A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
A species of gram-negative, aerobic bacteria that is found in soil and which causes formation of root nodules on some, but not all, types of field pea, lentil, kidney bean, and clover.
A species of gram-negative bacteria and nitrogen innoculant of PHASEOLUS VULGARIS.
A species of gram-negative bacteria and an nitrogen inoculum that displays a high intrinsic tolerance to acidity.
The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
A species of gram-negative, aerobic bacteria that causes formation of root nodules on some, but not all, types of sweet clover, MEDICAGO SATIVA, and fenugreek.
A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
Knobbed structures formed from and attached to plant roots, especially of LEGUMES, which result from symbiotic infection by nitrogen fixing bacteria such as RHIZOBIUM or FRANKIA. Root nodules are structures related to MYCORRHIZAE formed by symbiotic associations with fungi.
A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.
The formation of a nitrogen-fixing cell mass on PLANT ROOTS following symbiotic infection by nitrogen-fixing bacteria such as RHIZOBIUM or FRANKIA.
A genus of gram-negative, aerobic, nonsporeforming rods which usually contain granules of poly-beta-hydroxybutyrate. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
The functional hereditary units of BACTERIA.
A plant genus in the family FABACEAE which is the source of edible beans and the lectin PHYTOHEMAGGLUTININS.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
A genus of gram-negative, aerobic, rod-shaped bacteria usually containing granules of poly-beta-hydroxybutyrate. They characteristically invade the root hairs of leguminous plants and act as intracellular symbionts.
Proteins found in any species of bacterium.
A genus of gram-negative, rod-shaped bacteria in the family PHYLLOBACTERIACEAE. They are able to invade root-hairs of a wide range of plants, inciting the production of PLANT ROOT NODULES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A plant genus of the family FABACEAE. It is distinct from Sweet Clover (MELILOTUS), from Bush Clover (LESPEDEZA), and from Red Clover (TRIFOLIUM).
A variable annual leguminous vine (Pisum sativum) that is cultivated for its rounded smooth or wrinkled edible protein-rich seeds, the seed of the pea, and the immature pods with their included seeds. (From Webster's New Collegiate Dictionary, 1973)
Polysaccharides found in bacteria and in capsules thereof.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
The relationships of groups of organisms as reflected by their genetic makeup.
A plant species of the family FABACEAE used to study GENETICS because it is DIPLOID, self fertile, has a small genome, and short generation time.
A plant species of the genus VICIA, family FABACEAE. The seed is used for food and contains THIOCYANATES such as prunasin, cyanoalanine, cyanogen, and vicine.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
An enzyme system that catalyzes the fixing of nitrogen in soil bacteria and blue-green algae (CYANOBACTERIA). EC 1.18.6.1.
A hemoglobin-like oxygen-binding hemeprotein present in the nitrogen-fixing root nodules of leguminous plants. The red pigment has a molecular weight approximately 1/4 that of hemoglobin and has been suggested to act as an oxido-reduction catalyst in symbiotic nitrogen fixation.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
A plant genus of the family FABACEAE.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A plant genus of the family FABACEAE.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.
A plant genus of the family FABACEAE that contains tephrorin, tephrosone, and C-prenylflavonoids.
A plant genus of the family FABACEAE that is widely used as ground cover and forage and known for the edible beans, VICIA FABA.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A class in the phylum PROTEOBACTERIA comprised mostly of two major phenotypes: purple non-sulfur bacteria and aerobic bacteriochlorophyll-containing bacteria.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.
A species of gram-negative bacteria functioning as a nitrogen inoculum for dry beans, especially species in the genus PHASEOLUS.
A plant genus of the family FABACEAE. The gums and tanning agents obtained from Acacia are called GUM ARABIC. The common name of catechu is more often used for Areca catechu (ARECA).
A plant genus of the family FABACEAE that contains kukulkanin, a CHALCONE.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Symbiotic combination (dual organism) of the MYCELIUM of FUNGI with the roots of plants (PLANT ROOTS). The roots of almost all higher plants exhibit this mutually beneficial relationship, whereby the fungus supplies water and mineral salts to the plant, and the plant supplies CARBOHYDRATES to the fungus. There are two major types of mycorrhizae: ectomycorrhizae and endomycorrhizae.
A genus of gram-negative, aerobic, rod-shaped bacteria, in the family BURKHOLDERIACEAE, that are mobile by means of peritrichous FLAGELLA. The genus was formerly called Wautersia and species in this genus were formerly in the genus RALSTONIA.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
A family of small, gram-negative organisms, often parasitic in humans and other animals, causing diseases that may be transmitted by invertebrate vectors.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.
A plant family of the order Urticales, subclass Hamamelidae, class Magnoliopsida. It is most notable for the members, Cannabis and Hops.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Beneficial microorganisms (bacteria or fungi) encapsulated in carrier material and applied to the environment for remediation and enhancement of agricultural productivity.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Lipid A is the biologically active component of lipopolysaccharides. It shows strong endotoxic activity and exhibits immunogenic properties.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A plant genus of the family FABACEAE.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
5,7,3',4'-tetrahydroxy-flavone, one of the FLAVONES.
Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.

Thermostability reinforcement through a combination of thermostability-related mutations of N-carbamyl-D-amino acid amidohydrolase. (1/1949)

For the improvement of N-carbamyl-D-amino acid amidohydrolase (DCase), which can be used for the industrial production of D-amino acids, the stability of DCase from Agrobacterium sp. KNK712 was improved through various combinations of thermostability-related mutations. The thermostable temperature (defined as the temperature on heat treatment for 10 min that caused a decrease in the DCase activity of 50%) of the enzyme which had three amino acids, H57Y, P203E, and V236A, replaced was increased by about 19 degrees C. The mutant DCase, designated as 455M, was purified and its enzymatic properties were studied. The enzyme had highly increased stability against not only temperature but also pH, the optimal temperature of the enzyme being about 75 degrees C. The substrate specificity of the enzyme for various N-carbamyl-D-amino acids was changed little in comparison with that of the native enzyme. Enzymochemical parameters were also measured.  (+info)

Mutation in GDP-fucose synthesis genes of Sinorhizobium fredii alters Nod factors and significantly decreases competitiveness to nodulate soybeans. (2/1949)

We mutagenized Sinorhizobium fredii HH103-1 with Tn5-B20 and screened about 2,000 colonies for increased beta-galactosidase activity in the presence of the flavonoid naringenin. One mutant, designated SVQ287, produces lipochitooligosaccharide Nod factors (LCOs) that differ from those of the parental strain. The nonreducing N-acetylglucosamine residues of all of the LCOs of mutant SVQ287 lack fucose and 2-O-methylfucose substituents. In addition, SVQ287 synthesizes an LCO with an unusually long, C20:1 fatty acyl side chain. The transposon insertion of mutant SVQ287 lies within a 1.1-kb HindIII fragment. This and an adjacent 2.4-kb HindIII fragment were sequenced. The sequence contains the 3' end of noeK, nodZ, and noeL (the gene interrupted by Tn5-B20), and the 5' end of nolK, all in the same orientation. Although each of these genes has a similarly oriented counterpart on the symbiosis plasmid of the broad-host-range Rhizobium sp. strain NGR234, there are significant differences in the noeK/nodZ intergenic region. Based on amino acid sequence homology, noeL encodes GDP-D-mannose dehydratase, an enzyme involved in the synthesis of GDP-L-fucose, and nolK encodes a NAD-dependent nucleotide sugar epimerase/dehydrogenase. We show that expression of the noeL gene is under the control of NodD1 in S. fredii and is most probably mediated by the nod box that precedes nodZ. Transposon insertion into neoL has two impacts on symbiosis with Williams soybean: nodulation rate is reduced slightly and competitiveness for nodulation is decreased significantly. Mutant SVQ287 retains its ability to form nitrogen-fixing nodules on other legumes, but final nodule number is attenuated on Cajanus cajan.  (+info)

The nolL gene from Rhizobium etli determines nodulation efficiency by mediating the acetylation of the fucosyl residue in the nodulation factor. (3/1949)

The nodulation factors (Nod factors) of Rhizobium etli and R. loti carry a 4-O-acetyl-L-fucosyl group at the reducing end. It has been claimed, based on sequence analysis, that NolL from R. loti participates in the 4-O-acetylation of the fucosyl residue of the Nod factors, as an acetyl-transferase (D. B. Scott, C. A. Young, J. M. Collins-Emerson, E. A. Terzaghi, E. S. Rockman, P. A. Lewis, and C. E. Pankhurst. Mol. Plant-Microbe Interact. 9:187-197, 1996). Further support for this hypothesis was obtained by studying the production of Nod factors in an R. etli nolL::Km mutant. Chromatographic and mass spectrometry analysis of the Nod factors produced by this strain showed that they lack the acetyl-fucosyl substituent, having a fucosyl group instead. Acetyl-fucosylation was restored upon complementation with a wild-type nolL gene. These results indicate that the nolL gene determines 4-O-acetylation of the fucosyl residue in Nod factors. Analysis of the predicted NolL polypeptide suggests a transmembranal location and that it belongs to the family of integral membrane transacylases (J. M. Slauch, A. A. Lee, M. J. Mahan, and J. J. Mekalanos. J. Bacteriol. 178:5904-5909, 1996). NolL from R. loti was also proposed to function as a transporter; our results show that NolL does not determine a differential secretion of Nod factors from the cell. We also performed plant assays that indicate that acetylation of the fucose conditions efficient nodulation by R. etli of some Phaseolus vulgaris cultivars, as well as of an alternate host (Vigna umbellata).  (+info)

Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. (4/1949)

Poly(3-hydroxyalkanoates) (PHAs) are a class of microbially produced polyesters that have potential applications as conventional plastics, specifically thermoplastic elastomers. A wealth of biological diversity in PHA formation exists, with at least 100 different PHA constituents and at least five different dedicated PHA biosynthetic pathways. This diversity, in combination with classical microbial physiology and modern molecular biology, has now opened up this area for genetic and metabolic engineering to develop optimal PHA-producing organisms. Commercial processes for PHA production were initially developed by W. R. Grace in the 1960s and later developed by Imperial Chemical Industries, Ltd., in the United Kingdom in the 1970s and 1980s. Since the early 1990s, Metabolix Inc. and Monsanto have been the driving forces behind the commercial exploitation of PHA polymers in the United States. The gram-negative bacterium Ralstonia eutropha, formerly known as Alcaligenes eutrophus, has generally been used as the production organism of choice, and intracellular accumulation of PHA of over 90% of the cell dry weight have been reported. The advent of molecular biological techniques and a developing environmental awareness initiated a renewed scientific interest in PHAs, and the biosynthetic machinery for PHA metabolism has been studied in great detail over the last two decades. Because the structure and monomeric composition of PHAs determine the applications for each type of polymer, a variety of polymers have been synthesized by cofeeding of various substrates or by metabolic engineering of the production organism. Classical microbiology and modern molecular bacterial physiology have been brought together to decipher the intricacies of PHA metabolism both for production purposes and for the unraveling of the natural role of PHAs. This review provides an overview of the different PHA biosynthetic systems and their genetic background, followed by a detailed summation of how this natural diversity is being used to develop commercially attractive, recombinant processes for the large-scale production of PHAs.  (+info)

Sequence and molecular analysis of the Rhizobium etli glsA gene, encoding a thermolabile glutaminase. (5/1949)

We sequenced a 2.1 kb fragment of DNA carrying the structural glsA gene, which codes for the Rhizobium etli thermolabile glutaminase (A). The glsA gene complements the R. etli LM16 mutant that lacks glutaminase A activity, and is expressed in the heterologous host Sinorhizobium meliloti. The deduced amino acid sequence consists of 309 residues, with a calculated molecular mass of 33 kDa. The amino acid sequence shares 53% and 43% identity with two hypothetical glutaminases of E. coli; 42% identity with liver-type; 38% identity with kidney-type glutaminase; 41% and 40% identity hypothetical glutaminases of Bacillus subtilis; and 41% and 37% identity with two putative glutaminases of Caenorhabditis elegans. The glsA gene represents the first glutaminase gene cloned and sequenced in prokaryotes.  (+info)

Isolation and characterization of the catalase gene from Rhizobium sp. SNU003, a root nodule symbiont of Canavalia lineata. (6/1949)

