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ReticulocytesReticulocyte CountRabbitsProtein BiosynthesisCell-Free SystemPeptide Initiation FactorsErythrocyte CountErythropoiesisGlobinsErythrocyte AgingErythrocytesHeminPeptide Chain Initiation, TranslationalPhenylhydrazinesRibosomesAnemia, Sickle CellPolyribosomesRNA, MessengerHemoglobinsMolecular WeightTransferrinErythropoietinHemeReceptors, TransferrinErythroblastsEukaryotic Initiation Factor-2KineticsReticulocytosisFetal HemoglobinAnemiaUbiquitinsThalassemiaMicronuclei, Chromosome-DefectiveIronBlood Cell CountElectrophoresis, Polyacrylamide GelBlood ProteinsAnemia, HemolyticErythrocyte MembranePuromycinPeptide BiosynthesisRNA, Transfer, Amino AcylHeptanoatesHeinz BodiesCarcinoma, Krebs 2BloodlettingHemoglobins, AbnormalHematocritLipoxygenaseArachidonate 15-LipoxygenaseMethionineAnemia, HypochromicMolecular Sequence DataBase SequencePeptide Chain Elongation, TranslationalErythrocytes, AbnormalPoly AMicronucleus TestseIF-2 KinaseRNA, TransferCentrifugation, Density GradientErythrocyte IndicesSpherocytosis, HereditaryAmino Acid SequenceAurintricarboxylic AcidRNA, Transfer, MetPeptide HydrolasesErythroid Precursor CellsTriticumAnemia, MacrocyticRNA CapsProteinsRNAGuanosine TriphosphateAnemia, Hemolytic, AutoimmuneProtein KinasesIron IsotopesAdenosine TriphosphateHematologic TestsPeptide Elongation FactorsIron RadioisotopesHemoglobin, SickleMacromolecular SubstancesChromatography, DEAE-CelluloseRecombinant ProteinsAnemia, NeonatalHemolysisEncephalomyocarditis virusAnemia, Hemolytic, CongenitalErythrocyte VolumeEdeine
Reticulocytes
Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.
Reticulocyte Count
The number of RETICULOCYTES per unit volume of BLOOD. The values are expressed as a percentage of the ERYTHROCYTE COUNT or in the form of an index ("corrected reticulocyte index"), which attempts to account for the number of circulating erythrocytes.
Rabbits
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Protein Biosynthesis
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Cell-Free System
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Peptide Initiation Factors
Protein factors uniquely required during the initiation phase of protein synthesis in GENETIC TRANSLATION.
Erythrocyte Count
The number of RED BLOOD CELLS per unit volume in a sample of venous BLOOD.
Erythropoiesis
The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.
Globins
A superfamily of proteins containing the globin fold which is composed of 6-8 alpha helices arranged in a characterstic HEME enclosing structure.
Erythrocyte Aging
The senescence of RED BLOOD CELLS. Lacking the organelles that make protein synthesis possible, the mature erythrocyte is incapable of self-repair, reproduction, and carrying out certain functions performed by other cells. This limits the average life span of an erythrocyte to 120 days.
Erythrocytes
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Hemin
Chloro(7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-N(21),N(22),N(23),N(24)) ferrate(2-) dihydrogen.
Peptide Chain Initiation, Translational
A process of GENETIC TRANSLATION whereby the formation of a peptide chain is started. It includes assembly of the RIBOSOME components, the MESSENGER RNA coding for the polypeptide to be made, INITIATOR TRNA, and PEPTIDE INITIATION FACTORS; and placement of the first amino acid in the peptide chain. The details and components of this process are unique for prokaryotic protein biosynthesis and eukaryotic protein biosynthesis.
Phenylhydrazines
Diazo derivatives of aniline, used as a reagent for sugars, ketones, and aldehydes. (Dorland, 28th ed)
Ribosomes
Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.
Anemia, Sickle Cell
A disease characterized by chronic hemolytic anemia, episodic painful crises, and pathologic involvement of many organs. It is the clinical expression of homozygosity for hemoglobin S.
Polyribosomes
A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Hemoglobins
The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements.
Molecular Weight
The sum of the weight of all the atoms in a molecule.
Transferrin
An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.
Erythropoietin
Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
Heme
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
Receptors, Transferrin
Membrane glycoproteins found in high concentrations on iron-utilizing cells. They specifically bind iron-bearing transferrin, are endocytosed with its ligand and then returned to the cell surface where transferrin without its iron is released.
Erythroblasts
Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.
Eukaryotic Initiation Factor-2
Eukaryotic initiation factor of protein synthesis. In higher eukaryotes the factor consists of three subunits: alpha, beta, and gamma. As initiation proceeds, eIF-2 forms a ternary complex with Met-tRNAi and GTP.
Kinetics
The rate dynamics in chemical or physical systems.
Reticulocytosis
An increase in circulating RETICULOCYTES, which is among the simplest and most reliable signs of accelerated ERYTHROCYTE production. Reticulocytosis occurs during active BLOOD regeneration (stimulation of red bone marrow) and in certain types of ANEMIA, particularly CONGENITAL HEMOLYTIC ANEMIA.
Fetal Hemoglobin
The major component of hemoglobin in the fetus. This HEMOGLOBIN has two alpha and two gamma polypeptide subunits in comparison to normal adult hemoglobin, which has two alpha and two beta polypeptide subunits. Fetal hemoglobin concentrations can be elevated (usually above 0.5%) in children and adults affected by LEUKEMIA and several types of ANEMIA.
Anemia
A reduction in the number of circulating ERYTHROCYTES or in the quantity of HEMOGLOBIN.
Ubiquitins
A family of proteins that are structurally-related to Ubiquitin. Ubiquitins and ubiquitin-like proteins participate in diverse cellular functions, such as protein degradation and HEAT-SHOCK RESPONSE, by conjugation to other proteins.
Thalassemia
A group of hereditary hemolytic anemias in which there is decreased synthesis of one or more hemoglobin polypeptide chains. There are several genetic types with clinical pictures ranging from barely detectable hematologic abnormality to severe and fatal anemia.
Micronuclei, Chromosome-Defective
Defective nuclei produced during the TELOPHASE of MITOSIS or MEIOSIS by lagging CHROMOSOMES or chromosome fragments derived from spontaneous or experimentally induced chromosomal structural changes.
Iron
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
Blood Cell Count
The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.
Electrophoresis, Polyacrylamide Gel
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Blood Proteins
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Anemia, Hemolytic
A condition of inadequate circulating red blood cells (ANEMIA) or insufficient HEMOGLOBIN due to premature destruction of red blood cells (ERYTHROCYTES).
Erythrocyte Membrane
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
Puromycin
A cinnamamido ADENOSINE found in STREPTOMYCES alboniger. It inhibits protein synthesis by binding to RNA. It is an antineoplastic and antitrypanosomal agent and is used in research as an inhibitor of protein synthesis.
Peptide Biosynthesis
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
RNA, Transfer, Amino Acyl
Intermediates in protein biosynthesis. The compounds are formed from amino acids, ATP and transfer RNA, a reaction catalyzed by aminoacyl tRNA synthetase. They are key compounds in the genetic translation process.
Heptanoates
Salts and esters of the 7-carbon saturated monocarboxylic acid heptanoic acid.
Heinz Bodies
Abnormal intracellular inclusions, composed of denatured hemoglobin, found on the membrane of red blood cells. They are seen in thalassemias, enzymopathies, hemoglobinopathies, and after splenectomy.
Carcinoma, Krebs 2
Bloodletting
Puncture of a vein to draw blood for therapeutic purposes. Bloodletting therapy has been used in Talmudic and Indian medicine since the medieval time, and was still practiced widely in the 18th and 19th centuries. Its modern counterpart is PHLEBOTOMY.
Hemoglobins, Abnormal
Hemoglobins characterized by structural alterations within the molecule. The alteration can be either absence, addition or substitution of one or more amino acids in the globin part of the molecule at selected positions in the polypeptide chains.
Hematocrit
The volume of packed RED BLOOD CELLS in a blood specimen. The volume is measured by centrifugation in a tube with graduated markings, or with automated blood cell counters. It is an indicator of erythrocyte status in disease. For example, ANEMIA shows a low value; POLYCYTHEMIA, a high value.
Lipoxygenase
An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.
Arachidonate 15-Lipoxygenase
An enzyme that catalyzes the oxidation of arachidonic acid to yield 15-hydroperoxyarachidonate (15-HPETE) which is rapidly converted to 15-hydroxy-5,8,11,13-eicosatetraenoate (15-HETE). The 15-hydroperoxides are preferentially formed in NEUTROPHILS and LYMPHOCYTES.
Methionine
A sulfur-containing essential L-amino acid that is important in many body functions.
Anemia, Hypochromic
Anemia characterized by a decrease in the ratio of the weight of hemoglobin to the volume of the erythrocyte, i.e., the mean corpuscular hemoglobin concentration is less than normal. The individual cells contain less hemoglobin than they could have under optimal conditions. Hypochromic anemia may be caused by iron deficiency from a low iron intake, diminished iron absorption, or excessive iron loss. It can also be caused by infections or other diseases, therapeutic drugs, lead poisoning, and other conditions. (Stedman, 25th ed; from Miale, Laboratory Medicine: Hematology, 6th ed, p393)
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Peptide Chain Elongation, Translational
A process of GENETIC TRANSLATION, when an amino acid is transferred from its cognate TRANSFER RNA to the lengthening chain of PEPTIDES.
Erythrocytes, Abnormal
Oxygen-carrying RED BLOOD CELLS in mammalian blood that are abnormal in structure or function.
Poly A
A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
Micronucleus Tests
Induction and quantitative measurement of chromosomal damage leading to the formation of micronuclei (MICRONUCLEI, CHROMOSOME-DEFECTIVE) in cells which have been exposed to genotoxic agents or IONIZING RADIATION.
eIF-2 Kinase
A dsRNA-activated cAMP-independent protein serine/threonine kinase that is induced by interferon. In the presence of dsRNA and ATP, the kinase autophosphorylates on several serine and threonine residues. The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis.
RNA, Transfer
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Centrifugation, Density Gradient
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Erythrocyte Indices
ERYTHROCYTE size and HEMOGLOBIN content or concentration, usually derived from ERYTHROCYTE COUNT; BLOOD hemoglobin concentration; and HEMATOCRIT. The indices include the mean corpuscular volume (MCV), the mean corpuscular hemoglobin (MCH), and the mean corpuscular hemoglobin concentration (MCHC).
Spherocytosis, Hereditary
A group of familial congenital hemolytic anemias characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. The erythrocytes have increased osmotic fragility and are abnormally permeable to sodium ions.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Aurintricarboxylic Acid
A dye which inhibits protein biosynthesis at the initial stages. The ammonium salt (aluminon) is a reagent for the colorimetric estimation of aluminum in water, foods, and tissues.
RNA, Transfer, Met
A transfer RNA which is specific for carrying methionine to sites on the ribosomes. During initiation of protein synthesis, tRNA(f)Met in prokaryotic cells and tRNA(i)Met in eukaryotic cells binds to the start codon (CODON, INITIATOR).
Peptide Hydrolases
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Erythroid Precursor Cells
The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors.
Triticum
A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.
Anemia, Macrocytic
Anemia characterized by larger than normal erythrocytes, increased mean corpuscular volume (MCV) and increased mean corpuscular hemoglobin (MCH).
RNA Caps
Nucleic acid structures found on the 5' end of eukaryotic cellular and viral messenger RNA and some heterogeneous nuclear RNAs. These structures, which are positively charged, protect the above specified RNAs at their termini against attack by phosphatases and other nucleases and promote mRNA function at the level of initiation of translation. Analogs of the RNA caps (RNA CAP ANALOGS), which lack the positive charge, inhibit the initiation of protein synthesis.
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Guanosine Triphosphate
Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.
Anemia, Hemolytic, Autoimmune
Acquired hemolytic anemia due to the presence of AUTOANTIBODIES which agglutinate or lyse the patient's own RED BLOOD CELLS.
Protein Kinases
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
Iron Isotopes
Stable iron atoms that have the same atomic number as the element iron, but differ in atomic weight. Fe-54, 57, and 58 are stable iron isotopes.
Adenosine Triphosphate
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Hematologic Tests
Tests used in the analysis of the hemic system.
Peptide Elongation Factors
Protein factors uniquely required during the elongation phase of protein synthesis.
Iron Radioisotopes
Unstable isotopes of iron that decay or disintegrate emitting radiation. Fe atoms with atomic weights 52, 53, 55, and 59-61 are radioactive iron isotopes.
Hemoglobin, Sickle
An abnormal hemoglobin resulting from the substitution of valine for glutamic acid at position 6 of the beta chain of the globin moiety. The heterozygous state results in sickle cell trait, the homozygous in sickle cell anemia.
Macromolecular Substances
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Chromatography, DEAE-Cellulose
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Recombinant Proteins
Proteins prepared by recombinant DNA technology.
Anemia, Neonatal
The mildest form of erythroblastosis fetalis in which anemia is the chief manifestation.
Hemolysis
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
Encephalomyocarditis virus
The type species of CARDIOVIRUS causing encephalomyelitis and myocarditis in rodents, pigs, and monkeys. Infection in man has been reported with CNS involvement but without myocarditis.
Anemia, Hemolytic, Congenital
Hemolytic anemia due to various intrinsic defects of the erythrocyte.
Erythrocyte Volume
Volume of circulating ERYTHROCYTES . It is usually measured by RADIOISOTOPE DILUTION TECHNIQUE.
Edeine
Basic peptide antibiotic from Bacillus brevis. It exhibits broad-spectrum antibacterial activity and inhibits bacterial DNA synthesis.

