Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
The major sialoglycoprotein of the human erythrocyte membrane. It consists of at least two sialoglycopeptides and is composed of 60% carbohydrate including sialic acid and 40% protein. It is involved in a number of different biological activities including the binding of MN blood groups, influenza viruses, kidney bean phytohemagglutinin, and wheat germ agglutinin.
A system of universal human blood group isoantigens with many associated subgroups. The M and N traits are codominant and the S and s traits are probably very closely linked alleles, including the U antigen. This system is most frequently used in paternity studies.
A family of SIALIC ACID binding proteins found in vertebrate species. They are transmembrane proteins which act as cell surface receptors for a variety of sialylated GLYCOCONJUGATES. While a subset of siglec protein subtypes are evolutionarily conserved between mammalian species, there are many others that are species specific.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
A plant genus of the family FABACEAE. It contains a hemagglutinin.
A strong oxidizing agent.
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
A class of inorganic or organic compounds that contain the borohydride (BH4-) anion.
Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.
A nucleoside monophosphate sugar which donates N-acetylneuraminic acid to the terminal sugar of a ganglioside or glycoprotein.
A lectin and cell adhesion molecule found in B-LYMPHOCYTES. It interacts with SIALIC ACIDS and mediates signaling from B-CELL ANTIGEN RECEPTORS.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
The sum of the weight of all the atoms in a molecule.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
A group of enzymes with the general formula CMP-N-acetylneuraminate:acceptor N-acetylneuraminyl transferase. They catalyze the transfer of N-acetylneuraminic acid from CMP-N-acetylneuraminic acid to an acceptor, which is usually the terminal sugar residue of an oligosaccharide, a glycoprotein, or a glycolipid. EC 2.4.99.-.
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The rate dynamics in chemical or physical systems.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Established cell cultures that have the potential to propagate indefinitely.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
Corneal and conjunctival dryness due to deficient tear production, predominantly in menopausal and post-menopausal women. Filamentary keratitis or erosion of the conjunctival and corneal epithelium may be caused by these disorders. Sensation of the presence of a foreign body in the eye and burning of the eyes may occur.
The fluid secreted by the lacrimal glands. This fluid moistens the CONJUNCTIVA and CORNEA.
The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball.
Dryness of the eye surfaces caused by deficiency of tears or conjunctival secretions. It may be associated with vitamin A deficiency, trauma, or any condition in which the eyelids do not close completely.
Drying and inflammation of the conjunctiva as a result of insufficient lacrimal secretion. When found in association with XEROSTOMIA and polyarthritis, it is called SJOGREN'S SYNDROME.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Chronic, non-specific inflammation of the GASTROINTESTINAL TRACT. Etiology may be genetic or environmental. This term includes CROHN DISEASE and ULCERATIVE COLITIS.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Inflammation of the COLON that is predominantly confined to the MUCOSA. Its major symptoms include DIARRHEA, rectal BLEEDING, the passage of MUCUS, and ABDOMINAL PAIN.
A chronic transmural inflammation that may involve any part of the DIGESTIVE TRACT from MOUTH to ANUS, mostly found in the ILEUM, the CECUM, and the COLON. In Crohn disease, the inflammation, extending through the intestinal wall from the MUCOSA to the serosa, is characteristically asymmetric and segmental. Epithelioid GRANULOMAS may be seen in some patients.
Cells that can carry out the process of PHAGOCYTOSIS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).
Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins found in any species of fungus.
A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.

Myocardial osteopontin expression coincides with the development of heart failure. (1/2476)

To identify genes that are differentially expressed during the transition from compensated hypertrophy to failure, myocardial mRNA from spontaneously hypertensive rats (SHR) with heart failure (SHR-F) was compared with that from age-matched SHR with compensated hypertrophy (SHR-NF) and normotensive Wistar-Kyoto rats (WKY) by differential display reverse transcriptase-polymerase chain reaction. Characterization of a transcript differentially expressed in SHR-F yielded a cDNA with homology to the extracellular matrix protein osteopontin. Northern analysis showed low levels of osteopontin mRNA in left ventricular myocardium from WKY and SHR-NF but a markedly increased (approximately 10-fold) level in SHR-F. In myocardium from WKY and SHR-NF, in situ hybridization showed only scant osteopontin mRNA, primarily in arteriolar cells. In SHR-F, in situ hybridization revealed abundant expression of osteopontin mRNA, primarily in nonmyocytes in the interstitial and perivascular space. Similar findings for osteopontin protein were observed in the midwall region of myocardium from the SHR-F group. Consistent with the findings in SHR, osteopontin mRNA was minimally increased (approximately 1.9-fold) in left ventricular myocardium from nonfailing aortic-banded rats with pressure-overload hypertrophy but was markedly increased (approximately 8-fold) in banded rats with failure. Treatment with captopril starting before or after the onset of failure in the SHR reduced the increase in left ventricular osteopontin mRNA levels. Thus, osteopontin expression is markedly increased in the heart coincident with the development of heart failure. The source of osteopontin in SHR-F is primarily nonmyocytes, and its induction is inhibited by an angiotensin-converting enzyme inhibitor, suggesting a role for angiotensin II. Given the known biological activities of osteopontin, including cell adhesion and regulation of inducible nitric oxide synthase gene expression, these data suggest that it could play a role in the pathophysiology of heart failure.  (+info)

An intramembrane modulator of the ErbB2 receptor tyrosine kinase that potentiates neuregulin signaling. (2/2476)

The ErbB2 receptor tyrosine kinase plays a critical role in a variety of developmental processes, and its aberrant activation may contribute to the progression of some breast and ovarian tumors. ASGP2, a transmembrane glycoprotein found on the surface of the highly metastatic ascites 13762 rat mammary adenocarcinoma cell line, is constitutively associated with ErbB2 in these cells and in mammary tissue from pregnant rats. Expression studies indicate that ASGP2 interacts directly and specifically with ErbB2 through one of its epidermal growth factor-like domains and that the co-expression of the two proteins in the same cell dramatically facilitates their direct stable interaction. Ectopic expression of ASGP2 in human melanoma tumor cells potentiates the response of endogenous ErbB2 to the neuregulin-1 growth factor. These observations point to a novel intramembrane mechanism for the modulation of receptor tyrosine kinase activity.  (+info)

Angiosarcomas express mixed endothelial phenotypes of blood and lymphatic capillaries: podoplanin as a specific marker for lymphatic endothelium. (3/2476)

