A family of GLYCOSYLPHOSPHATIDYLINOSITOL-anchored, cell-surface heparan sulfate proteoglycans that may play a role in CELL GROWTH PROCESSES and CELL DIFFERENTIATION by modulating ligand-receptor interactions.
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
A family of transmembrane glycoproteins that contain a short cytoplasmic domain, a single-span transmembrane domain, and an extracellular domain with heparin sulfate and CHONDROITIN SULFATE chains. Syndecans interact with a variety of heparin-binding INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS and may play a role in modulating cellular signaling during EMBRYONIC DEVELOPMENT, tumorigenesis, and angiogenesis.
Glycoproteins which have a very high polysaccharide content.
A syndecan that interacts with EXTRACELLULAR MATRIX PROTEINS and plays a role CELL PROLIFERATION and CELL MIGRATION.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
A family of intercellular signaling proteins that play and important role in regulating the development of many TISSUES and organs. Their name derives from the observation of a hedgehog-like appearance in DROSOPHILA embryos with genetic mutations that block their action.
Glycoproteins found on the membrane or surface of cells.
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
A species of fruit fly much used in genetics because of the large size of its chromosomes.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Antibodies produced by a single clone of cells.
A genus in the family ORTHOMYXOVIRIDAE causing influenza and other diseases in humans and animals. It contains many strains as well as antigenic subtypes of the integral membrane proteins hemagglutinin (HEMAGGLUTININS) and NEURAMINIDASE. The type species is INFLUENZA A VIRUS.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Proteins prepared by recombinant DNA technology.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.
The study and practice of medicine by direct examination of the patient.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
A ubiquitously expressed syndecan that is found in all stages of embryonic development and in most adult tissues. Syndecan-4 is found localized to focal adhesion sites in fibronectin-adherent cells and may play a role the process of CELL MIGRATION and CELL PROLIFERATION.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
The profession of writing. Also the identity of the writer as the creator of a literary production.
Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Sequential operating programs and data which instruct the functioning of a digital computer.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
The edible portions of any animal used for food including domestic mammals (the major ones being cattle, swine, and sheep) along with poultry, fish, shellfish, and game.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Ratings of the characteristics of food including flavor, appearance, nutritional content, and the amount of microbial and chemical contamination.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.
Tumors or cancer of the LUNG.
A cell line derived from cultured tumor cells.
Agents that inhibit PROTEIN KINASES.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.
Glypican 5: encoding protein Glypican-5 HTR2A: 5-HT2A receptor INTS6: encoding protein Integrator complex subunit 6 GPALPP1: ... 2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522-8. Bibcode:2004Natur.428..522D. doi: ... encoding protein Family with sequence similarity 155, member A FLT1: Fms related tyrosine kinase 1 (Vascular endothelial growth ... the collaborative consensus coding sequence project (CCDS) takes an extremely conservative strategy. So CCDS's gene number ...
The glypicans found in vertebrates, Drosophila, and C. elegans all have an N-terminal signal sequence. Glypicans are critically ... Furthermore, the amino acid sequence and structure of each glypican is well-conserved between species; all vertebrate glypicans ... Six glypicans have been identified in mammals, and are referred to as GPC1 through GPC6. In Drosophila two glypicans have been ... One additional glypican has been identified in C. elegans. Glypicans seem to play a vital role in developmental morphogenesis, ...
2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522-8. doi:10.1038/nature02379. PMC 2665288 ... The glypicans have been implicated in the control of cell growth and division. Glypican 6 is a putative cell surface coreceptor ... Glypican-6 is a protein that in humans is encoded by the GPC6 gene. The glypicans comprise a family of ... 1999). "Glypican-6, a new member of the glypican family of cell surface heparan sulfate proteoglycans". J. Biol. Chem. 274 (38 ...
2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... and in more recent overviews of potential markers for pancreatic cancer, Glypican 1 is not mentioned. Glypican GRCh38: Ensembl ... Glypican-1 is a protein that in humans is encoded by the GPC1 gene. Cell surface heparan sulfate proteoglycans are composed of ... Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the ...
October 1997). "Analysis of exon/intron structure and 400 kb of genomic sequence surrounding the 5'-promoter and 3'-terminal ... "Entrez Gene: GPC3 glypican 3". Jakubovic BD, Jothy S (April 2007). "Glypican-3: from the mutations of Simpson-Golabi-Behmel ... December 1998). "Glypican 3 and glypican 4 are juxtaposed in Xq26.1". Gene. 225 (1-2): 9-16. doi:10.1016/S0378-1119(98)00549-6 ... Glypican-3 is a protein that, in humans, is encoded by the GPC3 gene. The GPC3 gene is located on human X chromosome (Xq26) ...
2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522-8. doi:10.1038/nature02379. PMC 2665288 ... "Entrez Gene: GPC5 glypican 5". De Cat B, David G (2001). "Developmental roles of the glypicans". Semin. Cell Dev. Biol. 12 (2 ... Glypican-5 is a protein that in humans is encoded by the GPC5 gene. Cell surface heparan sulfate proteoglycans are composed of ... Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the ...
2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... "Role for amplification and expression of glypican-5 in rhabdomyosarcoma". Cancer Res. 67 (1): 57-65. doi:10.1158/0008-5472.CAN- ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
Glypican-3 (GPC3) interacts with both Wnt and Frizzled to form a complex and triggers downstream signaling. It has been ... Sadir R, Forest E, Lortat-Jacob H (May 1998). "The heparan sulfate binding sequence of interferon-gamma increased the on rate ... It has been demonstrated that 3-O-sulfation can enhance the binding of Wnt to the glypican and may play a role in regulating ... Li N, Gao W, Zhang YF, Ho M (November 2018). "Glypicans as Cancer Therapeutic Targets". Trends in Cancer. 4 (11): 741-754. doi: ...
1994). "Isolation and sequencing of cDNAs for proteins with multiple domains of Gly-Xaa-Yaa repeats identify a distinct family ... 2001). "Cell surface glypicans are low-affinity endostatin receptors" (PDF). Mol. Cell. 7 (4): 811-22. doi:10.1016/S1097-2765( ... 2000). "The DNA sequence of human chromosome 21". Nature. 405 (6784): 311-9. Bibcode:2000Natur.405..311H. doi:10.1038/35012518 ...
