A family of serine proteinase inhibitors which are similar in amino acid sequence and mechanism of inhibition, but differ in their specificity toward proteolytic enzymes. This family includes alpha 1-antitrypsin, angiotensinogen, ovalbumin, antiplasmin, alpha 1-antichymotrypsin, thyroxine-binding protein, complement 1 inactivators, antithrombin III, heparin cofactor II, plasminogen inactivators, gene Y protein, placental plasminogen activator inhibitor, and barley Z protein. Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in plants where their function is not known.
Exogenous or endogenous compounds which inhibit SERINE ENDOPEPTIDASES.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
Glycoprotein found in alpha(1)-globulin region in human serum. It inhibits chymotrypsin-like proteinases in vivo and has cytotoxic killer-cell activity in vitro. The protein also has a role as an acute-phase protein and is active in the control of immunologic and inflammatory processes, and as a tumor marker. It is a member of the serpin superfamily.
An enzyme that catalyzes the hydrolysis of proteins, including elastin. It cleaves preferentially bonds at the carboxyl side of Ala and Val, with greater specificity for Ala. EC 3.4.21.37.
A member of the serpin family of proteins that is found in plasma and urine. It is dependent on heparin and is able to inhibit activated PROTEIN C; THROMBIN; KALLIKREIN; and other SERINE ENDOPEPTIDASES.
Serum proteins that inhibit, antagonize, or inactivate COMPLEMENT C1 or its subunits.
Extracellular protease inhibitors that are secreted from FIBROBLASTS. They form a covalent complex with SERINE PROTEASES and can mediate their cellular internalization and degradation.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.
A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC 3.4.21.36.
Endogenous factors and drugs that directly inhibit the action of THROMBIN, usually by blocking its enzymatic activity. They are distinguished from INDIRECT THROMBIN INHIBITORS, such as HEPARIN, which act by enhancing the inhibitory effects of antithrombins.
A protease nexin and serpin subtype that is specific for several SERINE PROTEASES including UROKINASE; THROMBIN; TRYPSIN; and PLASMINOGEN ACTIVATORS.
A serine protease found in the azurophil granules of NEUTROPHILS. It has an enzyme specificity similar to that of chymotrypsin C.
A member of the serpin superfamily found in plasma that inhibits the lysis of fibrin clots which are induced by plasminogen activator. It is a glycoprotein, molecular weight approximately 70,000 that migrates in the alpha 2 region in immunoelectrophoresis. It is the principal plasmin inactivator in blood, rapidly forming a very stable complex with plasmin.
A species of ORTHOPOXVIRUS that is the etiologic agent of COWPOX. It is closely related to but antigenically different from VACCINIA VIRUS.
A plasma alpha 2 glycoprotein that accounts for the major antithrombin activity of normal plasma and also inhibits several other enzymes. It is a member of the serpin superfamily.
A group of seed storage proteins restricted to the POACEAE family. They are rich in GLUTAMINE and PROLINE.
A cytastin subtype found at high levels in the SKIN and in BLOOD CELLS. Cystatin A incorporates into the cornified cell envelope of stratified squamous epithelial cells and may play a role in bacteriostatic properties of skin.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The blood/lymphlike nutrient fluid of some invertebrates.
A sulfated plasma protein with a MW of approximately 66kDa that resembles ANTITHROMBIN III. The protein is an inhibitor of thrombin in plasma and is activated by dermatan sulfate or heparin. It is a member of the serpin superfamily.
A member of the serpin family of proteins. It inhibits both the tissue-type and urokinase-type plasminogen activators.
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
Serine proteinase inhibitors which inhibit trypsin. They may be endogenous or exogenous compounds.
A family of serine endopeptidases found in the SECRETORY GRANULES of LEUKOCYTES such as CYTOTOXIC T-LYMPHOCYTES and NATURAL KILLER CELLS. When secreted into the intercellular space granzymes act to eliminate transformed and virus-infected host cells.
Proteins prepared by recombinant DNA technology.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. EC 1.10.3.1.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
A group of lysosomal proteinases or endopeptidases found in aqueous extracts of a variety of animal tissues. They function optimally within an acidic pH range. The cathepsins occur as a variety of enzyme subtypes including SERINE PROTEASES; ASPARTIC PROTEINASES; and CYSTEINE PROTEASES.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A plant genus of the family CUCURBITACEAE, order Violales, subclass Dilleniidae, which includes pumpkin, gourd and squash.
The rate dynamics in chemical or physical systems.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
A genus of beetles which infests grain products. Its larva is called mealworm.
A family of neutral serine proteases with CHYMOTRYPSIN-like activity. Chymases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.
Member of the serpin family of proteins. It inhibits both the tissue-type and urokinase-type plasminogen activators.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
Synthetic or naturally occurring substances related to coumarin, the delta-lactone of coumarinic acid.
Living outdoors as a recreational activity.
The enhancement of physical, cognitive, emotional and social skills so an individual may participate in chosen activities. Recreational modalities are used in designed intervention strategies, incorporating individual's interests to make the therapy process meaningful and relevant.
Creation and development of bodies within solar systems, includes study of early planetary geology.
Planet that is the third in order from the sun. It is one of the four inner or terrestrial planets of the SOLAR SYSTEM.

MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member. (1/1699)

Terminal cell differentiation is correlated with the extensive sequestering of previously active genes into compact transcriptionally inert heterochromatin. In vertebrate blood cells, these changes can be traced to the accumulation of a developmentally regulated heterochromatin protein, MENT. Cryoelectron microscopy of chicken granulocyte chromatin, which is highly enriched with MENT, reveals exceptionally compact polynucleosomes, which maintain a level of higher order folding above that imposed by linker histones. The amino acid sequence of MENT reveals a close structural relationship with serpins, a large family of proteins known for their ability to undergo dramatic conformational transitions. Conservation of the "hinge region" consensus in MENT indicates that this ability is retained by the protein. MENT is distinguished from the other serpins by being a basic protein, containing several positively charged surface clusters, which are likely to be involved in ionic interactions with DNA. One of the positively charged domains bears a significant similarity to the chromatin binding region of nuclear lamina proteins and with the A.T-rich DNA-binding motif, which may account for the targeting of MENT to peripheral heterochromatin. MENT ectopically expressed in a mammalian cell line is transported into nuclei and is associated with intranuclear foci of condensed chromatin.  (+info)

The intracellular serpin proteinase inhibitor 6 is expressed in monocytes and granulocytes and is a potent inhibitor of the azurophilic granule protease, cathepsin G. (2/1699)

The monocyte and granulocyte azurophilic granule proteinases elastase, proteinase 3, and cathepsin G are implicated in acute and chronic diseases thought to result from an imbalance between the secreted proteinase(s) and circulating serpins such as alpha1-proteinase inhibitor and alpha1-antichymotrypsin. We show here that the intracellular serpin, proteinase inhibitor 6 (PI-6), is present in monocytes, granulocytes, and myelomonocytic cell lines. In extracts from these cells, PI-6 bound an endogenous membrane-associated serine proteinase to form an sodium dodecyl sulfate (SDS)-stable complex. Using antibodies to urokinase, elastase, proteinase 3, or cathepsin G, we demonstrated that the complex contains cathepsin G. Native cathepsin G and recombinant PI-6 formed an SDS-stable complex in vitro similar in size to that observed in the extracts. Further kinetic analysis demonstrated that cathepsin G and PI-6 rapidly form a tight 1:1 complex (ka = 6.8 +/- 0.2 x 10(6) mol/L-1s-1 at 17 degrees C; Ki = 9.2 +/- 0.04 x 10(-10) mol/L). We propose that PI-6 complements alpha1-proteinase inhibitor and alpha1-antichymotrypsin (which control extracellular proteolysis) by neutralizing cathepsin G that leaks into the cytoplasm of monocytes or granulocytes during biosynthesis or phagocytosis. Control of intracellular cathepsin G may be particularly important, because it has recently been shown to activate the proapoptotic proteinase, caspase-7.  (+info)

Role for caspase-mediated cleavage of Rad51 in induction of apoptosis by DNA damage. (3/1699)

We report here that the Rad51 recombinase is cleaved in mammalian cells during the induction of apoptosis by ionizing radiation (IR) exposure. The results demonstrate that IR induces Rad51 cleavage by a caspase-dependent mechanism. Further support for involvement of caspases is provided by the finding that IR-induced proteolysis of Rad51 is inhibited by Ac-DEVD-CHO. In vitro studies show that Rad51 is cleaved by caspase 3 at a DVLD/N site. Stable expression of a Rad51 mutant in which the aspartic acid residues were mutated to alanines (AVLA/N) confirmed that the DVLD/N site is responsible for the cleavage of Rad51 in IR-induced apoptosis. The functional significance of Rad51 proteolysis is supported by the finding that, unlike intact Rad51, the N- and C-terminal cleavage products fail to exhibit recombinase activity. In cells, overexpression of the Rad51(D-A) mutant had no effect on activation of caspase 3 but did abrogate in part the apoptotic response to IR exposure. We conclude that proteolytic inactivation of Rad51 by a caspase-mediated mechanism contributes to the cell death response induced by DNA damage.  (+info)

Bcl-xL blocks activation of related adhesion focal tyrosine kinase/proline-rich tyrosine kinase 2 and stress-activated protein kinase/c-Jun N-terminal protein kinase in the cellular response to methylmethane sulfonate. (4/1699)

The stress-activated protein kinase/c-Jun N-terminal protein kinase (JNK) is induced in response to ionizing radiation and other DNA-damaging agents. Recent studies indicate that activation of JNK is necessary for induction of apoptosis in response to diverse agents. Here we demonstrate that methylmethane sulfonate (MMS)-induced activation of JNK is inhibited by overexpression of the anti-apoptotic protein Bcl-xL, but not by caspase inhibitors CrmA and p35. By contrast, UV-induced JNK activity is insensitive to Bcl-xL. The results demonstrate that treatment with MMS is associated with an increase in tyrosine phosphorylation of related adhesion focal tyrosine kinase (RAFTK)/proline-rich tyrosine kinase 2 (PYK2), an upstream effector of JNK and that this phosphorylation is inhibited by overexpression of Bcl-xL. Furthermore, overexpression of a dominant-negative mutant of RAFTK (RAFTK K-M) inhibits MMS-induced JNK activation. The results indicate that inhibition of RAFTK phosphorylation by MMS in Bcl-xL cells is attributed to an increase in tyrosine phosphatase activity in these cells. Hence, treatment of Bcl-xL cells with sodium vanadate, a tyrosine phosphatase inhibitor, restores MMS-induced activation of RAFTK and JNK. These findings indicate that RAFTK-dependent induction of JNK in response to MMS is sensitive to Bcl-xL, but not to CrmA and p35, by a mechanism that inhibits tyrosine phosphorylation and thereby activation of RAFTK. Taken together, these findings support a novel role for Bcl-xL that is independent of the caspase cascade.  (+info)

Ligand binding properties of the very low density lipoprotein receptor. Absence of the third complement-type repeat encoded by exon 4 is associated with reduced binding of Mr 40,000 receptor-associated protein. (5/1699)

The very low density lipoprotein receptor (VLDLR) binds, among other ligands, the Mr 40,000 receptor-associated protein (RAP) and a variety of serine proteinase-serpin complexes, including complexes of the proteinase urokinase-type plasminogen activator (uPA) with the serpins plasminogen activator inhibitor-1 (PAI-1) and protease nexin-1 (PN-1). We have analyzed the binding of RAP, uPA.PAI-1, and uPA.PN-1 to two naturally occurring VLDLR variants, VLDLR-I, containing all eight complement-type repeats, and VLDLR-III, lacking the third complement-type repeat, encoded by exon 4. VLDLR-III displayed approximately 4-fold lower binding of RAP than VLDLR-I and approximately 10-fold lower binding of the most C-terminal one of the three domains of RAP. In contrast, the binding of uPA.PAI-1 and uPA.PN-1 to the two VLDLR variants was indistinguishable. Surprisingly, uPA.PN-1, but not uPA.PAI-1, competed RAP binding to both VLDLR variants. These observations show that the third complement-type repeat plays a crucial role in maintaining the contact sites needed for optimal recognition of RAP, but does not affect the proteinase-serpin complex contact sites, and that two ligands can show full cross-competition without sharing the same contacts with the receptor. These results elucidate the mechanisms of molecular recognition of ligands by receptors of the low density lipoprotein receptor family.  (+info)

Suppression of breast cancer growth and metastasis by a serpin myoepithelium-derived serine proteinase inhibitor expressed in the mammary myoepithelial cells. (6/1699)

A serpin was identified in normal mammary gland by differential cDNA sequencing. In situ hybridization has detected this serpin exclusively in the myoepithelial cells on the normal and noninvasive mammary epithelial side of the basement membrane and thus was named myoepithelium-derived serine proteinase inhibitor (MEPI). No MEPI expression was detected in the malignant breast carcinomas. MEPI encodes a 405-aa precursor, including an 18-residue secretion signal with a calculated molecular mass of 46 kDa. The predicted sequence of the new protein shares 33% sequence identity and 58% sequence similarity to plasminogen activator inhibitor (PAI)-1 and PAI-2. To determine whether MEPI can modulate the in vivo growth and progression of human breast cancers, we transfected a full-length MEPI cDNA into human breast cancer cells and studied the orthotopic growth of MEPI-transfected vs. control clones in the mammary fat pad of athymic nude mice. Overexpression of MEPI inhibited the invasion of the cells in the in vitro invasion assay. When injected orthotopically into nude mice, the primary tumor volumes, axillary lymph node metastasis, and lung metastasis were significantly inhibited in MEPI-transfected clones as compared with controls. The expression of MEPI in myoepithelial cells may prevent breast cancer malignant progression leading to metastasis.  (+info)

Regulation of pro-apoptotic leucocyte granule serine proteinases by intracellular serpins. (7/1699)

Caspase activation and apoptosis can be initiated by the introduction of serine proteinases into the cytoplasm of a cell. Cytotoxic lymphocytes have evolved at least one serine proteinase with specific pro-apoptotic activity (granzyme B), as well as the mechanisms to deliver it into a target cell, and recent evidence suggests that other leucocyte granule proteinases may also have the capacity to kill if released into the interior of cells. For example, the monocyte/granulocyte proteinase cathepsin G can activate caspases in vitro, and will induce apoptosis if its entry into cells is mediated by a bacterial pore-forming protein. The potent pro-apoptotic activity of granzyme B and cathepsin G suggests that cells producing these (or other) proteinases would be at risk from self-induced death if the systems involved in packaging, degranulation or targeting fail and allow proteinases to enter the host cell cytoplasm. The purpose of the present review is to describe recent work on a group of intracellular serine proteinase inhibitors (serpins) which may function in leucocytes to prevent autolysis induced by the granule serine proteinases.  (+info)

Anti-viral strategies of cytotoxic T lymphocytes are manifested through a variety of granule-bound pathways of apoptosis induction. (8/1699)

