PC12 Cells: A CELL LINE derived from a PHEOCHROMOCYTOMA of the rat ADRENAL MEDULLA. PC12 cells stop dividing and undergo terminal differentiation when treated with NERVE GROWTH FACTOR, making the line a useful model system for NERVE CELL differentiation.Proprotein Convertase 2: A serine endopeptidase that has specificity for cleavage at ARGININE. It cleaves a variety of prohormones including PRO-OPIOMELANOCORTIN, proluteinizing-hormone-releasing hormone, proenkephalins, prodynorphin, and PROINSULIN.Proprotein Convertase 1: A CALCIUM-dependent endopeptidase that has specificity for cleavage at ARGININE that is near paired basic residues. It cleaves a variety of prohormones including PRO-OPIOMELANOCORTIN; PRORENIN; proenkephalins; prodynorphin; prosomatostatin; and PROINSULIN.Proprotein Convertase 5: A serine endopeptidase found primarily in the EXTRACELLULAR MATRIX. It has specificity for cleavage of a variety of substrates including PRORENIN, pro-membrane type-1 matrix metalloproteinase, and NEURAL CELL ADHESION MOLECULE L1.Pheochromocytoma: A usually benign, well-encapsulated, lobular, vascular tumor of chromaffin tissue of the ADRENAL MEDULLA or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of EPINEPHRINE and NOREPINEPHRINE, is HYPERTENSION, which may be persistent or intermittent. During severe attacks, there may be HEADACHE; SWEATING, palpitation, apprehension, TREMOR; PALLOR or FLUSHING of the face, NAUSEA and VOMITING, pain in the CHEST and ABDOMEN, and paresthesias of the extremities. The incidence of malignancy is as low as 5% but the pathologic distinction between benign and malignant pheochromocytomas is not clear. (Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1298)Proprotein Convertases: Proteolytic enzymes that are involved in the conversion of protein precursors such as peptide prohormones into PEPTIDE HORMONES. Some are ENDOPEPTIDASES, some are EXOPEPTIDASES.Phosphatidylcholines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.Nerve Growth Factor: NERVE GROWTH FACTOR is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth stimulating activity.Neurites: In tissue culture, hairlike projections of neurons stimulated by growth factors and other molecules. These projections may go on to form a branched tree of dendrites or a single axon or they may be reabsorbed at a later stage of development. "Neurite" may refer to any filamentous or pointed outgrowth of an embryonal or tissue-culture neural cell.Adrenal Gland Neoplasms: Tumors or cancer of the ADRENAL GLANDS.Furin: A proprotein convertase with specificity for the proproteins of PROALBUMIN; COMPLEMENT 3C; and VON WILLEBRAND FACTOR. It has specificity for cleavage near paired ARGININE residues that are separated by two amino acids.Neuroendocrine Secretory Protein 7B2: An acidic protein found in the NEUROENDOCRINE SYSTEM that functions as a molecular chaperone for PROPROTEIN CONVERTASE 2.Prostatic Neoplasms: Tumors or cancer of the PROSTATE.Nerve Growth Factors: Factors which enhance the growth potentialities of sensory and sympathetic nerve cells.Laminin: Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.Receptors, Nerve Growth Factor: Cell surface receptors that bind NERVE GROWTH FACTOR; (NGF) and a NGF-related family of neurotrophic factors that includes neurotrophins, BRAIN-DERIVED NEUROTROPHIC FACTOR and CILIARY NEUROTROPHIC FACTOR.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Helper Viruses: Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Cell Line: Established cell cultures that have the potential to propagate indefinitely.Murine Acquired Immunodeficiency Syndrome: Acquired defect of cellular immunity that occurs in mice infected with mouse leukemia viruses (MuLV). The syndrome shows striking similarities with human AIDS and is characterized by lymphadenopathy, profound immunosuppression, enhanced susceptibility to opportunistic infections, and B-cell lymphomas.Leukemia Virus, Murine: Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Vesicular Monoamine Transport Proteins: A family of vesicular amine transporter proteins that catalyze the transport and storage of CATECHOLAMINES and indolamines into SECRETORY VESICLES.Tetrabenazine: A drug formerly used as an antipsychotic and treatment of various movement disorders. Tetrabenazine blocks neurotransmitter uptake into adrenergic storage vesicles and has been used as a high affinity label for the vesicle transport system.Vesicular Biogenic Amine Transport Proteins: Integral membrane proteins of the LIPID BILAYER of SECRETORY VESICLES that catalyze transport and storage of biogenic amine NEUROTRANSMITTERS such as ACETYLCHOLINE; SEROTONIN; MELATONIN; HISTAMINE; and CATECHOLAMINES. The transporters exchange vesicular protons for cytoplasmic neurotransmitters.Chromaffin Granules: Organelles in CHROMAFFIN CELLS located in the adrenal glands and various other organs. These granules are the site of the synthesis, storage, metabolism, and secretion of EPINEPHRINE and NOREPINEPHRINE.Adrenal Medulla: The inner portion of the adrenal gland. Derived from ECTODERM, adrenal medulla consists mainly of CHROMAFFIN CELLS that produces and stores a number of NEUROTRANSMITTERS, mainly adrenaline (EPINEPHRINE) and NOREPINEPHRINE. The activity of the adrenal medulla is regulated by the SYMPATHETIC NERVOUS SYSTEM.Reserpine: An alkaloid found in the roots of Rauwolfia serpentina and R. vomitoria. Reserpine inhibits the uptake of norepinephrine into storage vesicles resulting in depletion of catecholamines and serotonin from central and peripheral axon terminals. It