Angiosarcomas apparently derive from blood vessel endothelial cells; however, occasionally their histological features suggest mixed origin from blood and lymphatic endothelia. In the absence of specific positive markers for lymphatic endothelia the precise distinction between these components has not been possible. Here we provide evidence by light and electron microscopic immunohistochemistry that podoplanin, a approximately 38-kd membrane glycoprotein of podocytes, is specifically expressed in the endothelium of lymphatic capillaries, but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with vascular endothelial growth factor receptor-3, the only other lymphatic marker presently available. Complementary immunostaining of blood vessels was obtained with established endothelial markers (CD31, CD34, factor VIII-related antigen, and Ulex europaeus I lectin) as well as podocalyxin, another podocytic protein that is also localized in endothelia of blood vessels. Podoplanin specifically immunolabeled endothelia of benign tumorous lesions of undisputed lymphatic origin (lymphangiomas, hygromas) and was detected there as a 38-kd protein by immunoblotting. As paradigms of malignant vascular tumors, poorly differentiated (G3) common angiosarcomas (n = 8), epitheloid angiosarcomas (n = 3), and intestinal Kaposi's sarcomas (n = 5) were examined for their podoplanin content in relation to conventional endothelial markers. The relative number of tumor cells expressing podoplanin was estimated and, although the number of cases in this preliminary study was limited to 16, an apparent spectrum of podoplanin expression emerged that can be divided into a low-expression group in which 0-10% of tumor cells contained podoplanin, a moderate-expression group with 30-60% and a high-expression group with 70-100%. Ten of eleven angiosarcomas and all Kaposi's sarcomas showed mixed expression of both lymphatic and blood vascular endothelial phenotypes. By double labeling, most podoplanin-positive tumor cells coexpressed endothelial markers of blood vessels, whereas few tumor cells were positive for individual markers only. From these results we conclude that (1) podoplanin is a selective marker of lymphatic endothelium; (2) G3 angiosarcomas display a quantitative spectrum of podoplanin-expressing tumor cells; (3) in most angiosarcomas, a varying subset of tumor cells coexpresses podoplanin and endothelial markers of blood vessels; and (4) all endothelial cells of Kaposi's sarcomas expressed the lymphatic marker podoplanin.  (+info)

Interleukin-1 receptor antagonist gene polymorphism and coronary artery disease. (4/2476)

BACKGROUND: Cytokine gene variations are contributory factors in inflammatory pathology. Allele frequencies of interleukin (IL)-1 cluster genes [IL-1A(-889), IL-1B(-511), IL-1B(+3953), IL-1RN Intron 2 VNTR] and tissue necrosis factor (TNF)-alpha gene [TNFA(-308)] were measured in healthy blood donors (healthy control subjects), patients with angiographically normal coronary arteries (patient control subjects), single-vessel coronary disease (SVD), and those with multivessel coronary disease (MVD). METHODS AND RESULTS: Five hundred fifty-six patients attending for coronary angiography in Sheffield were studied: 130 patient control subjects, 98 SVD, and 328 MVD. Significant associations were tested in an independent population (London) of 350: 57 SVD, 191 MVD, and 102 control subjects. IL-1RN*2 frequency in Sheffield patient control subjects was the same as in 827 healthy control subjects. IL-1RN*2 was significantly overrepresented in Sheffield SVD patients (34% vs 23% in patient control subjects); IL-1RN*2 homozygotes in the SVD population (chi2 carriage=8.490, 1 df, P=0.0036). This effect was present though not quite significant in the London population (P=0. 0603). A summary trend test of the IL-1RN SVD genotype data for Sheffield and London showed a significant association with *2 (P=0. 0024). No significant effect of genotype at IL-1RN was observed in the Sheffield or London MVD populations. Genotype distribution analysis comparing the SVD and MVD populations at IL-1RN showed a highly significant trend (P=0.0007) with the use of pooled data. No significant associations were seen for the other polymorphisms. CONCLUSIONS: IL-1RN*2 was significantly associated with SVD. A difference in genetic association between SVD and MVD was also apparent.  (+info)

A sialoglycoprotein, gp20, of the human capacitated sperm surface is a homologue of the leukocyte CD52 antigen: analysis of the effect of anti-CD52 monoclonal antibody (CAMPATH-1) on capacitated spermatozoa. (5/2476)

In this study we performed N-terminal sequence analysis of gp20, a 20 kDa sialoglycoprotein on the human sperm surface previously identified by radiolabelling of the sialic acid residues of sperm surface. We found 100% identity with the N-terminus of CD52, an antigen expressed on almost all human leukocytes. We also show that, like CD52, gp20 behaves as a glycosylphosphatidylinositol (GPI)-anchored protein and that anti-gp20 antiserum reacts with an antigen on leukocytes of the same molecular weight as CD52. Using CAMPATH-1, the monoclonal antibody against CD52, in fluorescent staining of capacitated spermatozoa, Western blot analysis and the zona-free hamster egg penetration test, we found that the effect of this antibody was different from that of our anti-gp20. Western blot analysis revealed a well-defined 20 kDa band with anti-gp20, whereas a 14-20 kDa band was detected with CAMPATH-1. Anti-gp20 stained the equatorial region of the sperm head, whereas CAMPATH-1 stained the tail in immunofluorescence analysis of capacitated spermatozoa. A dose-dependent inhibitory effect was seen with CAMPATH-1, similar to that previously detected with anti-gp20, in a zona-free hamster egg penetration test. However, with CAMPATH-1 agglutination of motile spermatozoa was detected, and this was not present with anti-gp20. This suggests that the epitopes recognized by the two antibodies are different.  (+info)

Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells. (6/2476)

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO+ memory subset and a CD45RA+ naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4. The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral CD4 T cells and 1D4+ CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1 D4 antigen is human CD43. Transfection of CHO-leu cells, which stably express human CD43, with core 2 beta-1,6-N-acetylglucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only in 1D4+ T cells but not in 1D4- T cells, implying that the 1 D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1 D4 was completely abolished when cells were treated with neuraminidase, while them remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans. Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT, whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305. Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO+ memory subset of CD4 T cells.  (+info)

Constitutive expression of interleukin-1alpha precursor promotes human vascular smooth muscle cell proliferation. (7/2476)