Cytotoxic T cells and natural killer cells together constitute a major defence against virus infection, through their ability to induce apoptotic death in infected cells. These cytolytic lymphocytes kill their targets through two principal mechanisms, and one of these, granule exocytosis, is essential for an effective in vivo immune response against many viruses. In recent years, the authors and other investigators have identified several distinct mechanisms that can induce death in a targeted cell. In the present article, it is postulated that the reason for this redundancy of lethal mechanisms is to deal with the array of anti-apoptotic molecules elaborated by viruses to extend the life of infected cells. The fate of such a cell therefore reflects the balance of pro-apoptotic (immune) and anti-apoptotic (viral) strategies that have developed over eons of evolutionary time.  (+info)

Squamous cell carcinoma antigen: a role in the early identification of nodal metastases in men with squamous cell carcinoma of the penis. To evaluate whether serum squamous cell carcinoma antigen (SCCAg) measurements may be of use in identifying nodal metastases in patients with SCC of the penis after treating the primary tumour. The levels of SCCAg were analysed in 11 men with penile SCC between 1994 and 2001. An elevated SCCAg level had a sensitivity of 57% (95% confidence interval, CI, 18-90%) and a specificity of 100% (CI 40-100%) for nodal metastases. Levels of SCCAg increased exponentially in patients who developed nodal metastases after treatment of the primary tumour, and were elevated before clinical or radiological evidence of nodal disease. Either the absolute level or the rate of rise of SCCAg may be a useful tool with which to follow patients after excision of the primary tumour. It may be more sensitive than computed tomography and magnetic resonanc imaging in detecting recurrence, ...
TY - JOUR. T1 - Protective role of small pigment epithelium-derived factor (PEDF) peptide in diabetic renal injury. AU - Awad, Alaa S.. AU - Gao, Ting. AU - Gvritishvili, Anzor. AU - You, Hanning. AU - Liu, Yanling. AU - Cooper, Timothy K.. AU - Brian Reeves, W.. AU - Tombran-Tink, Joyce. PY - 2013/9/15. Y1 - 2013/9/15. N2 - Pigment epithelium-derived factor (PEDF) is a multifunctional protein with antiangiogenic, antioxidative, and anti-inflammatory properties. PEDF is involved in the pathogenesis of diabetic retinopathy, but its direct role in the kidneys remains unclear. We hypothesize that a PEDF fragment (P78-PEDF) confers kidney protection in diabetic nephropathy (DN). The localization of the full-length PEDF protein were determined in DBA mice following multiple low doses of streptozotocin. Using immunohistochemistry, PEDF was localized in the kidney vasculature, interstitial space, glomeruli, tubules, and renal medulla. Kidney PEDF protein and mRNA expression were significantly reduced ...
TY - JOUR. T1 - Gene structure, chromosomal localization, and expression of the murine homologue of human proteinase inhibitor 6 (PI-6) suggests divergence of PI-6 from the ovalbumin serpins. AU - Sun, J.. AU - Rose, J. B.. AU - Bird, P.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - Human proteinase inhibitor 6 (PI-6) is a recently described protein belonging to the serine proteinase inhibitor (serpin) superfamily. Sequence similarity suggests that PI-6 most resembles the ovalbumin (ov) serpins which include plasminogen activator inhibitor-2, the squamous cell carcinoma antigen, monocyte/neutrophil elastase inhibitor, and maspin. Although these proteins are associated with carcinomas and inflammation, they appear to have diverse functions and little is known of their physiological roles. In this study we have characterized cDNA and genomic clones encoding mouse PI-6 in order to analyze the localization, structure, and expression of the gene. The reactive center residues (Arg-Cys) are conserved in the ...
TY - JOUR. T1 - C1q/TNF-related protein-3 (CTRP-3) and pigment epithelium-derived factor (PEDF) concentrations in patients with type 2 diabetes and metabolic syndrome. AU - Choi, Kyung Mook. AU - Hwang, Soon Young. AU - Hong, Ho Cheol. AU - Yang, Sae Jeong. AU - Choi, Hae Yoon. AU - Yoo, Hye Jin. AU - Lee, Kwan Woo. AU - Nam, Moon Suk. AU - Park, Yong Soo. AU - Woo, Jeong Taek. AU - Kim, Young Seol. AU - Choi, Dong Seop. AU - Youn, Byung Soo. AU - Baik, Sei Hyun. N1 - Copyright: Copyright 2013 Elsevier B.V., All rights reserved.. PY - 2012/11. Y1 - 2012/11. N2 - Recent studies have suggested that a novel adipokine, C1q/tumor necrosis factor-related protein-3 (CTRP-3), a paralog of adiponectin, may play an important role in the regulation of glucose metabolism and innate immunity. Pigment epithelium-derived factor (PEDF), a multifunctional protein with antioxidant and anti-inflammatory properties, is associated with insulin resistance and metabolic syndrome. We examined circulating CTRP-3 and ...
Our analysis of cervical cancer patients treated with CCRT indicated that the sensitivity and specificity of two consecutive increases in serum SCC-Ag for predicting tumor recurrence were 61.1% and 97.9%, respectively. These results are comparable to previously reported results. For example, several studies of cervical cancer patients showed that an elevated serum SCC-Ag level was associated with 70-92% rate of recurrent tumors [8, 11, 14-19]; in addition, the specificity of SCC-Ag during the follow-up period was quite high, varying from 95 to 98% [7, 16, 20]. According to our ROC analysis, the area under the ROC curve indicated the ΔSCC-Ag was 0.83 for patients who had elevated pre-treatment SCC-Ag. Therefore, our results indicate that ΔSCC-Ag had good clinical performance in detection of recurrent disease.. As described above, we defined biochemical failure as two consecutive SCC-Ag values above normal. There are no standard criteria used to define biochemical failure in cervical cancer, and ...
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic progressive disorder with a poor prognosis. Epithelial instability is a crucial step in the development and progression of the disease, including neoplastic transformation. Few tissue markers for epithelial instability have been reported in IPF. Squamous cell carcinoma antigen (SCCA) is a serine protease inhibitor typically expressed by dysplastic and neoplastic cells of epithelial origin, more often in squamous cell tumours. At present, no information is available on its expression in IPF. METHODS: SCCA and transforming growth factor beta (TGFbeta) expression in surgical lung biopsies from 22 patients with IPF and 20 control cases was examined. An in vitro study using A549 pneumocytes was also conducted to investigate the relationship between SCCA and TGFbeta expression. SCCA and TGFbeta epithelial expression was evaluated by immunohistochemistry and reverse transcription-PCR (RT-PCR). SCCA values were correlated with different ...
Purpose: : To determine the effect of matrix metalloproteinase (MMP)-mediated proteolysis on the anti-angiogenic activity of pigment epithelium-derived factor (PEDF) in vitro and determine the MMP-mediated cleavage sites within the primary sequence of PEDF. Furthermore, we have determined the effect of inhibiting MMPs on the intraocular levels of PEDF protein expressed from an adenoviral expression system in a mouse eye. Methods: : To determine the site at which PEDF is cleaved by MMP-9, purified PEDF was incubated with and without MMP-9 (1:200 mass ratio MMP to PEDF) at 37°C for 24h then with trypsin (1:40 trypsin to PEDF) overnight. The peptides were separated by reverse-phase HPLC on a C-18 column, collected, treated with 2-sulfobenzoic acid anhydride, and sequenced by MALDI-MS. The anti-angiogenic activity of MMP-treated PEDF was quantified using the mouse aortic ring bioassay and a standard invasion assay. The effect of MMP inhibition on PEDF levels was evaluated by immunoassay 1 day after ...
The integrity of the vascular barrier, which is essential to blood vessel homoeostasis, can be disrupted by a variety of soluble permeability factors during sepsis. Pigment epithelium-derived factor (PEDF), a potent endogenous anti-angiogenic molecule, is significantly increased in sepsis, but its role in endothelial dysfunction has not been defined. To assess the role of PEDF in the vasculature, we evaluated the effects of exogenous PEDF in vivo using a mouse model of cecal ligation and puncture (CLP)-induced sepsis and in vitro using human dermal microvascular endothelial cells (HDMECs). In addition, PEDF was inhibited using a PEDF-monoclonal antibody (PEDF-mAb) or recombinant lentivirus vectors targeting PEDF receptors, including adipose triglyceride lipase (ATGL) and laminin receptor (LR). Our results showed that exogenous PEDF induced vascular hyperpermeability, as measured by extravasation of Evans Blue (EB), dextran and microspheres in the skin, blood, trachea and cremaster muscle, both ...
Purpose: : It has been reported that Pigment Epithelium-Derived Factor (PEDF) is a potent anti-angiogenic factor, whose concentration in the vitreous is reported to be around 1500 ng/ml. However, the precise mechanisms are not clarified to date. Therefore, we tried to examine how PEDF has suppressive effects on neovascularization by way of in vitro assays. Methods: : Bovine retinal microvascular endothelial cells (BRECs) and human umbilical vein endothelial cells (HUVECs) were used for in vitro assays. To estimate the effect of PEDF on the proliferation, migration and tube formation by endothelial cells, thymidine uptake, boyden chamber assay and tube formation assay were performed. Moreover, to investigate whether PEDF suppresses the expression of vascular endothelial growth factor (VEGF) or its receptor (VEGFR2) and inhibits VEGF-dependent phosphorylation of VEGFR2 or p44/42 MAP kinase, Northern and Western blot analyses were performed. SU5416 was used as a selective inhibitor of VEGFR2 ...
OBJECTIVE: Pigment epithelium-derived factor (PEDF) is an adipocyte-secreted factor involved in the development of insulin resistance in obesity. Previous studies have identified PEDF as a regulator of triacylglycerol metabolism in the liver that may act through adipose triglyceride lipase (ATGL). We used ATGL(-/-) mice to determine the role of PEDF in regulating lipid and glucose metabolism. RESEARCH DESIGN AND METHODS: Recombinant PEDF was administered to ATGL(-/-) and wild-type mice, and whole-body energy metabolism was studied by indirect calorimetry. Adipose tissue lipolysis and skeletal muscle fatty acid metabolism was determined in isolated tissue preparations. Muscle lipids were assessed by electrospray ionization-tandem mass spectrometry. Whole-body insulin sensitivity and skeletal muscle glucose uptake were assessed. RESULTS: PEDF impaired the capacity to adjust substrate selection, resulting in a delayed diurnal decline in the respiratory exchange ratio, and suppressed daily fatty ...
Introduction Pigment epithelium-derived factor (PEDF) is a 50kDa, secreted glycoprotein that has been identified as a member of the serpin gene family and has been shown to exhibit neurotrophic, neuroprotective, anti-angiogenic and anti-tumourigenic effects. The aims of our study were to determine the expression profile of PEDF in a range of colorectal cell lines and its association with clinical and pathological data. Method Six human cell lines (RKO and HT115 are colonic adenocarcinoma, HRT-18 is rectal adenocarcinoma, COLO-201 is metastatic adenocarcinoma (originating from ascites), LS174T is a mucinous adenocarcinoma, and CCD-33C0 is a normal colorectal fibroblast cell line) were analysed using polymerase chain reaction (PCR) and quantitative transcript analysis (qPCR). Primary colorectal cancer tissue was collected at operation and analysed using qPCR. Results PEDF transcript was positive in RKO, HRT-18, LS174T and CCD-33C0 cell lines but negative in HT115 and COLO-201. On qPCR, PEDF ...
Increased production of reactive oxygen species (ROS) and inflammation are major contributors to the development and progression of diabetes-associated erectile dysfunction (DMED). As an endogenous antioxidant and anti-inflammatory factor, the potential implication of pigment epithelium-derived factor (PEDF) in DMED has not been revealed. To assess the potential antioxidant and anti-inflammatory functions of PEDF in DMED, we first demonstrated that PEDF was significantly decreased at the levels of the mRNA and protein in the penis of diabetic rats compared with normal controls. To test the hypothesis that decreased the penile levels of PEDF are associated with oxidative stress and inflammation in DMED, an adenovirus expressing PEDF (Ad-PEDF) or the same titer of control virus (Ad-GFP) was intracavernously administered at 2 weeks after diabetic onset. After 6 weeks of treatment, we found that administration of Ad-PEDF could significantly increase erectile response to cavernosal nerve stimulation ...
We here suggest that pigment epithelium-derived factor (PEDF) does not have an effect on lesion size, behavioral outcome, cell proliferation, or cell death after striatal ischemia in the mouse. PEDF is a neurotrophic factor with neuroprotective, antiangiogenic, and antipermeability effects. It influences self-renewal of neural stem cells and proliferation of microglia. We investigated whether intraventricular infusion of PEDF reduces infarct size and cell death, ameliorates behavioral outcome, and influences cell proliferation in the one-hour middle cerebral artery occlusion (MCAO) mouse model of focal cerebral ischemia. C57Bl6/N mice were implanted with PEDF or artificial cerebrospinal fluid (control) osmotic pumps and subjected to 60-minute MCAO 48 hours after pump implantation. They received daily BrdU injections for 7 days after MCAO in order to investigate cell proliferation. Infarct volumes were determined 24 hours after reperfusion using magnetic resonance imaging. We removed the pumps on day 5
Pigment Epithelium-Derived Factor: Chemistry, Structure, Biology, and Applications - A special issue journal published by Hindawi
Pigment epithelium-derived factor (PEDF) also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functions. Found in vertebrates, this 50 kDa protein is being researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization, heart disease, and cancer. In humans, pigment epithelium-derived factor is encoded by the SERPINF1 gene. Pigment epithelium-derived factor (PEDF) was originally discovered by Joyce Tombran-Tink and Lincoln Johnson in the late 1980s. This group was studying human retinal cell development by identifying secreted factors produced by the retinal pigmented epithelium (RPE), a layer of cells that supports the retina. Upon noticing RPE produced a factor that promoted the differentiation of primitive retinal cells into cells of a neuronal phenotype, they set out to determine the identity of the factor. They isolated proteins unique to RPE cells and tested the ...
The serpin superfamily is characterized by proteins that fold into a conserved tertiary structure and exploits a sophisticated and irreversible suicide-mechanism of inhibition. Vertebrate serpins are classified into six groups (V1-V6), based on three independent biological features-genomic organization, diagnostic amino acid sites and rare indels. However, this classification system was based on the limited number of mammalian genomes available. In this study, several non-mammalian genomes are used to validate this classification system using the powerful Bayesian phylogenetic method. This method supports the intron and indel based vertebrate classification and proves that serpins have been maintained from lampreys to humans for about 500 MY. Lampreys have fewer than 10 serpins, which expand into 36 serpins in humans. The two expanding groups V1 and V2 have SERPINB1/SERPINB6 and SERPINA8/SERPIND1 as the ancestral serpins, respectively. Large clusters of serpins are formed by local duplications of these
