A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Proteins found in any species of fungus.
The functional hereditary units of FUNGI.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
A genus of ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Structures within the nucleus of fungal cells consisting of or containing DNA, which carry genetic information essential to the cell.
The complete gene complement contained in a set of chromosomes in a fungus.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Reproductive bodies produced by fungi.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A glycoside hydrolase found primarily in PLANTS and YEASTS. It has specificity for beta-D-fructofuranosides such as SUCROSE.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Genes that have a suppressor allele or suppressor mutation (SUPPRESSION, GENETIC) which cancels the effect of a previous mutation, enabling the wild-type phenotype to be maintained or partially restored. For example, amber suppressors cancel the effect of an AMBER NONSENSE MUTATION.
The chromosomal constitution of cells, in which each type of CHROMOSOME is represented once. Symbol: N.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
The rate dynamics in chemical or physical systems.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
A set of nuclear proteins in SACCHAROMYCES CEREVISIAE that are required for the transcriptional repression of the silent mating type loci. They mediate the formation of silenced CHROMATIN and repress both transcription and recombination at other loci as well. They are comprised of 4 non-homologous, interacting proteins, Sir1p, Sir2p, Sir3p, and Sir4p. Sir2p, an NAD-dependent HISTONE DEACETYLASE, is the founding member of the family of SIRTUINS.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.
Fungal genes that mostly encode TRANSCRIPTION FACTORS. In some FUNGI they also encode PHEROMONES and PHEROMONE RECEPTORS. The transcription factors control expression of specific proteins that give a cell its mating identity. Opposite mating type identities are required for mating.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.
A protein kinase encoded by the Saccharomyces cerevisiae CDC28 gene and required for progression from the G1 PHASE to the S PHASE in the CELL CYCLE.
Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Proteins prepared by recombinant DNA technology.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
A steroid of interest both because its biosynthesis in FUNGI is a target of ANTIFUNGAL AGENTS, notably AZOLES, and because when it is present in SKIN of animals, ULTRAVIOLET RAYS break a bond to result in ERGOCALCIFEROL.
A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).
Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Chemical substances, excreted by an organism into the environment, that elicit behavioral or physiological responses from other organisms of the same species. Perception of these chemical signals may be olfactory or by contact.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Transport proteins that carry specific substances in the blood or across cell membranes.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Deletion of sequences of nucleic acids from the genetic material of an individual.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A sirtuin family member found primarily in the CYTOPLASM. It is a multifunctional enzyme that contains a NAD-dependent deacetylase activity that is specific for HISTONES and a mono-ADP-ribosyltransferase activity.
Genes whose loss of function or gain of function MUTATION leads to the death of the carrier prior to maturity. They may be essential genes (GENES, ESSENTIAL) required for viability, or genes which cause a block of function of an essential gene at a time when the essential gene function is required for viability.
The process by which a DNA molecule is duplicated.
An enzyme that converts UDP glucosamine into chitin and UDP. EC
Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
A carboxypeptidase that catalyzes the release of a C-terminal amino acid with a broad specificity. It also plays a role in the LYSOSOMES by protecting BETA-GALACTOSIDASE and NEURAMINIDASE from degradation. It was formerly classified as EC and EC
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Any method used for determining the location of and relative distances between genes on a chromosome.
A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.
Fermented juice of fresh grapes or of other fruit or plant products used as a beverage.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.
Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC, EC, EC, and EC
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction.
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS from SACCHAROMYCES CEREVISIAE. It is involved in morphological events related to the cell cycle. This enzyme was formerly listed as EC
A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
A DNA-binding protein that mediates DNA REPAIR of double strand breaks, and HOMOLOGOUS RECOMBINATION.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.
A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.
A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.
An alkylating agent in cancer therapy that may also act as a mutagen by interfering with and causing damage to DNA.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
A systemic agricultural fungicide used for control of certain fungal diseases of stone fruit.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The sum of the weight of all the atoms in a molecule.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.
An enzyme that catalyzes the conversion of alpha,alpha-trehalose and water to D-glucose. EC
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A family of pheromone receptors that were initially discovered in SACCHAROMYCES CEREVISIAE as proteins necessary for fungal conjugation. Each mating factor receptor is expressed in HAPLOID CELLS of a single mating type.
Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.
An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman.
A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.
A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
An enzyme that catalyzes reversibly the formation of galactose 1-phosphate and ADP from ATP and D-galactose. Galactosamine can also act as the acceptor. A deficiency of this enzyme results in GALACTOSEMIA. EC
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Toxic compounds produced by FUNGI.
Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.
The asymmetrical segregation of genes during replication which leads to the production of non-reciprocal recombinant strands and the apparent conversion of one allele into another. Thus, e.g., the meiotic products of an Aa individual may be AAAa or aaaA instead of AAaa, i.e., the A allele has been converted into the a allele or vice versa.
Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Microbodies which occur in animal and plant cells and in certain fungi and protozoa. They contain peroxidase, catalase, and allied enzymes. (From Singleton and Sainsbury, Dictionary of Microbiology and Molecular Biology, 2nd ed)
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Cellular proteins and protein complexes that transport amino acids across biological membranes.
Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)
A class of enzymes that form a thioester bond to UBIQUITIN with the assistance of UBIQUITIN-ACTIVATING ENZYMES. They transfer ubiquitin to the LYSINE of a substrate protein with the assistance of UBIQUITIN-PROTEIN LIGASES.
Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.
The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Life or metabolic reactions occurring in an environment containing oxygen.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.
Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.
A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed)
The reciprocal exchange of segments at corresponding positions along pairs of homologous CHROMOSOMES by symmetrical breakage and crosswise rejoining forming cross-over sites (HOLLIDAY JUNCTIONS) that are resolved during CHROMOSOME SEGREGATION. Crossing-over typically occurs during MEIOSIS but it may also occur in the absence of meiosis, for example, with bacterial chromosomes, organelle chromosomes, or somatic cell nuclear chromosomes.
A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-
Those genes found in an organism which are necessary for its viability and normal function.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.
Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.

Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. (1/41576)

The product of the oskar gene directs posterior patterning in the Drosophila oocyte, where it must be deployed specifically at the posterior pole. Proper expression relies on the coordinated localization and translational control of the oskar mRNA. Translational repression prior to localization of the transcript is mediated, in part, by the Bruno protein, which binds to discrete sites in the 3' untranslated region of the oskar mRNA. To begin to understand how Bruno acts in translational repression, we performed a yeast two-hybrid screen to identify Bruno-interacting proteins. One interactor, described here, is the product of the apontic gene. Coimmunoprecipitation experiments lend biochemical support to the idea that Bruno and Apontic proteins physically interact in Drosophila. Genetic experiments using mutants defective in apontic and bruno reveal a functional interaction between these genes. Given this interaction, Apontic is likely to act together with Bruno in translational repression of oskar mRNA. Interestingly, Apontic, like Bruno, is an RNA-binding protein and specifically binds certain regions of the oskar mRNA 3' untranslated region.  (+info)

Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. (2/41576)

The vacuolar protein sorting (VPS) pathway of Saccharomyces cerevisiae mediates transport of vacuolar protein precursors from the late Golgi to the lysosome-like vacuole. Sorting of some vacuolar proteins occurs via a prevacuolar endosomal compartment and mutations in a subset of VPS genes (the class D VPS genes) interfere with the Golgi-to-endosome transport step. Several of the encoded proteins, including Pep12p/Vps6p (an endosomal target (t) SNARE) and Vps45p (a Sec1p homologue), bind each other directly [1]. Another of these proteins, Vac1p/Pep7p/Vps19p, associates with Pep12p and binds phosphatidylinositol 3-phosphate (PI(3)P), the product of the Vps34 phosphatidylinositol 3-kinase (PI 3-kinase) [1] [2]. Here, we demonstrate that Vac1p genetically and physically interacts with the activated, GTP-bound form of Vps21p, a Rab GTPase that functions in Golgi-to-endosome transport, and with Vps45p. These results implicate Vac1p as an effector of Vps21p and as a novel Sec1p-family-binding protein. We suggest that Vac1p functions as a multivalent adaptor protein that ensures the high fidelity of vesicle docking and fusion by integrating both phosphoinositide (Vps34p) and GTPase (Vps21p) signals, which are essential for Pep12p- and Vps45p-dependent targeting of Golgi-derived vesicles to the prevacuolar endosome.  (+info)

The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. (3/41576)

Polarized secretion requires proper targeting of secretory vesicles to specific sites on the plasma membrane. Here we report that the exocyst complex plays a key role in vesicle targeting. Sec15p, an exocyst component, can associate with secretory vesicles and interact specifically with the rab GTPase, Sec4p, in its GTP-bound form. A chain of protein-protein interactions leads from Sec4p and Sec15p on the vesicle, through various subunits of the exocyst, to Sec3p, which marks the sites of exocytosis on the plasma membrane. Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion.  (+info)

Cooperative binding of heat shock factor to the yeast HSP82 promoter in vivo and in vitro. (4/41576)

Previous work has shown that heat shock factor (HSF) plays a central role in remodeling the chromatin structure of the yeast HSP82 promoter via constitutive interactions with its high-affinity binding site, heat shock element 1 (HSE1). The HSF-HSE1 interaction is also critical for stimulating both basal (noninduced) and induced transcription. By contrast, the function of the adjacent, inducibly occupied HSE2 and -3 is unknown. In this study, we examined the consequences of mutations in HSE1, HSE2, and HSE3 on HSF binding and transactivation. We provide evidence that in vivo, HSF binds to these three sites cooperatively. This cooperativity is seen both before and after heat shock, is required for full inducibility, and can be recapitulated in vitro on both linear and supercoiled templates. Quantitative in vitro footprinting reveals that occupancy of HSE2 and -3 by Saccharomyces cerevisiae HSF (ScHSF) is enhanced approximately 100-fold through cooperative interactions with the HSF-HSE1 complex. HSE1 point mutants, whose basal transcription is virtually abolished, are functionally compensated by cooperative interactions with HSE2 and -3 following heat shock, resulting in robust inducibility. Using a competition binding assay, we show that the affinity of recombinant HSF for the full-length HSP82 promoter is reduced nearly an order of magnitude by a single-point mutation within HSE1, paralleling the effect of these mutations on noninduced transcript levels. We propose that the remodeled chromatin phenotype previously shown for HSE1 point mutants (and lost in HSE1 deletion mutants) stems from the retention of productive, cooperative interactions between HSF and its target binding sites.  (+info)

Correlation between protein and mRNA abundance in yeast. (5/41576)

We have determined the relationship between mRNA and protein expression levels for selected genes expressed in the yeast Saccharomyces cerevisiae growing at mid-log phase. The proteins contained in total yeast cell lysate were separated by high-resolution two-dimensional (2D) gel electrophoresis. Over 150 protein spots were excised and identified by capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein spots were quantified by metabolic labeling and scintillation counting. Corresponding mRNA levels were calculated from serial analysis of gene expression (SAGE) frequency tables (V. E. Velculescu, L. Zhang, W. Zhou, J. Vogelstein, M. A. Basrai, D. E. Bassett, Jr., P. Hieter, B. Vogelstein, and K. W. Kinzler, Cell 88:243-251, 1997). We found that the correlation between mRNA and protein levels was insufficient to predict protein expression levels from quantitative mRNA data. Indeed, for some genes, while the mRNA levels were of the same value the protein levels varied by more than 20-fold. Conversely, invariant steady-state levels of certain proteins were observed with respective mRNA transcript levels that varied by as much as 30-fold. Another interesting observation is that codon bias is not a predictor of either protein or mRNA levels. Our results clearly delineate the technical boundaries of current approaches for quantitative analysis of protein expression and reveal that simple deduction from mRNA transcript analysis is insufficient.  (+info)

The Saccharomyces cerevisiae ETH1 gene, an inducible homolog of exonuclease III that provides resistance to DNA-damaging agents and limits spontaneous mutagenesis. (6/41576)

The recently sequenced Saccharomyces cerevisiae genome was searched for a gene with homology to the gene encoding the major human AP endonuclease, a component of the highly conserved DNA base excision repair pathway. An open reading frame was found to encode a putative protein (34% identical to the Schizosaccharomyces pombe eth1(+) [open reading frame SPBC3D6.10] gene product) with a 347-residue segment homologous to the exonuclease III family of AP endonucleases. Synthesis of mRNA from ETH1 in wild-type cells was induced sixfold relative to that in untreated cells after exposure to the alkylating agent methyl methanesulfonate (MMS). To investigate the function of ETH1, deletions of the open reading frame were made in a wild-type strain and a strain deficient in the known yeast AP endonuclease encoded by APN1. eth1 strains were not more sensitive to killing by MMS, hydrogen peroxide, or phleomycin D1, whereas apn1 strains were approximately 3-fold more sensitive to MMS and approximately 10-fold more sensitive to hydrogen peroxide than was the wild type. Double-mutant strains (apn1 eth1) were approximately 15-fold more sensitive to MMS and approximately 2- to 3-fold more sensitive to hydrogen peroxide and phleomycin D1 than were apn1 strains. Elimination of ETH1 in apn1 strains also increased spontaneous mutation rates 9- or 31-fold compared to the wild type as determined by reversion to adenine or lysine prototrophy, respectively. Transformation of apn1 eth1 cells with an expression vector containing ETH1 reversed the hypersensitivity to MMS and limited the rate of spontaneous mutagenesis. Expression of ETH1 in a dut-1 xthA3 Escherichia coli strain demonstrated that the gene product functionally complements the missing AP endonuclease activity. Thus, in apn1 cells where the major AP endonuclease activity is missing, ETH1 offers an alternate capacity for repair of spontaneous or induced damage to DNA that is normally repaired by Apn1 protein.  (+info)

The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae. (7/41576)

Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5'-->3' Exo1 and the 3'-->5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) mutants defective in 3'-->5' proofreading exonuclease, mutants defective in the 5'-->3' exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes.  (+info)

A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. (8/41576)

The assembly pathway of spliceosomal snRNPs in yeast is poorly understood. We devised a screen to identify mutations blocking the assembly of newly synthesized U4 snRNA into a functional snRNP. Fifteen mutant strains failing either to accumulate the newly synthesized U4 snRNA or to assemble a U4/U6 particle were identified and categorized into 13 complementation groups. Thirteen previously identified splicing-defective prp mutants were also assayed for U4 snRNP assembly defects. Mutations in the U4/U6 snRNP components Prp3p, Prp4p, and Prp24p led to disassembly of the U4/U6 snRNP particle and degradation of the U6 snRNA, while prp17-1 and prp19-1 strains accumulated free U4 and U6 snRNA. A detailed analysis of a newly identified mutant, the sad1-1 mutant, is presented. In addition to having the snRNP assembly defect, the sad1-1 mutant is severely impaired in splicing at the restrictive temperature: the RP29 pre-mRNA strongly accumulates and splicing-dependent production of beta-galactosidase from reporter constructs is abolished, while extracts prepared from sad1-1 strains fail to splice pre-mRNA substrates in vitro. The sad1-1 mutant is the only splicing-defective mutant analyzed whose mutation preferentially affects assembly of newly synthesized U4 snRNA into the U4/U6 particle. SAD1 encodes a novel protein of 52 kDa which is essential for cell viability. Sad1p localizes to the nucleus and is not stably associated with any of the U snRNAs. Sad1p contains a putative zinc finger and is phylogenetically highly conserved, with homologues identified in human, Caenorhabditis elegans, Arabidospis, and Drosophila.  (+info)

