Dothiepin: A tricyclic antidepressant with some tranquilizing action.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Multidrug Resistance-Associated Proteins: A sequence-related subfamily of ATP-BINDING CASSETTE TRANSPORTERS that actively transport organic substrates. Although considered organic anion transporters, a subset of proteins in this family have also been shown to convey drug resistance to neutral organic drugs. Their cellular function may have clinical significance for CHEMOTHERAPY in that they transport a variety of ANTINEOPLASTIC AGENTS. Overexpression of proteins in this class by NEOPLASMS is considered a possible mechanism in the development of multidrug resistance (DRUG RESISTANCE, MULTIPLE). Although similar in function to P-GLYCOPROTEINS, the proteins in this class share little sequence homology to the p-glycoprotein family of proteins.ATP-Binding Cassette Transporters: A family of MEMBRANE TRANSPORT PROTEINS that require ATP hydrolysis for the transport of substrates across membranes. The protein family derives its name from the ATP-binding domain found on the protein.Drug Resistance, Multiple: Simultaneous resistance to several structurally and functionally distinct drugs.P-Glycoprotein: A 170-kDa transmembrane glycoprotein from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS. It serves as an ATP-dependent efflux pump for a variety of chemicals, including many ANTINEOPLASTIC AGENTS. Overexpression of this glycoprotein is associated with multidrug resistance (see DRUG RESISTANCE, MULTIPLE).Trypsin Inhibitor, Kazal Pancreatic: A pancreatic trypsin inhibitor common to all mammals. It is secreted with the zymogens into the pancreatic juice. It is a protein composed of 56 amino acid residues and is different in amino acid composition and physiological activity from the Kunitz bovine pancreatic trypsin inhibitor (APROTININ).Trypsin Inhibitor, Kunitz Soybean: A high-molecular-weight protein (approximately 22,500) containing 198 amino acid residues. It is a strong inhibitor of trypsin and human plasmin.Chymotrypsin: A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.Trypsin Inhibitor, Bowman-Birk Soybean: A low-molecular-weight protein (minimum molecular weight 8000) which has the ability to inhibit trypsin as well as chymotrypsin at independent binding sites. It is characterized by a high cystine content and the absence of glycine.Aprotinin: A single-chain polypeptide derived from bovine tissues consisting of 58 amino-acid residues. It is an inhibitor of proteolytic enzymes including CHYMOTRYPSIN; KALLIKREIN; PLASMIN; and TRYPSIN. It is used in the treatment of HEMORRHAGE associated with raised plasma concentrations of plasmin. It is also used to reduce blood loss and transfusion requirements in patients at high risk of major blood loss during and following open heart surgery with EXTRACORPOREAL CIRCULATION. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1995)Drug Resistance: Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.Trypsinogen: The inactive proenzyme of trypsin secreted by the pancreas, activated in the duodenum via cleavage by enteropeptidase. (Stedman, 25th ed)Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Drug Resistance, Microbial: The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Kinetics: The rate dynamics in chemical or physical systems.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Molecular Weight: The sum of the weight of all the atoms in a molecule.Receptor, PAR-2: A G-protein-coupled, proteinase-activated receptor that is expressed in a variety of tissues including ENDOTHELIUM; LEUKOCYTES; and the GASTROINTESTINAL TRACT. The receptor is activated by TRYPSIN, which cleaves off the N-terminal peptide from the receptor. The new N-terminal peptide is a cryptic ligand for the receptor. The uncleaved receptor can also be activated by the N-terminal peptide present on the activated THROMBIN RECEPTOR and by small synthetic peptides that contain the unmasked N-terminal sequence.Peptide Hydrolases: Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Drug Resistance, Bacterial: The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Endopeptidases: A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.Drug Resistance, Neoplasm: Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Pancreas: A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.Serine Endopeptidases: Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Serine Proteinase Inhibitors: Exogenous or endogenous compounds which inhibit SERINE ENDOPEPTIDASES.Benzoylarginine Nitroanilide: A chromogenic substrate that permits direct measurement of peptide hydrolase activity, e.g., papain and trypsin, by colorimetry. The substrate liberates p-nitroaniline as a chromogenic product.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Benzamidines: Amidines substituted with a benzene group. Benzamidine and its derivatives are known as peptidase inhibitors.Bacterial Proteins: Proteins found in any species of bacterium.Pronase: A proteolytic enzyme obtained from Streptomyces griseus.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Pancreatic Elastase: A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC 3.4.21.36.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Thermolysin: A thermostable extracellular metalloendopeptidase containing four calcium ions. (Enzyme Nomenclature, 1992) 3.4.24.27.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Penicillin Resistance: Nonsusceptibility of an organism to the action of penicillins.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Papain: A proteolytic enzyme obtained from Carica papaya. It is also the name used for a purified mixture of papain and CHYMOPAPAIN that is used as a topical enzymatic debriding agent. EC 3.4.22.2.
This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide ... Protease, serine, 3 is a protein that in humans is encoded by the PRSS3 gene. This gene encodes a trypsinogen, which is a ... "Trypsin IV or mesotrypsin and p23 cleave protease-activated receptors 1 and 2 to induce inflammation and hyperalgesia". The ... "The amyloid precursor protein/protease nexin 2 Kunitz inhibitor domain is a highly specific substrate of mesotrypsin". The ...
The trypsin resistant core is composed of long amphipathic alpha helices and fuels toxic function. The hydrophobic regions of ... Cry6Aa proteins are unrelated to other insecticidal crystal proteins in primary amino acid structure. The protein is rod-shaped ... Since Cry6Aa proteins function differently than other Cry proteins, they are combined with other proteins to decrease the ... Because an organism has to be resistant to both expressed Cry proteins to survive, the chances of developing and vertically ...
... exhibits trypsin-like activity, cleaving proteins following a lysine at a specific cleavage site (Asp-Asp-Asp- ... Native enteropeptidase is resistant to soybean trypsin inhibitor. However, the isolated light chain is subtle whether prepared ... Kunitz M, Northrop JH (Jul 20, 1936). "Isolation from beef pancreas of crystalline trypsinogen, trypsin, a trypsin inhibitor, ... linked by this sequence can be cleaved by enteropeptidase to obtain the target protein following protein purification. On the ...