We investigated the significance of high- mobility group box1 (HMGB1) and T-cell-mediated immunity and prognostic value in cervical cancer. HMGB1, forkhead/winged helix transcription factor p3 (Foxp3), IL-2, and IL-10 protein expression was analyzed in 100 cervical tissue samples including cervical cancer, cervical intraepithelial neoplasia (CIN), and healthy control samples using immunohistochemistry. Serum squamous cell carcinoma antigen (SCC-Ag) was immunoradiometrically measured in 32 serum samples from 37 cases of squamous cervical cancer. HMGB1 and SCC-Ag were then correlated to clinicopathological characteristics. HMGB1 expression tends to increase as cervical cancer progresses and it was found to be significantly correlated to FIGO stage and lymph node metastasis. These findings suggest that HMGB1 may be a useful prognostic indicator of cervical carcinoma. In addition, there were significant positive relationships between HMGB1 and FOXP3 or IL-10 expression (both p | 0.05). In contrast, HMGB1
A 55-year-old female presented with abnormal vaginal bleeding and was diagnosed with FIGO Stage IIA (T2aN0M0, UICC 6th edition) squamous cell carcinoma of the uterine cervix. The patient had an elevated value of serum squamous cell carcinoma antigen (SCC), 5.3 ng/ml (normal value: 0-1.5 ng/ml). The patient was treated with external beam RT (EBRT) of 50 Gy in 25 fractions to the pelvis using conventional X-rays (consisting of 26 Gy of whole pelvis fields and the last 24 Gy of center shielding pelvis fields) and 4 fractions of high-dose-rate (HDR) intracavitary brachytherapy (BT) with the total dose of 24 Gy given to point A. There were no acute adverse effects greater than Grade 3. However, there were Grade 2 upper gastrointestinal symptoms and Grade 1 neutropenia. The tumor completely disappeared, and the serum SCC levels returned to normal after therapy. Therefore, the patient received no further treatment. Twenty-three months later, the patient developed bilateral edema in the legs. A ...
hypothetical protein, alpha-2 antiplasmin, Alpha-2-antiplasmin, alpha-2 antiplasmin, pigment epithelium derived factor, AS27_04276, caspin, CB1_000765141, cell proliferation-inducing gene 35 protein, Dmrs91, EPC-1, GW7_05089, H920_13024, I79_024527, M959_13429, MDA_GLEAN10016889, N300_03473, N302_16382, N305_15082, N306_10474, N307_07373, N308_00896, N309_12586, N311_08061, N312_04929, N320_07386, N321_02844, N322_05786, N324_04587, N325_07445, N326_06640, N327_07162, N329_08729, N330_08014, N332_10702, N333_06723, N335_06305, N336_08169, N339_06481, N340_07977, N341_07059, OI12, OI6, PAL_GLEAN10020010, PANDA_006472, PEDF, Pedfl, PIG35, pigment epithelium derived factor, pigment epithelium-derived factor, pigment epithelium-derived factor-like protein, Pigment epithelium-derived factor precursor-like protein, Sdf3, SDF-3, serine (or cysteine) peptidase inhibitor, clade F, member 1, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member ...
Centerin [SERPINA9/GCET1 (germinal centre B-cell-expressed transcript 1)] is a serpin (serine protease inhibitor) whose expression is restricted to germinal centre B-cells and lymphoid malignancies with germinal centre B-cell maturation. Expression of centerin, together with bcl-6 and GCET2, constitutes a germinal centre B-cell signature, which is associated with a good prognosis in diffuse large B-cell lymphomas, but the molecular basis for this remains to be elucidated. We report here the cloning, expression and molecular characterization of bacterial recombinant centerin. Biophysical studies demonstrated that centerin was able to undergo the stressed to relaxed conformational change which is an absolute requirement for protease inhibitory activity. Kinetic analysis showed that centerin rapidly inhibited the serine protease trypsin (ka=1.9×105 M−1·s−1) and also demonstrated measurable inhibition of thrombin (ka=1.17×103 M−1·s−1) and plasmin (ka=1.92×103 M−1·s−1). Centerin ...
abstract = {Members of the serpin superfamily of proteins have been found in all living organisms, although rarely in bacteria or fungi. They have been extensively studied in mammals, where many rapid physiological responses are regulated by inhibitory serpins. In addition to the inhibitory serpins, a large group of noninhibitory proteins with a conserved serpin fold have also been identified in mammals. These noninhibitory proteins have a wide range of functions, from storage proteins to molecular chaperones, hormone transporters, and tumor suppressors. In contrast, until recently, very little was known about insect serpins in general, or Drosophila serpins in particular. In the last decade, however, there has been an increasing interest in the serpin biology of insects. It is becoming clear that, like in mammals, a similar wide range of physiological responses are regulated in insects and that noninhibitory serpin-fold proteins also play key roles in insect biology. Drosophila is also an ...
OPUS (Open Publications of UTS Scholars) is the UTS institutional repository. It showcases the research of UTS staff and postgraduate students to a global audience. For you, as a researcher, OPUS increases the visibility and accessibility of your research by making it openly available regardless of where you choose to publish.. Items in OPUS are enhanced with high quality metadata and seeded to search engines such as Google Scholar as well as being linked to your UTS research profile, increasing discoverability and opportunities for citation of your work and collaboration. In addition, works in OPUS are preserved for long-term access and discovery.. The UTS Open Access Policy requires UTS research outputs to be openly available via OPUS. Depositing your work in OPUS also assists you in complying with ARC, NHMRC and other funder Open Access policies. Providing Open Access to your research outputs through OPUS not only ensures you comply with these important policies, but increases opportunities ...
The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008 ...
Maspin (mammary serine protease inhibitor) is a non-inhibitory serpin that is expressed predominantly in normal mammary epithelial cells but at significantly reduced levels or absent in most breast carcinomas. It has the ability to block the growth, invasiveness, and metastatic potential of breast and lung tumors. This anti-tumor activity is achieved, in part, by the ability of Maspin to inhibit angiogenesis and to preferentially promote apoptosis of tumor cells. Recombinant human Maspin is a 42.2 kDa non-glycosylated protein containing 375 amino acid residues ...
The majority of serpin diseases are due to protein aggregation and are termed serpinopathies.[9][63] Serpins are vulnerable to disease-causing mutations that promote formation of misfolded polymers due to their inherently unstable structures.[63] Well-characterised serpinopathies include α1-antitrypsin deficiency (alpha-1), which may cause familial emphysema and sometimes liver cirrhosis, certain familial forms of thrombosis related to antithrombin deficiency, types 1 and 2 hereditary angioedema (HAE) related to deficiency of C1-inhibitor, and familial encephalopathy with neuroserpin inclusion bodies (FENIB; a rare type of dementia caused by neuroserpin polymerisation).[8][9][68]. Each monomer of the serpin aggregate exists in the inactive, relaxed conformation (with the RCL inserted into the A-sheet). The polymers are therefore hyperstable to temperature and unable to inhibit proteases. Serpinopathies therefore cause pathologies similarly to other proteopathies (e.g. prion diseases) via two ...
Long Noncoding RNA HULC Accelerates Liver Cancer by Inhibiting PTEN via Autophagy Cooperation to miR15a HULC accelerated malignant progression of liver cancer cells in vitro and in vivo. HULC inhibited PTEN through ubiquitin-proteasome system mediated by autophagy-P62. HULC increased the expression of P62 via decreasing mature miR15a. [Mol Cancer] Full Article Inflammasome Activation in Kupffer Cells Confers a Protective Response in Nonalcoholic Steatohepatitis through Pigment Epithelium-Derived Factor Expression Investigators identified pigment epithelium-derived factor, a secreted, moonlighting hepatokine as one hepatoprotective agent in mice with diet-induced nonalcoholic steatohepatitis. [FASEB J] Abstract TRIM52 Up-Regulation in Hepatocellular Carcinoma Cells Promotes Proliferation, Migration and Invasion through the Ubiquitination of PPM1A Tripartite motif containing 52 (TRIM52) was found interacted with Mg2+/Mn2+ dependent 1A (PPM1A) and TRIM52 down-regulation inhibited the ubiquitination ...
BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008 ...
Gene Information This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB) which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jul 2008]. ...
Maspin, a member of the Serpin protease inhibitor family, is overexpressed in poorly differentiated colorectal tumors and more frequently found in tumors with microsatellite instability. Immunohistochemical nuclear Maspin staining is predominantly seen in tumor cells at the invasion front of such cancers, suggesting that this molecule is associated with local tumor cell infiltration and aggressiveness. In a retrospective study, we studied nuclear Maspin expression as a potential prognostic tool in a total of 172 primary stage III colon cancers by immunohistochemistry. Of those 172 patients, 76 were treated by surgery only, and 96 patients received additional adjuvant 5-fluorouracil (5-FU) based chemotherapy. Nuclear Maspin expression was an independent adverse prognostic factor for overall survival in our patient cohort (hazard ratio 2.08; 95% CI, 1.13-3.81; p = 0.018). However, patients with primary tumors expressing Maspin in the nucleus showed a significant treatment benefit from 5-FU ...
TY - JOUR. T1 - Development of specific monoclonal antibodies and a sensitive discriminatory immunoassay for the circulating tumor markers SCCA1 and SCCA2. AU - Çataltepe, Sule. AU - Schick, Charles. AU - Luke, Cliff J.. AU - Pak, Stephen C O. AU - Goldfarb, Daniel. AU - Chen, Philip. AU - Tanasiyevic, Milenko J.. AU - Posner, Marshall R.. AU - Silverman, Gary A.. PY - 2000/5/1. Y1 - 2000/5/1. N2 - The squamous cell carcinoma antigen (SCCA) serves as a serologic marker for advanced squamous cell carcinomas (SCC) of the uterine cervix, lung, esophagus, head and neck and vulva. Elevations in serum levels of SCCA following treatment for SCC correlate with tumor relapse or metastasis. Recent molecular studies show that SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2) that encode for members of the high molecular weight serine proteinase inhibitor (serpin) family. Despite a high degree of similarity in their amino acid sequences, SCCA1 and SCCA2 have distinct biochemical ...
TY - JOUR. T1 - Development of specific monoclonal antibodies and a sensitive discriminatory immunoassay for the circulating tumor markers SCCA1 and SCCA2. AU - Çataltepe, Sule. AU - Schick, Charles. AU - Luke, Cliff J.. AU - Pak, Stephen C.O.. AU - Goldfarb, Daniel. AU - Chen, Philip. AU - Tanasiyevic, Milenko J.. AU - Posner, Marshall R.. AU - Silverman, Gary A.. PY - 2000/5/1. Y1 - 2000/5/1. N2 - The squamous cell carcinoma antigen (SCCA) serves as a serologic marker for advanced squamous cell carcinomas (SCC) of the uterine cervix, lung, esophagus, head and neck and vulva. Elevations in serum levels of SCCA following treatment for SCC correlate with tumor relapse or metastasis. Recent molecular studies show that SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2) that encode for members of the high molecular weight serine proteinase inhibitor (serpin) family. Despite a high degree of similarity in their amino acid sequences, SCCA1 and SCCA2 have distinct biochemical ...
Since 1987 her research has focused on the serpin family of SERine PRoteinase INhibitors, which play important regulatory roles in vivo. They now recognise that the physicochemical properties of the serpin structure that confer protease inhibitor activity also render these proteins sensitive to post-translational modifications (i.e. oxidation, nitrosylation, inter- or intramolecular interactions and non-specific cleavage). These altered forms of inhibitory serpins are detected in tissues and fluids recovered from inflammatory sites, but the important questions of how they are generated, what their biological activities are, and which of them are directly linked to pathologies and/or may be useful markers for characterisation of disease states, remain to be answered. Her objective is to address this challenge, focusing on the role of native (inhibitory) and post-translationally modified (non-inhibitory) forms of Alpha1-Antitrypsin in obstructive pulmonary disease and Alpha1-antichymotrypsin in ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Adenovirus endopeptidase hydrolyses human squamous cell carcinoma antigens in vitro but not ex vivo.: The serpins SCCA1 and SCCA2 are highly expressed in the ep
Attention, guys! This news might be a little bit surprising, but scientists from worldwide believe that they have uncovered a key reason why obese people have a higher risk of health complications when compared to people who are normal-sized!. This time, the specific protein in our body named Pigment Epithelium-derived Factor (PEDF) is the one to be blamed here, as its the main substance that is secreted by fat cells!. For your information, the PEDF can only be produced by fat cells in people, especially for those who have a problem with being overweight. The most shocking thing is the PEDF sends a signal to all the tissues in the body hence, triggering the development of insulin resistance. This amazing discovery may eventually be known as the main condition that leads to type II diabetes!. Besides that, the raising of PEDF levels is also linked to a number of other complications, such as heart disease and high blood pressure.. It seems like we should keep our eyes in these nasty PEDF ...
Question - What is wrong if baby has low Monocytes Neutrophils and high Lymphocytes ?. Ask a Doctor about when and why Complete blood count is advised, Ask a Pediatrician
SERPINA5 Human Recombinant is a single, non-glycosylated polypeptide chain containing 408 amino acids and having a molecular mass of 45.9kDa.
References for Abcams Recombinant Human PEDF protein (ab86705). Please let us know if you have used this product in your publication
バスピン血中濃度はインスリン抵抗性と相関があり、日本人にはrs77060950によって規定される血中濃度高値群が存在す ...
Oh where to begin. I love love love what you did forming the spirals and loops with the headpin wires. It makes me see headpins in a whole new way, as more than just dangles on a basic wrap. (Of course Id be too afraid to try something like that for fear of ruining a headpin.) Then of course your earrings are all so stunning. The way you combined the art beads for the necklace focal is fabulous and adding the seed beads to the chain makes the while thing magical. And last but not least, the glaze on the headpins you threw in the kiln is delicious. ReplyDelete ...
Oh where to begin. I love love love what you did forming the spirals and loops with the headpin wires. It makes me see headpins in a whole new way, as more than just dangles on a basic wrap. (Of course Id be too afraid to try something like that for fear of ruining a headpin.) Then of course your earrings are all so stunning. The way you combined the art beads for the necklace focal is fabulous and adding the seed beads to the chain makes the while thing magical. And last but not least, the glaze on the headpins you threw in the kiln is delicious. ReplyDelete ...
abstract = {Serpins compose the largest superfamily of peptidase inhibitors and are well known as regulators of hemostasis and thrombolysis. Studies using model organisms, from plants to vertebrates, now show that serpins and their unique inhibitory mechanism and conformational flexibility are exploited to control proteolysis in molecular pathways associated with cell survival, development, and host defense. In addition, an increasing number of non-inhibitory serpins are emerging as important elements within a diversity of biological systems by serving as chaperones, hormone transporters, or anti-angiogenic factors ...
Protein Z (PZ) binds to PZ-dependent inhibitor (ZPI) and accelerates the inhibition of the coagulation protease, activated factor X (FXa), in the presence of phospholipids and Ca2+. A 2.3A resolution crystal structure of PZ complexed with ZPI shows that ZPI is a typical serine protease inhibitor and that PZ has a serine protease fold with distorted oxyanion hole and S1 pocket. The 2 molecules bind with fully complementary surfaces spanning over 2400A(2) and involving extensive ionic and hydrophobic interactions. ZPI has an unusual shutter region with a negatively charged residue buried within the hydrophobic core of the molecule. This unique Asp(213) is critical in maintaining the balanced metastability required for optimal protease inhibition, especially when PZ is bound, with its replacement with Asn resulting in increased thermal stability, but decreased efficiency of protease inhibition. The structure of ZPI shows negatively and positively charged surfaces on top of the molecule, in keeping with