Studies on a nonpolysomal ribonucleoprotein coding for myosin heavy chains from chick embryonic muscles. (1/2686)

A messenger ribonucleoprotein (mRNP) particle containing the mRNA coding for the myosin heavy chain (MHC mRNA) has been isolated from the postpolysomal fraction of homogenates of 14-day-old chick embryonic muscles. The mRNP sediments in sucrose gradient as 120 S and has a characteristic buoyant density of 1.415 g/cm3, which corresponds to an RNA:protein ratio of 1:3.8. The RNA isolated from the 120 S particle behaved like authentic MHC mRNA purified from chick embryonic muscles with respect to electrophoretic mobility and ability to program the synthesis of myosin heavy chain in a rabbit reticulocyte lysate system as judged by multi-step co-purification of the in vitro products with chick embryonic leg muscle myosin added as carrier. The RNA obtained from the 120 S particle was as effective as purified MHC mRNA in stimulating the synthesis of the complete myosin heavy chains in rabbit reticulocyte lysate under conditions where non-muscle mRNAs had no such effect. Analysis of the protein moieties of the 120 S particle by sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows the presence of seven distinct polypeptides with apparent molecular weights of 44,000, 49,000, 53,000, 81,000, 83,000, and 98,000, whereas typical ribosomal proteins are absent. These results indicate that the 120 S particles are distinct cellular entities unrelated to ribosomes or initiation complexes. The presence of muscle-specific mRNAs as cytoplasmic mRNPs suggests that these particles may be involved in translational control during myogenesis in embryonic muscles.  (+info)

Purification and characterization of initiation factor IF-E2 from rabbit reticulocytes. (2/2686)

Initiation factor IF-E2 was isolated from rabbit reticulocytes and purified 120-fold to near homogeneity by ammonium sulfate fractionation, column chromatography on DEAE-cellulose and phosphocellulose, and, when suitable, by sucrose density gradient centrifugation. The factor is a complex protein containing three nonidentical polypeptides of molecular weight 57,000, 52,000, and 36,000. It behaves as a complex throughout its purification and during polyacrylamide gel electrophoresis in nondenaturing buffer but its thress components are readily separated by electrophoresis in denaturing buffers. None of its components corresponds to any of the polypeptides of the other initiation factors or to any proteins of ribosomes washed in buffers containing a high salf concentration. A stoichiometric ratio of 1:1:1 was determined for the three polypeptides; based on the assumption of one copy each per complex, the calculated factor molecular weight is 145,000, a value in agreement with the measured value of 160,000. Initiation factor IF-E2 was radioactively labeled in vitro by reductive alkylation or by phosphorylation with a protein kinase also isolated from rabbit reticulocytes. Neither procedure causes a measurable change in the ability of the factor to form a ternary complex with GTP and the initiator methionyl-tRNA. 5'-Guanylyl-methylenediphosphonate may substitute for GTP, but only at relatively high concentrations. The binding of labeled initiation factor IF-E2 and methionyl-tRNA to the 40 S ribosomal subunit was studied by sucrose density gradient centrifugation. Appreciable binding of the factor is seen only when all three components of the ternary complex are included in the reaction mixture. The binding of either the factor or methionyl-tRNA was not stimulated by the addition of globin messenger RNA and initiation factor IF-E3. It was shown that all three polypeptide components of initiation factor IF-E2 are bound to these nascent initiation complexes.  (+info)

BE-31405, a new antifungal antibiotic produced by Penicillium minioluteum. I. Description of producing organism, fermentation, isolation, physico-chemical and biological properties. (3/2686)

A new antifungal antibiotic, BE-31405, was isolated from the culture broth of a fungal strain, Penicillium minioluteum F31405. BE-31405 was isolated by adsorption on high porous polymer resin (Diaion HP-20), followed by solvent extraction, precipitation and crystallization. BE-31405 showed potent growth inhibitory activity against pathogenic fungal strains such as Candida albicans, Candida glabrata and Cryptococcus neoformans, but did not show cytotoxic activity against mammalian cells such as P388 mouse leukemia. The mechanism studies indicated that BE-31405 inhibited the protein synthesis of C. albicans but not of mammalian cells.  (+info)

unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. (4/2686)

Initiation of translation of the animal picornavirus RNAs occurs via a mechanism of direct ribosome entry, which requires a segment of the 5' UTR of the RNA, known as the internal ribosome entry site (IRES). In addition, translation of the enterovirus and rhinovirus (HRV) subgroups requires cellular trans-acting factors that are absent from, or limiting in rabbit reticulocytes, but are more abundant in HeLa cell extracts. It has been shown previously that HeLa cells contain two separable activities, each of which independently stimulates HRV IRES-dependent translation when used to supplement reticulocyte lysate; one of these activities was identified as polypyrimidine tract-binding protein (PTB). Here, the purification of the second activity is achieved by use of an RNA-affinity column based on the HRV 5' UTR. It comprises two components: a 38-kD protein (p38), which is a novel member of the GH-WD repeat protein family and has no intrinsic RNA-binding activity; and a 96- to 97-kD protein doublet, which was identified as unr, an RNA-binding protein with five cold-shock domains. Coimmunoprecipitation with antibodies against either protein shows that the two proteins interact with each other, and thus p38 is named unrip (unr-interacting protein). Recombinant unr acts synergistically with recombinant PTB to stimulate translation dependent on the rhinovirus IRES. In contrast, unr did not significantly augment the PTB-dependent stimulation of poliovirus IRES activity.  (+info)

Cell-free immunology: construction and in vitro expression of a PCR-based library encoding a single-chain antibody repertoire. (5/2686)

A novel cloning-independent strategy has been developed to generate a combinatorial library of PCR fragments encoding a murine single-chain antibody repertoire and express it directly in a cell-free system. The new approach provides an effective alternative to the techniques involving in vivo procedures of preparation and handling large libraries of antibodies. The possible use of the described strategy in the ribosome display is discussed.  (+info)

Gbeta5 prevents the RGS7-Galphao interaction through binding to a distinct Ggamma-like domain found in RGS7 and other RGS proteins. (6/2686)

The G protein beta subunit Gbeta5 deviates significantly from the other four members of Gbeta-subunit family in amino acid sequence and subcellular localization. To detect the protein targets of Gbeta5 in vivo, we have isolated a native Gbeta5 protein complex from the retinal cytosolic fraction and identified the protein tightly associated with Gbeta5 as the regulator of G protein signaling (RGS) protein, RGS7. Here we show that complexes of Gbeta5 with RGS proteins can be formed in vitro from the recombinant proteins. The reconstituted Gbeta5-RGS dimers are similar to the native retinal complex in their behavior on gel-filtration and cation-exchange chromatographies and can be immunoprecipitated with either anti-Gbeta5 or anti-RGS7 antibodies. The specific Gbeta5-RGS7 interaction is determined by a distinct domain in RGS that has a striking homology to Ggamma subunits. Deletion of this domain prevents the RGS7-Gbeta5 binding, although the interaction with Galpha is retained. Substitution of the Ggamma-like domain of RGS7 with a portion of Ggamma1 changes its binding specificity from Gbeta5 to Gbeta1. The interaction of Gbeta5 with RGS7 blocked the binding of RGS7 to the Galpha subunit Galphao, indicating that Gbeta5 is a specific RGS inhibitor.  (+info)

Human molybdopterin synthase gene: identification of a bicistronic transcript with overlapping reading frames. (7/2686)

A universal molybdenum-containing cofactor (MoCo) is essential for the activity of all human molybdoenzymes, including sulphite oxidase. The free cofactor is highly unstable, and all organisms share a similar biosynthetic pathway. The involved enzymes exhibit homologies, even between bacteria and humans. We have exploited these homologies to isolate a cDNA for the heterodimeric molybdopterin (MPT)-synthase. This enzyme is necessary for the conversion of an unstable precursor into molybdopterin, the organic moiety of MoCo. The corresponding transcript shows a bicistronic structure, encoding the small and large subunits of the MPT-synthase in two different open reading frames (ORFs) that overlap by 77 nucleotides. In various human tissues, only one size of mRNA coinciding with the bicistronic transcript was detected. In vitro translation and mutagenesis experiments demonstrated that each ORF is translated independently, leading to the synthesis of a 10-kDa protein and a 21-kDa protein for the small and large subunits, respectively, and indicated that the 3'-proximal ORF of the bicistronic transcript is translated by leaky scanning.  (+info)

Evidence for and consequences of chronic heme deficiency in Belgrade rat reticulocytes. (8/2686)

The Belgrade rat has a microcytic, hypochromic anemia inherited as an autosomal recessive trait (gene symbol b). Transferrin-dependent iron uptake is defective because of a mutation in Nramp2 (now DMT1, also called DCT1), the protein responsible for endosomal iron efflux. Hence, Belgrade reticulocytes are iron deficient. We show that a chromatographic method is able to measure the amount of 'free' heme in reticulocytes. Most of the 'free' heme is the result of biosynthesis. Succinylacetone, an inhibitor of heme synthesis, decreases the level of 'free' heme and cycloheximide, an inhibitor of globin synthesis, increases the 'free' heme level. In a pulse-chase experiment with 59Fe-transferrin, the 'free' heme pool behaves as an intermediate, with a half-life of just over 2 h. Belgrade reticulocytes contain about 40% as much 'free' heme as do heterozygous or homozygous reticulocytes. This deficiency of 'free' heme slows initiation of translation in Belgrade reticulocytes by increasing the level of an inhibitor of initiation. Thus the Belgrade rat makes a whole animal model available with chronic heme deficiency.  (+info)

