In eukaryotes, a genetic unit consisting of a noncontiguous group of genes under the control of a single regulator gene. In bacteria, regulons are global regulatory systems involved in the interplay of pleiotropic regulatory domains and consist of several OPERONS.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Proteins found in any species of bacterium.
A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
The functional hereditary units of BACTERIA.
Proteins obtained from ESCHERICHIA COLI.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A species of gram-positive bacteria that is a common soil and water saprophyte.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
A transcriptional regulator in prokaryotes which, when activated by binding cyclic AMP, acts at several promoters. Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. It was subsequently shown to regulate several functions unrelated to catabolism, and to be both a negative and a positive regulator of transcription. Cell surface cyclic AMP receptors are not included (CYCLIC AMP RECEPTORS), nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins, which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES.
An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
The genetic complement of a BACTERIA as represented in its DNA.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
Inorganic salts of phosphoric acid.
The regulatory elements of an OPERON to which activators or repressors bind thereby effecting the transcription of GENES in the operon.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.
A constellation of responses that occur when an organism is exposed to excessive heat. Responses include synthesis of new proteins and regulation of others.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Proteins that bind to and are involved in the metabolism of phosphate ions.
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
Genes that show rapid and transient expression in the absence of de novo protein synthesis. The term was originally used exclusively for viral genes where immediate-early referred to transcription immediately following virus integration into the host cell. It is also used to describe cellular genes which are expressed immediately after resting cells are stimulated by extracellular signals such as growth factors and neurotransmitters.
The processes whereby the internal environment of an organism tends to remain balanced and stable.
Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Comprehensive, methodical analysis of complex biological systems by monitoring responses to perturbations of biological processes. Large scale, computerized collection and analysis of the data are used to develop and test models of biological systems.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
The aggregate business enterprise of agriculture, manufacture, and distribution related to tobacco and tobacco-derived products.
A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.
An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.

PhoP-PhoQ-regulated loci are required for enhanced bile resistance in Salmonella spp. (1/1176)

As enteric pathogens, Salmonella spp. are resistant to the actions of bile. Salmonella typhimurium and Salmonella typhi strains were examined to better define the bile resistance phenotype. The MICs of bile for wild-type S. typhimurium and S. typhi were 18 and 12%, respectively, and pretreatment of log-phase S. typhimurium with 15% bile dramatically increased bile resistance. Mutant strains of S. typhimurium and S. typhi lacking the virulence regulator PhoP-PhoQ were killed at significantly lower bile concentrations than wild-type strains, while strains with constitutively active PhoP were able to survive prolonged incubation with bile at concentrations of >60%. PhoP-PhoQ was shown to mediate resistance specifically to the bile components deoxycholate and conjugated forms of chenodeoxycholate, and the protective effect was not generalized to other membrane-active agents. Growth of both S. typhimurium and S. typhi in bile and in deoxycholate resulted in the induction or repression of a number of proteins, many of which appeared identical to PhoP-PhoQ-activated or -repressed products. The PhoP-PhoQ regulon was not induced by bile, nor did any of the 21 PhoP-activated or -repressed genes tested play a role in bile resistance. However, of the PhoP-activated or -repressed genes tested, two (prgC and prgH) were transcriptionally repressed by bile in the medium independent of PhoP-PhoQ. These data suggest that salmonellae can sense and respond to bile to increase resistance and that this response likely includes proteins that are members of the PhoP regulon. These bile- and PhoP-PhoQ-regulated products may play an important role in the survival of Salmonella spp. in the intestine or gallbladder.  (+info)

Induction of the soxRS regulon of Escherichia coli by superoxide. (2/1176)

The soxRS regulon orchestrates a multifaceted defense against oxidative stress, by inducing the transcription of approximately 15 genes. The induction of this regulon by redox agents, known to mediate O-2 production, led to the view that O-2 is one signal to which it responds. However, redox cycling agents deplete cellular reductants while producing O-2, and one may question whether the regulon responds to the depletion of some cytoplasmic reductant or to O-2, or both. We demonstrate that raising [O-2] by mutational deletion of superoxide dismutases and/or by addition of paraquat, both under aerobic conditions, causes induction of a member of the soxRS regulon and that a mutational defect in soxRS eliminates that induction. This establishes that O-2, directly or indirectly, can cause induction of this defensive regulon.  (+info)

Mutational analysis of the phoD promoter in Bacillus subtilis: implications for PhoP binding and promoter activation of Pho regulon promoters. (3/1176)

The PhoP-PhoR two-component regulatory system controls the phosphate deficiency response in B. subtilis. A number of Pho regulon genes which require PhoP approximately P for activation or repression have been identified. The studies reported here were initiated to understand the PhoP-DNA interaction necessary for Pho promoter regulation. The regulatory region of phoD was characterized in detail using oligo-directed mutagenesis, DNase I footprinting, and in vivo transcription assays. These data reveal basic principles of PhoP binding relevant to PhoP's interaction with other Pho regulon promoters. Our results show that: (i) a dimer of PhoP approximately P is able to bind two consensus repeats in a stable fashion; (ii) PhoP binding is highly cooperative within the core promoter region, which is located from -66 to -17 on the coding strand and contains four TT(A/T/C)ACA-like repeats; (iii) specific bases comprising the TT(A/T/C)ACA consensus are essential for transcriptional activation, but the specific base pairs of the intervening sequences separating the consensus repeats are not important for either PhoP binding or promoter activation; (iv) the spacing between two consensus repeats within a putative dimer binding site in the core region is important for both PhoP binding and promoter activation; (v) the exact spacing between two dimer binding sites within the core region is important for promoter activation but less so for PhoP binding affinity, as long as the repeats are on the same face of the helix; and (vi) the 5' secondary binding region is important for coordinated PhoP binding to the core binding region, making it nearly essential for promoter activation.  (+info)

Nitroreductase A is regulated as a member of the soxRS regulon of Escherichia coli. (4/1176)

Nitroreductase A catalyzes the divalent reduction of nitro compounds, quinones, and dyes by NADPH. In this paper, nitroreductase A is induced in Escherichia coli by exposure to paraquat in a manner that depends on the expression of soxR. Nitroreductase activity was only slightly induced by paraquat in a strain bearing a mutational defect in the gene encoding nitroreductase A, but it was approximately 3-fold induced in the parental strain. Nitroreductase A thus appears to be a member of the soxRS regulon and probably contributes to the defenses against oxidative stress by minimizing the redox cycling attendant upon the univalent reduction of nitro compounds, quinones, and dyes.  (+info)

Acarbose, a pseudooligosaccharide, is transported but not metabolized by the maltose-maltodextrin system of Escherichia coli. (5/1176)

The pseudooligosaccharide acarbose is a potent inhibitor of amylases, glucosidases, and cyclodextrin glycosyltransferase and is clinically used for the treatment of so-called type II or insulin-independent diabetes. The compound consists of an unsaturated aminocyclitol, a deoxyhexose, and a maltose. The unsaturated aminocyclitol moiety (also called valienamine) is primarily responsible for the inhibition of glucosidases. Due to its structural similarity to maltotetraose, we have investigated whether acarbose is recognized as a substrate by the maltose/maltodextrin system of Escherichia coli. Acarbose at millimolar concentrations specifically affected the growth of E. coli K-12 on maltose as the sole source of carbon and energy. Uptake of radiolabeled maltose was competitively inhibited by acarbose, with a Ki of 1.1 microM. Maltose-grown cells transported radiolabeled acarbose, indicating that the compound is recognized as a substrate. Studying the interaction of acarbose with purified maltoporin in black lipid membranes revealed that the kinetics of acarbose binding to LamB is asymmetric. The on-rate of acarbose is approximately 30 times lower when the molecule enters the pore from the extracellular side than when it enters from the periplasmic side. Acarbose could not be utilized as a carbon source since the compound alone was not a substrate of amylomaltase (MalQ) and was only poorly attacked by maltodextrin glucosidase (MalZ).  (+info)

In vivo transcription of the Escherichia coli oxyR regulon as a function of growth phase and in response to oxidative stress. (6/1176)

Simultaneous expression of seven genes in Escherichia coli was measured by a reverse transcription-multiplex PCR fluorescence procedure. Genes studied were (i) oxyR (transcriptional regulator); (ii) katG, dps, gorA, and ahpCF (controlled by OxyR); (iii) sodA (controlled by SoxRS); and (iv) trxA (not related to OxyR or SoxRS). Except for trxA, transcription of all genes was activated during the course of growth of wild-type bacteria, though notable variations were observed with respect to both the time and extent of activation. Whereas oxyR, katG, dps, and gorA were activated during exponential growth, ahpCF and sodA were stimulated in stationary phase. Maximal induction ranged from 4.6- to 86.5-fold, for gorA and dps, respectively. Treatment with H2O2 stimulated expression of the genes (katG, dps, ahpCF, and gorA) previously identified as members of the OxyR regulon, except for oxyR itself. Induction by H2O2 was a remarkably rapid and reversible process that took place in an OxyR-dependent and sigmaS-independent manner. NaCl induced expression of the genes controlled by OxyR, including the oxyR locus. This transcriptional up-regulation was preserved in a strain with the DeltaoxyR::kan mutation, but it was abolished (ahpCF) or significantly reduced (oxyR and dps) in a strain with the rpoS::Tn10 mutation, potentially reflecting positive transcriptional regulation of the oxyR regulon by sigmaS. Expression of trxA was not increased either by H2O2 stress or by a shift to high-osmolarity conditions.  (+info)

Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis. (7/1176)

