Saccharomyces cerevisiae. Medical search

A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.

Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.

Proteins found in any species of fungus.

The functional hereditary units of FUNGI.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.

A genus of ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Deoxyribonucleic acid that makes up the genetic material of fungi.

Structures within the nucleus of fungal cells consisting of or containing DNA, which carry genetic information essential to the cell.

The complete gene complement contained in a set of chromosomes in a fungus.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.

Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.

Reproductive bodies produced by fungi.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

A glycoside hydrolase found primarily in PLANTS and YEASTS. It has specificity for beta-D-fructofuranosides such as SUCROSE.

Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.

Genes that have a suppressor allele or suppressor mutation (SUPPRESSION, GENETIC) which cancels the effect of a previous mutation, enabling the wild-type phenotype to be maintained or partially restored. For example, amber suppressors cancel the effect of an AMBER NONSENSE MUTATION.

The chromosomal constitution of cells, in which each type of CHROMOSOME is represented once. Symbol: N.

Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.

Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.

Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.

Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.

Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.

The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.

Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.

The rate dynamics in chemical or physical systems.

Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)

A set of nuclear proteins in SACCHAROMYCES CEREVISIAE that are required for the transcriptional repression of the silent mating type loci. They mediate the formation of silenced CHROMATIN and repress both transcription and recombination at other loci as well. They are comprised of 4 non-homologous, interacting proteins, Sir1p, Sir2p, Sir3p, and Sir4p. Sir2p, an NAD-dependent HISTONE DEACETYLASE, is the founding member of the family of SIRTUINS.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.

Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.

Fungal genes that mostly encode TRANSCRIPTION FACTORS. In some FUNGI they also encode PHEROMONES and PHEROMONE RECEPTORS. The transcription factors control expression of specific proteins that give a cell its mating identity. Opposite mating type identities are required for mating.

The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.

An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.

Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.

An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.

A protein kinase encoded by the Saccharomyces cerevisiae CDC28 gene and required for progression from the G1 PHASE to the S PHASE in the CELL CYCLE.

Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.

Proteins prepared by recombinant DNA technology.

The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.

Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.

The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.

A steroid of interest both because its biosynthesis in FUNGI is a target of ANTIFUNGAL AGENTS, notably AZOLES, and because when it is present in SKIN of animals, ULTRAVIOLET RAYS break a bond to result in ERGOCALCIFEROL.

A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).

Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Chemical substances, excreted by an organism into the environment, that elicit behavioral or physiological responses from other organisms of the same species. Perception of these chemical signals may be olfactory or by contact.

A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

Transport proteins that carry specific substances in the blood or across cell membranes.

A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.

A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.

Deletion of sequences of nucleic acids from the genetic material of an individual.

Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

A sirtuin family member found primarily in the CYTOPLASM. It is a multifunctional enzyme that contains a NAD-dependent deacetylase activity that is specific for HISTONES and a mono-ADP-ribosyltransferase activity.

Genes whose loss of function or gain of function MUTATION leads to the death of the carrier prior to maturity. They may be essential genes (GENES, ESSENTIAL) required for viability, or genes which cause a block of function of an essential gene at a time when the essential gene function is required for viability.

The process by which a DNA molecule is duplicated.

An enzyme that converts UDP glucosamine into chitin and UDP. EC 2.4.1.16.

Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.

Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.

A carboxypeptidase that catalyzes the release of a C-terminal amino acid with a broad specificity. It also plays a role in the LYSOSOMES by protecting BETA-GALACTOSIDASE and NEURAMINIDASE from degradation. It was formerly classified as EC 3.4.12.1 and EC 3.4.21.13.

A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.

Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.

Any method used for determining the location of and relative distances between genes on a chromosome.

A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.

Fermented juice of fresh grapes or of other fruit or plant products used as a beverage.

Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.

Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.

The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.

A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.

Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.

The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.

The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.

Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.

Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique.

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.

A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.

An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction.

Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.

A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS from SACCHAROMYCES CEREVISIAE. It is involved in morphological events related to the cell cycle. This enzyme was formerly listed as EC 3.6.1.47.

A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.

Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.

A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.

A DNA-binding protein that mediates DNA REPAIR of double strand breaks, and HOMOLOGOUS RECOMBINATION.

The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.

Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.

A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.

A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.

An alkylating agent in cancer therapy that may also act as a mutagen by interfering with and causing damage to DNA.

Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.

The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.

An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.

A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.

A systemic agricultural fungicide used for control of certain fungal diseases of stone fruit.

The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)

A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.

A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.

RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.

Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.

Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.

Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

The sum of the weight of all the atoms in a molecule.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

Presence of warmth or heat or a temperature notably higher than an accustomed norm.

Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.

An enzyme that catalyzes the conversion of alpha,alpha-trehalose and water to D-glucose. EC 3.2.1.28.

The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.

A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)

The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.

The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.

Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.

Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.

Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.

A family of pheromone receptors that were initially discovered in SACCHAROMYCES CEREVISIAE as proteins necessary for fungal conjugation. Each mating factor receptor is expressed in HAPLOID CELLS of a single mating type.

Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.

An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman.

A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.

A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.

Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.

A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.

Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

An enzyme that catalyzes reversibly the formation of galactose 1-phosphate and ADP from ATP and D-galactose. Galactosamine can also act as the acceptor. A deficiency of this enzyme results in GALACTOSEMIA. EC 2.7.1.6.

Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.

Toxic compounds produced by FUNGI.

Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.

The asymmetrical segregation of genes during replication which leads to the production of non-reciprocal recombinant strands and the apparent conversion of one allele into another. Thus, e.g., the meiotic products of an Aa individual may be AAAa or aaaA instead of AAaa, i.e., the A allele has been converted into the a allele or vice versa.

Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.

The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)

Microbodies which occur in animal and plant cells and in certain fungi and protozoa. They contain peroxidase, catalase, and allied enzymes. (From Singleton and Sainsbury, Dictionary of Microbiology and Molecular Biology, 2nd ed)

That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.

Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.

Cellular proteins and protein complexes that transport amino acids across biological membranes.

Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)

A class of enzymes that form a thioester bond to UBIQUITIN with the assistance of UBIQUITIN-ACTIVATING ENZYMES. They transfer ubiquitin to the LYSINE of a substrate protein with the assistance of UBIQUITIN-PROTEIN LIGASES.

Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.

The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.

The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Life or metabolic reactions occurring in an environment containing oxygen.

Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.

A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.

Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.

Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.

A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed)

The reciprocal exchange of segments at corresponding positions along pairs of homologous CHROMOSOMES by symmetrical breakage and crosswise rejoining forming cross-over sites (HOLLIDAY JUNCTIONS) that are resolved during CHROMOSOME SEGREGATION. Crossing-over typically occurs during MEIOSIS but it may also occur in the absence of meiosis, for example, with bacterial chromosomes, organelle chromosomes, or somatic cell nuclear chromosomes.

A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-

Those genes found in an organism which are necessary for its viability and normal function.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.

Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.

A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.

In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.

Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.

Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.

The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.

Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.

Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.

Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. (1/41576)

The product of the oskar gene directs posterior patterning in the Drosophila oocyte, where it must be deployed specifically at the posterior pole. Proper expression relies on the coordinated localization and translational control of the oskar mRNA. Translational repression prior to localization of the transcript is mediated, in part, by the Bruno protein, which binds to discrete sites in the 3' untranslated region of the oskar mRNA. To begin to understand how Bruno acts in translational repression, we performed a yeast two-hybrid screen to identify Bruno-interacting proteins. One interactor, described here, is the product of the apontic gene. Coimmunoprecipitation experiments lend biochemical support to the idea that Bruno and Apontic proteins physically interact in Drosophila. Genetic experiments using mutants defective in apontic and bruno reveal a functional interaction between these genes. Given this interaction, Apontic is likely to act together with Bruno in translational repression of oskar mRNA. Interestingly, Apontic, like Bruno, is an RNA-binding protein and specifically binds certain regions of the oskar mRNA 3' untranslated region. (+info)

Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. (2/41576)