A catalase gene from Rhizobium sp. SNU003, a root nodule symbiont of Canavalia lineata, was cloned and its nucleotide sequence was determined. The Rhizobium DNA of about 280 bp was amplified using two PCR primers synthesized from the conserved sequences of the type I catalase gene. The nucleotide sequence of the amplified fragment revealed three regions that were conserved in the catalase, showing it as being part of the catalase gene. A genomic Southern hybridization using this fragment as a probe showed that the 5.5 kb PstI, 1.8 kb EcoRI, and 0.7 kb StyI fragments hybridized strongly with the probe. The Rhizobium genomic library constructed into the EMBL3 vector was screened, and one catalase clone was selected. The nucleotide sequence of the 5.5 kb PstI fragment from the clone revealed an open reading frame of 1455 bp, encoding a polypeptide of 485 amino acids with a molecular mass of 54,958 Da and a pI of 6.54. The predicted amino acid sequence of the catalase is 66.3% identical to that of Bacteroides fragilis, but was only 53.3% identical to the Rhizobium meliloti catalase.  (+info)

Rhizobium sp. strain NGR234 and R. fredii USDA257 share exceptionally broad, nested host ranges. (7/1949)

Genetically, Rhizobium sp. strain NGR234 and R. fredii USDA257 are closely related. Small differences in their nodulation genes result in NGR234 secreting larger amounts of more diverse lipo-oligosaccharidic Nod factors than USDA257. What effects these differences have on nodulation were analyzed by inoculating 452 species of legumes, representing all three subfamilies of the Leguminosae, as well as the nonlegume Parasponia andersonii, with both strains. The two bacteria nodulated P. andersonii, induced ineffective outgrowths on Delonix regia, and nodulated Chamaecrista fasciculata, a member of the only nodulating genus of the Caesalpinieae tested. Both strains nodulated a range of mimosoid legumes, especially the Australian species of Acacia, and the tribe Ingeae. Highest compatibilities were found with the papilionoid tribes Phaseoleae and Desmodieae. On Vigna spp. (Phaseoleae), both bacteria formed more effective symbioses than rhizobia of the "cowpea" (V. unguiculata) miscellany. USDA257 nodulated an exact subset (79 genera) of the NGR234 hosts (112 genera). If only one of the bacteria formed effective, nitrogen-fixing nodules it was usually NGR234. The only exceptions were with Apios americana, Glycine max, and G. soja. Few correlations can be drawn between Nod-factor substituents and the ability to nodulate specific legumes. Relationships between the ability to nodulate and the origin of the host were not apparent. As both P. andersonii and NGR234 originate from Indonesia/Malaysia/Papua New Guinea, and NGR234's preferred hosts (Desmodiinae/Phaseoleae) are largely Asian, we suggest that broad host range originated in Southeast Asia and spread outward.  (+info)

Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells. (8/1949)

TraR is an Agrobacterium transcriptional regulator whose activity requires the pheromone N-3-oxooctanoyl-L-homoserine lactone. TraR was purified as a complex with the pheromone and contained one pheromone molecule per protein monomer. TraR-pheromone complexes bound to a single DNA site and activated two promoters that flank this site. Promoter expression was elevated 30-fold by using a supercoiled template. Pheromone binding increased the affinity of TraR for this binding site. Pheromone also increased TraR abundance in vivo by causing a 20-fold decrease in TraR turnover rates.  (+info)

