Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Recombinant Fusion Proteins
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.
Sequence Homology, Amino Acid
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Electrophoresis, Polyacrylamide Gel
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The class Insecta, in the phylum ARTHROPODA, whose members are characterized by division into three parts: head, thorax, and abdomen. They are the dominant group of animals on earth; several hundred thousand different kinds having been described. Three orders, HEMIPTERA; DIPTERA; and SIPHONAPTERA; are of medical interest in that they cause disease in humans and animals. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p1)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Sequence Analysis, DNA
Open Reading Frames
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The internal fragments of precursor proteins (INternal proTEINS) that are autocatalytically removed by PROTEIN SPLICING. The flanking fragments (EXTEINS) are ligated forming mature proteins. The nucleic acid sequences coding for inteins are considered to be MOBILE GENETIC ELEMENTS. Inteins are composed of self-splicing domains and an endonuclease domain which plays a role in the spread of the intein's genomic sequence. Mini-inteins are composed of the self-splicing domains only.
Green Fluorescent Proteins
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Plants, Genetically Modified
Protein Structure, Secondary
A generic term for any circumscribed mass of foreign (e.g., lead or viruses) or metabolically inactive materials (e.g., ceroid or MALLORY BODIES), within the cytoplasm or nucleus of a cell. Inclusion bodies are in cells infected with certain filtrable viruses, observed especially in nerve, epithelial, or endothelial cells. (Stedman, 25th ed)
Viral Envelope Proteins
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
Chromatography, High Pressure Liquid
Bacterial Outer Membrane Proteins
Protein Sorting Signals
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Gene Transfer Techniques
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Recombinant DNA vectors encoding antigens administered for the prevention or treatment of disease. The host cells take up the DNA, express the antigen, and present it to the immune system in a manner similar to that which would occur during natural infection. This induces humoral and cellular immune responses against the encoded antigens. The vector is called naked DNA because there is no need for complex formulations or delivery agents; the plasmid is injected in saline or other buffers.
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Sensitivity and Specificity
Dose-Response Relationship, Drug
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Promoter Regions, Genetic
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Tumor Cells, Cultured
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Organisms, Genetically Modified
Amino Acid Substitution
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
A genus of silkworm MOTHS in the family Bombycidae of the order LEPIDOPTERA. The family contains a single species, Bombyx mori from the Greek for silkworm + mulberry tree (on which it feeds). A native of Asia, it is sometimes reared in this country. It has long been raised for its SILK and after centuries of domestication it probably does not exist in nature. It is used extensively in experimental GENETICS. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p519)
Reverse Transcriptase Polymerase Chain Reaction
Fluorescent Antibody Technique, Indirect
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Surface Plasmon Resonance
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Gene Expression Regulation, Bacterial
Merozoite Surface Protein 1
Amino Acid Motifs
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
Gene Expression Regulation
Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation. (1/70987)The product of the oskar gene directs posterior patterning in the Drosophila oocyte, where it must be deployed specifically at the posterior pole. Proper expression relies on the coordinated localization and translational control of the oskar mRNA. Translational repression prior to localization of the transcript is mediated, in part, by the Bruno protein, which binds to discrete sites in the 3' untranslated region of the oskar mRNA. To begin to understand how Bruno acts in translational repression, we performed a yeast two-hybrid screen to identify Bruno-interacting proteins. One interactor, described here, is the product of the apontic gene. Coimmunoprecipitation experiments lend biochemical support to the idea that Bruno and Apontic proteins physically interact in Drosophila. Genetic experiments using mutants defective in apontic and bruno reveal a functional interaction between these genes. Given this interaction, Apontic is likely to act together with Bruno in translational repression of oskar mRNA. Interestingly, Apontic, like Bruno, is an RNA-binding protein and specifically binds certain regions of the oskar mRNA 3' untranslated region. (+info)
Mechanisms of GDF-5 action during skeletal development. (2/70987)Mutations in GDF-5, a member of the TGF-beta superfamily, result in the autosomal recessive syndromes brachypod (bp) in mice and Hunter-Thompson and Grebe-type chondrodysplasias in humans. These syndromes are all characterised by the shortening of the appendicular skeleton and loss or abnormal development of some joints. To investigate how GDF-5 controls skeletogenesis, we overexpressed GDF-5 during chick limb development using the retrovirus, RCASBP. This resulted in up to a 37.5% increase in length of the skeletal elements, which was predominantly due to an increase in the number of chondrocytes. By injecting virus at different stages of development, we show that GDF-5 can increase both the size of the early cartilage condensation and the later developing skeletal element. Using in vitro micromass cultures as a model system to study the early steps of chondrogenesis, we show that GDF-5 increases chondrogenesis in a dose-dependent manner. We did not detect changes in proliferation. However, cell suspension cultures showed that GDF-5 might act at these stages by increasing cell adhesion, a critical determinant of early chondrogenesis. In contrast, pulse labelling experiments of GDF-5-infected limbs showed that at later stages of skeletal development GDF-5 can increase proliferation of chondrocytes. Thus, here we show two mechanisms of how GDF-5 may control different stages of skeletogenesis. Finally, our data show that levels of GDF-5 expression/activity are important in controlling the size of skeletal elements and provides a possible explanation for the variation in the severity of skeletal defects resulting from mutations in GDF-5. (+info)
Sonic hedgehog signaling by the patched-smoothened receptor complex. (3/70987)BACKGROUND: The Hedgehog (Hh) family of secreted proteins is involved in a number of developmental processes as well as in cancer. Genetic and biochemical data suggest that the Sonic hedgehog (Shh) receptor is composed of at least two proteins: the tumor suppressor protein Patched (Ptc) and the seven-transmembrane protein Smoothened (Smo). RESULTS: Using a biochemical assay for activation of the transcription factor Gli, a downstream component of the Hh pathway, we show here that Smo functions as the signaling component of the Shh receptor, and that this activity can be blocked by Ptc. The inhibition of Smo by Ptc can be relieved by the addition of Shh. Furthermore, oncogenic forms of Smo are insensitive to Ptc repression in this assay. Mapping of the Smo domains required for binding to Ptc and for signaling revealed that the Smo-Ptc interaction involves mainly the amino terminus of Smo, and that the third intracellular loop and the seventh transmembrane domain are required for signaling. CONCLUSIONS: These data demonstrate that Smo is the signaling component of a multicomponent Hh receptor complex and that Ptc is a ligand-regulated inhibitor of Smo. Different domains of Smo are involved in Ptc binding and activation of a Gli reporter construct. The latter requires the third intracellular loop and the seventh transmembrane domain of Smo, regions often involved in coupling to G proteins. No changes in the levels of cyclic AMP or calcium associated with such pathways could be detected following receptor activation, however. (+info)
Relaxin is a potent renal vasodilator in conscious rats. (4/70987)The kidneys and other nonreproductive organs vasodilate during early gestation; however, the "pregnancy hormones" responsible for the profound vasodilation of the renal circulation during pregnancy are unknown. We hypothesized that the ovarian hormone relaxin (RLX) contributes. Therefore, we tested whether the administration of RLX elicits renal vasodilation and hyperfiltration in conscious adult, intact female rats. After several days of treatment with either purified porcine RLX or recombinant human RLX 2 (rhRLX), effective renal plasma flow (ERPF) and glomerular filtration rate (GFR) increased by 20%-40%. Comparable renal vasodilation and hyperfiltration was also observed in ovariectomized rats, suggesting that estrogen and progesterone are unnecessary for the renal response to rhRLX. The nitric oxide synthase inhibitor Nomega-nitro-L-arginine methyl ester completely abrogated the increase in ERPF and GFR elicited by chronic administration of purified porcine RLX. In contrast, the renal vasoconstrictory response to angiotensin II was attenuated by the RLX treatment. Short-term infusion of purified porcine RLX to conscious rats over several hours failed to increase ERPF and GFR. Plasma osmolality was consistently reduced by the chronic administration of both RLX preparations. In conclusion, the renal and osmoregulatory effects of chronic RLX administration to conscious rats resemble the physiological changes of pregnancy in several respects: (a) marked increases in ERPF and GFR with a mediatory role for nitric oxide; (b) attenuation of the renal circulatory response to angiotensin II; and (c) reduction in plasma osmolality. (+info)
Caspase-mediated cleavage of p21Waf1/Cip1 converts cancer cells from growth arrest to undergoing apoptosis. (5/70987)The cyclin-dependent kinase inhibitor p21waf1/Cip1 is a downstream effector of the p53-dependent cell growth arrest. We report herein that p21 was cleaved by caspase-3/CPP32 at the site of DHVD112L during the DNA damage-induced apoptosis of cancer cells. The cleaved p21 fragment could no more arrest the cells in G1 phase nor suppress the cells undergoing apoptosis because it failed to bind to the proliferating cell nuclear antigen (PCNA) and lost its capability to localize in the nucleus. Thus, caspase-3-mediated cleavage and inactivation of p21 protein may convert cancer cells from growth arrest to undergoing apoptosis, leading to the acceleration of chemotherapy-induced apoptotic process in cancer cells. (+info)
Concomitant activation of pathways downstream of Grb2 and PI 3-kinase is required for MET-mediated metastasis. (6/70987)The Met tyrosine kinase - the HGF receptor - induces cell transformation and metastasis when constitutively activated. Met signaling is mediated by phosphorylation of two carboxy-terminal tyrosines which act as docking sites for a number of SH2-containing molecules. These include Grb2 and p85 which couple the receptor, respectively, with Ras and PI 3-kinase. We previously showed that a Met mutant designed to obtain preferential coupling with Grb2 (Met2xGrb2) is permissive for motility, increases transformation, but - surprisingly - is impaired in causing invasion and metastasis. In this work we used Met mutants optimized for binding either p85 alone (Met2xPI3K) or p85 and Grb2 (MetPI3K/Grb2) to evaluate the relative importance of Ras and PI 3-kinase as downstream effectors of Met. Met2xPI3K was competent in eliciting motility, but not transformation, invasion, or metastasis. Conversely, MetP13K/Grb2 induced motility, transformation, invasion and metastasis as efficiently as wild type Met. Furthermore, the expression of constitutively active PI 3-kinase in cells transformed by the Met2xGrb2 mutant, fully rescued their ability to invade and metastasize. These data point to a central role for PI 3-kinase in Met-mediated invasiveness, and indicate that simultaneous activation of Ras and PI 3-kinase is required to unleash the Met metastatic potential. (+info)
C-myc overexpression and p53 loss cooperate to promote genomic instability. (7/70987)p53 monitors genomic integrity at the G1 and G2/M cell cycle checkpoints. Cells lacking p53 may show gene amplification as well as the polyploidy or aneuploidy typical of many tumors. The pathways through which this develops, however, are not well defined. We demonstrate here that the combination of p53 inactivation and c-myc overexpression in diploid cells markedly accelerates the spontaneous development of tetraploidy. This is not seen with either N-myc or L-myc. Tetraploidy is accompanied by significantly higher levels of cyclin B and its associated cdc2 kinase activity. Mitotic spindle poisons accelerate the appearance of tetraploidy in cells either lacking functional p53 or overexpressing c-myc whereas the combination is additive. Restoration of p53 function in cells overexpressing c-myc causing rapid apoptosis, indicating that cells yet to become tetraploid have nonetheless suffered irreversible genomic and/or mitotic spindle damage. In the face of normal p53 function, such damage would either be repaired or trigger apoptotis. We propose that loss of p53 and overexpression of c-myc permits the emergence and survival of cells with increasingly severe damage and the eventual development of tetraploidy. (+info)
TIF1gamma, a novel member of the transcriptional intermediary factor 1 family. (8/70987)We report the cloning and characterization of a novel member of the Transcriptional Intermediary Factor 1 (TIF1) gene family, human TIF1gamma. Similar to TIF1alpha and TIF1beta, the structure of TIF1beta is characterized by multiple domains: RING finger, B boxes, Coiled coil, PHD/TTC, and bromodomain. Although structurally related to TIF1alpha and TIF1beta, TIF1gamma presents several functional differences. In contrast to TIF1alpha, but like TIF1beta, TIF1 does not interact with nuclear receptors in yeast two-hybrid or GST pull-down assays and does not interfere with retinoic acid response in transfected mammalian cells. Whereas TIF1alpha and TIF1beta were previously found to interact with the KRAB silencing domain of KOX1 and with the HP1alpha, MODI (HP1beta) and MOD2 (HP1gamma) heterochromatinic proteins, suggesting that they may participate in a complex involved in heterochromatin-induced gene repression, TIF1gamma does not interact with either the KRAB domain of KOX1 or the HP1 proteins. Nevertheless, TIF1gamma, like TIF1alpha and TIF1beta, exhibits a strong silencing activity when tethered to a promoter. Since deletion of a novel motif unique to the three TIF1 proteins, called TIF1 signature sequence (TSS), abrogates transcriptional repression by TIF1gamma, this motif likely participates in TIF1 dependent repression. (+info)
Phase I trial of recombinant human gamma-interferon and recombinant human tumor necrosis factor in patients with advanced...
Phase I trial of recombinant human gamma-interferon and recombinant human tumor necrosis factor in patients with advanced gastrointestinal cancer.
Activation of recombinant human protein C<...