The vacuolar protein sorting (VPS) pathway of Saccharomyces cerevisiae mediates transport of vacuolar protein precursors from the late Golgi to the lysosome-like vacuole. Sorting of some vacuolar proteins occurs via a prevacuolar endosomal compartment and mutations in a subset of VPS genes (the class D VPS genes) interfere with the Golgi-to-endosome transport step. Several of the encoded proteins, including Pep12p/Vps6p (an endosomal target (t) SNARE) and Vps45p (a Sec1p homologue), bind each other directly [1]. Another of these proteins, Vac1p/Pep7p/Vps19p, associates with Pep12p and binds phosphatidylinositol 3-phosphate (PI(3)P), the product of the Vps34 phosphatidylinositol 3-kinase (PI 3-kinase) [1] [2]. Here, we demonstrate that Vac1p genetically and physically interacts with the activated, GTP-bound form of Vps21p, a Rab GTPase that functions in Golgi-to-endosome transport, and with Vps45p. These results implicate Vac1p as an effector of Vps21p and as a novel Sec1p-family-binding protein. We suggest that Vac1p functions as a multivalent adaptor protein that ensures the high fidelity of vesicle docking and fusion by integrating both phosphoinositide (Vps34p) and GTPase (Vps21p) signals, which are essential for Pep12p- and Vps45p-dependent targeting of Golgi-derived vesicles to the prevacuolar endosome. (+info)

The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. (3/41576)

Polarized secretion requires proper targeting of secretory vesicles to specific sites on the plasma membrane. Here we report that the exocyst complex plays a key role in vesicle targeting. Sec15p, an exocyst component, can associate with secretory vesicles and interact specifically with the rab GTPase, Sec4p, in its GTP-bound form. A chain of protein-protein interactions leads from Sec4p and Sec15p on the vesicle, through various subunits of the exocyst, to Sec3p, which marks the sites of exocytosis on the plasma membrane. Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion. (+info)

Cooperative binding of heat shock factor to the yeast HSP82 promoter in vivo and in vitro. (4/41576)

Previous work has shown that heat shock factor (HSF) plays a central role in remodeling the chromatin structure of the yeast HSP82 promoter via constitutive interactions with its high-affinity binding site, heat shock element 1 (HSE1). The HSF-HSE1 interaction is also critical for stimulating both basal (noninduced) and induced transcription. By contrast, the function of the adjacent, inducibly occupied HSE2 and -3 is unknown. In this study, we examined the consequences of mutations in HSE1, HSE2, and HSE3 on HSF binding and transactivation. We provide evidence that in vivo, HSF binds to these three sites cooperatively. This cooperativity is seen both before and after heat shock, is required for full inducibility, and can be recapitulated in vitro on both linear and supercoiled templates. Quantitative in vitro footprinting reveals that occupancy of HSE2 and -3 by Saccharomyces cerevisiae HSF (ScHSF) is enhanced approximately 100-fold through cooperative interactions with the HSF-HSE1 complex. HSE1 point mutants, whose basal transcription is virtually abolished, are functionally compensated by cooperative interactions with HSE2 and -3 following heat shock, resulting in robust inducibility. Using a competition binding assay, we show that the affinity of recombinant HSF for the full-length HSP82 promoter is reduced nearly an order of magnitude by a single-point mutation within HSE1, paralleling the effect of these mutations on noninduced transcript levels. We propose that the remodeled chromatin phenotype previously shown for HSE1 point mutants (and lost in HSE1 deletion mutants) stems from the retention of productive, cooperative interactions between HSF and its target binding sites. (+info)

Correlation between protein and mRNA abundance in yeast. (5/41576)

We have determined the relationship between mRNA and protein expression levels for selected genes expressed in the yeast Saccharomyces cerevisiae growing at mid-log phase. The proteins contained in total yeast cell lysate were separated by high-resolution two-dimensional (2D) gel electrophoresis. Over 150 protein spots were excised and identified by capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein spots were quantified by metabolic labeling and scintillation counting. Corresponding mRNA levels were calculated from serial analysis of gene expression (SAGE) frequency tables (V. E. Velculescu, L. Zhang, W. Zhou, J. Vogelstein, M. A. Basrai, D. E. Bassett, Jr., P. Hieter, B. Vogelstein, and K. W. Kinzler, Cell 88:243-251, 1997). We found that the correlation between mRNA and protein levels was insufficient to predict protein expression levels from quantitative mRNA data. Indeed, for some genes, while the mRNA levels were of the same value the protein levels varied by more than 20-fold. Conversely, invariant steady-state levels of certain proteins were observed with respective mRNA transcript levels that varied by as much as 30-fold. Another interesting observation is that codon bias is not a predictor of either protein or mRNA levels. Our results clearly delineate the technical boundaries of current approaches for quantitative analysis of protein expression and reveal that simple deduction from mRNA transcript analysis is insufficient. (+info)

The Saccharomyces cerevisiae ETH1 gene, an inducible homolog of exonuclease III that provides resistance to DNA-damaging agents and limits spontaneous mutagenesis. (6/41576)

The recently sequenced Saccharomyces cerevisiae genome was searched for a gene with homology to the gene encoding the major human AP endonuclease, a component of the highly conserved DNA base excision repair pathway. An open reading frame was found to encode a putative protein (34% identical to the Schizosaccharomyces pombe eth1(+) [open reading frame SPBC3D6.10] gene product) with a 347-residue segment homologous to the exonuclease III family of AP endonucleases. Synthesis of mRNA from ETH1 in wild-type cells was induced sixfold relative to that in untreated cells after exposure to the alkylating agent methyl methanesulfonate (MMS). To investigate the function of ETH1, deletions of the open reading frame were made in a wild-type strain and a strain deficient in the known yeast AP endonuclease encoded by APN1. eth1 strains were not more sensitive to killing by MMS, hydrogen peroxide, or phleomycin D1, whereas apn1 strains were approximately 3-fold more sensitive to MMS and approximately 10-fold more sensitive to hydrogen peroxide than was the wild type. Double-mutant strains (apn1 eth1) were approximately 15-fold more sensitive to MMS and approximately 2- to 3-fold more sensitive to hydrogen peroxide and phleomycin D1 than were apn1 strains. Elimination of ETH1 in apn1 strains also increased spontaneous mutation rates 9- or 31-fold compared to the wild type as determined by reversion to adenine or lysine prototrophy, respectively. Transformation of apn1 eth1 cells with an expression vector containing ETH1 reversed the hypersensitivity to MMS and limited the rate of spontaneous mutagenesis. Expression of ETH1 in a dut-1 xthA3 Escherichia coli strain demonstrated that the gene product functionally complements the missing AP endonuclease activity. Thus, in apn1 cells where the major AP endonuclease activity is missing, ETH1 offers an alternate capacity for repair of spontaneous or induced damage to DNA that is normally repaired by Apn1 protein. (+info)

The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae. (7/41576)

Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5'-->3' Exo1 and the 3'-->5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) mutants defective in 3'-->5' proofreading exonuclease, mutants defective in the 5'-->3' exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes. (+info)

A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. (8/41576)

The assembly pathway of spliceosomal snRNPs in yeast is poorly understood. We devised a screen to identify mutations blocking the assembly of newly synthesized U4 snRNA into a functional snRNP. Fifteen mutant strains failing either to accumulate the newly synthesized U4 snRNA or to assemble a U4/U6 particle were identified and categorized into 13 complementation groups. Thirteen previously identified splicing-defective prp mutants were also assayed for U4 snRNP assembly defects. Mutations in the U4/U6 snRNP components Prp3p, Prp4p, and Prp24p led to disassembly of the U4/U6 snRNP particle and degradation of the U6 snRNA, while prp17-1 and prp19-1 strains accumulated free U4 and U6 snRNA. A detailed analysis of a newly identified mutant, the sad1-1 mutant, is presented. In addition to having the snRNP assembly defect, the sad1-1 mutant is severely impaired in splicing at the restrictive temperature: the RP29 pre-mRNA strongly accumulates and splicing-dependent production of beta-galactosidase from reporter constructs is abolished, while extracts prepared from sad1-1 strains fail to splice pre-mRNA substrates in vitro. The sad1-1 mutant is the only splicing-defective mutant analyzed whose mutation preferentially affects assembly of newly synthesized U4 snRNA into the U4/U6 particle. SAD1 encodes a novel protein of 52 kDa which is essential for cell viability. Sad1p localizes to the nucleus and is not stably associated with any of the U snRNAs. Sad1p contains a putative zinc finger and is phylogenetically highly conserved, with homologues identified in human, Caenorhabditis elegans, Arabidospis, and Drosophila. (+info)