Bacillus subtilis responds to various stimuli (heat, ethanol and salt stress, energy starvation) with the induction of general stress proteins (GSPs). Most of them belong to the stress and stationary-phase regulon controlled by the alternative sigma factor sigmaB. The majority of sigmaB-dependent proteins are thought to provide a precautionary general stress resistance in stressed or starved cells. In this report, the identification and transcriptional analysis of nine new members of the sigmaB regulon are described. The biochemical function was not determined for any of the proteins encoded by the nine new sigmaB-dependent stress genes, however, similarities to proteins in the databases allowed a distinction between proteins with putative (i-iv) and unknown (v) function. The putative functions of BmrU, YcdF, YdaD, YdaP, YhdN and YocK underline the suggested protective role of sigmaB-dependent GSPs and also elucidate new areas where sigmaB might play an important role. (i) The finding that the bmrUR operon is under sigmaB control indicates that the elimination of multidrug compounds might be a new function in multiple stress resistance. (ii) YcdF and YdaD resemble NAD(P)-dependent dehydrogenases. Both proteins could be involved in the generation of NAD(P)H and therefore in the maintenance of the intracellular redox balance under stress. (iii) The ydaP gene might belong to the increasing number of sigmaB-dependent genes whose orthologues are under the control of sigmas in Escherichia coli, indicating that both regulons may fulfil similar functions. (iv) YhdN shows weak similarities to potassium ion channel proteins and YocK shows resemblance to the DnaK suppressor protein DksA. (v) Three new sigmaB-dependent genes (ydaE, ydaG and yfkM) encoding proteins with still unknown functions were also described. Further analyses of corresponding mutants might allow a first prediction of their function within the framework of the general stress regulon.  (+info)

A HilA-independent pathway to Salmonella typhimurium invasion gene transcription. (8/1176)

Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outside (SirA) SPI1. Two unique loci, sirB and sirC, which contribute to SPI1 gene transcription were defined. sirC is an SPI1-encoded transcription factor of the AraC family that contributes to the invasive phenotype. sirB is required for maximal expression of sirC and consists of two open reading frames located near kdsA, a gene involved in lipopolysaccharide biosynthesis. sirC expression, unlike expression of other SPI1 genes, does not require HilA. Overexpression of sirC or sirA restores expression of a subset of SPI1 genes, including invF and sspC, in the absence of HilA. These data define roles for SirC and SirA as part of a HilA-independent pathway to SPI1 gene expression. We postulate that HilA-independent activation of inv expression is important for efficient assembly and function of the SPI1 TTSS.  (+info)