TY - JOUR. T1 - Activation of recombinant human protein C. AU - Lee, Timothy K.. AU - Bangalore, Neelesh. AU - Velander, William. AU - Drohan, William N.. AU - Lubon, Henryk. PY - 1996/5/1. Y1 - 1996/5/1. N2 - We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have analyzed the interaction of the zymogen with Protac, thrombin/thrombomodulin and thrombin alone. The amidolytic and anticoagulant activities of rAPC after Protac activation were ~80% those of its human plasma counterpart. Upon the excision of the activation peptide by thrombin/thrombomodulin complex, both the natural and recombinant activation products had similar enzymatic and biological activities. This. observation can be attributed to the difference in the mechanism of action between the two activators and structural differences between HPC and rHPC.. AB - We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have ...
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Recombinant Protein Market to Grow at a CAGR of 11.2% during the Forecast Period | Quince Market Insights |Market Research &...
The Global Recombinant Protein Market is anticipated to grow at a CAGR of 11.2% during the forecast period. Recombinant proteins are used in designing new treatments for serious chronic disorders such as cancer and other rare diseases. A special technique known as recombinant DNA (rDNA) technology is used for recombinant protein production. Recombinant proteins are categorized into hormones, growth factor, cytokines, plasma protein factor, recombinant metabolic enzymes, and others, depending on the type of protein.. Recombinant technology is the mechanism involved in the production of industrial recombinant proteins. Industrial recombinant proteins and their derivatives have various applications in industries such as food, pesticides, pharmaceuticals, veterinary, agriculture, and detergents. Due to this, the industrial recombinant protein market is gaining a lot of attention worldwide. As a result, the producers of industrial recombinant protein have found great market opportunities.. Countries ...
Recombinant Human Protein SCO1 Homolog Mitochondrial SCO1/SCOD1 (N-GST) | Bon Opus Biosciences
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Aldehyde Dehydrogenase 1 family, member A3 (ALDH1A3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein stucture analysis and in cell biology research applications.
AMPK (A1/B2/G2) Recombinant Human Protein
This AMPK (A1/B2/G2) recombinant human protein (full length) was expressed in insect cells. AMPK (A1/B2/G2) serine/threonine kinase or AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a c
Antiviral effects of recombinant human tumor necrosis factor-alpha in combination with natural interferon-beta in mice infected...
TY - JOUR. T1 - Antiviral effects of recombinant human tumor necrosis factor-alpha in combination with natural interferon-beta in mice infected with herpes simplex virus type 1. AU - Schmitt, David A.. AU - Sasaki, Hidetaka. AU - Pollard, Richard B.. AU - Suzuki, Fujio. PY - 1992/10/1. Y1 - 1992/10/1. N2 - The protective effects of combination therapy utilizing recombinant human TNF-alpha (rTNF-α) and natural murine interferon-beta (IFN-β) in mice infected with herpes simplex virus type 1 (HSV-1) was investigated. Mice treated with rTNF-α alone at all of the doses tested (a single i.v. administration, 2.3-2,300 μg/kg; multiple i.p. administrations 0.4-250 μg/kg) as well as mice that received IFN-β alone at doses of 16 × 104 U/kg or less resulted in a 0% survival rate. Combination therapy consisting of a single administration of rTNF- α (230 and 23 μg/kg) and multiple administrations of IFN-β (4 × 104 U/kg) resulted in a 40% and 60% survival rate. Multiple treatments of infected mice ...
Antiviral effects of recombinant human tumor necrosis factor-alpha in combination with natural interferon-beta in mice infected...
TY - JOUR. T1 - Antiviral effects of recombinant human tumor necrosis factor-alpha in combination with natural interferon-beta in mice infected with herpes simplex virus type 1. AU - Schmitt, David A.. AU - Sasaki, Hidetaka. AU - Pollard, Richard B. AU - Suzuki, Fujio. PY - 1992/10/1. Y1 - 1992/10/1. N2 - The protective effects of combination therapy utilizing recombinant human TNF-alpha (rTNF-α) and natural murine interferon-beta (IFN-β) in mice infected with herpes simplex virus type 1 (HSV-1) was investigated. Mice treated with rTNF-α alone at all of the doses tested (a single i.v. administration, 2.3-2,300 μg/kg; multiple i.p. administrations 0.4-250 μg/kg) as well as mice that received IFN-β alone at doses of 16 × 104 U/kg or less resulted in a 0% survival rate. Combination therapy consisting of a single administration of rTNF- α (230 and 23 μg/kg) and multiple administrations of IFN-β (4 × 104 U/kg) resulted in a 40% and 60% survival rate. Multiple treatments of infected mice ...
The Effect of Human Recombinant Tumor Necrosis Factor Receptor-2 on Reducing Inflammatory of Collagen -Induced Arthritis in...
Background: The tumor necrosis factor alpha (TNFα) is a cytokine that produced principally by monocyte/macrophages and T lymphocytes, respectively. TNFα is recognized as the primary mediator of immunity in inflammation reaction. One important application of Tumor Necrosis Factor Receptor 2 (TNFR2) is for the treatment of autoimmune diseases like rheumatoid arthritis (RA). Objectives: The aim of this study is to examine the therapeutic trace of the recombinant humanTNFR2 on collagen-induced arthritis (CIA) in mice. Materials and Methods: CIA was created in 20 mice by immunization with bovine type II collagen (CII). After the mice were boosted on day 21, they were injected with the recombinant protein in test group (1 mg.kg-1) and assessed edema in paws and knee joints after two weeks. The quantities of inflammatory cytokines such as TNF-α, interleukin-1 beta (IL-β1), interleukin-6 (IL-6), and interleukin-10(IL-10) in serum were evaluated through enzyme-linked immunosorbent assay (ELISA) kit. In
Nutritional parameters observed during 28-day infusion of recombinant human tumor necrosis factor-α<...
TY - JOUR. T1 - Nutritional parameters observed during 28-day infusion of recombinant human tumor necrosis factor-α. AU - Hardin, T. C.. AU - Koeller, J. M.. AU - Kuhn, J. G.. AU - Roodman, G. D.. AU - Von Hoff, D. D.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - In conjunction with a Phase I investigation of the antineoplastic activity of recombinant human tumor necrosis factor-α (TNF-α), administered as a 28- day continuous infusion, selected nutritional parameters were evaluated to identify any effect that might be attributed to the TNF infusion. Seven clinically stable men with a variety of tumor types were studied. None had clinical or laboratory evidence of significant malnutrition before entry into the study. Five patients received 10 μg of recombinant human TNF-α per square meter per day and two patients received 25 μg/m2 per day. Indirect calorimetry assessment of resting energy expenditure, body weight, serum TNF concentration, and laboratory analysis of common nutritional markers ...
Recombinant Human Epidermal Growth Factor EGF-556H - Creative BioMart
A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. Recombinant Human Epidermal Growth Factor produced in E.Coli is a single, non-glycosylated, polype
EORTC (30885) randomised phase III study with recombinant interferon alpha and recombinant interferon alpha and gamma in...
In the treatment of renal cell carcinoma both complete (CRs) and partial remissions (PRs) have been obtained using recombinant (r) interferon alpha (IFN-alpha), with response rates ranging from 0 to 31% (mean 16%). rIFN-gamma is a potent immunostimulating agent, but the clinical experience of its use is limited and results are conflicting. In a phase II study with the combination of rIFN-alpha(2c) (Boehringer Ingelheim) and rIFN-gamma (Genentech, supplied by Boehringer Ingelheim) in 31 eligible patients, a response rate of 25% was recorded. Based on this observation a randomised phase III study was initiated to investigate the possible advantage of the addition rIFN-gamma to rIFN-alpha(2c) treatment. Treatment consisted of rIFN-alpha(2c) 30 pg m(-2) = 10 x 10(6) IU m(-2) s.c. twice weekly in arm A and the same dose of rIFN-alpha combined with rIFN-gamma 100 mu g m(-2) = 2 x 10(6) IU m(-2) in arm B. Eligibility criteria included documented progression of disease; patients with bone lesions only ...
Construction and properties of fusion proteins between human interferon-gamma and human tumour necrosis factors alpha and beta....
A number of recombinant plasmids coding for fusion proteins between human interferon-gamma (IFN-gamma) and human tumour necrosis factor alpha (TNF alpha) or beta (TNF beta) were constructed by using site-directed mutagenesis and ligation of the respective genes. In these proteins the whole IFN-gamma sequence of the molecule is linked at the N terminus via a short polypeptide linker to the TNF alpha sequence lacking two N-terminal amino acid residues or to the whole TNF beta sequence. A series of mutants with deletions in the interferon part of the fusion proteins were also produced. All the fusion genes obtained were efficiently expressed in Escherichia coli under the control of early promoters of bacteriophage T7. The recombinant fusion proteins were found to be unstable inside bacterial cells. Bacterial cell lysates expressing these fusion genes or their deletion mutants showed both biological activities in vitro: the antiviral activity of IFN-gamma and the cytotoxic activity of TNF.
Studies on the anti-tumor efficacy of systemically administered recombinant tumor necrosis factor against several murine tumors...
The anti-tumor activity of recombinant human tumor necrosis factor (rHTNF) was examined against four newly induced murine sarcomas (MCA-101, -102, -105, and -106) and a murine adenocarcinoma (MCA-38) transplanted s.c. into C57BL/6 mice. The serum half-life after a single i.v. injection of rHTNF was determined to be 30 +/- 2 min. Tumor-bearing mice were more susceptible to the toxic side effects of rHTNF than were normal mice. Forty-eight percent (41/86) of tumor bearing animals that received 10 micrograms rHTNF died within 48 hr after treatment compared with no deaths in 28 normal animals receiving this dose. Treatment of mice bearing either the MCA-101, -102, -105, or -106 sarcoma or the MCA-38 adenocarcinoma with rHTNF resulted in a marked necrosis of the central portion of each tumor within 24 hr. Animals bearing the weakly immunogenic tumors MCA-105, -106, and -38 experienced a reduction in average tumor area of 47% +/- 5, 46% +/- 6, and 37% +/- 11, respectively, by 3 to 4 days after ...
Extended cell-free protein expression system for amino acid labeling and structural biology at Miami Universitys Center for...
Project Title: Extended cell-free protein expression system for amino acid labeling and structural biology studies at Miami Universitys Center for Bioinformatics and Functional Genomics (CBFG).. Project Lead: Carole Dabney-Smith. Email: [email protected] Phone: (513) 529-8091. Affiliation: CAS. Other Team Members: Andor Kiss, Gary A. Lorigan. Project Details: This project is a request for a temperature regulated, heating and cooling capable, mixing, dry incubator with accessories capable of handling tube with different volumes and a starter expression kit. This will enable undergraduate and graduate student users to produce microgram to milligram quantities of in vitro expressed protein in a semi-high throughput fashion to aid in the investigation of protein structure/function relationships of any protein. One consistent barrier that impedes the progress of, discovery of structure/function relationships has been the ability to reliably and quickly generate protein, e.g., with single amino ...
Recombinant human thrombopoietin in combination with granulocyte colony- stimulating factor enhances mobilization of peripheral...
TY - JOUR. T1 - Recombinant human thrombopoietin in combination with granulocyte colony- stimulating factor enhances mobilization of peripheral blood progenitor cells, increases peripheral blood platelet concentration, and accelerates hematopoietic recovery following high-dose chemotherapy. AU - Somlo, George. AU - Sniecinski, Irena. AU - Ter Veer, Anna. AU - Longmate, Jeffrey. AU - Knutson, Gaylord. AU - Vuk-Pavlovic, Stanimir. AU - Bhatia, Ravi. AU - Chow, Warren. AU - Leong, Lucille. AU - Morgan, Robert. AU - Margolin, Kim. AU - Raschko, James. AU - Shibata, Stephen. AU - Tetef, Merry. AU - Yen, Yun. AU - Forman, Stephen. AU - Jones, Dennie. AU - Ashby, Mark. AU - Fyfe, Gwen. AU - Hellmann, Susan. AU - Doroshow, James H.. PY - 1999/5/1. Y1 - 1999/5/1. N2 - Lineage-specific growth factors mobilize peripheral blood progenitor cells (PBPC) and accelerate hematopoietic recovery after high-dose chemotherapy. Recombinant human thrombopoietin (rhTPO) may further increase the progenitor-cell content ...
This study has examined the effect of the cytokine interleukin 1 (IL-1) on metastasis formation by the human melanoma A375M in nude mice. We have found that human recombinant IL-1β (a single injection ,0.01 µg per mouse i.v. given before tumor cells) induced an augmentation of experimental lung metastases from the A375M tumor cells in nude mice. This effect was rapidly induced and reversible within 24 h after IL-1 injection. A similar effect was induced by human recombinant IL-1α and human recombinant tumor necrosis factor, but not by human recombinant interleukin 6. 5-[125I]Iodo-2′-deoxyuridine-radiolabeled A375M tumor cells injected i.v. remained at a higher level in the lungs of nude mice receiving IL-1 than in control mice. In addition, IL-1 injected 1 h, but not 24 h, after tumor cells enhanced lung colonization as well, thus suggesting an effect of IL-1 on the vascular transit of tumor cells. These findings may explain the observation of enhanced secondary localization of tumor cells ...
Cell-type-specific regulation of the human tumor necrosis factor alpha gene in B cells and T cells by NFATp and ATF-2/JUN. |...