... is used as a probiotic in humans and animals. The strain Saccharomyces cerevisiae var. boulardii is ... where Saccharomyces cerevisiae becomes dormant. A variant yeast known as Saccharomyces cerevisiae var. diastaticus is a beer ... Cases of infection of oral cavity and pharynx caused by S. cerevisiae are also known. Occasionally Saccharomyces cerevisiae ... Wikimedia Commons has media related to Saccharomyces cerevisiae. Saccharomyces Genome Database Yeast Resource Center Public ...

https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae

"Correlation between Saccharomyces cerevisiae DNA in intestinal mucosal samples and anti-Saccharomyces cerevisiae antibodies in ... Anti-Saccharomyces cerevisiae antibodies (ASCAs) are antibodies against antigens presented by the cell wall of the yeast ... 2004). "Anti-Saccharomyces cerevisiae antibodies (ASCA) in Crohn's disease are associated with disease severity but not NOD2/ ... 2005). "Anti-Saccharomyces cerevisiae antibodies (ASCA) in Behçet's syndrome". Clin. Exp. Rheumatol. 23 (4 Suppl 38): S67-70. ...

https://en.wikipedia.org/wiki/Anti–Saccharomyces_cerevisiae_antibody

"Saccharomyces cerevisiae killer virus"), known to encode the killer toxin in many S. cerevisiae strains which confers the ... Saccharomyces cerevisiae virus L-A, also called L-A helper virus, is a member of the Totiviridae family of viruses found ... primarily in Saccharomyces cerevisiae. Its discovery in the 1970s was the main starting point of research on yeast virology. It ...

https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_virus_L-A

An endonuclease from organism Saccharomyces cerevisiae, Mus81-Mms4, has been found to interact with a protein labeled Crp1 that ... Rass U, Kemper B (November 2002). "Crp1p, a new cruciform DNA-binding protein in the yeast Saccharomyces cerevisiae". Journal ... Phung HT, Tran DH, Nguyen TX (September 2020). "Saccharomyces cerevisiae". FEBS Letters. 594 (24): 4320-4337. doi:10.1002/1873- ... Crp1 was separately identified as a cruciform-binding protein in S. cerevisiae because it had a high affinity to target ...

https://en.wikipedia.org/wiki/Cruciform_DNA

Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae. Microbiology 142, 1385-1390. Mollapour, M., ... Saccharomyces cerevisiae). Hence, it is conceivable that Z. bailii puts more effort on limiting the influx of acids in order to ... The higher resistance of Z. bailii to weak acids than S. cerevisiae can partly be explained by its ability to metabolize ... Mechanism of resistance of Saccharomyces bailii to benzoic, sorbic and other weak acids used as food preservatives. Journal of ...

https://en.wikipedia.org/wiki/Zygosaccharomyces_bailii

... is a cytosolic protein found in yeast (Saccharomyces cerevisiae) which plays a role in the regulation of several cellular ... CRAL-TRIO Domain Sha B, Phillips SE, Bankaitis VA, Luo M (January 1998). "Crystal structure of the Saccharomyces cerevisiae ... Saccharomyces cerevisiae". Biochimica et Biophysica Acta (BBA) - Biomembranes. 986 (2): 301-9. doi:10.1016/0005-2736(89)90481-1 ... are required for Cvt vesicle and autophagosome assembly in Saccharomyces cerevisiae". Molecular Biology of the Cell. 15 (5): ...

https://en.wikipedia.org/wiki/Sec14

"Saccharomyces cerevisiae S288c CHA1". biocyc.org. Retrieved 2018-12-23. "Saccharomyces cerevisiae CHA1". pathway.yeastgenome. ... YCL064C is a gene in the genome of Saccharomyces cerevisiae, the most common species of yeast. Its function is the catalyzation ... Orphaned articles from September 2019, All orphaned articles, Saccharomyces cerevisiae genes). ...

https://en.wikipedia.org/wiki/YCL064C

... is the Saccharomyces cerevisiae (a yeast) eukaryotic translation release factor. More specifically, it is the yeast ... "Sup35p [Saccharomyces cerevisiae]". National Center for Biotechnology Information, U.S. National Library of Medicine. Paushkin ...

https://en.wikipedia.org/wiki/Sup35p

Saccharomyces Saccharomyces acidi-lactici Grotenf. Saccharomyces cerevisiae Hansen. Saccharomyces fragilis Jorg. Saccharomyces ...

https://en.wikipedia.org/wiki/List_of_fungi_of_South_Africa_–_S

Saccharomyces cerevisiae ferments carbohydrates into carbon dioxide and alcohol, and is used in the making of beer and bread. S ... "Saccharomyces cerevisiae - MicrobeWiki". MicrobeWiki. Retrieved 2015-11-23. "Using yeast in biology". www.yourgenome.org. ... Furthermore, research using S. cerevisiae has played a central role in understanding the mechanism of meiotic recombination and ... cerevisiae is also an important model organism, since it is a eukaryotic organism that's easy to grow. It has been used to ...

https://en.wikipedia.org/wiki/Unicellular_organism

"SYP1 [Saccharomyces cerevisiae]". ncbi.nlm.nih.gov/. ncbi.nlm.nih.gov/. Retrieved 26 May 2017. Boettner DR, D'Agostino JL, ...

https://en.wikipedia.org/wiki/Muniscins

"MCR1 Mcr1p [Saccharomyces cerevisiae]". Entrez Gene. National Center for Biotechnology Information. (Saccharomyces cerevisiae ...

https://en.wikipedia.org/wiki/Mcr1

The Ty5 is a type of retrotransposon native to the Saccharomyces cerevisiae organism. Ty5 is one of five endogenous ... Pryde FE, Louis EJ (November 1997). "Saccharomyces cerevisiae telomeres. A review". Biochemistry Mosc. 62 (11): 1232-41. PMID ... "Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae". Genetics. 116 (1): 9- ... a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence". Genome Res. 8 (5 ...

https://en.wikipedia.org/wiki/Ty5_retrotransposon

"Epigenetics in Saccharomyces cerevisiae." Epigenetics. 1. Cold Spring Harbor Press, 2007. Morgan, David O. (2007). The Cell ... S. cerevisiae has 16 chromosomes, S. pombe has 3. S. cerevisiae is often diploid while S. pombe is usually haploid. S. pombe ... Conversely, S. cerevisiae has well-developed peroxisomes, while S. pombe does not. S. cerevisiae has small point centromere of ... The yeast species Schizosaccharomyces pombe and Saccharomyces cerevisiae are both extensively studied; these two species ...

https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe

Zymolectins in Saccharomyces cerevisiae. Letter J. Inst. Brew., 104:298. (section 4.1) (El-Behhari et al., 2000) (Masy et al., ... In the case of "top-fermenting" ale yeast (Saccharomyces cerevisiae), the yeast creates a krausen, or barm on the top of the ... liquid, unlike "bottom-fermenting" lager yeast (Saccharomyces pastorianus) where the yeast falls to the bottom of the brewing ...

https://en.wikipedia.org/wiki/Yeast_flocculation

Jin, Y-L.; Speers, R.A.. (1999). "Flocculation in Saccharomyces cerevisiae". Food Res. Int. 31 (6-7): 421-440. doi:10.1016/ ...

https://en.wikipedia.org/wiki/Flocculation

Saccharomyces cerevisiae (S. cerevisiae) is the only known eukaryote with a defined initiation sequence TTTTTATG/ATTTA/T. This ... Bell SP, Labib K (July 2016). "Chromosome Duplication in Saccharomyces cerevisiae". Genetics. 203 (3): 1027-1067. doi:10.1534/ ... In S. cerevisiae, CDKs prevent formation of the replication complex during late G1, S, and G2 phases by excluding MCM2-7 and ... initiation sequence is recognized by ORC1-5. ORC6 is not known to bind DNA in S. cerevisiae. Initiation sequences in S. pombe ...

https://en.wikipedia.org/wiki/Pre-replication_complex

Kunz BA, Ramachandran K, Vonarx EJ (April 1998). "DNA sequence analysis of spontaneous mutagenesis in Saccharomyces cerevisiae ... Heidenreich, Erich (January 2007). "Adaptive Mutation in Saccharomyces cerevisiae". Critical Reviews in Biochemistry and ... recombination has been reported to be involved in acquisition of resistance to 5-fluorocytosine in Saccharomyces cerevisiae, ... genome duplications have been found to confer resistance in S. cerevisiae to nutrient-poor environments. In the laboratory, ...