Rhizobium, Agrobacterium and Allorhizobium are genera within the bacterial family Rhizobiaceae, together with Sinorhizobium. The species of Agrobacterium, Agrobacterium tumefaciens (syn. Agrobacterium radiobacter), Agrobacterium rhizogenes, Agrobacterium rubi and Agrobacterium vitis, together with Allorhizobium undicola, form a monophyletic group with all Rhizobium species, based on comparative 16S rDNA analyses. Agrobacterium is an artificial genus comprising plant-pathogenic species. The monophyletic nature of Agrobacterium, Allorhizobium and Rhizobium and their common phenotypic generic circumscription support their amalgamation into a single genus, Rhizobium. Agrobacterium tumefaciens was conserved as the type species of Agrobacterium, but the epithet radiobacter would take precedence as Rhizobium radiobacter in the revised genus. The proposed new combinations are Rhizobium radiobacter, Rhizobium rhizogenes, Rhizobium rubi, Rhizobium undicola and Rhizobium vitis.
Rhizobium fredii is a fast-growing rhizobium isolated from the primitive Chinese soybean cultivar Peking and from the wild soybean Glycine soja. This rhizobium harbors nif genes on 150- to 200-megadalton plasmids. By passage on acridine orange plates, we obtained a mutant of R. fredii USDA 206 cured of the 197-megadalton plasmid (USDA 206C) which carries both nif and nod genes. This strain, however, has retained its symbiotic effectiveness. Probing EcoRI digests of wild-type and cured plasmid DNA with a 2.2-kilobase nif DH fragment from Rhizobium meliloti has shown four homologous fragments in the wild-type strain (4.2, 4.9, 10, and 11 kilobases) and two fragments in the cured strain (4.2 and 10 kilobases). EcoRI digests of total DNA show four major bands of homology (4.2, 4.9, 5.8, and 13 kilobases) in both the wild-type and cured strains. The presence of major bands of homology in the total DNA not present in the plasmid DNA indicated chromosomal nif genes. Probing of HindIII digests of total and
Nodulation (nod) gene expression in Rhizobium meliloti requires plant inducers and the activating protein product of the nodD gene. We have examined three genes in R. meliloti which have nodD activity and sequence homology. These three nodD genes are designated nodD1, nodD2 and nodD3, and have distinctive properties. The nodD1 gene product activates expression of the nodABC operon, as measured by a nodC-lacZ fusion or by transcript analysis, in the presence of crude seed or plant wash or the inducer, luteolin. The nodD3 gene product can cause a high basal (uninduced) level of nodC-lacZ expression and nodABC transcripts which is relatively unaffected by inducers. The effect of nodD3 is dependent on the presence of another gene, syrM (symbiotic regulator). By primer extension analysis we determined that the transcription start site is the same for nodD1 plus luteolin or nodD3-syrM mediated expression of nodA and nodH mRNAs. syrM also enhances the expression of another symbiotically important ...
Other names: A. rhizogenes, ATCC 11325, Agrobacterium biovar 2, Agrobacterium genomic group 10, Agrobacterium genomic species 10, Agrobacterium genomosp. 10, Agrobacterium rhizogenes, Agrobacterium rhizogenes (RI plasmid PRI1724), Agrobacterium rhizogenes (RI plasmid PRI8196), Agrobacterium rhizogenes (RI plasmid PRIA4B), CFBP 5520, CIP 104328, DSM 30148, ICMP 5794, IFO 13257, JCM 20919, LMG 150, NBRC 13257, NCPPB 2991, Rhizobium rhizogenes, Rhizobium sp. LMG 9509 ...
Other names: A. rhizogenes, ATCC 11325, Agrobacterium biovar 2, Agrobacterium genomic group 10, Agrobacterium genomic species 10, Agrobacterium genomosp. 10, Agrobacterium rhizogenes, Agrobacterium rhizogenes (RI plasmid PRI1724), Agrobacterium rhizogenes (RI plasmid PRI8196), Agrobacterium rhizogenes (RI plasmid PRIA4B), CFBP 5520, CIP 104328, DSM 30148, ICMP 5794, IFO 13257, JCM 20919, LMG 150, NBRC 13257, NCPPB 2991, Rhizobium rhizogenes, Rhizobium sp. LMG 9509 ...
Pesticide properties for Agrobacterium radiobacter strain K1026, including approvals, environmental fate, eco-toxicity and human health issues
The Rhizobium common nod gene products NodABC are involved in the synthesis of the core lipochitooligosaccharide (Nod factor) structure, whereas the products of the host-specific nod genes are necessary for diverse structural modifications, which vary in different Rhizobium species. The sulfate group attached to the Rhizobium meliloti Nod signal is necessary for activity on the host plant alfalfa, while its absence renders the Nod factor active on the non-host plant vetch. This substituent is therefore a major determinant of host specificity. The exact biosynthetic pathway of Nod factors has not been fully elucidated. In particular, it is not known why some chemical modifications are introduced with high fidelity whereas others are inaccurate, giving rise to a family of different Nod factor structures produced by a single Rhizobium strain. Using protein extracts and partially purified recombinant NodH protein obtained from Escherichia coli expressing the R. meliloti nodH gene, we demonstrate ...
The plant supplies the rhizobia with energy in the form of amino acids and the . Legumes can become infected with nitrogen-fixing bacteria known as rhizobia. These bacteria live in the soil, and when a legume grows nearby a molecular communication ensues that enables the legume roots to become infected. In a process guided by both the bacteria and the plant, the rhizobia invade . Wally Eberhart, Corbis, NTB scanpix.. Update in progress: This page lists all of the current validly-published binomial names for the rhizobia , which currently consists of species in genera. Most of these bacterial species are in the Rhizobiacae family in the . The biological reduction of atmospheric Nto ammonium (nitrogen fixation) provides about of the biospheres available nitrogen. Most of this ammonium is contributed by legume- rhizobia symbioses, which are initiated by the infection of legume hosts by bacteria ( rhizobia ), resulting in formation of root nodules. Rhizobia are nitrogen-fixing bacteria that form ...
The role of soil microorganisms in plant growth, nutrient utilization, drought tolerance as well as biocontrol activity cannot be over-emphasized, especially in this era when food crisis is a global challenge. This research was therefore designed to gain genomic insights into plant growth promoting (PGP) Rhizobium species capable of enhancing soybean (Glycine max L.) seeds germination under drought condition. Rhizobium sp. strain R1, Rhizobium tropici strain R2, Rhizobium cellulosilyticum strain R3, Rhizobium taibaishanense strain R4 and Ensifer meliloti strain R5 were found to possess the entire PGP traits tested. Specifically, these rhizobial strains were able to solubilize phosphate, produce exopolysaccharide (EPS), 1-aminocyclopropane-1-carboxylate (ACC), siderophore and indole-acetic-acid (IAA). These strains also survived and grew at a temperature of 45 °C and in an acidic condition with a pH 4. Consequently, all the Rhizobium strains enhanced the germination of soybean seeds (PAN 1532 R) under
Monoclonal antibodies were used as cytochemical markers to study surface interactions between endosymbiotic Rhizobium bacteroids from pea root nodules and the encircling peribacteroid membranes, which are of plant origin. Monoclonal antibodies that react with Rhizobium lipopolysaccharide (LPS) or with a plant membrane glycoprotein were used as markers for material from the bacteroid outer membrane or the peribacteroid membrane, respectively. Membrane-enclosed bacteroids were isolated from nodule homogenates by sucrose gradient centrifugation, and the encircling peribacteroid membrane was released by mild osmotic shock treatment. Using an immunochemical technique (sandwich ELISA), it was shown that 1-5% of the LPS antigen released into the peribacteroid fraction by mild osmotic shock treatment was physically associated with peribacteroid membrane through a detergent-sensitive linkage. This association could be visualized when freshly prepared peribacteroid material was immobilized on gold grids ...
Rhizobium is a genus of Gram-negative soil bacteria that fix nitrogen. Rhizobium species form an endosymbiotic nitrogen-fixing association with roots of legumes and Parasponia. The bacteria colonize plant cells within root nodules, where they convert atmospheric nitrogen into ammonia and then provide organic nitrogenous compounds such as glutamine or ureides to the plant. The plant, in turn, provides the bacteria with organic compounds made by photosynthesis. This mutually beneficial relationship is true of all of the rhizobia, of which the Rhizobium genus is a typical example. Beijerinck in the Netherlands was the first to isolate and cultivate a microorganism from the nodules of legumes in 1888. He named it Bacillus radicicola, which is now placed in Bergeys Manual of Determinative Bacteriology under the genus Rhizobium. Rhizobium forms a symbiotic relationship with certain plants such as legumes, fixing nitrogen from the air into ammonia, which acts as a natural fertilizer for the plants. ...
Agrobacterium infection, which is widely used to generate transgenic plants, is often accompanied by T-DNA-linked mutations and transpositions in flowering plants. It is not known if Agrobacterium infection also affects the rates of point mutations, somatic homologous recombinations (SHR) and frame-shift mutations (FSM). We examined the effects of Agrobacterium infection on five types of somatic mutations using a set of mutation detector lines of Arabidopsis thaliana. To verify the effect of secreted factors, we exposed the plants to different Agrobacterium strains, including wild type (Ach5), its derivatives lacking vir genes, oncogenes or T-DNA, and the heat-killed form for 48 h post-infection; also, for a smaller set of strains, we examined the rates of three types of mutations at multiple time-points. The mutation detector lines carried a non-functional β-glucuronidase gene (GUS) and a reversion of mutated GUS to its functional form resulted in blue spots. Based on the
A motile, Gram-stain-negative, non-pigmented bacterial strain, designated MGL06T, was isolated from seawater of the South China Sea on selection medium containing 0.1 % (w/v) malachite green. Strain MGL06T showed highest 16S rRNA gene sequence similarity to Rhizobium vignae CCBAU 05176T (97.2 %), and shared 93.2-96.9 % with the type strains of other recognized Rhizobium species. Phylogenetic analyses based on 16S rRNA and housekeeping gene sequences showed that strain MGL06T belonged to the genus Rhizobium. Mean levels of DNA-DNA relatedness between strain MGL06T and R. vignae CCBAU 05176T, Rhizobium huautlense S02T and Rhizobium alkalisoli CCBAU 01393T were 20 ± 3, 18 ± 2 and 14 ± 3 %, respectively, indicating that strain MGL06T was distinct from them genetically. Strain MGL06T did not form nodules on three different legumes, and the nodD and nifH genes were also not detected by PCR or based on the draft genome sequence. Strain MGL06T contained Q-10 as the predominant ubiquinone. The major fatty
Rhizobia are composed of specific groups of bacteria that have the ability to induce symbiotic nitrogen-fixing nodules on the roots or stems of leguminous plants. Rhizobia have attracted a great attention for more than 4 decades because of their enormous agricultural and economic value in sustainable agriculture. Up to the present time, many legumes have been found to be nodulated by several rhizobial species in diverse taxonomic groups. An assessment of rhizobial diversity provides pivotal information in understanding the horizontal gene transfer among bacterial genera and species, the bacterial evolution and the symbiotic effectiveness. The classification of rhizobia is becoming increasingly complex and is revised periodically because of new findings that propose new genera and new species. Phenotypic and Genotypic Diversity of Rhizobia presents the application of conventional and molecular analyses, including numerical analysis, enzyme patterns, serological studies, plasmid profile, ...
Rhizobium spp. are found in soil. They are both free-living and found symbiotically associated with the nodules of leguminous plants. Traditionally, studies have focused on the association of these organisms with plants in nitrogen-fixing nodules, since this is regarded as the most important role of these bacteria in the environment. Rhizobium sp. are known to possess several replicons. Some, like the Rhizobium etli symbiotic plasmid p42d and the plasmid pNGR234b of Rhizobium NGR234, have been sequenced and characterized. The plasmids from these organisms are the focus of this short review.. ...
FINAL DIAGNOSIS: AGROBACTERIUM RADIOBACTER. CONTRIBUTORS NOTE:. The genus Agrobacterium are aerobic, gram negative, peritrichous bacilli which are mainly plant pathogens found in soil all over the world. Agrobacterium tumefaciens and Agrobacterium radiobacter are two species that have been isolated from human specimens although only A. radiobacter is associated with clinical symptoms (1).. Despite A. radiobacters association with significant clinical symptoms, it is considered to be of low virulence and there have been no reports of mortality from the organism alone. The only difference between the two species is the presence of a tumor-producing plasmid, the Ti plasmid, in A. tumefaciens which results in a tumorous growth in plants (2).. Agrobacterium radiobacter is infrequently recognized in clinical specimens however it has been associated with immunocompromised patients that have implanted medical devices or transcutaneous catheters (1,3,4). It was first isolated in 1967 but was not ...