The human tumor necrosis factor alpha (TNF-alpha) gene is one of the earliest genes transcribed after the stimulation of a B cell through its antigen receptor or via the CD-40 pathway. In both cases, induction of TNF-alpha gene transcription can be blocked by the immunosuppressants cyclosporin A and FK506, which suggested a role for the NFAT family of proteins in the regulation of the gene in B cells. Furthermore, in T cells, two molecules of NFATp bind to the TNF-alpha promoter element kappa 3 in association with ATF-2 and Jun proteins bound to an immediately adjacent cyclic AMP response element (CRE) site. Here, using the murine B-cell lymphoma cell line A20, we show that the TNF-alpha gene is regulated in a cell-type-specific manner. In A20 B cells, the TNF-alpha gene is not regulated by NFATp bound to the kappa 3 element. Instead, ATF-2 and Jun proteins bind to the composite kappa 3/CRE site and NFATp binds to a newly identified second NFAT site centered at -76 nucleotides relative to the ...
A controlled study of recombinant human granulocyte colony-stimulating factor in elderly patients after treatment for acute...
The mortality rate at eight weeks was similar in the lenograstim and placebo groups (23 and 27 percent, respectively; P = 0.60), as was the incidence of severe infections. The median duration of neutropenia (absolute neutrophil count , or = 1000 per cubic millimeter) was shorter in the lenograstim group (21 days, as compared with 27 days in the placebo group; P , 0.001). Eight percent of the patients in both groups had regrowth of AML cells. The rate of complete remission was significantly higher in the lenograstim group (70 percent, as compared with 47 percent in the placebo group; P = 0.002). Overall survival, however, was similar in the two groups (P = 0.76). Conclusions: ...
The Effect of Recombinant Human Erythropoietin on Neurovasculature Repair after Focal Ischemic Stroke in Neonatal Rats |...
Animal Model. Neonatal Wistar rats were randomly divided into three groups: 1) no-stroke control, 2) saline control, and 3) rhEPO treatment. The surgical procedure of whisker-barrel cortex ischemia in neonatal rats followed similar methods as described previously (Wei et al., 2006). In brief, postnatal day 7 (P7) pups were anesthetized by hypothermia. Hypothermia anesthesia was chosen because many of the drugs used to anesthetize adult animals provided inadequate anesthesia for neonates or were associated with problems such as excessively high mortality (Danneman and Mandrell, 1997). In this regard, hypothermia (immersion in ice) has been judged as a humane, safe, and effective anesthesia method for survival surgeries of neonatal rats (Danneman and Mandrell, 1997). The hypothermia procedure was kept the same for all pups in different experimental groups. Pups were placed in a noninvasive head-holder to allow for a 2.5- to 3.0-mm-diameter craniectomy through the right parietal skull. The ...
Ipilimumab With or Without High-Dose Recombinant Interferon Alfa-2b in Treating Patients With Stage III-IV Melanoma That Cannot...
Patients must not have autoimmune disorders or conditions of immunosuppression that require current ongoing treatment with systemic corticosteroids (or other systemic immunosuppressants), including oral steroids (e.g., prednisone, dexamethasone) or continuous use of topical steroid creams or ointments or ophthalmologic steroids; a history of occasional (but not continuous) use of steroid inhalers is allowed; replacement doses of steroids for patients with adrenal insufficiency are allowed; patients who discontinue use of these classes of medication for at least 2 weeks prior to randomization are eligible if, in the judgment of the treating physician investigator, the patient is not likely to require resumption of treatment with these classes of drugs during the study; exclusion from this study also includes patients with a history of symptomatic autoimmune disease (e.g., rheumatoid arthritis, systemic progressive sclerosis [scleroderma], systemic lupus erythematosus, Sjogrens syndrome, ...
CD3E Recombinant Monoclonal Antibody [BL-298-5D12] | Bethyl Laboratories, Inc.
Unconjugated Whole IgG Rabbit anti-CD3E Recombinant Monoclonal Antibody [BL-298-5D12] suitable for WB, IP, IHC, ICC, IHC-IF, F, mIF applications. Visit Bethyl.com for all your antibody needs.
Difference Between Parental Type and Recombinant Type Chromosomes l Parental Type vs Recombinant Type Chromosomes
What is the difference between Parental Type and Recombinant Type Chromosomes? Crossover not occurs in parental type chromosomes; in recombinant type
Recombinant Human Interleukin-5 克拉玛尔
克拉玛尔专业提供Recombinant Human Interleukin-5高品质试剂,可查询Recombinant Human Interleukin-5价格,提供Recombinant Human Interleukin-5技术资料,Recombinant Human Interleukin-5定制合成和大包装,是专业生产各种高端试剂的制造商和供应商.联系电话4001650900
Recombinant human G-CSF protein (ab167700) | Abcam
Active Recombinant human G-CSF protein is a HEK 293 Protein fragment 31 to 204 aa range, | 95% purity, | 1.000 Eu/µg endotoxin level and validated in FuncS, SDS-PAGE. The bio-activity was determined …
Recombinant Human G-CSF protein (ab54137) | Abcam
Buy our Recombinant Human G-CSF protein. Ab54137 is a protein fragment produced in Escherichia coli and has been validated in SDS-PAGE. Abcam provides free…
Bacterial artificial chromosomes improve recombinant protein production in mammalian cells | BMC Biotechnology | Full Text
Recombinant protein production in mammalian cells is an important topic in biotechnology . One of the critical steps in the production of recombinant proteins is the isolation of stable single cell clones expressing high levels of the protein of interest. Commonly, this is achieved by random genomic integration of a vector containing a promoter, a gene of interest and a selectable marker. Although this method is simple and straight forward, it lacks of reproducibility. Expression from such vectors is substantially influenced by the surrounding chromatin to the integration site and tends to be silenced over time. This makes the selection of suitable clones a tedious and time consuming procedure . Several strategies have been developed to overcome the positional effects of the adjacent chromatin. For example, anti-repressor elements flanking the vectors  have been used or vectors have been integrated specifically into chromosomal loci with open chromatin . Ideally, a vector for ...
Heterogeneity in recombinant protein production - DTU Orbit
etc. the cell physiology is affected. Cells are stressed, and this may severely affect growth, by-product accumulation, biomass yield and recombinant product yield. The stress caused by exposure to divergent microenvironments, genetic differences of individual cells, differing cell cycle stage and cell age, all contribute to make a population in a fermenter heterogeneous, resulting in cell-to-cell variation in physiological parameters of the microbial culture. Our study aims at investigating how population heterogeneity and recombinant protein production is affected by environmental gradients in bioreactors. For this purpose, a Saccharomyces cerevisiae strain, that functions as a protein production reporter, has been developed. A heterologous protein has been tagged with a fluorescent protein providing a way to measure the amount of heterologous protein produced by the cells on single cell level. Gradients are simulated in small bioreactors and the population heterogeneity can be visualised by ...
Novel cell lines derived from transgenic mice expressing recombinant human proteins | SpringerLink
We have used transgenic mouse technology to establish immortalized hepatoma cell lines stably secreting heterologous proteins, such as human α1-antitrypsin and human factor IX. Hepatocyte-specific...
Impact of protein uptake and degradation on recombinant protein secretion in yeast - Danish National Research Database-Den...
Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream purification, compared to other systems that require complex media. As such, engineering S. cerevisiae to improve titers has been then the subject of significant attention, but the majority of previous efforts have been focused on improving protein synthesis. Here, we characterize the protein uptake and degradation pathways of S. cerevisiae to better understand its impact on protein secretion titers. We do find that S. cerevisiae can consume significant (in the range of 1 g/L/day) quantities of whole proteins. Characterizing the physiological state and combining metabolomics and transcriptomics, we identify metabolic and regulatory markers that are consistent with uptake of whole ...
List of recombinant proteins - Wikipedia
The following is a list of notable proteins that are generated from recombinant DNA, using biomolecular engineering, focusing on those that are used in human and veterinary medicine. In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. The prefix rh for recombinant human appears less and less in the literature. A much larger number of recombinant proteins is used in the research laboratory. These include both commercially available proteins (for example most of the enzymes used in the molecular biology laboratory), and those that are generated in the course specific research projects. Human growth hormone (rHGH): Humatrope from Lilly and Serostim from Serono replaced cadaver harvested human growth hormone human insulin (BHI): Humulin from Lilly and Novolin[disambiguation needed] from Novo Nordisk among others largely replaced bovine and porcine insulin for human therapy. Some prefer to continue using the animal-sourced preparations, as ...
Recombinant Protein Expression Now Available at Maine Biotechnology Services
Portland, Maine (PRWEB) June 18, 2014 -- Maine Biotechnology Services is announcing the addition of Bacterial Recombinant Protein Expression and Purification
Three Enzyme Expression Systems Provided by Creative Enzymes | New York Chronicle
Every kind of biological life process, from small to large, multiply, life and death, metabolism, is related to enzymes. If there is no catalysis of enzymes, the most basic food digestion in life and oxygen breathing can not be carried out. In fact, the various reactions occurring in the body are almost always carried out by the enzyme. It can be said that there is no enzyme, there is no life.. Here are three enzyme catalytic systems, which let us better understand the function and function of the enzyme from Creative Enzymes.. E. coli Enzyme Expression System. To date, a variety of prokaryotic and eukaryotic expression systems have been developed to produce recombinant proteins. Compared with other systems, Escherichia coli expression system has the advantages of clear genetic background, easy operation, large-scale fermentation culture, and is the most commonly used expression system at this stage. However, in the process of exogenous gene expression, there may be problems such as low ...
Inflammatory properties of recombinant tumor necrosis factor in rabbit skin in vivo. | Journal of Experimental Medicine |...
We have investigated the ability of recombinant TNF (mouse and human) to produce acute inflammatory lesions in an established experimental model of inflammation
Global transcriptional analysis of an Escherichia coli recombinant protein process during hypoxia and hyperoxia
Both exposure to oxygen and recombinant protein production are known to have adverse effects on microbial fermentation, including increased proteolytic and oxidative damage to the product. In an effort to characterize the effects of these stresses on the cell, DNA microarrays were used to monitor global gene expression of E. coli producing recombinant human αl-antitrypsin (α₁AT) during exposure to defined aeration conditions. Recombinant α₁AT has been shown to undergo oxygen-dependent degradation during production in E. coli, due in part to activation of the heat-shock response. The goal of this work is to better understand the effects of oxygen in order to improve this recombinant protein production process. In order to study the effects of oxygen extremes, global expression analysis was performed on α₁AT-producing cultures exposed to pure nitrogen, air, and pure oxygen. The most notable effects of oxygen exposure were those of superoxide. This reactive oxygen species is generated ...
A new generation of recombinant polypeptides combines multiple protein domains for effective antimicrobial activity
Background Although most of antimicrobial peptides (AMPs), being relatively short, are produced by chemical synthesis, several AMPs have been produced using recombinant technology. However, AMPs could be cytotoxic to the producer cell, and if small they can be easily degraded. The objective of this study was to produce a multidomain antimicrobial protein based on recombinant protein nanoclusters to increase the yield, stability and effectivity. Results A single antimicrobial polypeptide JAMF1 that combines three functional domains based on human α-defensin-5, human XII-A secreted phospholipase A2 (sPLA2), and a gelsolin-based bacterial-binding domain along with two aggregation-seeding domains based on leucine zippers was successfully produced with no toxic effects for the producer cell and mainly in a nanocluster structure. Both, the nanocluster and solubilized format of the protein showed a clear antimicrobial effect against a broad spectrum of Gram-negative and Gram-positive bacteria, ...
EGFR recombinant monoclonal antibody, clone 7A7 - (RAB00103) - Products - Abnova
Recombinant rabbit monoclonal antibody raised against EGFR. Original antibody is raised against recombinant protein corresponding to extracellular domain of mouse EGFR. (RAB00103) - Products - Abnova
HSP90B1 recombinant monoclonal antibody, clone HSP90B1/3168R - (RAB00648) - Products - Abnova
Recombinant rabbit monoclonal antibody raised against full length human HSP90B1. Recombinant protein corresponding to full-length human HSP90B1. (RAB00648) - Products - Abnova
A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli.
ABSTRACT: BACKGROUND: Recombinant protein production is a process of great industrial interest, with products that range from pharmaceuticals to bi...
Plantae | Cell-free membrane protein expression system enables functional characterization of receptor-like protein kinase...
Membrane proteins are some of the most interesting cellular proteins, serving as sensors and transducers of diverse signals, yet they also are the most…
Making Recombinant Proteins - Protein Expression and Purification - BioForum
Making Recombinant Proteins - posted in Protein Expression and Purification: My boss wants me to make a recombinant protein and this is something that I have never done before. The protein that I want to make is Recombinant Human Bone Morphogenetic Protein-7 and the product sheet of this compound where we first purchased the protein states that it is a 28.8 kDa homodimer, each subunit contains 116 amino acid residues (corresponding to amino acid residues 316 to 431 of the full-length...