https://en.wikipedia.org/wiki/Mutagenesis

"PNP Oxidase from Saccharomyces Cerevisiae". doi:10.2210/pdb1ci0/pdb. {{cite journal}}: Cite journal requires ,journal= (help) ...

https://en.wikipedia.org/wiki/Odorant-binding_protein

Saccharomyces cerevisiae is generally used. These systems can check for forward and reverse mutations, as well as recombinant ...

https://en.wikipedia.org/wiki/Mutagen

Saccharomyces cerevisiae. S. cerevisiae without this factor, known as the ρ-factor, is described by the development of small ... petite (ρ-) is a mutant first discovered in the yeast Saccharomyces cerevisiae. Due to the defect in the respiratory chain, ' ... A mutation that produces small (petite" > petite) anaerobic-like colonies had shown first in Yeast Saccharomyces cerevisiae and ... mutation by chemical and physical agents in Saccharomyces cerevisiae". Mutation Research. 265 (1): 103-148. doi:10.1016/0027- ...

https://en.wikipedia.org/wiki/Petite_mutation

Clarke CF, Williams W, Teruya JH (1991). "Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, ...

https://en.wikipedia.org/wiki/Hexaprenyldihydroxybenzoate_methyltransferase

The yeast species Saccharomyces cerevisiae is an important model organism in cell biology. The fruiting bodies of some larger ... The yeast species Saccharomyces cerevisiae has been an important model organism in modern cell biology for much of the ... Legras JL, Merdinoglu D, Cornuet JM, Karst F (2007). "Bread, beer and wine: Saccharomyces cerevisiae diversity reflects human ... Ostergaard S, Olsson L, Nielsen J (2000). "Metabolic Engineering of Saccharomyces cerevisiae". Microbiology and Molecular ...

https://en.wikipedia.org/wiki/Human_interactions_with_fungi

"Phosphate permeases of Saccharomyces cerevisiae". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1365 (1-2): 23-30. doi: ... "Phosphate permeases of Saccharomyces cerevisiae: structure, function and regulation". Biochimica et Biophysica Acta (BBA) - ...

https://en.wikipedia.org/wiki/Inorganic_phosphate_transporter_family

Saccharomyces cerevisiae (top-fermenting yeast) and S. carlsbergensis (bottom-fermenting yeast). S. cerevisiae has been sold ... The budding yeast Saccharomyces cerevisiae reproduces by mitosis as diploid cells when nutrients are abundant, but when starved ... The yeast species Saccharomyces cerevisiae converts carbohydrates to carbon dioxide and alcohols through the process of ... The term "yeast" is often taken as a synonym for Saccharomyces cerevisiae, but the phylogenetic diversity of yeasts is shown by ...

https://en.wikipedia.org/wiki/Yeast

Top-fermented beers are most commonly produced with Saccharomyces cerevisiae, a top-fermenting yeast which clumps and rises to ... Many of these are not strains of brewer's yeast (Saccharomyces cerevisiae) and may have significant differences in aroma and ... The dominant types of yeast used to make beer are the top-fermenting Saccharomyces cerevisiae and bottom-fermenting ... Ostergaard, Simon; Olsson, Lisbeth; Nielsen, Jens (1 March 2000). "Metabolic Engineering of Saccharomyces cerevisiae". ...

https://en.wikipedia.org/wiki/Beer

Santangelo, G. M. (March 2006). "Glucose Signaling in Saccharomyces cerevisiae". Microbiology and Molecular Biology Reviews. 70 ...

https://en.wikipedia.org/wiki/George_Santangelo

"Processing glycosidases of Saccharomyces cerevisiae." Biochimica et Biophysica Acta (BBA) - General Subjects 1426.2 (1999): 275 ...

https://en.wikipedia.org/wiki/Annette_Herscovics

In Saccharomyces cerevisiae, the 5S rDNA has the unusual feature of lying inside the rDNA repeat. It is flanked by non- ... Venema, Jaap; Tollervey, David (December 1999). "Ribosome Synthesis in Saccharomyces cerevisiae". Annual Review of Genetics. 33 ... and its crystal structure in the yeast Saccharomyces cerevisiae was solved at 2.8Å resolution in 2013. Twelve of its subunits ...

https://en.wikipedia.org/wiki/RNA_polymerase_I

Many of the relevant genes were first identified by studying yeast, especially Saccharomyces cerevisiae; genetic nomenclature ... Several gene expression studies in Saccharomyces cerevisiae have identified 800-1200 genes that change expression over the ... Analyses of synchronized cultures of Saccharomyces cerevisiae under conditions that prevent DNA replication initiation without ... December 1998). "Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray ...

https://en.wikipedia.org/wiki/Cell_cycle

... namely Asp91 in Saccharomyces cerevisiae). Repulsion between the negative charges would raise the energy value near the ... Nonetheless, crystallographic analyses and the lack of S. cerevisiae enzyme affinity to substrate analogues where the ...

https://en.wikipedia.org/wiki/Uridine_monophosphate_synthase

"Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review". Lipids. 30 (3): 221-6 ...

https://en.wikipedia.org/wiki/Metabolism

... A from Saccharomyces cerevisiae Merck KGaA Zymosan at the US National Library of Medicine Medical Subject Headings ( ...

https://en.wikipedia.org/wiki/Zymosan

Recent evidence of crystal structures of proteasomes isolated from Saccharomyces cerevisiae suggests that the catalytically ... "Dissection of the assembly pathway of the proteasome lid in Saccharomyces cerevisiae". Biochemical and Biophysical Research ...

https://en.wikipedia.org/wiki/PSMD7

... associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae". Mol. Cell. Biol. 16 (7): ...

https://en.wikipedia.org/wiki/ABL_(gene)

Ryley J, Pereira-Smith OM (2006). "Microfluidics device for single cell gene expression analysis in Saccharomyces cerevisiae". ... ranging from the simple budding yeast Saccharomyces cerevisiae to worms such as Caenorhabditis elegans and fruit flies ( ...

https://en.wikipedia.org/wiki/Senescence

... is a database of transcription factors for Saccharomyces cerevisiae. Transcription factor de Boer, Carl G; Hughes Timothy R ( ...

https://en.wikipedia.org/wiki/YeTFaSCo

It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and ... "Entrez Gene: RAD54B RAD54 homolog B (S. cerevisiae)". McAndrew EN, Lepage CC, McManus KJ (December 2016). "The synthetic lethal ...

https://en.wikipedia.org/wiki/RAD54B

... a Saccharomyces cerevisiae transposon family. Ty the Tasmanian Tiger 2: Bush Rescue, a video game Ty21a This disambiguation ...

https://en.wikipedia.org/wiki/Ty2

Yates in which they used shotgun proteomics on the proteome of a Saccharomyces cerevisiae strain grown to mid-log phase. They ...

https://en.wikipedia.org/wiki/Shotgun_proteomics

"Candida zemplinina can reduce acetic acid produced by Saccharomyces cerevisiae in sweet wine fermentations". Applied and ... "Anaerobic organic acid metabolism of Candida zemplinina in comparison with Saccharomyces wine yeasts". International Journal of ...

https://en.wikipedia.org/wiki/Candida_zemplinina

... farnesene is produced from fermentation of sugarcane sugars using genetically modified Saccharomyces cerevisiae yeast strains. ...

https://en.wikipedia.org/wiki/Squalane

... saccharomyces MeSH B05.107.795.785.800 - saccharomyces cerevisiae MeSH B05.107.795.815 - saccharomycopsis MeSH B05.107.795.980 ... saccharomyces MeSH B05.930.705.655 - saccharomyces cerevisiae MeSH B05.930.710 - saccharomycopsis MeSH B05.930.720 - ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(B05)

Iwahashi Y, Hitoshio A, Tajima N, Nakamura T (Apr 1989). "Characterization of NADH kinase from Saccharomyces cerevisiae". ...

https://en.wikipedia.org/wiki/NAD _kinase

This system uses Saccharomyces cerevisiae with transgenes from Papaver somniferum (the opium poppy) and Pseudomonas putida to ...

