A species of gram-negative bacteria and an nitrogen inoculum that displays a high intrinsic tolerance to acidity.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
A plant genus in the family FABACEAE which is the source of edible beans and the lectin PHYTOHEMAGGLUTININS.
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.
A species of gram-negative, aerobic bacteria that is found in soil and which causes formation of root nodules on some, but not all, types of field pea, lentil, kidney bean, and clover.
Beneficial microorganisms (bacteria or fungi) encapsulated in carrier material and applied to the environment for remediation and enhancement of agricultural productivity.
A genus of gram-negative, aerobic, nonsporeforming rods which usually contain granules of poly-beta-hydroxybutyrate. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.
Knobbed structures formed from and attached to plant roots, especially of LEGUMES, which result from symbiotic infection by nitrogen fixing bacteria such as RHIZOBIUM or FRANKIA. Root nodules are structures related to MYCORRHIZAE formed by symbiotic associations with fungi.
A species of gram-negative bacteria and nitrogen innoculant of PHASEOLUS VULGARIS.
The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.
The functional hereditary units of BACTERIA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
Proteins found in any species of bacterium.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.
The formation of a nitrogen-fixing cell mass on PLANT ROOTS following symbiotic infection by nitrogen-fixing bacteria such as RHIZOBIUM or FRANKIA.
The relationships of groups of organisms as reflected by their genetic makeup.
A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.

GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the Sinorhizobium meliloti-alfalfa symbiotic interaction. (1/20)

The guaB mutant strain Rhizobium tropici CIAT8999-10T is defective in symbiosis with common bean, forming nodules that lack rhizobial content. In order to investigate the timing of the guaB requirement during the nodule formation on the host common bean by the strain CIAT899-10.T, we constructed gene fusions in which the guaB gene is expressed under the control of the symbiotic promoters nodA, bacA, and nifH. Our data indicated that the guaB is required from the early stages of nodulation because full recovery of the wild-type phenotype was accomplished by the nodA-guaB fusion. In addition, we have constructed a guaB mutant derived from Sinorhizobium meliloti 1021, and shown that, unlike R. tropici, the guaB S. meliloti mutant is auxotrophic for guanine and induces wild-type nodules on alfalfa and Medicago truncatula. The guaB R. tropici mutant also is defective in its symbiosis with Macroptilium atropurpureum and Vigna unguiculata but normal with Leucaena leucocephala. These results show that the requirement of the rhizobial guaB for symbiosis is found to be associated with host plants that form determinate type of nodules.  (+info)

An abundance of nodulation factors. (2/20)

In this issue of Chemistry & Biology, Moron et al. [1] report that Rhizobium tropici CIAT899 produces different Nod factors in response to flavonoid induction under differing environmental conditions. This unanticipated environmental dependence has implications for altering or potentially improving the host-bacteria interaction in bean nodulation.  (+info)

Low pH changes the profile of nodulation factors produced by Rhizobium tropici CIAT899. (3/20)

Rhizobium tropici CIAT899 has been cataloged as a nodulator of bean, a plant often growing in areas characterized by highly acidic soils. The purpose of this work was to explore the effects of acidity on the production of Nod factors by this strain and their impact on the establishment of effective symbioses. We report that acidity increases rhizobial Nod factors production, and we exhaustively study the nodulation factor structures produced under abiotic stress. Significant differences were observed between the structures produced at acid and neutral pH: 52 different molecules were produced at acid pH, 29 at neutral pH, and only 15 are common to bacteria grown at pH 7.0 or 4.5. The results indicate that R. tropici CIAT899 has successfully adapted to life in acidic soils and is a good inoculant for the bean under these conditions.  (+info)

A ClC chloride channel homolog and ornithine-containing membrane lipids of Rhizobium tropici CIAT899 are involved in symbiotic efficiency and acid tolerance. (4/20)

Rhizobium tropici CIAT899 is highly tolerant to several environmental stresses and is a good competitor for nodule occupancy of common bean plants in acid soils. Random transposon mutagenesis was performed to identify novel genes of this strain involved in symbiosis and stress tolerance. Here, we present a genetic analysis of the locus disrupted by the Tn5 insertion in mutant 899-PV9, which lead to the discovery of sycA, a homolog of the ClC family of chloride channels and Cl-/H+ exchange transporters. A nonpolar deletion in this gene caused serious deficiencies in nodule development, nodulation competitiveness, and N2 fixation on Phaseolus vulgaris plants, probably due to its reduced ability to invade plant cells and to form stable symbiosomes, as judged by electron transmission microscopy. A second gene (olsC), found downstream of sycA, is homologous to aspartyl/asparaginyl beta-hydroxylases and modifies two species of ornithine-containing lipids in vivo, presumably by hydroxylation at a still-unknown position. A mutant carrying a nonpolar deletion in olsC is symbiotically defective, whereas overexpressed OlsC in the complemented strain provokes an acid-sensitive phenotype. This is the first report of a ClC homolog being essential for the establishment of a fully developed N2-fixing root nodule symbiosis and of a putative beta-hydroxylase that modifies ornithine-containing membrane lipids of R. tropici CIAT899, which, in turn, are contributing to symbiotic performance and acid tolerance.  (+info)

Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics. (5/20)

A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and beta-glucuronidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root-microbe interactions.  (+info)

Rhizobium tropici response to acidity involves activation of glutathione synthesis. (6/20)

Rhizobium tropici CIAT899 displays intrinsic tolerance to acidity, and efficiently nodulates Phaseolus vulgaris at low pH. By characterizing a gshB mutant strain, glutathione has been previously demonstrated to be essential for R. tropici tolerance to acid stress. The wild-type gshB gene region has been cloned and its transcription profile has been characterized by using quantitative real-time PCR and transcriptional gene fusions. Activation of the gshB gene under acid-stress conditions was demonstrated. gshB is also induced by UV irradiation. Upstream from gshB a putative sigma(70) promoter element and an inverted repeat sequence were identified, which are proposed to be involved in expression under neutral and acidic conditions, respectively. Gel retardation assays indicate that transcription in acid conditions may involve protein binding to an upstream regulatory region.  (+info)

The lipid lysyl-phosphatidylglycerol is present in membranes of Rhizobium tropici CIAT899 and confers increased resistance to polymyxin B under acidic growth conditions. (7/20)

Lysyl-phosphatidylglycerol (LPG) is a well-known membrane lipid in several gram-positive bacteria but is almost unheard of in gram-negative bacteria. In Staphylococcus aureus, the gene product of mprF is responsible for LPG formation. Low pH-inducible genes, termed IpiA, have been identified in the gram-negative alpha-proteobacteria Rhizobium tropici and Sinorhizobium medicae in screens for acid-sensitive mutants and they encode homologs of MprF. An analysis of the sequenced bacterial genomes reveals that genes coding for homologs of MprF from S. aureus are present in several classes of organisms throughout the bacterial kingdom. In this study, we show that the expression of lpiA from R. tropici in the heterologous hosts Escherichia coli and Sinorhizobium meliloti causes formation of LPG. A wild-type strain of R. tropici forms LPG (about 1% of the total lipids) when the cells are grown in minimal medium at pH 4.5 but not when grown in minimal medium at neutral pH or in complex tryptone yeast (TY) medium at either pH. LPG biosynthesis does not occur when lpiA is deleted and is restored upon complementation of lpiA-deficient mutants with a functional copy of the lpiA gene. When grown in the low-pH medium, lpiA-deficient rhizobial mutants are over four times more susceptible to the cationic peptide polymyxin B than the wild type.  (+info)

An alternative succinate (2-oxoglutarate) transport system in Rhizobium tropici is induced in nodules of Phaseolus vulgaris. (8/20)

 (+info)

Rhizobium tropici is a gram-negative, aerobic, motile, non-spore forming bacteria that belongs to the Rhizobiaceae family. It has the ability to fix atmospheric nitrogen in a symbiotic relationship with certain leguminous plants, particularly beans and other tropical legumes. The bacterium infects the roots of these plants and forms nodules where it converts nitrogen gas into ammonia, which is then used by the plant for growth. Rhizobium tropici is known for its ability to survive in a wide range of temperatures and soil conditions, making it an important contributor to sustainable agriculture in tropical regions.

Rhizobium is not a medical term, but rather a term used in microbiology and agriculture. It refers to a genus of gram-negative bacteria that can fix nitrogen from the atmosphere into ammonia, which can then be used by plants as a nutrient. These bacteria live in the root nodules of leguminous plants (such as beans, peas, and clover) and form a symbiotic relationship with them.

The host plant provides Rhizobium with carbon sources and a protected environment within the root nodule, while the bacteria provide the plant with fixed nitrogen. This mutualistic interaction plays a crucial role in maintaining soil fertility and promoting plant growth.

While Rhizobium itself is not directly related to human health or medicine, understanding its symbiotic relationship with plants can have implications for agricultural practices, sustainable farming, and global food security.

"Phaseolus" is a term that refers to a genus of plants in the legume family Fabaceae, also known as the pea family. The most common and well-known species in this genus is "Phaseolus vulgaris," which is commonly called the common bean. This includes many familiar varieties such as kidney beans, black beans, navy beans, pinto beans, and green beans.

These plants are native to the Americas and have been cultivated for thousands of years for their edible seeds (beans) and pods (green beans). They are an important source of protein, fiber, vitamins, and minerals in many diets around the world.

It's worth noting that "Phaseolus" is a taxonomic term used in the scientific classification of plants, and it does not have a specific medical definition. However, the beans from these plants do have various health benefits and potential medicinal properties, such as being associated with reduced risk of heart disease, improved gut health, and better blood sugar control.

Fabaceae is the scientific name for a family of flowering plants commonly known as the legume, pea, or bean family. This family includes a wide variety of plants that are important economically, agriculturally, and ecologically. Many members of Fabaceae have compound leaves and produce fruits that are legumes, which are long, thin pods that contain seeds. Some well-known examples of plants in this family include beans, peas, lentils, peanuts, clover, and alfalfa.