has been used as an antihypertensive and an antipsychotic as well as a research tool, but its adverse effects limit its clinical use.Cytoplasmic Granules: Condensed areas of cellular material that may be bounded by a membrane.Organotin Compounds: Organic compounds which contain tin in the molecule. Used widely in industry and agriculture.Trimethyltin Compounds: Organic compounds composed of tin and three methyl groups. Affect mitochondrial metabolism and inhibit oxidative phosphorylation by acting directly on the energy conserving processes.Trialkyltin Compounds: Organometallic compounds which contain tin and three alkyl groups.Triethyltin Compounds: Organic compounds composed of tin and three ethyl groups. Affect mitochondrial metabolism and inhibit oxidative phosphorylation by acting directly on the energy conserving processes.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Tin: A trace element that is required in bone formation. It has the atomic symbol Sn, atomic number 50, and atomic weight 118.71.Adenomatous Polyposis Coli Protein: A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.beta Catenin: A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.alpha Catenin: A catenin that binds F-ACTIN and links the CYTOSKELETON with BETA CATENIN and GAMMA CATENIN.Adenomatous Polyposis Coli: A polyposis syndrome due to an autosomal dominant mutation of the APC genes (GENES, APC) on CHROMOSOME 5. The syndrome is characterized by the development of hundreds of ADENOMATOUS POLYPS in the COLON and RECTUM of affected individuals by early adulthood.Catenins: A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking CADHERINS to the ACTIN FILAMENTS of the CYTOSKELETON.Stem Cell Research: Experimentation on STEM CELLS and on the use of stem cells.Stilbenes: Organic compounds that contain 1,2-diphenylethylene as a functional group.Heme Oxygenase-1: A ubiquitous stress-responsive enzyme that catalyzes the oxidative cleavage of HEME to yield IRON; CARBON MONOXIDE; and BILIVERDIN.Aryldialkylphosphatase: An enzyme which catalyzes the hydrolysis of an aryl-dialkyl phosphate to form dialkyl phosphate and an aryl alcohol. It can hydrolyze a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters. It may also mediate an enzymatic protection of LOW DENSITY LIPOPROTEINS against oxidative modification and the consequent series of events leading to ATHEROMA formation. The enzyme was previously regarded to be identical with Arylesterase (EC 3.1.1.2).Heme: The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.Heme Oxygenase (Decyclizing): A mixed function oxidase enzyme which during hemoglobin catabolism catalyzes the degradation of heme to ferrous iron, carbon monoxide and biliverdin in the presence of molecular oxygen and reduced NADPH. The enzyme is induced by metals, particularly cobalt. EC 1.14.99.3.Antioxidants: Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.Longevity: The normal length of time of an organism's life.Phosphate-Binding Proteins: Proteins that bind to and are involved in the metabolism of phosphate ions.Synaptic Vesicles: Membrane-bound compartments which contain transmitter molecules. Synaptic vesicles are concentrated at presynaptic terminals. They actively sequester transmitter molecules from the cytoplasm. In at least some synapses, transmitter release occurs by fusion of these vesicles with the presynaptic membrane, followed by exocytosis of their contents.Sodium-Phosphate Cotransporter Proteins, Type III: A family of highly conserved and widely expressed sodium-phosphate cotransporter proteins. They are electrogenic sodium-dependent transporters of phosphate that were originally identified as retroviral receptors in HUMANS and have been described in yeast and many other organisms.Biological Transport, Active: The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Phosphates: Inorganic salts of phosphoric acid.Exocytosis: Cellular release of material within membrane-limited vesicles by fusion of the vesicles with the CELL MEMBRANE.
PC12 cells were used to find which prion protein fragments caused neuronal dysfunction. PC12 was used partly for some forms of ... This makes PC12 cells useful as a model system for neuronal differentiation and neurosecretion. Treatment of PC12 cells with ... been studied using this cell line to understand their effects on PC12 cells These types of studies show that use of PC12 cell ... "PC12 cells" [1] Ras function was partially elucidated using PC12 cell line [2], from section in Cancer Medicine textbook ...
PC12) cell line. In this study, we assayed the cell viability, the percentage of lactate dehydrogenase released (% LDH released ... health and quality of life may be partly explained by a deficit in cysteine and (ii) that the dietary consumption of cysteine ... The spleen cells immune response to sheep red blood cells of C3H/HeJ mice fed a 20 g whey protein/100 g diet is substantially ... The plaque-forming cell response to sheep red blood cells was found to be enhanced in mice fed a formula diet containing 20 g ...
... inside the channel pore to block the entry of positive ions to the cell therefore inhibiting cell depolarisation.[63][64] ... PC12) (Ki = 136 nM), and the serotonin transporter (Ki = 2,234 nM).[41] The study notably found Ki values of ,10,000 nM for the ... PCP's rewarding and reinforcing effects are at least partly mediated by blocking the NMDA receptors in the glutamatergic inputs ... normally the receptor acts as an ion channel and there is an influx of positive ions through the channel to cause nerve cell ...