https://en.wikipedia.org/wiki/Oxycodone

... cloned the CDC28 gene from the budding yeast Saccharomyces cerevisiae. As a group leader in Cambridge Nasmyth became interested ... Together with Kelly Tatchell he cloned the S. cerevisiae mating-type locus and found, surprisingly, that 'silent' copies of the ... Cerevisiae". Cell. 79 (2): 233-44. doi:10.1016/0092-8674(94)90193-7. PMID 7954792. S2CID 34939988. "Jan Nasmyth". Daily ...

https://en.wikipedia.org/wiki/Kim_Nasmyth

Klar, Amar (1975). Enzyme regulation during vegetative growth and aporulation in Saccharomyces cerevisiae: galactose catabolic ...

https://en.wikipedia.org/wiki/Amar_Klar

However, on 29 May 2013 Oregon officially designated Saccharomyces cerevisiae as the official microbe of the state, making it ...

https://en.wikipedia.org/wiki/Flavobacterium_akiainvivens

October 2001). "Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisiae ... cell type-specific sterile genes from Saccharomyces cerevisiae". EMBO J. 4 (10): 2643-2648. doi:10.1002/j.1460-2075.1985. ... Herskowitz I, Marsh L (1988). "STE2 protein of Saccharomyces kluyveri is a member of the rhodopsin/beta-adrenergic receptor ...

https://en.wikipedia.org/wiki/Fungal_mating_pheromone_receptors

Saccharomyces cerevisiae, and Cryptococcus neoformans. Histatins also precipitate tannins from solution, thus preventing ...

https://en.wikipedia.org/wiki/Histatin

His doctoral research involved the characterization of the first aging genes in the budding yeast, Saccharomyces cerevisiae. In ... Saccharomyces cerevisiae, as a model organism. Because of the pandemic, the lab has pivoted to developing a yeast based ...

https://en.wikipedia.org/wiki/Nicanor_Austriaco

... bond formation and elucidated how disulfide bond formation controls the nuclear localization of the Saccharomyces cerevisiae ...

https://en.wikipedia.org/wiki/Gisela_Storz

"Mutational analysis of NHAoc/NHA2 in Saccharomyces cerevisiae". Biochimica et Biophysica Acta (BBA) - General Subjects. 1800 ( ...

https://en.wikipedia.org/wiki/SLC9B2

Saccharomyces cerevisiae (yeast), Zea mays (maize), barley, Drosophila melanogaster, C. elegans and rat. The origins and ...

https://en.wikipedia.org/wiki/Recombinant_inbred_strain

Gimble FS, Thorner J (May 1992). "Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae". ... "Evidence for translated intervening sequences in the mitochondrial genome of Saccharomyces cerevisiae". J Biol Chem. 257 (6): ... translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae". J Biol Chem. 265 (12): 6726-33. ... Szczepanek T, Lazowska J (July 1996). "Replacement of two non-adjacent amino acids in the S.cerevisiae bi2 intron-encoded RNA ...

https://en.wikipedia.org/wiki/List_of_homing_endonuclease_cutting_sites

"The predominant protein-arginine methyltransferase from Saccharomyces cerevisiae". J. Biol. Chem. 271 (21): 12585-94. doi: ... contribute to cohesin-dependent double-strand break repair by sister chromatid recombination in Saccharomyces cerevisiae". ...

https://en.wikipedia.org/wiki/Histone_methyltransferase

"Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces ... "Entrez Gene: DBR1 debranching enzyme homolog 1 (S. cerevisiae)". Chapman KB, Boeke JD (1991). "Isolation and characterization ...

https://en.wikipedia.org/wiki/DBR1

Saccharomyces cerevisiae), and dried leaves of gesho (Rhamnus prinoides) that serve as a catalyser. The brew is allowed to ...

https://en.wikipedia.org/wiki/Dogu'a_Tembien

"Functional expression of human methionine aminopeptidase type 1 in Saccharomyces cerevisiae". Protein Pept Lett. 9 (4): 295-303 ...

https://en.wikipedia.org/wiki/METAP1

Saccharomyces cerevisiae,/i, and other fungi. Amino acid homeostasis is essential for cell growth and survival. Hence, the ,i, ... Regulation of Amino Acid Transport in Saccharomyces cerevisiae Microbiol Mol Biol Rev. 2019 Oct 16;83(4):e00024-19. doi: ... We review the mechanisms responsible for amino acid homeostasis in Saccharomyces cerevisiae and other fungi. Amino acid ... Keywords: Saccharomyces cerevisiae; amino acid homeostasis; membrane partitioning; plasma membrane; regulation of transport; ...

https://pubmed.ncbi.nlm.nih.gov/31619504/

... GMEU-PD-0060 £9.95 ... All about Beer & Bread (Saccharomyces cerevisiae). FACTS: For at least six thousand years, Saccharomyces cerevisiae has been ... Saccharomyces cervisiae is a fungus known as Bakers & Brewers yeast because its used to make bread and ferment alcoholic ... In 1996, S. cerevisiae was the first eukaryotic organism to have its genome completely sequenced.. ...

https://www.giantmicrobes.com/uk/products/beerandbread.html

Saccharomyces cerevisiae S288C). Find diseases associated with this biological target and compounds tested against it in ...

https://pubchem.ncbi.nlm.nih.gov/gene/APC1/Saccharomyces_cerevisiae_S288C

Saccharomyces cerevisiae Hansen, teleomorph Synonyms. Saccharomyces anamensisWill et Heinrich; Saccharomyces hienipiensis Santa ... Saccharomyces chevalieri Guilliermond; Saccharomyces gaditensis Santa Maria; Saccharomyces cordubensisSanta Maria;Saccharomyces ... hara; Saccharomyces batatae Saito; Saccharomyces aceti Santa Maria; Saccharomyces capensis van der Walt et Tscheuschner; ... To download a certificate of origin for Saccharomyces cerevisiae Meyen ex E.C. Hansen (4109), enter the lot number exactly as ...

https://www.atcc.org/products/4109

Crystal structure of Mxr1 from Saccharomyces cerevisiae in unusual oxidized form ... Crystal structure of Mxr1 from Saccharomyces cerevisiae in unusual oxidized form. *PDB DOI: 10.2210/pdb3PIM/pdb ...

https://www.rcsb.org/structure/3pim

Timeline for Species Bakers yeast (Saccharomyces cerevisiae) [TaxId:4932] from b.33.1.1 ISP subunit of the mitochondrial ... PDB entries in Species: Bakers yeast (Saccharomyces cerevisiae):. *Domain(s) for 1ezv: *. Domain d1ezve1: 1ezv E:87-215 [59548 ... Lineage for Species: Bakers yeast (Saccharomyces cerevisiae). *Root: SCOP 1.61 *. Class b: All beta proteins [48724] (111 ... Species Bakers yeast (Saccharomyces cerevisiae) [TaxId:4932] from b.33.1.1 ISP subunit of the mitochondrial cytochrome bc1- ...

https://scop.berkeley.edu/sunid=63741&ver=1.61

Saccharomyces cerevisiae HG1.1, and the expression of genes during ethanol fermentation at 40 °C were carried out. ... cerevisiae HG1.1 are associated with genes responsible for growth and ethanol stress, oxidative stress, acetic acid stress ... Izmirlioglu, G. & Demirci, A. Ethanol production from waste potato mash by using Saccharomyces cerevisiae. Appl. Sci. 2, 738- ... Down-regulation of the ADH2 gene has been reported in S. cerevisiae KKU-VN8 under heat stress at 40°C34. In S. cerevisiae Y- ...

https://www.nature.com/articles/s41598-022-18212-w?error=cookies_not_supported&code=21809f71-4e1a-4b08-bd8d-458f46a4225f

The yeast Saccharomyces cerevisiae is widely used for the commercial production of bioethanol from sucrose or starch-derived ... While glucose and other hexose sugars like galactose and mannose can be fermented to ethanol by S. cerevisiae, the major ... of functional routes for the conversion of xylose and arabinose to xylulose or xylulose-5-phosphate in Saccharomyces cerevisiae ... Recombinant Diploid Saccharomyces cerevisiae Strain Development for Rapid Glucose and Xylose Co-Fermentation. *Tingting Liu, ...