Objectives- The number of endothelial progenitor cells (EPCs) that can be obtained from adult bone marrow and peripheral blood to treat cardiovascular diseases is limited. The goal was to examine the endothelial potential of Wharton jelly in human umbilical cord (WJC)-derived stem cells and evaluate their potential to affect neointimal formation after vascular injury.. Methods and Results- Mesenchymal cells (MCs) were isolated from WJC and cultured in endothelial growth medium. Differentiation into late outgrowth endothelial cells (WJC-OECs) was demonstrated by incorporation of acetylated low-density lipoprotein and expression of the endothelial-specific markers. Transplantation of these cells into wire-injured femoral arteries in mice led to rapid reendothelialization. At 4 weeks after injury, the neointima/media area ratio was reduced and strong expression of pigment epithelium-derived factor (PEDF) compared to saline-or MC- or cord blood-OEC-treated mice. WJC-OECs-conditioned medium has an ...
Understanding protein expression profiles of apical periodontitis may contribute to the discovery of novel diagnostic or therapeutic molecular targets. Methods Periapical tissue samples (n = 5) of patients with lesions characterized as nonhealing were submitted for proteomic analysis. Two differentially expressed proteins (heat shock protein 27 [HSP27] and serpin family B member 1 [SERPINB1]) were selected for characterization, localization by immunofluorescence, and association with known bio
Rat kallikrein-binding protein is a novel serine-proteinase inhibitor that forms a covalent complex with tissue kallikrein. We have purified rat kallikrein-binding protein and cloned the cDNA and the gene encoding rat kallikrein-binding protein [Chao, Chai, Chen, Xiong, Chao, Woodley-Miller, Wang, Lu and Chao (1990) J. Biol. Chem. 265, 16394-16401; Chai, Ma, Murray, Chao and Chao (1991) J. Biol. Chem. 266, 16029-16036]. In the present study, we have expressed rat kallikrein-binding protein in Escherichia coli with a T7-polymerase/promoter expression system. A high level of expression was detected by an e.l.i.s.a. with an average of 24.2 mg of recombinant rat kallikrein-binding protein per 1 of culture. The recombinant protein appeared as a major protein in a crude extract of Escherichia coli on SDS/PAGE. It showed a molecular mass of 43 kDa and was recognized by polyclonal antibody to the native rat kallikrein-binding protein in Western-blot analysis. The recombinant rat kallikrein-binding ...
TY - JOUR. T1 - Pigment epithelium-derived factor. T2 - Clinical significance in estrogen-dependent tissues and its potential in cancer therapy. AU - Franco-Chuaire, María Liliana. AU - Ramírez-Clavijo, Sandra. AU - Chuaire-Noack, Lilian. N1 - Publisher Copyright: © 2015, Mashhad University of Medical Sciences. All rights reserved. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 2015/9. Y1 - 2015/9. N2 - Pigment epithelium-derived factor (PEDF) is a glycoprotein that belongs to the family of noninhibitory serpins. The broad spectrum of PEDF biological activity is evident when considering its effects in promoting cell survival and proliferation, as well as its antiangiogenic, antitumor, and antimetastatic properties. Although the structural domains of the PEDF gene that mediate such diverse effects and their mechanisms of action have not been completely elucidated, there is a large body of evidence describing their diverse range of activities; this evidence combined with ...
Looking for online definition of serpin B9 in the Medical Dictionary? serpin B9 explanation free. What is serpin B9? Meaning of serpin B9 medical term. What does serpin B9 mean?
Looking for online definition of Renal carcinoma antigen NY-REN-62 in the Medical Dictionary? Renal carcinoma antigen NY-REN-62 explanation free. What is Renal carcinoma antigen NY-REN-62? Meaning of Renal carcinoma antigen NY-REN-62 medical term. What does Renal carcinoma antigen NY-REN-62 mean?
TY - JOUR. T1 - Attachment-oriented endoscopic surgical management for inverted papillomas in the nasal cavity and paranasal sinuses. AU - Makihara, Seiichiro. AU - Kariya, Shin. AU - Naito, Tomoyuki. AU - Uraguchi, Kensuke. AU - Matsumoto, Junya. AU - Noda, Yohei. AU - Okano, Mitsuhiro. AU - Nishizaki, Kazunori. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Objective: The treatment of all forms of sinonasal inverted papilloma (IP) is a complete, wide, local resection. The main surgical purpose is to remove all diseased mucosa and mucoperiosteum, together with a cuff of normal-looking mucosa at the attachment site, followed by drilling and/or coagulation. Our aim is to present our experiences in endoscopic surgical management of IP by using attachment-oriented excision. Methods: We present 20 cases of sinonasal IP. The data collected includes the histopathological diagnosis, staging, extension of the tumor, tumor attachment site, approach to surgery, serum squamous cell carcinoma antigen (SCCA) level, and ...
Leukocyte elastase inhibitor (LEI) also known as serpin B1 is a protein that in humans is encoded by the SERPINB1 gene. It is a member of the clade B serpins or ov-serpins (ovalbumin related serpins) founded by ovalbumin. MNEI (monocyte/neutrophil elastase inhibitor) is the mouse orthologue of human SerpinB1. SerpinB1 is a cytoplasmic serine protease inhibitor of polymorphonuclear neutrophils. Among other serine proteases, it specifically inhibits neutrophil elastase, PR3 and cathepsin G, all found in neutrophil granules, by a suicide inhibition mechanism. SerpinB1 was found to reduce tissue damage caused by the mentioned proteases during inflammation and has a role in neutrophil homeostasis in mice. In various infection models (e.g. pneumonia) correlation of SerpinB1 absence and lack of microbial clearance have been shown. Different knockout strains serve as model to investigate the role of SerpinB1 in vivo. Serpin GRCh38: Ensembl release 89: ENSG00000021355 - Ensembl, May 2017 GRCm38: Ensembl ...
Domain combinations containing the Serine protease inhibitors superfamily in Loxodonta africana 76_3. Domain architectures illustrate each occurrence of the Serine protease inhibitors superfamily.
Altogether the inhibitory serpins make up some 10% in molar terms, of the proteins in human plasma. Also present, in plasma, though in smaller concentrations, are other serpins that have lost their inhibitory activity but have taken on other functions vital to life; examples are the vasopressor peptide source angiotensinogen, and the thyroxine and corticosteroid binding globulins, TBG and CBG. The reason for the evolutionary success of the serpins is their possession, uniquely amongst the many families of serine protease inhibitors, of a mobile reactive site loop. It is the ability of this loop to profoundly change its conformation that enables the serpins to bind to their target proteases as a virtually irreversible complex. ...
Hidden Markov models representing the SCOP Kazal-type serine protease inhibitors superfamily.Details for each model include model IDs, number of sequences the model was built from, build date, seed sequence from SCOP and family the seed sequence belongs to.
Stein P.E., Leslie A.G.W., Finch J.T., Carrell R.W.. Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) superfamily. The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of plakalbumin, a proteolytically cleaved form of ovalbumin, as a starting model. The final refined model, including four ovalbumin molecules, 678 water molecules and a single metal ion, has a crystallographic R-factor of 17.4% for all reflections between 6.0 and 1.95 A resolution. The root-mean-square deviation from ideal values in bond lengths is 0.02 A and in bond angles is 2.9 degrees. This is the first crystal structure of a member of the serpin family in an uncleaved form. Surprisingly, the peptide that is homologous to the reactive centre of inhibitory serpins adopts an alpha-helical conformation. The implications for the mechanism of inhibition of the inhibitory members of the family is ...
Fingerprint Dive into the research topics of Antiviral Cytokines Induce Hepatic Expression of the Granzyme B Inhibitors, Proteinase Inhibitor 9 and Serine Proteinase Inhibitor 6. Together they form a unique fingerprint. ...
abstract = {BACKGROUND: The Drosophila melanogaster genome contains 29 serpin genes, 12 as single transcripts and 17 within 6 gene clusters. Many of these serpins have a conserved hinge motif characteristic of active proteinase inhibitors. However, a substantial proportion (42%) lacks this motif and represents non-inhibitory serpin-fold proteins of unknown function. Currently, it is not known whether orthologous, inhibitory serpin genes retain the same target proteinase specificity within the Drosophilid lineage, nor whether they give rise to non-inhibitory serpin-fold proteins or other, more diverged, proteins. RESULTS: We collated 188 orthologues to the D. melanogaster serpins from the other 11 Drosophilid genomes and used synteny to find further family members, raising the total to 226, or 71% of the number of orthologues expected assuming complete conservation across all 12 Drosophilid species. In general the sequence constraints on the serpin-fold itself are loose. The critical Reactive ...
Looking for a Serpins Hotel? Stay at Casa Vale do Linteiro from /night, Casa Gaia from $45/night, Serpins Camping from $23/night and more. Compare prices of hotels in Serpins on KAYAK now.
The molecular changes associated with the transition of melanoma cells from radial growth phase (RGP) to vertical growth phase (VGP) and the metastatic phenotype are not very well defined. However, some of the genes involved in this process and their transcriptional regulation are beginning to be elucidated. For example, the switch from RGP to VGP and the metastatic phenotype is associated with loss of the AP-2α transcription factor. AP-2α regulates the expression of c-KIT, MMP-2, VEGF, and the adhesion molecule MCAM/MUC18. Recently, we reported that AP-2α also regulates two G-protein coupled receptors (GPCRs) PAR-1 and PAFR. In turn, the thrombin receptor, PAR-1, regulates the expression of the gap junction protein Connexin-43 and the tumor suppressor gene Maspin. Activation of PAR-1 also leads to overexpression and secretion of proangiogenic factors such as IL-8, uPA, VEGF, PDGF, as well certain integrins. PAR-1 also cooperates with PAFR to regulate the expression of the MCAM/MUC18 via ...
Pigment epithelium-derived factor (PEDF) is an anti-angiogenic, immunomodulatory, and neurotrophic serine protease inhibitor protein. PEDF is evolving as a novel metabolic regulatory protein that plays a causal role in insulin resistance. Insulin resistance is the central pathogenesis of metabolic disorders such as obesity, type 2 diabetes mellitus, polycystic ovarian disease, and metabolic syndrome, and PEDF is associated with them. The current evidence suggests that PEDF administration to animals induces insulin resistance, whereas neutralisation improves insulin sensitivity. Inflammation, lipolytic free fatty acid mobilisation, and mitochondrial dysfunction are the proposed mechanism of PEDF-mediated insulin resistance. This review summarises the probable mechanisms adopted by PEDF to induce insulin resistance, and identifies PEDF as a potential therapeutic target in ameliorating insulin resistance.. ...
TY - JOUR. T1 - Accelerated evolution in the reactive centre regions of serine protease inhibitors. AU - Hill, R E. AU - Hastie, N D. PY - 1987/3/5. Y1 - 1987/3/5. N2 - The serine protease inhibitors (serpins) are a family of proteins that function to control the action of serine proteases in many diverse physiological processes. The functional region or reactive centre of these inhibitors is near the C-terminal end and is an exposed site that acts as a bait for the appropriate serine protease to recognize and covalently bind. The specificity of the inhibitor is determined, at least in part, by a single amino acid that resides in this region at the P1 position. We show here that following a gene duplication event the reactive centres of three related rodent protease inhibitors have diverged from each other at unprecedented rates. This has resulted in proteins with different predicted specificities and we postulate that these changes were fixed by positive darwinian selection and that the most ...
Looking for serine proteinase inhibitor? Find out information about serine proteinase inhibitor. A substance bonded, taped, or dip-dried onto a solid propellant to restrict the burning surface and to give direction to the burning process. Explanation of serine proteinase inhibitor
Improved survival in patients with a peripheral squamous cell carcinoma has been linked to low blood levels of squamous cell carcinoma antigen or absence of tumor invasion into the space between the lungs and chest wall or into blood vessels.
Osteogenesis imperfecta (OI), or brittle bone disease, is a heritable disorder of connective tissue characterized by bone fragility and low bone mass. OI is clinically and genetically heterogeneous with severity varying from mild to perinatal lethal. Mutations in the COL1A1 and COL1A2 genes cause approximately 90% of OI cases. COL1A1 and COL1A2 encode the alpha 1 and alpha 2 procollagen chains of type I collagen. Mutations in these genes cause autosomal dominant OI; OI types I (MIM 166200), II (MIM 166210), III (MIM 259420) and IV (MIM 166220). Mutations in the IFITM5 can also cause autosomal dominant OI, OI type V (MIM 610967). IFITM5 encodes interferon-induced transmembrane protein 5, also known as bone restricted ifitm-like protein, a molecule of unknown function. Mutations in twelve genes have been linked to autosomal recessive OI or autosomal recessive OI-like disorders. One of these genes, SERPINF1, encodes pigment epithelium-derived factor (PEDF), a strong inhibitor of angiogenesis. PEDF ...
Serpins (serine protease inhibitors) comprise a superfamily of more than forty proteins which are implicated in various regulatory cascades in vivo such as blood coagulation, fibrinolysis, complement activation, matrix proteolysis, and inflammation. Since these 45 kD proteins undergo a massive beta-sheet rearrangement during the course of their biological function, serpins are intriguing from the perspectives of protein folding as well as medical relevance. Antichymotrypsin has been engineered to be an inhibitor of human neutrophil elastase in vitro, and the engineered serpin exhibits antiinflammatory properties in vivo. We have determined the structure of this serpin, and current work focuses on a structure-assisted approach toward engineering the specificity and stability of the active serpin. Additionally, we will determine the structure of the serpin in its complex with a target serine protease, and we will also determine the structures of other members of the serpin superfamily, e.g., those ...
CHIMERIZATION AND CHARACTERIZATION OF A MONOCLONAL ANTIBODY WITH POTENT NEUTRALIZING ACTIVITY ACROSS MULTIPLE... | METHOD FOR DETERMINING DELETIONS IN HBV PRE-S2 REGION | CELL CULTURE MEDIUM | MODIFIED PIGMENT EPITHELIUM-DERIVED FACTOR (PEDF) PEPTIDES AND USES THEREOF FOR TREATING NEOVASCULAR DISEASES,... | EGFR and C-Met Fibronectin Type III Domain Binding Molecules |
PMID 20132989] Phenotype and Genotype Characteristics of Age-related Macular Degeneration in a Japanese Population [PMID 20678803] Complement Factor H and High-Temperature Requirement A-1 Genotypes and Treatment Response of Age-related Macular Degeneration [PMID 21174599] Pigment Epithelium-Derived Factor Gene Polymorphisms in Exudative Age-Related Degeneration in a Chinese Cohort ...
