A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Ribonucleic acid that makes up the genetic material of viruses.
A process that changes the nucleotide sequence of mRNA from that of the DNA template encoding it. Some major classes of RNA editing are as follows: 1, the conversion of cytosine to uracil in mRNA; 2, the addition of variable number of guanines at pre-determined sites; and 3, the addition and deletion of uracils, templated by guide-RNAs (RNA, GUIDE).
The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Viruses whose genetic material is RNA.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.
Neoplasms containing cyst-like formations or producing mucin or serum.
RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.
The processes of RNA tertiary structure formation.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.
Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.
The extent to which an RNA molecule retains its structural integrity and resists degradation by RNASE, and base-catalyzed HYDROLYSIS, under changing in vivo or in vitro conditions.
A family of proteins that promote unwinding of RNA during splicing and translation.
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.
Tumors or cancer of the SKIN.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
RNA present in neoplastic tissue.
Two or more abnormal growths of tissue occurring simultaneously and presumed to be of separate origin. The neoplasms may be histologically the same or different, and may be found in the same or different sites.
Short chains of RNA (100-300 nucleotides long) that are abundant in the nucleus and usually complexed with proteins in snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR). Many function in the processing of messenger RNA precursors. Others, the snoRNAs (RNA, SMALL NUCLEOLAR), are involved with the processing of ribosomal RNA precursors.
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
RNA which does not code for protein but has some enzymatic, structural or regulatory function. Although ribosomal RNA (RNA, RIBOSOMAL) and transfer RNA (RNA, TRANSFER) are also untranslated RNAs they are not included in this scope.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Nucleic acid structures found on the 5' end of eukaryotic cellular and viral messenger RNA and some heterogeneous nuclear RNAs. These structures, which are positively charged, protect the above specified RNAs at their termini against attack by phosphatases and other nucleases and promote mRNA function at the level of initiation of translation. Analogs of the RNA caps (RNA CAP ANALOGS), which lack the positive charge, inhibit the initiation of protein synthesis.
Tumors or cancers of the KIDNEY.
A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE.
Abnormal growths of tissue that follow a previous neoplasm but are not metastases of the latter. The second neoplasm may have the same or different histological type and can occur in the same or different organs as the previous neoplasm but in all cases arises from an independent oncogenic event. The development of the second neoplasm may or may not be related to the treatment for the previous neoplasm since genetic risk or predisposing factors may actually be the cause.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Ribonucleic acid in plants having regulatory and catalytic roles as well as involvement in protein synthesis.
Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis.
Tumors or cancer of the THYROID GLAND.
An adenocarcinoma producing mucin in significant amounts. (From Dorland, 27th ed)
Conditions which cause proliferation of hemopoietically active tissue or of tissue which has embryonic hemopoietic potential. They all involve dysregulation of multipotent MYELOID PROGENITOR CELLS, most often caused by a mutation in the JAK2 PROTEIN TYROSINE KINASE.
An enzyme that catalyzes the conversion of linear RNA to a circular form by the transfer of the 5'-phosphate to the 3'-hydroxyl terminus. It also catalyzes the covalent joining of two polyribonucleotides in phosphodiester linkage. EC 6.5.1.3.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Tumors or cancer of the LUNG.
Tumors or cancer of the LIVER.
DNA present in neoplastic tissue.
Tumors or cancer of the PAROTID GLAND.
A large family of RNA helicases that share a common protein motif with the single letter amino acid sequence D-E-A-D (Asp-Glu-Ala-Asp). In addition to RNA helicase activity, members of the DEAD-box family participate in other aspects of RNA metabolism and regulation of RNA function.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 2.7.7.6.
A benign neoplasm derived from glandular epithelium, in which cystic accumulations of retained secretions are formed. In some instances, considerable portions of the neoplasm, or even the entire mass, may be cystic. (Stedman, 25th ed)
Neoplasms developing from some structure of the connective and subcutaneous tissue. The concept does not refer to neoplasms located in connective or soft tissue.
Neoplasms associated with a proliferation of a single clone of PLASMA CELLS and characterized by the secretion of PARAPROTEINS.
Tumors or cancer of the APPENDIX.
Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A multilocular tumor with mucin secreting epithelium. They are most often found in the ovary, but are also found in the pancreas, appendix, and rarely, retroperitoneal and in the urinary bladder. They are considered to have low-grade malignant potential.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. The enzyme functions in the nucleolar structure and transcribes DNA into RNA. It has different requirements for cations and salts than RNA polymerase II and III and is not inhibited by alpha-amanitin. EC 2.7.7.6.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
RNA molecules found in the nucleus either associated with chromosomes or in the nucleoplasm.
Tumors or cancer of the ENDOCRINE GLANDS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Small kinetoplastid mitochondrial RNA that plays a major role in RNA EDITING. These molecules form perfect hybrids with edited mRNA sequences and possess nucleotide sequences at their 5'-ends that are complementary to the sequences of the mRNA's immediately downstream of the pre-edited regions.
Constituent of the 60S subunit of eukaryotic ribosomes. 28S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
Tumors or cancer of the GASTROINTESTINAL TRACT, from the MOUTH to the ANAL CANAL.
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Neoplasms composed of vascular tissue. This concept does not refer to neoplasms located in blood vessels.
Tumors or cancer of the EYE.
Tumors or cancer of the NOSE.
Tumors or cancer of the SALIVARY GLANDS.
Tumors, cancer or other neoplasms produced by exposure to ionizing or non-ionizing radiation.
An adenocarcinoma containing finger-like processes of vascular connective tissue covered by neoplastic epithelium, projecting into cysts or the cavity of glands or follicles. It occurs most frequently in the ovary and thyroid gland. (Stedman, 25th ed)
A malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. (Stedman, 25th ed)
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
Neoplasms composed of glandular tissue, an aggregation of epithelial cells that elaborate secretions, and of any type of epithelium itself. The concept does not refer to neoplasms located in the various glands or in epithelial tissue.
Tumors or cancer of the TESTIS. Germ cell tumors (GERMINOMA) of the testis constitute 95% of all testicular neoplasms.
Neoplasms composed of muscle tissue: skeletal, cardiac, or smooth. The concept does not refer to neoplasms located in muscles.
A benign epithelial tumor with a glandular organization.
A malignant cystic or semisolid tumor most often occurring in the ovary. Rarely, one is solid. This tumor may develop from a mucinous cystadenoma, or it may be malignant at the onset. The cysts are lined with tall columnar epithelial cells; in others, the epithelium consists of many layers of cells that have lost normal structure entirely. In the more undifferentiated tumors, one may see sheets and nests of tumor cells that have very little resemblance to the parent structure. (Hughes, Obstetric-Gynecologic Terminology, 1972, p184)
Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.
Neoplasms located in the blood and blood-forming tissue (the bone marrow and lymphatic tissue). The commonest forms are the various types of LEUKEMIA, of LYMPHOMA, and of the progressive, life-threatening forms of the MYELODYSPLASTIC SYNDROMES.
Neoplasms of whatever cell type or origin, occurring in the extraskeletal connective tissue framework of the body including the organs of locomotion and their various component structures, such as nerves, blood vessels, lymphatics, etc.
Tumors or cancer of the UTERUS.
Tumors or cancer of the INTESTINES.
Methods which attempt to express in replicable terms the extent of the neoplasm in the patient.
A general term for various neoplastic diseases of the lymphoid tissue.
Neoplasms composed of sebaceous or sweat gland tissue or tissue of other skin appendages. The concept does not refer to neoplasms located in the sebaceous or sweat glands or in the other skin appendages.
Neoplasms located in the vasculature system, such as ARTERIES and VEINS. They are differentiated from neoplasms of vascular tissue (NEOPLASMS, VASCULAR TISSUE), such as ANGIOFIBROMA or HEMANGIOMA.
Tumors or cancer located in bone tissue or specific BONES.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
The process of moving specific RNA molecules from one cellular compartment or region to another by various sorting and transport mechanisms.
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Tumors or cancer of the PALATE, including those of the hard palate, soft palate and UVULA.
The small RNAs which provide spliced leader sequences, SL1, SL2, SL3, SL4 and SL5 (short sequences which are joined to the 5' ends of pre-mRNAs by TRANS-SPLICING). They are found primarily in primitive eukaryotes (protozoans and nematodes).
Small, linear single-stranded RNA molecules functionally acting as molecular parasites of certain RNA plant viruses. Satellite RNAs exhibit four characteristic traits: (1) they require helper viruses to replicate; (2) they are unnecessary for the replication of helper viruses; (3) they are encapsidated in the coat protein of the helper virus; (4) they have no extensive sequence homology to the helper virus. Thus they differ from SATELLITE VIRUSES which encode their own coat protein, and from the genomic RNA; (=RNA, VIRAL); of satellite viruses. (From Maramorosch, Viroids and Satellites, 1991, p143)
Neoplasms composed of more than one type of neoplastic tissue.
Tumors or cancer of the COLON.
Tumors or cancer of the BILE DUCTS.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Tumors or cancer of the MANDIBLE.
A malignant neoplasm derived from glandular epithelium, in which cystic accumulations of retained secretions are formed. The neoplastic cells manifest varying degrees of anaplasia and invasiveness, and local extension and metastases occur. Cystadenocarcinomas develop frequently in the ovaries, where pseudomucinous and serous types are recognized. (Stedman, 25th ed)
Tumors or cancer of the THYMUS GLAND.
Established cell cultures that have the potential to propagate indefinitely.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Tumors or cancer of the SPLEEN.
A cystic tumor of the ovary, containing thin, clear, yellow serous fluid and varying amounts of solid tissue, with a malignant potential several times greater than that of mucinous cystadenoma (CYSTADENOMA, MUCINOUS). It can be unilocular, parvilocular, or multilocular. It is often bilateral and papillary. The cysts may vary greatly in size. (Dorland, 27th ed; from Hughes, Obstetric-Gynecologic Terminology, 1972)
Tumors in any part of the heart. They include primary cardiac tumors and metastatic tumors to the heart. Their interference with normal cardiac functions can cause a wide variety of symptoms including HEART FAILURE; CARDIAC ARRHYTHMIAS; or EMBOLISM.
Cancer or tumors of the MAXILLA or upper jaw.
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
Ribonucleic acid in archaea having regulatory and catalytic roles as well as involvement in protein synthesis.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Tumors or cancer of the anal gland.
Neoplasms composed of primordial GERM CELLS of embryonic GONADS or of elements of the germ layers of the EMBRYO, MAMMALIAN. The concept does not refer to neoplasms located in the gonads or present in an embryo or FETUS.
Neoplasms located in the bone marrow. They are differentiated from neoplasms composed of bone marrow cells, such as MULTIPLE MYELOMA. Most bone marrow neoplasms are metastatic.
Neoplasms composed of fatty tissue or connective tissue made up of fat cells in a meshwork of areolar tissue. The concept does not refer to neoplasms located in adipose tissue.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Tumors or cancer of the MOUTH.
Benign and malignant neoplastic processes that arise from or secondarily involve the meningeal coverings of the brain and spinal cord.
Tumors or cancer of the COLON or the RECTUM or both. Risk factors for colorectal cancer include chronic ULCERATIVE COLITIS; FAMILIAL POLYPOSIS COLI; exposure to ASBESTOS; and irradiation of the CERVIX UTERI.
Tumors or cancer of the DUODENUM.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A reaction that severs one of the sugar-phosphate linkages of the phosphodiester backbone of RNA. It is catalyzed enzymatically, chemically, or by radiation. Cleavage may be exonucleolytic, or endonucleolytic.
A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Tumors or cancers of the ADRENAL CORTEX.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Nuclear nonribosomal RNA larger than about 1000 nucleotides, the mass of which is rapidly synthesized and degraded within the cell nucleus. Some heterogeneous nuclear RNA may be a precursor to mRNA. However, the great bulk of total hnRNA hybridizes with nuclear DNA rather than with mRNA.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Tumors or cancer of the STOMACH.
Tumors or cancer of the TONGUE.
Tumors or cancer of the MEDIASTINUM.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Small RNAs found in the cytoplasm usually complexed with proteins in scRNPs (RIBONUCLEOPROTEINS, SMALL CYTOPLASMIC).
Tumors or cancer of the URINARY BLADDER.
Tumors or cancer in the ILEUM region of the small intestine (INTESTINE, SMALL).
The steps that generate the 3' ends of mature RNA molecules. For most mRNAs (RNA, MESSENGER), 3' end processing referred to as POLYADENYLATION includes the addition of POLY A.
A malignant epithelial tumor with a glandular organization.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
Tumors or cancer of the human BREAST.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Short RNA, about 200 base pairs in length or shorter, that does not code for protein.
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
A malignant tumor arising from secreting cells of a racemose gland, particularly the salivary glands. Racemose (Latin racemosus, full of clusters) refers, as does acinar (Latin acinus, grape), to small saclike dilatations in various glands. Acinar cell carcinomas are usually well differentiated and account for about 13% of the cancers arising in the parotid gland. Lymph node metastasis occurs in about 16% of cases. Local recurrences and distant metastases many years after treatment are common. This tumor appears in all age groups and is most common in women. (Stedman, 25th ed; Holland et al., Cancer Medicine, 3d ed, p1240; from DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p575)
Benign and malignant neoplasms which occur within the substance of the spinal cord (intramedullary neoplasms) or in the space between the dura and spinal cord (intradural extramedullary neoplasms). The majority of intramedullary spinal tumors are primary CNS neoplasms including ASTROCYTOMA; EPENDYMOMA; and LIPOMA. Intramedullary neoplasms are often associated with SYRINGOMYELIA. The most frequent histologic types of intradural-extramedullary tumors are MENINGIOMA and NEUROFIBROMA.
Tumors or cancer of the VAGINA.
The local recurrence of a neoplasm following treatment. It arises from microscopic cells of the original neoplasm that have escaped therapeutic intervention and later become clinically visible at the original site.
Benign and malignant neoplastic processes arising from or involving components of the central, peripheral, and autonomic nervous systems, cranial nerves, and meninges. Included in this category are primary and metastatic nervous system neoplasms.
A usually benign glandular tumor composed of oxyphil cells, large cells with small irregular nuclei and dense acidophilic granules due to the presence of abundant MITOCHONDRIA. Oxyphil cells, also known as oncocytes, are found in oncocytomas of the kidney, salivary glands, and endocrine glands. In the thyroid gland, oxyphil cells are known as Hurthle cells and Askanazy cells.
A Janus kinase subtype that is involved in signaling from GROWTH HORMONE RECEPTORS; PROLACTIN RECEPTORS; and a variety of CYTOKINE RECEPTORS such as ERYTHROPOIETIN RECEPTORS and INTERLEUKIN RECEPTORS. Dysregulation of Janus kinase 2 due to GENETIC TRANSLOCATIONS have been associated with a variety of MYELOPROLIFERATIVE DISORDERS.
Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.
A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
Constituent of the 60S subunit of eukaryotic ribosomes. 5.8S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
Tumors or cancer located in muscle tissue or specific muscles. They are differentiated from NEOPLASMS, MUSCLE TISSUE which are neoplasms composed of skeletal, cardiac, or smooth muscle tissue, such as MYOSARCOMA or LEIOMYOMA.
Experimentally induced tumors of the LIVER.
A rare malignant neoplasm characterized by rapidly proliferating, extensively infiltrating, anaplastic cells derived from blood vessels and lining irregular blood-filled or lumpy spaces. (Stedman, 25th ed)
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A class of untranslated RNA molecules that are typically greater than 200 nucleotides in length and do not code for proteins. Members of this class have been found to play roles in transcriptional regulation, post-transcriptional processing, CHROMATIN REMODELING, and in the epigenetic control of chromatin.
Small nuclear RNAs that are involved in the processing of pre-ribosomal RNA in the nucleolus. Box C/D containing snoRNAs (U14, U15, U16, U20, U21 and U24-U63) direct site-specific methylation of various ribose moieties. Box H/ACA containing snoRNAs (E2, E3, U19, U23, and U64-U72) direct the conversion of specific uridines to pseudouridine. Site-specific cleavages resulting in the mature ribosomal RNAs are directed by snoRNAs U3, U8, U14, U22 and the snoRNA components of RNase MRP and RNase P.
Clonal myeloid disorders that possess both dysplastic and proliferative features but are not properly classified as either MYELODYSPLASTIC SYNDROMES or MYELOPROLIFERATIVE DISORDERS.
Surgical removal of the pancreas. (Dorland, 28th ed)
Neoplasms which arise from peripheral nerve tissue. This includes NEUROFIBROMAS; SCHWANNOMAS; GRANULAR CELL TUMORS; and malignant peripheral NERVE SHEATH NEOPLASMS. (From DeVita Jr et al., Cancer: Principles and Practice of Oncology, 5th ed, pp1750-1)
Neoplasms of the thin serous membrane that envelopes the lungs and lines the thoracic cavity. Pleural neoplasms are exceedingly rare and are usually not diagnosed until they are advanced because in the early stages they produce no symptoms.
Neoplasms located in the brain ventricles, including the two lateral, the third, and the fourth ventricle. Ventricular tumors may be primary (e.g., CHOROID PLEXUS NEOPLASMS and GLIOMA, SUBEPENDYMAL), metastasize from distant organs, or occur as extensions of locally invasive tumors from adjacent brain structures.
Tumors or cancer of the PARANASAL SINUSES.
Tomography using x-ray transmission and a computer algorithm to reconstruct the image.
Tumor or cancer of the COMMON BILE DUCT including the AMPULLA OF VATER and the SPHINCTER OF ODDI.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Synthetic transcripts of a specific DNA molecule or fragment, made by an in vitro transcription system. This cRNA can be labeled with radioactive uracil and then used as a probe. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Primary or metastatic neoplasms of the CEREBELLUM. Tumors in this location frequently present with ATAXIA or signs of INTRACRANIAL HYPERTENSION due to obstruction of the fourth ventricle. Common primary cerebellar tumors include fibrillary ASTROCYTOMA and cerebellar HEMANGIOBLASTOMA. The cerebellum is a relatively common site for tumor metastases from the lung, breast, and other distant organs. (From Okazaki & Scheithauer, Atlas of Neuropathology, 1988, p86 and p141)
Neoplasms of the bony orbit and contents except the eyeball.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
A benign tumor composed of fat cells (ADIPOCYTES). It can be surrounded by a thin layer of connective tissue (encapsulated), or diffuse without the capsule.
A family of enzymes that catalyze the endonucleolytic cleavage of RNA. It includes EC 3.1.26.-, EC 3.1.27.-, EC 3.1.30.-, and EC 3.1.31.-.
A collective term for precoordinated organ/neoplasm headings locating neoplasms by organ, as BRAIN NEOPLASMS; DUODENAL NEOPLASMS; LIVER NEOPLASMS; etc.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
Tumors or cancer of the PERITONEUM.
Tumors or cancer of the BRONCHI.
Distinctive neoplastic disorders of histiocytes. Included are malignant neoplasms of MACROPHAGES and DENDRITIC CELLS.
Tumors or cancer of the UROGENITAL SYSTEM in either the male or the female.
Ribonucleic acid in chloroplasts having regulatory and catalytic roles as well as involvement in protein synthesis.
Tumors or cancer of the VULVA.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Elements of limited time intervals, contributing to particular results or situations.
Neoplasms of the bony part of the skull.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The rate dynamics in chemical or physical systems.