All alcohols and spirits such as beer need yeast for the purpose of fermentation and leading breweries understand that getting top quality beer with saccharomyces cerevisiae yeast is the only way to happily placate parched throats of enthusiastic drinkers all around the globe.. All types of alcohols and also spirits like beer, wines, whiskey, rum, vodka, and so on have got diverse alcohol strengths. Different types of yeast as well can merely ferment as well as survive within a variety of alcohols, and are additionally limited by temperature distillersyeast. Thus alcoholic beverage manufacturing involving the production of vodka cannot use yeast suitable for lower alcohol potency yeasts such as wine yeast or even other forms of yeast such as saccharomyces cerevisiae, which is essentially used for brewing beer.. The saccharomyces cerevisiae yeast is actually belonging to the fungi family similar to its other cousins which ferment various other kinds of alcohols. This particular yeast is often ...
Saccharomyces Cerevisiae Yeast Cells Sem Scanning as a 8x6 Glass Mount from CMSP Photo Prints. Fast and safe delivery. Saccharomyces Cerevisiae Yeast Cells. these Microorganisms Fungi are Used to Raise Bread Dough the Yeasts Produce
Saccharomyces Cerevisiae Yeast Cells Sem Scanning as a A2 (42x59 cm) Fine Art Print from CMSP Photo Prints. Fast and safe delivery. Saccharomyces Cerevisiae Yeast Cells. these Microorganisms Fungi are Used to Raise Bread Dough the Yeasts Produce
TY - JOUR. T1 - Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles. AU - Athenstaedt, Karin. AU - Daum, Günther. PY - 2005. Y1 - 2005. M3 - Article. VL - 280. SP - 37301. EP - 37109. JO - The Journal of Biological Chemistry. JF - The Journal of Biological Chemistry. SN - 0021-9258. ER - ...
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SCU48699 U48699 414bp mRNA PLN 10-APR-1996 Saccharomyces cerevisiae ribosomal protein S30 homolog (RPS30A) mRNA, complete cds. RPS30A; Rps30ap. SCU48700 U48700 1200bp DNA PLN 10-APR-1996 Saccharomyces cerevisiae ribosomal protein S30 homolog (RPS30) gene, complete cds. RPS30A; yrpS30. SCU51431 U51431 2760bp DNA PLN 10-APR-1996 Saccharomyces cerevisiae PHD5 gene, complete cds. PHD5; Phd5p. YSCL8004 U53876 40340bp DNA PLN 10-APR-1996 Saccharomyces cerevisiae chromosome XII cosmid 8004. L8004.4; L8004.5; L8004.6; KIN2; Probable Serine/Threonine protein kinase (Swiss; Prot. accession number P13186); ; L8004.7; CHA4; Transcriptional activator of CHA1 (Swiss Prot.; accession number P43634). Contains a Cys(6) zinc finger.; ; L8004.9; L8004.2; L8004.10; L8004.11; SEN2; tRNA-splicing endonuclease beta-subunit; L8004.13; L8004.1. YSCL9233 U53877 23223bp DNA PLN 10-APR-1996 Saccharomyces cerevisiae chromosome XII cosmid 9233. L9233.6; L9233.7; L9233.8; L9233.5; YAP3; Aspartic proteinase 3 (Swiss Prot. ...
In the study, 300 male day-old, Ross 308 broiler chicks were used. Experiment groups were designed as follows: control; 0.1 % Saccharomyces cerevisiae; 0.2 % Saccharomyces cerevisiae; 0.4 % Saccharomyces cerevisiae. The experimental diets were chemically analyzed according to the methods of the Association of Official Analytical Chemists. Twelve groups were obtained, including three replicates for each experimental group. Each replicated group was comprised of 25 chicks, and thus 75 chicks were placed in each experimental group. After 42 days, broiler chickens were slaughtered. Tibiotarsi were weighed with a digital scale, and the lengths were measured with a digital caliper after the drying process. Cortical areas were measured with the ImageJ Image Processing and Analysis Program. A UTEST Model-7014 tension and compression machine and a Maxtest software were used to determine the bone strength of the tibiotarsus. The severity of the tibial dyschondroplasia lesion was evaluated as 0, +1, +2 and ...
The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry bakers yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1→3)-linked β-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1→3)-β-oligoglucosides, linked to the main chaing through (1→6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the β-D-configuration ...
The effect of yeast (Saccharomyces cerevisiae) on fattening performances of growing cattle is an article from MOJ Ecology & Environmental Sciences for MedCrave Group. The aim of this experiment was to evaluate the yeast on fattening performances of the growing cattle. The experiment was carried out with 179 imported 12-14 months old growing mixed breed bulls (Hereford, Angus, Brangus, and some other crossbreds) that were allocated to control and yeast group according to the breeds and body weight. Experimental diet was formulated with 19 % roughages (alfalfa and wheat straw) containing 13% crude protein. Yeast group was supplemented 40g d-1 live yeast containing 1.23×1011 CFU/g. The study lasted 62 days from May to July. Initial body weight were 393.91±4,43 for control and 395.56±4.45kg for yeast group. After test period, daily gain was similar (1465.85±26.76 vs. 1451.42±34.05g d-1, P|0.05) for the bull receiving the diet without yeast compared to the bulls receiving yeast. Similar results were
TY - JOUR. T1 - Electron transport chain of Saccharomyces cerevisiae mitochondria is inhibited by H2O2 at succinate-cytochrome c oxidoreductase level without lipid peroxidation involvement. AU - Cortés-Rojo, Christian. AU - Calderó;n-Cortés, Elizabeth. AU - Clemente-Guerrero, Mónica. AU - Manzo-Ávalos, Salvador. AU - Uribe, Salvador. AU - Boldogh, Istvan. AU - Saavedra-Molina, Alfredo. PY - 2007/11. Y1 - 2007/11. N2 - The deleterious effects of H2O2 on the electron transport chain of yeast mitochondria and on mitochondrial lipid peroxidation were evaluated. Exposure to H2O2 resulted in inhibition of the oxygen consumption in the uncoupled and phosphorylating states to 69% and 65%, respectively. The effect of H2O2 on the respiratory rate was associated with an inhibition of succinate-ubiquinone and succinate-DCIP oxidoreductase activities. Inhibitory effect of H2O2 on respiratory complexes was almost completely recovered by β-mercaptoethanol treatment. H2O2 treatment resulted in full ...
New Sequences ============= S82971 S82971 1775bp DNA PLN 10-FEB-1997 PEX13=PAS20 [Saccharomyces cerevisiae, Genomic, 1775 nt]. PEX13; Pex13p. SCRGA1 X90950 4305bp DNA PLN 07-FEB-1997 S.cerevisiae rga1 (dbm1) gene. DBM1; pheromone response; RGA1 gene; RGA1 (DBM1); Rga1p (Dbm1p). SCU17262 U17262 3051bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip1p (PIP1) gene, complete cds. PIP1; Pip1p. SCU17263 U17263 2251bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip2p (PIP2) gene, complete cds. PIP2; Pip2p. SCU17264 U17264 1842bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip3p (PIP3) gene, complete cds. PIP3; Pip3p. SCU85960 U85960 1720bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae RNA polymerase II-specific TBP associated factor Taf40p (TAF40) gene, complete cds. TAF40; RNA polymerase II specific TBP associated; factor. SCU86641 U86641 1657bp DNA PLN 08-FEB-1997 Saccharomyces cerevisiae Rim9p (RIM9) gene, complete cds. RIM9; Rim9p. =========== Updated Features/Annotations ============= YSCDYS1 ...
TY - JOUR. T1 - Magnesium as a stress-protectant for industrial strains of saccharomyces cerevisiae. AU - Walker, Graeme M.. PY - 1998. Y1 - 1998. N2 - During brewery fermentations, individual yeast cells may be confronted with a variety of environmental stresses that impair yeast growth and fermentative metabolism. An understanding of the stress physiology of industrial yeasts is therefore important in order to counteract deleterious effects of stress on fermentation and, ultimately, product quality. The present study describes the influence of magnesium ions in preventing cell death caused by temperature shock and ethanol toxicity in Saccharomyces cerevisiae yeast strains employed in brewing, distilling, and wine fermentations. Results obtained show that, by increasing the extracellular availability of magnesium ions, physiological protection may be conferred on temperature- and ethanol-stressed yeast cells with respect to culture viability and growth. This practical approach is envisaged to ...
TY - JOUR. T1 - Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae. AU - Higgins, David R.. AU - Prakash, Satya. AU - Reynolds, Paul. AU - Prakash, Louise. PY - 1983. Y1 - 1983. N2 - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.. AB - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or ...
TY - JOUR. T1 - Cooperative interactions between pairs of homologous chromatids during meiosis in Saccharomyces cerevisiae. AU - Mell, Joshua Chang. AU - Komachi, Kelly. AU - Hughes, Owen. AU - Burgess, Sean. PY - 2008/6. Y1 - 2008/6. N2 - We report a novel instance of negative interference during Saccharomyces cerevisiae meiosis, where Cremediated recombination between pairs of allelic loxP sites is more frequent than expected. We suggest that endogenous crossover recombination mediates cooperative pairing interactions between all four chromatids of a meiotic bivalent.. AB - We report a novel instance of negative interference during Saccharomyces cerevisiae meiosis, where Cremediated recombination between pairs of allelic loxP sites is more frequent than expected. We suggest that endogenous crossover recombination mediates cooperative pairing interactions between all four chromatids of a meiotic bivalent.. UR - http://www.scopus.com/inward/record.url?scp=49849083414&partnerID=8YFLogxK. UR - ...
TY - JOUR. T1 - The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase. AU - MACKELVIE, SARAH H. AU - ANDREWS, PAUL D.. AU - STARK, MICHAEL J. R. PY - 1995/7. Y1 - 1995/7. N2 - In higher eukaryotes, the activity and specificity of the type 1 protein serine-threonine phosphatase (PP1) catalytic subunit is thought to be controlled by its association with a number of regulatory or targeting subunits. Here we describe the characterization of a gene encoding one such potential polypeptide in the yeast Saccharomyces cerevisiae. The gene which we have isolated (termed SDS22) encodes a product with a high degree of sequence identity to the fission yeast sds22 protein, a known regulator of the mitotic function of PP1 in Schizosaccharomyces pombe. Using two different criteria, we have demonstrated that Sds22p and the catalytic subunit of PP1 (Glc7p) interact in yeast cells. We have also generated a temperature-sensitive allele of ...
In a previous attempt to identify as many as possible of the essential genes on Saccharomyces cerevisiae chromosome I, temperature-sensitive (Ts-) lethal mutations that had been induced by ethyl methane-sulfonate or nitrosoguanidine were analyzed. Thirty-two independently isolated mutations that mapped to chromosome I identified only three complementation groups, all of which had been known previously. In contrast, molecular analyses of segments of the chromosome have suggested the presence of numerous additional essential genes. In order to assess the degree to which problems of mutagen specificity had limited the set of genes detected using Ts- lethal mutations, we isolated a new set of such mutations after mutagenesis with UV or nitrogen mustard. Surprisingly, of 21 independently isolated mutations that mapped to chromosome I, 17 were again in the same three complementation groups as identified previously, and two of the remaining four mutations were apparently in a known gene involved in ...
ARAUJO, Roberta A.C. et al. Monitoring Saccharomyces cerevisiae populations by mtDNA restriction analysis and other molecular typing methods during spontaneous fermentation for production of the artisanal cachaça. Braz. J. Microbiol. [online]. 2007, vol.38, n.2, pp.217-223. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822007000200006.. An ecological study on Saccharomyces cerevisiae populations in spontaneous fermentation has been conducted in three vats of a cachaça distillery in Minas Gerais, Brazil. Ninety-seven yeast isolates were collected at the beginning, the middle and at the end of the production period, and were identified by standard methods. Differentiation between the indigenous S. cerevisiae strains isolated was performed by mitochondrial DNA (mtDNA) restriction analysis, RAPD-PCR, and PCR fingerprint using an intron splice primer. Analysis of the mtDNA restriction profiles revealed 12 different patterns, 11 corresponding to indigenous yeasts (I to XI) and one (XII) to a ...
TY - JOUR. T1 - Saccharomyces cerevisiae proteins involved in hybrid DNA formation in vitro. AU - Heyer, W. D.. AU - Johnson, A. W.. AU - Norris, D. N.. AU - Tishkoff, D.. AU - Kolodner, R. D.. PY - 1991. Y1 - 1991. N2 - RecA-like activities that can form hybrid DNA in vitro have been identified in a wide variety of organisms. We have previously described the strand exchange protein 1 (SEP1) from the yeast Saccharomyces cerevisiae that can form hybrid DNA in vitro. Purified as an Mr 132 000 polypeptide, recent molecular and immunological studies have now shown that the native form is an Mr 175 000 polypeptide containing strand exchange activity. The gene encoding SEP1 has been cloned and sequenced. The primary sequence failed to reveal any significant sequence homology to other sequences in data base searches. In vivo SEP1 was found to be essential for normal meiosis as cells containing a homozygous insertion mutation in the SEP1 gene failed to sporulate. In order to identify additional factors ...
Understanding how new biochemical pathways evolve in a sexually reproducing population is a complex and largely unanswered question. We have successfully evolved a novel biochemical pathway in yeast using a sex based population approach.. For over 30 years, wild type Saccharomyces has been widely reported to not grow on xylose at all, but we discovered that most strains can grow, albeit at almost undetectable rates. A mass mated starting population of Saccharomyces cerevisiae strains was evolved under selection on Xylose Minimal Media (XMM) with forced sexual mating every ~two months for 1463 days. This produced a population that could grow on xylose as a sole carbon source. Initial studies show the xylose growth trait is quantitative and presumably governed by many genes. To investigate the evolution of the xylose phenotype, a xylose utilising strain MBG11a was isolated. MBG11a was sequenced with PacBio RSII long read sequencing at the Ramaciotti Centre for Genomics. A high quality complete ...
TY - JOUR. T1 - Variation in indole-3-acetic acid production by wild Saccharomyces cerevisiae and S. paradoxus strains from diverse ecological sources and its effect on growth. AU - Liu, Yen Yu. AU - Chen, Hung Wei. AU - Chou, Jui Yu. PY - 2016/8/1. Y1 - 2016/8/1. N2 - Phytohormone indole-3-acetic acid (IAA) is the most common naturally occurring and most thoroughly studied plant growth regulator. Microbial synthesis of IAA has long been known. Microbial IAA biosynthesis has been proposed as possibly occurring through multiple pathways, as has been proven in plants. However, the biosynthetic pathways of IAA and the ecological roles of IAA in yeast have not been widely studied. In this study, we investigated the variation in IAA production and its effect on the growth of Saccharomyces cerevisiae and its closest relative Saccharomyces paradoxus yeasts from diverse ecological sources. We found that almost all Saccharomyces yeasts produced IAA when cultured in medium supplemented with the primary ...
TY - JOUR. T1 - A regulated MET3-GLC7 gene fusion provides evidence of a mitotic role for Saccharomyces cerevisiae protein phosphatase 1. AU - Black, S. AU - Andrews, P D. AU - Sneddon, A A. AU - Stark, M J. PY - 1995. Y1 - 1995. N2 - Saccharomyces cerevisiae possesses a single essential gene (GLC7) encoding protein phosphatase 1 (PP1). Elevated expression of this gene from the GAL1 promoter is highly detrimental to the cell, causing a growth defect and aberrant bud morphology, which leads to cells exhibiting long, extended buds. By comparison, expression of GLC7 from the weaker MET3 promoter was without significant effect on either growth or morphology. However, repression of GLC7 expression from the MET3 promoter in cells where the MET3-GLC7 fusion was the sole source of PP1 resulted in a mitotic delay. Such cultures showed a massive decrease in the rate of proliferation in conjunction with a significant increase in the proportion of large, budded cells. 46-diamidino-2-phenylindole ...