4). Furthermore, we minimized the effect of stabilized β-catenin on cell-cell adhesion by counting only PC12 cells that had not ... 3A, solid arrowhead) or in a part of the growth cone just proximal to and partly overlapping with enriched APC (Fig. 3A, hollow ... PC12 cells were passaged and treated with NGF for 3.5 days. Cells were stained for tubulin and ,250 cells were counted for the ... in the well-studied PC12 pheochromocytoma cell line (Greene et al., 1998). PC12 cells induced with NGF are a good model for APC ...
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... WISP1 was mainly located in cytoplasm of PC12 or SHSY5Y cells. Compared with the negative control group, WISP1 expression ... and WISP1 colocalized with NeuN partly. WISP1 colocalized with LC3, but there was little of colocation about WISP1 with cleaved ...
1990) Nerve Growth Factor Induces Increased Expression of a Laminin-Binding Integrin in Rat Pheochromocytoma PC12 Cells. ... TITLE: Expression of PKC Iota Affects Neuronal Differentiation of PC12 Cells at Least Partly Independent of Kinase Function ... in the early neuronal differentiation of PC12 cells, was investigated. NGF-treated PC12 cells with stably expressed exogenous ... Overall, these findings suggest that in PC12 and perhaps other neuroendocrine precursor cells, PKCι influences an early ...
TITLE: Expression of PKC Iota Affects Neuronal Differentiation of PC12 Cells at Least Partly Independent of Kinase Function ... in the early neuronal differentiation of PC12 cells, was investigated. NGF-treated PC12 cells with stably expressed exogenous ... Overall, these findings suggest that in PC12 and perhaps other neuroendocrine precursor cells, PKCι influences an early ... Pharmacological inhibition of endogenous aPKC kinase activity in parental PC12 cells did not inhibit neurite formation, ...
When PC12 cells were transiently transfected with plasmids having a luciferase reporter gene under the control of the TH ... SCFA are capable of altering host gene expression, partly due to their histone deacetylase inhibitor activity. We have ... mRNA levels in a PC12 cell model. Since monoamine concentration is known to be elevated in the brain and blood of ASD patients ... At lower concentrations PPA also caused accumulation of TH mRNA and protein, indicative of increased cell capacity to produce ...
These findings indicate that a direct effect of fasudil mesylate on PC12 cells may be partly responsible for its protective ... In this study, the properties of FM on hydrogen peroxide (H(2)O(2))-induced oxidative stress insult in cultured PC12 cells as ... FM protected PC12 cells from oxidative stress insult via downregulating the Bax/Bcl-2 ratio. ... Pretreatment with FM (5, 10 μM) prior to H(2)O(2) exposure significantly elevated cell viability, reduced cell apoptosis by MTT ...
To rule out the possibility that the reserpine-induced elevation of GSH levels was (partly) attributable to enhanced DA ... Effect of reserpine on dopamine (DA) content (A) and glutathione (GSH) content (B) of PC12 cells. PC12 cells, cultured for 2 d ... Effect of reserpine on glutathione (GSH) content of PC12 cells in the presence of cystine. PC12 cells, cultured for 2 d, were ... Cell culture. PC12 cells, originally described by Greene and Tischler (1976), were grown in 80 cm2 tissue culture flasks ...
PC12 cells were used to find which prion protein fragments caused neuronal dysfunction. PC12 was used partly for some forms of ... This makes PC12 cells useful as a model system for neuronal differentiation and neurosecretion. Treatment of PC12 cells with ... been studied using this cell line to understand their effects on PC12 cells These types of studies show that use of PC12 cell ... "PC12 cells" [1] Ras function was partially elucidated using PC12 cell line [2], from section in Cancer Medicine textbook ...
Knocking down PHB2 by siRNA partly increased the apoptosis level of PC12 stimulated by H2O2. While the PHB2 was interrupted by ... To further investigate the function of PHB2, primary cultured astrocytes and the neuronal cell line PC12 were employed to ... establish a proliferation model and an apoptosis model, respectively, to simulate the cell activity after TBI to a certain ... Recent studies have identified PHB2 as a multifunctional protein that controls cell proliferation, apoptosis, cristae ...
Stannin was expressed in several TMT-sensitive cell lines (PC12, T, B cells) and in primary neurons in culture. Stannin was ... Thus, TMT may induce apoptosis in sensitive cells, which is partly mediated by stannin. Based on the available data we conclude ... with sensitive cell lines (neuroblastomas, T-, B-cell lines) showing TC50 values of 2 to 8 microM, whereas insensitive cells ( ... NIH-3T3 fibroblast, HTB-14 glioma, TC-7 kidney cells) had TC 50 values , 100 microM. Primary neuronal cell cultures were very ...
... cells and sympathetic neurons, nerve growth factor (NGF) engagement with the tropomyosin-related tyrosine kinase (TrkA) ... In pheochromocytoma 12 (PC12) cells and sympathetic neurons, nerve growth factor (NGF) engagement with the tropomyosin-related ... Thus, NGF drives increased neurite growth rates partly by coupling the MAPK pathway to the activation of GSK3beta and thereby ... We now show, using pharmacological inhibitor studies of PC12 cells and sympathetic neurons in culture and in vitro kinase and ...