In addition to their importance as food crops, many Fabaceae species have the ability to fix nitrogen from the atmosphere into the soil through a symbiotic relationship with bacteria that live in nodules on their roots. This makes them valuable for improving soil fertility and is one reason why they are often used in crop rotation and as cover crops.

It's worth noting that Fabaceae is sometimes still referred to by its older scientific name, Leguminosae.

Medicinal plants are defined as those plants that contain naturally occurring chemical compounds which can be used for therapeutic purposes, either directly or indirectly. These plants have been used for centuries in various traditional systems of medicine, such as Ayurveda, Chinese medicine, and Native American medicine, to prevent or treat various health conditions.

Medicinal plants contain a wide variety of bioactive compounds, including alkaloids, flavonoids, tannins, terpenes, and saponins, among others. These compounds have been found to possess various pharmacological properties, such as anti-inflammatory, analgesic, antimicrobial, antioxidant, and anticancer activities.

Medicinal plants can be used in various forms, including whole plant material, extracts, essential oils, and isolated compounds. They can be administered through different routes, such as oral, topical, or respiratory, depending on the desired therapeutic effect.

It is important to note that while medicinal plants have been used safely and effectively for centuries, they should be used with caution and under the guidance of a healthcare professional. Some medicinal plants can interact with prescription medications or have adverse effects if used inappropriately.

Nitrogen fixation is a process by which nitrogen gas (N2) in the air is converted into ammonia (NH3) or other chemically reactive forms, making it available to plants and other organisms for use as a nutrient. This process is essential for the nitrogen cycle and for the growth of many types of plants, as most plants cannot utilize nitrogen gas directly from the air.

In the medical field, nitrogen fixation is not a commonly used term. However, in the context of microbiology and infectious diseases, some bacteria are capable of fixing nitrogen and this ability can contribute to their pathogenicity. For example, certain species of bacteria that colonize the human body, such as those found in the gut or on the skin, may be able to fix nitrogen and use it for their own growth and survival. In some cases, these bacteria may also release fixed nitrogen into the environment, which can have implications for the ecology and health of the host and surrounding ecosystems.

'Rhizobium leguminosarum' is a species of bacteria that can form nitrogen-fixing nodules on the roots of certain leguminous plants, such as clover, peas, and beans. These bacteria have the ability to convert atmospheric nitrogen into ammonia, a form of nitrogen that plants can use for growth. This process, known as biological nitrogen fixation, benefits both the bacteria and the host plant, as the plant provides carbon sources to the bacteria, while the bacteria provide fixed nitrogen to the plant. The formation of this symbiotic relationship is facilitated by a molecular signaling process between the bacterium and the plant.

It's important to note that 'Rhizobium leguminosarum' is not a medical term per se, but rather a term used in microbiology, botany, and agriculture.

Agricultural inoculants are biological products that contain beneficial microorganisms, such as bacteria or fungi, which are applied to seeds, soil, or plant surfaces to enhance plant growth, increase yield, and improve resistance to pests and diseases. These microorganisms form a mutually beneficial relationship with the plants, known as symbiosis, in which they help the plants absorb nutrients from the soil, fix nitrogen, and produce phytohormones that promote root growth and development.

Examples of agricultural inoculants include Rhizobia bacteria, which form nodules on the roots of leguminous plants and convert atmospheric nitrogen into ammonia that can be used by the plant; mycorrhizal fungi, which colonize plant roots and help them absorb water and nutrients from the soil; and Trichoderma fungi, which protect plants from pathogens and promote growth.

Agricultural inoculants are considered an environmentally friendly and sustainable alternative to chemical fertilizers and pesticides, as they improve crop productivity while reducing the negative impacts on the environment and human health.

"Sinorhizobium" is a genus of bacteria that can form nitrogen-fixing nodules on the roots of certain leguminous plants, such as beans and alfalfa. These bacteria are able to convert atmospheric nitrogen into ammonia, which the plant can then use for growth. This symbiotic relationship benefits both the plant and the bacteria - the plant receives a source of nitrogen, while the bacteria receive carbon and other nutrients from the plant.

The genus "Sinorhizobium" is part of the family Rhizobiaceae and includes several species that are important for agriculture and the global nitrogen cycle. Some examples of "Sinorhizobium" species include S. meliloti, which forms nodules on alfalfa and other Medicago species, and S. fredii, which forms nodules on soybeans and other Glycine species.

It's worth noting that the taxonomy of nitrogen-fixing bacteria has undergone significant revisions in recent years, and some "Sinorhizobium" species have been reclassified as members of other genera. However, the genus "Sinorhizobium" remains a valid and important group of nitrogen-fixing bacteria.

Rhizobiaceae is a family of bacteria that have the ability to fix nitrogen. These bacteria are gram-negative, motile, and rod-shaped. They are commonly found in the root nodules of leguminous plants, where they form a symbiotic relationship with the plant. The bacteria provide the plant with fixed nitrogen, while the plant provides the bacteria with carbon and a protected environment.

The most well-known genus of Rhizobiaceae is Rhizobium, which includes several species that are important for agriculture because of their ability to fix nitrogen in the root nodules of legumes. Other genera in this family include Bradyrhizobium, Mesorhizobium, and Sinorhizobium.