The most important step of this study was to isolate Rhizobium species and determination of their potency for growth factor production. We isolated 260 type bacteria on PCA (Plate Count Agar) media from adjacent soil samples of ten leguminous plants, two non-leguminous plants and control soil (without plant). Then pure cultures of 53 nitrogen fixing bacterial strains were isolated on selective Yeast Extract Mannitol Agar (YEMA) medium. Five isolates (Ma-G1, Ch-H2, Lo-F1, Sh-J1and Ra-I2) were selected as Rhizobium hainanense and better result was regarded in their assessment for production potency of various growth factors. All Rhizobium spp. were able to fix nitrogen in media. Among these isolates, indole acetic acid (IAA) was produced by Ma-G1 and Ra-I2. The exopolysaccharide production rate of Ma-G1 was enhanced expectedly (1.25-fold increase) by treating with IAA. Unfortunately, these five strains were unable to separate soluble phosphorus content from insoluble tri-calcium phosphate (TCP). ...
Procedures based on DNA hybridization and PCR were developed for quality control of Rhizobium inoculants. Inoculants for pea and goats rue were produced by Elomestari Ltd., Juva, Finland, in sterile dry fine peat by the standard procedure used by the company. The inoculants contained Rhizobium galegae HAMBI 1174 and HAMBI 1207 and an R. leguminosarum biovar vicia strain, 16HSA, either solely or in combinations of two or three strains. DNA was isolated from 1-g samples of each peat inoculant and analyzed by nonradioactive DNA-DNA hybridization and by PCR. The hybridization probes were total DNAs from pure cultures of R. galegae HAMBI 1207 and R. leguminosarum biovar viciae 16HSA and a 264-bp strain-specific fragment from the genome of R. galegae HAMBI 1174. The total DNA probes distinguished inoculants containing R. galegae or R. leguminosarum, and the strain-specific probe distinguished inoculants containing R. galegae HAMBI 1174. The hybridization results for R. galegae were verified in a PCR ...
SALEM, S.H. (1971) Néhány inszekticid hatása a Rhizobium trifolii effektív és ineffektív törzseinek fiziológiai aktivitására. Agrokémia és talajtan, 20 (3). pp. 368-376. ...
Agrobacterium species that are pathogenic on plants, including Agrobacterium tumefaciens, A. vitis, A. rubi, and A. rhizogenes, all carry megaplasmids. By contrast, nonpathogenic strains either lack these plasmids entirely or carry mutant forms of plasmids. A strict requirement of the Ti plasmid for virulence was established through mutational analyses and by a demonstration that the introduction of Ti plasmids into Rhizobium or Phyllobacterium spp. converts these nonpathogenic species into tumor-inducing pathogens ( 2 , 3 ). Ti plasmids induce a disease called crown gall, which is typified by the formation of undifferentiated plant tumors at the plant crown (the subterranean-to-aerial transition zone). The related root-inducing or Ri megaplasmids carried by A. rhizogenes instead induce hairy root disease, which is typified by the formation of entangled masses of roots at the infection site ( 4 ).
Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a Gram-negative soil bacterium that produces hairy root disease in dicotyledonous plants. R. rhizogenes induces the formation of proliferative multiple-branched adventitious roots at the site of infection, so-called hairy roots. In the rhizosphere, plants may suffer from wounds by soil pathogens or other sources. This leads to the secretion of phenolic compounds like acetosyringone which have chemotactic effects that attract the bacteria. Under such conditions, certain bacterial genes are turned on leading to the transfer of its tDNA from its root-inducing plasmid (Ri plasmid) into the plant through the wound. After integration and expression, in vitro or under natural conditions, the hairy root phenotype is observed, which typically includes overdevelopment of a root system that is not completely geotropic, and altered (wrinkled) leaf morphology, if leaves are present. Bacterial genes may be retained within the plant. The hairy roots ...
The genetic diversity and symbiotic efficiency among indigenous rhizobia isolates obtained from native field with or without organic fertilization and superficial mineral fertilization were investigated. Eighty-six indigenous rhizobia were isolated from these fields using four common bean varieties as trap-host. The common bean varieties Mexico 309 and Rio Tibagi selected the most efficient rhizobia strains because they showed the best yields and N contents results. The genetic characterization of 36 rhizobia isolates was evaluated by using electrophoretic profiles of amplification products using primers ERIC1-R and ERIC-2. Our results demonstrated that besides the large diversity in the indigenous rhizobial community, the genotype of the trap-host probably influences the selection of the most efficient strains.
Three bacterial isolates, designated W44T, W15 and W11, were isolated from the root of Oryza officinalis grown in Wuzhou, China. These isolates were Gram-negative, aerobic, motile and rod-shaped; demonstrated cellulase and urea activities; and formed cream-coloured colonies. The 16S rRNA gene sequence analysis indicated that the similarities between strain W44T and strains W15 and W11 were 100 %; all of them belonged to the genus Rhizobium and had the highest sequence similarity to Rhizobium rosettiformans W3T (98.7 %), followed by Rhizobium ipomoeae shin9-1T (98.2 %). Sequencing of housekeeping genes (recA, atpD, rpoB and glnA) of the novel isolates revealed similarities to members of established Rhizobium species to be less than 94.3 %. The values of DNA-DNA hybridization between strain W44T and the reference strains ( R. rosettiformans W3T and R. ipomoeae shin9-1T) were 41.3 and 29.2 %, respectively. The major cellular fatty acid of strain W44T was summed feature 8 (C18 : 1ω9t and/or C18 : 1ω9c
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Rhizobium forms a symbiotic relationship with certain plants such as legumes, fixing nitrogen from the air into ammonia, which acts as a natural fertilizer for the plants. Current research is being conducted by Agricultural Research Service microbiologists to discover a way to use Rhizobiums biological nitrogen fixation. This research involves the genetic mapping of various rhizobial species with their respective symbiotic plant species, like alfalfa or soybean. The goal of this research is to increase the plants productivity without using fertilizers.[3]. In molecular biology, Rhizobium has also been identified as a contaminant of DNA extraction kit reagents and ultrapure water systems, which may lead to its erroneous appearance in microbiota or metagenomic datasets.[4] The presence of nitrogen fixing bacteria as contaminants may be due to the use of nitrogen gas in ultra-pure water production to inhibit microbial growth in storage tanks.[5]. ...
To study the effect of different temperature and carbohydrate sources, a laboratory experiment was taken and parameters are taken separately. To study the effect of different temperature on the growth of Rhizobium, YEMA medium was used for growth of Rhizobium, there is less growth as compared to 30 c, where significantly increase in biomass growth, up to 30 c temperature. To study the effect of different carbohydrate sources, the Richard medium was used to study the growth of Rhizobium. From data it was clear that, mannitol as a source there was significantly more growth of Rhizobium compared to other sources.. ...
Rhizobium spp. show chemotaxis to plant root exudates. A glycoprotein has been isolated from the root exudates of birdsfoot trefoil, Lotus corniculatus, which, at micromolar concentrations, attracts six strains of rhizobia. This glycoprotein has been given the trivial name trefoil chemotactin and contains approximately twice as much protein as carbohydrate. Gel filtration of trefoil chemotactin on a Bio-Gel A-1.5m column gave a molecular weight of approximately 60,000. Trefoil chemotactin represents a new class of chemoattractants for bacteria. ...
A 1.9 kb DNA region of Rhizobium leguminosarum biovar viciae strain VF39 capable of promoting microaerobic and symbiotic induction of the Rhizobium meliloti fixN gene was identified by heterologous complementation. Sequence analysis of this DNA region revealed the presence of two complete open reading frames, orf240 and orf114. The deduced amino acid sequence of orf240 showed significant homology to Escherichia coli Fnr and R. meliloti FixK. The major difference between ORF240 and FixK is the presence of 21 N-terminal amino acids in ORF240 that have no counterpart in FixK. A similar protein domain is also present in E. coli Fnr and is essential for the oxygen-regulated activity of this protein. Analysis of the nucleotide sequence upstream of orf240 revealed a motif similar to the NtrA-dependent promoter consensus sequence, as well as two DNA regions resembling the Fnr consensus binding sequence. A Tn5-generated mutant in orf240 lost the ability to induce the R. meliloti fixN-lacZ fusion. ...
The Rhizobium leguminosarum biovar viciae nodulation protein NodO is partially homologous to haemolysin of Escherichia coli and, like haemolysin, is secreted into the growth medium. The NodO protein can be secreted by a strain of E. coli carrying the cloned nodO gene plus the haemolysin secretion genes hlyBD, in a process that also requires the outer membrane protein encoded by tolC. The related protease secretion genes, prtDEF, from Erwinia chrysanthemi also enable E. coli to secrete NodO. The Rhizobium genes encoding the proteins required for NodO secretion are unlinked to nodO and are unlike other nod genes, since they do not require flavonoids or NodO for their expression. Although proteins similar to NodO were not found in rhizobia other than R. leguminosarum bv. viciae, several rhizobia and an Agrobacterium strain containing the cloned nodO gene were found to have the ability to secrete NodO. These observations indicate that a wide range of the Rhizobiaceae have a protein secretion ...
Alfalfa (Medicago sativa L.) suspension cultures respond to yeast elicitors with a strong alkalinization of the culture medium, a transient synthesis of activated oxygen species, and typical late defence reactions such as phytoalexin accumulation and increased peroxidase activity. The alkalinization reaction as well as the oxidative burst were also observed when tobacco (Nicotiana tabacum L.) cell-suspension cultures were treated with yeast elicitors. Depending on the degree of polymerization, N-acetyl chitin oligomers induced the alkalinization response in both plant cell-suspension cultures, while only tobacco cell cultures developed an oxidative burst. Suspension-cultured tobacco cells responded to Sinorhizobium meliloti nodulation factors with a maximal alkalinization of 0.25 pH units and a remarkable oxidative burst. In contrast, addition of Sinorhizobium meliloti nodulation factors to suspension-cultured alfalfa cells induced a slight acidificatiton of the culture medium, instead of an ...
The secreted nodulation-signaling protein NodO was purified from the supernatant of cultures of Rhizobium leguminosarum biovar viciae. The native protein has a M(r) of approximately 67,000, suggesting that it exists as a dimer since the DNA sequence predicts a M(r) of 30,002. Pure NodO protein had no protease, pectinase, or cellulase activity, and no binding was observed to lipooligosaccharide nodulation factors. Although NodO is relatively hydrophilic, it appeared to insert into liposomes and was protected by liposomes from proteolytic cleavage. When added to planar lipid bilayers, NodO formed cation-selective channels that allowed the movement of monovalent cations (K+ and Na+) across the membrane. NodO is a Ca(2+)-binding protein; in the presence of high concentrations of Ca2+, channel activity was reduced. We hypothesize that NodO plays a role in nodulation signaling by stimulating uptake of nodulation factors or by forming cation-specific channels that function synergistically with the ...
Looking for online definition of rhizobium in the Medical Dictionary? rhizobium explanation free. What is rhizobium? Meaning of rhizobium medical term. What does rhizobium mean?
Symbiotic nitrogen fixation (SNF) can provide an agronomic and economically sustainable alternative to declining soil fertility and high cost of chemical fertilizers faced by smallholder farmers in Kenya. The aim of the study was to identify highly effective indigenous rhizobia for production of commercial bean inoculants and to investigate the influence of bio-char as a soil amendment on the effectiveness of both indigenous Kenyan rhizobia and commercial inoculant in symbiosis with common bean in low fertile soils of Western Kenya. Bioprospecting was conducted in Kenya to collect rhizobia isolates capable of nodulating and fixing N in symbiotic association with common bean. Three hundred and eighty rhizobia isolates were recovered from nodules of wild and cultivated legume hosts growing along a transect of different agro-ecological zones covering about 1045 km transect. These isolates were authenticated and tested for effectiveness on climbing bean (Phaseolus vulgaris L.) var. Kenya Tamu in ...
The bacterial chromosome may be used to stably maintain foreign DNA in the mega-base range. Integration into the chromosome circumvents issues such as plasmid replication, stability, incompatibility, and copy number variance. The site-specific integrase IntA from Rhizobium etli CFN42 catalyzes a direct recombination between two specific DNA sites: attA and attD (23 bp). This recombination is stable. The aim of this work was to develop a R. etli derivative that may be used as recipient for the integration of foreign DNA in the chromosome, adapting the IntA catalyzed site-specific recombination system. To fulfill our aim, we designed a Rhizobium etli CFN42 derivative, containing a
293990028 - EP 1002868 A1 20000524 - Method to control gene expression in bacteria namely rhyzobiaceae to improve root nodule development nitrogen fixation and plant biomass production - A promintron sequence derived from an intervening sequence of the rolA gene of Agrobacterium rhizogenes strain A4 is described. The sequence is able to drive gene expression within bacteroids in all stages of nodule development in order to obtain, over the developmental time of the nodule, a constitutive expression of the gene(s) of interest. Uses of said sequence, derived vectors and recombinant bacteria are also described. IMAGE [origin: EP1002868A1] A promintron sequence derived from an intervening sequence of the rolA gene of Agrobacterium rhizogenes strain A4 is described. The sequence is able to drive gene expression within bacteroids in all stages of nodule development in order to obtain, over the developmental time of the nodule, a constitutive expression of the gene(s) of interest. Uses of said sequence,
