Keratins: A class of fibrous proteins or scleroproteins that represents the principal constituent of EPIDERMIS; HAIR; NAILS; horny tissues, and the organic matrix of tooth ENAMEL. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. alpha-Keratins have been classified into at least 20 subtypes. In addition multiple isoforms of subtypes have been found which may be due to GENE DUPLICATION.Keratins, Type II: A keratin subtype that includes keratins that are generally larger and less acidic that TYPE I KERATINS. Type II keratins combine with type I keratins to form keratin filaments.Keratins, Hair-Specific: Keratins that are specific for hard tissues such as HAIR; NAILS; and the filiform papillae of the TONGUE.Keratins, Type I: A keratin subtype that includes keratins that are generally smaller and more acidic that TYPE II KERATINS. Type I keratins combine with type II keratins to form keratin filaments.Keratin-8: A type II keratin found associated with KERATIN-18 in simple, or predominately single layered, internal epithelia.Intermediate Filaments: Cytoplasmic filaments intermediate in diameter (about 10 nanometers) between the microfilaments and the microtubules. They may be composed of any of a number of different proteins and form a ring around the cell nucleus.Keratin-14: A type I keratin that is found associated with the KERATIN-5 in the internal stratified EPITHELIUM. Mutations in the gene for keratin-14 are associated with EPIDERMOLYSIS BULLOSA SIMPLEX.Keratin-10: A type I keratin that is found associated with the KERATIN-1 in terminally differentiated epidermal cells such as those that form the stratum corneum. Mutations in the genes that encode keratin-10 have been associated with HYPERKERATOSIS, EPIDERMOLYTIC.Epidermolysis Bullosa Simplex: A form of epidermolysis bullosa characterized by serous bullae that heal without scarring. Mutations in the genes that encode KERATIN-5 and KERATIN-14 have been associated with several subtypes of epidermolysis bullosa simplex.Hyperkeratosis, Epidermolytic: A form of congenital ichthyosis inherited as an autosomal dominant trait and characterized by ERYTHRODERMA and severe hyperkeratosis. It is manifested at birth by blisters followed by the appearance of thickened, horny, verruciform scales over the entire body, but accentuated in flexural areas. Mutations in the genes that encode KERATIN-1 and KERATIN-10 have been associated with this disorder.Keratin-1: A type II keratin that is found associated with the KERATIN-10 in terminally differentiated epidermal cells such as those that form the stratum corneum. Mutations in the genes that encode keratin-1 have been associated with HYPERKERATOSIS, EPIDERMOLYTIC.Keratin-5: A type II keratin that is found associated with the KERATIN-14 in the internal stratified EPITHELIUM. Mutations in the gene for keratin-5 are associated with EPIDERMOLYSIS BULLOSA SIMPLEX.Epidermis: The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).Keratin-18: A type I keratin found associated with KERATIN-8 in simple, or predominately single layered, internal epithelia.Keratinocytes: Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.Hair: A filament-like structure consisting of a shaft which projects to the surface of the SKIN from a root which is softer than the shaft and lodges in the cavity of a HAIR FOLLICLE. It is found on most surfaces of the body.Keratin-16: A type I keratin expressed in a variety of EPITHELIUM, including the ESOPHAGUS, the TONGUE, the HAIR FOLLICLE and NAILS. Keratin-16 is normally found associated with KERATIN-6. Mutations in the gene for keratin-6 have been associated with PACHYONYCHIA CONGENITA, TYPE 1.Intermediate Filament Proteins: Filaments 7-11 nm in diameter found in the cytoplasm of all cells. Many specific proteins belong to this group, e.g., desmin, vimentin, prekeratin, decamin, skeletin, neurofilin, neurofilament protein, and glial fibrillary acid protein.Keratoderma, Palmoplantar: Group of mostly hereditary disorders characterized by thickening of the palms and soles as a result of excessive keratin formation leading to hypertrophy of the stratum corneum (hyperkeratosis).Keratin-17: A type I keratin found associated with KERATIN-6 in rapidly proliferating squamous epithelial tissue. Mutations in the gene for keratin-17 have been associated with PACHYONYCHIA CONGENITA, TYPE 2.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Keratin-2: A type II keratin found expressed in the upper spinous layer of epidermal KERATINOCYTES. Mutations in genes that encode keratin-2A have been associated with ICHTHYOSIS BULLOSA OF SIEMENS.Keratin-13: A type I keratin that is found associated with the KERATIN-4 in the internal stratified EPITHELIUM. Defects in gene for keratin 13 cause HEREDITARY MUCOSAL LEUKOKERATOSIS.Epithelium: One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.Hair Follicle: A tube-like invagination of the EPIDERMIS from which the hair shaft develops and into which SEBACEOUS GLANDS open. The hair follicle is lined by a cellular inner and outer root sheath of epidermal origin and is invested with a fibrous sheath derived from the dermis. (Stedman, 26th ed) Follicles of