It's worth noting that while Rhizobiaceae bacteria are generally beneficial, they can sometimes cause disease in plants under certain conditions. For example, some strains of Rhizobium can cause leaf spots on certain crops.

Root nodules in plants refer to the specialized structures formed through the symbiotic relationship between certain leguminous plants and nitrogen-fixing bacteria, most commonly belonging to the genus Rhizobia. These nodules typically develop on the roots of the host plant, providing an ideal environment for the bacteria to convert atmospheric nitrogen into ammonia, a form that can be directly utilized by the plant for growth and development.

The formation of root nodules begins with the infection of the plant's root hair cells by Rhizobia bacteria. This interaction triggers a series of molecular signals leading to the differentiation of root cortical cells into nodule primordia, which eventually develop into mature nodules. The nitrogen-fixing bacteria reside within these nodules in membrane-bound compartments called symbiosomes, where they reduce atmospheric nitrogen into ammonia through an enzyme called nitrogenase.

The plant, in turn, provides the bacteria with carbon sources and other essential nutrients required for their growth and survival within the nodules. The fixed nitrogen is then transported from the root nodules to other parts of the plant, enhancing its overall nitrogen nutrition and promoting sustainable growth without the need for external nitrogen fertilizers.

In summary, root nodules in plants are essential structures formed through symbiotic associations with nitrogen-fixing bacteria, allowing leguminous plants to convert atmospheric nitrogen into a usable form while also benefiting the environment by reducing the reliance on chemical nitrogen fertilizers.

Rhizobium etli is a gram-negative, aerobic, motile, non-spore forming bacteria that belongs to the Rhizobiaceae family. It has the ability to fix atmospheric nitrogen in a symbiotic relationship with certain leguminous plants, particularly common bean (Phaseolus vulgaris). This bacterium infects the roots of these plants and forms nodules where it converts nitrogen gas into ammonia, a form that can be used by the plant for growth. The nitrogen-fixing ability of Rhizobium etli makes it an important bacteria in agriculture and environmental science.

In the context of medicine and biology, symbiosis is a type of close and long-term biological interaction between two different biological organisms. Generally, one organism, called the symbiont, lives inside or on another organism, called the host. This interaction can be mutually beneficial (mutualistic), harmful to the host organism (parasitic), or have no effect on either organism (commensal).

Examples of mutualistic symbiotic relationships in humans include the bacteria that live in our gut and help us digest food, as well as the algae that live inside corals and provide them with nutrients. Parasitic symbioses, on the other hand, involve organisms like viruses or parasitic worms that live inside a host and cause harm to it.

It's worth noting that while the term "symbiosis" is often used in popular culture to refer to any close relationship between two organisms, in scientific contexts it has a more specific meaning related to long-term biological interactions.

A bacterial gene is a segment of DNA (or RNA in some viruses) that contains the genetic information necessary for the synthesis of a functional bacterial protein or RNA molecule. These genes are responsible for encoding various characteristics and functions of bacteria such as metabolism, reproduction, and resistance to antibiotics. They can be transmitted between bacteria through horizontal gene transfer mechanisms like conjugation, transformation, and transduction. Bacterial genes are often organized into operons, which are clusters of genes that are transcribed together as a single mRNA molecule.

It's important to note that the term "bacterial gene" is used to describe genetic elements found in bacteria, but not all genetic elements in bacteria are considered genes. For example, some DNA sequences may not encode functional products and are therefore not considered genes. Additionally, some bacterial genes may be plasmid-borne or phage-borne, rather than being located on the bacterial chromosome.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

Bacterial DNA refers to the genetic material found in bacteria. It is composed of a double-stranded helix containing four nucleotide bases - adenine (A), thymine (T), guanine (G), and cytosine (C) - that are linked together by phosphodiester bonds. The sequence of these bases in the DNA molecule carries the genetic information necessary for the growth, development, and reproduction of bacteria.

Bacterial DNA is circular in most bacterial species, although some have linear chromosomes. In addition to the main chromosome, many bacteria also contain small circular pieces of DNA called plasmids that can carry additional genes and provide resistance to antibiotics or other environmental stressors.

Unlike eukaryotic cells, which have their DNA enclosed within a nucleus, bacterial DNA is present in the cytoplasm of the cell, where it is in direct contact with the cell's metabolic machinery. This allows for rapid gene expression and regulation in response to changing environmental conditions.

A plant root is not a medical term per se, but it is a term from botany, which is the study of plants. However, I can certainly provide a definition for you.

Plant roots are the underground organs of a plant that typically grow downward into the soil. They serve several important functions, including:

1. Anchorage: Roots help to stabilize the plant and keep it upright in the ground.
2. Absorption: Roots absorb water and nutrients from the soil, which are essential for the plant's growth and development.
3. Conduction: Roots conduct water and nutrients up to the above-ground parts of the plant, such as the stem and leaves.
4. Vegetative reproduction: Some plants can reproduce vegetatively through their roots, producing new plants from root fragments or specialized structures called rhizomes or tubers.