The yeast Saccharomyces cerevisiae is an important eukaryotic workhorse in traditional and modern biotechnology. At present, only a few S. cerevisiae strains have been extensively used as engineering hosts. Recently, an astonishing genotypic and phenotypic diversity of S. cerevisiae was disclosed in natural populations. We suppose that some natural strains can be recruited as superior host candidates in bioengineering. This study engineered a natural S. cerevisiae strain with advantages in inulin utilization to produce ethanol from inulin resources by consolidated bioprocess. Rational engineering strategies were employed, including secretive co-expression of heterologous exo- and endo-inulinases, repression of a protease, and switch between haploid and diploid strains. Results from co-expressing endo- and exo-inulinase genes showed that the extracellular inulinase activity increased 20 to 30-fold in engineered S. cerevisiae strains. Repression of the protease PEP4 influenced cell physiology in late
Saccharomyces cerevisiae Y12 - Organisms are classified by taxonomy into specified groups such as the multicellular animals, plants, and fungi; or unicellular microorganisms such as a protists, bacteria, and archaea.
Saccharomyces cerevisiae is a species of budding yeast. It is perhaps the most useful yeast owing to its use since ancient times in baking and brewing. It is believed that it was originally isolated from the skins of grapes (one can see the yeast as a component of the thin white film on the skins of some dark-colored fruits such as plums; it exists among the waxes of the cuticle). It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology, much like Escherichia coli as the model prokaryote. It is the microorganism behind the most common type of fermentation. Saccharomyces cerevisiae cells are round to ovoid, 5-10 micrometres in diameter. It reproduces by a division process known as budding. It is useful in studying the cell cycle because it is easy to culture, but, as a eukaryote, it shares the complex internal cell structure of plants and animals. S. cerevisiae was the first eukaryotic genome that was completely sequenced. The yeast genome database [1] is ...
Pulsed electric field (PEF) treatment can be used for non-thermal inactivation of microorganisms. The aim of this paper is to investigate PEF treatment of yeast, Saccharomyces cerevisiae, using three different field waveforms: square; non-oscillating exponential and oscillating exponential. The PEF system used in this paper consists of a pulsed power supply and a parallel-plane metallic electrodes treatment cell located in an air-pressurised chamber. PEF treatment of the yeast was conducted using electric field impulses with magnitudes of 67 kV/cm and 80 kV/cm. The efficacy of the PEF treatment for inactivation of the yeast cells was assessed by comparison of the PEF-treated and untreated yeast populations. Results showed that 3-log10 reduction in the yeast population can be achieved with 100 impulses using all tested waveforms. Amongst all three tested waveforms non-oscillating exponential impulses demonstrated improved PEF performance. The effect of duration of treatment and peak magnitude ...
I reveal that Saccharomyces cerevisiae Rtt109p promotes genome stability and resistance to DNA-damaging agents, and that it does this by functionally cooperating with the histone chaperone Asf1p to maintain normal chromatin structure. Furthermore, I show that, as for Asf1p, Rtt109p is required for histone H3 acetylation on lysine 56 (K56) in vivo. Moreover I show that Rtt109p directly catalyzes this modification in vitro in a manner that is stimulated by Asf1p. These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical fro cell survival in the presence of DNA damage during S phase. In the second part of this thesis, I reveal that cells deleted for Saccharomyces cerevisiae ESC2 exhibit synthetic sickness when combined with deletions of many genes involved in maintaining genomic stability. Moreover, I show that esc2Δ mutant cells exhibit increased recombination frequency and increased relocalisation of recombination repair protein ...
AbstractProtein-metabolite interactions are of crucial importance for all cellular processes but remain understudied. Here, we applied a biochemical approach named PROMIS, to address the complexity of the protein-small molecule interactome in the model yeast Saccharomyces cerevisiae. By doing so, we provide a unique dataset, which can be queried for interactions between 74 small molecules and 3982 proteins using a user-friendly interface available at https://promis.mpimp-golm.mpg.de/yeastpmi/. By interpolating PROMIS with the list of predicted protein-metabolite interactions, we provided experimental validation for 225 binding events. Remarkably, of the 74 small molecules co-eluting with proteins, 36 were proteogenic dipeptides. Targeted analysis of a representative dipeptide, Ser-Leu, revealed numerous protein interactors comprising chaperones, proteasomal subunits, and metabolic enzymes. We could further demonstrate that Ser-Leu binding increases activity of a glycolytic enzyme ...
Purchase Recombinant Saccharomyces cerevisiae Protein RTA1(RTA1). It is produced in in vitro E.coli expression system. High purity. Good price.
TY - JOUR. T1 - Investigation of steroid receptor function in the budding yeast Saccharomyces cerevisiae. AU - McEwan, I J PY - 1999. Y1 - 1999. N2 - Steroid hormones are small lipophilic molecules that control a wide range of responses in both the developing and adult organism. The actions of these molecules are mediated by soluble receptor proteins that function as hormone-activated transcription factors. The first steroid receptors were expressed in the yeast Saccharomyces cerevisae over 10 years ago, and to date virtually all the classical steroid receptors, together with a number of non-steroid members of the nuclear receptor superfamily, have been expressed in yeast. The ability to reconstitute steroid receptor signalling in yeast cells by co-expression of the receptor protein and a reporter gene driven by the appropriate hormone response element has presented researchers with a powerful model system for investigating receptor action. Tn this review, the use of yeast-based steroid receptor ...
The Saccharomyces cerevisiae SNF2 gene affects the expression of many diversely regulated genes and has been implicated in transcriptional activation. We report here the cloning and characterization of STH1, a gene that is homologous to SNF2. STH1 is essential for mitotic growth and is functionally distinct from SNF2. A bifunctional STH1-beta-galactosidase protein is located in the nucleus. The predicted 155,914-Da STH1 protein is 72% identical to SNF2 over 661 amino acids and 46% identical over another stretch of 66 amino acids. Both STH1 and SNF2 contain a putative nucleoside triphosphate-binding site and sequences resembling the consensus helicase motifs. The large region of homology shared by STH1 and SNF2 is conserved among other eukaryotic proteins, and STH1 and SNF2 appear to define a novel family of proteins related to helicases. ...
The Sec18 protein (Sec18p) of the yeast Saccharomyces cerevisiae has been identified as a component involved in the vesicular transport of proteins through the secretory and endocytotic pathways. Sec18p is a homologue of the mammalian protein NSF which has been shown, using a number of in vitro transport assay systems and affinity purification procedures, to interact with other proteins in a multisubunit protein complex. This work represents two approaches taken with the aim of identifying proteins that interact with Sec18p in the yeast Saccharomyces cerevisiae. Isolation of protein complexes was first attempted by affinity purification of a tagged version of Sec18p. The protein was C-terminally tagged with a protein A moiety from Staphylococcus aureus containing IgG binding domains. It was hoped that the affinity of protein A for IgG Sepharose could be used to isolate protein complexes that formed in vivo with the Sec18p. Although the fusion construct was shown to be active in vivo, specific ...
TY - CHAP. T1 - Delta integration CRISPR-Cas (Di-CRISPR) in saccharomyces cerevisiae. AU - Shi, Shuobo. AU - Liang, Youyun. AU - Ang, Ee Lui. AU - Zhao, Huimin. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Despite the advances made in genetic engineering of Saccharomyces cerevisiae, the multicopy genomic integration of large biochemical pathways remains a challenge. Here, we developed a Di-CRISPR (delta integration CRISPR-Cas) platform based on cleavage of the delta sites by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated systems (Cas) to enable unprecedented high-efficiency, multicopy, markerless integrations of large biochemical pathways into the S. cerevisiae genome. Detailed protocols are provided on the entire workflow which includes pDi-CRISPR plasmid and donor DNA construction, Di-CRISPR-mediated integration and analysis of integration efficiencies and copy numbers through flow cytometry and quantitative polymerase chain reaction (qPCR).. AB - Despite the ...
A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly use …
Yeast cells. Coloured Scanning Electron Micrograph (SEM) of yeast cells, Saccharomyces cerevisiae. This fungus, also known as Bakers or Brewers yeast, consists of single vegetative cells. Some cells can be seen dividing by budding off new cells. Saccharomyces cerevisiae ferments sugar, producing alcohol and carbon dioxide in the process. It has long been used in brewing beer, the production of wine and in baking leavened bread (carbon dioxide causes the dough to rise). Medically, dried Bakers yeast is used as a rich source of vitamin B1, riboflavin and nicotinic acid. Magnification: x125 at 6x7cm size. x200 at 4x5 - Stock Image B250/0646
Anhydrobiosis is the state of life when cells get into waterless conditions and gradually cease their metabolism. In this study, we determined the sequence of events in Saccharomyces cerevisiae energy metabolism during processes of dehydration and rehydration. The intensities of respiration and acidification of the medium, the amounts of Phenyldicarbaundecaborane (PCB-) bound to yeast membranes, and the capabilities of cells to accumulate K+ were assayed using electrochemical monitoring system, and intracellular content of ATP was measured using bioluminescence assay. Mesophilic, semi-resistant to desiccation S. cerevisiae strain 14 and thermotolerant, very resistant to desiccation S. cerevisiae strain 77 cells were compared. After 22 h of drying it was possible to restore the respiration activity of very resistant to desiccation strain 77 cells, especially when glucose was available. PCB- binding also indicated considerably higher metabolic activity of dehydrated S. cerevisiae strain 77 cells.
TY - JOUR. T1 - The effect of phosphate accumulation on metal ion homeostasis in Saccharomyces cerevisiae. AU - Rosenfeld, Leah. AU - Reddi, Amit R.. AU - Leung, Edison. AU - Aranda, Kimberly. AU - Jensen, Laran T.. AU - Culotta, Valeria C.. PY - 2010/9/1. Y1 - 2010/9/1. N2 - Much of what is currently understood about the cell biology of metals involves their interactions with proteins. By comparison, little is known about interactions of metals with intracellular inorganic compounds such as phosphate. Here we examined the role of phosphate in metal metabolism in vivo by genetically perturbing the phosphate content of Saccharomyces cerevisiae cells. Yeast pho80 mutants cannot sense phosphate and have lost control of phosphate uptake, storage, and metabolism. We report here that pho80 mutants specifically elevate cytosolic and nonvacuolar levels of phosphate and this in turn causes a wide range of metal homeostasis defects. Intracellular levels of the hard-metal cations sodium and calcium ...
Saccharomyces cerevisiae sudah sejak lama digunakan sebagai starter fermentasi pembuatan roti dan minuman beralkohol. Dalam buku ini, Saccharomyces crervisiae dimanfaatkan sebagai agensia modifikasi dalam pengolahan pangan, kemampuan S. cerevisiae dalam merombak komponen pangan, produk metabolit yang dihasilkan oleh S. cerevisiae, modifikasi terhadap perubahan sifat beberapa produk pangan oleh S. cerevisiae seperti tapioka, tempe, dan modifikasi fermentasi kakao. Pengertian dasar mengenai khamir perlu dipahami oleh mahasiswa yang khususnya mempelajari mikrobiologi pangan, mikrobiologi industri dan teknologi pangan. S.cerevisiae adalah khamir ...
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(11)2-Cys6 binuclear cluster domin and a putative alpha2-SCB-alpha2 binding site ...
Ghanegolmohammadia F, Yoshida M, Ohnuki S, Sukegawa Y, Okada H, Obara K, Kihara A, Suzuki K, Kojima T, Yachie N, Hirata D & Ohya Y Systematic analysis of Ca2+ homoeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles Molecular Biology of the Cell ...
Wine yeast (Saccharomyces cerevisiae D8) and non-Saccharomyces wine yeasts (Hanseniaspora uvarum S6 and Issatchenkia orientalis KMBL5774) were studied using air-blast drying instead of the conventional drying methods (such as freeze and spray drying). Skim milk-a widely used protective agent-was used and in all strains, the highest viabilities following air-blast drying were obtained using 10% skim milk. Four excipients (wheat flour, nuruk, artichoke powder, and lactomil) were evaluated as protective agents for yeast strains during air-blast drying. Our results showed that 7 g lactomil was the best excipient in terms of drying time, powder form, and the survival rate of the yeast in the final product. Finally, 7 types of sugars were investigated to improve the survival rate of air-blast dried yeast cells: 10% trehalose, 10% sucrose, and 10% glucose had the highest survival rate of 97.54, 92.59, and 79.49% for S. cerevisiae D8, H. uvarum S6, and I. orientalis KMBL5774, respectively. After 3 months of
Saccharomyces cerevisiae ATCC ® 201390D-5™ Designation: Genomic DNA from Saccharomyces cerevisiae Strain BY4743 (ATCC ® 201390™) Application:
Saccharomyces cerevisiae ATCC ® 201389D-5™ Designation: Genomic DNA from Saccharomyces cerevisiae Strain BY4742 (ATCC ® 201389™) Application:
Species, Publications, Genomes and Genes, Scientific Experts, Locale about Experts and Doctors on saccharomyces cerevisiae proteins in Al Ain, Abu Dhabi, United Arab Emirates
TY - JOUR. T1 - Automated Yeast Transformation Protocol to Engineer Saccharomyces cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames That Express Proteins Binding to Xylose Isomerase Identified Using a Robotic Two-Hybrid Screen. AU - Hughes, Stephen R.. AU - Rich, Joseph O.. AU - Bischoff, Kenneth M.. AU - Hector, Ronald E.. AU - Qureshi, Nasib. AU - Saha, Badal C.. AU - Dien, Bruce S.. AU - Liu, Siqing. AU - Jackson, John S.. AU - Sterner, David E.. AU - Butt, Tauseef R.. AU - LaBaer, Joshua. AU - Cotta, Michael A.. PY - 2009/8. Y1 - 2009/8. N2 - Commercialization of fuel ethanol production from lignocellulosic biomass has focused on engineering the glucose-fermenting industrial yeast Saccharomyces cerevisiae to use pentose sugars. Because S. cerevisiae naturally metabolizes xylulose, one approach involves introducing xylose isomerase (XI), which catalyzes conversion of xylose to xylulose. In this study, an automated two-hybrid interaction protocol was used to find ...
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TY - JOUR. T1 - Saccharomyces cerevisiae homologs of mammalian B and B subunits of protein phosphatase 2A direct the enzyme to distinct cellular functions. AU - Zhao, Yu. AU - Boguslawski, George. AU - Zitomer, Richard S.. AU - DePaoli-Roach, Anna A.. N1 - Copyright: Copyright 2007 Elsevier B.V., All rights reserved.. PY - 1997/3/28. Y1 - 1997/3/28. N2 - Protein phosphatase 2A (PP2A) is a major cellular serine/threonine protein phosphatase, present in the cell in a variety of heterotrimeric forms that differ in their associated regulatory B-subunit. Cloning of the mammalian B subunit has allowed the identification of a highly homologous Saccharomyces cerevisiae gene, RTS1. Disruption of the gene results in a temperature-sensitive growth defect that can be suppressed by expression of rabbit Bα or Bγ isoforms. The Bα subunit is much more effective in restoring normal growth at 37 °C than Bγ. Immunoprecipitated Rts1p was found associated with type 2A-specific protein phosphatase activity ...