TY - JOUR. T1 - Identification of dominant indigenous Rhizobium meliloti by plasmid profiles and intrinsic antibiotic resistance. AU - Shishido, M.. AU - Pepper, I. L.. PY - 1990. Y1 - 1990. N2 - Yields of alfalfa (Medicago sativa L.) in irrigated production agriculture in the southwest U.S.A. varies widely, but is generally higher than in other areas of the U.S.A. Since nitrogen fertilizer is rarely applied, high yields are likely to be due in part to biological N2 fixation in the symbiotic association between alfalfa and Rhizobium meliloti. Dominant types of R. meliloti responsible for N2 fixation were identified from nodule isolates collected from five locations throughout the state of Arizona, which were not known to have been inoculated. The locations were sampled in the winter of 1987 and the summer of 1988. The dominant types (≥20% nodule occupancy at each sampling site) were identified through plasmid profile analysis and intrinsic antibiotic resistance patterns. Four types were found ...
Two mutant derivatives of Rhizobium leguminosarum ANU843 defective in lipopolysaccharide (LPS) were isolated. The LPS of both mutants lacked O antigen and some sugar residues of the LPS core oligosaccharides. Genetic regions previously cloned from another Rhizobium leguminosarum wild-type isolate, strain CFN42, were used to complement these mutants. One mutant was complemented to give LPS that was apparently identical to the LPS of strain ANU843 in antigenicity, electrophoretic mobility, and sugar composition. The other mutant was complemented by a second CFN42 lps genetic region. In this case the resulting LPS contained O-antigen sugars characteristic of donor strain CFN42 and reacted weakly with antiserum against CFN42 cells, but did not react detectably with antiserum against ANU843 cells. Therefore, one of the CFN42 lps genetic regions specifies a function that is conserved between the two R. leguminosarum wild-type isolates, whereas the other region, at least in part, specifies a strain-specific
Rhizobium etli noIL protein: gene from Rhizobium etli determines nodulation efficiency by mediating the acetylation of the fucosyl residue in the nodulation factor of plants; amino acid sequence in first source
See on Scoop.it - Virology and Bioinformatics from Virology.caThe flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. This is an interesting paper, because it describes a…
Rhizobia comprise a group of α- and β-proteobacteria known for their ability to establish symbioses with several leguminous species. The rhizobium-legume interaction, characterized by an exchange of signal molecules from both partners, culminates in the formation of specific structures, called nodules, where biological nitrogen fixation takes place [1-5]. This molecular dialogue begins with the exudation of flavonoids by the host legume roots that are recognized by a LysR-type transcriptional regulator in the bacterium, the NodD protein, which triggers the expression of the so-called nodulation (nod) genes by binding to specific sequences, nod boxes (NB), located upstream of these genes. Their cognate enzymes are implied in the production of lipochitooligosaccharides, also known as Nod factors (NF), which in turns induce the formation of root nodule primordia and play an essential role in the infection process. A part from flavonoids, other rhizobial nod gene inducers have been identified, ...
We have physically and genetically characterized 20 symbiotic and 20 auxotrophic mutants of Rhizobium meliloti, the nitrogen-fixing symbiont of alfalfa (Medicago sativa), isolated by transposon Tn5 mutagenesis. A suicide plasmid mutagenesis procedure was used to generate TN-5-induced mutants, and both auxotrophic and symbiotic mutants were found at a frequency of 0.3% among strains containing random TN5 insertions. Two classes of symbiotic mutants were isolated: 4 of the 20 formed no nodules at all (Nod-), and 16 formed nodules which failed to fix nitrogen (Fix-). We used a combination of physical and genetic criteria to determine that in most cases the auxotrophic and symbiotic phenotypes could be correlated with the insertion of a single Tn5 elements. Once the Tn5 element was inserted into the R. meliloti genome, the frequency of its transposition to a new site was approximately 10-8 and the frequency of precise excision was less than 10-9. In approximately 25% of the mutant strains, phage ...
A gene, cpaA, with similarity to calcium proton antiporters has been identified adjacent to lpcAB in Rhizobium leguminosarum. LpcA is a galactosyl transferase while LpcB is a 2-keto-3-deoxyoctonate transferase, both of which are required to form the lipopolysaccharide (LPS) core in R. leguminosarum. Mutations in lpcAB result in a rough LPS phenotype with a requirement for elevated calcium concentrations to allow growth, suggesting that truncation of the LPS core exposes a highly negatively charged molecule. This is consistent with the LPS core being one of the main sites for binding calcium in the Gram-negative outer membrane. Strain RU1109 (cpaA::Tn5-lacZ) has a normal LPS layer, as measured by silver staining and Western blotting. This indicates that cpaA mutants are not grossly affected in their LPS layer. LacZ fusion analysis indicates that cpaA is constitutively expressed and is not directly regulated by the calcium concentration. Over-expression of cpaA increased the concentration of calcium
44,203-229. , Hirsch, P. , Hooykaas, P J J , and Schilperoort, R. A (1983) A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Tr-plasmrd. Nature 303, 179,180. 8. Stachel, S. E and Nester, E W. (1986) The genetic and transcriptional organization of the vir region of the A6 TI plasmid of Agrobacterium tumefaciens. EMBO J. $1445-1454 9. Stachel, S E and Zambryski, P (1986) virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens. Biol. Rep. 10, 12-36. &APTER 7 Leaf Disk Transformation Ian S. Curtis, Michael R. Davey, and J. Brian Power 1. Introduction Reliable and efficient methods of transferring cloned genes into plants are essential for engineering crops with desired traits. The Gram-negative soil bacteria, Agrobacterium tumefaciens and A. rhizogenes, are natural genetic engineers, capable of transforming a range of dicotyledonous plants by transferring plasmid-encoded genes into recipient ...
Homologues of the plasmid replicator gene repC were detected and characterized in a sample of Rhizobium leguminosarum strains. Conserved PCR primers were designed from published sequences of repC; they amplified a fragment of about 750 bp from 39 out of 41 strains tested, and also from several Sinorhizobium strains, including S. meliloti. Restriction endonuclease digestion showed that the PCR product from individual strains, though uniform in size, was often heterogeneous in sequence. PCR products from 24 field isolates of R. leguminosarum from France, Germany and the UK were cloned and partially sequenced from both ends. Phylogenies constructed from the 5′ and 3′ ends (200 bp each) were largely congruent and demonstrated four clearly defined groups plus several unique strains. Published Agrobacterium repC sequences fall within the phylogeny of R. leguminosarum sequences, though not within any of the four groups. Specific pairs of PCR primers were designed for each of the four groups; 29 out of 41 R
Molecular Plant-Microbe Interactions 10:550-559...Melanie J. Barnett 2 and Sharon R. Long 1 , 2...© 1997 The American Phytopathological Society...The Rhizobium meliloti SyrM protein activates transcription of nodD3 and syrA. Regulation of syrM is complex and may involve as yet undiscovered genes. Here we report the isolation of insertion mutants showing increased expression of a syrM-gusA gene fusion. Characterization of one mutant strain, d...
Biological dinitrogen fixation by Rhizobium spp. in the root nodules of leguminous plants such as soybean is of considerable agronomic importance. Biological dinitrogen fixation is ATP- and reductant-dependent; between 12 and 30 mol of ATP are required per mol of dinitrogen reduced [1]. All free-living rhizobia are aerobic although some strains will also grow anaerobically with nitrate as the terminal electron acceptor [2] ; ATP and reductant are generated during the oxidation of an exogenously supplied carbon source. In the bacteroids within the root nodule the exogenous carbon source (photosynthate) is derived from photosynthetic CO₂ fixation by the host plant. The identity of the carbon source(s) oxidised by the bacteroids in vivo has not yet been confirmed although sucrose is the major photosynthetic product translocated to the root nodules [3]. As dinitrogen fixation represents a drain on the photosynthetic supply [4,5] and since the supply of photosynthate is probably one of the major ...
Symbiotic nitrogen fixation of Rhizobia with leguminous crops accounts for 20% of the global nitrogen cycle. Rhizobium inoculation is always needed when certain new leguminous crops are introduced to new areas or regions. In addition to supply nitrogen to leguminous crops, they also spare soil nitrogen to succeeding crops. Thus the legume fixed nitrogen is important in sustaining pulse production.
Putative Rhizobium etli bv. phaseoli IE4803 RM systems. Sequence name: chromosome RetIE4803. GenBank: CP007641 (4,598,466 bp). ...
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
The X-ray crystal structure of the apo-form of the Fur protein from Rhizobium leguminosarum has been solved at 2.7 å resolution. Small-angle X-ray scattering was used to give information on the solution conformation of the protein. The Fur homodimer folds into two domains. The N-terminal domain is formed from the packing of two helix-turn-helix motifs while the C-terminal domain appears primarily to stabilize the dimeric state of the protein. ...
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dead zones. Toxic algae fills these dead zones, taking up oxygen and killing fish and plants in the process.. In charge of the BYU study is Paul Skip Price, a postdoctoral fellow at BYU. He and his team have found that rhizobia bacteria might be the key in allowing plants to process nitrogen naturally, without nitrogen-based fertilizers.. Price said about 80 percent of the atmosphere is nitrogen, but it is in a form that plants cannot use. However, rhizobia and plants can work together to become mutually beneficial, a process known as symbiosis.. By doing this and providing nitrogen naturally, you wont have to add more fertilizers which cause more runoff, Price said.. The objective of Prices study is to discover the types of rhizobia that will work in conjunction with plants.. One of the problems we have now is we dont know what makes a good rhizobia for the soil, Price said. And so thats what were studying, is how to improve the rhizobia for the plant.. Price has discovered that ...
ID C6B1I1_RHILS Unreviewed; 387 AA. AC C6B1I1; DT 01-SEP-2009, integrated into UniProtKB/TrEMBL. DT 01-SEP-2009, sequence version 1. DT 08-MAY-2019, entry version 63. DE SubName: Full=Binding-protein-dependent transport systems inner membrane component {ECO:0000313,EMBL:ACS54700.1}; GN OrderedLocusNames=Rleg_0389 {ECO:0000313,EMBL:ACS54700.1}; OS Rhizobium leguminosarum bv. trifolii (strain WSM1325). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium. OX NCBI_TaxID=395491 {ECO:0000313,EMBL:ACS54700.1, ECO:0000313,Proteomes:UP000002256}; RN [1] {ECO:0000313,EMBL:ACS54700.1, ECO:0000313,Proteomes:UP000002256} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=WSM1325 {ECO:0000313,EMBL:ACS54700.1, RC ECO:0000313,Proteomes:UP000002256}; RX PubMed=21304718; RA Reeve W., OHara G., Chain P., Ardley J., Brau L., Nandesena K., RA Tiwari R., Copeland A., Nolan M., Han C., Brettin T., Land M., RA Ovchinikova G., Ivanova N., ...
ID C6B1D9_RHILS Unreviewed; 466 AA. AC C6B1D9; DT 01-SEP-2009, integrated into UniProtKB/TrEMBL. DT 01-SEP-2009, sequence version 1. DT 07-JUN-2017, entry version 59. DE SubName: Full=ATPase, FliI/YscN family {ECO:0000313,EMBL:ACS54658.1}; DE EC=3.6.3.14 {ECO:0000313,EMBL:ACS54658.1}; GN OrderedLocusNames=Rleg_0347 {ECO:0000313,EMBL:ACS54658.1}; OS Rhizobium leguminosarum bv. trifolii (strain WSM1325). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium. OX NCBI_TaxID=395491 {ECO:0000313,EMBL:ACS54658.1, ECO:0000313,Proteomes:UP000002256}; RN [1] {ECO:0000313,EMBL:ACS54658.1, ECO:0000313,Proteomes:UP000002256} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=WSM1325 {ECO:0000313,EMBL:ACS54658.1, RC ECO:0000313,Proteomes:UP000002256}; RX PubMed=21304718; RA Reeve W., OHara G., Chain P., Ardley J., Brau L., Nandesena K., RA Tiwari R., Copeland A., Nolan M., Han C., Brettin T., Land M., RA Ovchinikova G., Ivanova N., ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; Rhizobium etli; Rhizobium etli bv. ...
For most of us, our closest encounter with the element fluorine is likely to be our toothpaste or a municipal water supply with added fluoride.. But excess fluorine can be a problem. For example, high levels of fluorine in the soil can hurt plants. Fluorine in soils may also affect microbes and other organisms higher along the food chain.. A new study explored whether soil fluorine levels in New Zealand are high enough to hurt a specific microbe called Rhizobium.. Rhizobium bacteria live in root nodules of legume plants, like beans and lentils. These bacteria fix atmospheric nitrogen, making the nutrients into a form the host plant can use.. Nitrogen fixation by Rhizobia means farmers need to use less nitrogen fertilizer. That can save significant costs.. If soil fluorine levels become high enough to hurt Rhizobia, it could impact the legume crops the bacteria help support.. In addition, pastures for grazing livestock often contain clover, another legume. High fluorine levels could harm ...
