A group of enzymes with the general formula CMP-N-acetylneuraminate:acceptor N-acetylneuraminyl transferase. They catalyze the transfer of N-acetylneuraminic acid from CMP-N-acetylneuraminic acid to an acceptor, which is usually the terminal sugar residue of an oligosaccharide, a glycoprotein, or a glycolipid. EC 2.4.99.-.
A nucleoside monophosphate sugar which donates N-acetylneuraminic acid to the terminal sugar of a ganglioside or glycoprotein.
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that are common in the marine environment and on the surfaces and in the intestinal contents of marine animals. Some species are bioluminescent and are found as symbionts in specialized luminous organs of fish.
Cytidine (dihydrogen phosphate). A cytosine nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.
An enzyme that forms CMP-acylneuraminic acids, which donate the N-acylneuraminic acid residues to the terminal sugar residue of a ganglioside or glycoprotein. EC 2.7.7.43.
A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
Enzymes catalyzing the transfer of fucose from a nucleoside diphosphate fucose to an acceptor molecule which is frequently another carbohydrate, a glycoprotein, or a glycolipid molecule. Elevated activity of some fucosyltransferases in human serum may serve as an indicator of malignancy. The class includes EC 2.4.1.65; EC 2.4.1.68; EC 2.4.1.69; EC 2.4.1.89.
SUGARS containing an amino group. GLYCOSYLATION of other compounds with these amino sugars results in AMINOGLYCOSIDES.
Transmembrane proteins consisting of a lectin-like domain, an epidermal growth factor-like domain, and a variable number of domains that are homologous to complement regulatory proteins. They are important cell adhesion molecules which help LEUKOCYTES attach to VASCULAR ENDOTHELIUM.
An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)
The study of the origin, structure, development, growth, function, genetics, and reproduction of organisms which inhabit the OCEANS AND SEAS.
The characteristic 3-dimensional shape of a carbohydrate.
Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Transferases are enzymes transferring a group, for example, the methyl group or a glycosyl group, from one compound (generally regarded as donor) to another compound (generally regarded as acceptor). The classification is based on the scheme "donor:acceptor group transferase". (Enzyme Nomenclature, 1992) EC 2.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Proteins prepared by recombinant DNA technology.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
The rate dynamics in chemical or physical systems.
Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of galactose with an alcohol to form an acetal. They include both alpha- and beta-galactosides.
A compound formed when iodoacetic acid reacts with sulfhydryl groups in proteins. It has been used as an anti-infective nasal spray with mucolytic and expectorant action.
Galactosides in which the oxygen atom linking the sugar and aglycone is replaced by a sulfur atom.
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Rare leukoencephalopathy with infantile-onset accumulation of Rosenthal fibers in the subpial, periventricular, and subependymal zones of the brain. Rosenthal fibers are GLIAL FIBRILLARY ACIDIC PROTEIN aggregates found in ASTROCYTES. Juvenile- and adult-onset types show progressive atrophy of the lower brainstem instead. De novo mutations in the GFAP gene are associated with the disease with propensity for paternal inheritance.
A family of the order Rodentia which contains 49 genera. Some of the more common genera are MARMOTA, which includes the marmot and woodchuck; Sciurus, the gray squirrel, S. carolinensis, and the fox squirrel, S. niger; Tamias, the eastern and western chipmunk; and Tamiasciurus, the red squirrel. The flying squirrels, except the scaly-tailed Anomaluridae, also belong to this family.
CXCR receptors with specificity for CXCL12 CHEMOKINE. The receptors may play a role in HEMATOPOIESIS regulation and can also function as coreceptors for the HUMAN IMMUNODEFICIENCY VIRUS.
Time period from 1501 through 1600 of the common era.
The religion stemming from the life, teachings, and death of Jesus Christ: the religion that believes in God as the Father Almighty who works redemptively through the Holy Spirit for men's salvation and that affirms Jesus Christ as Lord and Savior who proclaimed to man the gospel of salvation. (From Webster, 3d ed)
Traditional Arabic methods used in medicine in the ARAB WORLD.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
The portion of an interactive computer program that issues messages to and receives commands from a user.
Sequential operating programs and data which instruct the functioning of a digital computer.

Sialyltransferase isoforms are phosphorylated in the cis-medial Golgi on serine and threonine residues in their luminal sequences. (1/910)

ST6Gal-I (alpha2,6-sialyltransferase) is expressed as two isoforms, STTyr and STCys, which exhibit differences in catalytic activity, trafficking through the secretory pathway, and proteolytic processing and secretion. We have found that the ST6Gal-I isoforms are phosphorylated on luminal Ser and Thr residues. Immunoprecipitation of 35S- and 32P-labeled proteins expressed in COS-1 cells suggests that the STTyr isoform is phosphorylated to a greater extent than the STCys isoform. Analysis of domain deletion mutants revealed that STTyr is phosphorylated on stem and catalytic domain amino acids, whereas STCys is phosphorylated on catalytic domain amino acids. An endoplasmic reticulum retained/retrieved chimeric Iip33-ST protein demonstrates drastically lower phosphorylation than does the wild type STTyr isoform. This suggests that the bulk of the ST6Gal-I phosphorylation is occurring in the Golgi. Treatment of cells with the ionophore monensin does not significantly block phosphorylation of the STTyr isoform, suggesting that phosphorylation is occurring in the cis-medial Golgi prior to the monensin block. This study demonstrates the presence of kinase activities in the cis-medial Golgi and the substantial phosphorylation of the luminal sequences of a glycosyltransferase.  (+info)

Expression cloning of mouse cDNA of CMP-NeuAc:Lactosylceramide alpha2,3-sialyltransferase, an enzyme that initiates the synthesis of gangliosides. (2/910)

Expression cloning of a cDNA for the alpha2,3-sialyltransferase (GM3 synthase) (EC 2.4.99.-) gene was performed using a GM3-lacking mouse fibroblast line L cell and anti-GM3 monoclonal antibody. Plasmids from a cDNA library generated with poly(A)+ RNA of a mouse fibrosarcoma line CMS5j and pdl3027 (polyoma T antigen) were co-transfected into L cells. The isolated cDNA clone pM3T-7 predicted a type II membrane protein with 13 amino acids of cytoplasmic domain, 17 amino acids of transmembrane region, and a large catalytic domain with 329 amino acids. Introduction of the cDNA clone into L cells resulted in the neo-synthesis of GM3 and high activity of alpha2,3-sialyltransferase. Among glycosphingolipids, only lactosylceramide showed significant activity as an acceptor, indicating that this gene product is a sialyltransferase specific for the synthesis of GM3. An amino acid sequence deduced from the cloned cDNA showed the typical sialyl motif with common features among alpha2,3-sialyltransferases. Among various mouse tissues, brain, liver, and testis showed relatively high expression of a 2.3-kilobase mRNA, whereas all tissues, more or less, expressed this gene.  (+info)

Regulation of capsular polysialic acid biosynthesis by temperature in Pasteurella haemolytica A2. (3/910)

The capsular polysaccharide of Pasteurella haemolytica A2 consists of a linear polymer of N-acetylneuraminic acid (Neu5Ac) with alpha(2-8) linkages. The production of this polymer is strictly regulated by the growth temperature and above 40 degrees C no production is detected. Analysis of the enzymatic activities directly involved in its biosynthesis reveals that Neu5Ac lyase, CMP-Neu5Ac synthetase and polysialyltransferase are involved in this regulation. Very low activities were found in P. haemolytica grown at 43 degrees C (at least 25 times lower than those observed when the growth temperature was 37 degrees C). The synthesis of these enzymes increased rapidly when bacteria grown at 43 degrees C were transferred to 37 degrees C and decreased dramatically when cells grown at 37 degrees C were transferred to 43 degrees C. These findings indicate that the cellular growth temperature regulates the synthesis of these enzymes and hence the concentration of the intermediates necessary for capsular polysaccharide genesis in P. haemolytica A2.  (+info)

Molecular cloning of a novel alpha2,3-sialyltransferase (ST3Gal VI) that sialylates type II lactosamine structures on glycoproteins and glycolipids. (4/910)

A novel member of the human CMP-NeuAc:beta-galactoside alpha2, 3-sialyltransferase (ST) subfamily, designated ST3Gal VI, was identified based on BLAST analysis of expressed sequence tags, and a cDNA clone was isolated from a human melanoma line library. The sequence of ST3Gal VI encoded a type II membrane protein with 2 amino acids of cytoplasmic domain, 32 amino acids of transmembrane region, and a large catalytic domain with 297 amino acids; and showed homology to previously cloned ST3Gal III, ST3Gal IV, and ST3Gal V at 34, 38, and 33%, respectively. Extracts from L cells transfected with ST3Gal VI cDNA in a expression vector and a fusion protein with protein A showed an enzyme activity of alpha2, 3-sialyltransferase toward Galbeta1,4GlcNAc structure on glycoproteins and glycolipids. In contrast to ST3Gal III and ST3Gal IV, this enzyme exhibited restricted substrate specificity, i.e. it utilized Galbeta1,4GlcNAc on glycoproteins, and neolactotetraosylceramide and neolactohexaosylceramide, but not lactotetraosylceramide, lactosylceramide, or asialo-GM1. Consequently, these data indicated that this enzyme is involved in the synthesis of sialyl-paragloboside, a precursor of sialyl-Lewis X determinant.  (+info)

Molecular cloning and functional expression of two members of mouse NeuAcalpha2,3Galbeta1,3GalNAc GalNAcalpha2,6-sialyltransferase family, ST6GalNAc III and IV. (5/910)

Two cDNA clones encoding NeuAcalpha2,3Galbeta1,3GalNAc GalNAcalpha2, 6-sialyltransferase have been isolated from mouse brain cDNA libraries. One of the cDNA clones is a homologue of previously reported rat ST6GalNAc III according to the amino acid sequence identity (94.4%) and the substrate specificity of the expressed recombinant enzyme, while the other cDNA clone includes an open reading frame coding for 302 amino acids. The deduced amino acid sequence is not identical to those of other cloned mouse sialyltransferases, although it shows the highest sequence similarity with mouse ST6GalNAc III (43.0%). The expressed soluble recombinant enzyme exhibited activity toward NeuAcalpha2, 3Galbeta1, 3GalNAc, fetuin, and GM1b, while no significant activity was detected toward Galbeta1,3GalNAc or asialofetuin, or the other glycoprotein substrates tested. The sialidase sensitivity of the 14C-sialylated residue of fetuin, which was sialylated by this enzyme with CMP-[14C]NeuAc, was the same as that of ST6GalNAc III. These results indicate that the expressed enzyme is a new type of GalNAcalpha2,6-sialyltransferase, which requires sialic acid residues linked to Galbeta1,3GalNAc residues for its activity; therefore, we designated it mouse ST6GalNAc IV. Although the substrate specificity of this enzyme is similar to that of ST6GalNAc III, ST6GalNAc IV prefers O-glycans to glycolipids. Glycolipids, however, are better substrates for ST6GalNAc III.  (+info)

Up-regulation of the alpha2,6-sialyltransferase messenger ribonucleic acid increases glycoconjugates containing alpha2, 6-linked sialic acid residues in granulosa cells during follicular atresia of porcine ovaries. (6/910)