... while a few use the genes that encode for vegetative insecticidal proteins. Insect resistant crops target various species of ... thuringiensis is the Cowpea trypsin inhibitor (CpTI). CpTI was first approved for use cotton in 1999 and is currently ... In 2014, 154 million hectares were planted with a herbicide resistant crop and 78.8 million hectares had insect resistant. This ... Smartstax is a brand of GM maize that has eight different genes added to it, making it resistant to two types of herbicides and ...
... s also have antinutrient factors, such as trypsin inhibitors and a relatively high phytate content. Trypsin is an enzyme ... protein itself is not lost). Lentils have the second-highest ratio of protein per calorie of any legume, after soybeans. The ... The remaining 65% of the starch is a resistant starch classified as RS1. A minimum of 10% in starch from lentils escapes ... Raw lentils are 8% water, 63% carbohydrates including 11% dietary fiber, 25% protein, and 1% fat (table). Lentils are a rich ...
"Sequence and structural characteristics of the trypsin-resistant T6 surface protein of group A streptococci". J. Bacteriol. 172 ... M proteins embody a motif that is now known to be shared by many Gram-positive bacterial surface proteins. The motif includes a ... M protein is strongly anti-phagocytic and is a major virulence factor. It binds to serum factor H, destroying C3-convertase and ... M protein is a virulence factor that can be produced by certain species of Streptococcus. Viruses, parasites and bacteria are ...
The genes and proteins regulated by ulinastatin are implicated in the inflammatory process. Therefore, ulinastatin is not just ... Ulinastatin, as an urinary trypsin inhibitor (UTI), is a glycoprotein that is isolated from healthy human urine or ... Ulinastatin is an acid-resistant protease inhibitor found in human urine and released from the high-molecular-weight precursor ... Ulinastatin protein has been found in the brain, liver, kidney, gastrointestinal tract, cartilage, plasma, ovarian follicular ...
... but also helps to stabilize the enzyme and increase the half life by making it more resistant to being cut by trypsin protease ... PNMT is a protein whose encoding gene is found on chromosome 17 in humans. It consists of 4 exons and is a 30kDa protein. It ... Newer studies are also showing PNMT mRNA and protein to be expressed in other regions of the body as well. Certain neural ... RCSB Protein Data Bank. Retrieved 11 March 2015. Martin, JL; Begun, J; McLeish, MJ; Caine, JM; Grunewald, GL (October 2001). " ...
The trypsin found in pancreatin works to hydrolyze proteins into oligopeptides; amylase hydrolyzes starches into ... The formulations are generally hard capsules filled with gastro-resistant granules. Pancrelipases and pancreatins are similar, ... They help the person digest fats, starches, and proteins. Pancreatic enzymes have been used as medications since at least the ... Pancreatin contains the pancreatic enzymes trypsin, amylase and lipase. A similar mixture of enzymes is sold as pancrelipase, ...
2007). "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening ... Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only ... as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes ... which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases ...
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only ... which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases ... as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes ...
... , like trypsin, belongs to the family of serine proteases. Plasmin is released as a zymogen called plasminogen (PLG) ... In humans, the plasmin protein is encoded by the PLG gene. Plasmin is a serine protease that acts to dissolve fibrin blood ... In circulation, plasminogen adopts a closed, activation resistant conformation. Upon binding to clots, or to the cell surface, ... Plasmin is an important enzyme (EC 3.4.21.7) present in blood that degrades many blood plasma proteins, including fibrin clots ...
Ovomucoid is the major allergen from egg white and is a heat-resistant glycoprotein found to be a trypsin inhibitor. Lysozyme ... Some protein powders also use egg whites as a primary source of protein. In the 1750s, egg whites were believed to prevent ... Egg white is an alkaline solution and contains around 148 proteins. The table below lists the major proteins in egg whites by ... These denatured proteins gather together where the air and water meet and create multiple bonds with the other unraveled ...
At least six of the twelve proteins encoded by the rotavirus genome bind RNA. The role of these proteins play in rotavirus ... Non-secretors seem resistant to infection by types P[4] and P[8], indicating that blood group antigens are the receptors for ... VP4 has to be modified by the protease enzyme trypsin, which is found in the gut, into VP5* and VP8* before the virus is ... This causes the breakdown of VP7 trimers into single protein subunits, leaving the VP2 and VP6 protein coats around the viral ...
Although rich in proteins (20 %), due to less acceptable taste and flavor of cooked products, it is consumed only by the ... Horse gram is an extremely drought-resistant crop. Moderately warm, dry climatic conditions are suitable for its optimum growth ... Comparatively, horse gram seeds have higher trypsin inhibitor and hemagglutinin activities and natural phenols than most bean ... Horse gram seed is low in fat and is excellent sources of protein, dietary fiber, a variety of micro-nutrients and ...
A robust protein, OSM is stable between pH2 and 11 and resistant to heating for one hour at 56 °C. A partial amino acid ... Cleavage of the C-terminal most 31 residues at a trypsin like cleavage site yields the fully active 196 residue form. Two ... Of the proteins recruited to type I cytokine receptors STAT proteins remain the best studied. Homodimerisation of gp130 has ... Oncostatin M, also known as OSM, is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that ...
Ovomucoid is the major allergen from egg white and is a heat-resistant glycoprotein found to be a trypsin inhibitor. Lysozyme ... Visual representation of protein denaturation. A globular protein becomes unfolded when exposed to heat. ... Some protein powders also use egg whites as a primary source of protein. ... Egg white is an alkaline solution and contains around 148 proteins.[4] The table below lists the major proteins in egg whites ...
Trypsin is produced, stored and released as the inactive trypsinogen to ensure that the protein is only activated in the ... is stored in intracellular vesicles in the pancreas called zymogen granules whose membranous walls are thought to be resistant ... Once activated, the trypsin can activate more trypsinogen into trypsin. Trypsin cleaves the peptide bond on the carboxyl side ... which repels the aspartate on the back of trypsin's specificity pocket. Trypsin may also inactivate other trypsin by cleavage. ...
Optimal reactivity of gliadin occurs when the protein is partially digested by small intestinal lysozyme and trypsin into ... The IRP lies within a 25 amino-acid long region that is resistant to pancreatic proteases. The 25mer is also resistant to Brush ... HLA-DQ proteins present polypeptide regions of proteins of about 9 amino acids and larger in size (10 to 14 residues in ... assuming there is only one of these per protein and only a few genome loci with the protein. Unresolved questions relevant to a ...
