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Plants, Genetically Modified
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Islands lying between southeastern North America and northern South America, enclosing the Caribbean Sea. They comprise the Greater Antilles (CUBA; DOMINICAN REPUBLIC; HAITI; JAMAICA; and PUERTO RICO), the Lesser Antilles (ANTIGUA AND BARBUDA and the other Leeward Islands, BARBADOS; MARTINIQUE and the other Windward Islands, NETHERLANDS ANTILLES; VIRGIN ISLANDS OF THE UNITED STATES, BRITISH VIRGINI ISLANDS, and the islands north of Venezuela which include TRINIDAD AND TOBAGO), and the BAHAMAS. (From Webster's New Geographical Dictionary, 1988, p1330)
A class of dibenzylbutane derivatives which occurs in higher plants and in fluids (bile, serum, urine, etc.) in man and other animals. These compounds, which have a potential anti-cancer role, can be synthesized in vitro by human fecal flora. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
An island in the Lesser Antilles, one of the Windward Islands. Its capital is Fort-de-France. It was discovered by Columbus in 1502 and from its settlement in 1635 by the French it passed into and out of Dutch and British hands. It was made a French overseas department in 1946. One account of the name tells of native women on the shore calling "Madinina" as Columbus approached the island. The meaning was never discovered but was entered on early charts as Martinique, influenced by the name of St. Martin. (From Webster's New Geographical Dictionary, 1988, p734 & Room, Brewer's Dictionary of Names, 1992, p339)
The name of two islands of the West Indies, separated by a narrow channel. Their capital is Basse-Terre. They were discovered by Columbus in 1493, occupied by the French in 1635, held by the British at various times between 1759 and 1813, transferred to Sweden in 1813, and restored to France in 1816. Its status was changed from colony to a French overseas department in 1946. Columbus named it in honor of the monastery of Santa Maria de Guadalupe in Spain. (From Webster's New Geographical Dictionary, 1988, p470 & Room, Brewer's Dictionary of Names, 1992, p221)
A republic stretching from the Indian Ocean east to New Guinea, comprising six main islands: Java, Sumatra, Bali, Kalimantan (the Indonesian portion of the island of Borneo), Sulawesi (formerly known as the Celebes) and Irian Jaya (the western part of New Guinea). Its capital is Djakarta. The ethnic groups living there are largely Chinese, Arab, Eurasian, Indian, and Pakistani; 85% of the peoples are of the Islamic faith.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Tissue Plasminogen Activator
A proteolytic enzyme in the serine protease family found in many tissues which converts PLASMINOGEN to FIBRINOLYSIN. It has fibrin-binding activity and is immunologically different from UROKINASE-TYPE PLASMINOGEN ACTIVATOR. The primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases.
Amino Acid Sequence
Triple-looped protein domains linked by disulfide bonds. These common structural domains, so-named for their resemblance to Danish pastries known as kringlers, play a role in binding membranes, proteins, and phospholipids as well as in regulating proteolysis. Kringles are also present in coagulation-related and fibrinolytic proteins and other plasma proteinases.
Totipotent Stem Cells
Single cells that have the potential to form an entire organism. They have the capacity to specialize into extraembryonic membranes and tissues, the embryo, and all postembryonic tissues and organs. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Now, tissue engineers have the ability to remake many of the tissues in the body through the use of modern techniques such as ... Secondary cells A portion of cells from a primary culture is moved to a new repository/vessel to continue being cultured. ... Tissue engineered cultures also present additional problems in maintaining culture conditions. In standard cell culture, ... Tissue engineering often involves the use of cells placed on tissue scaffolds in the formation of new viable tissue for a ...
Plant tissue culture
... is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile ... like plant-derived secondary metabolites and recombinant proteins used as biopharmaceuticals. To cross distantly related ... The Plant Tissue Culture Information Exchange lists many commercial tissue culture labs. Since plant tissue culture is a very ... Necrosis can spoil cultured tissues. Generally, plant varieties differ in susceptibility to tissue culture necrosis. Thus, by ...
... is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell ... In contrast, immunohistochemical samples are sections of biological tissue, where each cell is surrounded by tissue ... In one such method, the antigen is bound by a primary antibody which is then amplified by use of a secondary antibody which ... There are many methods to obtain immunological detection on tissues, including those tied directly to primary antibodies or ...
Three American patients have received whole cultured bladders with the help of doctors who use nanobiology techniques in their ... The imaging of native biomolecules, biological membranes, and tissues is also a major topic for nanobiology researchers. Other ... Langer, Robert (2010). "Nanotechnology in Drug Delivery and Tissue Engineering: From Discovery to Applications". Nano Lett. 10 ... which causes secondary effects such as hair loss, fatigue or nausea killing not only cancerous cells but also the healthy ones ...
In vitro maturation
... to let a primordial follicle mature to a secondary follicle outside the body by growing it in a slice of ovarian tissue. The ... 8 days of culture in a serum-free medium 8 days of culture in a serum-free medium with activin A 4 days of culture on membranes ... This technique is also used in sheep, pigs and other animals. See In animals. Oocytes are classified depending on their ... Khalili MA, Dehghan M, Nazari S, Agha-Rahimi A (March 2014). "Assessment of ovarian tissues autografted to various body sites ...
Cancer stem cell
Many normal stem cells such as hematopoietic or stem cells from tissues, under special culture conditions, form three- ... Thus, the secondary more dominant CSCs may emerge, if a mutation confers more aggressive properties (Fig. 5). A study in 2014 ... labeling technique has been used to identify slow-cycling cells in animals) as genetic approaches are cell cycle independent ... SCS are embedded in tissue and persist in differentiated areas throughout tumor progression. MCS are located at the tumor-host ...
Central nervous system colonization is thought to be secondary to spread through blood and lymph tissue. The basis for the ... observation of pigmented fungal elements in affected tissues combined with the identification of the agent by culture or ... and selective techniques such as the use of high temperatures, mouse vectors, alkyl benzenes and mineral oils are required. ... Rhinocladiella mackenziei grows slowly in culture media. Colonies grown for a week at 30 °C on glucose peptone agar develop a ...
... can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyze the ... There are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect). Primary (direct) ... the use of the antibody-antigen relationship in tissues). This technique primarily makes use of fluorophores to visualise the ... but allows more flexibility because a variety of different secondary antibodies and detection techniques can be used for a ...
Electron microscopy is a common method that uses the immunolabeling technique to view tagged tissues or cells. The electron ... These secondary electrons are detected by a sensor, and the image of the specimen is generated over a certain time period. ... Moreover, cell cultures of human pituitary adenomas were viewed by light microscopy and immunocytochemistry, where these cells ... tissue, or organ. Antigens are organic molecules, usually proteins, capable of binding to an antibody. These antigens can be ...
Marburg virus disease
Primary infections occurred in Behringwerke laboratory staff while working with grivet tissues or tissue cultures without ... Secondary cases involved two physicians, a nurse, a post-mortem attendant, and the wife of a veterinarian. All secondary cases ... Medical staff should be trained in and apply strict barrier nursing techniques (disposable face mask, gloves, goggles, and a ... Hofmann, H.; Kunz, C. (1968). ""Marburg virus" (Vervet monkey disease agent) in tissue cultures". Zentralblatt für ...
Laboratory diagnosis of viral infections
To do this, antibodies that are specific to different types of viruses are mixed with the tissue sample. After the tissue is ... This technique makes many copies of the virus genome using virus-specific probes. Variations of PCR such as nested reverse ... Many viruses can be grown in cell culture in the lab. To do this, the virus sample is mixed with cells, a process called ... In the past nucleic acid tests have mainly been used as a secondary test to confirm positive serological results. However, as ...