... have been reported in many cell types, but little is known about its direct effects on vesicular... ... at least partly) for the E2-induced inhibition of depolarization-evoked exocytosis. In cells that do not exhibit E2-stimulated ... Westerink RH, Ewing AG (2008) The PC12 cell as model for neurosecretion. Acta Physiol 192:273-285CrossRefGoogle Scholar ... Bennett DL, Bootman MD, Berridge MJ, Cheek TR (1998) Ca2+ entry into PC12 cells initiated by ryanodine receptors or inositol 1, ...
Furthermore, FLG and FLA treatments decreased the viability of the colon adenoma cell line DLD-1 and breast cancer cell line ... FLG and FLA attenuated the levels of intracellular oxidative stress in neuron-like PC-12 cells. The treatment of RAW 264.7 ... Moreover, the treatment with FLG or FLA combined with paclitaxel had synergistic anticancer effects on the DLD-1 cell line. (4 ... and anticancer effects of flavonol glycosides and aglycones from green tea using in vitro cell models. The fractions rich in ...
Resveratrol upregulates heme oxygenase-1 expression via activation of NF-E2-related factor 2 in PC12 cells. Biochem Biophys Res ... HUH7 cells were seeded at a density of 0.2 × 106 cells/well (for HUH7) or 0.15 × 106 cells/well (for HUH7/PON1) for 24 h and ... HUH7 cells were seeded in a 24-well-plate at a density of 0.1 × 106 cells for 24 h. Then cells were transfected with pARE_GIGPx ... HUH7 cells were seeded at a density of 1.0 × 106 cells/well in a 6 well plate for 24 h. Subsequently, cells were treated with ...
Immunoprecipitation as well as in vitro binding revealed that stathmin associated with endogenous Stat3 in PC12 cells. Nuclear ... whereas stathmin knockdown with siRNA increased tubulin polymerization and partly restored migration in MEFs lacking Stat3. ... Thus, Stat3 appears to regulate microtubule dynamics and cell migration through interactions with stathmin in the cell ... not only regulates cell proliferation and survival but also has been implicated in cell migration. However, no target genes ...
... the effects of various hydrolysates on H2O2-induced injury PC12 cells, and cognition of mice were investigated, respectively. ... The test of animals cognition indicated that three hydrolysates could present partly better effect of improving recurred ... Moreover, the hydrolysates also could reduce the rate of nonviable apoptotic cells at the concentration of 2 mg/mL. ...
BNPI expression confers saturable glutamate uptake in PC12 cell membranes. (A) Membranes prepared from transfected PC12 cells ( ... Evans blue (EB) (5 μM) also inhibits BNPI activity dramatically, whereas 1 μM DIDS blocks transport only partly, and 10 mM Pi ... Uptake by membranes from untransfected PC12 cells (also for 5 min) was subtracted from that observed in the transfected cell ... The rat BNPI cDNA was stably expressed in PC12 cells, and immunofluorescence for BNPI was used to identify the most highly ...
Effect of taurine on caspase-3 activity in HD-exposed PC12 cells. Data were presented as mean±SD, n=6. *p,0.05, compared with ... fig_006: Effect of taurine on caspase-3 activity in HD-exposed PC12 cells. Data were presented as mean±SD, n=6. *p,0.05, ... These results indicate that pretreatment of taurine may, at least partly, prevent HD-induced apoptosis via inhibiting ... fig_006: Effect of taurine on caspase-3 activity in HD-exposed PC12 cells. Data were presented as mean±SD, n=6. *p,0.05, ...
... is an important regulator of cell migration and plays a role in the scarring response in injured brain. It is also reported ... Song Y, Wei EQ, Zhang WP, Ge QF, Liu JR, Wang ML, Huang XJ, Hu X, Chen Z: Minocycline protects PC12 cells against NMDA-induced ... Activation of 5-lipoxygenase after oxygen-glucose deprivation is partly mediated via NMDA receptor in rat cortical neurons. J ... dendritic cells [32], monocyte-derived dendritic cells [33], vascular smooth muscle cells [34], intestinal epithelial cells [35 ...
Salidroside protects PC12 cells from HO-induced apoptosis at least partly by suppressing NOX2-ROS-MAPKs signaling pathway.,/p, ... SMMC-7721 cells were pretreated with different doses of ampelopsin and cells proliferation was detected by CCK8 kit. Cell ... Salidroside protects PC12 cells from HO-induced apoptosis via suppressing NOX2-ROS-MAPKs signaling pathway / 南方医科大学学报 ... p,,b,OBJECTIVE,/b,To investigate the molecular mechanism by which salidroside protects PC12 cells from HO-induced apoptosis.,/p ...
RhoE induces stress fiber disassembly in a variety of cell types, whereas RhoA stimulates stress fiber assembly. The similarity ... RhoE Stimulates Neurite-Like Outgrowth in PC12 Cells Through Inhibition of the RhoA/ROCK-I Signalling R Talens-Visconti et al. ... The Rho GTPase family member RhoE regulates actin filaments partly by binding to and inhibiting ROCK I, a serine/threonine ... Cells were lysed after 24 h, and cell lysates were incubated with GST, GST-RhoE, or GST-RhoA-V14 on glutathione beads. The ...
