A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
Deoxyribonucleic acid that makes up the genetic material of viruses.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Viruses whose genetic material is RNA.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Established cell cultures that have the potential to propagate indefinitely.
The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.
Process of growing viruses in live animals, plants, or cultured cells.
The functional hereditary units of VIRUSES.
Substances elaborated by viruses that have antigenic activity.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Proteins found in any species of virus.
The process by which a DNA molecule is duplicated.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
The expelling of virus particles from the body. Important routes include the respiratory tract, genital tract, and intestinal tract. Virus shedding is an important means of vertical transmission (INFECTIOUS DISEASE TRANSMISSION, VERTICAL).
A general term for diseases produced by viruses.
Ribonucleic acid that makes up the genetic material of viruses.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
Viruses parasitic on plants higher than bacteria.
Viruses whose nucleic acid is DNA.
The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.
A species of POLYOMAVIRUS apparently infecting over 90% of children but not clearly associated with any clinical illness in childhood. The virus remains latent in the body throughout life and can be reactivated under certain circumstances.
The type species of MORBILLIVIRUS and the cause of the highly infectious human disease MEASLES, which affects mostly children.
A subtype of INFLUENZA A VIRUS with the surface proteins hemagglutinin 1 and neuraminidase 1. The H1N1 subtype was responsible for the Spanish flu pandemic of 1918.
A genus of potentially oncogenic viruses of the family POLYOMAVIRIDAE. These viruses are normally present in their natural hosts as latent infections. The virus is oncogenic in hosts different from the species of origin.
A family of small, non-enveloped DNA viruses, infecting mainly MAMMALS, and containing a single genus: POLYOMAVIRUS.
The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 5 and neuraminidase 1. The H5N1 subtype, frequently referred to as the bird flu virus, is endemic in wild birds and very contagious among both domestic (POULTRY) and wild birds. It does not usually infect humans, but some cases have been reported.
A species of RUBULAVIRUS originally isolated from cultured primary monkey cells. Its natural host is the DOG in which it causes kennel cough, but it can also infect humans.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.
The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the virions have both HEMAGGLUTININ and NEURAMINIDASE activities and encode a non-structural C protein. SENDAI VIRUS is the type species.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 3 and neuraminidase 2. The H3N2 subtype was responsible for the Hong Kong flu pandemic of 1968.
A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE). It can infect birds and mammals. In humans, it is seen most frequently in Africa, Asia, and Europe presenting as a silent infection or undifferentiated fever (WEST NILE FEVER). The virus appeared in North America for the first time in 1999. It is transmitted mainly by CULEX spp mosquitoes which feed primarily on birds, but it can also be carried by the Asian Tiger mosquito, AEDES albopictus, which feeds mainly on mammals.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The mechanism by which latent viruses, such as genetically transmitted tumor viruses (PROVIRUSES) or PROPHAGES of lysogenic bacteria, are induced to replicate and then released as infectious viruses. It may be effected by various endogenous and exogenous stimuli, including B-cell LIPOPOLYSACCHARIDES, glucocorticoid hormones, halogenated pyrimidines, IONIZING RADIATION, ultraviolet light, and superinfecting viruses.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A group of viruses in the PNEUMOVIRUS genus causing respiratory infections in various mammals. Humans and cattle are most affected but infections in goats and sheep have also been reported.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the species have hemagglutinin and neuraminidase activities but lack a C protein. MUMPS VIRUS is the type species.
Viruses that produce tumors.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A species of RESPIROVIRUS also called hemadsorption virus 2 (HA2), which causes laryngotracheitis in humans, especially children.
A species of POLYOMAVIRUS, originally isolated from the brain of a patient with progressive multifocal leukoencephalopathy. The patient's initials J.C. gave the virus its name. Infection is not accompanied by any apparent illness but serious demyelinating disease can appear later, probably following reactivation of latent virus.
A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.
The type species of VESICULOVIRUS causing a disease symptomatically similar to FOOT-AND-MOUTH DISEASE in cattle, horses, and pigs. It may be transmitted to other species including humans, where it causes influenza-like symptoms.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Membrane glycoproteins from influenza viruses which are involved in hemagglutination, virus attachment, and envelope fusion. Fourteen distinct subtypes of HA glycoproteins and nine of NA glycoproteins have been identified from INFLUENZA A VIRUS; no subtypes have been identified for Influenza B or Influenza C viruses.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A family of spherical viruses, of the order MONONEGAVIRALES, somewhat larger than the orthomyxoviruses, and containing single-stranded RNA. Subfamilies include PARAMYXOVIRINAE and PNEUMOVIRINAE.
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
The ability of a pathogenic virus to lie dormant within a cell (latent infection). In eukaryotes, subsequent activation and viral replication is thought to be caused by extracellular stimulation of cellular transcription factors. Latency in bacteriophage is maintained by the expression of virally encoded repressors.
The type species of RUBULAVIRUS that causes an acute infectious disease in humans, affecting mainly children. Transmission occurs by droplet infection.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Group of alpharetroviruses (ALPHARETROVIRUS) producing sarcomata and other tumors in chickens and other fowl and also in pigeons, ducks, and RATS.
Species of the genus LENTIVIRUS, subgenus primate immunodeficiency viruses (IMMUNODEFICIENCY VIRUSES, PRIMATE), that induces acquired immunodeficiency syndrome in monkeys and apes (SAIDS). The genetic organization of SIV is virtually identical to HIV.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Infections with POLYOMAVIRUS, which are often cultured from the urine of kidney transplant patients. Excretion of BK VIRUS is associated with ureteral strictures and CYSTITIS, and that of JC VIRUS with progressive multifocal leukoencephalopathy (LEUKOENCEPHALOPATHY, PROGRESSIVE MULTIFOCAL).
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Viruses which produce a mottled appearance of the leaves of plants.
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.
Proteins that form the CAPSID of VIRUSES.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A species in the genus HEPATOVIRUS containing one serotype and two strains: HUMAN HEPATITIS A VIRUS and Simian hepatitis A virus causing hepatitis in humans (HEPATITIS A) and primates, respectively.
A species of ALPHAVIRUS isolated in central, eastern, and southern Africa.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A family of RNA viruses causing INFLUENZA and other diseases. There are five recognized genera: INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; ISAVIRUS; and THOGOTOVIRUS.
The relationships of groups of organisms as reflected by their genetic makeup.
Viruses whose taxonomic relationships have not been established.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The sum of the weight of all the atoms in a molecule.
The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
Proteins conjugated with nucleic acids.
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
The type species of ALPHARETROVIRUS producing latent or manifest lymphoid leukosis in fowl.

Human topoisomerase I promotes initiation of simian virus 40 DNA replication in vitro. (1/4150)

Addition of purified human topoisomerase I (topo I) to simian virus 40 T antigen-driven in vitro DNA replication reactions performed with topo I-deficient extracts results in a greater than 10-fold stimulation of completed molecules as well as a more than 3-fold enhancement of overall DNA replication. To further characterize this stimulation, we first demonstrate that bovine topo I but not Escherichia coli topo I can also enhance DNA replication. By using several human topo I mutants, we show that a catalytically active form of topo I is required. To delineate whether topo I influences the initiation or the elongation step of replication, we performed delayed pulse, pulse-chase, and delayed pulse-chase experiments. The results illustrate that topo I cannot promote the completion of partially replicated molecules but is needed from the beginning of the reaction to initiate replication. Competitive inhibition experiments with the topo I binding T antigen fragment 1-246T and a catalytically inactive topo I mutant suggest that part of topo I's stimulation of replication is mediated through a direct interaction with T antigen. Collectively, our data indicate that topo I enhances the synthesis of fully replicated DNA molecules by forming essential interactions with T antigen and stimulating initiation.  (+info)

Induction of AT-specific DNA-interstrand crosslinks by bizelesin in genomic and simian virus 40 DNA. (2/4150)

Bizelesin is a bifunctional AT-specific DNA alkylating drug. Our study characterized the ability of bizelesin to induce interstrand crosslinks, a potential lethal lesion. In genomic DNA of BSC-1 cells, bizelesin formed from approx. 0.3 to 6.03+/-0.85 interstrand crosslinks per 106 base pairs, at 5-100 nM drug concentration, respectively, comparable to the number of total adducts previously determined in the same system (J.M. Woynarowski, M.M. McHugh, L.S. Gawron, T.A. Beerman, Biochemistry 34 (1995) 13042-13050). Bizelesin did not induce DNA-protein crosslinks or strand breaks. A model defined target, intracellular simian virus 40 (SV40) DNA, was employed to map at the nucleotide level sites of bizelesin adducts, including potential interstrand crosslinks. Preferential adduct formation was observed at AT tracts which are abundant in the SV40 matrix associated region and the origin of replication. Many sites, including each occurrence of 5'-T(A/T)4A-3', co-mapped on both DNA strands suggesting interstrand crosslinks, although monoadducts were also formed. Bizelesin adducts in naked SV40 DNA were found at similar sites. The localization of bizelesin-induced crosslinks in AT-rich tracts of replication-related regions is consistent with the potent anti-replicative properties of bizelesin. Given the apparent lack of other types of lesions in genomic DNA, interstrand crosslinks localized in AT-rich tracts, and to some extent perhaps also monoadducts, are likely to be lethal effects of bizelesin.  (+info)

Downregulation of metallothionein-IIA expression occurs at immortalization. (3/4150)

Metallothioneins (MTs) may modulate a variety of cellular processes by regulating the activity of zinc-binding proteins. These proteins have been implicated in cell growth regulation, and their expression is abnormal in some tumors. In particular, MT-IIA is expressed 27-fold less in human colorectal tumors and tumor cell lines compared with normal tissue (Zhang et al., 1997). Here we demonstrate that MT-IIA downregulation occurs when human cells become immortal, a key event in tumorigenesis. After immortalization MT-IIA expression remains inducible but the basal activity of the MT-IIA promoter is decreased. MT-IIA downregulation at immortalization is one of the most common immortalization-related changes identified to date, suggesting that MT-IIA has a role in this process.  (+info)

Association of simian virus 40 with a central nervous system lesion distinct from progressive multifocal leukoencephalopathy in macaques with AIDS. (4/4150)

The primate polyomavirus SV40 is known to cause interstitial nephritis in primary infections and progressive multifocal leukoencephalopathy (PML) upon reactivation of a latent infection in SIV-infected macaques. We now describe a second central nervous system manifestation of SV40: a meningoencephalitis affecting cerebral gray matter, without demyelination, distinct from PML. Meningoencephalitis appears also to be a primary manifestation of SV40 infection and can be seen in conjunction with SV40-induced interstitial nephritis and pneumonitis. The difference in the lesions of meningoencephalitis and PML does not appear to be due to cellular tropism, as both oligodendrocytes and astrocytes are infected in PML and meningoencephalitis, as determined by in situ hybridization or immunohistochemistry for SV40 coupled with immunohistochemistry for cellular determinants. This is further supported by examination of SV40 nucleic acid sequences from the ori-enhancer and large-T-antigen regions, which reveals no tissue-or lesion-specific variation in SV40 sequences.  (+info)

Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. (5/4150)

Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.  (+info)

Increased ultraviolet sensitivity and chromosomal instability related to P53 function in the xeroderma pigmentosum variant. (6/4150)