Background and objectivePineapple peels contain significant quantities of carbohydrates, which can be used as cheap raw materials for production of commercially important products through fermentation. The aim of this study was to use this feed stock for the cultivation of Saccharomyces cerevisiae NCDC 364 and its use as single cell protein.Material and methodsThe single cell protein was produced using discarded pineapple peels and Saccharomyces cerevisiae NCDC 364. Optimization of bioprocess variables (temperature, pH, incubation period, carbon source and nitrogen source) affecting single cell protein production was carried out using classical one factor at a time approach. The harvested cells from optimized media were screened for amino acid content using high-performance thin-layer chromatography.Results andconclusionThe Saccharomyces cerevisiae NCDC 364 produced maximum single cell protein in pineapple peel based media, compared to non-optimized media. The one factor at a time approach showed
Base Sequence, DNA Polymerase II/chemistry, DNA Polymerase III/*chemistry/metabolism, DNA Replication, Molecular Sequence Data, Saccharomyces cerevisiae/enzymology/*genetics, Saccharomyces cerevisiae Proteins/*chemistry ...
This study evaluated the chemical and volatile composition of lychee wines fermented with four commercial yeast strains of Saccharomyces cerevisiae: EC-1118, R2, 71B and MERIT.ferm. Yeast cell population, pH, malic acid, ammonia and some amino acids had significant differences between strain 71B and other strains. There were strain variations with regard to degradation and retention of some juice volatiles as well as formation of new volatile compounds such as most esters and certain terpene compounds. Ethyl octanoate had the highest odour activity value (OAV of 500-1100), followed by ethyl hexanoate (about 50-85), among the common odorants in all lychee wines. Ethyl octanoate reached its highest OAV (1077) in the lychee wine fermented with strain EC1118, whereas ethyl hexanoate had the highest OAV (85) in the wine fermented with strain MERIT.ferm. Cis-rose oxide, the character-impact volatile in lychee juice with highest OAV (161), was dramatically reduced to trace levels after fermentation. © ...
Saccharomyces cerevisiae is the main microorganism responsible for the fermentation of wine. Nevertheless, in the last years wineries are facing new challenges due to current market demands and climate change effects on the wine quality. New yeast starters formed by non-conventional Saccharomyces species (such as S. uvarum or S. kudriavzevii) or their hybrids (S. cerevisiae x S. uvarum and S. cerevisiae x S. kudriavzevii) can contribute to solve some of these challenges. They exhibit good fermentative capabilities at low temperatures, producing wines with lower alcohol and higher glycerol amounts. However S. cerevisiae can competitively displace other yeast species from wine fermentations, therefore the use of these new starters requires an analysis of their behaviour during competition with S. cerevisiae during wine fermentation. In the present study we analyzed the survival capacity of non-cerevisiae strains in competition with S. cerevisiae during fermentation of synthetic wine must at different
It is essential when studying the circadian rhythm in cells to be able to effectively stop them in time. In this experiment, we tested what would be the most successful killing agent on Saccharomyces cerevisiae. Six different agents were tested at different concentrations and amounts. After the S. cerevisiae was added to the test tube containing the agent, it was streaked on a plate after 5 and 10 minutes. The plates were incubated and then checked for growth. Ethanol was the most efficient killing agent. After an effective killing agent is determined, it can be used in further experiments measuring Gapdehydrogenase activity using a colorimetric assay to examine the circadian rhythm in Saccharomyces cerevisiae. Gapdehydrogenase results will also be presented.
Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in ...
RODERIC (Repositori dObjectes Digitals per a lEnsenyament la Recerca i la Cultura) es el repositorio institucional de la Universitat de València. Se concibe como una ventanilla única para el acceso y la difusión de la producción digital de la Universitat. RODERIC responde al compromiso de la Universitat con el movimiento de acceso abierto al conocimiento adquirido con su adhesión a la Declaración de Berlín (30 Septiembre de 2008).
Biotechnology for Biofuels. RESEARCH Open Access. Systematic and evolutionary engineering of a xylose isomerase-based pathway in Saccharomyces cerevisiae for efficient conversion yields. Sun-Mi Lee1,2, Taylor Jellison1 and Hal S Alper1,3*. Abstract. Background: Efficient xylose fermentation by yeast would improve the economical and sustainable nature of biofuels production from lignocellulosic biomass. However, the efficiency of xylose fermentation by the yeast Saccharomyces cerevisiae is suboptimal, especially in conversion yield, despite decades of research. Here, we present an improved performance of S. cerevisiae in xylose fermentation through systematic and evolutionary engineering approaches. Results: The engineering of S. cerevisiae harboring xylose isomerase-based pathway significantly improved the xylose fermentation performance without the need for intensive downstream pathway engineering. This strain contained two integrated copies of a mutant xylose isomerase, gre3 and pho13 deletion ...
TY - JOUR. T1 - Nuclear and nucleolar localization of Saccharomyces cerevisiae ribosomal proteins S22 and S25. AU - Timmers, A.C.J.. AU - Stuger, R.. AU - Schaap, P.J.. AU - van t Riet, J.. AU - Raue, H.A.. PY - 1999. Y1 - 1999. U2 - 10.1016/s0014-5793(99)00669-9. DO - 10.1016/s0014-5793(99)00669-9. M3 - Article. VL - 452. SP - 335. EP - 340. JO - FEBS Letters. JF - FEBS Letters. SN - 0014-5793. ER - ...
TY - JOUR. T1 - A dependent pathway of gene functions leading to chromosome segregation in saccharomyces cerevisiae. AU - Wood, John S.. AU - Hartwell, Leland H.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 1982/9/1. Y1 - 1982/9/1. N2 - Methyl-benzimidazole-2-ylcarbamate (MBC) inhibits the mitotic cell cycle of Saccharomyces cerevisiae at a stage subsequent to DNA synthesis and before the completion of nuclear division (Quinlan, R. A., C. I. Pogson, and K. Gull, 1980, J. Cell 5ci., 46: 341-352). The step in the cell cycle that is sensitive to M8C inhibition was ordered in reciprocal shift experiments with respect to the steps catalyzed by cdc gene products. Execution of the CDC7 step is required for the initiation of DNA synthesis and for completion of the MBC-sensitive step. Results obtained with mutants (cdc2, 6, 8, 9, and 21) defective in DNA replication and with an inhibitor of DNA replication (hydroxyurea) suggest that some DNA replication is required for ...
TY - JOUR. T1 - Metabolomics approach to reduce the Crabtree effect in continuous culture of Saccharomyces cerevisiae. AU - Imura, Makoto. AU - Iwakiri, Ryo. AU - Bamba, Takeshi. AU - Fukusaki, Eiichiro. PY - 2018/8/1. Y1 - 2018/8/1. N2 - The budding yeast Saccharomyces cerevisiae is an important microorganism for fermentation and the food industry. However, during production, S. cerevisiae commonly uses the ethanol fermentation pathway for glucose utilization if excess sugar is present, even in the presence of sufficient oxygen levels. This aerobic ethanol fermentation, referred to as the Crabtree effect, is one of the most significant reasons for low cell yield. To weaken the Crabtree effect in fed-batch and continuous culture, sugar flow should be limited. In addition, in continuous culture, the dilution rate must be reduced to avoid washing out cells. However, under such conditions, production speed might be sacrificed. It is difficult to solve this problem with the tradeoff between cell ...
Industrial biotechnology aims to develop robust microbial cell factories, such as Saccharomyces cerevisiae, to produce an array of added value chemicals presently dominated by petrochemical processes. Xylose is the second most abundant monosaccharide after glucose and the most prevalent pentose sugar found in lignocelluloses. Significant research efforts have focused on the metabolic engineering of S similar to cerevisiae for fast and efficient xylose utilization. This study aims to metabolically engineer S similar to cerevisiae, such that it can consume xylose as the exclusive substrate while maximizing carbon flux to biomass production. Such a platform may then be enhanced with complementary metabolic engineering strategies that couple biomass production with high value-added chemical. Saccharomyces cerevisiae, expressing xylose reductase, xylitol dehydrogenase and xylulose kinase, from the native xylose-metabolizing yeast Pichia stipitis, was constructed, followed by a directed evolution strategy to
TY - JOUR. T1 - L-carnosine affects the growth of Saccharomyces cerevisiae in a metabolism-dependent manner. AU - Cartwright, Stephanie P.. AU - Bill, Roslyn M.. AU - Hipkiss, Alan R.. N1 - © Cartwright et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.. PY - 2012/9/12. Y1 - 2012/9/12. N2 - The dipeptide L-carnosine (β-alanyl-L-histidine) has been described as enigmatic: it inhibits growth of cancer cells but delays senescence in cultured human fibroblasts and extends the lifespan of male fruit flies. In an attempt to understand these observations, the effects of L-carnosine on the model eukaryote, Saccharomyces cerevisiae, were examined on account of its unique metabolic properties; S. cerevisiae can respire aerobically, but like some tumor cells, it can also exhibit a metabolism in which aerobic ...
Abstract: The objective of this study was to select three strains of probiotic Saccharomyces cerevisiae and to evaluate the effect of S. cerevisiae and rumen bacteria isolate (MR4) supplementation and their combination on rumen fermentability and rumen microbial population. Experiment 1 was designed in a 4 x 5 factorial randomized block design with 3 replications. The first factor was S. cerevisiae strain consisted of control treatment (without S. cerevisiae supplementation), NBRC 10217, NRRL Y 567 and NRRL 12618, and the second factor was incubation time consisted of 0, 1, 2, 3, and 4 h. Ration was basal ration for feedlot with forage to concentrate ratio (F:C)= 60:40. Dosage of each treatment with S. cerevisiae was 5 x 1010 cfu/kg ration. Experiment 2 was designed in randomized block design with 4 treatments: P0= basal ration of feedlot; P1= P0 + S. cerevisiae; P2= P0 + MR4 isolate (5 x 107 cfu/kg ration); P3= P0 + S. cerevisiae and MR4 isolate. The result of experiment 1 showed that ...
Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is not yet completely understood. The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up on these studies, many homologs have been found in higher eukaryotes. Yeast remains a highly relevant model organism for studying autophagy, with a wide range of established methods to elucidate the molecular details of the autophagy pathway. In this review, we provide an overview of methods to study both selective and bulk autophagy, including intermediate steps in the yeast Saccharomyces cerevisiae. We compare different assays, discuss their advantages and
TY - JOUR. T1 - A dual approach for improving homogeneity of a human-type N-glycan structure in Saccharomyces cerevisiae. AU - Piirainen, Mari. AU - Boer, Harry. AU - de Ruijter, Jorg C.. AU - Frey, Alexander D.. PY - 2016. Y1 - 2016. N2 - N-glycosylation is an important feature of therapeutic and other industrially relevant proteins, and engineering of the N-glycosylation pathway provides opportunities for developing alternative, non-mammalian glycoprotein expression systems. Among yeasts, Saccharomyces cerevisiae is the most established host organism used in therapeutic protein production and therefore an interesting host for glycoengineering. In this work, we present further improvements in the humanization of the N-glycans in a recently developed S. cerevisiae strain. In this strain, a tailored trimannosyl lipid-linked oligosaccharide is formed and transferred to the protein, followed by complex-type glycan formation by Golgi apparatus-targeted human N-acetylglucosamine transferases. We ...
The yeast assimilatory sulfate reductase is a complex enzyme that is responsible for conversion of sulfite into sulfide. To obtain information on the nature of this enzyme, we isolated and sequenced the MET10 gene of Saccharomyces cerevisiae and a divergent MET10 allele from Saccharomyces carlsbergensis. The polypeptides deduced from the identically sized open reading frames (1,035 amino acids) of both MET10 genes have molecular masses of around 115 kDa and are 88% identical to each other. The transcript of S. cerevisiae MET10 has a size comparable to that of the open reading frame and is transcriptionally repressed by methionine in a way similar to that seen for other MET genes of S. cerevisiae. Distinct homology was found between the putative MET10-encoded polypeptide and flavin-interacting parts of the sulfite reductase flavoprotein subunit (encoded by cysJ) from Escherichia coli and several other flavoproteins. A significant N-terminal homology to pyruvate flavodoxin oxidoreductase (encoded ...
TY - JOUR. T1 - Characterization of a staurosporine- and temperature-sensitive mutant, stt1, of Saccharomyces cerevisiae. T2 - STT1 is allelic to PKC1. AU - Yoshida, Satoshi. AU - Ikeda, Eri. AU - Uno, Isao. AU - Mitsuzawa, Hiroshi. PY - 1992/2/1. Y1 - 1992/2/1. N2 - Staurosporine is an antibiotic that specifically inhibits protein kinase C. Fourteen staurosporine- and temperature-sensitive (stt) mutants of Saccharomyces cerevisiae were isolated and characterized. These mutants were divided into ten complementation groups, and characterized for their cross-sensitivity to K-252a, neomycin, or CaCl2, The STT1 gene was cloned and sequenced. The nucleotide sequence of the STT1 gene revealed that STT1 is the same gene as PKC1. The STT1 gene conferred resistance to staurosporine on wild-type cells, when present on a high copy number plasmid. STT1/stt1::HIS3 diploid cells were more sensitive to staurosporine than STT1/STT1 diploid cells. Analysis of temperature-sensitive stt1 mutants showed that the ...
Saccharomyces cerevisiae strains expressing D-xylose isomerase (XI) produce some of the highest reported ethanol yields from D-xylose. Unfortunately, most bacterial XIs that have been expressed in S. cerevisiae are either not functional, require additional strain modification, or have low affinity for D-xylose. This study analyzed several XIs from rumen and intestinal microorganisms to identify enzymes with improved properties for engineering S. cerevisiae for D-xylose fermentation. Four XIs originating from rumen and intestinal bacteria were isolated and expressed in a S. cerevisiae CEN.PK2-1C parental strain primed for D-xylose metabolism by over expression of its native D-xylulokinase. Three of the XIs were functional in S. cerevisiae, based on the strains ability to grow in D-xylose medium. The most promising strain, expressing the XI mined from Prevotella ruminicola TC2-24, was further adapted for aerobic and fermentative growth by serial transfers of D-xylose cultures under aerobic, and followed
TY - JOUR. T1 - Multiparameter analysis of apoptosis in puromycin-treated Saccharomyces cerevisiae. AU - Citterio, Barbara. AU - Albertini, Maria Cristina. AU - Ghibelli, Lina. AU - Falcieri, Elisabetta. AU - Battistelli, Michela. AU - Canonico, Barbara. AU - Rocchi, Marco B. L.. AU - Teodori, Laura. AU - Ciani, Maurizio. AU - Piatti, Elena. PY - 2015/8/25. Y1 - 2015/8/25. N2 - In Saccharomyces cerevisiae, a typical apoptotic phenotype is induced by some stress factors such as sugars, acetic acid, hydrogen peroxide, aspirin and age. Nevertheless, no data have been reported for apoptosis induced by puromycin, a damaging agent known to induce apoptosis in mammalian cells. We treated S. cerevisiae with puromycin to induce apoptosis and evaluated the percentage of dead cells by using Hoechst 33342 staining, transmission electron microscopy (TEM) and Annexin V flow cytometry (FC) analysis. Hoechst 33342 fluorescence images were processed to acquire parameters to use for multiparameter analysis [and ...