If a serum or leak does not resolve (e.g., after a soft tissue biopsy), it may be necessary to take the patient back to the ... Larger seromas take longer to resolve than small ones, and they are more likely to undergo secondary infection. A seroma may ... it is recommended by some for the reason that seromas can be a culture medium for bacteria, whereas others advise it only for ... Tissues, and Disease : Principles of General Pathology: Principles of General Pathology. Oxford University Press. p. 435. ISBN ...
Sustainable and industrial production of guaianolides based on organ tissue culture, 2017-02-02, retrieved 2018-07-21. ... A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues". Journal of Visualized Experiments (136 ... Her work looks to identify the molecular and genetic regulation of the secondary metabolism in medicinal plants. She often ...
... but are becoming more common in cultivation with the advent of mass-production tissue-culture propagation techniques. Venus ... The vascular tissues of Sarracenia is a case in point. The keel along the front of the trap contains a mixture of leftward- and ... Carnivorous plant conservation will help maintain important ecosystems and prevent secondary extinctions of specialist species ... There also is a considerable list of Nepenthes endophytes; these are microbes other than pathogens that live in the tissues of ...
The first screening technique used is called ELISA. The hybridoma culture supernatant, secondary enzyme labeled conjugate, and ... other tissue aspirates, as well as lymph nodes and other tissues and can have increased sensitivity over normal ... The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which ... Multiwell plates are used initially to grow the hybridomas, and after selection, are changed to larger tissue culture flasks. ...
... using modern plant tissue culture methods. Micropropagation also referred as tissue culture is used to multiply plants such as ... the plant tissue grows and differentiates into new tissues depending on the medium. For example, media containing cytokinin are ... Experimental result also suggest that this technique can be successfully utilized for rapid multiplication of various plant ... often because the proper medium for growth is not known or the plants produce secondary metabolic chemicals that stunt or kill ...
Bacterial blight of cassava
In areas where bacterial wilt has not yet been established, it is important to raise a new crop from a meristem culture ... manihotis has been shown to survive asymptomatically for up to thirty months without new host tissue, but is a poor survivor in ... Several disease management techniques have been developed to control Cbb incidence and dissemination. Cultural practices have ... Because of its enzymes inability to break down highly lignified cell walls, this pathogen prefers to feed on younger tissues ...
Mass spectrometry imaging
... as animal and plant tissues and cell culture samples, without complex sample preparation Although, this technique has the ... In this version of the technique the sample, typically a thin tissue section, is moved in two dimensions while the mass ... Section 2.1, Secondary Ion Mass Specctrometry". In Banci, Lucia (ed.). Metallomics and the Cell. Metal Ions in Life Sciences. ... The two techniques used in MSI are: microprobe and microscope. This technique is performed using a focused ionization beam to ...
More detailed identification techniques involve the culture of infectious agents isolated from a patient. Culture allows ... allowed unusual access to deep tissues is a secondary infection. Primary pathogens often cause primary infection and also often ... In a microbial culture, a growth medium is provided for a specific agent. A sample taken from potentially diseased tissue or ... In the absence of suitable plate culture techniques, some microbes require culture within live animals. Bacteria such as ...
... culture techniques using Embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs). These are associated with ... number of clear advantages in using tissue-culture based protocols, some of which include reducing the cost of associated in ... Each layer gives rise to specific tissues and organs in the developing embryo. The ectoderm gives rise to epidermis, the ... Gilbert, Scott F. (2000). "Figure 10.20, [Organization of a secondary axis...]". www.ncbi.nlm.nih.gov. Retrieved 1 June 2020. ...
Frederick Campion Steward pioneered techniques of micropropagation and plant tissue culture controlled by plant hormones. The ... undergo a secondary growth phase resulting in two additional types of tissues: wood (secondary xylem) and bark (secondary ... and natural rubber are examples of commercially important materials made from plant tissues or their secondary products. ... Ethylene is a gaseous hormone that is produced in all higher plant tissues from methionine. It is now known to be the hormone ...
New staining techniques are being developed to differentiate bacterial cells growing in living animals, e.g. from tissues with ... from intraoperative cultures did not correspond to the bacteria species in the biofilm on the respective patient's tissue. In ... For example, many sewage treatment plants include a secondary treatment stage in which waste water passes over biofilms grown ... Removal techniques can also include antimicrobials. Dental plaque is an oral biofilm that adheres to the teeth and consists of ...
Veterinary public health
... animal tissue and other clinical waste. All of these materials have to be dealt with in a safe and controlled way. See the ... secondary processing and distribution followed by final preparation (all post-harvest). This "farm to fork" concept can be ... the transplantation of organs or tissues from one species to another) or the emergence of resistance to antimicrobial drugs due ... an animals who has had its genome deliberately altered by genetic engineering techniques as opposed to selective breeding) and ...
Stem cells can also be used to stimulate the growth of human tissues. In an adult, wounded tissue is most often replaced by ... It is a necessary requirement to develop a culture system to produce pure populations of tissue-specific stem-cell numbers in ... This controversy primarily targets the techniques used to derive new embryonic stem cell lines, which often requires the ... These cells involved in the secondary damage response secrete factors that promote scar formation and inhibit cellular ...
The septal mucosal tissue flap is the indicated technique for correcting defects of the distal half of the nose, and for ... where it is sutured to the deep tissues. Furthermore, a narrow wound, less than 1 mm wide can be allowed to heal by secondary ... and military punishment in that time and culture. ... a b c d e Principles and Techniques of Nasal Reconstruction at ... Surgical technique - the septal mucosal flap. The surgeon cuts the anteriorly based septal mucosal tissue-flap as widely as ...
Subcutaneous tissue. The subcutaneous tissue (also hypodermis and subcutis) is not part of the skin, but lies below the ... Society and culture. Hygiene and skin care. See also: Exfoliation (cosmetology) ... Development of techniques that increase skin permeability has led to more drugs that can be applied via transdermal patches and ... This protects the body from external particles such as toxins by not allowing them to come into contact with internal tissues. ...
Society and culture. Teeth whitening has become the most promoted and mentioned methodology in cosmetic dentistry. In ... With increasing age, teeth tend to be darker in shade. This can be attributed to secondary dentin formation and thinning of ... The organic matrix of dental plaque and calcified tissues undergo a series of chemical and morphological changes that lead to ... Hydrogen peroxide with concentrations of 10% or higher can cause tissue damage, be corrosive to mucous membranes and cause ...
Society and culture. Teeth whitening has become the most promoted and mentioned methodology in cosmetic dentistry. In ... With increasing age, teeth tend to be darker in shade. This can be attributed to secondary dentin formation and thinning of ... The organic matrix of dental plaque and calcified tissues undergo a series of chemical and morphological changes that lead to ... Greenwall, Linda (2017-04-11), "Tooth Sensitivity Associated with Tooth Whitening", Tooth Whitening Techniques, CRC Press, pp. ...
Stem cells can also be used to stimulate the growth of human tissues. In an adult, wounded tissue is most often replaced by ... The ability to grow up functional adult tissues indefinitely in culture through Directed differentiation creates new ... This controversy primarily targets the techniques used to derive new embryonic stem cell lines, which often requires the ... These cells involved in the secondary damage response secrete factors that promote scar formation and inhibit cellular ...