The xeroderma pigmentosum (XP) variant (XPV) is a form of XP that has normal excision repair but shows defective DNA replication after UV irradiation. In developing various transformed fibroblast cell lines from these patients, we have found that there are significant phenotypic changes in transformed cells that seem to correlate with inactivation of p53. After transformation with SV40, XPV cell lines are only slightly UV sensitive, like their primary counterparts, but their sensitization with caffeine and the induction of sister chromatid exchanges (SCEs) by UV irradiation are greatly enhanced. After transformation by HPV16 E7, which targets the retinoblastoma cell cycle regulatory gene, there is no change in the UV sensitivity of XPV cells; but, when transformed by HPV16 E6 or E6 and E7 combined, there is a large increase in UV sensitivity and in the induction of SCEs. These changes are not associated with any detectable changes in the reactivation of an externally irradiated luciferase expression vector, the excision of cyclobutane pyrimidine dimers from bulk DNA, or unscheduled DNA synthesis and, therefore, do not involve excision repair. We suggest that if SCEs represent homologous recombination between sister chromatids, then in the absence of p53 function, the DNA chain arrest typical of UV-damaged XPV cells initiates strand exchange during recovery. In untransformed cells with normal p53, the preferred mode of recovery would then be replication bypass. The symptoms of elevated solar carcinogenesis in XPV patients may, therefore, be associated with increased genomic instability in cells of the skin in which p53 is inactivated by UV-induced mutations.  (+info)

Use of the Gal4-UAS technique for targeted gene expression in the zebrafish. (7/4150)

The most common way to analyze the function of cloned genes in zebrafish is to misexpress the gene product or an altered variant of it by mRNA injection. However, mRNA injection has several disadvantages. The GAL4-UAS system for targeted gene expression allows one to overcome some of these disadvantages. To test the GAL4-UAS system in zebrafish, we generated two different kinds of stable transgenic lines, carrying activator and effector constructs, respectively. In the activator lines the gene for the yeast transcriptional activator GAL4 is under the control of a given promoter, while in the effectors the gene of interest is fused to the sequence of the DNA-binding motif of GAL4 (UAS). Crosses of animals from the activator and effector lines show that effector genes are transcribed with the spatial pattern of the activators. This work smoothes the way for a novel method of misexpression of gene products in zebrafish in order to analyze the function of genes in developmental processes.  (+info)

The simian virus 40 small-t and large-T antigens jointly regulate cell cycle reentry in human fibroblasts. (8/4150)

Focus formation in human diploid fibroblasts (HDF cells) is known to require both the simian virus 40 (SV40) large-T and small-t antigens. Similarly, both SV40 proteins were required to stimulate confluent, density-arrested HDF cells to reenter the cell cycle. This study used defective recombinant adenoviruses to examine the roles of the individual SV40 proteins in altering specific steps in the cell cycle. Small-t antigen and, to a lesser extent, large-T antigen increased the level of the S phase cyclin cyclin A but without increasing the activity of associated cyclin kinases unless the two SV40 proteins were coexpressed. The absence of kinase activity reflected the presence in density-arrested cells of high levels of the cyclin-dependent kinase inhibitors p21(WAF1) and p27(KIP1). We report here that expression of SV40 large-T antigen reduced levels of p21(WAF1), while expression of small-t antigen was required to decrease p27(KIP1). The separate effects of large-T and small-t antigens on these two inhibitors may explain the joint requirement for the two proteins to drive cell cycle reentry of HDF cells and ultimately transform these cells.  (+info)