You man continents john alexander gilliard 2 she lanatomiste on and focus. Rather, its a trial that will gauge the impact of supporting the custom rom community this way. The advantages of referendums include the direct democratic element of them, the constitutional check they provide on a government, how they stimulate interest and involvement in public policy, provide a single, clear answer to a specific question in a way general elections cannot, and force policy makers to explain their proposals. Nursery stock is dipped in older man younger woman dating site a suspension of commercially prepared agrobacterium radiobacter strain 84 at planting time. Each and every one of us 60s and older senior online dating services is guided by the ideal of true sportsmanship. I hate you and stoned screeching faces maybe thats why they have dreadlocks. Kopp said the concept is where to meet persian singles in ny free suddenly becoming popular in china. There are wiper loose and combined with the usual ...
4KAD: Crystal structure of probable sugar kinase protein from Rhizobium etli CFN 42 complexed with N1-(2.3-dihydro-1H-inden-5-yl)acetam
TY - ABST. T1 - ¤In vivo¤ studies of nitrogen fixation and assimilation in pea-¤Rhizobium¤ nodules by 15N NMR spectroscopy. AU - Scharff, A.M.. AU - Hansen, P.E.. AU - Rosendahl, L.. N1 - Conference code: 3. PY - 1998. Y1 - 1998. KW - Planteproduktion og stofomsætning. M3 - Conference abstract for conference. T2 - 3rd European Nitrogen Fixation Conference. Y2 - 20 September 1998 through 24 September 1998. ER - ...
Extension of the current 12-month expiry of rhizobial inoculants in Australia to 18 months would have commercial benefits for the manufacturers and resellers. The dilemma, however, is that numbers of rhizobia in the inoculants decline over time and individual cells may lose efficacy. The research undertaken in this study shows the effect of lupin and chickpea inoculant age (i.e. 0, 6, 12, 15 and 18 months old) on numbers of rhizobia, rhizobial cell characteristics and efficacy. For the latter, assessments included colony size on plates, survival on inoculated beads, and infectiveness and effectiveness in field experiments at 3 sites.. Assessment of commercially produced inoculants at the Australian Legume Inoculants Research Unit (ALIRU) laboratory indicated that, on average, chickpea and lupin inoculants had counts of about log10 9.6 when fresh, delivering ,log10 6 rhizobia/seed. At 12 months, the average counts had fallen to log10 9.4, delivering slightly less than log10 6 rhizobia/seed. By 18 ...
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Molecular Plant-Microbe Interactions 15:350-359...Elizabeth A. Rathbun , Michael J. Naldrett , and Nicholas J. Brewin...© 2002 The American Phytopathological Society...Rhizobium leguminosarum bv. viciae normally gains access to pea host cells through tubular cell wall ingrowths termed infection threads. Matrix glycoprotein (MGP), a major component of the infection thread lumen, is also secreted from the tips of uninoculated roots and can be released into solution...
Biological nitrogen fixation (BNF) by plants and its bacterial associations represent an important natural system for capturing atmospheric dinitrogen (N2) and processing it into a reactive form of nitrogen through enzymatic reduction. The study of BNF in non-leguminous plants has been difficult compared to nodule-localized BNF in leguminous plants because of the diverse sites of N2 fixation in non-leguminous plants. Identification of the involved N2-fixing bacteria has also been difficult because the major nitrogen fixers were often lost during isolation attempts. The past 20 years of molecular analyses has led to the identification of N2 fixation sites and active nitrogen fixers in tissues and the rhizosphere of non-leguminous plants. Here, we examined BNF hotspots in six reported non-leguminous plants. Novel rhizobia and methanotrophs were found to be abundantly present in the free-living state at sites where carbon and energy sources were predominantly available. In the carbon-rich apoplasts of
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Name: Rhizobium miluonense Gu et al. 2008. Category: Species. Proposed as: sp. nov.. Etymology: mi.lu.o.nense N.L. neut. adj. miluonense, pertaining to the Miluo River, a famous river located in Hunan Province, where the bacterium was isolated Gender: neuter Type strains: CCBAU 41251; DSM 21815; HAMBI 2971; LMG 24208 See detailed strain information at ...
We have identified quantitative trait loci (QTL) for transgenic and adventitious root production using an Agrobacterium rhizogenes-mediated co-transformati
ID ARLY_AGRVS Reviewed; 467 AA. AC B9JTJ2; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 24-MAR-2009, sequence version 1. DT 25-OCT-2017, entry version 54. DE RecName: Full=Argininosuccinate lyase {ECO:0000255,HAMAP-Rule:MF_00006}; DE Short=ASAL {ECO:0000255,HAMAP-Rule:MF_00006}; DE EC=4.3.2.1 {ECO:0000255,HAMAP-Rule:MF_00006}; DE AltName: Full=Arginosuccinase {ECO:0000255,HAMAP-Rule:MF_00006}; GN Name=argH {ECO:0000255,HAMAP-Rule:MF_00006}; GN OrderedLocusNames=Avi_4017; OS Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis OS (strain S4)). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium. OX NCBI_TaxID=311402; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S4 / ATCC BAA-846; RX PubMed=19251847; DOI=10.1128/JB.01779-08; RA Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K., RA Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., RA Otten L., Suen G., ...
Copper effect on photosynthetic performance, symbiotic efficiency and biosorption of rhizobia associated with Horse gram [Macrotyloma uniflorum (Lam.) Verdc.]
A sand pouch-plant infection technique for counting most probable numbers of rhizobia in soil is described. Populations of Rhizobium leguminosarum bv. trifoli detected by the plant infection method performed in enclosed glass tubes or in sand pouches did not differ significantly. The described method was used to assess numbers of R. leguminosarum bv. trifoli (R.l.t.) and bv. viciae (R.l.v.) in 20 ...
A sand pouch-plant infection technique for counting most probable numbers of rhizobia in soil is described. Populations of Rhizobium leguminosarum bv. trifoli detected by the plant infection method performed in enclosed glass tubes or in sand pouches did not differ significantly. The described method was used to assess numbers of R. leguminosarum bv. trifoli (R.l.t.) and bv. viciae (R.l.v.) in 20 ...
2017 Rhizobium alamii Berge et al. 2009 "Rhizobium album" Hang et al. 2019 "Rhizobium albus" Li et al. 2017 Rhizobium altiplani ... 2016 Rhizobium alvei Sheu et al. 2015 Rhizobium anhuiense Zhang et al. 2015 Rhizobium aquaticum Máthé et al. 2019 "Rhizobium ... 2021 Rhizobium freirei Dall'Agnol et al. 2013 Rhizobium gallicum Amarger et al. 1997 Rhizobium gei Shi et al. 2016 "Rhizobium ... 1984 Rhizobium helianthi Wei et al. 2015 Rhizobium hidalgonense Yan et al. 2020 "Rhizobium indicum" Rahi et al. 2020 Rhizobium ...
... are rod-shaped bacteria found in the soil. They require oxygen and do not form spores. Rhizobium lentis grow ... Rhizobium lentis is genetically very closely related to the related species Rhizobium etli and Rhizobum phaseoli. The GC- ... nov., Rhizobium bangladeshense sp. nov. And Rhizobium binae sp. nov. From lentil (Lens culinaris) nodules". International ... Rhizobium lentis can utilize α-D lactose, β-methyl-D-glucoside, D-sorbitol, D-mannito, D-arbitol-glycerol, D-fructose-6- ...
245-. ISBN 978-3-642-05076-3. "Rhizobium indigoferae" at the Encyclopedia of Life LPSN Type strain of Rhizobium indigoferae at ... Rhizobium indigoferae is a Gram negative root nodule bacteria, which nodulates and forms nitrogen-fixing symbioses with ... Its type strain is CCBAU 71714(T) (= AS 1.3046(T)). Wei, G. H. (2002). "Rhizobium indigoferae sp. nov. and Sinorhizobium ... Phenotypic and Genotypic Diversity of Rhizobia. Bentham Science Publishers, 2012. Goyal, Aakash, and Priti Maheshwari, eds. ...
"Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov". Int J Syst ... "Rhizobium etli". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved May 28, 2021.{{cite web}}: CS1 maint ... Rhizobium etli is a Gram-negative root-nodule bacterium. Segovia L, Young JP, Martínez-Romero E. (1993). " ...
"Rhizobium lusitanum" at the Encyclopedia of Life LPSN Type strain of Rhizobium lusitanum at BacDive - the Bacterial Diversity ... Rhizobium lusitanum is a Gram negative root nodule bacteria, specifically nodulating Phaseolus vulgaris. Its type strain is P1- ... Phenotypic and Genotypic Diversity of Rhizobia. Bentham Science Publishers, 2012. " ... 7T (=LMG 22705T =CECT 7016T). Valverde, A.; Igual, J. M.; Peix, A.; Cervantes, E.; Velazquez, E. (2006). "Rhizobium lusitanum ...
... (formerly Agrobacterium rhizogenes) is a Gram-negative soil bacterium that produces hairy root disease in ... Young JM, Kuykendall LD, Martínez-Romero E, Kerr A, Sawada H (2001). "A revision of Rhizobium Frank 1889, with an emended ... 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola, and R. vitis". Int J Syst Evol Microbiol ...
... Dangeard 1926AL and Rhizobium trifolii Dangeard 1926AL. R. trifolii is a later synonym of R. leguminosarum. ... "Rhizobium phaseoli". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved May 28, 2021.{{cite web}}: CS1 ... Rhizobium phaseoli is a root nodule bacterium. Dangeard PA. (1926). "Recherches sur les tubercles radicaux des Légumineuses". ... Reclassification of the strain R. leguminosarum DSM 30132 (=NCIMB 11478) as Rhizobium pisi sp. nov". Int J Syst Evol Microbiol ...
... is a bacterium species from the genus Rhizobium which has been isolated from soil from the city airport in ... Rhizobium viscosum produces exopolysaccharide. Quintelas, C; Pereira, R; Kaplan, E; Tavares, T (August 2013). "Removal of Ni(II ... "Reclassification of Arthrobacter viscosus as Rhizobium viscosum comb. nov". Int J Syst Evol Microbiol. 67: 1789-1792. doi: ...
... is a root nodule bacterium. Martínez-Romero E, Segovia L, Mercante FM, Franco AA, Graham P, Pardo MA. (1991 ... "Rhizobium tropici". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved May 28, 2021.{{cite web}}: CS1 ... "Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees". Int J Syst Bacteriol. 41 (3 ...
... are rod-shaped bacteria found in the soil. They require oxygen and do not form spores. Rhizobium binae grow ... nov., Rhizobium bangladeshense sp. nov. And Rhizobium binae sp. nov. From lentil (Lens culinaris) nodules". International ... Rhizobium binae was first described in 2015 by M. Harun-or Rashid and others. It was isolated from the root nodules of Lens ... Rhizobium binae can grow in the presence of the antibiotic compounds lincomycin and potassium tellurite, but not in the ...
Amarger N, Macheret V, Laguerre G (October 1997). "Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus ... Silva, C.; Vinuesa, P.; Eguiarte, L. E.; Martínez-Romero, E.; Souza, V. (2003). "Rhizobium etli and Rhizobium gallicum Nodulate ... Amarger, N.; Macheret, V.; Laguerre, G. (1997). "Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus ... Shamseldin, Abdelaal; Werner, Dietrich (2006). "Presence of Rhizobium etli bv. phaseoli and Rhizobium gallicum bv. gallicum in ...
ISBN 978-1-4020-4019-1. "Rhizobium mongolense" at the Encyclopedia of Life LPSN Type strain of Rhizobium mongolense at BacDive ... Rhizobium mongolense is a Gram negative root nodule bacteria, which nodulates and forms nitrogen-fixing symbioses with Medicago ... van Berkum, P.; Beyene, D.; Bao, G.; Campbell, T. A.; Eardly, B. D. (1998). "Rhizobium mongolense sp. nov. is one of three ... Phosphate solubilizing rhizobia originating from Medicago, Melilotus and Trigonella grown in a Spanish soil. First ...
... is a Gram-negative, motile, rod-shaped, aerobic bacterium. Rhizobium leguminosarum biovar trifolii, and ... Rhizobium leguminosarum's acyl carrier protein differs from most ACPs by having a C-terminus extension. This ACP is also used ... Rhizobium leguminosarum is a bacterium which lives in a mutualistic symbiotic relationship with legumes, and has the ability to ... Type strain of Rhizobium leguminosarum at BacDive - the Bacterial Diversity Metadatabase v t e (Articles with short description ...
Rhizobium phaseoli Dangeard 1926AL and Rhizobium trifolii Dangeard 1926AL. R. trifolii is a later synonym of R. leguminosarum. ... "Rhizobium pisi sv. trifolii K3.22 harboring nod genes of the Rhizobium leguminosarum sv. trifolii cluster". Systematic and ... Rhizobium pisi is a root nodule bacterium. Ramírez-Bahena MH, García-Fraile P, Peix A, Valverde A, Rivas R, Igual JM, Mateos PF ... "Rhizobium pisi". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved May 28, 2021.{{cite web}}: CS1 maint ...
"Rhizobium hainanense" at the Encyclopedia of Life LPSN Type strain of Rhizobium hainanense at BacDive - the Bacterial Diversity ... Rhizobium hainanense is a Gram negative root nodule bacteria. Strain CCBAU 57015 (166) is the type strain. Chen, W.-X.; Tan, Z ... ISBN 0-7923-5180-0. Young JM, Park DC, Weir BS (September 2004). "Diversity of 16S rDNA sequences of Rhizobium spp. ... Dhabhai, Kshipra; Batra, Amla (2012). "Physiological and phylogenetic analysis of rhizobia isolated from Acacia nilotica L.". ...
... is a gram-negative bacterium which was isolated from root nodules of lentils in Bangladesh. Rhizobium ... nov., Rhizobium bangladeshense sp. nov. And Rhizobium binae sp. nov. From lentil (Lens culinaris) nodules". International ... Genetic analysis of R. bangladeshense has shown it to be most closely related to Rhizobium etli and Rhizobium phaseoli. The DNA ... Rashid, M.H., Gonzalez, H., Young, J.P.W., and Wink, M. (2014) Rhizobium leguminosarum is the symbiont of lentil in the Middle ...