Degradation of RNA in rat reticulocytes. I. Purification and properties of rat reticulocyte RNase<...
TY - JOUR. T1 - Degradation of RNA in rat reticulocytes. I. Purification and properties of rat reticulocyte RNase. AU - Goto, Sataro. AU - Mizuno, DenIchi. PY - 1971/7. Y1 - 1971/7. N2 - A RNase has been partially purified from rat reticulocytes induced by phenylhydrazine. This enzyme has an optimum pH of 7.5 and degrades RNA endonucleolytically as evidenced by the analysis of degradation products. The activity is destroyed by heat treatment (pH 6.5, 80 °, 5 min). Many metal ions are inhibitory for the activity. The enzyme was inactivated almost completely by 0.5 mm HgCl2. Monovalent ions, including Nad, KCl, NH4Cl, and (NH4)2SO4, inhibit the enzyme by about 90% at concentrations of 0.1-0.2 m. The molecular weight of this enzyme is about 16,000 as determined by gel filtration. A latent RNase with higher molecular weight is present in the crude extract of the cells.. AB - A RNase has been partially purified from rat reticulocytes induced by phenylhydrazine. This enzyme has an optimum pH of 7.5 ...
https://hungary.pure.elsevier.com/en/publications/degradation-of-rna-in-rat-reticulocytes-i-purification-and-proper
Flexi® Rabbit Reticulocyte Lysate SystemFlexi® Rabbit Reticulocyte Lysate System
Flexi® Rabbit Reticulocyte Lysate System allows optimization of translation reactions for a range of parameters, including Mg(2+) and K(+) and DTT concentration.
https://www.promega.com/products/protein-expression/cell-free-protein-expression/flexi-rabbit-reticulocyte-lysate-system/
Reticulocyte - WikipediaReticulocyte - Wikipedia
Reticulocytes are immature red blood cells, typically composing about 1% of the red blood cells in the human body. In the process of erythropoiesis (red blood cell formation), reticulocytes develop and mature in the bone marrow and then circulate for about a day in the blood stream before developing into mature red blood cells. Like mature red blood cells, in mammals reticulocytes do not have a cell nucleus. They are called reticulocytes because of a reticular (mesh-like) network of ribosomal RNA that becomes visible under a microscope with certain stains such as new methylene blue and Romanowsky stain. To accurately measure reticulocyte counts, automated counters use a combination of laser excitation, detectors and a fluorescent dye that marks RNA and DNA (such as titan yellow or polymethine). Reticulocytes can be distinguished from other circulating cells because they emit a signal that is neither strong (like lymphocytes) nor weak (like red blood cells). Reticulocytes appear slightly bluer ...
https://en.wikipedia.org/wiki/Reticulocyte
reticulocytes: Definition, Word Game Analysisreticulocytes: Definition, Word Game Analysis
reticulocytes Definition, reticulocytes Best Plays of reticulocytes in Scrabble® and Words With Friends, Length tables of words in reticulocytes, Word growth of reticulocytes, Sequences of reticulocytes
https://www.litscape.com/word_analysis/reticulocytes
The Structure and Composition of Rat Reticulocytes. II. Phospholipid and Total Cholesterol in Reticulocytes | Blood | American...The Structure and Composition of Rat Reticulocytes. II. Phospholipid and Total Cholesterol in Reticulocytes | Blood | American...
Abstract. 1. The rat reticulocytes examined contain 6.7 ± 0.2 mg. of phospholipid per ml. of packed cells, 49 per cent more than rat erythrocytes.2. Reticulocy
https://ashpublications.org/blood/article/20/5/557/37671/The-Structure-and-Composition-of-Rat-Reticulocytes
PIEZO1 gain-of-function mutations delay reticulocyte maturation in hereditary xerocytosis<...PIEZO1 gain-of-function mutations delay reticulocyte maturation in hereditary xerocytosis<...
TY - JOUR. T1 - PIEZO1 gain-of-function mutations delay reticulocyte maturation in hereditary xerocytosis. AU - Moura, Pedro Luís. AU - Hawley, Bethan R. AU - Dobbe, Johannes G. G.. AU - Streekstra, Geert J.. AU - Rab, Minke A.E. AU - Bianchi, Paola. AU - van Wijk, Richard AU - Toye, Ash M. AU - Satchwell, Timothy. PY - 2019/10/17. Y1 - 2019/10/17. U2 - 10.3324/haematol.2019.231159. DO - 10.3324/haematol.2019.231159. M3 - Article (Academic Journal). C2 - 31624108. JO - Haematologica. JF - Haematologica. SN - 0390-6078. ER - ...
https://research-information.bris.ac.uk/en/publications/piezo1-gain-of-function-mutations-delay-reticulocyte-maturation-i
Scientists Debate--Parisottos Response to FerrariScientists Debate--Parisottos Response to Ferrari
2)Why were reticulocyte levels decreased when Hemoglobin was increased in the two tests allegedly collected/performed at altitude prior to the 2009 tour. This scenario is consistent with previous administration of stimulating agents such as EPO and or previous blood re-infusion. In the original post …. from October 16, 2008 the average reticulocyte levels are 1.1 but from May 18, 2009 the average reticulocyte levels are 0.6. This change/reduction in reticulocyte production during competition is not due to hemodilution however as percentage values for reticulocytes are not affected by this physiological response. This reduction reflects a blunted response to normal red blood cell production and can be achieved by micro-doping over an extended period of time or blood doping over shorter periods. ...
http://downthebackstretch.blogspot.com/2012/09/scientists-debate-parisottos-response.html
Patente US5858789 - Method for making a reticulocyte assay control - Google PatentesPatente US5858789 - Method for making a reticulocyte assay control - Google Patentes
A pre-determined concentration of stabilized, maturation-arrested porcine reticulocytes in a red blood cell base, useful as a reticulocyte control composition. The composition can be provided in the form of a concentrated reticulocyte composition, to be diluted to a desired final reticulocyte concentration at the time of use. The composition can also be provided in the form of a diluted, ready-to-use control composition. Also included is a method of preparing such a composition, the method involving sequential steps of forming and sedimenting Rouleaux bodies.
http://www.google.es/patents/US5858789
北京大学医学部机构知识库([email protected]): Study of the microrheological characteristics of reticulocytes with Co-60 radiation
The microrheological characteristics of reticulocytes in vivo was studied. Method of anemia in rabbits induced by Co-60 radiation was used. The measurements of percent of reticulocytes, deformation index, orientation index, etc in the processes of reticulocytes changing into erythrocytes in vivo for 72 It were performed. It was shown that there were obvious changes in the reticulocytes rheological characteristics in the courses of this changing. Therefore, the found had some basic theoretical and clinical significance. ...
http://ir.bjmu.edu.cn/handle/400002259/51191
Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation - MORU Tropical...Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation - MORU Tropical...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
https://www.tropmedres.ac/publications/745850
Hsp90 Controls GSK-3β Kinase Specificity | Science SignalingHsp90 Controls GSK-3β Kinase Specificity | Science Signaling
Glycogen synthase kinase 3 (GSK3) has long been known as a signaling component in insulin regulation of metabolism and, more recently, as a key part of the Wnt signaling pathway regulating cell proliferation, cell fate, and other processes during development. Unlike most other kinases, GSK3 is constitutively active and is regulated by inhibition. Full expression of this constitutive activity in the mammalian enzyme appears to require phosphorylation of a tyrosine residue in the activation loop of GSK3, which the enzyme accomplishes by intramolecular autophosphorylation, even though the kinase phosphorylates strictly serine and threonine residues on its exogenous substrates. Lochhead et al. explored the mechanism by which this switch in residue specificity is possible. Tagged GSK-3β synthesized in a rabbit reticulocyte lysate translation system showed rapid autophosphorylation that was inhibited by inhibitors of the molecular chaperone protein Hsp90. This chaperone-assisted tyrosine kinase ...
https://stke.sciencemag.org/content/2006/362/tw395
Reticulocyte Count Blood Test
Reticulocytes are immature red blood cells and are measured to evaluate the function of your bone marrow. Normally as it matures, the red blood cell loses its nucleus. The remaining parts of the cell produce a characteristic color making them. ...
http://reverse-aging.info/reticulocyte-count-blood-test/
Pv GAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis | Parasites &...Pv GAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis | Parasites &...
Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and
https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-2183-8/figures/2
Liquichek Reticulocyte Control (A)Liquichek Reticulocyte Control (A)
Liquichek Reticulocyte Control (A),NEW Liquichek Reticulocyte Control (A)is an assayed whole blood control for evaluating the precision of automated methods of reticulocyte counting.,medicine,medical supply,medical supplies,medical product
http://bio-medicine.org/medicine-products/Liquichek-Reticulocyte-Control--28A-29-18257-1/
Reticulocytes in the blood - the optimal amunt of Reticulocytes in the bloodReticulocytes in the blood - the optimal amunt of Reticulocytes in the blood
Reticulocytes in the blood is important for undisturbed performance of all body functions. Check the level of Reticulocytes in the body and avoid disease.
http://www.lab-test-results.com/en/157
Reticulocyte Index CalculatorReticulocyte Index Calculator
This reticulocyte index calculator helps you calculate the reticulocyte production index RPI based on absolute count, hematocrit or hemoglobin in two tabs.
https://www.thecalculator.co/health/Reticulocyte-Index-Calculator-692.html
Universality and Structure of the N-end Rule - CaltechAUTHORSUniversality and Structure of the N-end Rule - CaltechAUTHORS
Our previous work has shown that, in the yeast Saccharomyces cerevisiae, any of the eight stabilizing amino-terminal residues confers a long (, 20 h) half-life on a test protein β-galactosidase (β gal), whereas 12 destabilizing amino-terminal residues confer on β gal half-lives from less than 3 min to 30 min. We now show that an analogous single-residue code (the N-end rule) operates in an in vitro system derived from mammalian reticulocytes. We also show that the N-end rule has a hierarchical structure. Specifically, amino-terminal Glu and Asp (and also Cys in reticulocytes) are secondary destabilizing residues in that they are destabilizing through their ability to be conjugated to primary destabilizing residues such as Arg. Amino-terminal Gln and Asn are tertiary destabilizing residues in that they are destabilizing through their ability to be converted, via selective deamidation, into secondary destabilizing residues Glu and Asp. Furthermore, in reticulocytes, distinct types of the ...
https://authors.library.caltech.edu/107818/
Plus itPlus it
The ability to transcribe and translate proteins in vitro is a valuable tool for probing protein structure and function, and the role of specific amino acid residues in protein activity. Large amounts of the protein of interest, with or without targeted mutations, can be produced much more quickly and can be radiolabeled to a much higher specific activity than in whole cells. Furthermore, the rabbit reticulocyte lysate system, as used for these studies, provides many of the eucaryotic post-translational modification activities, cofactors, hsp90, and other heat-shock proteins that are lacking in bacterial and yeast systems. Indeed, we found that when murine AhR and Arnt were separately translated in reticulocyte lysate and mixed together, TCDD binding and DRE binding of the resultant complex were very similar to that formed in mouse hepatoma cell cytosol. Coimmunoprecipitation of unliganded [35S]AhR with α-hsp90 antibody further indicates its comparability to the cytosolic AhR complex ...
http://molpharm.aspetjournals.org/content/63/2/392
glossary:reticulocytes
A young red blood cell that usually remains in the bone marrow with only a few venturing out into the circulating blood. The number of reticulocytes in blood rises when red cell production is unusually vigorous, as after a major bleeding episode or after the bone marrow has been suppressed and is regenerating. The reticulocyte is so named because it has a fine reticulum representing ribosomal remains. ...
http://m.www.lymphedemapeople.com/wiki/doku.php?id=glossary:reticulocytes
Learn MoreLearn More
Martha Sola-Visner, MD, Associate Professor of Pediatrics, Boston Childrens Hospital and Harvard University, Boston, MA. This session will review the incidence, clinical significance, and diagnostic evaluation of anemia, iron deficiency and thrombocytopenia in neonates. I will also discuss the clinical utility of current tools and parameters to assist in the evaluation of anemia, iron deficiency and thrombocytopenia in the neonatal population, including recent studies on Reticulocyte Hemoglobin (RET-He) and the Immature Platelet Fraction (IPF). Objectives:. ...
https://www.sysmex.com/us/en/Education/Webinars/Pages/LearnMore.aspx?WebinarID=89&Upcoming=0
PolychromatophiliaPolychromatophilia
Polychromatophilia refers to how blood cells look under a microscope when the cells are stained with special dyes. It means there is more staining than normal with certain dyes. The extra staining is due to too many immature red blood cells (RBCs) called reticulocytes. These cells have a blue-colored center. Increased reticulocytes are the...
https://www.baptistjax.com/health-library/disease/polychromatophilia
invitro translationinvitro translation
I am going to try invitro translation. Can anybody tell me where I ,can get the good kit with reasonable price? I prefer non-radioactive ,system. Thanks in advance. , ,Hua , Try Promega: Rabbit Reticulocyte Lysate Systems. Works very good, but is a little expensive if you only by one kit. Anyways, try to negotiate a price. Andre ...
http://www.bio.net/bionet/mm/methods/1998-January/063809.html
