A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Ribonucleic acid that makes up the genetic material of viruses.
A process that changes the nucleotide sequence of mRNA from that of the DNA template encoding it. Some major classes of RNA editing are as follows: 1, the conversion of cytosine to uracil in mRNA; 2, the addition of variable number of guanines at pre-determined sites; and 3, the addition and deletion of uracils, templated by guide-RNAs (RNA, GUIDE).
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
DNA-Directed RNA Polymerases
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
Viruses whose genetic material is RNA.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.
RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.
The processes of RNA tertiary structure formation.
RNA Polymerase II
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
RNA Processing, Post-Transcriptional
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
RNA, Small Nuclear
Short chains of RNA (100-300 nucleotides long) that are abundant in the nucleus and usually complexed with proteins in snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR). Many function in the processing of messenger RNA precursors. Others, the snoRNAs (RNA, SMALL NUCLEOLAR), are involved with the processing of ribosomal RNA precursors.
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
Nucleic Acid Conformation
Nucleic acid structures found on the 5' end of eukaryotic cellular and viral messenger RNA and some heterogeneous nuclear RNAs. These structures, which are positively charged, protect the above specified RNAs at their termini against attack by phosphatases and other nucleases and promote mRNA function at the level of initiation of translation. Analogs of the RNA caps (RNA CAP ANALOGS), which lack the positive charge, inhibit the initiation of protein synthesis.
Sequence Analysis, RNA
A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE.
Ribonucleic acid in plants having regulatory and catalytic roles as well as involvement in protein synthesis.
RNA Ligase (ATP)
DEAD-box RNA Helicases
RNA Polymerase III
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 22.214.171.124.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
RNA Polymerase I
RNA, Ribosomal, 28S
Constituent of the 60S subunit of eukaryotic ribosomes. 28S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
RNA, Ribosomal, 18S
Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
RNA, Ribosomal, 23S
The process of moving specific RNA molecules from one cellular compartment or region to another by various sorting and transport mechanisms.
RNA, Spliced Leader
The small RNAs which provide spliced leader sequences, SL1, SL2, SL3, SL4 and SL5 (short sequences which are joined to the 5' ends of pre-mRNAs by TRANS-SPLICING). They are found primarily in primitive eukaryotes (protozoans and nematodes).
Small, linear single-stranded RNA molecules functionally acting as molecular parasites of certain RNA plant viruses. Satellite RNAs exhibit four characteristic traits: (1) they require helper viruses to replicate; (2) they are unnecessary for the replication of helper viruses; (3) they are encapsidated in the coat protein of the helper virus; (4) they have no extensive sequence homology to the helper virus. Thus they differ from SATELLITE VIRUSES which encode their own coat protein, and from the genomic RNA; (=RNA, VIRAL); of satellite viruses. (From Maramorosch, Viroids and Satellites, 1991, p143)
RNA, Ribosomal, 16S
Amino Acid Sequence
Ribonucleic acid in archaea having regulatory and catalytic roles as well as involvement in protein synthesis.
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A reaction that severs one of the sugar-phosphate linkages of the phosphodiester backbone of RNA. It is catalyzed enzymatically, chemically, or by radiation. Cleavage may be exonucleolytic, or endonucleolytic.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
RNA, Heterogeneous Nuclear
RNA, Small Cytoplasmic
RNA 3' End Processing
RNA, Small Untranslated
Short RNA, about 200 base pairs in length or shorter, that does not code for protein.
RNA, Ribosomal, 5.8S
Constituent of the 60S subunit of eukaryotic ribosomes. 5.8S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
RNA, Long Noncoding
A class of untranslated RNA molecules that are typically greater than 200 nucleotides in length and do not code for proteins. Members of this class have been found to play roles in transcriptional regulation, post-transcriptional processing, CHROMATIN REMODELING, and in the epigenetic control of chromatin.
RNA, Small Nucleolar
Small nuclear RNAs that are involved in the processing of pre-ribosomal RNA in the nucleolus. Box C/D containing snoRNAs (U14, U15, U16, U20, U21 and U24-U63) direct site-specific methylation of various ribose moieties. Box H/ACA containing snoRNAs (E2, E3, U19, U23, and U64-U72) direct the conversion of specific uridines to pseudouridine. Site-specific cleavages resulting in the mature ribosomal RNAs are directed by snoRNAs U3, U8, U14, U22 and the snoRNA components of RNase MRP and RNase P.
Promoter Regions, Genetic
A family of enzymes that catalyze the endonucleolytic cleavage of RNA. It includes EC 3.1.26.-, EC 3.1.27.-, EC 3.1.30.-, and EC 3.1.31.-.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Ribonucleic acid in chloroplasts having regulatory and catalytic roles as well as involvement in protein synthesis.
Reverse Transcriptase Polymerase Chain Reaction
Single-Strand Specific DNA and RNA Endonucleases
Enzymes that catalyze the endonucleolytic cleavage of single-stranded regions of DNA or RNA molecules while leaving the double-stranded regions intact. They are particularly useful in the laboratory for producing "blunt-ended" DNA molecules from DNA with single-stranded ends and for sensitive GENETIC TECHNIQUES such as NUCLEASE PROTECTION ASSAYS that involve the detection of single-stranded DNA and RNA.
Gene Expression Regulation
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
RNA, Transfer, Phe
RNA, Transfer, Lys
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
5' Untranslated Regions
The sequence at the 5' end of the messenger RNA that does not code for product. This sequence contains the ribosome binding site and other transcription and translation regulating sequences.
RNA, Transfer, Tyr
Sequence Homology, Nucleic Acid
3' Untranslated Regions
The sequence at the 3' end of messenger RNA that does not code for product. This region contains transcription and translation regulating sequences.
Cyclic peptides extracted from carpophores of various mushroom species. They are potent inhibitors of RNA polymerases in most eukaryotic species, blocking the production of mRNA and protein synthesis. These peptides are important in the study of transcription. Alpha-amanitin is the main toxin from the species Amanitia phalloides, poisonous if ingested by humans or animals.
The functional hereditary units of VIRUSES.
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
An enzyme catalyzing the endonucleolytic cleavage of RNA at the 3'-position of a guanylate residue. EC 126.96.36.199.
Sequence Homology, Amino Acid
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Gene Expression Regulation, Viral
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Viral Nonstructural Proteins
RNA, Transfer, Amino Acyl
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
RNA Splice Sites
RNA, Transfer, Ala
A species of ENTEROVIRUS which is the causal agent of POLIOMYELITIS in humans. Three serotypes (strains) exist. Transmission is by the fecal-oral route, pharyngeal secretions, or mechanical vector (flies). Vaccines with both inactivated and live attenuated virus have proven effective in immunizing against the infection.
An RNA-containing enzyme that plays an essential role in tRNA processing by catalyzing the endonucleolytic cleavage of TRANSFER RNA precursors. It removes the extra 5'-nucleotides from tRNA precursors to generate mature tRNA molecules.
Centrifugation, Density Gradient
Viruses which produce a mottled appearance of the leaves of plants.
RNA-Directed DNA Polymerase
A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)
Open Reading Frames
Gene Expression Profiling
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
Electrophoresis, Polyacrylamide Gel
RNA, Transfer, Asp
RNA, Transfer, Met
A genus of tripartite plant viruses in the family BROMOVIRIDAE. Transmission is by beetles. Brome mosaic virus is the type species.
Proteins prepared by recombinant DNA technology.
A ribonuclease that specifically cleaves the RNA moiety of RNA:DNA hybrids. It has been isolated from a wide variety of prokaryotic and eukaryotic organisms as well as RETROVIRUSES.
Regulatory Sequences, Ribonucleic Acid
A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Cell Line, Tumor
A cell line derived from cultured tumor cells.
A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
RNA, Transfer, Gly
RNA, Transfer, His
Saccharomyces cerevisiae Proteins
RNA, Transfer, Val
A family of RNA viruses infecting insects and fish. There are two genera: Alphanodavirus and Betanodavirus.
Nucleic Acid Precursors
Use for nucleic acid precursors in general or for which there is no specific heading.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
RNA, Transfer, Arg
Sequence Analysis, DNA
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.
Escherichia coli Proteins
Proteins obtained from ESCHERICHIA COLI.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A process whereby multiple RNA transcripts are generated from a single gene. Alternative splicing involves the splicing together of other possible sets of EXONS during the processing of some, but not all, transcripts of the gene. Thus a particular exon may be connected to any one of several alternative exons to form a mature RNA. The alternative forms of mature MESSENGER RNA produce PROTEIN ISOFORMS in which one part of the isoforms is common while the other parts are different.
Ribonucleoproteins, Small Nuclear
Hepatitis Delta Virus
A defective virus, containing particles of RNA nucleoprotein in virion-like form, present in patients with acute hepatitis B and chronic hepatitis. It requires the presence of a hepadnavirus for full replication. This is the lone species in the genus Deltavirus.
Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.
RNA, Transfer, Trp
Oligonucleotide Array Sequence Analysis
Recombinant Fusion Proteins
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Trypanosoma brucei brucei
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Gene Expression Regulation, Bacterial
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A genus of plant viruses that infects ANGIOSPERMS. Transmission occurs mechanically and through soil, with one species transmitted via a fungal vector. The type species is Tomato bushy stunt virus.
The addition of a tail of polyadenylic acid (POLY A) to the 3' end of mRNA (RNA, MESSENGER). Polyadenylation involves recognizing the processing site signal, (AAUAAA), and cleaving of the mRNA to create a 3' OH terminal end to which poly A polymerase (POLYNUCLEOTIDE ADENYLYLTRANSFERASE) adds 60-200 adenylate residues. The 3' end processing of some messenger RNAs, such as histone mRNA, is carried out by a different process that does not include the addition of poly A as described here.
RNA, Transfer, Leu
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Gene silencing: plants and viruses fight it out. (1/25126)Plants can become 'immune' to attack by viruses by degrading specific viral RNA, but some plant viruses have evolved the general capacity to suppress this resistance mechanism. (+info)
Structural basis for the specificity of the initiation of HIV-1 reverse transcription. (2/25126)Initiation of human immunodeficiency virus type 1 (HIV-1) reverse transcription requires specific recognition of the viral genome, tRNA3Lys, which acts as primer, and reverse transcriptase (RT). The specificity of this ternary complex is mediated by intricate interactions between HIV-1 RNA and tRNA3Lys, but remains poorly understood at the three-dimensional level. We used chemical probing to gain insight into the three-dimensional structure of the viral RNA-tRNA3Lys complex, and enzymatic footprinting to delineate regions interacting with RT. These and previous experimental data were used to derive a three-dimensional model of the initiation complex. The viral RNA and tRNA3Lys form a compact structure in which the two RNAs fold into distinct structural domains. The extended interactions between these molecules are not directly recognized by RT. Rather, they favor RT binding by preventing steric clashes between the nucleic acids and the polymerase and inducing a viral RNA-tRNA3Lys conformation which fits perfectly into the nucleic acid binding cleft of RT. Recognition of the 3' end of tRNA3Lys and of the first template nucleotides by RT is favored by a kink in the template strand promoted by the short junctions present in the previously established secondary structure. (+info)
A premature termination codon interferes with the nuclear function of an exon splicing enhancer in an open reading frame-dependent manner. (3/25126)Premature translation termination codon (PTC)-mediated effects on nuclear RNA processing have been shown to be associated with a number of human genetic diseases; however, how these PTCs mediate such effects in the nucleus is unclear. A PTC at nucleotide (nt) 2018 that lies adjacent to the 5' element of a bipartite exon splicing enhancer within the NS2-specific exon of minute virus of mice P4 promoter-generated pre-mRNA caused a decrease in the accumulated levels of P4-generated R2 mRNA relative to P4-generated R1 mRNA, although the total accumulated levels of P4 product remained the same. This effect was seen in nuclear RNA and was independent of RNA stability. The 5' and 3' elements of the bipartite NS2-specific exon enhancer are redundant in function, and when the 2018 PTC was combined with a deletion of the 3' enhancer element, the exon was skipped in the majority of the viral P4-generated product. Such exon skipping in response to a PTC, but not a missense mutation at nt 2018, could be suppressed by frame shift mutations in either exon of NS2 which reopened the NS2 open reading frame, as well as by improvement of the upstream intron 3' splice site. These results suggest that a PTC can interfere with the function of an exon splicing enhancer in an open reading frame-dependent manner and that the PTC is recognized in the nucleus. (+info)
High level inhibition of HIV replication with combination RNA decoys expressed from an HIV-Tat inducible vector. (4/25126)Intracellular immunization, an antiviral gene therapy approach based on the introduction of DNA into cells to stably express molecules for the inhibition of viral gene expression and replication, has been suggested for inhibition of HIV infection. Since the Tat and Rev proteins play a critical role in HIV regulation, RNA decoys and ribozymes of these sequences have potential as therapeutic molecular inhibitors. In the present study, we have generated several anti-HIV molecules; a tat-ribozyme, RRE, RWZ6 and TAR decoys and combinations of decoys, and tested them for inhibition of HIV-1 replication in vitro. We used T cell specific CD2 gene elements and regulatory the HIV inducible promoter to direct high level expression and a 3' UTR sequence for mRNA stabilization. We show that HIV replication was most strongly inhibited with the combination TAR + RRE decoy when compared with the single decoys or the tat-ribozyme. We also show that the Tat-inducible HIV promoter directs a higher level of steady-state transcription of decoys and inhibitors and that higher levels of expression directly relate to increased levels of inhibition of HIV infection. Furthermore, a stabilization of the 3' end of TAR + RRE inhibitor transcripts using a beta-globin 3' UTR sequence leads to an additional 15-fold increase in steady-state RNA levels. This cassette when used to express the best combination decoy inhibitor TAR + RRE, yields high level HIV inhibition for greater than 3 weeks. Taken together, both optimization for high level expression of molecular inhibitors and use of combinations of inhibitors suggest better therapeutic application in limiting the spread of HIV. (+info)
Reverse transcription-nested polymerase chain reaction for detecting p40 RNA of Borna disease virus, without risk of plasmid contamination. (5/25126)Several methods for the detection of Borna disease virus (BDV) RNA have been reported, one being the reverse transcription-nested polymerase chain reaction (RT-nested PCR) method. However, due to the possibility of contamination of the cloned DNA in a reaction tube, false-positive results might be obtained by RT-nested PCR. To detect only BDV RNA without anxiety of contamination, we developed an RT-nested PCR system using "mRNA selective PCR kit". Using this system, cDNA of BDV p40 in the plasmid (up to 5 x 10(7) molecules) was not amplified. BDV specific sequence was amplified from total RNA (more than 50 pg) of MDCK/BDV cells, which were persistently infected with BDV. These results indicate that this mRNA selective RT-nested PCR system can specifically amplify target RNA as distinguished from plasmid contaminated. (+info)
Enteroviral RNA replication in the myocardium of patients with left ventricular dysfunction and clinically suspected myocarditis. (6/25126)BACKGROUND: Previous studies dealing with the detection of enteroviral RNA in human endomyocardial biopsies have not differentiated between latent persistence of the enteroviral genome and active viral replication. Enteroviruses that are considered important factors for the development of myocarditis have a single-strand RNA genome of positive polarity that is transcribed by a virus-encoded RNA polymerase into a minus-strand mRNA during active viral replication. The synthesis of multiple copies of minus-strand enteroviral RNA therefore occurs only at sites of active viral replication but not in tissues with mere persistence of the viral genome. METHODS AND RESULTS: We investigated enteroviral RNA replication versus enteroviral RNA persistence in endomyocardial biopsies of 45 patients with left ventricular dysfunction and clinically suspected myocarditis. Using reverse-transcriptase polymerase chain reaction in conjunction with Southern blot hybridization, we established a highly sensitive assay to specifically detect plus-strand versus minus-strand enteroviral RNA in the biopsies. Plus-strand enteroviral RNA was detected in endomyocardial biopsies of 18 (40%) of 45 patients, whereas minus-strand RNA as an indication of active enteroviral RNA replication was detected in only 10 (56%) of these 18 plus-strand-positive patients. Enteroviral RNA was not found in biopsies of the control group (n=26). CONCLUSIONS: These data demonstrate that a significant fraction of patients with left ventricular dysfunction and clinically suspected myocarditis had active enteroviral RNA replication in their myocardium (22%). Differentiation between patients with active viral replication and latent viral persistence should be particularly important in future studies evaluating different therapeutic strategies. In addition, molecular genetic detection of enteroviral genome and differentiation between replicating versus persistent viruses is possible in a single endomyocardial biopsy. (+info)
HIV-associated nephropathy is a late, not early, manifestation of HIV-1 infection. (7/25126)BACKGROUND: Human immunodeficiency virus-associated nephropathy (HIVAN) can be the initial presentation of HIV-1 infection. As a result, many have assumed that HIVAN can occur at any point in the infection. This issue has important implications for appropriate therapy and, perhaps, for pathogenesis. Since the development of new case definitions for acquired immunodeficiency syndrome (AIDS) and better tools to assess infection, the relationship of HIVAN to the time of AIDS infection has not been addressed. In this study, we reassessed the stage of infection at the time of HIVAN diagnosis in 10 patients, and we reviewed all previously published cases applying the new case definitions to assess stage of infection. METHODS: HIVAN was confirmed by kidney biopsy in HIV seropositive patients with azotemia and/or proteinuria. CD4+ cell count and plasma HIV-1 RNA copy number were measured. We also reviewed all published cases of HIVAN to determine if AIDS-defining conditions, by current Centers for Disease Control definitions, were present in patients with biopsy-proven HIVAN. RESULTS: Twenty HIV-1 seropositive patients with proteinuria and an elevated creatinine concentration were biopsied. HIVAN was the single most common cause of renal disease. CD4+ cell count was below 200/mm3 in all patients with HIVAN, fulfilling Centers for Disease Control criteria for an AIDS-defining condition. HIV-1 plasma RNA was detectable in all patients with HIVAN. In reviewing previous reports, an AIDS-defining condition was present in virtually all patients with HIVAN. CONCLUSION: HIVAN develops late, not early, in the course of HIV-1 infection following the development of AIDS. This likely accounts for the poor prognosis noted in previous publications and has implications for pathogenesis. In addition, given the detectable viral RNA levels, highly active antiretroviral therapy is indicated in HIVAN. Highly active antiretroviral therapy may improve survival as well as alter the natural history of HIVAN. (+info)
A multistate, foodborne outbreak of hepatitis A. National Hepatitis A Investigation Team. (8/25126)BACKGROUND: We investigated a large, foodborne outbreak of hepatitis A that occurred in February and March 1997 in Michigan and then extended the investigation to determine whether it was related to sporadic cases reported in other states among persons who had consumed frozen strawberries, the food suspected of causing the outbreak. METHODS: The cases of hepatitis A were serologically confirmed. Epidemiologic studies were conducted in the two states with sufficient numbers of cases, Michigan and Maine. Hepatitis A virus RNA detected in clinical specimens was sequenced to determine the relatedness of the virus from outbreak-related cases and other cases. RESULTS: A total of 213 cases of hepatitis A were reported from 23 schools in Michigan and 29 cases from 13 schools in Maine, with the median rate of attack ranging from 0.2 to 14 percent. Hepatitis A was associated with the consumption of frozen strawberries in a case-control study (odds ratio for the disease, 8.3; 95 percent confidence interval, 2.1 to 33) and a cohort study (relative risk of infection, 7.5; 95 percent confidence interval, 1.1 to 53) in Michigan and in a case-control study in Maine (odds ratio for infection, 3.4; 95 percent confidence interval, 1.0 to 14). The genetic sequences of viruses from 126 patients in Michigan and Maine were identical to one another and to those from 5 patients in Wisconsin and 7 patients in Arizona, all of whom attended schools where frozen strawberries from the same processor had been served, and to those in 2 patients from Louisiana, both of whom had consumed commercially prepared products containing frozen strawberries from the same processor. CONCLUSIONS: We describe a large outbreak of hepatitis A in Michigan that was associated with the consumption of frozen strawberries. We found apparently sporadic cases in other states that could be linked to the same source by viral genetic analysis. (+info)
The RNA synthesis machinery of negative-stranded RNA viruses (Journal Article) | SciTech Connect
The group of Negative-Stranded RNA Viruses (NSVs) includes many human pathogens, like the influenza, measles, mumps, respiratory syncytial or Ebola viruses, which produce frequent epidemics of disease and occasional, high mortality outbreaks by transmission from animal reservoirs. The genome of NSVs consists of one to several single-stranded, negative-polarity RNA molecules that are always assembled into mega Dalton-sized complexes by association to many nucleoprotein monomers. These RNA-protein complexes or ribonucleoproteins function as templates for transcription and replication by action of the viral RNA polymerase and accessory proteins. Here we review our knowledge on these large RNA-synthesis machines, including the structure of their components, the interactions among them and their enzymatic activities, and we discuss models showing how they perform the virus transcription and replication programmes. - Highlights: • Overall organisation of NSV RNA synthesis machines. • Structure and ...