Vascular smooth muscle cell (VSMC) proliferation plays a critical role in the failure of vascular surgeries and contributes to the development of atherosclerotic lesions. Evidence that interleukin-1 (IL-1) is a mitogen for cultured VSMC has implicated its release by activated macrophages in the development of atherosclerosis. VSMC also produce IL-1, including the precursor form of IL-1alpha. However, it is not known whether IL-1alpha precursor is processed to mature IL-1alpha or released from VSMC, nor is it known whether either precursor or mature IL-1alpha functions as an autocrine growth factor. The goals of the present study were to establish whether proliferation is enhanced in human VSMC transfectants producing IL-1alpha constitutively at levels comparable to those produced after activation, and to determine which domains of IL-1alpha are important for its activity. Human VSMC were stably transfected with expression vectors directing constitutive expression of either full-length IL-1alpha precursor [IL-1alpha-(1-271)], its NH2-terminal domain [IL-1alpha-(1-112)], or mature IL-1alpha [IL-1alpha-(113-271)]. Both IL-1alpha-(1-271) and IL-1alpha-(113-271) stable transfectants produced moderate levels of IL-1alpha (0.2-1.0 ng/10(6) cells) and released low levels of IL-1alpha into the supernatant (<20 pg/ml). VSMC stably transfected with either IL-1alpha-(1-271) or IL-1alpha-(113-271) expression plasmids proliferated rapidly compared with nontransfected or vector-transfected VSMC and displayed a distinct morphology characterized by elongated, spindle-shaped cells. Stable transfection with IL-1alpha-(1-271) was somewhat more effective than transfection with IL-1alpha-(113-271). Interestingly, VSMC transfected with IL-1alpha-(113-271) expression plasmids also expressed IL-1alpha-(1-271) mRNA, suggesting that IL-1alpha-(113-271) activates an IL-1-induced IL-1 autocrine loop. In contrast, neither proliferation rates nor morphology was affected by stable transfection with IL-1alpha-(1-112) expression plasmids. Exogenous IL-1 receptor antagonist partially reversed the enhanced DNA synthesis in VSMC transfected with either IL-1alpha-(1-271) or IL-1alpha-(113-271) expression plasmids, suggesting that the pro-proliferative effect of VSMC-derived IL-1alpha is at least partially mediated by signaling via the type I IL-1 receptor. These results demonstrate that IL-1alpha precursor is an autocrine growth factor for human VSMC and further indicate that amino acids 113-271 play a crucial role in its actions.  (+info)

Acute-phase responses in transgenic mice with CNS overexpression of IL-1 receptor antagonist. (8/2476)

The interleukin-1 (IL-1) receptor antagonist (IL-1ra) is an endogenous antagonist that blocks the effects of the proinflammatory cytokines IL-1alpha and IL-1beta by occupying the type I IL-1 receptor. Here we describe transgenic mice with astrocyte-directed overexpression of the human secreted IL-1ra (hsIL-1ra) under the control of the murine glial fibrillary acidic protein (GFAP) promoter. Two GFAP-hsIL-1ra strains have been generated and characterized further: GILRA2 and GILRA4. These strains show a brain-specific expression of the hsIL-1ra at the mRNA and protein levels. The hsIL-1ra protein was approximated to approximately 50 ng/brain in cytosolic fractions of whole brain homogenates, with no differences between male and female mice or between the two strains. Furthermore, the protein is secreted, inasmuch as the concentration of hsIL-1ra in the cerebrospinal fluid was 13 (GILRA2) to 28 (GILRA4) times higher in the transgenic mice than in the control animals. To characterize the transgenic phenotype, GILRA mice and nontransgenic controls were injected with recombinant human IL-1beta (central injection) or lipopolysaccharide (LPS, peripheral injection). The febrile response elicited by IL-1beta (50 ng/mouse icv) was abolished in hsIL-1ra-overexpressing animals, suggesting that the central IL-1 receptors were occupied by antagonist. The peripheral LPS injection (25 micrograms/kg ip) triggered a fever in overexpressing and control animals. Moreover, no differences were found in LPS-induced (100 and 1,000 micrograms/kg ip; 1 and 6 h after injection) IL-1beta and IL-6 serum levels between GILRA and wild-type mice. On the basis of these results, we suggest that binding of central IL-1 to central IL-1 receptors is not important in LPS-induced fever or LPS-induced IL-1beta and IL-6 plasma levels.  (+info)