Simian virus (SV40) large T antigen, molecular model. This antigen is from the simian vacuolating virus 40 (SV40). Large T antigens play a role in regulating the viral life cycle of the polyomaviridae viruses, such as SV40. SV40 is found in monkeys such as Rhesus monkeys and macaques. Potentially tumour-causing in primates and humans, it is used in laboratory research and in vaccines. - Stock Image C025/1808
This topic contains 11 study abstracts on Simian virus 40 (SV40) indicating it may contribute to Simian virus 40 (SV40), Mesothelioma, and Cancer Metastasis
Simian virus 40 (SV40)-mediated transformation of human diploid fibroblasts has provided an effective experimental system for studies of both senescence in cell culture and carcinogenesis. Previous interpretations may have been complicated, however, by the semipermissive virus-cell interaction. In earlier studies, we previously demonstrated that the human diploid fibroblast line HS74 can be efficiently transformed by DNA from replication-defective mutants of SV40 containing a deletion in the viral origin for DNA synthesis (SVori-). In the current study, we found that such SVori- transformants show a significantly increased life span in culture, as compared with either HS74 or an independent transformant containing an intact viral genome, but they nonetheless undergo senescence. We have clonally isolated six immortalized derivatives of one such transformant (SV/HF-5). Growth studies indicate that the immortalized cell lines do not invariably grow better than SV/HF-5 or HS74. Genetic studies ...
The oligomers formed by a mutant nonkaryophilic large T antigen of simian virus 40, which lacks residues 110 through 152 of normal large T antigen and transforms only established cells (L. Fischer-Fantuzzi and C. Vesco, Proc. Natl. Acad. Sci. USA 82:1891-1895, 1985), were found to consist predominantly of dimers. Anti-p53 antibodies precipitated 14 to 16S complexes containing the mutant nonkaryophilic large T antigen and p53 from extracts of transformed cells, and anti-p53 indirect immunofluorescence stained these cells in the cytoplasm. ...
Buy our Recombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein. Ab82118 is a full length protein produced in Baculovirus and has been validated in…
The role of simian virus 40 (SV40) large tumor antigen (T antigen) as a DNA helicase at the replication fork was studied. We found that a T-antigen hexamer complex acts during the unidirectional unwinding of appropriate DNA substrates and is localized directly in the center of the fork, contacting the adjacent double strand as well as the emerging single strands. When bidirectional DNA unwinding, initiated at the viral origin of DNA replication, was analyzed, a larger T-antigen complex that is simultaneously active at both branch points of an unwinding bubble was observed. The size and shape of this helicase complex imply that the T-antigen dodecamer complex, assembled at the origin and active in the localized melting of duplex DNA, is subsequently also used to continue DNA unwinding bidirectionally. Then, however, the dodecamer complex does not split into two hexamer subunits that track along the DNA; rather, the DNA is threaded through the intact complex, with the concomitant extrusion of ...
You searched for: Creator Nathans, Daniel, 1928-1999 Remove constraint Creator: Nathans, Daniel, 1928-1999 Creator Lee, Theresa N. H. (Theresa N. H. Lee) Remove constraint Creator: Lee, Theresa N. H. (Theresa N. H. Lee) Subject DNA Cleavage Remove constraint Subject: DNA Cleavage Subject Simian virus 40 Remove constraint Subject: Simian virus 40 ...
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Deppert, W and Walter, G, Domains of simian virus 40 large t-antigen exposed on the cell surface. (1982). Subject Strain Bibliography 1982. 3601 ...
The genome of Simian Virus 40 (SV40) is a covalently closed circular DNA duplex with about 5200 nucleotide pairs. Based on physiological studies and mapping of temperature-sensitive and deletion...
We have analysed nucleotide sequences at the junction between simian virus 40 (SV40) and cellular DNA in the Fisher rat transformed line tsA30-N2. This line
simian virus 40, polio vaccines, and cancer I take no pleasure in these essays that expose the poor judgment and even recklessness of the US medical establishment. But, facts are facts, albeit unpleasant in many instances. (Update: Summer, 2014. We have recently heard that the introduction of anti-biotics in the early 1940s also seems to…
This entry was posted on June 2, 2011, 3:42 am and is filed under Fugitive Document. You can follow any responses to this entry through RSS 2.0. You can leave a response, or trackback from your own site. ...
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in Research in Veterinary Science (2009), 87(1), 123-32. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be ... [more ▼]. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be distinguished from simultaneously isolated colonocytes, as the jejunocytes specifically exhibited the small intestinal peptidase gene transcript, as well as an active alkaline phosphatase. The transformation of both types of cell suspension was performed by retroviral infection, using reproduction-defective viruses bearing the gene coding for the large T antigen of the leukaemia simian virus (SV40). The success of the transfection was demonstrated by (1) a significant increase in cell passage numbers (52-53 vs. 7 passages ...
4FGN: Analysis of the Costructure of the Simian Virus 40 T-Antigen Origin Binding Domain with Site I Reveals a Correlation between GAGGC Spacing and Spiral Assembly.
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TY - JOUR. T1 - Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription. AU - Nakagoshi, H.. AU - Nagase, T.. AU - Kanei-Ishii, C.. AU - Ueno, Y.. AU - Ishii, S.. PY - 1990/3/23. Y1 - 1990/3/23. N2 - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequene. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.. AB - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The ...
TY - JOUR. T1 - Transformation of DNA repair-deficient human diploid fibroblasts with a simian virus 40 plasmid. AU - Wood, C. M.. AU - Timme, T. L.. AU - Hurt, M. M.. AU - Brinkley, B. R.. AU - Ledbetter, D. H.. AU - Moses, R. E.. PY - 1987/4. Y1 - 1987/4. N2 - Fibroblasts from patients with xeroderma pigmentosum (XP) complementation groups A, C, D, E, and G, as well as Bloom syndrome (BS) and Fanconi anemia (FA) have been transfected with a plasmid, pSV7, containing the early region of Simian virus 40 (SV40). All of the cultures exhibited cytologic changes characteristic of transformed cells and expressed T-antigen. They also contained integrated copies of DNA derived from the vector, and in several cases, extrachromosomally replicated DNA. Not all of the transfected cultures became immortalized. The transformed xeroderma pigmentosum (XP) cultures retained their UV-sensitive phenotype in all but one case. The BS and FA cell lines retained their characteristic phenotype. All of the cultures, ...
DELETION OF THE CARBOXY TERMINUS OT SIMIAN VIRUS 40 LARGE T ANTIGEN AFFECTS VIRAL LATE GENE EXPRESSION A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy Terryl Stacy DARTMOUTH COLLEGE Hanover, New Hampshire March 8,1990 ...
Li J.J., Kelly T.J.. We recently described a soluble cell-free system derived from monkey cells that is capable of replicating exogenous plasmid DNA molecules containing the simian virus 40 (SV40) origin of replication (J.J. Li, and T.J. Kelly, Proc. Natl. Acad. Sci. U.S.A. 81:6973-6977, 1984). Replication in the system is completely dependent upon the addition of the SV40 large T antigen. In this report we describe additional properties of the in vitro replication reaction. Extracts prepared from cells of several nonsimian species were tested for the ability to support origin-dependent replication in the presence of T antigen. The activities of extracts derived from human cell lines HeLa and 293 were approximately the same as those of monkey cell extracts. Chinese hamster ovary cell extracts also supported SV40 DNA replication in vitro, but the extent of replication was approximately 1% of that observed with human or monkey cell extracts. No replication activity was detectable in extracts ...
Summary Simian virus 40 (SV40)-transformed cells express the SV40-specific tumour transplantation antigen (TSTA) on the cell surface and the SV40-coded tumour antigen in their nuclei. TSTA is defined by SV40-specific transplantation immunity, whereas T-antigen (T-Ag) can be detected serologically by indirect immunofluorescence. Both antigens, however, are derived from the A gene of SV40. We therefore analysed SV40-transformed cells for the presence of serologically detectable T-Ag-related molecules. Such antigens could not be detected on the surface of living SV40-transformed cells in monolayers. However, after a short formaldehyde fixation it was possible to stain the cell surfaces of SV40-transformed cells with sera from rabbits immunized with purified SDS-denatured T-Ag, but not with sera from hamsters bearing SV40-induced tumours. T-Ag-related antigens could be detected with both types of antisera by applying a more sensitive 125I-protein A assay. The T-Ag specificity of the binding of hamster SV40
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
The complete nucleotide sequence of the P gene of simian virus 41 (SV41) was determined. The gene was found to be 1406 nucleotides long and to contain a relatively small open reading frame encoding a cysteine-rich V protein with a calculated M r of 24076. We have demonstrated that RNA-editing events occur in SV41 P gene transcripts and that the ratio of edited mRNAs to faithfully copied mRNA (P-mRNA: V-mRNA) is about 1:5 at either 24 or 40 h post-infection. The mRNA with two G insertions was capable of encoding a P protein of 395 amino acids with a predicted M r of 41992. A kinetic study of P and V proteins by Western blot analysis showed that in virus-infected cells the amounts of both proteins were almost equal although the V-mRNA was considerably more abundant than the P-mRNA. Alignment of the SV41 P and V proteins with those of nine other paramyxoviruses demonstrated that irregular gaps were present around the RNA-editing sites.
By Catherine Frompovich After half a century, the 108th U.S. Congress House of Representatives Subcommittee on Human Rights and Wellness finally held a hearing September 10, 2003, on the simian virus (SV40) that was included in the original polio vaccine produced and administered to children in the 1950s and 1960s. Candidly, the first page of ...
human alpha 1-antitrypsin promoter, human immunoglobulin heavy chain genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, Simian virus 40 poly A ...
Chang C, Simmons DT, Martin MA and Mora PT (1979) Identification and partial characterization of new antigens from simian virus 40-transformed mouse cells. J Virol 31: 463-471.. Crawford LV, Pim DC and Bulbrook RD (1982) Detection of antibodies against the cellular protein p53 in sera from patients with breast cancer. Int. J. Cancer 30: 403-408.. Crawford L (1983) The 53,000-dalton cellular protein and its role in transformation. Int. Rev. Exp. Path. 25: 1-50.. De Leo AB, Jay G, Appella E, Dubois GC, Law LW and Old LJ (1979) Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse. Proc Natl Acad Sci USA 76: 2420-2424.. Kress M, May E, Cassingena R and May P (1979) Simian Virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 serum. J. Virol. 31: 472-483.. Lane DP and Crawford LV (1979) T antigen is bound to a host protein in SV40-transformed cells. Nature 278: 261-263.. Linzer DIH and Levine AJ ...
Subramani, S. Rescue of chromosomal T-antigen sequences onto extrachromosomally replicating, defective simian virus 40 DNA by homologous recombination.. Molecular and Cellular Biology 6.4 (1986): 1320-1325. Web. 06 Aug. 2020. ...
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p53 is a cellular-encoded phosphoprotein first identified in protein complexes with the large tumor (T) antigen of simian virus 40 (SV40) (Linzer and Levine, 1979; Lane and Crawford, 1979). High...
A hybrid virus is a type of computer virus that combines elements of other virus programs in a new combination that will often...
Goat polyclonal antibody raised against synthetic peptide of V5 Tag. A synthetic peptide (conjugated with KLH) corresponding to amino acids 95-108 of RNA polymerase alpha of subunit simian virus 5. (PAB14287) - Products - Abnova
An article can be defined as the Most Viewed based on the number of clicks to the page, visits, downloads and sharing from the Journal of Clinical and Cellular Immunology .