The soxR locus of Escherichia coli K12 mediates transcriptional activation of a complex oxidative stress regulon in response to superoxide-generating (redox-cycling) agents. We have cloned the soxR locus, which is positioned near the uvrA gene at 92.2 min on the genetic map, by monitoring complement …
This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
A functional ovarian-specific PAX8-centric regulon is susceptible to FDA-approved HDAC inhibitors, providing the rationale to target human cancers driven by lineage-survival oncogenes with epigenetic therapeutics perturbing the enhancer topology.
Protein Involved In Negative Regulation Of Iron Regulon Transcription; Forms An Iron Independent Complex With Fra2p, Grx3p, And Grx4p; Cytosolic; Mutant Fails To Repress Transcription Of Iron Regulon And Is Defective In Spore Formation
Protein Involved In Negative Regulation Of Iron Regulon Transcription; Forms An Iron Independent Complex With Fra2p, Grx3p, And Grx4p; Cytosolic; Mutant Fails To Repress Transcription Of Iron Regulon And Is Defective In Spore Formation
Many other examples can be found in this review by Jack Keene. I dont think Ive seen an example of this yet, but given the slight wobble in microRNA specificity, one could imagine a single microRNA regulating a whole set of genes. Also, most interesting for my neuro-tastes is the recent report from the Moore lab showing that the immediate-early gene implicated in neuronal homeostasis, Arc, may be part of a regulon defined by introns in the 3UTR. The mechanism is just too clever but requires an explication on the pioneer round of translation. Basically the cell tricks itself into thinking it made a funky RNA and destroys it after one round of synthesis. The other RNAs regulated in this path in neurons must have opposing effects to Arc though because knocking down this negative regulation pathway led to increased excitability (increased Arc reduces neuronal excitability). This raises a more general question. The idea of RNA regulons is nice, but how much can you predict knowing that your gene ...
3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.4 - complex regulation --> 3.1.2.4.2 - regulons or multilayer component regulatory systems ...
Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.3) ...
Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 117.7) ...
Abstract: Approximately 1.7 billion population of the world contain latent Mycobacterium tuberculosis (Mtb) with a substantial risk of progression to clinical outcome. Containment of seed beds of Mtb is essential to eliminate tuberculosis completely in high burden settings like India. Hence, there is an urgent need for the development of putative serological markers for detection or vaccine candidates to prevent latent tuberculosis infection (LTBI). DosR regulon antigens of Mtb might serve as attractive targets for LTBI diagnosis or vaccine development as they are specifically expressed and are up-regulated during latent phase. In this study, we investigated the role of Rv2004c, a member of DosR regulon (exclusive to Mtb complex), in host pathogen interactions and its immunogenic potential in LTBI, active TB and healthy control cohorts. Rv2004c elicited strong antibody response in individuals with LTBI compared to active TB patients and healthy cohorts. Recombinant Rv2004c induced pro-inflammatory
Microbial biofilm formation is thought of as a developmental pathway, whereby cells progress through environmentally regulated and temporally distinct stages during transition from free-swimming lifestyles to members of a structured surface-attached consortium. The focus of my doctoral studies has been to understand how environmental cues are sensed by P. fluorescens and integrated with mechanisms to regulate an important step in biofilm formation, the transition to committed association with the surface. Low levels of extracellular inorganic phosphate (P i ) were shown to inhibit stable surface attachment by P. fluorescens. Activation of the Pho regulon in P i -limiting conditions was shown to be both necessary and sufficient for inhibition of biofilm formation. The protein product of the Pho regulon gene rapA cleaves the intracellular signaling molecule c-di-GMP. Reductions in the levels of c-di-GMP were shown to inhibit both secretion and localization of LapA to the outer membrane. LapA is a ...
Escherichia coli responds to the redox stress imposed by superoxide-generating agents such as paraquat by activating the synthesis of as many as 80 polypeptides. Expression of a key group of these inducible proteins is controlled at the transcriptional level by the soxRS locus (the soxRS regulon). A two-stage control system was hypothesized for soxRS, in which an intracellular redox signal would trigger the SoxR protein as a transcriptional activator of the soxS gene and the resulting increased levels of SoxS protein would activate transcription of the various soxRS regulon genes (B. Demple and C.F. Amábile Cuevas, Cell 67:837-839, 1990). We have constructed operon fusions of the E. coli lac genes to the soxS promoter to monitor soxS transcription. Expression from the soxS promoter is strongly inducible by paraquat in a manner strictly dependent on a functional soxR gene. Several other superoxide-generating agents also trigger soxR(+)-dependent soxS expression, and the inductions by paraquat ...
TY - JOUR. T1 - A Multi-Serotype Approach Clarifies the Catabolite Control Protein A Regulon in the Major Human Pathogen Group A Streptococcus. AU - Debroy, Sruti. AU - Saldaña, Miguel. AU - Travisany, Dante. AU - Montano, Andrew. AU - Galloway-Peña, Jessica. AU - Horstmann, Nicola. AU - Yao, Hui. AU - González, Mauricio. AU - Maass, Alejandro. AU - Latorre, Mauricio. AU - Shelburne, Samuel A.. PY - 2016/9/1. Y1 - 2016/9/1. N2 - Catabolite control protein A (CcpA) is a highly conserved, master regulator of carbon source utilization in gram-positive bacteria, but the CcpA regulon remains ill-defined. In this study we aimed to clarify the CcpA regulon by determining the impact of CcpA-inactivation on the virulence and transcriptome of three distinct serotypes of the major human pathogen Group A Streptococcus (GAS). CcpA-inactivation significantly decreased GAS virulence in a broad array of animal challenge models consistent with the idea that CcpA is critical to gram-positive bacterial ...
View Notes - DNA REPAIR from BIO 326R at University of Texas. approximate positions of some of the genes of the SOS regulon are shown. SOS RESPONSE 20 Trans-lesion synthesis by DNA POL V (A) Helicase
Galactose Regulon of Yeast: From Genetics to Systems Biology, 9781281241863, available at Book Depository with free delivery worldwide.
The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell in the absence of appropriate negative regulatory elements encoded by the korA, korB, korC, and korE determinants. We have proposed that the functions of the kil loci are related to RK2 maintenance or host range. Here, we report the nucleotide sequence of a 2.44-kb region that includes the lethal kilB determinant. We identified the first three genes of the kilB operon (designated klbA, klbB, and klbC), and we determined by deletion analysis that the host-lethal phenotype requires klbB. The predicted amino acid sequence of the 34,995-Da klbA product reveals a potential ATP-binding fold. The klbB product is predicted to be a membrane protein with a molecular mass of 15,012 Da with ...
Bennett, H. J., Pearce, D. M., Glenn, S., Taylor, C. M., Kuhn, M., Sonenshein, A. L., …Roberts, I. S. (2007). Characterization of relA and codY mutants of Listeria monocytogenes: identification of the CodY regulon and its role in virulence. Molecular Microbiology. 63, 1453--1467. doi:10.1111/j.1365-2958.2007.05597.x. ISSN 0950-382X. ...
Here we present a successful transplantation of the GAL genetic regulatory circuitry into the G-protein signaling pathway in yeast. The GAL regulon represents a strictly regulated transcriptional mechanism that we have transplanted into yeast to create a highly robust induction system to assist the detection of on-off switching in G-protein signaling. In our system, we engineered yeast to drive th ...
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We assigned a putative transcriptional start site to this promoter based on the observation that the majority of the promoters, whose transcriptional start sites were determined experimentally, present a distance of 6 nucleotides between the transcriptional start site and the -10 box 3550697. ...
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Background. Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated.. Results. Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was ...
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Bioconductor version: Release (3.6) RTNsurvival is a tool for integrating regulons generated by the RTN package with survival information. For a given regulon, the 2-tailed GSEA approach computes a differential Enrichment Score (dES) for each individual sample, and the dES distribution of all samples is then used to assess the survival statistics for the cohort. There are two main survival analysis workflows: a Cox Proportional Hazards approach used to model regulons as predictors of survival time, and a Kaplan-Meier analysis assessing the stratification of a cohort based on the regulon activity. All plots can be fine-tuned to the users specifications.. Author: Clarice S. Groeneveld, Vinicius S. Chagas, Mauro A. A. Castro Maintainer: Clarice Groeneveld ,clari.groeneveld at gmail.com,, Mauro A. A. Castro ,mauro.a.castro at gmail.com, ...
Post-doctoral fellow, 2010-2014, Harvard University. Research Interests. Please see my website.. Selected Publications. Chubiz, L.M., Granger, B.R., Segrè, D., Harcombe, W.R., Species interactions differ in their genetic robustness, Frontiers Microbiol., (2015). Chubiz, L.M., Purswani, J., Carroll, S.M. and Marx, C.J., A novel pair of inducible expression vectors for use in Methylobacterium extorquens, BMC Research Notes, 6(186) (2013). Chubiz, L.M., Lee, M.C., Delaney, N.F. and Marx, C.J., FREQ-Seq: a rapid, cost-effective, sequencingbased method to determine allele frequencies directly from mixed populations. PLoS One, 7(10)(2012). Faculty of 1000 recommended by Drs. Andreas Wagner and Kathleen Sprouffske. Chubiz, L.M.,, Glekas, G.D. and Rao, C.V., Transcriptional cross talk within the mar-sox-rob regulon in Escherichia coli is limited to the rob and marRAB operons, Journal of Bacteriology, 194(18):4867-4875 (2012). Chubiz, L.M. and Rao, C.V., Role of the mar-sox-rob regulon in ...
Summary of Facts and Submissions. I. European patent application 78 300 596.0, filed on 6 November 1978 and published on 16 May 1979 with publication number 1929, was refused by the decision of the Examining Division of the European Patent Office dated 15 May 1985 and notified on 23 July 1985. The decision was based on Claims 1 to 12. The main Claims 1 and 9 were worded as follows: 1. A recombinant plasmid suited for transformation of a bacterial host comprising a homologous regulon and heterologous DNA, the heterologous DNA encoding a functional heterologous polypeptide or intermediate therefor, said homologous regulon being arranged with said heterologous DNA so as to control transcription and translation of said heterologous DNA encoding said functional heterologous polypeptide or intermediate therefor, whereby on translation of the transcription product of the heterologous DNA in a suitable bacterium, the resulting expression product is said functional polypeptide or intermediate therefor ...