The Drosophila melanogaster Serpin 42 Da gene (previously Serpin 4) encodes a serine protease inhibitor that is capable of remarkable functional diversity through the alternative splicing of four different reactive centre loop exons. Eight protein isoforms of Serpin 42 Da have been identified to date, targeting the protease inhibitor to both different proteases and cellular locations. Biochemical and genetic studies suggest that Serpin 42 Da inhibits target proteases through the classical serpin suicide inhibition mechanism, however the crystal structure of a representative Serpin 42 Da isoform remains to be determined. We report two high-resolution crystal structures of Serpin 42 Da representing the A/B isoforms in the cleaved conformation, belonging to two different space-groups and diffracting to 1.7 Å and 1.8 Å. Structural analysis reveals the archetypal serpin fold, with the major elements of secondary structure displaying significant homology to the vertebrate serpin, neuroserpin. Key residues
Extracellular serine protease cascades have evolved in vertebrates and invertebrates to mediate rapid, local reactions to physiological or pathological cues. The serine protease cascade that triggers the Toll signaling pathway in Drosophila embryogenesis shares several organizational characteristics with those involved in mammalian complement and blood clotting. One of the hallmarks of such cascades is their regulation by serine protease inhibitors (serpins). Serpins act as suicide substrates and are cleaved by their target protease, forming an essentially irreversible 1:1 complex. The biological importance of serpins is highlighted by serpin dysfunction diseases, such as thrombosis caused by a deficiency in antithrombin. Here, we describe how a serpin controls the serine protease cascade, leading to Toll pathway activation. Female flies deficient in Serpin-27A produce embryos that lack dorsal-ventral polarity and show uniform high levels of Toll signaling. Since this serpin has been recently shown to
Recombinant Mouse SERPINF1 Protein best suppliers; Recombinant Mouse SERPINF1 Protein best sources; Recombinant Mouse SERPINF1 Protein best vendors; Recombinant Mouse SERPINF1 Protein protocol; Recombinant Mouse SERPINF1 Protein citations; Recombinant Mouse SERPINF1 Protein publications; Recombinant Mouse SERPINF1 Protein papers - Labshake
To our knowledge, maspin is the only serpin that sensitizes apoptosis, whereas all of the other serpins thus far implicated in apoptosis regulation appear to be antiapoptotic (17 , 32 , 33) . Thus, the existing literature offers little insight into the possible molecular mode of maspin action. The goal of the present study was to identify the specific target molecule(s), the modification of which by maspin sensitizes prostate and breast tumor cells toward potential cancer chemotherapeutic agents. By using multiple maspin-transfected cell lines in vitro, we obtained cellular, molecular, and biochemical evidence that supports a key role for Bax in maspin-mediated apoptosis sensitization. Although we may not have exhausted our search because of the ever-growing number of apoptosis regulators, our data seem sufficient to support our new hypothesis that the specific up-regulation of Bax, without changing Bcl-2, Bcl-xl, and Bak expression in maspin-transfected cells, may tip the balance of pro- versus ...
Purified Recombinant Rat Serpinf1 protein, His-tagged from Creative Biomart. Recombinant Rat Serpinf1 protein, His-tagged can be used for research.
Serpina where to buy serpina himalaya forum serpina hotel 3 serpina for anxiety serpina3f anna serpina serpina ne demek serpina music mp3
Abstract C1 inhibitor (C1INH) is a serpin that regulates both complement and contact (kallikrein-kinin) system activation. It consists of a serpin domain that is highly homologous ..
CrmA inhibits its target proteases via a pseudosubstrate mechanism of action. The interaction between the protease and a cleavage site within the reactive center loop (RCL) of CrmA is proposed to provoke a rapid and dramatic conformational change that converts this external loop to a strand within a β-sheet. Stable complexes have been detected between CrmA and its target caspases [19,29,30], consistent with a model in which the enzymes catalytic dyad becomes distorted, leading to the thioacyl enzyme intermediate being trapped so the cleavage reaction is not completed. Caspase-1 and -8 heterotetramers were observed to dissociate into separate subunits during this process, with the larger subunit remaining covalently linked to CrmA [30]. The only experimentally derived structures of Spi-2 family members were of CrmA cleaved by subtilisin [16] or an unidentified bacterial protease [31]. These enzymes cleaved CrmA at sites distinct from, but near to, the caspase scissile bond that links residues ...
Then, two days ago I was trying to make this gorgeous necklace by gluing a bead cap/bail on the end of a smoky quartz tumbled rock that we tumbled and polished ourselves...It was going to be SO beautiful. Well, the first step requires gluing in a headpin to make a loop for the chain to go through so you can make a necklace. (Think of a very thin long straight pin.) So I put the glue in there, put the headpin in, and all of the sudden, Ive got 3 fingers stuck together. And I mean STUCK ...
Gentaur molecular products has all kinds of products like :search , Prospecbio \ Mouse Anti Human Vaspin Vaspin \ ant-366 for more molecular products just contact us
, Neuroserpin Recombinant Protein (Active), GTX48159-PRO, Applications: ELISA, WB, Functional Assay; ELISA, Western Blot (WB), Functional Assay; CrossReactivity: Human
Buy our Recombinant Human Neuroserpin protein. Ab63224 is a full length protein produced in Escherichia coli and has been validated in WB, FuncS, SDS-PAGE…
Alias: 53BP2, Apoptosis-stimulating of p53 protein 2, ASPP2, Bbp, BBP, Bcl2-binding protein, p53-binding protein 2, p53BP2, P53BP2, PPP1R13A, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2 ...
SLC6A20, SIDT1, SIAH2, SGOL1, SGEF, SFMBT1, SETMAR, SETD5, SETD2, SERPINI2, SERPINI1, SENP2, SEMA3F, SEMA3B, SELT, SEC62, Sec61 alpha 1, SEC22A, SEC13, SATB1
High-quality Serpin E2 proteins from ACROBiosystems. Various species and tags of Serpin E2 proteins. Minimal Batch-to-Batch Variation. Bulks in stock.
Mouse polyclonal antibody raised against a full-length human SERPINB2 protein. SERPINB2 (NP_002566.1, 1 a.a. ~ 415 a.a) full-length human protein. (H00005055-B01P) - Products - Abnova
pep: chromosome:VEGA66:13:33661405:33671581:1 gene:OTTMUSG00000000712 transcript:OTTMUST00000001345 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Serpinb6d description:novel serine (or cysteine) proteinase inhibitor, clade B (Serpinb) member ...
Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 2 is a protein that in humans is encoded by ... Extracellular predictions of SERPINs and common domain clades show that ER localisation of SERPINA2 are most likely be more, ... The model was created using swissmodel in EXPASY, and has shown that SERPINA2 preserves a SERPIN reactive centre loop which is ... "Entrez Gene: Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 2". Retrieved 2017-09-21. Hofker ...
CS1 maint: discouraged parameter (link) Meekins, David A.; Kanost, Michael R.; Michel, Kristin (2017). "Serpins in arthropod ... studying lipophorin and serpins in insect hemolymph. In 1991, Kanost became Assistant Professor of Biochemistry at Kansas State ...
serpins:. *alpha-1 (Alpha 1-antichymotrypsin, Alpha 1-antitrypsin). *alpha-2 (Alpha 2-antiplasmin) ...
Like other serpins, PAI-2 has three beta sheets (A, B, C) and nine alpha helices (hA-hI).[6][7] The structure of PAI-2 mutants ... Due to its position on chromosome 18 close to the bcl-2 protooncogene and several other serpins, PAI-2's role in apoptosis has ... Plasminogen activator inhibitor-2 (placental PAI, SerpinB2, PAI-2), a serine protease inhibitor of the serpin superfamily, is a ... PAI-2 uses a suicide inhibition mechanism (a common mechanism for serpins) to irreversibly inactivate tPA and urokinase.[6] ...
serpins:. *alpha-1 (Alpha 1-antichymotrypsin, Alpha 1-antitrypsin). *alpha-2 (Alpha 2-antiplasmin) ...
serpins:. *alpha-1 (Alpha 1-antichymotrypsin, Alpha 1-antitrypsin). *alpha-2 (Alpha 2-antiplasmin) ...
... (PCI) is serine protease inhibitor of serpin type that is found in most tissues and fluids, including blood ... It is a 52kD glycoprotein and belongs to serine protease inhibitor ( Serpin) super family of protein. In the beginning protein ... Protein C inhibitor (PCI, SERPINA5) is a serine protease inhibitor (serpin) that limits the activity of protein C (an ... Hayashi T, Suzuki K (October 1993). "Gene organization of human protein C inhibitor, a member of SERPIN family proteins encoded ...
Kaiserman D, Bird PI (April 2010). "Control of granzymes by serpins". Cell Death and Differentiation. 17 (4): 586-95. doi: ...
Cite journal requires ,journal= (help) Crenn, K; Serpin, D; Lepleux, C; Overmann, J; Jeanthon, C (November 2016). "Silicimonas ...
Bjork, I; Olson, JE (1997). Antithrombin, A bloody important serpin (in Chemistry and Biology of Serpins). Plenum Press. pp. 17 ... Antithrombin is a serpin (serine protease inhibitor) and is thus similar in structure to most other plasma protease inhibitors ... The P' region of antithrombin is unusually long relative to the P' region of other serpins and in un-activated or heparin ... O'Reilly MS, Pirie-Shepherd S, Lane WS, Folkman J (1999). "Antiangiogenic activity of the cleaved conformation of the serpin ...
Ye S, Goldsmith EJ (2002). "Serpins and other covalent protease inhibitors". Curr. Opin. Struct. Biol. 11 (6): 740-5. doi: ...
Hence the serpin functions as a suicide inhibitor of the protease. This transition does not occur in serpins that lack ... Serpins have a complex structure, a key component of which is the reactive site loop, RSL. Inhibitory serpins transition ... Zou Z, Anisowicz A, Hendrix MJ, Thor A, Neveu M, Sheng S, Rafidi K, Seftor E, Sager R (1994). "Maspin, a serpin with tumor- ... A tumor suppressing serpin". Adv. Exp. Med. Biol. 425: 77-88. doi:10.1007/978-1-4615-5391-5_8. PMID 9433491. Sheng S (2006). " ...
A1AT is a 52-kDa serpin and, in medicine, it is considered the most prominent serpin; the terms α1-antitrypsin and protease ... Most serpins inactivate enzymes by binding to them covalently. These enzymes are released locally in relatively low ... Alpha-1 antitrypsin or α1-antitrypsin (A1AT, α1AT, A1A, or AAT) is a protein belonging to the serpin superfamily. It is encoded ... An extremely rare form of Pi, termed PiPittsburgh, functions as an antithrombin (a related serpin), due to a mutation ( ...
... displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a ... When heated, ovalbumin undergoes a conformational change from its soluble, serpin structure into an insoluble all-β-sheet ... Gettins PG (December 2002). "Serpin structure, mechanism, and function". Chemical Reviews. 102 (12): 4751-804. doi:10.1021/ ... studies of serpin structure and function (the fact that ovalbumin does not inhibit proteases means that by comparing its ...
... , also known as SERPINH1 is a serpin which serves as a human chaperone protein for collagen. This protein ... "Entrez Gene: SERPINH1 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)". ... Gettins PG (December 2002). "Serpin structure, mechanism, and function". Chemical Reviews. 102 (12): 4751-804. doi:10.1021/ ... is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. HSP47 is ...
SPI-2 belongs in superfamily of the inhibitors of serine proteases (serpins). Serpins are the most broadly distributed family ... In mammals serpins are secreted in plasma where they serve as inhibitors of proteases involved in blood coagulation, ... Kettle S, Blake NW, Law KM, Smith GL (January 1995). "Vaccinia virus serpins B13R (SPI-2) and B22R (SPI-1) encode M(r) 38.5 and ... Vaccinia virus encodes two more serpin - SPI-1 and SPI-3. SPI-1 influences the host range and SPI-3 stops infected cells from ...
Serpin B4 is a protein that in humans is encoded by the SERPINB4 gene. Serpin GRCh38: Ensembl release 89: ENSG00000206073 - ... 2000). "Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous ... 2003). "SCCA2-like serpins mediate genetic predisposition to skin tumors". Cancer Res. 63 (8): 1871-5. PMID 12702576. Pontisso ... "Entrez Gene: SERPINB4 serpin peptidase inhibitor, clade B (ovalbumin), member 4". Barnes RC, Worrall DM (1995). "Identification ...
One group of inhibitors are the Serpins (Serine Protease Inhibitors). Neutrophil elastase has been shown to interact with Alpha ... 2-antiplasmin, which belongs to the Serpin family of proteins. Elastase ENSG00000197561 GRCh38: Ensembl release 89: ...
The C-terminal serpin domain is similar to other serpins, which is the part of C1-inhibitor that provides the inhibitory ... C1-inhibitor is the largest member among the serpin superfamily of proteins. It can be noted that, unlike most family members, ... C1-inhibitor (C1-inh, C1 esterase inhibitor) is a protease inhibitor belonging to the serpin superfamily. Its main function is ... 2006). "An overview of the serpin superfamily". Genome Biology. 7 (5): 216. doi:10.1186/gb-2006-7-5-216. PMC 1779521. PMID ...
Others give negative feedback on the inflammatory response, e.g. serpins. Alpha 2-macroglobulin and coagulation factors affect ...
Panyutich AV, Hiemstra PS, van Wetering S, Ganz T (1995). "Human neutrophil defensin and serpins form complexes and inactivate ...
The role of serin peptidase inhibitors (Serpins) is also being investigated. SerpinA1 was found to be elevated in the ...
In terms of genomics, TBG is a serpin; however, it has no inhibitory function like many other members of this class of proteins ...
Valiente M, Obenauf AC, Jin X, Chen Q, Zhang XH, Lee DJ, Chaft JE, Kris MG, Huse JT, Brogi E, Massagué J (2014). "Serpins ... However, recent studies have noted these cancer cells overproduce anti-PA serpins, which are the usual inhibitors of plasmin, ...
"Serpins Promote Cancer Cell Survival and Vascular Co-Option in Brain Metastasis". Cell. 156 (5): 1002-1016. doi:10.1016/j.cell. ...
Sea squirts express several serpin genes, suggesting that the PEDF gene may have arisen from another serpin family member after ... "Pigment epithelium-derived factor behaves like a noninhibitory serpin. Neurotrophic activity does not require the serpin ... "Entrez Gene: SERPINF1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1". ... Filleur S, Nelius T, de Riese W, Kennedy RC (Apr 2009). "Characterization of PEDF: a multi-functional serpin family protein". ...
Serpin A9 is a member of the serpin family of serine protease inhibitors. The expression of SERPINA9 is restricted to germinal ... Serpin A9 also known as centerin or GCET1 is a protein that in humans is encoded by the SERPINA9 gene located on chromosome ... Paterson MA, Hosking PS, Coughlin PB (July 2008). "Expression of the serpin centerin defines a germinal center phenotype in B- ... 2000). "Identification of centerin: a novel human germinal center B cell-restricted serpin". Eur. J. Immunol. 30 (10): 3039-48 ...
Turato, C.; Pontisso, P. (2015). "SERPINB3 (serpin peptidase inhibitor, clade B (ovalbumin), member 3)". Atlas of Genetics and ...
Serpins are well-characterized inhibitors of the chymotrypsin family serine proteinases. We have investigated the interaction ... When inhibition occurs, the rate of formation and stability of the complexes are similar to those formed between serpins and ... The ratio decreases as temperature is elevated in the range 0-45 degrees C, indicating that the serpins are more efficient ... Interaction of subtilisins with serpins.. Komiyama T1, Grøn H, Pemberton PA, Salvesen GS. ...
Serpins Camping from $23/night and more. Compare prices of hotels in Serpins on KAYAK now. ... Looking for a Serpins Hotel? Stay at Casa Vale do Linteiro from /night, Casa Gaia from $45/night, ... Serpins Hotel Deals. Search for hotels in Serpins. Find cheap hotel rooms in Serpins. KAYAK searches hundreds of travel sites ... Serpins hotels. Search hundreds of travel sites at once for hotels in Serpins. ...
2 serpins), 42D (5 serpins), 43A (4 serpins), 77B (3 serpins) and 88E (2 serpins). Studies on Drosophila serpins reveal that ... The term uterine serpin refers to members of the serpin A clade that are encoded by the SERPINA14 gene. Uterine serpins are ... Amino acid sequence analysis has placed 14 of these serpins in serpin clade Q and three in serpin clade K with the remaining ... Examples of cross-class inhibitory serpins include serpin B4 a squamous cell carcinoma antigen 1 (SCCA-1) and the avian serpin ...
