KB Cells: This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.Folate Receptors, GPI-Anchored: Cell surface receptors that bind to and transport FOLIC ACID, 5-methyltetrahydrofolate, and a variety of folic acid derivatives. The receptors are essential for normal NEURAL TUBE development and transport folic acid via receptor-mediated endocytosis.Mouth Neoplasms: Tumors or cancer of the MOUTH.Adenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.Carcinoma: A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)Cell Line: Established cell cultures that have the potential to propagate indefinitely.Arecoline: An alkaloid obtained from the betel nut (Areca catechu), fruit of a palm tree. It is an agonist at both muscarinic and nicotinic acetylcholine receptors. It is used in the form of various salts as a ganglionic stimulant, a parasympathomimetic, and a vermifuge, especially in veterinary practice. It has been used as a euphoriant in the Pacific Islands.Folic Acid: A member of the vitamin B family that stimulates the hematopoietic system. It is present in the liver and kidney and is found in mushrooms, spinach, yeast, green leaves, and grasses (POACEAE). Folic acid is used in the treatment and prevention of folate deficiencies and megaloblastic anemia.Drug Resistance: Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.Hypoxanthines: Purine bases related to hypoxanthine, an intermediate product of uric acid synthesis and a breakdown product of adenine catabolism.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Zingiberaceae: A plant family of the order Zingiberales, subclass Zingiberidae, class Liliopsida. It includes plants which have both flavoring and medicinal properties such as GINGER; turmeric (CURCUMA), and cardamom (ELETTARIA).Satellite Viruses: Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Areca: A plant genus of the family ARECACEAE. Members contain ARECOLINE and CATECHIN. The leaves and nuts have been used as masticatories, stimulants, and astringents in traditional medicine. The common name of betel is also used for PIPER BETLE. The common name of catechu is sometimes used for ACACIA CATECHU.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Deoxyribonucleases: Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.Receptors, Cell Surface: Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.Molecular Weight: The sum of the weight of all the atoms in a molecule.ThymidineTeniposide: A semisynthetic derivative of PODOPHYLLOTOXIN that exhibits antitumor activity. Teniposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent cells from entering into the mitotic phase of the cell cycle, and lead to cell death. Teniposide acts primarily in the G2 and S phases of the cycle.Dendrimers: Tree-like, highly branched, polymeric compounds. They grow three-dimensionally by the addition of shells of branched molecules to a central core. The overall globular shape and presence of cavities gives potential as drug carriers and CONTRAST AGENTS.Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)UridineTetrahydrofolates: Compounds based on 5,6,7,8-tetrahydrofolate.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Rats, Inbred BB: A strain of Rattus norvegicus which is a model for spontaneous insulin-dependent diabetes mellitus (DIABETES MELLITUS, INSULIN-DEPENDENT).Diabetes Mellitus, Type 1: A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.Euthanasia, Active: The act or practice of killing for reasons of mercy, i.e., in order to release a person or animal from incurable disease, intolerable suffering, or undignified death. (from Beauchamp and Walters, Contemporary Issues in Bioethics, 5th ed)Caseins: A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.ACTH-Secreting Pituitary Adenoma: A pituitary adenoma which secretes ADRENOCORTICOTROPIN, leading to CUSHING DISEASE.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Dialysis Solutions: Solutions prepared for exchange across a semipermeable membrane of solutes below a molecular size determined by the cutoff threshold of the membrane material.