Summary of Facts and Submissions. I. European patent application 78 300 596.0, filed on 6 November 1978 and published on 16 May 1979 with publication number 1929, was refused by the decision of the Examining Division of the European Patent Office dated 15 May 1985 and notified on 23 July 1985. The decision was based on Claims 1 to 12. The main Claims 1 and 9 were worded as follows: 1. A recombinant plasmid suited for transformation of a bacterial host comprising a homologous regulon and heterologous DNA, the heterologous DNA encoding a functional heterologous polypeptide or intermediate therefor, said homologous regulon being arranged with said heterologous DNA so as to control transcription and translation of said heterologous DNA encoding said functional heterologous polypeptide or intermediate therefor, whereby on translation of the transcription product of the heterologous DNA in a suitable bacterium, the resulting expression product is said functional polypeptide or intermediate therefor ...
protein,BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449,GlcT]: antitermination, via the [protein,BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449,GlcT]-dependent [SW,RNA switch] [PubMed,9765562], in [regulon,BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449,GlcT regulon ...
Grundy FJ, Henkin TM (1998) The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria. Mol Microbiol 30:737-49.[PMID:10094622 ...
Grundy FJ, Henkin TM (1998) The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria. Mol Microbiol 30:737-49.[PMID:10094622 ...
The cell envelope of bacteria is of pivotal importance for growth and survival, and hence it is often the target of antimicrobial compounds. One of the main components involved in CESRs are extracytoplasmic function (ECF) [sigma] factors. The genome of B. subtilis encodes for seven ECF [sigma] factors, [sigma]M, [sigma]W, [sigma]X, [sigma]Y, [sigma]V, [sigma]Z and [sigma]YlaC. Several studies have been conducted to understand the role that these ECF [sigma] factors play in CESR in B. subtilis, one of the challenges found is that they display significant redundancy within their regulons. In this study, we have performed an in depth analysis of one of the ECF [sigma] factors of B. subtilis, [sigma]V, which had been previously uncharacterized. We have described the regulon of [sigma]V, the role that it plays in lysozyme resistance, and provided evidence for a novel promoter element important for [sigma]V recognition. Additionally, we have studied the role that [sigma]M plays in moenomycin ...
Iron is an essential nutrient for nearly all living organisms (1). Transport systems for the uptake of iron and iron-chelation complexes are therefore critical for growth (64). In many bacteria, the ferric uptake regulator (Fur) protein coordinates the expression of iron uptake and homeostasis pathways in response to available iron (1). For example, in Escherichia coli K-12 the Fur regulon includes genes for the biosynthesis and transport of the catechol siderophore enterobactin, the uptake of various hydroxamate siderophores, and a substrate-inducible ferric citrate uptake system (25, 45). In addition, Fur represses a small RNA, RyhB, that in turn negatively regulates the expression of iron-rich enzymes such as succinate dehydrogenase, fumarase, and aconitase (36). This allows the production of these enzymes to be activated in response to available iron.. The production of low-molecular-weight Fe(III) chelators (siderophores) enables microorganisms to efficiently scavenge iron even in aerobic ...
Background The expression of genes in Corynebacterium glutamicum, a Gram-positive non-pathogenic bacterium used mainly for the industrial production of amino acids, is regulated by seven different sigma factors of RNA polymerase, including the stress-responsive ECF-sigma factor SigH. The sigH gene is located in a gene cluster together with the rshA gene, putatively encoding an anti-sigma factor. The aim of this study was to analyze the transcriptional regulation of the sigH and rshA gene cluster and the effects of RshA on the SigH regulon, in order to refine the model describing the role of SigH and RshA during stress response. Results Transcription analyses revealed that the sigH gene and rshA gene are cotranscribed from four sigH housekeeping promoters in C. glutamicum. In addition, a SigH-controlled rshA promoter was found to only drive the transcription of the rshA gene. To test the role of the putative anti-sigma factor gene rshA under normal growth conditions, a C. glutamicum rshA deletion ...
The soil living, Gram-positive bacterium Bacillus subtilis is frequently exposed to a wide variety of stress and starvation conditions in its natural environment. In order to survive under these environmental and energy stresses, the bacterium acquired a general stress response mechanism mediated by the alternative sigma factor, SigB. A wide-variety of stress conditions such as environmental stress conditions like ethanol stress, heat stress, oxidative stress, osmotic stress or limitation of glucose, oxygen, phosphate etc.; and low temperature growth induce this SigB-dependent general stress response. Though much is known about the mechanisms of activation of this general stress response, the conditions that induce the SigB regulon and its general functions, the definition of the structure of the SigB regulon is not completely clear. The SigB-dependent general stress regulon has previously been characterized by proteomic approaches as well as DNA-array based expression studies. Genome-wide ...
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Brief periods of heat stress of even a few days can have a detrimental effect on yield production worldwide causing devastating economical and sociological impacts. Here we report on the identification of a new heat-response regulon in plants controlled by the multiprotein bridging factor 1c (MBF1c) protein of Arabidopsis thaliana. Members of the highly conserved MBF1 protein family function as non DNA-binding transcriptional co-activators involved in regulating metabolic and development pathways in different organisms from yeast to humans. Nonetheless, our studies suggest that MBF1c from Arabidopsis functions as a transcriptional regulator which binds DNA and controls the expression of 36 different transcripts during heat stress, including the important transcriptional regulator DRE-binding protein 2A (DREB2A), two heat shock transcription factors (HSFs), and several zinc finger proteins. We further identify CTAGA as a putative response element for MBF1c, demonstrate that the DNA-binding domain ...
The stressosome complex regulates downstream effectors in response to environmental signals. In Bacillus subtilis, it activates the alternative sigma factor σ(B), leading to the upregulation of the general stress regulon. Herein, we characterize a stressosome-regulated biochemical pathway in Moorell …
FIG. 5. (A) Box plots of transcript expression Z scores for the genes in the σ32 heat shock regulon, oxidative stress-regulated genes (OxyR and SoxS oxidative stress regulons), and osmotically induced operons at 1 hour and 3 hours postinduction. The box plots include the Z scores for all genes detected in the respective functional category based upon EcoCyc (40) annotation and gene ontology classification. The local Z score generated from the SNOMAD analysis represents a change value weighted to account for the confidence in the data, and any gene with a Z score with an absolute value of ,1.96 was considered differentially expressed (9). The box plots show the values of the greatest down-regulation, lower quartile (Q1), median, upper quartile (Q3), and largest up-regulation observed for each profile for the regulon or gene group indicated. There were three expression profiles generated in this experiment, as follows: profile A, the 1- and 3-hour-postinduction transcript profile for the ...
Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR-CysK complex and transcriptional repression of the CymR regulon occurs. In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster dissociation of CymR from its DNA targets and the lifting of CymR-dependent repression ...
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Medizinische Universität Wien - Doktoratsprogram: Informationen zur Organisation, Studium und Lehre, Wissenschaft und Forschung.
Background: S. Typhi, a human-restricted Salmonella enterica serovar, causes a systemic intracellular infection in humans (typhoid fever). In comparison, S. Typhimurium causes gastroenteritis in humans, but causes a systemic typhoidal illness in mice. The PhoP regulon is a well studied two component (PhoP/Q) coordinately regulated network of genes whose expression is required for intracellular survival of S. enterica. Methodology/Principal Findings: Using high performance liquid chromatography mass spectrometry (HPLC-MS/MS), we examined the protein expression profiles of three sequenced S. enterica strains: S. Typhimurium LT2, S. Typhi CT18, and S. Typhi Ty2 in PhoP-inducing and non-inducing conditions in vitro and compared these results to profiles of \(phoP^−/Q^−\) mutants derived from S. Typhimurium LT2 and S. Typhi Ty2. Our analysis identified 53 proteins in S. Typhimurium LT2 and 56 proteins in S. Typhi that were regulated in a PhoP-dependent manner. As expected, many proteins ...
The bacterial cell envelope is the first and major line of defence against threats from the environment. It is an essential and vulnerable structure that gives the cell its shape and counteracts the high internal osmotic pressure. It also provides an important sensory interface and molecular sieve, mediating both information flow and controlled transport of solutes. The cell envelope is also the target for numerous antibiotics. Therefore, the monitoring and maintaining of cell envelope integrity in the presence of envelope perturbating agents and conditions is crucial for survival. In Bacillus subtilis a complex regulatory network, consisting of 7 signal transducing systems, orchestrates the cell envelope stress response. Two forms of regulatory systems can be found: ECF-sigma factors and two component systems (TCS). One of these TCS is the LiaRS system that responds to cell wall antibiotics that interfere with the undecaprenol cycle and to perturbation of the cytoplasmic me! mbrane. It is ...
The general stress response comprises approximately 200 genes and is driven by the alternative sigma factor SigB. Besides the process of sporulation with approximately 500 involved gene products under initial control of Spo0A are the two most significant and extensive cellular responses that can be observed in B. subtilis. The general stress response provides vegetative growing as well as non-growing and non-sporulating cells with a comprehensive cross-protective and preventive multiple stress resistance to various hostile environmental conditions. In contrast, the endospore is the most resistant but also dormant cell type produced by B. subtilis. The scope of this study was the identification of regulatory cascades driven by the general stress response sigma factor SigB to further elucidate the structure and function of the general stress regulon itself and to uncover potential intersections between the SigB response and other major developmental programs in the regulatory network of B. ...
Fingerprint Dive into the research topics of Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set. Together they form a unique fingerprint. ...
This chapter summarizes studies that led to identification and characterization of the Cpx envelope stress response and highlights recent work that hints at a diverse range of Cpx-influenced cellular phenotypes. Silverman and colleagues provided the kernels for our current understanding of the Cpx envelope stress response. The Cpx envelope stress response is regulated by a typical two-component regulatory system. In addition to functioning as an activator and a repressor, a new role for CpxR~P in transcription has recently been put forth, that of potentiator. Researchers have shown by microarray analysis of biofilm and planktonic populations of cells that the Cpx-regulated genes cpxP and spy were highly up-regulated in biofilms and that cpxP and cpxR mutants formed biofilms with reduced mass and substrate coverage. It has been shown that CpxR homologues in Legionella pneumophila and Yersinia enterocolitica are likely involved in transcription of the icm-dot genes encoding the type IV secretion system