CD71 antibodies, human - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - España
Clone REA902 recognizes the human CD71 antigen, a transferrin receptor, which is essential for iron transport into proliferating cells. CD71 is expressed on marrow stromal cells (MSCs) from bone marrow, on activated T and B lymphocytes, macrophages, and all proliferating cells. It is upregulated on lymphocytes during proliferative responses to antigens or mitogens but is not expressed on resting lymphocytes. CD71 is present on reticulocytes and erythroid progenitors in fetal liver, cord blood, and peripheral blood, yet it is lost as these differentiate to mature erythrocytes. Additional information: Clone REA902 displays negligible binding to Fc receptors. - España
https://www.miltenyibiotec.com/ES-en/products/macs-flow-cytometry/antibodies/primary-antibodies/cd71-antibodies-human-rea902-1-50.html
CD71 Antibody, anti-human, REAfinity™ | Recombinant antibodies | MACS Antibodies | Products | Miltenyi Biotec | Principat d...
Clone REA902 recognizes the human CD71 antigen, a transferrin receptor, which is essential for iron transport into proliferating cells. CD71 is expressed on marrow stromal cells (MSCs) from bone marrow, on activated T and B lymphocytes, macrophages, and all proliferating cells. It is upregulated on lymphocytes during proliferative responses to antigens or mitogens but is not expressed on resting lymphocytes. CD71 is present on reticulocytes and erythroid progenitors in fetal liver, cord blood, and peripheral blood, yet it is lost as these differentiate to mature erythrocytes.Additional information: Clone REA902 displays negligible binding to Fc receptors. | Principat dAndorra
https://www.miltenyibiotec.com/AD-en/products/cd71-antibody-anti-human-reafinity-rea902.html
Haemoglobin synthesis in reticulocytes of prenatal f/f anaemic mice. by R J. Cole, T Regan et al.Haemoglobin synthesis in reticulocytes of prenatal f/f anaemic mice. by R J. Cole, T Regan et al.
Cole, R J.; Regan, T; and Tarbutt, R G., Haemoglobin synthesis in reticulocytes of prenatal f/f anaemic mice. (1972). Subject Strain Bibliography 1972. 2819 ...
https://mouseion.jax.org/ssbb1972/2819/
网织红细胞 (reticulocytes): Topics by WorldWideScience.org网织红细胞 (reticulocytes): Topics by WorldWideScience.org
In Italian and Chinese patients with the α-thalassemia syndromes the production of α-chain of normal hemoglobin is decreased relative to that of β-chain in reticulocytes. In this study the relative rates of α- and β-chain synthesis were determined in members of three Negro families with α-thalassemi... DRIVER (Chinese) ...
https://worldwidescience.org/topicpages/multi/ZH/r/reticulocytes.html
Reticulocyte Procedure With Miller Eye Disc - BoneReticulocyte Procedure With Miller Eye Disc - Bone
A Miller Eye Disc is placed inside the microscope eyepiece as an aid to counting reticulocytes. This reticule is a large square inside a small square and
https://www.guwsmedical.info/bone-marrow/reticulocyte-procedure-with-miller-eye-disc.html
Nursing Central | reticulocyteNursing Central | reticulocyte
reticulocyte was found in Nursing Central. Nursing Central is the award-winning, complete mobile solution for nurses and students. Look up information on diseases, tests, and procedures; then consult the database with 5,000+ drugs or refer to 65,000+ dictionary terms.
https://nursing.unboundmedicine.com/nursingcentral/search?catcode=539&st=OSS&search=reticulocyte
Retics and DNA binding proteinsRetics and DNA binding proteins
Does anyone use DNA binding proteins expressed in rabbit reticulocyte lysates to do gel shift assays? This system would allow easy and quick expression of a suspected DNA binding protein that I could study (much easier than trying to express and purify the protein). I would specifically like to know if the other proteins in the system or maybe even the DNA added would somehow interfere in a gel shift assay (although I guess its no different than using cell/nuclear extracts). If anyone has experience with this or know of a reference could you please let me know? Thanks. -- Steve Some day I will get the hell out of Wisconsin Rodems Then I am here for the Lee family renioun ... shur-wajo-shur ...
http://www.bio.net/bionet/mm/methods/1994-April/013582.html
Bands to the Left, Reticulocytes to the Right: Im Stuck in the Middle Reading the DifferentialBands to the Left, Reticulocytes to the Right: Im Stuck in the Middle Reading the Differential
Analyze the implications of red blood cell indices in the determination of the various anemias, and interpret the response of the white blood cell differential in viruses and bacterial infections. Contact hours available until 10/10/18. Requirements for Successful Completion: Complete the...
https://library.annanurse.org/anna/sessions/3313/view
Reticulocytes - Loyola University Health SystemReticulocytes - Loyola University Health System
The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. © 1997- A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited ...
http://loyolauniversity.adam.com/content.aspx?productId=101&pid=2&gid=1491
The Wie Factor « m a r k a n d e y aThe Wie Factor « m a r k a n d e y a
The Chosun Ilbo examines the Wie Factor: Her Korean-ness also plays a part. Despite her American nationality, when Wie answers questions asked in English in her not-so-fluent Korean, and when she introduces herself by her Korean name Sung-mi instead of Michelle, hearts here melt. Wie stressed that heritage during last weeks visit. In contrast to…
https://markandeya.wordpress.com/2006/05/08/the-wie-factor/
CSHL Library
Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA ...
http://libfe.cshl.edu/cshlpublications/holdings.php?type=authorf&criteria=Philipson,%20L.&boolean=FALSE
Day +162: Stability. | The Bald Heme-iolaDay +162: Stability. | The Bald Heme-iola
Day +162. My counts are still stable with the HgB at 11.5 g/dL, WBCs at 4.5 x10e9/L, ANC at 2.18 x10e9/L, platelets at 143 x10e9/L, the latter three all in the normal range. My body still doesnt seem to like making RBCs, but its doing the best it can with elevated reticulocytes (RBC pre-cursors) at…
https://thebaldhemiola.com/2016/04/07/day-162-stability/comment-page-1/
Retic Count
This test measures the number of reticulocytes in your blood. It can be used to diagnose anemia and find out why you have a disease.
https://healthinfo.camc.org/RelatedItems/167,retic_ct
Pentra XLR - HORIBAPentra XLR - HORIBA
Up to 80 samples / hour Large capacity autoloader (100 tubes) 36 parameters CBC / 5 part DIFF / Full reticulocyte parameters Stat sampling on...
https://www.horiba.com/at/medical/products/hematology/abx-pentra-xl80-pentra-xlr/pentra-xlr-details/pentra-xlr-23127/
The effect of puromycin on rabbit reticulocyte ribosomes.<...
TY - JOUR. T1 - The effect of puromycin on rabbit reticulocyte ribosomes.. AU - Allen, David W. AU - ZAMECNIK, P. C.. PY - 1962/6/11. Y1 - 1962/6/11. UR - http://www.scopus.com/inward/record.url?scp=50549177969&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=50549177969&partnerID=8YFLogxK. M3 - Article. VL - 55. SP - 865. EP - 874. JO - Biochimica et Biophysica Acta - General Subjects. JF - Biochimica et Biophysica Acta - General Subjects. SN - 0304-4165. ER - ...
https://minnesota-staging.pure.elsevier.com/en/publications/the-effect-of-puromycin-on-rabbit-reticulocyte-ribosomes-2
Reticulocyte count financial definition of Reticulocyte countReticulocyte count financial definition of Reticulocyte count
Definition of Reticulocyte count in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is Reticulocyte count? Meaning of Reticulocyte count as a finance term. What does Reticulocyte count mean in finance?
https://financial-dictionary.thefreedictionary.com/Reticulocyte+count
Discrepancy between the percentage of hypochromic erythrocytes and the reticulocyte hemoglobin content in hemodialysis patients...Discrepancy between the percentage of hypochromic erythrocytes and the reticulocyte hemoglobin content in hemodialysis patients...
The percentage of hypochromic erythrocytes (%HYPO) and the reticulocyte hemoglobin content (CHr) have been used for the diagnosis of iron deficiency (ID). However, we found a discrepancy between %HYPO and CHr values in some hemodialysis patients. Hemodialysis patients receiving recombinant human ery …
https://pubmed.ncbi.nlm.nih.gov/16024336/?dopt=Abstract
Corrected reticulocyte percentage (CRP) | definition of corrected reticulocyte percentage (CRP) by Medical dictionaryCorrected reticulocyte percentage (CRP) | definition of corrected reticulocyte percentage (CRP) by Medical dictionary
Looking for online definition of corrected reticulocyte percentage (CRP) in the Medical Dictionary? corrected reticulocyte percentage (CRP) explanation free. What is corrected reticulocyte percentage (CRP)? Meaning of corrected reticulocyte percentage (CRP) medical term. What does corrected reticulocyte percentage (CRP) mean?
https://medical-dictionary.thefreedictionary.com/corrected+reticulocyte+percentage+
Reticulocyte CountReticulocyte Count
This page includes the following topics and synonyms: Reticulocyte Count, Retic Count, Absolute Reticulocyte Count, Reticulocyte Index, Reticulocytopenia, Reticulocytosis.
https://fpnotebook.com/legacy/HemeOnc/Lab/RtclcytCnt.htm
PLOS Pathogens: Host Reticulocytes Provide Metabolic Reservoirs That Can Be Exploited by Malaria ParasitesPLOS Pathogens: Host Reticulocytes Provide Metabolic Reservoirs That Can Be Exploited by Malaria Parasites
Author Summary Malaria, caused by the Apicomplexan parasites Plasmodium spp., is a deadly disease which poses a huge health and economic burden over many populations in the world, mostly in sub-Saharan Africa and Asia. To design new intervention strategies and to improve upon existing drugs against malaria, it is useful to understand the biochemistry of the Plasmodium parasite and its metabolic interplay with the host. Some species of Plasmodium such as P. vivax grow exclusively in reticulocytes (immature erythrocytes) whereas others e.g. P. falciparum will also readily multiply in mature erythrocytes. We asked the questions, do these two classes of host cell offer different resources for parasite survival and could these resources influence antimalarial drug efficacy? We used metabolomics to compare rodent reticulocytes and mature erythrocytes and identified that the metabolome of the former is more diverse and enriched. Gene disruption in the reticulocyte preferring rodent malaria parasite P. berghei
https://journals.plos.org/plospathogens/article/comments?id=10.1371/journal.ppat.1004882
Giemsa-stained wet mount based method for reticulocyte quantification: a viable alternative in resource limited or malaria...
The quantity of circulating reticulocytes is an important indicator of erythropoietic activity in response to a wide range of haematological pathologies. While most modern laboratories use flow cytometry to quantify reticulocytes, most field laboratories still rely on subvital staining. The specialist subvital stains, New Methylene Blue (NMB) and Brilliant Crésyl Blue are often difficult to procure, toxic, and show inconsistencies between batches. Here we demonstrate the utility of Giemsas stain (commonly used microbiology and parasitology) in a subvital manner to provide an accurate method to visualize and count reticulocytes in blood samples from normal and malaria-infected individuals.
https://www.ndm.ox.ac.uk/publications/394886
Blood Test: Reticulocyte CountBlood Test: Reticulocyte Count
This test measures the rate at which reticulocytes (immature red blood cells) are made in the bone marrow and enter the bloodstream. A reticulocyte count can provide information about a childs anemia.
http://kidshealth.org/Nemours/en/parents/reticulocyte.html?view=rr
An Adventure with Landon: March 2014An Adventure with Landon: March 2014
When I made it back to Kansas City that evening, Karen informed me that they had performed a reticulocyte count for Landon. Reticulocytes are the precursors to red blood cells. When your body doesnt have enough red blood cells, your bone marrow should respond by making more and the overall population of cells should be younger. Normal levels in an infant should be between 2 and 6 percent and that number should rise significantly in an anemic baby. Despite being very anemic, Landons reticulocyte count was .5%. We agreed to a blood transfusion and waited for test results as to why his count was so low. Doctors determined that his bone marrow was not producing red cells anymore. We arent sure if this is due to a neurologic dysfunction or inability of his gut to absorb nutrients but the end result is essentially the same. If his body does not respond, he will slowly become more and more anemic until he becomes weak and passes away. The reality of this is that transfused red blood cells only ...
http://anadventurewithlandon.blogspot.com/2014/03/
An Adventure with Landon: 2014An Adventure with Landon: 2014
When I made it back to Kansas City that evening, Karen informed me that they had performed a reticulocyte count for Landon. Reticulocytes are the precursors to red blood cells. When your body doesnt have enough red blood cells, your bone marrow should respond by making more and the overall population of cells should be younger. Normal levels in an infant should be between 2 and 6 percent and that number should rise significantly in an anemic baby. Despite being very anemic, Landons reticulocyte count was .5%. We agreed to a blood transfusion and waited for test results as to why his count was so low. Doctors determined that his bone marrow was not producing red cells anymore. We arent sure if this is due to a neurologic dysfunction or inability of his gut to absorb nutrients but the end result is essentially the same. If his body does not respond, he will slowly become more and more anemic until he becomes weak and passes away. The reality of this is that transfused red blood cells only ...
http://anadventurewithlandon.blogspot.com/2014/
Point Mutations in the WD40 Domain of Eed Block Its Interaction with Ezh2 | Molecular and Cellular BiologyPoint Mutations in the WD40 Domain of Eed Block Its Interaction with Ezh2 | Molecular and Cellular Biology