A number of proteins have been isolated from human cells on the basis of their ability to support DNA replication in vitro of the simian virus 40 (SV40) origin of DNA replication. One such protein, replication factor C (RFC), functions with the proliferating cell nuclear antigen (PCNA), replication protein A (RPA), and DNA polymerase delta to synthesize the leading strand at a replication fork. To determine whether these proteins perform similar roles during replication of DNA from origins in cellular chromosomes, we have begun to characterize functionally homologous proteins from the yeast Saccharomyces cerevisiae. RFC from S. cerevisiae was purified by its ability to stimulate yeast DNA polymerase delta on a primed single-stranded DNA template in the presence of yeast PCNA and RPA. Like its human-cell counterpart, RFC from S. cerevisiae (scRFC) has an associated DNA-activated ATPase activity as well as a primer-template, structure-specific DNA binding activity. By analogy with the phage T4 and ...
Article Saccharomyces cerevisiae afr1 protein is a protein phosphatase 1/glc7-targeting subunit that regulates the septin cytoskeleton during mating. Glc7, the type1 serine/threonine phosphatase in the yeast Saccharomyces cerevisiae, is targeted by a...
MIG1 overexpression causes flocculation in Saccharomyces cerevisiae. An important role of glutathione and gamma-glutamyltranspeptidase in the supply of growth requirements during nitrogen starvation of the yeast Saccharomyces cerevisiae
We have isolated STN1, an essential Saccharomyces cerevisiae gene, as a suppressor of the cdc13-1 mutation. A synthetic lethal interaction between a temperature-sensitive mutant allele of STN1, stn1-13, and cdc13-1 was observed. Stn1 and Cdc13 proteins displayed a physical interaction by two-hybrid analysis. As shown previously for cdc13-1, stn1-13 cells at the restrictive temperature accumulate single-stranded DNA in subtelomeric regions of the chromosomes, but to a lesser extent than cdc13-1 cells. In addition, both Cdc13 and Stn1 were found to be involved in the regulation of telomere length, mutations in STN1 or CDC13 conferring an increase in telomere size. Loss of Stn1 function activated the RAD9 and MEC3 G2/M checkpoints, therefore confirming that DNA damage is generated. We propose that Stn1 functions in telomere metabolism during late S phase in cooperation with Cdc13 ...
Article Pet191 is a cytochrome c oxidase assembly factor in saccharomyces cerevisiae. The twin-Cx9C motif protein Pet191 is essential for cytochrome c oxidase maturation. The motif Cys residues are functionally important and appear to be present in d...
TY - JOUR. T1 - Identification of a DNA supercoiling activity in Saccharomyces cerevisiae. AU - Koo, Hyeon Sook. AU - Lau, Kawai. AU - Wu, Hai Young. AU - Liu, Leroy-Fong. PY - 1992/10/11. Y1 - 1992/10/11. N2 - A relaxed plasmid DNA is shown to become positively supercoiled in cell extracts from top1 strains of Saccharomyces cerevisiae. This positive supercoiling activity is dependent on the presence of bacterial DNA topoisomerase I and ATP (or dATP), and the positive supercoils generated in this reaction are not constrained by protein(s). Non-hydrolyzable ATP analogs cannot substitute for ATP in this supercoiling reaction, and the supercoiling activity is not due to RNA synthesis. The presence of an ARS sequence in the DNA does not alter the activity. Furthermore, this activity is equally active against UV irradiated or intact DNA. Extracts prepared from rad50 and rad52 mutant cells exhibited the same activity. Partial purification of this activity suggests that a protein factor with a native ...
Bioethanol is an alternative energy of environmentally friendly as a substitute for petroleum. Sucrose, starch, and fibrous cellulose (lignocellulose) are the main ingredients for bioethanol production. The material is very easy and abundant to get from the waste of agricultural crops. One of these agricultural wastes in Indonesia that have not been used optimally is durian seeds. Durian seeds only become waste and are not commercially useful, even though they contain high carbohydrates, which is possible as a potential new source for bioethanol production. In this work, an experimental study was conducted on bioethanol synthesis from durian seeds through fermentation by Saccharomyces cerevisiae yeast in aerobic fermenter. The process for the production of starch-based bioethanol includes milling, hydrolysis, detoxification, fermentation, and distillation. At the stage of fermentation, variations in the duration of fermentation were applied for 1-11 days. Carbohydrates contained in durian seed ...
During pretreatment of lignocellulose raw material, compounds that severely inhibit microbial activity including Saccharomyces cerevisiae strains are released [1]. These compounds, which include furaldehydes and weak organic acids, inhibit yeast metabolism and affect yeast viability and, as a consequence, reduces the overall productivity of an ethanol production process [2]. Elucidation of the molecular mechanisms behind inhibition can suggest new strategies to prevent the inhibitory effect. In the present study, the possible effect on the plasma membrane in S. cerevisiae is studied as a response to inhibitors present in lignocellulose raw material. A comparative lipidomic profiling will be carried out on S. cerevisiae cultured in the absence and presence of lignocellulose inhibitors. LC-CAD and GC-MS will be used to extensively characterize the composition of the plasma membrane. Changes in membrane composition will be correlated with the presence of specific inhibitors. References 1. Palmqvist E, Hahn
TY - JOUR. T1 - A circadian clock in Saccharomyces cerevisiae. AU - Eelderink-Chen, Zheng. AU - Mazzotta, Gabriella. AU - Sturre, Marcel. AU - Bosman, Jasper. AU - Roenneberg, Till. AU - Merrow, Martha. PY - 2010/2/2. Y1 - 2010/2/2. N2 - Circadian timing is a fundamental biological process, underlying cellular physiology in animals, plants, fungi, and cyanobacteria. Circadian clocks organize gene expression, metabolism, and behavior such that they occur at specific times of day. The biological clocks that orchestrate these daily changes confer a survival advantage and dominate daily behavior, for example, waking us in the morning and helping us to sleep at night. The molecular mechanism of circadian clocks has been sketched out in genetic model systems from prokaryotes to humans, revealing a combination of transcriptional and posttranscriptional pathways, but the clock mechanism is far from solved. Although Saccharomyces cerevisiae is among the most powerful genetic experimental systems and, as ...
en] Time-series of high throughput gene sequencing data intended for gene regulatory network (GRN) inference are often short due to the high costs of sampling cell systems. Moreover, experimentalists lack a set of quantitative guidelines that prescribe the minimal number of samples required to infer a reliable GRN model. We study the temporal resolution of data vs.quality of GRN inference in order to ultimately overcome this deficit. The evolution of a Markovian jump process model for the Ras/cAMP/PKA pathway of proteins and metabolites in the G1 phase of the Saccharomyces cerevisiae cell cycle is sampled at a number of different rates. For each time-series we infer a linear regression model of the GRN using the LASSO method. The inferred network topology is evaluated in terms of the area under the precision-recall curve (AUPR). By plotting the AUPR against the number of samples, we show that the trade-off has a, roughly speaking, sigmoid shape. An optimal number of samples corresponds to values ...
en] Time-series of high throughput gene sequencing data intended for gene regulatory network (GRN) inference are often short due to the high costs of sampling cell systems. Moreover, experimentalists lack a set of quantitative guidelines that prescribe the minimal number of samples required to infer a reliable GRN model. We study the temporal resolution of data vs.quality of GRN inference in order to ultimately overcome this deficit. The evolution of a Markovian jump process model for the Ras/cAMP/PKA pathway of proteins and metabolites in the G1 phase of the Saccharomyces cerevisiae cell cycle is sampled at a number of different rates. For each time-series we infer a linear regression model of the GRN using the LASSO method. The inferred network topology is evaluated in terms of the area under the precision-recall curve (AUPR). By plotting the AUPR against the number of samples, we show that the trade-off has a, roughly speaking, sigmoid shape. An optimal number of samples corresponds to values ...
[150 Pages Report] Check for Discount on Global and Chinese HK/Hexokinase from Saccharomyces cerevisiae (CAS 9001-51-8) Industry, 2016 Market Research Report report by Prof Research. The Global and Chinese HK/Hexokinase from Saccharomyces cerevisiae Industry,...
The yeast Saccharomyces cerevisiae strain W303 synthesizes in the early logarithmic phase of growth dolichols of 14-18 isoprene residues. The analysis of the polyisoprenoids present in the stationary phase revealed an additional family which proved to be also dolichols but of 19-24 isoprene residues, constituting 39% of the total dolichols. The transfer of early logarithmic phase cells to a starvation medium lacking glucose or nitrogen resulted in the synthesis of the longer chain dolichols. The additional family of dolichols represented 13.8% and 10.3% of total dolichols in the glucose and nitrogen deficient media, respectively. The level of dolichols in yeast cells increased with the age of the cultures. Since both families of dolichols are present in stationary phase cells we postulate that the longer chain dolichols may be responsible for the physico-chemical changes in cellular membranes allowing yeast cells to adapt to nutrient deficient conditions to maintain long-term viability ...
The budding yeast Saccharomyces cerevisiae has been considered for more than 20 years as a premier model organism for biological sciences, also being the main microorganism used in wide industrial applications, like alcoholic fermentation in the winemaking process. Grape juice is a challenging environment for S. cerevisiae, with nitrogen deficiencies impairing fermentation rate and yeast biomass production, causing stuck or sluggish fermentations, thus generating sizeable economic losses for wine industry. In the present review, we summarize some recent efforts in the search of causative genes that account for yeast adaptation to low nitrogen environments, specially focused in wine fermentation conditions. We start presenting a brief perspective of yeast nitrogen utilization under wine fermentative conditions, highlighting yeast preference for some nitrogen sources above others. Then, we give an outlook of S. cerevisiae genetic diversity studies, paying special attention to efforts in genome sequencing
The communication reports the cloning, sequencing, and analysis of the RPS3 gene from yeast, which codes for the ribosomal protein YS3. Sequence analyses of a 2.45 kb DNA fragment revealed an open reading frame with the potential to code for a 240 amino-acid long protein. The first 20 amino acids display a 90% identity to a 20 amino-acid long protein sequence of yeast ribosomal protein S3, that was obtained by protein sequencing of purified yeast ribosomal proteins. The promoter region of the RPS3 gene contains several upstream conserved sequence elements (UASrpg, T-rich region) that usually regulate transcription of ribosomal protein genes. Northern blot experiments demonstrate that this ORF is transcribed into an approximately 900 nt long mRNA. The major start site of transcription is located near position -20. The RPS3 gene is a single copy gene in yeast. Its disruption yields non viable haploid spores of Saccharomyces cerevisiae.
Bioethanol production from lignocellulosic biomass, in particular xylose, is currently of great concern, given the abundance of this sugar in the world, because Saccharomyces cerevisiae, which is widely used for bioethanol production, is unable to naturally ferment xylose. The aim of this study was to obtain a novel yeast capable of stably producing ethanol from biomass containing xylose by protoplast fusion between S. cerevisiae and xylose-utilizing yeast. We describe a novel xylose-fermenting yeast strain, FSC1, developed for ethanol production by intergeneric hybridization between S. cerevisiae and Candida intermedia mutants by using a protoplast fusion technique. The characteristics of the FSC1 strain are reported with respect to xylose fermentation, morphology, gene, and protein expression. Mutation of the parental strains prior to protoplast fusion endowed the FSC1 strain with the ability to convert xylose to ethanol. Microscopic analysis confirmed that the parental and FSC1 strains produced
Most of ethanol production processes are limited by lower ethanol production rate and recyclability problem of ethanologenic organism. In the present study, immobilized co-fermenting Saccharomyces cerevisiae GSE1618 was employed for ethanol fermentation using rice straw enzymatic hydrolysate in a packed bed reactor (PBR). The immobilization of S. cerevisiae was performed by entrapment in Ca-alginate for optimization of ethanol production by varying alginic acid concentration, bead size, glucose concentration, temperature and hardening time. Remarkably, extra hardened beads (EHB) immobilized with S. cerevisiae could be used up to repeated 40 fermentation batches. In continuous PBR, maximum 81.82 g L−1 ethanol was obtained with 29.95 g L−1 h−1 productivity with initial glucose concentration of 180 g L−1 in feed at dilution rate of 0.37 h−1. However, maximum ethanol concentration of 40.33 g L−1 (99% yield) with 24.61 g L−1 h−1 productivity was attained at 0.61 h−1 dilution rate in ...
Mitosis in yeast Saccharomyces cerevisiae was investigated in thick (0-25-I mum) serial sections with a high voltage electron microscope and in preparations of spheroplasts spread on a water surface. Spindle microtubules originate from a plaque-like structure called the spindle pole bosis the SPB duplicates and a set of long and short microtubules develops on each SPB. The spindle arises as the SPBs separate on the nuclear membrane adense and are not individually visible. Genetic studies, however, have indicated that there are 17 linkage groups. The number of microtubules was determined in diploid and haploid spindles on serial stereo micrographs. In diploid mitosis about 40 microtubules issue from a SPB. Most are non-continuous and often they are visibly associated with a chromatin fibre. The spindle in haploid cells is similar except that the number of microtubules is about half that in diploid cells and the SPB is smaller. The pole-to-pole microtubules vary in number from spindle to spindle, ...
Low fermentation temperatures are of importance to food and beverage industries working with Saccharomyces cerevisiae Therefore, the identification of genes demonstrating a positive impact on fermentation kinetics is of significant interest. A set of 121 mapped F1 progeny, derived from a cross between haploid strains BY4716 (a derivative of the laboratory yeast S288C) and wine yeast RM11-1a, were fermented in New Zealand Sauvignon Blanc grape juice at 12.5°. Analyses of five key fermentation kinetic parameters among the F1 progeny identified a quantitative trait locus (QTL) on chromosome I with a significant degree of linkage to maximal fermentation rate (Vmax) at low temperature. Independent deletions of two candidate genes within the region, FLO1 and SWH1, were constructed in the parental strains (with S288C representing BY4716). Fermentation of wild-type and deletion strains at 12.5 and 25° confirmed that the genetic linkage to Vmax corresponds to the S288C version of the FLO1 allele, as ...