very long hairs extend into the subcutaneous layer of tissue under the SKIN.Hoof and Claw: Highly keratinized processes that are sharp and curved, or flat with pointed margins. They are found especially at the end of the limbs in certain animals.Keratin-15: A type I keratin found in the basal layer of the adult epidermis and in other stratified epithelia.Hair Diseases: Diseases affecting the orderly growth and persistence of hair.Keratin-6: A type II keratin found associated with KERATIN-16 or KERATIN-17 in rapidly proliferating squamous epithelial tissue. Mutations in gene for keratin-6A and keratin-6B have been associated with PACHYONYCHIA CONGENITA, TYPE 1 and PACHYONYCHIA CONGENITA, TYPE 2 respectively.Desmosomes: A type of junction that attaches one cell to its neighbor. One of a number of differentiated regions which occur, for example, where the cytoplasmic membranes of adjacent epithelial cells are closely apposed. It consists of a circular region of each membrane together with associated intracellular microfilaments and an intercellular material which may include, for example, mucopolysaccharides. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990; Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Feathers: Flat keratinous structures found on the skin surface of birds. Feathers are made partly of a hollow shaft fringed with barbs. They constitute the plumage.Vimentin: An intermediate filament protein found in most differentiating cells, in cells grown in tissue culture, and in certain fully differentiated cells. Its insolubility suggests that it serves a structural function in the cytoplasm. MW 52,000.Pachyonychia Congenita: A group of inherited ectodermal dysplasias whose most prominent clinical feature is hypertrophic nail dystrophy resulting in PACHYONYCHIA. Several specific subtypes of pachyonychia congenita have been associated with mutations in genes that encode KERATINS.Keratin-12: A type I keratin that is found associated with the KERATIN-3 in the CORNEA and is regarded as a marker for corneal-type epithelial differentiation. Mutations in the gene for keratin-12 have been associated with MEESMANN CORNEAL EPITHELIAL DYSTROPHY.Dipodomys: A genus of the family Heteromyidae which contains 22 species. Their physiology is adapted for the conservation of water, and they seldom drink water. They are found in arid or desert habitats and travel by hopping on their hind limbs.Keratin-9: A type II keratin found predominantly expressed in the terminally differentiated EPIDERMIS of palms and soles. Mutations in the gene for keratin 9 are associated with KERATODERMA, PALMOPLANTAR, EPIDERMOLYTIC.Cytoskeleton: The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.Desmoplakins: Desmoplakins are cytoskeletal linker proteins that anchor INTERMEDIATE FILAMENTS to the PLASMA MEMBRANE at DESMOSOMES.Keratoderma, Palmoplantar, Epidermolytic: An autosomal dominant hereditary skin disease characterized by epidermolytic hyperkeratosis that is strictly confined to the palms and soles. It has been associated with mutations in the gene that codes for KERATIN-9.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Nail Diseases: Diseases of the nail plate and tissues surrounding it. The concept is limited to primates.Keratin-19: A type I keratin found associated with KERATIN-7 in ductal epithelia and gastrointestinal epithelia.Mallory Bodies: Cytoplasmic hyaline inclusions in HEPATOCYTES. They are associated with ALCOHOLIC STEATOHEPATITIS and non-alcoholic STEATOHEPATITIS, but are also present in benign and malignant hepatocellular neoplasms, and metabolic, toxic, and chronic cholestatic LIVER DISEASES.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Nails, Malformed: Deformities in nail structure or appearance, including hypertrophy, splitting, clubbing, furrowing, etc. Genetic diseases such as PACHYONYCHIA CONGENITA can result in malformed nails.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Corneal Dystrophy, Juvenile Epithelial of Meesmann: An autosomal dominant form of hereditary corneal dystrophy due to a defect in cornea-specific KERATIN formation. Mutations in the genes that encode KERATIN-3 and KERATIN-12 have been linked to this disorder.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Papilloma: A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.beta-Keratins: Keratins that form into a beta-pleated sheet structure. They are principle constituents of the corneous material of the carapace and plastron of turtles, the epidermis of snakes and the feathers of birds.Wool: The hair of SHEEP or other animals that is used for weaving.Skin DiseasesGriseofulvin: An antifungal agent used in the treatment of TINEA infections.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
"Sequences and differential expression of three novel human type-II hair keratins". Differentiation. 61 (3): 187-94. doi:10.1046 ... The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which ... "Entrez Gene: KRT83 keratin 83". van Steensel MA, Steijlen PM, Bladergroen RS, Vermeer M, van Geel M (March 2005). "A missense ... Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins". J. Biol. ...