A comparison of the proteins synthesized in human cells at late times after infection with adenovirus (Ad2) and with the adeno-simian virus 40 (SV40) hybrid viruses revealed polypeptides of 30,000 and 92,000 molecular weight specific for the hybrid viruses Ad2+ND1 and Ad2+ND4, respectively. Cell-free translation of SV40-specific mRNA, prepared from these cells by hybridization of total cytoplasmic RNA to SV40 DNA, showed that the mRNAs specifying these two polypeptides were at least partially encoded by the SV40 portion of the hybrid viruses. Cell-free translation of SV40-specific mRNA prepared from monkey cells infected with SV40 produced polypeptides of 40,000, 43,000, 48,500, and 92,000 molecular weight. The SV40 and Ad2+ND4 92,000-molecular-weight polypeptides made in vitro were very similar in electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels to the polypeptide precipitated by Tegtmeyer (1974) with SV40 anti-T serum.
Human polyomaviruses (JC virus, BK virus and simian virus 40) are causative agents of some human diseases and, interestingly, are involved in processes of cell transformation and oncogenesis. These viruses need the cell cycle machinery of the host cell to complete their replication; so they evolved mechanisms that can interfere with the growth control of infected cells and force them into DNA replication. The retinoblastoma family of proteins (pRb), which includes pRb/p105, p107 and pRb2/p130, acts as one of the most important regulators of the G1/S transition of the cell cycle. Rb proteins represent an important target for viral oncoproteins. Early viral T antigens can bind all members of the pRb family, promoting the activation of the E2F family of transcription factors, thus inducing the expression of genes required for the entry to the S phase. The interaction between early viral antigens and cell cycle regulators represents an important mechanism through which viruses deregulate cell cycle ...
Transport of α-aminoisobutyric acid and 2-deoxy-d-glucose in African green monkey kidney cells was measured 8 to 100 hr following permissive simian virus 40 infection. No differences in transport were detected during the time-period studies, and no significant differences were seen between the apparent Michaelis-Menten constants of normal and virally infected cells. The absence of transport enhancement in permissive simian virus 40 infection suggests that the augmented transport of viral-transformed cell lines devolves upon altered host genome function.. ...
Alpha-globin transcription factor CP2 is a protein that in humans is encoded by the TFCP2 gene.[1][2] TFCP2 is also called Late SV40 factor (LSF) and it is induced by well known oncogene AEG-1.[3] Late SV40 factor (LSF) also acts as an oncogene in hepatocellular carcinoma.[4] Late SV40 factor (LSF) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9).[5] Along with its main oncogene function in hepatocellular carcinoma (HCC) it plays multifaceted role in chemoresistance, epithelial-mesenchymal transition (EMT), allergic response, inflammation and Alzheimers disease.[4][6] The small molecule FQI1 (factor quinolinone inhibitor 1) prevents LSF from binding to HCC DNA which results in HCC cell death.[4][6][7] ...
COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication. The CV-1 cell line in turn was derived from the kidney of the African green monkey. The acronym COS is derived from the cells being CV-1 (simian) in Origin, and carrying the SV40 genetic material. Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell lines are often used by biologists when studying the monkey virus SV40. Cells from these lines are also often transfected to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. When an expression construct with an SV40 promoter is introduced into COS cells, the vector can be replicated substantially by the large T antigen. These COS cells are genetically modified to produce the T antigen from their own genome. Jensen FC, Girardi AJ, Gilden RV, Koprowski H ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Because of the high homology that exists between the DNA of SV40 and the DNA of the JC or BK viruses, the identity of the band obtained by PCR and visualized by
A family of dihydroquinolinones that inhibited the proliferation of a number of cancer cell lines and targeted the oncogenic activities of the late simian virus 40 factor (LSF) was discovered. The lead quinolinone inhibitors, 8-(2-propoxyphenyl)-7,8-dihydro-[1,3]dioxolo[4,5-g]quinolin-6(5H)-one, FQI1, and 8-(2-propoxyphenyl)-[1,3]dioxolo[4,5-g]quinolin-6(5H)-one, FQI2, were determined by a comprehensive SAR study. The lead compounds had low micromolar to nanomolar Gi50S and IC50S (concentrations that induced 50% inhibition) in cell growth and LSF-directed luciferase reporter assays, respectively. A distinct correlation between the GI50 and IC50 values indicated antiproliferative effects resulted from inhibition of LSF activity. FQI1 had no growth effects on immortalized human hepatocytes or primary mouse hepatocytes. Overall, FQI1 proved a good drug candidate for hepatocellular carcinoma (HCC). It possessed a low molecular weight and moderate solubility, which was improved by substitution of the ...
mouse connexin45(Cx45) genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), mouse phosphoglycerate kinase promoter (PGK promoter), Phage P1 loxP, E. coli neo, E. coli ...
Simian Virus 40 or Simian vacuolating virus 40 - a polyomavirus that is found in both monkeys and humans. As with other polyomaviruses, it is a DNA virus that can cause tumors. ...
washed sheep red bloodand, hereby, warrant that these ingredients are safe for injection into the body of my patient. I have researched reports to the contrary, such as reports that mercury thimerosal causes severe neurological and immunological damage, and find that they are not credible.I am aware that some vaccines have been found to have been contaminated with Simian Virus 40 (SV 40) and that SV 40 is causally linked by some researchers to non-Hodgkins lymphoma and mesotheliomas in humans as well as in experimental animals. I hereby warrant that the vaccines I employ in my practice do not contain SV 40 or any other live viruses. (Alternately, I hereby warrant that said SV-40 virus or other viruses pose no substantive risk to my patient.)I hereby warrant that the vaccines I am recommending for the care of (Patients name) _______________ do not contain any tissue from aborted human babies (also known as fetuses ...
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Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Pk (V5) Epitope Tag (GKPIPNPLLGLDST), 0.1 mg. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Anderson HA, Chen Y, Norkin LC. 1998. MHC class I molecules are enriched in caveolae but do not enter with simian virus 40.. J Gen Virol. 79 ( Pt 6):1469-77. ...
Nuclear targeting sequences are essential for the transport of proteins into the nucleus. The seven-amino-acid nuclear targeting sequence of the SV40 large T antigen has been regarded as the model; however, many nuclear targeting sequences appear to be more complex. We suggest in this review that, d …
include ,EXTERN.h, #include ,perl.h, static PerlInterpreter *my_perl; /** my_eval_sv(code, error_check) ** kinda like eval_sv(), ** but we pop the return value off the stack **/ SV* my_eval_sv(SV *sv, I32 croak_on_error) { dSP; SV* retval; PUSHMARK(SP); eval_sv(sv, G_SCALAR); SPAGAIN; retval = POPs; PUTBACK; if (croak_on_error && SvTRUE(ERRSV)) croak(SvPVx_nolen(ERRSV)); return retval; } /** match(string, pattern) ** ** Used for matches in a scalar context. ** ** Returns 1 if the match was successful; 0 otherwise. **/ I32 match(SV *string, char *pattern) { SV *command = newSV(0), *retval; sv_setpvf(command, my $string = %s; $string =~ %s, SvPV_nolen(string), pattern); retval = my_eval_sv(command, TRUE); SvREFCNT_dec(command); return SvIV(retval); } /** substitute(string, pattern) ** ** Used for =~ operations that modify their left-hand side (s/// and tr///) ** ** Returns the number of successful matches, and ** modifies the input string if there were any. **/ I32 substitute(SV **string, char ...
include ,EXTERN.h, #include ,perl.h, static PerlInterpreter *my_perl; /** my_eval_sv(code, error_check) ** kinda like eval_sv(), ** but we pop the return value off the stack **/ SV* my_eval_sv(SV *sv, I32 croak_on_error) { dSP; SV* retval; STRLEN n_a; PUSHMARK(SP); eval_sv(sv, G_SCALAR); SPAGAIN; retval = POPs; PUTBACK; if (croak_on_error && SvTRUE(ERRSV)) croak(SvPVx(ERRSV, n_a)); return retval; } /** match(string, pattern) ** ** Used for matches in a scalar context. ** ** Returns 1 if the match was successful; 0 otherwise. **/ I32 match(SV *string, char *pattern) { SV *command = NEWSV(1099, 0), *retval; STRLEN n_a; sv_setpvf(command, my $string = %s; $string =~ %s, SvPV(string,n_a), pattern); retval = my_eval_sv(command, TRUE); SvREFCNT_dec(command); return SvIV(retval); } /** substitute(string, pattern) ** ** Used for =~ operations that modify their left-hand side (s/// and tr///) ** ** Returns the number of successful matches, and ** modifies the input string if there were any. **/ I32 ...
This module is the first in a series that discusses the discovery, structure, and function of DNA. Key experiments are discussed: from Griffiths discovery of genetic
Ive been reading a lot about CFIDs and fibro. Some theories are that is has a viral origin, EBV and HHV6 specifically.I have tested postive for both these viruses.....
Latest Side Order of Life review: I have just started watching Side order of life and I loved it. Although I saw just a couple of ...
... is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and ... Barbanti-Brodano, G; Sabbioni, S; Martini, F; Negrini, M; Corallini, A; Tognon, M (2004). "Simian virus 40 infection in humans ... Martini, F; Corallini, A; Balatti, V; Sabbioni, S; Pancaldi, C; Tognon, M (9 July 2007). "Simian virus 40 in humans". ... NIH/National Cancer Institute (2004-08-25). "Studies Find No Evidence That Simian Virus 40 Is Related To Human Cancer". Science ...
Carbone M (1999). "Simian virus 40 and human tumors: It is time to study mechanisms". J Cell Biochem. 76 (2): 189-93. doi: ... which helps prevent infection with wild virus in areas where the virus is endemic. The live virus used in the vaccine is shed ... "Simian Virus 40 (SV40), Polio Vaccine, and Cancer". Vaccine Safety. Centers for Disease Control. 2004-04-22. Archived from the ... Vilchez R, Kozinetz C, Arrington A, Madden C, Butel J (2003). "Simian virus 40 in human cancers". Am J Med. 114 (8): 675-84. ...
Maxwell, SA; Ames, SK; Sawai, ET; Decker, GL; Cook, RG; Butel, JS (February 1991). "Simian virus 40 large T antigen and p53 are ... Vilchez, RA; Kozinetz, CA; Arrington, AS; Madden, CR; Butel, JS (1 June 2003). "Simian virus 40 in human cancers". The American ... Vilchez, RA; Butel, JS (September 2003). "Simian virus 40 and its association with human lymphomas". Current oncology reports. ... "Influence of the viral regulatory region on tumor induction by simian virus 40 in hamsters". Journal of Virology. 82 (2): 871-9 ...
Vilchez, Regis A; Kozinetz, Claudia A; Arrington, Amy S; Madden, Charles R; Butel, Janet S (2003). "Simian virus 40 in human ... Glioblastoma has been associated with the viruses SV40, HHV-6, and cytomegalovirus. No links have been found with the ... with a small fraction of patients under 40 years of age achieving a population-based cure. The population-based cure is thought ... although it has been estimated that only approximately 40% of patients required this treatment. Recently, it has been ...
Aloni Y, Dhar R, Khoury G (October 1979). "Methylation of nuclear simian virus 40 RNAs". Journal of Virology. 32 (1): 52-60. ... Many RNA viruses including SV40, adenovirus, herpes virus, Rous sarcoma virus, and influenza virus have been known to contain ... and Zika virus (ZIKV). These results suggest m6A and its cognate factors play crucial roles in regulating virus life cycle and ... N6-Methyladenosine (m6A) is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including ...
Aloni Y, Dhar R, Khoury G (October 1979). "Methylation of nuclear simian virus 40 RNAs". J. Virol. 32 (1): 52-60. PMC 353526 . ... Beemon K, Keith J (June 1977). "Localization of N6-methyladenosine in the Rous sarcoma virus genome". J. Mol. Biol. 113 (1): ... Kane SE, Beemon K (September 1985). "Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation ... is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants, ...
"BK Virus, JC Virus and Simian Virus 40 Infection in Humans, and Association with Human Tumors". Polyomaviruses and Human ... "The relationship between simian virus 40 and mesothelioma". Current Opinion in Pulmonary Medicine. 14 (4): 316-21. doi:10.1097/ ... The virus causing this illness was isolated in 1937. The rash typical of Lyme borreliosis was identified the early 1900s. ... Natelson, BH; Ye, N; Moul, DE; Jenkins, FJ; Oren, DA; Tapp, WN; Cheng, YC (1994). "High titers of anti-Epstein-Barr virus DNA ...