  • Induction of autophagy by amino acid deprivation in bovine aortic endothelial cells caused an early and potent increase in the fluorescence of the proposed autophagy dye Cyto-ID. (springer.com)
  • Because we found that HUVECs and immortalized human dermal microvascular endothelial cells express small numbers of C3aRs and C5aRs, we asked what the function of these receptors was on these cells. (jimmunol.org)
  • C5a, but not C3a, was chemotactic for human immortalized dermal microvascular endothelial cells. (jimmunol.org)
  • The C5aR in endothelial cells thus uses a signaling cascade-transactivation of the EGFR-that does not exist in leukocytes, while the C3aR couples to a different G protein, presumably G α12/13 . (jimmunol.org)
  • Although it was reported that expression of C5aRs is low on endothelial cells ( 13 , 14 ), it is incorrect to conclude that activation of these receptors is functionally irrelevant, because engagement of a few hundred G protein-coupled receptors (GPCRs) 3 per cell is sufficient for maximal cell activation ( 15 ). (jimmunol.org)
  • Indeed, it has been suggested that endothelial cell-derived superoxide generation played a role in C5a-mediated pulmonary injury ( 16 ). (jimmunol.org)
  • Their expression has been described in the CNS ( 17 , 18 ), on epithelial cells ( 19 ), and on endothelial cells following ischemia/reperfusion ( 20 ). (jimmunol.org)
  • In this study, we report that cultured human endothelial cells express C3aRs and C5aRs, and that the two receptors use different signal transduction cascades to activate cellular responses distinct from those on leukocytes. (jimmunol.org)
  • To investigate the specific transcriptional effects of VEGFR-2 and VEGFR-3 activation, we performed a correlation analysis of previously published CAGE sequencing and microarray data of human lymphatic endothelial cells (LECs) stimulated with distinct VEGFs acting through either VEGFR-2 or VEGFR-3. (nature.com)
  • VEGFs exert their function by binding to the three known VEGFRs, VEGFR-1, VEGFR-2 or VEGFR-3, which are expressed by blood vessel endothelial cells (BECs) in case of VEGFR-1 and -2, and lymphatic vessel endothelial cells (LECs) in case of VEGFR-2 and -3. (nature.com)
  • The single-tube amplification (STA) system was applied to single to 100 cells of 293T cells, human pluripotent stem cells (hPSCs) and their differentiated endothelial progenies to validate its quantitative power and sensitivity by qPCR and high-throughput sequencing. (biomedcentral.com)
  • With 100 human embryonic stem cells (hESCs) and their differentiated endothelial cells as input for high-throughput sequencing, the single-tube amplification (STA) system revealed both well-known and other miRNAs selectively enriched in each cell type. (biomedcentral.com)
  • Leukocyte recruitment to the endothelium is started when inducible adhesion molecule receptors on the surface of endothelial cells interact with counterreceptors on immune cells. (google.es)
  • Established KS tumors consist predominantly of spindle-shaped cells of apparent endothelial origin which are regarded as the primary proliferative component of the lesion. (asm.org)
  • ERRα is widely expressed in normal tissues ( 2 ) and RNA expression studies show the presence of ERRα in a range of breast cancer cell lines ( 17 ). (aacrjournals.org)
  • NK cells are present in lymphoid organs as well as in nonlymphoid peripheral tissues ( 2 ). (pnas.org)
  • Bulk RNA sequencing (RNA-seq) of human tissues and cell lines has been applied to identify and quantify different splicing events [ 6 ], where in particular exon skipping at cassette exons, the most prevalent form of alternative splicing [ 1 ], has received considerable attention. (springer.com)
  • Pluripotent Stem- Cells Stem Cells which can develop into any of the three major tissue types: endoderm (interior gut lining), mesoderm (muscle, bone, blood), and ectoderm (epidermal tissues and nervous system). (counterbalance.org)
  • After allogeneic stem cell transplantation (alloSCT), severe graft-versus-host disease (GVHD) can be induced by donor T-cells recognizing normal tissues of the recipient. (frontiersin.org)