Detection of negative-stranded hepatitis C virus RNA using a novel strand-specific reverse transcription-polymerase chain...
TY - JOUR. T1 - Detection of negative-stranded hepatitis C virus RNA using a novel strand-specific reverse transcription-polymerase chain reaction. AU - Mizutani, Tetsuya. AU - Ikeda, Masanori. AU - Saito, Satoru. AU - Sugiyama, Kazuo. AU - Shimotohno, Kunitada. AU - Kato, Nobuyuki. PY - 1998/2/1. Y1 - 1998/2/1. N2 - We developed a novel single-tube reverse transcription-polymerase chain reaction (RT-PCR) for the specific detection of negative-stranded hepatitis C virus (HCV) RNA. By using in vitro synthesized positive- and negative-stranded HCV RNAs, it was demonstrated that as few as 50 copies of negative-stranded HCV RNA could be specifically detected with a set of primers that amplify a 232-base pair sequence unique to the 5-non-coding region of HCV RNA, while 108 copies of positive-stranded HCV RNA were not detected. In addition, we demonstrated that this method allows the detection as few as 100 copies of negative-stranded HCV RNA even with the coexistence of a 100-fold excess of ...
EP 0832191 A4 20001115 - RECOMBINANT VIRAL NUCLEIC ACIDS
387440791 - EP 0832191 A4 2000-11-15 - RECOMBINANT VIRAL NUCLEIC ACIDS - [origin: WO9640867A1] The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systematically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates
Viruses | Free Full-Text | New Structure Sheds Light on Selective HIV-1 Genomic RNA Packaging
Two copies of unspliced human immunodeficiency virus (HIV)-1 genomic RNA (gRNA) are preferentially selected for packaging by the group-specific antigen (Gag) polyprotein into progeny virions as a dimer during the late stages of the viral lifecycle. Elucidating the RNA features responsible for selective recognition of the full-length gRNA in the presence of an abundance of other cellular RNAs and spliced viral RNAs remains an area of intense research. The recent nuclear magnetic resonance (NMR) structure by Keane et al.  expands upon previous efforts to determine the conformation of the HIV-1 RNA packaging signal. The data support a secondary structure wherein sequences that constitute the major splice donor site are sequestered through base pairing, and a tertiary structure that adopts a tandem 3-way junction motif that exposes the dimerization initiation site and unpaired guanosines for specific recognition by Gag. While it remains to be established whether this structure is conserved in the context
Poliovirüs - Vikipedi
Polivirüsün hücresel yaşam döngüsü (1) CD155 reseptörüne bağlanmasıyla başlar. Virüs endositozla alınır, ve viral RNA serbest kalır (2). Translation of the viral RNA occurs by an IRES-mediated mechanism (3). The polyprotein is cleaved, yielding mature viral proteins (4). The positive-sense RNA serves as template for complementary negative-strand synthesis, producing double-stranded replicative form (RF) RNA(5). Many positive strand RNA copies are produced from the single negative strand (6). The newly synthesized positive-sense RNA molecules can serve as templates for translation of more viral proteins (7) or can be enclosed in a capsid (8), which ultimately generates progeny virions. Lysis of the infected cell results in release of infectious progeny virions (9). ...
Repositorio da Producao Cientifica e Intelectual da Unicamp: Gene Expression From Viral Rna Genomes
This review is centered on the major strategies used by plant RNA viruses to produce the proteins required for virus multiplication. The strategies at the level of transcription presented here are synthesis of mRNA or subgenomic RNAs from viral RNA templates, and cap-snatching. At the level of translation, several strategies have been evolved by viruses at the steps of initiation, elongation and termination. At the initiation step, the classical scanning mode is the most frequent strategy employed by viruses; however in a vast number of cases, leaky scanning of the initiation complex allows expression of more than one protein from the same RNA sequence. During elongation, frameshift allows the formation of two proteins differing in their carboxy terminus. At the termination step, suppression of termination produces a protein with an elongated carboxy terminus. The last strategy that will be described is co- and/or post-translational cleavage of a polyprotein precursor by virally encoded ...
Viral Messenger RNA: Transcription, Processing, Splicing and by Joseph R. Nevins (auth.), Yechiel Becker, Julia Hadar (eds.) -...
24. -T. and Nevins, J. R. Mol. Cell. BioI. Mol. Cell. BioI. 3: 2058-2065. 25. Feldman, L. , Imperiale, M. J. and Nevins, J. R. Sci. USA 79: 4952-4956. 1982. 28. Akusjarvi, G. and Persson, H. 29. Shaw, A. R. and Ziff, E. B. Nature 290: 113-118. Nature 292: 420-426. Cell 22: 905-916. 1983. Proc. Natl. Acad. 26. Fraser, N. , Nevins, J. , Ziff, E. B. and Darnell, J. E. BioI. 129: 643-656. 1979. 27. Nevins, J. R. and Wilson, M. C. 1: J. Mol. 1981. 1981. 1980. 30. Alt, F. , Bothwell, A. L. , Koshland, M. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. J. Initiation of protein synthesis: evidence for messenger RNA independent binding of methionyl-transfer RNA to the 40 S ribosomal subunit. J. Mol. BioI. 76:379-403, 1973. , Bose, K. K. Protein synthesis in rabbit reticulocytes: characteristics of a Met-tRNAfMet binding factor. Biochem. Biophys. Res. Commun. 48:1-9, 1972. , Kyner, D. and Acs,~. Protein initiation in eukaryotes: formation and function of a ternary complex composed of a ...
Program in Virology
Upon entry, the host cell senses an invasion. Recent evidence suggests that structured viral RNAs can act as Pathogen Associated Molecular Patterns (PAMPs) that are recognized by host Pattern Recognition Receptors (PRR) to activate cell signaling. An immediate goal of the laboratory is to understand how specific viral RNA-protein interactions influence the viral life cycle and the host immune response. The triphosphate group found at the 5 end of some viral RNA transcripts (5 3P) has been described recently as an important determinant of self versus non-self that allows cells to distinguish between viral and cellular RNAs. Our results suggest that the 5 3P cannot be the only determinant because some RNAs with a 5 3P are potent activators, while others cause no activation of innate immune signaling. We have identified a non-structured region in the hepatitis C virus (HCV) 3 untranslated region RNA that activates innate immune signaling by interacting with the RNA helicase RIG-I. We ...
Link uncovered between viral RNA and human immune response
The extra length that dimer RNA provides is critical in encouraging PKR to pair up and function properly. The length needed for one PKR to bind to RNA is fifteen base pairs, said Philip Bevilacqua, professor of chemistry, Penn State, one of the lead scientists on the project along with James Cole, associate professor, University of Connecticut. To get two PKRs to bind and dimerize, you need an RNA strand that is twice as long. Coles laboratory provided evidence of dimerization of RNA and PKR. In their experiments at Penn State, the scientists found the dimer RNA activated PKR from 9 to 118 times more than the single strand RNA, depending on the RNA type. TAR RNA dimerization activated the most PKR when the TAR did not exhibit structural defects. The researchers report their findings in a recent issue of the Journal of Molecular Biology. Adding these defects decreases the number of places where PKR can bind to the RNA, Heinicke explained. RNAs that showed the greatest degree of symmetry ...
Can AFM Reveal Global Viral RNA Structure?
The genomes of RNA viruses often contain RNA structures that are crucial for translation and RNA replication and may play additional, uncharacterized roles during the viral replication cycle. RNA structure with single-nucleotide resolution. In combination with orthogonal evolutionary analyses, we uncover several conserved RNA structures in the open reading frame of the viral genome. The…
quantitative viral RNA assay - Molecular Biology
Id like to determine viral RNA level changes in virus producing cell culture transfected with as-DNA. Can anybody provide me with a protocol and anything to be noted in the operation? Thanks in advance.. ...
Nonconserved nucleotides at the 3 and 5 ends of an influenza A virus RNA play an important role in viral RNA replication. -...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
A Study to Compare the Effectiveness of Two Anti-HIV Drug Combinations - Full Text View - ClinicalTrials.gov
Patients are stratified by screening plasma viral RNA results (50,000 copies/ml or below vs above 50,000 copies/ml) and randomized to 1 of 2 treatment arms. Group 1 receives IDV 3 times daily plus d4T/3TC twice daily. Group 2 receives IDV/NFV/d4T/3TC twice daily. Patients remain on study medications for 24 weeks and are seen at the clinic once every 4 weeks after entering the study. At each clinic visit, blood samples are taken to evaluate CD4 cell count and plasma HIV RNA levels ...
Dear All, I am looking for a reliable method for the quantitation of in vitro transcribed RNA. If its quick and cheap alls the better. Also does anyone else have problems with the Ambion capped RNA making kit, Ithink they call it the mMESSAGE mMACHINE. We seem t get such variable results in term of yield its untrue, even when we make RNA from the same sample of linearised DNA (But on different days) Robert R. Woodward Email rw200 at cus.cam.ac.uk d ...
Bio-Rads New One-Step RT-ddPCR Kit for Probes Offers Precise RNA Quantitation | SelectScience
Regarding HIV following statement not TRUE DNA retrovirus
Regarding HIV following statement not TRUE DNA retrovirus
The Virus/Transcript | The Amazing World of Gumball Wiki | FANDOM powered by Wikia
[The episode starts with tons of virus organisms on Gumballs fur, then one of them stands on a hill-like lump of fur] Virus: Brothers, weve mutated many times over for this moment, but now we are ready. Today, we take this body; tomorrow, the REST OF THE WORLD! [The virus army starts cheering...
Addgene: pCAG-EGxxFP Citations
USP15 Participates in Hepatitis C Virus Propagation through Regulation of Viral RNA Translation and Lipid Droplet Formation. Kusakabe S, Suzuki T, Sugiyama Y, Haga S, Horike K, Tokunaga M, Hirano J, Zhang H, Chen DV, Ishiga H, Komoda Y, Ono C, Fukuhara T, Yamamoto M, Ikawa M, Satoh T, Akira S, Tanaka T, Moriishi K, Fukai M, Taketomi A, Yoshio S, Kanto T, Suzuki T, Okamoto T, Matsuura Y. J Virol. 2019 Mar 5;93(6). pii: JVI.01708-18. doi: 10.1128/JVI.01708-18. Print 2019 Mar 15. ...
HIV RNA Test Detects HIV After 9-11 Days
Most Obvious Whats Budding in Biology
Consequently, despite the very simple reality that these RNA species are labeled as unstable, processing of many CUTs seems for being somewhat slow. domyhomeworkfor.me DNA can be a form of nucleic acid and it truly is composed of billions of nucleotides and a few certain amino acids. These organisms reside in
Tusq X Plus பயன்பாடுகள், மருந்தளவு, பக்க விளைவுகள், நன்மைகள், தொடர்புகள் மற்றும் எச்சரிக்கைகள்
Tusq X Plus-ன் பயன்பாடுகள், மருந்தளவு, பக்க விளைவுகள், நன்மைகள், தொடர்புகள் மற்றும் எச்சரிக்கைகள் ஆகியவற்றை கண்டுபிடியுங்கள்.
Tusq X Plus பயன்பாடுகள், மருந்தளவு, பக்க விளைவுகள், நன்மைகள், தொடர்புகள் மற்றும் எச்சரிக்கைகள்
Tusq X Plus-ன் பயன்பாடுகள், மருந்தளவு, பக்க விளைவுகள், நன்மைகள், தொடர்புகள் மற்றும் எச்சரிக்கைகள் ஆகியவற்றை கண்டுபிடியுங்கள்.
EPO - T 0824/08 (Non-segmented negative strand RNA viruses/CRUCELL) of 20.11.2012
Summary of Facts and Submissions. I. European patent No. 0 846 181 with the title cDNA corresponding to the antigenome of nonsegmented negative strand RNA viruses, and process for the production of such viruses encoding additional antigenically active proteins was granted on European patent application No. 96928446.2 (published as WO 97/06270). The patent was granted with 21 claims.. II. Claim 1 of the patent as granted read as follows:. 1. A method for the production of an infectious non-segmented negative-strand RNA virus of the family Paramyxoviridae comprising. (a) introducing a cDNA molecule contained in a plasmid, wherein said cDNA molecule comprises the entire (+)-strand sequence of said negative- strand RNA virus operatively linked to an expression control sequence, which allows the synthesis of anti-genomic RNA transcripts bearing the authentic 3 -termini, and wherein said cDNA molecule consists of an integral multiple of six nucleotides, into a helper cell expressing an ...
Positive-sense single-stranded RNA virus - Wikipedia
A positive-sense single-stranded RNA virus (or (+)ssRNA virus) is a virus that uses positive sense, single-stranded RNA as its genetic material. Single stranded RNA viruses are classified as positive or negative depending on the sense or polarity of the RNA. The positive-sense viral RNA genome can also serve as messenger RNA and can be translated into protein in the host cell. Positive-sense ssRNA viruses belong to Group IV in the Baltimore classification. Positive-sense RNA viruses account for a large fraction of known viruses, including many pathogens such as the hepatitis C virus, West Nile virus, dengue virus, and SARS and MERS coronaviruses, as well as less clinically serious pathogens such as the rhinoviruses that cause the common cold. Positive-sense ssRNA viruses have genetic material that can function both as a genome and as messenger RNA; it can be directly translated into protein in the host cell by host ribosomes. The first proteins to be expressed after infection serve genome ...
Enhancement of Hepatitis C Virus RNA Replication by Cell Culture-Adaptive Mutations | Journal of Virology
In this study we developed a novel highly adapted HCV replicon that harbors two synergistic mutations in NS3 and one in NS5A. The ECF of this RNA was ∼5 × 105 CFU per μg of RNA, which is ∼20-fold higher than that of the best-adapted replicon we described recently (29). By analyzing this and several other HCV RNAs that differed with respect to their levels of cell culture adaptation, we found a clear correlation between the ECF and RNA replication as determined by two different transient-transfection assays. These results demonstrate that cell culture-adaptive mutations increase RNA replication. They also provide an explanation of how adaptive replicons are generated. As shown with the parental replicon carrying the luciferase gene, this nonadapted RNA replicated only transiently and at a low level in transfected cells. During this time, mutations must have been generated by the viral RNA polymerase that in a few instances were adaptive. By increasing the level of RNA replication, cells ...