... in vitro studies suggest that α7 nicotinic receptor modulation is protective against NMDA-mediated excitotoxicity to PC12 cells ... 5, A and B). Thus, the attenuation by lobeline of the METH-induced decreases in VMAT-2 is partly temperature-dependent and is ... In conclusion, METH produces a decrease in VMAT-2 protein that is partly but not entirely mediated by hyperthermia. In addition ... Collectively, the protective effects of lobeline against depletions of dopamine and 5HT content after METH are due partly to ...
Cell culture and treatment. PC12 (TCR 9) cells were obtained from the Cell Bank of Shanghai Institute of Cell Biology, Chinese ... This is accompanied by hippocampal neuronal loss partly as a result of apoptosis, as evidenced by caspase-3 activation and PARP ... The PC12 cells (3 × 104 cells/well) were seeded on coverslips in polylysine-coated 24-well plate. After treatment, PC12 cells ... PBDE-47-induced autophagy alteration is more sensitive than apoptosis induction in PC12 cells. PC12 cells were exposed to 1.0, ...
Co-incubation of Akt inhibitor or p38 inhibitor partly attenuated the protection of SMXZF against H2O2-injured PC12 cells. In ... induced injury in PC12 cells, focusing on the Akt and MAPK pathways . The PC12 cells were exposed to H2O2 (400 μmol·L(-1)) for ... Cell Line, Tumor , Cell Survival , Drugs, Chinese Herbal , Chemistry , Toxicity , Glycosides , Chemistry , Toxicity , Humans , ... A combination of four effective components derived from Sheng-mai san attenuates hydrogen peroxide-induced injury in PC12 cells ...
decreased cell viability in both a dose- and time-dependent manner in PC12 cells. $CoCl_2$. -induced cell death produced ... into the cytoplasmic calcium store, which are at least partly relevant to the direct interaction with the nicotinic receptor ... cell viability loss in PC12 cells with a maximum inhibition at $0.5{\sim}1{\mu}M$. ; beyond these concentrations the inhibitory ... induce apoptosis in PC12 cells through both mitochondria- and death receptor-mediated cell death pathway. Estradiol was found ...
  • Bennett DL, Bootman MD, Berridge MJ, Cheek TR (1998) Ca 2+ entry into PC12 cells initiated by ryanodine receptors or inositol 1,4,5-trisphosphate receptors. (springer.com)
  • For example, Ins(1,4,5)P 3 -sensitive Ca 2+ stores, usually linked to a variety of G-protein-coupled cell surface receptors, are ubiquitous in mammalian cells, 1 2 4 but ryanodine-sensitive stores are not widely expressed among nonmuscle cells 4 5 (for exceptions, see Wu et al. (arvojournals.org)
  • The main objective of our study was to examine whether genetic deletion or pharmacological blockade of P2X 7 receptors influenced dopaminergic cell death in various models of Parkinson's disease (PD). (biomedcentral.com)
  • To test a requirement for Rho family members in cytoskeletal events mediated by structurally diverse receptors in leukocytes, we transfected the full-length human chemotactic peptide receptor in RAW 264.7 cells and examined cytoskeletal alterations in response to the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP), colony stimulating factor-1 (CSF-1), IgG-coated particles, and phorbol 12-myristate 13-acetate (PMA). (rupress.org)
  • The neurotoxicity is mainly due to N-methyl-D-aspartate (NMDA) receptors, which cause excessive elevation of intracellular Ca 2+ concentration ([Ca 2+ ] i ) and subsequent neuronal cell death [ 10 ]. (biomedcentral.com)
  • Endothelin (ET)-1 is produced in ovarian carcinoma cells and is known to act through ET A receptors as an autocrine growth factor in vitro and in vivo . (aacrjournals.org)
  • The latter effects include ET-1-driven positive feedback loops that are mediated by ET A receptors in cultured human ovarian (13 , 14) and prostate (15 , 16) carcinoma cells, and may contribute to tumor cell growth in vivo . (aacrjournals.org)
  • Accordingly, different studies have reported alterations of the expression and the function of Ryanodine Receptors (RyR) in human AD-affected brains, in cells expressing familial AD-linked mutations on the β amyloid precursor protein (βAPP) and presenilins (the catalytic core in γ-secretase complexes cleaving the βAPP, thereby generating amyloid β (Aβ) peptides), as well as in the brain of various transgenic AD mice models. (biomedcentral.com)
  • Recently, some studies showed that dopamine receptors could regulate several ways of death of the tumor cell, including apoptosis, autophagy-induced death, and ferroptosis, which cannot only directly affect tumor behavior, but also limit tumor progress via activating tumor immunity. (jcancer.org)
  • Moreover, the two receptors can be recruited to the cell surface by inflammatory cytokines, thereby increasing vulnerability to proNGF after brain injury ( Choi and Friedman, 2014 ). (eneuro.org)
  • We show that depletion of endogenous DA stores with reserpine was accompanied in PC12 cells by a long-lasting, significant increase in GSH content the extent of which appeared to be inversely related to the rate of GSH synthesis. (jneurosci.org)
  • The interaction of RhoE with ROCK I was confirmed by coimmunoprecipitation of the endogenous proteins, and the two proteins colocalized on the trans-Golgi network in COS-7 cells. (nih.gov)
  • A synthetic, cell-permeable homodimerizing reagent, AP20187, which has a greatly reduced affinity for endogenous FKBP12 (~250 nM), binds with high affinity (~0.1 nM) to a F36V mutant (Fv) form of FKBP12 (32). (sri.com)