Cleft lip and cleft palate
However, cleft lip is more often portrayed negatively in popular culture. Examples include Oddjob, the secondary villain of the ... If these tissues fail to meet, a gap appears where the tissues should have joined (fused). This may happen in any single ... The bone tissue can be acquired from the patients own chin, rib or hip. A tympanostomy tube is often inserted into the eardrum ... Surgical techniques for cleft palate in dogs include prosthesis, mucosal flaps, and microvascular free flaps. Affected animals ...
... where the condition is confined to the mouth and perioral tissues, and secondary oral candidiasis, where there is involvement ... Oral swabs are taken if culture is required. Some recommend that swabs be taken from 3 different oral sites. Oral rinse ... Oral rinse technique can distinguish between commensal candidal carriage and candidiasis. If candidal leukoplakia is suspected ... Indeed, it can be clinically indistinguishable from true leukoplakia, but tissue biopsy shows candidal hyphae invading the ...
Comics As a Nexus of Cultures: Essays on the Interplay of Media, Disciplines. McFarland. p. 203. ISBN 978-0-7864-3987-4.. ... Once the shell is penetrated, the prey dies almost instantaneously, its muscles relax, and the soft tissues are easy for the ... Kassim, I.; Phee, L.; Ng, W. S.; Gong, F.; Dario, P.; Mosse, C. A. (2006). "Locomotion techniques for robotic colonoscopy". ... The skin consists of a thin outer epidermis with mucous cells and sensory cells, and a connective tissue dermis consisting ...
Fluorescence in situ hybridization
FISH is a very general technique. The differences between the various FISH techniques are usually due to variations in the ... First, an infected tissue sample is taken from the patient. Then an oligonucleotide complementary to the suspected pathogen's ... Some assays are designed so that the secondary color will be present or absent in cases of interest. An example is the ... If a patient is infected with a suspected pathogen, bacteria, from the patient's tissues or fluids, are typically grown on agar ...
Chronic obstructive pulmonary disease
Society and culture. See also: COPD Awareness Month. COPD is known colloquially as "smoker's lung", but it may also occur ... For the abnormal occurrence of gas within tissue, see pneumatosis. For the long-term productive cough, see Bronchitis § Chronic ... Osadnik CR, McDonald CF, Jones AP, Holland AE (March 2012). "Airway clearance techniques for chronic obstructive pulmonary ... which may cause right-sided heart failure secondary to lung disease, also known as cor pulmonale. ...
Society and cultureEdit. Comparisons with medical cannabisEdit. Further information: Medical cannabis ... Like most pharmacologically active secondary metabolites of plants, THC is a lipid found in cannabis, assumed to be involved ... THC is highly lipophilic and initially taken up by tissues that are highly perfused, such as the lung, heart, brain, and liver. ... oral fluid or sweat using a combination of immunoassay and chromatographic techniques as part of a drug use testing program or ...
... present in mature tissue. Virchow believed that cancer is caused by severe irritation in the tissues, and his theory came ... kultur, cultur, culture + kampf fight.] (Ger. Hist.) Lit., culture war; - a name, originating with Virchow (1821-1902), given ... Prosector was a secondary position in the hospital. This secondary position in Berlin convinced him to accept the chair of ... The modern autopsy still constitutes his techniques. His first significant autopsy was on a 50-year-old woman in 1845. He ...
Major complications are spreading of the abscess material to adjacent or remote tissues, and extensive regional tissue death ( ... Ladde JG, Baker S, Rodgers CN, Papa L (2015). "The LOOP technique: a novel incision and drainage technique in the treatment of ... Society and culture. The Latin medical aphorism "ubi pus, ibi evacua" expresses "where there is pus, there evacuate it" ... An incisional abscess is one that develops as a complication secondary to a surgical incision. It presents as redness and ...
Since early in the 1980s, fetal, porcine, carotid or retinal tissues have been used in cell transplants, in which dissociated ... Other effective techniques to promote relaxation include slow rotational movements of the extremities and trunk, rhythmic ... CT and MRI are also used to rule out other diseases that can be secondary causes of parkinsonism, most commonly encephalitis ... An additional significant problem was the excess release of dopamine by the transplanted tissue, leading to dystonias. ...
Secondary neoplasm. Development of secondary neoplasia after successful chemotherapy or radiotherapy treatment can occur ... This allows a high dose to cancer tissues with a relatively low dose to other tissues. As different proteins are utilised by ... McKnight JA (May 2003). "Principles of chemotherapy". Clinical Techniques in Small Animal Practice. 18 (2): 67-72. PMID ... Makin G, Hickman JA (Jul 2000). "Apoptosis and cancer chemotherapy". Cell and Tissue Research. 301 (1): 143-52. Bibcode: ...
Treatment duration is typically 7-10 days with the type of antibiotic used directed by the results of cultures. If the culture ... The goal is to optimize oxygen delivery to tissues and achieve a balance between systemic oxygen delivery and demand. An ... During critical illness, a state of adrenal insufficiency and tissue resistance to corticosteroids may occur. This has been ... However, sodium bicarbonate is not recommended for a person with lactic acidosis secondary to hypoperfusion. Low-molecular- ...
pH-dependent primary, secondary, or tertiary amines; primary and secondary amines become positively charged at pH , 10: ... Detergents are key reagents to extract protein by lysis of the cells and tissues: They disorganize the membrane's lipid bilayer ... Finning techniques. *Scuba skills *Buddy breathing. *Low impact diving *Diamond Reef System ... Safety culture *Code of practice. *Diving safety officer. *Diving superintendent. *Health and safety representative ...
Prospects as a therapeutic techniqueEdit. This section needs to be updated. Please update this article to reflect recent events ... The RNA interference pathway is often exploited in experimental biology to study the function of genes in cell culture and in ... In C. elegans this initiation response is amplified through the synthesis of a population of 'secondary' siRNAs during which ... To achieve the clinical potential of RNAi, siRNA must be efficiently transportated to the cells of target tissues. However, ...
It works by damaging the DNA of cancerous tissue, killing it. To spare normal tissues (such as skin or organs, which radiation ... In other cultures, other rights and values are preferred. For example, most African cultures value whole families rather than ... Most complementary and alternative medicines for cancer have not been studied or tested using conventional techniques such as ... "156: Secondary Cancers: Incidence, Risk Factors, and Management". In Frei E, Kufe DW, Holland JF. Holland-Frei Cancer Medicine ...
Scanziani E (1998). "Immunohistochemical staining of fixed tissues". Methods Mol Biol. 104: 133-140. doi:10.1385/0-89603-525-5: ... The fused cells are called hybridomas, and will continually grow and secrete antibody in culture. Single hybridoma cells are ... Rosetta Antibody is a novel antibody FV region structure prediction server, which incorporates sophisticated techniques to ... Milland J, Sandrin MS (December 2006). "ABO blood group and related antigens, natural antibodies and transplantation". Tissue ...
Hausa culture has it that a child with a fallen tooth should not let a lizard see the toothless gum because if a lizard does ... This technique has limited use when pulp is exposed due to injury but is generally not accepted for managing carious pulp ... In cases of extensive tooth decay, the pulp must be treated to maintain the health of the tooth and its supporting tissues. In ... often because its secondary tooth failed to develop. Primary teeth are essential in the development of the mouth. The primary ...
Native platinum used by ancient Ethiopians and by South American cultures always contained a small amount of the other platinum ... A coating of iridium also increases the signal to noise ratio associated with secondary electron emission which is essential to ... there are only about 20 parts per trillion of iridium in human tissue. Like most metals, finely divided iridium powder can be ... Iridium in bulk metallic form is not biologically important or hazardous to health due to its lack of reactivity with tissues; ...