Negative-sense single-stranded RNA virus - Wikipedia
A negative-sense single-stranded RNA virus (or (-)ssRNA virus) is a virus that uses negative sense, single-stranded RNA as its genetic material. Single stranded RNA viruses are classified as positive or negative depending on the sense or polarity of the RNA. The negative viral RNA is complementary to the mRNA and must be converted to a positive RNA by RNA polymerase before translation. Therefore, the purified RNA of a negative sense virus is not infectious by itself, as it needs to be converted to a positive sense RNA for replication. These viruses belong to Group V on the Baltimore classification. In addition, negative-sense single-stranded RNA viruses have complex genomic sequences, cell cycles, and replication habits that use various protein complexes to arrange in specific conformations and carry out necessary processes for survival and reproduction of their genomic sequences. The complexity of negative-sense single-stranded RNA viruses carries into its ability to suppress the innate immune ...
Importance of the positive-strand RNA secondary structure of a murine coronavirus defective interfering RNA internal...
TY - JOUR. T1 - Importance of the positive-strand RNA secondary structure of a murine coronavirus defective interfering RNA internal replication signal in positive-strand RNA synthesis. AU - Repass, John F.. AU - Makino, Shinji. PY - 1998/10. Y1 - 1998/10. N2 - The RNA elements that are required for replication of defective interfering (DI) RNA of the JHM strain of mouse hepatitis virus (MHV) consist of three discontinuous genomic regions: about 0.46 to 0.47 kb from both terminal sequences and an internal 58-nucleotide (nt)-long sequence (58-nt region) present at about 0.9 kb from the 5 end of the DI genome. The internal region is important for positive-strand DI RNA synthesis (Y. N. Kim and S. Makino, J. Virol. 69:4963-4971, 1995). We further characterized the 58-nt region in the present study and obtained the following results. (i) The positive-strand RNA structure in solution was comparable with that predicted by computer modeling. (ii) Positive-strand RNA secondary structure, but not ...
Coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in RNA synthesis. | Journal of...
Both genomic and subgenomic replicative intermediates (RIs) and replicative-form (RF) structures were found in 17CL1 mouse cells that had been infected with the A59 strain of mouse hepatitis virus (MHV), a prototypic coronavirus. Seven species of RNase-resistant RF RNAs, whose sizes were consistent with the fact that each was derived from an RI that was engaged in the synthesis of one of the seven MHV positive-strand RNAs, were produced by treatment with RNase A. Because the radiolabeling of the seven RF RNAs was proportional to that of the corresponding seven positive-strand RNAs, the relative rate of synthesis of each of the MHV positive-strand RNAs may be controlled by the relative number of each of the size classes of RIs that are produced. In contrast to alphavirus, which produced its subgenome-length RF RNAs from genome-length RIs, MHV RF RNAs were derived from genome- and subgenome-length RIs. Only the three largest MHV RF RNAs (RFI, RFII, and RFIII) were derived from the RIs that ...
NMR structure of stem-loop SL2 of the HIV-1 Ψ RNA packaging signal reveals a novel A-U-A base-triple platform<...
TY - JOUR. T1 - NMR structure of stem-loop SL2 of the HIV-1 Ψ RNA packaging signal reveals a novel A-U-A base-triple platform. AU - Amarasinghe, Gaya K.. AU - De Guzman, Roberto N.. AU - Turner, Ryan B.. AU - Summers, Michael F.. PY - 2000/5/26. Y1 - 2000/5/26. N2 - The genome of the human immunodeficiency virus type-1 (HIV-1) contains a stretch of ~120 nucleotides known as the ψ-site that is essential for RNA packaging during virus assembly. These nucleotides have been proposed to form four stem-loops (SL1-SL4) that have both independent and overlapping functions. Stem-loop SL2 is important for efficient recognition and packaging of the full-length, unspliced viral genome, and also contains the major splice-donor site (SD) for mRNA splicing. We have determined the structure of the 19-residue SL2 oligoribonucleotide by heteronuclear NMR methods. The structure is generally consistent with the most recent of two earlier secondary structure predictions, with residues G1-G2-C3-G4 and C6-U7 forming ...
ASMscience | Poliovirus RNA Replicati
Poliovirus RNA replicates in membrane-associated replication complexes in the cytoplasm of infected cells. By using a reversible inhibitor of poliovirus RNA replication, it is possible to synchronize viral RNA replication. The processing of the viral polyprotein results in the formation of the individual viral proteins along with stable intermediates in the processing pathway. To expand the utility of the in vitro complementation assay, experiments were designed to determine if all of the viral replication proteins could be provided in trans to support the replication of mutant RNA templates. The authors engineered two transcript RNAs (DJB2 and DJB15) that contained large out-of-frame deletions in the polyprotein coding sequence. The results to date using the in vitro complementation assay indicate that the 5 cloverleaf, the 3 nontranslated region (NTR), and the poly(A) tail are the minimum sequences required for negative-strand synthesis. Previous studies have shown that the 5 cloverleaf plays an
Umbravirus - Positive Sense RNA Viruses - Positive Sense RNA Viruses (2011) - International Committee on Taxonomy of Viruses ...
Figure 2 shows the genome organization of GRV; those of other umbraviruses are very similar. For each RNA, there is at the 5′ end a very short non-coding region preceding ORF1, which encodes a putative product of 31-37 kDa. ORF2, which slightly overlaps the end of ORF1, could encode a product of 63-65 kDa but lacks an AUG initiation codon near its 5′ end. However, immediately before the stop codon of ORF1 there is a 7 nt sequence that is associated with frameshifting in several plant and animal viruses, and it seems probable that ORF1 and ORF2 are translated as a single polypeptide of 94-98 kDa by a mechanism involving a −1 frameshift. The predicted product contains, in the ORF2 region, sequence motifs characteristic of viral RdRp. A short untranslated region separates ORF2 from ORF3 and ORF4, which overlap each other almost completely in different reading frames and each yield a putative product of 26-29 kDa. The ORF4 product contains sequences characteristic of plant virus MPs. The ORF3 ...
Molecular cloning of full-length HIV-1 genomes directly from plasma viral RNA<...
TY - JOUR. T1 - Molecular cloning of full-length HIV-1 genomes directly from plasma viral RNA. AU - Fang, Guowei. AU - Weiser, Barbara. AU - Visosky, Aloise A.. AU - Townsend, Laura. AU - Burger, Harold. PY - 1996. Y1 - 1996. N2 - Human immunodeficiency virus type 1 (HIV-1) in plasma reflects the replicating virus population at any point in time in vivo. Studies of the relationship of the complete HIV-1 genome to pathogenesis therefore need to focus on plasma virions. Since dual infections and recombination can occur in vivo, cloning an intact plasma virus genome as a single full-length molecule is desirable. For these reasons, we developed an efficient method to clone full-length HIV-1 genomes directly from plasma viral RNA. This method used reverse transcription and long polymerase chain reaction (PCR) amplification. Virion-associated RNA was isolated from plasma samples and then reverse- transcribed to make cDNA for PCR amplification. Two different strategies were employed to amplify the ...
RNA replication protein elisa and antibody
Hepatitis C trojan (HCV) is an enveloped, positive strand RNA computer | Selective Sirt2 inhibition by ligand-induced...
Hepatitis C trojan (HCV) is an enveloped, positive strand RNA computer virus of about 9. attributable to inherently different properties of low density particles, to association of these particles with factors stimulating fusion, or to co-floatation of factors enhancing fusion activity in genus of the Flaviviridae family (1). Based on sequence comparison, patient isolates are classified into seven genotypes, differing in their nucleotide sequence by 30C35% (2C5). The two viral surface proteins, E1 (residues 192C383) and E2 (residues 384C746), are processed by transmission peptidases of the endoplasmic reticulum from a 3,000-amino acid-long polyprotein encoded by the HCV genome (examined in Ref. 2). The E1 (31 kDa) and E2 (70 kDa) proteins are glycosylated in their large amino-terminal ectodomains (6) and are anchored in the viral membrane by their carboxyl-terminal transmembrane domains. E1 and E2 form a heterodimer stabilized by noncovalent interactions. This oligomer is usually thought to be ...
Mechanism of RNA synthesis initiation by the vesicular stomatitis virus polymerase | The EMBO Journal
Early biochemical experiments established that the minimal RNA synthesis machinery of NNS RNA viruses comprises the N encased genomic RNA associated with the viral polymerase, an L-P complex (Emerson and Yu, 1975; Mellon and Emerson, 1978). The atomic structure of N‐RNA complexes from VSV and rabies virus provided evidence that the RNA must somehow be dissociated from N for copying by the polymerase (Albertini et al, 2006; Green et al, 2006). The co‐crystal structure of the PCTD of VSV with the N‐RNA complex led to a model in which P brings L to the RNA template by binding directly between N molecules, and this interaction is perhaps also required to keep L associated with the N‐RNA during copying (Green and Luo, 2009). By now providing the first direct evidence that L can actually use RNA in the absence of the N and P, we have defined the minimal RNA synthesis components as L and RNA. We conclude that while N and P play important roles in viral RNA synthesis they are not essential for ...
Exploiting and exploring the interactions between microRNA-122 and Hepatitis C virus
Hepatitis C virus (HCV) is a single-stranded plus-sense RNA virus that is transmitted by blood-to-blood contact, and infects the human liver. HCV has a unique dependence on the liver-specific microRNA miR-122, where miR-122 binds the 5´ un-translated region of the viral RNA at two tandem sites and increases viral RNA abundance. The mechanisms of augmentation are not yet fully understood, but the interaction is known to stabilize the viral RNA, increase translation from the viral internal ribosomal entry site (IRES), and result in increased viral yield. In an attempt to create a small animal model for HCV, we added miR-122 to mouse cell lines previously thought non-permissive to HCV, which rendered these cells permissive to the virus, additionally showing that miR-122 is one of the major determinants of HCV hepatotropism. We found that some wild-type and knockout mouse cell lines - NCoA6 and PKR knockout embryonic fibroblasts - could be rendered permissive to transient HCV sub-genomic, but not ...
Hepatitis C Virus Translation Preferentially Depends on Active RNA Replication - pdf descargar
Hepatitis C Virus Translation Preferentially Depends on Active RNA Replication. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
HCV Research and News: August 2010
The HCV replication complex. After clathrin-mediated endocytosis, fusion of HCV with cellular membranes, and uncoating the viral nucleocapsid, the single-stranded positive-sense RNA genome of the virus of approximately 9600 nucleotides is released into the cytoplasm to serve as a messenger RNA for the HCV polyprotein precursor. The HCV genome contains a single large open reading frame encoding for a polyprotein of approximately 3100 amino acids. The translated section of the HCV genome is flanked by the strongly conserved HCV 3′ and 5′ untranslated regions (UTR). The 5′ UTR is comprised of four highly structured domains forming the internal ribosome entry site (IRES), which is a virus-specific structure to initiate HCV mRNA translation. From the initially translated polyprotein, the structural HCV protein core (C) and envelope 1 and 2 (E1, E2); p7; and the six nonstructural HCV proteins NS2, NS3, NS4A, NS4B, NS5A and NS5B, are processed by both viral and host proteases. The core protein ...
William Tapprich | Biology | University of Nebraska Omaha
The overall goal of our research is to understand the structure and function of RNA molecules. Most of our early work focused on ribosomal RNA (rRNA), characterizing the role of the RNA in protein synthesis (Vila et al 1994, Thompson et al 2001). More recently, we have turned our attention to understanding the structure and function of viral RNA molecules, particularly enteroviral genomic RNA. We have conducted studies to learn the structure of the internal ribosome entry site (IRES) RNA found in picornaviruses (Kim et al., 2005, Bailey and Tapprich 2007) and we have determined virulence determinants in picornal genomic RNA (Prusa et al. 2014). In our initial studies we have used chemical modification and primer extension to deduce the structure of the IRES elements in coxsackievirus B3. This analysis has been completed for virulent wild type viruses, attenuated mutant viruses and avirulent viruses. We have shown localized structural changes in the IRES RNA that correlate with virulence. These ...
Viruses | Free Full-Text | Biogenesis and Dynamics of the Coronavirus Replicative Structures | HTML
Coronaviruses are positive-strand RNA viruses that are important infectious agents of both animals and humans. A common feature among positive-strand RNA viruses is their assembly of replication-transcription complexes in association with cytoplasmic membranes. Upon infection, coronaviruses extensively rearrange cellular membranes into organelle-like replicative structures that consist of double-membrane vesicles and convoluted membranes to which the nonstructural proteins involved in RNA synthesis localize. Double-stranded RNA, presumably functioning as replicative intermediate during viral RNA synthesis, has been detected at the double-membrane vesicle interior. Recent studies have provided new insights into the assembly and functioning of the coronavirus replicative structures. This review will summarize the current knowledge on the biogenesis of the replicative structures, the membrane anchoring of the replication-transcription complexes, and the location of viral RNA synthesis, with particular
Probing the HIV-1 genomic RNA trafficking pathway and dimerization by genetic recombination and single virion analyses. -...
Once transcribed, the nascent full-length RNA of HIV-1 must travel to the appropriate host cell sites to be translated or to find a partner RNA for copackaging to form newly generated viruses. In this report, we sought to delineate the location where HIV-1 RNA initiates dimerization and the influence of the RNA transport pathway used by the virus on downstream events essential to viral replication. Using a cell-fusion-dependent recombination assay, we demonstrate that the two RNAs destined for copackaging into the same virion select each other mostly within the cytoplasm. Moreover, by manipulating the RNA export element in the viral genome, we show that the export pathway taken is important for the ability of RNA molecules derived from two viruses to interact and be copackaged. These results further illustrate that at the point of dimerization the two main cellular export pathways are partially distinct. Lastly, by providing Gag in trans, we have demonstrated that Gag is able to package RNA from either
This observation argues that RNPs are not randomly incorporated into virions, and is consistent with the presence of specific signals in each RNA segment that enable the RNPs to be packaged as a complete set. The mechanisms by which these signals are recognized, and how they ensure incorporation of one copy of each RNA segment into the particle, are not known.. There is clear evidence for a selective mechanism during the packaging of the bacteriophage ψ6 genome. Viral particles contain one copy each of a S, M, and L dsRNA segment. All particles contain a complete complement of genome segments, as indicated by the fact that every virus particle is infectious. Only the S RNA segment can enter newly formed particles; once that segment is packaged, then the M RNA can enter. The L RNA can only enter particles that contain both the S and M segments. Precise packaging is therefore the result of a serial dependence of packaging of the RNA segments.. Muramoto, Y., Takada, A., Fujii, K., Noda, T., ...
Viral RNA-dependent RNA Polymerase Assay. The Viral RNA-dependent RNA Polymerase Assay is developed using a RNA polymerase in the Flaviviridae,. a family of positive, single-stranded, enveloped RNA viruses. The assay is based on measurement of the. RNA molecules synthesized by the RNA polymerase using RNA as a template in the presence of NTPs.. The assay can be performed in a 384-well or 96-well plate format for tests of theenzyme activities of RNA. polymerases in the Flaviviridae family and high throughput screening of inhibitors. ...
PROTEIN AND RNA SYNTHESIS INHIBITION BY VESICULAR STOMATITIS VIRUS: A by DAVID DEEDS DUNIGAN
Protein and RNA synthesis are inhibited when VSV infects certain cells. UV-inactivation analysis of the virus indicates that transcription of two regions of the viral genome are required for efficient inhibition. The larger of the two viral products represents transcription of approximately 1500 nucleotides and may represent the N protein gene, while the smaller product is approximately 40 nucleotides long. The latter product is thought to be encoded at the 3-proximal end of the genome.^ Two viral mutants have been shown to be deficient in the expression of the smaller transcription product and result in less efficient inhibition of both protein and RNA synthesis. Analysis of these mutants and the UV-inactivated wild-type virus have allowed for the establishment of conditions where the effects of either the large or the small transcription product can be observed independent of the other. This will allow for the correlation of a viral product with inhibition, and thereby establish the causative
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My group uses X-ray crystallography as a central technique to study the structure-function relationships of complexes involving RNA of various kinds in eukaryotic cells. This includes the transcription/replication machinery of segmented negative strand RNA viruses (e.g. influenza), complexes involved in sorting of Pol II transcripts into their appropriate processing pathways and innate immune system pattern recognition receptors, notably the response to viral RNA via Rig-I like helicases.. Keywords: Protein-RNA recognition / aminoacyl tRNA synthetases / RNA metabolism / virus structure / influenza virus polymerase / innate immunity / Rig-I like helicases / X-ray crystallography. Subject area(s): Microbiology, Virology & Pathogens , RNA , Structural Biology & Biophysics. ...
Role Of Rna Protein Interactions In The Internal Initiation Of Translation Of Plus Strand Rna Viruses Novel Target For...
role of rna-protein interactions in the internal initiation of translation of plus-strand rna viruses : a novel target for antiviral therapeutics
A Study of Two Methods of Determining When to Begin or Change Anti-HIV Treatment - Full Text View - ClinicalTrials.gov
Although changing therapies is a common strategy in the treatment of HIV disease, guidelines are needed to help clinicians and patients decide when a change in antiretroviral therapy is indicated. The technology of measuring HIV RNA in plasma has been suggested as a tool for monitoring clinical drug efficacy. However, uncertainty remains about whether aggressive antiretroviral treatment to lower HIV RNA and maintain low levels for as long as possible will confer clinical benefit in comparison with management based on monitoring CD4 counts and HIV-related symptoms.. Patients are randomized to a decision making strategy for initiating or changing therapy based on current clinical practice alone vs. decision making based on plasma HIV RNA quantitation in addition to current clinical practice in patients with ,= 300 CD4+ cells/mm3. All patients in the RNA arm as well as a subset (n = 183) of those in the CCP arm will have a plasma HIV RNA quantitation drawn every 4 months. The results of these ...
RNase-Resistant Virus-Like Particles Containing Long Chimeric RNA Sequences Produced by Two-Plasmid Coexpression System |...