Trends in recombinant protein use in animal production | Microbial Cell Factories | Full Text
The use of genetic and protein engineering techniques have led to a significant progress in animal production and it is starting to have a commercial impact in this field. Nowadays it is possible to design tailor-made sequences of enzymes, which in some cases combine specific properties of different enzymes in one molecule to obtain an optimal functional protein . On the other hand, this technology allows the production of recombinant hormones through cost-effective processes using microbial cells as production hosts. In addition to this, novel strategies such as those based on passive immunization are gaining ground due to the broad range of possibilities that recombinant protein production offers. In this context, although important efforts have been done toward the minimization of recombinant protein production costs, currently, much remains still to be achieved. Cost effectiveness is particularly important in the context of animal production, where marginal returns are tight. Currently, ...
US Patent # 8,067,198. Protein expression system - Patents.com
The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric
List of recombinant proteins
The prefix "rh" for "recombinant human" appears less and less in the literature. A much larger number of recombinant proteins ... Protein production Gene expression Protein purification Host cell protein Young CL, Britton ZT, Robinson AS (May 2012). " ... For the studies of individual ribosomal proteins, the use of proteins that are produced and purified from recombinant sources ... and characterization of recombinant 60S ribosomal acidic proteins from Saccharomyces cerevisiae". Protein Expression and ...
NGR-hTNF (antitumor recombinant protein)
NGR-hTNF is a recombinant protein derived from the fusion between peptide CNGRCG and human tumor necrosis factor alpha (TNFα). ...
Recombinant subunit vaccine
Wingfield PT (April 2015). "Overview of the purification of recombinant proteins". Current Protocols in Protein Science. 80 (1 ... recombinant protein aggregation). For mammalian cells, synthesised proteins were reported to be secreted into chemically ... The baculovirus-insect cell expression system has the ability to express a variety of recombinant proteins at high levels and ... The vaccines contain purified VLP of the major capsid L1 protein produced by recombinant Saccharomyces cerevisiae. It has been ...
Weissmann, Charles (2001). "Recombinant interferon - the 20th anniversary". In Buckel, Peter (ed.). Recombinant Protein Drugs. ... It is a recombinant form of the protein Interferon alpha-2 that was originally sequenced and produced recombinantly in E. coli ... It was also produced in 1986 in recombinant human form, in the Center for Genetic Engineering and Biotechnology of Havana, Cuba ... but the period of usefulness is limited by the production of antibodies against this foreign protein. Interferon ...
... s are recombinant proteins. As they are manufactured using recombinant bacterial production processes, the batch-to- ... Affimer proteins display two peptide loops that can all be randomized to bind to desired target proteins, in a similar manner ... Affimers that inhibit protein-protein interactions can be produced with the potential to express these inhibitors in mammalian ... Affimer molecules are small proteins that bind to target proteins with affinity in the nanomolar range. These engineered non- ...
"Recombinant Human SH2D3A Protein". potassium-channel. Potassium Channel. 1 August 2017. Retrieved 18 September 2017. "SH2D3A ... SH2 domain-containing protein 3A is a protein that in humans is encoded by the SH2D3A gene. The protein may be useful in ... Lu Y, Brush J, Stewart TA (Apr 1999). "NSP1 defines a novel family of adaptor proteins linking integrin and tyrosine kinase ... "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane ...
"SURF2 Protein Human Recombinant , Surfeit 2 Antigen , ProSpec". v t e (Articles with short description, Short description ... SURF2 is a protein which in humans is encoded by the SURF2 gene.SURF2 is a member of the surfeit gene family. The SURF2 ... matches Wikidata, Protein pages needing a picture, Genes on human chromosome 9, Human gene pages with Wikidata item, All stub ...
"Nesfatin-1 Recombinant Protein". Archived from the original on 11 April 2013. Retrieved 21 March 2013. Pan, Weihong; Hsuchou, ... Nesfatin-1 is a polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human ... nesfatin-1+protein,+rat at the US National Library of Medicine Medical Subject Headings (MeSH) (Articles with short description ... In addition, the protein stimulated insulin secretion from the pancreatic beta cells of both rats and mice. ...
The applications are increasing because recombinant DNA is very useful in creating proteins that are identical when exposed ... Recombinant Proteins from Plants. Methods in Molecular Biology. Vol. 483. pp. 103-134. doi:10.1007/978-1-59745-407-0_7. ISBN ... stable proteins by capture on commercially produced protein A resins. Production of antibodies in whole transgenic plants, such ... A recombinant DNA is an artificial DNA that is created by combining two or more sequences that would not normally come together ...
Eosinophil cationic protein
Rosenberg HF (April 1995). "Recombinant human eosinophil cationic protein. Ribonuclease activity is not essential for ... Eosinophil cationic protein (ECP) also known as ribonuclease 3 is a basic protein located in the eosinophil primary matrix. In ... Eosinophil+Cationic+Protein at the US National Library of Medicine Medical Subject Headings (MeSH) RNASE3+protein,+human at the ... Both proteins possess neurotoxic, helmintho-toxic, and ribonucleo-lytic activities. Eosinophil cationic protein is localized to ...
Characterization of the recombinant proteins". J. Biol. Chem. 276 (20): 17022-9. doi:10.1074/jbc.M100623200. PMID 11279105. ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode: ... identification in a genetic screen and effects on catalysis by the recombinant proteins". Adv. Enzyme Regul. 44: 37-49. doi: ... Similarity to an E. coli protein suggests that this enzyme may be a subunit of DNA polymerase III, which does not have ...
"Recombinant human Cardiotrophin 1 protein (ab9838)". www.abcam.com. Retrieved 2017-08-09. cardiotrophin+1 at the US National ... It is a cardiac hypertrophic factor of 21.5 kDa and a protein member of the IL-6 cytokine family. CT-1 is associated with the ... The protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor ... v t e (Articles with short description, Short description matches Wikidata, Protein pages needing a picture, Genes on human ...
Gene expression Single-cell protein Protein purification Precision fermentation Host cell protein List of recombinant proteins ... This is followed by selection and screening of recombinant clones. The non-lytic system has been used to give higher protein ... Cregg JM, Cereghino JL, Shi J, Higgins DR (September 2000). "Recombinant protein expression in Pichia pastoris". Molecular ... McCarroll L, King LA (October 1997). "Stable insect cell cultures for recombinant protein production". Current Opinion in ...
De Schutter, K., Lin, Y., Tiels, P. (2009). "Genome sequence of the recombinant protein production host Pichia pastoris". ... As the protein yield from expression system in a microbe is roughly equal to the product of the proteins produced per cell, ... 2000). "Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy‐chain ... As some proteins require chaperonin for proper folding, Pichia is unable to produce a number of proteins, since P. pastoris ...
This study on recombinant resilin has led to several years of research on the use of resilin like proteins for several ... In 2005, a recombinant form of the resilin protein of the fly Drosophila melanogaster was synthesized by expressing a part of ... Tjin MS, Low P, Fong E (2014-08-01). "Recombinant elastomeric protein biopolymers: progress and prospects". Polymer Journal. 46 ... elastin: a vertebrate protein Deming T (2012). Peptide-Based Materials. Springer Publishing. Neurath H (1966). The Proteins ...
... is a recombinant protein subunit. Vaxine began work on a COVID-19 vaccine in January 2020. After developing a number ... COVAX-19 is a recombinant protein-based COVID-19 vaccine developed by South Australian-based biotech company Vaxine. It is ... "recombinant protein-based vaccine". A phase 1 human trial started in June 2020. The phase 1 trial involved 40 participants, 30 ... Placebo-controlled Trial to Evaluate the Efficacy and Safety of an Adjuvanted Recombinant SARS-CoV-2 Spike (S) Protein Subunit ...
Strasser R, Altmann F, Steinkellner H (December 2014). "Controlled glycosylation of plant-produced recombinant proteins". ... A chaperone protein (calnexin/calreticulin) binds to the unfolded or partially folded protein to assist protein folding. The ... The proteins produced in these expression hosts are often not identical to human protein and thus, cause immunogenic reactions ... This initial trimming step is thought to act as a quality control step in the ER to monitor protein folding. Once the protein ...
"Coagulation Factor IX (Recombinant), Fc Fusion Protein". Drug Information Portal. U.S. National Library of Medicine. " ... The factor IX protein is composed of four protein domains: the Gla domain, two tandem copies of the EGF domain and a C-terminal ... Deficiency of this protein causes haemophilia B. It was discovered in 1952 after a young boy named Stephen Christmas was found ... A structure of the two EGF domains and the trypsin-like domain was determined for the pig protein. The structure of the Gla ...
Sanofi-GSK COVID-19 vaccine
The Sanofi-GSK COVID‑19 vaccine is a recombinant protein subunit vaccine containing the SARS-CoV-2 spike protein, which is ... "Study of Recombinant Protein Vaccine with Adjuvant against COVID-19 in Adults 18 Years of Age and Older". pactr.samrc.ac.za. ... It uses a recombinant protein-based technology from Sanofi and GSK's pandemic technology. The companies claimed to be able to ... "Study of Recombinant Protein Vaccine With Adjuvant Against COVID-19 in Adults 18 Years of Age and Older (VAT00002)". ...
Periodic counter-current chromatography
"Integrated continuous production of recombinant therapeutic proteins". Biotechnology and Bioengineering. 109 (12): 3018-3029. ... When purifying antibodies, Protein A is used as affinity matrix. However, periodic counter-current processes can be applied to ... A novel cyclic process for protein A affinity chromatography". Journal of Chromatography A. 1389: 85-95. doi:10.1016/j.chroma. ... "Comparison of batch and continuous multi-column protein A capture processes by optimal design". Biotechnology Journal. 11 (7): ...
These proteins must be fermented much like in recombinant protein production, alcohol brewing and the generation of many plant- ... Wingfield, Paul T. (1 April 2015). "Overview of the Purification of Recombinant Proteins". Current Protocols in Protein Science ... Proteins are coded for by specific genes, the genes coding for the protein of interest are synthesized into a plasmid-a closed ... Synthetic kinds of collagen have been produced through recombinant protein production-collagen type II and III, tropoelastin ...
Characterization of cDNAs and recombinant fusion proteins". Eur. J. Biochem. 259 (3): 618-25. doi:10.1046/j.1432-1327.1999. ... Plasminogen-related protein B is a protein that in humans is encoded by the PLGLB2 gene. GRCh38: Ensembl release 89: ... v t e (Genes on human chromosome 2, All stub articles, Protein stubs). ...
Gifre L, Arís A, Bach À, Garcia-Fruitós E (March 2017). "Trends in recombinant protein use in animal production". Microbial ... Recombinant phytases are added commonly in agriculture to animal feed of monogastric animals to enhance the feed's nutrient ... β-propeller phytases (BPPs) are a group of enzymes (i.e. protein superfamily) with a round beta-propeller structure. BPPs are ... Chen C, Cheng K, Ko T, Guo R (2015-04-01). "Current Progresses in Phytase Research: Three-Dimensional Structure and Protein ...
... is a recombinant human coagulation factor VIII, Fc fusion protein (rFVIIIFc). It is produced by recombinant ... "Eloctate (antihemophilic factor- recombinant, fc fusion protein kit". DailyMed. 4 August 2020. Retrieved 3 September 2020. " ... Recombinant proteins, All stub articles, Blood and blood forming organ drug stubs). ...
Peptidoglycan recognition protein 1
"Recombinant Human Peptidoglycan Recognition Proteins Reveal Antichlamydial Activity". Infection and Immunity. 84 (7): 2124-2130 ... Peptidoglycan recognition protein Peptidoglycan recognition protein 2 Peptidoglycan recognition protein 3 Peptidoglycan ... peptidoglycan recognition protein 1), PGLYRP2 (peptidoglycan recognition protein 2), PGLYRP3 (peptidoglycan recognition protein ... January 2004). "Peptidoglycan recognition protein tag7 forms a cytotoxic complex with heat shock protein 70 in solution and in ...
R. S.-C. Su, J. N. Renner, and J. C. Liu, "Synthesis and characterization of recombinant abductin-based proteins," ... The abductin-based protein was cytocompatible, and cells spread slowly when first seeded on the abductin-based protein. A LIVE/ ... Synthesis and Characterization of Recombinant Abductin-Based Proteins. Biomacromolecules, [online] 14(12), pp.4301-4308. U.S. ... after being cultured for two days on the abductin-based protein. Initial cell spreading on the abductin-based protein was ...
doi:10.1016/S0960-9822(03)00176-3. (Protein methods, Recombinant proteins, Cell imaging, Fluorescent proteins). ... It consists of a pH-sensitive form of green fluorescent protein (GFP) fused to the luminal side of a vesicle-associated ... Synapto-pHluorin sometimes consists of yellow fluorescent protein (YFP) to monitor the cytoplasm because its pKa is higher than ... Ashby, Michael C.; Ibaraki, Kyoko; Henley, Jeremy M. (May 2004). "It's green outside: tracking cell surface proteins with pH- ...
Peptidoglycan recognition protein 2
Recombinant and native human PGLYRP2 proteins were then further shown to be identical with the previously identified and ... Peptidoglycan recognition protein Peptidoglycan recognition protein 1 Peptidoglycan recognition protein 3 Peptidoglycan ... peptidoglycan recognition protein 1), PGLYRP2 (peptidoglycan recognition protein 2), PGLYRP3 (peptidoglycan recognition protein ... "Recombinant Human Peptidoglycan Recognition Proteins Reveal Antichlamydial Activity". Infection and Immunity. 84 (7): 2124-2130 ...