Protein sequence alignment studies indicate that this lysine is conserved in cytosolic sulfotransferases as well. In addition ... or glypican. Sulfation of heparan sulfate GAGs helps give diversity to cell surface proteins and provides them with a unique ...
The mutations causing this syndrome have been mapped to the glypican 4 (GPC4) gene. This gene is located on the long arm of ... A 2007 study concluded, based on analysis of microsatellite markers (small gene sequences found in common among individuals ...
2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ... Capurro MI, Shi W, Sandal S, Filmus J (2006). "Processing by convertases is not required for glypican-3-induced stimulation of ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...
"Entrez Gene: GPC3 glypican 3". Abdulhady, H; El-Sobky, TA; Elsayed, NS; Sakr, HM (11 June 2018). "Clinical and imaging features ... Opitz JM, Jorde LB (July 27, 2011). "Hamartoma Syndromes, Exome Sequencing, and a Protean Puzzle". The New England Journal of ... which codes for glypican 3 and may play a role in regulating cell division and growth regulation. Macrodystrophia lipomatosa ... "Mutation analysis of the tumor suppressor PTEN and the glypican 3 (GPC3) gene in patients diagnosed with Proteus syndrome". Am ...
"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain ... Sulf2 also upregulated glypican-3, which is commonly overexpressed in HCC, by increasing ERK, AKT activation through enhanced ... Sequence alignment analysis indicates QSsulf1 is homologous with lysosomal N-acetyl glucosamine sulfatases (G6-sulfatases) that ... GPC3 is important in Sulf2-enhanced FGF signaling in vitro, so glypican-3 may mediate its own upregulation through Sulf2. Given ...
In 1989, Zimmermann and Ruoslahti cloned and sequenced the core protein of fibroblast chondroitin sulfate proteoglycan. They ... cell adhesion molecules and members of the syndecan and glypican proteoglycan families. Versican's C-terminal domain interacts ... "Identification of the proteoglycan versican in aorta and smooth muscle cells by DNA sequence analysis, in situ hybridization ... "A fibroblast chondroitin sulfate proteoglycan core protein contains lectin-like and growth factor-like sequences". J. Biol. ...
March 2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proceedings of the National ... Human FAT1 was found to bind glypican-3 (GPC3) and regulate cell migration in liver cancer cells. The human FAT1 cadherin gene ... September 2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". ... January 2021). "Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells". ...
Wang C, Gao W, Feng M, Pastan I, Ho M (May 2017). "Construction of an immunotoxin, HN3-mPE24, targeting glypican-3 for liver ... Stability towards gastric acid and proteases depends on the amino acid sequence. Some species have been shown to be active in ... Gao W, Tang Z, Zhang YF, Feng M, Qian M, Dimitrov DS, Ho M (March 2015). "Immunotoxin targeting glypican-3 regresses liver ... Li N, Fu H, Hewitt SM, Dimitrov DS, Ho M (August 2017). "Therapeutically targeting glypican-2 via single-domain antibody-based ...
Two separate loop sequences, incorporating a total of 12 to 36 amino acids, form the target interaction surface so interaction ... "Development of an Affimer-antibody combined immunological diagnosis kit for glypican-3". Scientific Reports. 7 (1): 9608. doi: ... Affimer proteins display two peptide loops and an N-terminal sequence that can all be randomised to bind to desired target ... Phage display libraries of 1010 randomised potential target interaction sequences are used to screen for Affimer proteins that ...
Sequences that direct AG glycosylation (SO, TO, AO, VO) are called AGP glycomotifs. All AGP protein backbones contain a minimum ... Filmus, Jorge; Capurro, Mariana; Rast, Jonathan (2008). "Glypicans". Genome Biology. 9 (5): 224. doi:10.1186/gb-2008-9-5-224. ... "Classical" AGPs include the GPI-AGPs that consist of a signal peptide at the N-terminus, a PAST-rich sequence of 100-150 aa and ... The 'Hyp contiguity hypothesis' predicts that when O occurs in a non-contiguous manner, for example the sequence 'SOTO', such ...
Genomic sequencing analysis of CAR insertion sites in T cells has been established for better understanding of CAR T cell ... Li N, Fu H, Hewitt SM, Dimitrov DS, Ho M (August 2017). "Therapeutically targeting glypican-2 via single-domain antibody-based ... Hinge sequences are often based on membrane-proximal regions from other immune molecules including IgG, CD8, and CD28. The ... February 2020). "Persistent Polyfunctional Chimeric Antigen Receptor T Cells That Target Glypican 3 Eliminate Orthotopic ...
2000). "The DNA sequence of human chromosome 21". Nature. 405 (6784): 311-9. doi:10.1038/35012518. PMID 10830953.. ... "Cell surface glypicans are low-affinity endostatin receptors" (PDF). Mol. Cell. 7 (4): 811-22. doi:10.1016/S1097-2765(01)00225 ... "Isolation and sequencing of cDNAs for proteins with multiple domains of Gly-Xaa-Yaa repeats identify a distinct family of ...
Rabbit polyclonal Glypican 4 antibody. Validated in IHC, ICC/IF and tested in Human. Immunogen corresponding to recombinant ... Recombinant fragment corresponding to Human Glypican 4 aa 473-533.. Sequence: ... Primary - Rabbit Anti-Glypican 4 antibody (ab246973) IHC-P, ICC/IF Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718) IHC-P ... PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for Glypican 4 (green) using ab246973 at 4μg/ml in ICC/IF. ...
Rabbit recombinant monoclonal Glypican 3 antibody [SP86] validated for IHC and tested in Human. Immunogen corresponding to ... and a next-generation sequencing platform (NGS-RabMAb®) ... Anti-Glypican 3 antibody [SP86], prediluted. See all Glypican 3 ... Prediluted ab95364 staining Glypican 3 in formalin-fixed, paraffin-embedded Human liver cancer tissue by Immunohistochemistry. ... Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glypican 3 antibody [SP86] - C-terminal, prediluted ( ...