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (1/4337)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

Differential stability of the DNA-activated protein kinase catalytic subunit mRNA in human glioma cells. (2/4337)

DNA-dependent protein kinase (DNA-PK) functions in double-strand break repair and immunoglobulin [V(D)J] recombination. We previously established a radiation-sensitive human cell line, M059J, derived from a malignant glioma, which lacks the catalytic subunit (DNA-PKcs) of the DNA-PK multiprotein complex. Although previous Northern blot analysis failed to detect the DNA-PKcs transcript in these cells, we show here through quantitative studies that the transcript is present, albeit at greatly reduced (approximately 20x) levels. Sequencing revealed no genetic alteration in either the promoter region, the kinase domain, or the 3' untranslated region of the DNA-PKcs gene to account for the reduced transcript levels. Nuclear run-on transcription assays indicated that the rate of DNA-PKcs transcription in M059J and DNA-PKcs proficient cell lines was similar, but the stability of the DNA-PKcs message in the M059J cell line was drastically (approximately 20x) reduced. Furthermore, M059J cells lack an alternately spliced DNA-PKcs transcript that accounts for a minor (5-20%) proportion of the DNA-PKcs message in all other cell lines tested. Thus, alterations in DNA-PKcs mRNA stability and/or the lack of the alternate mRNA may result in the loss of DNA-PKcs activity. This finding has important implications as DNA-PKcs activity is essential to cells repairing damage induced by radiation or radiomimetric agents.  (+info)

Collagenase-3 (MMP-13) is expressed by tumor cells in invasive vulvar squamous cell carcinomas. (3/4337)

Collagenase-3 (MMP-13) is a human matrix metalloproteinase specifically expressed by invading tumor cells in squamous cell carcinomas (SCCs) of the head and neck. Here, we have further elucidated the role of MMP-13 in tumor invasion by examining its expression in invasive malignant tumors of the female genital tract. Using in situ hybridization, expression of MMP-13 mRNA was detected in 9 of 12 vulvar SCCs, primarily in tumor cells, but not in intact vulvar epithelium, in cervical SCCs (n = 12), or in endometrial (n = 11) or ovarian adenocarcinomas (n = 8). MMP-13 expression was especially abundant in vulvar carcinomas showing metastasis to lymph nodes and was associated with expression of membrane type 1 MMP by tumor cells and gelatinase-A (MMP-2) by stromal cells, as detected by immunohistochemistry. MMP-13 mRNAs were detected in 9 of 11 cell lines established from vulvar carcinomas and in 4 of 6 cell lines from cervical carcinomas, whereas endometrial (n = 10) and ovarian (n = 9) carcinoma cell lines were negative for MMP-13 mRNA. No correlation was detected between MMP-13 expression and p53 gene mutations in vulvar SCC cell lines. However, MMP-13 expression was detected in 5 of 6 vulvar and cervical SCC cell lines harboring HPV 16 or 68 DNA. These results show that MMP-13 is specifically expressed by malignantly transformed squamous epithelial cells, including vulvar SCC cells, and appears to serve as a marker for their invasive capacity.  (+info)