The armored L-RNA (2,248 bp) expressed by our two-plasmid coexpression system differs in several respects from the virus-like particles previously described by Pickett and Peabody (17). These authors also used a two-plasmid expression system; their goal was to determine whether the 21-nt Operator (pac site) would confer MS2-specific packageability on nonbacteriophage RNA in vivo. The E. coli was induced such that the Operator-lacZ hybrid RNA was coexpressed with the MS2 coat protein. The specificity of the Pickett and Peabody bacteriophage packaging system, however, was poor since the host E. coli RNA was packaged in preference to the Operator-lacZ RNA. In other studies, Pickett and Peabody modified the packaging of the Operator-lacZ RNA by changing the ratios of coat protein to Operator-lacZ RNA produced in E. coli. By increasing the concentration of the Operator-lacZ RNA and decreasing the concentration of the coat protein, these researchers were able to encapsulate mainly the Operator-lacZ ...
Bunyaviridae - microbewiki
The bunyavirus genome is monomeric and consists of three segments of linear negative-sense (or ambisense, depending on the genus) RNA. The terminal sequences of each segment are base-paired. Because of this, the RNAs form non-covalently closed circles. The nucleotide sequences at the 3-terminus and the 5-terminus are complimentary, forming panhandle structures. The 5-terminus is not capped. The complete genome is 10500-22700 nucleotides long. The three segments of the genome are labeled L, M, and S. The L segment is 6300-12000 nucleotides long and encodes the viral RNA polymerase. The M segment is 3500-6000 nucleotides long and encodes two glycoproteins as a single gene product that is usually co-translationally cleaved. The S segments is 1000-2200 nucleotides long and encodes the coat protein. (sources: Descriptions of Plant Viruses, ICTVdB) ...
Ultrastructure of (+)RNA virus replication organelles | EUVIRNA
Genome RNA replication of all (+)RNA viruses takes place in close association with rearranged intracellular membranes. We are only beginning to understand the biogenesis and ultrastructure of these virus-induced membrane structures. In collaboration with the virology groups of LUMC (Prof. Dr. Eric Snijder) and the University of Utrecht (Prof. Dr. Frank van Kuppeveld), EM and tomography approaches will be used to gain more insight into the architecture of the rearranged membranes, the localization of the viral replication enzymes, and the localization of host factors that are hijacked by picornavirus to facilitate replication of their RNA genome.. Host institute ...
Influenza viral RNA synthesis
Once the (-) strand influenza viral RNAs enter the nucleus, they serve as templates for the synthesis of mRNAs. These molecules are then transported back to the cytoplasm, where they direct the synthesis of viral proteins. However, the mRNAs are not complete copies of the viral (-) strand RNAs - they are missing sequen
Frontiers | A Robust and Efficient Numerical Method for RNA-Mediated Viral Dynamics | Applied Mathematics and Statistics
The multiscale model of hepatitis C virus (HCV) dynamics, which includes intracellular viral RNA (vRNA) replication, has been formulated in recent years in order to provide a new conceptual framework for understanding the mechanism of action of a variety of agents for the treatment of HCV. We present a robust and efficient numerical method that belongs to the family of adaptive stepsize methods and is implicit, a Rosenbrock type method that is highly suited to solve this problem. We provide a Graphical User Interface that applies this method and is useful for simulating viral dynamics during treatment with anti-HCV agents that act against HCV on the molecular level.
miRNA Entry for MI0007613
Recent studies have shown that replication of hepatitis C virus (HCV) is dependent on miR-122 expression. miR-122 regulates HCV by binding directly to two adjacent sites close to the 5 end of HCV RNA. Although these experiments were conducted using genotype 1a and 1b HCV RNA, the miR-122 binding sites are highly conserved across different genotypes, and miR-122 is also required for replication of infectious type 2a HCV. As miRNAs generally function to repress gene expression by binding to 3UTR sites, this positive regulation of viral replication via a 5UTR represents a novel function for miR-122. The mechanism of regulation is not yet clear. miR-122 stimulates translation of HCV RNA, but not to a sufficient extent to explain its effects on viral replication, indicating that a second stage of the viral replication cycle must also be regulated. HCV RNA synthesis is not affected by miR-122, suggesting that regulation of other processes such as RNA stability may occur. ...
Hiv rna pcr test - Things You Didnt Know
Hiv rna pcr test - What is the window period for p42 antigen/ antibody dual 4th gen HIV test? And the window period for HIV RNA PCR test? When can it be conclusive? See below. Please consult this site for a detailed answer. Nucleic acid tests are conclusive about a month after acquiring infection. Http://www. Sfaf. Org/hiv-info/testing/hiv-test-window-periods. Html
How Accurate Is The Hiv Rna Test - The Body
PA - Polymerase acidic protein - Influenza A virus (strain A/Swine/Wisconsin/1/1961 H1N1) - PA gene & protein
Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5 cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
Emory ETDs : Effects of Life History and Genome Architecture on ssRNA Virus Evolution and Extinction
Single-stranded RNA viruses have evolved to survive extremely high mutation rates.The ubiquity and effect of ssRNA viral diseases makes an understanding of the theoretical and mechanical underpinnings of rapid viral evolution vital to our ability to control them. In this body of work, we explore some of the ways in which ssRNA viruses can uncouple the rate at which variation is generated (mutation rate) from the rate at which variation is observed (measured rate of molecular evolution).. ...
RNA Replicase - RNA Dependent RNA Polymerase Summary | CureHunter Mobile
Description: An enzyme that catalyses RNA-template-directed extension of the 3- end of an RNA strand by one nucleotide at a time, and can initiate a chain de novo. (Enzyme Nomenclature, 1992, p293 ...
Hepatitis C Viral RNA, Quantitative, Real-Time PCR Lab Test
Order the hepatitis C viral RNA, quantitative, real-time PCR lab test online from the convenience of your home and without visiting a doctor!
DNA/RNA Synthesis | Cell Cycle|DNA Damage| CSNpharm
Nucleic acids are polymers of nucleotides, including DNA and RNA. DNA/RNA synthesis is an anabolic mechanism generally involving the chemical reaction of phosphate, pentose sugar, and a nitrogenous base. Certina enzymes are required to facilitate the event. Defects or deficiencies in these enzymes can lead to a variety of diseases, and a lot of chemicals that affect DNA/RNA synthesis are used in diseases relating to cell proliferation, such as cancers and microbial infections.
CIM seminar: Mechanisms of activation of innate immune responses in viral RNA and virus infection stimulated human cells |...
Ilkka Julkunen, Department of Virology, University of Turku and Virology Unit, National Institute for Health and Welfare (THL), Helsinki, Finland, will give a seminar on Mechanisms of activation of innate immune responses in viral RNA and virus infection stimulated human cells Host: Malin Flodström Tullberg
Loop de loop: viral RNA evades IFIT1 targeting - UQ eSpace
مرور Volume 5, Issue بر اساس عنوان
Introduction: The SARS-COV-2 is a non-segmented positive-sense RNA virus that belongs to the genus Beta Coronavirus. The envelope-anchored trimeric spike protein on the virus surface is considered to be the key protein for ...
Arginine and its effects on viral replication - BioCeuticals
BIO-SYNTHESIS - Custom Antibody Custom Peptide Synthesis Custom SiRNA Synthesis: RNA Transcription
RNA is chemically better suited to carry out certain tasks than is DNA. There are also other reasons RNA, not DNA, is used for these tasks. First, it is desirable to keep DNA available for replication and not tied up with other functions. Second, the small number of DNA molecules in the cell is often insufficient. Creating many identical RNA molecules that are copies of a single segment of DNA provides the necessary numbers. Third, RNA can be differentially degraded when it is no longer needed, providing an important regulatory mechanism that would be unavailable if there were only one type of nucleic acid. ...
Ampliqon produces innovative solutions supplementing DNA and RNA extraction. We have recently launched a brand new product, named G2 DNA/RNA Enhancer. G2 DNA/RNA Enhancer increases the DNA yield from difficult samples such as clay dramatically. G2 DNA/RNA Enhancer must be used either in combination with commercial extraction kits or as part of the extraction method of interest.. ...
Detailed view of viral replication machinery lends new insights into infection
RNA extraction using trizol/tri - OpenWetWare
RNA - QIAGEN
This section describes considerations for isolation and quantification of RNA from different sample sources. It also discusses the best practices for RNA, including how to store and stabilize RNA samples, how to disrupt and homogenize samples to get the highest yield, how to perform common RNA calculations, and how to use advanced technologies for RNA sample QC.
RNA - QIAGEN
This section describes considerations for isolation and quantification of RNA from different sample sources. It also discusses the best practices for RNA, including how to store and stabilize RNA samples, how to disrupt and homogenize samples to get the highest yield, how to perform common RNA calculations, and how to use advanced technologies for RNA sample QC.
Robyn S. Klein
Neuroinflammation During RNA Viral Infections. Klein RS, Garber C, Funk KE, Salimi H, Soung A, Kanmogne M, Manivasagam S, Agner ... CCR5 limits cortical viral loads during West Nile virus infection of the central nervous system. Durrant DM, Daniels BP, ... Another goal of the Klein lab is to understand how glial cells regulate T cell activity in viral infections and autoimmune ... CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis. Funk KE, Klein RS. Journal of ...
Viral replication is cytoplasmic. Replication follows the positive-strand RNA virus replication model. Positive-strand RNA ... The genome has one open reading frame which encodes the RNA-dependent RNA polymerase (RdRp). The genome is associated with the ... The genus has two species: Narnaviridae Narnavirus Saccharomyces 20S RNA narnavirus Saccharomyces 23S RNA narnavirus ICTV. " ... "Viral Zone". ExPASy. Retrieved 15 June 2015. Espino-Vázquez AN, Bermúdez-Barrientos JR, Cabrera-Rangel JF, Córdova-López G, ...
Bacteriophage experimental evolution
Viral escape from antisense RNA. Mol. Microbiol. 28:835-846. Hibma, A. M., S. A. Jassim, and M. W. Griffiths. 1997. Infection ... Viral escape from Merril, C. R., B. Biswas, R. Carlton, N. C. Jensen, G. J. Creed, S. Zullo, and S. Adhya. 1996. Long- ... Evolvability of an RNA virus is determined by its mutational neighbourhood. Nature 406:625-628. Wichman, H. A., L. A. Scott, C ... Altered 3'-terminal RNA structure in phage Q_ adapted to host factor-less Escherichia coli. Proc. Natl. Acad. Sci. USA 94:10239 ...
UV radiation can cause dimerization of RNA, which prevents transcription by RNA polymerase. If the viral genome follows a ... Since RNA-dependent RNA polymerase does not have an error-checking function, many mutations are made when the RNA is processed ... This is probably due to the advantage of having all RNA bound by N protein (since N binds hexamers of RNA). If RNA is left ... the RNA-dependent RNA polymerase pauses to release the new mRNA when it encounters an intergenic sequence. When the RNA ...
Heinrich Pette Institute
Dynamics of Viral Structures • Systems Arbovirology • Cell Biology of RNA Viruses Associated groups: • Quantitative Virology ... Viral Transformation • Virus Immunology • Virus-Host-Interaction • Viral Zoonoses - One Health • Integrative Virology Research ... at the Heinrich Pette Institute is to develop new approaches for improved diagnostic techniques and therapies for viral ...
The viral RNA is translated into a polyproteinss and then cleaved by viral and cellular proteases into the structural (C, prM, ... NS5 functions as the RNA-dependent RNA polymerase. NS1 is important in the viral replication process. NS2A interacts with NS3 ... A complementary negative sense RNA strand is formed via the RNA-dependent RNA polymerase (non-structural protein NS5) to create ... helps in viral assembly and recruits the viral RNA genome to membrane-bound replication complex. Secondary structures formed by ...
Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription is the method ... Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. ... The virus exits the host cell by tripartite non-tubule guided viral movement. Plant serve as the natural host. The virus is ... ". "Viral Zone". ExPASy. Retrieved 12 June 2015. Index of Viruses - Benyvirus (2006). In: ICTVdB - The Universal Virus Database ...
... has a single-stranded, linear, non-segmented RNA genome. The RNA genome is positively sensed meaning that it has ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the virus to host receptors, which ... Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription is the method ... "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. Malik YS, Bhat S, ...
Recombination occurs by a mechanism in which the viral RNA-dependent RNA polymerase switches templates during RNA synthesis ( ... Mechanism of RNA recombination in carmo- and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase ... RNA recombination occurs frequently during replication of carmoviruses, facilitating viral genome repair and promoting sequence ... Positive stranded rna virus transcription, using the premature termination model of subgenomic rna transcription is the method ...
Positive stranded RNA virus transcription is the method of transcription. The virus exits the host cell by tubule-guided viral ... Viral replication is cytoplasmic, and is lysogenic. Entry into the host cell is achieved by penetration into the host cell. ... "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) "Virus Taxonomy: 2019 Release". talk. ... The genome is segmented into two parts of linear, positive-sense, single-stranded RNA, 11000-12000 nucleotides in length, where ...
Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription is the method ... The virus exits the host cell by tubule-guided viral movement. Plants serve as the natural host. The virus is transmitted via a ... Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. ... "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) ICTV. "Virus Taxonomy: 2014 Release". ...
Viral replication is cytoplasmic. Replication follows the positive-strand RNA virus replication model. Positive-strand RNA ... The genome has one open reading frame which encodes the RNA-dependent RNA polymerase (RdRp). The genome is associated with the ... Mitovirus is a genus of positive-strand RNA viruses, in the family Mitoviridae. Fungi serve as natural hosts. There are five ... "Viral Zone". ExPASy. Retrieved 15 June 2015. Viralzone: Mitovirus ICTV. ...
Replication follows the double-stranded RNA virus replication model. Double-stranded RNA virus transcription is the method of ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment to host receptors, which mediates ... "Viral Zone". ExPASy. Retrieved 13 August 2015. CS1 maint: discouraged parameter (link) ICTV. "Virus Taxonomy: 2014 Release". ... The virus exits the host cell by monopartite non-tubule guided viral movement. Marine photosynthetic protists serve as the ...
Replication follows the double-stranded RNA virus replication model. Double-stranded RNA virus transcription is the method of ... Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. ... ". "Viral Zone". ExPASy. Retrieved 13 August 2015. CS1 maint: discouraged parameter (link) ICTV Online Report Partitiviridae ...
Positive stranded RNA virus transcription is the method of transcription. The virus exits the host cell by tubule-guided viral ... Its RNA suggests a replication mode like that of poleroviruses, whereas the coat protein sequence is closely related to that of ... Viral replication is cytoplasmic, and is lysogenic. Entry into the host cell is achieved by penetration into the host cell. ... CS1 maint: discouraged parameter (link) "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) ...
Replication follows the double-stranded RNA virus replication model. Double-stranded RNA virus transcription is the method of ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment to host receptors, which mediates ... "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) ICTV. "Virus Taxonomy: 2014 Release". ... The virus exits the host cell by monopartite non-tubule guided viral movement. Human, cattle, pig, mosquitoes (arthropod-borne ...
Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription is the method ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the virus to host receptors, which ... "Viral Zone". ExPASy. Retrieved 13 August 2015. CS1 maint: discouraged parameter (link) ICTV. "Virus Taxonomy: 2014 Release". ...
Replication follows the positive stranded RNA virus replication model. Positive stranded rna virus transcription is the method ... Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. ... "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. Viralzone: ...
Positive stranded RNA virus transcription, using the premature termination model of subgenomic RNA transcription is the method ... Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. Replication follows ... "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) ICTV. "Virus Taxonomy: 2014 Release". ... The virus exits the host cell by tubule-guided viral movement. Plants serve as the natural host. Transmission routes are ...
Positive stranded rna virus transcription is the method of transcription. Translation takes place by viral initiation, and ... These viruses can produce proteins directly from their RNA genome upon entering a cell; and therefore, does not require an RNA ... Genomes are linear and non-segmented, around 8.5-10.2kb in length, and has a VPg (genome linked viral protein) on the 5' end. ... The Cripavirus IRES is an RNA element that allows the virus to bind the ribosome and translate without a need for any ...
The viral genome encodes viral structural protein. Virions consist of 1 viral structural protein (major species), or 2 Viral ... Positive stranded RNA virus transcription is the method of transcription. Translation takes place by RNA termination- ... Viral structural protein: Capsid protein has a molar mass of 58000-60000 Da; is the coat protein. Capsid protein has a ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment to host receptors, which mediates ...
This rate is similar to that of other RNA viruses. Viral replication is cytoplasmic. Entry into the host cell is achieved by ... Lyssavirus genomes consist of a negative-sense, single-stranded RNA molecule that encodes five viral proteins: polymerase L, ... Replication follows the negative stranded RNA virus replication model. Negative stranded RNA virus transcription, using ... Lyssavirus (from the Greek λύσσα lyssa "rage, fury, rabies" and the Latin vīrus) is a genus of RNA viruses in the family ...
Viral DI RNAs with their small size and cis-acting elements are good templates both in vivo and in vitro on which to study RNA ... The replication process leaves a surplus of positive sense (+)RNA strands, and it is thought that not only does the viral RNA ... Downregulation of GAPDH reduced viral RNA accumulation, and eliminated the surplus of (+)RNA copies. Research has shown that ... Tomato bushy stunt virus Co-Opts the RNA-Binding Function of a Host Metabolic Enzyme for Viral Genomic RNA Synthesis. Cell Host ...
... es can have satellite RNAs encoding a toxin. Viral replication is cytoplasmic. Entry into the host cell is achieved by ... Replication follows the double-stranded RNA virus replication model. Double-stranded RNA virus transcription is the method of ... and an RNA-dependent RNA polymerase (RdRp). The 5' end of the positive strand of the dsRNA genome has no cap and is very ... Totivirus is a genus of double-stranded RNA viruses in the family Totiviridae. Fungi serve as natural hosts. The name of the ...