Glypican-5 appeared overexpressed in high-grade tumors with epithelial differentiation, and not in those that displayed a ... Whilst elevated glypican-1 expression has been documented in different tumours, the down-regulation in high grade tumors ... In contrast, normal neuroendocrine cells were positive for glypican 1, displaying intense staining in cytoplasm and membrane. ... In contrast, normal neuroendocrine cells were positive for glypican 1, displaying intense staining in cytoplasm and membrane. ...
Glypicans: proteoglycans with a surprise Jorge Filmus et al. * Heparan sulfate: growth control with a restricted sequence menu ... Heparin sequencing brings structure to the function of complex oligosaccharides. Proc Natl Acad Sci USA 2000. 97:10301-10303. ... The development of novel experimental strategies for decoding the sequence of heparan sulfate (8-10), together with the ... Glypicans. In Proteoglycans: structure, biology and molecular interactions. R.V. Iozzo, editor. Marcel Dekker Inc. New York, ...
The prepared DNA sequence unitary fragment may be delivered to endothelial cells in-situ, both under in-vivo and/or in-vitro ... The tangible means include a prepared DNA sequence fragment having separate and individual DNA sequenced portions coding for an ... The glypican-syndecan-4 cytoplasmic domain (G1-S4c) construct was created by replacing C-terminal sequence of rat glypican-1 ... Syndecan/glypican chimeras were created via PCR mutagenesis; cloned into the pCDNA3; sequenced; and shuttled into the MSCV2.2 ...
2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522-8. doi:10.1038/nature02379. PMC 2665288 ... The glypicans have been implicated in the control of cell growth and division. Glypican 6 is a putative cell surface coreceptor ... Glypican-6 is a protein that in humans is encoded by the GPC6 gene. The glypicans comprise a family of ... 1999). "Glypican-6, a new member of the glypican family of cell surface heparan sulfate proteoglycans". J. Biol. Chem. 274 (38 ...
2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522-8. doi:10.1038/nature02379. PMC 2665288 ... "Entrez Gene: GPC5 glypican 5". De Cat B, David G (2001). "Developmental roles of the glypicans". Semin. Cell Dev. Biol. 12 (2 ... Glypican-5 is a protein that in humans is encoded by the GPC5 gene. Cell surface heparan sulfate proteoglycans are composed of ... Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the ...
Rabbit Polyclonal Glypican 5 antibody [N2C3]. Validated in WB. Tested in Human., Find more about GeneTex products - ... Recombinant protein encompassing a sequence within the center region of human Glypican 5. The exact sequence is proprietary. ... glypican 5 Cellular Localization Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-5: Secreted ... Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the ...
N-sulfation of glucosamine residues, catalyzed by Sfl, is the first step in a sequence of HS modifications, and is essential ... Glypicans regulate FSC maintenance. Since FSCs express both glypicans, we examined the functions of dally and dlp in ... Model for regulation of FSC competition by glypicans. Glypicans regulate signaling dosage of multiple signaling pathways ... Roles of glypicans in Wg signaling in FSCs. (A-B) fz3-RFP reporter assay in control (A and A) and 109-30,sfl RNAi (B and B) ...
GE100003, scramble sequence in pCas-Guide vector. CRISPR Manual:. CRISPR/CAS9 Genome Editing Manual. ... Background of Glypican-3 / GPC3 antibody. Kit Component:. - KN307156G1, Gpc3 gRNA vector 1 in pCas-Guide vector. - KN307156G2, ... Western Blot of HepG2 cell lysate with anti-Glypican-3 Antibody Cat.-No AM12128PU (Clone SP86).. *AM12128PU-N ... Flow cytometric analysis of rabbit anti-Glypican-3 (SP86) antibody in HEPG2 (green) compare to negative control of rabbit IgG ( ...
glypican-1. NGS:. next-generation sequencing. NSCLC:. non-small cell lung cancer ... One approach involves whole-exome sequencing, which can be adopted for sequencing of cfDNA for the identification of clinically ... is an approach employing genome-wide bisulfite sequencing of plasma DNA and methylation deconvolution of the sequencing data to ... The sequencing coverage differed from unexpressed genes, which are densely packed by nucleosomes [76] (Fig. 2b). In fact, ...
Interacts with glypican GPC3 (By similarity).. By similarity. Manual assertion inferred from sequence similarity toi ... Sequence databases. Select the link destinations:. EMBL nucleotide sequence database. More...EMBLi. GenBank nucleotide sequence ... help/sequence_processing target=_top>More...,/a>,/p>Sequence processingi: The displayed sequence is further processed into a ... Sequence conflicts are usually of unknown origin.,p>,a href=/help/conflict target=_top>More...,/a>,/p>Sequence conflicti. ...
Glypican 4, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... glypican 4,K glypican,60kDa,putative extracellular proteoglycan,ubiquitously expressed,predominantly in fetal lung and kidney, ... The DNA sequence of the human X chromosome. (PMID: 15772651) Ross MT … Bentley DR (Nature 2005) 3 4 58 ... Suggested Antigen Peptide Sequences for GPC4 Gene. GenScript: Design optimal peptide antigens:. *cDNA FLJ51457, highly similar ...
Organization, 5-flanking sequence and promoter activity of the rat GPC1 gene. Asundi, V.K., Keister, B.F., Carey, D.J. Gene ( ... Characterization of glypican-5 and chromosomal localization of human GPC5, a new member of the glypican gene family. Veugelers ... Glypican 3 and glypican 4 are juxtaposed in Xq26.1. Huber, R., Mazzarella, R., Chen, C.N., Chen, E., Ireland, M., Lindsay, S., ... Characterization of glypican-5 and chromosomal localization of human GPC5, a new member of the glypican gene family [24]. ...
Sequencing and primer design rolled into one. Just give us your clone and a reference sequence, and well do the rest. Single- ... 2-Fluorine-CTP (2-F-CTP) from EPICENTRE BiotechnologiesGoat Anti-Human Glypican 2 Polyclonal Antibody, Biotin Conjugated from R ... Sequencing and primer design rolled into one. Just give us your clone and a reference sequence, and well do the rest. Single- ... Whole-Genome Shotgun Sequencing from Seqwright. 6. FDA-Submission Quality DNA Sequencing from Seqwright. 7. QuickLane ...