... alk-SMase is resistant to trypsin digestion. Thus ENPP7 is active in the intestinal lumen and is transported along the ... Most previous studies used the name of alk-SMase for this protein. The name of ENPP7 was created based on the results of ... 2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and ... High fat diet (53% energy) greatly reduces ENPP7 activities and enzyme protein in the intestinal mucosa by 50%. On the other ...
Protein concentrate from Jatropha curcas screw-pressed seed cake and toxic and antinutritional factors in protein concentrate. ... It is resistant to a high degree of aridity, allowing it to grow in deserts. It contains phorbol esters, which are considered ... J. curcas also contains compounds such as trypsin inhibitors, phytate, saponins and a type of lectin known as curcin. The seeds ... This small tree is very resistant to aridity so it can be planted in hot and dry land in soil unsuitable for food production. ...
The bacterium produces proteins that, when ingested, kill lepidopteran caterpillars. The gene encoding for these highly toxic ... Many enzyme inhibitors in cereal grains and pulses, such as trypsin inhibitors prevalent in pulse crops, are denatured by ... Charles Valentine Riley noted that the American species Vitis labrusca was resistant to Phylloxera. Riley, with J. E. Planchon ... Certain alkaloids bind to nucleic acids and can inhibit synthesis of proteins and affect DNA repair mechanisms. Alkaloids can ...
Exine is partially hydrolyzed by trypsin and is resistant to lysozyme, in contrast to the central body. The central body can be ... "Protein-Protein Interactions in the Complex between the Enhancer Binding Protein NIFA and the Sensor NIFL from Azotobacter ... they are twice as resistant to UV light. They are also resistant to drying, ultrasound and gamma and solar irradiation, but not ... The synthesis of proteins and RNA, occurs in parallel, but it intensifies only after five hours after the addition of the ...
"Expression, purification, and characterization of Leishmania donovani trypanothione reductase in Escherichia coli". Protein ... trypsin and CaCl2". Molecular and Cellular Biochemistry. 228 (1-2): 15-23. doi:10.1023/a:1013300106220. ISSN 0300-8177. Mittal ... Recent trend of development of drug resistant field isolates and usage of alternate strategy for treatment of leishmaniasis. ... Protein Structure and Molecular Enzymology. 1548 (1): 114-120. doi:10.1016/s0167-4838(01)00223-0. Rashid, Huma; Owais, Mohammad ...
This antigen is sensitive to trypsin but resistant to chymotrypsin and Endo F. The Lewis II (Ls(a); Ge-6) antigen has insert of ... In total the glycophorins constitute ~2% of the total erythrocyte membrane protein mass. Confusingly these proteins are also ... The Ge3 epitope is encoded by exon 3. It is sensitive to trypsin but resistant to chymotrypsin, papain and pronase. It is ... It is located within exon 2 and is sensitive to trypsin and papain but resistant to chymotrypsin and pronase. ...
... s also have antinutrient factors, such as trypsin inhibitors and a relatively high phytate content. Trypsin is an enzyme ... protein itself is not lost).[16] Lentils have the second-highest ratio of protein per calorie of any legume, after soybeans. ... "resistant starch").[21] Additional resistant starch is synthesized from gelatinized starch, during cooling, after the lentils ... Raw lentils are 8% water, 63% carbohydrates including 11% dietary fiber, 25% protein, and 1% fat (table). Lentils are a rich ...
6. Role of Trypsin in Chronic Pancreatitis (CP):. Using our T-/- mice we have also explored the role of trypsin in CP (20). ... Pancreatic acinar cells are protein-synthesizing machinery for secretory proteins and any stress might lead disturbance of ... trypsinogen in vitro show that this trypsinogen variant has an increased propensity for auto-activation and is resistant to ... 4. Role of Trypsin During Acute Pancreatitis. For decades, intra-acinar trypsin activation has been considered the key event in ...
THE DNA BINDING PARAMETERS OF HYBRID TETRAMERS OF LACTOSE REPRESSOR AND THE TRYPSIN-RESISTANT CORE PROTEIN: A MODEL FOR ... The nonspecific DNA binding capacity of the lac repressor protein has been assessed by two different methods. Boundary ...
Recombinant Protein and Trypsin-resistant surface T6 protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and ... Shop Trypsin-resistant surface T6 protein ELISA Kit, ... Trypsin-resistant surface T6 protein. Trypsin-resistant surface ... Trypsin-resistant surface T6 protein Recombinant. Trypsin-resistant surface T6 protein Antibody. Also known as Trypsin- ... Protein Family Trypsin-resistant surface T6 protein. LOG IN MY ACCOUNT CART CONTENTS CHECKOUT ...
Trypsin treatment of susceptible target lymphocytes reduced entry. Entry was partially resistant to ammonium chloride. ... The HTLV receptor is a widely expressed protein.. Trejo SR1, Ratner L. ... and infection was dependent on the presence of the cleavage and fusogenic sequences in the envelope protein precursor. ...
"Sequence and structural characteristics of the trypsin-resistant T6 surface protein of group A streptococci". J. Bacteriol. 172 ... M proteins embody a motif that is now known to be shared by many Gram-positive bacterial surface proteins. The motif includes a ... M protein is strongly anti-phagocytic and is a major virulence factor. It binds to serum factor H, destroying C3-convertase and ... M protein is a virulence factor that can be produced by certain species of Streptococcus. Viruses, parasites and bacteria are ...
Trypsin from bovine pancreas for your research needs. Find product specific information including CAS, MSDS, protocols and ... resulting in a product that is resistant to autolysis. The T6567 has also been treated with TPCK to remove chymotryptic ... beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member ... For trypsin digestion of peptides, use a ratio (w:w) of about 1:100 to 1:20 for trypsin:peptide. Trypsin preparations usually ...
00am PSTMultiple aspects need to be considered when building a protein-based mass spectrometric assay such as the selection of ... 3D Spheroid Cell Models for Drug-Resistant Cancer Research DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of ... isotopic labeled protein or peptide). Proteins are reduced to peptides prior to mass spectrometric analysis and protein ... Manipulating trypsin digestion conditions to accelerate digestion and improve signal intensity for building a protein-based ...
SDS-PAGE, Coomassie staining and in-gel trypsin digestion. The protein concentrations of the lyophilised samples were measured ... A list of the total proteins, unique proteins and corresponding peptides identified resistant B. glabrata with reference to the ... Protein-protein interaction (PPI) networks were constructed between identified S. mansoni proteins (see Table 3) and the B. ... Identified proteins were subjected to BLASTp using non-redundant protein sequences of NCBI. Protein N-terminal signal sequences ...