... are a "group of soil bacteria that infect the roots of legumes to form root nodules". Rhizobia are found in the soil ... The legume-rhizobium symbiosis is a classic example of mutualism-rhizobia supply ammonia or amino acids to the plant and in ... "Current taxonomy of rhizobia". Archived from the original on 2013-06-04. Retrieved 2013-12-02. "Bacteria confused with rhizobia ... Legume sanctions maintain Rhizobium mutualism Current list of rhizobia species Nitrogen Fixation and Inoculation of Forage ...
... (EC 2.5.1.98, PssM) is an enzyme with systematic ... Rhizobium+leguminosarum+exopolysaccharide+glucosyl+ketal-pyruvate-transferase at the US National Library of Medicine Medical ... "Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum ... for pyruvylation of subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium ...
The rhizobia and their hosts must be able to recognize each other for nodule formation to commence. Rhizobia are specific to ... These bacteria, known as rhizobia, have the ability to take nitrogen gas (N2) out of the air and convert it to a form of ... It is found in the majority of its members that only form an association with rhizobia, which in turn form an exclusive ... The legume, acting as a host, and rhizobia, acting as a provider of usable nitrate, form a symbiotic relationship. Members of ...
If rhizobia are present, root nodulation begins by the time the third node appears. Nodulation typically continues for 8 weeks ... Rhizobium japonicum; Jordan 1982). This ability to fix nitrogen allows farmers to reduce nitrogen fertilizer use and increase ... Like many legumes, soybeans can fix atmospheric nitrogen, due to the presence of symbiotic bacteria from the Rhizobia group. ...
Raina JL, Modi VV (August 1972). "Deoxyribonucleate binding and transformation in Rhizobium jpaonicum". Journal of Bacteriology ... Rhizobium spp.), endosymbionts of arthropods (Wolbachia) and intracellular pathogens (e.g. Rickettsia). Moreover, the class is ... A species of technological interest is Rhizobium radiobacter (formerly Agrobacterium tumefaciens): scientists often use this ...
Chen WX, Yan GH, Li JL (1988). "Numerical taxonomic study of fast-growing soybean rhizobia and a proposal that Rhizobium fredii ... "a Rhizobium isolated from China", in turn referring to the related genus Rhizobium ("root-associated life form"). The name ... February 9, 2013). "Rhizobium: Writing about bacteria and their genomes". WordPress. Wang ET, Tan ZY, Willems A, Fernández- ... Wei GH, Wang ET, Tan ZY, Zhu ME, Chen WX (November 2002). "Rhizobium indigoferae sp. nov. and Sinorhizobium kummerowiae sp. nov ...
Ghosh, Pallab Kumar; Maiti, Tushar Kanti (2016). "Structure of Extracellular Polysaccharides (EPS) Produced by Rhizobia and ... myxogenes) cyclosophorans (Agrobacterium spp., Rhizobium spp. and Xanthomonas spp.) dextran (Leuconostoc mesenteroides, ... Rhizobium spp. and Zooglea' spp.) galactosaminogalactan (Aspergillus spp.) gellan (Aureomonas elodea and Sphingomonas ...
In some rhizobia, the nif genes are located on plasmids called 'sym plasmids' (sym = symbiosis) which contain genes related to ... Rhizobium spp.-Gram-negative, symbiotic nitrogen fixing bacteria that usually form a symbiotic relationship with legume species ... The first Rhizobium genes for nitrogen fixation (nif) and for nodulation (nod) were cloned in the early 1980s by Gary Ruvkun ...
"Rhizobium rosettiformans sp. nov., isolated from a hexachlorocyclohexane dump site, and reclassification of Blastobacter ... aggregatus Hirsch and Muller 1986 as Rhizobium aggregatum comb. nov". Int J Syst Evol Microbiol. 61 (Pt 5): 1218-1225. doi: ...
Scholla, M. H.; Elkan, G. H. (1984). "Rhizobium fredii sp. nov., a Fast-Growing Species That Effectively Nodulates Soybeans". ... Sadowsky MJ, Cregan PB, Keyser HH (August 1988). "Nodulation and Nitrogen Fixation Efficacy of Rhizobium fredii with Phaseolus ... Balatti PA, Pueppke SG (December 1990). "Cultivar-Specific Interactions of Soybean with Rhizobium fredii Are Regulated by the ... Forsberg LS, Reuhs BL (September 1997). "Structural characterization of the K antigens from Rhizobium fredii USDA257: evidence ...
"Rhizobium huautlense" at the Encyclopedia of Life LPSN v t e (Articles with short description, Short description matches ... 31 July 2014). "Rhizobium smilacinae sp. nov., an endophytic bacterium isolated from the leaf of Smilacina japonica". Antonie ... Wang, E. T.; van Berkum, P.; Beyene, D.; Sui, X. H.; Dorado, O.; Chen, W. X.; Martinez-Romero, E. (1998). "Rhizobium huautlense ... nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae". International Journal ...
Amarger, N.; Macheret, V.; Laguerre, G. (1997). "Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus ... Beyhaut, Elena; Tlusty, Becki; van Berkum, Peter; Graham, Peter H (2006). "Rhizobium giardinii is the microsymbiont of Illinois ... Mhamdi, R (2002). "Different species and symbiotic genotypes of field rhizobia can nodulate Phaseolus vulgaris in Tunisian ... "A new taxonomic marker of nodule bacteria of the Rhizobium genus and its evolution". Russian Journal of Genetics: Applied ...
and Krsmanovi-Simic D, Werquin M (1973). "Etude des bactériophages de Rhizobium meliloti" [Study of bacteriophages of Rhizobium ... Krsmanovi-Simic D, Werquin M (1977). "Etude des bactériophages de Rhizobium meliloti" [Study of bacteriophages of Rhizobium ... interacts with the free oxygen in the root nodule where the rhizobia reside. Rhizobia are contained within symbiosomes in the ... The rhizobia then penetrate the root hairs and proliferate to form an infection thread. Through the infection thread, the ...
... and Rhizobium sp. The Indole test is one of the four tests of the IMViC series, which tests for evidence of an enteric ...
1.3 mV on legume-Rhizobium symbiosis, which largely determines the efficiency of soil nitrogen assimilation and the yield of ... for the observed decrease in the activity of peroxidase and polyphenol oxidase in parts of plant roots susceptible to rhizobia ... Rhizobium symbiosis, the so-called rhizobia are formed in the parenchymal tissue of the root nodules in which rhizobia, being ... Rhizobium symbiosis [3]. Legume-Rhizobium symbiosis is of particular importance for leguminous plants (although not strictly ...
Stabiliteit van knopvormingsvermoe van Rhizobium japonicum. Main Author:. Van Rensburg, Henri Jansen.. Format:. Book. Language: ...
... particles of two Rhizobium bacteriophages interfered temporarily with the multiplication of active particles of the homologous ... Kleczkowska L. 1945; The production of plaques by Rhizobiumbacteriophage in poured plates and its value as a counting method. J ... The Behaviour of Rhizobium Bacteriophages during and after Exposure to Ultraviolet Radiation * J. Kleczkowski1, A. Kleczkowski1 ... The Behaviour of Rhizobium Bacteriophages during and after Exposure to Ultraviolet Radiation ...
Rhizobium meliloti Exopolysaccharides: Structures, Genetic Analyses, and Symbiotic Roles. Annals of the New York Academy of ... Rhizobium meliloti Exopolysaccharides : Structures, Genetic Analyses, and Symbiotic Roles. In: Annals of the New York Academy ... Rhizobium meliloti Exopolysaccharides: Structures, Genetic Analyses, and Symbiotic Roles. T. L. REUBER, A. URZAINQUI, J. ... Rhizobium meliloti Exopolysaccharides : Structures, Genetic Analyses, and Symbiotic Roles. / REUBER, T. L.; URZAINQUI, A.; ...
A vitamin B12 dependent mutualism between a microalga Lobomonas rostrata and a rhizobium - Mesorhizobium loti. Add to your list ... A vitamin B12 dependent mutualism between a microalga Lobomonas rostrata and a rhizobium - Mesorhizobium loti ...
Thus, the objective of this study was to evaluate the effect of inoculation with rhizobia strains in compacted soil on the ... and the combination of the strains MT8 - Rhizobium tropici and MT15 - R. tropici) and a control with nitrogen fertilization - ... and the combination of the strains MT8 - Rhizobium tropici and MT15 - R. tropici) and a control with nitrogen fertilization - ... Thus, the objective of this study was to evaluate the effect of inoculation with rhizobia strains in compacted soil on the ...
Transcriptomic analysis of Rhizobium leguminosarum biovar viciae in symbiosis with host plants Pisum sativum and Vicia cracca. ... Transcriptomic analysis of Rhizobium leguminosarum biovar viciae in symbiosis with host plants Pisum sativum and Vicia cracca. ...
The legume is known for its high promiscuity in nodulating with several Rhizobium species, but those belonging to the Rhizobium ... Genome of Rhizobium leucaenae strains CFN 299T and CPAO 29.8: searching for genes related to a successful symbiotic performance ... Genome of Rhizobium leucaenae strains CFN 299T and CPAO 29.8: searching for genes related to a successful symbiotic performance ... Rhizobium leucaenae belongs to this group, which is abundant in the Brazilian Cerrados soils and frequently submitted to ...
Phenotypic characterization of this new desert rhizobium species showed that it was capable to utilize malonate, to grow at 48& ... From: Genomic characterization of Ensifer aridi, a proposed new species of nitrogen-fixing rhizobium recovered from Asian, ...
Kittiwongwattana C, Thawai C. Rhizobium paknamense sp. nov., isolated from lesser duckweeds (Lemna aequinoctialis). Int J Syst ...
Centre for Rhizobium Studies. School of Veterinary and Life Sciences. Publisher:. Nature Publishing Group, a division of ... Rhizobia must exist in soil and compete with other members of the microbiota before infecting legumes and forming N2-fixing ... Rhizobia are some of the best-studied plant microbiota. These oligotrophic Alphaproteobacteria or Betaproteobacteria form ... 2018) Rhizobia: from saprophytes to endosymbionts. Nature Reviews Microbiology, 16 . pp. 291-303. ...
Parent taxon: Rhizobium Frank 1889 (Approved Lists 1980) Assigned by: Greub G, La Scola B, Raoult D. Amoebae-resisting bacteria ... "Candidatus Rhizobium massiliae" Greub et al. 2004 other spelling of name, inaccurate spelling. ... Linking: To permanently link to this page, use https://lpsn.dsmz.de/species/rhizobium-massiliense. Copy to clipboard. Link ...
It was concluded that symbiotic rhizobia introduced by the inoculant were more efficient than free-living rhizobia in soils ... Most Rhizobium was detected between a SOC content of 2.03% to 3.80% in both inoculated and non-inoculated soils. Inoculation ... The effect of soil carbon on symboitic nitrogen fixation and symbiotic Rhizobium populations in soil with Trifolium repens as ... The effect of soil carbon on symboitic nitrogen fixation and symbiotic Rhizobium populations in soil with Trifolium repens as ...
Bean-nodulating Rhizobium etli originated in Mesoamerica, while soybean-nodulating Sinorhizobium fredii evolved in East Asia. S ... From: The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium ...
... japonicum and cowpea rhizobia under desiccation. As one would expect, variation exists among Rhizobium spp. as well as ... Drought Impact on Soil Rhizobia Populations December 19, 2012. /0 Comments/in Uncategorized /by shawn conley [email protected] ... The soybean plant provides nutrients and a protective growing environment for the rhizobia. In turn, the rhizobia "fix" ... You are here: Home / Blog / Uncategorized / Drought Impact on Soil Rhizobia Populations ...
Catalogue » Bacteria » Proteobacteria » Alphaproteobacteria » Rhizobiales » Rhizobiaceae » Rhizobium » Rhizobium radiobacter ( ... Rhizobium radiobacter (Beijerinck & van Delden, 1902, 2001) (Traces of animal activity) Zoja (Zoja Berest) 08.09.2022 12:16:41 ...
Take Rhizobium in a tray (@ 25g /1kg of seeds), add the wet seeds to this, and gently mix by rolling the seeds in the powder so ... Rhizobium is available as a wet powder formulation only. DOSAGE & METHOD OF APPLICATION: Direct Application: Apply 20 g to ... Bazodo Rhizobium is a biofertilizer containing a symbiotic nitrogen-fixing organism of leguminous plants. ... Rhizobium is a soil microbe that lives in symbiotic association with leguminous plants. Rhizobium colonizes the root nodules of ...
In the present study, we demonstrate that the growth of salt-stressed pepper plants is improved by inoculation with plant growth… ...
AGTIV FUEL inoculants with rhizobium FUEL soybean crops by fixing nitrogen for better growth. ... RHIZOBIUM - PTB162 Technology. Bradyrhizobium japonicum: 1.1 x 108 viable cells per gram ... AGTIV® FUEL™ inoculants with rhizobium FUEL soybeans by fixing nitrogen for better growth. ...
Isolation & characterization of antibiotic resistant mutants of cowpea Rhizobium 32Hi.. Authors: Dhar, B. Singh, B D. Singh, R ... Isolation & characterization of antibiotic resistant mutants of cowpea Rhizobium 32Hi. Indian Journal of Experimental Biology. ...
This site is also protected by an SSL (Secure Sockets Layer) certificate thats been signed by the U.S. government. The https:// means all transmitted data is encrypted - in other words, any information or browsing history that you provide is transmitted securely.. ...
This site is also protected by an SSL (Secure Sockets Layer) certificate thats been signed by the U.S. government. The https:// means all transmitted data is encrypted - in other words, any information or browsing history that you provide is transmitted securely.. ...
Rhizobium vitis strain VAT03-9 (MAFF 211676) is a causal agent of crown gall disease in grapevine. It is one of the pathogenic ... N2 - Rhizobium vitis strain VAT03-9 (MAFF 211676) is a causal agent of crown gall disease in grapevine. It is one of the ... AB - Rhizobium vitis strain VAT03-9 (MAFF 211676) is a causal agent of crown gall disease in grapevine. It is one of the ... abstract = "Rhizobium vitis strain VAT03-9 (MAFF 211676) is a causal agent of crown gall disease in grapevine. It is one of the ...
Ecological Survey and Conservation of Legume-Symbiotic Rhizobia Genetic Diversity in Papua New Guinea, 2004. Author Tomooka N. ... Ecological Survey and Conservation of Legume-Symbiotic Rhizobia Genetic Diversity in Papua New Guinea, 2004 ...