"Sequences and differential expression of three novel human type-II hair keratins". Differentiation. 61 (3): 187-94. doi:10.1046 ... The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which ... "Entrez Gene: KRT85 keratin 85". Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein ... Keratin, type II cuticular Hb5 is a protein that in humans is encoded by the KRT85 gene. ...
1995). "Sequence data and chromosomal localization of human type I and type II hair keratin genes". Exp. Cell Res. 220 (2): 357 ... 1997). "Sequences and differential expression of three novel human type-II hair keratins". Differentiation. 61 (3): 187-94. doi ... All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB1 and KRTHB3, is found primarily in ... The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which ...
These studies were published in the absence of knowledge of a keratin sequence. The first keratin sequences were determined by ... Hanukoglu I, Fuchs E (Nov 1982). "The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of ... "The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins". Cell. 33 ... Based on sequence and secondary structure prediction analyses identified the coiled-coil domains of keratins. These models have ...
Bowden PE, Hainey S, Parker G, Hodgins MB (Jul 1994). "Sequence and expression of human hair keratin genes". Journal of ... The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein ... "A splice site mutation in the gene of the human type I hair keratin hHa1 results in the expression of a tailless keratin ... Keratin, type I cuticular Ha1 is a protein that in humans is encoded by the KRT31 gene. ...
"Sequence data and chromosomal localization of human type I and type II hair keratin genes". Exp Cell Res. 220 (2): 357-62. doi: ... The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein ... "Entrez Gene: KRT32 keratin 32". Yu J, Yu DW, Checkla DM, et al. (1993). "Human hair keratins". J. Invest. Dermatol. 101 (1 ... Keratin, type I cuticular Ha2 is a protein that in humans is encoded by the KRT32 gene. ...
Bowden PE, Hainey S, Parker G, Hodgins MB (1995). "Sequence and expression of human hair keratin genes". J. Dermatol. Sci. 7 ... All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in ... The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which ... "Sequence data and chromosomal localization of human type I and type II hair keratin genes". Exp Cell Res. 220 (2): 357-62. doi: ...
"The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins". Cell. 33 ... July - Determination of the first sequences of type I and type II keratins and prediction of the α-helical domain structure of ...
Analysis of a second keratin sequence revealed that the two types of keratins share only about 30% amino acid sequence homology ... "The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins". Cell. 33 ... "The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate ... Regardless of the group, keratins are either acidic or basic. Acidic and basic keratins bind each other to form acidic-basic ...
Hanukoglu I, Fuchs E (Nov 1982). "The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of ... "The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins". Cell. 33 ... where he cloned and sequenced cDNAs coding for cytoskeletal proteins, actin and alpha keratins. He elucidated the first ... By sequence analyses of the large oxidoreductase type of enzyme families, he noted that the FAD-binding site is a classical ...
"Complete genome sequence of a keratin-degrading bacterium Chryseobacterium gallinarum strain DSM 27622(T) isolated from chicken ... Chryseobacterium gallinarum has the ability to degrade keratin. Kämpfer, P; Poppel, MT; Wilharm, G; Busse, HJ; McInroy, JA; ...
A scleroprotein's peptide sequence often has limited residues with repeats; these can form unusual secondary structures, such ... The structures often feature cross-links between chains (e.g., cys-cys disulfide bonds between keratin chains). Scleroproteins ... There are many scleroprotein superfamilies including keratin, collagen, elastin, and fibroin. The roles of such proteins ...
"The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins". Cell. 33 ... "The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate ... Her first publications there reported the first cloning and sequencing of keratin cDNAs that characterized the two types of ... keratins. , At the University of Chicago she was mentored and befriended by Janet Rowley and Susan Lindquist, and eventually ...