This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide ... Protease, serine, 3 is a protein that in humans is encoded by the PRSS3 gene. This gene encodes a trypsinogen, which is a ... "Trypsin IV or mesotrypsin and p23 cleave protease-activated receptors 1 and 2 to induce inflammation and hyperalgesia". The ... "The amyloid precursor protein/protease nexin 2 Kunitz inhibitor domain is a highly specific substrate of mesotrypsin". The ...
2B, and digested with either proteinase K or trypsin, as described in Methods. Bax protein was analyzed by immunoblot with anti ... Charged amino acids in the C terminus or N terminus make Bax protease resistant. (A). Positively charged amino acids in the C- ... Cytosols prepared at different pH values were treated with proteinase K or trypsin and, as a control, trypsin digests were also ... Only the monomeric form of Bax protein could be detected in the cytosolic protein fraction, whereas in mitochondria, Bax could ...
The gliadin protein was treated with pepsin, trypsin, chymotrypsin, elastase, and carboxypeptidase. The blue- and red- colored ... Recent molecular and structural analyses of representative gluten proteins, most notably alpha- and gamma-gliadin proteins from ... Dietary gluten proteins from wheat, rye, and barley are the primary triggers for the immuno-pathogenesis of Celiac Sprue, a ... Protein sequences of the α-2 gliadin deletion mutants. (A) Deletion mutant A1234. (B) Deletion mutant A1278. The peptide ...
The remainder was intracellular and resistant to trypsin treatment. The ratio of matrix-associated to intracellular TGF-β2 was ... and total protein concentration was determined by the BCA protein assay (Pierce, Rockland, IL), and protein data were used for ... About 33% of the cell-associated TGF-β2 protein was localized in the extracellular matrix and was removed by a brief trypsin ... HPV-16 E7 Protein Down-Regulates TGF-β2 Production by a pRb-dependent Pathway.. The HPV-16 E7 protein binds to and inactivates ...
... protein phosphatase 2 phosphatase activator), Authors: Veerle Janssens. Published in: Atlas Genet Cytogenet Oncol Haematol. ... pI around 5.8; trypsin-resistant.. Expression. Both isoforms are widely expressed. In a given cell line, PTPA alpha is about ... protein phosphatase type 2A complex protein phosphatase type 2A complex protein peptidyl-prolyl isomerization peptidyl-prolyl ... protein phosphatase type 2A complex protein phosphatase type 2A complex protein peptidyl-prolyl isomerization peptidyl-prolyl ...
5 C protein includes two components: trypsin sensitive beta (β) and trypsin resistant alpha (α)5 while R includes four species ... Molecular diversity among the trypsin resistant surface proteins of group B streptococci. Zbl. Bakt. (International Journal of ... is trypsin-resistant.3 These proteins are of interest because of their possible role in GBS virulence and protective immunity. ... Ferrieri, P. Surface-localized protein antigens of group B streptococci. Rev. Infect. Dis. 10(Suppl. 2):S363-S366, 1988.PubMed ...
In order to analyze the purified protein complexes, TCA-precipitation, LysC/Trypsin digestion, and multidimensional protein ... 1974) The effect of trypsin on nuclease-resistant chromatin fragments. J. Biol. Chem. 249, 152-156. ... The histone proteins along with Ydl156w are indicated on the right side of the gel. B, Relative protein abundance for the core ... Proteins that were identified by the same set of peptides (including at least one peptide unique to such protein group to ...
It does so by acting on the proteins in the mucin to help reduce the viscosity (stickiness and gel-like nature). This makes ... especially when they are taken in gastric acid resistant capsules. If taken with meals, the proteolytic enzymes are primarily ... trypsin and chymotrypsin). ... block the enzymes so that they do not digest bodily proteins. ... Proteolytic enzymes breakdown proteins by basically adding water or hydrolyzing the bonds between specific amino acids which ...
Protein sample digestion procedure is performed at low (mildly acidic) pH to suppress artificial deamidation and disulfide bond ... To restore trypsin cleavage efficiency at lysine sites we supplemented trypsin with a special, low pH-resistant recombinant Lys ... Protein Digestion at Low pH. Trypsin and other proteases commonly used in peptide mapping sample preparation favor alkaline pH ... By supplementing trypsin with low pH resistant rLys-C we achieved efficient tryptic digestion at low pH. ...
... and partially soluble in ethylene glycol.Trypsin and chymotrysin labile.Resistant to: chondroitinases A, B and C; amylase; ... A protein and protein aggregate with high BMP activity, obtained by Step VIII, are soluble only in 6 to 8M urea or 4M GuHCl and ... The 17.5-kDa protein from human bone and the 18.5-kDa protein from bovine bone are therefore believed to be the putative BMP ... This protein has been determind to be the active protein factor of BMP derived from human bone sources and is referred to ...
In addition, although cooking soybeans normally reduces the amount of this protein, the trypsin inhibitor in GM varieties ... appears to be more heat resistant. Levels in cooked GM soy were nearly as high as those found in raw soy, and up to seven times ... scientists compare this new protein with a database of proteins known to cause allergies. The database lists the proteins ... If protein-digesting enzymes are less available, then food proteins may last longer in the gut, allowing more time for an ...
Following trypsin digestion, these proteins were characterized by mass spectrometry analysis. Functional analysis revealed that ... This work was mainly aimed to identify a novel druggable pathway useful to block TUBB3 function in drug-resistant cells. So, we ... Proteins that contain intrachain disulfide bonds will be more compact and will run above the diagonal; proteins that form ... To identify proteins forming mixed disulfide bonds with TUBB3, eluted proteins from immunoprecipitation were subjected to two- ...
Since trypsin elimination of alveolar surface protein ectodomains failed to inhibit MA formation, we interpret that alveolar ... Microinstillation of USA300WT in the protein-depleted alveoli also resulted in formation of washout-resistant MAs (Supplemental ... we eliminated all proteins from the alveolar surface by giving alveolar microinstillations of trypsin. We confirmed that these ... Substrate-binding proteins of ABC transporters, such as PhnD (27, 28), are known to play a role in bacterial adherence in vitro ...
... and then samples were digested with 15 μg of thermolysin/ml or 100 μg of trypsin/ml at 4°C for 30 min. The fragment resistant ... Interaction between srr7 S protein and soMHVR1. (A) Direct binding of soMHVR to srr7 S protein. srr7 virions were incubated ... Proteinase K digestion assay of S proteins expressed in BHK cells. Lysates of BHK cells expressing various S proteins were ... Receptor-Induced Conformational Changes of Murine Coronavirus Spike Protein Message Subject (Your Name) has forwarded a page to ...