Specific chromosomal aberrations and amplification of the AIB1 nuclear receptor coactivator gene in pancreatic carcinomas. (4/4337)

To screen pancreatic carcinomas for chromosomal aberrations we have applied molecular cytogenetic techniques, including fluorescent in situ hybridization, comparative genomic hybridization, and spectral karyotyping to a series of nine established cell lines. Comparative genomic hybridization revealed recurring chromosomal gains on chromosome arms 3q, 5p, 7p, 8q, 12p, and 20q. Chromosome losses were mapped to chromosome arms 8p, 9p, 17p, 18q, 19p, and chromosome 21. The comparison with comparative genomic hybridization data from primary pancreatic tumors indicates that a specific pattern of chromosomal copy number changes is maintained in cell culture. Metaphase chromosomes from six cell lines were analyzed by spectral karyotyping, a technique that allows one to visualize all chromosomes simultaneously in different colors. Spectral karyotyping identified multiple chromosomal rearrangements, the majority of which were unbalanced. No recurring reciprocal translocation was detected. Cytogenetic aberrations were confirmed using fluorescent in situ hybridization with probes for the MDR gene and the tumor suppressor genes p16 and DCC. Copy number increases on chromosome 20q were validated with a probe specific for the nuclear receptor coactivator AIB1 that maps to chromosome 20q12. Amplification of this gene was identified in six of nine pancreatic cancer cell lines and correlated with increased expression.  (+info)

Detection of occult lymph node metastases in esophageal cancer by minimally invasive staging combined with molecular diagnostic techniques. (5/4337)

BACKGROUND AND OBJECTIVES: Lymph node metastases are the most important prognostic factor in patients with esophageal cancer. Histologic examination misses micrometastases in up to 20% of lymph nodes evaluated. In addition, non-invasive imaging modalities are not sensitive enough to detect small lymph nodes metastases. The objective of this study was to investigate the use of reverse transcriptase-polymerase chain reaction (RT-PCR) of messenger RNA (mRNA) for carcinoembryonic antigen (CEA) to increase the detection of micrometastases in lymph nodes from patients with esophageal cancer. METHODS: RT-PCR of CEA mRNA was performed in lymph nodes from patients with malignant and benign esophageal disease. Each specimen was examined histopathologically and by RT-PCR and the results were compared. RESULTS: Metastases were present in 29 of 60 (48%) lymph nodes sample by minimally invasive staging from 13 patients with esophageal cancer when examined histopathologically. RT-PCR identified nodal metastases in 46 of these 60 (77%) samples. RT-PCR detected CEA mRNA in all 29 histologically positive samples and in 17 histologically negative lymph nodes. All lymph nodes from patients with benign disease (n = 15) were negative both histopathologically and by RT-PCR. The stage of two patients was reclassified based on the RT-PCR results, which identified lymph node spread undetected histopathologically. Both of these patients developed recurrent disease after resection of the primary tumor. CONCLUSIONS: RT-PCR is more sensitive than histologic examination in the detection of lymph node metastases in esophageal cancer and can lead to diagnosis of a more advanced stage in some patients. The combination of minimally invasive surgical techniques in combination with new molecular diagnostic techniques may improve our ability to stage cancer patients.  (+info)

Impact of 9-(2-phosphonylmethoxyethyl)adenine on (deoxy)ribonucleotide metabolism and nucleic acid synthesis in tumor cells. (6/4337)

Following exposure to 9-(2-phosphonylmethoxyethyl)adenine (an inhibitor of the cellular DNA polymerases alpha, delta and epsilon), human erythroleukemia K562, human T-lymphoid CEM and murine leukemia L1210 cells markedly accumulated in the S phase of the cell cycle. In contrast to DNA replication, RNA synthesis (transcription) and protein synthesis (mRNA translation) were not affected by 9-(2-phosphonylmethoxyethyl)-adenine. The ribonucleoside triphosphate pools were slightly elevated, while the intracellular levels of all four deoxyribonucleoside triphosphates were 1.5-4-fold increased in 9-(2-phosphonylmethoxyethyl)adenine-treated K562, CEM and L1210 cells. The effect of 9-(2-phosphonylmethoxyethyl)adenine on de novo (thymidylate synthase-mediated) and salvage (thymidine kinase-mediated) dTTP synthesis was investigated using radio-labelled nucleoside precursors. The amount of thymidylate synthase-derived dTTP in the acid soluble pool was 2-4-fold higher in PMEA-treated than in untreated K562 cells, which is in accord with the 3-4-fold expansion of the global dTTP level in the presence of 9-(2-phosphonylmethoxyethyl)adenine. Strikingly, 2-derived dTTP accumulated to a much higher extent (i.e. 16-40-fold) in the soluble dTTP pool upon 9-(2-phosphonylmethoxyethyl)adenine treatment. In keeping with this finding, a markedly increased thymidine kinase activity could be demonstrated in extracts of 9-(2-phosphonylmethoxyethyl)adenine-treated K562 cell cultures. Also, in the presence of 200 microM 9-(2-phosphonylmethoxyethyl)adenine, 14-fold less thymidylate synthase-derived but only 3-fold less thymidine kinase-derived dTTP was incorporated into the DNA of the K562 cells. These data show that thymidine incorporation may be inappropriate as a cell proliferation marker in the presence of DNA synthesis inhibitors such as 9-(2-phosphonylmethoxyethyl)adenine. Our findings indicate that 9-(2-phosphonylmethoxyethyl)adenine causes a peculiar pattern of (deoxy)ribonucleotide metabolism deregulation in drug-treated tumor cells, as a result of the metabolic block imposed by the drug on the S phase of the cell cycle.  (+info)

Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells. (7/4337)

Reverse transcriptase polymerase chain reaction (RT-PCR) is increasingly used to detect small numbers of circulating tumour cells, though the clinical benefit remains controversial. The largest single contributing factor to the controversy of its value is the different approaches to sample processing. The aim of this study was to compare the sensitivity and reproducibility of RT-PCR for the detection of tumour cells after four commonly used different methods of sample processing. Using RT-PCR, one tumour cell spiked in 2 ml of whole blood was detected after analysis of separated mononuclear cell RNA, whole blood total or poly-A+ RNA. No false positives were identified with any method. However, the reproducibility of tumour cell detection was reduced after isolation of the mononuclear cell fraction. Only analysis of poly-A+ RNA had a sensitivity of 100% in all the cell spiking experiments. In patient blood samples, analysis of poly-A+ RNA increased the number of blood samples positive for tyrosine hydroxylase (TH) mRNA compared with those positive after analysis of total RNA. This may reflect high levels of cDNA reducing the efficiency of the PCR. Isolation of poly-A+ RNA increases the sensitivity and reproducibility of tumour cell detection in peripheral blood.  (+info)

Atypical multidrug resistance: breast cancer resistance protein messenger RNA expression in mitoxantrone-selected cell lines. (8/4337)

BACKGROUND: Human cancer cell lines grown in the presence of the cytotoxic agent mitoxantrone frequently develop resistance associated with a reduction in intracellular drug accumulation without increased expression of the known drug resistance transporters P-glycoprotein and multidrug resistance protein (also known as multidrug resistance-associated protein). Breast cancer resistance protein (BCRP) is a recently described adenosine triphosphate-binding cassette transporter associated with resistance to mitoxantrone and anthracyclines. This study was undertaken to test the prevalence of BCRP overexpression in cell lines selected for growth in the presence of mitoxantrone. METHODS: Total cellular RNA or poly A+ RNA and genomic DNA were isolated from parental and drug-selected cell lines. Expression of BCRP messenger RNA (mRNA) and amplification of the BCRP gene were analyzed by northern and Southern blot hybridization, respectively. RESULTS: A variety of drug-resistant human cancer cell lines derived by selection with mitoxantrone markedly overexpressed BCRP mRNA; these cell lines included sublines of human breast carcinoma (MCF-7), colon carcinoma (S1 and HT29), gastric carcinoma (EPG85-257), fibrosarcoma (EPF86-079), and myeloma (8226) origins. Analysis of genomic DNA from BCRP-overexpressing MCF-7/MX cells demonstrated that the BCRP gene was also amplified in these cells. CONCLUSIONS: Overexpression of BCRP mRNA is frequently observed in multidrug-resistant cell lines selected with mitoxantrone, suggesting that BCRP is likely to be a major cellular defense mechanism elicited in response to exposure to this drug. It is likely that BCRP is the putative "mitoxantrone transporter" hypothesized to be present in these cell lines.  (+info)