Roots are composed of several different tissues, including the epidermis, cortex, endodermis, and vascular tissue. The epidermis is the outermost layer of the root, which secretes a waxy substance called suberin that helps to prevent water loss. The cortex is the middle layer of the root, which contains cells that store carbohydrates and other nutrients. The endodermis is a thin layer of cells that surrounds the vascular tissue and regulates the movement of water and solutes into and out of the root. The vascular tissue consists of xylem and phloem, which transport water and nutrients throughout the plant.

Bacterial proteins are a type of protein that are produced by bacteria as part of their structural or functional components. These proteins can be involved in various cellular processes, such as metabolism, DNA replication, transcription, and translation. They can also play a role in bacterial pathogenesis, helping the bacteria to evade the host's immune system, acquire nutrients, and multiply within the host.

Bacterial proteins can be classified into different categories based on their function, such as:

1. Enzymes: Proteins that catalyze chemical reactions in the bacterial cell.
2. Structural proteins: Proteins that provide structural support and maintain the shape of the bacterial cell.
3. Signaling proteins: Proteins that help bacteria to communicate with each other and coordinate their behavior.
4. Transport proteins: Proteins that facilitate the movement of molecules across the bacterial cell membrane.
5. Toxins: Proteins that are produced by pathogenic bacteria to damage host cells and promote infection.
6. Surface proteins: Proteins that are located on the surface of the bacterial cell and interact with the environment or host cells.

Understanding the structure and function of bacterial proteins is important for developing new antibiotics, vaccines, and other therapeutic strategies to combat bacterial infections.

Ribosomal RNA (rRNA) is a type of RNA that combines with proteins to form ribosomes, which are complex structures inside cells where protein synthesis occurs. The "16S" refers to the sedimentation coefficient of the rRNA molecule, which is a measure of its size and shape. In particular, 16S rRNA is a component of the smaller subunit of the prokaryotic ribosome (found in bacteria and archaea), and is often used as a molecular marker for identifying and classifying these organisms due to its relative stability and conservation among species. The sequence of 16S rRNA can be compared across different species to determine their evolutionary relationships and taxonomic positions.

"Sinorhizobium meliloti" is a species of nitrogen-fixing bacteria that forms nodules on the roots of leguminous plants, such as alfalfa and clover. These bacteria have the ability to convert atmospheric nitrogen into ammonia, which can then be used by the plant for growth and development. This symbiotic relationship benefits both the bacterium and the plant, as the plant provides carbon sources to the bacterium, while the bacterium provides the plant with a source of nitrogen.

"Sinorhizobium meliloti" is gram-negative, motile, and rod-shaped, and it can be found in soil and root nodules of leguminous plants. It has a complex genome consisting of a circular chromosome and several plasmids, which carry genes involved in nitrogen fixation and other important functions. The bacteria are able to sense and respond to various environmental signals, allowing them to adapt to changing conditions and establish successful symbioses with their host plants.

In addition to its agricultural importance, "Sinorhizobium meliloti" is also a model organism for studying the molecular mechanisms of symbiotic nitrogen fixation and bacterial genetics.

Plant root nodulation is a type of symbiotic relationship between certain plants (mostly legumes) and nitrogen-fixing bacteria, such as Rhizobia species. This process involves the formation of specialized structures called nodules on the roots of the host plant. The bacteria inhabit these nodules and convert atmospheric nitrogen into ammonia, a form of nitrogen that plants can use for growth. In return, the plant provides the bacteria with carbon sources and a protected environment for growth. This mutualistic relationship helps improve soil fertility and promotes sustainable agriculture.

Phylogeny is the evolutionary history and relationship among biological entities, such as species or genes, based on their shared characteristics. In other words, it refers to the branching pattern of evolution that shows how various organisms have descended from a common ancestor over time. Phylogenetic analysis involves constructing a tree-like diagram called a phylogenetic tree, which depicts the inferred evolutionary relationships among organisms or genes based on molecular sequence data or other types of characters. This information is crucial for understanding the diversity and distribution of life on Earth, as well as for studying the emergence and spread of diseases.

'Medicago sativa' is the scientific name for a plant species more commonly known as alfalfa. In a medical context, alfalfa is often considered a herbal supplement and its medicinal properties include being a source of vitamins, minerals, and antioxidants. It has been used in traditional medicine to treat a variety of conditions such as kidney problems, asthma, arthritis, and high cholesterol levels. However, it's important to note that the effectiveness of alfalfa for these uses is not conclusively established by scientific research and its use may have potential risks or interactions with certain medications. Always consult a healthcare provider before starting any new supplement regimen.

DNA Sequence Analysis is the systematic determination of the order of nucleotides in a DNA molecule. It is a critical component of modern molecular biology, genetics, and genetic engineering. The process involves determining the exact order of the four nucleotide bases - adenine (A), guanine (G), cytosine (C), and thymine (T) - in a DNA molecule or fragment. This information is used in various applications such as identifying gene mutations, studying evolutionary relationships, developing molecular markers for breeding, and diagnosing genetic diseases.

The process of DNA Sequence Analysis typically involves several steps, including DNA extraction, PCR amplification (if necessary), purification, sequencing reaction, and electrophoresis. The resulting data is then analyzed using specialized software to determine the exact sequence of nucleotides.