... protein extraction, digestion and analysis. Protocols for extraction and digestion are continuously being updated and optimized ... Trypsin has become the gold standard for protein digestion to peptides for shotgun proteomics. Trypsin is a serine protease. It ... particularly proteolytically resistant myoglobin and integral membrane proteins, thereby enhancing the efficiency of their ... Trypsin is the most widely used enzyme for protein digestion. Trypsin cleaves amino acid sequences after lysine and arginine, ...
As expected, when exposed to simulated intestinal fluid, the Cry3Bb1 protein was digested to the trypsin resistant 57 kDa core ... Protein allergens are normally resistant to digestion and heat processing, unlike the Cry3Bb1 protein which was shown to ... The gene codes for a protein similar to the B. thuringiensis var. kumamotoensis insecticidal crystal protein. The protein ... protein produced in corn was shown to be equivalent to the original microbial protein. This protein is active only against ...
Protein and Enzyme Assays The Bradford [14] and the BCA protein assays (Pierce Chemical Company, Rockford, IL) were used to ... and soybean trypsin inhibitor, 21,500). NHTTerminal Sequence The NH2-terminal amino acid sequences of the purified P-450s were ... 0.73 i 0.07 nmolimg protein) was increased to 89% of the level found in the control LPR strain (0.82 -+ 0.05 nmol/mg protein), ... and 7.15 nmol/mg protein, respectively. The specific content of the P-4501,, obtained in the present study (7.60 nmoVmg protein ...
  • Since then the mechanism, site and importance of premature activation of digestive enzymes, especially trypsin, has become a major area of investigation in pathobiology of pancreatitis. (pancreapedia.org)
  • Premature activation of trypsin has been observed during both in vitro hyperstimulation and in animal models of acute pancreatitis (16, 17, 22-24). (pancreapedia.org)
  • In vitro studies on acinar cells show that within 30 min of hyper stimulation most of the active trypsin localizes to a heavy, zymogen-rich pellet and after 60 min of hyperstimulation, the trypsin activity shifts to the supernatant. (pancreapedia.org)
  • Custom ELISA Kits, Recombinant Proteins and Antibodies can be designed, manufactured and produced according to the researcher's specifications. (mybiosource.com)
  • Additionally, the epitope of anti-VWF mAb (SZ34) was mapped using recombinant proteins in combination with enzyme-linked immunosorbent assay and Western blot analysis. (jove.com)
  • Expression of Vsp1 and Vsp2 conferred glycosaminoglycan binding to recombinant B. burgdorferi cells that was similar to that observed with purified recombinant proteins and B. turicatae expressing native Vsp. (asm.org)
  • Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad. (adobecards.com)
  • Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. (sigmaaldrich.com)
  • Anchoring to the membrane can be achieved, as in ActA, by the presence of a hydrophobic stretch of about 20 amino acids followed by positively charged amino acids acting as a stop-transfer signal, at the C terminus of the protein ( 7 ). (pnas.org)
  • CHS4 encodes a 696 amino acids protein with no potential transmembrane domain. (nih.gov)
  • Bessen, D. and Fischetti, V.A, 1990, Differentiation between two biologically distinct classes of group A streptococci by limited substitutions of amino acids within the shared region of M protein-like molecules, J. Exp. (springer.com)
  • Unfortunately, extra heating damages the structure of the essential amino acids methionine and lysine and in extreme cases damages the total protein so much that it is hard to digest, assimilate and utilize by the body. (westonaprice.org)
  • At present, nine MRP proteins ranging in size from 1,325 to 1,545 amino acids have been identified in humans (hMRP1-9). (aspetjournals.org)
  • We show here that a chimeric protein composed of phaseolin and 89 amino acids of γ -zein, including the repeated and the Pro-rich domains, maintains the main characteristics of wild-type γ -zein: It is insoluble unless its disulfide bonds are reduced and forms ER-located protein bodies. (plantphysiol.org)
  • Seed storage proteins of legumes and cereals are also the major food proteins for humans, and, nutritionally, they largely complement each other: Storage proteins from legumes are poor in sulfur amino acids and those of cereal are poor in Lys and Trp. (plantphysiol.org)
  • Enteropeptidase is a type II transmembrane serine protease (TTSP) localized to the brush border of the duodenal and jejunal mucosa and synthesized as a zymogen, proenteropeptidase , which requires activation by duodenase or trypsin. (wikidoc.org)
  • The length and nature of transmembrane regions may influence whether proteins are retained in the Golgi or trafficked onwards (Munro 1995). (deepdyve.com)
  • Cargo proteins lacking their own cytosolic selection motifs can also be recruited by interacting with transmembrane mediators that contain recognition motifs for the trafficking machinery (Chavez et al. (deepdyve.com)
  • For example, mutations in or near the transmembrane segment of the kidney Cl-/HCO3- anion exchanger result in retention of the protein in the Golgi and cause distal renal tubular acidosis (Cordat et al. (deepdyve.com)
  • The transmembrane erythrocyte binding protein-175 (EBA-175) and thrombospondin-related anonymous protein (TRAP) play central roles in this process. (rupress.org)
  • Notch proteins are cell surface transmembrane-spanning receptors which mediate critically important cellular functions through direct cell-cell contact. (pubmedcentralcanada.ca)
  • Here, we present a quantitative proteomics approach extending the protein network of core histones H2A, H2B, H3, and H4 in Saccharomyces cerevisiae , among which a novel associated protein, the previously uncharacterized Ydl156w, was identified. (mcponline.org)
  • The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates. (who.int)
  • Recent molecular and structural analyses of representative gluten proteins, most notably alpha- and gamma-gliadin proteins from wheat, have improved our understanding of these pathogenic mechanisms. (nih.gov)
  • Molecular diversity among the trypsin resistant surface proteins of group B streptococci. (springer.com)
  • Molecular species of R-protein antigens produced by clinical isolates of group B streptococci. (springer.com)
  • Work with BMP material isolated from rabbit dentin matrix protein fraction, using polyacrylamide gel electrophoresis (PAGE) has been assigned a molecular weight of about 23,000. (google.ca)
  • The molecular relationships between FCT region surface proteins, pilus-like structures, and T antigens were characterized in depth, and the contributions of three FCT region-encoded surface proteins to virulence were evaluated in an animal model for superficial infection at the skin. (asm.org)