Fig. 4. Ezh2 binds Eed in vitro. Ezh2 or Eed mRNAs were translated in a rabbit reticulocyte cell-free system in the presence of [35S]methionine. The translational products were incubated (1 h at 4°C) with glutathione-agarose beads bearing either the wild-type GST-Eed or GST-Eed mutants I287N (T1031A) and L290P (T1040C) (A), GST-Ezh2 (aa 1 to 192) (C and D) or GST-K (D). After binding, the beads were washed and boiled in SDS buffer, and eluted proteins were analyzed by SDS-PAGE and autoradiography. The Coomassie blue-stained gel of the experiment in panel A, lanes 1 to 3, is shown in panel B. (C) Eed and T1040C translational products were bound to GST-Ezh2 beads. Lanes 5 and 6 display the Coomassie blue-stained gel corresponding to lanes 3 and 4. (D) Eed, EedΔN, and EedΔC translational products were mixed and bound to either GST-Ezh2 or GST-K beads. (E) Eed constructs used in the experiments. Open box, GST (A) or His/T7 tag (pET28 vector) (C and D); shaded box, N-terminal part of Eed; solid ...
https://mcb.asm.org/content/18/10/5634/figures-only
S AnandS Anand
The heme-regulated enkaryotic initiation factor-2α (eIF-2α) kinase, also called the heme-regulated inhibitor (HRI), is a key regulator of protein synthesis in mammalian reticulocyte. HRI is almost undetectable in blood samples of normal rabbits and it increases by 12-15-fold in the reticulocytes of anemic rabbits. In order to determine if such an increase in the quantity of HRI is gradual during anemia, and if it could be an indicator of anemia, we have carried out a detailed analysis on the expression of HRI and its eIF-2α kinase activity in rabbit reticulocyte lysates during various stages of acetylphenylhydrazine (APH)-induced anemia. In a 9-day schedule of induction of anemia, using an anti-HRI monoclonal antibody, HRI was detectable immediately after completion of fourth injection (day 5) and it increased gradually during the entire period reaching its maximum (24-fold) on day 9. Furthermore, when rabbits recovered from anemia due to individual response to the drug, quantity of HRI ...
https://www.ias.ac.in/listing/bibliography/jbsc/S._Anand
PERHIMPUNAN REUMATOLOGI INDONESIA::.PERHIMPUNAN REUMATOLOGI INDONESIA::.
IJR Vol 9 No.2, July - December 2017; Overview of Anemia among Systemic Lupus Erythematosus Patients in Reproductive Age Women based on Reticulocyte Hemoglobin Equivalent (RET-He) Level and Reticulocyte ...
http://reumatologi.or.id/reuarttail?id=162
Errata Corrige | HaematologicaErrata Corrige | Haematologica
Figure 3. CD47 and CD147 expression on red blood cells (RBC) and on reticulocytes. For each group under study (Control, hydroxyurea-treated [HU], VOC and Non-VOC), the number of receptors/RBC (A, C) or reticulocytes (B, D) are indicated. Values are given as median, lower quartile, upper quartile, minimum and maximum for each group of patients and controls. ...
http://www.haematologica.org/content/94/4/598
The isolation and characterisation of specific RNA molecules and RNA-DNA complexes in animal cells - Enlighten: ThesesThe isolation and characterisation of specific RNA molecules and RNA-DNA complexes in animal cells - Enlighten: Theses
1. The identification, isolation and characterisation of a mammalian mRNA required choice of a cell type which synthesises only one or a few proteins. The mammalian reticulocyte synthesises globin almost exclusively. This cell type can be isolated in large quantities and has low endogenous ribonuclease activity, thus making it a suitable system for the isolation of large amounts of undegraded RNA. Preliminary experiments indicated that the reticulocyte RNA component sedimenting at 9s had many of the characteristics expected of the globin mRNAs. 2. The characterisation of an mRNA which can only be labelled to a small extent in vivo and which is only about l of the total RNA requires the development of large-scale isolation procedures. Preliminary experiments designed to circumvent the difficulty of labelling reticulocyte 9s RNA in vivo involved the isolation of uridine-9s RNA from cultures of 14 day mouse embryo liver cells. The technique of preparative polyacrylamide gel electrophoresis was ...
http://theses.gla.ac.uk/73874/
Reticulocyte count | UF Health, University of Florida HealthReticulocyte count | UF Health, University of Florida Health
DefinitionReticulocytes are slightly immature red blood cells. A reticulocyte count is a blood test that measures the amount of these cells in the blood.Alternative NamesAnemia - reticulocyteHow the Test is PerformedA blood sample is needed.
https://ufhealth.org/reticulocyte-count
Reticulocyte Count Low to Normal Causes & Reasons - SymptomaReticulocyte Count Low to Normal Causes & Reasons - Symptoma
Reticulocyte Count Low to Normal Symptom Checker: Possible causes include Anemia. Check the full list of possible causes and conditions now! Talk to our Chatbot to narrow down your search.
https://www.symptoma.com/en/ddx/reticulocyte-count-low-to-normal
Reticulocyte Count Lab Test Online - Cura4UReticulocyte Count Lab Test Online - Cura4U
Order reticulocyte count tests from Cura4U at discounted prices! Book your test online from the best labs in USA. Call us at 1-888-360-0001 today!
https://cura4u.com/lab-tests/reticulocyte-count
Reticulocyte count | Guide to Diagnostic TestsReticulocyte count | Guide to Diagnostic Tests
Reticulocyte count answers are found in the Guide to Diagnostic Tests powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
https://im.unboundmedicine.com/medicine/view/GDT/619026/all/Reticulocyte_count
Medical Laboratory and Biomedical Science: Copper deficiency - An unusual cause of anemiaMedical Laboratory and Biomedical Science: Copper deficiency - An unusual cause of anemia
A 56-year-old woman with a complicated medical history presented with nausea, vomiting, neuropathy, and chronic weakness. Complete blood count (Hb 54 g/L, MCV 99 fL, white blood cell count 5.0 × 109/L, neutrophil count 3.5 × 109/L, platelets 374 × 109/L, reticulocyte count 46 × 109/L) showed she was markedly anemic with a normal reticulocyte count ...
http://clinical-laboratory.blogspot.fi/2014/01/copper-deficiency-unusual-cause-of.html
Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture - MORU Tropical...Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture - MORU Tropical...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
https://www.tropmedres.ac/publications/237774
Biblio | College of Public Health and Human Sciences | Oregon State UniversityBiblio | College of Public Health and Human Sciences | Oregon State University
T. Offer, Ho, E., Bruno, R. S., Traber, M., Kuypers, F., and Ames, B. N., A simple assay for frequency of chromosome breaks and loss (Micronuclei) by flow Cytometry of Human Reticulocytes, FASEB Journal, vol. 19, no. 3, pp. 485-7, 2005. ...
https://health.oregonstate.edu/biblio?amp%3Bf%5Bauthor%5D=6971&s=title&o=desc&f%5Bauthor%5D=7701&page=11
Thalassemia Imaging: Practice Essentials, Radiography, Magnetic Resonance ImagingThalassemia Imaging: Practice Essentials, Radiography, Magnetic Resonance Imaging
Thalassemic syndromes are genetically determined disorders of hemoglobin synthesis with decreased production of either alpha or beta polypeptide chains of hemoglobin molecules. This reduced production results from markedly decreased amounts of globin messenger ribonucleic acid.
https://emedicine.medscape.com/article/396792-overview
Reticulocytes, Cellular HbReticulocytes, Cellular Hb
Reticulocytes, Cellular Hb,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories client,medicine,medical supply,medical supplies,medical product
http://www.bio-medicine.org/medicine-products/Reticulocytes--Cellular-Hb-24395-1/
Liquichek™ Reticulocyte Control (C), Level 2 #827 | Clinical Diagnostics | Bio-RadLiquichek™ Reticulocyte Control (C), Level 2 #827 | Clinical Diagnostics | Bio-Rad
Assayed, human whole blood control for reticulocyte counting using Beckman Coulter® VCS instruments and New Methylene Blue stain; level 2 of 3 (4 x 4 mL)
http://www.bio-rad.com/en-us/sku/827liquichek-reticulocyte-control-c-level-2
Arahidonat 15-lipoksigenaza - Википедија, слободна енциклопедијаArahidonat 15-lipoksigenaza - Википедија, слободна енциклопедија
Bryant, R.W., Bailey, J.M., Schewqe, T. and Rapoport, S.M. (1982). „Positional specificity of a reticulocyte lipoxygenase. Conversion of arachidonic acid to 15-S-hydroperoxy-eicosatetraenoic acid. J. Biol. Chem. 257: 6050-6055. PMID 6804460 ...
https://sr.wikipedia.org/wiki/Arahidonat_15-lipoksigenaza
Activity and heat-stability of peptidase activity in cell-free extracts of rabbit reticulocytes and erythrocytes: effects of...Activity and heat-stability of peptidase activity in cell-free extracts of rabbit reticulocytes and erythrocytes: effects of...
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http://www.biochemsoctrans.org/content/10/2/97
Biological characterization of various forms of elongation factor 1 from rabbit reticulocytes. - PubMed - NCBIBiological characterization of various forms of elongation factor 1 from rabbit reticulocytes. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/6568109?dopt=Abstract
R&D Retic gbR&D Retic gb
R&D Retic is a tri-level whole blood reticulocyte control for manual and automated reticulocytes counting methods. Assay values are provided for the manual method and manual with Miller ocular. The target values for the levels are : Level 1 - 1.0%; Level 2 - 5.0% and Level 3 - 10.0%. R&D Retic has 75-day closed vial stability with 14-day open vial stability. Each new lot is shipped every 2 months. R&D Retic exists in 1.5 mL screw cap vial.. ...
https://eurocelldiag.com/r26d-retic-gb.html
Enumeration of Fetal Red Blood Cells, F Cells, and F Reticulocytes in Human Blood - Current Protocols in Cytometry - Davis -...Enumeration of Fetal Red Blood Cells, F Cells, and F Reticulocytes in Human Blood - Current Protocols in Cytometry - Davis -...
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Robust microarray production of freshly expressed proteins in a human milieu. - Early Detection Research Network
In vitro transcription/translation (IVTT) systems are widely used in proteomics. For clinical applications, mammalian systems are preferred for protein folding and activity; however, the level of protein obtained is low. A new system extracted from human cells (1-Step Human Coupled IVT (HCIVT)) has the potential to overcome this problem and deliver high yields of protein expressed in a human milieu.. Western blots and self-assembled protein microarrays were used to test the efficiency of protein synthesis by HCIVT compared to rabbit reticulocyte lysate (RRL). The arrays were also used to measure the immune response obtained from serum of patients exposed to pathogens or vaccine.. HCIVT performed better than RRL in all experiments. The yield of protein synthesized in HCIVT is more than ten times higher than RRL, in both Western blot and protein microarrays. Moreover, HCIVT showed a robust lot-to-lot reproducibility. In immune assays, the signals of many antigens were detected only in ...
https://edrn.jpl.nasa.gov/publications/23027544-robust-microarray-production-of-freshly
Biology-Online •Biology-Online •
We describe a new technique for detecting prenyl anchors in N-terminally glutathione S-transferase (GST)-labeled constructs of target proteins expressed in vitro in rabbit reticulocyte lysate and incubated with 3H-labeled anchor precursors. Alternatively, hemagglutinin (HA)-labeled constructs expressed in vivo (in cell culture) can be used. For registration of the radioactive marker, we propose to use a thin layer chromatography (TLC) analyzer. As a control, the protein yield is tested by Western blotting with anti-GST- (or anti-HA-) antibodies on the same membrane that has been previously used for TLC-scanning. These protocols have been tested with Rap2A, v-Ki-Ras2 and RhoA (variant RhoA63L) including the necessary controls. We show directly that RasD2 is a farnesylation target.. ...
https://www.biology-online.org/kb/revision.php?p_id=9404&a_id=3623
JoVE Author Search: Afanasyev B
Arc, Blood, Blood Cells, Cells, Clone, Clones, Diagnosis, Granulocytes, Incidence, Logistic Regression, Odds Ratio, Patients, Prospective Study, Red Blood Cells, Regression, Regression Analysis, Reticulocyte, Reticulocyte Count, Role, Survival
http://labindex.jove.com/author/Afanasyev-B
net words per minute
Taking the 5 minute test is a good indicator of your speed, but 1 minute is enough for fun. Question: The Distribution Of Net Typing Rate In Words Per Minute For Experienced Typists Can Be Approximated By A Normal Curve With A Mean Of 76 Wpm And A Variance Of 16 Wpm. Alternatively referred to as GWPM (gross words per minute), GWAM is short for gross words a minute and is similar to WPM, except it does not account for any errors.GWAM is the overall typing speed regardless of how many errors you make. This is a good application for users wanting to see how efficient they are over longer periods. It is a measure of the output speed on a keyboard. (Round All Answers To Four Decimal Places.) 1 0. Online English Typing Test. Corrected Relative Net Protein Ratio; corrected reticulocyte percentage (CRP) corrected reticulocyte percentage (CRP) Poll. 60-70 is average. Word processors generally must be able to type from 55 to 90 words per minute. The ability to type at least 40 words per minute , as ...
http://micoimages.com/cold-buffet-cjx/4ef7fd-net-words-per-minute
ReticulocytesReticulocytes
Our Health Library information does not replace the advice of a doctor. Please be advised that this information is made available to assist our patients to learn more about their health. Our providers may not see and/or treat all topics found herein. ...
https://gvog.com/healthwise/?DOCHWID=tp13656
Reticulocyte Testing | BD Biosciences-USReticulocyte Testing | BD Biosciences-US
2019 BD. All rights reserved. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company.. ...
https://www.bdbiosciences.com/us/applications/clinical/transfusiontransplantation/transplantation/reticulocyte-testing/c/790235
rat Metap2 protein Summary Report | CureHunterrat Metap2 protein Summary Report | CureHunter