  • The course includes modules that emphasise structure and function in the cells of animals, plants, and microbes, in addition to how cells interact as they form tissues and embryos. (st-andrews.ac.uk)
  • Alternative HFE splicing variants may serve as iron regulatory mechanisms in specific cells or tissues. (wikipedia.org)
  • The recently discovered KS-associated herpesvirus (KSHV) ( 10 ), also known as human herpesvirus 8, is strongly linked to KS epidemiologically (see references 11 , 18 , and 45 for review) and is consistently detected in the spindle cells of KS tissues ( 5 , 48 , 49 ). (asm.org)
  • For the first time, we have performed detailed quantitative analyses of BC200 expression in a variety of human tissues, cancer cell lines and cultured primary cells that challenge the paradigm that BC200 is restricted to a neuronal and tumour expression pattern. (springermedizin.de)
  • 1977) "T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells," Proc. (freepatentsonline.com)
  • The ability of paclitaxel and another taxane, docetaxel ( Fig. 1 ), to kill tumor cells has made them useful chemotherapeutic agents against several types of cancers, including those derived from ovary, breast, head and neck, and lung as well as malignant melanoma ( 2 ). (aacrjournals.org)
  • In KS and PEL, the majority of the tumor cells display latent KSHV infection, while lytic replication is limited to a small subset of cells in both cases, presumably reflecting spontaneous reactivation from the latent state ( 5 , 48 , 49 ). (asm.org)
  • The receptor activator of nuclear factor-kB (NF-kB) (RANK)/receptor activator of NF-kB ligand (RANKL) axis emerges as a key regulator of breast cancer initiation, progression and metastasis. (biomedcentral.com)
  • The receptor activator of nuclear factor-kB (RANK), ligand (RANK-L) and osteoprotegerin (OPG) are implicated in the pathogenesis of acute Charcot neuroarthropathy (CN). (medsci.org)
  • Charcot neuroarthropathy, receptor activator of nuclear factor-kB, peripheral blood cells. (medsci.org)
  • Bergamini A, Bolacchi F, Pesce CD, Veneziano G, Uccioli L, Girardi V, De Corato L, Mondillo MT, Squillaci E. Expression of the receptor activator of nuclear factor-kB ligand in peripheral blood mononuclear cells in patients with acute Charcot neuroarthropathy. (medsci.org)
  • In vivo NK cell depletion has thus so far relied on anti-asialo-GM1 or anti-NK1.1 depleting antibodies. (pnas.org)
  • Senescent progenitor cell depletion as a potential therapy for hyposalivation in primary Sjogren's syndrome? (rug.nl)
  • T-cell depletion (TCD) of the stem cell graft is an effective strategy to prevent GVHD, and alloSCT regimens using the CD52 antibody alemtuzumab for TCD or using infusion of purified CD34 cells demonstrate efficient engraftment and reduced acute GVHD ( 1 - 4 ). (frontiersin.org)
  • Luciferase assays were employed to assess the contribution of each isoform alone or in combinations on NF-kB activation. (biomedcentral.com)
  • Wound healing and transwell assays were employed to evaluate the effect of TNFRSF11A isoforms on migration of MDA-MB-231 and 293T cells. (biomedcentral.com)
  • Results shown are representative of three assays with similar results. (springer.com)
  • Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. (perkinelmer.com)
  • FroZen, -irradiated cells, are a well established product, that can be ordered as a consumable, and readily used to perform Aequorin functional assays (AequoZen) or cAMP assays (cAMPZen). (perkinelmer.com)
  • Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. (perkinelmer.com)
  • Luminex Screening Assays are our most cost-effective and flexible kits for multianalyte profiling of cell culture supernatants, serum or plasma samples. (fishersci.com)
  • Our study revealed a new mechanism that PN inhibits TNF-a-mediated NF-kB activation via disrupting the recruitment of the IB kinases (IKK) complex to TNF receptor, which then blocked the subsequent signaling events including IKK kinase activation, IB degradation, p65 nuclear translocation, DNA binding and transactivation. (cdc.gov)
  • Existing methods for growing networks of neurons cannot produce such neat patterns and clean links between cells. (zdnet.com)