  • Molecular communication in biology is mediated by protein interactions. (nature.com)
  • The control band indicates a protein that nonspecifically cross-reacts with MFRN1 antibody and migrates at a different molecular weight. (jci.org)
  • at the same time developing protein and peptide-based tools that will allow us to manipulate cellular processes on a molecular level. (stanford.edu)
  • Thus, the complete molecular definition of an antigenic P. falciparum erythrocyte surface protein that can be used as a malaria in pregnancy vaccine has not yet been achieved. (biomedcentral.com)
  • The assembly and budding of lentiviruses, such as human immunodeficiency virus type 1 (HIV-1), are mediated by the Gag protein precursor, but the molecular details of these processes remain poorly defined. (asm.org)
  • Molecular masses and top-down analyses were used to define the alterations in the ribosomal proteins in conjunction with high coverage bottom up and middle-down analyses. (umd.edu)
  • Finally, we identified UQCRC2, a matrix-facing protein of the respiratory chain complex III, as an NLRX1-interacting molecule, thus providing a molecular basis for the role of NLRX1 in ROS generation. (biologists.org)
  • Molecular recognition is central to biology, and high-affinity binding proteins, such as antibodies, are invaluable for both diagnostics and therapeutics ( 1 ). (sciencemag.org)
  • The computer-based design of high-affinity binding proteins is a fundamental test of current understanding of the physical-chemical basis of molecular recognition and, if successful, would be a powerful complement to current library-based screening methods, because it would allow targeting of specific patches on a protein surface. (sciencemag.org)
  • The identification of actin and filamin as [ 14 C]carboplatin-binding proteins and decreased expression and disorganization of several cytoskeletal proteins in CP-r cells provide a molecular and cellular basis for the known defect in endocytosis in these cells. (aspetjournals.org)
  • Group B streptococcal Ibc protein antigen: Distribution of two determinants in wild-type strains of common serotypes. (springer.com)
  • Markers used to identify distinct strains were often based on antigenic forms of the M surface protein (M serotypes). (asm.org)
  • Our work provides a foundation for future research to develop an ECB gut-specific DNA microarray which can be used to analyze the global changes of gene expression in response to Bt protoxins/toxins and the genetic difference(s) between Bt- resistant and susceptible strains. (biomedcentral.com)
  • Susceptibility of Cry1Ab Maize-Resistant and -Susceptible Strains of S" by Liping Zhang, Fangneng Huang et al. (unl.edu)
  • Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac, and Cry1F. (unl.edu)
  • Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis . (unl.edu)
  • At least two of the six strains demonstrated a similar level (>526-fold) of resistance to Cry1Ab as shown in the known Cry1Ab-resistant strain, while resistance levels were relatively lower for other strains (116- to 129-fold). (unl.edu)
  • Two reference strains (ATCC 14579 T and ATCC 4342) and two clinical isolates (OH599 and OH600) from periodontal and endodontic infections were assessed for adherence to matrix proteins, such as type I collagen, fibronectin, laminin, and fibrinogen. (asm.org)
  • One-day-old cultures of strains OH599 and OH600 were readily ingested by PMNs in the absence of opsonins, while cells from 6-day-old cultures were resistant. (asm.org)
  • Both young and old cultures of the reference strains of B. cereus were resistant to PMN ingestion. (asm.org)
  • Preincubation of PMNs with the phagocytosis-resistant strains of B. cereus did not affect the phagocytosis of the sensitive strain. (asm.org)
  • Extraction of cells with detergent followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a major 97-kDa protein from the strains OH599 and OH600 but only a weak 97-kDa band from the reference strain ATCC 4342. (asm.org)
  • In contrast, reference strains (hydrophobicity, −1.0 to 4.2%) as well as 6-day-old cultures of clinical strains (hydrophobicity, 19.0 to 53.0%) bound in only low numbers to the proteins. (asm.org)
  • Overall, the results indicate that changes in the surface structure, evidently involving the S-layer, during growth of the clinical strains of B. cereus cause a shift from susceptibility to PMN ingestion and strong binding to matrix and basement membrane proteins. (asm.org)
  • While attempting to validate the role of M.tb GAPDH as a transferrin receptor we were unable to obtain sufficient quantities of stable recombinant protein using E. coli [ 10 ] or M. smegmatis as host strains. (biomedcentral.com)
  • Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1, (who.int)
  • On the other hand, an equimolar mixture of the second and third domains of OMJPQ inhibited bovine trypsin more strongly than the corresponding native OMJPQ did. (go.jp)
  • The classical understanding of why a GM crop might create new allergies is that the imported genes produce a new protein, which has never before been present. (organicconsumers.org)
  • More recent analysis of the emm genes encoding M proteins led to the definition of three emm genotype patterns, based on the number of emm genes, their ancestral lineages, and their chromosomal arrangements ( 29 , 30 ). (asm.org)
  • Genes encoding pilus-associated proteins map to the FCT region of Streptococcus pyogenes ( 28 ), a highly diversified portion of the genome that contains several well-studied genes ( cpa , prtF1 , and prtF2 ) encoding microbial surface cell recognition adhesion matrix molecules (MSCRAMMs) ( 5 , 12 , 21 , 32 ). (asm.org)
  • The T6 protein and other FCT region gene products are anchored to the peptidoglycan cell wall via specialized sortases, whose genes also lie within the FCT region ( 1 , 2 ). (asm.org)
  • Differential expressions of 15 out of the 41 selected candidate genes examined by RT-PCR, including 5 genes with apparently decreased expression and 10 with increased expression in Cry1Ab-resistant strain, may help us conclusively identify the candidate genes involved in Bt resistance and provide us with new insights into the mechanism of Cry1Ab resistance in ECB. (biomedcentral.com)
  • The M. hyopneumoniae strain 232 genome is one of the smallest and best annotated microbial genomes, containing only 728 annotated genes and 691 known proteins. (biomedcentral.com)
  • Proteogenomic mapping showed that the protein coding genes of the M. hyopneumoniae strain 232 identified in this study are well annotated. (biomedcentral.com)
  • Experiments that used genes encoding wild-type or mutated storage proteins to improve by genetic engineering the nutritional value of seeds or vegetative plant tissues indicated that the subcellular location of these proteins is important for stable accumulation (for review, see Tabe and Higgins, 1998 ). (plantphysiol.org)