rat Metap2 protein: from reticulocyte lysates; protects eIF-2 alpha subunit from eIF-2 kinase catalyzed phosphorylation & thus promotes protein synthesis in the presence of eIF-2 kinase; RefSeq NM_022539
http://www.curehunter.com/public/keywordSummaryC489895-rat-Metap2-protein.do
The Hox proteins Ubx and AbdA collaborate with the transcription pausing factor M1BP to regulate gene transcription | The EMBO...
Susanne Wegmann, Bahareh Eftekharzadeh, Katharina Tepper, Katarzyna M Zoltowska, Rachel E Bennett, Simon Dujardin, Pawel R Laskowski, Danny MacKenzie, Tarun Kamath, Caitlin Commins, Charles Vanderburg, Allyson D Roe, Zhanyun Fan, Amandine M Molliex, Amayra Hernandez‐Vega, Daniel Muller, Anthony A Hyman, Eckhard Mandelkow, J Paul Taylor, Bradley T Hyman ...
http://emboj.embopress.org/content/early/2017/09/04/embj.201695751.figures-only
Import of porin is inhibited into tom40 mutant mitochon | Open-iImport of porin is inhibited into tom40 mutant mitochon | Open-i
Import of porin is inhibited into tom40 mutant mitochondria. (A) Radiolabeled porin precursor (10 μl reticulocyte lysate per lane) was incubated with isolated
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2199606_JCB0008032.f8&req=4
Au sujet du comportement différent de divers plasmodiums de mammifères vis-à-vis des réticulocytes<...
TY - JOUR. T1 - Au sujet du comportement différent de divers plasmodiums de mammifères vis-à-vis des réticulocytes. AU - Rodhain, J. AU - Lassman, P. PY - 1939. Y1 - 1939. KW - B780-tropical-medicine. KW - Plasmodium. KW - Protozoology. KW - Laboratory. KW - Reticulocytes. KW - Photographs. M3 - A2: International peer reviewed article (not A1-type). VL - 19. SP - 423. EP - 444. JO - Annales de la Société Belge de Médecine Tropicale. JF - Annales de la Société Belge de Médecine Tropicale. SN - 0365-6527. ER - ...
https://research.itg.be/en/publications/au-sujet-du-comportement-diff%C3%A9rent-de-divers-plasmodiums-de-mammi
ReticulocyteReticulocyte
Calculating the reticulocyte production index is an important step in understanding whether or not the reticulocyte count is ... Reticulocytes are a valuable tool for biologists who study protein translation. Reticulocytes are unusual among cells in that ... Reticulocytes are immature red blood cells (RBCs). In the process of erythropoiesis (red blood cell formation), reticulocytes ... Like mature red blood cells, in mammals, reticulocytes do not have a cell nucleus. They are called reticulocytes because of a ...
https://en.wikipedia.org/wiki/Reticulocyte
Reticulocyte production indexReticulocyte production index
The reticulocyte production index (RPI), also called a corrected reticulocyte count (CRC), is a calculated value used in the ... This has the effect of raising the reticulocyte count simply because reticulocytes produced on any single day will spend more ... In anemia, the patient's red blood cells are depleted, creating an erroneously elevated reticulocyte count. Reticulocytes are ... is the medical term for an abnormal decrease of reticulocytes in the body The reticulocyte production index may find new use as ...
https://en.wikipedia.org/wiki/Reticulocyte_production_index
Reticulocyte binding protein homologsReticulocyte binding protein homologs
This family is named after the reticulocyte binding proteins in P. vivax, a parasite that only infects reticulocytes (immature ... Reticulocyte binding protein homologs (RHs) are a superfamily of proteins found in Plasmodium responsible for cell invasion. ... March 2011). "Reticulocyte and erythrocyte binding-like proteins function cooperatively in invasion of human erythrocytes by ... "Structural characterization of the erythrocyte binding domain of the reticulocyte binding protein homologue family of ...
https://en.wikipedia.org/wiki/Reticulocyte_binding_protein_homologs
PolychromasiaPolychromasia
The absolute number of reticulocytes is referred to as the reticulocyte index and is calculated by adjusting the reticulocyte ... If a low count of reticulocytes is found, it usually indicates bone marrow stress. If a high reticulocyte count is found, it is ... Most red blood cells are released into the blood as reticulocytes. Polychromasia occurs when the immature reticulocytes of the ... These young red blood cells are commonly called reticulocytes. All polychromatophilic cells are reticulocytes, however, not all ...
https://en.wikipedia.org/wiki/Polychromasia
Pappenheimer bodiesPappenheimer bodies
Reticulocytes often contain Pappenheimer bodies. They are mostly observed in diseases such as Myelodysplastic syndrome (MDS), ...
https://en.wikipedia.org/wiki/Pappenheimer_bodies
TMEM248TMEM248
A Geo Profile in NCBI shows expression of TMEM248 is much higher in fetal reticulocytes than in adult reticulocytes. ... Reticulocytes are immature red blood cells. GWAS studies found that TMEM248 SNP rs4718428 is associated with corneal topography ... "TMEM248 - Fetal and adult reticulocytes (HG-U133B)". National Center for Biotechnology Information. National Library of ...
https://en.wikipedia.org/wiki/TMEM248
TransfectionTransfection
Purification and characterization of a double-stranded RNA-dependent protein synthesis inhibitor from reticulocyte lysates". J ... "Characterization of purified double-stranded RNA-activated eIF-2 alpha kinase from rabbit reticulocytes". J Biol Chem. 256 (14 ...
https://en.wikipedia.org/wiki/Transfection
New methylene blueNew methylene blue
"Reticulocyte Count" (PDF). Prentice-Hall. (Articles with short description, Short description is different from Wikidata, ...
https://en.wikipedia.org/wiki/New_methylene_blue
Clement FinchClement Finch
... human deferric transferrin's interaction with reticulocytes, the uptake and release of iron in human transferrin, rat ... "Competitive Advantage of Diferric Transferrin in Delivering Iron to Reticulocytes". Proceedings of the National Academy of ... "Interaction of Human Diferric Transferrin with Reticulocytes". Proceedings of the National Academy of Sciences. 78 (1): 621-625 ...
https://en.wikipedia.org/wiki/Clement_Finch
ReticulocytopeniaReticulocytopenia
... is the medical term for an abnormal decrease of reticulocytes in the body. Reticulocytes are new, immature ...
https://en.wikipedia.org/wiki/Reticulocytopenia
C16orf82C16orf82
Goh SH, Josleyn M, Lee YT, Danner RL, Gherman RB, Cam MC, Miller JL (July 2007). "The human reticulocyte transcriptome". ...
https://en.wikipedia.org/wiki/C16orf82
History of RNA biologyHistory of RNA biology
This problem was overcome in the 1960s by the use of reticulocytes in vertebrates, which produce large quantities of mRNA that ... Lamfrom H, McLaughlin CS, Sarabhai A (1966). "Direction of reading the genetic message in reticulocytes". J. Mol. Biol. 22 (2 ...
https://en.wikipedia.org/wiki/History_of_RNA_biology
Micrococcal nucleaseMicrococcal nuclease
Preparation of rabbit reticulocyte lysates. Studies of chromatin structure. Removal of nucleic acids from laboratory protein ...
https://en.wikipedia.org/wiki/Micrococcal_nuclease
Exosome (vesicle)Exosome (vesicle)
Exosomes were first discovered in the maturing mammalian reticulocyte (immature red blood cell) by Stahl and group in 1983 and ... Exosomes were shown to participate in selective removal of many plasma membrane proteins as the reticulocyte becomes a mature ... Harding C, Stahl P (June 1983). "Transferrin recycling in reticulocytes: pH and iron are important determinants of ligand ... Pan BT, Johnstone RM (July 1983). "Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: ...
https://en.wikipedia.org/wiki/Exosome_(vesicle)
MitochondrionMitochondrion
Ney PA (May 2011). "Normal and disordered reticulocyte maturation". Current Opinion in Hematology. 18 (3): 152-157. doi:10.1097 ...
https://en.wikipedia.org/wiki/Mitochondrion
13-Hydroxyoctadecadienoic acid13-Hydroxyoctadecadienoic acid
Kühn, H; Brash, A. R (1990). "Occurrence of lipoxygenase products in membranes of rabbit reticulocytes. Evidence for a role of ... In the maturation of the red blood cell lineage (see erythropoiesis) from mitochondria-bearing reticulocytes to mature ... and 13-keto-octadecadienoic acid in biological membranes oxygenated by the reticulocyte lipoxygenase". Archives of Biochemistry ... "Substrate Specificity Changes for Human Reticulocyte and Epithelial 15-Lipoxygenases Reveal Allosteric Product Regulation". ...
https://en.wikipedia.org/wiki/13-Hydroxyoctadecadienoic_acid
Translation (biology)Translation (biology)
... time-dependent processes and application to reticulocytes". Journal of Theoretical Biology. 86 (2): 279-313. Bibcode:1980JThBi ...
https://en.wikipedia.org/wiki/Translation_(biology)
ALOX15ALOX15
Bryant RW, Bailey JM, Schewe T, Rapoport SM (June 1982). "Positional specificity of a reticulocyte lipoxygenase. Conversion of ... Human ALOX15 protein is highly expressed in circulating blood eosinophils and reticulocytes, cells, bronchial airway epithelial ... identity to the reticulocyte enzyme and expression in epithelium". The American Journal of Physiology. 262 (4 Pt 1): L392-8. ... "Occurrence of the erythroid cell specific arachidonate 15-lipoxygenase in human reticulocytes". Biochemical and Biophysical ...
https://en.wikipedia.org/wiki/ALOX15
Left shift (medicine)Left shift (medicine)
84-84 Leukocytosis Reticulocyte Kumar, Vinay; Abbas, Abul K.; Fausto, Nelson; Robbins, Stanley Leonard; Cotran, Ramzi S. (30 ... when increased reticulocytes and immature erythrocyte-precursor cells appear in the peripheral circulation. The standard ...
https://en.wikipedia.org/wiki/Left_shift_(medicine)
Tim HuntTim Hunt
His PhD was supervised by Asher Korner and focused on haemoglobin synthesis in intact rabbit reticulocytes (immature red blood ... Pelham, Hugh R. B. (1978). Transcription and Translation in Reticulocyte Lysates. lib.cam.ac.uk (PhD thesis). University of ... While there, they discovered that tiny amounts of glutathione inhibited protein synthesis in reticulocytes and that tiny ... Ehrenfeld, E.; Hunt, T. (1971). "Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte ...
https://en.wikipedia.org/wiki/Tim_Hunt
Cell-free systemCell-free system
Rabbit reticulocytes have been lysed in a solution of MgCl2 and had the extract filtered away from the membranes by ... E. coli, wheat germ, and rabbit reticulocytes have all proven useful to create cell-free systems by extraction of their ... Woodward, William R.; Ivey, Joel L.; Herbert, Edward (1974). "[67a] Protein synthesis with rabbit reticulocyte preparations". ...
https://en.wikipedia.org/wiki/Cell-free_system
EIF4A1EIF4A1
It was first characterized in 1982 by Grifo, et al., who purified it from rabbit reticulocyte lysate. The regulation of the ...
https://en.wikipedia.org/wiki/EIF4A1
Brilliant cresyl blueBrilliant cresyl blue
... is a supravital stain used for counting reticulocytes. It is classified as an oxazine dye. N95 dust masks ...
https://en.wikipedia.org/wiki/Brilliant_cresyl_blue
Transferrin receptorTransferrin receptor
"Transfer of iron from serum iron-binding protein to human reticulocytes". The Journal of Clinical Investigation. 38 (1, Part 1 ...
https://en.wikipedia.org/wiki/Transferrin_receptor
15-Hydroxyeicosatetraenoic acid15-Hydroxyeicosatetraenoic acid
Schewe, T; Halangk, W; Hiebsch, C; Rapoport, S. M. (1975). "A lipoxygenase in rabbit reticulocytes which attacks phospholipids ... Identity to the reticulocyte enzyme and expression in epithelium". The American Journal of Physiology. 262 (4 Pt 1): L392-8. ...
https://en.wikipedia.org/wiki/15-Hydroxyeicosatetraenoic_acid
Plasmodium eylesiPlasmodium eylesi
The parasite prefers to infect reticulocytes but will infect older erythrocytes. Multiple infections like those of Plasmodium ...
https://en.wikipedia.org/wiki/Plasmodium_eylesi
ErythropoiesisErythropoiesis
By the reticulocyte stage, the cell has extruded its nucleus, but is still capable of producing hemoglobin. Essential for the ... As reticulocytes lose their polyribosomes, they become mature red blood cells. Sherwood, L, Klansman, H, Yancey, P: Animal ... At this stage the nucleus is expelled before the cell becomes a reticulocyte. (These cells still contain RNA and are also ... reticulocytes. After one to two days, these ultimately become "erythrocytes" or mature red blood cells. These stages correspond ...
https://en.wikipedia.org/wiki/Erythropoiesis
Hugh PelhamHugh Pelham
Pelham, Hugh R. B. (1978). Transcription and Translation in Reticulocyte Lysates (PhD thesis). University of Cambridge. OCLC ... Reticulocytes). His PhD was supervised by Richard J. Jackson and Tim Hunt, who went on to receive the Nobel Prize in Physiology ... "An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates". European Journal of Biochemistry. 67 (1): 247-256. ...
https://en.wikipedia.org/wiki/Hugh_Pelham
Shine-Dalgarno sequenceShine-Dalgarno sequence
The 3'-termini of rabbit reticulocyte ribosomal RNA". Biochemical Journal. 120 (2): 353-363. doi:10.1042/bj1200353. PMC 1179605 ...
https://en.wikipedia.org/wiki/Shine–Dalgarno_sequence
Asher KornerAsher Korner
Munro, A. J.; Korner, A. (1963). "Lack of Messenger RNA in Reticulocyte Cell-sap". Nature. 198 (4883): 891-892. doi:10.1038/ ...
https://en.wikipedia.org/wiki/Asher_Korner
Reticulocytes: MedlinePlus Medical Encyclopedia ImageReticulocytes: MedlinePlus Medical Encyclopedia Image
Reticulocytes normally make up 1% of the total RBC count, but may exceed levels of 4% when compensating for anemia. ... These slightly immature cells are called reticulocytes, and are characterized by a network of filaments and granules. ... These slightly immature cells are called reticulocytes, ...
https://medlineplus.gov/ency/imagepages/1491.htm
mean reticulocyte volumemean reticulocyte volume
... Go to external page http://www.ebi.ac.uk/efo/EFO_0010701 Copy ...
https://www.ebi.ac.uk/ols/ontologies/efo/terms?short_form=EFO_0010701
Browsing by Subject ReticulocytesBrowsing by Subject "Reticulocytes"
ICSH guidelines for reticulocyte counting by microscopy on supravitally stained preparations / prepared on behalf of the World ...
https://extranet.who.int/iris/restricted/browse?authority=Reticulocytes&type=mesh