Inside the protein coat is the viral genome, which is bipartite, with the RNA strands referred to as RNA-1 and RNA-2. RNA-1 is ... and an RNA-dependent RNA polymerase domains. RNA-2 is 2,231 nucleotides in length and contains two ORFs, one at the 5' end and ... Positive stranded RNA virus transcription is the method of transcription. The virus exits the host cell by tubule-guided viral ... Infection has been shown to not occur if RNA-3 is either not present or is sufficiently damaged. Viral replication is ...
Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription is the method ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the virus to host receptors, which ... "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. Reuter G, Boros A, ... The viruses in this genus have single-stranded, linear non-segmented, positive-sense RNA genomes with lengths of approximately ...
Positive stranded rna virus transcription is the method of transcription. The virus exits the host cell by tubule-guided viral ... Viral replication is cytoplasmic, and is lysogenic. Entry into the host cell is achieved by penetration into the host cell. ... "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) ICTV. "Virus Taxonomy: 2014 Release". ... Replication follows the positive stranded RNA virus replication model. ...
Positive stranded RNA virus transcription is the method of transcription. Plants serve as the natural host. "Viral Zone". ... Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. Replication follows ... the positive stranded RNA virus replication model. ...
One such ORF encodes an RNA-dependent RNA polymerase. Several other ORFs are present at the 3' terminus and are expressed from ... Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. Replication follows ... The virus exits the host cell by tubule-guided viral movement. Plants serve as the natural host. The virus is transmitted via a ... Positive stranded RNA virus transcription is the method of transcription. Translation takes place by leaky scanning, -1 ...
The reason for this is that the RNA segments are not translated at the same rate. Viral particles begin to assemble in the ... Double-stranded RNA viruses Oncolytic virus Orphan virus "Viral Zone". ExPASy. Retrieved 15 June 2015. Guglielmi, KM; Johnson, ... This procedure is performed by newly synthesized RNAs. This event ensures that one each of the 11 different RNA segments is ... In early events, the selection process occurs so that the entry of the 11 different RNA segments go in the cell. ...
Xin-Cheng Qin et al.: A tick-borne segmented RNA virus contains genome segments derived from unsegmented viral ancestors, in: ... 3 RNA-Viren *3.1 Doppelsträngige RNA-Viren (dsRNA, double stranded RNA). *3.2 Einzelstrang-RNA-Viren mit negativer Polarität ( ... ss(−)RNA: negative single-stranded RNA). *3.3 Einzelstrang-RNA-Viren mit positiver Polarität (ss(+)RNA: positive single ... Einzelstrang-RNA-Viren mit negativer Polarität (ss(−)RNA: negative single-stranded RNA)[Bearbeiten , Quelltext bearbeiten]. ...
... meningitis and other viral haemorrhagic fevers may resemble EVD. Blood samples are tested for viral RNA, viral antibodies or ... viral RNA, or antibodies in blood. Differential diagnosis. Malaria, cholera, typhoid fever, meningitis, other viral ... detecting the viral RNA by polymerase chain reaction (PCR) and detecting proteins by enzyme-linked immunosorbent assay ( ... The viral RNA polymerase, encoded by the L gene, partially uncoats the nucleocapsid and transcribes the genes into positive- ...
Replication follows the positive stranded RNA virus replication model. Positive stranded rna virus transcription is the method ... "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. Viralzone: ...
Ribavirin is a prodrug which appears to interfere with viral replication by inhibiting RNA-dependent nucleic acid synthesis, ... Lassa fever, also known as Lassa hemorrhagic fever (LHF), is a type of viral hemorrhagic fever caused by the Lassa virus. ... Viral Hemorrhagic Fever Consortium Lassa fever Archived 4 April 2015 at the Wayback Machine. Page accessed April 6, 2016 ... Clinically, Lassa fever infections are difficult to distinguish from other viral hemorrhagic fevers such as Ebola and Marburg ...
SEPT5 - ويكيبيديا، الموسوعة الحرة
"Dopamine-dependent neurodegeneration in rats induced by viral vector-mediated overexpression of the parkin target protein, ...
... which serves as a molecular signal to stall the exonuclease and is the only viral requirement for subgenomic flavivirus RNA ( ... The sfRNAs are a result of incomplete degradation of the viral genome by the exonuclease and are important for viral ... "An RNA Pseudoknot Is Required for Production of Yellow Fever Virus Subgenomic RNA by the Host Nuclease XRN1". Journal of ... Other viral hemorrhagic fevers, such as Ebola virus, Lassa virus, Marburg virus, and Junin virus, must be excluded as the cause ...
... strand RNA genome is replicated through a double-stranded RNA intermediate that is formed using viral RDRP (RNA-Dependent RNA ... It has both icosahedral virus particles, viral RNA-dependent RNA polymerase and protease and viral replication proteins. But ... Also, the 3' end elements of viral RNA are significant and efficient for RNA replication of picornaviruses. The 3' end of ... The mRNA encodes RNA dependent RNA polymerase. This polymerase makes complementary minus strands of RNA, then uses them as ...
... were split off as a third domain because of the large differences in their ribosomal RNA structure. The particular RNA ... the impact of viral infection is higher on archaea than on bacteria and virus-induced lysis of archaea accounts for up to one- ... although there are many introns in their transfer RNA and ribosomal RNA genes, and introns may occur in a few protein- ... Werner F (September 2007). "Structure and function of archaeal RNA polymerases". Mol. Microbiol. 65 (6): 1395-404. doi:10.1111/ ...
... and viral genes. The TATA box was found in protein coding genes transcribed by RNA polymerase II. ... the TATA box is found at RNA polymerase II promoter regions, although some in vitro studies have demonstrated that RNA ... "RNA polymerase III accurately initiates transcription from RNA polymerase II promoters in vitro". The Journal of Biological ... TATA-binding protein (TBP) can be recruited in two ways, by SAGA, a cofactor for RNA polymerase II, or by TFIID. When ...
Positive stranded RNA virus transcription is the method of transcription. Translation takes place by leaky scanning, and RNA ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment to host receptors, which mediates ... Feline calicivirus "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. ... They are positive-sense, single stranded RNA which is non-segmented. There are currently seven species in this family, divided ...
Cold Spring Harbor Laboratory
RNA interference (RNAi) and small-RNA biology; DNA replication; RNA splicing; signal transduction; genome structure; non-coding ... A.D. Hershey and Martha Chase, "Independent Functions of Viral Protein and Nucleic Acid in Growth of Bacteriophage," J. General ... Adrian Krainer, studies RNA splicing and developed nusinersen for treatment of spinal muscular atrophy (SMA). ... Gregory Hannon (currently at CRUK Cambridge Institute), research on RNA interference, Member of the National Academy of ...
This is mostly shown for plant RNA viruses. Viroplasm is the location within the infected cell where viral replication and ... Viral evolution Viral replication Novoa, R. R.; Calderita, G.; Arranz, R.; Fontana, J.; Granzow, H.; Risco, C. (Feb 2005). " ... A viroplasm is an inclusion body in a cell where viral replication and assembly occurs. They may be thought of as viral ... Some of the membrane components are used for viral replication while some others will be modified to produce viral envelopes, ...
RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... viral pneumonia. DNA virus. *Epstein-Barr virus *EBV infection/ ... RNA virus. HCV Hepatocellular carcinoma. Splenic marginal zone lymphoma. HTLV-I Adult T-cell leukemia/lymphoma. ... RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ... The Spi-B factor was shown to be crucial in initiating viral replication in certain strains of transgenic mice. The protein ...
Soybean vein necrosis virus
The N protein contributes to viral replication, and coats the genomic RNA within the virion. Presently the soybean thrips ( ... The L segment is 9010 nt and encodes for the RNA-dependent RNA polymerase (RdRp). The M segment is 4955 nt and to encode for ... The genome of SVNV is a negative sense single stranded RNA virus (Group V) that has three segments (S, M, and L segments). ... Like other members of Bunyavirales, this virus is enveloped and has a negative sense single-stranded RNA (−ssRNA) genome ...
Index of HIV/AIDS-related articles
... viral burden - viral core - viral culture - viral envelope - viral load - viremia - viricide - virion - virology - virus - ... RNA) - ribosome - RNA - route of administration - RT-PCR - RTI - Ryan White C.A.R.E. act ... messenger RNA - metabolism - metastasis - MHC - microbes - microbicide - Microsporidiosis - mitochondria - mitochondrial ...
NPM1 - Wikipédia, a enciclopédia livre
RNA binding. • protein heterodimerization activity. • nucleic acid binding. • protein kinase binding. • core promoter binding. ... viral process. • DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest. • regulation of ... RNA polymerase II transcription coactivator activity. • transcription factor binding. • activating transcription factor binding ... 2006). «Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions.». Cell Res. 15 (11-12): 923-34 ...
... and use of endonuclease digestion of PCR-amplified RNA to demonstrate lack of mRNA expression from the second allele". American ...
Brain-derived neurotrophic factor
... of BDNF into the lateral ventricles doubled the population of newborn neurons in the adult rat olfactory bulb and viral ... RNA expression pattern. More reference expression data. Gene ontology. Molecular function. • receptor binding. • neurotrophin ...
This step will also make viral enzymes and capsid proteins (8). Viral RNA will be made in the nucleus. These pieces are then ... RNA: consists of a dimer RNA. It has a cap at the 5' end and a poly(A) tail at the 3' end. The RNA genome also has terminal ... Next, some of these RNA molecules are translated into viral proteins. For example, the gag gene is translated into molecules of ... The host cell then treats the viral DNA as part of its own genome, transcribing and translating the viral genes along with the ...
ವಂಶವಾಹಿ - ವಿಕಿಪೀಡಿಯ
೪೧.೦ ೪೧.೧ Eddy SR (December 2001). "Non-coding RNA genes and the modern RNA world". Nat. Rev. Genet. 2 (12): 919-29. doi: ... Hershey, AD; Chase, M (1952). "Independent functions of viral protein and nucleic acid in growth of bacteriophage". The Journal ... ೯೦.೦ ೯೦.೧ Claverie JM (September 2005). "Fewer genes, more noncoding RNA". Science 309 (5740): 1529-30. Bibcode:2005Sci... ... Domingo, E; Escarmís, C; Sevilla, N; Moya, A; Elena, SF; Quer, J; Novella, IS; Holland, JJ (June 1996). "Basic concepts in RNA ...
"Haemorrhagic fevers, Viral". World Health Organization. Archived from the original on 27 July 2019. Retrieved 19 June 2019.. ... but there is increasing evidence from DNA and RNA sequencing, that measles, smallpox, influenza, HIV, and diphtheria came to ... Other haemorrhagic fevers (Crimean-Congo haemorrhagic fever, Dengue fever, Lassa fever, Marburg viral haemorrhagic fever, Rift ...
RNA interference has been used to silence the expression of individual efflux transporters, either transiently or long-term. ... viral transfection research, and lipid transport. ... in Caco-2 cells using lentiviral vector-based short hairpin RNA ... "Investigation of the involvement of P-gp and MRP2 in the efflux of ximelagatran and its metabolites by using short hairpin RNA ...
List of atheists in science and technology
Norman Pirie FRS (1907-1997): British biochemist and virologist co-discoverer in 1936 of viral crystallization, an important ... milestone in understanding DNA and RNA.. *Henri Poincaré (1854-1912): French mathematician, theoretical physicist, ...
Replication follows the positive stranded RNA virus replication model. Positive stranded rna virus transcription is the method ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the virus to host receptors, which ... "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. Jones, MS.; Lukashov ... Translation takes place by -1 ribosomal frameshifting, viral initiation, and ribosomal skipping. The virus exits the host cell ...
Through the process of viral infection into hosts the three domains of life evolved. Another interesting proposal is ... Atkins JF, Gesteland RF, Cech T (2006). The RNA world: the nature of modern RNA suggests a prebiotic RNA world. Plainview, N.Y ... of RNAs with molecular properties predicted for RNAs of the RNA World constitutes an additional argument supporting the RNA ... Properties of RNA. The properties of RNA make the idea of the RNA world hypothesis conceptually plausible, though its ...
Amyotrophic lateral sclerosis
This also leads to decreased levels of RNA-binding protein in the nucleus, which may mean that their target RNA transcripts do ... In August 2014, a challenge went viral online, commonly known as the "ALS Ice Bucket Challenge". Contestants fill a bucket ... Other RNA metabolism genes associated with ALS include ANG, SETX, and MATR3. ... The zebrafish has transparent embryos that can be injected with DNA or RNA and has a lifespan of up to two years. Induced ...
Innate immune system
For example, the Influenza A virus produces NS1 protein, which can bind to host and viral RNA, interact with immune signaling ... which degrades viral RNA. Some viruses evade this by producing molecules which interfere with IFN production. ... RNA silencing mechanisms are also important in the plant systemic response, as they can block virus replication. The ... When host cells die, either by programmed cell death (also called apoptosis) or by cell injury due to a bacterial or viral ...
Alternately, trans-splicing of two non-functional RNA molecules may produce a single, functional RNA. Similarly, at the protein ... "Viral quasispecies evolution". Microbiology and Molecular Biology Reviews. 76 (2): 159-216. doi:10.1128/mmbr.05023-11. PMC ...
எயிட்சு - தமிழ் விக்கிப்பீடியா
2002). "Male viral load and heterosexual transmission of HIV-1 subtype E in northern Thailand". J. Acquir. Immune. Defic. Syndr ... 2005). "Mitochondrial DNA and retroviral RNA analyses of archival oral polio vaccine (OPV CHAT) materials: evidence of macaque ... Blankson JN, Persaud D, Siliciano RF (2002). "The challenge of viral reservoirs in HIV-1 infection". Annu. Rev. Med. 53: 557- ... Hurwitz BE, Klaus JR, Llabre MM, et al. (January 2007). "Suppression of human immunodeficiency virus type 1 viral load with ...
... detecting single stranded RNA. Following activation of the toll-like receptor, intracellular signaling cascades are initiated, ... these findings have not been examined in non-viral systems. ... of regulatory signaling during stress particularly after viral ... "Subversion of cellular autophagosomal machinery by RNA viruses". PLoS Biol. 3 (5): e156. doi:10.1371/journal.pbio.0030156. PMC ...
Link uncovered between viral RNA and human immune response
Bevilacqua »HIV-1 genome »Molecular Biology »PKR »RNA »RNA dimers »TAR »double-stranded RNAs »genetic material »human cells » ... Further reports about: , Bevilacqua , HIV-1 genome , Molecular Biology , PKR , RNA , RNA dimers , TAR , double-stranded RNAs , ... One way for this to happen is for the viral RNA to first form linked pairs called dimers. These RNA dimers then allow separate ... Link uncovered between viral RNA and human immune response. 06.08.2009. In its fight against an intruding virus, an enzyme in ...
quantitative viral RNA assay - Molecular Biology
Can AFM Reveal Global Viral RNA Structure?
... uncharacterized roles during the viral replication cycle. RNA structure with single-nucleotide resolution. In combination with ... we uncover several conserved RNA structures in the open reading frame of the viral genome. The… ... The genomes of RNA viruses often contain RNA structures that are crucial for translation and RNA replication and may play ... The genomes of RNA viruses often contain RNA structures that are crucial for translation and RNA replication and may play ...
Repositorio da Producao Cientifica e Intelectual da Unicamp: Gene Expression From Viral Rna Genomes
The strategies at the level of transcription presented here are synthesis of mRNA or subgenomic RNAs from viral RNA templates, ... This review is centered on the major strategies used by plant RNA viruses to produce the proteins required for virus ... leaky scanning of the initiation complex allows expression of more than one protein from the same RNA sequence. During ...
Viral Messenger RNA: Transcription, Processing, Splicing and by Joseph R. Nevins (auth.), Yechiel Becker, Julia Hadar (eds.) -...
Viral. , Viral Messenger RNA: Transcription, Processing, Splicing and by Joseph R. Nevins (auth.), Yechiel Becker, Julia Hadar ... Extra info for Viral Messenger RNA: Transcription, Processing, Splicing and Molecular Structure ... Read Online or Download Viral Messenger RNA: Transcription, Processing, Splicing and Molecular Structure PDF ... Viral Messenger RNA: Transcription, Processing, Splicing and by Joseph R. Nevins (auth.), Yechiel Becker, Julia Hadar (eds.). ...
Poliovirüs - Vikipedi
Virüs endositozla alınır, ve viral RNA serbest kalır (2). Translation of the viral RNA occurs by an IRES-mediated mechanism (3 ... The newly synthesized positive-sense RNA molecules can serve as templates for translation of more viral proteins (7) or can be ... The polyprotein is cleaved, yielding mature viral proteins (4). The positive-sense RNA serves as template for complementary ... Poliovirüs bir RNA genomu ve protein kapsülünden oluşmuştur. Genom yaklaşık 7500 nükleotid uzunluğunda olup, tek zincirlidir. ...
Viral RNA Replication Modes: Evolutionary and Dynamical Implications | SpringerLink
Sardanyés J. (2014) Viral RNA Replication Modes: Evolutionary and Dynamical Implications. In: Corral Á., Deluca A., Font-Clos F ... J. Sardanyés, F. Martínez, J.A. Daròs, S.F. Elena, Dynamics of alternative modes of RNA replication for positive-sense RNA ... L. Chao, C.U. Rang, L.E. Wong, Distribution of spontaneous mutants and inferences about the replication mode of the RNA ... F. Martínez, J. Sardanyés, J.A. Daròs, S.F. Elena, Dynamics of a plant RNA virus intracellular accumulation: stamping machine ...