In other analyses, uterine serpins have been considered as a separate clade in the serpin superfamily. The uterine serpins are ... Ovine uterine serpin also binds to activin, IgM and IgA. Another possible role for uterine serpins is in the inhibition of ... Uterine serpins are members of the A clade of the serine protease inhibitor (serpin) superfamily of proteins and are encoded by ... There are two lines of evidence to indicate that uterine serpins do not function as protease inhibitors. Uterine serpins from ...
Serpin H1 inhibitory domain (IPR033830). Short name: Serpin_H1_domain Overlapping homologous superfamilies *Serpin superfamily ... The serpin does not inhibit serine peptidases, unlike most serpins, but has been shown to inhibit the lysosomal cysteine ...
Serpin synonyms, Serpin pronunciation, Serpin translation, English dictionary definition of Serpin. also in·hib·it·er n. One ... redirected from Serpin). Also found in: Thesaurus, Medical, Legal, Encyclopedia, Wikipedia.. Related to Serpin: Serine protease ... Serpin A1 or 1-antitrypsin is included in the serine peptidase inhibitors (Serpins) family which has a very large distribution ... Serpin - definition of Serpin by The Free Dictionary https://www.thefreedictionary.com/Serpin ...
Term: serpin. ID: PIRSF001630 Mouse Protein Superfamily Annotations. Select one or more mouse PIRSF members to download protein ...
Leukocyte elastase inhibitor (serpin B1) (IPR015557). Short name: Serpin_B1 Overlapping homologous superfamilies *Serpin ... Leukocyte elastase inhibitor is also known as serpin B1 (MEROPS identifier I04.006). Serpins (SERine Proteinase INhibitors) ... Serpin B1 regulates the activity of neutrophil serine proteases such as elastase, cathepsin G and proteinase-3 and may play a ... The serpin MNEI inhibits elastase-like and chymotrypsin-like serine proteases through efficient reactions at two active sites. ...
Recombinant Mouse Serpin A1/alpha-1-Antitrypsin, CF Coagulation, Serine Protease Regulators,biological,biology supply,biology ... Recombinant Mouse Serpin A1/alpha-1-Antitrypsin, CF Protein Family: Coagulation, Serine Protease Regulators. ... Mouse Serpin A1 from R&D Systems, ...
Serpins thus provide stoichiometric, irreversible inhibition, and their dependence on conformational change is exploited fo … ... Serpins use an extraordinary mechanism of protease inhibition that depends on a rapid and marked conformational change and ... Shape-shifting serpins--advantages of a mobile mechanism Trends Biochem Sci. 2006 Aug;31(8):427-35. doi: 10.1016/j.tibs.2006.06 ... Serpins use an extraordinary mechanism of protease inhibition that depends on a rapid and marked conformational change and ...
Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in ... Serpins: A family of serine proteinase inhibitors which are similar in amino acid sequence and mechanism of inhibition, but ... Serpins. Subscribe to New Research on Serpins A family of serine proteinase inhibitors which are similar in amino acid sequence ... Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in ...
It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by ... The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism ... Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition.. Simonovic, M., ... CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host ...
serpin A4. serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4. sserpin family A member 4. ... SERPINA4 serpin family A member 4 [Homo sapiens] SERPINA4 serpin family A member 4 [Homo sapiens]. Gene ID:5267 ... serpin family A member 4provided by HGNC. Primary source. HGNC:HGNC:8948 See related. Ensembl:ENSG00000100665 MIM:147935 Gene ... SERPINA4 serpin family A member 4 [ Homo sapiens (human) ] Gene ID: 5267, updated on 24-Nov-2020 ...
IPR023796, Serpin_dom. IPR000215, Serpin_fam. IPR036186, Serpin_sf. IPR042178, Serpin_sf_1. IPR042185, Serpin_sf_2. ... IPR023796, Serpin_dom. IPR000215, Serpin_fam. IPR036186, Serpin_sf. IPR042178, Serpin_sf_1. IPR042185, Serpin_sf_2. ... Belongs to the serpin family. Ov-serpin subfamily.Curated. Phylogenomic databases. evolutionary genealogy of genes: Non- ... "Inhibitory mechanism of a cross-class serpin, the squamous cell carcinoma antigen 1.". Masumoto K., Sakata Y., Arima K., Nakao ...
As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits ... IPR000240. Serpin_B9/Maspin. IPR023795. Serpin_CS. IPR023796. Serpin_dom. IPR000215. Serpin_fam. IPR036186. Serpin_sf. ... IPR000240. Serpin_B9/Maspin. IPR023795. Serpin_CS. IPR023796. Serpin_dom. IPR000215. Serpin_fam. IPR036186. Serpin_sf. ... Belongs to the serpin family. Ov-serpin subfamily.Curated. Phylogenomic databases. evolutionary genealogy of genes: Non- ...
Compare serpin-1 ELISA Kits from Biomatik from leading suppliers on Biocompare. View specifications, prices, citations, reviews ... serpin-1 ELISA Kits from Biomatik. The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and ... Your search returned 62 serpin-1 ELISA ELISA Kit across 1 supplier. ...
Abraham C.R., Potter H. (1990) The Serpin, α1-Antichymotrypsin, in Brain Aging and Diseases of the Nervous System. In: Festoff ... The Serpin, α1-Antichymotrypsin, in Brain Aging and Diseases of the Nervous System. ... Serine Proteases and Their Serpin Inhibitors in the Nervous System pp 321-327 , Cite as ... B.W., Hantaï D. (eds) Serine Proteases and Their Serpin Inhibitors in the Nervous System. NATO ASI Series (Series A: Life ...
... a Myxomavirus-Derived Immune Modulating Serpin: Structural Design of Serpin Reactive Center Loop Peptides with Improved ... Serpin Inhibition Mechanism: A Delicate Balance between Native Metastable State and Polymerization. Mohammad Sazzad Khan,1 ... Maja Cohen, and Robert Fluhr, " Noncanonical interactions between serpin and β-amylase in barley grain improve β-amylase ... Swapan Roy, and Matthew Kuruc, "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors," Functional ...
Active Recombinant human Serpin A5 protein (Active) is an Escherichia coli Full length protein 20 to 406 aa range, , 90% purity ... Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5. *Serpin peptidase inhibitor clade A member 5 ... Recombinant human Serpin A5 protein (Active). See all Serpin A5 proteins and peptides. ...
found that serpin knockdown in metastatic cell lines removed the serpin protection mechanism in mice brain slice assays. The ... Serpins help breast and lung cancer cells metastasize to the brain by annulling stromal defenses and co-opting capillaries. ... Serpins help breast and lung cancer cells metastasize to the brain by annulling stromal defenses and co-opting capillaries. ... M. Valiente et al., Serpins promote cancer cell survival and vascular co-option in brain metastasis. Cell 156, 1002-1016 (2014 ...
Browse our Serpin C1/Antithrombin-III all backed by our Guarantee+. ... Serpin C1/Antithrombin-III available through Novus Biologicals. ...
Serpin I1 explanation free. What is Serpin I1? Meaning of Serpin I1 medical term. What does Serpin I1 mean? ... Looking for online definition of Serpin I1 in the Medical Dictionary? ... redirected from Serpin I1) SERPINI1. A gene on chromosome 3q26.1 that encodes a member of the serpin superfamily of serine ... serpin peptidase inhibitor, clade D (heparin cofactor), member 1. *serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 ...
Crystal Structure of a Thermophilic Serpin, Tengpin, in the Native State. 2pef. Crystal Structure of a Thermophilic Serpin, ... Serpins (SERine Proteinase INhibitors) belong to MEROPS inhibitor family I4, clan ID. Most serpin family members are indeed ... THE STRUCTURE OF ACTIVE SERPIN K FROM MANDUCA SEXTA AND A MODEL FOR SERPIN-PROTEASE COMPLEX FORMATION. ... The serpins: evolution and adaptation in a family of protease inhibitors.. *Cold Spring Harb Symp Quant Biol. 1987; 52: 527-35 ...
View our 5 Serpin A10/ZPI products for your research including Serpin A10/ZPI ELISAs, Luminex Assays, Primary Antibodies, ... Serpin A10/ZPI: Products. The human serpin superfamily consists of at least 35 members that target not only serine proteases, ... As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, ... but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major ...
Browse our Serpin G1/C1 Inhibitor Protein catalog backed by our Guarantee+. ... Serpin G1/C1 Inhibitor Proteins available through Novus Biologicals. ... Serpin G1/C1 Inhibitor Proteins. We offer Serpin G1/C1 Inhibitor Peptides and Serpin G1/C1 Inhibitor Proteins for use in common ... Our Serpin G1/C1 Inhibitor Peptides and Serpin G1/C1 Inhibitor Proteins can be used in a variety of model species: Human. Use ...
... J Biol Chem. 1997 Mar 21;272(12):7797-800. doi ...
The worlds first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.
Serine Proteinase Inhibitors (serpins). Class Summary. Depending on the particular brand, these concentrates are used for ... C1 inhibitor is a normal constituent of human blood and is one of the serine proteinase inhibitors (serpins). It regulates ...
Serpin A1 Human ELISA Kit from Invitrogen (96 Tests). Quantitate human alpha-1 Antitrypsin in plasma, serum and supernatant. ... The assay will exclusively recognize both natural and recombinant Hu Serpin A1.. Principle of the method. The Human Serpin A1 ... The Human Serpin A1 ELISA quantitates Hu Serpin A1 in human serum, plasma, or cell culture medium. ... serpin peptidase inhibitor clade A member 1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), Short ...
How serpins change their fold for better and for worse Robin W. Carrell ; Robin W. Carrell 1 ... Using serpins cysteine protease cross-specificity to possibly trap SARS-CoV-2 Mpro with reactive center loop chimera Clin Sci ( ... The serpins differ from the many other families of serine protease inhibitors in that they undergo a profound change in ... Inhibitory serpins. New insights into their folding, polymerization, regulation and clearance Biochem J (July,2016) ...
  • Serpin polymerisation not only reduces the amount of active inhibitor, but also leads to accumulation of the polymers, causing cell death and organ failure. (wikipedia.org)
  • For example, extracellular serpins regulate the proteolytic cascades central to blood clotting (antithrombin), the inflammatory and immune responses (antitrypsin, antichymotrypsin, and C1-inhibitor) and tissue remodelling (PAI-1). (wikipedia.org)
  • Uterine serpins are members of the A clade of the serine protease inhibitor (serpin) superfamily of proteins and are encoded by the SERPINA14 gene. (wikipedia.org)
  • Global Markets Direct's, 'Plasminogen Activator Inhibitor-1 (Endothelial Plasminogen Activator Inhibitor or Serpin E1 or PAI-1) - Pipeline Review, H1 2016', provides in depth analysis on Plasminogen Activator Inhibitor-1 (Endothelial Plasminogen Activator Inhibitor or Serpin E1 or PAI-1) targeted pipeline therapeutics. (thefreedictionary.com)
  • Leukocyte elastase inhibitor is also known as serpin B1 (MEROPS identifier I04.006). (ebi.ac.uk)
  • Serpins (SERine Proteinase INhibitors) belong to MEROPS inhibitor family I4 (clan ID) [ PMID: 14705960 ]. (ebi.ac.uk)
  • Characterization of four murine homologs of the human ov-serpin monocyte neutrophil elastase inhibitor MNEI (SERPINB1). (ebi.ac.uk)
  • We offer Serpin G1/C1 Inhibitor Peptides and Serpin G1/C1 Inhibitor Proteins for use in common research applications: Blocking/Neutralizing, Control, ELISA, Protein Array, SDS-Page, Western Blot. (novusbio.com)
  • Each Serpin G1/C1 Inhibitor Peptide and Serpin G1/C1 Inhibitor Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Our Serpin G1/C1 Inhibitor Peptides and Serpin G1/C1 Inhibitor Proteins can be used in a variety of model species: Human. (novusbio.com)
  • Plasminogen Activator Inhibitor-2 (Serpine2) which inhibits thrombin, plasmin and plasminogen activators is a part of the Serpin superfamily of the serine protease inhibitors. (prospecbio.com)
  • Serpin G1 is a serine protease inhibitor protein. (gbiosciences.com)
  • Similar to ovalbumin and OVAY, OVAX belongs to the ovalbumin serine protease inhibitor family (ov-serpin). (sigmaaldrich.com)
  • The SERPINB8 gene encodes a protein belonging to the high molecular weight serine proteinase inhibitor (SERPIN) superfamily. (cags.org.ae)
  • We have identified a member of the serine protease inhibitor (serpin) family which is highly induced in macrophages during bacillus Calmette-Guérin infection. (jimmunol.org)
  • On www.antibodies-online.com are 150 serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 7 (SERPINA7) Antibodies from 18 different suppliers available. (antibodies-online.com)
  • Additionally we are shipping serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 7 Kits (56) and serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 7 Proteins (33) and many more products for this protein. (antibodies-online.com)
  • A total of 245 serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 7 products are currently listed. (antibodies-online.com)
  • Serpin-mediated regulation may occur either at the level of plasmin, mainly by α2-antiplasmin (α2-AP) or at the level of the PAs, mainly by plasminogen activator inhibitor -1 (PAI-1). (eurekaselect.com)
  • Other serpins may also be involved including plasminogen activator inhibitor -2 and -3 (PAI-2 and PAI-3), protease nexin-1 (PN-1), C1-inhibitor (C1-INH), placental thrombin inhibitor (PTI), neuroserpin, and yukopin. (eurekaselect.com)
  • Among these genes, HEADPIN, which was isolated at chromosome 18q21.3, belongs to the member of serine proteinase inhibitor family (serpin). (nii.ac.jp)
  • Members of the family are diversely present in eukaryotes, plants and viruses, and are evident in everyday life from the white of the breakfast egg - the non-inhibitory serpin ovalbumin, to the foam protein of the evening beer - the barley Z protease inhibitor. (cam.ac.uk)
  • Members of the serpin (serine proteinase inhibitor) superfamily have been identified in higher multicellular eukaryotes (plants and animals) and viruses but not in bacteria, archaea, or fungi. (edu.au)
  • The protein encoded by this gene is a member of the serpin (serine proteinase inhibitor) superfamily, and ovalbumin(ov)-serpin subfamily. (creative-biogene.com)
  • Defects in Serpin E1 / PN1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma. (acrobiosystems.com)
  • Plasminogen activator inhibitor-2 (placental PAI, SerpinB2, PAI-2), a serine protease inhibitor of the serpin superfamily, is a coagulation factor that inactivates tPA and urokinase . (wikipedia.org)
  • 2014). Repression of liver colorectal metastasis by the serpin Spn4A a naturally occurring inhibitor of the constitutive secretory proprotein convertases. (uni-bielefeld.de)
  • For instance, viral serpin crmA5 and individual serpin PI96 can inhibit members of the caspase family, and serpin squamous cell carcinoma antigen 17,8 can inhibit cathepsins K, L, and S. Additionally, the plasma serpin inhibitor of coagulation protease, antithrombin, has been shown to inactivate papain9 and Arg-gingipain (bacterial cysteine protease)10. (healthandwellnesssource.org)
  • This Human Serpin G1/C1 Inhibitor DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Serpin G1/C1 Inhibitor. (fishersci.com)
  • Serpins are a superfamily of proteins with similar structures that were first identified for their protease inhibition activity and are found in all kingdoms of life. (wikipedia.org)
  • Protease inhibition by serpins controls an array of biological processes, including coagulation and inflammation, and consequently these proteins are the target of medical research. (wikipedia.org)
  • Although most serpins control proteolytic cascades, some proteins with a serpin structure are not enzyme inhibitors, but instead perform diverse functions such as storage (as in egg white-ovalbumin), transport as in hormone carriage proteins (thyroxine-binding globulin, cortisol-binding globulin) and molecular chaperoning (HSP47). (wikipedia.org)
  • Around the same time, the first structures were solved for serpin proteins (first in the relaxed, and later in the stressed conformation). (wikipedia.org)
  • Over 1000 serpins have now been identified, including 36 human proteins, as well as molecules in all kingdoms of life-animals, plants, fungi, bacteria, and archaea-and some viruses. (wikipedia.org)
  • Termed uterine milk proteins, their identification as members of the serpin superfamily was based on sequencing of the cDNA for the sheep gene. (wikipedia.org)