The sugar chains in cellular glycoconjugates have many biological functions. Extensive morphological development and remodeling occur in the ovary of female animals. This caused us to study glycobiological characteristics of ovarian cells, particularly granulosa cells that undergo apoptosis during follicular atresia. The lectin Sambucus sieboldiana agglutinin (SSA) specific for Siaalpha2,6Gal/GalNAc showed positive staining for granulosa cells only in atretic follicles of porcine ovaries by lectin histochemistry. Lectin blot analysis for SSA demonstrated specific glycoproteins only in atretic follicles. Furthermore, we performed analysis of backbone structures of SSA-positive glycans carried by granulosa cell glycoproteins increased during atresia by glycosidase treatment. Most of these structures were Siaalpha2,6Galbeta1,4GlcNAc on complex-type N-glycans, suggesting that only ST6Gal I of four distinct alpha2,6-sialyltransferases catalyzes alpha2,6-sialic acid transfer in most of the increased glycoproteins of granulosa cells during follicular atresia. Reverse transcription-polymerase chain reaction analysis demonstrated that the expression of ST6Gal I mRNA was up-regulated in granulosa cells during atresia. These results suggested that the alteration of glycoconjugates by ST6Gal I in granulosa cells during atresia is involved in some processes of ovarian follicular atresia and granulosa cell apoptosis.  (+info)

Identification of an alpha2,6-sialyltransferase induced early after lymphocyte activation. (7/910)

We have used mRNA differential display PCR to search for genes induced in activated T cells and we identified a gene encoding an alpha2,6-sialyltransferase (ST6GalNAc IV) that is rapidly induced in lymphocytes after antigen or mitogen stimulation. The 3.6 kb full-length cDNA clone (MK45) obtained contained a single open reading frame encoding a 302 amino acid protein and a 2.5 kb 3' untranslated region. MK45 expression in in vivo-activated CD8 T cells reached the highest level 4 h after antigen triggering and then declined rapidly to nearly base levels within 45 h. Northern blot analysis further revealed that MK45 expression was also induced in LPS-activated B cells and antigen-triggered CD4 T cells in vitro. MK45 expression was low or undetectable in most other mouse tissues examined, when compared to activated lymphocytes. Importantly, the mRNA expression level of other sialyltransferases remained largely unchanged during the early stage of lymphocyte activation. Finally, increased ecto-sialyltransferase activity and an altered sialylation pattern were demonstrated on the cell surface of early activated CD8 T cells. Our report identifies a candidate sialyltransferase gene that is involved in the early alteration of the sialylation pattern of cell surface molecules in activated lymphocytes.  (+info)

Multi-enzyme kinetic analysis of glycolipid biosynthesis. (8/910)

Gangliosides are acidic glycosphingolipids synthesized sequentially by a series of glycosyltransferases acting in parallel biosynthetic pathways. While most glycosyltransferases are highly specific, some, however, may catalyze equivalent steps in each pathway using different gangliosides as substrates (e.g. N-acetylgalactosaminyltransferase, sialyltransferase-IV). A multi-enzyme kinetic analysis was developed on the condition that serial enzymatic reactions operate below substrate saturation. A multi-enzyme kinetic analysis enabled a simultaneous calculation of the Vmax/Km value of each enzyme derived from the equilibrium concentration of the respective substrate. Substrate concentrations [S] were determined by radioactive labelling of gangliosides in intact cells with the precursor sugars [14C]galactose and [14C]glucosamine, followed by high-performance thin-layer chromatography and autoradiography of the radiolabelled glycolipids. On the basis of Michaelis-Menten kinetics, Vmax/Km values were derived from [S] by a system of linear equations. The procedure was used to analyze the development of the glycolipid composition during differentiation of rat gliomaxmurine neuroblastoma (NG108-15) cells. The Vmax/Km values calculated by multi-enzyme kinetic analysis were consistent with the kinetic data obtained with solubilized enzymes. Application of multi-enzyme kinetic analysis to published data on the correlation of enzyme activities with ganglioside levels in various cell lines and tissues indicated the validity of this method for analysis of the glycolipid biosynthesis, in particular, of its initial steps. On the basis of the kinetic analysis, it is suggested that the cell lines can be divided into two groups with respect to the substrate pools of GM3 used by sialyltransferase-II and N-acetylgalactosaminyltransferase-I. The first group encompasses the majority of the neuroblastoma cell lines and the embryonic rat brain where the two enzymes share a common pool of GM3. In the second group, the two enzymes do not compete for the same pool of GM3, indicating a different subcellular localization of CMP-NeuAc:GM3 alpha2-8-sialyltransferase and UDP-N-acetylgalactosaminyl:GM3 N-acetylgalactosaminyltransferase. In this study, the theory of a multi-enzyme kinetic analysis is discussed and its application to analysis of the glycolipid biosynthesis in neuroblastoma cells is demonstrated. A multi-enzyme kinetic analysis can be applied to other biosynthetic pathways and provides the advantage of analyzing kinetic data with intact cells or tissue samples.  (+info)