Recently, dendritic cells (DC) transfected with tumor RNA have been used as a cancer vaccine. The efficacy of a cancer vaccine using DC transfected tumor RNA was examined. Of particular interest was whether a vaccine using DC transfected with recrudescent tumor RNA is effective for the treatment of a regrowing tumor after prior immunotherapy. In addition, the usefulness of co-transfection of granulocyte macrophage colony-stimulating factor (GM-CSF) mRNA to augment the DC vaccine was examined. CT26 tumor-bearing mice were immunized by s.c. injection with DC transfected with CT26 mRNA (DC-CT26). The cytotoxic activity against CT26 in mice immunized with DC-CT26 was significantly higher than that in the control group (P , 0.001) and was augmented by GM-CSF mRNA co-transfection (P , 0.05), resulting in remarkable therapeutic efficacy in CT26 s.c. tumor models. Cytotoxic T lymphocytes induced by the vaccination using DC transfected with mRNA from the recrudescent tumor showed a potent cytotoxicity ...
Caruso, Denise A, Orme, Lisa M, Neale, Alana M, Radcliff, Fiona J, Amor, Gerlinda M, Maixner, Wirginia, Downie, Peter, Hassall, Timothy E, Tang, Mimi LK and Ashley, David M 2004, Results of a phase 1 study utilizing monocyte-derived dendritic cells pulsed with tumor RNA in children and young adults with brain cancer, Neuro-oncology, vol. 6, no. 3, pp. 236-246, doi: 10.1215/S1152851703000668. ...
0110] The following references, to the extent that they provide exemplary procedural or other details supplementary to those set forth herein, are specifically incorporated herein by reference. [0111] U.S. Pat. No. 5,922,591 [0112] U.S. Pat. No. 5,921,396 [0113] U.S. Pat. No. 5,726,012 [0114] U.S. Pat. No. 5,090,420 [0115] Allewell, N. M., Sama, A., The effect of ammonium sulfate on the activity of ribonuclease A. Biochemica et Biophysica Acta 341:484-488 (1974). [0116] Auffray, C., Rougeon, F., Purification of mouse immunoglobulin heavy chain messenger RNAs from total myeloma tumor RNA. Eur. J. Biochemistry June;107(2)303-314 (1980). [0117] Boom, W. R., Adriaanse, H., Kievits, T., Lens, P. F., U. S. Pat. No. 5,234,809 entitled: Process for Isolating Nucleic Acid. [0118] Bugos R. C., Chiang, V. L., Zhang, X. H., Campbell, E. R., Podila G. K., Campbell W. H., RNA isolation from plant tissues recalcitrant to extraction by guanidine. Biotechniques November;19(5)734-7 (1995). [0119] Cairns, M. ...
My 5 year old St. Bernard was recently diagnosed with Osteosarcoma. They dont give him long at all, but we want to make him as happy and comfortable as
In another study titled See the Change, researchers at the Genomic Research Institute have been working on a method called liquid biopsy through which better diagnosis and monitoring of the DIPG tumor can be made possible. It can also help track DIPG in real time and measure the impact of treatment on the tumor much before MRI scans can.. A research, supported by Michigan Medicines Pediatric Brain Tumor Research Initiative found that a gene mutation known as PTEN plays an important role in DIPGs course in the brain stem. Targetting PTEN may be the path to finding a cure.. Scientists at the Feinberg School of Medicine of Northwestern University have found a molecule that could stop the development of DIPG. BET bromodomain inhibitor, the molecule used in this study, was effective in stopping the growth of the tumor. More trials are yet to be done to prove its efficacy.. The BRAVO trial where the clinical effects of a personalized dendritic cell vaccine made from the persons tumor RNA ...
Thus far Ive discussed various methods we use to diagnose dogs with osteosarcoma and the staging tests for canine osteosarcoma. In the following two articles I will describe palliative and definitive treatment options for this disease, and their respective prognoses. To review, osteosarcoma is an aggressive form of bone cancer in dogs. Most tumors arise…
2007). "Expression of the RNA-binding protein VICKZ in normal hematopoietic tissues and neoplasms". Haematologica. 92 (2): 176- ... Zhang JY, Chan EK, Peng XX, Tan EM (May 1999). "A novel cytoplasmic protein with RNA-binding motifs is an autoantigen in human ... a multi-talented family of regulatory RNA-binding proteins". Biol. Cell. 97 (1): 87-96. doi:10.1042/BC20040151. PMID 15601260. ...
Dalia S, Shao H, Sagatys E, Cualing H, Sokol L (October 2014). "Dendritic cell and histiocytic neoplasms: biology, diagnosis, ... Skalsky RL, Cullen BR (2015). "EBV Noncoding RNAs". Epstein Barr Virus Volume 2. Current Topics in Microbiology and Immunology ... Tang VK, Vijhani P, Cherian SV, Ambelil M, Estrada-Y-Martin RM (2018). "Primary pulmonary lymphoproliferative neoplasms". Lung ... presence of EBV RNA (e.g. an EBER) in an afflicted organ or tissue; and 5) occurrence of these findings in individuals who do ...
Folic acid is essential for the body to make DNA, RNA, and metabolise amino acids, which are required for cell division. Not ... Folate deficiency hinders DNA synthesis and cell division, affecting hematopoietic cells and neoplasms the most because of ... RNA transcription and subsequent protein synthesis are less affected by folate deficiency, as the mRNA can be recycled and used ... Folate is necessary for the production and maintenance of new cells, for DNA synthesis and RNA synthesis through methylation, ...
It works by inhibiting the synthesis of RNA and DNA of carcinoma cells. It is a competitive inhibitor of cell-free RNA and DNA ... Anthramycin has been shown particularly effective against sarcomas, lymphomas, and gastrointestinal neoplasms.[citation needed ...
ORF74 - vGPCR ORF75 - FGARAT PAN, polyadenylated nuclear RNA - non-coding linear and circular RNAs miRNAs (mirKs) - viral ... Chen BJ, Chuang SS (March 2020). "Lymphoid Neoplasms With Plasmablastic Differentiation: A Comprehensive Review and Diagnostic ... "Discovery of Kaposi's sarcoma herpesvirus-encoded circular RNAs and a human antiviral circular RNA". Proceedings of the ... "Comparative Analysis of Gammaherpesvirus Circular RNA Repertoires: Conserved and Unique Viral Circular RNAs". Journal of ...
... transcribes viral RNA into the full length DNA complement. Rous P (September 1910). "A Transmissible Avian Neoplasm (Sarcoma of ... 4S RNA is the primer for RSV and 70S RNA serves as the template for DNA synthesis. Reverse transcriptase, an RNA-dependent DNA ... "Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer". J ... RNA. 12 (1): 102-10. doi:10.1261/rna.2129806. PMC 1370890. PMID 16301601. Weil JE, Hadjithomas M, Beemon KL (March 2009). " ...
RNA transcription and subsequent protein synthesis are less affected by folate deficiency, as the mRNA can be recycled and used ... Folate deficiency hinders DNA synthesis and cell division, affecting hematopoietic cells and neoplasms the most because of ... Folate is required for the body to make DNA and RNA and metabolise amino acids necessary for cell division. As humans cannot ... Folates are essential for the synthesis of DNA, the modification of DNA and RNA, the synthesis of methionine from homocysteine ...
... rna, neoplasm MeSH D13.444.735.628 - rna, nuclear MeSH D13.444.735.628.806 - rna, heterogeneous nuclear MeSH D13.444.735.628. ... rna, small nucleolar MeSH D13.444.735.635 - rna, plant MeSH D13.444.735.635.575 - rna, chloroplast MeSH D13.444.735.640 - rna ... rna, small interfering MeSH D13.444.735.300 - rna, archaeal MeSH D13.444.735.473 - rna, bacterial MeSH D13.444.735.476 - rna, ... rna, double-stranded MeSH D13.444.735.500 - rna, fungal MeSH D13.444.735.520 - rna, helminth MeSH D13.444.735.544 - rna, ...
In molecular biology mir-25 microRNA is a short RNA molecule. MicroRNAs function to regulate the expression levels of other ... MicroRNA expression pattern in intraductal papillary mucinous neoplasm]". The Korean Journal of Gastroenterology = Taehan ... mir-25 levels increase in human heart failure, and treatment with an anti-sense RNA molecule (antagomiR) was recently reported ... Anton R, Chatterjee SS, Simundza J, Cowin P, Dasgupta R (2011). Bellusci S (ed.). "A systematic screen for micro-RNAs ...
The binding of tetracyclines to cellular dsRNA (double stranded RNA) may be an explanation for their wide range of effect. It ... diabetes and various types of neoplasms. It has been shown that tetracyclines are not only active against broad spectrum of ... Like minocycline, tigecycline binds to the bacterial 30S ribosome, blocking the entry of transfer RNA. This ultimately prevents ... blocking tetracyclines from binding to the ribosome binding to the ribosome and distorting the structure to still allow t-RNA ...
It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. IMPDH2 ... been identified as an intracellular target of the natural product sanglifehrin A This gene is up-regulated in some neoplasms, ...
On the contrary, in some neoplasms CD44 upregulation is associated with a favorable outcome. This is true of prostate cancer, ... the exclusion of the v5 exon through alternative splicing was associated with the presence of RNA binding protein KHDRBS1 and ... and CD44-soluble proteins markedly reduces the malignant activities of various neoplasms, stressing the therapeutic potential ...
They form a subset of neoplasms. A neoplasm or tumor is a group of cells that have undergone unregulated growth and will often ... In addition, frequent epigenetic alterations of the DNA sequences code for small RNAs called microRNAs (or miRNAs). miRNAs do ...
In molecular biology mir-552 microRNA is a short RNA molecule. MicroRNAs function to regulate the expression levels of other ... MicroRNA expression pattern in intraductal papillary mucinous neoplasm]". The Korean Journal of Gastroenterology = Taehan ...
The H19 gene codes for a 2.3 kb RNA product. It is transcribed by RNA polymerase II, spliced and polyadenylated, but it does ... In contrast to most other cancers, adrenocortical neoplasms appear to have decreased expression of H19. To determine a possible ... suggest that the accumulation of H19 RNA in skeletal muscle cells is solely due to the stabilization of that RNA in the muscle ... The presence of IGF2 RNA expression when H19 RNA was downregulated provides further evidence that IGF2 expression is tightly ...
... lymphoid neoplasm diffuse large B-cell lymphoma, pancreatic adenocarcinoma, rectum adenocarcinoma, skin cutaneous melanoma, and ... MORT (Mortal Obligate RNA Transcript (also known as ZNF667-AS1)) is a long non-coding RNA (lncRNA) of the intergenic type ( ... Using data from human cancers curated in TCGA, MORT RNA expression and DNA methylation state were evaluated in the 10 most ... MORT has orthologs only in great apes - chimpanzees, gorillas and orangutans, and the RNA expression data indicates that the ...
It works by blocking the formation of DNA and RNA. Chlorambucil was approved for medical use in the United States in 1957. It ... trophoblastic neoplasms, and ovarian carcinoma. Moreover, it also has been used as an immunosuppressive drug for various ... preventing DNA synthesis and RNA transcription from the affected DNA. DNA damage via the formation of cross-links which ...
Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA ... This includes Wilms tumor, rhabdomyosarcoma, Ewing's sarcoma, trophoblastic neoplasm, testicular cancer, and certain types of ... It is believed to work by blocking the creation of RNA. Dactinomycin was approved for medical use in the United States in 1964 ...
Telomerase RNA as a detection marker in the serum of breast cancer patients. Clin. Cancer Res. 6, 3823-3826". www.researchgate. ... began to study the phenomenon of neoplasms in plants. Building on early grafting studies in plants as well as work by other ... In an article published in Science, November 10, 1972, bacterial RNA was demonstrated in frog brain cells after a bacterial ... Afterward, they found a high percentage of RNA-DNA hybridization between bacterial DNA extracted from bacteria of the same ...