TY - JOUR. T1 - Podocalyxin-like protein 1 regulates TAZ signaling and stemness properties in colon cancer. AU - Lee, Wen Ying. AU - Kuo, Chih Chia. AU - Lin, Bo Xing. AU - Cheng, Chia Hsiung. AU - Chen, Ku Chung. AU - Lin, Cheng Wei. PY - 2017/10/1. Y1 - 2017/10/1. N2 - Colon cancer is the third most common cancer in the world and the second most common cause of cancer-related mortality. Molecular biomarkers for colon cancer have undergone vigorous discovery and validation. Recent studies reported that overexpression of podocalyxin-like protein 1 (PODXL) is associated with distant metastasis and poor prognosis across several types of malignancies. Its role and underlying molecular mechanism, however, are not yet fully understood. In the present study, we revealed that the Hippo transducer, the transcriptional coactivator with PDZ-binding motif (TAZ), acts as a downstream mediator of PODXL in colon cancer. Inhibition of PODXL resulted in the suppression of TAZ signaling and the downregulation of ...
BACKGROUND: Podocalyxin-like 1 (PODXL) is a cell-adhesion glycoprotein and stem cell marker that has been associated with an aggressive tumour phenotype and poor prognosis in several forms of cancer. In this study, we investigated the prognostic impact of PODXL expression in colorectal cancer (CRC). METHODS: Using tissue microarrays and immunohistochemistry, PODXL expression was evaluated in 536 incident CRC cases from a prospective, population-based cohort study. Kaplan-Meier analysis and Cox proportional hazards modelling were used to assess the impact of PODXL expression on cancer-specific survival (CSS) and overall survival (OS). RESULTS: High PODXL expression was significantly associated with unfavourable clinicopathological characteristics, a shorter CSS (hazard ratio (HR) = 1.98; 95% confidence interval (CI) 1.38-2.84, P , 0.001) and 5-year OS (HR = 1.85; 95% CI 1.29-2.64, P = 0.001); the latter remaining significant in multivariate analysis (HR = 1.52; 95% CI 1.03-2.25, P = 0.036). In ...
The PODXL gene encodes for the podocalyxin-like protein 1 which is expressed in podocytes of the kidney glomeruli. PODXL is involved in the regulation of cell adhesion and cell morphology and functions to maintain the filtration pathway in glomeruli by anti-adhesive action on podocytes. PODXL has also been implicated in cancer progression by inducing cell migration and invasion. Based on its role in cancer, PODXL has been suggested as an emerging prognostic biomarker in various forms of cancer such as colorectal cancer ...
Purpose: : PODXL2 (also known as Endoglycan) is a protein closely related to CD34 and podocalyxin. Recent studies have implicated PODXL2 in leukocyte functions, while our own protein association assays have indicated potential interactions with retinal proteins involved in the development of Usher Syndrome. Little is currently known about this proteins role in healthy retinal function. This study seeks to determine the tissue expression pattern of PODXL2 and eludicate its role in retinal function and maintenance using genetic mouse models. Methods: : We developed mutant lines of mice with VelociGenes predesigned construct (#10133) from the KOMP. Expression of PODXL2 mRNA and protein was evaluated by RT-PCR, Immunoblotting, Immunofluorescence, and by following a lacZ reporter gene embedded in the gene targeting construct. Retinal function in the PODXl2 mice was assessed by ERG recordings. Results: : The knockout construct integration has been confirmed in PODXL2 -/- mice, with several regions ...
Definition of sialoglycoproteins in the Definitions.net dictionary. Meaning of sialoglycoproteins. What does sialoglycoproteins mean? Information and translations of sialoglycoproteins in the most comprehensive dictionary definitions resource on the web.
Reactivity: Chicken, Cow, Dog and more. Compare 47 different PODXL ELISA Kits & buy the right one directly at antibodies-online.com!
Background: Podocalyxin-like 1 (PODXL) is an anti-adhesive transmembrane protein that has been demonstrated to be an independent factor of poor prognosis in colorectal cancer (CRC). The gene encoding PODXL is located to chromosome 7, which also harbours the gene for the epidermal growth factor receptor (EGFR). The aim of this study was to examine the associations between PODXL and EGFR expression in CRC in vitro and in vivo.. Methods: EGFR expression was analysed in tumours from three independent patient cohorts; cohort 1 (n = 533), cohort 2 (n = 259) and cohort 3 (n = 310), previously analysed for immunohistochemical PODXL expression and KRAS and BRAF mutations (cohort 1 and 3). Levels of EGFR and PODXL were determined by western blot in six different CRC cell lines.. Results: High expression of PODXL was significantly associated with high EGFR expression (p , 0.001) in all three cohorts, and with BRAF mutation (p , 0.001) in cohort 1 and 3. High EGFR expression correlated with BRAF mutation (p ...
Complete information for PODXL gene (Protein Coding), Podocalyxin Like, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
CHO-Anti-Human PODXL2 F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human PODXL2 F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Podocalyxin (gene name PODXL) is a CD34-related sialomucin implicated in the regulation of cell adhesion, migration and polarity. Upregulated expression of podocalyxin is linked to poor patient survival in epithelial cancers. However, it is not known if podocalyxin has a functional role in tumor progression. We silenced podocalyxin expression in the aggressive basal-like human (MDA-MB-231) and mouse (4T1) breast cancer cell lines and also overexpressed podocalyxin in the more benign human breast cancer cell line, MCF7. We evaluated how podocalyxin affects tumorsphere formation in vitro and compared the ability of podocalyxin-deficient and podocalyxin-replete cell lines to form tumors and metastasize using xenogenic or syngeneic transplant models in mice. Finally, in an effort to develop therapeutic treatments for systemic cancers, we generated a series of antihuman podocalyxin antibodies and screened these for their ability to inhibit tumor progression in xenografted mice. Although deletion of
SwePub titelinformation: Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer
Sialoglycoproteins: Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
PODXL兔多克隆抗体(ab104988)可与人样本反应并经WB, IHC, ICC/IF实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
In this study, we investigated the use of metabolic oligosaccharide engineering and bio-orthogonal ligation reactions combined with lectin microarray and mass spectrometry to analyze sialoglycoproteins in the SW1990 human pancreatic cancer line. Specifically, cells were treated with the azido N-acetylmannosamine analog, 1,3,4-Bu3ManNAz, to label sialoglycoproteins with azide-modified sialic acids. The metabolically labeled sialoglyproteins were then biotinylated via the Staudinger ligation, and sialoglycopeptides containing azido-sialic acid glycans were immobilized to a solid support. The peptides linked to metabolically labeled sialylated glycans were then released from sialoglycopeptides and analyzed by mass spectrometry; in parallel, the glycans from azido-sialoglycoproteins were characterized by lectin microarrays. This method identified 75 unique N-glycosite-containing peptides from 55 different metabolically labeled sialoglycoproteins of which 42 were previously linked to cancer in the literature