Glypican 5 antibody LS-C321718 is an unconjugated rabbit polyclonal antibody to Glypican 5 (GPC5) (aa56-84) from human and ... Polyclonal GPC5 / Glypican 5 Antibody (aa56‑84, IHC, WB) LS‑C321718 Polyclonal GPC5 / Glypican 5 Antibody (aa56‑84, IHC, WB) LS ... Glypican 5 antibody LS-C321718 is an unconjugated rabbit polyclonal antibody to Glypican 5 (GPC5) (aa56-84) from human and ...
Lentiviral silencing of glypican 6, encoded at this locus, led to the up-regulation of proinflammatory markers in a ... Oligonucleotide Array Sequence Analysis * Phenotype * Polymorphism, Single Nucleotide* * Risk Assessment * Risk Factors ...
Suggested Antigen Peptide Sequences for OAF Gene. GenScript: Design optimal peptide antigens:. *HCV NS5A-transactivated protein ... Mass spectrometry-based proteomics revealed Glypican-1 as a novel ADAM17 substrate. (PMID: 27576135) Kawahara R … Paes Leme AF ... Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PMID: 12477932) Strausberg RL ... fRNAdb sequence ontologies and piRNA Summary for OAF Gene ...
3-1). Apart from the previously validated shRNA sequences, 12 shRNA sequences screened out from the current study were further ... Given that Nrxn1α instructs PTPσ to induce activation of glypican/LRRTM4 complexes (Ko et al., 2015), and is also further ... 2008) Polarized targeting of neurexins to synapses is regulated by their C-terminal sequences. J Neurosci 28:12969-12981. doi: ... The detailed oligonucleotides sequences of shRNAs used in the current study and their KD efficacies are presented in Table 1, ...
The primary sequence of Dof shares similar domains with two vertebrate proteins, BCAP and BANK [55]. These proteins regulate B- ... Dally and Dlp are glypicans, which are attached to the membrane via GPI membrane anchor, while Sdc is a transmembrane ... However, amino acid sequence motifs in the Dof adaptor imply that signal transmission through these conserved signal ... The intracellular kinase domain of Htl and Btl shows high sequence homology (over 75% amino acid identity), but they differ in ...
Next-generation sequencing of circulating tumor DNA for early cancer detection. Cell 2017;168:571-574. doi:10.1016/j.cell. ... Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. 2015;523:177-182. doi:10.1038/nature14581 ... Analytical and clinical validation of a digital sequencing panel for quantitative, highly accurate evaluation of cell-free ... but as genetic sequencing and computerized analysis improve, we are likely to see these methods being studied in clinical ...
... glypican 6) is implicated in bone growth [51]. The FAM3C gene, or Family With Sequence Similarity 3, Member C was found ... Boning up on glypicans-opportunities for new insights into bone biology. Cell Biochem Funct. 2013;31(2):91-114. doi: 10.1002/ ... Mapping and exome sequencing identifies a mutation in the IARS gene as the cause of hereditary perinatal weak calf syndrome. ... Genome-wide association study reveals five nucleotide sequence variants for carcass traits in beef cattle. Anim Genet. 2011;42( ...
UTRs contain putative miR-205 target sequences (44, 45). It has been shown that CRIPTO1 activates SRC upon binding to glypican- ... A Nodal- and ALK4-independent signaling pathway activated by Cripto-1 through Glypican-1 and c-Src. Cancer Res. 2003;63(6):1192 ... The latter 3 biological responses to CRIPTO1 probably occur through a GLYPICAN-1/SRC pathway that activates MAPK and PI3K/Akt ... a pseudogene that shares significant sequence homology with CRIPTO1 (46). Interestingly, CRIPTO1 expression was clearly ...
Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Antibody, Recombinant Mouse Monoclonal Antibody validated in IHC-P, IF ... Custom peptide services include long peptides (>100mer), cyclic peptides, difficult sequences, fluorescent labels, and ... Anti-Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Antibody is for research use only and not for use in diagnostic or ... Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti ...
GPC3 and GPC4 lay tandem in sequence within the Xq26 locus.. * The glypican‐3 protein mediates embryonic and cell‐specific ... Huber R, Mazzarella R and Chen CN (1998) Glypican 3 and glypican 4 are juxtaposed in Xq26.1. Gene 225: 9-16. ... to a truncated protein or nonfunctional glypican‐3 or glypican‐4 protein. ... 1998) GPC4, the gene for human K‐glypican, flanks GPC3 on xq26: deletion of the GPC3‐GPC4 gene cluster in one family with ...
family with sequence similarity 3, member A. GPC3. 2719. GPC3. glypican 3. ... of MBD depletion using microarray-based expression analysis in conjunction with extensive bisulfite genomic sequencing and ...
The cis-element sequences cloned into the reporter vector were: m4S1 [a known Su(H) target site from E(spl)-C], caccgagt ... Although it is clear in the Drosophila ovarian niche that glypican is pro-BMP signaling, glypicans role in developmental ... and how glypican expression is regulated. We have previously reported that Dally, one of the glypican members in Drosophila, is ... where glypicans have been intensively studied, glypican Dally or Dally-like is necessary for the diffusion, stability and/or ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The PCR products were cloned (TA cloning kit, Invitrogen) and sequenced using the PRISMTM dye terminator cycle sequencing core ... 1997) OCI-5/rat glypican-3 binds to fibroblast growth factor-2 but not to insulin-like growth factor-2. J Biol Chem 272:7574- ... and sequenced with EX2 A. The comparison of the sequences from the patient (A) with normal (B) shows a 13 bp deletion in exon 2 ... Sequence analysis of the normal and mutant alleles. Both normal and mutant alleles were PCR amplified with EX2 A and EX2 B, ...
On average, about 21.6 million sequencing reads and 10.6 million aligned reads were obtained for samples sequenced on each lane ... our ability to sequence 17K genes at sequence depth of ,10 tags per gene was quite good. We believe that this sequence depth ... 2003) Glypican-3, overexpressed specifically in human hepatocellular carcinoma, is a novel tumor marker. Biochem Biophys Res ... was not significantly increased for sequenced samples on 2 or 3 sequencing lanes, compared with those on one sequencing lane ( ...