Cold Agglutinin Disease Workup: Approach Considerations, Complete Blood Cell Count and Peripheral Smear, Reticulocytes and...Cold Agglutinin Disease Workup: Approach Considerations, Complete Blood Cell Count and Peripheral Smear, Reticulocytes and...
Reticulocytes and Spherocytes. The results of the reticulocyte count are usually increased in patients with cold agglutinin ...
https://www.medscape.com/answers/135327-120828/what-is-the-role-of-blood-tests-in-the-workup-of-cold-agglutinin-disease
Cold Agglutinin Disease Workup: Approach Considerations, Complete Blood Cell Count and Peripheral Smear, Reticulocytes and...Cold Agglutinin Disease Workup: Approach Considerations, Complete Blood Cell Count and Peripheral Smear, Reticulocytes and...
Reticulocytes and Spherocytes. The results of the reticulocyte count are usually increased in patients with cold agglutinin ...
https://emedicine.medscape.com/article/135327-workup
JCI - Selective reticulocyte destruction in erythrocyte pyruvate kinase deficiencyJCI - Selective reticulocyte destruction in erythrocyte pyruvate kinase deficiency
Those reticulocytes with particularly low levels of PK exhibit very low glycolytic rates and thus are uniquely reliant upon ... Other reticulocytes, better endowed with PK activity, can meet the increased ATP requirements of young erythrocytes. Upon ... PK-deficient reticulocytes, dependent upon oxidative phosphorylation for ATP production, are exquisitely sensitive to cyanide- ... in a patient with erythrocyte PK deficiency have provided evidence for prompt reticulocyte sequestration and destruction by the ...
https://www.jci.org/articles/view/106539/scanned-page/697
Reticulocyte CountReticulocyte Count
The reticulocyte count is given as the percentage of red blood cells that are reticulocytes (the number of reticulocytes ... Reticulocyte Count. Test Overview. A reticulocyte count is a blood test that measures how fast red blood cells called ... A reticulocyte count is a blood test that measures how fast red blood cells called reticulocytes are made by the bone marrow ... A reticulocyte count is done to:. *See whether anemia is caused by fewer red blood cells being made or by a greater loss of red ...
https://myhealth.alberta.ca/health/Pages/conditions.aspx?hwid=hw203366
Lab Test: Reticulocyte Count, Automated | Akron Childrens HospitalLab Test: Reticulocyte Count, Automated | Akron Children's Hospital
While we are ranked among the best childrens hospitals in the country, its our compassionate approach to treatment that makes us truly exceptional. Through a combination of revolutionary treatments and extraordinary patient experiences, our care does more than heal. It brings the entire family together for emotional support and understanding across multiple locations to reach you in the community where you live. Learn more... ...
https://www.akronchildrens.org/lab_tests/Reticulocyte-Count-Automated.html
Hematopoiesis and Bone Marrow Histology - Reticulocytes - histology slideHematopoiesis and Bone Marrow Histology - Reticulocytes - histology slide
Reticulocytes - histology slide This histology slide shows reticulocytes.. Image source: http://www.hematologyatlas.com/ ...
http://histology-world.com/photoalbum/displayimage.php?album=65&pid=3786
Start Over...Start Over...
A calculator for determining absolute reticulocyte count (ARC). ... Close more info about Absolute Reticulocyte Count Calculator ...
https://www.oncologynurseadvisor.com/home/resources/medical-calculators/absolute-reticulocyte-count-calculator/
Paper: Single-Cell RNA Sequencing of Sickle Cell Reticulocytes to Identify Beta-Globin Genotypes and Associated Gene Expression...Paper: Single-Cell RNA Sequencing of Sickle Cell Reticulocytes to Identify Beta-Globin Genotypes and Associated Gene Expression...
However, reticulocytes are immature RBCs that still contain some RNA and could be used to evaluate allelic correction. We ... Methods: Reticulocytes were sorted from peripheral blood from healthy donors (AA), donors with sickle cell trait (AS), and ... 3468 Single-Cell RNA Sequencing of Sickle Cell Reticulocytes to Identify Beta-Globin Genotypes and Associated Gene Expression ... method to enable enumeration of potential gene-editing outcomes in peripheral reticulocytes. ...
https://ash.confex.com/ash/2022/webprogram/Paper162370.html
Validation of the reticulocyte channel of Sysmex XN-9000 system for blood cell count in samples with suspected cold...Validation of the reticulocyte channel of Sysmex XN-9000 system for blood cell count in samples with suspected cold...
Validation of the reticulocyte channel of Sysmex XN-9000 system for blood cell count in samples with suspected cold ... Validation of the reticulocyte channel of Sysmex XN-9000 system for blood cell count in samples with suspected cold ... Validation of the reticulocyte channel of Sysmex XN-9000 system for blood cell count in samples with suspected cold ... Correction: Validation of the reticulocyte channel of Sysmex XN-9000 system for blood cell count in samples with suspected cold ...
https://jcp.bmj.com/content/73/12/847.info
Genetic Diversity in New Members of the Reticulocyte Binding Protein Family in Thai Plasmodium vivax Isolates - Nuffield...
Genetic Diversity in New Members of the Reticulocyte Binding Protein Family in Thai Plasmodium vivax Isolates Share Share Share ... Genetic Diversity in New Members of the Reticulocyte Binding Protein Family in Thai Plasmodium vivax Isolates ...
https://www.ndm.ox.ac.uk/publications/316299
reticulocyte-count-test - Jagsan Diagnostics & Clinicsreticulocyte-count-test - Jagsan Diagnostics & Clinics
Address: 1-1-74/A 1ST FLOOR TAMASI AVENUE STREET NO 10 GANDHI NAGAR KAVADIGUDA ...
https://www.jagsandiagnostics.com/reticulocyte-count-test/
Best Labs and Diagnostic Centers for Reticulocyte Count in New Delhi | 1mgBest Labs and Diagnostic Centers for Reticulocyte Count in New Delhi | 1mg
Find the best Labs and Diagnostic Centers for Reticulocyte Count Online in New Delhi. View its pre-requirement, prices and user ...
https://www.1mg.com/labs/bhubaneswar/reticulocyte-count-1892-p
Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax cultureHematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture
... DSpace/Manakin Repository. ... Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. Noulin, F.; Manesia, J. K.; ... As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a ... Reticulocytes. Red blood cells. Hematopoietic stem cells. Peripheral blood. Bone marrow. CD34+. Laboratory techniques and ...
http://dspace.itg.be/handle/10390/8262
Glyceraldehyde-3-phosphate dehydrogenase is one of the three major RNA-binding proteins of rabbit reticulocytes<...Glyceraldehyde-3-phosphate dehydrogenase is one of the three major RNA-binding proteins of rabbit reticulocytes<...
Glyceraldehyde-3-phosphate dehydrogenase is one of the three major RNA-binding proteins of rabbit reticulocytes. FEBS Letters. ... N2 - One of the 3 major RNA-binding proteins of rabbit reticulocytes, a polypeptide of 36 kDa, is identified as glyceraldehyde- ... AB - One of the 3 major RNA-binding proteins of rabbit reticulocytes, a polypeptide of 36 kDa, is identified as glyceraldehyde- ... One of the 3 major RNA-binding proteins of rabbit reticulocytes, a polypeptide of 36 kDa, is identified as glyceraldehyde-3- ...
https://www.researchwithrutgers.com/en/publications/glyceraldehyde-3-phosphate-dehydrogenase-is-one-of-the-three-majo
Reticulocytes - South West London PathologyReticulocytes - South West London Pathology
Reticulocytes may be requested on samples up to 72 hours old. Lab Tests Online UK. Lab Tests Online - UK is a website written ...
https://www.swlpath.nhs.uk/tests-database/reticulocytes/
ReticulocytesReticulocytes
... Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL ...
http://atlas.centralx.com/p/image/cells/blood-cells/erythrocytes/reticulocytes/
Reticulocyte Production Index - AccuteqsReticulocyte Production Index - Accuteqs
Reticulocyte Production Index Deprecated: Function get_magic_quotes_gpc() is deprecated in /home/ozoei4ngo532/public_html/wp- ...
https://accuteqs.com.ph/reticulocyte-production-index/
Reticulocyte Enumeration: Past & Present - HABX
Reticulocyte Enumeration: Past & Present. By Roger S. Riley, MD, PhD, Jonathan M. Ben-Ezra, MD, Ann Tidwell, MT (ASCP), SH- ... automated techniques of reticulocyte enumeration are more precise, accurate, objective, and cost-effective, since 30,000 or ... Reticulocyte Parameters and Hemoglobin F Production in Sickle Cell Disease Patients Undergoing Hydroxyurea Therapy. ... Immature reticulocyte fraction as a useful parameter for blood transfusion assessment in anaemia. ...
https://habx.in/details/Coagulation/Webinars/Reticulocyte_Enumeration:_Past_&_Present
Reticulocyte Count - Ascend Reference ManualReticulocyte Count - Ascend Reference Manual
Avoid hemolysis or clotted specimen. Specimen must be received in the laboratory within 48 hours of collection. Do not freeze. ...
https://referencemanual.aclab.com/testarticle/reticulocyte-count/
CDC - Malaria - References and Resources - Interactive Training - Clinical Case Study 3CDC - Malaria - References and Resources - Interactive Training - Clinical Case Study 3
Other laboratory findings at that time include hemoglobin 8.6 g/dL; reticulocytes 2.4%; total bilirubin 6.2 mg/dL; LDH 444 U/L ...
https://www.cdc.gov/malaria/references_resources/interactive_training/cc-3/index.html
You searched for /?keywords=Anemia - Testing.comYou searched for /?keywords=Anemia - Testing.com
Reticulocytes are immature red blood cells (RBCs). A reticulocyte test counts them to help evaluate anemia or bone marrow ...
https://www.testing.com/?s=/?keywords=Anemia
Reticulocyte Count | Reticulocyte Count Specialist | Mya CareReticulocyte Count | Reticulocyte Count Specialist | Mya Care
A Reticulocytes count blood test is ordered to evaluate the bone marrows ability to produce red blood cells Click to read more ... A Reticulocytes count blood test is ordered to evaluate the bone marrows ability to produce red blood cells (RBCs); to help ...
https://myacare.com/procedure/reticulocyte-count
Reticulocyte Panel « The Pathology Laboratory - Lake Charles, LA
This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognizing you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.. You can adjust all of your cookie settings by navigating the tabs on the left hand side.. This Website is based in the United States and the information we collect is governed by U.S. law. By accessing or using the Website or otherwise providing information to us, you consent to the processing, transfer, and storage of information to the U.S., where you may not have the same rights and protections as you do under local law.. Privacy Policy. ...
https://thepathlab.com/tests/reticulocyte-panel/
α|inf|4|/inf|β|inf|1|/inf|-Integrin expression on sickle reticulocytes: Vascular cell adhesion molecule-1-dependent binding to...α|inf|4|/inf|β|inf|1|/inf|-Integrin expression on sickle reticulocytes: Vascular cell adhesion molecule-1-dependent binding to...
In patients with sickle cell anemia, up to 25% of reticulocytes express the α4β1-integrin complex. Furthermore, erythrocytes ... Integrin expression on sickle reticulocytes: Vascular cell adhesion molecule-1-dependent binding to endothelium Academic ... binding of α4β1-expressing reticulocytes to cytokine-activated endothelial cells may initiate vascular complications in sickle ...
https://scholars.uab.edu/display/pub16845
Table - Babesia microti Causing Intravascular Hemolysis in Immunocompetent Child, China - Volume 29, Number 3-March 2023 -...
Reticulocytes. 0.5%-2.5%. 2.07%. Clotting function. NA. Normal. Bilirubin, μmol/L. Total. 3.42-20.5. 55.98. ...
https://wwwnc.cdc.gov/eid/article/29/3/22-0888-t1
High Reticulocyte Count Explained 101 [Back To Basics] - IHTHigh Reticulocyte Count Explained 101 [Back To Basics] - IHT
If a doctor has determined a high reticulocyte count, it is likely due to blood loss. Questions must be asked why the count is ... The Reticulocyte Production Index. This index is used to determine how quickly reticulocytes are formed, which is essential ... Protein Translation And Reticulocytes. Reticulocytes can process protein just like a regular red blood cell in most cases. ... How To Find Reticulocytes. You are looking for signals that they can admit which will not be as strong as a typical lymphocyte ...
https://www.ihtbio.com/high-reticulocyte-count-explained/
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Reticulocytes [‎1]‎. Retina [‎1]‎. Retinal Artery Occlusion [‎1]‎. Retinal Dystrophies [‎1]‎. Retirement [‎1]‎. ...
https://apps.who.int:80/iris/browse?rpp=20&offset=5128&etal=-1&sort_by=-1&type=mesh&starts_with_previous=R&order=ASC
CountsRBCsCountPeripheral bloodPlasmodiumErythropoietinSickle cell aCellsBilirubinBoneHemolysisAssayMaturationEnumerationRabbitVivaxERYTHROCYTESIron deficiencyAdultsAnemiaMalariaProteinMethodsAbsenceLevelsHighNormalMechanismPatients
Counts8
  • Also, being at high altitudes may cause reticulocyte counts to rise, to help you adjust to the lower oxygen levels at high altitudes. (alberta.ca)
  • A reticulocyte test counts them to help evaluate anemia or bone marrow function. (testing.com)
  • The absolute reticulocyte counts for the anemic patients were significantly higher than for those without anemia on Days 0, 7, 21, and 28. (who.int)
  • Elevations in liver enzymes and decreases in platelet counts and reticulocytes were also observed with the conventional ADC at 20 and 60mg/kg treatments, whereas these effects were only seen with the highest dose of the SMARTag ADC. (catalent.com)
  • However, the MCV also may be elevated in patients with high reticulocyte counts since these cells are larger than mature RBCs. (medscape.com)
  • Due to microangiopathic hemolytic anemia, patients typically have very high LDH, indirect bilirubin, and reticulocyte counts. (cooperhealth.org)
  • Conclusion: This study provides locally derived ranges for complete blood cell and reticulocyte counts for a healthy adult population in Yaoundé, Cameroon. (bvsalud.org)
  • The cut-off points were reticulocyte counts above ten per cent and alkaline phosphatase levels above 13 K.A. units. (ac.ke)
RBCs3