The protein was identified during investigations on the origin of multiple myeloma, a B-cell hematologic neoplasm. To ... Using object oriented programming to model the interactions of 28 categories of molecules, including DNA, RNA, proteins, and ... DNA and/or RNA) specific for Mgen in a clinical specimen Current partners of individuals who tested positive for Mgen should be ... as the only viable option for detection of Mgen DNA or RNA. However, samples with positive NAAT for the pathogen should be ...
These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. Translin has been shown to ... to RNA". The Journal of Biological Chemistry. 276 (16): 13256-63. doi:10.1074/jbc.M009707200. PMID 11278549. Lee SP, Fuior E, ... complex which facilitates endonucleolytic cleavage of the passenger strand during microRNA loading into the RNA-induced ...
transcription initiation from RNA polymerase II promoter. • G1/S transition of mitotic cell cycle. • cytokine-mediated ... Neoplasm: Tumor suppressor genes/proteins and Oncogenes/Proto-oncogenes. Ligand. Growth factors. ... Zhang Z, Wang H, Li M, Rayburn E, Agrawal S, Zhang R (2005). "Novel MDM2 p53-independent functions identified through RNA ... regulation of transcription from RNA polymerase II promoter. • DNA damage response, signal transduction by p53 class mediator ...
RNA. 10 (2): 240-53. doi:10.1261/rna.5153204. PMC 1370536. PMID 14730023. Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R ... which is a myelodysplastic syndrome/myeloproliferative neoplasm). There is also an emerging body of evidence to suggest ... RNA. 10 (6): 929-41. doi:10.1261/rna.7320604. PMC 1370585. PMID 15146077. Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, ... Banerjee H, Rahn A, Gawande B, Guth S, Valcarcel J, Singh R (February 2004). "The conserved RNA recognition motif 3 of U2 snRNA ...
These genes include TAF2N (17q11) encoding an RNA-binding protein, that presents subunit of RNA polymerase II, TCF12 (15q21) ... EMC shows the smallest morphological variation between the tumors among all myxoid soft tissue neoplasms. The myxoid matrix has ... Extraskeletal myxoid chondrosarcoma (EMC) is a rare low-grade malignant mesenchymal neoplasm of the soft tissues, that differs ...
... in two different RNA synthetic processes performed by RNA-dependent RNA polymerase, which consists of L and P proteins: (1) ... Its expression is also increased in a wide range of other malignant neoplasms. Factor X (F10) is frequently expressed in normal ... The RNA-dependent RNA polymerase of the SeV consists of the large protein (L) and the phosphoprotein (P). The structural gene ... The genomic RNA functions as the template for the viral RNA transcription until the NP protein concentration increases. As the ...
Abed AA, Günther K, Kraus C, Hohenberger W, Ballhausen WG (November 2001). "Mutation screening at the RNA level of the STK11/ ... December 2001). "STK11/LKB1 Peutz-Jeghers gene inactivation in intraductal papillary-mucinous neoplasms of the pancreas". The ... and other neoplasms. However, the LKB1 gene was also found to be mutated in lung cancer of sporadic origin, predominantly ...
... in which a particular segment of DNA is copied into RNA by the enzyme RNA polymerase. Both RNA and DNA are nucleic acids, which ... RNA. See ribonucleic acid.. RNA polymerase. A member of a family of enzymes that are essential to life: they are found in all ... One of the four main nitrogenous bases found in both DNA and RNA, along with adenine, guanine, thymine, and uracil (in RNA); it ... messenger RNA. A large family of RNA molecules that convey genetic information from DNA to the ribosome.. metabolism. ...
In thyroid neoplasm, TAZ allows follicular adenomas to be distinguished from follicular carcinomas, while in cervical cancer ... "Characterization of a transgenic short hairpin RNA-induced murine model of Tafazzin deficiency". Human Gene Therapy. 22 (7): ... TAZ overexpression has been linked to rectal cancer, prostate cancer, thyroid neoplasm, and cervical cancer. In a study of 140 ... October 2004). "Gene expression profiling of differentiated thyroid neoplasms: diagnostic and clinical implications". Clinical ...
... familial dilated Cardiomyopathy due to anthracyclines Cardiomyopathy hearing loss type t RNA lysine gene mutation Hypertrophic ... Carrington syndrome Cartilage-hair hypoplasia Cartilage hair hypoplasia like syndrome Cartilaginous neoplasms Cartwright-Nelson ... hypoxia Cerebral malformations hypertrichosis claw hands Cerebral palsy Cerebral thrombosis Cerebral ventricle neoplasms ... Choriocarcinoma Chorioretinitis Chorioretinopathy dominant form microcephaly Choroid plexus cyst Choroid plexus neoplasms ...
It is an RNA synthesis inhibitor. The manufacturer discontinued production in 2000. Several different structures are currently ... Brown, John H.; Kennedy, B. J. (1965). "Mithramycin in the Treatment of Disseminated Testicular Neoplasms". New England Journal ...
RNA polymerase II core promoter sequence-specific DNA binding. • RNA polymerase II transcription factor activity, sequence- ... aka Hurthle-Cell Neoplasms).[15] Tumors expressing the PAX8/PPARy are usually present in at a young age, small in size, present ... transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding. • RNA polymerase ... transcription from RNA polymerase II promoter. • regulation of thyroid-stimulating hormone secretion. • transcription, DNA- ...
RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... 2005). "Assessment of JC polyoma virus in colon neoplasms". Dis ... RNA virus. HCV Hepatocellular carcinoma. Splenic marginal zone lymphoma. HTLV-I Adult T-cell leukemia/lymphoma. ... RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ...
RNA) - ribosome - RNA - route of administration - RT-PCR - RTI - Ryan White C.A.R.E. act ... neoplasm - nephrotoxic - neuralgia - neurological complications of AIDS - neuropathy - neutralization - neutralizing antibody ... messenger RNA - metabolism - metastasis - MHC - microbes - microbicide - Microsporidiosis - mitochondria - mitochondrial ...
Secondary neoplasm[edit]. Development of secondary neoplasia after successful chemotherapy or radiotherapy treatment can occur ... Many of them have a similar structure to the building blocks of DNA and RNA. The building blocks are nucleotides; a molecule ... The most common secondary neoplasm is secondary acute myeloid leukemia, which develops primarily after treatment with ... Actinomycin is a complex molecule that intercalates DNA and prevents RNA synthesis.[58] ...
RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... Nervous tissue tumors/NS neoplasm/Neuroectodermal tumor (ICD-O ... RNA virus. HCV Hepatocellular carcinoma. Splenic marginal zone lymphoma. HTLV-I Adult T-cell leukemia/lymphoma. ... RNA virus. MeV Subacute sclerosing panencephalitis. LCV Lymphocytic choriomeningitis. Arbovirus encephalitis. Orthomyxoviridae ...
MicroRNAs (miRNAs) are small endogenous RNAs that pair with sequences in messenger RNAs to direct post-transcriptional ... see malignant neoplasms). Thus, CpG island hyper/hypo-methylation in the promoters of DNA repair genes are likely central to ... CpG islands also occur frequently in promoters for functional noncoding RNAs such as microRNAs.[17] ...
"Neoplasms and cancer" has been chosen to reflect the fact that not all tumours are benign. The word "cancer" has been included ...
DNA damage (or RNA damage in the case of some virus genomes) appears to be a fundamental problem for life. As noted by Haynes,[ ... Carcinogenesis and Neoplasm) and reference[24]). Furthermore, the ability of HRR to accurately and efficiently repair double- ... Massie, HR; Samis, HV; Baird, MB (1972). "The kinetics of degradation of DNA and RNA by H2O2". Biochim Biophys Acta. 272 (4): ...
Bernatsky S, Clarke AE, Suissa S (February 2008). "Hematologic malignant neoplasms after drug exposure in rheumatoid arthritis ... It is believed to work by interfering with the duplication of DNA and the creation of RNA.[4] ... Myeloproliferative neoplasms, including acute leukemia, non-Hodgkin lymphoma and multiple myeloma, occurred in 5 of 119 ...
Neoplasm: Tumor suppressor genes/proteins and Oncogenes/Proto-oncogenes. Ligand. Growth factors. ... RNA expression pattern. More reference expression data. Gene ontology. Molecular function. • calcium ion binding. • protein ...
Tüümuse RNA sisaldab adeniini, guaniini, tsütosiini, uratsiili. NukleosiididRedigeeri. AdenosiinRedigeeri. Pikemalt artiklis ... Tseng-Tong Kuo, Classification of thymic epithelial neoplasms: a controversial issue coming to an end?, J.Cell.Mol.Med. 5. ... and RNA in situ hybridization, The Journal of Pathology, 197. väljaanne, nr 5, lk 684-688, august 2002, DOI: 10.1002/path.1141 ... Using the World Health Organization Classification of Thymic Epithelial Neoplasms to Describe CT Findings, AJR:179, oktoober ...
Chapter 107: Neoplasms of the lung. (编) Kasper DL, Hauser SL, Jameson JL, Fauci AS, Longo DL, Loscalzo J. Harrison's Principles ... 微RNA)调控的改变可能会导致抑癌基因的灭活[59]。 ... Immunohistochemistry for Assessment of Pulmonary and Pleural Neoplasms: A Review and Update. Int J Clin Exp Pathol. 2008, 1 (
Srikumar Chakravarthi; Baba Krishnan; Malathy Madhavan (1999). "Apoptosis and expression of p53 in colorectal neoplasms". ... for example the putative RNA-binding protein 3 (RBM3).[42] ... As summarized in the articles Carcinogenesis and Neoplasm, for ...
RNA polymerase II core promoter sequence-specific DNA binding. • DNA binding. • p53 binding. • DNA binding, bending. • RNA ... "GATA1 Expression in BCR/ABL1-negative Myeloproliferative Neoplasms". Annals of Laboratory Medicine. 38 (4): 296-305. doi: ... RNA polymerase II transcription factor activity, sequence-specific DNA binding. • RNA polymerase II distal enhancer sequence- ... RNA polymerase II regulatory region sequence-specific DNA binding. • transcriptional activator activity, RNA polymerase II core ...
It functions by inhibiting RNA and DNA synthesis. Ciclosporin is an immunosuppressive agent most often used in organ ... in association with an underlying neoplasm". A study concluded in 2009, summarized in 2010, surrounded the surgical removal of ...
Neoplasm: Tumor suppressor genes/proteins and Oncogenes/Proto-oncogenes. Ligand. Growth factors. ... RNA expression pattern. More reference expression data. Gene ontology. Molecular function. • transferase activity. • nucleotide ...
... with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.[13] ... RNA expression pattern. More reference expression data. Gene ontology. Molecular function. • dipeptidyl-peptidase activity. • ...
... which may be benign neoplasms) or else a malignant neoplasm (cancer). These neoplasms are also indicated, in the diagram below ... microRNAs either cause degradation of messenger RNAs or block their translation)[56] For instance, hypomethylation of the ... The Hallmarks of Cancer as evolutionary adaptations in a neoplasm[edit]. In their landmark paper, The Hallmarks of Cancer,[3] ... Cells in neoplasms compete for resources, such as oxygen and glucose, as well as space. Thus, a cell that acquires a mutation ...
Riset terbaru menunjukkan bawa RNA pendek (small RNA) sepanjang 21-25 nukelotida yang dikenal sebagai RNA mikro (miRNA) dapat ... "A transmissible avian neoplasm. (Sarcoma of the common fowl) by Peyton Rous, M.D., Experimental Medicine for Sept. 1, 1910, vol ...
The binding of tetracyclines to cellular dsRNA (double stranded RNA) may be an explanation for their wide range of effect. It ... diabetes and various types of neoplasms.[24][25][26] It has been shown that tetracyclines are not only active against broad ... binding to the ribosome and distorting the structure to still allow t-RNA binding while tetracycline is bound[37] ... Like minocycline, tigecycline binds to the bacterial 30S ribosome, blocking the entry of transfer RNA. This ultimately prevents ...
... and a number of neoplasms of the bone marrow.[1] Causes of increased breakdown include a number of genetic conditions such as ... The cause of megaloblastic anemia is primarily a failure of DNA synthesis with preserved RNA synthesis, which results in ...