https://www.semanticscholar.org/paper/Bioconversion-of-lignocellulose-derived-sugars-to-Madhavan-Srivastava/336aa5982df993a1e772c4879fa4f688ba6670d2

The characterization of the starter Saccharomyces cerevisiae needs to be performed also taking into account this ability even ... of wines obtained throughout the fermentation of 10 strains of Saccharomyces cerevisiae. In addition, the production of sulphur ... cerevisiae. In addition, the production of sulfur compounds was further evaluated by using a gas-chromatograph coupled with a ... The characterization of the starter Saccharomyces cerevisiae needs to be performed also taking into account this ability even ...

https://www.frontiersin.org/articles/10.3389/fmicb.2016.00243/full

FAP7 ( NM_001180226.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -, NP_010115.1 Saccharomyces cerevisiae S288C nucleoside ... Saccharomyces cerevisiae S288C nucleoside-triphosphatase (FAP7), partial mRNA.. pcDNA3.1-C-(k)DYK or customized vector. 7-9. $ ... The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.. Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP ... The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.. Nature387(6632 Suppl)75-8(1997 May). Jacq C,Alt-M?rbe J, ...

https://www.genscript.com/gene/saccharomyces-cerevisiae/851388/fap7.html

The structure of the peroxisomal catalase A from the budding yeast Saccharomyces cerevisiae, with 515 residues per subunit, has ...

https://research.pasteur.fr/en/publication/structure-of-catalase-a-from-saccharomyces-cerevisiae/

Recombinant Saccharomyces cerevisiae Alpha-galactosidase 1 (MEL1) from Cusabio. Cat Number: CSB-YP356371SAC. USA, UK & Europe ... Recombinant Saccharomyces cerevisiae Alpha-galactosidase 1 (MEL1) , CSB-YP356371SAC Cusabio Saccharomyces cerevisiae ... Recombinant Saccharomyces cerevisiae Saccharopepsin (PEP4) , CSB-EP362092SVG Cusabio Saccharomyces cerevisiae Recombinants. ... Recombinant Saccharomyces cerevisiae Saccharopepsin (PEP4) , CSB-YP362092SVG Cusabio Saccharomyces cerevisiae Recombinants. ...

https://joplink.net/recombinant-saccharomyces-cerevisiae-alpha-galactosidase-1-mel1-csb-yp356371sac/

Effects ofSaccharomyces cerevisiaeorboulardiiyeasts on acute stress induced intestinal dysmotility Academic Article ... The Saccharomyces cerevisiae processing alpha 1,2-mannosidase is localized in the endoplasmic reticulum, independently of known ... The Functional Role of Conserved Acidic Residues of the Qcr7 Protein of the Cytochrome bc1 Complex in Saccharomyces cerevisiae ... Homoserine dehydrogenase from Saccharomyces cerevisiae: kinetic mechanism and stereochemistry of hydride transfer Academic ...

https://experts.mcmaster.ca/display/vocab-mesh-saccharomyces-cerevisiae

Metabolic engineering of yeast (Saccharomyces cerevisiae) with a view to optimising butanol production ... the yeast Saccharomyces cerevisiae has been used to house the Clostridial ABE-butanol pathway. However, butanol yields and ...

https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764711

Membrane Trafficking - Saccharomyces cerevisiae (budding yeast). [ Brite menu , Download htext , Download json , Copy URL , ...

https://www.kegg.jp/brite/sce04131+YLR208W

... ... Molecular mechanism for degradation of transcriptionally stalled RNA polymerase II in the yeast Saccharomyces cerevisiae. ... Molecular mechanism for degradation of transcriptionally stalled RNA polymerase II in the yeast Saccharomyces cerevisiae.. ...

https://edoc.ub.uni-muenchen.de/12174/

... Show full item record ... Yuan , Q & Jäntti , J 2010 , Functional Analysis of Phosphorylation on Saccharomyces cerevisiae Syntaxin 1 Homologues Sso1p ... Functional Analysis of Phosphorylation on Saccharomyces cerevisiae Syntaxin 1 Homologues Sso1p and Sso2p. ...

https://helda.helsinki.fi/handle/10138/165614

Saccharomyces cerevisiae (Sc) strains employed in the study were derived from CEN.PK 113-5D (Mat a ura3-52 HIS3 LEU2 TRP1 MAL2- ... Qiu Z, Deng Z, Tan H, Zhou S, Cao L. Engineering the robustness of Saccharomyces cerevisiae by introducing bifunctional ... Previously, we overexpressed the genes in the GSH biosynthetic pathway in Saccharomyces cerevisiae and observed a concomitant ... Ask M, Mapelli V, Höck H, Olsson L, Bettiga M. Engineering glutathione biosynthesis of Saccharomyces cerevisiae increases ...

https://www.researchsquare.com/article/rs-72759/v1

... and extend them in silico using TF-gene binding motifs and a compendium of large expression data from Saccharomyces cerevisiae ... We applied the LINK and STAR models to all the nodes in the composite Saccharomyces cerevisiae transcriptional regulatory ... Unraveling condition specific gene transcriptional regulatory networks in Saccharomyces cerevisiae. *Hyunsoo Kim1, ... Kim, H., Hu, W. & Kluger, Y. Unraveling condition specific gene transcriptional regulatory networks in Saccharomyces cerevisiae ...

https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-7-165

... Umaiyah, Ade Sri; Chairul; Yenti ... Fermentasi Nira Nipah Skala 50 Liter Menjadi Bioetanol Menggunakan Saccharomyces Cerevisiae. Login ... Preparation stater done by culturing medium Saccharomyces cereviseae as developer on 10% yeast fermentation medium thus able to ...

https://repository.unri.ac.id/handle/123456789/3973

Seminars and Events at the Research Institute of Molecular Pathology (IMP) and Vienna Biocenter (VBC).

https://www.imp.ac.at/calendar/event/global-analysis-of-kinase-and-phosphatase-action-in-peroxisome-biogenesis-in-saccharomyces-cerevisia/

Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S. cerevisiae ... Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae Journal ... Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S. cerevisiae ...

https://research.chalmers.se/en/publication/193866

Müller, Günter; Bandlow, Wolfhard (1991): Two lipid-anchored cAMP-binding proteins in the yeast Saccharomyces cerevisiae are ...

https://epub.ub.uni-muenchen.de/8687/

Saccharomyces Cerevisiae G1 Cyclins Are Differentially Involved in Invasive and Pseudohyphal Growth Independent of the ... Saccharomyces Cerevisiae G1 Cyclins Are Differentially Involved in Invasive and Pseudohyphal Growth Independent of the ... Resources relating to Saccharomyces Cerevisiae G1 Cyclins Are Differentially Involved in Invasive and Pseudohyphal Growth ...

https://www.dechert.com/knowledge/publication/1999/1/saccharomyces-cerevisiae-g1-cyclins-are-differentially-involved.html

Antibody (IgG, IgA, and IgM) to bakers yeast (Saccharomyces cerevisiae), yeast mannan, gliadin, ovalbumin and ... Antibody (IgG, IgA, and IgM) to bakers yeast (Saccharomyces cerevisiae), yeast mannan, gliadin, ovalbumin and ... Saccharomyces cerevisiae) and yeast cell wall mannan. The twins were made up of five pairs concordant and nine pairs discordant ... Saccharomyces cerevisiae) of all antibody types (IgA, IgG, and IgM). In contrast, the response to gliadin, ovalbumin, and ...

https://gut.bmj.com/content/33/7/909?ijkey=9c862890e31316f6e6b2ca292293991b5d59c60b&keytype2=tf_ipsecsha

... from literature and the fermentation-relevant phenotypic characteristics of null mutants described in the Saccharomyces Genome ... genes required for successful and timely completion of wine-like fermentation by Saccharomyces cerevisiae ...

https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-552/figures/1

"Moderate expression of SEC16 increases protein secretion by Saccharomyces cerevisiae",. abstract = "The yeast Saccharomyces ... The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. However, the limited capacity of the ... N2 - The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. However, the limited capacity of ... AB - The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. However, the limited capacity of ...

https://orbit.dtu.dk/en/publications/moderate-expression-of-sec16-increases-protein-secretion-by-sacch