Cas9-mediated targeting of viral RNA in eukaryotic cells | PNAS
To target the RNA of HCV, we engineered a small RNA, which we term an RNA-targeting guide RNA (rgRNA). The rgRNA is similar in ... Additionally, an rgRNA complementary to a portion of the 3′ UTR, necessary for replication of viral RNA, decreased viral ... 2009) RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell 139(5):945-956. ... Overall, programmable Cas9-mediated viral RNA targeting likely represents one of myriad potential applications of FnCas9 in RNA ...
Zika virus infection alters human and viral RNA | EurekAlert! Science News
These modifications -- chemical tags known as methyl groups -- influence viral replication and the human immune response. ... of California San Diego School of Medicine have discovered that Zika virus infection leads to modifications of both viral and ... the researchers removed the human enzymes responsible for adding methyl groups to viral RNA. Without m6A, the viral RNA was ... the cell modifies viral RNA with m6A as a means to get rid of the infection. RNA tagged with m6A is a beacon for human enzymes ...
Vaccines | Free Full-Text | Replicon RNA Viral Vectors as Vaccines
Replicon RNA vectors have also been subjected to clinical trials. Overall, immunization with self-replicating RNA viruses ... Immunization of mice, chicken, pigs and primates with virus-like particles, naked RNA or layered DNA/RNA plasmids has provided ... Administration of replicon RNA vectors has resulted in strong immune responses and generation of neutralizing antibodies in ... Moreover, recombinant particles and replicon RNAs have been encapsulated by liposomes to improve delivery and targeting. ...
Viral RNA detection by RIG-I-like receptors
Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) detect viral RNA as a non-self pattern in the cytoplasm and ... detect viral RNA as a non-self pattern in the cytoplasm and activate downstream signaling. Detection of viral RNA also ... Viral RNA detection by RIG-I-like receptors Curr Opin Immunol. 2015 Feb;32:48-53. doi: 10.1016/j.coi.2014.12.012. Epub 2015 Jan ... Among the three RLR family members RIG-I and melanoma differentiation-associated gene 5 (MDA5) recognize distinct viral RNA ...
Viral RNA Extraction Performance - Beckman Coulter
RNAdvance Viral and RNAdvance Viral XP, preform with documented data and downstream RT-PCR workflows. ... See how Beckman Coulters viral RNA extraction reagent kits, ... Viral RNA Extraction Performance Data. RNAdvance Viral and ... Product Information: Viral RNA Extraction Reagent Kits. RNAdvance Viral Reagent Kit - 768 Preps. Part number: C63510 ... Viral RNA extraction begins with lysis of the viral capsid from a variety of sample inputs, including saliva and nasopharyngeal ...
RNA viral community in human feces: prevalence of plant pathogenic viruses. - PubMed - NCBI
Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy ... and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the ... RNA viral community in human feces: prevalence of plant pathogenic viruses.. Zhang T1, Breitbart M, Lee WH, Run JQ, Wei CL, Soh ... C) RNA viruses were directly detected by RT-PCR from the total RNA of fecal sample 2: PMMV (lane 1), MCMV (lane 2), PBV, ...
Influenza viral RNA synthesis
However, the mRNAs are not complete copies of the viral (-) strand RNAs - they are missing sequen ... These molecules are then transported back to the cytoplasm, where they direct the synthesis of viral proteins. ... strand influenza viral RNAs enter the nucleus, they serve as templates for the synthesis of mRNAs. ... All influenza viral RNA synthesis is carried out by the viral RNA dependent RNA polymerase. The cap plus10-13 nucleotides are ...
PHASE Scientific Announces CE Mark Approval for the PHASIFY(TM) VIRAL RNA Extraction Kit
VIRAL RNA Extraction Kit. The CE marking confirms that PHASIFY™ VIRAL complies with the European In-Vitro Diagnostic Devices ... The PHASIFY™ VIRAL RNA Extraction Kit is designed to purify and concentrate viral RNA in patient viral transport media samples ... Utilizing PHASIFY™ VIRAL RNA extraction can result in 15-30-fold greater amount of viral RNA for standard RT-qPCR analyses, ... of the total viral RNA available for analyses. That suggests the current viral RNA extraction kits play a major role in ...
Viral reorganization of the secretory pathway generates distinct organelles for RNA replication. - PubMed - NCBI
A) Viral RNA and viral replication protein (3A, 3Dpol) subcellular distribution in early stages of CVB3 RNA replication.. (B) ... Viral RNA and viral replication protein (3A, 3Dpol) subcellular distribution at peak stages of CVB3 RNA replication. See also . ... J and K) PI4KIIIβ activity regulates viral RNA synthesis. Cell-free PV RNA translation (J) and synthesis (K) assays performed ... Viral reorganization of the secretory pathway generates distinct organelles for RNA replication.. Hsu NY1, Ilnytska O, Belov G ...
Advanced Cryo-EM reveals viral RNA replication complex structure in stunning detail
... scientists at the Morgridge Institute for Research have generated near atomic resolution images of a major viral protein ... In each positive-strand RNA virus, most of the viral genes are devoted to a single process: replicating the viral RNA genome. ... This prior work further showed that the key viral protein that induces the replication vesicles and copies the viral RNA ... The viral RNA replication protein that forms the crown is an extremely large, multi-domain, multi-functional protein, nearly ...
Viral vector-mediated RNA interference.
Abstract RNA interference (RNAi) is a powerful gene silencing mechanism that if properly harnessed has the potential to ... Delivery of inhibitory RNAs to target tissues needs to be safe, efficient, and for many diseases, long-lasting, in order to ... Viral vector systems, based on adeno-associated viruses and lentiviruses, are ideally suited to mediate RNAi because they can ... Abstract RNA interference (RNAi) is a powerful gene silencing mechanism that if properly harnessed has the potential to ...
PureLink Viral RNA/DNA Mini Kit - Thermo Fisher Scientific
The PureLink Viral Mini Kit is specifically designed to isolate high-q ... The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or ... The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or ... The PureLink® Viral RNA/DNA Mini Kit contains enough reagents for 50 reactions.. Kit Contents:. • 32 ml Viral Lysis buffer (L22 ...
Viral Messenger RNA | Springer for Research & Development
The nucleotide sequence of the gene from which messenger RNA mole- cules are transcribed is in a form that can be translated by ... The Interactions of Viral Proteins with Rous Sarcoma Virus RNA and Possible Control of Reverse Transcription, Translation and ... The Interaction between Viral Messenger RNA and Eukaryotic Initiation Factor 2, a Protein Involved in Translational Control ... This volume is devoted to current studies in the field of cellular and viral messenger RNA. The studies presented provide an ...
Microbial Transcriptomics | Bacterial and viral RNA-Seq
RNA-Seq) offers an unbiased approach for analyzing and quantifying bacterial, viral, and other microbial transcripts. ... RNA-Seq for Microbial Transcript Analysis. Bacterial, viral, and other microbial RNA-Seq experiments enable annotation and ... Note: If using the TruSeq RNA library prep kit, you will NOT need to extract RNA and transcribe to cDNA. If you use an RNA ... Next-generation RNA sequencing (RNA-Seq) of bacteria, viruses, and other microbes has become a standard method for analyzing ...
Maxwell® RSC Viral Total Nucleic Acid Purification Kit | Viral RNA | Viral DNA
RNA and DNA) from serum, plasma or other samples using the Maxwell RSC Instrument. ... The Maxwell RSC Viral Total Nucleic Acid Purification Kit is designed for automated extraction of viral total nucleic acid ( ... Automated Purification of Viral Total Nucleic Acid. *Extracts viral total nucleic acid (RNA and DNA) from serum, plasma and ... RSC Viral Total Nucleic Acid Purification Kit is designed for automated extraction of viral total nucleic acid (RNA and DNA) ...
A Viral RNA Structural Element Alters Host Recognition of Nonself RNA | Science
A Viral RNA Structural Element Alters Host Recognition of Nonself RNA Message Subject. (Your Name) has forwarded a page to you ... A Viral RNA Structural Element Alters Host Recognition of Nonself RNA. By Jennifer L. Hyde, Christina L. Gardner, Taishi Kimura ... A Viral RNA Structural Element Alters Host Recognition of Nonself RNA. By Jennifer L. Hyde, Christina L. Gardner, Taishi Kimura ... As an example, 2-O methylation of the 5′ cap of viral RNA subverts mammalian antiviral responses by evading restriction of ...
Thermo Scientific MagJET Viral DNA and RNA Kit For 96 preparations:Life
Thermo Scientific MagJET Viral DNA and RNA Kit For 96 preparations Life Sciences:Biochemicals and Reagents:DNA Extraction and ... Thermo Scientific MagJET Viral DNA and RNA Kit Purifies viral nucleic acids from human and animal samples, including plasma, ... Quality Control:The MagJET Viral DNA and RNA Kit has been tested by isolating DNA and RNA from 200 µL of human plasma spiked ... The Thermo Scientific MagJET Viral DNA and RNA Kit is designed for fast and efficient purification of viral nucleic acids from ...
Single POC test detecting HIV antibodies, viral RNA in development
Quick-DNA/RNA Viral Kit
... saliva and any other biological samples stored in DNA/RNA Shield. DNA/RNA Shield ensures nucleic acid stability during sample ... The Quick-DNA/RNA Viral Kit is a fast viral DNA/RNA purification kit for viral DNA and RNA from plasma, serum, cell culture ... The Quick-DNA/RNA Viral Kit is a fast viral DNA/RNA purification kit for viral DNA and RNA from plasma, serum, cell culture ... Viral DNA/RNA is bound to the column, washed, and eluted. The isolated high-quality viral DNA/RNA are ready for all downstream ...
Reply to Finol: Viral small RNA from Dengue virus and its regulatory role in different serotypes | PNAS
Reply to Finol: Viral small RNA from Dengue virus and its regulatory role in different serotypes. Mazhar Hussain and Sassan ... Reply to Finol: Viral small RNA from Dengue virus and its regulatory role in different serotypes ... Are viral small RNA regulating Dengue virus replication beyond serotype 2? - July 14, 2014 ...
New study shows promise in using RNA nanotechnology to treat cancers and viral infections | EurekAlert! Science News
A new study by University of Kentucky researchers shows promise for developing ultrastable RNA nanoparticles that may help ... treat cancer and viral infections by regulating cell function and binding to cancers without harming surrounding tissue. ... Their RNA nanoparticles can include small interfering RNA for silencing genes, micro-RNA for regulating gene expression, ... New study shows promise in using RNA nanotechnology to treat cancers and viral infections. University of Kentucky ...
Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA...
... via transcriptional slippage of viral RNA-dependent RNA polymerase (RdRp). We herein show that clover yellow vein virus (ClYVV ... Deep sequencing of ClYVV RNA from infected plants endorses the slippage by viral RdRp. Our findings unveil a virus strategy ... RNA viruses use various strategies to condense their genetic information into small genomes. Potyviruses not only use the ... The RNA-Dependent RNA Polymerase NIb of Potyviruses Plays Multifunctional, Contrasting Roles during Viral Infection *Wentao ...
Nuclear Export of Viral RNAs by Joachim Hauber, P. K. Vogt | Waterstones
Buy Nuclear Export of Viral RNAs by Joachim Hauber, P. K. Vogt from Waterstones today! Click and Collect from your local ... Nuclear Export of Viral RNAs - Current Topics in Microbiology and Immunology 259 (Paperback). Joachim Hauber (editor), P. K. ... CRM I appears to be involved in the nucleocytoplasmic translocation of the vast majority of viral and cellular proteins that ...
Viral RNA/DNA Purification | Thermo Fisher Scientific - US
Viral RNA and DNA capture, purification and qRT-PCR and qPCR analyses; featuring PureLink™, RNA UltraSense™, & Dynabeads® ... Specific Capture of Viral RNA/DNA Target a specific viral RNA or DNA sequence and capture only that specific nucleic acid ... Dynabeads® SILANE Viral RNA/DNA Sensitive and highly consistent isolation of viral RNA and DNA from cell-free samples. These ... Viral RNA/DNA purification Both spin-column and 96-well plate kits are designed for fast and easy isolation of viral RNA or DNA ...
British Library EThOS: Viral vector-mediated RNA interference in the retina
RNA interference (RNAi) is a highly conserved post-transcriptional gene silencing process triggered by double-stranded RNA ( ... The aim of this thesis is to evaluate the efficacy of viral vector-mediated RNAi in the retina using recombinant adeno- ... The efficient gene silencing achieved by these short hairpin RNA (shRNA) molecules and the cumulative understanding of the RNAi ... A detailed assessment of the utility and extend of RNAi in the retina using different viral vectors and hairpin designs is ...