  • There are 6797 SERPIN domains in 6727 proteins in SMART's nrdb database. (embl-heidelberg.de)
  • Taxonomic distribution of proteins containing SERPIN domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with SERPIN domain is also avaliable . (embl-heidelberg.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing SERPIN domain in the selected taxonomic class. (embl-heidelberg.de)
  • The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. (rndsystems.com)
  • It is the largest member among the serpin class of proteins. (gbiosciences.com)
  • The N-terminal domain is not essential for Serpin G1 to inhibit proteinases and has no similarity to other proteins. (gbiosciences.com)
  • Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. (iberlibro.com)
  • The cowpox virus-encoded anti-apoptotic protein cytokine response modifier A (CrmA) is a member of the serpin family that specifically inhibits the cellular proteins caspase 1, caspase 8 and granzyme B. In this study, we have used Flag- and yellow fluorescent protein (YFP)-tagged versions of CrmA to investigate the mechanisms that regulate its subcellular localization. (biochemj.org)
  • We have investigated whether VV genes B13R and B22R, which encode proteins with amino acid similarity to serine protease inhibitors (serpins), are involved in this process. (ox.ac.uk)
  • Recombinant VVs were constructed which express influenza virus proteins HA, NP or NS1 and which lack serpin gene B13R or both B13R and B22R. (ox.ac.uk)
  • The lysis of cells infected with these viruses by influenza virus-specific CD8+ CTL was compared to the lysis of cells infected with viruses expressing both the influenza proteins and the serpin genes. (ox.ac.uk)
  • The serpin superfamily is characterized by proteins that fold into a conserved tertiary structure and exploits a sophisticated and irreversible suicide-mechanism of inhibition. (peerj.com)
  • Several gene clusters have arisen, encoding functionally diverse serpin proteins. (peerj.com)
  • Altogether the inhibitory serpins make up some 10% in molar terms, of the proteins in human plasma. (cam.ac.uk)
  • Despite considerable sequence divergence between the proteins, in four of the 12 sequences the region of the serpin that determines proteinase specificity is highly conserved, indicating that these inhibitors are likely to share a common target. (edu.au)
  • Serpins are a unique breed of proteins due to their enzymatic mechanism. (vcu.edu)
  • Three homologous serpins have been identified in the silk gland (a highly specialized organ that functions to synthesize and store silk proteins) of and its paralogs (and showed similar expression patterns during the silkworm development: expression levels increased from the first to the fifth day, decreased around the seventh day, and disappeared by the wandering stage (Fig. 4A). (healthandwellnesssource.org)
  • To explore the expression pattern of all the three serpin proteins, we performed western blot analysis using a polyclonal antibody against serpin16, an antibody that exhibits cross reactivity with all three serpins as a consequence of sequence similarities (see Supplementary Information). (healthandwellnesssource.org)
  • The serpins (SERine Proteinase INhibitors) are a family of proteins with important physiological roles, including but not limited to the inhibition of chymotrypsin-like serine proteinases. (elsevier.com)
  • Serpins are also an unusual family of proteins in which homologues have native states with different folding topologies. (elsevier.com)
  • This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. (genecards.org)
  • The acronym serpin was originally coined because the first serpins to be identified act on chymotrypsin-like serine proteases (serine protease inhibitors). (wikipedia.org)
  • Most serpins are protease inhibitors, targeting extracellular, chymotrypsin-like serine proteases. (wikipedia.org)
  • Examples of cross-class inhibitory serpins include serpin B4 a squamous cell carcinoma antigen 1 (SCCA-1) and the avian serpin myeloid and erythroid nuclear termination stage-specific protein (MENT), which both inhibit papain-like cysteine proteases. (wikipedia.org)
  • Approximately two-thirds of human serpins perform extracellular roles, inhibiting proteases in the bloodstream in order to modulate their activities. (wikipedia.org)
  • Uterine serpins from the sheep and pig are not inhibitory towards a variety of proteases. (wikipedia.org)
  • Ovine uterine serpin binds pregnancy-associated glycoproteins, which are inactive aspartic proteases secreted in large amounts by the ungulate placenta. (wikipedia.org)
  • Serpin B1 regulates the activity of neutrophil serine proteases such as elastase, cathepsin G and proteinase-3 and may play a regulatory role to limit inflammatory damage due to proteases of cellular origin [ PMID: 11747453 ]. (ebi.ac.uk)
  • The serpin MNEI inhibits elastase-like and chymotrypsin-like serine proteases through efficient reactions at two active sites. (ebi.ac.uk)
  • Serpins form covalent complexes with target proteases. (embl-heidelberg.de)
  • Serpins (serine protease inhibitors) regulate some innate immune responses of insects by inhibiting endogenous proteases. (pubmedcentralcanada.ca)
  • Determining the biological functions of the mosquito serpins will require future work to identify the proteases they inhibit in vivo . (pubmedcentralcanada.ca)
  • Most serpins inhibit serine proteases, although some can inhibit proteases from other classes. (pubmedcentralcanada.ca)
  • These proteases are regulated by serine protease inhibitors (serpins). (eurekaselect.com)
  • The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. (iberlibro.com)
  • Serpins regulate specific active proteases by covalently binding and forming serpin-protease complexes. (k-state.edu)
  • Discovering the serpin-1 isoforms that inhibit specific proteases enhances our understanding of the regulation of proteolytic cascades in M. sexta. (k-state.edu)
  • It is the ability of this loop to profoundly change its conformation that enables the serpins to bind to their target proteases as a virtually irreversible complex. (cam.ac.uk)
  • However, recent research show that many serpins display inhibitory activity against cysteine proteases. (healthandwellnesssource.org)
  • One class of versatile inhibitors is definitely serpins, serine protease inhibitors that act as suicide-like substrates, irreversibly inhibiting their targets, which are primarily serine proteases. (biobeds.info)
  • The conformational-change mechanism confers certain advantages, but it also has drawbacks: serpins are vulnerable to mutations that can result in serpinopathies such as protein misfolding and the formation of inactive long-chain polymers. (wikipedia.org)
  • The pig uterine serpin was first identified because of its association with an iron-binding uterine protein termed uteroferrin. (wikipedia.org)
  • Late in pregnancy, uterine serpin protein can be found in the lumenal epithelium as well, perhaps as glandular epithelial cells move to the uterine lumen. (wikipedia.org)
  • Analysis of mRNA, protein levels, and conditioned media in human cancer cell lines and metastasis tissue revealed a significant up-regulation of neuroserpin and serpins B2, E1, and E2 only in brain metastatic cells and tissue, but not in other cancer cells or in metastases present in other organs. (sciencemag.org)
  • Because the deduced amino acid sequence qualified the protein as a novel member of the serpin family of serine protease inhibitors, we called it neuroserpin. (embl-heidelberg.de)
  • Serpin G1 is an acute phase protein and circulates in blood at levels of around 0.25g/L, whose levels rise 2-fold during inflammation. (gbiosciences.com)
  • Ovalbumin-related protein X is a heparin-binding ov-serpin exhibiting antimicrobial activities. (sigmaaldrich.com)
  • A gene encoding a protein related to the serpin family of protease inhibitors was identified as a candidate tumor suppressor gene that may play a role in human breast cancer. (sciencemag.org)
  • In this screen, we identified serpin 2a (spi2a) as a protein with substantially increased expression during BCG infection in vivo ( 6 , 7 ). (jimmunol.org)
  • Serpins are a protein superfamily with conserved structure that regulate both serine and cysteine protease function in diverse processes including coagulation, extracellular matrix degradation, complement activation, fibrinolysis, and apoptosis ( 8 , 9 ). (jimmunol.org)
  • It was postulated that in the blood-feeding members of the monogenean family Diplozoidae, serpins could play an important role in the prevention of thrombus formation, activation of complement, inflammation in the host, and/or in the endogenous regulation of protein degradation. (muni.cz)
  • Results: In silico analysis showed that the DNA and primary protein structures of serpin from Eudiplozoon nipponicum (EnSerp1) are similar to other members of the serpin superfamily. (muni.cz)
  • The mAb 4B12 epitope was found to encompass residues Glu32, Glu39, and His43 on helix A and Leu306 on helix I. This is not a region typically associated with the serpin mechanism of conformational change, and correspondingly the epitope was present in all tested structural forms of the protein. (biochemj.org)
  • Serpin-1, an abundant plasma protein, has an alternatively spliced ninth exon encoding 12 serpin-1 isoforms that differ in inhibitory selectivity. (k-state.edu)
  • Nine out of the twelve serpin-1 isoforms were identified from control plasma at the protein level using 2D-PAGE. (k-state.edu)
  • Small volumes of serpin-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • The protein encoded by this gene is a member of the ov-serpin family of serine protease inhibitors. (nih.gov)
  • In this study we characterize 12 serpin-like sequences in the genomes of prokaryotic organisms, extending this protein family to all major branches of life. (edu.au)
  • This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse recombinant protein of Serpin C1 (also called antithrombin III). (angioproteomie.com)
  • Meanwhile, the formation of a serpin-protease complex requires that a portion of the RCL inserts into -sheet A of the serpin protein, thereby carrying the covalently bound protease from the top to the bottom of the serpin. (healthandwellnesssource.org)
  • The serpin structures illustrate detailed control of conformation within a single protein. (elsevier.com)
  • SERPINE2 (Serpin Family E Member 2) is a Protein Coding gene. (genecards.org)
  • Serpins are well-characterized inhibitors of the chymotrypsin family serine proteinases. (nih.gov)
  • The partition ratio is caused by the serpins acting as substrates or inhibitors. (nih.gov)
  • The ratio decreases as temperature is elevated in the range 0-45 degrees C, indicating that the serpins are more efficient inhibitors at high temperature. (nih.gov)
  • The name "serpin" was coined based on the most common activity of the superfamily (serine protease inhibitors). (wikipedia.org)
  • Serpins are therefore the largest and most diverse superfamily of protease inhibitors. (wikipedia.org)
  • Serpins act as irreversible, suicide inhibitors by trapping an intermediate of the protease's catalytic mechanism. (wikipedia.org)
  • There are two lines of evidence to indicate that uterine serpins do not function as protease inhibitors. (wikipedia.org)
  • Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in plants where their function is not known. (curehunter.com)
  • By comparing the transcriptomic signatures of human brain metastatic subpopulations, Valiente and colleagues associated genes encoding for serpins-a family of potent inhibitors of plasminogen activator-with the brain metastatic phenotype. (sciencemag.org)
  • A gene on chromosome 3q26.1 that encodes a member of the serpin superfamily of serine proteinase inhibitors that inhibits tissue-type plasminogen activator. (thefreedictionary.com)
  • As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. (rndsystems.com)
  • The serpins differ from the many other families of serine protease inhibitors in that they undergo a profound change in topology in order to entrap their target protease in an irreversible complex. (portlandpress.com)
  • Background: Serpins are a superfamily of serine peptidase inhibitors that participate in the regulation of many physiological and cell peptidase-mediated processes in all organisms (e.g. in blood clotting, complement activation, fibrinolysis, inflammation, and programmed cell death). (muni.cz)
  • Serine proteinase inhibitors (serpins) finely regulate serine proteinase activity via a suicide substrate-like inhibitory mechanism. (biomedcentral.com)
  • The serpins belong to the superfamily of serine peptidase inhibitors and are expressed by many organisms ranging from plants to vertebrates. (biomedcentral.com)
  • The superfamily of serine protease inhibitors (SERPINs) plays a key role in controlling the activity of proteinases in diverse biological processes. (ovid.com)
  • Serine proteinase inhibitors (serpins) are one of the major regulators of cellular proteolysis. (peerj.com)
  • Serpins comprise the largest superfamily of peptidase inhibitors and are well known as regulators of hemostasis and thrombolysis. (monash.edu)
  • The serpins is an acronymic name given to a family of serine protease inhibitors that share a complex, but well conserved, tertiary structure. (cam.ac.uk)
  • The reason for the evolutionary success of the serpins is their possession, uniquely amongst the many families of serine protease inhibitors, of a mobile reactive site loop. (cam.ac.uk)
  • Serpins are a broadly distributed family of protease inhibitors that use a conformational change to inhibit target enzymes. (edu.au)
  • Serpins generally serve while inhibitors that utilize a mobile reactive center loop (RCL) as bait to trap protease targets. (healthandwellnesssource.org)
  • One of the hallmarks of such cascades is their regulation by serine protease inhibitors (serpins). (ox.ac.uk)
  • We have investigated the interaction of two serpins with members of the subtilisin family, proteinases that possess a similar catalytic mechanism to the chymotrypsins, but a totally different scaffold. (nih.gov)
  • These aspects of the subtilisin interaction are all observed during inhibition of chymotrypsin family members by serpins, indicating that serpins accomplish inhibition of these two distinct proteinase families by the same mechanism. (nih.gov)
  • The structures indicated that the inhibitory mechanism involved an unusual conformational change and prompted the subsequent structural focus of serpin studies. (wikipedia.org)
  • Nonetheless, the enzymatic chemistry is similar, and the mechanism of inhibition by serpins is the same for both classes of protease. (wikipedia.org)
  • Bovine uterine serpin does inhibit pepsin but probably through a mechanism distinct from the prototypical mechanism used by serpins. (wikipedia.org)
  • Serpins use an extraordinary mechanism of protease inhibition that depends on a rapid and marked conformational change and causes destruction of the covalently linked protease. (nih.gov)
  • The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. (rcsb.org)
  • This study presents a mechanism for serpin-mediated inhibition of plasminogen activator that enables both lung and breast metastatic cancer cells to colonialize the brain and shields them from apoptosis. (sciencemag.org)
  • found that serpin knockdown in metastatic cell lines removed the serpin protection mechanism in mice brain slice assays. (sciencemag.org)
  • We now report the mechanism and kinetics of this unusual inhibition of a cysteine proteinase by a member of the serpin superfamily previously thought to inhibit serine proteinase only. (embl-heidelberg.de)
  • The downside of the mobile mechanism of the serpins is their vulnerability to aberrant intermolecular ϐ-linkages, resulting in various disorders from cirrhosis to thrombosis. (portlandpress.com)
  • We also review the application of both hydrogen/deuterium exchange and ion mobility mass spectrometry to probe the mechanism of serpin polymerization and the structure of serpin polymers. (caltech.edu)
  • Our findings suggest that ANISERP is an internal Anisakis regulatory serpin and that the inhibitory activity against thrombin depends on a suicide substrate-like inhibitory mechanism, similar to that described for human antithrombin (AT)-III. (biomedcentral.com)