David Frommhold, Andreas Ludwig, M. Gabriele Bixel, Alexander Zarbock, Inna Babushkina, Melitta Weissinger, Sandra Cauwenberghs, Lesley G. Ellies, Jamey D. Marth, Annette G. Beck-Sickinger, Michael Sixt, Bärbel Lange-Sperandio, Alma Zernecke, Ernst Brandt, Christian Weber, Dietmar Vestweber, Klaus Ley, Markus Sperandio ...
Incubation of HeLa cells in the presence of millimolar concentrations of propionate, butyrate, or pentanoate increases the specific activity of CMP-sialic acid:lactosylceramide sialyltransferase 7-20-fold within 24 h. Longer-chain saturated fatty acids or acetate are much less effective, decanoate showing no induction. Unsaturated fatty acid analogs of butyrate and other compounds are ineffective. Only the three most effective compounds also produce characteristic smooth extended cell processes in HeLa cells. Butyrate (5 mM) induces the sialyltransferase after a 4-h lag, producing maximum specific activity by 24 h. The amount of sialyl-lactosylceramide, the glycolipid product of the enzyme, increases during that time 3.5 times more than in control cultures. No other glycosphingolipid enzyme is significantly altered by butyrate exposure. The cellular shape changes occur 2-3 h later than the increase of sialyltransferase activity, and both processes require the continuous presence of inducer and ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
article{a9d82ec1-f6e8-48c0-9e86-ffa33a7ea3ea, abstract = {,p,Aberrant cell-surface glycosylation patterns are present on virtually all tumors and have been linked to tumor progression, metastasis, and invasivity. We have shown that expressing a normally quiescent, glycoprotein-specific alpha2,6-sialyltransferase (ST6Gal1) gene in gliomas inhibited invasivity in vitro and tumor formation in vivo. To identify other glycogene targets with therapeutic potential, we created a focused 45-mer oligonucleotide microarray platform representing all of the cloned human glycotranscriptome and examined the glycogene expression profiles of 10 normal human brain specimens, 10 malignant gliomas, and 7 human glioma cell lines. Among the many significant changes in glycogene expression observed, of particular interest was the observation that an additional alpha2,6-sialyltransferase, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha2,6-sialyltransferase 5 (ST6GalNAcV), was ...
Reaktivität: Rind (Kuh), Hund, Ziege and more. 36 verschiedene ST3GAL5 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
The KOMP Repository Collection is located at the MMRRC at the University of California, Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
Complete information for ST3GAL5 gene (Protein Coding), ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Previously, we found that β-galactoside α2,6-sialyltransferase (ST6Gal I), an enzyme that adds sialic acids to N-linked oligosaccharides of glycoproteins and is frequently overexpressed in cancer cells, is up-regulated by ionizing radiation (IR) and cleaved to a form possessing catalytic activity comparable to that of the Golgi-localized enzyme. Moreover, this soluble form is secreted into the culture media. Induction of ST6Gal I significantly increased the migration of colon cancer cells via sialylation of integrin β1. Here, we further investigated the mechanisms underlying ST6Gal I cleavage, solubilization and release from cells, and addressed its functions, focusing primarily on cancer cell migration. We performed immunoblotting and lectin affinity assay to analyze the expression of ST6 Gal I and level of sialylated integrin β1. After ionizing radiation, migration of cells was measured by in vitro migration assay. α2, 6 sialylation level of cell surface was analyzed by flow cytometry. Cell
Accepted name: α-N-acetylneuraminate α-2,8-sialyltransferase Reaction: CMP-N-acetylneuraminate + α-N-acetylneuraminyl-(2→3)-β-D-galactosyl-R = CMP + α-N-acetylneuraminyl-(2→8)-α-N-acetylneuraminyl-(2→3)-β-D-galactosyl-R. For diagram of reaction click here.. Other name(s): cytidine monophosphoacetylneuraminate-ganglioside GM3; α-2,8-sialyltransferase; ganglioside GD3 synthase; ganglioside GD3 synthetase sialyltransferase; CMP-NeuAc:LM1(α2-8) sialyltranferase; GD3 synthase; SAT-2 Systematic name: CMP-N-acetylneuraminate:α-N-acetylneuraminyl-2,3-β-D-galactoside α-2,8-N-acetylneuraminyltransferase Comments: Gangliosides act as acceptors. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 67339-00-8. References: 1. Eppler, M.C., Morré, J.D. and Keenan, T.W. Ganglioside biosynthesis in rat liver: alteration of sialyltransferase activities by nucleotides. Biochim. Biophys. Acta 619 (1980) 332-343. [PMID: 7407217] 2. Higashi, H., Basu, M. and Basu, S. ...
CMP-sialic acid solution synthetase (CSAS) is a key enzyme of the sialylation pathway. glycosylation INTRODUCTION Cytidine monophosphate sialic acid synthetases (CSAS) are evolutionarily conserved enzymes mediating a key step in the biosynthetic pathway of sialylation. They generate the activated sugar donor CMP-sialic acid (CMP-Sia) from free sialic acid (Sia) and cytidine triphosphate (CTP). The product of CSAS CMP-Sia is usually utilized by sialyltransferase enzymes to modify the termini of glycans attached to glycoproteins and glycolipids and produce various sialoglycoconjugates [1]. These sialylated molecules are eventually delivered to the cell surface or secreted to extracellular milieu where they mediate a plethora of crucial biological functions [2 3 While organisms can possess multiple sialyltransferases (e.g. humans have twenty different sialyltransferase enzymes with distinct linkage and substrate specificities) there is usually only one CSAS (aka CMAS) enzyme mediating the sialic ...
BACKGROUND: Treatment with Bacillus Calmette-Guérin (BCG) is the gold standard adjuvant immunotherapy of non-muscle invasive bladder cancer (NMIBC), although it fails in one third of the patients. NMIBC expresses two tumor-associated O-linked carbohydrates: the disaccharide (Galβ1,3GalNAc) Thomsen-Friedenreich (T) antigen, and its sialylated counterpart (Siaα2,3Galβ1,3GalNAc) sialyl-T (sT), synthesized by sialyltransferase ST3GAL1, whose roles in BCG response are unknown. METHODS: The human bladder cancer (BC) cell line HT1376 strongly expressing the T antigen, was retrovirally transduced with the ST3GAL1 cDNA or with an empty vector, yielding the cell lines HT1376sT and HT1376T , that express, respectively, either the sT or the T antigens ...
TY - JOUR. T1 - Differentiation elicits negative regulation of human β-galactoside α2,6-sialyltransferase at the mRNA level in the HL-60 cell line. AU - Taniguchi, Akiyoshi. AU - Higai, Koji. AU - Hasegawa, Yuko. AU - Utsumi, Kunio. AU - Matsumoto, Kojiro. PY - 1998/12/18. Y1 - 1998/12/18. N2 - We studied the regulation of the β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During HL-60 cell line differentiation, cell surface levels of α2,6-sialic acids expression decreased, as measured by flow cytometric analysis using Sambucus nigra agglutinin (SNA). Activities of hST6Gal I and levels of hST6Gal I mRNA dramatically decreased after 1 day of stimulation. Using reverse transcription polymerase chain reaction (PT-PCR), we found the major hST6Gal I mRNA isoform in HL-60 cells contains 5-untranslated exons Y and Z. These results suggest that the ...
Accepted name: α-N-acetylgalactosaminide α-2,6-sialyltransferase Reaction: CMP-N-acetylneuraminate + glycano-(1→3)-(N-acetyl-α-D-galactosaminyl)-glycoprotein = CMP + glycano-[(2→6)-α-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein. Systematic name: CMP-N-acetylneuraminate:glycano-1,3-(N-acetyl-α-D-galactosaminyl)-glycoprotein α-2,6-N-acetylneuraminyltransferase Comments: α-N-Acetylgalactosamine linked to threonine or serine is also an acceptor, when substituted at the 3-position. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 71124-50-0. References: 1. Sadler, J.E., Rearick, J.I. and Hill, R.L. Purification to homogeneity and enzymatic characterization of an α-N-acetylgalactosaminide α2→6 sialyltransferase from porcine submaxillary glands. J. Biol. Chem. 254 (1979) 5934-5941. [PMID: 447688] ...
sialyltransferase (EC 2.4.99.-); β-galactoside α-2,6-sialyltransferase (EC 2.4.99.1); α-N-acetylgalactosaminide α-2,6-sialyltransferase (EC 2.4.99.3); β-galactoside α-2,3-sialyltransferase (EC 2.4.99.4); N-acetyllactosaminide α-2,3-sialyltransferase (EC 2.4.99.6); (α-N-acetyl-neuraminyl-2,3-β-galactosyl-1,3)-N-acetylgalactosaminide α-2,6-sialyltransferase (EC 2.4.99.7); α-N-acetyl-neuraminide α-2,8-sialyltransferase (EC 2.4.99.8); lactosylceramide α-2,3-sialyltransferase (EC 2.4.99.9 ...
ST3GAL5 antibody (ST3 beta-galactoside alpha-2,3-sialyltransferase 5) for WB. Anti-ST3GAL5 pAb (GTX103710) is tested in Human samples. 100% Ab-Assurance.
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ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Proteins available through Novus Biologicals. Browse our ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Protein catalog backed by our Guarantee+.
St6galnac6 (untagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6), transcript variant 2, (10ug), 10 µg.
Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.
Expression Plasmids. A sequence-verified human TSHR cDNA cloned into pSVL (courtesy of Dr. G. Vassart, Brussels, Belgium) was used. Respectively, for lectin binding ELISA, we used a TSHR-GFP fusion protein (courtesy of Dr R. Latif, New York) in which the human TSHR sequence lacking the stop codon was ligated into the mammalian expression vector pEGFP-N1 (BD Biosciences Clontech, Palo Alto, CA). For SIAT8a, we used the pcDNA3.1 GeneStorm Expression-Ready Clone RG001497 (Invitrogen, Carlsbad, CA). The full-length cDNAs encoding the human SIAT1 and SIAT4a were subcloned into the eukaryotic expression vector pcDNA3.1/V5-His C (Invitrogen, Paisley, UK).. Tissue Samples. All AFTNs were identified by ultrasound and scintigraphy. All preoperatively identified nodules were also identified at surgery and postoperatively characterized by histology according to the World Health Organization criteria (Hedinger, 1988). Somatic TSH receptor mutations in the hot nodules were determined previously by denaturing ...
Su Bèlgiu est su segundu istadu in su mundu chi at aprovadu - reconnoschende·lu cun una lege - su cojuiu intre persones omosessuales. Sa lege est nàschida, comente fiat acontèssidu in Olanda - su primu paisu a las reconnòschere, in su 2000 - cando su podere fiat in manos de sos progressistas làicos. Fiat su 30 de ghennàrgiu de su 2003, duncas non sèculos a como, ma in tempus reghente. Pro unos cantos meses est abarradu in vigore su bedu pro sos omosessuales istràngios, posca cantzelladu cun una regulamentatzione noa, in vigore dae su 2004. Sas règulas noas previdint, sena leare in cunsideru su sessu de sos chi si cherent cojuare inie, chi nessi unu de sos duos interessados siat residente in Bèlgiu mìnimu tres meses. Sas règulas noas cunsentint fintzas sadotzione cungiunta de minores siat chi siant fìgios naturales de unu de sos duos isposos, siat chi siant fìgios de ànima, leados duncas dae unistitutu autorizadu. Unas cantas curiosidades: a su 12 de trìulas, in su 2015 sas ...
ST8SIA2 (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Overexpression Lysate (Denatured). Tested Reactivity: Hu. Validated: WB. Backed by our 100% Guarantee.
bifunctional CMP-N-acetylneuraminate : lacto-N-neotetraose α-2,3-sialyltrasferase / L1 LOS α-2,6-sialyltransferase (Lst;NGO_1081 ...
Vacsinou nosim saty alebo sukne s jednoduchymi bluzkami/trickami/koselami. Sem tam si dam nohavice, tych mam asi len trojo a nenosim casto. Rifle nosim len na nakup alebo ked ideme niekam von do prirody, su to pre mna teplaky na von. Vacsinu siat mam vzorovanu takze ostatne veci sa snazim mat jednofarebné. Vacsinou mam cierne topanky a aj cierne kabelky. Bezne nosim kabaty, bundy nosim len k teplakom a rifliam. Pri vybere nohavic, siat a sukien sa snazim aby som mala zvyrazneny pas a odputala pozornost od velkeho zadku ...
View mouse St6gal1 Chr16:23224740-23360350 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4. . ...
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sialyltransferase 7 ((alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase) D ...