Salivary gland neoplasms *Benign: Basal cell adenoma. *Canalicular adenoma. *Ductal papilloma. *Monomorphic adenoma ...
negative regulation of transcription from RNA polymerase II promoter. • peptidyl-lysine modification. • response to drug. • ... Neoplasm: Tumor suppressor genes/proteins and Oncogenes/Proto-oncogenes. Ligand. Growth factors. ... The RING domain also binds specifically to RNA, although the function of this is poorly understood. ...
RNA editing[edit]. The mRNA transcripts of the SDHB gene in human are edited through an unknown mechanism at ORF nucleotide ... Neoplasm: Tumor suppressor genes/proteins and Oncogenes/Proto-oncogenes. Ligand. Growth factors. ... "Hypoxia-inducible C-to-U coding RNA editing downregulates SDHB in monocytes". PeerJ. 1: e152. doi:10.7717/peerj.152. PMC ... "Hypoxia-inducible C-to-U coding RNA editing downregulates SDHB in monocytes". PeerJ. 1: e152. doi:10.7717/peerj.152. PMC ...
Single-stranded RNA replication involves RNA-dependent RNA synthesis which meant that virus-coding enzymes would make partial ... "A Transmissible Avian Neoplasm (Sarcoma of the Common Fowl)". Journal of Experimental Medicine. 12 (5): 696-705. doi:10.1084/ ... RNA Oncoviruses[edit]. Brief history[edit]. In the 1960s, the replication process of RNA virus was believed to be similar to ... RNA viruses[edit]. Not all oncoviruses are DNA viruses. Some RNA viruses have also been associated such as the hepatitis C ...
427 Therapeutic Targeting of an RNA Splicing Factor Network for the Treatment of Myeloid Neoplasms Program: Oral and Poster ... Consistent with this, RNA-sequencing of AML cells following RBM39 deletion revealed significant effects of RBM39 loss on RNA ... We next performed RNA sequencing of isogenic K562 cells with or without knockin of SF3B1K700E and SRSF2P95H mutations into the ... RNA-binding proteins (RBPs) regulate many aspects of transcription and translation in a cell- and tissue-specific manner and ...
Micro-RNAs (miRNAs) belong to a class of small non-coding messenger RNA species that have emerged as potent regulators of a ... exists in the morphological diagnosis of certain types of thyroid carcinomas especially the follicular pattern neoplasm. The ...
What is RNA-Seq?. long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA ... Bias in RNA-seq Library Preparation. *Identifying differentially expressed circular RNAs associated with DNA methylation change ... Expression data from RNA-sequencing can differentiate breast cancer histological grade. May 13, 2016 1 Comment 5,240 Views ... Comparison of RNA-Seq data alignment and qene expression quantification tools for clinical breast cancer research. April 18, ...
RNA sequencing analysis. Five BPDCN cases studied by WES and targeted sequencing had sufficient material for RNA extraction and ... used as an RNA-seq extension set. RNA of 4 normal plasmacytoid dendritic cell (pDCs) samples was purchased from AllCells ( ... RNA-seq) discovery set. We also collected an additional 4 BPDCN cryopreserved cutaneous biopsies, sufficient only for RNA ... Pemmaraju N. Blastic plasmacytoid dendritic cell neoplasm. Clin Adv Hematol Oncol. 2016; 14(4):220-222. Google Scholar ...
RNA sequencing analysis. Five BPDCN cases studied by WES and targeted sequencing had sufficient material for RNA extraction and ... Blastic Plasmacytoid Dendritic Cell Neoplasm (BPDCN) Harbors Frequent Splicesosome Mutations That Cause Aberrant RNA Splicing ... used as an RNA-seq extension set. RNA of 4 normal plasmacytoid dendritic cell (pDCs) samples was purchased from AllCells ( ... RNA-seq) discovery set. We also collected an additional 4 BPDCN cryopreserved cutaneous biopsies, sufficient only for RNA ...
Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. J.H. Matse, J. Yoshizawa, X.Y. Wang, D. Elashoff, J ... Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms. / Matse, J.H.; Yoshizawa, J.; Wang, X.Y.; Elashoff ... title = "Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms", ... T1 - Human Salivary Micro-RNA in Patients with Parotid Salivary Gland Neoplasms ...
RNA isolation and real-time PCR. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) and reverse transcribed ... International consensus guidelines for management of intraductal papillary mucinous neoplasms and mucinous cystic neoplasms of ... Genome-Wide CpG Island Profiling of Intraductal Papillary Mucinous Neoplasms of the Pancreas. Seung-Mo Hong, Noriyuki Omura, ... SOX17 RNA was upregulated in several pancreatic cancer cell lines, including A32-1, Capan1, FamPanc, and Panc2.5, after 5-aza- ...
RNA Sequencing-based Biomarker Characterization of Chromophobe Renal Cell Carcinoma and Related Oncocytic Neoplasms[Formula ... Fingerprint Dive into the research topics of Next-generation RNA Sequencing-based Biomarker Characterization of Chromophobe ... Renal Cell Carcinoma and Related Oncocytic Neoplasms[Formula presented]. Together they form a unique fingerprint. * Sort by ...
Quantification of : RNA. 200. All. 18 Years and older (Adult, Senior). NCT02853006. 38RC15.099. ECTOPIC/MUTAS. June 2015. June ... 442 Studies found for: lung neoplasms AND (woman OR women OR female) ...
Scientists develop new tool to analyse single-cell RNA data in pre-malignant blood cancers Wellcome Trust Sanger Institute ... Neoplasm News and Research. RSS Neoplasm is an abnormal mass of tissue as a result of neoplasia. Further Reading. *Neoplasm - ... Common drug for autoimmune disease may increase risk of myeloid neoplasms Mayo Clinic researchers have found that azathioprine ... infusion for the treatment of blastic plasmacytoid dendritic cell neoplasm in adults and in pediatric patients, two years of ...
... results indicate that high concentration of extracellular DNA in blood plasma along with decreased level of extracellular RNA ... Circulating nucleic acids in blood of patients with stomach and colon neoplasms] Biomed Khim. May-Jun 2005;51(3):321-8. ...
RNA, Neoplasm/analysis*. Substance. *RNA, Neoplasm. Grant support. *Intramural NIH HHS/United States ...
... rearrangement in a myeloid neoplasm with eosinophilia and normal FISH studies.,/p,,/li,,li,,p,Consistent with the patients ... ol,,li,,p,Targeted RNA sequencing detected a cryptic ,i,G3BP1-PDGFRB,/i, ... Targeted RNA sequencing detected a cryptic G3BP1-PDGFRB rearrangement in a myeloid neoplasm with eosinophilia and normal FISH ... A cryptic imatinib-sensitive G3BP1-PDGFRB rearrangement in a myeloid neoplasm with eosinophilia Blood Adv. 2020 Feb 11;4(3):445 ...
RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. The elucidation of the ... Neoplasm Invasiveness. *Neoplasm Metastasis. *Neoplasms. *Neoplasms: classification. *Neoplasms: drug therapy. *Neoplasms: ... RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. The elucidation of the ... Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure ...
Long non-coding RNAs in cancer / edited by Alfons Navarro.. *. Access via Springer for Pitt and UPMC users ...
RNA, Neoplasm. Grant support. *P01 CA094060/CA/NCI NIH HHS/United States ...
View mouse Polr3f Chr2:144527745-144541779 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
J:31495 Abe R, et al., Tissue-specific expression of the gene encoding a mouse RNA binding protein homologous to human HuD ...
The small interfering RNA (siRNA) oligos that directed against the mouse Hmga2 messenger RNA (mRNA, siHmga2) or a scrambled ... The evolving genomic landscape of myeloproliferative neoplasms. Hematology Am Soc Hematol Educ Program. 2014;2014(1):287-296. ... Our findings suggest that, in a subset of myeloproliferative neoplasm patients, the let-7-HMGA2 axis plays a prominent role in ... To decipher the let-7-HMGA2 axis in myeloproliferative neoplasms, we employed an in vitro model supplemented with clinical ...
Blastic plasmacytoid dendritic cell neoplasm: report of a case presenting with lung and central nervous system involvement and ... 8) EBV antigens/EBV-encoded nuclear RNA (EBER) and T-cell/B-cell gene rearrangement were consistently negative. (8-10) The ... MLA style: "Blastic plasmacytoid dendritic cell neoplasm: report of a case presenting with lung and central nervous system ... APA style: Blastic plasmacytoid dendritic cell neoplasm: report of a case presenting with lung and central nervous system ...
Nanomaterials and Neoplasms. Towards Clinical Applications Edited By Valerio Voliani. July 23, 2021 by Jenny Stanford ... Therapeutic RNA Nanotechnology. Immunomodulation and Dynamicity Edited By Kirill A. Afonin. , Morgan Chandler. ...
HPV Anti-CD40 RNA Vaccine. *Carcinoma, Squamous Cell. *Head and Neck Neoplasm ... RNA-Immunotherapy of IVAC_W_bre1_uID and IVAC_M_uID. *Breast Cancer (Triple Negative Breast Cancer (TNBC)) ...
Neoplasms by Histologic Type. Neoplasms. Immune System Diseases. Lentivirus Infections. Retroviridae Infections. RNA Virus ... Neoplasms, Vascular Tissue. Opportunistic Infections. Infection. Parasitic Diseases. Vincristine. Bleomycin. Antineoplastic ...
RNA Virus Infections. Flaviviridae Infections. Adenocarcinoma. Liver Neoplasms. Digestive System Neoplasms. Neoplasms by Site. ... Neoplasms, Glandular and Epithelial. Neoplasms by Histologic Type. Neoplasms. Liver Diseases. Digestive System Diseases. ... HCV RNA and HCV GT must be confirmed by screening lab results) OR b. Positive for anti-HCV antibody or HCV RNA at the time of ... HCV RNA (10,000 IU/mL in peripheral blood) at the time of screening have documented chronic HCV GT1, GT4 (with no evidence of ...
NEOPLASMS; RECEPTORS; RNA. ... RNAs inhibited the ability of TGZ to regulate expression of p21 ...
Lung Neoplasm; Blood; Polymerase Chain Reaction; Cytokeratin; Messenger RNA; Chemotherapy Conflicts of Interest ... Hormonal regulation of prostate-specific antigen messenger RNA in human prostatic adenocarcinoma cell line LNCaP, Cancer Res, ...
Bulk-RNA Sequencing of high-grade pancreatic and non-pancreatic Neuroendocrine Neoplasms ... This dataset entails 40 Bulk-RNA sequenced patient-derived gastro-intestinal neuroendocrine (GEP-NEN) neoplasms. ... This dataset entails 40 Bulk-RNA sequenced patient-derived gastro-intestinal neuroendocrine (GEP-NEN) neoplasms. ... Therapeutic decisions in oncology depend on a precise pathological classification of individual neoplasms. Recent years have ...
RNA accounts for a large proportion in the total noncoding RNAs. The number, type, and function of lncRNA are unclear, so they ... Long noncoding RNA (lncRNA) comprises a group of RNAs that are longer than 200 nucleotides. The lncRNA does not contain ... Long noncoding RNA in digestive system neoplasms / 中国肿瘤临床 ... In this article, the authors summarized the up-to-date research progress on lncRNA in digestive system neoplasms in China and ...
Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens. Website. http://www.dovepress.com/ ... Circular RNA circNELL2 Acts as the Sponge of miR-127-5p to Promote Esophageal Squamous Cell Carcinoma Progression *Xiong G, ... Luciferase assay, RNA pull down and the FISH assay were performed to verify the interaction between circNELL2 and miR-127-5p as ... aberrant circular RNA (circRNA) expression has been researched extensively in the recent times. In the present study, we aim to ...
Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens. Website. http://www.dovepress.com/ ... The Long Noncoding RNA LINC00908 Facilitates Hepatocellular Carcinoma Progression Via Interaction With Sox-4 *Hu X, ... Accumulating evidence has demonstrated a pivotal role of long noncoding RNAs (lncRNAs) in various tumors. However, the function ...
  • Micro-RNAs (miRNAs) belong to a class of small non-coding messenger RNA species that have emerged as potent regulators of a variety of biological processes including oncogenesis. (bmj.com)
  • Adams B, Furneaux H, White B. The micro-ribonucleic acid (miRNA) miR-206 targets the human estrogen receptor-alpha (ERalpha) and represses ERalpha messenger RNA and protein expression in breast cancer cell lines. (labome.org)