  • The conserved three-dimensional structure of serpins is composed of three β -sheets ( β A- β C) and 8-9 α -helices ( α A- α I). The hallmark of the serpin inhibitory mechanism is a large-scale conformational change involving the reactive center loop (RCL). (peerj.com)
  • Studies using model organisms, from plants to vertebrates, now show that serpins and their unique inhibitory mechanism and conformational flexibility are exploited to control proteolysis in molecular pathways associated with cell survival, development and host defense. (monash.edu)
  • Thus, the ancestral serpin and the origin of the serpin inhibitory mechanism remain obscure. (edu.au)
  • [17] [18] The structures indicated that the inhibitory mechanism involved an unusual conformational change and prompted the subsequent structural focus of serpin studies. (whatit.info)
  • PAI-2 uses a suicide inhibition mechanism (a common mechanism for serpins) to irreversibly inactivate tPA and urokinase. (wikipedia.org)
  • The mechanism of cysteine protease inhibition is similar except no stable serpin-cysteine protease complex is observed. (healthandwellnesssource.org)
  • Determination of the structures of inhibitory serpins in multiple conformational states permits a detailed analysis of the mechanism of the S → R transition, and of the way in which a single sequence can form two stabilised states of different topology. (elsevier.com)
  • CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. (rcsb.org)
  • The inhibition of ICE by CrmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. (embl-heidelberg.de)
  • Although named after its complement inhibitory activity, Serpin G1 also inhibits proteinases of the fibrinolytic, clotting, and kinin pathways. (gbiosciences.com)
  • Background- Protease nexin-1 (PN-1) is a serpin that inhibits plasminogen activators, plasmin, and thrombin. (inserm.fr)
  • Here, we revealed that the serpin Spn4 from Drosophila melanogaster inhibits the activity of all the PCs found in the constitutive secretory pathway and represses the metastatic potential of the colon cancer cells HT-29 and CT-26. (uni-bielefeld.de)
  • The designation of uterine serpins as SERPINA14 is based on their classification as a highly-divergent group of the α1-antitrypsin or A clade. (wikipedia.org)
  • In other analyses, uterine serpins have been considered as a separate clade in the serpin superfamily. (wikipedia.org)
  • In the 2000s, a systematic nomenclature was introduced in order to categorise members of the serpin superfamily based on their evolutionary relationships. (wikipedia.org)
  • It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. (rcsb.org)
  • When inhibition occurs, the rate of formation and stability of the complexes are similar to those formed between serpins and chymotrypsin family members. (nih.gov)
  • However, inhibition of subtilisins is characterized by large partition ratios where more than four molecules of each serpin are required to inhibit one subtilisin molecule. (nih.gov)
  • Another possible role for uterine serpins is in the inhibition of immune cell proliferation during pregnancy to provide protection for the allogeneically-distinct conceptus. (wikipedia.org)
  • Serpins thus provide stoichiometric, irreversible inhibition, and their dependence on conformational change is exploited for signalling and clearance. (nih.gov)
  • Recent crystal structures reveal the intricate conformational rearrangements involved in protease inhibition, activity modulation and the unique molecular pathology of the remarkable shape-shifting serpins. (nih.gov)
  • Inhibition of interleukin-1 beta converting enzyme by the cowpox virus serpin CrmA. (embl-heidelberg.de)
  • This ability to provide effectively irreversible inhibition explains the selection of the serpins to control the proteolytic cascades of higher organisms. (portlandpress.com)
  • The main function of Serpin G1 is the inhibition of the complement system to prevent spontaneous activation. (gbiosciences.com)
  • Cytotoxicity assays showed that deletion of the VV serpin genes B13R and B22R and other genes between B13R and B24R did not increase the level of lysis, indicating that these genes are not involved in inhibition of antigen presentation of the epitopes tested. (ox.ac.uk)
  • Native, metastable serpins inherently tend to undergo stabilizing conformational transitions in mechanisms of health (e.g., enzyme inhibition) and disease (serpinopathies). (ucl.ac.uk)
  • Inhibition of serine protease occurs when the mobile RCL of the serpin forms a covalent complex with the target serine protease, thereby blocking the activity of the protease. (healthandwellnesssource.org)
  • The inhibition reaction can generate two reaction products, the covalent 1:1 serpin-protease complex, or the RCL-cleaved serpin (stably inactive serpin)2,15. (healthandwellnesssource.org)
  • Among the clades in which uterine serpin genes exist are the Cetartiodactyla (dolphin, cow, water buffalo, sheep, goat, pig), Perissodactyla (horse) and some carnivores (dog, giant panda) The uterine serpin gene is not expressed in all carnivores since the only uterine serpin identified in the cat is a pseudogene. (wikipedia.org)
  • The major regulator of uterine serpin gene expression is progesterone. (wikipedia.org)
  • In the cow, estrogen can also increase uterine serpin gene expression. (wikipedia.org)
  • A single nucleotide polymorphism at position 1269 of the bovine uterine serpin gene has been associated with productive life in cattle populations. (wikipedia.org)
  • In this study, we characterized the serpin ( SRPN ) gene family in the mosquito Anopheles gambiae , the major malaria vector in Sub-Saharan Africa. (pubmedcentralcanada.ca)
  • We completed sequencing of cDNAs for the A. gambiae serpins to obtain complete coding sequence information and to verify or improve gene predictions. (pubmedcentralcanada.ca)
  • This article reports the gene sequencing, cloning, expression and preliminary biochemical and bioinformatically-based structural characterization of a new Anisakis serpin (ANISERP). (biomedcentral.com)
  • α1-antitrypsin (SERPINA1), the most studied member of this family, is encoded by a gene located within the proximal 14q32.1 SERPIN subcluster, together with the highly homologous α1-antitrypsin-like sequence (SERPINA2), which was previously proposed to be a pseudogene. (ovid.com)
  • We also show that the SERPINA2 gene is expressed and probably encodes a functional SERPIN. (ovid.com)
  • NCBI/Uniprot data below describe general gene information for serpin-1 . (mybiosource.com)
  • Mutations in this gene are associated with nonsyndromic progressive hearing loss, suggesting that this serpin plays an important role in the inner ear in the protection against leakage of lysosomal content during stress, and that loss of this protection results in cell death and sensorineural hearing loss. (creative-biogene.com)
  • Among these clones, there are 11 variants of the reactive center region, each encoded by a different version of the ninth exon in the serpin gene. (symbolical.us)
  • Thus, evolution of this insect serpin gene has resulted from duplication and sequence divergence of only the exon encoding the reactive site. (symbolical.us)
  • Your search returned 62 serpin-1 ELISA ELISA Kit across 1 supplier. (biocompare.com)
  • ELISA: Human Serpin A8/AGT ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. (assaysolution.com)
  • Description: For Quantitative Detection of human Serpin G1 in cell culture supernates, serum and plasma (heparin, EDTA). (cromauv.org)
  • Protease inhibitory activity in blood plasma was first reported in the late 1800s, but it was not until the 1950s that the serpins antithrombin and alpha 1-antitrypsin were isolated. (wikipedia.org)
  • The regulatory advantages provided by structural mobility are best illustrated by the heparin activation mechanisms of the plasma serpins antithrombin and heparin cofactor II. (nih.gov)
  • After a protease cleaves the RCL at the scissile bond between amino acid residues designated P1 and P1′, the serpin undergoes a large conformational change, and the protease is trapped with its active site distorted, resulting in its inactivation ( Gettins, 2007 ). (pubmedcentralcanada.ca)
  • In this chapter, we review the principles of the two most commonly employed structural mass spectrometry techniques-hydrogen/deuterium exchange and chemical footprinting-and describe their application to studying serpin flexibility, stability, and conformational change in solution. (caltech.edu)
  • Insect serpins have been found to regulate the activation of the Toll pathway and the PPO cascade (for recent review observe (16). (biobeds.info)
  • Target protease specificity of the viral serpin CrmA. (nih.gov)
  • The Human Serpin A1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. (thermofisher.com)
  • Serpin F1/PEDF Monoclonal antibody specifically detects Serpin F1/PEDF in Human samples. (fishersci.com)
  • Antibody binding rendered β-sheet A - on the opposite face of the molecule - more liable to adopt an 'open' state, mediated by changes distal to the breach region and proximal to helix F. The allosteric propagation of induced changes through the molecule was evidenced by an increased rate of peptide incorporation and destabilisation of pre-formed serpin-enzyme complex following mAb 4B12 binding. (biochemj.org)
  • This antibody detects specifically mouse Serpin C1 with WB. (angioproteomie.com)
  • The serpin does not inhibit serine peptidases, unlike most serpins, but has been shown to inhibit the lysosomal cysteine endopeptidase cathepsin L [ PMID: 12685865 ]. (ebi.ac.uk)
  • Most notably, Serpin G1 play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. (gbiosciences.com)
  • Secondary structure of OVAX is similar to that of ovalbumin, but the three-dimensional model of OVAX reveals the presence of a cluster of exposed positive charges, which potentially explains the affinity of this ov-serpin for heparin, as opposed to ovalbumin. (sigmaaldrich.com)
  • As a member of the ovalbumin-related serpin family, PAI-2 is genetically similar to chicken ovalbumin ( Gallus gallus ), and is a close mammalian homolog. (wikipedia.org)
  • Phylogenetic analyses confirmed that SRPN1, 2, 3, 8, 9 and 10 formed phylogenetic clusters with known inhibitory serpins from Drosophila melanogaster and Manduca sexta . (pubmedcentralcanada.ca)
  • In insects, serpins have been identified in several species, but most studies of insect serpins are from the fruit fly Drosophila melanogaster and the tobacco hornworm Manduca sexta . (pubmedcentralcanada.ca)
  • A serpin regulates dorsal-ventral axis formation in the Drosophila embryo. (ox.ac.uk)
  • Since this serpin has been recently shown to restrain an immune reaction in the blood of Drosophila, it demonstrates that proteolysis can be regulated by the same serpin in different biological contexts. (ox.ac.uk)
  • In addition, several key amino acids in the hinge region of the reactive center loop which are important for protease inhibitory activity have not been conserved in uterine serpins. (wikipedia.org)
  • After 16h of serum starvation, the cells were pretreated with full-length recombinant human PEDF, PN-1 and PN-1-[R346A], an altered version at its serpin reactive center, as well as synthetic peptides derived from PEDF termed 34mer, 44mer and 17mer, and from PN-1 termed 17mer and incubated at 37C for 2 h. (arvojournals.org)
  • Mutually exclusive exon use and reactive center diversity in insect serpins. (symbolical.us)
  • We have isolated 38 cDNA clones for serpins from a lepidopteran insect, Manduca sexta, and have found that they are identical in sequence, except for a region encoding the carboxyl-terminal 40-45 residues, which includes the reactive center. (symbolical.us)
  • Belongs to the serpin family. (abcam.com)
  • Human corticosteroid binding globulin (CBG, transcortin), also referred to as SerpinA6, belongs to the serpin superfamily. (biovendor.com)
  • SERPINE2 is a secreted glycoprotein which belongs to the serpin family. (acrobiosystems.com)
  • The neuroprotective and angiogenesis inhibitory serpin , PEDF: new insights into phylogeny, function, and signaling. (thefreedictionary.com)
  • PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. (prospecbio.com)
  • The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. (prospecbio.com)
  • To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. (prospecbio.com)
  • Two serpins, pigment epithelium-derived factor (PEDF) and protease nexin-1 (PN-1), have neurotrophic properties and are present extracellularly in the retina. (arvojournals.org)
  • The two serpins PEDF and PN-1 share protective properties against NaIO 3 -mediated injury. (arvojournals.org)
  • Serpins are important regulators of proteolytic pathways with an anti-protease activity that involves a conformational transition from a metastable to a hyperstable state. (biochemj.org)
  • The protease targets of intracellular inhibitory serpins have been difficult to identify, since many of these molecules appear to perform overlapping roles. (wikipedia.org)
  • SERPINs are responsible for a diverse set of intracellular and extracellular functions including cellular differentiation, tumor suppression, apoptosis, and cell migration. (cags.org.ae)
  • An intracellular serpin protects worm cells from a lysosomally mediated form of necrosis. (sciencemag.org)
  • The assay will exclusively recognize both natural and recombinant Hu Serpin A1. (thermofisher.com)
  • However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA. (rcsb.org)
  • We reported previously that human interleukin-1 beta converting enzyme (ICE) is regulated by the CrmA serpin encoded by cowpox virus. (embl-heidelberg.de)
  • CrmA possesses several characteristics typical of a number of inhibitory serpins. (embl-heidelberg.de)
  • CrmA contains a novel leucine-rich nuclear export signal (NES) that is functionally conserved in the anti-apoptotic cellular serpin PI-9. (biochemj.org)
  • Serpins act as suicide substrates and are cleaved by their target protease, forming an essentially irreversible 1:1 complex. (ox.ac.uk)
  • Serpins play a crucial role in regulating protease cascades involved in immunity of arthropods. (k-state.edu)
  • This assay has high sensitivity and excellent specificity for detection of human Serpin A8/AGT. (assaysolution.com)
  • The inactive serpin PN-1-[R346A] decreased cytotoxicity and increased cell viability similar to its wild type counterpart PN-1. (arvojournals.org)
  • There are two possible biological roles for uterine serpins during pregnancy. (wikipedia.org)
  • Vertebrate serpins are classified into six groups (V1-V6), based on three independent biological features-genomic organization, diagnostic amino acid sites and rare indels. (peerj.com)
  • In addition, an increasing number of non-inhibitory serpins are emerging as important elements within a diversity of biological systems by serving as chaperones, hormone transporters or anti-angiogenic factors. (monash.edu)
  • Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. (rndsystems.com)
  • Vaccinia virus serpins B13R and B22R do not inhibit antigen presentation to class I-restricted cytotoxic T lymphocytes. (ox.ac.uk)
  • Description: For quantitative detection of human Serpin A12 in cell culture supernates, serum and plasma (heparin, EDTA) and urine. (cromauv.org)
  • Description: For quantitative detection of human Serpin A7 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). (cromauv.org)
  • With the exception of Manduca serpin-2, they all are involved in regulation of the prophenoloxidase (PPO) activation cascade, an innate immune response, but they are likely to have additional functions not yet discovered. (pubmedcentralcanada.ca)
  • when associated with an inter-molecular interaction, this yields linear polymers of hyperstable serpin molecules, which accumulate at the site of synthesis. (biochemj.org)
  • Serpins are conformationally labile and many of the disease-linked mutations of serpins result in misfolding or in pathogenic, inactive polymers. (edu.au)
  • A single-chain serpin glycoprotein that acts as a key regulator of clotting through its inhibitory effect on thrombin and factor Xa. (thefreedictionary.com)