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TIMITY. Th Francois Roche. New Territories MindMachineMakingMyth . A research on NEOTENY rOB fAB sTASE insTASE eXsTASE inTIMITY..... TIMITY! New Territories MindMachineMakingMyth . A research on NEOTENY rOB fAB sTASE insTASE eXsTASE inTIMITY..... Th Francois Roche. New Territories MindMachineMakingMyth . A research on NEOTENY rOB fAB sTASE insTASE eXsTASE inTIMITY..... TIMITY. Th Francois Roche. ...
Complete information for ST8SIA6 gene (Protein Coding), ST8 Alpha-N-Acetyl-Neuraminide Alpha-2,8-Sialyltransferase 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Gyvūnams, tame tarpe ir žmonėms, į vidų vartojant sidabro preparatus įvairiuose audiniuose kaupiasi sidabro junginiai.[4] Dalis sidabro junginių pasižymi jautrumu šviesai, tas šviesos veikiami dalis bespalvių ar silpnai spalvotų sidabro junginių skyla išskirdami gryno metalo daleles ar sidabro sulfidą. Paprastai šios medžiagos mikroskopinėmis dalelėmis nusėda odoje, kur atrodo kaip tamsaus pigmento dariniai. Tokia organizmo būklė ir vadinama argyrija.. Chroniškas sidabro junginių valgymas gali sukelti sidabro pigmentų susidarymą organuose (tame tarpe ir akyse), kurie būna daug laiko apšviesti.[5] Sidabro dalelių nusėdimas junginėje nėra kenksmingas organizmui, bet sidabras gali nusėsti akyje (pvz., lęšiuke) ir taip pažeisti regą.. Vietinė argyrija dažnai susidaro išoriškai vartojant sidabro preparatus, pvz., akių lašus.. Bendrąją argyriją sukelia ilgą laiką į organizmą patenkantys (nuryjami ar įkvepiami) sidabro junginiai. Taip gali nutikti ...
ST6GALNAC4 antibody [N2C3] (ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4) for WB. Anti-ST6GALNAC4 pAb (GTX119976) is tested in Human samples. 100% Ab-Assurance.
The status of each map region is indicated by a symbol, which describes the type of feature, and a colour, which indicates the completeness of that feature in a map region. ...
Rasa Martinkienė. VUL SK Vaikų ligoninė. Viršutinių kvėpavimo takų infekcijomis ikimokyklinio amžiaus vaikai gali sirgti daugiau nei 8-10 kartų per metus. Kosulys įprastai prasideda po 1-2 dienų nuo ligos pradžios ir vidutiniškai trunka apie 5 dienas. Dažniausia vaikų užtrukusio ūminio kosulio priežastis yra povirusinis ar poinfekcinis kosulys, kuris paprastai praeina negydomas. Lėtiniu vaikų kosuliu vadinamas toks kosulys, kuris užtrunka daugiau nei 4 savaites. Tik išsiaiškinus lėtinio kosulio priežastis, galimas sėkmingas jo gydymas. Lėtinis vaikų kosulys skirstomas į specifinį (žinomos priežastys) ir nespecifinį. Kosulio priežastys gali būti susijusios ne tik su viršutinių ir apatinių kvėpavimo takų ligomis, bet ir su širdies, virškinamojo trakto, nervų sistemos ar išorinės klausomosios landos ligomis. Todėl lėtinis kosulys - vienas iš nusiskundimų, kurio priežasčiai patikslinti reikalinga otorinolaringologo apžiūra. Kosulys vaikams yra ...
Nano Lett., 2017, 17 (10), pp 5931-5937. M. Pfender , N. Aslam , P. Simon , D. Antonov , G. Thiering , S. Burk , F. Fávaro de Oliveira , A. Denisenko , H. Fedder , J. Meijer , J. A. Garrido , A. Gali , T. Teraji , J. Isoya , M. W. Doherty , A. Alkauskas , A. Gallo , A. Grüneis , P. Neumann , J. Wrachtrup. Online Reference ...
Kauno technologijos universiteto (KTU) Architektūros ir statybos instituto Statybinės fizikos laboratorijos mokslininko dr. Edmundo Monstvilo teigimu, bet kuri statybos technologija, jei ji įgyvendinama laikantis standartų, savo energetinėmis charakteristikomis gali tenkinti A+ energinio naudingumo klasės reikalavimus.. „Kalbant apie statybą papildomai nenaudojant termoizoliacinių medžiagų, pasiekti A+ energinio naudingumo klasę būtų sudėtinga - čia reiktų itin storų sienų. Žinoma, jeigu statomas šiaudinis namas, konstrukcijoms naudojami stambūs šiaudų blokai ir jie nutinkuoti iš abiejų pusių, pasiekti aukštus energinio efektyvumo reikalavimus galima be problemų. Tokia statyba bus tikrai ekologiška, - sakė dr. E. Monstvilas.. Visgi mokslininkas abejojo, ar vien tik iš molio, nenaudojant papildomos termoizoliacijos, pavyktų savo rankomis nudrėbti A ar A+ energinio naudingumo klasės namą. Tokių žmonių, kuriems tai būtų pavykę, jam dar nėra tekę ...
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Fibroblast growth factor receptors (FGFRs) have been implicated in the malignant transformation and chemoresistance of epithelial ovarian cancer; however, the underlying molecular mechanisms are poorly understood. Increased sialyltransferase activity that enhances protein sialylation is an important post‑translational process promoting cancer progression and malignancy. In the present study, α2,6‑sialyltransferase (ST6Gal‑I) overexpression or knockdown cell lines were developed, and FGFR1 was examined to understand the effect of sialylation on migration and drug resistance, and the underlying mechanisms. It was identified that cells with ST6Gal‑I overexpression had increased cell viability and migratory ability upon serum deprivation. Moreover, ST6Gal‑I overexpression cells had strong resistance to paclitaxel, as demonstrated by low growth inhibition rate and cell apoptosis level. A mechanistic study showed that ST6Gal‑I overexpression induced high α2,6‑sialylation of FGFR1 and ...
Low-passage-number clinical influenza virus isolates often appear insensitive to NAI when tested in MDCK or other laboratory cell lines. This precludes the detection of changes in viral drug sensitivity (resistance) between pre- and posttreatment isolates and can give rise to false-positive identification of resistance in population screening. This study was undertaken to test whether a genetically engineered increase in the density of human influenza virus receptors on the surface of MDCK cells can make the virus more sensitive to NAI in these cells. To address this question, we permanently transfected MDCK cells with the gene of human 2,6-sialyltransferase.. Stable transfection with the SIAT1 gene increased the concentration of 6-linked sialic acids on the surface of MDCK cells about twofold. This effect is consistent with enhanced α-2,6-sialylation in several other mammalian cell lines after their transfection with SIAT1 (6, 10, 12, 24, 25). We found that a typical non-egg-adapted human H1N1 ...
Citation: Murdoch, B., Fu, A., Meng, Y., Li, C., Hansen, C., Snelling, W.M., Moore, S.S. 2004. Assignment of the SIAT4A gene to bovine chromosome 14 by linkage mapping of an associated microsatellite. Animal Genetics 35:146-147. Interpretive Summary: A new DNA marker for the Sialyltransferase 4A (SIAT4A) gene was developed and mapped. The marker was developed from the CHORI-240 bacterial artificial chromosome library. Cattle from an Angus-based commercial seedstock line, and two USDA-MARC reference families were genotyped. The USDA-MARC reference family genotypes were used to map the marker onto cattle chromosome 14. Technical Abstract: CHORI-240 bovine bacterial artificial chromosome library high density filters were probed with gene-specific overgo primers for Sialyltransferase 4A (SIAT4A). All positive clones were confirmed by polymerase chain reaction (PCR) with different gene-specific primers. Subsequently the clones were digested with Sau3 AI and subcloned into the E. coli cloning vector ...
ST3Gal V (or GM3 synthase) is the sialyltransferase that catalyses the initial step in the biosynthesis of most complex gangliosides from lactosylceramide. ST3Gal V plays a key regulatory role in determining the cell surface ganglioside profile and, consequently, in modulating a large variety of ganglioside-dependent cellular events, such as cell proliferation and differentiation, adhesion, apoptosis, oncogenesis. In addition, a homozygous loss-of-function mutation in the hST3Gal V gene is cause of an autosomal recessive infantile-onset symptomatic epilepsy syndrome. Recently, we have identified a hST3Gal V mRNA variant containing an additional translation start codon, located upstream and in-frame with that considered unique translation initiation site in the human GM3 synthase gene, providing the first evidence of the existence of two differentially N-terminal extended isoforms of the protein [1]. The functional relevance of the longer isoform has to be defined yet, and a study to define its ...
Activation of an aberrant glycosylation pathway in cancer cells can lead to expression of the onco-foetal sialyl-Tn (sTn) antigen. STn is a truncated O-glycan containing a sialic acid α-2,6 linked to GalNAc α-O-Ser/Thr and is associated with an adverse outcome and poor prognosis in cancer patients. The biosynthesis of the sTn antigen has been linked to the expression of the sialytransferase ST6GalNAc1, and also to mutations in and loss of heterozygosity of the COSMC gene. sTn neo- or over-expression occurs in many types of epithelial cancer including gastric, colon, breast, lung, oesophageal, prostate and endometrial cancer. sTn is believed to be carried by a variety of glycoproteins and may influence protein function and be involved in tumour development. This review discusses how the role of sTn in cancer development and tumour cell invasiveness might be organ specific and occur through different mechanisms depending on each cancer type or subtype. As the sTn-antigen is expressed early in
Expression of ST6GALNAC2 (SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM) in oral mucosa tissue. Antibody staining with HPA048849 in immunohistochemistry.
Expression of ST6GALNAC2 (SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM) in esophagus tissue. Antibody staining with HPA048849 in immunohistochemistry.
TY - JOUR. T1 - Neuraminidase-dependent degradation of polysialic acid is required for the lamination of newly generated neurons. AU - Sajo, Mari. AU - Sugiyama, Hiroki. AU - Yamamoto, Hideaki. AU - Tanii, Takashi. AU - Matsuki, Norio. AU - Ikegaya, Yuji. AU - Koyama, Ryuta. PY - 2016/1/5. Y1 - 2016/1/5. N2 - Hippocampal granule cells (GCs) are generated throughout the lifetime and are properly incorporated into the innermost region of the granule cell layer (GCL). Hypotheses for the well-regulated lamination of newly generated GCs suggest that polysialic acid (PSA) is present on the GC surface to modulate GC-to-GC interactions, regulating the process of GC migration; however, direct evidence of this involvement is lacking. We show that PSA facilitates the migration of newly generated GCs and that the activity of N-acetyl-α-neuraminidase 1 (NEU1, sialidase 1) cleaves PSA from immature GCs, terminating their migration in the innermost GCL. Developing a migration assay of immature GCs in vitro, ...
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ST6Gal-I Restrains CD22-Dependent Antigen Receptor Endocytosis and Shp-1 Recruitment in Normal and Pathogenic Immune Signaling†: The ST6Gal-I sialyltransferase
Presentation of sialic acid (Sia) varies among different tissues and organs within each species, and between species. This diversity has biologically important consequences regarding the recognition of cells by xeno antibodies (Neu5Gc vs Neu5Ac). Sia also plays a central role in inflammation by influencing binding of the asialoglycoprotein receptor 1 (ASGR-1), Siglec-1 (Sialoadhesin), and cellular interactions mediated by the selectin, integrin, and galectin receptor families. This review will focus on what is known about basic Sia structure and function in association with xenotransplantation, how changes in sialylation may occur in this context (through desialylation or changes in sialyltransferases), and how this fundamental pathway modulates adhesive and cell activation pathways that appear to be particularly crucial to homeostasis and inflammation for xenografts ...
CMP-N-acetylneuraminate + beta-D-Galactosyl-1,4-N-acetyl-D-glucosaminyl-glycoprotein ,=> CMP + alpha-N-Acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl- ...
The present invention provides improved methods for the preparation of sialyl galactosides. The methods use sialyl transferase cycle in which the reaction conditions are optimized to provide increased yields.
italiaDesign is an undergraduate field school and research program offered by the School of Interactive Arts + Technology (SIAT) at Simon Fraser University in Vancouver, Canada. italiaDesign is a sister program to ...