The authors describe a highly organized membrane-bound lumenal compartment that they name the apicosome. They find that the apicosome is an intracellular perinuclear entity rich in apical proteins, positively expressing F-actin, EZRIN, and PODOCALYXIN (PODXL), among others. High accumulation of the marker PODXL has been identified in apicosomal-like structures of mouse blastocysts (Bedzhov et al., 2014). Taniguchi et al. (2017) also show that the apicosome is not present in mouse embryonic stem cells, which makes it a unique property of single-plated hPSCs and mouse epiblast cells. Its novelty is also highlighted by the fact that it does not costain with other organelle markers, making it an independent structure.. By the combination of ultrastructural analyses and live-cell imaging of isolated hPSCs expressing an EZRIN-GFP fusion protein, Taniguchi et al. (2017) characterize the apicosome as a membrane-bound lumen with a diameter of 3-5 µm. It is demarcated by a membrane containing highly ...
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PubMedID: 25396425 | Adhesive and migratory effects of phosphophoryn are modulated by flanking peptides of the integrin binding motif. | PloS one | 11/15/2014
ニワトリ・ポリクローナル抗体 ab26910 交差種: Hu 適用: WB…PODXL抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
Definition: D2-40 is a monoclonal antibody recognizing podoplanin , a 40 000 kD O-linked sialoglycoprotein located in lymphatic endothelial (...)
In a previous study, we demonstrated that nucleotides induce an increase in OPN mRNA steady-state levels in SMCs cultured in the presence or absence of serum.6 The present work shows that the UTP-induced OPN mRNA increase in rat SMCs results from both OPN mRNA stabilization and OPN gene transcription.. To date, few studies have tried to establish the mechanisms of OPN mRNA increase. In particular, it has been shown that neither transforming growth factor-β, a growth factor activating serine/threonine kinase receptors, nor intracellular receptor agonists, such as glucocorticoids and retinoic acid,37,38 induce OPN mRNA stability.3 In this study, we demonstrate that OPN mRNA level is regulated by UTP via a mRNA stabilization mechanism. Ang II, another agonist of G-protein-coupled receptors, can also stabilize OPN mRNA but not PDGF (data not shown). The sequence analysis of OPN mRNA reveals one potential sequence involved in mRNA stability in the 3′ UTR, TAAAT (located at 1084 to 1088). However, ...
R&D Systems™ Bovine Osteopontin/OPN Protein 50ug R&D Systems™ Bovine Osteopontin/OPN Protein Proteins O
Several studies indicate that local immunoregulation and associated cytokines have a putative role in the development of cancer. There is evidence that pro-inflammatory cytokines such as interleukin-1 (IL-1) are critically involved with tumour progression. IL-1 receptor antagonist (IL-1Ra) is known to down regulate and limit the inflammatory response. Therefore we attempted to examine the influence of the known polymorphism of the IL-1Ra gene on the development of human colorectal cancer (CRC). The study included 125 patients with CRC and 134 controls. Variable number tandem repeat (VNTR) polymorphism in intron 2 of the IL-Ra gene was analysed by the polymerase chain reaction method. There was a significant difference in genotype distribution between CRC patients and controls (P=0.025) and also in allelic frequencies (P=0.012). In detail the carriage rate of allele 3 in CRC patients was significantly increased compared with controls (P=0.007). We also found that the allelic distribution differs ...
TY - JOUR. T1 - Prognostic significance of osteopontin expression in human prostate cancer. AU - Forootan, Shiva S. AU - Foster, Christopher S. AU - Aachi, Vijay R. AU - Adamson, Janet. AU - Smith, Paul H. AU - Lin, Ke. AU - Ke, Youqiang. N1 - 2005 Wiley-Liss, Inc.. PY - 2006/5/1. Y1 - 2006/5/1. N2 - To test the hypothesis that expression of osteopontin (OPN), an integrin-binding glycoprotein, can independently predict the potential aggressiveness of prostate cancer, the status of OPN expression in benign and malignant prostate cancer cell lines and tissues was analysed by Western blot and immunohistochemistry. Amongst the four prostate cell lines analysed, the level of OPN expressed in the benign PNT-2 cells was set at 1, the relative level of OPN expressed in the weakly malignant cell line LNCaP was increased to 1.5. In the highly malignant cell lines Du-145 and PC-3, the level of OPN expression was further increased to 2.9 and 4.4, respectively. An increased expression of OPN was also ...
Leukosialin also known as sialophorin or CD43 (cluster of differentiation 43) is a transmembrane cell surface protein that in humans is encoded by the SPN (sialophorin) gene. Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. It also appears in about 25% of intestinal MALTomas.[citation needed] Using immunohistochemistry, CD43 can be demonstrated in the paracortical T-cells of healthy lymph nodes and tonsils; it is also positive in a range of lymphoid and myeloid tumours. Although it is present in over 90% of T-cell lymphomas, it is generally less effective at demonstrating this condition than is CD3 antigen. However, it may be useful as part of a panel to demonstrate B-cell ...
Osteopontin (OPN) is abundantly expressed in human calcified arteries. To examine the role of OPN in vascular calcification, OPN mutant mice were crossed with m
Sharp, Julie A., Sung, V., Slavin, J., Thompson, E. W. and Henderson, M. A. 1999, Tumor cells are the source of osteopontin and bone sialoprotein expression in human breast cancer, Laboratory investigation, vol. 79, no. 7, pp. 869-877. ...
Expression of PODXL (Gp200, PC, PCLP) in liver tissue. Antibody staining with HPA002110, HPA045507, CAB016169, CAB062558, CAB068219 and CAB068220 in immunohistochemistry.
Osteopontin has long been implicated in the process of carcinogenesis, progression, and metastatic dissemination of several human tumors, such as breast (12, 13), prostate (15), colon (16), ovarian (17), gastric (18), and lung (14) cancers and more recently in many other tumors, such as pancreatic, renal, endometrial, esophageal, and head and neck carcinomas (30).. In the present study, we decided to focus on osteopontin protein expression detected by immunohistochemistry in a large sample (207 cases) of NSCLCs, because these tumors still have a poor prognosis in spite of the notable advances in diagnosis, staging, treatment, and biological characterization. We analyzed the correlations between osteopontin expression and many clinicopathologic variables to clarify its possible prognostic role. As far as we know, our study is the largest retrospective analysis of the prognostic role of osteopontin expression in patients with NSCLC treated with curative surgery. Moreover, we decided to give ...
Quantikine® ELISA kit for human Osteopontin/OPN (Cat#SOST00). 0.024 ng/mL detection sensitivity. View Osteopontin/OPN ELISA kit details.
The design and selection criteria of the Framingham Offspring study have been described previously.2 On the basis of the sex-specific distributions of echocardiographic LV measurements, we sampled participants from the sixth examination cycle (1995-8) with both LV end diastolic dimension (LVEDD) and wall thickness (LVWT) below the sex-specific median (referent, n = 129), with increased LVEDD (⩾ 90th sex-specific centile, n = 134) and increased LVWT (⩾ 90th sex-specific centile, n = 128) in a 1:1:1 ratio. Eighteen participants were included in both LV dilatation and increased LVWT groups, so the study sample consisted of 373 participants. Participants underwent a standardised medical history and physical examination. Fasting blood samples were drawn and frozen at −70°C without any freeze-thaw cycles until assay. Plasma osteopontin was measured in duplicate by using an enzyme-linked immunosorbent assay (Calbiochem, Inc) with an intra-assay coefficient of variation of 4.6 ...