In Saccharomyces cerevisiae the activity for the lactate-proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a Vmax of 2.1 nmol·s−1·mg of dry weight−1. Reconstitution of the lactate permease activity was achieved by fusing plasma membranes of P. pastoris methanol-induced cells with Escherichia coli liposomes containing cytochrome c oxidase, as proton-motive force. These assays in reconstituted heterologous P. pastoris membrane vesicles demonstrate that S. cerevisiae Jen1p is a functional lactate transporter. Moreover, a S. cerevisiae strain deleted in the JEN1 gene was transformed with a centromeric plasmid containing JEN1 under the control of the glyceraldehyde-3-phosphate dehydrogenase constitutive promotor. ...
The Saccharomyces cerevisiae SIS1 gene was identified as a high copy number suppressor of the slow growth phenotype of strains containing mutations in the SIT4 gene, which encodes a predicted serine/threonine protein phosphatase. The SIS1 protein is similar to bacterial dnaJ proteins in the amino-terminal third and carboxyl-terminal third of the proteins. In contrast, the middle third of SIS1 is not similar to dnaJ proteins. This region of SIS1 contains a glycine/methionine-rich region which, along with more amino-terminal sequences, is required for SIS1 to associate with a protein of apparent molecular mass of 40 kD. The SIS1 gene is essential. Strains limited for the SIS1 protein accumulate cells that appear blocked for migration of the nucleus from the mother cell into the daughter cell. In addition, many of the cells become very large and contain a large vacuole. The SIS1 protein is localized throughout the cell but is more concentrated at the nucleus. About one-fourth of the SIS1 protein is ...
An endonuclease from organism Saccharomyces cerevisiae, Mus81-Mms4, has been found to interact with a protein labeled Crp1 that ... Rass U, Kemper B (November 2002). "Crp1p, a new cruciform DNA-binding protein in the yeast Saccharomyces cerevisiae". Journal ... Phung HT, Tran DH, Nguyen TX (September 2020). "Saccharomyces cerevisiae". FEBS Letters. n/a (n/a): 4320-4337. doi:10.1002/1873 ... Crp1 was separately identified as a cruciform-binding protein in S. cerevisiae because it had a high affinity to target ...
Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae. Microbiology 142, 1385-1390. Mollapour, M., ... Saccharomyces cerevisiae). Hence, it is conceivable that Z. bailii puts more effort on limiting the influx of acids in order to ... The higher resistance of Z. bailii to weak acids than S. cerevisiae can partly be explained by its ability to metabolize ... Mechanism of resistance of Saccharomyces bailii to benzoic, sorbic and other weak acids used as food preservatives. Journal of ...
"Saccharomyces cerevisiae S288c CHA1". biocyc.org. Retrieved 2018-12-23. "Saccharomyces cerevisiae CHA1". pathway.yeastgenome. ... YCL064C is a gene in the genome of Saccharomyces cerevisiae, the most common species of yeast. Its function is the catalyzation ...
... is the Saccharomyces cerevisiae (a yeast) eukaryotic translation release factor. More specifically, it is the yeast ... "Sup35p [Saccharomyces cerevisiae]". National Center for Biotechnology Information, U.S. National Library of Medicine. Paushkin ...
Saccharomyces cerevisiae ferments carbohydrates into carbon dioxide and alcohol, and is used in the making of beer and bread. S ... "Saccharomyces cerevisiae - MicrobeWiki". MicrobeWiki. Retrieved 2015-11-23. "Using yeast in biology". www.yourgenome.org. ... Furthermore, research using S. cerevisiae has played a central role in understanding the mechanism of meiotic recombination and ... cerevisiae is also an important model organism, since it is a eukaryotic organism that's easy to grow. It has been used to ...
"SYP1 [Saccharomyces cerevisiae]". ncbi.nlm.nih.gov/. ncbi.nlm.nih.gov/. Retrieved 26 May 2017. Boettner DR, D'Agostino JL, ...
The Ty5 is a type of retrotransposon native to the Saccharomyces cerevisiae organism. Ty5 is one of five endogenous ... Pryde FE, Louis EJ (November 1997). "Saccharomyces cerevisiae telomeres. A review". Biochemistry Mosc. 62 (11): 1232-41. PMID ... "Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae". Genetics. 116 (1): 9- ... a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence". Genome Res. 8 (5 ...
Saccharomyces cerevisiae: the yeasts in the culture convert some of the sugars to ethanol which can undergo secondary reactions ...
Saccharomyces cerevisiae: 0.05 μg/ml - 1.6 μg/ml. *Scytalidium dimidiatum is a tinea pedis like infection resistant to most ...
"Pathway correcting DNA replication errors in Saccharomyces cerevisiae". The EMBO Journal. 12 (4): 1467-73. PMC 413358. PMID ... a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiae". Genetics. 170 (2): 569-80. doi:10.1534/genetics. ... exonuclease activity located in the DNA polymerase delta subunit of Saccharomyces cerevisiae is required for accurate ...
"Epigenetics in Saccharomyces cerevisiae." Epigenetics. 1. Cold Spring Harbor Press, 2007. Morgan, David O. (2007). The Cell ... S. cerevisiae has 16 chromosomes, S. pombe has 3. S. cerevisiae is often diploid while S. pombe is usually haploid. S. pombe ... Conversely, S. cerevisiae has well-developed peroxisomes, while S. pombe does not. S. cerevisiae has small point centromere of ... The yeast species Schizosaccharomyces pombe and Saccharomyces cerevisiae are both extensively studied; these two species ...
Zymolectins in Saccharomyces cerevisiae. Letter J. Inst. Brew., 104:298. (section 4.1) (El-Behhari et al., 2000) (Masy et al., ... In the case of "top-fermenting" ale yeast (Saccharomyces cerevisiae), the yeast creates a "kreuzen" on the top of the liquid, ... unlike "bottom-fermenting" lager yeast (Saccharomyces pastorianus) where the yeast falls to the bottom of the brewing vessel.[ ...
"MCR1 Mcr1p [Saccharomyces cerevisiae]". Entrez Gene. National Center for Biotechnology Information.. ...
Kunz BA, Ramachandran K, Vonarx EJ (April 1998). "DNA sequence analysis of spontaneous mutagenesis in Saccharomyces cerevisiae ... Heidenreich, Erich (January 2007). "Adaptive Mutation in Saccharomyces cerevisiae". Critical Reviews in Biochemistry and ... recombination has been reported to be involved in acquisition of resistance to 5-fluorocytosine in Saccharomyces cerevisiae, ... genome duplications have been found to confer resistance in S. cerevisiae to nutrient-poor environments. In the laboratory, ...
"PNP Oxidase from Saccharomyces Cerevisiae". doi:10.2210/pdb1ci0/pdb. Cite journal requires ,journal= (help) Graham, Laurie A; ...
Saccharomyces cerevisiae is generally used. These systems can check for forward and reverse mutations, as well as recombinant ...
Clarke CF, Williams W, Teruya JH (1991). "Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, ...
The yeast species Saccharomyces cerevisiae is an important model organism in cell biology. The fruiting bodies of some larger ... The yeast species Saccharomyces cerevisiae has been an important model organism in modern cell biology for much of the ... Legras JL, Merdinoglu D, Cornuet JM, Karst F (2007). "Bread, beer and wine: Saccharomyces cerevisiae diversity reflects human ... Ostergaard S, Olsson L, Nielsen J (2000). "Metabolic Engineering of Saccharomyces cerevisiae". Microbiology and Molecular ...
"Phosphate permeases of Saccharomyces cerevisiae". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1365 (1-2): 23-30. doi: ... "Phosphate permeases of Saccharomyces cerevisiae: structure, function and regulation". Biochimica et Biophysica Acta (BBA) - ...
Saccharomyces cerevisiae (top-fermenting yeast) and S. carlsbergensis (bottom-fermenting yeast). S. cerevisiae has been sold ... The budding yeast Saccharomyces cerevisiae reproduces by mitosis as diploid cells when nutrients are abundant, but when starved ... The yeast species Saccharomyces cerevisiae converts carbohydrates to carbon dioxide and alcohols in a process known as ... The term "yeast" is often taken as a synonym for Saccharomyces cerevisiae, but the phylogenetic diversity of yeasts is shown by ...
Top-fermented beers are most commonly produced with Saccharomyces cerevisiae, a top-fermenting yeast which clumps and rises to ... Many of these are not strains of brewer's yeast (Saccharomyces cerevisiae) and may have significant differences in aroma and ... The dominant types of yeast used to make beer are the top-fermenting Saccharomyces cerevisiae and bottom-fermenting ... Ostergaard, Simon; Olsson, Lisbeth; Nielsen, Jens (1 March 2000). "Metabolic Engineering of Saccharomyces cerevisiae". ...
Santangelo, G. M. (March 2006). "Glucose Signaling in Saccharomyces cerevisiae". Microbiology and Molecular Biology Reviews. 70 ...
"Processing glycosidases of Saccharomyces cerevisiae." Biochimica et Biophysica Acta (BBA)-General Subjects 1426.2 (1999): 275- ...
In Saccharomyces cerevisiae, the 5S rDNA has the unusual feature of lying inside the rDNA repeat. It is flanked by non- ... Venema, Jaap; Tollervey, David (December 1999). "Ribosome Synthesis in Saccharomyces cerevisiae". Annual Review of Genetics. 33 ... and its crystal structure in the yeast Saccharomyces cerevisiae was solved at 2.8Å resolution in 2013. Twelve of its subunits ...
Many of the relevant genes were first identified by studying yeast, especially Saccharomyces cerevisiae; genetic nomenclature ... Several gene expression studies in Saccharomyces cerevisiae have identified 800-1200 genes that change expression over the ... Analyses of synchronized cultures of Saccharomyces cerevisiae under conditions that prevent DNA replication initiation without ... December 1998). "Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray ...
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Han GS, Johnston CN, Chen X, Athenstaedt K, Daum G, Carman GM (March 2001). "Regulation of the Saccharomyces cerevisiae DPP1- ... Oshiro J, Han GS, Carman GM (November 2003). "Diacylglycerol pyrophosphate phosphatase in Saccharomyces cerevisiae". Biochimica ... "Purification and characterization of diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae". The Journal of ... "Phosphatidate phosphatases and diacylglycerol pyrophosphate phosphatases in Saccharomyces cerevisiae and Escherichia coli". ...
la fermentacion auta se debana a una temperatura de 15°C a 20°C e una levadura fòrça utilizada es Saccharomyces cerevisiae. ... es Saccharomyces pastorianus. A la fin de la fermentacion, lei levaduras tomban vèrs lo debàs, çò que li dòna son nom. Es un ...
Saccharomyces cerevisiae. 釀酒酵母 12,000,000 5,538 Caenorhabditis elegans. 秀麗隱杆線蟲 97,000,000 18,250 ...
... protein N-myristoyltransferase cause temperature-sensitive myristic acid auxotrophy in Saccharomyces cerevisiae". Proc Natl ...
This species was first described in the genus Saccharomyces as S. marxianus by the Danish mycologist, Emil Christian Hansen ... cerevisiae.[4] Studies, however, deem it to be crabtree positive which is likely due to strain differences since K. marxianus ... Kluyveromyces species are affiliated with the first of this clades while species of Saccharomyces belong to the latter. ... Both Kluyveromyces and Saccharomyces are considered a part of the "Sacchromyces complex", subclade of the Saccharomycetes.[5] ...
... 's antifungal properties has been seen with fungus such as Candida glabrata, Candida krusei, Saccharomyces cerevisiae, ...
Yeast, Saccharomyces cerevisiae 2×107 Fungus, Aspergillus nidulans 3×107 ...
Song, L. (2006). „A soluble form of phosphatase in Saccharomyces cerevisiae capable of converting farnesyl diphosphate into E,E ...
Tuntuimad pärmseened on teaduses mudelorganismina kasutatavad pagaripärm (Saccharomyces cerevisiae) ja pärmseen Candida ...
Saccharomyces cerevisiae, de 12 Mbps),[64] e en 1997 co xenoma de Escherichia coli (4,7 Mbps),[65] en 1998 co primeiro xenoma ...
In budding yeast, Saccharomyces cerevisiae, the paralogs Rad55 and Rad57 are present, which form a complex that associates with ... Shinohara A, Ogawa H, Ogawa T (May 1992). "Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like ...
Saccharomyces cerevisiae). β-glucans found in the cell walls of yeast contain a 1,3 carbon backbone with elongated 1,6 carbon ...
Burum: Saccharomyces cerevisiae (burum pobi). Cyfeiriadau[golygu , golygu cod y dudalen]. *↑ "Ensembl 2011". Nucleic Acids Res ...
... cerevisiae, the TATA box has a variable position which can range from 40 to 100 bp upstream of the start site. The TATA box is ... one study found that various Saccharomyces genomes had the consensus sequence 5'-TATA(A/T)A(A/T)(A/G)-3', yet only about 20% of ...
"The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4". The EMBO Journal. 20 ( ... "Characterization of lysine 56 of histone H3 as an acetylation site in Saccharomyces cerevisiae". The Journal of Biological ... "A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3". Proceedings of the ... of a new set of cell cycle-regulatory genes that regulate S-phase transcription of histone genes in Saccharomyces cerevisiae". ...
In yeast Saccharomyces cerevisiae, squalene epoxidase is localized to both the endoplasmic reticulum and lipid droplets. Only ...
De novo origination of a new protein-coding gene in Saccharomyces cerevisiae. Genetics. 2008, 179 (1): 487-496. PMC 2390625. ...
In the yeast Saccharomyces cerevisiae HRR is a major pathway for accurately removing psoralen-crosslinks.[23] In wild-type ... "Induction of direct repeat recombination by psoralen-DNA adducts in Saccharomyces cerevisiae: defects in DNA repair increase ...
The Institute has contributed to genome-sequencing projects of the common yeast (Saccharomyces cerevisiae, an organism which ...
Knockout or null mutations in SOD1 are highly detrimental to aerobic growth in the budding yeast Saccharomyces cerevisiae and ... In wild-type S. cerevisiae, DNA damage rates increased 3-fold with age, but more than 5-fold in mutants deleted for either the ... cerevisiae. SOD2 knockout or null mutations cause growth inhibition on respiratory carbon sources in addition to decreased post ...
The flip recombinase (or FLP) is a gene from the commonly studied yeast Saccharomyces cerevisiae which recognizes "flip ...
No lévedo Saccharomyces cerevisiae MSH4 e MSH5 actúan especificamente para facilitar sobrecruzamentos entre cromosomas ... "Cloning and characterization of the human and Caenorhabditis elegans homologs of the Saccharomyces cerevisiae MSH5 gene". ... "Cloning and characterization of the human and Caenorhabditis elegans homologs of the Saccharomyces cerevisiae MSH5 gene". ... Un mutante hipomórfico (parcialmente funcional) de MSH4 en S. cerevisiae mostrou un 30% de redución en todo o xenoma no número ...
Many different organisms are used as models for studying ALS, including Saccharomyces cerevisiae (a species of yeast),[79] ... Cellular models used to study ALS include the yeast Saccharomyces cerevisiae and rat or mouse motor neurons in culture. Small- ...
"Dissection of the assembly pathway of the proteasome lid in Saccharomyces cerevisiae". Biochemical and Biophysical Research ...
"Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks". Nature Genetics. 42 (3): 272 ...
"Identification of novel filament-forming proteins in Saccharomyces cerevisiae and Drosophila melanogaster". 》The Journal of ...
The first autophagy genes were identified by genetic screens conducted in the budding yeast Saccharomyces cerevisiae.[8][9][10] ... "Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway". The Journal of ... "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae". FEBS Lett. 333 (1-2): 169-74. doi: ... "Isolation of autophagocytosis mutants of Saccharomyces cerevisiae". FEBS Letters. 349 (2): 275-80. doi:10.1016/0014-5793(94) ...
Rajmohan R, Meng L, Yu S, Thanabalu T (April 2006). "WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta ... mutants using Saccharomyces cerevisiae". FEMS Yeast Research. 9 (8): 1226-35. doi:10.1111/j.1567-1364.2009.00581.x. PMID ...
The first autophagy genes were identified by genetic screens conducted in the budding yeast Saccharomyces cerevisiae.[8][9][10] ... Tsukada M, Ohsumi Y (October 1993). "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae ... "Lipid droplet autophagy in the yeast Saccharomyces cerevisiae". Molecular Biology of the Cell. 25 (2): 290-301. doi:10.1091/mbc ... "Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway". The Journal of ...