... subtilis genome was sequenced, no ortholog of human keratin was detected.[11] The cortex lies beneath the spore coat and ... 1967). "The complete genome sequence of the gram-positive bacterium Bacillus subtilis". Nature. 390 (6657): 249-56. doi:10.1038 ... which Kadota and Iijima speculated might be formed from a keratin-like protein.[9] However, after further studies this group ... concluded that the structure of the spore coat protein was different from keratin.[10] When the B. ...
BROWN, C. H.; Keratins in Invertebrates; Nature 166, 439 (09 September 1950); doi:10.1038/166439a0 ... Weber, Klaus et al; "Amino acid sequences and homopolymer-forming ability of the intermediate filament proteins from an ... at least some of the cross-linking is by disulphide bonds reminiscent of protein cross-linking in the formation of keratin.[3] ... keratins are a fairly well-defined set of proteins comprising particular chains in different proportions cross-linked in ...
The DNA sequence of the protein has sections very similar to the eukaryotic keratin and lamin proteins, mostly involving the ... lacks certain consensus sequence elements at the ends of the rod domain which are conserved in animal lamin and keratin ... Both intermediate filament and crescentin proteins have a primary sequence including four α-helical segments along with non-α- ... its sequence mostly consists of a pattern of 7 repeated residues which form a coiled-coil structure. ...
This sequence change leads to an amino acid substitution in the highly conserved helix initiation motif of the K6hf rod domain ... A common polymorphism in a keratin gene (K6hf) has been linked to PFB, suggesting that it may be a genetic risk factor. ... McLean, W. H. I. (2004). "Close Shave for a Keratin Disorder-K6hf Polymorphism Linked to Pseudofolliculitis Barbae". Journal of ... "An Unusual Ala12Thr Polymorphism in the 1A alpha-Helical Segment of the Companion Layer-Specific Keratin K6hf: Evidence for a ...
... keratins, vimentin, neurofilaments, and desmin. Although tubulin-like proteins share some amino acid sequence similarity, their ... made of keratin. Keratin is present in general in epithelial cells. neurofilaments of neural cells. made of lamin, giving ... Keratin intermediate filaments in epithelial cells provide protection for different mechanical stresses the skin may endure. ... Although the evolutionary relationships are so distant that they are not obvious from protein sequence comparisons alone, the ...
The surfaces of PEG polymers are easily modified with peptide sequences that can attract cells for adhesion and could therefore ... RNTs can emulate skin structures such as collagen and keratin and self-assemble when injected into the body. This type of ...
They mainly attack the disulfide (-S-S-) bond of the keratin substrate (Bockel et al., 1995). Keratinase production in various ... they were defined as a serine proteases due to high sequence homology with alkaline protease, and their inhibition by serine ... 1959) attempted to isolate some bacteria that are able to degrade keratin. He isolated organism from the contents of ... Keratinases are proteolytic enzymes that digest keratin. They were initially classified as 'proteinases of unknown mechanism' ...
This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins ... 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... 2005). "Characterization of new members of the human type II keratin gene family and a general evaluation of the keratin gene ... novel keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and 18". J. Cell Sci. 114 (Pt 14 ...
... are simply called keratins (human epithelial category). For example, cytokeratin-4 (CK-4) has been renamed keratin-4 (K4). ... The end-domain sequences of type I and II cytokeratin chains contain in both sides of the rod domain the subdomains V1 and V2, ... Cytokeratins are keratin proteins found in the intracytoplasmic cytoskeleton of epithelial tissue. They are an important ... In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell ...
This has led many authors to refer to such cross-linked proteins in invertebrates as keratin, but modern analyses have shown ... ISBN 0-412-61390-5. Weber, Klaus et al; "Amino acid sequences and homopolymer-forming ability of the intermediate filament ... at least some of the cross-linking is by disulphide bonds reminiscent of protein cross-linking in the formation of keratin. ... that the term is inappropriate; keratins are a fairly well-defined set of proteins comprising particular chains in different ...
2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ... "Entrez Gene: ODF1 outer dense fiber of sperm tails 1". Kierszenbaum AL (2002). "Keratins: unraveling the coordinated ... "Sequence, expression, and chromosomal assignment of a human sperm outer dense fiber gene". Mol Reprod Dev. 36 (4): 407-418. doi ...
2016). "Whole exome sequencing and segregation analysis confirms that a mutation in COL17A1 is the cause of epithelial ... Collagen, type XVII, alpha 1 has been shown to interact with Keratin 18, Actinin alpha 4, Dystonin, Actinin, alpha 1, CTNND1 ... Whole-exome sequencing first identified a heterozygous mutation (c.2816C>T, p.T939I) that segregated with ERED in a large ... thereby mediating keratin cytoskeleton anchorage to the cell surface at the site of the hemidesmosome". Mol. Biol. Cell. UNITED ...