As well as furthering research on animal implantable devices, experiments on cultured neural tissue have focused on building ... In June 2013 it was announced that Bin He had developed the technique to enable a remote-control helicopter to be guided ... After the surgery, scar tissues may form which can make brain signals weaker. In addition, according to the research of ... In a secondary, implicit control loop the computer system adapts to its user improving its usability in general. Beyond BCI ...
History of medicine
Tissue culture is important for development of vaccines. Though the early success of antiviral vaccines and antibacterial drugs ... hospitals could have a mix of culture and techniques. This allowed for a sense of collaborative medicine. Hospitals became ... If they had places of worship, they were secondary to the medical side of the hospital. Islamicate hospitals, along with ... used to create tiny robotic devices to assist microsurgery using micro-video and fiber-optic cameras to view internal tissues ...
History of the Tesla coil
... secondary winding. One of Tesla's early coils at his New York lab in 1892, with a conical secondary. Prototype "magnifying ... In order to apply the electrode directly to the skin, or tissues inside the mouth, anus or vagina, a "vacuum electrode" was ... A part of the field that survived was diathermy, the application of high frequency current to heat body tissue, pioneered by ... Skal, David J. (1998). Screams of Reason: Mad Science and Modern Culture. W. W. Norton and Co. pp. 89-90. ISBN 978-0393045826. ...
Zheng H, Fu R, Wang JT, Liu Q, Chen H, Jiang SW (April 2013). "Advances in the techniques for the prediction of microRNA ... Recent work on miR-430 in zebrafish, as well as on bantam-miRNA and miR-9 in Drosophila cultured cells, shows that ... miRNA research revealed different sets of miRNAs expressed in different cell types and tissues and multiple roles for miRNAs in ... MiR-712 targets tissue inhibitor of metalloproteinases 3 (TIMP3). TIMPs normally regulate activity of matrix metalloproteinases ...
If absent or deficient of natural tissue, alternatives can be cultured sheets of epithelial cells in vitro or synthetic ... Microsurgery is generally concerned with the reconstruction of missing tissues by transferring a piece of tissue to the ... Reconstructive surgery techniques were being carried out in India by 800 BC. Sushruta was a physician who made important ... Adult craniofacial surgery deals mostly with fractures and secondary surgeries (such as orbital reconstruction) along with ...
Nanomaterials can be toxic to human tissue and cell cultures (resulting in increased oxidative stress, inflammatory cytokine ... Then, once in the blood stream, nanomaterials can be transported around the body and be taken up by organs and tissues, ... through a secondary genotoxicity mechanism that is not specific to TiO2 but primarily related to particle size and surface area ... and biological samples since current detection techniques require expensive and complex analytical instrumentation. Toxicology ...
Outline of the human brain
Culture in music cognition the impact that a person's culture has on their music cognition, including their preferences, ... Neuropathology - biopsy of tissue from the brain and spinal cord to aid in diagnosis of disease. Frequent neurosurgery ... Devin Galligan underwent a special form of brain mapping surgery paired with sophisticated anesthesiology techniques whereby ... deficiency usually secondary to hazardous alcohol use causing vision changes, ataxia, and impaired memory. Alcoholic ...
An alternative to germinated seed, once constraints to mass production are overcome, are tissue-cultured or "clonal" palms, ... This is often followed by secondary pathogenic infections in the spear that can lead to spear rot and palm death. Cadang-cadang ... doi:10.1007/s11557-015-1123-2. Idris, A S; Kushairi, D; Ariffin, D; Basri, M W (June 2006). "Technique for inoculation of oil ... bacteria are organisms inhabiting plant organs that at some time in their life cycles can colonize the internal plant tissues ...
The neural crest is the germ-layer derived tissue that welds the visceral and somatic divisions together. The developmental ... ISBN 978-0-738-20680-6. Hall, Brian K. (1998), "Germ Layers and the Germ-Layer Theory Revisited: Primary and Secondary Germ ... advances in computational techniques and the power to model population dynamics. Edelman, and his team, were positioned in time ... Culture is ancestor knowledge transmission. Adaptive pattern recognition in humans is as much social as it is individual. ...
This resulted in damage to both bacterial cells and human tissue. Other sanitizing techniques, such as irrigation with ... This same high oxidizing potential, however, causes ozone to damage mucous and respiratory tissues in animals, and also tissues ... The United States Environmental Protection Agency is proposing a secondary regulation to reduce crop damage, in addition to the ... Noble, A.C.; Summerfelt, S.T. (1996). "Diseases encountered in rainbow trout cultured in recirculating systems". Annual Review ...
The retrospect and prospect of the applications of biotechnology in Phoenix dactylifera L. | SpringerLink
El-Sharabasy SF (2000) Studies on the production of secondary metabolites from date palm by using tissue culture technique. Ph. ... El-Dawayati MM (2008) Using tissue culture technology to storage some plant tissues of date palm. Ph.D. Thesis, Department of ... tissue and organ culture, bioreactor-mediated large-scale propagation, cell suspension culture, embryogenic culture, protoplast ... Effect of amino acids on the growth and production of steroids in date palm using tissue culture technique. Researcher 4:75-84 ...
PPT - Plant Tissue Culture PowerPoint Presentation - ID:346170
the culture of plant seeds, organs, explants, tissues, cells, or protoplasts on nutrient media under sterile conditions. ... What Is plant tissue culture ?. Or in vitro culture? Or in vitro propagation? Or Micropropagation ?. Definition. ... Plant Tissue Culture -. t.c. refers to technique of growing plant cells, tissues, organs, seeds or other plant parts in a ... Secondary metabolites produced by plants. *Alkaloids, Terpenoids, Steroids, Anthocyanins, Anthraquinones, Polyphenols. *Often ...
Phytochemical and Pharmacological Properties of Gymnema sylvestre: An Important Medicinal Plant
Cultured plant cells and tissues are widely recognized as promising alternatives for the production of valuable secondary ... Various techniques were employed for propagation of the herb in plant tissue culture through in vitro multiplication for shoot ... in plant tissue culture offers a promising alternative for the production of valuable secondary metabolite. G. sylvestre, being ... Plant tissue culture techniques have been employed on an industrial scale for the production of bioactive compounds . ...
Professor Mick Fuller - University of Plymouth
and Salviaofficinalis L. using plant tissue culture techniques (in partnership with Suliamaini University, Suliamania, ... Rihan H & Fuller M 2018 An Effective Protocol for the Production of Primary and Secondary Somatic Embryos in Cauliflower ( ... Rihan HZ, Al-Shamari M & Fuller MP 2012 The Production of Cauliflower Microshoots using Curd Meristematic Tissues and ... 2013 Evaluation and selection of barley genotypes for salt stress resistance using tissue culture techniques and SDS-PAGE gel ...
application of plant tissue culture in horticulture biology discussion
Plant tissue culture is an important technique for the production of secondary metabolites in large quantities. (i) Shooting ... and from cultured tissues. Research on protoplast culture in carrot, the model species for plant tissue culture systems and the ... I. Tissue Culture: One of the widest applications of biotechnology has been in the area of tissue culture and micro propagation ... These cultures are carried out in specially designed culture vessels (bioreactors). Application of plant tissue culture in ...