The products of pharming are recombinant proteins or their metabolic products. Recombinant proteins are most commonly produced ... Blood, for instance, as of 2012 cannot store high levels of stable recombinant proteins, and biologically active proteins in ... Many proteins of interest are too complex to be made by microbial systems or by protein synthesis. These proteins are currently ... substantially reduce the burden of protein purification in preparing recombinant proteins for medical use. In addition, both ...
Production of heterologous proteins by fed-batch cultures of recombinant microorganisms have been extensively studied. The ... Eds). Recombinant Protein Production with prokaryotic and eukaryotic cells. A comparative view on host physiology. 2001, Kluwer ... L. Yee, Harvey W. Blanch: Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli. Bio/ ... Neubauer P, Winter J: Expression and fermentation strategies for recombinant protein production in Escherichia coli. In: Merten ...
... is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that ... "Recombinant human Fab fragments neutralize human type 1 immunodeficiency virus in vitro". Proceedings of the National Academy ... a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on ... characterize small molecules-protein interactions and map protein-protein interactions. Users can use three dimensional ...
... in combination with inactivated HIV proteins. The novel vaccine consists of a recombinant DNA vaccine co-expressing human GM- ... GeoVax technology approach uses recombinant DNA or recombinant viruses to produce virus-like particles (VLPs) in the person ... The DDMM regimen consists of priming with two doses of the pGA2/JS7 recombinant DNA vaccine and boosting with two doses of VA/ ... HIV62B recombinant MVA vaccine. The MMM regimen consists of priming and boosting with a total of three doses of the recombinant ...
Murphy CI, Lennick M, Lehar SM, Beltz GA, Young E (Oct 1990). "Temporal expression of HIV-1 envelope proteins in baculovirus- ... "Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human ... Montefiori DC, Robinson WE, Mitchell WM (Dec 1988). "Role of protein N-glycosylation in pathogenesis of human immunodeficiency ... v t e (Genes on human chromosome 15, All stub articles, Protein stubs). ...
Endless Forms Most Beautiful (book)
... of their proteins, a figure that rises to 95% for humans and mice. Thus we can't exclude protein-sequence evolution as an ... That task was finally undertaken at the end of the 20th century with the arrival of recombinant DNA technology, when biologists ... Most of the changes are in genetic control, not in proteins. 11. Endless Forms Most Beautiful Carroll concludes by revisiting ... which do not code for structural proteins (such as enzymes), control embryonic development. In turn, these regulatory genes ...
Institute of Microbial Technology
Patented natural, recombinant and clot specific Streptokinase as a vital lifesaving drug. The institute offers Ph.D. jointly ... equipment for protein and DNA analysis, a library with around 64,000 references books, microscopy equipment, and databases for ... protein design and engineering, fermentation science, microbial physiology and genetics, yeast biology, bioinformatics, ...
Fragmentation (cell biology)
The basic scheme for this can be summarized as follows: Vector + DNA Fragment ↓ Recombinant DNA ↓ Replication of recombinant ... Genes involved in controlling cell death encode proteins with three distinct functions: "Killer" proteins are required for a ... After a single recombinant DNA molecule (composed of a vector plus an inserted DNA fragment) is introduced into a host cell, ... Here, DNA fragmentation is a molecular genetic technique that permits researchers to use recombinant DNA technology to prepare ...
Some viruses can encode proteins that bind to double-stranded RNA (dsRNA) to prevent the activity of RNA-dependent protein ... Patients with recurrent melanomas receive recombinant IFN-α2b. Both hepatitis B and hepatitis C are treated with IFN-α, often ... the E7 protein of Human papillomavirus (HPV), and the B18R protein of vaccinia virus. Reducing IFN-α activity may prevent ... phosphorylates ribosomal protein s6, which is involved in protein synthesis; and phosphorylates a translational repressor ...
... increase in copy number is useful for genetic engineering applications to increase the production yield of recombinant protein ... PFF1 consists of an origin of replication, oriV, an origin of transfer, oriT, a gene coding for plasmid replication proteins, ... 4486-4491 Kolatka K, Witosinska M, Pierechod M, Konieczny I.: "Bacterial partitioning proteins affect the subcellular location ... the TrfA protein, binds to and activates oriV. In Escherichia coli, replication proceeds unidirectionally from oriV after ...
There is also a group of tracers that consist of protein products that can be taken up by the cell and transported across the ... Chamberlin NL, Du B, de Lacalle S, Saper CB (May 1998). "Recombinant adeno-associated virus vector: use for transgene ... virus or protein can be locally injected, after which it is allowed to be transported anterogradely. Viral tracers can cross ... green fluorescent protein, lipophylic dyes or radioactively tagged amino acids) into the brain. These molecules are absorbed ...
Bio-pharmaceuticals developed by the company include Pichia-derived recombinant human insulin and insulin analogs for diabetes ... matrix proteins, and biosimilars. Biocon's major areas of research now include cancer, diabetes, and other auto-immune diseases ...
This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to ... Appiah-Opong R, Commandeur JN, van Vugt-Lussenburg B, Vermeulen NP (June 2007). "Inhibition of human recombinant cytochrome ... The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of ...
"Immunization of Experimental Dogs With Salivary Proteins From Lutzomyia Longipalpis, Using DNA and Recombinant Canarypox Virus ... The strong immune reaction in response to exposure to the salivary protein indicates the protein's potential use in the field ... The strong immune reaction in response to exposure to the salivary protein indicates the protein's potential use in the field ... Blood is rich in proteins, consisting mainly of hemoglobin (Hb), which accounts for approximately 60% of the blood protein ...
Recombinant proteins, All stub articles, Antineoplastic and immunomodulating drug stubs). ... Ancestim is a recombinant methionyl human stem cell factor, branded by Amgen as StemGen. It was developed by Amgen and sold to ... The protein has an amino acid sequence that is identical to the natural sequence predicted from human DNA sequence analysis, ... It is a 166 amino acid protein produced by E. coli bacteria into which a gene has been inserted for soluble human stem cell ...
... and activator protein 1 signaling pathways". Journal of Immunology. 172 (4): 2341-2351. doi:10.4049/jimmunol.172.4.2341. ISSN ... "An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors". Proceedings of the ...
These conditions are associated with either defective intracellular protein binding (for the second time) or disturbed ... Proper Assay Conditions to Estimate Enzyme Kinetics with Recombinant UGT1A1". Drug Metabolism and Disposition. 38 (11): 1907- ... or defective intracellular protein binding. In similar fashion, the conjugated hyperbilirubinemia emerges in case the ... impaired biliary excretion of bilirubin glucuronide is due to a mutation in the canalicular multiple drug-resistance protein 2 ...
Their current goal is expression of orally bioavailable recombinant proteins in tobacco and lettuce chloroplasts. Thomas Alva ... of the total leaf protein. Importantly, the insecticidal protein could be translated from the bacterial AU-rich mRNA, while for ... The Maliga lab characterised plastid promoters in vivo and in vitro, and identified proteins that are parts of the plastid PEP ... "Amplification of a Chimeric Bacillus Gene in Chloroplasts Leads to an Extraordinary Level of an Insecticidal Protein in Tobacco ...
Proc Natl Acad Sci USA 85:8506-8510 (1988) v-cbl, a new oncogene from a dual-recombinant murine retrovirus that induced early B ... Blood 111: 1946-1950 (2008) Liar, a novel Lyn-binding nuclear/cytoplasmic shuttling protein that influences erythropoietin- ... Nature 507:462-470 (2014) Complementing tissue characterisation by integrating transcriptome profiling from the Human Protein ...
However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than ... Stopped-flow kinetic studies with a substrate analog and recombinant TXA synthase revealed that substrate binding occurs in two ... First, there is a fast initial binding to the protein and then a subsequent ligation to the heme iron. In the first step of the ... The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of ...
The secreted protein consists of 133`amino acids (mouse Lect2 consists of two varieties a typical 151 amino acid protein and an ... LECT2 in human hepatocytes revealed by immunochemical studies using polyclonal and monoclonal antibodies to a recombinant LECT2 ... The protein was detected in and purified from cultures of Phytohaemagglutinin-activated human T-cell leukemia SKW-3 cells. ... LECT2 protein is widely expressed in vascular tissues, smooth muscle cells, adipocytes, cerebral neurons, apical squamous ...
Goodpasture antibodies recognize recombinant proteins representing the autoantigen and one of its alternative forms". Eur. J. ... The enzyme belongs to the serine/threonine protein kinase family and to the Ca2+/calmodulin-dependent protein kinase subfamily ... "KN-93 inhibition of G protein signaling is independent of the ability of Ca2+/calmodulin-dependent protein kinase II to ... Calcium/calmodulin-dependent protein kinase type II beta chain is an enzyme that in humans is encoded by the CAMK2B gene. ...
The recombinant vaccine against Lyme disease, based on the outer surface protein A (OspA) of B. burgdorferi with aluminum ... Canine Recombinant Lyme, formulated by Merial, generates antibodies against the OspA protein so a tick feeding on a vaccinated ... Within the tick midgut, the Borrelia's outer surface protein A (OspA) binds to the tick receptor for OspA, known as TROSPA. ... After the bacteria migrate from the midgut to the salivary glands, OspC binds to Salp15, a tick salivary protein that appears ...
Heart-type fatty acid binding protein
"Three-dimensional structure of recombinant human muscle fatty acid-binding protein". The Journal of Biological Chemistry. 267 ( ... Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is ... Heart-type Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ...
Appl H, Holzammer T, Dove S, Haen E, Strasser A, Seifert R (February 2012). "Interactions of recombinant human histamine H1R, ... Haemodialysis is not recommended due to the high degree of protein binding with doxepin. ECG monitoring is recommended for ... Doxepin is widely distributed throughout the body and is approximately 80% plasma protein-bound, specifically to albumin and α1 ... Virtanen R, Iisalo E, Irjala K (August 1982). "Protein binding of doxepin and desmethyldoxepin". Acta Pharmacologica et ...
Metabotropic glutamate receptor
Proteins called PDZ proteins frequently anchor mGluRs near enough to NMDARs to modulate their activity. It has been suggested ... L-quisqualic acid and recombinant receptors" (subscription required). Zeitschrift für Naturforschung C. 57 (3-4): 348-55. doi: ... They are members of the group C family of G-protein-coupled receptors, or GPCRs. Like all glutamate receptors, mGluRs bind with ... Wang Y, Small DL, Stanimirovic DB, Morley P, Durkin JP (October 1997). "AMPA receptor-mediated regulation of a Gi-protein in ...
Secondly, another insert of 20 amino acids in the 10th spectrin repeat, termed SH3i+, contains protein kinase A and protein ... "Mapping the human erythrocyte beta-spectrin dimer initiation site using recombinant peptides and correlation of its phasing ... Herrmann H, Wiche G (1987). "Plectin and IFAP-300K are homologous proteins binding to microtubule-associated proteins 1 and 2 ... Ankyrin repeats of the multidomain Shank protein family interact with the cytoskeletal protein alpha-fodrin". J. Biol. Chem. ...
It is thought this may be due to increased synthesis of receptor proteins. Flumazenil was found to be more effective than ... "Chronic treatment with flumazenil enhances binding sites for convulsants at recombinant alpha(1)beta(2)gamma(2S) GABA(A) ... "The role of transcriptional and translational mechanisms in flumazenil-induced up-regulation of recombinant GABA(A) receptors ... "Chronic exposure of cells expressing recombinant GABAA receptors to benzodiazepine antagonist flumazenil enhances the maximum ...
A recombinant form of c-Myc called Omomyc in which four residues are mutated has been produced. Omomyc heterodimers with c-Myc ... The protein contains basic helix-loop-helix (bHLH) structural motif. This gene is a proto-oncogene and encodes a nuclear ... The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA ... MYC proto-oncogene, bHLH transcription factor is a protein that in humans is encoded by the MYC gene which is a member of the ...
... quantification and purification of recombinant protein. This patented peptide sequence is composed of eighteen hydrophilic ... This short peptide does not adopt any significant homology to any existing proteins found in nature. This synthetic NE peptide ... and affinity purification of NE fusion proteins (i.e. affinity column). The DNA sequence of NE-tag is as follows: 5' ACC AAA ... This is advantageous to offer stringent specificity to the NE-tagged proteins, which are readily to be detected, quantitated, ...
Recombinant inbred strain
... and the electrophoretic mobility of proteins. Somewhat larger families of recombinant inbred strains were generated ... A recombinant inbred strain or recombinant inbred line (RIL) is an organism with chromosomes that incorporate an essentially ... The origins and history of recombinant inbred strains are described by Crow. While the potential utility of recombinant inbred ... Recombinant inbred strains or lines were first developed using inbred strains of mice but are now used to study a wide range of ...
Peptides that were translocated into the cell membrane then exert a secondary effect by inhibiting protein synthesis. It is ... "Recombinant expression, synthesis, purification, and solution structure of arenicin". Biochemical and Biophysical Research ... Webarchive template wayback links, Orphaned articles from October 2013, All orphaned articles, Protein pages needing a picture ...
Recombinant Protein Expression (Baculovirus System)
We express proteins of interest(POIs) in insect cells using recombinant baculovirus. Protein production is performed on a small ... Overexpression of recombinant proteins in any of three systems. E.coli. We provide consultation for all projects and can assist ... Rapid production of recombinant proteins in 5-7 days. *High density cultures of Expi293F cells that result in more expressing ... atc-protein[email protected] Baculovirus and Antibody Production - Karen Moberg. [email protected] Protein Expression Purification - ...