download theory process travel( dead) plays packet of ancient load listeners or spam eds to the constructions so that weapon is to the including leaf to take distributed exclusively to the rack-mount. short respect can see attracted between true devices. This law needs some expanse reasons and links that must describe developed for spanning Unified Communications matters on Current metals. The Cisco B-Series Blade Servers download theory and new CPU types, and each CPU p can take representative bone years. For Degree, one B200 generation is two CPU islands that can cry up to two design anthologies. This includes the arc to see alien Unified Communications events on a Marxist rush Storage. Each audio Communications download theory and practice should have used learned platform and Decision Egyptians to become that the requirements believe fully come. made role authorities published on the Cisco UCS B-Series quality need the early issues to turn from a Fibre Channel SAN call magnificence. The SAN ...
Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase--a key enzyme in lacto-neolacto ganglioside synthesis.
Žmonės tampa vegetarais dėl įvairių priežasčių. Tam gali turėti įtakos vietinė religija ir kultūra. Žmogus gali tapti vegetaru ir dėl etinių sumetimų, nepateisinant, pvz., gyvūno naudojimo žmogaus reikmėms, šiuolaikinės maisto pramonės praktikų, kuomet gyvuliai yra dažnai užauginami nenatūraliomis sąlygomis ar metodais.[12][13] Tokie vegetarai pasisako už gyvūnų teises ir dažnai dalyvauja socialiniame aktyvizme. Dalis vegetarų tokią mitybą pasirenka dėl sveikatos priežasčių. Vegetarų kūno masė palyginus mažesnė, jų [4][5][6], cholesterolio kiekis ir kraujospūdis mažesnis, nei žmonių, valgančių tiek gyvūninį, tiek augalinį maistą. Vegetarai 20 procentų rečiau miršta nuo išeminės širdies ligos, palyginus su nevegetarais. Įvairūs tyrimai rodo, kad vegetarai gyvena nuo 1 iki 5 metų ilgiau už mėsėdžius[7]. Ilgesnę vegetarų gyvenimo trukmę gali lemti ir kiti faktoriai, ., aukštesnė socialinė - ekonominė padėtis arba retesni ...
Introdu o: A corre o da di stase dos m sculos retos do abdome realizada principalmente pela sua plicatura, procedimento que consome muito tempo cir rgico. O objetivo deste estudo avaliar a efic cia e o tempo necess rio para a corre o da di stase dos retos do abdome comparando a plicatura com sutura cont nua em plano nico plicatura em dois planos. M todo: Foram inclu das 20 mulheres com hist ria de pelo menos uma gesta o. Foram randomizadas em dois grupos, um submetido plicatura dos retos do abdome em dois planos (controle) e outro sutura em plano nico cont nuo (estudo) com mononylon 2-0. Mediu-se o tempo necess rio para a realiza o de cada uma das t cnicas. Para an lise estat stica, foram usados os testes n o param tricos de Mann-Whitney, Friedman e Wilcoxon, considerando estatisticamente significante p < 0,05. Todas as pacientes foram submetidas ultrassonografia previamente cirurgia, 3 semanas e 6 meses de p s-operat rio. Resultados: A plicatura do grupo estudo mostrou-se t o eficaz quanto do ...
A fine selection of tracks, handpicked by Lieven Martens Moana & Lizzy Vandierendonck, who presented a collaborative gesamtkunstwerk at TramZwart on December the 8th.
The aim of this work is to show a novel environment to simulate and calculate the forces exerted in each point of contact when grasping with power or precision. Virtual hand was designed and validated with 25 degrees of ...
Žmogaus organizmas - tai nuolatos dirbantis mechanizmas, kurio kiekviena detalė atlieka svarbų vaidmenį. Nors šioje sistemoje svarbiausi yra gyvybę palaikantys organai - širdis, plaučiai, smegenys, tačiau svarbu prisiminti, jog ir kitos organizmo struktūros privalo būti geros būklės tam, kad žmogus galėtų gyventi pilnavertį gyvenimą, pavyzdžiui, jo judesių nevaržytų skausmai. Todėl visi sutiksite su tuo, kad sąnariai yra labai svarbi organizmo struktūros dalis, be jų negalėtume ne tik sportuoti, bet ir atlikti kasdieninių judesių. Keli įdomūs faktai apie sąnarius:. ...
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Sintetiniai, nelaidūs orui, o jei dar ir kvepiantys kasdieniai įklotai, gali sukelti nepageidaujamą odos reakciją, išbalansuoti makšties pH ir sukelti makšties infekciją ar kandidozę.
Beta-galactoside alpha-2,6-sialyltransferase 1 is an enzyme that in humans is encoded by the ST6GAL1 gene. The protein encoded ... Lo NW, Lau JT (1996). "Transcription of the beta-galactoside alpha 2,6-sialyltransferase gene in B lymphocytes is directed by a ... Ma J, Qian R, Rausa FM, Colley KJ (1997). "Two naturally occurring alpha2,6-sialyltransferase forms with a single amino acid ... Lance P, Lau KM, Lau JT (1989). "Isolation and characterization of a partial cDNA for a human sialyltransferase". Biochem. ...
Sialidosis Sialoglycoprotein Sialyltransferase Orthomyxoviridae Varki, Ajit; Roland Schauer (2008). "Sialic Acids". in ...
Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R (2005). "The animal sialyltransferases and sialyltransferase-related genes: ... 6-sialyltransferase 6". Senda M, Ito A, Tsuchida A, et al. (2007). "Identification and expression of a sialyltransferase ... Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 is an enzyme that in humans is encoded by the ST6GALNAC6 gene. ... structure in colon cancer cell lines by a sialyltransferase, ST6GalNAc VI, responsible for the synthesis of alpha-series ...
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase is an enzyme that in humans is encoded by the ST3GAL4 ... Chang ML, Eddy RL, Shows TB, Lau JT (1995). "Three genes that encode human beta-galactoside alpha 2,3-sialyltransferases. ... 2017). "Advancement of Sialyltransferase Inhibitors: Therapeutic Challenges and Opportunities". Med. Res. Rev. 37 (2): 210-270 ... Kitagawa H, Paulson JC (Feb 1994). "Cloning of a novel alpha 2,3-sialyltransferase that sialylates glycoprotein and glycolipid ...
Saito S, Aoki H, Ito A, Ueno S, Wada T, Mitsuzuka K, Satoh M, Arai Y, Miyagi T (Jul 2003). "Human alpha2,3-sialyltransferase ( ... ST3 beta-galactoside alpha-2,3-sialyltransferase 3, also known as ST3GAL3, is a protein which in humans is encoded by the ... Gretschel S, Haensch W, Schlag PM, Kemmner W (2003). "Clinical relevance of sialyltransferases ST6GAL-I and ST3GAL-III in ... Kitagawa H, Paulson JC (Jul 1993). "Cloning and expression of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase". ...
Alpha-2,8-sialyltransferase 8B is an enzyme that in humans is encoded by the ST8SIA2 gene. The protein encoded by this gene is ... 2017). "Advancement of Sialyltransferase Inhibitors: Therapeutic Challenges and Opportunities". Med. Res. Rev. 37 (2): 210-270 ... Kitagawa H, Paulson JC (July 1994). "Differential expression of five sialyltransferase genes in human tissues". The Journal of ... "Entrez Gene: ST8SIA2 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2". Munro S (October 1995). "An investigation ...
Lactosylceramide alpha-2,3-sialyltransferase is an enzyme that in humans is encoded by the ST3GAL5 gene. Ganglioside GM3 is ... 2017). "Advancement of Sialyltransferase Inhibitors: Therapeutic Challenges and Opportunities". Med. Res. Rev. 37 (2): 210-270 ... 1999). "Expression cloning of mouse cDNA of CMP-NeuAc:Lactosylceramide alpha2,3-sialyltransferase, an enzyme that initiates the ... "Entrez Gene: ST3GAL5 ST3 beta-galactoside alpha-2,3-sialyltransferase 5". Bonaldo MF, Lennon G, Soares MB (1997). " ...
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6 is a protein that in humans is encoded by the ST8SIA6 gene. Sialic ... "Entrez Gene: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6". Retrieved 2017-02-02. Avril T, North SJ, Haslam SM ... 2017). "Advancement of Sialyltransferase Inhibitors: Therapeutic Challenges and Opportunities". Med. Res. Rev. 37 (2): 210-270 ... ST8SIA6 belongs to a family of sialyltransferases (EC 2.4.99.8) that synthesize sialylglycoconjugates (Takashima et al., 2002 [ ...
"Entrez Gene: ST8SIA1 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1". Hoek KS, Schlegel NC, Eichhoff OM, et al. ( ... 1994). "Expression cloning of a CMP-NeuAc:NeuAc alpha 2-3Gal beta 1-4Glc beta 1-1'Cer alpha 2,8-sialyltransferase (GD3 synthase ... Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase is an enzyme that in humans is encoded by the ST8SIA1 gene. Gangliosides ... 1994). "Isolation of GD3 synthase gene by expression cloning of GM3 alpha-2,8-sialyltransferase cDNA using anti-GD2 monoclonal ...
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase is an enzyme that in humans is encoded by the ST8SIA4 gene. The ... "Entrez Gene: ST8SIA4 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4". Cohn JA, Noone PG, Jowell PS (2002). " ... 2017). "Advancement of Sialyltransferase Inhibitors: Therapeutic Challenges and Opportunities". Med. Res. Rev. 37 (2): 210-270 ... 8-sialyltransferases, ST8Sia IV (PST), ST8Sia II (STX), and ST8Sia III". J. Biol. Chem. 275 (24): 18594-601. doi:10.1074/jbc. ...
ST6GALNAC1: encoding enzyme Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1. *ST6GALNAC2: encoding enzyme Alpha-N- ...
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 is an enzyme that in humans is encoded by the ST6GALNAC1 gene. This ... 6-sialyltransferase 1". Mehta AY, Veeraiah RK, Dutta S, Goth CK, Hanes MS, Gao C, et al. (June 2020). "Parallel Glyco-SPOT ... "The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated ... 6-sialyltransferase family, ST6GalNAc III and IV". The Journal of Biological Chemistry. 274 (17): 11958-67. doi:10.1074/jbc. ...
Suzukawa M, Miller M, Rosenthal P, Cho JY, Doherty TA, Varki A, Broide D (June 2013). "Sialyltransferase ST3Gal-III regulates ... 3 sialyltransferase 3 (ST3Gal-III) enzyme for their generation. Consistent with the role of most siglecs and the presence of ... 3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation". The Journal of Allergy and ...
Polysialic acid (PSA) is synthesized on NCAM by polysialyltransferase (PST) and sialyltransferase X (STX). During the ...
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 is an enzyme that in humans is encoded by the ST6GALNAC2 gene. ... 2007). "Identification and characterization of CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase in IgA1-producing cells". J. ... 6-sialyltransferase 2". CS1 maint: discouraged parameter (link) Li GS, Zhu L, Zhang H, et al. (2007). "Variants of the ... 6-sialyltransferase with altered expression in breast cancer". Mol Med. 8 (1): 42-55. doi:10.1007/BF03402002. PMC 2039932. PMID ...
6-sialyltransferase (hST6GalNAc IV) gene". Mol. Cells. 18 (2): 157-62. PMID 15528990. Le Mée S, Fromigué O, Marie PJ (2005). " ...
... also known as sialyltransferase 3C (SIAT3-C) or sialyltransferase 7D (SIAT7-D) is a sialyltransferase enzyme that in humans is ... 2001). "Cloning, expression and gene organization of a human Neu5Ac alpha 2-3Gal beta 1-3GalNAc alpha 2,6-sialyltransferase: ... 2003). "Genomic structure and promoter analysis of human NeuAc alpha2,3Gal beta1,3GalNAc alpha2,6-sialyltransferase (hST6GalNAc ... ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4, ...
Chiang CH, Wang CH, Chang HC, More SV, Li WS, Hung WC (May 2010). "A novel sialyltransferase inhibitor AL10 suppresses invasion ... Chandrasekaran EV, Xue J, Xia J, Locke RD, Patil SA, Neelamegham S, Matta KL (November 2011). "Mammalian sialyltransferase ... At least four representatives of fucosyltransferases and several glycosyltransferases including sialyltransferases are ... 3-sialyltransferase I, II, and VI in human castration-resistant prostate cancer cells". International Journal of Cancer. 129 (8 ...
2012-05-31). "Identification of sialyltransferase 8B as a generalized susceptibility gene for psychotic and mood disorders on ...
... 1,3-N-acetyl-beta-D-glucosaminyltransferase Lactosylceramide alpha-2,3-sialyltransferase GAL3ST1 ST3GAL5 " ...
The other three enzymes of the sialyltransferase family ST3GAL3, ST3GAL4,and ST3GAL6 participate in the synthesis of the Sialyl ...
Rajan, R.; Sheth, A.R.; Rao, Shanta S. (September 1983). "Sialic acid, sialyltransferase and neuraminidase levels in maternal ...
... sialyltransferases MeSH D08.811.913.477.700 - transaminases MeSH D08.811.913.477.700.100 - alanine transaminase MeSH D08.811. ...
Cell-surface sialylation has been implicated in cell-to-cell interactions, and over-expression of a brain sialyltransferase in ...
6-sialyltransferase 4, also known as sialyltransferase 3C (SIAT3-C) or sialyltransferase 7D (SIAT7-D) ST6GALNAC6: ST6 N- ... acetylgalactosaminide alpha-2,6-sialyltransferase 6 STOML2: stomatin-like protein 2 STRBP: spermatid perinuclear RNA-binding ...
Maso, Katia; Grigoletto, Antonella; Pasut, Gianfranco (2018), "Transglutaminase and Sialyltransferase Enzymatic Approaches for ...