Rollinia mucosa (Jacq.) Baill.: establishment of callus culture and lignan production
The potential of tissue culture techniques for the production of several secondary metabolites has been known for many years. ... It is evident that callus tissues of R. mucosa are an alternative source of EPIE, EPIY and MAG being a attractive technique for ... Hagen SR, Muneta P, Augustin J, Letourneau D. Stability and utilization of picloram, vitamins, and sucrose in a tissue culture ... These results confirm that the production of secondary metabolites in callus culture is not dependent only of the callus growth ...
Therapeutic use of medicinal plants and their extracts: Volume 1 | Weltbild.ch
v. Animal tissue culture technique vi. Animal products vii. Fermentation and production of microbial primary and secondary ... The plant tissues and tissue systems- meristematic and permanent tissue, simple and complex tissue iii. Secretory tissues and ... Plant tissue and organ culture techniques iii. Production of drug principles by tissue, organ culture and production ... Development and application of tissue cultures technique for naturally rare and slow growing high value plant species for cost- ...
Plant biotechnology - Gate2Biotech.com
... and protoplasts and the technique of protoplast fusion. Tissue culture is the technique of maintaining plant tissues ... Tissue culture is very useful for: • in vitro production of secondary metabolites, • in vitro propagation (cryopreservation, ... Although plant tissue culture is most often carried out on solid medium, containing agar, tissues can also be cultured in ... cell suspension cultures, hairy root cultures, stirred tank bioreactors), • cell, tissue and embryomanipulation (embryo rescue ...
Patent US6630327 - Methods and reagents for preparing and using immunological agents specific ... - Google Patents
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NOVEL PLANT TISSUE CULTURE SYSTEMS EMPLOYED AS ALTERNATIVE TECHNOLOGIES TO PRODUCE SECONDARY METABOLITES-(Abstract Only) ... TECHNIQUES TO IMPROVE GROWTH, MORPHOGENESIS, AND SECONDARY METABOLISM RESPONSES FROM LAMIACEAE SPECIES IN VITRO-(Peer Reviewed ... Modern Phytochemical Analysis: Evaluation in Plant Tissues and Processed Products-(Abstract Only) Berhow, M.A., Duval, S.M., ... ESTABLISHING TISSUE-CULTURED SWEETGUM PLANTS IN SOIL-(Peer Reviewed Journal) Tisserat, B. 2005. Establishing tissue-cultured ...
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- Abahmane L (2013) Recent achievements in date palm ( Phoenix dactylifera L.) micropropagation from inflorescence tissues. (springer.com)
- Our well-known cauliflower cloning practical is based on a micropropagation technique developed by Jonathan's colleagues at Kew. (saps.org.uk)
- New topic discussions and laboratory exercises in the Second Edition include "Micropropagation of Dieffenbachia," "Micropropagation and in vitro flowering of rose," "Propagation from nonmeristematic tissue-organogenesis," "Variation in culture" and "Tissue culture of ferns. (routledge.com)
- Clonal Propagation (Micropropagation) : One of the most important aspects of plant tissue culture technology is its use in clonal propagation of selected species of crops and ornamentals. (preservearticles.com)
- Plant cell culture is considered as a useful technique for the large scale production of plant secondary metabolites under in vitro level beside its utility for micropropagation. (springeropen.com)
- The tissue culture methods of sow procreation, renowned as 'micropropagation' utilizes the culture of apical shoots, axillary buds and meristems on apposite nutrient standard.The renaissance of plantlets in educated tissue was described by Murashige in 1974. (ebioworld.com)
- The micropropagation is rapid and has been adopted pro commercialization of valuable plants such as banana, apple, pears, strawberry, cardamom, many ornamentals (e.G. Orchids) and other plants.The micropropagation techniques are preferred ended the square asexual procreation methods since of the following reasons: (a) inside the micropropagation method, single a small amount of tissue is vital to rekindle millions of clonal plants in a time. (ebioworld.com)
- The steps in micropropagation method are: A) Initiation of culture - from an explant like spurt tip on a apposite nutrient standard, b) multiple shoots formation from the educated explant, c) rooting of in vitro urban shoots and, d) transplantation - transplantation to the meadow following acclimatization. (ebioworld.com)
- Abd El Bar OH, El-Dawayati MM (2014) Histological changes on regeneration in vitro culture of date palm (' Phoenix dactylifera ') leaf explants. (springer.com)
- 5 Lignan production in R. mucosa tissues was also observed both in in vivo grown seedlings and in in vitro propagated plants derived from hypocotyl explants. (sld.cu)
- Callus cultures were initiated from organs of two-month-old nursery-grown seedlings and from in vitro propagated plants derived from hypocotyl explants of R. mucosa , cultivated on medium with 2,3 m M KIN + 2,2 m M BA7. (sld.cu)
- Tissue culture is a technique whereby small pieces of viable tissue (called 'explants') are isolated from parent plants and grown in a defined nutritional and controlled environment for prolonged period under aseptic conditions. (preservearticles.com)
- For successful plant tissue culture it is best to proceed with an explants rich in undermined cells, e.g. those from the cortex or meristem, because such cells are capable of rapid proliferation. (preservearticles.com)
- The explants to proceed with tissue culture technique in plants are usually taken from leaves, buds, root apex, shoot-spex, nodal segments or germinating seeds. (preservearticles.com)
- These hormones vary for different tissue explants from different parts of the same plant and for the same explants from different genera of plants. (preservearticles.com)
- TcLEC2 overexpressing cotyledon explants exhibited a very high level of embryogenic competency and when cultured on hormone free medium, exhibited an iterative embryogenic chain-reaction. (biomedcentral.com)
- it is usually on solidified medium and initiated by inoculation of small explants or sections from established organ or other cultures (the inocula). (fao.org)
- Zana Kareem PhD (2013-2016) Production of some secondary metabolites from Datura innoxia Mill. (plymouth.ac.uk)
- Plant tissue culture is an important technique for the production of secondary metabolites in large quantities. (reviewsic.com)
- The potential of tissue culture techniques for the production of several secondary metabolites has been known for many years. (sld.cu)
- The qualitative and quantitative improvement of biologically active compounds by plant cell, tissue and organ culture have been used as an alternative source for the production of important bioactive secondary metabolites using different strategies of metabolic engineering. (springeropen.com)
- The biotechnological production of valuable secondary metabolites in plant cell or organ cultures is a good alternative to the extraction of whole plant material because many plants of high medicinal products are difficult to cultivate. (springeropen.com)
- The attractive concepts in the scaling up technology of natural products are enhancement of secondary metabolites and use of biological and chemical elicitors. (springeropen.com)
- In addition, in vitro somatic embryogenesis (SE) tissue culture offers an approach to speed up the development and deployment of genetically improved genotypes because of its potentially very high multiplication rate and scalability. (biomedcentral.com)
- Plant protoplasts (i.e., cells devoid of cell walls) are also used in the laboratory for culture. (reviewsic.com)
- Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition , addresses the most current principles and methods in plant tissue culture research. (routledge.com)
- In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition , is a veritable harvest of information for the continued study and research in plant tissue culture science. (routledge.com)
- The appeal of this practical manual is that it contains very detailed information on how to establish a tissue culture laboratory, from provision of lists of equipment and chemicals and their suppliers, to procedures and protocols for preparing media and carrying out experiments…I would recommend this book highly for teachers and researchers in plant tissue culture as a valuable resource for practical plant tissue culture. (routledge.com)
- Patterns of gene expression are reduced in complexity in cell culture models, suggesting that heterogeneity is lost once a tumor is removed from a patient and cultured in the laboratory ( 1 ). (aacrjournals.org)