  • Expression of a Munc18-1 deletion mutant lacking 17 residues of the domain 3a loop (Munc18-1 Δ317-333 ) in PC12 cells deficient in endogenous Munc18 (DKD-PC12 cells) fully rescued transport of Sx1a to the plasma membrane, but not exocytic secretory granule fusion. (biologists.org)
  • In addition, in a cell culture model, irisin restored ox-LDL-induced human umbilical vein endothelial cell dysfunction by reducing the levels of inflammatory genes via inhibiting the reactive oxygen species (ROS)/ p38 MAPK/ NF-κB signaling pathway activation and inhibiting cell apoptosis via up-regulating Bcl-2 and down-regulating Bax and caspase-3 expression. (plos.org)
  • This study demonstrates the presence of Ca 2+ stores in lysosomes of bovine corneal endothelial cells (BCECs) and examines their interaction with Ins(1,4,5)P 3 -sensitive Ca 2+ stores. (arvojournals.org)
  • In a study, pycnogenol prevented vascular endothelial cells from injury induced by an organic oxidant t-butyl hydroperoxide and inhibited macrophage oxidative burst, plasma low density lipoprotein (LDL) oxidation, and hydroxyl radical-induced plasmid DNA damage. (zhion.com)
  • Wylezinski LS & Hawiger J Interleukin 2 Activates Brain Microvascular Endothelial Cells Resulting in Destabilization of Adherens Junctions. (abcam.com)
  • Human microvascular HMEC-1 endothelial cells were incubated in low and high glucose concentrations (5 and 30 mmol/l, respectively), and activation of nrf2 was assessed by nuclear translocation. (termsreign.tk)
  • In contrast to the motor neurons, the cells from the other metrizamide fraction (including neuronal cells) could be kept in culture for a prolonged time without addition of exogenous factor(s). (uni-wuerzburg.de)
  • Accordingly, we propose a novel biological principle of cell-to-cell interaction based on membrane continuity and intercellular transfer of organelles. (sciencemag.org)
  • However, serial sectioning showed that, at any given point along TNTs, their membrane appeared to be continuous with the membranes of connected cells ( Fig. 1G ). (sciencemag.org)
  • Whereas the nascent phagosome is formed to a large extent by invagination of the plasma membrane ( 3 ), the membrane surface required to internalize multiple or large targets may represent an area equivalent to the entire cell surface ( 4 ). (jimmunol.org)
  • Cell membrane permeability was also altered to different extents in the presence of various ILs. (biomedcentral.com)
  • It also binds to some extent to the bacterial 50S ribosomal subunit and may alter the cytoplasmic membrane causing intracellular components to leak from bacterial cells. (pianolarge.gq)
  • Thus, TMT may induce apoptosis in sensitive cells, which is partly mediated by stannin. (aspetjournals.org)
  • The underlying mechanisms by which resveratrol may mediate beneficial effects have yet not been fully understood and may be partly related to its ability to induce phase II and antioxidant enzymes including heme oxygenase-1 and paraoxonase-1. (biomedcentral.com)
  • A lower degree of S/T phosphorylation of the Y654F mutant compared to Y86F in HEK293T cells correlated with a reduced amount of Axin detectable in Y654F immunoprecipitates, thus suggesting that the Bcr Abl mediated phosphorylation of Y86 could induce a conformational change of b catenin impairing its binding to Axin. (fakinhibitors.com)
  • Interestingly, cell survival signaling pathways are closely linked to those that end up in apoptosis. (biomedcentral.com)
  • In addition to the acute effects, the chronic state of β-cell secretory performance has been correlated to the activity of metabolic pathways that are instrumental in either NADPH generation, such as citrate cataplerosis ( 15 , 16 ) and pyruvate cycling ( 17 ), or glycolysis coupled to mitochondrial NADH shuttle systems ( 18 , 19 ). (diabetesjournals.org)
  • These findings indicate that ET-1-induced stimulation of Erk 2 phosphorylation, and mitogenic responses in OVCA 433 ovarian cancer cells are mediated in part by signaling pathways that are initiated by transactivation of the EGF-R. (aacrjournals.org)
  • These genetics are included in cell routine, eMT and angiogenesis pathways. (healthweblognews.info)
  • These isoprenoids modify recently translated proteins such as small GTPase molecules that are essential in numerous cell-signaling pathways, including vesicular trafficking and inflammation. (iospress.com)
  • Knocking down PHB2 by siRNA partly increased the apoptosis level of PC12 stimulated by H 2 O 2 . (mdpi.com)
  • These CSCs are included in cell development, migration/intrusion, and apoptosis level of resistance, attributing to treatment metastasis and level of resistance leading GW 501516 to a poor medical outcome3. (healthweblognews.info)
  • Overall, these findings suggest that in PC12 and perhaps other neuroendocrine precursor cells, PKCι influences an early differentiation decision between the neuroendocrine (chromaffin) and sympathetic neuron cell lineages, potentially by affecting PKCζ function. (scirp.org)
  • The vesicles hold catecholamines, mostly dopamine but also limited amount of norepinephrine, and release of these neurotransmitters give rise to spikes due to changes in current similar to chromaffin cells. (wikipedia.org)