Chronic degenerative disease of the mares endometrium is characterised by changes in the uterine glands, including cystic dilation, hyperplasia and periglandular fibrosis. Endometrial biopsies were taken from 23 mares with different grades of endometrial degeneration. Solid structures were identified within the lumina of the uterine glands and shown to be calcified by histochemical staining. Most of them were not homogenous but composed of a mixture of mineral and organic substances. Further examinations of these mineralised structures by immunohistochemical methods revealed the presence of the non-collagenous matrix proteins osteopontin, osteonectin and bone sialoprotein, which are known to be involved in calcification processes such as urolithiosis. Osteopontin and bone sialoprotein were identified within the calculi, frequently arranged in concentric layers. Osteonectin was the only matrix protein that was also present in the glandular epithelium. Osteocalcin was not found in either the ...
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OPN (osteopontin), a pro-inflammatory cytokine, has recently emerged as a key factor in both vascular remodelling and the development of atherosclerosis. However, the relationship between OPN and atherosclerosis in patients without symptomatic cardiovascular disease is not clear. Therefore we measured plasma OPN levels and evaluated the correlation between plasma OPN levels and atherosclerosis as target organ damage in patients with EHT (essential hypertension). Plasma OPN levels were measured in 76 patients with EHT using a solid-phase sandwich ELISA. IMT (intima-media thickness), and Vd and Vs (mean diastolic and systolic flow velocities respectively) were evaluated by carotid ultrasound. The Vd/Vs ratio, an index of peripheral arterial resistance, was also calculated. The patients were divided on the basis of median OPN levels into a high-OPN group and a low-OPN group. The mean IMT and aldosterone levels were higher (P=0.024 and 0.031 respectively) and Vd/Vs was lower (P=0.007) in the ...
Recombinant Mouse CD25/IL2RA Protein (Met1-Lys236) 50292-M08H with a fusion His Tag, is expressed in HEK293 Cells. With high purity, high biological activity, high stability, and other superior features, you can use this Mouse CD25/IL2RA protein for relevant bioassay and related research.
These data demonstrate icIL-1ra type I mRNA, sIL-1ra mRNA, and IL-1ra protein in human coronary arteries. IL-1ra protein colocalizes with IL-1β predominantly in the endothelium of these arteries, with less IL-1ra mRNA expression occurring in less-diseased DCM arteries. To our knowledge, this is the first documentation of IL-1ra expression by HCAECs. We also show only icIL-1ra type I mRNA expression in HUVECs and HCAECs stimulated in vitro with LPS/PMA or TGF-β. Previous reports have failed to detect IL-1ra mRNA in these cell types with the use of other stimuli and less sensitive detection methods, such as Northern blots,5 and there are no previous reports of immunohistochemistry for IL-1ra in human vessels. With the use of immunoprecipitation/Western blot techniques, our data also confirm that the expression of IL-1ra in HUVECs and HCAECs is intracellular. We have also demonstrated that the rare allele (IL-1RN *2/*2) of a VNTR polymorphism in the IL-1RN gene is associated with significantly ...
Recombinant Human IL-5RA protein is a HEK 293 Protein fragment 21 to 335 aa range, | 85% purity, | 1.000 Eu/µg endotoxin level and validated in SDS-PAGE.
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Mouse Monoclonal Anti-CD43/Sialophorin Antibody (W3/13) [DyLight 488]. Validated: Flow, IHC-Fr, IHC-P. Tested Reactivity: Rat. 100% Guaranteed.
article{9ffbdf28-43ac-4664-bbde-373cd7e125c5, abstract = {The use of cancer biomarkers to anticipate the outlines of disease has been an emerging issue, especially as cancer treatment has made such positive steps in the last few years. Progress in the development of consistent malignancy markers is imminent because advances in genomics and bioinformatics have allowed the examination of immense amounts of data. Osteopontin is a phosphorylated glycoprotein secreted by activated macrophages, leukocytes, and activated T lymphocytes, and is present in extracellular fluids, at sites of inflammation, and in the extracellular matrix of mineralized tissues. Several physiologic roles have been attributed to osteopontin, i.e., in inflammation and immune function, in mineralized tissues, in vascular tissue, and in kidney. Osteopontin interacts with a variety of cell surface receptors, including several integrins and CD44. Binding of osteopontin to these cell surface receptors stimulates cell adhesion, ...
Buy our Recombinant Human IL5RA protein (Fc Chimera). Ab83828 is a protein fragment produced in HEK 293 cells and has been validated in SDS-PAGE. Abcam…
Promab CD106 - This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different
Promab CD106 - This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different
XCL1 / Lymphotactin Protein LS-G5664 is a Recombinant Mouse XCL1 / Lymphotactin aa 22-114 produced in E. coli. It is biologically active.
Bone Sialoprotein antibody (integrin-binding sialoprotein) for ELISA, IHC-P, RIA, WB. Anti-Bone Sialoprotein pAb (GTX44333) is tested in Human samples. 100% Ab-Assurance.
Rabbit anti Human Lymphotactin antibody recognizes human Lymphotactin, otherwise known as XCL1, the only member of the C-chemokine family
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IRAP (Interleukin-1 Receptor Antagonist Protein) is an anti-inflammation therapy that is best for patients suffering from Osteoarthritis.
Laminins [(PUBMED:2404817)] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. They are composed of distinct but related alpha, beta and gamma chains. The three chains form a cross-shaped molecule that consist of a long arm and three short globular arms. The long arm consist of a coiled coil structure contributed by all three chains and cross-linked by interchain disulphide bonds. Beside different types of globular domains each subunit contains, in its first half, consecutive repeats of about 60 amino acids in length that include eight conserved cysteines [(PUBMED:2666164)]. The tertiary structure [(PUBMED:8648630), (PUBMED:8648631)] of this domain is remotely similar in its N-terminal to that of the EGF-like module. It is known as a LE or laminin-type EGF-like domain. The number of copies of the LE domain in the different forms of laminins is highly variable; from 3 up to 22 copies have been found. A schematic ...
Laminins [(PUBMED:2404817)] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. They are composed of distinct but related alpha, beta and gamma chains. The three chains form a cross-shaped molecule that consist of a long arm and three short globular arms. The long arm consist of a coiled coil structure contributed by all three chains and cross-linked by interchain disulphide bonds. Beside different types of globular domains each subunit contains, in its first half, consecutive repeats of about 60 amino acids in length that include eight conserved cysteines [(PUBMED:2666164)]. The tertiary structure [(PUBMED:8648630), (PUBMED:8648631)] of this domain is remotely similar in its N-terminal to that of the EGF-like module. It is known as a LE or laminin-type EGF-like domain. The number of copies of the LE domain in the different forms of laminins is highly variable; from 3 up to 22 copies have been found. A schematic ...
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XCL1 protein is expressed in E. coli, processed, refolded and purified to yield the native, secreted form of the mature chemokine. XCL1 is a ligand for the G protein coupled receptor XCR1 with and EC50 ~ 50 nM. It has been shown the WT XCL1 is able to int