- In 1910, Carrel's assistant, Montrose Burrows, visited Harrison's laboratory at Yale University for several months to learn about tissue culture. (asu.edu)
- Train laboratory staff on different experimental techniques used in the core facility. (academiccareers.com)
- Scientific advances in biomaterials, stem cells, growth and differentiation factors, and biomimetic environments have created unique opportunities to fabricate or improve existing tissues in the laboratory from combinations of engineered extracellular matrices ("scaffolds"), cells, and biologically active molecules. (wikipedia.org)
- Among the major challenges now facing tissue engineering is the need for more complex functionality, biomechanical stability, and vascularization in laboratory-grown tissues destined for transplantation. (wikipedia.org)
- Many different recipes for tissue culture media are available and each laboratory must determine which medium best suits their needs. (docplayer.net)
- Following the accidental infection of a laboratory worker with San Miguel sea lion virus, a related virus was isolated in cell culture and named Hom-1. (asm.org)
- To provide knowledge about animal cell culture which has become an important and essential laboratory technique for the study of biochemical, physiological and pharmacological processes. (atilim.edu.tr)
- This article compares the different biotechnological techniques used in this species such as: tissue and organ culture, bioreactor-mediated large-scale propagation, cell suspension culture, embryogenic culture, protoplast culture, conservation (for short- and long-term) of germplasms, in vitro mutagenesis, in vitro selection against biotic and abiotic stresses, secondary metabolite production in vitro, and genetic transformation. (springer.com)
- besides, it would undeniably facilitate the tissue culture-based research on date palm for accelerated propagation and enhanced production of quality planting materials, along with conservation and exchange of germplasms, and genetic engineering. (springer.com)
- I. Tissue Culture: One of the widest applications of biotechnology has been in the area of tissue culture and micro propagation in particular. (reviewsic.com)
- Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. (routledge.com)
- Several methods of vegetative propagation are commonly used with cocoa (grafting and rooted cuttings techniques). (biomedcentral.com)
- The most important factor in tissue culture technology is the maintenance of aseptic conditions. (preservearticles.com)
- Maintenance of cells in long-term culture requires strict adherence to aseptic technique to avoid contamination and potential loss of valuable cell lines (see UNIT 1.3). (docplayer.net)
- NOTE: All solutions and equipment coming into contact with living cells must be sterile, and aseptic technique should be used accordingly. (docplayer.net)
- Aseptic technique, gentle tissue handling, and hemostasis are essential. (vin.com)
- The two biologists also expanded the application of tissue culture to tissues of various types, including adult tissues, tissues of mammalian origin, and cancerous tissues from chicks and humans . (asu.edu)
- 1 Basic Techniques for Mammalian Cell Tissue Culture UNIT 1.1 Tissue culture technology has found wide application in the field of cell biology. (docplayer.net)
- Callus cultures were established from different organs of seedlings and in vitro propagated plants of Rollinia mucosa . (sld.cu)
- Callus cultures were initiated from hypocotyl, epicotyl, young leaves (2º and 3º nodes) and mature leaves (4º and 5º nodes) from seedlings. (sld.cu)
- Establishment of callus cultures. (sld.cu)
- Callus cultures can be indefinitely maintained through regular sub-culturing. (fao.org)
- At a steady state, the rate of cells removed from the cultures equals to the rate of formation of new cells. (reviewsic.com)
- Measurement of Viability of Cultured Cells: Culture of Isolated Single Cells (Single Cell Clones): The best answers are voted up and rise to the top. (reviewsic.com)
- Tissue culture produces clones in which all product cells have the same genotype. (reviewsic.com)
- Plant leaves with homogenous population of cells are the ideal sources for cell culture. (reviewsic.com)
- As the cells are incubated in suspension cultures, the cells divide and enlarge. (reviewsic.com)
- The term 'plant tissue culture' is normally used as a 'blanket' term to cover the cultivation of all plant parts whether a single cell, a group of cells or an organ on an artificial medium. (reviewsic.com)
- Viability of cultured cells can be measured by microscopic examination of cells directly or after staining them. (reviewsic.com)
- Dedifferentiation is possible since the non- dividing quiescent cells of the explant, when grown in a suitable culture medium revert to meristematic state. (reviewsic.com)
- They are the culture of tissue, cells, and protoplasts and the technique of protoplast fusion. (gate2biotech.com)
- As with tissue culture, cells regenerated from protoplasts can be induced to divide and form callus and, eventually, to regenerate fertile plants. (gate2biotech.com)
- Plant cell/tissue culture is the in-vitro culture of sterilised plant cells, tissues and/or organs on a nutrient medium. (saps.org.uk)
- The receptor affinity probe or receptor alkaline phosphatase (RAP) staining method uses soluble protein ectodomains fused to secreted placental alkaline phosphatase to locate ectodomain binding sites within cells or tissues. (sciencemag.org)
- We have used this approach to identify expressing cells in tissue culture, in tissue sections, or in whole-mount embryos. (sciencemag.org)
- The selective pressure of cell culture allows the least differentiated cells to thrive, resulting in distinct and irreversible losses of important biologic properties ( 1 ). (aacrjournals.org)
- Presented in Figure 2 is a comparison of living cells in culture imaged in both brightfield and phase contrast illumination. (microscopyu.com)
- The cells are human glial brain tissue grown in monolayer culture bathed with a nutrient medium containing amino acids, vitamins, mineral salts, and fetal calf serum. (microscopyu.com)
- The publicized work of Carrel and his associates at the Rockefeller Institute established the practice of long-term tissue culture for a wide variety of cells. (asu.edu)
- Burrows successfully established tissue cultures of embryonic chick cells. (asu.edu)
- Experimentally, Carrel and Burrows adapted Harrison's hanging drop method of amphibian embryonic cells for wider applications and so that tissues could be cultured for a longer duration. (asu.edu)
- Although the serial culturing technique could extend the life span of the cultures, Carrel noticed that the size of the cells decreased with each subculture. (asu.edu)
- The "embryo juice" was made by grinding up embryonic tissues, mixing them with saline solution, and eliminating remaining cells through centrifugation and filtration. (asu.edu)
- He eventually managed to keep the steady growth of embryonic chick heart tissues through serial cultivation for thirty-four years, leading him to conclude that cells were intrinsically immortal. (asu.edu)
- Among many speculations proposed to account for how Carrel's chick heart tissue could have survived in tissue culture for so long, was the question of whether the "embryo juice" was in fact introducing extra living cells that had not been eliminated by the centrifugation and filtration processes. (asu.edu)
- Design, and carry out experiments involving collection and processing of tissues and tissue culture cells and their analysis by immunofluorescence, live-cell imaging, confocal, two-photon, lightsheet, TIRF and super-resolution microscopy. (academiccareers.com)
- Tissue engineering is a biomedical engineering discipline that uses a combination of cells, engineering, materials methods, and suitable biochemical and physicochemical factors to restore, maintain, improve, or replace different types of biological tissues. (wikipedia.org)
- Tissue engineering often involves the use of cells placed on tissue scaffolds in the formation of new viable tissue for a medical purpose but is not limited to applications involving cells and tissue scaffolds. (wikipedia.org)
- The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues. (wikipedia.org)
- He proposed the joining of the terms tissue (in reference to the fundamental relationship between cells and organs) and engineering (in reference to the field of modification of said tissues). (wikipedia.org)