Structure Cluster - 1L2X: Atomic Resolution Crystal Structure of a Viral RNA Pseudoknot 3D Similarity Report Page
ReplicationVirusesProteinsGenomeRibonucleicSynthesisSubgenomicPoliovirusCopiesRecombinantDetectionMechanismReplicationGenomesGenomicSingle-strandedAbstract RNACellularGene expressionAssaysIntracellularSerumNucleic acidMutationsDsRNAGenesMembranesSiRNAVector-mediated RNA interferenceLysis BufferBiologicalInterferencePurification of high-qualityInhibition of viralReplicateIsolation of viralHigh-quality viralSARS-CoV-2 RNAMoleculeInfectionsAmount of viralDetectableCapsidSmall inteFunctionalImmuneInhibitBindsSpeciesTherapeuticKineticsStructuresDesigned to purifyMutantsGeneticSuppressionMRNAPathogenicEndogenousDownstreamInfectiousEukaryoticCellsAnalyses
- The genomes of RNA viruses often contain RNA structures that are crucial for translation and RNA replication and may play additional, uncharacterized roles during the viral replication cycle. (wordpress.com)
- These structures are crucial for translation and replication of the viral genome and may play additional roles in other processes, such as genome packaging and modulation of the host antiviral response. (wordpress.com)
- The genomes of RNA viruses, such as poliovirus (PV), often contain complex RNA secondary and tertiary structures. (wordpress.com)
- The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts. (epo.org)
- In combination with orthogonal evolutionary analyses, we uncover several conserved RNA structures in the open reading frame of the viral genome. (wordpress.com)
- We identified many regions of the poliovirus genome where SHAPE data indicate that nucleotide flexibility is constrained, suggesting that the RNA is folded into secondary and/or tertiary structures. (wordpress.com)
- The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. (epo.org)
- We have used SHAPE to interrogate authentic full-length poliovirus genomic RNA extracted, in a gentle and non-denaturing manner, from purified virions. (wordpress.com)
- Kısa genom ve basit kompozisyon olması sebebiyle sadece RNA ve-poliovirus, bu kapsüller olmayan bir zarflı Ikosahedral protein tabakası nedeniyle yaygın basit anlamlı bir virüs olarak kabul edilir. (wikipedia.org)
- The recombinant viral nucleic acid provides the particular advantage that it systematically transcribes the second nucleic acid in the host. (epo.org)
- F. Martínez, J. Sardanyés, J.A. Daròs, S.F. Elena, Dynamics of a plant RNA virus intracellular accumulation: stamping machine versus geometric replication. (springer.com)
- J. Sardanyés, S.F. Elena, Quasispecies spatial models for RNA viruses with different replication modes and infection strategies. (springer.com)
- J. Sardanyés, F. Martínez, J.A. Daròs, S.F. Elena, Dynamics of alternative modes of RNA replication for positive-sense RNA viruses. (springer.com)
- J. Sardanyés, R.V. Solé, S.F. Elena, Replication mode and landscape topology differentially affect RNA virus mutational load and robustness. (springer.com)
- These modifications -- chemical tags known as methyl groups -- influence viral replication and the human immune response. (eurekalert.org)
- Without m6A, the viral RNA was more stable and viral replication increased, as compared to human cells with normal methylation enzymes. (eurekalert.org)
- Viral reorganization of the secretory pathway generates distinct organelles for RNA replication. (nih.gov)
- Many RNA viruses remodel intracellular membranes to generate specialized sites for RNA replication. (nih.gov)
- Here we show how RNA viruses can manipulate multiple components of the cellular secretory pathway to generate organelles specialized for replication that are distinct in protein and lipid composition from the host cell. (nih.gov)
- These findings reveal how RNA viruses can selectively exploit specific elements of the host to form specialized organelles where cellular phosphoinositide lipids are key to regulating viral RNA replication. (nih.gov)
- A) Viral RNA and viral replication protein (3A, 3D pol ) subcellular distribution in early stages of CVB3 RNA replication. (nih.gov)
- B) Viral RNA and viral replication protein (3A, 3D pol ) subcellular distribution at peak stages of CVB3 RNA replication. (nih.gov)
- F) Arf1 and GBF1 facilitate viral RNA replication. (nih.gov)
- G) Functional ER exit sites facilitate viral RNA replication. (nih.gov)
- Advanced cryo-EM imaging reveals high-resolution side and top views of the viral RNA replication "crown" complex structure. (newswise.com)
- Using an advanced technique called cryoelectron microscope (cryo-EM) tomography, Ahlquist and his team built upon their previous work , which first revealed the existence of this crown-like viral RNA replication complex. (newswise.com)
- Given this massive investment of resources, viral RNA genome replication is arguably one of the most important processes in infection, and It is already a major target for virus control," Ahlquist says. (newswise.com)
- Within an infected cell, viral RNA replication occurs at modified cellular membranes, often in association with spherules, virus-induced vesicles approximately 50-100 nanometers in size. (newswise.com)
- Ahlquist and his team previously showed that in each such genome replication complex, a copy of the viral RNA genome or chromosome is protected inside the spherule vesicle to function as a replication template. (newswise.com)
- The replication complex repeatedly copies this archival viral RNA chromosome to produce new progeny genomes that are released through a membranous neck on the vesicle into the cytoplasm, where they are incorporated as the payload of new infectious virions. (newswise.com)
- This prior work further showed that the key viral protein that induces the replication vesicles and copies the viral RNA resides in a striking ring or crown structure that sits atop the cytoplasmic side of the spherule neck that connects with the cytoplasm. (newswise.com)
- The new higher resolution cryo-EM images and complementary results show that the crown is composed of twelve copies of the key viral RNA replication protein arranged like staves in a barrel. (newswise.com)
- The viral RNA replication protein that forms the crown is an extremely large, multi-domain, multi-functional protein, nearly 1000 amino acids in size. (newswise.com)
- Using an approach that combined a genetically engineered, site-specific tag with labeling by nanoscale gold particles visible in cryo-EM, the researchers found that the C-terminal polymerase end of the viral RNA replication protein is positioned at the apex of the crown, leaving the N-terminal capping domain at the bottom of the structure to interact with the membrane. (newswise.com)
- Are viral small RNA regulating Dengue virus replication beyond serotype 2? (pnas.org)
- Therefore, in order to understand the functionality of viral lncRNA, Sztuba-Solinska and her team have been addressing the relationship between the various aspects of lncRNA conformation, i.e. secondary structure, tertiary interactions, epitranscriptomic modifications, and lncRNA functionality during viral replication and pathogenesis. (auburn.edu)
- CPs have been shown to regulate the infection processes of RNA viruses, including RNA replication and gene expression. (dovepress.com)
- Accumulating evidence suggests that phosphorylation of viral CPs is involved in the regulation of the viral infection process from enabling virion disassembly to regulation of viral protein synthesis and replication. (dovepress.com)
- Flaviviruses are known to cause remodeling of intracellular membranes into small cavities, where replication of the viral RNA takes place. (diva-portal.org)
- Nonstructural (NS) proteins are not part of the virus coat and are thought to participate in the formation of these viral replication compartments (RCs). (diva-portal.org)
- Here, we used tick-borne encephalitis virus (TBEV) as a model for the flaviviruses and developed a stable human cell line in which the expression of NS proteins can be induced without viral RNA replication. (diva-portal.org)
- We propose that the NS proteins drive the remodeling of ER membranes and that viral RNA, RNA replication, viral polymerase, and TBEV structural proteins are not required. (diva-portal.org)
- Similar to other flaviviruses, TBEV exploits intracellular membranes to build RCs for viral replication. (diva-portal.org)
- To study how TBEV induces membrane remodeling, we developed an inducible stable cell system expressing the TBEV NS polyprotein in the absence of viral RNA replication. (diva-portal.org)
- The multiscale model of hepatitis C virus (HCV) dynamics, which includes intracellular viral RNA (vRNA) replication, has been formulated in recent years in order to provide a new conceptual framework for understanding the mechanism of action of a variety of agents for the treatment of HCV. (frontiersin.org)
- biphasic model [ 20 ] that was introduced in 1998 and treated the infected cell as a "black box," producing virions but without any consideration of the intracellular viral RNA replication and degradation within the infected cell [ 26 , 27 , 42 ]. (frontiersin.org)
- Is the Subject Area "Viral replication" applicable to this article? (plos.org)
- All killer virus-infected cells as well as nearly all nonkillers harbor the replication-competent L-A dsRNA viral genome, which functions as a helper virus for satellites, such as M dsRNA. (genetics.org)
- Early growth response-1 facilitates enterovirus 71 replication by direct binding to the viral genome RNA. (sigmaaldrich.com)
- We further reveal that EV71-activated EGR1, in turn, regulates the internal ribosomal entry site (IRES) of EV71 to enhance viral replication. (sigmaaldrich.com)
- Moreover, EGR1 protein co-localizes with EV71 RNA in the cytoplasm of infected cells to facilitate viral replication. (sigmaaldrich.com)
- Our results reveal an important new role of EGR1 in viral infection, provide new insight into the novel mechanism underlying the regulation of EV71 replication, and suggest a potential application of EGR1 in the control of EV71 infection. (sigmaaldrich.com)
- The viral DI RNA associates with multiple viral proteins during replication, and is therefore expected to form heterogeneous RNA-protein complexes. (illinois.edu)
- In addition, the antiviral 'arsenal' of the host cells includes specialised RNA-binding proteins that recognise viral RNA and intermediaries of replication. (bmglabtech.com)
- For RNA viral genomes, the information encoded in the sequence extends well-beyond their protein coding role to the role of intra-sequence base pairing in viral packaging, replication, and gene expression. (umn.edu)
- This TLR-independent induction of IFN-αβ after SV infection is replication dependent and mediated by the RNA helicase, retinoic acid-inducible gene-I (RIG-I) and not the related family member, melanoma differentiation-associated gene 5. (jimmunol.org)
- Successful host defense against virus infection relies on the rapid production of IFN-αβ and the subsequent transcription of hundreds of so-called IFN-stimulated genes (ISGs), 4 the products of which lead to a cellular antiviral state and prevent viral replication. (jimmunol.org)
- TLRs are one class of PRRs capable of detecting viral particles or products of viral replication. (jimmunol.org)
- Many negative-strand RNA viruses replicate in the cytoplasm and are unlikely to expose structures produced during viral replication to TLRs (e.g., dsRNA, a product of viral replication). (jimmunol.org)
- Accordingly, TLR-mediated sensing of viruses by endosomally localized TLRs occurs without the requirement for viral replication. (jimmunol.org)
- Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. (sigmaaldrich.com)
- NS5A is critically involved in viral RNA replication that takes place at newly formed membranes within the endoplasmic reticulum (membranous web) and assists viral assembly in the close vicinity of lipid droplets (LDs). (sigmaaldrich.com)
- A similar reduction was observed when TIP47 was knocked down in cells harboring an autonomously replicating HCV RNA (subgenomic replicon), indicating that TIP47 is required for efficient HCV RNA replication. (sigmaaldrich.com)
- A single point mutation (W9A) in NS5A that disrupts the interaction with TIP47 but preserves proper subcellular localization severely decreased HCV RNA replication. (sigmaaldrich.com)
- While most mutations are known to originate in replication errors, difficulties of capturing the underlying dynamics have left the mechanochemical basis of viral mutagenesis unresolved. (tudelft.nl)
- To determine whether persistent RNA was capable of resuming unrestricted replication in the absence of the continuous presence of antiviral antibodies, viral titers were measured in the brains of scid mice at 1, 2, 3, and 6 months after antibody treatment. (asm.org)
- Replication of infectious virus isolated from scid mouse brain could be restricted by repeat treatment with immune serum, indicating that viral reactivation is not due to antibody-escape mutations. (asm.org)
- Encapsidation of viral RNA in Picornavirales: studies on cowpea mosaic virus demonstrate dependence on viral replication. (jic.ac.uk)
- RNA-free virus-like particles (eVLPs) can be generated by transiently co-expressing the RNA-2-encoded coat protein precursor (VP60) with the RNA-1-encoded 24K protease, in the absence of the replication machinery (Saunders et al. (jic.ac.uk)
- We have made use of the ability to produce assembled capsids of CPMV in the absence of replication to examine the putative linkage between RNA replication and packaging in the Picornavirales We have created a series of mutant RNA-1 and RNA-2 molecules and have assessed the effect of the mutations on both the replication and packaging of the viral RNAs. (jic.ac.uk)
- We demonstrate that mutations that affect replication have a concomitant impact on encapsidation, and that RNA-1 -mediated replication is required for encapsidation of both RNA-1 and RNA-2. (jic.ac.uk)
- This close coupling between replication and encapsidation provides a means for the specific packaging of viral RNAs. (jic.ac.uk)
- Moreover, we demonstrate that this feature of CPMV can be used to specifically encapsidate custom RNA by placing a sequence of choice between the RNA-2 sequences required for replication.IMPORTANCE The mechanism whereby members of the order Picornavirales specifically package their genomic RNAs is poorly understood. (jic.ac.uk)
- Research with monopartite members of the order, such as poliovirus, have indicated that packaging is linked to replication, though the presence of 'packaging signals' along the length of the viral RNA has also been suggested. (jic.ac.uk)
- Thanks to the bipartite nature of the CPMV genome which allows the manipulation of RNA-1 without modifying RNA-2, we show here that this specificity is due to a functional linking between the two processes of viral replication and encapsidation. (jic.ac.uk)
- These compounds are precursors to inhibitors of RNA-dependent RNA viral replication and are useful for the treatment of RNA-dependent RNA viral infection. (patents.com)
- Also disclosed are methods of inhibiting RNA-dependent RNA polymerase, inhibiting RNA-dependent RNA viral replication, and/or treating RNA-dependent RNA viral infection with the nucleoside aryl phosphoramidates of the present invention. (patents.com)
- Our findings suggest that AUF1 restriction of poliovirus and CVB3 replication uses a common mechanism through the viral IRES, which is distinct from the canonical role that AUF1 plays in regulated mRNA decay in uninfected host cells. (asm.org)
- As part of the alteration of the host cell landscape to promote virus replication, picornaviruses modify lipid metabolism and reorganize membrane architecture to form replication complexes, downregulate host cell transcription and translation to redirect cellular resources to favor viral replication, and disrupt nucleocytoplasmic trafficking to relocate nuclear proteins required for replication into the cytoplasm ( 1 - 4 ). (asm.org)
- Triggered by double-stranded RNAs that form during viral RNA synthesis, the innate immune system is the best-described process that restricts virus replication, and as a result, picornaviruses have evolved numerous strategies to counteract this response ( 5 , 6 ). (asm.org)
- However, the dependence on cellular proteins to promote each step of the virus replication cycle provides opportunities for viral interactions with cellular restriction factors. (asm.org)
- These results suggest that UV-LEDs inhibit host cell replication and transcription of viral RNA. (flutrackers.com)
- Formation of viral particles and packaging of genomic retroviral RNA into these particles are important steps in the late phase of the viral replication cycle. (bio-protocol.org)
- It specifically provides the roles of viral proteins and RNA sequences in RNA replication, and describes the kinetics and products of RNA replication in infected cells. (asmscience.org)
- Next, it explains the sites and compositions of viral replication complexes (RCs) in infected cells. (asmscience.org)
- In 2017, a team led by Paul Ahlquist, director of the John W. and Jeanne M. Rowe Center for Research in Virology at the Morgridge Institute and professor of oncology and molecular virology at UW-Madison, first revealed the existence of this crown-like viral RNA replication complex. (technologynetworks.com)
- Within an infected cell, viral RNA replication happens at cellular membranes, often upon the formation of virus-induced compartments known as spherules. (technologynetworks.com)
- The replication complex repeatedly copies this archival viral RNA to produce new genomes that are released from the spherule through a membranous neck and then incorporated as the payload of new infectious virions. (technologynetworks.com)
- The team's prior work also showed that the crown structure, which sits atop the spherule neck, contains a key viral protein that creates the replication complex and copies the viral RNA. (technologynetworks.com)
- The new higher-resolution cryo-EM images and complementary results show that the crown is composed of 12 copies of the key viral RNA replication protein, arranged like staves in a barrel. (technologynetworks.com)
- For instance, they found that the polymerase domain that actually synthesizes new RNA is positioned at the apex of the crown, providing clues about the early steps in the replication process in which the starting viral RNA template is recruited into the complex and the spherule is formed. (technologynetworks.com)
- We hope to continue to improve the RNA replication complex crown structure to provide additional important refinements in the future," Ahlquist says. (technologynetworks.com)
- Mutant 5NC-11, having a 4-base insertion at nucleotide 70, was dramatically deficient in RNA synthesis, suggesting that the far 5' end of the genome is primarily involved in one or more steps of RNA replication. (caltech.edu)
- Hepatitis B virus (HBV) RNA in serum is a novel biomarker for intrahepatic HBV replication and treatment response. (eur.nl)
- Following viral DNA replication, late viral genes, which mainly encode structural proteins, start to transcribe and ultimately lead to the assembly and release of infectious particles. (prolekare.cz)
- Even though it has been demonstrated that the NV gene is not necessary for viral replication, it is highly essential for viral pathogenesis. (wikipedia.org)
- We propose that this protein-mediated RNA selection mechanism may underpin the high fidelity assembly of multi-segmented RNA genomes in Reoviridae. (uni-muenchen.de)
- These mathematical results yield insights into the interaction of local and global constraints in RNA secondary structures, and suggest new directions in understanding the folding of RNA viral genomes. (umn.edu)
- To explore whether Sindbis virus genomes persist in mouse brain after the clearance of infectious virus, we used reverse transcriptase-polymerase chain reaction amplification methods to detect Sindbis virus RNA in brain samples from immunocompetent BALB/c and antibody-treated immunodeficient scid/CB17 mice. (asm.org)
- In this study, we describe sequence-independent amplification for samples containing ultra-low amounts of viral RNA coupled with Illumina sequencing and de novo assembly optimized for viral genomes. (pubmedcentralcanada.ca)
- The methods presented here are scalable to large numbers of samples and capable of generating full or near full length viral genomes from clone and clinical samples with low amounts of viral RNA, without prior sequence information and in the presence of substantial host contamination. (pubmedcentralcanada.ca)
- Massively parallel sequencing allows for rapid and low-cost deep sequencing of viral genomes and provides an opportunity to gain greater insight into viral evolution, fitness, emergence and transmission. (pubmedcentralcanada.ca)
- The bias of A-rich codons in HIV-1 pol is thought to be a record of hypermutations in viral genomes that lack biological functions. (pubmedcentralcanada.ca)
- Using codon modifications to reduce the amount of A-rich sequences within HIV-1 genomes, we have reduced the flexibility of RNA sequences in pol to analyze the functional significance of these A-rich 'structurally poor' RNA elements in HIV-1 pol . (pubmedcentralcanada.ca)
- However, alphaviruses replicate efficiently in cells expressing Ifit1 even though their genomic RNA has a 5′ cap lacking 2'-O methylation. (sciencemag.org)
- Specifically, the highly replicative sendai virus sub-genomic defective interfering (DI) RNA is targeted. (illinois.edu)
- Instead of focusing on changes in host gene expression at sites near viral genomic integration sites, Lau and colleagues tested whether HBV insertion events in HCC cell lines could lead to the generation of viral-human chimeric transcripts or activate the transcription of silent repetitive sequences. (aacrjournals.org)
- Our data showed that codon modification of HIV-1 sequences led to a suppression of virus infectivity by 5-100-fold, and this defect does not correlate with, viral entry, viral protein expression levels, viral protein profiles or virion packaging of genomic RNA. (pubmedcentralcanada.ca)
- Flavivirus is a positive-sense, single-stranded RNA viral genus, with members causing severe diseases in humans such as tick-borne encephalitis, yellow fever, and dengue fever. (diva-portal.org)
- Oligonucleotides were synthesized according to the amino acid sequence information, and used as probes for Northern blot analyses of four single-stranded RNA species (segments 1-4) and four double-stranded RNA species of RSV. (eurekamag.com)
- VHSV is a negative-sense single-stranded RNA virus of the order Mononegavirales, family Rhabdoviridae, and genus Novirhabdovirus. (wikipedia.org)