OPN3_MOUSE (Q9WUK7 ), OPN4A_DANRE (Q2KNE5 ), OPN4A_GADMO (Q804X9 ), OPN4B_GADMO (Q804Q2 ), OPN4B_XENLA (O57422 ), OPN4L_DANRE (Q1JPS6 ), OPN4_FELCA (Q5YKK9 ), OPN4_HUMAN (Q9UHM6 ), OPN4_MOUSE (Q9QXZ9 ), OPN4_PHOSU (Q5XXP2 ), OPN4_PODSI (Q4U4D2 ), OPN4_RAT (Q8R456 ), OPN4_RUTRU (Q6XL69 ), OPN5_HUMAN (Q6U736 ), OPN5_MOUSE (Q6VZZ7 ), OPS1_CALVI (P22269 ), OPS1_DROME (P06002 ), OPS1_DROPS (P28678 ), OPS1_MANSE (O02464 ), OPS1_SCHGR (Q94741 ), OPS2_DROME (P08099 ), OPS2_DROPS (P28679 ), OPS2_SCHGR (Q26495 ), OPS3_DROME (P04950 ), OPS3_DROPS (P28680 ), OPS4_DROME (P08255 ), OPS4_DROPS (P29404 ), OPS4_DROVI (P17646 ), OPS5_DROME (P91657 ), OPS6_DROME (O01668 ), OPSB_ANOCA (P51471 ), OPSB_APIME (P90680 ), OPSB_ASTFA (P51472 ), OPSB_BOVIN (P51490 ), OPSB_CARAU (P32310 ), OPSB_CHICK (P28682 ), OPSB_CONCO (O13227 ), OPSB_GEKGE (P35357 ), OPSB_HUMAN (P03999 ), OPSB_MOUSE (P51491 ), OPSB_ORYLA (P87365 ), OPSB_PANPA (P60573 ), OPSB_PANTR (P60015 ), OPSB_RAT (Q63652 ), OPSB_SAIBB (O13092 ), OPSC1_HEMSA (Q25157 ...
Major sialoglycoproteins carrying polylactosaminoglycan". The Journal of Biological Chemistry. 263 (35): 18911-9. PMID 3143719 ...
Tate CG, Tanner MJ (1988). "Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta". ... Facer CA (1983). "Erythrocyte sialoglycoproteins and Plasmodium falciparum invasion". Trans. R. Soc. Trop. Med. Hyg. 77 (4): ... are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood ... "Structures of Miltenberger class I and II specific major human erythrocyte membrane sialoglycoproteins". Eur. J. Biochem. 138 ( ...
"Binding of Transmissible Gastroenteritis Coronavirus to Cell Surface Sialoglycoproteins". Journal of Virology. 76 (12): 6037-43 ... "Binding of Transmissible Gastroenteritis Coronavirus to Brush Border Membrane Sialoglycoproteins". Journal of Virology. 77 (21 ...
In the erythrocyte glycophorin C makes up ~4% of the membrane sialoglycoproteins. The average number of O linked chains is 12 ... Glycophorin C and D are minor sialoglycoproteins contributing to 4% and 1% to the PAS-positive material and are present at ... Furthmayr H (1978). "Glycophorins A, B, and C: a family of sialoglycoproteins. Isolation and preliminary characterization of ...
Sutherland DR, Abdullah KM, Cyopick P, Mellors A (March 1992). "Cleavage of the cell-surface O-sialoglycoproteins CD34, CD43, ... This enzyme catalyses the following chemical reaction Hydrolysis of O-sialoglycoproteins; cleaves -Arg31-Asp- bond in ... "A neutral glycoprotease of Pasteurella haemolytica A1 specifically cleaves O-sialoglycoproteins". Infection and Immunity. 60 (1 ...
Tate CG, Tanner MJ (1988). "Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta". ... are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood ... "Structures of Miltenberger class I and II specific major human erythrocyte membrane sialoglycoproteins". Eur. J. Biochem. 138 ( ... healthy Japanese individuals of type MkMk have erythrocytes which lack both the blood group MN and Ss-active sialoglycoproteins ...
... containing glycoproteins (sialoglycoproteins) bind selectin in humans and other organisms. Metastatic cancer cells ...
2 as membrane-bound O-sialoglycoproteins with anti-adhesive activity". FEBS Letters. 499 (1-2): 121-6. doi:10.1016/S0014-5793( ...
2001). "Identification of human endomucin-1 and -2 as membrane-bound O-sialoglycoproteins with anti-adhesive activity". FEBS ...
CS1 maint: discouraged parameter (link) Sialoglycoproteins at the US National Library of Medicine Medical Subject Headings ( ...
Its adhesion proteins attach to tracheal epithelial cells by sialoglycoproteins or sialoglycolipid receptors, which are located ...
Sutherland, D.R., Abdullah, K.M., Cyopick, P. and Mellors, A. Cleavage of the cell-surface O-sialoglycoproteins CD34, CD 43, CD ... A neutral glycoprotease of Pasteurella haemolytica A1 specifically cleaves O-sialoglycoproteins". Infect. Immun. 60: 56-62. ...
... which in turn could affect sialoglycoproteins. GNE knockout mice show problems similar to people with IBM and in people with ...
... sialoglycoproteins MeSH D12.644.233.800.174 - antigens, cd43 MeSH D12.644.233.800.350 - glycophorin MeSH D12.644.233.900 - ...
Psathyrella velutina Mushroom Lectin Exhibits High Affinity toward Sialoglycoproteins Possessing Terminal N-Acetylneuraminic ...
Lin F., Wise G.E. (2002) Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using EosinY Stain. In: Walker J.M ... Mueller, T. J., Dow, A. W., and Morrison, M. (1976) Heterogeneity of the sialoglycoproteins of the normal human erythrocyte ... sialoglycoproteins cannot be detected by CBB and thus have to be visualized by other stains, such as the periodic acid-Schiff ( ... sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique. Proc. Natl. Acad. Sci. USA 81 ...
Sialoglycoproteins: Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the ... Sialoglycoproteins. Subscribe to New Research on Sialoglycoproteins Glycoproteins which contain sialic acid as one of their ... 08/01/2008 - "Various MAL-positive sialoglycoproteins were detected in cancer tissues but not in non-cancer tissues. ". 08/15/ ... 11/01/2005 - "In this study, we report that BCP-leukemia cells express selectin ligand on O-sialoglycoproteins. ". 01/01/1986 ...
Meaning of sialoglycoproteins. What does sialoglycoproteins mean? Information and translations of sialoglycoproteins in the ... Definition of sialoglycoproteins in the Definitions.net dictionary. ... What does sialoglycoproteins mean?. Definitions for sialoglycoproteins. Here are all the possible meanings and translations of ... Alternative searches for sialoglycoproteins:. *Search for Synonyms for sialoglycoproteins. *Search for Anagrams for ...
Background The Trypanosoma cruzi infection is associated with severe T cell unresponsiveness to antigens and mitogens characterized by decreased IL-2 synthesis. Trypanosoma cruzi mucin (Tc Muc) has been implicated in this phenomenom. These molecules contain a unique type of glycosylation consisting of several sialylated O-glycans linked to the protein backbone via N-acetylglucosamine residues. Methodology/Principal Findings In this study, we evaluated the ability of Tc Muc to modulate the activation of CD4+ T cells. Our data show that cross-linking of CD3 on naïve CD4+ T cells in the presence of Tc Muc resulted in the inhibition of both cytokine secretion and proliferation. We further show that the sialylated O-Linked Glycan residues from tc mucin potentiate the suppression of T cell response by inducing G1-phase cell cycle arrest associated with upregulation of mitogen inhibitor p27kip1. These inhibitory effects cannot be reversed by the addition of exogenous IL-2, rendering CD4+ T cells anergic when
RA Sack, S Sathe, A Beaton, B Bogart, OU Khan, P Asbell; Characterization of Proteins and Sialoglycoproteins in Normal and Dry ... Characterization of Proteins and Sialoglycoproteins in Normal and Dry Eye Tear Fluid ... Characterization of Proteins and Sialoglycoproteins in Normal and Dry Eye Tear Fluid ...
A neutral glycoprotease of Pasteurella haemolytica A1 specifically cleaves O-sialoglycoproteins.. @article{Abdullah1992ANG, ... title={A neutral glycoprotease of Pasteurella haemolytica A1 specifically cleaves O-sialoglycoproteins.}, author={Kamaruddin ...
... failed to increase either sialyltransferase activity or cell surface labeling of sialoglycoproteins. These findings suggest ... enhances the glycosylation of specific cell surface sialoglycoproteins, and stimulates sialytransferase activity. Mutant clones ... Correlation of retinoic acid-enhanced sialyltransferase activity and glycosylation of specific cell surface sialoglycoproteins ... Correlation of retinoic acid-enhanced sialyltransferase activity and glycosylation of specific cell surface sialoglycoproteins ...
Sialoglycoproteins in Human Serum. We further applied IntactGIG method for analysis of human serum sialoglycopeptides. Many ...