  • In the brain, it helps direct the movement (migration) of nerve cells (neurons) during early development. (medlineplus.gov)
  • In addition, RANK-c suppresses cell migration and represses the tumorigenic properties of invasive breast carcinoma cells. (biomedcentral.com)
  • Finally, isoform RANK-c seems to have the capacity to regulate signaling through wild type RANK and moreover to inhibit cell motility and migration of breast cancer cells. (biomedcentral.com)
  • The migration of oral squamous cell carcinoma in to maxillary and mandibular bones is a common clinical problem ( Sumitra, Nagani, 2013 ). (scielo.br)
  • 4. The method of claim 1, wherein the redox-sensitive gene is selected from the group consisting of those media the expression of expressing cytokines involved in initiating the immune response, chemoattractants that promote the migration of inflammatory cells to a point of injury, growth factors, and adhesion molecules. (google.es)
  • Briefly, peripheral blood was obtained from CLL patients, and mononuclear cells were isolated through Ficoll/Hypaque gradient centrifugation (Amersham Pharmacia Biotech) and then processed for RNA and DNA extraction according to standard protocols ( 14 ). (pnas.org)
  • This study aimed to investigate the expression of RANK-L and OPG in peripheral blood mononuclear cells (PBMC) from patients with acute CN. (medsci.org)
  • The purpose of this study was to determine the influence of mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on the surface properties of cells, such as wettability, contact angle, and surface heterogeneity. (arvojournals.org)
  • To additionally understand the significance of the MAD2 to mitotic checkpoint control, we established an inducible expression system in which MAD2 was induced by the addition of ponasterone A. Notably, the induced expression of MAD2 in two checkpoint-defective ovarian cancer cell lines led to the restoration of mitotic checkpoint response to spindle-disrupting agents. (aacrjournals.org)
  • Interferon regulatory factor (IRF) 3 activity in cell nuclear extracts was measured by a TransAM IRF3 kit. (arvojournals.org)
  • A rabbit TLR3 antibody, endosomal acidification inhibitor chloroquin, PKR inhibitor 2-aminopurine, IKK inhibitor II wedelolactone, or NF-kB activation inhibitor quinazoline blocked the activation of IkB- and NF-kB, inhibited the nuclear IRF3 activity, and further suppressed TSLP expression and production, partially or completely. (arvojournals.org)
  • The effects of asbestos (1332214) on pulmonary nuclear-factor-kappa- B (NF-kB) activity were studied in rats. (cdc.gov)
  • Monocytes, macrophages and dendritic cells (DCs) are the main targets for viruses involved in vascular permeability induction (Schnittler & Feldmann 2003, Clyde et al. (scielo.br)
  • HLA class II-expressing cells were characterized as activated T-cells, antigen-presenting cells (APCs), or tissue repairing macrophages. (frontiersin.org)
  • An increase in HLA class II-positive macrophages and CD3 T-cells was observed 12-24 weeks after myeloablative alloSCT, but these macrophages did not show signs of interaction with the co-localized T-cells. (frontiersin.org)
  • In contrast to the inflamed situation in GVHD, when interaction takes place between activated APC and donor T-cells, the tissue damage caused by myeloablative alloSCT results in dermal recruitment of HLA class II-positive tissue repairing macrophages co-existing with increased numbers of patient- and donor-derived T-cells, but without signs of specific interaction and initiation of an immune response. (frontiersin.org)
  • HFE is prominent in small intestinal absorptive cells, gastric epithelial cells, tissue macrophages, and blood monocytes and granulocytes, and the syncytiotrophoblast, an iron transport tissue in the placenta. (wikipedia.org)
  • The aim of this study was to investigate modulation of T cell immunity for the treatment of experimental arthritis, via enhanced expression of SOCS-3 in splenic antigen-presenting cells (APCs) obtained after intravenous injection of adenovirus encoding SOCS-3. (wiley.com)