In recent years, high-throughput DNA sequencing technologies have revolutionized the field of genomics, enabling the rapid and cost-effective sequencing of entire genomes. This has led to an explosion of genomic data and new insights into the genetic basis of many diseases and traits.

Rhizobium tropici is a root nodule bacterium. Martínez-Romero E, Segovia L, Mercante FM, Franco AA, Graham P, Pardo MA. (1991 ... "Rhizobium tropici". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved May 28, 2021. v t e (CS1 maint: ... "Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees". Int J Syst Bacteriol. 41 (3 ...
Rhizobium tropici strain CIAT 899 is a bacterial type strain that was isolated in 1978 from nodules on Phaseolus vulgaris in ... Rhizobium tropici Martinez-Romero et al. 49672 ™ Download Genome Learn about our Enhanced Authentication Initiative Rhizobium ... To download a certificate of analysis for Rhizobium tropici Martinez-Romero et al. (49672), enter the lot number exactly as it ... To download a certificate of origin for Rhizobium tropici Martinez-Romero et al. (49672), enter the lot number exactly as it ...
Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes-with an emphasis on common bean (Phaseolus ... Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular ... Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular ... Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, ...
The interspecific hybrids showed high nodulation potential with Rhizobium tropici 899 that was similar to Verano. ... The interspecific hybrids showed high nodulation potential with Rhizobium tropici 899 that was similar to Verano. ... and Rhizobium CIAT tropici 899 (common beans). Nodulation was evaluated 21 days after inoculation. Interspecific hybrids INB ... and Rhizobium CIAT tropici 899 (common beans). Nodulation was evaluated 21 days after inoculation. Interspecific hybrids INB ...
Rhizobium tropici chromosomal citrate synthase gene. Applied and Environmental Microbiology. , 61. (11), 3992-3997. . * ... Rhizobium tropici chromosomal citrate synthase gene. Applied and Environmental Microbiology. , 61. (11), 3992-3997. . * ... Expression pattern of Rhizobium etli ccmIEFH genes involved in c-type cytochrome maturation. Gene. , 250. (1-2), 149-157. . ... Expression pattern of Rhizobium etli ccmIEFH genes involved in c-type cytochrome maturation. Gene. , 250. (1-2), 149-157. . ...
Rhizobia phylogenetically related to common bean symbionts Rhizobium giardinii and Rhizobium tropici isolated from peanut ...
Nogales J, Campos R, BenAbdelkhalek H, Olivares J, Lluch C, Sanjuan J: Rhizobium tropici genes involved in free-living salt ... lipopolysaccharide biosynthesis which has been found necessary for adaptation to high external NaCl stress in Rhizobium tropici ...
Rhizobium tropici. s. 4. 13. Agrobacterium rhizogenes. s. 4. 11. Agrobacterium rhizogenes. s. 4. 12. ...
Rhizobium tropici CIAT 899. s. 7. 2. Lentzea albidocapillata. s. 7. 2. ...
Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which ... Genome sequence of the acid-tolerant strain Rhizobium sp. LPU83.. Wibberg D, Tejerizo GT, Del Papa MF, Martini C, Pühler A, ... H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an ... H13-3, formerly known as Rhizobium lupini H13-3, is a soil bacterium that was isolated from the rhizosphere of Lupinus luteus. ...
Rhizobium capsici sp. nov., isolated from root tumor of a green bell pepper (Capsicum annuum var. grossum) plant. Antonie Van ... Rhizobium straminoryzae sp. nov., isolated from the surface of rice straw. Int J Syst Evol Microbiol. 2014 Sep; 64(Pt 9):2962- ... Rhizobium induces DNA damage in Caenorhabditis elegans intestinal cells. Proc Natl Acad Sci U S A. 2019 02 26; 116(9):3784-3792 ... "Rhizobium" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ...
Em outro experimento, avaliou-se o número de nódulos formados após inoculação com Rhizobium tropici. Análises de variância ... In another experiment, nodule number was assessed after inoculation with Rhizobium tropici. Analysis of variance was used to ...
Variability of isolated colonies in bean nodulating Rhizobium strains before and after exposure to high temperature Facebook ... R. tropici strains (with the exception of FJ2.21) were more stable than R. leguminosarum bv. phaseoli strains because no ... Estirpes de Rhizobium eficientes na nodulação do feijoeiro, isoladas de diferentes solos da região do cultivo dessa leguminosa ... Effects of high temperature on survival, symbiotic performance and genomic modifications of bean nodulating Rhizobium strains ...
... , Rhizobium tropici , Rhizobium , Simbiose/genética , Rhizobium tropici/genética , Rhizobium tropici/metabolismo , ... CNPSo 4039T), and Rhizobium centroccidentale sp. nov. (CNPSo 4062T).. Assuntos. Phaseolus , Rhizobium , Brasil , Rhizobium/ ... CNPSo 3464T), Rhizobium atlanticum sp. nov. (CNPSo 3490T), Rhizobium aureum sp. nov. (CNPSo 3968T), Rhizobium pantanalense sp. ... CRK12: A Key Player in Regulating the Phaseolus vulgaris-Rhizobium tropici Symbiotic Interaction. ...