"Monoclonal antibodies specific for simian virus 40 tumor antigens." Journal of Virology 39.3 (1981): 861-869. Tsai, Li-Huei, et ... Harlow, E (1981). "Monoclonal antibodies specific for simian virus 40 tumor antigens". J Virol. 39: 861-9. PMC 171319 . PMID ... "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product." Science 243.4893 (1989): 934- ... Dyson, N (1989). "The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product". Science. 243 ...
A late protein is a viral protein that is formed after replication of the virus. One example is VP4 from simian virus 40 (SV40 ... "DNA Virus Replication". Daniels R, Sadowicz D, Hebert DN (July 2007). "A very late viral protein triggers the lytic release of ... "Organization of the major and minor capsid proteins in human papillomavirus type 33 virus-like particles". J. Gen. Virol. 76 (9 ...
Turk, B; Porras, A; Mumby, MC; Rundell, K (June 1993). "Simian virus 40 small-t antigen binds two zinc ions". Journal of ... Cheng, Jingwei; DeCaprio, James A.; Fluck, Michele M.; Schaffhausen, Brian S. "Cellular transformation by Simian Virus 40 and ... "Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria". Journal of Virology ... In SV40 and JC virus, STag is not required for viral proliferation, but does improve efficiency. In SV40, STag has a similar ...
Simian virus 40 late polyadenylation signal (SVLPA) Proudfoot, Nick J.; Furger, Andre; Dye, Michael J. (2002). "Integrating ... which in turn recruits the 40S ribosomal subunit. However, a poly(A) tail is not required for the translation of all mRNAs. ... 68 (6): 1336-40. Bibcode:1971PNAS...68.1336E. doi:10.1073/pnas.68.6.1336. Danckwardt, Sven; Hentze, Matthias W; Kulozik, ...
Kelley WL, Georgopoulos C (April 1997). "The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can ... Most of these viruses, such as BK virus and JC virus, are very common and typically asymptomatic in most human populations ... most notably BK virus, JC virus, and SV40. It is essential for proliferation in the viruses that express it and is thought to ... A fourteenths virus has been desciribed. Lyon IARC polyoma virus is related to raccoon polyoma virus. The following 13 ...
"Cellular transformation by Simian Virus 40 and Murine Polyoma Virus T antigens". Seminars in Cancer Biology. 19 (4): 218-228. ... "Polyoma virus middle T is essential for virus replication and persistence as well as for tumor induction in mice". Virology. ... However, evidence of MTag encoding and expression has also recently been reported in at least one virus of unrelated lineage, ... doi:10.1016/0042-6822(92)90247-M. Courtneidge, Sara A.; Smith, Alan E. (2 June 1983). "Polyoma virus transforming protein ...
1988). "SSA/Ro antigen expression in simian virus 40-transformed human keratinocytes". J. Invest. Dermatol. 90 (3): 342-345. ...
"Biochemical Procedure for Production of Small Deletions in Simian Virus 40 DNA." Proccedings of the National Academy of ...
Paul D, Lipton A, Klinger I (1971). "Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts ...
Later, Daniel Nathans and Kathleen Danna showed that cleavage of simian virus 40 (SV40) DNA by restriction enzymes yields ... Danna K, Nathans D (Dec 1971). "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae ... There is research on REases and ZFN that can cleave the DNA of various human viruses, including HSV-2, high-risk HPVs and HIV-1 ... The term restriction enzyme originated from the studies of phage λ, a virus that infects bacteria, and the phenomenon of host- ...
"Interactions of early mouse embryos with oncogenic viruses-Simian virus 40 and polyoma. I. Ultrastructural studies." Journal ... "Virus particles in early mouse embryos." Journal National Cancer Institute 51 (1973): 1041-1050. Print. Lewandowski, L.J., F.S ... "Analysis of a viral agent isolated from multiple sclerosis (MS) brain tissue: Characterization as a parainfluenza virus type I ... They were cultured." At Wistar, Pienkowski collaborated with Koprowski on research into cancer-causing viruses. Upon returning ...
"Studies Find No Evidence That Simian Virus 40 Is Related To Human Cancer." ScienceDaily. ScienceDaily, 25 August 2004. . SV40 ... Rogue virus in the vaccine: Early polio vaccine harbored virus now feared to cause cancer in humans, William Carlsen, San ... Martin's Press, 2004, ISBN 0-312-27872-1 The Virus and the Vaccine official website "The Virus and the Vaccine", Debbie ... SV40 stands for Simian Virus 40' official website website in memory of Alexander Horwin. ...
Gruss P, Dhar R, Khoury G (February 1981). "Simian virus 40 tandem repeated sequences as an element of the early promoter". ... 159 (6): 1327-40. doi:10.1016/j.cell.2014.11.023. PMC 4364385 . PMID 25480297. Sun J, Rockowitz S, Xie Q, Ashery-Padan R, Zheng ...
Klietmann, W.; Sato, N; Nass, MM (1973). "ESTABLISHMENT AND CHARACTERIZATION OF ETHIDIUM BROMIDE RESISTANCE IN SIMIAN VIRUS 40- ... Klietmann, W; Sato, N; Nass, MM (1973). "Establishment and characterization of ethidium bromide resistance in simian virus 40- ... For five years, Klietmann headed a research group at the Max-Planck-Institute for Virus Research in Tübingen (Germany), and ... Sato, N; Chance, B; Kato, K; Klietmann, W (1973). "Comparative study of cytochromes between virus-transformed and untransformed ...
BK virus or Simian virus. The Ebola virus may also be found in urine from an infected person. The exact survival time of this ... Vanchiere, John A. (12 January 2005). "Detection of BK virus and simian virus 40 in the urine of healthy children". Journal of ... "Ebola (Ebola Virus Disease)Transmission, Q&As on Transmission". CDC (Centres for Disease Control and Prevention). 20 November ... particular virus in human urine outside of the human body is unclear but probably "up to several days" like with other body ...
"Enumeration of the simian virus 40 early region elements necessary for human cell transformation." Mol Cell Biol 22 (7): 2111- ... from homeobox genes to DNA tumor viruses and human antibody therapeutics. His technique for cloning native human antibodies has ...
Yamamoto H, Shimojo H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ... Once the virus has successfully gained entry into the host cell, the endosome acidifies, which alters virus topology by causing ... Although this is significantly larger than other viruses in its Baltimore group, it is still a very simple virus and is heavily ... Wu E, Nemerow GR (2004). "Virus yoga: the role of flexibility in virus host cell recognition". Trends Microbiol. 12 (4): 162- ...
"Spliced and unspliced virus specific RNA sequences are associated with purified simian virus 40 chromatin". Nucleic Acids ... "Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts". Nature. 323 (6088): 544-8. doi: ... Section DNA Tumor Viruses (Gerhard Sauer) for doctoral studies. He received his PhD from Ruprecht-Karls-University, Heidelberg ...
"Identification of the oncogenic substance in rhesus monkey kidney cell culture as simian virus 40". Virology. 17 (1): 65-75. ... The virus was named the Stewart-Eddy or SE polyoma virus, after their respective surnames. In 1954, while the NIH was testing ... the discovery explained the origins of the widespread contamination of a variety of stocks of seed viruses and live polio virus ... Levine, A.J. The Origins of Small DNA Tumor Viruses In: Foundations in Cancer Research, Academic Press, 1994 pp. 152-153. ...
Clever, J.; Dean, D. A.; Kasamatsu, H. (1993-10-05). "Identification of a DNA binding domain in simian virus 40 capsid proteins ... Gasparovic, M. L.; Gee, G. V.; Atwood, W. J. (2006-11-01). "JC Virus Minor Capsid Proteins Vp2 and Vp3 Are Essential for Virus ... "The VP2/VP3 Minor Capsid Protein of Simian Virus 40 Promotes the in Vitro Assembly of the Major Capsid Protein VP1 into ... "Minor Capsid Proteins of Simian Virus 40 Are Dispensable for Nucleocapsid Assembly and Cell Entry but Are Required for Nuclear ...
... (Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from ... Chen S, Paucha E (July 1990). "Identification of a region of simian virus 40 large T antigen required for cell transformation ... Encodes non-structural proteins (i.e. not present in virus particle). Late: Expressed later in virus infection, i.e. during and ... Sweet BH, Hilleman MR (November 1960). "The vacuolating virus, S.V. 40". Proc. Soc. Exp. Biol. Med. 105 (2): 420-427. doi: ...
Cheng, Jingwei; DeCaprio, James A.; Fluck, Michele M.; Schaffhausen, Brian S. "Cellular transformation by Simian Virus 40 and ... although the exact molecular mechanisms vary from one virus to another. The SV40 large T antigen from the SV40 virus is the ... LTag has two primary functions, both related to replication of the viral genome: it unwinds the virus's DNA to prepare it for ... Some polyomavirus LTag proteins - most notably the well-studied SV40 large tumor antigen from the SV40 virus - are oncoproteins ...
Kelly, T J; Rose, J A (1971). "Simian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule". ... "The DNA-activated protein kinase is required for the phosphorylation of replication protein A during simian virus 40 DNA ... Kelly pioneered the study of DNA replication in eukaryotic cells by using DNA viruses as model systems. His laboratory ... where he began to exploit viruses as potentially powerful model systems for exploring the mechanisms of DNA replication in ...
This lab outbreak was initially diagnosed as simian haemorrhagic fever virus (SHFV) and occurred amongst a shipment of crab- ... The four are Bundibugyo virus (BDBV), Sudan virus (SUDV), Taï Forest virus (TAFV) and one simply called Ebola virus (EBOV, ... The virus responsible for the initial outbreak, first thought to be Marburg virus, was later identified as a new type of virus ... Main articles: Ebola virus cases in the United States, Ebola virus disease in Spain, and Ebola virus disease in the United ...
"Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing ... 40] en 1987, D. T. Burke et al. describen o uso de cromosomas artificiais de lévedo (YAC, Yeast Artificial Chromosome),[41] e ...
"Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". ... Molekul DNA rekombinan pertama dibuat oleh Paul Berg pada tahun 1972 dengan menggabungkan DNA virus monyet SV40 dengan virus ... "Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing ... Paul Berg menciptakan molekul DNA rekombinan pertama dengan menggabungkan DNA dari virus monyet SV40 dengan virus lambda.[22] ...
... passage of simian immunodeficiency virus by unsterile injections and the emergence of epidemic human immunodeficiency virus in ... O grupo de Montagnier denominou o seu novo virus illado virus asociado á linfadenopatía (LAV).[181] Como estes dous virus se ... Despois de que o virus entra no corpo hai un período de rápida replicación viral, que fai que o virus sexa abondoso no sangue ... O grupo de Gallo chamou o seu novo virus illado HTLV-III. Ao mesmo tempo, o grupo de Montagnier illou un virus dun paciente que ...
It is a member of the Semliki Forest virus complex and is closely related to Ross River virus, O'nyong'nyong virus, and Semliki ... and false positives can occur with infection due to other related viruses, such as o'nyong'nyong virus and Semliki Forest virus ... Chikungunya virus is passed to humans when a bite from an infected mosquito breaks the skin and introduces the virus into the ... This mutation potentially allows the virus to multiply more easily in mosquito cells.[74] The change allows the virus to use ...
Carbone M (1999). "Simian virus 40 and human tumors: It is time to study mechanisms". J Cell Biochem. 76 (2): 189-93. PMID ... "Simian Virus 40 (SV40), Polio Vaccine, and Cancer". Vaccine Safety. Centers for Disease Control. 2004-04-22. Retrieved 2010-12- ... Vilchez R, Kozinetz C, Arrington A, Madden C, Butel J (2003). "Simian virus 40 in human cancers". Am J Med. 114 (8): 675-84. ... "Cancer risk associated with receipt of vaccines contaminated with simian virus 40: epidemiologic research" (PDF). Expert Rev ...
Jackson, D. A., Symons, R. H., Berg, P. Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: ... Jaenisch, R., Mintz, B. Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts ... New virus-built battery could power cars, electronic devices [online]. Web.mit.edu, 2009-04-02 [cit. 2010-07-17]. Dostupné ... Hidden Ingredient In New, Greener Battery: A Virus [online]. Npr.org [cit. 2010-07-17]. Dostupné online. (anglicky). Je zde ...
... and Simian virus 40 in 1976 and 1978, respectively.[14][15] ... Viruses and bacteriophagesEdit. Bacteriophages have played and ... "Complete genomes: Viruses". NCBI. 2011-11-17. Retrieved 2011-11-18.. *^ "Genome Project Statistics". Entrez Genome Project. ... 2,719 viruses, 1,115 archaea and bacteria, and 36 eukaryotes, of which about half are fungi.[29][30] ... While the growth in the use of the term has led some scientists (Jonathan Eisen, among others[40]) to claim that it has been ...