上皮角蛋白(英语:Epithelial keratin). (软α-角蛋白). *type I/chromosome 17 *10 ... Lamin A/C gene and a related sequence map to human chromosomes 1q12.1-q23 and 10. Somat. Cell Mol. Genet. March 1993, 19 (2): ...
We sought the genetic causes of EB in a cohort of 512 distinct EB families by performing whole exome sequencing (WES) and using ... We sought the genetic causes of EB in a cohort of 512 distinct EB families by performing whole exome sequencing (WES) and using ... We sought the genetic causes of EB in a cohort of 512 distinct EB families by performing whole exome sequencing (WES) and using ... Epidermolysis bullosa simplex, KRT5 homozygosity, Next generation sequencing, Phenotype-genotype correlations. in Matrix ...
The type II cytokeratins are clustered in a region of chromosome 12q12-q13.Keratin 1 is specifically expressed in the spinous ... and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the ... p,The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains ... "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [ ...
Rabbit recombinant monoclonal Keratin 12/K12 antibody [EPR1609(2)]. Validated in WB and tested in Rat, Human. Cited in 2 ... Sequence similarities. Belongs to the intermediate filament family.. * Target information above from: UniProt accession Q99456 ... Anti-Keratin 12/K12 antibody [EPR1609(2)]. See all Keratin 12/K12 primary antibodies. ... Anti-Keratin 12/K12 antibody [EPR1609(2)] (ab124975) at 1/1000 dilution + Rat eyeball lysate at 10 µg. Predicted band size: 54 ...
90% identical with the sequence of this keratin. We synthesized primers based on these sequences. We constructed a cDNA library ... residues from 105 to 140 of the keratin Q90W76 are absent from the Tr65 sequence. Keratin Q90W76 has a molecular mass of 62344 ... Analysis of internal sequences of Tr65 indicated that it significantly matched the trout O. mykiss type II epidermal keratin E1 ... The protein sequence translated from this gene confirms the very strong similarity previously observed with a trout keratin ( ...
VEGA , MGI Sequence Detail. 1698. Not Applicable. polypeptide. OTTMUSP00000002389. VEGA , MGI Sequence Detail. 484. Not ... J:30054 Knapp B, et al., Three cDNA sequences of mouse type I keratins. Cellular localization of the mRNAs in normal and ... Representative Sequences. Length. Strain/Species. Flank. genomic. OTTMUSG00000002538. VEGA Gene Model , MGI Sequence Detail. ... If keratin 16 is also expressed in skin, development is normal but later alopecia, chronic skin ulcers and stratified ...
Glass C, Kim KH, Fuchs E (Dec 1985). "Sequence and expression of a human type II mesothelial keratin". The Journal of Cell ... or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene. Keratin 7 is a type II keratin ... Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are ... "Entrez Gene: KRT7 keratin 7". Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M (2003). Manual of Diagnostic Cytology (2 ...
Sequence (68) , Silk (13) , Small (479) , Space (501) , Structure (346) , Technique (80) , Three-Dimensional (11) , Turn (447) ... Keratin Quotes (1 quote) In describing a protein it is now common to distinguish the primary, secondary and tertiary structures ... Secondary structure has been assigned in broad outline to a number of librous proteins such as silk, keratin and collagen; but ... The primary structure is simply the order, or sequence, of the amino-acid residues along the polypeptide chains. This was first ...
Anti- Keratin-like protein KRT222; pAb has guaranteed Elisa titer of 1:64000 and 95% WB success rate ... Suggested Peptide Antigen for Keratin-like protein KRT222; can be found in Genscripts Peptide Antigen Database. ... Protein name and sequence of (Keratin-like protein KRT222) Protein Names. Recommended name:. Keratin-like protein KRT222 ... Protein Sequence. SEQUENCE 295 AA; 34263 MW; FBDF833092C99196 CRC64; MELSQLLNEI RANYEKLLTR NQIETVLSTR IQLEEDLSKK MDKDEEALKA ...