During the production of wine and beer, the yeast Saccharomyces cerevisiae can encounter an environment that is deficient in zinc, resulting in a sluggish or a stuck ferment. It has been shown that the Zap1p-transcription factor induces the expression of a regulon in response to zinc deficiency; however, it was evident that a separate regulon was also activated during zinc deficiency in a Zap1p-independent manner. This study discovered the Msn2p and Msn4p (Msn2/4p) transcriptional activator proteins to be an additional control mechanism inducing the stress response during zinc deficiency. Promoter sequence analysis identified the stress response element (STRE) motif, recognized by Msn2/4p, and was significantly enriched in the promoters of genes induced by zinc deficiency. An investigation using genome-wide analyses revealed a distinct regulon consisting of STREcontaining genes whose zinc-responsive expression was abolished in an msn2 msn4 double mutant. An STRE-driven lacZ reporter ...
The exoenzyme S regulon is a set of coordinately regulated virulence genes of Pseudomonas aeruginosa. Proteins encoded by the regulon include a type III secretion and translocation apparatus, regulators of gene expression, and effector proteins. The effector proteins include two enzymes with ADP-ribosyltransferase activity (ExoS and ExoT) and an acute cytotoxin (ExoU). In this study, we identified ExoY as a fourth effector protein of the regulon. ExoY is homologous to the extracellular adenylate cyclases of Bordetella pertussis (CyaA) and Bacillus anthracis (EF). The homology among the three adenylate cyclases is limited to two short regions, one of which possesses an ATP-binding motif. In assays for adenylate cyclase activity, recombinant ExoY (rExoY) catalyzed the formation of cAMP with a specific activity similar to the basal activity of CyaA. In contrast to CyaA and EF, rExoY activity was not stimulated or activated by calmodulin. A 500-fold stimulation of activity was detected following the ...
Wells, Hannah (2015) Further characterisation of the envelope stress responses of Salmonella Typhimurium. Doctoral thesis, University of East Anglia. ...
is a major cause of pharyngitis in humans and encodes several fibronectin-binding proteins. lines and tonsillar epithelial cells (5, 15). The regulon encodes one or more M-like proteins, Velcade which vary functionally in their abilities to bind immunoglobulins, Fn, fibrinogen, and albumin (7, 9). Protein F1 (PrtF1/SfbI), which is not part of the regulon, also binds Fn and promotes adhesion and internalization by epithelial cells (11, 17, 24, 26). M protein and PrtF1/SfbI are genetically unlinked, and their expression is differentially regulated by oxygen and carbon dioxide, respectively (10). M proteins expression enables to withstand phagocytosis by two systems. M protein-expressing bacterias bind element H, a regulator from the go with program, which inhibits C3b deposition (9). M proteins can bind fibrinogen, which inhibits the choice go with pathway (12). The opsonization of with neutralizing antibodies against M proteins or other surface area proteins enhances C3 fixation and following ...
The σE-dependent extracytoplasmic stress response.σE is held at the membrane by the antisigma factor RseA. RseB binds to the periplasmic domain of RseA and pr
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Phosphate plays an essential role in nearly all metabolic, catabolic, and signaling events within virtually all living organisms. As such, the trafficking of phosphate anions is an immensely important function, yet the specific mechanism utilized to transport inorganic phosphate molecules across a lipid bilayer remains unclear. The Gram negative bacterial cell utilizes a double membrane system and employs a plethora of channels that can be constitutive or specifically expressed in its outer membrane (OM) in response to various environmental conditions. Most bacteria, including Pseudomonas react to environmental conditions of low phosphate by turning on a gene regulon leading to the expression of a number of phosphate trafficking proteins (Figure 2). This regulation is mediated by regulatory proteins anchored in the inner membrane (PhoR), that sense phosphate levels through an as yet uncharacterized interaction with the inner membrane transport complex.. ...
Ahuja N, Korkin D, Chaba R, Cezairliyan BO, Sauer RT, Kim KK, Gross CA. Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy. J. Biol. Chem. 2009 Feb 20; 284(8):5403-13 ...
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Die CT-Angiographie (CTA) ist in den aktualisierten Richtlinien zur Feststellung des irreversiblen Hirnfunktionsausfalles als ergänzendes apparatives
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150 µg desogestrel (AU, EU, RU, UK: Marvelon, BR: Microdiol, US: Desogen, MSD; US: Ortho-Cept, Ortho-McNeil; RU: Regulon, ...
lac operon Weickert, M. J.; Adhya, S. (October 1993). "The galactose regulon of Escherichia coli". Molecular Microbiology. 10 ( ...
GalR is a transcriptional regulator of the galactose regulon composed of genes encoding enzymes for transport and metabolism of ... Weickert MJ, Adhya S (October 1993). "The galactose regulon of Escherichia coli". Molecular Microbiology. 10 (2): 245-51. doi: ...
Brown, S.; Cole, M. & Erives, A.J. (2008). "Evolution of the holozoan ribosome biogenesis regulon". BMC Genomics. 9: 442. doi: ...
Weickert MJ, Adhya S (1993). "The galactose regulon of Escherichia coli" (PDF). Mol. Microbiol. 10 (2): 245-51. doi:10.1111/j. ...
Nif regulon (Klebsiella pneumoniae) Spaink, Herman P (1998). The Rhizobiaceae: Molecular Biology of Model Plant-Associated ...
SoxRS regulon genes can be regulated by additional factors. At least three known genes including xthA and katE are regulated by ... Regulation of the soxRS regulon occurs by a two-stage process: the SoxR protein is first converted to an oxidized form that ... The oxidized form of this factor specifically binds to the promoters of constituent genes of OxyR regulon, including katG ( ... The transcriptional factor OxyR regulates the expression of OxyR regulon. H2O2 oxidizes the transcriptional factor by forming ...
Bhat PJ (2 March 2008). Galactose Regulon of Yeast: From Genetics to Systems Biology. Springer Science & Business Media. ISBN ...
"A miR-19 regulon that controls NF-iB signaling". Nucleic Acids Research. 40 (16): 8048-8058. doi:10.1093/nar/gks521. PMC ...
Keene, J. D. (January 2007). "Biological Clocks and the Coordination Theory of RNA Operons and Regulons". Cold Spring Harbor ... He formalized the posttranscriptional operon and regulon (PTRO) model to describe global gene regulation, and proposed the RNA ... By 2007, Keene proposed the RNA regulon hypothesis, "that mRNAs encoded by functionally related genes may be coordinately ... Simone, Laura E; Keene, Jack D (February 2013). "Mechanisms coordinating ELAV/Hu mRNA regulons". Current Opinion in Genetics & ...
CS1 maint: discouraged parameter (link) Cozzarelli, Nicholas Robert (1996). The L-L-Glycerophosphate Regulon in Escherichia ...
Hondorp ER, McIver KS (December 2007). "The Mga virulence regulon: infection where the grass is greener". Molecular ... the missing links between the CovR regulatory cascade and the Mga regulon". Molecular Microbiology. 66 (6): 1506-22. doi: ...
"Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducens". BMC Genomics. 10: 331. doi:10.1186/1471-2164-10-331. PMC ...
Pelechano, V; Jimeno-González, S; Rodríguez-Gil, A; García-Martínez, J; Pérez-Ortín, JE; Chávez, S (Aug 2009). "Regulon- ...
Coornaert A, Lu A, Mandin P, Springer M, Gottesman S, Guillier M (April 2010). "MicA sRNA links the PhoP regulon to cell ... "Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins". Journal ...
Keene JD (2007). "RNA regulons: coordination of post-transcriptional events". Nature Reviews Genetics. 8 (7): 533-543. doi: ...
She presented a paper entitled The Xanthomonas axonopodis citri PHO regulon. Most media relating to Taschner can be found on ...
This single [repressor controls the transcription of the whole fad regulon. Binding of fadR is specifically inhibited by long ...
P. Chen, D. I. Andersson & J. R. Roth (September 1994). "The control region of the pdu/cob regulon in Salmonella typhimurium". ... control the cobalamin/propanediol regulon of Salmonella typhimurium". Journal of Bacteriology. 175 (22): 7200-7208. doi:10.1128 ...
Bacterial small RNA Shimizu, T; Yaguchi, H; Ohtani, K; Banu, S; Hayashi, H (Jan 2002). "Clostridial VirR/VirS regulon involves ... Shimizu, T; Yaguchi, H; Ohtani, K; Banu, S; Hayashi, H (Jan 2002). "Clostridial VirR/VirS regulon involves a regulatory RNA ... "Identification of a two-component VirR/VirS regulon in Clostridium perfringens". Anaerobe. 16 (3): 258-264. doi:10.1016/j. ... "Identification of a two-component VirR/VirS regulon in Clostridium perfringens". Anaerobe. 16 (3): 258-264. doi:10.1016/j. ...
... s are related to regulons, stimulons and modulons; whereas operons contain a set of genes regulated by the same operator ... regulons contain a set of genes under regulation by a single regulatory protein, and stimulons contain a set of genes under ...
Silakowski, B.; Kunze, B.; Nordsiek, G.; Blöcker, H.; Höfle, G.; Müller, R. (2000). "The myxochelin iron transport regulon of ...
Png, K. J.; Halberg, N.; Yoshida, M.; Tavazoie, S. F. (2011). "A microRNA regulon that mediates endothelial recruitment and ...
Qin, N (2007). "Analysis of LuxR regulon gene expression during quorum sensing in Vibrio fischeri". J Bacteriol. 189 (11): 4127 ...
"Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis". PLOS ONE. 6 (12): e29002. doi: ...
The genes required for the synthesis of cysteine are coded for on the cys regulon. The integration of sulfur is positively ... CysB functions by binding to DNA half sites on the cys regulon. These half sites differ in quantity and arrangement depending ... Without the accessory half sites the regulon cannot be transcribed and cysteine will not be produced. It is believed that the ... The RNA polymerase will then transcribe the cys regulon and cysteine will be produced. Further regulation is required for this ...
Smith, J.N.; Ahmer, B.M. (February 2003). "Detection of other microbial species by Salmonella: expression of the SdiA regulon ...
Mey AR, Craig SA, Payne SM (September 2005). "Characterization of Vibrio cholerae RyhB: the RyhB regulon and role of ryhB in ... "Characterization of the small untranslated RNA RyhB and its regulon in Vibrio cholerae". Journal of Bacteriology. 187 (12): ...
... coli Zur regulon". PLOS Biology. 12 (11): e1001987. doi:10.1371/journal.pbio.1001987. PMC 4219657. PMID 25369000. Blindauer CA ...
Regulons can sometimes be pathogenicity islands. The Ada regulon in E. coli is a well-characterized example of a group of genes ... Commonly studied regulons in bacteria are those involved in response to stress such as heat shock. The heat shock response in E ... In molecular genetics, a regulon is a group of genes that are regulated as a unit, generally controlled by the same regulatory ... Regulons involving virulence factors in pathogenic bacteria are of particular research interest; an often-studied example is ...
In bacterial genetics, the mal regulon is a regulon - or group of genes under common regulation - associated with the ... "Maltose-Dependent Transcriptional Regulation of the mal Regulon by MalR in Streptococcus pneumoniae". PLOS ONE. 10 (6): ...