Jackson, RJ; DJ Maguire; LA Hinds; IA Ramshaw (1998). "Infertility in mice induced by a recombinant ectromelia virus expressing ... development of a simian model for testing of anti-FA-1 contraceptive vaccine". J Reprod Immunol. 27: 111-121. doi:10.1016/0165- ... Research in these countries has therefore focused on genetically modifying viruses or microorganisms that infect the unwanted ... in Australia by engineering rabbit zona pellucida glycoproteins into a recombinant myxoma virus. This approach has induced ...
... virus called the "human respiratory virus-27". The study used a fake virus to prevent participants from having thoughts, ... The resulting choices of the human and simian subjects concorded with the theory of cognitive dissonance when the children and ... The study participants were in two groups; one group was told that they were actual candidates for the virus-27 test, and the ... opinions, and feeling about the virus that would interfere with the experiment. ...
1972). Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules ...
Famili Polyomaviridae - termasuk Simian virus 40, virus JC. *Famili Poxviridae - termasuk Cowpox virus, smallpox ... Virus DNA adalah virus yang masuk pada baik Grup I atau Grup II pada sistem klasifikasi Baltimore terhadap virus. Virus ini ... "ICTVdb Index of Viruses: Virus Taxonomy, 8th Reports of the International Committee on Taxonomy of Viruses: Listing in ... Artikel bertopik virus ini adalah sebuah rintisan. Anda dapat membantu Wikipedia dengan mengembangkannya.. ...
"Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing ... biokütus - vähendatakse biokütuse tootmiskulusid.[40]. Geenmuundatud kultuurid[muuda , muuda lähteteksti]. GM-kultuuride ... Atlandi lõhe 40 000 geenist lisati harilikult Chinooki lõhes kasvav hormoonide regulatoorne geen ja Zoarces americanus'e ... "Development of genetically engineered resistant papaya for papaya ringspot virus in a timely manner: a comprehensive and ...
"Expression of enhanced levels of small RNA polymerase III transcripts encoded by the B2 repeats in simian virus 40-transformed ... 40 (2): 158-60. doi:10.1038/ng.2007.55. PMC 2647775 . PMID 18176564. Pibouin L, Villaudy J, Ferbus D, et al. (February 2002). " ... One ncRNA, termed HOTAIR, that originates from the HOXC locus represses transcription across 40 kb of the HOXD locus by ... tiling array data shows more than 40% of transcripts are non-polyadenylated). However, unambiguously identifying ncRNAs within ...
"Specific Cleavage of Simian Virus 40 DNA by Restriction Endonuclease of Hemophilus Influenzae". Proc. Natl. Acad. Sci. U.S.A. ... StuI[40][41]. Streptomyces tubercidicus 5'AGGCCT 3'TCCGGA 5'---AGG CCT---3' 3'---TCC GGA---5' ...
Ang mga ito ang henetikong impormasyon na nakokodigo sa asidong nukleyiko(DNA, o RNA para sa mga virus), naka-transkriba sa RNA ... Ang natitirang Haplorhini na Simian ay naghiwalay sa mga pangkat na Catarrhini at Platyrrhini. Ang linyang Platyrrihni(Bagong ... Ang mga Endogenous retrovirus (ERV) ang mga labing sekwensiya ng genome na naiwan mula sa mga sinaunang impeksiyong pang-virus ... Ang mga Virus ay nagdadala rin ng DNA sa pagitan ng mga organismo na pumapayag sa paglilipat ng mga gene sa ibayong mga dominyo ...
Another metallo-porphyrin complex, the iron chelate, is more photoactive (towards HIV and simian immunodeficiency virus in MT-4 ... fungi and viruses.[1] PDT is popularly used in treating acne. It is used clinically to treat a wide range of medical conditions ... Photosensitisers have been employed to sterilise blood plasma and water in order to remove blood-borne viruses and microbes and ... In the 1980s, David Dolphin, Julia Levy and colleagues developed a novel photosensitizer, verteporfin.[39][40] Verteporfin, a ...
Populations Exposed To Simian Immunodeficiency Viruses". ScienceDaily. Retrieved 2009-08-15.. ... extinct non-hominoid simians, including early Catarrhines are discussed as monkeys as well as simians or anthropoids,[32][33][ ... Infraorder Simiiformes: simians *Parvorder Platyrrhini: New World monkeys *Family Callitrichidae: marmosets and tamarins (42 ... Extinct basal simians such as Aegyptopithecus or Parapithecus [35-32 million years ago], eosimiidea and sometimes even the ...
"Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". ... "Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing ... Paul Berg created the first recombinant DNA molecules by combining DNA from the monkey virus SV40 with that of the lambda virus ... Fungal and virus resistant crops have also been developed or are in development.[125][126] This makes the insect and weed ...
Multiplicity reactivation has been found to occur with pathogenic viruses including influenza virus, HIV-1, adenovirus simian ... Viruses[edit]. Viruses are capsid-encoding organisms composed of proteins and nucleic acids that can self-assemble after ... Many types of virus are capable of genetic recombination. When two or more individual viruses of the same type infect a cell, ... When two or more viruses, each containing lethal genomic damage infect the same host cell, the virus genomes often can pair ...
"Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing ... As of 2005, about 13% of the Zucchini (a form of squash) grown in the US was genetically modified to resist three viruses; that ... Papaya was genetically modified to resist the ringspot virus (PSRV). "SunUp" is a transgenic red-fleshed Sunset papaya cultivar ... Its single-handed savior was a breed engineered to be resistant to the virus. Without it, the state's papaya industry would ...
2005). "Central African hunters exposed to simian immunodeficiency virus". Emerg Infect Dis 11 (12): 1928-30. doi:10.3201/ ... passage of simian immunodeficiency virus by unsterile injections and the emergence of epidemic human immunodeficiency virus in ... 37.0 37.1 Reeves, J. D. and Doms, R. W (2002). "Human Immunodeficiency Virus Type 2". J. Gen. Virol. 83 (Pt 6): 1253-65. doi: ... Laurence J (2006). "Hepatitis A and B virus immunization in HIV-infected persons". AIDS Reader 16 (1): 15-17. PMID 16433468 . ...
Sato, S (February 1978). "A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus ...
Epstein-Barr virus (EBV) infection is associated with nasopharyngeal cancer. Nasopharyngeal cancer occurs endemically in some ... and 8-oxo-purines in simian kidney cells". Nucleic Acids Res. 34 (8): 2305-15. doi:10.1093/nar/gkl099. PMC 1458282 . PMID ... Distinct host genome methylation and expression patterns, produced even when virus isn't integrated into the host genome. ... and Epstein-Barr virus. Head and neck cancers are most commonly of the squamous cell carcinoma type. The diagnosis is confirmed ...
"Expression cloning of new receptors used by simian and human immunodeficiency viruses.". Nature 388 (6639): 296-300. PMID ... 2003). "Expression of chemokines and their receptors in human and simian astrocytes: evidence for a central role of TNF alpha ... orphan seven-transmembrane segment receptors which are expressed in CD4-positive cells support simian immunodeficiency virus ... "Gp120-induced Bob/GPR15 activation: a possible cause of human immunodeficiency virus enteropathy.". Am. J. Pathol. 159 (5): ...
... bunchy top virus. Banana bunchy top virus (BBTV) is a plant virus of the genus Babuvirus, family Nanonviridae affecting ... "Comparing Black People to Monkeys has a Long, Dark Simian History". Huffington Post. Archived from the original on January 3, ... Infected plants may produce no fruit or the bunch may not emerge from the pseudostem.[90] The virus is transmitted by the ... There is no cure for BBTD, but it can be effectively controlled by the eradication of diseased plants and the use of virus-free ...
Urban performing macaques also carried simian foamy virus, suggesting they could be involved in the species-to-species jump of ... Nearly all (73-100%) pet and captive rhesus macaques are carriers of the herpes B virus. This virus is harmless to macaques, ... University of Toronto - [email protected] - Performing monkeys in Asia carry viruses that could jump species to humans (Dec 8/05) ... "B-virus from Pet Macaque Monkeys: An Emerging Threat in the United States?". Emerging Infectious Diseases. 4 (1): 117-121. doi ...
The simian virus 40 late polyadenylation signal (SVLPA) is an RNA element which promotes efficient polyadenylation. Wu, C.; ... Page for Simian virus 40 late polyadenylation signal (SVLPA) at Rfam. ...
SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and ... Barbanti-Brodano, G; Sabbioni, S; Martini, F; Negrini, M; Corallini, A; Tognon, M (2004). "Simian virus 40 infection in humans ... Martini, F; Corallini, A; Balatti, V; Sabbioni, S; Pancaldi, C; Tognon, M (9 July 2007). "Simian virus 40 in humans". ... NIH/National Cancer Institute (2004-08-25). "Studies Find No Evidence That Simian Virus 40 Is Related To Human Cancer". Science ...
Müller K, Mermod N (2000). "The histone-interacting domain of nuclear factor I activates simian virus 40 DNA replication in ... Ravichandran V, Sabath BF, Jensen PN, Houff SA, Major EO (2006). "Interactions between c-Jun, nuclear factor 1, and JC virus ... NFI-X3, a splice variant of NFIX, regulates Glial fibrillary acidic protein and YKL-40 in astrocytes. NFIX has been shown to ... "A complex of nuclear factor I-X3 and STAT3 regulates astrocyte and glioma migration through the secreted glycoprotein YKL-40". ...
Highly radioactive virus probes (SV40-3H-cRNA synthesized in a cell-free system and the separated early and late strands of ... each probe hybridized at saturation levels and the various reaction kinetics indicated that ssDNA is greatly enriched for virus ... Relationship Between Single-stranded DNA Isolated from Mouse Cells Transformed by Simian Virus 40 and Transcription of Cellular ... ICTV Virus Taxonomy Profile: Flaviviridae Peter Simmonds, Paul Becher, Jens Bukh, Ernest A. Gould, Gregor Meyers, Tom Monath, ...
Absence of simian virus 40 (SV40) DNA in lymphoma samples from Tasmania, Australia ... Absence of simian virus 40 (SV40) DNA in lymphoma samples from Tasmania, Australia. You are here *UTAS Home ... Sui, LF and Williamson, J and Lowenthal, RM and Parker, AJC, Absence of simian virus 40 (SV40) DNA in lymphoma samples from ... may have been caused by human infection with simian virus 40 (SV40) (a lymphotropic monkey virus that was introduced to man ...
Simian virus 40 DNA replication in vitro : Identification of multiple stages of initiation. / Tsurimoto, T.; Fairman, M. P.; ... Tsurimoto T, Fairman MP, Stillman B. Simian virus 40 DNA replication in vitro: Identification of multiple stages of initiation ... Tsurimoto, T. ; Fairman, M. P. ; Stillman, B. / Simian virus 40 DNA replication in vitro : Identification of multiple stages of ... Tsurimoto, T, Fairman, MP & Stillman, B 1989, Simian virus 40 DNA replication in vitro: Identification of multiple stages of ...
A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro. scientific article ... A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro. (English) ... Enhanced transformation by a simian virus 40 recombinant virus containing a Harvey murine sarcoma virus long terminal repeat ...
Choi, S. K., Lee, S. H., & Chang, H. I. (1998). Changes of Nuclear Glycoproteins in Normal and Simian Virus 40-Transformed WI- ... Changes of Nuclear Glycoproteins in Normal and Simian Virus 40-Transformed WI-38 Human Lung Fibroblasts. Molecules and cells. ... Fingerprint Dive into the research topics of Changes of Nuclear Glycoproteins in Normal and Simian Virus 40-Transformed WI-38 ... Changes of Nuclear Glycoproteins in Normal and Simian Virus 40-Transformed WI-38 Human Lung Fibroblasts. / Choi, Seok Keun; Lee ...
Simian Virus 40 (SV40):. A Cancer Causing Monkey Virus from FDA-Approved Vaccines. The Creation and Production of the Polio ...
A Colinear Map Relating the Simian Virus 40 (SV40) DNA Segments of Six Adenovirus-SV40 Hybrids to the DNA Fragments Produced by ... Specific Cleavage of Simian Virus 40 DNA by Restriction Endonuclease of Hemophilus Influenzae ...
Separation and properties of two kinds of simian virus 40 late transcription complexes. Llopis R, Stark GR. Llopis R, et al. J ... Viral proteins and RNAs in BHK cells persistently infected by lymphocytic choriomeningitis virus. van der Zeijst BA, Bleumink N ... Persistent infection of some standard cell lines by lymphocytic choriomeningitis virus: transmission of infection by an ...
Simian virus 40 early promoter (SV40 early). Escherichia coli lac operon promoter. ...
Simian virus 40 bidirectional origin (SV40). Phage f1 origin. Host range:. Escherichia coli. Mammalian cells; SV40 permissive ... Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA). Selection marker:. Neomycin (neo; G418; ...
Simian virus 40 polyadenylation signal (SV40 polyA). Selection marker:. Ampicillin (amp). Replicon:. Escherichia coli plasmid ...
The first link was titled "Cancer, Simian Virus 40 (SV40), and Polio Vaccine Fact Sheet" and was dated 22 October 2007. If it ... The CDC quietly removed pages from their web site that admitted a link between Simian Virus 40 (SV40), the polio vaccine, and ... The second link was titled "Frequently Asked Questions about Cancer, Simian Virus 40 (SV40), and Polio Vaccine" and contained ... o Sometime around 1960, Simian Virus 40 (SV40) was discovered in the injected form of the polio vaccine (IPV). ...