  • MicroRNAs (miRNAs) that exert function by posttranscriptional suppression have recently brought insight in our understanding of the role of non-protein-coding RNAs in carcinogenesis and metastasis. (nih.gov)
  • Circular RNAs (circRNAs) have emerged as important regulators in carcinogenesis and metastasis. (medscimonit.com)
  • Effects of small interfering RNA targeting heparanase-1 combined with heparin on invasiveness of mouse hepatocellular carcinoma cell lines. (biomedsearch.com)
  • This study was to explore the effects of specific small interfering RNA (siRNA) targeting HPA-1 combined with heparin on invasiveness of mouse hepatocellular carcinoma cells. (biomedsearch.com)
  • Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and aggressive hematologic malignancy for which there is still no effective therapy. (haematologica.org)
  • The U.S. Food and Drug Administration today approved Elzonris (tagraxofusp-erzs) infusion for the treatment of blastic plasmacytoid dendritic cell neoplasm in adults and in pediatric patients, two years of age and older. (news-medical.net)
  • Blastic plasmacytoid dendritic cell neoplasm: report of a case presenting with lung and central nervous system involvement and review of the literature. (thefreelibrary.com)
  • Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematopoietic malignancy. (thefreelibrary.com)
  • Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare and aggressive malignancy derived from precursors of plasmacytoid dendritic cells. (bloodjournal.org)
  • Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematologic disease, often presenting in the skin. (bloodjournal.org)
  • however, the RNA-Seq data detected additional smoking- and cancer-related transcripts whose expression was were either not interrogated by or was not found to be significantly altered when using microarrays, including smoking-related changes in the inflammatory genes S100A8 and S100A9 and cancer-related changes in MUC5AC and secretoglobin ( SCGB3A1 ). (pubmedcentralcanada.ca)
  • Quantitative real-time PCR confirmed differential expression of select genes and non-coding RNAs within individual samples. (pubmedcentralcanada.ca)
  • Increased RNA expression of genes such as SerpinB10, Wisp2, and Apod in tumors from FO-treated rats is indicative of highly differentiated tumors. (elsevier.com)
  • They are encoded predominantly in introns of host genes in vertebrates, and guide site-specific chemical modifications of ribosomes, transfer RNAs, and small nuclear RNAs. (frontiersin.org)
  • We previously identified genes involved in mitosis or ceramide metabolism that influenced sensitivity to paclitaxel, with an RNA interference (RNAi) screen in three cancer cell lines, including a triple-negative breast-cancer cell line. (dtu.dk)
  • Uma biblioteca lentiviral de 4492 shRNAs ( short hairpin RNAs ), alvejando cerca de 350 genes envolvidos na regulação epigenética, foi empregada para a triagem de genes de suscetibilidade nas células derivadas de PDX, e em outras cinco linhagens tumorais pancreáticas (AsPC-1, BxPC-3, Capan-1, MIA PaCa-2 e PANC-1). (usp.br)
  • RNA-binding proteins (RBPs) regulate many aspects of transcription and translation in a cell- and tissue-specific manner and are frequently dysregulated in malignancy. (confex.com)
  • To explore the mechanism of RBM39 dependence in AML, we performed proteomic analyses of RBM39 interacting proteins in MOLM-13 cells as well as transcriptome-wide analysis of RBM39 RNA binding by enhanced UV cross-linking and immunoprecipitation (eCLIP) in the same cells. (confex.com)
  • Furthermore, TGZ induced the expression of endogenous or exogenous p63 and p73 proteins and p63- or p73-directed short hairpin (si) RNAs inhibited the ability of TGZ to regulate expression of p21 in these cells. (osti.gov)
  • Analysis of truncated WT1 proteins demonstrated that three of four zinc fingers were necessary for RNA-protein interaction. (mendeley.com)
  • The class of non-coding RNAs-molecules that are generally not translated into proteins-are new cancer drivers and suppressors in all types of cancer. (mdpi.com)
  • A wide variety of molecular determinants including transcription factors, epigenetic regulators (e.g., polycomb and trithorax proteins), microRNAs, chromatin structure, and long non-coding RNAs (lncRNAs) are known to control HOX gene expression. (nature.com)
  • To decipher the let-7-HMGA2 axis in myeloproliferative neoplasms, we employed an in vitro model supplemented with clinical correlation. (haematologica.org)
  • In samples from 151 patients with myeloproliferative neoplasms, there was a modest inverse correlation between the expression levels of let-7a and HMGA2 . (haematologica.org)
  • Patients with upregulated HMGA2 showed an increased propensity for developing major thrombotic events, and they were more likely to harbor one of the 3 driver myeloproliferative neoplasm mutations in JAK2 , MPL and CALR . (haematologica.org)
  • The molecular pathophysiology of myeloproliferative neoplasms (MPNs) remains poorly understood. (rupress.org)
  • The Philadelphia-negative myeloproliferative neoplasms (MPNs) constitute a group of closely related hematological disorders that includes polycythemia vera (PV), essential thrombocytosis (ET), and primary myelofibrosis (PMF) and share among other features a propensity to transform to acute leukemia. (rupress.org)
  • In this second part of our two-part review, we discuss the use of mutation profiling in the diagnosis, prognosis, and treatment of patients with myeloproliferative neoplasms and other myeloid diseases. (cancernetwork.com)
  • 1] As an example, for patients with a suspected myeloproliferative neoplasm (MPN), the presence of mutated CALR and/or absence of mutated JAK2 virtually excludes polycythemia vera as a potential diagnosis, with only rare exceptions. (cancernetwork.com)
  • Half of the asxl1 +/ − zebrafish had myeloproliferative neoplasms (MPNs) by 5 months of age. (biologists.org)
  • This video highlights a study that establishes mutated CALR as a myeloproliferative neoplasms-specific tumor antigen and provides a rationale for the development of immunotherapies targeting mutated CALR. (cancernetwork.com)
  • The application of RNA interference (RNAi) to mammalian systems has the potential to revolutionize genetics and produce novel therapies. (ebscohost.com)
  • This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo . (scielo.br)
  • When the expression of CIAPIN1 was downregulated by RNA interference (RNAi) in MCF-7/ADM breast cancer cells, drug resistance was significantly reduced by inhibiting MDR1 expression ( 13 ). (scielo.br)
  • Reinstalling antitumor immunity by inhibiting tumor-derived immunosuppressive molecule IDO through RNA interference. (semanticscholar.org)
  • Induction of RNA interference in dendritic cells. (semanticscholar.org)
  • IDIBELL Researchers of the Neuro-Oncology Unit of Bellvitge University Hospital - Catalan Institute of Oncology, led by Dr. Jordi Bruna, have successfully tested a new molecule capable of preventing the development of peripheral neuropathy induced by chemotherapy in cancer patients, especially in colon cancer cases, the third most common neoplasm in the world. (news-medical.net)
  • Our results indicate that high concentration of extracellular DNA in blood plasma along with decreased level of extracellular RNA on the surface of blood cells correlate with development of gastrointestinal cancer. (nih.gov)
  • 1) Later, the European Organisation for Research and Treatment of Cancer (EORTC) classification of cutaneous lymphoma renamed it as CD4+CD56+ hematodermic neoplasm in 2005. (thefreelibrary.com)
  • Additionally, a growing body of evidence supports the role of long non-coding RNAs (lncRNAs) in cancer development and progression. (mdpi.com)
  • Robert J KleinSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe used RNA sequencing to analyze the genomic signatures of prostate cancer that progressed after targeted alpha therapy. (medworm.com)
  • We hypothesized that transcriptome sequencing (RNA-Seq) will enhance our understanding of the field of molecular injury in response to tobacco smoke exposure and lung cancer pathogenesis by identifying gene expression differences not interrogated or accurately measured by microarrays. (pubmedcentralcanada.ca)
  • The measurement of both coding and non-coding transcripts by RNA-Seq has the potential to help elucidate mechanisms of response to tobacco smoke and to identify additional biomarkers of lung cancer risk and novel targets for chemoprevention. (pubmedcentralcanada.ca)
  • HOXD antisense growth-associated long noncoding RNA (HOXD-AS1) was reported to be upregulated in various cancers, such as gastric cancer, hepatocellular carcinoma, colorectal cancer, and glioma. (dovepress.com)
  • While cell behavior can be modulated by long non-coding RNAs (lncRNAs), the contributions of lncRNAs in progression and berberine effects on colorectal cancer are largely unknown. (medscimonit.com)
  • Second malignant neoplasms are one of the most severe side effects of cancer treatment. (eurekaselect.com)
  • Rejin Kebudi* and Gul Nihal Ozdemir, "Secondary Neoplasms in Children Treated for Cancer", Current Pediatric Reviews (2017) 13: 34. (eurekaselect.com)
  • This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. (google.com.au)
  • Further, this invention allows the qualitative or quantitative detection of tumor-derived or associated extracellular RNA circulating in the plasma or serum of humans or animals with or without any prior knowledge of the presence of cancer or premalignant tissue. (google.com.au)
  • Long Noncoding RNA and Cancer: A New Paradigm. (harvard.edu)
  • Encapsulated forms of the follicular variant with the absence of certain adverse features has led to another variant termed noninvasive follicular neoplasm with papillary-like nuclear features (NIFTP), which is currently considered to not be a cancer. (thyroid.org)
  • Pancreatic ductal adenocarcinoma (PDAC), the most prevalent type of pancreatic cancer, is a highly aggressive and lethal neoplasm. (usp.br)
  • Folate is necessary for the production and maintenance of new cells , for DNA synthesis and RNA synthesis through methylation , and for preventing changes to DNA , thus for preventing cancer . (wikipedia.org)
  • These findings provide the first evidence for the posttranscriptional regulation of ERalpha by a micro-RNA in the context of breast cancer. (labome.org)
  • Compositions and methods for expanding CD34+ cells, performing research related to cancer stem cells, RNA-editing enzymes and for monitoring, diagnosing and treating, ameliorating and preventing diseases such as cancers or inflammatory diseases. (patents.com)
  • Long noncoding RNA ( lncRNA ) comprises a group of RNAs that are longer than 200 nucleotides . (bvsalud.org)
  • In this article, the authors summarized the up-to-date research progress on lncRNA in digestive system neoplasms in China and overseas to provide new insights into the genetic diagnosis and treat-ment of digestive system neoplasms in clinics. (bvsalud.org)
  • Here, we show that the activation of HOXBLINC , a HOXB locus-associated long non-coding RNA (lncRNA), is a critical downstream mediator of NPM1c + -associated leukemic transcription program and leukemogenesis. (nature.com)
  • Recently, long noncoding RNAs (lncRNA) have emerged as new gene regulators and prognostic markers in several cancers, including renal cell carcinoma (RCC). (aacrjournals.org)
  • Based on size, ncRNAs are divided into two groups, small noncoding RNAs and long noncoding RNAs (lncRNA) greater than 200 nt. (aacrjournals.org)
  • RNA immunoprecipitation (RIP) assay was done to verify the interaction between lncRNA CASC2 and (AU-binding factor 1) AUF1, or AUF1 and B-cell CLL/lymphoma 2 (Bcl-2). (medscimonit.com)
  • Accumulating evidence has demonstrated a pivotal role of long noncoding RNAs (lncRNAs) in various tumors. (doaj.org)