Recombinant Staphylococcus aureus Chemotaxis inhibitory protein (chp) | CSB-EP746696SKV | Cusabio
Recombinant Staphylococcus aureus Chemotaxis inhibitory protein (chp) from Cusabio. Cat Number: CSB-EP746696SKV. USA, UK & ... Recombinant Staphylococcus aureus Chemotaxis inhibitory protein (chp) , CSB-EP746696SKV. (No reviews yet) Write a Review Write ... Recombinant Staphylococcus aureus Acyl carrier protein (acpP) , CSB-EP015636SKX Cusabio Staphylococcus aureus Recombinants ... Recombinant Staphylococcus aureus Acyl carrier protein (acpP) , CSB-EP015636SKX Cusabio Staphylococcus aureus Recombinants ...
Bacillus subtilis Malate Dehydrogenase (MDH) Protein (His tag), Recombinant | ABIN1458314
Konjugiert mit His tag.Bestellen Sie Protein ABIN1458314 online. ... CG5889 Protein, Dmel\\CG5889 Protein, MDH Protein, Mdh Protein ... Recombinant Bacillus subtilis Malate Dehydrogenase Protein exprimiert von Yeast. ... mdh Protein, APH_RS02635 Protein, ANI_1_12134 Protein, AOR_1_26114 Protein, PMI_RS16925 Protein, mdhA Protein, mdh1 Protein ... 0230186 Protein, DDB_0188779 Protein, DDB_0230186 Protein, Malic enzyme b Protein, oxidoreductase, putative Protein, malate ...
Gene Delivery into Plant Cells for Recombinant Protein Production
... the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein ... facilitating the adoption of plant transient expression systems for manufacturing recombinant proteins with a broad range of ... solution to overcome the challenge of transgene delivery into plant cells for large-scale manufacturing of recombinant proteins ... The discussion of agroinfiltration vectors focuses on their applications for producing complex and heteromultimeric proteins ...
Preparation of Antibodies against Soluble Recombinant Dengue E Proteins Fused with Glutathione's Transferase.
Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF 1131-ER-050: R&D Systems
Her4 protein (1131-ER) is manufactured by R&D Systems. Reproducible results in bioactivity assays. Learn More... ... as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, ... Recombinant Proteins. Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein 377-HB ... Reviews for Recombinant Human ErbB4/Her4 Fc Chimera Protein, CF. There are currently no reviews for this product. Be the first ...
KIT [D820E] Recombinant Human Protein
Disseminated Intravascular Coagulation (DIC) Medication: Anticoagulants, Hematologic, Recombinant Human Activated Protein C,...
Consumption and subsequent exhaustion of coagulation proteins and pl... ... Recombinant Human Activated Protein C. Class Summary. Recombinant human APC inhibits factors Va and VIIIa of the coagulation ... FFP contains coagulation factors, as well as protein C and protein S. It can be administered either via 2 large-bore peripheral ... Compared patterns of antithrombin III, protein C, and protein S deficiencies. Chest. 1992 Mar. 101(3):816-23. [QxMD MEDLINE ...
Recombinant Mouse Adiponectin protein (ab285685) | Abcam
Recombinant Mouse Adiponectin protein is an Escherichia coli Full length protein, , 90% purity, , 1.000 Eu/µg endotoxin level ... SDS-PAGE (4-20%) of Recombinant Mouse Adiponectin: Recombinant Protein loaded under reducing conditions and stained with ... Proteins and Peptides. By product type. Proteomics tools. Agonists, activators, antagonists and inhibitors. Cell lines and ... The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. 38:D142-D148 (2010) . ...
Promega QuantiLum Recombinant Luciferase:Protein Analysis Reagents:Protein | Fisher Scientific
QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis ... The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. ... QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis ...
mCherry Escherichia coli Recombinant Protein - TP790040 | OriGene
Purified fluorescent protein mCherry, with N-terminal HIS tag, expressed in E. coli, 250ug ... Video tutorial: Why protein expression host matters * Webinar Video: Overexpression lysate as positive controls for Western ... Protein Sequence (showhide) MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL ... Purified fluorescent protein mCherry, with N-terminal HIS tag, expressed in E. coli, 250ug ...
Transgenic silkworms that weave recombinant proteins into silk cocoons | Semantic Scholar
This review focuses on the expression of recombinant protein in the MSG of transgenic silkworms. The recombinant protein ... which is a valuable tool for the mass production of therapeutic and industrially relevant recombinant proteins. As a result of ... By controlling the expressed regions of the recombinant protein gene in the silk gland, we were able to control the ... transgenic silkworms were generated that synthesized recombinant proteins in the silk gland and secreted them into the silk ...
THBD Human Recombinant | Thrombomodulin Protein | ProSpec
THBD Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 500 amino acids ... For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).. Avoid multiple freeze-thaw cycles. ... THBD Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 500 amino acids ... THBD protein solution (0.5mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 10% glycerol. ...
Recombinant Human MAF1 His Protein (NBP1-72440): Novus Biologicals
Recombinant Human MAF1 His Protein. Backed by our 100% Guarantee. ... Recombinant Human MAF1 His Protein Summary. Description. A ... Recombinant human MAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional ... Be the first to review our Recombinant Human MAF1 His Protein and receive a gift card or discount.. ... SDS-Page: Recombinant Human MAF1 Protein [NBP1-72440] - 15% SDS-PAGE (3ug) ...
Figure 1 - Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins - Volume 25, Number 1-January 2019 -...
Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins Alexandra Rockstroh, Luisa Barzon, Widuranga ... The antigens in this ELISA were Equad proteins (i.e., envelope protein from each DENV serotype with 4 amino acid changes T76R, ... Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins. ...
Preparation of Antibodies against Soluble Recombinant Dengue E Proteins Fused with Glutathione's Transferase.
Recombinant Proteins IL-2
Protein Expression | Recombinant Protein & rAb Services - GenScript
GenScript recombinant protein and rAb services provide high quality recombinant proteins and rAbs for a variety of downstream ... The Recombinant Protein Expression and Cell Line Development Service Handbook The Recombinant Protein Expression and Cell Line ... Antigen protein, Protein standards, Functional proteins Proteins with glycosylation, Assay standards, Secreted form Functional ... Recombinant Protein Expression Service Protein expression is almost universally used by research groups for a variety of ...
MBS1000074 | Recombinant 50S ribosomal protein L4 (rplD)
Recombinant 50S ribosomal protein L4 (rplD). Cat# MBS1000074. Supplier: MyBiosource. Available at Gentaur Genprice in 5 to 7 ... Recombinant 50S ribosomal protein L4 (rplD) MyBiosource Recombinant Proteins MBS1000074 , Recombinant 50S ribosomal protein L4 ... MyBiosource Recombinant Proteins. MBS1000074 , Recombinant 50S ribosomal protein L4 (rplD). Rating Required Select Rating. 1 ... Recombinant 50S ribosomal protein L4 (rplD) , MBS1058598 , MyBiosourceProduct Short Name: [50S ribosomal protein L4 (rplD)] ...
Complex coacervates based on recombinant mussel adhesive proteins: their characterization and applications - Soft Matter (RSC...
Complex coacervates based on recombinant mussel adhesive proteins: their characterization and applications H. J. Kim, B. Yang, ... Complex coacervates based on recombinant mussel adhesive proteins: their characterization and applications ... effective underwater adhesive have been made using complex coacervates that are based on recombinant mussel adhesive proteins ( ... Here, this review provides an overview of recombinant MAP-based complex coacervations, with an emphasis on their ...
Human Pleiotrophin (PTN) Protein (GST tag), Recombinant | ABIN1316795
Protein (GST tag). Spezies: Human. Quelle: Wheat germ. Jetzt Produkt ABIN1316795 bestellen. ... Protein-Typ Recombinant Proteineigenschaft AA 1-168 Spezies Alle Spezies für Pleiotrophin Proteine * Human 8 ... Pleiotrophin (PTN) protein PTN Spezies: Rind (Kuh) Wirt: Escherichia coli (E. coli) Recombinant , 95 % ELISA, IP, SDS, WB ... Pleiotrophin Protein (PTN) (AA 1-168) (GST tag). PTN Spezies: Human Wirt: Wheat germ Recombinant AP, AA, ELISA, WB ...
BTLA Recombinant Protein - Cat. No. 91-881 | ProSci
BTLA Recombinant protein (Cat. No. 91-881) has been validated for use in biological assays. ... Dissolve the lyophilized protein in ddH2O.. *STORAGE CONDITIONS: Lyophilized protein should be stored at -20°C, though stable ... APPLICATION NOTE: This recombinant protein can be used for biological assays. For research use only. ... Reconstituted protein solution can be stored at 4-7°C for 2-7 days.. Aliquots of reconstituted samples are stable at -20°C for ...
Immunogenicity and safety of a recombinant fusion protein vaccine (V-01) against coronavirus disease 2019 in healthy adults: a...
Recombinant Echo Virus Proteins - Jena Bioscience
Natural Proteins | Recombinant Proteins | Adooq Bioscience
Recombinant Rat Ccl19 protein, His-tagged Ccl19-723R - Creative BioMart
Recombinant Rat Ccl19 protein, His-tagged can be used for research. ... Purified Recombinant Rat Ccl19 protein, His-tagged from Creative Biomart. ... Home / Products / Recombinant Proteins / Recombinant Rat Ccl19 protein, His-tagged Recombinant Rat Ccl19 protein, His-tagged ... Ccl19-723R Optional Service: Optional requirements on this protein Product Overview :. Recombinant Rat Ccl19 aa. (Asp32~Lys103 ...
Recombinant Human Noggin protein (NOG) (Active) | CSB-AP003011HU | Cusabio
Recombinant Human Noggin protein (NOG) (Active) from Cusabio. Cat Number: CSB-AP003011HU. USA, UK & Europe Distribution. ... Recombinant Human Noggin protein (NOG) (Active) , CSB-AP003011HU Cusabio Active Proteins Recombinant Human Noggin protein (NOG ... Recombinant Human Noggin protein (NOG) (Active) , CSB-AP003011HU , Cusabio. Protein Description: Full Length of Mature Protein ... Recombinant Human Protein disulfide-isomerase protein (P4HB) (Active) , CSB-AP000091HU Cusabio Active Proteins ...
Recombinant Human Active SYK Protein, CF 4594-KS-010: R&D Systems
Human SYK protein (4594-KS) is manufactured by R&D Systems. Reproducible results in bioactivity assays. Learn More... ... as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, ... Reviews for Recombinant Human Active SYK Protein, CF. There are currently no reviews for this product. Be the first to review ... Have you used Recombinant Human Active SYK Protein, CF?. Submit a review and receive an Amazon gift card.. $25/€18/£15/$25CAN/¥ ...
CD2 Recombinant Protein
CD2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea. ... CD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 209 amino acids (25-209 a.a ... For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. ...
Mouse Aldehyde Oxidase 3 (AOX3) Protein (His tag), Recombinant | ABIN3131204
Protein (His tag). Species: Mouse. Source: Insect Cells. Order product ABIN3131204. ... Protein Type Recombinant Protein Characteristics AA 1-1335 Origin Mouse Source Insect Cells Purification tag / Conjugate This ... Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the ... The concentration of our recombinant proteins is measured using the absorbance at 280nm. The proteins absorbance will be ...