RNA regulons posted by amnestic @ 8/23/2007 10:59:00 PM RNA regulons ... The idea of RNA regulons is nice, but how much can you predict knowing that your gene of interest is part of one? RNAs ... Any process better controlled faster or farther away from the nucleus could use an RNA regulon. One notable case is that of the ... One of my favorite recent ideas wondering through the literature is that of an RNA regulon or post-transcriptional operon. ...
Galactose Regulon of Yeast: From Genetics to Systems Biology, 9781281241863, available at Book Depository with free delivery ...
ToxR regulon of Vibrio cholerae and its expression in vibrios shed by cholera patients. James Bina, Jun Zhu, Michelle Dziejman ... In this report, we took advantage of our knowledge of the ToxR regulon to test the utility of a gDNA reference in microarray ... Virulence gene expression in V. cholerae is subject to the hierarchy of the ToxR regulon, where both ToxT-dependent and ToxT- ... We chose the well-characterized ToxR regulon of V. cholerae as an experimental system to address these questions. Our studies ...
This CRE regulon contains 25 genes in 13 transcription units, only about half of which have been previously associated with ... A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae.. Redfield RJ1, Cameron AD, ... Competence-induced transcription of genes in the CRE regulon is strongly dependent on cAMP, consistent with the known role of ... This analysis confirmed the existence of a postulated competence regulon, characterized by a promoter-associated 22 bp ...
A Regulon filmtabletta terhess g megel z s re szolg l , szint ... Milyen t pus gy gyszer a Regulon filmtabletta s milyen betegs ... Ha m s kombin lt hormon lis fogamz sg tl r l v lt Regulon tablett ra. A Regulon tabletta szed s t elkezdheti azon a napon, ... 2. Tudnival k a Regulon filmtabletta szed se el tt. ltal nos megjegyz sek. A Regulon filmtabletta alkalmaz s nak megkezd se el ... 1. Milyen t pus gy gyszer a Regulon filmtabletta s milyen betegs gek eset n alkalmazhat ?. A Regulon filmtabletta terhess g ...
ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.. About ASM , Contact Us , Press Room. ASM is a member of. ...
Characteristic induction profile of the σM, σW, σB, σY, and Spx regulons. The induction profiles (log scale) for the clusters ... The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.. Eiamphungporn W1, Helmann JD. ... y-axis). Genes induced by vancomycin were assigned to regulons as indicated in the inset. "sigM(new)" indicates those genes ... Cell envelope stress responses also include regulons controlled by sigma(W), sigma(B) and several two-component regulatory ...
... which has significantly altered the composition of bacterial regulons. We now demonstrate that the evolution of the regulons ... Transcription factor function and promoter architecture govern the evolution of bacterial regulons. J. Christian Perez and ... Promoter Strength Properties of the Complete Sigma E Regulon of Escherichia coli and Salmonella enterica ... Transcription factor function and promoter architecture govern the evolution of bacterial regulons ...
The BvgAS Regulon of Bordetella pertussis. Kyung Moon, Richard P. Bonocora, David D. Kim, Qing Chen, Joseph T. Wade, Scott ... The BvgAS Regulon of Bordetella pertussis. Kyung Moon, Richard P. Bonocora, David D. Kim, Qing Chen, Joseph T. Wade, Scott ... The BvgAS Regulon of Bordetella pertussis. Kyung Moon, Richard P. Bonocora, David D. Kim, Qing Chen, Joseph T. Wade, Scott ... Species- and strain-specific control of a complex, flexible regulon by Bordetella BvgAS. J Bacteriol188:1775-1785. doi:10.1128/ ...
Transcriptional analysis of the flagellar regulon of Salmonella typhimurium.. K Kutsukake, Y Ohya, T Iino ... Transcriptional analysis of the flagellar regulon of Salmonella typhimurium. Message Subject (Your Name) has forwarded a page ... These results are compared with the cascade model of the flagellar regulon of Escherichia coli proposed previously (Y. Komeda, ...
... suggesting that the regulon might be as large as that of the well-characterized homologous regulon of B. subtilis (reviewed in ... Microarray-Based Analysis of the Staphylococcus aureus σB Regulon. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal ... Microarray-Based Analysis of the Staphylococcus aureus σB Regulon. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal ... Microarray-Based Analysis of the Staphylococcus aureus σB Regulon. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal ...
We call this group of genes the "homocysteine regulon".. Keywords. Aspergillus nidulans Transsulfuration Cystathionine β- ...
... we asked if exposure to maltose reduces MalT regulon transcription. First, we monitored MalT regulon expression of an ompR malT ... A. Hartmann and W. Boos, "Mutations in phoB, the positive gene activator of the pho regulon in Escherichia coli, affect the ... O. Raibaud and E. Richet, "Maltotriose is the inducer of the maltose regulon of Escherichia coli," Journal of Bacteriology, vol ... C. Chapon, "Role of the catabolite activator protein in the expression of the maltose regulon of Escherichia coli," Annales de ...
This regulon is distinct from the set of ribosomal protein genes, and the promoters of the expanded RRB gene set are highly ... The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes Yeast. 2006 Mar;23(4):293-306. doi: ... Since a similar pattern of organization and gene regulation can be recognized in C. albicans, the RRB regulon appears to be a ... we have extended that study and now suggest that the ribosomal and rRNA biosynthesis regulon contains over 200 genes. ...
We have used DNA microarrays to define the Fur regulon and the iron starvation stimulon. We identify 20 operons (containing 39 ... In addition to the Fur regulon, iron starvation induces members of the PerR regulon and leads to reduced expression of ... Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon Mol Microbiol. 2002 Sep;45(6):1613-29. ... We have used DNA microarrays to define the Fur regulon and the iron starvation stimulon. We identify 20 operons (containing 39 ...
We propose that the different roles of the S. sanguinis, S. pneumoniae, and S. mutans com regulons in virulence can be ... These results suggest that the com regulon is not required for S. sanguinis infective endocarditis virulence in this model. ... Previous studies of Streptococcus pneumoniae and Streptococcus mutans have identified functions for the ,100-gene com regulon ... Regulons Is the Subject Area "Regulons" applicable to this article? Yes. No. ...
Browse our Mycobacterium Tuberculosis RV3134 Dormancy Regulon product catalog backed by our Guarantee+. ... Mycobacterium Tuberculosis RV3134 Dormancy Regulon products available through Novus Biologicals. ...
This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB ... Phosphate regulon transcriptional regulatory protein PhoBAdd BLAST. 229. Amino acid modifications. Feature key. Position(s). ... sp,P0AFJ5,PHOB_ECOLI Phosphate regulon transcriptional regulatory protein PhoB OS=Escherichia coli (strain K12) GN=phoB PE=1 SV ... This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB ...
Regulons, and Cascades: Control of Bacterial Gene Expression (Society for General Microbiology Symposia 61) by From WHSmith ... Signals, Switches, Regulons, and Cascades: Control of Bacterial Gene Expression (Society for General Microbiology Symposia 61) ... Quorum sensing in Gram-negative bacteria: global regulons controlled by cell-density-dependent chemical signalling N. A. ...
hnRNPs Interacting with mRNA Localization Motifs Define Axonal RNA Regulons. Seung Joon Lee, Juan A. Oses-Prieto, Riki ... hnRNPs Interacting with mRNA Localization Motifs Define Axonal RNA Regulons. Seung Joon Lee, Juan A. Oses-Prieto, Riki ... hnRNPs Interacting with mRNA Localization Motifs Define Axonal RNA Regulons. Seung Joon Lee, View ORCID ProfileJuan A. Oses- ... hnRNPs Interacting with mRNA Localization Motifs Define Axonal RNA Regulons Message Subject (Your Name) has sent you a message ...
These results demonstrated that a subgenomic oligonucleotide microarray is a powerful tool for defining the SalA regulon and ... To define the SalA regulon, the spotted oligonucleotide microarray was constructed using gene-specific 70-mer oligonucleotides ...
The NodD3 regulon of S. meliloti appeared to be much larger than the NodD1 regulon since hundreds of genes were induced or ... The NodD1 regulon of S. meliloti is thus much less extended than that of the broad-host-range rhizobium NGR234, in which at ... To our surprise, at 24 hpi the NodD1 regulon of S. meliloti was restricted to a few nod genes in contrast to what happens in ... The NodD1 regulon of Sinorhizobium meliloti was determined through the analysis of the S. meliloti transcriptome in response to ...
Large-scale regulon inference using bioinformatics revealed remarkable variations in transcriptional regulators for L-Rha ... Large-scale regulon inference using bioinformatics revealed remarkable variations in transcriptional regulators for L-Rha ... A comparative genomics approach was used to reconstruct the L-Rha catabolic pathways and transcriptional regulons in the phyla ... A comparative genomics approach was used to reconstruct the L-Rha catabolic pathways and transcriptional regulons in the phyla ...
Aspergillus niger - CreA - D-xylose - Dynamic modeling - Parameter estimation - XlnR regulon Abstract. Background: ... In this work we analyse and model the transcription dynamics in the XlnR regulon from time-course data of the messenger RNA ... We show that perturbing the XlnR regulon with Xyl in low and high concentrations results in different expression levels and ... Quantitative modeling and analytic assessment of the transcription dynamics of the XlnR regulon in Aspergillus niger. ...
The PhoP regulon is a well studied two component (PhoP/Q) coordinately regulated network of genes whose expression is required ... Comparative proteomic analysis of the PhoP regulon in Salmonella enterica Serovar Typhi Versus Typhimurium. PLoS ONE 4(9): ... Conclusions/Significance: This study is the first protein expression study of the PhoP virulence associated regulon using ... Comparative Proteomic Analysis of the PhoP Regulon in Salmonella enterica Serovar Typhi Versus Typhimurium. ...
Characterization of the AggR Regulon in Enteroaggregative Escherichia coli. Nicholas Morin, Araceli E. Santiago, Robert K. ... Characterization of the AggR Regulon in Enteroaggregative Escherichia coli. Nicholas Morin, Araceli E. Santiago, Robert K. ... Characterization of the AggR Regulon in Enteroaggregative Escherichia coli. Nicholas Morin, Araceli E. Santiago, Robert K. ... When the AggR regulon is activated, the ORF4 product hypothetically provides supplemental IDI activity to meet the demand of ...
Genomewide Expression Profile Analysis of the Candida glabrata Pdr1 Regulon. Kelly E. Caudle, Katherine S. Barker, Nathan P. ... Genomewide Expression Profile Analysis of the Candida glabrata Pdr1 Regulon. Kelly E. Caudle, Katherine S. Barker, Nathan P. ... Genomewide Expression Profile Analysis of the Candida glabrata Pdr1 Regulon. Kelly E. Caudle, Katherine S. Barker, Nathan P. ... Genomewide Expression Profile Analysis of the Candida glabrata Pdr1 Regulon Message Subject (Your Name) has forwarded a page to ...
Next we analyzed how many of the 36 salt-responsive SAGs of the ANAC092 regulon are affected by H2O2 and found that 14 of them ... Salt-triggered expression of the ANAC092-dependent senescence regulon in Arabidopsis thaliana. Salma Balazadeh,1 Anhui Wu,2 and ... Further work is clearly needed to unravel the precise make-up of the ANAC092 senescence regulon and its integration into other ... Table 1), representing ~40% of the ANAC092 salt-dependent senescence regulon. However, five genes were repressed by H2O2- ...