... complement of protein kinases of the microsporidium Encephalitozoon cuniculi in relation to those of Saccharomyces cerevisiae ...
Lethal concentrations of puromycin are much higher for strains of Saccharomyces cerevisiae than mammalian cell lines. Deletion ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Tok1p [Saccharomyces cerevisiae S288C] Tok1p [Saccharomyces cerevisiae S288C]. gi,6322368,ref,NP_012442.1, ... The TOK1 gene is conserved in S.cerevisiae, K.lactis, M.oryzae, and N.crassa. ...
The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from ... The purpose of this study was to test the hypothesis that the Saccharomyces cerevisiae transcriptome might be used as an ... S. cerevisiae transcriptomics may also be developed for testing of mixtures of conventional drugs (polypills) to discover ... Our data show that functional genomics in S. cerevisiae may be developed as a sensitive bioassay for the scientific ...
GLYCOLYSIS MUTANTS IN SACCHAROMYCES CEREVISIAE. Drago Clifton, Shelley B. Weinstock and Dan G. Fraenkel ... GLYCOLYSIS MUTANTS IN SACCHAROMYCES CEREVISIAE. Drago Clifton, Shelley B. Weinstock and Dan G. Fraenkel ... GLYCOLYSIS MUTANTS IN SACCHAROMYCES CEREVISIAE. Drago Clifton, Shelley B. Weinstock and Dan G. Fraenkel ...
Hypervariable Noncoding Sequences in Saccharomyces cerevisiae Message Subject (Your Name) has forwarded a page to you from ... Hypervariable Noncoding Sequences in Saccharomyces cerevisiae. Justin C. Fay and Joseph A. Benavides ... Hypervariable Noncoding Sequences in Saccharomyces cerevisiae. Justin C. Fay and Joseph A. Benavides ... Hypervariable Noncoding Sequences in Saccharomyces cerevisiae. Justin C. Fay and Joseph A. Benavides ...
Saccharomyces cerevisiae. Mutation(s): 0 EC: (PDB Primary Data), (UniProt). ... The structure of a new crystal form of Saccharomyces cerevisiae phosphoglycerate mutase has been solved and refined to 2.12 A ... The structure of a new crystal form of Saccharomyces cerevisiae phosphoglycerate mutase has been solved and refined to 2.12 A ... The 2.3 A X-Ray Crystal Structure of S. Cerevisiae Phosphoglycerate Mutase. Rigden, D.J., Alexeev, D., Phillips, S.E.,& ...
... plantarum NBRC 3070 and Lactobacillus casei NBRC 3425 as a ... The product under assessment is a preparation containing single strains of Saccharomyces cerevisiae, Lactobacillus plantarum ...
Gênero: Saccharomyces. Espécie: Cerevisiae. Victor William Viana Palombo - ATM 2018/2. Saccharomyces cerevisiae. Morfologia. ... Transcript of Saccharomyces cerevisiae. Domínio: Eukarya. Reino: Fungi. Sub-reino: Dikarya. Filo: Ascomycota. Sub-filo: ... S. cerevisiae. Probióticos (26/60):. Saccharomyces boulardii. -, Clostridium difficile. 60% UTI, 70%: nutrição, 17 óbitos. ...
Intelligent Bacteria - Saccharomyces cerevisiae is an artistic research project, manifested in the form of an acoustic and ... Intelligent Bacteria - Saccharomyces cerevisiae is an artistic research project, manifested in the form of an acoustic and ... Intelligent Bacteria - Saccharomyces cerevisiae, installation by HONF - The House Of Natural Fiber. ... Intelligent Bacteria - Saccharomyces cerevisiae, installation by HONF - The House Of Natural Fiber. ...
Recommended Products w/ Saccharomyces Cerevisiae Extract:. Dermalogica Pre Shave Guard, Sothys Homme Hydrating Active Care, ... Saccharomyces Cerevisiae Extract is a skin-conditioning agent used in cosmetics and beauty care products because of its ability ... Saccharomyces Cerevisiae Extract is a skin-conditioning agent used in cosmetics and beauty care products because of its ability ... Jason P. Rubin of Belli Skin Care, a derivative of Saccharomyces Cerevisiae Extract can also help to increase microcirculation ...
Saccharomyces cerevisiae) and Bacillus subtilis) on Nile tilapia (Oreochromis niloticus) reared in low input ponds. ...
Global response of Saccharomyces cerevisiae to an alkylating agent Message Subject (Your Name) has sent you a message from PNAS ... Transcriptional response of Saccharomyces cerevisiae to DNA-damaging agents does not identify the genes that protect against ... Saccharomyces cerevisiae Dap1p, a Novel DNA Damage Response Protein Related to the Mammalian Membrane-Associated Progesterone ... Global response of Saccharomyces cerevisiae to an alkylating agent. Scott A. Jelinsky and Leona D. Samson ...
Characteristic genome rearrangements in experimental evolution of Saccharomyces cerevisiae. Maitreya J. Dunham, Hassan Badrane ... Characteristic genome rearrangements in experimental evolution of Saccharomyces cerevisiae. Maitreya J. Dunham, Hassan Badrane ... Characteristic genome rearrangements in experimental evolution of Saccharomyces cerevisiae. Maitreya J. Dunham, Hassan Badrane ... Characteristic genome rearrangements in experimental evolution of Saccharomyces cerevisiae Message Subject (Your Name) has sent ...
Metabolism and Molecular Physiology of Saccharomyces Cerevisiae By J. Richard Dickinson. , Michael Schweizer. ... Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single- ... "The increasing knowledge on Saccharomyces cerevisiae and corresponding new chapters justify the need for a second edition. I ... Completely rewritten, updated, and expanded, Metabolism and Molecular Physiology of Saccharomyces cerevisiae, 2nd Edition ...
Saccharomyces +*Saccharomyces cerevisiae + * Saccharomyces cerevisiae Fleischmanns baking yeast * Saccharomyces cerevisiae 101S ... Saccharomyces cerevisiae baking yeast - Overview learn more about names for this taxon ... No one has contributed data records for Saccharomyces cerevisiae baking yeast yet. Learn how to contribute. ... Saccharomyces cerevisiae BMN1-35 * Saccharomyces cerevisiae BY2961 * Saccharomyces cerevisiae BY4741 * Saccharomyces cerevisiae ...
Invertase from Saccharomyces cerevisiae that meets the specifications developed at the fifty-seventh meeting was considered to ... be acceptable because S. cerevisiae is commonly used in the preparation of food. Its use should be limited by Good ...
... GMUS-PD-0060 $7.95 ... All about Beer & Bread (Saccharomyces cerevisiae). FACTS: For at least six thousand years, Saccharomyces cerevisiae has been ... FACTS: For at least six thousand years, Saccharomyces cerevisiae has been used to make beer and bread! (It is also one of the ... Saccharomyces cervisiae is a fungus known as Bakers & Brewers yeast because its used to make bread and ferment alcoholic ...
Saccharomyces cerevisiae S288C] H(+)-transporting V0 sector ATPase subunit d [Saccharomyces cerevisiae S288C]. gi,398366327,ref ... The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector. [J Biol Chem. ... The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector.. Bauerle C, Ho ... H(+)-transporting V0 sector ATPase subunit d [Saccharomyces cerevisiae S288C]. NCBI Reference Sequence: NP_013552.3 ...
I]Saccharomyces cerevisiae[/I] is a popular organism for industrial applications for its ethanol and thermo-tolerance, and well ... Saccharomyces cerevisiae is a popular organism for industrial applications for its ethanol and thermo-tolerance, and well ...
The DNA damage-dependent checkpoint of Saccharomyces cerevisiaeis a paradigm for eukaryotic checkpoint pathways that regulate ... Green C.M., Lowndes N.F. (2004) Purification and Analysis of Checkpoint Protein Complexes From Saccharomyces cerevisiae. In: ... The DNA damage-dependent checkpoint of Saccharomyces cerevisiae is a paradigm for eukaryotic checkpoint pathways that regulate ... A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. ...
... cerevisiae fromaround the world, the team found that the yeast species originated in China. ... an international team of researchers has generated a detailed map of genetic evolution in Saccharomyces cerevisiae, allowing ...
Control of macromolecular synthesis in Saccharomyces cerevisiae by Carl Timothy Wehr; 1 edition; First published in 1970; ... Are you sure you want to remove Control of macromolecular synthesis in Saccharomyces cerevisiae from your list? ...
... Date: Mon Feb 23 11:09:48 2004. Posted By: Michael ... It depends completely on the previous history of the organism whether S. cerevisiae or anything else. Assuming that the ...
Saccharomyces cerevisiae is used as a probiotic in humans and animals. Especially, a strain Saccharomyces cerevisiae var. ... where Saccharomyces cerevisiae becomes dormant. A variant yeast known as Saccharomyces cerevisiae var. diastaticus is a beer ... Cases of infection of oral cavity and pharynx caused by S. cerevisiae are also known. Occasionally Saccharomyces cerevisiae ... Sc2.0 or Saccharomyces cerevisiae version 2.0) aims to build an entirely designer, customizable, synthetic S. cerevisiae genome ...
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
A hormone-like substance is secreted by α mating-type cells of heterothallic yeast strains. It induces in cells of the opposite mating type, a, a morphological change characteristic of the mating process. Secretion of this substance and mating ability have some common genetic determinants. In partially purified preparations, the substance has properties of an oligopeptide. ...
Saccharomyces cerevisiae products. View information & documentation regarding Zymosan A from Saccharomyces cerevisiae
Saccharomyces cerevisiae (Candida robusta, anamorph, Cryptococcus fermentans, Saccharomyces (several species), Torula ... cerevisiae, Torulopsis fermentans, Torulopsis sexta) Classification (ascomycete/basidiomycete): Ascomycete, teleomorph ... Genus/species (aliases): Saccharomyces cerevisiae (Candida robusta, anamorph, Cryptococcus fermentans, Saccharomyces (several ... S. cerevisiae is the most common yeast used to carry out alcoholic fermentations in wine production. It is generally part of ...
... FEBS Lett. 1993 Oct 25;333(1-2):169- ...
The optimum pH range of saccharomyces cerevisiae is typically between four and six in the pH scale, as claimed by a 2005 study ... The optimum pH range of saccharomyces cerevisiae is typically between four and six in the pH scale, as claimed by a 2005 study ... Saccharomyces cerevisiae, otherwise known as bakers yeast or brewers yeast, utilizes sugar, specifically glucose, for its ... Saccharomyces cerevisiae is a fungal microorganism that thrives in an acidic environment. ...
  • A 7-month experiment was carried out to determine the effects of different levels of probiotics (baker's yeast (Saccharomyces cerevisiae) and Bacillus subtilis) on Nile tilapia (Oreochromis niloticus) reared in low input ponds. (worldfishcenter.org)
  • Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. (routledge.com)
  • Saccharomyces cervisiae is a fungus known as Baker's & Brewer's yeast because it's used to make bread and ferment alcoholic beverages, like beer and wine. (giantmicrobes.com)
  • Saccharomyces cerevisiae, otherwise known as baker's yeast or brewer's yeast, utilizes sugar, specifically glucose, for its nutritional requirements. (reference.com)
  • Saccharomyces cerevisiae, otherwise known as baker's yeast, is one of the many model organisms studied in laboratories all over the world. (jove.com)
  • The first is the addition of commercial baker's yeast, Saccharomyces cerevisiae , to dough. (frontiersin.org)
  • A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. (harvard.edu)
  • Recently, attempts have been made not only to elucidate the mechanisms by which S. cerevisiae causes vaginitis ( 5 , 8 , 19 ) but also to determine whether local or systemic predisposing factors or exposure to exogenous sources of S. cerevisiae (such as baker's yeast) may play a role in the pathogenesis of the disease ( 22 , 34 , 38 ). (asm.org)
  • The synthesis of 1,3-propanediol by biological means is extremely lucrative, and to the best of our knowledge, this is the first study focusing on the development of an optimized process for the production of the value-added chemical 1,3-propanediol from what can be considered as industrial waste, glycerol, via fermentation using instant baker's yeast ( Saccharomyces cerevisiae ). (rsc.org)
  • Saccharomyces cerevisiae (commonly known as baker's yeast) is a single-celled eukaryote that is frequently used in scientific research. (omicsonline.org)
  • Pioneering work on aging-related mechanisms has been made in Saccharomyces cerevisiae , mainly through the use of deletion collections isogenic to the S288c reference strain. (nature.com)
  • CIL:13462, Saccharomyces cerevisiae S288c. (cellimagelibrary.org)
  • Fate of highly expressed proteins destined to peroxisomes in Saccharomyces cerevisiae. (nii.ac.jp)
  • The Sec18 protein (Sec18p) of the yeast Saccharomyces cerevisiae has been identified as a component involved in the vesicular transport of proteins through the secretory and endocytotic pathways. (bl.uk)
  • This work represents two approaches taken with the aim of identifying proteins that interact with Sec18p in the yeast Saccharomyces cerevisiae . (bl.uk)
  • Saccharomyces cerevisiae is a popular organism for industrial applications for its ethanol and thermo-tolerance, and well studied genetics. (eurekalert.org)
  • S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. (omicsonline.org)
  • Completely rewritten, updated, and expanded, Metabolism and Molecular Physiology of Saccharomyces cerevisiae, 2nd Edition provides a modern account of the metabolism and physiology of this important organism. (routledge.com)
  • It depends completely on the previous history of the organism whether S. cerevisiae or anything else. (madsci.org)
  • The budding yeast Saccharomyces cerevisiae serves as an effective model organism for many cellular pathways including phosphate transport, accumulation, and storage. (scirp.org)
  • Outbreak of Saccharomyces cerevisiae subtype boulardii fungemia in patients neighboring those treated with a probiotic preparation of the organism," Journal of Clinical Microbiology , vol. 41, no. 11, pp. 5340-5343, 2003. (hindawi.com)
  • Now that we've learned a bit about S. cerevisiae as an organism, let's discuss what makes it a great model system for research. (jove.com)
  • Third, being the first eukaryotic organism to have its entire genome sequenced, S. cerevisiae has all of its gene sequences publicly available via the yeast genome database. (jove.com)
  • 1 , 2 The unicellular eukaryotic organism Saccharomyces cerevisiae has transitioned over the past decades from being one of the most widely used model organisms to elucidate fundamental cellular processes through classical biochemical or genetic approaches to becoming a model of choice to pioneer emerging disciplines such as functional genomics and systems biology. (nature.com)
  • The intestine of Polistes dominula, a social wasp, hosts S. cerevisiae strains as well as S. cerevisiae × S. paradoxus hybrids. (wikipedia.org)
  • 2016) showed that the intestine of Polistes dominula favors the mating of S. cerevisiae strains, both among themselves and with S. paradoxus cells by providing environmental conditions prompting cell sporulation and spores germination. (wikipedia.org)
  • The product under assessment is a preparation containing single strains of Saccharomyces cerevisiae , Lactobacillus plantarum and Lactobacillus casei to be used as a technological additive to improve the ensiling process. (europa.eu)
  • We studied eight strains of budding yeast ( Saccharomyces cerevisiae ) isolated after 100-500 generations of growth in glucose-limited chemostats. (pnas.org)
  • Sikorski, R. S. and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae . (springer.com)
  • Scientists developed many engineering strategies for cellulolytic S. cerevisiae strains, bringing the application of consolidated bioprocess at an industrial scale. (osti.gov)
  • The first process, the use of commercial yeast, is dominated by a small handful of S. cerevisiae strains, such as those manufactured by Fleischmann's, Red Star, and SAF. (frontiersin.org)
  • Candida albicans is more mercury resistant than S. cerevisiae and is capable of transforming inorganic mercury to methylmercury. (greenmedinfo.com)
  • The TOK1 gene is conserved in S.cerevisiae, K.lactis, M.oryzae, and N.crassa. (nih.gov)