In the present study, cross inoculation of Rhizobium aegy... ... In the present study, cross inoculation of Rhizobium ... Bouraoui M, Abbes Z, Rouissi M, Abdi N, Hemissi I, Kouki S, Sifi B (2016) Effect of rhizobia inoculation, N and P supply on ... Cross inoculation with beneficial Rhizobium strain promotes plant growth in Vigna mungo Shweta Chaudhary ... Youseif SH, Fayrouz H, El-Megeed A, Saleh SA (2017) Improvement of Faba Bean yield using Rhizobium/Agrobacterium inoculant in ...
A legume kinesin controls vacuole morphogenesis for rhizobia endosymbiosis This study uncovered a gene duplication in legumes ... that produces a nodule-enriched nKCBP protein, a plant-specific kinesin motor, and found that rhizobia co-opts nKCBP to control ...
Control of the initial steps in heme biosynthesis in free-living Rhizobium sp. by culture conditions ...
and Rhizobium spp. (6). Whether soil is the primary habitat of B. microti or other vectors, such as nematodes, remains to be ...
Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli ... The structure of Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli also contains other ... The structure of Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli, PDB code: 2qf7 was ... Magnesium in PDB 2qf7: Crystal Structure of A Complete Multifunctional Pyruvate Carboxylase From Rhizobium Etli. Enzymatic ...
  • Effect of Foliar Treatment with Aqueous Dispersions of Silver Nanoparticles on Legume-Rhizobium Symbiosis and Yield of Soybean (Glycine max L. Merr. (mdpi.com)
  • Transcriptomic analysis of Rhizobium leguminosarum biovar viciae in symbiosis with host plants Pisum sativum and Vicia cracca. (jic.ac.uk)
  • The application of rhizobium and mycorrhiza, is expected to occur tripartite beneficial symbiosis. (envirobiotechjournals.com)
  • I also spent a summer working in a plant development lab studying the genetics of the medicago trunctuala /rhizobia symbiosis. (lianaburghardtlab.com)
  • Thus, the objective of this study was to evaluate the effect of inoculation with rhizobia strains in compacted soil on the development and production of cowpea. (academicjournals.org)
  • and the combination of the strains MT8 - Rhizobium tropici and MT15 - R. tropici) and a control with nitrogen fertilization - 150 mg dm-3, using urea as a source, in compacted and uncompacted soil. (academicjournals.org)
  • In a parallel study Oso-Afiana and Alexander (1982) reported similar results when comparing strains of R. japonicum and cowpea rhizobia under desiccation. (coolbean.info)
  • This work aimed to evaluate the growth of Rhizobium tropici BR322 and BR 520 strains, used as inoculant on common bean in Brazil, in yeast extract manitol (YM) medium supplemented with different herbicides (bentazon, metolachlor, imazamox, fluazifop-p-butyl, fomesafen and paraquat). (ufv.br)
  • The first included 25 provenances of G. sepium inoculated with a mixture of three strains of Rhizobium spp. (iita.org)
  • The second experiment included six strains of Rhizobium spp. (iita.org)
  • The study concluded that five rhizobial isolates from Rwanda are highly effective on bush and climbing beans and compared well with rhizobia strains CIAT 899. (n2africa.org)
  • Distribution, Characterization and the Commercialization of Elite Rhizobia Strains in Africa. (bvsalud.org)
  • Although much research has been conducted on rhizobia in nitrogen fixation and their contribution to soil fertility , much less is known about the distribution and diversity of the bacteria strains in different areas of the world and which of the strains achieve optimal benefits for the host plants under specific soil and environmental conditions. (bvsalud.org)
  • SUMMARY: After inactivation by ultraviolet radiation, particles of two Rhizobium bacteriophages interfered temporarily with the multiplication of active particles of the homologous phage, in liquid cultures of their respective host bacteria. (microbiologyresearch.org)
  • Effect of specific polysaccharides produced by the host bacteria and of ribonuclease on the multiplication of Rhizobium phages. (microbiologyresearch.org)
  • Mutation of ptsP encoding EI(Ntr) of the PTS(Ntr) system in Rhizobium leguminosarum strain Rlv3841 caused a pleiotropic phenotype as observed with many bacteria. (ox.ac.uk)
  • NodO, a 30-kDa nodulation protein secreted by Rhizobium leguminosarum biovar viciae, belongs to a family of proteins produced by Gram-negative bacteria containing a variable number of glycine/aspartates nonapeptides. (ukhsa.gov.uk)
  • Rhizobia must exist in soil and compete with other members of the microbiota before infecting legumes and forming N2-fixing bacteroids. (edu.au)
  • The amount of nitrogen fixed by Rhizobium symbiotically with Trifolium repens (white clover) is ultimately determined by the quality of the soil environment. (up.ac.za)
  • Inoculation increased the number of rhizobia in soil. (up.ac.za)
  • 1995. Tolerance of clover rhizobia to heat and desiccation stresses in soil. (coolbean.info)
  • Rhizobium is a soil microbe that lives in symbiotic association with leguminous plants. (bazodo.com)
  • In the case of no rhizobium inoculation, soil N content in cocoa farm with A. pintoicover crop was lower than that of without cover crop or with C. caeruleum. (ccrjournal.com)
  • New paper: rhizobium evolution feeds back on soil nitrogen! (illinois.edu)
  • IMSEAR at SEARO: Isolation & characterization of antibiotic resistant mutants of cowpea Rhizobium 32Hi. (who.int)
  • The effect of SOC on the total number of symbiotic and saprophytic rhizobia was determined. (up.ac.za)
  • It was concluded that symbiotic rhizobia introduced by the inoculant were more efficient than free-living rhizobia in soils with higher carbon content, which highlights the importance of inoculation in improving the sustainable production of T. repens pastures. (up.ac.za)
  • Bazodo Rhizobium is a biofertilizer containing a symbiotic nitrogen-fixing organism of leguminous plants. (bazodo.com)
  • This study uncovered a gene duplication in legumes that produces a nodule-enriched nKCBP protein, a plant-specific kinesin motor, and found that rhizobia co-opts nKCBP to control vacuole morphogenesis in symbiotic cells, thus achieving successful endosymbiosis. (nature.com)
  • Phylogeography and also Symbiotic Success associated with Rhizobia Nodulating Chickpea (Cicer arietinum T.) inside Ethiopia. (p450inhibitors.com)
  • Improvement of legume-Rhizobium symbiotic nitrogen (N2) fixation has been a long-term goal for agricultural science. (usda.gov)
  • Rhizobium vitis strain VAT03-9 (MAFF 211676) is a causal agent of crown gall disease in grapevine. (elsevier.com)
  • In the present study, cross inoculation of Rhizobium aegyptiacum , an osmotolarant bacterial strain enhances growth in Vigna mungo cv. (vegetosindia.org)
  • Howieson J, Malden J, Yates R, Hara G (2000) Techniques for selection and development of elite inoculant strain of Rhizobium leguminosarum in South Australia. (vegetosindia.org)
  • Strain SP 14 to 71070 for Rhizobium SP 44 at the last harvest. (iita.org)
  • Iqbal R, Mahmood A (1992) Response of Leucaena leucocephala to inoculation with rhizobia from tropical legumes. (vegetosindia.org)
  • Mahmood A, Athar M (2008) Cross inoculation studies: response of Vigna mungo to inoculation with rhizobia from tree legumes growing under arid Environment. (vegetosindia.org)
  • The legume is known for its high promiscuity in nodulating with several Rhizobium species, but those belonging to the Rhizobium tropici 'group' are the most successful and efficient in fixing nitrogen in tropical acid soils. (altmetric.com)
  • O objetivo deste trabalho foi avaliar o crescimento das estirpes de Rhizobium tropici BR 322 e BR 520, utilizadas como inoculantes na cultura do feijoeiro no Brasil, em meio de cultura à base de manitol e extrato de levedura (YM) adicionado de diferentes herbicidas (bentazon, metolachlor, imazamox, fluazifop-p-butil, fomesafen e paraquat). (ufv.br)
  • Recent advances in whole genome sequencing of rhizobia (described elsewhere in this Congress) and in plant functional genomics offer new strategies and targets for improving N2 fixation. (usda.gov)
  • Structure and Evolution of NGRRS-1, a Complex, Repeated Element in the Genome of Rhizobium sp. (unige.ch)
  • Rhizobium colonizes the root nodules of legumes and fixes nitrogen utilizing the host carbohydrates as an energy source. (bazodo.com)
  • Peanuts including legumes that can interact with Rhizobium and mycorrhizae. (envirobiotechjournals.com)
  • Pena-Cabriales and Alexander (1979) reported a biphasic decline in Rhizobium japonicum numbers in soils undergoing drying. (coolbean.info)
  • Solaiman ARM, Rabbani MG (2004) Effects of Rhizobium inoculant and nitrogen application on pea. (vegetosindia.org)
  • The result showed application of mycorrhiza and rhizobium increases plant height and plant dry weight and application of inoculant mycorrhiza indigenous be single or double and mycorrhiza mycofer increase the percent of infection mycorrhiza. (envirobiotechjournals.com)
  • The study also include the ability of four local Rhizobium isolates to growth and survive in YEM with three sodium chloride concentration 0.002% (control), 1%, 5% for five period, zero, 1, 2, 3, 5 and 7 day after incubation. (edu.iq)
  • Generally the viable cells of these four Rhizobium isolates decrease with the increasing of NaCl to 1% and 5% in broth media. (edu.iq)
  • Greenhouse and field experiments were conducted to evaluate the effectiveness of rhizobia isolates from two Rwandan soils and their effectiveness on two types of common bean under different agro ecological zones. (n2africa.org)
  • A preliminary greenhouse experiment using Leonard Jars in Rubona Station was conducted to evaluate the potential of 174 rhizobia isolates. (n2africa.org)
  • Highly effective isolates of rhizobia for Common bean were identified through a stepwise approach. (n2africa.org)
  • The effectiveness of rhizobia isolates on two types of beans (bush bean (RWR 1667) and climbing bean (Gasilida) was conducted in a pot experiment using CIAT 889, UMR 1597 as standard for comparison and and nitrogen nutrient as control. (n2africa.org)
  • Rhizobium leucaenae belongs to this group, which is abundant in the Brazilian 'Cerrados' soils and frequently submitted to several environmental stresses. (altmetric.com)
  • Most Rhizobium was detected between a SOC content of 2.03% to 3.80% in both inoculated and non-inoculated soils. (up.ac.za)
  • 1982. Clays anmd the survival of rhizobium in soils during dessication. (coolbean.info)
  • AGTIV ® FUEL™ inoculants with rhizobium FUEL soybeans by fixing nitrogen for better growth. (ptagtiv.com)
  • Hossain D, Solaiman ARM (2004) Performance of mungbean varieties as affected by Rhizobium inoculants. (vegetosindia.org)
  • The present work seeks to perform an analysis of the combination of biostimulating microorganisms (mycorrhizae and Rhizobium), as well as alternatives that guarantee the sustainability of the agricultural production system in the zones. (utb.edu.ec)
  • Rhizobia are some of the best-studied plant microbiota. (edu.au)
  • The availability of a large body of genomic, physiological, biochemical and ecological studies makes rhizobia unique models for investigating community interactions and plant colonization. (edu.au)
  • The soybean plant provides nutrients and a protective growing environment for the rhizobia. (coolbean.info)
  • In turn, the rhizobia "fix" atmospheric nitrogen into ammonia (NH3), which can then be used by the soybean plant. (coolbean.info)
  • Cover crop increased plant N content when there was no inoculation, on the other hand rhizobium inoculation decreased N content of cocoa tissue. (ccrjournal.com)
  • Tissue P content of cocoa plant was not influenced by A. Pintoicover crop or by rhizobium inoculation, except that the P tissue content of cocoa was 28% higher when the cover crop was C. caeruleumand inoculated. (ccrjournal.com)
  • Enumeration of Soybean-Associated Rhizobia with Quantitative Real Time Polymerase Chain Reaction. (coolbean.info)
  • Variation in nodulation and N2 fixation by the Gliricidia sepium/Rhizobium spp. (iita.org)
  • Former MS student Alex Riley's paper on population genetic structure in rhizobium divided genomes is out in Molecular Ecology. (illinois.edu)
  • The seed-applied fungicides captan and thiram alter phenotypic characteristics of Rhizobium Leguminosrum bv. (canada.ca)
  • Rhizobium is available as a wet powder formulation only. (bazodo.com)
  • Take Rhizobium in a tray (@ 25g /1kg of seeds), add the wet seeds to this, and gently mix by rolling the seeds in the powder so that the seeds will be coated uniformly. (bazodo.com)
  • The herbicide concentration I50, (which decreased Rhizobium growth in 50%) could not be determined in the fomesafen concentrations tested, isolated or mixed to fluazifop-p-butyl. (ufv.br)
  • 2009). 'Isolation of a dapttable or tolerant rhizobium and test its growth and survival in different salt concentration', ANBAR JOURNAL OF AGRICULTURAL SCIENCES , 7(4), pp. 290-296. (edu.iq)
  • Nucleotide sequence and genetic structure of a novel carbaryl hydrolase gene (cehA) from Rhizobium sp. (ncku.edu.tw)
  • 深入研究「Nucleotide sequence and genetic structure of a novel carbaryl hydrolase gene (cehA) from Rhizobium sp. (ncku.edu.tw)
  • on the ecosystem-level impacts of rhizobium evolution, which just came out in Oecologia. (illinois.edu)
  • This experiment laid out in split-split plot design consisted of three levels of cover crop (without, A. pintoiand Calopogonium caeruleum), two levels of rhizobium inoculation (not inoculated and inoculated) and two levels of phosphorus application (no P added and P added). (ccrjournal.com)
  • The PTS(Ntr) system globally regulates ATP-dependent transporters in Rhizobium leguminosarum. (ox.ac.uk)
  • Further, it describes the new concept of rhizobia, the latest system. (bookshare.org)
  • Bouraoui M, Abbes Z, Rouissi M, Abdi N, Hemissi I, Kouki S, Sifi B (2016) Effect of rhizobia inoculation, N and P supply on Orobanche foetida parasitizing faba bean ( Vicia faba minor) under field conditions. (vegetosindia.org)