  • These results are compared with the cascade model of the flagellar regulon of Escherichia coli proposed previously (Y. Komeda, J. Bacteriol. (asm.org)
  • By incorporating a multi-scale approach that accounts for mechanistic properties of promoters and maps those findings to a genome-scale metabolic-regulatory network, the Escherichia coli Crp regulon is revealed, enabling systems analytics to be applied to delineate its governing role in regulating cellular carbon flow. (omicsdi.org)
  • MTB Recombinant Protein of Dormancy Regulon antibody LS-C52009 is an unconjugated mouse monoclonal antibody to mycobacterium tuberculosis MTB Recombinant Protein of Dormancy Regulon. (lsbio.com)
  • Elucidating the Regulon of a Fur-like Protein in Mycobacterium avium subsp. (unl.edu)
  • the genes contained within a regulon are usually organized into more than one operon at disparate locations on the chromosome. (wikipedia.org)
  • One of my favorite recent ideas wondering through the literature is that of an RNA regulon or post-transcriptional operon. (gnxp.com)
  • Regulon vs Operon - What's the difference? (wikidiff.com)
  • Operon is a see also of regulon . (wikidiff.com)
  • The genes for these virulence determinants belong to a network of genes (the ToxR regulon) whose expression is modulated by transcriptional regulators encoded by the toxRS , tcpPH , and toxT genes. (pnas.org)
  • Thus, in vitro analysis of the ToxR regulon has demonstrated the existence of an extremely complex regulatory network controlling the expression of the TCP and CT virulence genes. (pnas.org)
  • The data presented here suggest that the σ B of S. aureus controls a large regulon and is an important modulator of virulence gene expression that is likely to act conversely to RNAIII, the effector molecule of the agr locus. (asm.org)
  • 100-gene com regulon in addition to DNA uptake, including virulence. (plos.org)
  • These results suggest that the com regulon is not required for S. sanguinis infective endocarditis virulence in this model. (plos.org)
  • We propose that the different roles of the S. sanguinis , S. pneumoniae , and S. mutans com regulons in virulence can be understood in relation to the pathogenic mechanisms employed by each species. (plos.org)
  • To define the SalA regulon, the spotted oligonucleotide microarray was constructed using gene-specific 70-mer oligonucleotides of all open reading frames (ORFs) predicted in the syringomycin (syr) and syringopeptin (syp) gene clusters along with representative genes important to bacterial virulence, growth, and survival. (apsnet.org)
  • Conclusions/Significance: This study is the first protein expression study of the PhoP virulence associated regulon using strains of Salmonella mutant in PhoP, has identified three Typhi-unique proteins (CdtB, HlyE and STY1499) that are not present in the genome of the wide host-range Typhimurium, and includes the first protein expression profiling of a live attenuated bacterial vaccine studied in humans (Ty800). (harvard.edu)
  • Expression of virulence-associated determinants is strictly and coordinately controlled by a set of transcriptional regulators and complex signal transduction cascades are responsible for sensing and integrating the environmental information into the virulence regulons [ 1 - 4 ]. (prolekare.cz)
  • Results of real-time PCR and biochemical assays demonstrated that betulin suppresses the expression of ropB core regulon, sagA and dltA, which correspondingly affects SpeB production, hemolysis and cell surface hydrophobicity for the observed impairment in virulence and biofilm formation. (ovid.com)
  • Genes comprising the σ B regulon encode solute transporters, novel cell-wall proteins, universal stress proteins, transcriptional regulators and include those involved in osmoregulation, carbon metabolism, ribosome- and envelope-function, as well as virulence and niche-specific survival genes such as those involved in bile resistance and exclusion. (biomedcentral.com)
  • Transcriptional analysis of the flagellar regulon of Salmonella typhimurium. (asm.org)
  • The genes specifying motility and flagellar functions are coordinately regulated and form a complex regulon, the flagellar regulon. (nih.gov)
  • A). At the top of the network, FliZ coordinates the expression of flagellum-driven motility through a feedback effect on the expression of flhD (the flagellar regulon) and genes encoding non-flagellar proteins involved in the lifecycle of the bacterium and its interaction with invertebrates [19]. (nih.gov)
  • The genes of the flagellar system, regulator and structural genes, are structured in hierarchical levels described as the flagellar regulon. (rki.de)
  • For L. pneumophila it was shown that the expression of genes of the flagellar regulon is modulated by the actual growth phase and temperature. (rki.de)
  • In the following review, the structure of the flagellar regulon of L. pneumophila is discussed and compared to other flagellar systems of different Legionella species. (rki.de)
  • Here we provide additional characterization of the AggR regulon. (asm.org)
  • Characterization of the Vibrio cholerae ToxR regulon: identification of novel genes involved in intestinal colonization. (asm.org)
  • Keywords: Transcriptional response to 3 oxidants (H2O2, menadione and cumene hydroperoxide) and characterization of the perR regulon (comparison of the transciptomes from the wild-type and perR mutant). (omicsdi.org)
  • A modulon is a set of regulons or operons that are collectively regulated in response to changes in overall conditions or stresses, but may be under the control of different or overlapping regulatory molecules. (wikipedia.org)
  • This CRE regulon contains 25 genes in 13 transcription units, only about half of which have been previously associated with competence. (nih.gov)
  • Competence-induced transcription of genes in the CRE regulon is strongly dependent on cAMP, consistent with the known role of catabolite regulation in competence. (nih.gov)
  • The essential competence gene sxy is induced early in competence development and is required for competence-induced transcription of CRE-regulon genes but not other CRP-regulated genes, suggesting that Sxy may act as an accessory factor directing CRP to CRE sites. (nih.gov)
  • sigma(M) also contributes to the expression of the Spx transcription factor and thereby indirectly regulates genes of the Spx regulon. (nih.gov)
  • We posit that these changes allow bacterial transcription factors to incorporate newly acquired genes into ancestral regulatory circuits and yet retain control of the core members of a regulon. (pnas.org)
  • Here, we have used transcriptome sequencing (RNA-seq) and reverse transcription-quantitative PCR (RT-qPCR) to define the BvgAS-dependent regulon of B. pertussis Tohama I. Our analyses reveal more than 550 BvgA-regulated genes, of which 353 are newly identified. (asm.org)
  • In this work we analyse and model the transcription dynamics in the XlnR regulon from time-course data of the messenger RNA levels for some XlnR target genes, obtained by reverse transcription quantitative PCR (RT-qPCR). (wur.nl)
  • We show that perturbing the XlnR regulon with Xyl in low and high concentrations results in different expression levels and transcription dynamics of the target genes. (wur.nl)
  • Genome-Wide Chromatin Immunoprecipitation Sequencing Analysis Shows that WhiB Is a Transcription Factor That Cocontrols Its Regulon with WhiA To Initiate Developmental Cell Division in Streptomyces. (sigmaaldrich.com)
  • Further, we show that WhiB does not act independently but rather coregulates its regulon of sporulation genes with a partner transcription factor, WhiA. (sigmaaldrich.com)
  • When cells were treated with beta-lactams, BlaI was released from its operator sites causing derepression of the regulon and upregulation of ATP synthase transcription. (pasteur.fr)
  • Oligonucleotide Microarray Analysis of the SalA Regulon Controlling Phytotoxin Production by Pseudomonas syringae pv. (apsnet.org)
  • Expression of the Pho regulon negatively regulates biofilm formation by Pseudomonas aureofaciens PA147-2. (mendeley.com)
  • Bioinformatics and transcriptomics analyses of the HrpL regulon in Pseudomonas savastanoi pathovars of woody host. (uma.es)
  • This conservation of RBPs is hypothetically due to the need for coordination of global gene expression by RNA regulons that coordinate ribonomic regulators of regulators. (utoronto.ca)
  • We therefore investigated the ArcA regulon in aerobically growing S. Typhimurium before and after exposure to H 2 O 2 by querying gene expression and other physiological changes in wild type and ΔarcA strains.Results: In the ΔarcA strain, expression of 292 genes showed direct or indirect regulation by ArcA in response to H 2 O 2 , of which 141were also regulated in aerobiosis, but in the opposite direction. (unab.cl)
  • In molecular genetics, a regulon is a group of genes that are regulated as a unit, generally controlled by the same regulatory gene that expresses a protein acting as a repressor or activator. (wikipedia.org)
  • The Ada regulon in E. coli is a well-characterized example of a group of genes involved in the adaptive response form of DNA repair. (wikipedia.org)
  • In bacterial genetics, the mal regulon is a regulon - or group of genes under common regulation - associated with the catabolism of maltose and maltodextrins. (wikipedia.org)
  • Since a similar pattern of organization and gene regulation can be recognized in C. albicans, the RRB regulon appears to be a conserved, extensive, and metabolically important group of genes. (nih.gov)
  • Commonly studied regulons in bacteria are those involved in response to stress such as heat shock. (wikipedia.org)
  • It is presently unclear how the evolution of gene regulatory circuits has proceeded in bacteria, given the rampant effects of horizontal gene transfer, which has significantly altered the composition of bacterial regulons. (pnas.org)
  • In order to determine the extent of the NodD1 regulon in Sinorhizobium meliloti , we analyzed the transcriptome of bacteria in response (i) to the flavone luteolin, an activator of NodD1, and (ii) to the overexpression of NodD1 (Tables 1 and 2 ). (asm.org)
  • Large-scale regulon inference using bioinformatics revealed remarkable variations in transcriptional regulators for L-Rha utilization genes among bacteria. (frontiersin.org)
  • Given that these bacteria occupy different ecological niches it would be interesting to examine the extent of conservation of the σ B regulons amongst these bacteria. (biomedcentral.com)
  • Therefore, we conclude that the PhoP/Q regulon enables S . Typhimurium to adapt to intramacrophage stresses other than phagolysosomal fusion. (microbiologyresearch.org)
  • The identified genes belonging to the S. Typhimurium σ E -regulon encode proteins involved in primary metabolism, DNA repair systems and outer-membrane biogenesis, and regulatory proteins, periplasmic proteases and folding factors, proposed lipoproteins, and inner- and outer-membrane proteins with unknown functions. (microbiologyresearch.org)
  • In absence of ArcA under aerobic conditions, H 2 O 2 exposure resulted in lower levels of glutathione reductase activity, leading to a decreased GSH (reduced glutathione)/GSSG (oxidized glutathione) ratio.Conclusion: The ArcA regulon was defined in 2 conditions, aerobic growth and the combination of peroxide treatment and aerobic growth in S. Typhimurium. (unab.cl)
  • This analysis confirmed the existence of a postulated competence regulon, characterized by a promoter-associated 22 bp competence regulatory element (CRE) closely related to the cAMP receptor protein (CRP) binding consensus. (nih.gov)
  • Predicted promoter changes suggesting differential transcriptional control of a common gene pool predominate over evidence of indels or horizontal gene transfer for explaining SigB regulon divergence. (biomedcentral.com)
