iedb.org)
  • Even me, not a doctor, was aware since early 2000 that polio vaccinations between 1955 and 1963 were tainted with this simian virus (from monkeys). (larrysmusings.com)
  • An analysis presented at the Vaccine Cell Substrate Conference in 2004[medical citation needed] suggested that vaccines used in the former Soviet bloc countries, China, Japan, and Africa, could have been contaminated up to 1980, meaning that hundreds of millions more could have been exposed to the virus unknowingly. (wikipedia.org)
  • Co-operation of simian virus 40 T antigen and insulin receptor substrate-1 in protection from apoptosis induced by interleukin-3 withdrawal. (nih.gov)
  • Simian virus 40 has a high prevalence in hematological malignancies and probably plays a role in tumor pathogenesis. (greenmedinfo.com)
  • Since, the estimates range widely from 30 to 98 million people being exposed to SV 40, the reality is that there will, over time, be more cases of cancer than if they had not been exposed to this animal virus. (larrysmusings.com)
  • Patients infected with the hepatitis C virus (HCV) are six times as likely to develop non-Hodgkin's lymphoma (NHL) than individuals that are virus free, according to research presented at the Third Annual Frontiers in Cancer Prevention Research meeting. (news-medical.net)
  • In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent to the transforming activity lost by deletion of the E1A p300-binding region. (cshl.edu)