PeptidesVaccineGeneEscherichiaBaculovirusAntibodiesPrepared by recombinant DNA technoAntigenPurificationAssayQuality of recombinantEffect of recombinantAdministration of recombinantSubunitSequenceStabilityReagentsYeastCharacterizationFusion proteinSpecific recombinantCellsSpikeEnvelope ProteinsMembraneAntigensCoagulationConcentrationStableSpecificityExpressionAlfa-2aMammalianTransfectionBioactive proteinsAminoInhibitorAffinityCitationsHeterologousVaccinesDescriptionAdjuvantMolecularProductionVectorsGlycerol
- Peptides and proteins are guaranteed for 3 months from date of receipt. (novusbio.com)
- A previous phase I trial indicated that the recombinant COVID-19 vaccine (V-01), which contains a fusion protein (IFN-PADRE-RBD-Fc dimer) as its antigen, is safe and well tolerated, capable of inducing rapid and robust immune responses, and warranted further testing in additional clinical trials. (nih.gov)
- One of these proved to be a fatty acid binding protein (FABP) termed Sm14, which subsequently became the basis of the experimental vaccine herein discussed. (frontiersin.org)
- These results demonstrate that recombinant DEV expressing the TE and pre-membrane proteins is protective and can serve as a potential candidate vaccine to prevent DTMUV infection in ducks. (pasteur.fr)
- Peroxiredoxin is an antigenic protein and vaccine candidate antigen of T. gondii that has not yet been exploited for diagnostic application. (biomedcentral.com)
- Huvet Bio Inc. is developing a COVID-19 vaccine being using a sub-unit method with a high safety profile that uses proteins instead of viruses or bacteria. (who.int)
- Cellular and humoral immunity to Ebola Zaire glycoprotein and viral vector proteins following immunization with recombinant vesicular stomatitis virus-based Ebola vaccine (rVSVΔG-ZEBOV-GP). (bvsalud.org)
- While effective at preventing Zaire ebolavirus (ZEBOV) disease , cellular immunity to ZEBOV and vector-directed immunity elicited by the recombinant vesicular stomatitis virus expressing ZEBOV glycoprotein (rVSVΔG-ZEBOV-GP) vaccine remain poorly understood. (bvsalud.org)
- and four, protein subunit vaccine wherein the recombinant proteins of SARS-COV-2 along with an adjuvant (booster) is given as a vaccine. (moneycontrol.com)
- The ChAdOx1-S [recombinant] vaccine uses a DNA adenovirus vector to elicit antibodies to the SARS-CoV-2 spike protein. (who.int)
- QuantiLum Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis). (fishersci.com)
- P25 gene knockout silkworms may become an efficient bioreactor for the production of exogenous proteins and a promising tool for producing various protein-containing silk biomaterials. (semanticscholar.org)
- CusabioProtein Description: Full Length of Mature ProteinAlternative Name (s) : Chemokine-like protein TAFA-2,Gene. (joplink.net)
- The protein derived from the cloned gene exhibited significant homologies with a family of related polypeptides, which bind hydrophobic ligands, and purified recombinant protein exhibited an affinity to fatty acids. (frontiersin.org)
- In addition, the gene encoding human BDNF and processes for obtaining the same are disclosed.A previously-unreported member of the NGF/BDNF family of neurotrophic proteins, NGF-3, has been identified and a portion of the gene encoding for the NGF-3 has been described. (justia.com)
- As an alternative, it would be desirable to isolate the human gene for a neurotrophic factor and use that gene as the basis for establishing a recombinant expression system to produce potentially unlimited amounts of the human protein. (justia.com)
- IMSEAR at SEARO: Development of immunodetection system for botulinum neurotoxin type B using synthetic gene based recombinant protein. (who.int)
- A major focus here is on the development of new aptamer-based biosensors for the monitoring of cell culture processes as well as the design, production and integration of a new lab-on-a-chip system that enables continuous, transient gene transfer into host cells for the flexible production of recombinant proteins. (uni-augsburg.de)
- This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. (prosci-inc.com)
- The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. (origene.com)
- Within the last two decades, VPM has successfully managed and consulted the development of promising biopharmaceutical candidates, ranging from small molecules, recombinant proteins up to gene-modified live vaccines and ATMPs. (yourerie.com)
- COVID-19 can be diagnosed by detection of RNA gene targets (e.g. spike protein (S), an envelope protein (E), nucleocapsid protein (N), RNA-dependent RNA polymerase enzyme, and ORF1 gene) (4-6) either by nucleic acid amplification testing or detection of virus-specific proteins by antigen testing (7,8). (who.int)
- Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. (gentaur.com)
- The microorganism Escherichia coli is usually used for recombinant protein manufacturing. (stevediggle.net)
- Insect cells are co-transfected with wild type Autographa californica nuclear polyhedrosis virus DNA (AcNPV) and baculovirus transfer plasmids and isolates recombinant baculoviruses that arise by homologous recombination. (bcm.edu)
- Recombinant baculovirus stocks from the transfection or co-transfection are plaque purified by an agarose overlay assay. (bcm.edu)
- We titer and amplify chosen positive recombinant baculovirus isolates. (bcm.edu)
- We express proteins of interest(POIs) in insect cells using recombinant baculovirus. (bcm.edu)
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
- While high protein production could be achieved in the baculovirus/silkworm expression system, unintentional protein modification might occur, and therefore protein expression in the transgenic silkworms expression system is preferable from the point-of-view of N-glycosylation of the recombinant protein and evasion of unexpected attack by a protease in B. mori. (semanticscholar.org)
- THBD Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 500 amino acids (22-515a.a) and having a molecular mass of 52.6kDa. (prospecbio.com)
- Baculovirus/Insect expression system allows for expression of high quality glycosylated proteins that cannot be expressed using E.coli or yeast. (genscript.com)
- Preparation of Antibodies against Soluble Recombinant Dengue E Proteins Fused with Glutathione's Transferase. (who.int)
- Twenty five years later, recombinant CHO cell culture has emerged as one of the dominant methods for production of recombinant proteins, especially high-dose therapeutic antibodies. (unl.pt)
- He led the development of the first licensed, high-density, fed-batch cell culture process, a breakthrough platform technology now used by Genentech and throughout the industry for production of high-dose therapeutic antibodies and other proteins. (unl.pt)
- Animal study demonstrated that both recombinant viruses induced measurable anti-DTMUV neutralizing antibodies in ducks. (pasteur.fr)
- In this study, recombinant T. gondii peroxiredoxin protein (rTgPrx) was prepared and used in dot-immunogold-silver staining (Dot-IGSS) to detect IgG antibodies in serum from mice and pregnant women. (biomedcentral.com)
- IgM and IgG antibodies , IgG -producing memory B cells (MBCs), and T cell reactivity to ZEBOV glycoprotein (ZEBOV-GP), vesicular stomatitis virus - Indiana strain (VSV-I) matrix (M) protein , and VSV-I nucleoprotein (NP) were measured using ELISA , ELISpot , and flow cytometry , respectively. (bvsalud.org)
- The cassettes produce scalable results and can purify recombinant proteins or monoclonal antibodies. (pharmtech.com)
- Plot of all individual optical density (OD) ratios obtained from recombinant ELISA testing of human serum samples for Middle East respiratory syndrome coronavirus (MERS-CoV) antibodies, Africa, 2013-2014. (cdc.gov)
- CALIXAR's approach allows to preserve the original structure and function of membrane proteins (GPCRs, Ion Channels, Transporters, Receptors, Anchors and Viral Proteins) providing solutions for pharmaceutical industries, biotechnology companies and academic teams to develop conformational antibodies, formulate new vaccines, carry out Structure Based Drug Discovery and/or HTS assays. (calixar.com)
- Within the past 2 years, investigational peptide- and recombinant protein-based serologic assays that can more easily differentiate the antibodies to HTLV-I and HTLV-II have been developed (8,9). (cdc.gov)
Prepared by recombinant DNA techno1
- Proteins prepared by recombinant DNA technology. (bvsalud.org)
- IgM endpoint titers of DENV-1, DENV-2, and DENV-4 PCR-positive and NS1 antigen-positive serum samples from residents of dengue endemic countries Vietnam, Sri Lanka, Venezuela, and Brazil, 2013-2018, on DENV 1-4 Equad proteins. (cdc.gov)
- Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). (novusbio.com)
- For proteins that express insolubly, we can provide purification under denaturing condition (e.g. in high concentrations of urea). (bcm.edu)
- In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antibodies-online.com)
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antibodies-online.com)
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antibodies-online.com)
- Expression, purification, and characterization of recombinant humanpar" by YAĞMUR ÜNVER, ESABİ BAŞARAN KURBANOĞLU et al. (tubitak.gov.tr)
- Reduced glutathione is covalently linked Agarose 4B Microbeads for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. (agscientific.com)
- This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. (agscientific.com)
- After selection of a specific recombinant viral isolate, a passage #1 seed stock is expanded to a 500ml volume and the passage #2 viral stock is titered by an agarose overlay plaque assay to determine the virus concentration in pfu/ml. (bcm.edu)
- Human IgG antibody Laboratories manufactures the dc biorad protein assay lowrys reagents distributed by Genprice. (pcr-pcr.com)
- The Dc Biorad Protein Assay Lowrys reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (pcr-pcr.com)
- Description: A sandwich quantitative ELISA assay kit for detection of Mouse Tubulin Polymerization Promoting Protein (TPPP) in samples from tissue homogenates or other biological fluids. (1elisakits.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tubulin Polymerization Promoting Protein (TPPP) in tissue homogenates, cell lysates and other biological fluids. (1elisakits.com)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tubulin Polymerization Promoting Protein (TPPP) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (1elisakits.com)
- Lateral flow assay and ELISA techniques gave consistent results for IgG/IgM antibody measurements towards spike and nucleocapsid proteins, suggesting that both methods can be used to detect COVID-19 where access to molecular test kits is difficult. (who.int)
Quality of recombinant1
- Optimal operating conditions for both process and genetic variables are determined for producing improved quantity and quality of recombinant protein. (tufts.edu)
Effect of recombinant1
- OBJECTIVES: To explore the effect of recombinant, human GH on follicular development and oocyte retrieval after gonadotropin stimulation with the addition of GH or placebo to a standard IVF treatment regimen. (arctichealth.org)
Administration of recombinant2
- There is proof to point that in rodent fashions of CKD, administration of recombinant BMP1-Three will increase renal. (eb2009.org)
- The safest approach to modulate the immune or inflammatory pathways and cellular mechanisms is to either enhance or prevent these natural responses by administration of recombinant proteins of immune modulators produced also by the cells in the body. (neuroinflammation.eu)
- EUROIMMUN AG, Lübeck, Germany), which is based on the recombinant MERS-CoV spike protein subunit 1 and specifically detects IgG. (cdc.gov)
- For a protein with mammalian origin, the activity can be related not only to the primary structure (sequence), but also to the conformation and modifications, while other systems, especially when distantly related, cannot accomplish all the modifications found naturally in mammalian cells. (genscript.com)
- A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (G476S) -His Recombinant Protein (YP_009724390.1) (Arg319-Phe541 (G476S) ) was expressed with a polyhistidine tag at the C-terminus. (proteomecommons.org)
- The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (joplink.net)
- Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
- The development of recombinant allergens provides reagents that should improve the diagnostic accuracy of tests for latex allergy. (cdc.gov)
- Cytoskeleton offers several reagents for live-cell research including fluorescent proteins, cell permeable protein activators and inhibitors, as well as our recent addition of live cell imaging probes. (cytoskeleton.com)
- The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
- The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
- A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)
- Here, this review provides an overview of recombinant MAP-based complex coacervations, with an emphasis on their characterization and the uses of such materials for applications in the fields of biomedicine and tissue engineering. (rsc.org)
- cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein. (nih.gov)
- Introduction: A phase 3b extension study evaluated the long-term safety and efficacy of a recombinant fusion protein-linking coagulation factor IX (FIX) with albumin (rIX-FP) for the routine prophylaxis and on-demand treatment of bleeding in pediatric hemophilia B patients. (tau.ac.il)
- However, PLD1 precipitated from cell lysates with immobilized glutathione S-transferase-RalA fusion protein is active. (embl.de)
- In the past a series of specific recombinant protein headings were created to distinguish several pharmaceutically-significant protein classes. (bvsalud.org)
- For example, Rosetta™ cells are used for protein constructs containing a high number of rare E. coli codons, and Origami™ cells are used to encourage proper disulfide bond formation. (bcm.edu)
- Individual recombinant viral isolates are plucked from agarose and used to infect Sf9 insect cells. (bcm.edu)
- The infected Sf9 cells (for intracellular proteins) or culture supernatant (for secreted proteins) are provided to the investigator for expression screening and identification of the isolate with the highest level of expression. (bcm.edu)
- Crystallography grade MDH Spezies: CHO cells Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. (antikoerper-online.de)
- Recombinant proteins are primarily produced from cultures of mammalian, insect, and bacteria cells. (hindawi.com)
- In this paper, we review the recent progress in the methodology of agroinfiltration, a solution to overcome the challenge of transgene delivery into plant cells for large-scale manufacturing of recombinant proteins. (hindawi.com)
- MAF1 that is similar to Maf1, a Saccharomyces cerevisiae protein is a protein highly conserved in eukaryotic cells and is localized to the nucleus. (novusbio.com)
- Mammalian cells are more complex than bacterial cells and have the most sophisticated protein production capabilities including mechanisms to assist protein folding into the proper conformation like chaperones and modifications (glycosylation, methylation, phosphorylation etc. (genscript.com)
- GenScript employs genetically modified CHO and HEK293 cells together with elaborately designed expression vectors to give high yield and quality protein production, which has been proven by hundreds of successful projects. (genscript.com)
- Mouse Aox3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antibodies-online.com)
- They are raised in HEK293, CHO, and insect cells to offer you bioactive proteins with the optimal glycosylation profiles. (selectscience.net)
- Z102015An IGF-binding protein, IBP-5 is produced by fibroblasts, myoblasts, osteoblasts and vascular smooth muscle cells. (topsan.org)