  • Identification of a dehydration and ABA-responsive promoter regulon and isolation of. (deepdyve.com)
  • The genes of a regulon share a common regulatory element binding site or promoter. (wikidiff.com)
  • For example, it identified the bacteriorhodopsin regulon, assigned additional genes to this regulon with apparently unrelated function, and detected its known promoter motif. (springer.com)
  • The NodD1 regulon of Sinorhizobium meliloti was determined through the analysis of the S. meliloti transcriptome in response to the plant flavone luteolin and the overexpression of nodD1 . (asm.org)
  • A more complete picture of the σ 54 regulon was achieved by combining the transcriptome data with an in silico search for potential σ 54 -dependent promoters, using a position weight matrix approach. (biomedcentral.com)
  • Together, these results show that nitrogen starvation causes intense changes in the X. fastidiosa transcriptome and some of these differentially expressed genes belong to the σ 54 regulon. (biomedcentral.com)
  • The AmtR regulon was characterized in this study by a combination of bioinformatics, transcriptome and proteome analyses. (uni-bielefeld.de)
  • We report the use of Illumina based transcriptome sequencing to determine the RamA Regulon in K. pneumoniae. (omicsdi.org)
  • In this thesis, we first investigated the regulon of the alternative sigma factor E by employing next-generation sequencing of both the genome and the transcriptome of N. meningitidis wildtype and an overexpression mutant. (uva.nl)
  • an often-studied example is the phosphate regulon in E. coli, which couples phosphate homeostasis to pathogenicity through a two-component system. (wikipedia.org)
  • This protein is a positive regulator for the phosphate regulon. (uniprot.org)
  • Single-cell analysis revealed the bimodal expression of six identified markers of the FliZ regulon during exponential growth of the bacterial population. (nih.gov)
  • Identification of the MBF1 heat-response regulon of Arabidopsis thaliana. (unr.edu)
  • Here we report on the identification of a new heat-response regulon in plants controlled by the multiprotein bridging factor 1c (MBF1c) protein of Arabidopsis thaliana. (unr.edu)
  • A comparative genomics approach was used to reconstruct the L-Rha catabolic pathways and transcriptional regulons in the phyla Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Proteobacteria, and Thermotogae. (frontiersin.org)
  • Telefono Casa Del Libro Galactose Regulon of Yeast: From Genetics to Systems Biology Pedidos Online. (startupkenya.org)
  • Leer Libros Galactose Regulon of Yeast: From Genetics to Systems Biology On Line En EPUB. (startupkenya.org)
  • At any rate, I'm expecting that RNA regulons will be increasingly important in understanding the translational regulation that must take place in dendrites to produce persistent memories. (gnxp.com)
  • Regulation of MalT and its regulon. (hindawi.com)
  • Regulation of the SOS response regulon in E. coli. (coursehero.com)
  • ToxT is the most downstream regulator of the ToxR regulon in that it can activate ctx and tcp promoters independently once ToxT expression has occurred ( 9 , 10 ). (pnas.org)
  • The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules. (sigmaaldrich.com)
  • Both phosphorylated and unphosphorylated forms of the cognate response regulator RegA are capable of activating or repressing a variety of genes in the regulon. (asm.org)
  • The two component sensor/regulator dosRS is a major mediator in the transcriptional response of M. tuberculosis to hypoxia and controls a regulon of approximately 50 genes that are induced under this condition. (cam.ac.uk)
  • A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae. (nih.gov)
  • Activation of the sigma(M) regulon increases expression of proteins functioning in transcriptional control, cell wall synthesis and shape determination, cell division, DNA damage monitoring, recombinational repair and detoxification. (nih.gov)
  • In addition to the Fur regulon, iron starvation induces members of the PerR regulon and leads to reduced expression of cytochromes. (nih.gov)
  • The PhoP regulon is a well studied two component (PhoP/Q) coordinately regulated network of genes whose expression is required for intracellular survival of S. enterica. (harvard.edu)
  • In accordance with the well-known phenomenon that salt stress promotes early leaf senescence in many plant species, we previously observed salt stress-enhanced expression of many SAGs of the ANAC092 regulon. (pubmedcentralcanada.ca)
  • We investigated the role of GlnR in the nitrogen limitation response and determined the entire GlnR regulon , by combining expression profiling of M. smegmatis wild type and glnR deletion mutant, with GlnR-specific chromatin immunoprecipitation and high throughput sequencing. (biomedcentral.com)
  • In the present work we have used proteomic and microarray expression profiling of E. faecalis JH2-2 and an ers-deleted mutant (Δ ers mutant) strains to define the Ers regulon. (archives-ouvertes.fr)
  • The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. (umass.edu)
  • Functional and evolutionary analyses of these mutations revealed parallel phenotypic decreases in the maltose regulon expression and a high level of polymorphism at this locus in the evolved populations. (genetics.org)
  • The aim of this study was to determine whether the induction of the entire DosR regulon is triggered as a synchronous event or if induction can unfold as a cascade of events as the differential expression of subsets of genes is stimulated by different oxygen availabilities. (cam.ac.uk)
  • The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses. (nih.gov)
  • The main objective of this study was to analyze control of these genes in different growth mediums and determine if Crc is a transcriptional protein or a post-transcriptional controller of the regulon. (uncg.edu)
  • During the divergence of these organisms from a common "SigB-less" ancestor, the placement of SigB promoters at varied locations in the B. cereus sensu lato genomes predict alternative structures for the SigB regulon in different organisms. (biomedcentral.com)
  • Further, we identified nonsynonymous mutations in major DosR regulon genes of 44 L6 genomes of TB patients from The Gambia and Ghana. (itg.be)
  • To characterize the HrpL regulon, we are currently following two strategies (i) the bioinformatics identification of HrpL-dependent promoters in the genomes of several P. savastanoi pathovars and, (ii) a comparative RNAseq analysis of a wild-type Psv strain and its ∆hrpL mutant. (uma.es)
  • By analysing multiple microarray-based transcriptional datasets, we have extended that study and now suggest that the ribosomal and rRNA biosynthesis regulon contains over 200 genes. (nih.gov)
  • These results demonstrated that a subgenomic oligonucleotide microarray is a powerful tool for defining the SalA regulon and its relationship to other genes important to plant pathogenesis. (apsnet.org)
  • DNA microarray and proteomic analyses of the RpoS regulon in Geobacter sulfurreducens. (umass.edu)
  • We now demonstrate that the evolution of the regulons governed by the regulatory protein PhoP in the related human pathogens Salmonella enterica and Yersinia pestis has entailed functional changes in the PhoP protein as well as in the architecture of PhoP-dependent promoters. (pnas.org)
  • Sixty-six genes were found to belong to the PerR regulon. (omicsdi.org)
  • To identify the PerR regulon, the wild-type strain C. jejuni NCTC 11168 and the perR mutant were grown in 500 ml flasks containing 250 ml of MEMa medium. (omicsdi.org)
  • The DosR regulon was the most significantly decreased category in L6 relative to L4. (itg.be)
  • In the host, the DosR regulon is critical for M. tuberculosis in adaptation to oxygen limitation. (itg.be)
  • To define the ToxR regulon more fully, mutants defective in these regulatory genes were transcriptionally profiled by using V. cholerae genomic microarrays. (pnas.org)
  • The Reg regulon from Rhodobacter capsulatus and Rhodobacter sphaeroides encodes proteins involved in numerous energy-generating and energy-utilizing processes such as photosynthesis, carbon fixation, nitrogen fixation, hydrogen utilization, aerobic and anaerobic respiration, denitrification, electron transport, and aerotaxis. (asm.org)
  • Previously, the gene cg0156 was shown to belong to the regulon of McbR, a global transcriptional repressor of sulphur metabolism in C. glutamicum. (sigmaaldrich.com)
  • Multiple epidemiologic studies suggest that strains expressing AggR are more likely to cause diarrheal disease than those without it, leading us to propose the term "typical EAEC" to describe strains harboring the as-yet-incompletely characterized AggR regulon ( 8 , 12 , 13 ). (asm.org)
  • M. tuberculosis RV3134 recombinant protein of dormancy regulon. (lsbio.com)
  • A wealth of genetic information and some biochemical analysis have made the GAL regulon of the yeast Saccharomyces cerevisiae a classic model system for studying transcriptional activation in eukaryotes. (cshl.edu)
  • Yeast Feast on Xylose and Grow Quickly If Regulon Is Table. (genengnews.com)
  • We have used DNA microarrays to define the Fur regulon and the iron starvation stimulon. (nih.gov)
  • Here, we identified an ovarian lineage-specific PAX8 regulon using modified cancer outlier profile analysis, in which PAX8-FGF18 axis was responsible for promoting cell migration in an autocrine fashion. (elifesciences.org)
  • Directional RNAseq analyses was undertaken in Klebsiella pneumoniae Ecl8 and isogenic mutants Ecl8delta ramA and Ecl8delta ramR to determine the RamA regulon. (omicsdi.org)
  • The activity of the enzymes in the hex regulon are required for catabolism of glucose and these activities are coordinately regulated. (uncg.edu)
  • The heat shock response in E. coli is regulated by the sigma factor σ32 (RpoH), whose regulon has been characterized as containing at least 89 open reading frames. (wikipedia.org)
  • The availability of the complete genome sequence and pan- Neisseria microarrays provide an opportunity to test these assumptions directly by comparing the extent of the N. gonorrhoeae FNR regulon with that of the recently-published E. coli FNR regulon [ 8 ]. (biomedcentral.com)
  • Li, Yu ( https://orcid.org/0000-0003-4359-4411 ), "Delineating the Upc2A Regulon in Candida glabrata" (2021). (uthsc.edu)
  • Thus, chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) was performed to confirm the putative regulon of MAP3773c (Fur-like protein) in MAP. (unl.edu)
  • Identification of the REST regulon reveals extensive transposable element-mediated binding site duplication. (ox.ac.uk)
  • This regulon is distinct from the set of ribosomal protein genes, and the promoters of the expanded RRB gene set are highly enriched for the PAC and RRPE motifs. (nih.gov)
  • OmpR , OmpR regulon and binding site collection in Yersinia pestis biovar Microtus str. (collectf.org)
  • Here, we show that in addition to O(2) and NO, CO induces the complete Mtb dormancy (Dos) regulon. (uea.ac.uk)
  • Also, most interesting for my neuro-tastes is the recent report from the Moore lab showing that the immediate-early gene implicated in neuronal homeostasis, Arc, may be part of a regulon defined by introns in the 3'UTR. (gnxp.com)
  • Thus, subcellular hnRNP-interacting RNA regulons support neuronal growth and regeneration. (mcponline.org)
  • We aimed to delineate the Upc2A regulon to determine its target genes involved in FLU resistance. (uthsc.edu)
  • The network of regulons reveals a higher-level organization, with dense local neighborhoods articulated for photosynthetic function, genetic information processing, and stress response. (beds.ac.uk)