  • We observed that levels of phosphorylation and glycosylation of TUBB3 are associated with the resistant phenotype and compartmentalization into cytoskeleton. (aacrjournals.org)
  • Our data highlight that compartmentalization into mitochondrion and cytoskeleton is associated to a different pathway of posttranslational changes and that glycosylation and phosphorylation status of TUBB3 correlates with the paclitaxel-resistant phenotype. (aacrjournals.org)
  • Both interaction among these proteins and tyrosine phosphorylation of DMPK are increased under oxidative stress, and Src inhibition selectively enhances death in DMPK-expressing cells after HK II detachment from the mitochondria. (nature.com)
  • Although P-gp and MRP1 have many functional similarities and are both members of the ATP-binding cassette superfamily of proteins, they possess only a 15% amino acid homology ( Cole and Deeley, 1998 ). (aspetjournals.org)
  • Nonetheless, expression of either protein is associated with reduced drug accumulation within cells and with the development of drug resistance ( Cole and Deeley, 1998 ). (aspetjournals.org)
  • All storage proteins are translocated cotranslationally into the lumen of the endoplasmic reticulum (ER), and then they either accumulate therein or traffic through the secretory pathway to storage vacuoles (for review, see Müntz, 1998 ). (plantphysiol.org)
  • Storage proteins of the 7S and 11S classes, which are particularly abundant in legumes, accumulate in protein storage vacuoles, whereas cereal prolamins are mainly located in the ER ( Müntz, 1998 ). (plantphysiol.org)
  • The contribution of this term was quite different for various protein substrates-it was positive for β-LG and negative for β-casein. (mdpi.com)
  • Sortase protein substrates are first synthesized in the cytoplasm as a full-length precursor (P1) containing an N-terminal signal peptide (SP) and a C-terminal sorting signal. (asmscience.org)
  • However plasma B cells can generate antibodies against M protein which will help in opsonization and further the destruction of the microorganism by the macrophages and neutrophils. (wikipedia.org)
  • Cross-reactivity of anti-M protein antibodies with heart muscle has been suggested to be the basis for rheumatic fever. (wikipedia.org)
  • This study investigates whether chondroitin sulphate A (CSA) binding parasites express trypsin-resistant variant surface antigens (VSA) that bind female-specific antibodies induced as a result of pregnancy associated malaria (PAM). (biomedcentral.com)
  • P. falciparum infected erythrocytes selected for adhesion to CSA were found to express trypsin-resistant VSA that are the target of naturally acquired antibodies from pregnant women living in a malaria endemic region of Ghana. (biomedcentral.com)
  • Lactose, fibronectin, laminin, and antibodies against the S-protein reduced binding to laminin but not to fibronectin. (asm.org)
  • Current methods for producing antibodies and other proteins that bind a protein of interest involve screening large numbers of variants generated by the immune system or by library construction ( 2 ). (sciencemag.org)
  • Therefore, we have shown that by either cytokine withdrawal, experimental manipulation of pH, or site-directed mutagenesis, Bax protein changes conformation, exposing membrane-seeking domains, thereby inducing mitochondrial translocation and initiating the cascade of events leading to apoptotic death. (pnas.org)
  • Also known as Trypsin-resistant surface T6 protein (T6 antigen). (mybiosource.com)
  • Although it is an integral part of the T-antigen complex, the fibronectin-binding protein PrtF2 is not covalently linked to the FctA- and Cpa-containing heteropolymer derived from cell extracts. (asm.org)
  • enhanced aromatic sequon, N-linked glycosylation, O-linked glycosylation, subcellular localization, Tn antigen Introduction Successful protein folding and appropriate post-translational modifications (PTMs) allow trafficking along the secretory pathway, whereas defects in maturation result in protein degradation by quality control mechanisms. (deepdyve.com)
  • Ferrieri P., Flores A.E. (1997) Surface Protein Expression in Group B Streptococcal Invasive Isolates. (springer.com)
  • However, the isolated light chain is subtle whether prepared by limited reduction of the natural protein or by mutagenesis and expression in COS cells . (wikidoc.org)
  • E ) Western blot analysis shows specific expression of TMEM14C protein in differentiating TER119 + murine fetal liver erythroid cells. (jci.org)
  • The enforced expression of NCA-90 protein in HeLa cells stably transfected with NCA-90 cDNA did not result in increased resistance of the transfected cells to daunorubicin or a decrease in daunorubicin accumulation in the transfected cells compared to cells transfected only with the expression vector. (aacrjournals.org)
  • 377-382, 1997) of diminished accumulation, retention, and cytotoxicity of doxorubicin in EJNIH3T3 cells in which enforced expression of CEA was accomplished leaves open the possibility that the overexpression of CEA, possibly in combination with that of NCA-90, could account at least in part for the drug resistant phenotype displayed by MCF-7/AdrVp cells. (aacrjournals.org)
  • Moreover, trypsin- and thrombin-induced upregulated expression of TNF was observed in CD4+, IL-4+, or CD25+ T cells, but not in IFN+ or IL-17+ T cells. (hindawi.com)
  • In our previous study [ 12 ], we have showed the expression of PAR-1, PAR-2, and PAR-3 on T cells, and thrombin-, trypsin-, and tryptase-induced interleukin (IL-6) release from T cells. (hindawi.com)
  • Brain expression of the multidrug resistance proteins (MRPs), a collection of membrane-associated ATP-dependent efflux transporters, is poorly understood. (aspetjournals.org)
  • Although several studies have examined the expression of these proteins within the brain barriers (i.e., the blood-brain barrier and choroid plexus), little information is available with respect to brain parenchyma cells such as microglia and astrocytes. (aspetjournals.org)
  • Although MRP1 expression is most often associated with multidrug resistance to cancer chemotherapy agents, antiretroviral agents used in the treatment of human immunodeficiency virus-1 (HIV-1) infection may also interact with these proteins. (aspetjournals.org)
  • Decreased expression of these two proteins was found in two different human CP-r cell lines (KB-CP20 and 7404-CP20), in comparison with their parental cell lines (KB-3-1 and BEL-7404), respectively. (aspetjournals.org)
  • Transfection of a wild-type actin-enhanced green fluorescent protein (EGFP) expression vector into 7404-CP20 cells resulted in a nonfilamentous actin-EGFP distribution compared with a normal distribution in the cisplatin-sensitive BEL-7404 cells, suggesting that cytoskeletal organization is disturbed in the CP-r cells. (aspetjournals.org)