Natural hybrids from Saccharomyces cerevisiae, Saccharomyces bayanus and Saccharomyces kudriavzevii in wine fermentations. FEMS ... Genetic typing of Saccharomyces strains. S. cerevisiae strain typing was performed by RFLP-mtDNA analysis with Alu I ... Selection of Saccharomyces cerevisiae strains applied to the production of Prieto Picudo rosé wines with a different aromatic ... Characterization of Saccharomyces cerevisiae isolated from must of grape grown in an experimental vineyard. J. Appl. Microbiol ...

http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S2224-79042014000200008&lng=pt&nrm=iso&tlng=en

Saccharomyces cerevisiae) Supplementation and Different Slaughter Age on the Performance, Slaughter and Carcass Properties ... The Influence of Dietary Probiotic (Saccharomyces cerevisiae) Supplementation and Different Slaughter Age on the Performance, ... The Influence of Dietary Probiotic (Saccharomyces cerevisiae) Supplementation and Different Slaughter Age on the Performance, ... containing Saccharomyces cerevisiae at 4 x 108 colony forming units/g) on growth, slaughter and carcass characteristics of ...

https://scialert.net/abstract/?doi=ijps.2005.309.316

We report here the analysis of the ability of a Saccharomyces cerevisiae vector containing a transgene encoding CEA (Yeast-CEA ... Analysis of the ability of recombinant yeast (Saccharomyces cerevisiae)vector encoding carcinoembrionic antigen (CEA) to infect ... Saccharomyces cerevisiae)vector encoding carcinoembrionic antigen (CEA) to infect dendritic cells and activate antigen-specific ... These studies provide the rationale for the clinical evaluation of the S. cerevisiae vector containing a transgene encoding CEA ...

https://aacrjournals.org/mct/article/6/11_Supplement/A103/239816/Analysis-of-the-ability-of-recombinant-yeast

In this study, optimization conditions for high-temperature ethanol fermentation of pineapple waste hydrolysate (PWH) using a newly isolated thermotolerant yeast, Saccharomyces cerevisiae HG1.1, and the expression of genes during ethanol fermentation at 40 °C were carried out. (nature.com)
The yeast Saccharomyces cerevisiae is widely used for the commercial production of bioethanol from sucrose or starch-derived glucose. (semanticscholar.org)
The structure of the peroxisomal catalase A from the budding yeast Saccharomyces cerevisiae, with 515 residues per subunit, has been determined and refined to 2.4 A resolution. (pasteur.fr)
As a robust industrial host and key model organism in the study of fundamental biological processes, the yeast Saccharomyces cerevisiae has been used to house the Clostridial ABE-butanol pathway. (bl.uk)
Molecular mechanism for degradation of transcriptionally stalled RNA polymerase II in the yeast Saccharomyces cerevisiae. (uni-muenchen.de)
Karakasili, Eleni (2010): Molecular mechanism for degradation of transcriptionally stalled RNA polymerase II in the yeast Saccharomyces cerevisiae. (uni-muenchen.de)
Here we demonstrate quantitatively that protein complexes in the yeast Saccharomyces cerevisiae are comprised of a core in which subunits are highly coexpressed, display the same deletion phenotype (essential or nonessential), and share identical functional classification and cellular localization. (umn.edu)
Antibody (IgG, IgA, and IgM) to baker's yeast (Saccharomyces cerevisiae), yeast mannan, gliadin, ovalbumin and betalactoglobulin in monozygotic twins with inflammatory bowel disease. (bmj.com)
To assess whether dietary antigens play a role in inflammatory bowel disease, 26 monozygotic twin pairs with inflammatory bowel disease and 52 healthy controls were investigated for serum antibodies (IgA, IgG, IgM) against ovalbumin, betalactoglobulin, gliadin, whole yeast (Saccharomyces cerevisiae) and yeast cell wall mannan. (bmj.com)
Second, twins who had developed Crohn's disease displayed higher antibody titres towards yeast cell wall mannan in particular, but also to whole yeast (Saccharomyces cerevisiae) of all antibody types (IgA, IgG, and IgM). (bmj.com)
The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. (dtu.dk)
Therefore, our research goal is to further elucidate the mechanism for actin cytoskeleton aging biology in a streamlined model organism, budding yeast, Saccharomyces cerevisiae. (columbia.edu)
This thesis aims to explore the use of DNA inversion catalyzed by the serine integrase from the BXB1 mycobacteriophage as a method of gene control in the brewer's yeast Saccharomyces cerevisiae, which is a common strain used in industrial biotechnology. (concordia.ca)
Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae. (rupress.org)
The Kex2 protein of the yeast Saccharomyces cerevisiae is a membrane-bound, Ca2(+)-dependent serine protease that cleaves the precursors of the mating pheromone alpha-factor and the M1 killer toxin at pairs of basic residues during their transport through the secretory pathway. (rupress.org)
We are using the budding yeast Saccharomyces cerevisiae and its relatives as model organisms to understand a variety of evolutionary processes, including questions about (i) mutation rate and spectrum, (ii) epistasis and fitness landscapes, (iii) gene expression evolution, (iv) expression plasticity in environmental adaptations, (v) evolutionary impacts of pleiotropy, (vi) genomic basis of adaptation, and (vii) phenotypic and fitness effects of synonymous mutations. (umich.edu)

In this perspective, principal aim of this experimental research was the evaluation of the volatile profiles, throughout GC/MS technique coupled with solid phase micro extraction, of wines obtained throughout the fermentation of 10 strains of S. cerevisiae . (frontiersin.org)
This study examined the flocculation behavior of two Saccharomyces cerevisiae strains expressing either Flo1 (LCC1209) genotype or NewFlo (LCC125) phenotype in a laminar flow field by measurement of the fundamental flocculation parameter, the orthokinetic capture coefficient. (eurekamag.com)
The aim of this work was to select indigenous Saccharomyces cerevisiae strains based on a combination of genetic and aroma analyses to be used for inoculation in industrial fermentations and produce rosé wine with a different aromatic profile. (scielo.org.za)
A total of 118 indigenous strains of S. cerevisiae and one hybrid strain from five wineries and three different vintages were isolated from spontaneous microfermentations and genetically characterised according to the restriction fragment length polymorphism of their mitochondrial DNA (RFLP-mtDNA). (scielo.org.za)
S. boulardii is different from other strains of S. cerevisiae, commonly known as brewer's yeast and baker's yeast. (medlineplus.gov)
the strains of S. cerevisiae var. (britannica.com)
A survival level above 5 percent, PR-toxin produced mitotic gene conversion in both strains of Saccharomyces-cerevisiae. (cdc.gov)
In this study, bioethanol production from steam-exploded wheat straw using different process configurations was evaluated using two Saccharomyces cerevisiae strains, F12 and Red Star. (chalmers.se)

HMO1 is one of 10 Saccharomyces cerevisiae HMGB proteins, and it is required for normal growth and plasmid maintenance and for regulating the susceptibility of yeast chromatin to nuclease. (lsu.edu)
In addition to Atg proteins, most vesicle transport regulators are also essential for each step of autophagy.The present study showed that one Endoplasmic Reticulum protein in Saccharomyces cerevisiae, Tip20, which controlsGolgi-to-ER retrograde transport, was also required for starvation-induced autophagy under high temperature stress. (who.int)
Through this project, my main aim is to understand more about the potential relationship between TORC2 and Ras-GTPases of Saccharomyces cerevisiae by analyzing the role of Ras proteins in the complexs localization. (berkeley.edu)

In 1996, S. cerevisiae was the first eukaryotic organism to have its genome completely sequenced. (giantmicrobes.com)
This research involved studying mitochondrial DNA stability using Saccharomyces cerevisiae as a model organism. (suny.edu)
Saccharomyces boulardii (S. boulardii) is a type of probiotic ("friendly" organism). (medlineplus.gov)

Saccharomyces cerevisiae S288C nucleoside-triphosphatase (FAP7), partial mRNA. (genscript.com)

Saccharomyces cervisiae is a fungus known as Baker's & Brewer's yeast because it's used to make bread and ferment alcoholic beverages, like beer and wine. (giantmicrobes.com)
A species of the genus SACCHAROMYCES , family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. (bvsalud.org)

Describe the use and clinical characteristics of Saccharomyces boulardi ( Sb ) probiotic yeast among patients with Saccharomyces fungemia, according to a retrospective registry study of all Saccharomyces sp. (cdc.gov)
Determine the use of Sb probiotic yeast among patients with positive Saccharomyces culture findings in samples other than blood, according to a retrospective registry study of all Saccharomyces sp. (cdc.gov)