Targeted identification of metastasis-associated cell-surface sialoglycoproteins in prostate cancer.. Citation data: Molecular ... A selective enrichment of sialoglycoproteins was confirmed. When compared with global proteomic analysis of the same cells, the ... Our approach effectively targeted surface sialoglycoproteins and efficiently identified proteins that underlie the metastatic ... labeling with mass spectrometry analysis to identify and characterize metastasis-associated cell surface sialoglycoproteins. ...
Sialoglycoproteins / metabolism * Tumor Necrosis Factor-alpha / genetics * Tumor Necrosis Factor-alpha / metabolism ...
Sialoglycoproteins / genetics* Substances * IL1RN protein, human * Interleukin 1 Receptor Antagonist Protein * Interleukin-1 ...
Over expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac2-GPs, abbreviated as OAcSGP) has been demonstrated as a disease- ... Over expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac2-GPs, abbreviated as OAcSGP) has been demonstrated as a disease- ... Flow-cytometric monitoring of disease-associated expression of 9-O-acetylated sialoglycoproteins in combination with known CD ... O-acetylated sialoglycoproteins henceforth abbreviated as OAcSGP. Sialic acid binding lectins and/or monoclonal antibodies (mAb ...
Major sialoglycoproteins carrying polylactosaminoglycan". The Journal of Biological Chemistry. 263 (35): 18911-9. PMID 3143719 ...
Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins ...
0/Heat-Shock Proteins; 0/Sialoglycoproteins; 106441-73-0/Osteopontin; 9007-34-5/Collagen ...
Sialoglycoproteins); 0 (UNC5H4 protein, human); 0 (hook1 protein, human); 9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle ...
0/Sialoglycoproteins; 0/cagA protein, Helicobacter pylori ...
Tate CG, Tanner MJ (1988). "Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta". ... Facer CA (1983). "Erythrocyte sialoglycoproteins and Plasmodium falciparum invasion". Trans. R. Soc. Trop. Med. Hyg. 77 (4): ... are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood ... "Structures of Miltenberger class I and II specific major human erythrocyte membrane sialoglycoproteins". Eur. J. Biochem. 138 ( ...
Chemicals/CAS: interferon beta serine, 90598-63-3; osteopontin, 106441-73-0; Osteopontin, 106441-73-0; Sialoglycoproteins; SPP1 ...
Sialoglycoproteins. 2. 1999. 414. 0.080. Why? Osteopontin. 2. 1999. 341. 0.080. Why? ...
Gel filtration of sialoglycoproteins Biochem J (July,1978). A specific binding of the cholecystokinin-releasing peptide ( ...
1988) Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins ...
The interaction of wheat germ agglutinin with sialoglycoproteins. J. Biol. Chem. 254:4000-4008. ...
The identified sialoglycoproteins include those involved in calcium signaling in synapses such as Syt2, supporting the ... The newly synthesized sialoglycoproteins associated with synapses contribute, at least partially, to the newly synthesized ... B) Evaluation of six labeling protocols for metabolic incorporation of azides into brain sialoglycoproteins. Mice (n = 3 per ... D) Cellular localizations enriched in the newly synthesized sialoglycoproteins identified in the LP-9AzSia-treated brain. The ...
Identification of novel tumor-associated cell surface sialoglycoproteins in human glioblastoma tumors using quantitative ...
Targeted identification of metastasis-associated cell-surface sialoglycoproteins in prostate cancer.. Yang L, Nyalwidhe JO, Guo ...
Rabinowitz, S., and Gordon, S. (1989). Differential expression of membrane sialoglycoproteins in exudate and resident mouse ...
2013). Sialoglycoproteins and N-glycans from secreted exosomes of ovarian carcinoma cells. PLoS ONE 8:e78631. doi: 10.1371/ ...
Sialoglycoproteins in morphological distinct stages of Mucor polymorphosporus and their influence on phagocytosis by human ...
  • Correlation of retinoic acid-enhanced sialyltransferase activity and glycosylation of specific cell surface sialoglycoproteins with growth inhibition in a murine melanoma cell system. (semanticscholar.org)
  • Retinoic acid inhibits the proliferation of the murine melanoma clone S91-C-2 cells, enhances the glycosylation of specific cell surface sialoglycoproteins, and stimulates sialytransferase activity. (semanticscholar.org)
  • this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. (wikipedia.org)
  • Anstee, D. J., Ridgwell, K., Tanner, M. J. A., Daniels, G. L. & Parsons, S. F. (1984): Individuals lacking the Gerbich blood-group antigen have alterations in the human erythrocyte membrane sialoglycoproteins β and y. (springer.com)
  • Dahr, W. (1986): Immunochemistry of sialoglycoproteins in human red blood cell membranes. (springer.com)
  • This change was shown to be due to a generalised loss of sialic acid from all of the major membrane sialoglycoproteins. (gla.ac.uk)
  • Chayen A, Avron B, Nuchamowitz Y, Mirelman D (1988) Appearance of sialoglycoproteins in encysting cells of E. histolytica. (biology-online.org)
  • Our approach effectively targeted surface sialoglycoproteins and efficiently identified proteins that underlie the metastatic potential of the ML2 cells. (plu.mx)
  • A selective enrichment of sialoglycoproteins was confirmed. (plu.mx)
  • Inhibition experiments with fetuin, a 1-acid glycoprotein and N-acetyl neuraminic acid confirmed the specificity of bacterial binding to sialoglycoproteins. (uni-wuerzburg.de)
  • Na login bij de Digital Library sluit het venster en refresh (F5) de Hydrotheek pagina. (wur.nl)
  • Targeted identification of metastasis-associated cell-surface sialoglycoproteins in prostate cancer. (plu.mx)
  • Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure. (visualsonics.com)
  • ii) Of the total Neu5Gc in the Sia compounds that the cells synthesized, 90% was found in the secreted sialoglycoproteins, whereas for Neu5Ac, 70% was found in the secreted sialoglycoproteins. (scialert.net)
  • Treatment of intact red cells with a variety of proteolytic enzymes produced n new component of molecular weight 61-63,000 and also degraded component 3 and the membrane sialoglycoproteins. (gla.ac.uk)
  • Cloning of lysosomal sialoglycoproteins and their expression in cells. (nii.ac.jp)
  • iii) The Neu5Gc ratio was higher in the secreted sialoglycoproteins (as high as 40% of total Sias) than in intracellular sialoglycoproteins. (scialert.net)
  • We have combined glycan metabolic labeling with mass spectrometry analysis to identify and characterize metastasis-associated cell surface sialoglycoproteins. (plu.mx)
  • In this study, sialoglycoproteins (ovomucin, ovomucoid, ovoflavoprotein and ovoglycoprotein) were prepared from hen' s egg white and their biological activities (antitumor activities against SR-180 and MH134 cell, and inhibitory activities of hemagglutination by rotavirus and newcastle disease virus) were determined. (nii.ac.jp)
  • Plant proteins with high affinity to specific cellular glycoconjugates: Lectin I (D-galactose-and N-acetyl-D-galactosamine, and sialoglycoproteins specific lectin) 37 , 38 , 39 , 40 , 41 , Lectin II and III (N-acetyl-D-galactosamine specific proteins) 9 . (sigmaaldrich.com)
  • Ovomucin was found to have the specific higher activities among the used sialoglycoproteins. (nii.ac.jp)
  • The major and minor sialoglycoproteins of the bovine erythrocyte have been solubilized and extensively purified. (elsevier.com)
  • Over expression of 9- O- acetylated sialoglycoproteins (Neu5,9Ac 2 -GPs, abbreviated as O AcSGP) has been demonstrated as a disease-associated antigen on the lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). (beds.ac.uk)
  • iv) The majority of the secreted sialoglycoproteins was anchored on the culture dishes and solubilized by brief trypsin treatment. (scialert.net)
  • Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix. (wur.nl)