  • To determine if these genetic lesions appear in Drosophila tumors we have sequenced the genomes of 17 malignant neoplasms caused by mutations in l(3)mbt , brat , aurA , or lgl . (g3journal.org)
  • Many of these tumors are hyperplasias that present during larval development and eventually differentiate, but others behave as frankly malignant neoplasms that are refractory to differentiation signals, lethal to the host and immortal. (g3journal.org)
  • The question remains, however, as to the extent of GI in other samples of Ph tumors and, indeed, in different types of Drosophila malignant neoplasms. (g3journal.org)
  • Steroid levels were not influenced by their corresponding receptor expression in mammary neoplasms, but increased PRL levels were negatively associated with low PRLR gene expression in malignant tumors. (biomedcentral.com)
  • Hierarchical clustering analyses on these RNAs and their predicted mRNA targets showed that the majority of differentially expressed circRNAs and mRNAs had been up-regulated in the bladder tumors. (medscimonit.com)
  • Molecular studies of lymphoblastic B-cell lymphoma, the most prevalent neoplasm (50% of all tumors), showed that the lymphomas were clonal, overexpressed Myc His , and exhibited the P2 to P1 promoter shift in Myc expression, a hallmark of MYC/Myc deregulation in human endemic Burkitt lymphoma and mouse plasmacytoma. (aacrjournals.org)
  • Intraductal papillary mucinous neoplasms(IPMN) are common cystic tumors of the pancreas and are known to show transition from adenoma to carcinoma accompanied by several molecular abnormalities. (nii.ac.jp)
  • In other studies, increased levels of estrogens, progesterone and prolactin were found in serum and/or tissue homogenates of dogs with malignant neoplasms. (biomedcentral.com)
  • Herein, it has been confirmed that transformation towards malignant neoplasms is associated with significant reduction of gene expression of particular hormone receptors. (biomedcentral.com)
  • Thus, homologous MYC/Myc -Eμ rearrangements lead to B-cell and plasma-cell neoplasms in humans and mice. (aacrjournals.org)
  • Although a number of studies have characterized the DNA binding properties of the WT1 protein, recent evidence has suggested that WT1 may also have a role in RNA metabolism. (mendeley.com)
  • In line with this, a recent systematic review of available data on the development of mammary neoplasms revealed weak evidence for risk reduction after neutering and an effect of age at neutering. (biomedcentral.com)
  • Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS. (labome.org)
  • The second aspect covered by this review is that of a rather novel category of RNAs, the microRNAs which, although non-coding, functionally behave as oncogenes or tumor suppressors through the negative control they exert on conventional oncogenes or suppressors. (cnrs.fr)
  • MicroRNAs (miRNAs) are a group of small RNAs with a fundamental role in the regulation of gene expression. (hindawi.com)
  • MicroRNAs (miRNAs), which are endogenous small noncoding RNAs consisting of 20-25 nucleotides, may regulate as much as 60% of the human genome [ 6 ]. (hindawi.com)
  • Intraductal papillary mucinous neoplasms (IPMN) are precursors to infiltrating pancreatic ductal adenocarcinomas. (aacrjournals.org)
  • Intraductal papillary mucinous neoplasms (IPMN) are an important precursor to pancreatic adenocarcinoma. (aacrjournals.org)
  • Intraductal papillary mucinous neoplasms (IPMN) arise in the pancreatic ductal system and are distinct mucin-producing cystic precursor lesions of pancreatic ductal adenocarcinomas. (aacrjournals.org)
  • A fim de aproveitar esse recurso para a investigação de potenciais alvos terapêuticos no PDAC, um rastreamento de vulnerabilidades moleculares foi realizado por meio de silenciamento gênico em larga-escala com RNA de interferência (RNAi). (usp.br)
  • Mutation discovery and characterization in lymphoid neoplasms using massively parallel RNA and DNA sequencing. (bcgsc.ca)
  • Consistent with this, RNA-sequencing of AML cells following RBM39 deletion revealed significant effects of RBM39 loss on RNA splicing, most prominently causing increased cassette exon skipping ( Fig. F ). (confex.com)
  • Targeted RNA sequencing detected a cryptic G3BP1-PDGFRB rearrangement in a myeloid neoplasm with eosinophilia and normal FISH studies. (nih.gov)
  • With the advance of the next-generation sequencing technologies, RNA-seq has become a useful tool in defining the transcriptomes of cells with the advantage of analyzing expression at exon levels as well as delineating novel splicing variants [10] , [11] . (plos.org)
  • Ian D McGilvraySeries Type : Expression profiling by high throughput sequencingOrganism : Marmota monaxWe characterized the identity of hepatic macrophages from the uninvolved liver of a WHV-positive (area with no visible tumor) tumor bearing woodchuck that take up NPs using RNA-sequencing (RNA-seq). (medworm.com)
  • Patients and Methods: 2239 patients with hematological neoplasms (Table1) were evaluated by whole transcriptome sequencing (WTS) and 64 cases without hematological neoplasms were analysed as controls. (bloodjournal.org)
  • RNA-Seq libraries were prepared using 2 distinct approaches, one capable of capturing non-polyadenylated RNA (the prototype NuGEN Ovation RNA-Seq protocol) and the other designed to measure only polyadenylated RNA (the standard Illumina mRNA-Seq protocol) followed by sequencing generating approximately 29 million 36 nt reads per pool and approximately 22 million 75 nt paired-end reads per pool, respectively. (pubmedcentralcanada.ca)
  • Transcriptome sequencing (RNA-Seq) is a recently developed technology that uses high-throughput sequencing approaches (next-generation sequencing or NGS) to determine the sequence of all RNA transcripts in a given specimen. (springer.com)
  • Ozsolak F, Milos PM. RNA sequencing: advances, challenges and opportunities. (springer.com)
  • Could Single-Cell RNA Sequencing Help Personalize Multiple Myeloma Treatment? (cancernetwork.com)
  • This video highlights research that found that single-cell RNA sequencing can better characterize patients and potentially improve multiple myeloma treatment in a more personalized manner. (cancernetwork.com)
  • In the present study, we describe a novel strategy to disrupt tumor-derived immune suppression by silencing a tolerogenic molecule of tumor origin, IDO, using small interfering RNA (siRNA). (semanticscholar.org)
  • A neoplasm or tumor is a group of cells that have undergone unregulated growth and will often form a mass or lump, but may be distributed diffusely. (wikipedia.org)
  • RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. (mendeley.com)
  • RNA transcription and subsequent protein synthesis are less affected by folate deficiency, as the mRNA can be recycled and used again (as opposed to DNA synthesis, where a new genomic copy must be created). (wikipedia.org)
  • Therapeutic decisions in oncology depend on a precise pathological classification of individual neoplasms. (ega-archive.org)
  • To date, RNA-seq analysis of HBV-related hepatocellular carcinoma (HCC) has not been reported. (plos.org)
  • Aberrant expression of several miRNAs was found to be involved in a large variety of neoplasms, including hepatocellular carcinoma (HCC). (hindawi.com)
  • Researchers have evaluated the presence of intratumoral vascularization in ovarian neoplasms by Color-flow Doppler ultrasound. (knowcancer.com)
  • The aim of this review is to give an overview of the current knowledge regarding the role of non-coding RNAs (including short and long non-coding RNAs) in non-ccRCC and to highlight possible implications as diagnostic, prognostic and predictive biomarkers. (mdpi.com)
  • Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIR. (harvard.edu)
  • Background: Resistance to apoptosis is one of the hallmarks in hematological neoplasms, most typically via dysregulation of the intrinsic mitochondrial pathway. (bloodjournal.org)
  • Aim: To determine the gene expression levels of BCL2 , BCL2L1 and MCL1 across various hematological neoplasms in order to identify best candidates for pro-survival protein inhibitors. (bloodjournal.org)
  • Beginning at the age of 6 months, Myc His transgenic mice developed B-cell and plasma neoplasms, such as IgM + lymphoblastic B-cell lymphomas, Bcl-6 + diffuse large B-cell lymphomas, and CD138 + plasmacytomas, with an overall incidence of 68% by 21 months. (aacrjournals.org)
  • We also review research and potential clinical applications of RNA-Seq technology in inherited, chronic, neoplastic, and infectious diseases. (springer.com)
  • The quantitative analyses of MUC1, MUC5AC, S 100P, S100A11 and SHH are useful for differentiation between neoplasms and non-neoplastic lesions. (nii.ac.jp)
  • The elucidation of the mechanisms by which these modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. (mendeley.com)
  • What 3 identical but functionally different RNA molecules are transcribed from anti-sense (-) DNA (template strand)? (brainscape.com)
  • Small nucleolar RNAs (snoRNAs) are a class of small non-coding RNA molecules, 60-300 base pairs in length. (frontiersin.org)
  • Studies have shown that small nucleolar RNAs (snoRNAs) play important roles in several cancers. (frontiersin.org)
  • Neoplasms of the mammary gland are among the most common diseases in female domestic dogs ( Canis familiaris ). (biomedcentral.com)
  • Owing to its involvement in both the initiation and progression of various cancers, aberrant circular RNA (circRNA) expression has been researched extensively in the recent times. (doaj.org)
  • Thermo Fisher Scientific's Ion Torrent Oncomine Myeloid Research Assay interrogates DNA mutations, RNA fusion transcripts and gene expression levels associated with myeloid cancers in a single run, yielding results in 2-3 days. (medindia.net)
  • Overall, to our best knowledge, our study represents the most comprehensive characterization of HBV-related HCC transcriptome including exon level expression changes and novel splicing variants, which illustrated the power of RNA-seq and provided important clues for understanding the molecular mechanisms of HCC pathogenesis at system-wide levels. (plos.org)
  • Marioni JC, Mason CE, Mane SM, Stephens M, Gilad Y. RNA-seq: an assessment of technical reproducibility and comparison with gene expression arrays. (springer.com)
  • The aim of this review is to draw the attention to the numerous steps of gene expression which operate at the RNA level and which are significant drivers of the transformation process. (cnrs.fr)
  • Small noncoding RNAs, such as miRNAs, have been studied extensively, and their roles in gene regulation and cell function have been elucidated in numerous cancers ( 7 ). (aacrjournals.org)
  • long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. (rna-seqblog.com)
  • c) detecting in a quantitative or qualitative fashion one or more amplified RNA or cDNA products or signals associated with a disease with or without comparison to RNA not associated with a disease, whereby a disease in a human is characterized or evaluated without performance of a biopsy or surgery. (google.com.au)
  • 4 . The method of claim 1 , wherein cDNA is produced by reverse transcription of RNA. (google.com.au)
  • l) a combination or variation thereof that amplifies or signal amplifies the RNA or cDNA. (google.com.au)
  • Additionally, the deconvolution results were clinically interpretable and further research can render the deconvolution approach an effective complementary asset for the clinical classification of neoplasms. (ega-archive.org)
  • Therapy-related myeloid neoplasm (t-MN) is a distinctive clinical syndrome occurring after exposure to chemotherapy or radiotherapy. (ebscohost.com)
  • Identification of two distinct molecular subtypes of non-invasive follicular neoplasm with papillarylike nuclear features by digital RNA counting. (thyroid.org)