Simian virus 40 not associated with non-Hodgkin lymphoma, study shows. Post Your Comments: *Name:. ...
Simian virus 40, asbestos and malignant mesothelioma Fang Qi, 2016. University of Hawaii - Manoa, #2135267 posted on 02/05/2017 ...
... in which the simian virus 40 promoters have been replaced by Rous sarcoma virus (RSV) promoters. They express fusion proteins ... Cells were untreated or treated with BP (2.5 μM) for 40 h. In addition, cell were treated with catalase (100 U/ml) (lanes 5 to ... for 40 h. (B) Effect of oxidative stress on the TADs of NFI/CTF, Sp1, and Oct. Cells were treated or not with H2O2 (17.5 μM) ... 40). In the case of TNF-α, we have shown that NFI and ROS were involved in the signaling mechanism (37). In an interesting ...
BMP9 Can Induce Schwann Cells Expressing Simian Virus 40 T Antigen to Differentiate into Fat and Bone and .. Authors:. Rui-Fang ... In our previous study, we constructed Schwann cells (SCs) that stably express Simian virus 40 T antigen (SV40T-SCs). SV40T-SCs ...
Simian virus 40 * Osteopontin * Fibroblast Growth Factor 2 * Transforming Growth Factors * Capillaries ...
Transcription of simian virus 40. III : mapping of "early" and "late" species of RNA. Proceedings of the National Academy of ...
SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter ... To quantify Golgi-associated Arf-1-GFP, a 40× 0.7 NA objective was used with a pinhole corresponding to a focal depth of 12.4 ... and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. ... using a 40× 0.7 NA objective with a pinhole corresponding to a focal depth of 2.5 μm. Image processing for figures was ...
... of DNA polymerases at the eukaryotic replication fork was possible after the in vitro reconstitution of the simian virus 40 ( ... of DNA polymerases at the eukaryotic replication fork was possible after the in vitro reconstitution of the simian virus 40 ( ... of DNA polymerases at the eukaryotic replication fork was possible after the in vitro reconstitution of the simian virus 40 ( ... of DNA polymerases at the eukaryotic replication fork was possible after the in vitro reconstitution of the simian virus 40 ( ...
Simian virus 40 large T antigen Capable of inducing mlignant transformation of a variety of cell types; can be used to ... Simian diphtheria toxin receptor Diphtheria toxin (DT) Cells expressing DTR are sensitive to diphtheria toxin (DT); DTR can be ...
Simian virus 40 large T antigen Guide View Related Vectors hHRAS. 513 bp. Homo sapiens Ras proto-oncogene. Belongs to the Ras ...
See the Simian Virus 40 research here." (source). 3. Mounting Evidence Proves Vaccines Dont Work As Promoted. The belief that ... There is also the case of the simian virus known as SV-40 which contaminated the original oral polio vaccines, and which is ... Contains beneficial strains of yeast and bacteria to ward off viruses, bacteria and other harmful organisms.. 5. Grass fed bone ... "Another problem is the use of euphemisms like weakened or attenuated to describe live-virus containing vaccines, implying ...
  • The genome of Simian Virus 40 (SV40) is a covalently closed circular DNA duplex with about 5200 nucleotide pairs. (springer.com)
  • Simian virus 40 (SV40)-mediated transformation of human diploid fibroblasts has provided an effective experimental system for studies of both "senescence" in cell culture and carcinogenesis. (asm.org)
  • We have analysed nucleotide sequences at the junction between simian virus 40 (SV40) and cellular DNA in the Fisher rat transformed line tsA30-N2. (pasteur.fr)
  • Domains of simian virus 40 large t-antigen exposed on the cell surface. (jax.org)
  • These two positions are located within short direct repeats in the virus genome. (pasteur.fr)
  • Our analysis demonstrates that in both cases, recombination preferentially takes place near short direct repeats in the virus genome. (pasteur.fr)
  • Anti-biotics kill the beneficial bacteria in the digestive tract and this allows the polio virus to enter the body through the intestines. (larrysmusings.com)
  • This past weekend, we heard of reports that the CDC recently had for a very short time on its website a news item that as many as 98 million Americans vaccinated for polio had been exposed to SV-40 (simian virus 40). (larrysmusings.com)
  • Even me, not a doctor, was aware since early 2000 that polio vaccinations between 1955 and 1963 were tainted with this simian virus (from monkeys). (larrysmusings.com)
  • Immortalization of human fibroblasts transformed by origin-defective simian virus 40. (asm.org)
  • But, tainted vaccines were knowingly distributed throughout the country because the doctors believed at the time that animal viruses posed no health threat to humans, that the virus that harms monkeys could not harm humans. (larrysmusings.com)
  • So, humans are not immune to all viruses and all bacteria that also attack and harm animals. (larrysmusings.com)
  • Previous interpretations may have been complicated, however, by the semipermissive virus-cell interaction. (asm.org)
  • This virus is adept at fooling the human immune system and thus is dangerous to a human's health. (larrysmusings.com)
  • Since, the estimates range widely from 30 to 98 million people being exposed to SV 40, the reality is that there will, over time, be more cases of cancer than if they had not been exposed to this animal virus. (larrysmusings.com)
  • You see, the tonsils are the only organ of the body that is capable of manufacturing anti-bodies to the poli omyelitis virus. (larrysmusings.com)
  • Genomic sequences of the human polyomaviruses, JC virus (JCV) and BK virus (BKV), and simian virus 40 (SV40) have been reported from several types of human brain tumors, but there have been no population-based seroepidemiologic studies to evaluate the association between polyomavirus infection and brain tumors. (aacrjournals.org)
  • The polyomavirus family includes two human viruses, JCV 3 and BKV. (aacrjournals.org)
  • Many investigators detected the simian polyomavirus SV40 footprints in human brain tumors and neurologic diseases and recently it has been indicated that SV40 seems to be associated with multiple sclerosis (MS) disease. (eurekamag.com)
  • Some laboratories have detected DNA from the macaque polyomavirus simian virus 40 (SV40) in human tumors, but possible routes of infection remain unknown. (elsevier.com)
  • SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and humans. (wikipedia.org)
  • Currently, viruses in this family that infect humans are related to the lymphotropic polyomavirus (LPV). (cdc.gov)
  • During the past 4 years, polymerase chain reaction (PCR) assays have detected DNA sequences related to SV40 (an oncogenic simian polyomavirus) in a variety of human tissues, especially choroid plexus tumors, ependymomas, mesotheliomas, and osteosarcomas ( 1 - 7 ). (cdc.gov)
  • Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. (bvsalud.org)
  • Currently, there are twelve members of the genus Polyomavirus recognized by the International Committee on Taxonomy of Viruses (ICTV). (bioscience.org)
  • The six best-characterized members of the group are BK virus (BKV), JC virus (JCV), simian virus 40 (SV40), murine polyomavirus (PyV), hamster papovavirus (HaPV), and lymphotropic papovavirus (LPV). (bioscience.org)
  • The other six polyomaviruses that are recognized by the ICTV include budgerigar fledgling disease virus (BFDV), bovine polyomavirus (BPyV), another murine polyomavirus [Kilham virus (KV)], baboon polyomavirus 2 (PPV-2), rabbit kidney vacuolating virus (RKV), and simian virus agent 12 (SA12). (bioscience.org)
  • In contrast, studies of polyomavirus KV have been based on a single virus isolate. (bioscience.org)
  • Cellular transformation by Simian Virus 40 and Murine Polyoma Virus T antigens. (nih.gov)
  • In this study, we identify an integrated gene signature from multiple transgenic models of epithelial cancers intrinsic to the functions of the Simian virus 40 T/t-antigens that is associated with the biological behavior and prognosis for several human epithelial tumors. (greenmedinfo.com)
  • BACKGROUND: DNA sequences and immunoreactivity associated with Simian virus 40 transforming factors, large T and small t antigens (SV40LTAg), suggestive of an aetiopathogenetic link have been identified in fresh frozen tissue of a high proportion of recent cases of pleural mesotheliomas from the United States, Italy and Germany. (bmj.com)
  • Wild-type and J domain mutant simian virus 40 large T antigens alter the cell cycle and bud morphology of Saccharomyces cerevisiae. (mysciencework.com)
  • Large T antigens play a role in regulating the viral life cycle of the polyomaviridae viruses, such as SV40. (sciencephoto.com)
  • For example, the immune system's response to antigens that are part of bacteria and viruses helps people resist infections. (mesothelioma-line.com)
  • abstract = "Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. (elsevier.com)
  • abstract = "Background: Poliovirus vaccines that were used during the late 1950s and early 1960s were contaminated with simian virus 40 (SV40), a monkey virus that is tumorigenic in rodents. (elsevier.com)
  • Simian virus 40 small T antigen activates AMPK and triggers autophagy to protect cancer cells from nutrient deprivation. (semanticscholar.org)
  • The simian virus 40 (SV40) large-T antigen is essential for SV40 DNA replication and for late viral gene expression, but the role of the SV40 small-t antigen in these processes is still unclear. (meta.org)
  • Fu, XY, Colgan, JD & Manley, JL 1988, ' Multiple cis-acting sequence elements are required for efficient splicing of simian virus 40 small-t antigen pre-mRNA ', Molecular and cellular biology , vol. 8, no. 9, pp. 3582-3590. (elsevier.com)
  • Deletion mutations in the small t antigen gene alter the tissue specificity of tumors induced by simian virus 40. (core.ac.uk)
  • Serum antibodies to JC virus, BK virus, simian virus 40, and the risk of incident adult astrocytic brain tumors. (greenmedinfo.com)
  • Simian virus 40 is found at far higher rates in breast tumors and probably contributes to pathogenesis in those tumors. (greenmedinfo.com)
  • Recently, simian virus 40 (SV40) has been associated with human mesothelioma and brain and bone tumors (reviewed in refs. (pnas.org)
  • The study of DNA tumors viruses has been an extremely important tool in understanding the cellular pathways that regulate cell division and are disrupted in cancer. (bio-medicine.org)
  • Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors in animals, but most often persists as a latent infection. (wikipedia.org)
  • Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. (mesothelioma-line.com)
  • Early versions of the polio vaccine were found to be contaminated with SV40, which led to widespread alarm when tests showed that hamsters infected with the virus grew cancerous tumors. (historyofvaccines.org)
  • Fingerprint Dive into the research topics of 'Simian virus 40 DNA replication: functional organization of regulatory elements. (ntnu.edu.tw)
  • Identification of Simian virus 40 promoter DNA sequences capable of conferring restriction endonuclease hypersensitivity. (asm.org)
  • The simian virus 40 (SV40) DNA sequences found in the enhancer domain, nucleotides (nt) 103 to 177, and the early domain, nt 5149 to 5232, of the SV40 promoter have been analyzed for their ability to confer restriction endonuclease hypersensitivity in SV40 chromatin by using an SV40-based recombinant reporter system. (asm.org)
  • protein) complexes from simian-virus-40 (SV40)-infected cells late in infection contain 7-10% RNA sequences specific to SV40 DNA. (pasteur.fr)
  • We have analysed nucleotide sequences at the junction between simian virus 40 (SV40) and cellular DNA in the Fisher rat transformed line tsA30-N2. (pasteur.fr)
  • It contains an insertion of simian virus 40 (SV40) sequences 3.2 × 103 bases long in place of the 3.5 × 103 base segment of adenovirus 2 DNA which maps between 0.64 and 0.74 fractional genome lengths from the left end of the viral DNA. (cshl.edu)
  • researchers noted the ability of the PCR primers used in these assays to amplify DNA sequences from JC and BK viruses as well as from SV40 and discussed whether each set of primers in the PCR reaction requires specific conditions to amplify virus-specific DNA. (cdc.gov)
  • The DNA tumor virus simian virus 40 produces the Large T antigen which inactivates two of the cell's most important cancer-preventing proteins, p53 and pRb. (bio-medicine.org)
  • The study of the transforming proteins derived from DNA tumor viruses in experimental models of transformation has provided fundamental insights into the process of cell transformation. (semanticscholar.org)