  • TH is expressed in specific neurons of the central nervous system and in the periphery in sympathetic ganglia and adrenal medullary chromaffin cells. (aspetjournals.org)
  • We speculated that nifedipine might inhibit the TfR1/ DMT1 (transferrin receptor 1/divalent metal transporter1)-mediated iron uptake by proximal tubule cells in addition to blocking L-type Ca2+ channels, leading to an increase in iron in lumen-fluid and then urinary iron excretion. (frontiersin.org)
  • Akt regulates progesterone receptor B-dependent transcription and angiogenesis in endometrial cancer cells. (abcam.com)
  • In OVCA 433 human ovarian carcinoma cells, ET-1 caused phosphorylation of the epidermal growth factor receptor (EGF-R) that was accompanied by phosphorylation of Shc and its recruitment complexed with Grb2. (aacrjournals.org)
  • In this study, we investigated whether the ability of Eph receptor signaling to mediate cell repulsion is antagonized by fibroblast growth factor receptor (FGFR) activation that can promote cell invasion. (rupress.org)
  • Thus, the cell response appears to depend on the degree of receptor activation. (rupress.org)
  • Several lines of evidence raise the possibility that there is antagonism between the Eph-ephrin system and other receptor tyrosine kinases in the control of cell migration. (rupress.org)
  • Together with the observation that flotillin polarization does not occur in other polarized cell types such as polarized epithelial cells, our results suggest a specific role for flotillins in hematopoietic cell polarization. (genes2cognition.org)
  • Moreover, HD resulted in an increase in reactive oxygen species (ROS) level and a decline in the activities of superoxidedismutase and catalase in PC12 cells. (nih.gov)
  • The work supports the idea that soluble huntingtin, not aggregates, is responsible for cell death, and could lead to a new target for reducing the protein's toxicity. (alzforum.org)
  • Lowering of PC12 GSH content, via blockade of its synthesis with buthionine sulfoximine, however, led to a significantly decreased accumulation of exogenous [ 3 H]DA without affecting uptake of the acetylcholine precursor [ 14 C]choline. (jneurosci.org)
  • As expected, compared with control group, a significant increase in caspase-3 activity was observed in HD-intoxicated PC12 cells, which was significantly repressed once HD-intoxicated cells were pretreated with taurine, suggesting that HD-induced caspase-3 activation was prevented by taurine.Fig. 6. (nih.gov)
  • Our study demonstrated that irisin significantly reduced atherosclerosis in apolipoprotein E-deficient mice via suppressing ox-LDL-induced cell inflammation and apoptosis, which might have a direct therapeutic effect on atherosclerotic diseases. (plos.org)
  • We demonstrated for the first time that nifedipine significantly enhanced iron content as well as TfR1 and DMT1 expression and had no effect on Fpn1 levels in the cells. (frontiersin.org)
  • They found that DNA fragmentation was significantly higher in MCF-7 cells treated with pycnogenol than the untreated cells while pycnogenol did not significantly alter the number of apoptotic cells in MCF-10 samples. (zhion.com)
  • In addition, pretreatment with GSPE (6 μg/ml) for 5 min inhibited 0.1 mM [Mg 2+ ] o - and glutamate-induced formation of NO. Treatment with GSPE (6 μg/ml) significantly inhibited 0.1 mM [Mg 2+ ] o - and oxygen glucose deprivation-induced neuronal cell death. (biomedcentral.com)
  • In accord with this finding, the mitogenic action of ET-1 in OVCA 433 cells was also significantly reduced by a concentration of tyrphostin AG1478 that abolished the growth response of EGF-stimulated cells. (aacrjournals.org)
  • 2) Autophagosomes of A549 cells induced by Rh-endostatin and Erlotinib after monotherapy and combined treatment, the amount of autophagosomes was significantly increased compared with the control group, and the amount of autophagosomes in the combined group was more than the mono-drug treated groups. (dissertationtopic.net)
  • Erlotinib blocked cells at G0/G1 phase while induced significantly decreased G2/M phase. (dissertationtopic.net)
  • In NIH 3T3 cells, mutation of the c-FOS DEF domain significantly reduced AP-1 activity and inhibited the transforming activity associated with wild-type c-FOS [ 13 ], suggesting that AP-1 transcription factor contains a DEF domain like c-FOS and that sustained ERK signals largely contribute to the regulation of cellular phenotypes. (beds.ac.uk)
  • SCFA are capable of altering host gene expression, partly due to their histone deacetylase inhibitor activity. (plos.org)
  • Interestingly, blockage of apoptosis by caspase-3 inhibitor Ac-DEVD-CHO ameliorated PBDE-47-exerted autophagy impairment and cell death, though in combination with autophagy inhibitor did not further promote cell survival. (thno.org)
  • Cell permeable inhibitor of calpain I (Ki = 120 nM), calpain II (Ki = 230 nM), cathepsin B (Ki = 100 nM), and cathepsin L (Ki = 600 pM). (sri.com)
  • The weak inhibitory effect of SU6656, a pan Src family kinase inhibitor, on b catenin Y phosphorylation cannot exclude a minor contribution of other Src related kinases, which are also activated by Bcr Abl in CML cells. (fakinhibitors.com)
  • CEP-1347 (KT7515), an inhibitor of JNK activation, rescues sympathetic neurons and neuro- nally differentiated PC12 cells from downfall evoked nearby three distinct insults. (vet-md.com)