  • GPC3 and GPC4 lay tandem in sequence within the Xq26 locus. (els.net)
  • Point mutations or microdeletions in GPC3 or duplication of GPC4 result in a premature termination codon and will be translated to a truncated protein or nonfunctional glypican‐3 or glypican‐4 protein. (els.net)
  • 2000) Mutational analysis of the GPC3/GPC4 glypican gene cluster on Xq26 in patients with Simpson‐Golabi‐Behmel syndrome: identification of loss‐of‐function mutations in the GPC3 gene. (els.net)
  • 1998) GPC4, the gene for human K‐glypican, flanks GPC3 on xq26: deletion of the GPC3‐GPC4 gene cluster in one family with Simpson‐Golabi‐Behmel syndrome. (els.net)
  • Deletions in the heparan sulphate proteoglycan encoding glypican 3 (GPC3) gene have recently been documented in several Simpson-Golabi-Behmel syndrome (SGBS) families. (bmj.com)
  • Recently, a membrane associated heparan sulphate proteoglycan, glypican 3 (GPC3), has been identified as the SGBS gene. (bmj.com)
  • 3 GPC3 is a member of the glypican related integral membrane proteoglycans (GRIPS) which are linked to the cell surface via glycosyl phosphatidylinositol and modulate the interaction between growth factors and receptors. (bmj.com)
  • 3-5 GPC3 encodes a protein of 580 amino acids and, despite a comparatively small 2.3 kb transcript, spans more than 500 kb of genomic sequence. (bmj.com)
  • Glypican-3(GPC3) has been implicated in tumor development and progression for several years. (biomedcentral.com)
  • Glypican-3 (GPC3), a member of the glypican family of heparan-sulfate proteoglycans (HSPGs), is bound to the plasma membrane through a glycosyl phosphatidylinositol (GPI) anchor. (biomedcentral.com)
  • So far, six members (Glypican-1/GPC1, Glypican-2/GPC2, Glypican-3/GPC3, Glypican-4/GPC4, Glypican-5/GPC5, Glypican-6/GPC6) of this family are known in vertebrates. (sinobiological.com)
  • Codrituzumab is the first-in-class, recombinant, humanized monoclonal antibody which binds to glypican-3 (GPC3). (guidetopharmacology.org)
  • As a cell surface proteoglycan anchored by glycosyl-phosphatidylinositol, Glypican-3 (GPC3) is reported to be highly expressed in hepatocellular carcinoma (HCC) and to promote cell proliferation and tumorigenesis through activating Wnt/β-catenin signalling. (portlandpress.com)
  • Comparison of differentially expressed genes by RNA-seq revealed downregulation of hepatocyte metabolism genes and re-expression of hepatoblast genes, including Glypican-3 (Gpc3). (ubc.ca)
  • Among the differentially expressed genes, glypican-3 (GPC3), a member of glypican family, was markedly elevated in CD90(+)CSCs compared to CD90(+)NTSCs. (nih.gov)
  • There are currently no references for Glypican 5 antibody [N2C3] (GTX104125) . (genetex.com)
  • Human liver cancer stained with anti-Glypican-3 Antibody Cat. (acris-antibodies.com)
  • Western Blot of HepG2 cell lysate with anti-Glypican-3 Antibody Cat. (acris-antibodies.com)
  • Flow Cytometric analysis of Rabbit anti-Glypican-3 Antibody Cat. (acris-antibodies.com)
  • Formalin-Fixed, Paraffin-Embedded Human hepatocellular carcinoma stained with Glypican-3 antibody Cat. (acris-antibodies.com)
  • Glypican 5 antibody LS-C321718 is an unconjugated rabbit polyclonal antibody to Glypican 5 (GPC5) (aa56-84) from human and mouse. (lsbio.com)
  • Subsequently, targeted quantitation by mass spectrometry can be used to refine candidates of interest, and a bioinformatic visualization tool is key to mapping experimental data to candidate protein sequences for the purpose of epitope selection during the antibody development phase. (nih.gov)
  • Peptide sequence information for this antibody is available from its IMGT/mAb-DB record. (guidetopharmacology.org)
  • The following antibody was used in this experiment: Glypican 1 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-86043, RRID AB_2802844. (thermofisher.com)
  • Find the Glypican 3 antibody that fits your needs. (thermofisher.com)
  • Measles sequences mimicking intestinal proteins are frequent targets of antibody responses in patients with CD, but this reactivity lacks disease specificity and does not initiate cross-reactive responses to intestinal mimics. (biomedcentral.com)
  • Glypican-6 is a protein that in humans is encoded by the GPC6 gene. (wikipedia.org)
  • Glypican-5 is a protein that in humans is encoded by the GPC5 gene. (wikipedia.org)
  • GPC4 (Glypican 4) is a Protein Coding gene. (genecards.org)
  • Identical with the Gene Bank Ref. ID sequence except for a point mutation of 464C/T resulting in an amino acid Ala substitution by Val. (sinobiological.com)
  • The properties of Glypican-5 make it an attractive target for therapeutic intervention in rhabdomyosarcomas and other tumors that amplify and/or overexpress its gene. (sinobiological.com)
  • and GPC6, a gene that encodes glypican-6, which regulates cell growth and division. (fredhutch.org)
  • Whole-exome sequencing of the Australian family that defined Keipert syndrome (nasodigitoacoustic syndrome) identified a hemizygous truncating variant in the gene encoding glypican 4 (GPC4). (salk.edu)
  • Hotspot mutations in TP53 gene were analyzed by sequencing. (iiarjournals.org)
  • One of the alternative versions of a gene (i.e variation in DNA sequence) that occurs at a specific chromosomal locus (site). (ifopa.org)
  • Whole genome methylation sequencing reveals distinct impact of differential methylations on gene transcription in prostate cancer. (upmc.edu)
  • Sequencing of the p63 gene reveals a new heterozygous frameshift mutation, 1787delG, in exon 14. (biomedsearch.com)
  • In addition, gene organization is different, as eukaryotic genes are interrupted by intronic sequences. (springer.com)
  • Gene expression profiling of liver cancer stem cells by RNA-sequencing. (nih.gov)
  • In this study, we employed a more sensitive RNA-sequencing (RNA-Seq) to compare the gene expression profiling of CD90(+) cells sorted from tumor (CD90(+)CSCs) with parallel non-tumorous liver tissues (CD90(+)NTSCs) and elucidate the roles of putative target genes in hepatocarcinogenesis. (nih.gov)
  • See reference mRNA sequence for the Gpc4 gene (XM_006257580.3). (nih.gov)
  • See the other reference sequence protein isoform for the Gpc4 gene (NP_001014130.1). (nih.gov)