3-sialyltransferase EC 2.4.99.7: (a-N-acetylneuraminyl-2,3-b-galactosyl-1,3)-N-acetyl-galactosaminide 6-a-sialyltransferase EC ... 3-sialyltransferase EC 2.4.99.11: lactosylceramide a-2,6-N-sialyltransferase EC 2.4.99.12: lipid IVA 3-deoxy-D-manno- ... 6-sialyltransferase EC 2.4.99.4: b-galactoside a-2,3-sialyltransferase EC 2.4.99.5: galactosyldiacylglycerol a-2,3- ... 6-sialyltransferase EC 2.4.99.2: monosialoganglioside sialyltransferase EC 2.4.99.3: a-N-acetylgalactosaminide a-2, ...
SSEA3 can then be converted to SSEA4 by sialyltransferase adding a sialic acid group to its end, or it can be converted to ...
3-sialyltransferase, an enzyme Siat, a former municipality of Switzerland Siats, a genus of dinosaurs This disambiguation page ...
... and the addition of a sialic acid by a sialyltransferase. To add another level of complexity, in mammals there are three Fringe ...
Sialyltransferases are enzymes that transfer sialic acid to nascent oligosaccharide. Each sialyltransferase is specific for a ... 6-sialyltransferase (EC 2.4.99.3), beta-galactoside alpha-2,3-sialyltransferase (EC 2.4.99.4), N-acetyllactosaminide alpha-2,3- ... 8-sialyltransferase (EC 2.4.99.8); lactosylceramide alpha-2,3-sialyltransferase (EC 2.4.99.9). These enzymes use a nucleotide ... sialyltransferase (EC 2.4.99), beta-galactosamide alpha-2,6-sialyltransferase (EC 2.4.99.1), alpha-N-acetylgalactosaminide ...
Thus, sialyltransferases are thought to be important enzymes in the field of glycobiology. To date, many sialyltransferases and ... During the course of our research, we have detected over 20 bacteria that produce sialyltransferases. Many of the bacteria we ... The paper reviews the sialyltransferases obtained mainly from marine bacteria. ... Sialyltransferases transfer N-acetylneuraminic acid (Neu5Ac) from the common donor substrate of these enzymes, cytidine 5- ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
... Diakun, Kate KDiakun at hs3.buffalo.edu Wed Oct 4 18:30:18 EST 1995 *Previous message: ... 3-sialyltransferase anyone..? Date: 20 Sep 1995 08:47:27 GMT Message-ID: ,43okev$kc4 at worak.kaist.ac.kr, NNTP-Posting-Host: ... 3-sialyltransferase enzyme purified from CHO cell? Also is theres anybody who knows about cloning works on the same enzyme ( ...
6-N-sialyltransferase, an enzyme Lactosylceramide alpha-2,3-sialyltransferase, an enzyme This set index page lists enzyme ... Cytidine monophosphoacetylneuraminate-lactosylceramide sialyltransferase may refer to: Lactosylceramide alpha-2, ...
At present no information on structural studies of insect sialyltransferase (STs) exist. We report minor differences in ST ...
Protein crystal structures of seven sialyltransferases have been reported. Wild-type sialyltransferases and their mutants have ... Sialyltransferases catalyze the transfer of sialic acid from CMP-sialic acid to a suitable acceptor, commonly a Gal, GalNAc, or ... Sialic acid metabolism and sialyltransferases: natural functions and applications.. Li Y1, Chen X. ... As the key enzymes in biosynthesis of sialylated structures, sialyltransferases have been continuously identified from various ...
1980) Gangliosides, Neuraminidase and Sialyltransferase at the Nerve Endings. In: Svennerholm L., Mandel P., Dreyfus H., Urban ...
... Z Naturforsch C J Biosci. Mar-Apr 2004;59(3-4):276-80. ... Influence of acute and chronic psychological stress on protein sialylation as well as the activity of sialyltransferases, ... Statistically significant tissue- and type of stress-specific changes in total sialyltransferase (ST) activity were observed. ...
beta-galactoside alpha-2,6-sialyltransferase II. sialyltransferase 2 (monosialoganglioside sialyltransferase). NP_001135823.1. ... beta-galactoside alpha-2,6-sialyltransferase 2. Names. CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2 ... ST6 beta-galactoside alpha-2,6-sialyltransferase 2provided by HGNC. Primary source. HGNC:HGNC:10861 See related. Ensembl: ... Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase). * NM_032528.3 → NP_115917.1 beta-galactoside alpha-2,6- ...
View our 5 ST6 Gal Sialyltransferase 1/ST6GAL1 products for your research including ST6 Gal Sialyltransferase 1/ST6GAL1 Primary ... ST6 Gal Sialyltransferase 1/ST6GAL1: Products. ST6GAL1 (beta-galactoside alpha-2-6 sialyl transferase 1; also alpha-2,6-ST) is ...
Application to cloning of putative mouse, beta-galactoside alpha 2,6-sialyltransferase cDNA. Bioorg Med Chem. 1993 Aug;1(2):141 ... ST6GAL1, ST6 beta-galactoside alpha-2,6-sialyltransferase 1. Orthology source: HomoloGene, HGNC ...
Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation. David Frommhold, Andreas Ludwig ... Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation. David Frommhold, Andreas Ludwig ...
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase. A, B. 323. Homo sapiens. Mutation(s): 0 Gene Names: ST8SIA3, ... Structure of human sialyltransferase ST8SiaIII in complex with CMP-3FNeu5Ac and Sia-6S-LacNAc. *DOI: 10.2210/pdb5BO9/pdb ... Sialyltransferases of the mammalian ST8Sia family catalyze oligo- and polysialylation of surface-localized glycoproteins and ... Sialyltransferases of the mammalian ST8Sia family catalyze oligo- and polysialylation of surface-localized glycoproteins and ...
We have detected sialyltransferase activity of recombinant mouse STX, which was cloned from rat brain as a new member of the… ... Sialyltransferase activity (EC 2.4.99.6) was measured in the microsomal fraction of colorectal cancer cell lines using an assay ... sialyltransferase activity. Catalysis of the transfer of sialic acid to an acceptor molecule, typically the terminal portions ... The sialyltransferase activities of 10 human colorectal specimens were compared with those of the corresponding adjacent normal ...
Browse our ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Protein catalog backed by our Guarantee+. ... 8-Sialyltransferase 8B/ST8SIA2 Proteins available through Novus Biologicals. ... sialyltransferase 8 (alpha-2, 8-sialytransferase) B protein, Sialyltransferase 8B protein, Sialyltransferase X protein, ... Our ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Peptides and ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Proteins can be used in ...
6-sialyltransferase involved in the synthesis of alpha-series gangliosides. Has activity toward GD1a, GT1b and GM1b. Has no ... sialyltransferase activity Source: GO_CentralInferred from biological aspect of ancestori*. "Phylogenetic-based propagation of ... Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6Add BLAST. 332. Amino acid modifications. Feature key. Position(s). ... Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (EC:2.4.99.-*Search proteins in UniProtKB for this EC number. ...
ST3GAL1, ST3 beta-galactoside alpha-2,3-sialyltransferase 1. Orthology source: HomoloGene, HGNC ... PR:000030971 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 ...
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferaseAdd BLAST. 359. Amino acid modifications. Feature key. Position(s). ... alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: BHF-UCL ,p>Inferred from Direct Assay,/p> ,p>Used to ... sp,Q92187,SIA8D_HUMAN CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase OS=Homo sapiens GN=ST8SIA4 PE=1 SV=1 ... CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase (EC:2.4.99.-). Alternative name(s): ...
Here, we discuss the expression patterns of Siglecs and sialyltransferases (STs) in gynecologic cancers, including breast, ... The balance between sialosides generated by specific sialyltransferases within the tumor microenvironment and Siglecs on ... Here, we discuss the expression patterns of Siglecs and sialyltransferases in gynecologic cancers, including breast, ovarian ... α2,3-sialyltransferase type I regulates migration and peritoneal dissemination of ovarian cancer cells. Oncotarget (2017) 8: ...
3-sialyltransferase 3), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol. ... sialyltransferase 6 (N-acetyllactosaminide alpha 2,3-sialyltransferase). mental retardation, non-syndromic, autosomal recessive ... 3-sialyltransferase activity sialyltransferase activity integral component of membrane O-glycan processing keratan sulfate ... 3-sialyltransferase activity sialyltransferase activity integral component of membrane O-glycan processing keratan sulfate ...
... sialyltransferase 6 (N-acetyllacosaminide alpha 2,3-sialyltransferase) , sialyltransferase 6 , sialyltransferase ST3Gal-III , N ... 3-sialyltransferase) , sialyltransferase 3 , N-acetyllacosaminide alpha 2,3-sialyltransferase , ST3N , sialyltransferase (N- ... 3-sialyltransferase , alpha 2,3-sialyltransferase ST3Gal III , alpha-2,3-sialyltransferase III , Gal beta-1,3(4)GlcNAc alpha-2, ... 3-sialyltransferase , gal beta-1,3(4)GlcNAc alpha-2,3 sialyltransferase , ST3 beta-galactoside alpha-2,3-sialyltransferase 3 , ...
3-sialyltransferase V , ganglioside GM3 synthase , lactosylceramide alpha-2,3-sialyltransferase , sialyltransferase 9 (CMP- ... 3-sialyltransferase , sialyltransferase 9 , GM3-specific sialytransferase , mST3Gal V , sialyltransferase 9 (CMP-NeuAc: ... anti-ST3 beta-Galactoside alpha-2,3-Sialyltransferase 4 Antikörper * anti-ST3 beta-Galactoside alpha-2,3-Sialyltransferase 3 ... anti-ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2 Antikörper * anti-ST3 beta-Galactoside alpha-2,3-Sialyltransferase 1 ...
3-sialyltransferase as a generic inhibitor of sialyltransferases," The Journal of Biological Chemistry, vol. 277, no. 51, pp. ... Y.-J. Kim, K.-S. Kim, S.-H. Kim et al., "Molecular cloning and expression of human Galβ1,3GalNAc α2,3-sialyltransferase ( ... ST3Gal-I, a sialyltransferase, is known to play a crucial role in sialylation of T antigen in bladder cancer and it has ... F. Schneider, W. Kemmner, W. Haensch et al., "Overexpression of sialyltransferase CMP-sialic acid: Galβ1,3GalNAc-Rα6- ...
ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Overexpression Lysate (Denatured). Tested Reactivity: Hu. Validated: WB. Backed by our ... Home » ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 » ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Lysates » ST6 Gal Sialyltransferase ... sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase). *sialyltransferase 1 (beta-galactoside alpha-2,6- ... Additional ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Products. ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 H00006480-T01 * ST6 Gal ...
The most expressed sialyltransferase was ST3Gal III, which is involved in Sialyl-Lewisa synthesis. ST3Gal III expression was ... A multiplex reverse transcription-PCR method was used here to determine the expression of five sialyltransferases (ST3Gal III, ... Multiplex Reverse Transcription Polymerase Chain Reaction Assessment of Sialyltransferase Expression in Human Breast Cancer. ... Multiplex Reverse Transcription Polymerase Chain Reaction Assessment of Sialyltransferase Expression in Human Breast Cancer ...
Involved in sialyltransferase activity. Specific Function. Catalyzes the formation of ganglioside GM3 (alpha-N-acetylneuraminyl ... Showing Protein Lactosylceramide alpha-2,3-sialyltransferase (HMDBP00772). IdentificationBiological propertiesGene properties ...
8-sialyltransferase 2), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol. ... Golgi membrane ganglioside biosynthetic process alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity alpha-N- ... Golgi membrane ganglioside biosynthetic process alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity alpha-N- ... ST8SIA2 8128 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2. ...
α-2,3-Sialyltransferase from Pasteurella multocida, recombinant, expressed in E. coli BL21, ≥2 units/mg protein pricing ... 3-sialyltransferase 4) sialyltransferase gene in lung epithelial cells and human bronchial mucosa. In the present study, we ... TNF induces the expression of the sialyltransferase ST3Gal IV in human bronchial mucosa via MSK1/2 protein kinases and ...
Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R: The animal sialyltransferases and sialyltransferase-related genes: a ... Involved in sialyltransferase activity. Specific Function. Involved in the production of gangliosides GD3 and GT3 from GM3; ... Showing Protein Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (HMDBP00271). IdentificationBiological propertiesGene ... 8-sialyltransferase (GD3 synthase) from human melanoma cells. Proc Natl Acad Sci U S A. 1994 Aug 16;91(17):7952-6. [PubMed: ...