  • Anti-miR-219a-2-3p-IGF-Exo group: PC12 cells pretreated with miR-219a-2-3p inhibitor-transfected IGF-Exo for 24h followed by H 2 O 2 for 24h). (aging-us.com)
  • A screen to identify compounds that block proNGF binding and uptake into cells expressing p75 and sortilin identified lithium citrate as a potential inhibitor of proNGF and p75 NTR -mediated cell death. (eneuro.org)
  • We examined the effects of E2 on depolarization-evoked [Ca 2+ ] i in PC12 cells using fluorescence measurements. (springer.com)
  • Next, cells were collected and visualized by vibrant area and fluorescence microscopy using a Zeiss Axio Scope A1 microscope by using a CoolSnap ES CCD camera and a substantial strain mercury lamp. (micrornainhibitor.com)
  • First, upon stimulation with high glucose, a rapid shift of the redox state of individual pancreatic β-cells has been observed by fluorescence-activated cell sorting ( 7 , 8 ) as well as fluorescence microscopy techniques ( 9 , 10 ). (diabetesjournals.org)
  • Oral cavity squamous cell carcinoma (SCC) represents the most common SCC affecting the head and neck region. (pianolarge.ga)
  • Mind and throat squamous cell carcinoma (HNSCC) is the 6th most prevalent tumor in the globe. (healthweblognews.info)
  • Mind and throat squamous cell carcinoma (HNSCC) refers to a group of biologically identical malignancies that begin in the lips, tongue, dental cavity (mouth area), nose cavity (inside the nasal area), paranasal sinuses, pharynx, and larynx. (healthweblognews.info)
  • GSH, the most abundant free thiol in mammalian cells ( Meister and Andersson, 1983 ), is considered to be a major antioxidant in the brain. (jneurosci.org)
  • Cadmium is a hazardous heavy metal existing ubiquitously in the environment, but the mechanism of cadmium transport into mammalian cells has been poorly understood. (aspetjournals.org)
  • Although detrimental effects of cadmium have been well documented, the mechanism of cadmium transport, especially in mammalian cells, remains poorly understood. (aspetjournals.org)
  • However, the process of transport of each metal into mammalian cells has not yet been fully elucidated ( Nelson, 1999 ). (aspetjournals.org)
  • The specificity of the acidic organelles and their physiological significance as Ca 2+ stores are not well understood in mammalian cells, although this has been relatively better examined in lower organisms. (arvojournals.org)
  • This mechanism has the potential for uncovering a novel therapeutic target but still needs validation in a mammalian organism in an in vivo preparation where neurons exist in an environment in which both cell autonomous and cell-cell interactions have important roles. (alzforum.org)
  • 1. Cycloastragenol and astragaloside IV can suppress ROS-associated ER stress and then inhibit TXNIP/NLRP3 inflammasome activation with regulation of AMPK activity, and thereby ameliorate endothelial dysfunction by inhibiting inflammation and reducing cell apoptosis. (chemfaces.com)
  • Moreover, we were able to show that p53 contributed to SPIO-Serum-induced ferroptosis in ovarian cancer cells. (bvsalud.org)
  • Conclusion: SPIO-Serum induced ferroptosis and overexpressed p53 contributed to ferroptosis in ovarian cancer cells. (bvsalud.org)
  • However, GPCRs for lysophosphatidic acid (LPA) were not implicated in the LPE-mediated activities in LPA GPCR overexpression systems or in SK-OV3 ovarian cancer cells. (biomolther.org)
  • Nankova BB, Agarwal R, MacFabe DF, La Gamma EF (2014) Enteric Bacterial Metabolites Propionic and Butyric Acid Modulate Gene Expression, Including CREB-Dependent Catecholaminergic Neurotransmission, in PC12 Cells - Possible Relevance to Autism Spectrum Disorders. (plos.org)
  • Addition of resveratrol to the cell culture medium produced relatively low levels of hydrogen peroxide which may be a positive hormetic redox-signal for Nrf2 dependent gene expression thereby driving heme oxygenase-1 induction. (biomedcentral.com)
  • The 5' flanking region contains several consensus binding sites for transcription factors that regulate gene expression during tissue and organ development, such as myeloid zinc finger ( MZF1 ), Ikaros 2 and homeodomain proteins, as well as factors implicated in cell growth and survival responses, including AP-1 , PEA3 , E2F and NF-kB . (atlasgeneticsoncology.org)
  • Although cell fates resulting from action of the aforementioned ligands completely different, the respective gene expression profiles in early transcription are qualitatively similar, suggesting that gene expression during late transcription, but not early transcription, may reflect ligand specificity. (beds.ac.uk)
  • Mechanistically, NUPR1-mediated LCN2 expression blocks ferroptotic cell death through diminishing iron accumulation and subsequent oxidative damage. (bvsalud.org)
  • 3. A soluble proteoglycan recombinantly produced by a transformed eukaryotic cell capable of producing heparan sulfate glycosaminoglycans, which proteoglycan comprises at least one heparan sulfate attachment sequence represented by SEQ ID NO: 15, said heparan sulfate attachment sequence having covalently attached thereto a heparan sulfate glycosaminoglycan chain, wherein said proteoglycan is isolatable as a soluable form only upon the cleavage of a secretion signal peptide. (google.es)