  • The replication of simian virus 40 (SV40) DNA is dependent upon a single viral protein [tumor (T) antigen] and multiple cellular proteins. (duke.edu)
  • Simian virus 40 late proteins possess lytic properties that render them capable of permeabilizing cellular membranes. (umass.edu)
  • The data suggest that the pool of proteins required for maturation is sufficient to convert one-third of the simian virus 40 DNA molecules labeled in a 10-min pulse (at 33 h postinfection) from chromatin to previrions and virions and is exhausted within 1 h. (elsevier.com)
  • A comparison of the proteins synthesized in human cells at late times after infection with adenovirus (Ad2) and with the adeno-simian virus 40 (SV40) hybrid viruses revealed polypeptides of 30,000 and 92,000 molecular weight specific for the hybrid viruses Ad2+ND1 and Ad2+ND4, respectively. (utah.edu)
  • In this study, we attempted the artificial regulation of cell proliferation by protein transduction of the N-terminal domain (1-132 amino acids) of the simian virus 40 large T-antigen (SVLT-N), which inactivates retinoblastoma family proteins but not p53. (elsevier.com)
  • 000114841 001__ 114841 000114841 005__ 20181203021031.0 000114841 037__ $$aARTICLE 000114841 245__ $$aCharacterization of a new tissue-specific transcription factor binding to the simian virus 40 enhancer TC-II (NF-kappa B) element 000114841 269__ $$a1992 000114841 260__ $$c1992 000114841 336__ $$aJournal Articles 000114841 500__ $$aSwiss Institute for Experimental Cancer Research, Epalinges. (epfl.ch)
  • Accumulation of p300 mediates transcriptional repression of simian virus 40 enhancer in undifferentiated F9 embryonal carcinoma cells -- Ota et al. (aacrjournals.org)
  • Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. (elsevier.com)
  • These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription. (elsevier.com)
  • Transport of α-aminoisobutyric acid and 2-deoxy- d -glucose in African green monkey kidney cells was measured 8 to 100 hr following permissive simian virus 40 infection. (aacrjournals.org)
  • The absence of transport enhancement in permissive simian virus 40 infection suggests that the augmented transport of viral-transformed cell lines devolves upon altered host genome function. (aacrjournals.org)
  • Permissive and nonpermissive simian virus 40 (SV40)-infected cells were ultrastructurally analyzed. (asm.org)
  • CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. (nih.gov)
  • Simian virus 40 large T antigen (SV40LTAg) primer specific DNA amplification in human pleural mesothelioma tissue. (bmj.com)
  • Hmeljak J, Kern I, Cör A. No implication of Simian virus 40 in pathogenesis of malignant pleural mesothelioma in Slovenia. (cdc.gov)
  • Simian virus 40 in humans. (greenmedinfo.com)
  • But, tainted vaccines were knowingly distributed throughout the country because the doctors believed at the time that animal viruses posed no health threat to humans, that the virus that harms monkeys could not harm humans. (larrysmusings.com)
  • So, humans are not immune to all viruses and all bacteria that also attack and harm animals. (larrysmusings.com)
  • EEE is a mosquito-borne virus that can cause serious, and sometimes deadly, disease in humans and equines. (scienceblogs.com)
  • Two upcoming studies by scientists at the National Cancer Institute (NCI), provide further evidence that exposure to simian virus 40 (SV40) is not associated with cancer in humans. (news-medical.net)
  • Researchers have hypothesized that SIVs led to the transmission of HIV (human immunodeficiency virus) in humans. (historyofvaccines.org)
  • Because of research done with SV40 in animal models, there has been some concern that the virus could cause cancer in humans. (cdc.gov)
  • This invention can be used to prevent humans from alphavirus-induced encephalitides in the event of a bioterrorism attack or biowarfare in which alphaviruses such as Venezuelan (VEEV), eastern (EEEV) and western (WEEV) equine encephalitis viruses are deliberately released to humans, a natural outbreak of alphaviruses, and an accidental exposure to alphaviruses in laboratory. (freepatentsonline.com)
  • Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication. (uniprot.org)
  • Primer-DNA formation during simian virus 40 DNA replication in vitro. (asm.org)
  • Studies of simian virus 40 (SV40) DNA replication in vitro have identified a small (approximately 30-nucleotide) RNA-DNA hybrid species termed primer-DNA. (asm.org)
  • Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro. (semanticscholar.org)
  • Purification of replication protein C, a cellular protein involved in the initial stages of simian virus 40 DNA replication in vitro. (duke.edu)
  • Journal of Virology , 40 (3), 729-734. (elsevier.com)
  • Roman, A & Edenberg, H 1981, ' Ultraviolet irradiation inhibits encapsidation of simian virus 40 chromatin ', Journal of Virology , vol. 40, no. 3, pp. 729-734. (elsevier.com)
  • Meek, DW & Eckhart, W 1990, ' Mutation of the serine 312 phosphorylation site does not alter the ability of mouse p53 to inhibit simian virus 40 DNA replication in vivo ', Journal of Virology , vol. 64, no. 4, pp. 1734-1744. (dundee.ac.uk)
  • Therefore, we analyzed the efficacy of a recombinant simian virus 40 vector (rSV40) encoding IGF-I (rSVIGF-I) to prevent cirrhosis progression. (nih.gov)
  • These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses. (nih.gov)
  • This resulted in a 30-40% reduction in serum cholesterol, with the recombinant LDL receptor being detectable for up to six months. (bmj.com)
  • Fishman, Glenn I. / Cardiac and skeletal myopathy in β myosin heavy-chain simian virus 40 tsA58 transgenic mice . (elsevier.com)
  • 1998 ) A multi-institutional study confirms the presence and expression of Simian virus 40 in human malignant mesotheliomas. (bmj.com)
  • No association between simian virus 40 and diffuse malignant mesothelioma of the pleura in Iranian patients: a molecular and epidemiologic case-control study of 60 patients. (cdc.gov)
  • No detection of simian virus 40 in malignant mesothelioma in Korea. (cdc.gov)
  • It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. (bvsalud.org)
  • But here it is, 2011, and there are still papers that have to put in their abstracts "Simian virus-40 (SV40) is a DNA tumour virus that was introduced into the human population with contaminated poliovirus vaccine, and its role in mesothelioma is widely debated " and "Malignant mesothelioma is predominantly caused by asbestos exposure, although the association of Simian virus 40 in its pathogenesis is currently still under debate . (scienceblogs.com)
  • The large tumor antigen (LTag) of simian virus 40, an AAA(+) protein, is a hexameric helicase essential for viral DNA replication in eukaryotic cells. (rcsb.org)
  • During normal maturation the majority of pulse-labeled simian virus 40 DNA progresses from chromatin to previrions and virions within 5 h. (elsevier.com)
  • In heavily irradiated cultures (108 J m -2 ) most of the simian virus 40 DNA synthesized immediately before irradiation remains as chromatin for at least 5 h. (elsevier.com)
  • From 1955 to 1963, an estimated 10-30% of polio vaccines administered in the US were contaminated with simian virus 40 (SV40). (cdc.gov)
  • The virus came from monkey kidney cell cultures used to make polio vaccines at that time. (cdc.gov)
  • How these viruses initiate the lytic event is largely unknown. (umass.edu)
  • Simian virus 40 (SV40) mRNA was isolated by hybridization of cytoplasmic RNA, from SV40-infected BS-C-1 monkey cells early in lytic infection, to SV40 DNA immobilized on Sepharose. (pubfacts.com)
  • Simian Virus 40 (SV40) and Mouse Polyoma Virus (PY) are small DNA tumor viruses that have been used extensively to study cellular transformation. (nih.gov)
  • The "tumor antigen" (T antigen), a nonstructural protein expressed by the viruses, is responsible for cell transformation in animal models. (aacrjournals.org)
  • Enumeration of the simian virus 40 early region elements necessary for human cell transformation. (semanticscholar.org)
  • Mulatero et al 1 report failure to detect Simian virus 40 (SV40) DNA in 12 British mesotheliomas. (bmj.com)
  • Closed-circular, superhelical DNA from simian virus 40 (SV40 DNA I) was treated with an excess of DNA-relaxing enzyme in the presence of increasing amounts of ethidium bromide (EtdBr). (pnas.org)
  • The efficiency of simian virus 40 (SV40) DNA replication is dependent on the structural organization of the regulatory region. (ntnu.edu.tw)
  • Cell-free translation of SV40-specific mRNA, prepared from these cells by hybridization of total cytoplasmic RNA to SV40 DNA, showed that the mRNA's specifying these two polypeptides were at least partially encoded by the SV40 portion of the hybrid viruses. (utah.edu)
  • Simian virus 40 has a high prevalence in hematological malignancies and probably plays a role in tumor pathogenesis. (greenmedinfo.com)
  • Mesothelioma, a malignancy associated with asbestos, has been recently linked to simian virus 40 (SV40). (pnas.org)
  • The role of simian virus 40 (SV40) in mesothelioma development, on the other hand, remains controversial. (biomedcentral.com)
  • Some mesothelioma cases show a possible connection between mesothelioma and simian virus 40 (SV40). (mesothelioma.com)
  • Studies have shown a strong correlation between those that have the virus and a development of mesothelioma, though further investigation is needed. (mesothelioma.com)
  • The relationship between simian virus 40 and mesothelioma. (kintera.org)
  • Type-C RNA virus gene expression in human tissue. (biomedsearch.com)
  • The molecular mechanisms by which the virus reproduces and alters cell function were previously unknown, and research into SV40 vastly increased biologists' understanding of gene expression and the regulation of cell growth. (wikipedia.org)
  • Nucleotide sequence of the simian virus 40 HindII + III restriction fragment A (second part of the T antigen gene). (cngb.org)
  • We developed an early-onset SCA6 mouse model using an adeno-associated virus (AAV)-based gene delivery system to ectopically express CACNA1A IRES-driven α1ACT SCA6 to test the potential of CACNA1A IRES-targeting therapies. (sciencemag.org)
  • Childhood herpes simplex virus-1 (HSV-1) encephalitis (HSE) may result from single-gene inborn errors of TLR3 immunity. (rupress.org)
  • 1994. T-antigen kinase inhibits simian virus 40 DNA replication by phospho-rylation of intact T antigen on serines 120 and 123. (springer.com)
  • However, in monkeys that are immunodeficient-due to, for example, infection with Simian immunodeficiency virus-SV40 acts much like the human JC and BK polyomaviruses, producing kidney disease and sometimes a demyelinating disease similar to PML. (wikipedia.org)
  • Simian immunodeficiency viruses are retroviruses found in primates. (historyofvaccines.org)
  • Two new defective adenovirus 2/simian virus 40 hybrids (Ad2+ D1 and Ad2 + D2) have been isolated from the population known as Ad2++ HEY (Lewis & Rowe, 1970). (cshl.edu)
  • Simian virus 40 (SV40) was an accidental contaminant of vaccines produced in monkey kidney tissue cultures in the 1950s and early 1960s, including a parenteral adenovirus vaccine given to several hundred thousand US military recruits. (oup.com)
  • Epitopes described in Dissection of H-2Db-restricted cytotoxic T-lymphocyte epitopes on simian virus 40 T antigen by the use of synthetic peptides and H-2Dbm mutants. (iedb.org)
  • This set of primers amplifies the regulatory region and the origin of replication of the virus. (levelofhealth.com)
  • It does not act synergistically in determining virus inactivation with UV light and is equally effective on wild type and a late mutant of SV40. (biomedsearch.com)
  • CV1 monkey cell infection experiments indicated that mutant viruses that lack small t replicate less efficiently than the wild-type virus. (meta.org)
  • SV40 was introduced unintentionally into millions of people via contaminated poliomyelitis virus vaccines between 1955 and 1963. (biomedcentral.com)
  • Even me, not a doctor, was aware since early 2000 that polio vaccinations between 1955 and 1963 were tainted with this simian virus (from monkeys). (larrysmusings.com)
  • O descubrimento do SV40 en monos revelou que entre 1955 e 1963 moitas persoas foron inoculadas inadvertidamente co virus SV40, xa que este contaminaba as vacinas da polio , que se facían utilizando células de monos (por exemplo, nos Estados Unidos o 90% dos nenos e o 60% dos adultos foron inoculados con estas vacinas ), aínda que non se observou ningún efecto nocivo. (wikipedia.org)
  • Phosphorylation of simian virus 40 (SV40) T antigen on threonine 124 activates viral DNA replication in vivo and in vitro. (cshl.edu)
  • Co-operation of simian virus 40 T antigen and insulin receptor substrate-1 in protection from apoptosis induced by interleukin-3 withdrawal. (nih.gov)