- Materials that may harbor infectious organisms such as human or primate tissues, fluids, cells, cell cultures. (powershow.com)
- Extensive control experiments, including clonal analysis, demonstrated that failure of neurosphere growth was not merely secondary to loss of GFAP-expressing support cells or the result of a nonspecific toxic effect. (jneurosci.org)
- Cell-sorting techniques have been used to examine molecular characteristics of putative adult NSCs identified as neurosphere-forming cells. (jneurosci.org)
- In most cases, cells or tissues must be grown in culture for days or weeks to obtain sufficient numbers of cells for analysis. (docplayer.net)
- An important factor influencing the growth of cells in culture is the choice of tissue culture medium. (docplayer.net)
- The first step in subculturing monolayers is to detach cells from the surface of the primary culture vessel by trypsinization or mechanical means. (docplayer.net)
- TRYPSINIZING AND SUBCULTURING CELLS FROM A MONOLAYER A primary culture is grown to confluency in a 60-mm petri plate or 25-cm 2 tissue culture flask containing 5 ml tissue culture medium. (docplayer.net)
- Cells are dispersed by trypsin treatment and then reseeded into secondary cultures. (docplayer.net)
- The process of removing cells from the primary culture and transferring them to secondary cultures constitutes a passage, or subculture. (docplayer.net)
- The medium contains unattached cells that may attach and grow, thereby providing a backup culture. (docplayer.net)
- Check culture with an inverted microscope to be sure that cells are rounded up and detached from the surface. (docplayer.net)
- Similarly, if the tissue from which the variants be inflicted with been obtained is having gametophytic origin such as pollen or egg cell, it is renowned as 'gametoclonal' alteration.They explained with the intention of it could be due to: (a) reflection of heterogeneity linking the cells and explant tissue, (b) a unadorned representation of spontaneous change rate, and (c) initiation by culture background of transposition of genetic equipment. (ebioworld.com)
- In this study, we found that Hom-1 could be propagated in a number of human cell lines, making it the first calicivirus to replicate efficiently in cultured human cells. (asm.org)
- A protective tissue, consisting of parenchyma cells, that develops over a cut or damaged plant surface. (fao.org)
- 3. Actively dividing non-organized masses of undifferentiated and differentiated cells often developing from injury (wounding) or in tissue culture in the presence of growth regulators. (fao.org)
- A layer, usually regarded as one or two cells thick, of persistently meristematic tissue between the xylem and phloem tissues, and which gives rise to secondary tissues, thus resulting in an increase in diameter. (fao.org)
- cancer Uncontrolled growth of the cells of a tissue or an organ in a multicellular organism. (fao.org)
- Microfabricated systems provide an excellent platform for the culture of cells, and are an extremely useful tool for the investigation of cellular responses to various stimuli. (mdpi.com)
- For instance, researchers at the University of Washington are studying single cells using MEMS tools because conventional cell culture systems provide only an average from a population of cells [ 14 ]. (mdpi.com)
- Plants in culture usually cannot meet their needs for fixed carbon. (slideserve.com)
- This work had as first objective the establishment of a protocol for callus culture of R. mucosa by manipulating different types and combinations of plant growth regulators and as second objective the increasing and/or inducing of furofuranic lignans production in calli derived from organs of seedlings and in in vitro propagated plants. (sld.cu)
- Although modern biotechnology is based on advances in molecular genetics and recombinant DNA technology, exploitation of these advances in plants has been facilitated by two other established techniques in plant physiology. (gate2biotech.com)
- With a relatively small investment in space, technical support, and materials, tissue culture has made it possible to produce literally millions of high-quality, genetically uniform plants. (gate2biotech.com)
- In this resource, students carry out a 'cauliflower cloning' tissue culture practical to investigate totipotency in plants. (saps.org.uk)
- Most of the research is directed towards the development of improved plants for agriculture, horticulture and forestry using tissue culture methods. (brainkart.com)
- Several laboratories are being supported by providing funds for development of tissue culture technology for the improvement of crop plants. (brainkart.com)
- It has been experimental with the intention of the long-term hard skin and cell suspension culture and plants regenerated from such cultures are often associated with chromosomal variations. (ebioworld.com)
- carcinoma A malignant tumour derived from epithelial tissue, which forms the skin and the outer cell layers of internal organs. (fao.org)
- Tissue culture is the technique of maintaining plant tissues indefinitely on an artificial medium. (gate2biotech.com)
- Necrotizing cholecystitis more commonly affects middle-aged to older adult dogs and may develop secondary to thromboembolism, blunt abdominal trauma, bacterial infection, EHBDO (cystic duct obstruction or distal duct obstruction by choleliths, stricture, or neoplasia), or a mature gallbladder mucocele (causing tense gallbladder distention). (merckvetmanual.com)
- Prior to the invention of phase contrast techniques, transmitted brightfield illumination was one of the most commonly utilized observation modes in optical microscopy, especially for fixed, stained specimens or other types of samples having high natural absorption of visible light. (microscopyu.com)
- In 1907 Harrison successfully carried out the first tissue culture using the hanging drop method , in which fragments of embryonic frog nerve tissue were grown in a clot of lymphatic fluid. (asu.edu)
- Applications: Plant tissue culture is an essential component of plant biotechnology. (reviewsic.com)
- Protoplasts can be isolated from virtually any plant tissue and, in many cases, remain capable of sustained cell division. (gate2biotech.com)
- This serial cultivation was also the basis of Carrel's 1912 paper "On the Permanent Life of Tissues outside of the Organism. (asu.edu)
- Secondary bacteremia occurs secondary to infection in other sites (e.g. skin and soft tissues), pneumonia , and infection in wounds. (medindia.net)
- Avulsion wounds are characterized by tearing of tissues from their attachments and creation of skin flaps. (vin.com)
- Crush injuries can be a combination of other types of wounds with extensive damage and contusions to skin and deeper tissue. (vin.com)
- however, some wounds heal by contraction and epithelialization (healing by secondary intention). (vin.com)
- Severely contaminated or infected wounds should be cultured after initial inspection. (vin.com)
- Differentiation: While studying totipotency, it is stated that the dedifferentiation and redifferentiation … Cell cultures can also be used as factories for bioconversion of intermediate compounds into more valuable products. (reviewsic.com)
- The sub-cultured callus is now induced to proceed for further development and, finally, differentiation. (preservearticles.com)
- In embryo culture, the plant develops directly from the embryo or indirectly through the formation of callus and then subsequent formation of shoots and roots. (reviewsic.com)
- Although plant tissue culture is most often carried out on solid medium, containing agar, tissues can also be cultured in liquid medium (for example somatic embryo culture). (gate2biotech.com)
Development of tissue culture1
- Thus Carrel's legacy had a mixed influence on the development of tissue culture techniques, which have been widely used in the fields of embryology and stem cell research. (asu.edu)
- The purpose of this study is to offer an alternative method to women who wish to preserve the possibility of fertility, as well as to learn more about the ability of human eggs to survive and function after long term storage in frozen ovaries (ovarian tissue cryopreservation). (clinicaltrials.gov)
- We will also retrospectively enroll women who have already undergone this procedure by contacting them by mail and offering participation in our ovarian tissue cryopreservation database. (clinicaltrials.gov)
- Determine the efficacy of ovarian cryopreservation techniques. (clinicaltrials.gov)
- The most significant innovation described in their early publications was probably the method designed for serial cultivation of tissues. (asu.edu)