Keratin-5. Keratins / metabolism*. Male. Mice. Microscopy, Immunoelectron. Molecular Sequence Data. Nuclear Envelope / ... 0/Actins; 0/KRT5 protein, human; 0/Keratin-5; 0/Krt1-5 protein, mouse; 0/Krt2-5 protein, rat; 68238-35-7/Keratins ... Amino Acid Sequence. Animals. Cell Nucleus / metabolism*, ultrastructure. Cloning, Molecular. Fluorescent Antibody Technique. ... Sequence Analysis, Protein. Skin / cytology, metabolism. Spermatids / metabolism*, ultrastructure. Spermatogenesis / physiology ...
The data also support this sequence of events. There is a clear decrease in yield stress and elastic modulus of the Hookean ... hydrated alpha-keratin) to 85±19.5% for WECB. Drying WECB appears to make it more similar to hydrated alpha-keratin, but ... In hard alpha-keratin, a very stiff and brittle material, the addition of water acts as a plasticizer allowing a decrease in ... This agrees with keratin models suggesting that in the yield region there are still coiled-coil motifs unraveling, and that the ...
keratin 18. 21. 25. 9. 5. Sequence references in MGI J:259852 Mouse Genome Informatics and the WTSI Mouse Genomes Project, MGI ... Sequence Detail ID/Version MGP_CASTEiJ_G0021655 (Ensembl) Multiple Genome Viewer (MGV) Version: MGP_CASTEiJ_G0021655.Ensembl ... Sequence. description. from provider chr15:104041877-104045671, + strand. Annotation of mouse strain CAST/EiJ genome assembly ...
Keratins / genetics, metabolism. Male. Mice. Mice, Nude. Models, Biological. Molecular Sequence Data. Proteins / chemistry, ... Amino Acid Sequence. Amino Acids / analysis. Animals. Female. Fetal Tissue Transplantation. Gene Expression. Hair / growth & ... Previous Document: Genetic disorders of keratin: are scarring alopecias a sub-set?. Next Document: Hair growth-stimulating ... Hence, keratin and HSP gene expression and regulation of melanogenesis in the normal and genetically defective TTD human hair ...
Like well-differentiated LPS shows amplified sequence of Chr 12q14-15.. *Immunohistochemistry: S100+, MDM2+ and CDK4+; keratin ...
Keratin Associated Protein 6-3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... Interacts with hair keratins.. SequenceCaution:. *Sequence=BAE46366.1; Type=Erroneous initiation; Evidence={ECO:0000305}; ... In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin- ... In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin- ...
... keratin also promoted the up-regulation of a gene encoding an adhesin-like protein with a tandem repeat sequence. In silico ... rubrum conidia grown in keratin substrate and containing an interesting pattern of tandem repeat sequences related to adhesion ... Simple sequence repeats as advantageous mutators in evolution. Trends Genet. 2006;22(5):253-9.View ArticlePubMedGoogle Scholar ... Benson G. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res. 1999;27(2):573-80.View ArticlePubMed ...
The C-terminal sequences (residues 84 to 92), in particular valines 86 and 88, are important in the formation of these ... Therefore, to establish the role of sequences other than those at the N terminus, we have performed a mutational analysis of ... Mutational analysis of the human papillomavirus type 16 E1--E4 protein shows that the C terminus is dispensable for keratin ... In cultured epithelial cells the proteins associate with the keratin intermediate filaments (IFs) and, for some E4 types, e.g ...
Keratin 42 Pseudogene, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human ... Keratin 42 pseudogene:. *Hs.725790. Representative Sequences:. *AK095281. CRISPR Products. * Browse CRISPR knockouts for KRT42P ... Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics ... New consensus nomenclature for mammalian keratins. (PMID: 16831889) Schweizer J … Wright MW (The Journal of cell biology 2006) ...
In this case, the integration of a mobile element containing promoter-like sequences in a keratin gene could lead to an ... the basic hair keratin gene has been located between the keratin 7 gene and the keratin 6 gene. The structure of several type ... We have mapped four basic hair keratin genes between Krt2-7 and Krt2-6b; in humans the basic keratin genes have been recently ... sequences containing "Mus musculus type II hair keratin mRNA" as a criterion in the definition field. From this search, 209 EST ...