In this study, root nodules wereinduced by Rhizobium tropici CIAT899 in two Phaseolusvulgaris recombinant inbred lines (RIL); ...
Viable populations of rhizobia differed significantly between carriers and rhizobia strains (P,0.05). Rhizobium tropici CIAT899 ... BIOFIX is one of the commercially available rhizobia/legume inoculants in East Africa, whose standard is at least 109 rhizobia ... and Rhizobium tropici CIAT 899 for common bean (Phaseolus vulgaris) over 165 days, using the drop plate method on Congo Red ... Adoption of legume inoculation with rhizobia by small-scale farmers in East Africa, and the resultant increase in biological ...
... pyrene by Rhizobium tropici CIAT 899 in liquid culture medium. Applied Soil Ecology 63, pp. 105-111. DOI: 10.1016/j.apsoil. ...
Co-inoculation of maize with Azospirillum brasilense and Rhizobium tropici as a strategy to mitigate salinity stress. Funct. ... Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability. Biol. Fertil. ...
Effects of Rhizobium tropici azide-resistant mutants on growth, nitrogen nutrition and nodulation of common bean (Phaseolus ... Inoculation with rhizobium inoculum in the shade. Rhizobium inoculum was added at the rate of 50 ml for each kilogram of seeds ... Influence of Rhizobium, Pseudomonas and Mycorrhiza on Some Physiological Traits of Red Beans (Phaseolus vulgaris) under ... Response of yield and yield components of six genotypes of Pinto beans (Phaseolus vulgaris) inoculation with Rhizobium phaseoli ...
Herbicide action on the growth of Rhizobium tropici strains. Planta Daninha 24(3): 457-465 ...
... tropici & --- & --- & --- & --- & --- & & 11139 & E. fredii & --- & --- & --- & --- & --- & & 13798 & E. medicae ... Several agar formulations were tested for the ability to grow rhizobia. The best media for growth were YMA, YMA+Ca, and R2A. ... The primer pair nodA1 and nodA2 amplified type 3, 4 and 5 nodA genes from Mesorhizobium and Rhizobium sequences yielding a 600- ... For cycle sequencing of Mesorhizobium and Rhizobium strains, the PCR primer was used as the cycle sequencing primer. For ...
Rhizobium etli B3.585.900.337 Rhizobium leguminosarum B3.585.900.450 Rhizobium phaseoli B3.585.900.600 Rhizobium tropici B3.585 ...
Rhizobium etli B3.585.900.337 Rhizobium leguminosarum B3.585.900.450 Rhizobium phaseoli B3.585.900.600 Rhizobium tropici B3.585 ...
Rhizobium etli B3.585.900.337 Rhizobium leguminosarum B3.585.900.450 Rhizobium phaseoli B3.585.900.600 Rhizobium tropici B3.585 ...
Rhizobium etli B3.585.900.337 Rhizobium leguminosarum B3.585.900.450 Rhizobium phaseoli B3.585.900.600 Rhizobium tropici B3.585 ...
Rhizobium etli B3.585.900.337 Rhizobium leguminosarum B3.585.900.450 Rhizobium phaseoli B3.585.900.600 Rhizobium tropici B3.585 ...
Tajini F, Trabelsi M, Drevon J (2012). Combined Inoculation with Glomus Intraradices and Rhizobium tropici CIAT 899 Increases ... Zahir ZA, Yasin HM, Naveed M, Anjum MA, Khalid DM (2010). L-Tryptophan Application Enhances the Effectiveness of Rhizobium ... Özturan Akman Y (2017). Rhizobium ve Mikoriza Uygulamalarının Fasulye (Phaseolus vulgaris L.)nin Tane Verimi ve Bazı Tarımsal ... Bildirici N (2003). Van-Gevaş Koşullarında Farklı Azot ve Fosfor Dozları ile Bakteri Aşılamasının (Rhizobium phaseoli) Şeker ...
Secondary metabolites of Rhizobium tropici CIAT 899 added to Bradyrhizobium spp. inoculant promote soybean growth and increase ... New rhizobia strains isolated from the Amazon region fix atmospheric nitrogen in symbiosis with cowpea and increase its yield. ...
Crop rotation, green manuring and inoculation of Rhizobium tropici and Azospirillu.... Crop rotation, green manure and ... inoculation of Rhizobium tropici and Azospirillum .... Management of phosphorus fertilization with the use or non-inoculation ...
Inoculation methods of Rhizobium tropici and Azospirillum brasilense on common bea.... Assessment of efficiency of phosphorus ...
  • Rhizobium tropici strain CIAT 899 is a bacterial type strain that was isolated in 1978 from nodules on Phaseolus vulgaris in Colombia. (atcc.org)
  • Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes-with an emphasis on common bean (Phaseolus vulgaris)-and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. (us.es)
  • Twelve interspecific hybrids, three genotypes of tepary bean, and common beans 'Verano' and TARS-LFR1 were inoculated separately with Bradyrhizobium strain 3254 (interspecific hybrids and tepary) and Rhizobium CIAT tropici 899 (common beans). (usda.gov)