  • However, reticulocytes are immature RBCs that still contain some RNA and could be used to evaluate allelic correction. (confex.com)
  • Reticulocytes are immature red blood cells (RBCs). (testing.com)
  • They are sometimes called RBCs, but they are also called reticulocytes. (ihtbio.com)
Count19
  • Reticulocytes normally make up 1% of the total RBC count, but may exceed levels of 4% when compensating for anemia. (medlineplus.gov)
  • The results of the reticulocyte count are usually increased in patients with cold agglutinin disease, with polychromasia in the peripheral blood smear. (medscape.com)
  • A reticulocyte count is a blood test that measures how fast red blood cells called reticulocytes are made by the bone marrow and released into the blood. (alberta.ca)
  • The reticulocyte count rises when there is a lot of blood loss or in certain diseases in which red blood cells are destroyed prematurely, such as hemolytic anemia . (alberta.ca)
  • For example, a higher reticulocyte count means that iron replacement treatment or other treatment to reverse the anemia is working. (alberta.ca)
  • The reticulocyte count is given as the percentage of red blood cells that are reticulocytes (the number of reticulocytes divided by the total number of red blood cells, multiplied by 100). (alberta.ca)
  • A high reticulocyte count may mean more red blood cells are being made by the bone marrow. (alberta.ca)
  • The reticulocyte count rises after the treatment for pernicious anemia , iron deficiency anemia , or folic acid deficiency anemia starts working. (alberta.ca)
  • A low reticulocyte count can also be caused by exposure to radiation, a long-term (chronic) infection, or by certain medicines that damage the bone marrow. (alberta.ca)
  • If a doctor has determined a high reticulocyte count , it is likely due to blood loss. (ihtbio.com)
  • By measuring the amount of bone marrow, you have, combined with the reticulocyte count, they can determine if this is either normal or if you have far too many. (ihtbio.com)
  • What Does A High Reticulocyte Count Mean? (ihtbio.com)
  • Simultaneously, there will also be a high reticulocyte count , which indicates the replacement work. (ihtbio.com)
  • Serum samples were taken on admission (Day 0), then on Days 7, 21, and 28, to measure the reticulocyte count, absolute reticulocyte count, reticulocyte hemoglobin content, and erythropoietin level (Epo). (who.int)
  • The sensitivity and specificity were 40% and 98% respectively for reticulocyte count and 80% and 90% respectively for reticulocyte index. (who.int)
  • An increased reticulocyte count represents increased RBC production and is a criterion for hemolysis but is not specific for hemolysis. (medscape.com)
  • The reticulocyte count may be normal or low in patients with bone marrow suppression despite ongoing severe hemolysis (aplastic crisis). (medscape.com)
  • We therefore studied the role of clinical (Body Mass Index), haematological (reticulocyte count, haemoglobin level), and biochemical (serum bilirubin: direct and indirect, serum alkaline phosphatase, serum transaminase) indices in predicting sickle cell anaemia patients likely to develop gallstones. (ac.ke)
  • Body mass index (BMI), reticulocyte count and alkaline phosphatase were found to have a significant positive association with increased likelihood of gallstone formation at p values of 0.004, 0.007 and 0.007, respectively. (ac.ke)
Peripheral blood5
  • Reticulocytes were sorted from peripheral blood from healthy donors (AA), donors with sickle cell trait (AS), and donors with sickle cell disease (SS) by fluorescence-activated cell sorting (FACS) based on a Live/CD235a+/CD45-/CD71+/TO+ phenotype and mixed at given ratios before application to the 10x genomics workflow for scRNAseq. (confex.com)
  • We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). (itg.be)
  • The enumeration of peripheral blood reticulocytes is often performed to obtain information about the functional integrity of the bone marrow. (habx.in)
  • Reticulocytosis (an increased number of peripheral blood reticulocytes) occurs in anemic patients with functional bone marrow while anemic patients with dysfunctional bone marrow produce decreased numbers of reticulocytes, and have decreased numbers of peripheral blood reticulocytes (i.e., reticulocytopenia) in the absence of extramedullary hematopiesis and other factors. (habx.in)
  • We found that reticulocyte numbers and peripheral blood transcriptional signatures robustly correlated with disease severity. (openrepository.com)
Plasmodium3
  • The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. (itg.be)
  • IMSEAR at SEARO: Defective erythropoietin production and reticulocyte response in acute Plasmodium falciparum malaria-associated anemia. (who.int)
  • Leowattana W, Krudsood S, Tangpukdee N, Brittenham G, Looareesuwan S. Defective erythropoietin production and reticulocyte response in acute Plasmodium falciparum malaria-associated anemia. (who.int)
Erythropoietin1
  • To elucidate the relationship between falciparum malaria-associated anemia and serum erythropoietin (Epo) levels and reticulocyte response during acute malaria infection, 87 adults aged 18-65 years presenting with acute, uncomplicated malaria were examined on enrollment and for 28 days of follow-up. (who.int)
Sickle cell a2
  • In patients with sickle cell anemia, up to 25% of reticulocytes express the α4β1-integrin complex. (uab.edu)
  • Thus, binding of α4β1-expressing reticulocytes to cytokine-activated endothelial cells may initiate vascular complications in sickle cell anemia and perhaps other hemolytic anemias during episodes of infection and inflammation. (uab.edu)
Cells9
  • These slightly immature cells are called reticulocytes, and are characterized by a network of filaments and granules. (medlineplus.gov)
  • Reticulocytes are in the blood for about 2 days before developing into mature red blood cells. (alberta.ca)
  • In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. (itg.be)
  • automated techniques of reticulocyte enumeration are more precise, accurate, objective, and cost-effective, since 30,000 or more cells can be accurately evaluated in a very short period of time. (habx.in)
  • They can derive the fraction of blood cells to reticulocytes from this information. (ihtbio.com)
  • Immature reticulocytes fraction-high (IRF-H) analysis measures cells that have recently entered the circulation from the bone marrow. (labcorp.com)
  • We have used 31 P, 23 Na and 19 F nuclear magnetic resonance (NMR) spectroscopy to compare the intracellular free ion and phosphometabolite levels in guinea pig reticulocytes and mature red blood cells. (elsevier.com)
  • Sizeable but opposite changes in intracellular sodium (5.5 μmol/ml cells in reticulocytes vs. 8.5 μmol/ml cells in erythrocytes) and intracellular free calcium (99 nM vs. 31 nM in reticulocytes and mature red cells, respectively) were also observed, suggesting that alterations in the kinetics of membrane ion transport systems, accompanying changes in phospholipid and cholesterol content, occur during the process of red cell maturation. (elsevier.com)
  • However, in contrast to dog red blood cells, there was no evidence for the presence of a Na + /Ca 2+ exchanger in guinea pig reticulocytes or erythrocytes. (elsevier.com)
Bilirubin1
  • Radioisotope studies of bilirubin turnover, ferrokinetics, and red cell survival (51Cr) in a patient with erythrocyte PK deficiency have provided evidence for prompt reticulocyte sequestration and destruction by the reticuloendothelial system. (jci.org)
Bone1
  • The other thing to consider is bone marrow activity in conjunction with the reticulocytes. (ihtbio.com)
Hemolysis1
  • In addition to hemolysis, increased reticulocytes may be a response to blood loss or the treatment of iron, vitamin B12, or folate deficiencies. (medscape.com)
Assay1
  • Original collection time of sample used for 'Reticulocytes Number' assay (blood sample obtained from UK Biobank assessment centre visit). (ox.ac.uk)
Maturation4
  • During the maturation process reticulocytes lose their intracellular organelles and undergo changes in membrane lipid composition and ion transport properties. (elsevier.com)
  • While several reports indicate differences in the levels of magnesium, sodium and calcium in reticulocytes and erythrocytes, controversy remains concerning the actual magnitude and direction of ionic alterations during reticulocyte maturation. (elsevier.com)
  • In contrast to a sharply decreased concentration of ATP in erythrocytes in comparison to reticulocytes, the intracellular free magnesium, measured using 31 P-NMR, was increased by about 65% upon maturation (150 μmol/l cell water in reticulocytes in comparison to 250 μmol/l cell water in erythrocytes). (elsevier.com)
  • NIX is required for programmed mitochondrial clearance during reticulocyte maturation. (nature.com)
Enumeration2
  • We sought to develop a single-cell RNA sequencing (scRNAseq) method to enable enumeration of potential gene-editing outcomes in peripheral reticulocytes. (confex.com)
  • A variety of RNA-specific fluorescent dyes have been utilized for automated reticulocyte enumeration, and some hematology analyzers utilize optical light scatter analysis to perform reticulocyte analysis on specimens stained with new methylene blue or other dyes. (habx.in)
Rabbit4
  • One of the 3 major RNA-binding proteins of rabbit reticulocytes, a polypeptide of 36 kDa, is identified as glyceraldehyde-3-phosphate dehydrogenase (GAPD). (researchwithrutgers.com)
  • Ryazanov, AG 1985, ' Glyceraldehyde-3-phosphate dehydrogenase is one of the three major RNA-binding proteins of rabbit reticulocytes ', FEBS Letters , vol. 192, no. 1, pp. 131-134. (researchwithrutgers.com)
  • We find that in crude preparations from rabbit reticulocyte lysates, hemin inhibits the conversion of proinhibitor to inhibitor catalyzed by endogenous cAMP-dependent protein kinase upon addition of cAMP, but not that caused by the addition of free protein kinase catalytic subunit. (ias.ac.in)
  • Use of purified polysomes from rabbit reticulocytes in a specific test for initiation factors. (openrepository.com)
Vivax3
  • Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. (itg.be)
  • As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies. (itg.be)
  • Using Brazilian and Thai clinical isolates, data show that TfR1-PvRBP2b invasion pathway is critical for the recognition of reticulocytes during P. vivax invasion. (semanticscholar.org)
ERYTHROCYTES1
  • Other reticulocytes, better endowed with PK activity, can meet the increased ATP requirements of young erythrocytes. (jci.org)
Iron deficiency1
  • Reticulocyte haemoglobin content (RHC) is an early biomarker of iron deficiency or restriction, which also demonstrates response to iron therapy. (nevinmanimala.com)
Adults1
  • It can range up to 2.5% in adults, yet an infant's reticulocytes can be as high as 6% . (ihtbio.com)
Anemia1
  • This index is used to determine how quickly reticulocytes are formed, which is essential information, especially since higher than typical values can represent having anemia. (ihtbio.com)
Malaria1
  • These results indicate defective Epo production and reticulocyte response in adult patients suffering from acute P. falciparum malaria, which differs from pediatric patients. (who.int)
Protein3
  • Reticulocytes can process protein just like a regular red blood cell in most cases. (ihtbio.com)
  • The formation of translational inhibitor (active eIF-2 kinase) from proinhibitor (inactive eIF-2 kinase) in reticulocyte lysates, known to be controlled by hemin, can, as we recently reported, be induced by 3':5'-cyclic AMP(cAMP)-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) or its catalytic subunit. (ias.ac.in)
  • The mechanism by which hemin prevents the formation of the inhibitor and maintains protein synthesis in reticulocyte lysates is thus explained. (ias.ac.in)
Methods1
  • METHODS: Effectiveness and safety were retrospectively investigated in patients who underwent Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG) over a 6-year period in a single institution. (bvsalud.org)
Absence1
  • Because of its absence, reticulocytes are extremely difficult to process when it comes to why they are in such high or low numbers. (ihtbio.com)
Levels1
  • Those reticulocytes with particularly low levels of PK exhibit very low glycolytic rates and thus are uniquely reliant upon oxidative phosphorylation. (jci.org)
High2
  • This percentage is how they can determine whether you have high reticulocyte numbers. (ihtbio.com)
  • Yet when your reticulocyte numbers are not low but extremely high, different issues may be occurring within your body. (ihtbio.com)
Normal1
  • A custom bioinformatics analysis pipeline was developed to identify HBB variants within the scRNAseq data to identify individual reticulocytes expressing either normal HBB , sickle HBB , or both. (confex.com)
Mechanism1
  • Ochoa, S. (1977) Mechanism of translational control by hemin in reticulocyte lysates Proceedings of the National Academy of Sciences of the United States of America, 74 (8). (ias.ac.in)
Patients5
  • Cancer patients that are recovering from chemotherapy will usually have low reticulocyte numbers. (ihtbio.com)
  • résumé En dépit de l'idée communément admise selon laquelle les patients atteints de drépanocytose souffrent d'une surcharge en fer, certains d'entre eux sont atteints d'une anémie ferriprive. (who.int)
  • Nous avons examiné des patients yéménites âgés de 12 mois à 30 ans atteints de drépanocytose homozygote afin d'établir leur bilan en fer à l'aide de quatre critères (taux de fer sérique faible, taux de saturation de la transferrine faible, capacité totale de fixation du fer élevée et volume globulaire moyen faible par rapport à l'âge). (who.int)
  • L'objectif de notre étude est de décrire les caractéristiques hématologiques des patients atteints de COVID-19 hospitalisés au niveau du CHU d'Oran en Algérie. (bvsalud.org)
  • Patients et méthodes : Il s'agit d'une étude prospective menée entre le 1er Juillet et le 15 Octobre 2020 ayant inclus des patients hospitalisés et/ou suivis au niveau des unités COVID-19 du CHU Oran en Algérie. (bvsalud.org)