"Sequence of a cDNA encoding human keratin No 10 selected according to structural homologies of keratins and their tissue- ... or keratin-10 (K10) is a protein that in humans is encoded by the KRT10 gene. Keratin 10 is a type I keratin. Keratin-10 is a ... 1988). "The complete sequence of the human intermediate filament chain keratin 10. Subdomainal divisions and model for folding ... Keratin 10 has been shown to interact with AKT1. 34βE12 (keratin 903) GRCh38: Ensembl release 89: ENSG00000186395 - Ensembl, ...
dorsum keratin skin layers cells thickness alkaline molecules/. Stratum Corneum: Why do henna stains on the the palms and soles ... The sequence of lawsone from henna leaf powder is as follows:. 1) Lawsone is derived from hennocide precursors in the henna ... lawsone keratin skin layers cells days alkaline molecules/. Stratum Corneum: Why doesnt a henna stain last forever like a ... lawsone keratin skin layers cells thickness alkaline molecules/. Stratum Corneum: What are those last orange traces, and why do ...
KRT1, Keratin 1. Sequence ID:. NM_006121. RefSeqGene ID:. NG_008364. Entrez Gene ID:. 3848. ... CC] keratin filament *[CC] membrane *[CC] plasma membrane *[MF] carbohydrate binding *[MF] protein binding *[MF] receptor ...
This for instance, thats the recipe for keratin.. You can find all those sequences on the Internet. ... Your hair for instance, thats mainly keratin, just a protein.. And your muscles, they only consist of the proteins actin and ... If the coding sequence is 420 nucleotides long, how long then is the protein? ... And you probably already understand that changes in these sequences in these ...
Human keratin is a very common contaminant!!! Therefore, the following extra measures must be taken to minimize the ... Service is also provided for sequencing and identification of proteins and peptides using LC/MS-MS on a recharge basis. ... For peptide and protein sequencing and identification from SDS-PAGE gel bands, any band visible by Coomassie staining is ... This way you will increase your chances to get one or more peptide sequences and youre your protein identified. ...
Keratin Associated Protein 10-9, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... Suggested Antigen Peptide Sequences for KRTAP10-9 Gene. GenScript: Design optimal peptide antigens:. *Keratin-associated ... The DNA sequence of human chromosome 21. (PMID: 10830953) Hattori M … Chromosome 21 mapping and sequencing consortium (Nature ... In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin- ...
... subtilis genome was sequenced, no ortholog of human keratin was detected.[11] The cortex lies beneath the spore coat and ... 1967). "The complete genome sequence of the gram-positive bacterium Bacillus subtilis". Nature. 390 (6657): 249-56. doi:10.1038 ... which Kadota and Iijima speculated might be formed from a keratin-like protein.[9] However, after further studies this group ... concluded that the structure of the spore coat protein was different from keratin.[10] When the B. ...
  • We sought the genetic causes of EB in a cohort of 512 distinct EB families by performing whole exome sequencing (WES) and using an EB-targeting next-generation sequencing (NGS) panel of 21 genes. (lu.se)
  • However, there are no reports of immunohistochemical staining patterns of alpha-keratins in other tissues from reptiles. (springer.com)
  • We also studied the immunolocalization of alpha-keratins in squamous cell carcinomas (SCCs) in a bearded dragon and two loggerhead turtles. (springer.com)
  • Alpha-keratins were detected in a greater number of tissues of loggerhead turtles compared with those observed in bearded dragons. (springer.com)
  • This study provides, for the first time, information about the immunohistochemical staining patterns of alpha-keratins in normal tissues from bearded dragons and loggerhead sea turtles, and confirms the usefulness of AE1 and AE3 monoclonal antibodies in these reptile species. (springer.com)
  • Unlike other vertebrates birds and reptiles produce beta keratins, in addition to the alpha keratins which appear in the epidermis of all vertebrates. (springer.com)
  • 1983), O'Guin and Sawyer (1982) and O'Guin (1984) have shown that the alpha keratins of birds are also expressed in a tissue-specific manner, and are recognized, on Western Blots, by at least one of the monoclonal antibodies made against human alpha keratins (Sun et al. (springer.com)
  • As a proof of concept we applied the database to whole-genome DNA sequencing data of one of the clinical isolates, which allowed the validation of 116 predicted single amino acid polymorphisms and the annotation of 131 N-terminal start sites. (mcponline.org)
  • High-throughput genome sequencing technology has led to an exponential increase in the capacity to generate complete genomic data for diverse organisms ( 4 ). (mcponline.org)
  • As more and more large-scale human genome sequencing projects get completed, scientists have been able to trace with increasing confidence both the geographical movements and underlying genetic variation of human populations. (smbe.org)