- In serial cultivation fragments of one culture are transferred to new media to form secondary cultures, which ensures that the growth of tissues can be maintained for longer periods. (asu.edu)
- Carrel and Burrows called this prolonged culturing "reactivation and cultivation in series. (asu.edu)
- In 1913 he found that cell size could be maintained by adding extracts of homogenized tissues from chicks, especially of embryonic origin. (asu.edu)
- This supplemental extract from embryonic tissues, which he called "embryo juice," proved to be potent in "activating" growth in tissue cultures. (asu.edu)
- Soluble ectodomain placental alkaline phosphatase fusion proteins are therefore highly sensitive reagents that permit the direct localization of available binding sites through simple chromogenic assays without purification, radioactive labeling, or secondary reagents. (sciencemag.org)
- Cloning cauliflowers" is a popular way to look at tissue culture and totipotency. (saps.org.uk)
- Incumbent will also assist with advanced tissue preparation methods (e.g. tissue clearing, expansion microscopy), image processing (e.g. deconvolution), and data analysis methods (e.g. (academiccareers.com)
- Perform advanced sample preparation for high resolution light microscopy techniques, such as tissue clearing and expansion microscopy. (academiccareers.com)
- This product was changed from ascites to tissue culture supernatant on 12th February 2018. (abcam.com)
- He was the recipient of the 1912 Nobel Prize in Physiology or Medicine for his development of surgical techniques to repair blood vessels. (asu.edu)
- Play media While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc. (wikipedia.org)
- The immediate impact on biological research was significant, and widespread application of the technique continues to the present day. (microscopyu.com)
- Learns the biological role of cellular components and their differences in tissues. (atilim.edu.tr)
- Due to the increasing ease by which such powerful tools are available, life scientists and bioengineers have started to use MEMS as a platform for cell culture in order to better answer some fundamental biological questions [ 13 ]. (mdpi.com)
- In addition, GCV prevented growth of multipotent neurospheres from transgenic astrocyte-enriched cell cultures derived from postnatal GZ, and elaidic acid GCV given for 4 d to adult transgenic mice in vivo abolished the ability to grow multipotent neurospheres from GZ. (jneurosci.org)
- A drop of mineral oil is placed on either side of the culture drop which is covered with a coverslip (Fig. (iii) Transplantation: The shoots or plantlets multiplied on a medium containing organic nutrients, show poor photosynthetic capability. (reviewsic.com)
- If one desires to obtain large number of plantlets from the callus, he sub-cultures the callus. (preservearticles.com)
- Some of the applications include the processing of food, pharmaceuticals, diagnostic kits, restoration of water damaged documents, river bottom sludge prepared for hydrocarbon analysis, ceramics used in the semiconductor industry, viral or bacterial cultures, tissues prepared for analysis, the production of synthetic skins and restoration of historic/reclaimed boat hulls. (absoluteastronomy.com)
- The "golden period" is the first 6 to 8 hours between wound contamination at injury and bacterial multiplication to greater than 10 5 CFU per gram of tissue. (vin.com)
- A wound is classified as infected rather than contaminated when bacterial numbers exceed 10 5 CFU per gram of tissue. (vin.com)
- Purification and Characterization of Tissue Plasminogen Activator Kringle-.sup.2 Domain Expressed in Escherichia coli", Biochem. (patentgenius.com)
- The culture vessels used for plant tissue studies includes Erylenmayer flask (conical flask), petri plates and culture tubes (25 x 150mm). (brainkart.com)
- 2 Sterile Pasteur pipets 37 C warming tray or incubator Tissue culture plasticware or glassware including pipets and 25-cm 2 flasks or 60-mm petri plates, sterile NOTE: All culture incubations should be performed in a humidified 37 C, 5% CO 2 incubator unless otherwise specified. (docplayer.net)
- 8,25 Although the lignan production on callus culture have been investigated successfully on species as Podophyllum peltatum 14 , P. hexandrum 10 , Forsythia intermedia 21 and Ipomoea cairica 19 , similar studies have not been reported in R. mucosa so far. (sld.cu)
- Jonathan specialises in conserving endangered plant species from around the world, using tissue culture among other techniques. (saps.org.uk)
- Calli from foliar blade cultured in 10,4, 20,8 and 31,2 µM of picloram presented a two-three fold increase in epiyangambin rates with regard to levels detected in the original plant. (sld.cu)
- The establishment of friable calli cultures also will allow its application in further studies with cell suspension cultures. (sld.cu)
Field of tissue2
- Murashige and Skoog Medium (1965) is the most popular for shoot cultures. (slideserve.com)
- Gamborgs B5 medium is widely used for cell suspension cultures (no ammonium). (slideserve.com)
- It has a small outer chamber (to be filled with sterile distilled water) and a large inner chamber with a number of micro-wells (Fig. Cell suspensions are maintained by routine sub-culturing in a fresh medium. (reviewsic.com)
- Thus there is no 'ideal' medium, but, however, the typical medium used in plant tissue culture technology is Murashige-Scoog (MS) medium. (preservearticles.com)
- This is necessary because the culture medium on which these tissue pieces are inoculated contains mineral salts, vitamins, sugars, phytohor-mones and other growth regulators on which microbial contaminants would grow at a faster rate than plant tissue. (preservearticles.com)
- Conceptually, they coined the term "tissue culture" and gave its definition as "a plasmatic medium inoculated with small fragments of living tissue. (asu.edu)
- Although the method they used was generally based on Harrison's original design, Carrel and Burrows substituted blood plasma for lymph in the tissue culture medium . (asu.edu)
- Another important modification was the addition of embryo extracts to the culture medium . (asu.edu)
- The culture medium is closed with cotton plug/ or aluminium foil sheet. (brainkart.com)
- So, it is important to sterilize the culture medium and plant tissue. (brainkart.com)
- The culture medium can be sterilised by keeping it in an autoclave and maintaining the temperature of 121-C for 15 minutes. (brainkart.com)
- The culture medium with the inoculum is incubated at 26 - 2 o C with the light intensity at 2000 to 4000 lux (unit of intensity of light) and allowing photoperiod of 16 hour of light and 8 hours of darkness. (brainkart.com)
- DMEM) may require altered levels of CO 2 to maintain ph Remove all medium from primary culture with a sterile Pasteur pipet. (docplayer.net)
- If this is the first medium change, rather than discarding medium that is removed from primary culture, put it into a fresh dish or flask. (docplayer.net)
- If cultures are to be split 1/3 or 1/4 rather than 1/2, add sufficient medium such that 1 ml of cell suspension can be transferred into each fresh culture vessel. (docplayer.net)
- There are four stages in the complete drying process: pretreatment, freezing, primary drying, and secondary drying. (absoluteastronomy.com)
- Maintain a monthly freezer inventory of primary and secondary antibodies, seras, toxins and dyes. (academiccareers.com)
- GCV prevented growth of secondary multipotent neurospheres prepared after passage of primary transgenic neurospheres derived from all three of these developmental stages. (jneurosci.org)
- In: Al-Khayri JM, Jain S, Johnson D (eds) Date palm biotechnology protocols, Vol I, Tissue culture applications. (springer.com)
- Investigate factors affecting successful maturation of immature follicles obtained from ovarian tissue including the use of 3-dimensional biogel scaffolds, growth factors, hormones and other culture conditions. (clinicaltrials.gov)
- Plant tissue culture technology bears tremendous potential for the improvement of crops and ornamentals. (preservearticles.com)
- What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. (routledge.com)
- Because of the wide range of applications, plant tissue culture attracts the attention of molecular biologists, plant breeders and industrialists. (reviewsic.com)
- Cell cultures are utilized in cytogenetic, biochemical, and molecular laboratories for diagnostic as well as research studies. (docplayer.net)
- Callus culture is used as the basis for organogenic (shoot, root) cultures, cell cultures or proliferation of embryoids. (fao.org)