  • A complete sequence of the X. laevis genome is an essential resource for accurate identification of peptides for mass-spec analyses, for cloning of an ORFeome, for identifying evolutionarily conserved regulatory regions, and for design of morpholino-oligonucleotides for gene knockdowns. (marcottelab.org)
  • Gene expression studies in Drosophila suggest that Mmps cooperate with the heparan sulfate proteoglycan (HSPG) glypican co-receptor Dally-like protein (Dlp) to restrict trans-synaptic Wnt signaling and that synaptogenic defects in the fly model of FXS are alleviated by either inhibition of Mmp or genetic reduction of Dlp. (sciencemag.org)
  • Partly in response to our findings, Jeff Murray's group sequenced the coding region GRHL3 gene in Van der Woude patients lacking IRF6 mutations and found 8 different coding mutations in GRHL3. (uiowa.edu)
  • We clarified 7 cardinal gene families, including the MAPK, STE20 group kinase, Rho small GTPase, STAT, Glypican, Fz and Wnt gene families, as well as gene homologs or orthologs for known PCP/CE signaling components with their phylogenetic nature. (edu.sa)
  • A complete sequence of the X. laevis genome is an essential resource for accurate identification of peptides for mass-spec analyses, for cloning of an ORFeome, for identifying evolutionarily conserved regulatory region, and for design of morpholino-oligonucleotides for gene knockdowns. (yeastnet.org)
  • Orthologous to human GPC1 (glypican 1). (zfin.org)
  • Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. (cdc.gov)
  • Overall, we have shown that pathogenic variants in GPC4 cause a loss of function that results in Keipert syndrome, making GPC4 the third human glypican to be linked to a genetic syndrome. (salk.edu)
  • Glypican 4 (Gpc4) is a heparan sulfate proteoglycan that regulates glutamatergic synapse formation and function in the developing brain. (salk.edu)
  • Glypican 4, also known as GPC4, is part of a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) which take part in the control of cell division and growth regulation. (prospecbio.com)
  • Recent advances in sequencing and in mapping RNA reads to reference genomes have revealed that thousands of genes express also covalently closed circular RNAs. (springer.com)
  • Then, we used the RNA sequencing data from the embryos and larvae of Ciona savignyi to identify ECM genes and acquire their expression patterns. (biologists.org)
  • The selected BACs include ~70 genes of interest (Shroom3, Wnt5a, Glypican-4, Noggin, Gremlin, Pax6, Formin, etc., as initially identified by the group of Jan-Fang Cheng via probing the CHORI-219 library), as well as 10 BACs that have already been sequenced by the DOE Joint Genome Institute / HudsonAlpha Genome Sequencing Center to serve as positive controls for the sequencing and assembly pipeline. (marcottelab.org)
  • Finally, we found that the npr-1 and npr-3 mutants have similar behavioral patterns that were not predictable by sequence homology, proving that our data-driven approach can reveal the functions of genes that have not yet been characterized. (biomedcentral.com)
  • All of these studies have expanded our understanding of the genetics behind CL/P, either by discovering new loci in human patients and testing them in Danio rerio, or from finding downstream targets of Irf6 in zebrafish, sequencing human patients for mutations in those genes, and then testing the functional changes of those variants. (uiowa.edu)
  • In mom1 mutants, although TGS is released at transposons, repetitive sequences and transgenes, silencing release occurs in this case without major alterations in DNA or histone modification, as is the case for the mutation of genes necessary for maintenance of cytosine and histone methylation [17] - [21] . (prolekare.cz)
  • The selected BACs include ~70 genes of interest (Shroom3, Wnt5a, Glypican-4, Noggin, Gremlin, Pax6, Formin, etc.), as well as 10 BACs that have already been sequenced by HudsonAlpha to serve as positive controls for the sequencing and assembly pipeline. (yeastnet.org)
  • Dr. Manak discovered a de novo single copy deletion encompassing Glypican 5 (GPC5) and part of Glypican 6 (GPC6) in a spina bifida patient. (uiowa.edu)
  • The PCR products were cloned (TA cloning kit, Invitrogen) and sequenced using the PRISM TM dye terminator cycle sequencing core kit with AmpliTaq @ DNA polymerase, FS (ABI). (bmj.com)
  • The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans. (wikipedia.org)
  • Here, we show that two Drosophila members of the glypican family of heparan sulfate proteoglycans (HSPGs), Dally and Dally-like (Dlp), differentially regulate follicle stem cell (FSC) maintenance and competitiveness for niche occupancy. (genetics.org)
  • The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. (sinobiological.com)
  • Glypicans are a family of cell-surface heparan sulfate proteoglycans that regulate growth-factor signaling during development and are thought to play a role in the regulation of morphogenesis. (salk.edu)
  • Blocking or removal of cell surface proteoglycans evidenced that syndecans play a more important role than glypicans. (springer.com)
  • Glypicans Glypicans unlike the syndecans are not transmembrane heparan sulfate proteoglycans, but attached to the cell surface through a GPI lipid anchor. (mildredveitia.com)
  • Glypican-5 (GPC5), is a cell membrane protein which belongs to the glypican family. (sinobiological.com)
  • Cloning and sequencing of a fragment from the Drosophila cDNA library revealed it to be a glypican, a heparan sulfate proteoglycan (HSPG) attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. (lu.se)
  • Glypican 6 is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. (wikipedia.org)
  • 1994). "Isolation and sequencing of cDNAs for proteins with multiple domains of Gly-Xaa-Yaa repeats identify a distinct family of collagenous proteins" . (wikipedia.org)
  • Heparan sulfate is attached to transmembrane or glycolipid-anchored membrane proteins (syndecans and glypicans respectively). (galenlabsupplies.com)
  • The BLAST 2p program was used to identify amino acid sequence similarities between five measles virus and 56 intestinal proteins. (biomedcentral.com)
  • HSPG core proteins Syndecan (SDN-1) and Glypican (LON-2) and the HS modifying enzymes in the dorsal guidance of D-type motor axons, a process controlled mainly by the conserved axon guidance molecule UNC-6/Netrin. (queensu.ca)
  • H) Sequence alignment of Ttv, Sotv and Botv proteins. (biologists.org)