  • Although treatments including chemotherapy and stem cell transplantation are developing very fast, patients still suffer from relapse and treatment related complications. (biomedcentral.com)
  • Here, we analyze the transcriptomes of human embryonic stem cell-derived lineage-specific progenitors by single-cell RNA-sequencing (scRNA-seq). (biomedcentral.com)
  • We report the analysis of 1776 cells by scRNA-seq covering distinct human embryonic stem cell-derived progenitor states. (biomedcentral.com)
  • All of our antibodies are guaranteed to perform in the stated application and species including areas such as cancer and stem cell research. (fishersci.com)
  • Conditioning regimens preceding allogeneic stem cell transplantation (alloSCT) can cause tissue damage and acceleration of the development of graft-versus-host disease (GVHD). (frontiersin.org)
  • There is a strong incentive for developing a pituitary stem cell therapy that could repopulate the pituitary hormone producing cells that are missing in patients with hypopituitarism. (elifesciences.org)
  • This model paves a way for the in vivo characterization and preclinical assessment of NK cell biological function. (pnas.org)
  • However, several NK cell features have limited our understanding of their biological function. (pnas.org)
  • We sought to determine whether the presence of natural autoreactive antibodies of B1a cell origin would play a role in the initiation of type 1 diabetes. (springer.com)
  • In NK1.1 − mouse strains, the identification of NK cells is based on the expression of the integrin subunit CD49b that is recognized by DX5 antibodies ( 12 ), despite its expression on T cell and myeloid subsets ( 13 , 14 ). (pnas.org)
  • isohaemagglutinin) n. one of the antibodies occurring naturally in the plasma that cause agglutination of red blood cells of a different group. (oxfordreference.com)
  • Although regulations of MUC1 and MUC4 mucin genes by bile acids, such as DCA, CDCA and TCA, in human oesophageal cancer cells have been the thorough extensive study [ 14 - 16 ], the mechanisms responsible for regulation of MUC2 expression in the esophageal adenocarcinoma cells remain unknown. (biomedcentral.com)
  • Cancer cells usually possess and tolerate higher ROS activity than normal cells [ 3 ]. (biomedcentral.com)
  • Despite the plethora of organs and cell types that depend on RANK function, little is known about the regulatory mechanisms that govern its functions both in normal cells and cancer cells. (biomedcentral.com)
  • Therapeutic strategies designed to target TP53-deficient cancer cells remain elusive. (jci.org)
  • It is thus believed that suppressed NF-kB activation and sustained JNK activation contribute to the sensitization effect of PN to TNF-a-mediated cell death in human cancer cells. (cdc.gov)
  • The antioxidative enzyme catalase is important to protect cancer cells against cytotoxic hydrogen peroxide. (biomedcentral.com)
  • Cells were cultured in serum-free medium (control) or in EBSS for 2 h, after which, labelling with Cyto-ID and MDC was carried out as detailed in Materials and methods. (springer.com)
  • working together to create and maintain a platform with open standards and tools, designed to enable secure storage, governance, analytics, access control and controlled sharing of information at multiple levels along with methods to make results of analyses and outcomes comparable across centers and sustainable through Rheumatology associations. (europa.eu)
  • Materials and Methods: In this experimental study, CD133+hematopoietic stem cells were isolated from umbilical cord blood and cultured in the presence of macrophage colony-stimulating factor (M-CSF) and RANKL. (sid.ir)
  • We identified that specific activation of VEGFR-3 by VEGF-C156S results in the downregulation of many genes involved in immune regulation and inflammation, suggesting that VEGFR-3 stimulation has direct anti-inflammatory effects. (nature.com)
  • Flavivirus-infected cultures exhibited dendritic cell activation and maturation molecules. (scielo.br)
  • In both situations, the RNAs are expressed at variable levels in latently infected cells and at much higher levels in cells undergoing viral reactivation ( 48 ). (asm.org)