  • DNA sequences and corresponding amino acid sequences from the HLA class II beta region of the human genome that are associated with insulin-dependent diabetes mellitus (IDDM) and Pemphigus vulgaris (PV) have been identified. (google.com.au)
  • When the challenge to sequence the first human genome was announced in 1988, it took approximately 15 years and $2.7 billion in 1991 dollars to produce and annotate the first complete sequence. (summitdaily.com)
  • Today, the cost of sequencing all three billion bases of DNA in the human genome has plunged to less than $4,000 (an approximately 1 million-fold decline). (summitdaily.com)
  • Scientists anticipate being able to sequence an entire human genome for less than $100 within a few years. (summitdaily.com)
  • Your future visits to the doctor's office may involve taking a genome sequence along with your blood pressure. (summitdaily.com)
  • We combined spectrometry, electron microscopy and thin-film optical modelling to describe the UV-reflecting iridescent colour of feather barbules of male blue-black grassquits ( Volatinia jacarina ), characterized by a keratin layer overlying a single melanin layer. (royalsocietypublishing.org)
  • In this work, we propose a biomechanical computational framework, which allows us to perform numerical simulations in order to mimic the behaviour of keratin filament networks as observed in microscopy data. (scch.at)
  • Among the invertebrates , arthropods such as crustaceans often have parts of their armor or exoskeleton made of keratin, sometimes in combination with chitin , which is a hard, semitransparent polysaccharide that is the main component of the shells of crustaceans, such as crabs , lobsters , and shrimp . (newworldencyclopedia.org)
  • Keratins also are found in the gastrointestinal tracts of many animals, including roundworms (who also have an outer layer made of keratin). (newworldencyclopedia.org)
  • Arthropods such as crustaceans often have parts of their armor or exoskeleton made of keratin, sometimes in combination with chitin . (wikidoc.org)
  • The baleen plates of filter-feeding whales are made of keratin. (wikidoc.org)
  • We investigated the role of keratin IFs during the epithelialization of skin wounds using a keratin 6α and 6β (K6α/K6β)-null mouse model. (rupress.org)
  • In arthropods, however, chitin is frequently modified by being embedded in a hardened proteinaceous matrix of keratin, giving a more rigid exoskeleton, than seen, for example, in the use of chitin in the soft, more pliable body wall of a caterpillar . (newworldencyclopedia.org)
  • Prediction from deduced amino acid sequences suggested that each MOMP subunit consisted of 18 β-strands connected by short periplasmic turns and long irregular external loops. (asm.org)
  • Alignment of the amino acid sequences of MOMP from different strains indicated that there were seven localized variable regions dispersed among highly conserved sequences. (asm.org)
  • Thoroughly wash anything that will come into contact with your sample (i.e. gel apparatus, staining trays, gel excising implements, gel storage equipment, and Eppendorf vials) to remove human keratins and other contaminants. (utep.edu)
  • 2008) The human keratins: biology and pathology. (miltenyibiotec.com)
  • As presently reported, two approaches were used to determine the lineage-derived fate of basal cells: (1) tracking that allows lineage-specific tagging of the keratin 14-expressing population of cells throughout prostate development in adult mice, and (2) a keratin 5 H2BGFP-label retaining model demonstrating that slowly cycling cells are represented in only a subfraction of keratin 5-expressing cells after hormone/castration manipulation. (hindawi.com)
  • A transcriptional profiling analysis was conducted using a custom oligonucleotide-based microarray by comparing T. rubrum conidia grown on elastin and keratin substrates. (biomedcentral.com)
  • Molecular markers in keratins from Mysticeti whales for species identification of baleen in museum and archaeological collections. (bireme.br)
  • None of these factors were clearly linked to avian keratin disorder (AKD) in Black-capped Chickadees or other affected species. (usgs.gov)
  • Although these studies suggest that keratins are important for MB development, the relationship between single or dual K8 and K18 overexpression and MB formation under basal and stimulated conditions remains unclear. (rupress.org)
  • In the cutaneous SCCs of both sea turtles, acidic alpha-keratin was detected in the basal and suprabasal layers, and in all of the invasive neoplastic cords, while basic alpha-keratin was mainly detected in the invasive neoplastic cords. (springer.com)
  • Luminal and basal populations are identifiable through expression of specific keratins. (hindawi.com)
  • Basal cells express keratins 5 and 14 only, but, if luminal cells develop, they lose these keratins and express keratins 8 and 18 [ 3 - 5 ]. (hindawi.com)
  • Keratin 15 has been associated with diseases such as malignant syringoma and morpheaform basal cell carcinoma. (biolegend.com)