  • Whilst elevated glypican 1 expression has been documented in different tumors, the downregulation in high-grade tumors observed in this work suggests that this proteoglycan could be involved in cancer development in a more complex and context-dependent manner than previously thought. (frontiersin.org)
  • Expression of the cell surface proteoglycan glypican-5 is developmentally regulated in kidney, limb, and brain" (PDF). (wikipedia.org)
  • The prepared DNA sequence unitary fragment may be delivered to endothelial cells in-situ, both under in-vivo and/or in-vitro conditions, using suitable expression vectors including plasmids and viruses. (google.es)
  • This requires clarification on how the spatial patterns of glypican relate to the distribution of signaling molecules in different cellular contexts, and how glypican expression is regulated. (biologists.org)
  • and studied the functional consequences of MBD depletion using microarray-based expression analysis in conjunction with extensive bisulfite genomic sequencing and chromatin immunoprecipitation. (broadinstitute.org)
  • With the advance of the next-generation sequencing technologies, RNA-seq has become a useful tool in defining the transcriptomes of cells with the advantage of analyzing expression at exon levels as well as delineating novel splicing variants [10] , [11] . (plos.org)
  • Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (sinobiological.com)
  • 2007) Role for amplification and expression of glypican-5 in rhabdomyosarcoma. (sinobiological.com)
  • Therefore, by microarray analysis, we have investigated the expression profiles of more than 50,000 human lncRNAs in blood samples from PsA patients and healthy controls using Human Clariom D Affymetrix chips, suitable to detect rare and low-expressing transcripts otherwise unnoticed by common sequencing methodologies. (frontiersin.org)
  • Materials and Methods: Expression of CK19, Glypican-3 and β-catenin was assessed in clinical samples by immunohistochemistry (IHC). (iiarjournals.org)
  • Dally-like protein, a new Drosophila glypican with expression overlapping with wingless.Khare N. and Baumgartner S. (2000). (lu.se)
  • These antibodies have been verified by Relative expression to confirm specificity to Glypican 3. (thermofisher.com)
  • The extracellular domain of glypican was inserted into the expression vector APtag-2, immediately upstream of the AP coding sequence (Figure 1). (uci.edu)
  • Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures. (nih.gov)
  • Expression of glypican-4 in haematopoietic-progenitor and bone-marrow-stromal cells. (nih.gov)
  • Tissue factor expression in the metaplasia-adenoma-carcinoma sequence of gastric cancer in a European population. (ebscohost.com)
  • We performed deep sequencing to investigate the expression of small RNAs in plasma EVs from lung adenocarcinoma (ADC) patients, lung squamous cell carcinoma (SQCC) patients, and healthy controls. (biomedcentral.com)
  • These include four syndecans, six glypicans, and one each of perlecan, agrin, and the hybrid HSPG/collagen type XVIII. (jci.org)
  • Recombinant fragment corresponding to Human Glypican 4 aa 473-533. (abcam.com)
  • Recombinant protein encompassing a sequence within the center region of human Glypican 5. (genetex.com)
  • Glypican-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ala19-Ser529) containing 521 amino acids including a 10 aa His tag at N-terminus. (prospecbio.com)
  • Our Glypican 3 polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Rabbit, Mouse and Sheep. (thermofisher.com)
  • Song H and Filmus J (2002) The role of glypicans in mammalian development. (els.net)
  • Dally-like and Drosophila CNS: A role of glypicans in the developing nervous system.Khare N., Waldrip W. R., Baumgartner S., Selleck S. B. (Manuscript). (lu.se)
  • HSPGs consist of a protein core (such as syndecans and glypicans) to which heparan sulfate glycosaminoglycan (HS GAG) chains are attached. (biologists.org)
  • Although the function of each of the glypicans is not completely understood, each of the glypicans is differentially expressed during development. (mildredveitia.com)
  • Opens the Genome Data Viewer, NCBI's genome browser, at the genomic location at which this sequence is annotated. (nih.gov)
  • In addition, we are generating several mate pair libraries of different sizes from genomic DNA from J strain frogs (obtained from Jacques Robert via Mustafa Khokha ), sequencing each to multiple-fold coverage of the genome. (yeastnet.org)
  • The most interesting finding to come out of our DNA-sequencing project was the discovery of three aggressive tumor types that had 10 times the number of mutations compared to the other advanced prostate cancers we studied," said Dr. Peter Nelson, corresponding author along with Dr. Jay Shendure. (fredhutch.org)
  • This method is more efficient and cost-effective than whole-genome sequencing because it zeroes in on just 1 percent of the human genome-the exome-a highly functional region that harbors the majority of disease-causing mutations. (fredhutch.org)
  • Synthetic peptide derived from the C terminal of Human Glypican 3. (abcam.com)
  • Prediluted ab95364 staining Glypican 3 in formalin-fixed, paraffin-embedded Human liver cancer tissue by Immunohistochemistry. (abcam.com)
  • Human Glypican 2 Biotinylated Affinity Purified PAb. (bio-medicine.org)
  • Combining yeast and fruit fly genetics, we illustrate that Dally is under the transcriptional control of Notch signaling via the transcription factor Su(H). Further, we assayed human glypicans and disease-associated variants in Drosophila ovary, which can serve as an effective system to evaluate the structure-function relationship of human homologs. (biologists.org)
  • Full length Clone DNA of Human glypican 5. (sinobiological.com)
  • These antibodies target Glypican 3 in Human, Mouse and Rat samples. (thermofisher.com)
  • 1hjn is a 1 chain structure with sequence from Human . (proteopedia.org)
  • The consensus coding sequences of human breast and colorectal cancers. (expasy.org)
  • To this end, we used protein database programs where the identification of measles/intestinal homologies was followed by construction of the peptidyl sequences spanning the homologous viral/human sequences. (biomedcentral.com)