  • We examined the effect of carrier material and storage conditions on the populations of two industry standard rhizobia, Bradyrhizobium japonicum USDA 110 for soybean ( Glycine max ) and Rhizobium tropici CIAT 899 for common bean ( Phaseolus vulgaris ) over 165 days, using the drop plate method on Congo Red Yeast Extract Mannitol Agar. (n2africa.org)
  • Rhizobium leguminosarum bv. (effectors.org)
  • Mutation in the pssZ Gene Negatively Impacts Exopolysaccharide Synthesis, Surface Properties, and Symbiosis of Rhizobium leguminosarum bv. (us.es)
  • Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. (us.es)
  • The interspecific hybrids showed high nodulation potential with Rhizobium tropici 899 that was similar to Verano. (usda.gov)
  • Regulation of Resource Partitioning Coordinates Nitrogen and Rhizobia Responses and Autoregulation of Nodulation in Medicago truncatula. (harvard.edu)
  • Rhizobium tropici CIAT899, prepared with filter mud carrier, achieved a shelf-life of 135 days and B. japonicum USDA110 contained over 10 9 cells g -1 for 105 days. (n2africa.org)
  • Rhizobium induces DNA damage in Caenorhabditis elegans intestinal cells. (harvard.edu)
  • H13-3, formerly known as Rhizobium lupini H13-3, is a soil bacterium that was isolated from the rhizosphere of Lupinus luteus. (uni-bielefeld.de)
  • This strain is propagated aerobically in Rhizobium X medium. (atcc.org)
  • The phylogenetic tree of the 16S rRNA gene split the 13 strains into two large clades, seven in the R. etli and six in the R. tropici clade. (bvsalud.org)
  • Adoption of legume inoculation with rhizobia by small-scale farmers in East Africa, and the resultant increase in biological nitrogen fixation requires that quality inoculants meet minimum standards. (n2africa.org)
  • BIOFIX is one of the commercially available rhizobia/legume inoculants in East Africa, whose standard is at least 10 9 rhizobia g -1 . (n2africa.org)
  • The rhizobial type 3 secretion system: the Dr. Jekyll and Mr. Hyde in the rhizobium-legume symbiosis. (us.es)
  • 1991). "Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. (wikipedia.org)
  • Van-Gevaş Koşullarında Farklı Azot ve Fosfor Dozları ile Bakteri Aşılamasının (Rhizobium phaseoli) Şeker Fasulyesi (Phaseolus vulgaris L) Çeşidinin Verim ve Verim Öğeleri Üzerine Etkisi. (selcuk.edu.tr)
  • 2018. Co-inoculation of maize with Azospirillum brasilense and Rhizobium tropici as a strategy to mitigate salinity stress. (uptc.edu.co)
  • 2013. Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability. (uptc.edu.co)
  • This graph shows the total number of publications written about "Rhizobium" by people in Harvard Catalyst Profiles by year, and whether "Rhizobium" was a major or minor topic of these publication. (harvard.edu)
  • The symbiotic interaction between rhizobia and legumes that leads to nodule formation is a complex chemical conversation involving plant release of nod -gene inducing signal molecules and bacterial secretion of lipo-chito-oligossacharide nodulation factors. (frontiersin.org)
  • Although symbiotic rhizobia are capable of triggering biological outcomes with direct and indirect effects on plant mineral nutrition, insect pest and disease resistance, a greater understanding of the mechanisms involved remains a challenge in tapping the maximum benefits of the molecules involved. (frontiersin.org)
  • These microsymbionts are distributed among major bacterial genera of the alpha-proteobacteria such as Rhizobium, Bradyrhizobium, Azorhizobium, Mesorhizobium, Ensifer ( Sinorhizobium ), Neorhizobium, Pararhizobium , and Allorhizobium , collectively termed "rhizobia", which can form symbiotic associations with diverse legumes. (frontiersin.org)
  • Symbiotic nitrogenfixation by rhizobia with species of leguminous plants is widespread in nature.The promiscuous nature of common bean (Phaseolus vulgaris) hostingdifferent rhizobial species is known. (conicet.gov.ar)
  • The lines were inoculated with a mixture of Rhizobium etli and R. tropici to promote symbiotic nitrogen fixation (SNF). (ucr.ac.cr)
  • 1991). "Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. (wikipedia.org)
  • Complete genomesfrom some of them are available (2,4,5), and genome comparisons have led thereevaluation of the taxonomic status of the Rhizobium species thatnodulate bean (3). (conicet.gov.ar)
  • Some of these metabolites can enhance plant growth via the suppression of the deleterious effects of other antagonistic molecules, as exemplified by the reduction in the deleterious effect of ethylene by ACC deaminase synthesized by rhizobia. (frontiersin.org)
  • 9 Also Escherichia coli expressing Candida albicans Metallothionine gene and Rhizobium tropici Phytochelatin synthetase gene showed enhanced production of silver nanoparticles 10 and selenium nanoparticles 11 respectively. (microbiologyjournal.org)

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