  • DNA chip technology enables simultaneous examination of how ≈6,200 Saccharomyces cerevisiae gene transcript levels, representing the entire genome, respond to environmental change. (pnas.org)
  • The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector. (nih.gov)
  • Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. (atcc.org)
  • Here we report the design of different RNAi expression constructs to achieve a continuum of gene knockdown levels in S. cerevisiae . (aiche.org)
  • This is the first example in S. cerevisiae where RNAi is employed as a gene regulatory tool for metabolic engineering. (aiche.org)
  • Alanine : glyoxylate aminotransferase of Saccharomyces cerevisiae-encoding gene AGX1 and metabolic significance. (wikipathways.org)
  • The spectrum of mutations induced by the carcinogen N-2-acetylaminofluorene (AAF) was analysed in Saccharomyces cerevisiae using a forward mutation assay, namely the inactivation of the URA3 gene. (biomedsearch.com)
  • Other traits such as its inability to utilize lysine as a nitrogen source and a high tolerance to SO2and ethanol can be used to differentiate between S. cerevisiae and other yeasts. (ucdavis.edu)
  • Saccharomyces cerevisiae and non- Saccharomyces yeasts in grape varieties of the Sao Francisco Valley," Brazilian Journal of Microbiology , vol. 45, no. 2, pp. 411-416, 2014. (hindawi.com)
  • In light of a renewed excitement from home and commercial bakers, combined with new insights from increased worldwide sampling and whole genome sequencing, we seek to highlight the genetic basis of S. cerevisiae adaptation to dough, both in the context of commercial yeasts and sourdough starters. (frontiersin.org)
  • The purpose of this study was to test the hypothesis that the Saccharomyces cerevisiae transcriptome might be used as an indicator of phytochemical variation of closely-related yet distinctly different extracts prepared from a single species of a phytogeographically widely distributed medicinal plant. (scoop.it)
  • Saccharomyces cerevisiae (/ˌsɛrəˈvɪsi.iː/) is a species of yeast (single-celled fungus microorganisms). (wikipedia.org)
  • Shuttle vectors are usually plasmids that can propagate in two different species, such as both E. coli and S. cerevisiae. (jove.com)
  • Saccharomyces cerevisiae is the species name of yeast used for making sake. (urbansake.com)
  • In the presence of sugars, together with other essential nutrients such as amino acids, minerals and vitamins, S. cerevisiae will conduct fermentative metabolism to ethanol and carbon dioxide (as the primary fermentation metabolites) as the cells strive to make energy and regenerate the coenzyme NAD + under anaerobic conditions. (mdpi.com)
  • The vital challenges for the efficient microbial conversion of lignocellulosic biomass by engineered S. cerevisiae include heterologous expression of cellulolytic enzymes, co-fermentation of hexose and pentose sugars, and robustness against various stresses. (osti.gov)
  • article{osti_1595946, title = {Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose}, author = {Oh, Eun Joong and Jin, Yong-Su}, abstractNote = {ABSTRACT Conversion of lignocellulosic biomass to biofuels using microbial fermentation is a promising option to substitute petroleum-based production economically and sustainably. (osti.gov)
  • The yeast Saccharomyces cerevisiae has been instrumental in the fermentation of foods and beverages for millennia. (frontiersin.org)
  • This article provides computational evidence to show that functionalized magnetic nanoparticles can eliminate the wasteful oscillatory behavior in fermentation processes involving Saccharomyces cerevisiae. (scirp.org)
  • In the case of the of the Saccharomyces cerevisiae fermentation process it was shown that the addition of a little bit of oxygen would be successful in eliminating the oscillation causing Hopf bifurcations. (scirp.org)
  • The aim of this work is to incorporate the model used by [3] regarding the enhancement of oxygen mass transfer in the cybernetic Jones Kompala model [4] describing the dynamics of the Saccharomnyces cerevisiae fermentation process and demonstrate that using the functionalized magnetic nanoparticles can by altering the mass transfer coefficient actually succeed in eliminating the oscillatory behavior that plagues the Saccharomyces cerevisiae fermentation process. (scirp.org)
  • L. Sridhar, "Using Magnetic Nanoparticles to Eliminate Oscillations in Saccharomyces cerevisiae Fermentation Processes," Journal of Sustainable Bioenergy Systems , Vol. 2 No. 3, 2012, pp. 27-32. (scirp.org)
  • S. cerevisiae has many desirable traits for industrial fermentation. (frontiersin.org)
  • Ogawa, N., DeRisi, J. and Brown, P.O. (2000) New Components of a System for Phosphate Accumulation and Polyphosphate Metabolism in Saccharomyces cerevisiae Revealed by Genomic Expression Analysis. (scirp.org)
  • The optimum temperature for growth of S. cerevisiae is 30-35 °C (86-95 °F). Two forms of yeast cells can survive and grow: haploid and diploid. (wikipedia.org)
  • Saccharomyces cerevisiae yeast cells viewed under the microscope. (urbansake.com)
  • Coloured Scanning Electron Micrograph (SEM) of a colony of yeast cells, Saccharomyces cerevisiae. (sciencephoto.com)
  • The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae . (mdpi.com)
  • The crystal structure of 6-phosphogluconate dehydrogenase (6PGDH/Gnd1) from Saccharomyces cerevisiae has been determined at 2.37 A resolution by molecular replacement. (unboundmedicine.com)
  • In addition to fermentations like wine, beer, cider, sake, and bread, S. cerevisiae has been isolated from environments ranging from soil and trees, to human clinical isolates. (frontiersin.org)
  • In order to investigate the epidemiology of these vaginal infections, a total of 40 isolates of S. cerevisiae derived from symptomatic and asymptomatic women were characterized by two DNA typing approaches, named ribosomal DNA (rDNA) hybridization and Ty917 hybridization, based on the Southern blotting technique. (asm.org)
  • With the Ty917 hybridization method, all of the S. cerevisiae isolates tested appeared very heterogeneous, with the exception of those collected from individual patients with recurrent vaginitis. (asm.org)
  • This allowed us to exclude a possible common source of infection while the high relatedness among S. cerevisiae sequential isolates from recurrent-vaginitis patients could suggest a pattern of relapse rather than frequent reinfection. (asm.org)
  • S. cerevisiae cells are round to ovoid, 5-10 μm in diameter. (wikipedia.org)
  • Mother cells of the yeast S. cerevisiae reproduce asymmetrically by originating buds (daughter cells). (wiley.com)
  • In the absence of nitrogen and in the presence of a nonfermentable carbon source, Saccharomyces cerevisiae cells of the MAT a /MAT α constitution undergo meiosis, a process that results in the formation of four haploid spores. (asm.org)
  • Saccharomyces Cerevisiae Extract is a skin-conditioning agent used in cosmetics and beauty care products because of its ability to "act in oxidative stress and improve skin conditions," according to a study published in Food and Chemical Toxicology in 2008 entitled "Evaluation of dermatological effects of cosmetic formulations containing Saccharomyces cerevisiae extract and vitamins. (truthinaging.com)
  • Signalling Oxidative Stress in Saccharomyces cerevisiae, Oxidative Stress Volodymyr Lushchak and Halyna M. Semchyshyn, IntechOpen, DOI: 10.5772/34050. (intechopen.com)
  • Using Saccharomyces cerevisiae, we evaluated the main mechanisms of toxicity of two naphthoquinones, menadione and plumbagin, by determining tolerance and oxidative stress biomarkers such as GSH and GSSG, lipid peroxidation levels, as well as aconitase activity. (unboundmedicine.com)
  • Gunge, N.: Effects of elevation of strain-ploidy on transmission and recombination of mitochondrial drug resistance genes in Saccharmyces cerevisiae . (springer.com)
  • But there is no functional RNAi pathway in the eukaryotic model S. cerevisiae until recently, when a heterologous RNAi pathway from Saccharomyces castellii was reconstituted. (aiche.org)
  • Since S. cerevisiae is unable to metabolize pentose sugars, metabolic pathway engineering has been employed to introduce the respective pathways for D-xylose and L-arabinose metabolism. (rug.nl)
  • The V-ATPase of Saccharomyces cerevisiae is an ATP-dependent proton pump responsible for acidification of the vacuole and other internal compartments including the whole secretory pathway. (wiley.com)
  • The DNA damage-dependent checkpoint of Saccharomyces cerevisiae is a paradigm for eukaryotic checkpoint pathways that regulate cell cycle progression in the presence of insults to the genetic material. (springer.com)
  • NEW YORK (GenomeWeb) - As part of a flagship project begun by France Génomique, an international team of researchers has generated a detailed map of genetic evolution in Saccharomyces cerevisiae , allowing the group to better understand the population-level natural genetic and phenotypic diversity of eukaryote model systems. (genomeweb.com)
  • Gunge, N.: Genetic analysis of unequal transmission of the mitochondrial markers in Saccharmoyces cerevisiae . (springer.com)
  • Gunge, N., Nakatomi, Y.: Genetic mechanism of rare matings of the yeast Saccharomyces cerevisiae heterozygous for mating type. (springer.com)
  • Gunge, N., Tamaru, A.: Genetic analysis of products of protoplast fusion in Saccharomyces cerevisiae . (springer.com)
  • This visualization depicts the genetic interaction network of the yeast Saccharoymces cerevisiae. (visualcomplexity.com)
  • A genetic analysis of thiamine metabolism has been carried out in the budding yeast, Saccharomyces cerevisiae. (bl.uk)
  • We will review the yeast S. cerevisiae model system with emphasis on the chronological life span as a model system to study aging and the regulation of stress resistance in eukaryotes. (wiley.com)
  • Regulation of pyc1 encoding pyruvate carboxylase isozyme I by nitrogen sources in Saccharomyces cerevisiae. (wikipathways.org)
  • Here we first discuss the introduction of heterologous RNAi pathways in S. cerevisiae , and the design of various expression cassettes of RNAi precursor reagents for tunable, dynamic, and genome-wide regulation. (frontiersin.org)
  • Although vaginitis caused by Saccharomyces cerevisiae is extremely rare, in recent years we have experienced an increasing frequency of S. cerevisiae isolation from the vaginas of fertile-age women. (asm.org)
  • To download a certificate of analysis for Saccharomyces cerevisiae Meyen ex E.C. Hansen ( MYA-1830 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of Saccharomyces cerevisiae Meyen ex E.C. Hansen ( MYA-1830 ) is not currently available online. (atcc.org)
  • During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. (asm.org)
  • Herein, we hypothesized that mannan from Saccharomyces cerevisiae would dampen the characteristics of fungal allergic asthma by altering the pulmonary immune responses. (hindawi.com)
  • Along with mushrooms and molds, S. cerevisiae belongs to the Kingdom Fungi due to the presence of a cell wall made out of chitin, a polysaccharide polymer that's found not only in Fungi, but also in the exoskeletons of insects and crustaceans. (jove.com)
  • Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae . (springer.com)
  • Role of a probiotic ( Saccharomyces boulardii ) in management and prevention of diarrhoea," World Journal of Gastroenterology , vol. 12, no. 28, pp. 4557-4560, 2006. (hindawi.com)
  • Green C.M., Lowndes N.F. (2004) Purification and Analysis of Checkpoint Protein Complexes From Saccharomyces cerevisiae . (springer.com)
  • The Proteasome Subunit Rpn8 Interacts with the Small Nucleolar RNA Protein (snoRNP) Assembly Protein Pih1 and Mediates Its Ubiquitin-independent Degradation in Saccharomyces cerevisiae. (nih.gov)
  • In addition to the standard set of translation factors common in eukaryotic organisms, protein synthesis in the yeast Saccharomyces cerevisiae requires an ABCF ATPase factor eEF3, eukaryotic Elongation Factor 3. (diva-portal.org)
  • Anaerobic and sequential aerobic production of high-titer ethanol and single cell protein from NaOH-pretreated corn stover by a genome shuffling-modified Saccharomyces cerevisiae strain. (labome.org)
  • A vast amount of yeast information has been catalogued in the Saccharomyces Genome Database (SGD) and is readily accessible to anyone. (els.net)
  • Saccharomyces cerevisiae is a widely used eukaryotic model and microbial cell factory. (frontiersin.org)
  • In S. cerevisiae , phosphate is actively transported across the plasma membrane via several phosphate carriers and is then transported into the acidic vacuole (roughly equivalent to the mammalian lysosome with degradative functions but with additional storage functions, such as calcium) where it is synthesized into volutin, a storage form of polyphosphate, found in many organisms. (scirp.org)
  • Biosynthesis of beta-glucans by cell-free extracts from Saccharomyces cerevisiae. (biomedsearch.com)
  • To elucidate the impact of benzoquinone (BQ) on lipid homeostasis and cytotoxicity in Saccharomyces cerevisiae . (rsc.org)
  • C. Strassle, B. Sonnleitner and A. A. Fiechter, "A Predictive Model for the Spontaneous Synchronization of Saccharomyces cerevisiae Grown in Continuous Culture, II. (scirp.org)
  • S. cerevisiae was the first yeast to have its entire genome sequenced. (ucdavis.edu)
  • However, beer brewers slowly switched from top-fermenting (S. cerevisiae) to bottom-fermenting (S. pastorianus) yeast. (wikipedia.org)
  • FACTS: For at least six thousand years, Saccharomyces cerevisiae has been used to make beer and bread! (giantmicrobes.com)
  • Partial purification of elicitors from saccharomyces cerevisiae: role as resistance inducers in cucumber (Cucumis sativus) against colletotrichum lagenarium and as inducers of glyceollin synthesis in soybean (Glycine max). (usp.br)
  • TY - JOUR T1 - Cytotoxicity mechanism of two naphthoquinones (menadione and plumbagin) in Saccharomyces cerevisiae. (unboundmedicine.com)
  • RNA interference (RNAi) is a conserved regulatory mechanism among eukaryotes but absent from S. cerevisiae . (frontiersin.org)
  • Vaginal colonization and symptomatic vaginitis due to S. cerevisiae are extremely rare, and the incidence of vaginal infections caused by this yeast has been estimated to be less than 1% ( 23 , 24 ). (asm.org)
  • Our data show that functional genomics in S. cerevisiae may be developed as a sensitive bioassay for the scientific investigation of the interplay between phytochemical composition and transcriptional effects of complex mixtures of chemical compounds. (scoop.it)
  • We then summarize notable examples of RNAi-assisted functional genomics and metabolic engineering studies in S. cerevisiae . (frontiersin.org)
  • In the present study, the short‐term effects of two different size manufactured CuO NPs on metabolic activity of Saccharomyces cerevisiae were studied. (environmental-expert.com)
  • Exposures to CuO NPs or the released Cu2+ fraction, at doses that do not have impact on cell viability, showed significant inhibition on S. cerevisiae cellular metabolic activity. (environmental-expert.com)
  • A greater CuO NP effect on the metabolic activity of S. cerevisiae growth under respiring conditions was observed. (environmental-expert.com)
  • H. K. Von Meyenberg, "Stable Synchrony Oscillations in Continuous Culture of Saccharomyces cerevisiae under Glucose Limitation," In: B. Chance, E. K. Pye, A. K. Shosh and B. Hess, Eds. (scirp.org)
  • Assembly of subunit d (Vma6p) and G (Vma10p) and the NMR solution structure of subunit G (G(1-59)) of the Saccharomyces cerevisiae V(1)V(O) ATPase. (nih.gov)
  • The levels of total nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), and their redox states were determined as the function of growth in S. cerevisiae. (unt.edu)
  • K. D. Jones and D. S. Kompala, "Cybernetic Model of the Growth Dynamics of the Saccharomyces cerevisiae in Batch and Continuous Cultures," Journal of Biotechnology, Vol. 71, No. 1-3, 1999, pp. 105-131. (scirp.org)
  • One of the most common model organisms used to study the cell cycle is the budding yeast Saccharomyces cerevisiae. (csic.es)