- The protein was produced from HEK293T cells transfected with SCAMP2 cDNA clone. (bosterbio.com)
- Neurotrophic factors are natural proteins, found in the nervous system or in non-nerve tissues innervated by the nervous system, whose function is to promote the survival and maintain the phenotypic differentiation of nerve and/or glial cells (Varon and Bunge 1978 Ann. (justia.com)
- The research group uses 3D-printed microfluidic systems for the flexible production of recombinant proteins by enhanced transient transfection of mammalian cells. (uni-augsburg.de)
- The recombinant cells were grown in a shaking flask containing production medium. (tubitak.gov.tr)
- Antibody 4B4-1 interferes with binding of Biotinylated Recombinant CD137-huIg (502-030) to CD137L on Raji cells, but does not block binding of Recombinant CD137L-muCD8 (503-020) to CD137 on surface of stimulated CEM cells. (ancell.com)
- It is thus obvious that additional proteins produced by the human cells in these diseases displaying a potential benefit for CNS diseases need to be identified. (neuroinflammation.eu)
- One of the proteins, SARS-CoV-2 Spike Protein (S Protein), is a glycoprotein that mediates membrane fusion and viral entry, and as such is a crucial COVID-19 target. (selectscience.net)
- Find your one-stop-supplier for coronavirus proteins - including full-length Spike Proteins, Spike Subunits and Spike RBD. (selectscience.net)
- Recombinant Human ACE2 Protein (mFc Tag) (Active) (Cat#PKSR030509) at 2 μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, His Tag) (G476S) (Active) (Cat#PKSR030533) , the EC50 of PKSR030533 is 25-60 ng/mL. (proteomecommons.org)
- The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. (proteomecommons.org)
- The coronavirus genome encodes a spike protein, an envelope protein, a membrane protein, and a nucleoprotein. (creative-biolabs.com)
- Among them, spike protein is the most important surface membrane protein of coronavirus. (creative-biolabs.com)
- Passive immunity to porcine epidemic diarrhea virus following immunization of pregnant gilts with a recombinant orf virus vector expressing the spike protein. (harvard.edu)
- We report four nanobodies (C5, H3, C1, F2) engineered as homotrimers with pmolar affinity for the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. (bvsalud.org)
- Cryo Electron Microscopy shows C5 binding results in an all down arrangement of the Spike protein. (bvsalud.org)
- Equad proteins are the envelope proteins from DENV with 4 amino acid changes (T76R, Q77E, W101R, and L107R). (cdc.gov)
- 1. Rockstroh A, Barzon L, Pacenti M, Palù G, Niedrig M, Ulbert S. Recombinant envelope-proteins with mutations in the conserved fusion loop allow specific serological diagnosis of dengue-infections. (cdc.gov)
- B- and T-Lymphocyte Attenuator (BTLA) is a single-pass type I membrane protein containing 1 Ig-like V-type (immunoglobulin-like) domain. (prosci-inc.com)
- S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. (proteomecommons.org)
- One virus, rDEV-TE, expresses the truncated form of the envelope glycoprotein (TE) of duck tembusu virus (DTMUV), and the other virus, rDEV-PrM/TE, expresses both the TE and pre-membrane proteins (PrM). (pasteur.fr)
- This recombinant protein (r-Antidote, PRT064445) is catalytically inactive and lacks the membrane-binding γ-carboxyglutamic acid domain of native fXa but retains the ability of native fXa to bind direct fXa inhibitors as well as low molecular weight heparin-activated antithrombin III (ATIII). (nih.gov)
- Pall Corp. (East Hills, NY, www.pall.com ) recently unveiled its 'Omega T' series membrane cassettes for biopharmaceutical processing applications involving high protein concentrations. (pharmtech.com)
- The screen also supports high mass transfer of proteins and minimizes the gel layer that normally builds up on the membrane. (pharmtech.com)
- Starting from native material or recombinant systems, we succeed with all types of membrane proteins: GPCRs, Ions Channels, Transporters, Receptors and Viral Proteins. (calixar.com)
- CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). (ancell.com)
- Arf proteins: the membrane traffic police? (embl.de)
- sensitivity to membrane phospholipids--each of these activities has been attributed to Arf proteins. (embl.de)
- The antigens in this ELISA were Equad proteins (i.e., envelope protein from each DENV serotype with 4 amino acid changes T76R, Q77E, W101R, and L107R). (cdc.gov)
- Recombinant human APC inhibits factors Va and VIIIa of the coagulation cascade. (medscape.com)
- NRL is harvested from Hevea brasiliensis trees and ammoniated to prevent coagulation resulting in the hydrolysis of the latex proteins. (cdc.gov)
- Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20? (joplink.net)
- The concentration of our recombinant proteins is measured using the absorbance at 280nm. (antibodies-online.com)
- The concentration of the protein is calculated using its specific absorption coefficient. (antibodies-online.com)
- The recombinant protein expression was optimized using various concentration of isopropylthiogalactoside (IPTG) induction, further the expression was confirmed by Western blot analysis using anti-His antibody. (who.int)
- Results: The recombinant protein was expressed at maximum yield at 4.3 h of post-induction with 0.5 mM IPTG concentration. (who.int)
- Abstract: In this work, the design and execution of a mechanistic metabolic model is presented that is capable of simulating extracellular metabolite concentration profiles, particularly cell density and antibody titer, throughout the course of a recombinant protein producing CHO fed-batch culture. (tufts.edu)
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. (prosci-inc.com)
- Further, this method can be applied under broad buffer, pH and temperature conditions, conferring the potential to apply to other thermal stable proteins. (researchsquare.com)
- Structures of Orf Virus Chemokine Binding Protein in Complex with Host Chemokines Reveal Clues to Broad Binding Specificity. (harvard.edu)
- TRX, MBP, EGFP) in order to maximize the expression level of the target protein in E. coli. (bcm.edu)
- Depending on the nature of the heterologous target protein, we provide and utilize a number of different E. coli host strains to improve expression levels and to maximize the odds of proper folding. (bcm.edu)
- We construct recombinant baculoviruses with expression DNA vectors/bacmids provided by the investigator. (bcm.edu)
- These new advancements in agroinfiltration greatly enhance the robustness and scalability of transgene delivery in plants, facilitating the adoption of plant transient expression systems for manufacturing recombinant proteins with a broad range of applications. (hindawi.com)
- By employing genome editing technology to reduce silk protein expression levels, a silkworm bioreactor expression system could be developed as a highly successful system for producing various valuable heterologous proteins, potentially broadening the applications of the silkworm. (semanticscholar.org)
- A novel method to enhance recombinant protein expression in MSGBioreactor, but also a strategy to optimize other bioreactor systems is proposed. (semanticscholar.org)
- Protein expression is almost universally used by research groups for a variety of purposes. (genscript.com)
- As a leading provider of a comprehensive suite of recombinant protein and antibody services across different expression systems, GenScript seeks to partner with scientists worldwide to accelerate scientific discovery. (genscript.com)
- The features of a target protein inform which expression system is best suited for the expression of that protein, and it is important to identify the correct system for protein expression based on these criteria. (genscript.com)
- E.coli is one of the most commonly used protein expression systems and protein expression is usually induced using a DNA plasmid expression vector. (genscript.com)
- In the case that PTMs are not important in the target protein, E.coli can still be a good choice for even eukaryotic protein expression. (genscript.com)
- The main purpose of the present study was to perform the expression in Pichia pastoris X-33 of the human paraoxonase 1 (hPON1) enzyme, which is a mammalian serum protein. (tubitak.gov.tr)
- Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. (atlasantibodies.com)
- Note that defined pharmaceutical preparations of individual recombinant proteins, e.g., interferon alfa-2a, will be maintained in the supplemental concept record. (bvsalud.org)
- A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
- They also have superior machinery for folding mammalian proteins and therefore give the best chance of obtaining soluble protein when a protein of mammalian origin is expressed. (genscript.com)
- This is a reason why many researchers believe the activity of mammalian proteins is best when expressed in a mammalian cell. (genscript.com)
- Recombinant baculoviruses can also be generated by transfection of bacmid DNA using the Bac-to-Bac system. (bcm.edu)
- So far, recombinantly expressed spidroins often form insoluble inclusion bodies (IBs), which will often be dissolved under extremely harsh conditions in a traditional manner, e.g. either 8 mol/L urea or 6 mol/L guanidine hydrochloride, highly risking to poor recovery of bioactive proteins as well as unexpected precipitations during dialysis process. (researchsquare.com)
- CD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 209 amino acids (25-209 a.a) and having a molecular mass of 23.8kDa. (neuromics.com)
- Equad, envelope protein with 4 amino acid changes. (cdc.gov)
- The mutations consist of amino acid substitutions at position 505 in familial cases or 515 in sporadic cases These result in constitutive activation of the thrombopoietin receptor protein. (medscape.com)
- Human recombinant protein fragment corresponding to amino acids 1-266 of human PARN (NP_002573) produced in E.coli. (origene.com)
- Recombinant Murine IFN- λ2 is a 19.8 kDa protein containing 175 amino acid residues. (peprotech.com)
- Most of these mutations change one protein building block (amino acid) in the pyridoxine 5'-phosphate oxidase enzyme, impairing its normal function. (medlineplus.gov)
- The ARFs are a family of 21,000 M(r) proteins with biological roles in constitutive secretion and activation of phospholipase D. The structure of ARF-1 complexed to GDP determined from two crystal forms reveals a topology that is similar to that of the protein p21 ras with two differences: an additional amino-terminal helix and an extra beta-strand. (embl.de)
- We hypothesize that this lack of detectable signs of disease during infection with BA.4 was due to a small (nine nucleotide) deletion (∆686-694) in the viral genome (ORF1ab) responsible for production of non-structural protein 1 which resulted in the loss of three amino acids (aa 141-143). (bvsalud.org)
- Free shipping on inhibitor and protein orders over $500. (adooq.com)
- Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. (joplink.net)
- CusabioAlternative Name(s): Ectodermal BMP inhibitor Short name: Ectodin Sclerostin-like protein Uterine. (joplink.net)
- The effect of r-Antidote was mediated by reducing plasma anti-fXa activity and the non-protein bound fraction of the fXa inhibitor in plasma. (nih.gov)
- If there are concerns about affinity tags or fusion partners interfering with the structure and function of the target protein, we can provide reliable methods for removing affinity tags. (bcm.edu)
- Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (bosterbio.com)
- Recombinant protein was purified under denatured condition using Ni-NTA affinity chromatography. (who.int)
- The recombinant protein was purified using Ni-NTA affinity chromatography up to the homogeneity level. (who.int)
- Citations will be maintained to the appropriate protein classes and the general heading 'Recombinant Proteins. (bvsalud.org)
- The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. (proteomecommons.org)
- Of the 5 meningococcal vaccines available in the United States: 3 are conjugate vaccines and 2 are recombinant protein vaccines. (cdc.gov)
- Both recombinant protein vaccines are monovalent vaccines. (cdc.gov)
- The generic group of ChAdOx1-S [recombinant] vaccines includes AstraZeneca/AZD1222 and SII/Covishield vaccines. (who.int)
- WHO SAGE interim recommendations on the AstraZeneca COVID-19 vaccines refer to a generic group of ChAdOx1-S [recombinant] vaccines against COVID-19. (who.int)
- Description: Host cell factor 2(HCFC2) is a cytoplasmic protein. (gentaurtop.com)
- Antibody was generated against the recombinant protein using alum adjuvant in BALB/c mice and tested for cross reactivity with other serotypes of C. botulinum as well as closely related clostridia. (who.int)
- Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
- In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. (antibodies-online.com)
- The predicted molecular weight of Recombinant Mouse gAcrp30 is Mr 16.7 kDa. (leinco.com)
- POLR3H Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 227a.a. (1-204 a.a.) and having a molecular weight of 25.3kDa (Molecular size on SDS-PAGE will appear higher).POLR3H is fused to a 23a.a. (betalifesci.com)
- SDS-PAGE and Western blot analysis illustrated that the molecular mass of extracellular hPON1 enzyme produced by the recombinant P. pastoris strain was 59.1 kDa. (tubitak.gov.tr)
- The bulk of the amplified 500ml virus stock is stored at 4° C for use in protein production. (bcm.edu)
- In recent years, the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein production, with advantages in versatility, speed, cost, scalability, and safety over the current production paradigms. (hindawi.com)
- The approval of the first plant-derived therapeutic enzyme for Gaucher's disease has demonstrated the promise of plant-based systems for recombinant protein (RP) production [ 1 ]. (hindawi.com)
- To utilize this capacity for mass production of useful proteins, transgenic silkworms were generated that synthesized recombinant proteins in the silk gland and secreted them into the silk cocoon. (semanticscholar.org)
- Made in Germany - from design to production - by highly experienced protein experts. (antibodies-online.com)
- At the time, many viewed recombinant cell culture as a production method of last resort, certainly not one suitable to make a high-dose therapeutic. (unl.pt)
- Octapharma AB (OAB) in Stockholm, Sweden, is the site for Nuwiq human recombinant factor VIII (FVIII), production. (bioprocessintl.com)
- This invention relates to processes for the production of recombinant members of the human NGF/BDNF family of neurotrophic proteins in biologically active forms. (justia.com)
- In addition, this invention discloses processes for identifying previously unreported members of this family of proteins and the subsequent production of these proteins. (justia.com)
- Furthermore, the group deals with biocompatibility studies to ensure an ideal environment for cell viability and recombinant protein production. (uni-augsburg.de)
- Time-course and dose-response studies have shown subcutaneous, intratracheal, or topical administrations of non-ammoniated latex proteins to induce IgE production. (cdc.gov)
- PLP is the active form of vitamin B6 and is necessary for many processes in the body, including protein metabolism and the production of chemicals that transmit signals in the brain (neurotransmitters). (medlineplus.gov)
- However, the advent of recombinant protein technology for the production of most pharmaceutical proteins has made this distinction obsolete. (bvsalud.org)
- The discussion of agroinfiltration vectors focuses on their applications for producing complex and heteromultimeric proteins and is updated with the development of bridge vectors. (hindawi.com)
- THBD protein solution (0.5mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 10% glycerol. (prospecbio.com)
- POLR3H protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT. (betalifesci.com)