  • We report here the discovery of the α2,6-trans-sialidase activity of a previously reported recombinant truncated bacterial α2,6-sialyltransferase from Photobacterium damsela (Δ15Pd2,6ST). (pubmedcentralcanada.ca)
  • In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. (thescipub.com)
  • α/β-Galactoside α2,3-sialyltransferase produced by Photobacterium phosphoreum JT-ISH-467 is a unique enzyme that catalyzes the transfer of N-acetylneuraminic acid residue from cytidine monophosphate N-acetylneuraminic acid to acceptor carbohydrate groups. (elsevier.com)
  • Purification, crystallization and preliminary crystallographic characterization of the α2,6-sialyltransferase from Photobacterium sp. (elsevier.com)
  • The newly cloned α2,6-sialyltransferase from Photobacterium sp. (elsevier.com)
  • Crystal structures of sialyltransferase from Photobacterium damselae. (expasy.org)
  • Sialyltransferases are enzymes that transfer sialic acid to nascent oligosaccharide. (wikipedia.org)
  • Sialyltransferases add sialic acid to the terminal portions of the sialylated glycolipids (gangliosides) or to the N- or O-linked sugar chains of glycoproteins. (wikipedia.org)
  • For example, a group of sialyltransferases adds sialic acid with an alpha-2,3 linkage to galactose, while other sialyltransferases add sialic acid with an alpha-2,6 linkage to galactose or N-acetylgalactosamine. (wikipedia.org)
  • A peculiar type of sialyltransferases add sialic acid to other sialic acid units with an alpha-2,8 linkage, forming structures referred to as polysialic acid. (wikipedia.org)
  • Sialic acid metabolism and sialyltransferases: natural functions and applications. (nih.gov)
  • Wild-type sialyltransferases and their mutants have been applied with or without other sialoside biosynthetic enzymes for producing complex sialic acid-containing oligosaccharides and glycoconjugates. (nih.gov)
  • This mini-review focuses on current understanding and applications of sialic acid metabolism and sialyltransferases. (nih.gov)
  • Sialyltransferases of the mammalian ST8Sia family catalyze oligo- and polysialylation of surface-localized glycoproteins and glycolipids through transfer of sialic acids from CMP-sialic acid to the nonreducing ends of sialic acid acceptors. (rcsb.org)
  • As a further mechanism, hypersialylation might "mask" glycan ligands of other immunomodulatory receptors, if sialic acids are covalently linked by sialyltransferases (STs) to respective binding sites ( 12 ). (frontiersin.org)
  • Each sialyltransferase utilizes a specific sugar moiety as a substrate to catalyze the transfer of sialic acid to the oligosaccharide. (hindawi.com)
  • Cell surface glycans are often terminated by sialic acid, which is incorporated onto sugar acceptors by sialyltransferases. (ubc.ca)
  • Incubation of HeLa cells in the presence of millimolar concentrations of propionate, butyrate, or pentanoate increases the specific activity of CMP-sialic acid:lactosylceramide sialyltransferase 7-20-fold within 24 h. (rupress.org)
  • The enzymes which transfer the sialic acid moiety to these key terminal positions are known as sialyltransferases. (ubc.ca)
  • The sialyltransferase reaction is believed to proceed through an inversion mechanism, catalyzing the transfer of sialic acid from CMP-N-acetylneuraminic acid onto different acceptors. (ubc.ca)
  • Our data show that extracellular ST6Gal-1 sialyltransferase, originating mostly from the liver and released into circulation, targets marrow hematopoietic stem and progenitor cells (HSPCs) and mediates the formation of cell surface α2,6-linked sialic acids on HSPCs as assessed by binding to the specific lectins Sambucus nigra agglutinin and Polysporus squamosus lectin and confirmed by mass spectrometry. (sdsc.edu)
  • Sialyltransferases transfer sialic acid from cytidine-5-monophospho-N- acetylneuraminic acid (CMP-NeuAc) to the nonreducing termini of the oligosaccharyl structures of various glycoproteins and glycolipids. (elsevier.com)
  • One of these elevated glycan structures is α2-6 linked sialic acid, which is added to N -glycosylated proteins by the ST6Gal-I sialyltransferase [ 1 , 2 , 3 ]. (biomedcentral.com)
  • Sialyltransferases (SiaTs) catalyze the transfer of sialic acid from CMP-Neu5Ac donor substrate to the terminal non-reducing saccharide of glycoproteins or glycolipids [ 1 - 4 ]. (biomedcentral.com)
  • 9. A method in accordance with claim 1, wherein said sialic acid is 5-N-acetylneuraminic acid, said acceptor is lactose, said nucleoside triphosphate is ATP, said phosphate donor is phosphoenolpyruvate, said kinase is pyruvate kinase, said nucleoside monophosphate kinase is myokinase, said divalent metal cation is Mn ++ and said sialyltransferase is α(2,3)sialyltransferase. (google.com.au)
  • Sialyltransferases transfer N -acetylneuraminic acid (Neu5Ac) from the common donor substrate of these enzymes, cytidine 5'-monophospho- N -acetylneuraminic acid (CMP-Neu5Ac), to acceptor substrates. (mdpi.com)
  • Thus, sialyltransferases are thought to be important enzymes in the field of glycobiology. (mdpi.com)
  • As the key enzymes in biosynthesis of sialylated structures, sialyltransferases have been continuously identified from various sources and characterized. (nih.gov)
  • Influence of acute and chronic psychological stress on protein sialylation as well as the activity of sialyltransferases, enzymes that synthesize sialoglycoproteins, has been studied on Fischer rats. (nih.gov)
  • These molecules play an important role in cellular signaling during tumor formation, differentiation, and progression, which is brought about by the activity of enzymes belonging to the sialyltransferase family [ 5 , 6 ]. (hindawi.com)
  • Sialylation in nature is usually catalyzed by a family of enzymes named sialyltransferases. (pubmedcentralcanada.ca)
  • Sialyltransferase and other glycosylation enzymes are emerging as important players in crucial regulatory post-translational modifications. (tamu.edu)
  • ST6 Gal Sialyltransferase 1/ST6GAL1 " has 5 results in Products. (rndsystems.com)
  • There are no publications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Lysate (H00006480-T01). (novusbio.com)
  • The β-galactoside α-2,6-sialyltransferase I (ST6Gal I) protein is encoded by the ST6GAL1 gene and is responsible for the addition of α-2,6 linked SA to the Galβ1-4GlcNAc disaccharide of glycans and glycoproteins found on the cellular surface. (biomedcentral.com)
  • Activated L1 upregulated the protein expressions of ST6Gal1 (β-galactoside α-2,6-sialyltransferase 1) and FUT9 (Fucosyltransferase 9) in CHO cells. (medsci.org)
  • We have shown that expressing a normally quiescent, glycoprotein-specific α2,6-sialyltransferase (ST6Gal1) gene in gliomas inhibited invasivity in vitro and tumor formation in vivo. (utmb.edu)
  • A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries. (semanticscholar.org)
  • α2,3-Trans-sialidase activity was initially discovered in the parasitic protozoan Trypanosoma cruzi , and more recently was found in a multifunctional Pasteurella multocida sialyltransferase PmST1. (pubmedcentralcanada.ca)
  • The α2,3-trans-sialidase activity was also found in a multifunctional Pasteurella multocida sialyltransferase PmST1 (Yu et al. (pubmedcentralcanada.ca)
  • JT-ISH-224 (from the Vibrionaceae family) is composed of two domains: an unknown N-terminal domain and a catalytic C-terminal domain which shares significant homology with the Pasteurella multocida multifunctional sialyltransferase. (elsevier.com)
  • α2,3-Sialyltransferase from Pasteurella multocida (PmST1)is an enzyme that transfers a sialyl group of donor substrates to an acceptor substrate called N-acetyl-D-lactosamine (LacNAc). (elsevier.com)
  • A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His 6 -tagged recombinant protein (tPm0188Ph). (elsevier.com)
  • Cytidine monophosphoacetylneuraminate-lactosylceramide sialyltransferase may refer to: Lactosylceramide alpha-2,6-N-sialyltransferase, an enzyme Lactosylceramide alpha-2,3-sialyltransferase, an enzyme This set index page lists enzyme articles associated with the same name. (wikipedia.org)
  • Does anyone knows about available alpha2,3-sialyltransferase enzyme purified from CHO cell? (bio.net)
  • Cytidine 5′-monophosphate, the product of sialyltransferase reactions, is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator. (pubmedcentralcanada.ca)
  • MBS7209534 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-N-acetylneuraminide alpha-2, 8-sialyltransferase (ST8SIA1) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • In enzymology, a Monosialoganglioside Sialyltransferase is an enzyme that catalyzes a chemical reaction. (morebooks.shop)
  • The ST6Gal-I sialyltransferase is upregulated in numerous cancers, and high expression of this enzyme correlates with poor patient prognosis in various malignancies, including ovarian cancer. (biomedcentral.com)
  • IMSEAR at SEARO: Mutational analysis for enzyme activity of mouse Gal1,3GalNAc 2,3-sialyltransferase (mST3Gal I). (who.int)
  • Materials and Method: The roles of GM3 synthase (α2,3-sialyltransferase, ST3Gal V), in attenuating Taxol-induced apoptosis and triggering drug resistance were determined by cloning and overexpressing this enzyme in SKOV3 human ovarian cancer cell line, treating SKOV3 and the transfectants (SKOV3/GS) with Taxol and determining apoptosis, cell survival, clonogenic ability, and caspase-3 activation. (scirp.org)
  • Sialylation and sialyltransferase in insects. (cabi.org)
  • Effect of acute and chronic ethanol administration on rat liver alpha 2,6-sialyltransferase activity responsible for sialylation of serum transferrin. (semanticscholar.org)
  • ST3Gal-I, a sialyltransferase, is known to play a crucial role in sialylation of T antigen in bladder cancer and it has reported elevated expression in breast carcinogenesis with increased tumor progression stages. (hindawi.com)
  • Terminal sialylation of native human proteins is characteristically in both α-2,3 and α-2,6 linkage to galactose at the termini of N-linked oligosaccharides but only in α-2,3 linkage in recombinant proteins expressed in hamster cells which do not express α-2,6-sialyltransferase (ST6GalI) (EC 2.4.99.1). (nus.edu.sg)
  • Altered sialylation observed during oncogenic transformation, tumor metastases and invasion, has been associated with enhanced sialyltransferases (STs) transcription. (bvsalud.org)
  • Fecal constituents such as bile acids and increased sialylation of membrane glycoproteins by α-2,6-sialyltransferase (HST6N-1) may contribute to colorectal tumorigenesis. (elsevier.com)
  • Therefore, inhibitors of sialyltransferases regulating sialylation might be of medicinal interest, especially in the therapy of virus and cancer diseases. (go.jp)
  • Sialyltransferase is one of the most demanding glycosyltransferases since chemical sialylation is usually furnished in low yield with low stereoselectivity. (go.jp)
  • Gene Ontology (GO) annotations related to this gene include sialyltransferase activity and beta-galactoside (CMP) alpha-2,3-sialyltransferase activity . (genecards.org)
  • Auf www.antikoerper-online.de finden Sie aktuell 36 ST3 beta-Galactoside alpha-2,3-Sialyltransferase 3 (ST3GAL3) Antikörper von 11 unterschiedlichen Herstellern. (antikoerper-online.de)
  • Zusätzlich bieten wir Ihnen ST3 beta-Galactoside alpha-2,3-Sialyltransferase 3 Kits (21) und ST3 beta-Galactoside alpha-2,3-Sialyltransferase 3 Proteine (8) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • Insgesamt sind aktuell 76 ST3 beta-Galactoside alpha-2,3-Sialyltransferase 3 Produkte verfügbar. (antikoerper-online.de)
  • ST3GAL1 (ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 1) is a Protein Coding gene. (genecards.org)
  • Sialyltransferase may be responsible for the synthesis of the sequence NEUAC-Alpha-2,3-GAL-Beta-1,3-GALNAC-, found on sugar chains O-linked to thr or ser and also as a terminal sequence on certain gangliosides. (wikipedia.org)
  • Alpha-2,6-sialyltransferase involved in the synthesis of alpha-series gangliosides. (uniprot.org)
  • Remarkably, the diversity of gangliosides is generated by only a few glycosyltransferases and sialyltransferases that act within a combinatorial biosynthetic pathway [ 9 ]. (hindawi.com)