  • In addition, expression of such conserved multisubunit proteins poses the problem of heteromerization with host homologues. (biomedcentral.com)
  • Our studies establish that the attenuated strain M.tb H37Ra is a suitable host for the expression of highly hydrophobic, conserved, multimeric proteins of M.tb H37Rv. (biomedcentral.com)
  • Significantly, this expression host overcomes the limitations of E. coli and M. smegmatis expression and yields recombinant protein that is qualitatively superior to that obtained by traditional methods. (biomedcentral.com)
  • The current study demonstrates that M.tb H37Ra can be utilized as a host for the successful expression and purification of recombinant M.tb H37Rv proteins. (biomedcentral.com)
  • The majority of recombinant protein was localized in the cytosol as opposed to significant incorporation into the membrane and cell wall fractions upon expression in E. coli or M. smegmatis . (biomedcentral.com)
  • In addition as GAPDH is a highly conserved multisubunit protein we also observed that expression in M. smegmatis resulted in the formation of a chimeric protein incorporating both host and vector components. (biomedcentral.com)
  • Transforming alleles of Notch direct the expression of truncated proteins that primarily consist of N ic and are not tethered to the plasma membrane. (pubmedcentralcanada.ca)
  • Our lab has demonstrated that an E1A-immortalized baby rat kidney cell line (RKE) can be directly transformed by ectopic expression of constitutive alleles of Notch1 and Notch2 (N1 ic and N2 ic ), indicating these proteins have a direct role in the transformation of cells. (pubmedcentralcanada.ca)
  • The principal theme of these investigations concerns the inhibited state of mature unfertilized sea urchin eggs with respect to uridine uptake and protein synthesis. (caltech.edu)
  • Tests with puromycin show that protein synthesis is unnecessary for the generation of uridine uptake after fertilization. (caltech.edu)
  • 14 ] have shown that NPP inhibitors such as furosemide [ 4 , 15 ] prevent the uptake of sulpho-NHS-LC-biotin and subsequently the biotinylation of internal proteins. (biomedcentral.com)
  • Cisplatin resistant (CP-r) cells often show decreased uptake of cisplatin in association with reduced cell surface proteins and decreased endocytosis. (aspetjournals.org)
  • The current model for the uptake of heme-iron by the Isd proteins states that IsdA, IsdB, and IsdH would interact with host hemoproteins such as hemoglobin (Hb), haptoglobin (Hpt), and/or hemopexin, which are released after erythrocyte lysis. (asmscience.org)
  • To get insights into the mechanisms by which this protein is involved in drug resistance, we analyzed TUBB3 in a panel of drug-sensitive and drug-resistant cell lines. (aacrjournals.org)
  • The primary limitation of efficacy for paclitaxel is the development of drug-resistant cancer cells that exhibit the multidrug resistance phenotype ( 3 ). (aacrjournals.org)
  • Because of the breakdown of malaria control programs, the constant emergence of drug resistant parasites, and, possibly, climatic changes malaria poses a major problem for the developing countries. (semanticscholar.org)
  • Epidemiology of drug-resistant malaria. (semanticscholar.org)
  • Comparative densitometry imaging software indicated the presence of two novel protein spots in the drug resistant cell line as well six additional spots with increased and decreased abundances. (umd.edu)
  • Sample preparation (protein and drug-resistant L. tropica parasites extraction) niasis, early diagnosis and appropriate . (who.int)
  • Susceptible B. glabrata several proteins correlated with immunity and anti-inflammation, such as glutathione S-transferase and zinc metalloproteinase, and S. mansoni sporocyst presence. (springer.com)
  • It is more likely that immunization with only a few parasite proteins cannot duplicate the immunity elicited by exposure to a parasite that has thousands of proteins. (jyi.org)
  • In contrast, less is known about proteins that constitutively or predominantly interact with histones independent of the chromatin state. (mcponline.org)
  • Through TUBB3 immunoprecipitation, we isolated protein species able to interact with TUBB3. (aacrjournals.org)
  • Even proteins that are disordered under physiological conditions or that contain large unstructured regions commonly interact with well-structured binding sites on other biomolecules. (nature.com)
  • Pulse-chase labeling confirms the high affinity of BiP to insoluble zeolin but indicates that, unlike structurally defective proteins that also extensively interact with BiP, zeolin is highly stable. (plantphysiol.org)
  • p95 is also highly expressed in multidrug-resistant NCI-H1688 cells derived from a human small cell lung carcinoma. (aacrjournals.org)
  • Merozoites of Plasmodium falciparum , the parasite responsible for the most dangerous form of malaria, invade erythrocytes using a family of adhesins called Duffy binding ligand-erythrocyte binding proteins (DBL-EBPs). (rupress.org)
  • Two main observations pointed to the role of the LPXTG motif in anchoring proteins to the cell surface. (pnas.org)
  • Viruses, parasites and bacteria are covered in protein and sugar molecules that help them gain entry into a host by counteracting the host's defences. (wikipedia.org)
  • This study identifies discordance between the trypsin sensitivity of CSA binding and surface recognition of CSA selected parasites by serum IgG from malaria exposed pregnant women. (biomedcentral.com)
  • The Cry6 family of proteins from Bacillus thuringiensis represents a group of powerful toxins with great potential for use in the control of coleopteran insects and of nematode parasites of importance to agriculture. (biomedcentral.com)
  • F1 resistant B. glabrata ESPs were predominately associated with anti-pathogen activity and detoxification, such as leukocyte elastase and peroxiredoxin. (springer.com)
  • Trypsin processing was found to result in the loss of some internal sequences while the C-terminal region remains disulfide-linked to the main core of the toxin. (biomedcentral.com)
  • Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca 2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. (sigmaaldrich.com)
  • Solutions are stable for approximately 1 year when aliquoted and stored at -20°C. The presence of Ca2+ (20 mM) will also retard trypsin's ability to autodigest, and will therefore help to maintain the stability of the trypsin in solution. (sigmaaldrich.com)
  • Based on the limited number of proteins that have been identified as SUMO targets, SUMO has been implicated to be involved in protein transport, transcription regulation, protein stability, and localization to nuclear bodies ( 1 ). (mcponline.org)
  • We synthesized seven members of the family in a rabbit reticulocyte lysate, determined their Stokes radius, sedimentation coefficient, and frictional ratio, and assayed their stability to trypsin. (embl-heidelberg.de)