- The nucleotide sequence of the gene from which messenger RNA mole- cules are transcribed is in a form that can be translated by cellular ribosomes into the amino acid sequence of a particular polypeptide, the product of the gene. (springer.com)
- This volume is devoted to current studies in the field of cellular and viral messenger RNA. (springer.com)
- This book should be of interest to all students of cellular and viral genes and scientists in the field. (springer.com)
- The Quick -DNA/RNA Viral Kit is a fast viral DNA/RNA purification kit for viral DNA and RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield. (zymoresearch.com)
- The identified protein regions involved in packaging viral RNAs bind random cellular RNA with high affinity and standard methods of identifying RNA-protein interactions such as gel shift mobility assays will be unable to discriminate between specific and unspecific binding. (uwaterloo.ca)
- Protein and RNA molecules interact with multiple protein partners to perform essential cellular processes such as post-transcriptional regulation of mRNA. (illinois.edu)
- Using a similar principle, the objective of this study is to extend the SiMPull assay to isolate and study single cellular RNA-protein complexes. (illinois.edu)
- Therefore, this study provides evidence for the applicability of SiMPull to isolate and study single cellular RNA-protein interactions. (illinois.edu)
- The efficiency of the incorporation of viral or cellular RNAs into viral particles can be studied using a quantitative Reverse Transcriptase-PCR (RT-qPCR)-based approach. (bio-protocol.org)
- There are now many examples of the use of viral vector-mediated RNAi to inhibit gene expression in animal models of disease, and in many cases proof-of-principle has been demonstrated. (omicsonline.org)
- Their RNA nanoparticles can include small interfering RNA for silencing genes, micro-RNA for regulating gene expression, aptamer for targeting cancer cells, or a ribozyme that can catalyze chemical reactions. (eurekalert.org)
- Rev-RRE interaction regulates viral gene expression by controlling the export of spliced and unspliced mRNAs into the cytoplasm. (uwaterloo.ca)
- Thus, the [KIL-d] effect on viral gene expression is epigenetic in nature. (genetics.org)
- In a mouse model of Sindbis virus encephalitis, we have previously shown that clearance of infectious virus is mediated by antibody-induced restriction of viral gene expression rather than by cytotoxic destruction of virally infected cells. (asm.org)
- Here we show that HCMV pUL79, one of these factors, interacts with RNAP II as well as other viral factors involved in late gene expression. (prolekare.cz)
- Commercially available HIV-1 RNA assays do not detect HIV-2 viral load. (nih.gov)
- Comparison of the results of five kinds of assays of HCV antibodies and HCV RNA. (annals.org)
- Discrepancies in viral load (VL) measurements obtained in different plasma collection tubes have underscored the importance of specimen collection and handling in the determination of accurate results in HIV viral load assays. (asm.org)
- Elevated HIV-1 viral loads in plasma specimens collected and frozen in PPTs and quantified in the standard and ultrasensitive Roche COBAS Amplicor HIV-1 Monitor assays ( 2 , 4 , 13 ) have led investigators to believe that it may have an impact on therapeutic management of HIV-infected patients ( 1 , 8 ). (asm.org)
- The large differences are due to different blip definitions and viral load assays. (biomedcentral.com)
- Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. (uzh.ch)
- Correlation and concordance between the viral load assays were lower at a low pVL. (uzh.ch)
- Our investigation clearly shows that both proviral DNA and intracellular RNA are amplified simultaneously in the COBAS Amplicor HIV-1 Monitor assay and that proviral DNA contributes to the elevated VL in plasma frozen in PPTs. (asm.org)
- Journal Article] Foreign RNA induces the degradation of mitochondrial antiviral signaling protein (MAVS)the role of intracellular antiviral factors. (nii.ac.jp)
- UV-LED irradiations did not alter hemagglutination titer, but decreased accumulation of intracellular total viral RNA in infected MDCK cells was observed. (flutrackers.com)
- Additionally, UV-LED irradiations suppressed the accumulation of intracellular mRNA (messenger RNA), vRNA (viral RNA), and cRNA (complementary RNA), as measured by strand-specific RT-PCR. (flutrackers.com)
- The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. (thermofisher.com)
- Purifies viral nucleic acids from human and animal samples, including plasma, serum, saliva, and urine, using magnetic bead technology amenable to high-throughput operations. (fishersci.com)
- The Thermo Scientific MagJET Viral DNA and RNA Kit is designed for fast and efficient purification of viral nucleic acids from various human and animal liquid samples such as plasma, serum, saliva and urine, as well as from nasal, buccal and urogenital swabs and blood. (fishersci.com)
- Quick recovery of viral DNA/RNA from plasma, serum and other samples. (zymoresearch.com)
- and chemically stable, which makes the nanoparticles resistant to RNase (an enzyme, which cleaves RNA) digestion in the blood serum. (eurekalert.org)
- Both spin-column and 96-well plate kits are designed for fast and easy isolation of viral RNA or DNA from cell-free samples such as serum, plasma, cerebrospinal fluid, and cell culture supernatant. (thermofisher.com)
- Most of these appear to represent false-positive results because HCV RNA is usually absent from the serum. (annals.org)
- Viral reactivation was seen in scid mice treated with hyperimmune serum or a low dose of monoclonal antibody to the E2 envelope glycoprotein, but not in mice treated with a high dose of monoclonal antibody to E2. (asm.org)
- The GRS Viral DNA/RNA Purification Kit provides an efficient and fast method for the purification of high quality viral DNA and RNA from cell-free media (e.g. from serum, body fluids, and the supernatant of viral infected cell cultures). (bioquote.com)
- The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral DNA and viral RNA from cell-free fluids such as plasma, serum, urine and cell culture supernatant. (tebu-bio.com)
- Mag-Bind® Viral DNA/RNA Kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from whole blood, serum, plasma, saliva and other body fluids. (omegabiotek.com)
- For its proper use, it is essential to identify factors influencing serum HBV RNA level. (eur.nl)
- We explored the association of serum levels of HBV RNA with patient- and virus-associated factors. (eur.nl)
- Conclusion: In this multiethnic cohort of 488 untreated individuals with CHB, factors associated with serum HBV RNA level were HBeAg status, serum ALT, HBV genotype, and presence of basal core promotor mutations. (eur.nl)
- For the future use of serum HBV RNA as a clinical marker, it seems mandatory to take these factors into consideration. (eur.nl)
- DNA/RNA Shield ensures nucleic acid stability during sample storage/transport at ambient temperatures (4°C-25°C). The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). (zymoresearch.com)
- Target a specific viral RNA or DNA sequence and capture only that specific nucleic acid sequence directly from crude lysates or other biological fluids. (thermofisher.com)
- These Dynabeads® have an optimized surface chemistry for viral nucleic acid purification and provide efficient kinetics and a high sensitivity. (thermofisher.com)
- The nested PCR amplified integrated proviral DNA in nucleic acid extracted from plasma in PPT and EDTA specimens with high viral load values. (asm.org)
- Viral nucleic acid was isolated with Omega Bio-tek's Mag-Bind® Viral DNA/RNA Kit and with a comparable kit from Company A according to recommended protocols. (omegabiotek.com)
- Different RMs are expected to produce different evolutionary and dynamical outcomes in viral quasi-species due to differences in the mutations accumulation rate. (springer.com)
- Mutations within the 5′-UTR affecting RNA structural elements enabled restriction by or antagonism of Ifit1 in vitro and in vivo. (sciencemag.org)
- 3 Except for the NSW03 and NSW01 isolates, viral mutations are located at the stem loop-II (s2m) motif, an extremely conserved RNA element in the 3' untranslated region (Figure 1A). (mja.com.au)
- The first analysis included 29 patients receiving either monotherapy or combination therapy with the protease inhibitor ritonavir whose plasma HIV RNA levels rebounded from the point of greatest decline with mutations associated with resistance to ritonavir. (ovid.com)
- Since RNA-dependent RNA polymerase does not have an error-checking function, many mutations are made when the RNA is processed. (wikipedia.org)
- Sequencing of several cDNA clones derived from 6.0-kbp dsRNA revealed the presence of a RNA-dependent RNA polymerase (RDRP) gene. (apsnet.org)
- Both toxin and resistance substances are produced by processing of the single preprotoxin polypeptide produced by translation of viral transcripts of M dsRNA. (genetics.org)
- Upon placing an MDA5-RNA mixture under a microscope, they observed formation of MDA5 filaments along the dsRNA. (yalescientific.org)
- In each positive-strand RNA virus, most of the viral genes are devoted to this single process of RNA reproduction. (technologynetworks.com)
- The result is that the further downstream genes are from the leader sequence, the less they will be transcribed by RNA polymerase. (wikipedia.org)
- Reverse genetics is a powerful tool to study and characterize the previously unknown viral genes. (wikipedia.org)
- This system allows the investigators to explore the functional properties of individual viral genes of VHSV in detail. (wikipedia.org)
- In it, you mention: "DAS181, or Fludase, is unique in that it incorporates a sialidase that removes sialic acids from mucosal membranes, thereby preventing viral attachment via the HA glycoprotein. (virology.ws)
- Viral analysis of biological and environmental samples requires the use of advanced technologies to assure assay effectiveness. (thermofisher.com)
- To overcome these fabrication issues, the use of naturally occurring biological materials - such as RNA or DNA - for drug formulation may become the next big step in nanoparticulate therapy development. (nanowerk.com)
- The researchers also discovered the biological nano-switch that turns on transcription - the process by which RNA self-replicates - and compared the switch's structure in the "off" and "on" states to determine why environmental conditions activate it. (ucla.edu)
- Utilizing a biological system of virally infected mammalian cells, the substrate targeted in the study are viral RNA-protein complexes. (illinois.edu)
- Quantitative analysis reveals a new pause state that modulates polymerase fidelity and so ties viral polymerase pausing to the biological function of optimizing virulence. (tudelft.nl)
- The existence of these vast quantities of non-coding RNAs in cells implies the significance of these differential RNA species in the regulation of biological processes ( 1 , 4 ). (pubmedcentralcanada.ca)
Purification of high-quality1
Inhibition of viral1
- Further, this targeting results in inhibition of viral protein production. (pnas.org)
Isolation of viral1
- High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc. (zymoresearch.com)
- The same mathematical model for natural infections is here further analyzed, and we prove that the interior equilibrium involving coexistence of both positive and negative viral strands is globally asymptotically stable. (springer.com)
- Sept. 4, 2012) -- A new study by University of Kentucky researchers shows promise for developing ultrastable RNA nanoparticles that may help treat cancer and viral infections by regulating cell function and binding to cancers without harming surrounding tissue. (eurekalert.org)
- The overall goal of Sztuba-Solinska research is to find "weak-spots" in viral lncRNA interactome network that can be targeted with novel therapeutic strategies to combat viral infections. (auburn.edu)
- There is a significant need for new therapeutic approaches to combat diseases such as cancer and viral infections,' says Bruce A. Shapiro , Ph.D., a Senior Investigator at the Center for Cancer Research,National Cancer Institute (NCI). (nanowerk.com)
- However, research addressing the effect of host innate immune evasion on the pathology caused by viral infections is less prevalent in the literature, though very relevant and interesting. (mdpi.com)
- and viral infections have emerged as an imperative global hazard. (sigmaaldrich.com)
- Although virtually all cell types can produce type I IFN, plasmacytoid DCs are by far the most potent and are responsible for most of the systemically measurable IFN-α produced early during viral infections ( 2 ). (jimmunol.org)
- Induction and suppression of RNA silencing: insights from plant viral infections-a BARD. (deepdyve.com)
- Scholthof, Herman 2010-12-22 00:00:00 An international workshop on ''Induction and Suppression of RNA Silencing: Insights from Plant Viral Infections'' was sponsored by the United States-Israel Binational Agricultural Research and Development Fund (BARD) and organized in Eilat, Israel in March 2010. (deepdyve.com)
Amount of viral3
- Utilizing PHASIFY ™ VIRAL RNA extraction can result in 15-30-fold greater amount of viral RNA for standard RT-qPCR analyses,' says Klausner. (yahoo.com)
- In an attempt to answer that question, researchers at Ann & Robert H. Lurie Children's Hospital of Chicago measured the amount of viral load in the noses of 145 mildly to moderately ill COVID-19 patients, ranging in age from under 1 month to over 65 years of age. (flutrackers.com)
- they made very little viral protein, they did not inhibit host cell translation, and they synthesized a significant amount of viral RNA, although with some delay compared with wild type. (caltech.edu)
- Viral RNA extraction begins with lysis of the viral capsid from a variety of sample inputs, including saliva and nasopharyngeal or oropharyngeal swabs. (beckman.com)
- We conclude that Tsg101, through selective interactions with its partners including Hrs and Alix, may link receptor sorting and lysosome targeting to the back-fusion process involved in viral capsid release. (ovid.com)
- Is the Subject Area "Small interfering RNAs" applicable to this article? (plos.org)
- Using RNA as a therapeutic modality brings to bear an entirely new approach, which not only allows for the construction of uniform scaffolds for attachment of functional entities, but also permits the use of all the different types of functionalities that are inherent in natural RNAs. (nanowerk.com)
- Computationally designed RNA and RNA-DNA based nanorings presented in this work have multiple advantages in diagnostics and delivery of functional moieties to diseased cells,' Shapiro tells Nanowerk. (nanowerk.com)
- Figure 1: Functional RNA nanoparticles. (nanowerk.com)
- Extensive in vitro and in vivo characterizations of the resulting functional RNA nanoparticles were carried out at NCI in collaborations with Drs. Eric Freed s (NCI-Frederick), Jakob Reiser s (FDA), Wah Chiu s (Baylor College of Medicine), Luc Jaeger s (UCSB), Wade Grabow (SPU) laboratories. (nanowerk.com)
- Expression of N, the viral polymerase protein (L), and a minireplicon containing a reporter gene was sufficient to reconstitute functional virus nucleocapsids. (nih.gov)
- This vast difference between mRNA and non-protein coding RNA transcription suggests (a) the existence of an uncharacterized functional RNA-based regulation network and/or (b) the presence of a large quantity of non-functional-junk RNA transcripts within the cell. (pubmedcentralcanada.ca)
- Clustered, regularly interspaced, short palindromic repeats-CRISPR associated (CRISPR-Cas) systems are prokaryotic RNA-directed endonuclease machineries that act as an adaptive immune system against foreign genetic elements. (pnas.org)
- Next, Rana and team will investigate the role of RNA modifications in the viral life cycle, and how the human immune response is altered by various Zika virus strains. (eurekalert.org)
- In its fight against an intruding virus, an enzyme in our immune system may sense certain types of viral RNA pairs, according to scientists. (innovations-report.com)
- These results demonstrate that Sindbis virus can persist long term in a nonproductive form in mouse brain and suggest that the humoral immune response plays an important role in preventing viral reactivation. (asm.org)
- We demonstrate that octameric NSP2 binds structured RNAs with high affinity, resulting in efficient intramolecular RNA helix disruption. (uni-muenchen.de)
- Hexameric sigma NS oligomerizes into an octamer that binds two RNAs, yet it exhibits only limited RNA-unwinding activity compared to NSP2. (uni-muenchen.de)
- The Ebola virus VP35 protein binds viral immunostimulatory and host RNAs identified through deep sequencing. (jcvi.org)
- This is probably due to the advantage of having all RNA bound by N protein (since N binds hexamers of RNA). (wikipedia.org)
- Comparisons of the largest oligonucleotides derived by ribonuclease T1 digestion of these 40 S DEN virion RNA species indicate that there are few, if any, large oligonucleotides that are homologous between any two of the four dengue prototype strains. (ajtmh.org)
- Then, the chapter discusses the models that have been proposed to explain how viral positive and negative RNA species are made by the viral RNA-dependent RNA polymerase. (asmscience.org)
- Using the RNA nanotechnology pioneered by Guo, the researchers constructed ultrastable X-shaped RNA nanoparticles using re-engineered RNA fragments to carry up to four therapeutic and diagnostic modules. (eurekalert.org)
- We have addressed these issues, and now it is possible to produce RNA nanoparticles that are highly stable both chemically and thermodynamically in the test tube or in the body with great potential as therapeutic reagents. (eurekalert.org)
- To assess relative utility of viral load testing in determining therapeutic choice by the surrogate marker of CD4 cell counts after 48 weeks of therapy. (clinicaltrials.gov)
- It is hypothesized that among HIV-infected patients whose baseline CD4 count is in the range of 300 to 750 cells/mm3, those patients who incorporate initial and periodic viral RNA measurements in their therapeutic decisions will have higher CD4 counts after 48 weeks than patients whose therapeutic decisions do not incorporate initial and periodic viral RNA measurements. (clinicaltrials.gov)
- Elevated HIV-1 viral load (VL) observed in specimens frozen in situ in plasma preparation tubes (PPTs) compared to EDTA plasma specimens may affect therapeutic monitoring of HIV-infected patients. (asm.org)
- This has important implications for our understanding of the fundamental molecular biology of Picornavirales,and opens the door to novel research and therapeutic applications in the field of custom RNA packaging and delivery technologies. (jic.ac.uk)
- Notably, the first age-based multiscale mathematical model for HCV kinetics has been developed [ 25 , 40 , 41 ] providing a more comprehensive understanding of viral treatment response kinetics observed in patients treated with IFN, HCV protease inhibitors (telaprevir and danoprevir), or the HCV NS5A inhibitor daclatasvir as well as modes of action of these drugs. (frontiersin.org)
- We analyzed the viral RNA load kinetics of SARS-CoV-2 in various clinical specimens in children with COVID-19. (cdc.gov)
Designed to purify1
- 1 The key goal of ART is to achieve and maintain durable viral suppression. (nih.gov)
- If viral suppression is not possible, repeat viral load every 3 months or more frequently if indicated (AIII) . (nih.gov)
- Can extend to every 6 months for patients with consistent viral suppression for ≥2 years (AIII) . (nih.gov)
- We show that pathogenic alphaviruses use secondary structural motifs within the 5′-untranslated region (UTR) of their RNA to alter Ifit1 binding and function. (sciencemag.org)
- As such, this knowledge may also influence future strategies for the prevention and treatment of pathogenic viral diseases. (jbsdonline.com)
- Immunization of mice, chicken, pigs and primates with virus-like particles, naked RNA or layered DNA/RNA plasmids has provided protection against challenges with lethal doses of infectious agents and administered tumor cells. (mdpi.com)
- Viral hemorrhagic septicemia (VHS) is a deadly infectious fish disease caused by Piscine novirhabdovirus (originally called Viral hemorrhagic septicemia virus). (wikipedia.org)
- Cells can chemically modify RNA to influence protein production. (eurekalert.org)
- She grows infected cells, extracts total RNA and captures target PAN RNA with oligonucleotide probes. (auburn.edu)
- She has published one of only two papers that used SHAPE-MaP, a new method that allows researchers to probe the structure of any RNA of interest inside the living cells or within viral particle. (auburn.edu)
- In order to elucidate the function of NSs, we established a plasmid-based minireplicon system using mammalian cells that express large amounts of T7 RNA polymerase. (nih.gov)
- To evaluate, in HIV-infected patients whose baseline CD4 count is 300 to 750 cells/mm3, whether an antiretroviral treatment regimen based upon clinical evaluation and CD4 counts plus HIV RNA viral load is more effective than a treatment regimen based upon clinical evaluation and CD4 counts without the use of HIV RNA viral load information. (clinicaltrials.gov)
- yet, there are no data that identify viral immunostimulatory RNAs (isRNA) or host RNAs bound to VP35 in cells. (jcvi.org)
- The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. (tebu-bio.com)
- After isolation of cytoplasmic RNA from either infected or transfected cells and extraction of virus particle-associated RNA, specific RNA levels present in both fractions are determined. (bio-protocol.org)
- Add fresh medium to the transfected or infected cells and place them in the incubator until cytoplasmic RNA isolation. (bio-protocol.org)
- RNA was made from these constructs and used to transfect HeLa cells. (caltech.edu)
- We are a leading supplier of the most successful tools used for viral RNA and DNA capture, purification, and qRT-PCR and qPCR analyses. (thermofisher.com)
- Preliminary analyses of the 40 S RNA 5′ terminal sequence of DEN 3 virion RNA indicate that it has the composition of m7GpppAmpXp. (ajtmh.org)
- The findings were extended to a group of singly spliced viral mRNAs that produce Env in the following biochemical analyses. (nii.ac.jp)
- In both analyses, the durability of response was not highly associated with either baseline RNA or the magnitude of RNA decline from baseline. (ovid.com)