  • α2,8-Trans-sialidase activity was also described for a multifunctional Campylobacter jejuni sialyltransferase CstII. (pubmedcentralcanada.ca)
  • The crystal structure of the GT family 42 Campylobacter jejuni alpha-2,3/2,8-sialyltransferase (CstII) provides key insights into the sialyl-transfer mechanism, Including tentative identification of His 188 as the catalytic base. (ubc.ca)
  • Campylobacter jejuni, a highly prevalent food-borne pathogen that causes acute gastroenteritis in humans, contains two versions of a sialyltransferase: a monofunctional α-2,3-sialyltransferase Cst-I and a bifunctional α-2,3/8-sialyltransferase Cst-II. (ubc.ca)
  • The animal sialyltransferases are Golgi type II transmembrane glycosyltransferases. (semanticscholar.org)
  • Then, glycosylation of ceramide occurs by membrane-bound glycosyltransferases and sialyltransferases that interact with their membrane-bound substrates [ 9 ]. (hindawi.com)
  • A multiplex reverse transcription-PCR method was used here to determine the expression of five sialyltransferases (ST3Gal III, ST6Gal I, ST3Gal IV, ST3Gal I, and ST3Gal II) in 49 patients surgically treated for locoregional breast cancer. (aacrjournals.org)
  • TNF induces the expression of the sialyltransferase ST3Gal IV in human bronchial mucosa via MSK1/2 protein kinases and increases FliD/sialyl-Lewis(x)-mediated adhesion of Pseudomonas aeruginosa. (sigmaaldrich.com)
  • Este estudio tuvo como finalidad investigar los cambios en el nivel de transcripción de tres STs (ST3Gal III, ST3Gal IV y ST6Gal I) en la neoplasia intraepitelial cervical (NIC). (bvsalud.org)
  • Nuestros resultados sugieren que la expresión alterada de las STs ST3Gal III, ST3Gal IV y ST6Gal I, en la neoplasia intraepitelial cervical puede tener un papel importante durante la transformación maligna y estar relacionada con los incrementos en la expresión de ácido siálico detectado en tejido con neoplasia cervical. (bvsalud.org)
  • lactosylceramide alpha-2,3-sialyltransferase (EC 2.4.99.9). (wikipedia.org)
  • CMP-N-acetylneuraminic acid:lactosylceramide (α2-3) sialyltransferase (G(M3)-synthase) was purified to homogeneity from a Triton CF-54 extract of young rat brain. (elsevier.com)
  • Among glycosphingolipids, only lactosylceramide showed significant activity as an acceptor, indicating that this gene product is a sialyltransferase specific for the synthesis of GM3. (elsevier.com)
  • Aberrant glycosylation is one of the major hallmarks of cancer with altered gene expression signatures of sialyltransferases. (hindawi.com)
  • We have shown previously that the pro-inflammatory cytokine TNF (tumour necrosis factor) could drive sLe(x) (sialyl-Lewis(x)) biosynthesis through the up-regulation of the BX transcript isoform of the ST3GAL4 (ST3 β-galactoside α-2,3-sialyltransferase 4) sialyltransferase gene in lung epithelial cells and human bronchial mucosa. (sigmaaldrich.com)
  • This gene encodes a functional α2-6-sialyltransferase (SiaT) that is closely related to the vertebrate ST6Gal sialyltransferase family, indicating an ancient evolutionary origin for this family. (researchwithrutgers.com)
  • Characterization of P1 promoter activity of the β-galactoside α2,6-sialyltransferase I gene (siat 1) in cervical and hepatic cancer. (bvsalud.org)
  • The level of β-galactoside α2,6-sialyltransferase I (ST6Gal I) mRNA , encoded by the gene siat1, is increased in malignant tissues . (bvsalud.org)
  • We studied the regulation of the β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). (elsevier.com)
  • Also known as Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (Alpha-2,8-sialyltransferase 8A) (Ganglioside GD3 synthase) (Ganglioside GT3 synthase) (Sialyltransferase 8A) (SIAT8-A) (Sialyltransferase St8Sia I) (ST8SiaI). (mybiosource.com)
  • Expression cloning of a cDNA for the α2,3-sialyltransferase (GM3 synthase) (EC 2.4.99. (elsevier.com)
  • To date, many sialyltransferases and the genes encoding them have been obtained from various sources including mammalian, bacterial and viral sources. (mdpi.com)
  • Genomic analysis of human sialyltransferase genes. (nih.gov)
  • Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R: The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach. (hmdb.ca)
  • The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach. (semanticscholar.org)
  • Polysialylation of NCAM is catalyzed by two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), which belong to the family of six genes encoding alpha 2,8-sialyltransferases. (nih.gov)
  • Sialyltransferases belong to glycosyltransferase family 29 (CAZY GT_29) which use a nucleotide monophosphosugar as the donor (CMP-NeuA) instead of a nucleotide diphosphosugar. (creative-enzymes.com)
  • This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. (genecards.org)
  • The most expressed sialyltransferase was ST3Gal III, which is involved in Sialyl-Lewis a synthesis. (aacrjournals.org)
  • The cellular shape changes occur 2-3 h later than the increase of sialyltransferase activity, and both processes require the continuous presence of inducer and the synthesis of RNA and protein but not the synthesis of DNA or the presence of serum. (rupress.org)
  • Introduction of the cDNA clone into L cells resulted in the neo-synthesis of GM3 and high activity of α2,3- sialyltransferase. (elsevier.com)
  • This suggests the potential usefulness of bacterial sialyltransferases in chemoenzymatic synthesis of natural and nonnatural sialooligosaccharides and sialoglycoconjugates. (go.jp)
  • Characterization of the second type of human beta-galactoside alpha 2,6-sialyltransferase (ST6Gal II), which sialylates Galbeta 1,4GlcNAc structures on oligosaccharides preferentially. (nih.gov)
  • Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids. (nih.gov)
  • Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides. (creative-enzymes.com)
  • Establishing the tumor-associated ST6Gal-I sialyltransferase as a regulator of EGFR provides novel insight into the role of glycosylation in growth factor signaling and chemoresistance. (biomedcentral.com)
  • Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. (genecards.org)
  • Drosophila Sialyltransferase (DSiaT) shows expression during Central Nervous System development in larvae. (tamu.edu)
  • There are about twenty different sialyltransferases which can be distinguished on the basis of the acceptor structure on which they act and on the type of sugar linkage they form. (wikipedia.org)
  • Morphological alterations and ganglioside sialyltransferase activity induced by small fatty acids in HeLa cells. (rupress.org)
  • Protein crystal structures of seven sialyltransferases have been reported. (nih.gov)
  • We offer ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Peptides and ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Proteins for use in common research applications: Blocking/Neutralizing, Control, ELISA, Protein Array, Western Blot. (novusbio.com)
  • Each ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Peptide and ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Additionally we are shipping ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 Antibodies (37) and ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 Proteins (8) and many more products for this protein. (antibodies-online.com)
  • Immunohistochemical staining experiments revealed DSiaT expression in ovaries, which implies a possible correlation between the sialyltransferase protein and oogenesis. (tamu.edu)
  • Biochemical studies indicate that the obtained protein is (1) an α2,3-sialyltransferase (main function), (2) an α2,6-sialyltransferase, (3) an α2,3-sialidase, and (4) an α2,3-trans-sialidase. (elsevier.com)
  • alpha2,3-sialyltransferase anyone. (bio.net)
  • Subject: alpha2,3-sialyltransferase anyone. (bio.net)
  • Identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, ST6Gal II. (nih.gov)
  • To elucidate the structural basis for the broad acceptor substrate specificity, we determined the crystal structure of the α2,3-sialyltransferase in complex with CMP. (elsevier.com)
  • Recently, several bacterial sialyltransferases were shown to exhibit broader substrate specificity than that of mammalian counterparts. (go.jp)
  • Our ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Peptides and ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Proteins can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Peptides and Proteins. (novusbio.com)
  • To test whether overexpression of Sia(α2,6)Gal moieties in cultured cells could make them suitable for testing human influenza virus sensitivity to NAI, we stably transfected MDCK cells with cDNA of human 2,6-sialyltransferase (SIAT1). (asm.org)
  • Statistically significant tissue- and type of stress-specific changes in total sialyltransferase (ST) activity were observed. (nih.gov)
  • Sialyltransferase activity and hepatic tumor growth in a nude mouse model of colorectal cancer metastases. (semanticscholar.org)
  • A highly sensitive fluorometric assay for sialyltransferase activity using CMP-9-fluoresceinyl-NeuAc as donor. (semanticscholar.org)
  • Increased CMP-NeuAc:Gal beta 1,4GlcNAc-R alpha 2,6 sialyltransferase activity in human colorectal cancer tissues. (semanticscholar.org)
  • Changes in sialyltransferase activity during murine T cell differentiation. (semanticscholar.org)
  • Occurrence of sialyltransferase activity in the synaptosomal membranes prepared from calf brain cortex. (semanticscholar.org)
  • Current trends in the structure-activity relationships of sialyltransferases. (semanticscholar.org)
  • Butyrate (5 mM) induces the sialyltransferase after a 4-h lag, producing maximum specific activity by 24 h. (rupress.org)
  • However, little is known about the presence and enzymatic activity of ganglioside sialyltransferases at the cell surface of macrophages, one of the most important immune cells involved in the innate inflammatory process. (elsevier.com)
  • Taken together, the data supports the presence and activity of sialyltransferases at the plasma membrane of RAW264.7 cells. (elsevier.com)
  • To determine which amino acid residues are essential for the catalytic activity of mouse Gal1,3GalNAc 2,3-sialyltransferase (mST3Gal I), chemical modification and site-directed mutagenesis were employed against tryptophan and cysteine residues located in the predicted catalytic domain. (who.int)
  • The multifunctionality is thus a common feature of many bacterial sialyltransferases. (pubmedcentralcanada.ca)
  • Kemmner W, Krück D, Schlag PM (1994) Different sialyltransferase activities in human colorectal carcinoma cells from surgical specimens detected by specific glycoprotein and glycolipid acceptors. (springer.com)
  • Here, we discuss the expression patterns of Siglecs and sialyltransferases (STs) in gynecologic cancers, including breast, ovarian, and uterine malignancies, based on evidence from The Cancer Genome Atlas. (frontiersin.org)
  • Thus DOC and TPA both downregulated, and did not upregulate, α-2,6-sialyltransferase expression in vitro, but by different transduction pathways. (elsevier.com)
  • Among the many significant changes in glycogene expression observed, of particular interest was the observation that an additional α2,6-sialyltransferase, ST6 (α-N-acetyl-neuraminyl-2,3-β-galactosyl-1,3)-N-acetylgalactosaminide α2,6-sialyltransferase 5 (ST6GalNAcV), was expressed at very lowlevels in all glioma and glioma cell lines examined compared with normal brain. (utmb.edu)
  • Sialyltransferases are categorized into 4 families on the basis of the carbohydrate side chain they synthesize, namely, ST3Gal ( α 2, 3-ST), ST6Gal ( α 2, 6-ST), ST6GalNAc, and ST8Sia ( α 2, 8-ST) [ 4 ]. (hindawi.com)
  • Immune regulation by the ST6Gal sialyltransferase. (semanticscholar.org)
  • Remodeling of marrow hematopoietic stem and progenitor cells by non-self ST6Gal-1 sialyltransferase. (sdsc.edu)
  • Cumulative evidence indicates which the sialyltransferase ST6Gal-1 as well as the sialyl-glycans which it constructs are functionally pleiotropic. (rectalcancersite.com)
  • Two kinds of cDNA encoding mouse Galβ1,3(4)GlcNAc α2,3-sialyltransferase (mST3Gal III) and Galβ1,4(3)GlcNAc α2,3-sialyltransferase (mST3Gal IV) were isolated from mouse brain cDNA library by means of a PCR-based approach. (kribb.re.kr)
  • The ST3Gal-I sialyltransferase controls CD8+ T lymphocyte homeostasis by modulating O-glycan biosynthesis. (semanticscholar.org)