  • Finally, the analysis revealed a number of sequencing errors in the chimpanzee and human genome sequences and further illustrated that they could be corrected without resequencing. (hindawi.com)
  • Peptides 15mer overlapping peptide swimming pools spanning AFP and glypican-3 protein sequences were synthesized (Genscript USA Inc, Piscataway NJ). (bx-912.com)
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • We have previously reported that Dally, one of the glypican members in Drosophila , is required in the niche for the maintenance of germline stem cells (GSCs) via short-range BMP signaling in ovary. (biologists.org)
  • F) Structures of Drosophila EXTs, Ttv, Sotv and Botv deduced from genome sequence. (biologists.org)
  • The development of novel experimental strategies for decoding the sequence of heparan sulfate ( 8 - 10 ), together with the availability of antibodies specific for heparan sulfate epitopes ( 11 ), holds promise for deciphering the functional specificity of heparan sulfate in tissues. (jci.org)
  • Antibodies that detect Glypican 3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Flow Cytometry and ELISA. (thermofisher.com)
  • Choose from 1 of 18 Glypican 3 antibodies, which have been validated in experiments with 4 publications and 45 images featured in our data gallery. (thermofisher.com)
  • Based on advances in genome and RNA sequencing and sparked by a publication by Salzman et al. (springer.com)
  • With rapid advances in genome sequencing and editing technologies, systematic and quantitative analysis of animal behavior is expected to be another key to facilitating data-driven behavioral genetics. (biomedcentral.com)
  • In contrast, normal neuroendocrine cells were positive for glypican 1, displaying intense staining in cytoplasm and membrane. (frontiersin.org)
  • Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. (wikipedia.org)
  • The cell-associated HSPGs include four integral membrane syndecans and six glycosyl-phosphatidylinositol-anchored (GPI-anchored) glypicans. (jci.org)
  • The collection, classification, storage, and analysis of biochemical and biological information using computers, especially as applied to molecular genetics and genome sequencing. (ifopa.org)
  • A systematic study of normalization methods for Infinium 450K methylation data using whole-genome bisulfite sequencing data. (upmc.edu)
  • Find regions of similarity between this sequence and other sequences using BLAST. (nih.gov)
  • The tangible means include a prepared DNA sequence fragment having separate and individual DNA sequenced portions coding for an heparan sulfate binding extracellular domain, a central transmembrane domain, and a cytoplasmic domain coding for the syndecan-4 polypeptide. (google.es)
  • Unlike the syndecans which can make direct contact with internal cytoskeletal components to activate signal transduction pathways, glypican activation of signaling pathways must be indirect and involve other transmembrane molecules, since glypican does not contain a transmembrane domain. (mildredveitia.com)
  • Lentiviral silencing of glypican 6, encoded at this locus, led to the up-regulation of proinflammatory markers in a cholangiocyte cell line. (nih.gov)
  • Sequencing and primer design rolled into one. (bio-medicine.org)
  • The primer used for sequencing was EX2 A. All DNA sequencing was performed on an Applied Biosystems 373A automated DNA sequencer. (bmj.com)
  • Lineage analyses of glypican mutant FSC clones showed that dally is essential for normal FSC maintenance. (genetics.org)
  • Furthermore, based on genetic interactions with the DPP-binding protein Crossveinless 2 and biochemical interactions with the glypican Dally-like, we propose that LTL acts in the extracellular space where it completes a novel auto-regulatory loop that modulates BMP activity. (biologists.org)
  • Using the Ciona complete genome sequence and EST sequence data, we present here an initial and exhaustive search in non-vertebrate chordates, Ciona intestinalis for the family members as well as homologs or orthologs that are involved in PCP/CE signaling cascades. (edu.sa)
  • Synthesis and processing of glypican during differentiation of skeletal muscle cells. (mendeley.com)
  • Glypican-1 is found in the brain, kidneys, skeletal muscle, and several other tissues (82-84). (mildredveitia.com)
  • The glypicans have been implicated in the control of cell growth and division. (wikipedia.org)
  • The glypican‐3 protein mediates embryonic and cell‐specific tissue formation and development by regulating growth factors. (els.net)
  • Extracellular glypicans play pivotal roles in organogenesis, stem cell maintenance and cancer development. (biologists.org)
  • However, the regulatory mechanism of glypican pattern in the ovarian stem cell niche remains elusive. (biologists.org)
  • Glypican-5 is over-expressed in lymphoma cell lines that had shown amplification. (sinobiological.com)
  • We look at the effects of cell-type dependent glycanation on the binding affinities of the PG glypican against three common extracellular molecules: Type I collagen, basic fibroblast growth factor (bFGF), and laminin-1. (uci.edu)
  • The glypicans contain an N-terminus signal sequence, followed by a globular domain containing multiple cysteine residues, a glycosaminoglycan attachment domain, and a C-terminus that results in the formation of the GPI anchor to the cell surface. (mildredveitia.com)
  • Studies using lineage tracing and deep sequencing could have implications for the cancer stem-cell model and may help to determine the extent to which it accounts for therapy resistance and disease progression. (queensu.ca)
  • We will present the biomarker analysis of a global phase II clinical trial with an immunotherapy targeting glypican-3, a well-known prognostic factor in HCC. (selectbiosciences.com)
  • Here, we utilized genotyping sequencing data obtained via genome reduction on a sequencing platform to detect 100,114 single nucleotide polymorphisms and perform further biological analysis and functional annotation. (g3journal.org)
  • Paine‐Saunders S, Viviano BL, Zupicich J, Skarnes WC and Saunders S (2000) Glypican‐3 controls cellular responses to Bmp4 in limb patterning and skeletal development. (els.net)
  • The position of the amino-acid change on the UniProtKB canonical protein sequence. (expasy.org)
  • The multiple alignment of the region surrounding the variant against various orthologous sequences. (expasy.org)