To broadly explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways, we constructed a quantitative, high-density genetic interaction map (the MITO-MAP) in Saccharomyces cerevisiae. (thebiogrid.org)

The genetic effects of PR-toxin (56299004) were studied in Saccharomyces-cerevisiae and Neurospora-crassa. (cdc.gov)

Previously, we overexpressed the genes in the GSH biosynthetic pathway in Saccharomyces cerevisiae and observed a concomitant increase in the yield of ethanol (Ask 2013). (researchsquare.com)
Em uma segunda etapa, os hidrolisados ácido e enzimático e a mistura destes foram testados como meio de fermentação para duas linhagens recombinantes de Saccharomyces cerevisiae YRH 396 e YRH 400 que foram recombinadas por integração cromossômica dos genes da xilose redutase (XYL1), xilitol desidrogenase (XYL2) de Pichia stipitis e xiluloquinase (XKS1) de S. cerevisiae conferindo capacidade de metabolização da xilose. (ufrgs.br)
Summary of commonly expressed genes in S. cerevisiae 288C exposed to AgNPs and Ag-ions. (docsbay.net)

It is demonstrated that l-arabinose can be used as sole carbon and energy source by the recombinant industrial strain S. cerevisiae DS61180 and that cofactor and energy metabolism demand attention if the combined conversion of hexose and pentose sugars is intended, for example in biorefineries using lignocellulosics. (semanticscholar.org)

Antineutrophil Cytoplasmic Antibodies and Anti-Saccharomyces cerevisiae Antibody: Clinical Tools or Clues for Research? (mcmaster.ca)

Especie del género SACCHAROMYCES, familia Saccharomycetaceae, orden Saccharomycetales, conocida como levadura del pan o de la cerveza. (bvsalud.org)

We review the mechanisms responsible for amino acid homeostasis in Saccharomyces cerevisiae and other fungi. (nih.gov)

This review aims to discuss mechanistic details of mRNA localization in S. cerevisia. (elsevier.com)

Intervention group will receive 1 oral capsule once a day for 3 months containing 7x10^10 CFU Lactobacillus plantarum 299v (DSM9843), 5x10^9 CFU Saccharomyces cerevisiae var. (who.int)

The characterization of the starter Saccharomyces cerevisiae needs to be performed also taking into account this ability even if evaluated together with the overall metabolic profile. (frontiersin.org)

cdc42 GTP-Binding Protein, Saccharomyces cerevisiae" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)

Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S. cerevisiae BY4709 (wild type) cultured on 3 different C-sources (glucose, glycerol, and ethanol) and sampled along 10 time points with 5 biological replicates. (chalmers.se)
Methods Here, S. cerevisiae response to long-term rapamycin exposure was investigated by identifying the physiological, transcriptomic and metabolic differences observed for yeast populations inoculated into low-dose rapamycin-containing environment. (cam.ac.uk)

We present a novel method that utilizes the expression and binding patterns of the neighboring nodes of each link in existing experimentally-based, literature-derived gene transcriptional regulatory networks and extend them in silico using TF-gene binding motifs and a compendium of large expression data from Saccharomyces cerevisiae . (biomedcentral.com)
Role of multifunctional autonomously replicating sequence binding factor 1 in the initiation of DNA replication and transcriptional control in Saccharomyces cerevisiae. (yeastgenome.org)

In Saccharomyces cerevisiae asymmetric sorting of mRNA to the bud has been reported for at least 24 mRNAs. (elsevier.com)

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc15, a protein kinase involves in cytokinesis. (cellimagelibrary.org)

Streptococcus mutans, Staphylococcus aureus, Candida albicans and Saccharomyces cerevisiae . (bvsalud.org)

During alcoholic fermentation, Saccharomyces cerevisiae can be responsible for the production of several sulfur compounds via the sulfate reduction pathway ( Swiegers and Pretorius, 2007 ), but the majority of H 2 S produced during winemaking occurs as a result of the biosynthesis of the sulfur containing amino acids, methionine, and cysteine, which occur in low concentrations in grape juice, through the sulfate reduction sequence (SRS). (frontiersin.org)

The CLU1 gene encodes a protein that may associate with the core complex of eukaryotic translation initiation factor 3 (eIF3) in Saccharomyces cerevisiae. (suny.edu)

100% Vegetarian capsule (cellulose, water), saccharomyces cerevisiae. (hilifevitamins.com)

NOD2 mutations and anti-Saccharomyces cerevisiae antibodies are risk factors for Crohn's disease in African Americans. (cdc.gov)

Saccharomyces cerevisiae contains two paralogs of TOR, Tor1 and Tor2, which exist in their respective multi-subunit complexes TORC1 and TORC2. (berkeley.edu)

Preparation stater done by culturing medium Saccharomyces cereviseae as developer on 10% yeast fermentation medium thus able to adapt and be ready to do the fermentation. (unri.ac.id)
Extracellular hPON1 enzyme was expressed with the pPICZ?A vector using a strong AOX promoter, and enzyme secretion in the fermentation medium was achieved by means of Saccharomyces cerevisiae alpha factor signal sequence. (tubitak.gov.tr)

These results, call into serious question the utility of the BXB1 serine integrase, and possibly serine integrases in general, as tools for synthetic biology in Saccharomyces cerevisiae. (concordia.ca)

Saccharomyces literally means sugar fungus. (giantmicrobes.com)

This study was conducted to determine if feeding a Saccharomyces cerevisiae fermentation product (SCFP) to calves would alter the acute phase response to a lipopolysaccharide (LPS) challenge. (quanterix.com)

S. cerevisiae can reproduce in anaerobic and aerobic conditions and accumulate ethanol at high concentrations, making it the preferred choice for starter cultures for beverage and food fermentations 20 . (nature.com)

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV. (genscript.com)

Anatomy21

Organisms14

Diseases1

Chemicals and Drugs117

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes88

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care3

Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. (1/41576)

Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. (2/41576)

The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. (3/41576)

Cooperative binding of heat shock factor to the yeast HSP82 promoter in vivo and in vitro. (4/41576)

Correlation between protein and mRNA abundance in yeast. (5/41576)

The Saccharomyces cerevisiae ETH1 gene, an inducible homolog of exonuclease III that provides resistance to DNA-damaging agents and limits spontaneous mutagenesis. (6/41576)

The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae. (7/41576)

A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. (8/41576)

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Fungal Proteins

Genes, Fungal

Gene Expression Regulation, Fungal

Saccharomyces

Molecular Sequence Data

Mutation

RNA, Fungal

Amino Acid Sequence

Base Sequence

DNA, Fungal

Chromosomes, Fungal

Genome, Fungal

Cloning, Molecular

Plasmids

Genetic Complementation Test

Sequence Homology, Amino Acid

Gene Deletion

Suppression, Genetic

Transcription, Genetic

Phenotype

Spores, Fungal

DNA-Binding Proteins

beta-Fructofuranosidase

Restriction Mapping

Transcription Factors

Genes, Suppressor

Haploidy

Mutagenesis

Vacuoles

Transformation, Genetic

Fermentation

Recombination, Genetic

Temperature

Diploidy

Cell Cycle Proteins

Kinetics

Mitochondria

Silent Information Regulator Proteins, Saccharomyces cerevisiae

Recombinant Fusion Proteins

Sequence Alignment

Schizosaccharomyces

Protein Binding

Glucose

Alleles

Membrane Proteins

Meiosis

Genes, Mating Type, Fungal

DNA Repair

Saccharomycetales

Nuclear Proteins

Kluyveromyces

CDC28 Protein Kinase, S cerevisiae

Spheroplasts

Escherichia coli

Adenosine Triphosphatases

DNA Primers

RNA, Messenger

Galactose

Recombinant Proteins

Cell Cycle

Repressor Proteins

Cell Wall

Ergosterol

Candida albicans

Killer Factors, Yeast

Binding Sites

Pheromones

Substrate Specificity

Carrier Proteins

Open Reading Frames

Promoter Regions, Genetic

Biological Transport

Genes

Sequence Deletion

Culture Media

Protein Structure, Tertiary

Xylose

Sirtuin 2