Recombinant fusion proteins. Medical search

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Proteins prepared by recombinant DNA technology.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.

Semisynthetic conjugates of various toxic molecules, including RADIOACTIVE ISOTOPES and bacterial or plant toxins, with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; and ANTIGENS. The antitumor or antiviral immune substance carries the toxin to the tumor or infected cell where the toxin exerts its poisonous effect.

The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes.

Sites on an antigen that interact with specific antibodies.

Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.

Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.

DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.

Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.

Established cell cultures that have the potential to propagate indefinitely.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.

Antibodies produced by a single clone of cells.

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

Proteins found in any species of bacterium.

Proteins found in any species of protozoan.

The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.

The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.

Glycoproteins found on the membrane or surface of cells.

Substances elaborated by bacteria that have antigenic activity.

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Immunoglobulins produced in a response to BACTERIAL ANTIGENS.

Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.

The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.

The rate dynamics in chemical or physical systems.

The sum of the weight of all the atoms in a molecule.

The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.

Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.

The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology.

Operative immobilization or ankylosis of two or more vertebrae by fusion of the vertebral bodies with a short bone graft or often with diskectomy or laminectomy. (From Blauvelt & Nelson, A Manual of Orthopaedic Terminology, 5th ed, p236; Dorland, 28th ed)

Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.

Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.

Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.

Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.

Proteins that catalyze MEMBRANE FUSION.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.

Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

Periplasmic proteins that bind MALTOSE and maltodextrin. They take part in the maltose transport system of BACTERIA.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.

Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.

A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.

Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.

Transport proteins that carry specific substances in the blood or across cell membranes.

A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.

Proteins found in any species of virus.

The GENETIC RECOMBINATION of the parts of two or more GENES, including an ONCOGENE as at least one of the fusion partners. Such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS.

Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.

Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.

CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.

A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.

CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)

Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.

Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.

A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.

A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.

Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.

Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

The functional hereditary units of BACTERIA.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Deletion of sequences of nucleic acids from the genetic material of an individual.

A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

Immunoglobulins produced in response to VIRAL ANTIGENS.

The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.

Multinucleated masses produced by the fusion of many cells; often associated with viral infections. In AIDS, they are induced when the envelope glycoprotein of the HIV virus binds to the CD4 antigen of uninfected neighboring T4 cells. The resulting syncytium leads to cell death and thus may account for the cytopathic effect of the virus.

A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.

VEGF is required for growth and survival in neonatal mice. (1/41883)

We employed two independent approaches to inactivate the angiogenic protein VEGF in newborn mice: inducible, Cre-loxP- mediated gene targeting, or administration of mFlt(1-3)-IgG, a soluble VEGF receptor chimeric protein. Partial inhibition of VEGF achieved by inducible gene targeting resulted in increased mortality, stunted body growth and impaired organ development, most notably of the liver. Administration of mFlt(1-3)-IgG, which achieves a higher degree of VEGF inhibition, resulted in nearly complete growth arrest and lethality. Ultrastructural analysis documented alterations in endothelial and other cell types. Histological and biochemical changes consistent with liver and renal failure were observed. Endothelial cells isolated from the liver of mFlt(1-3)-IgG-treated neonates demonstrated an increased apoptotic index, indicating that VEGF is required not only for proliferation but also for survival of endothelial cells. However, such treatment resulted in less significant alterations as the animal matured, and the dependence on VEGF was eventually lost some time after the fourth postnatal week. Administration of mFlt(1-3)-IgG to juvenile mice failed to induce apoptosis in liver endothelial cells. Thus, VEGF is essential for growth and survival in early postnatal life. However, in the fully developed animal, VEGF is likely to be involved primarily in active angiogenesis processes such as corpus luteum development. (+info)

Cell polarization: chemotaxis gets CRACKing. (2/41883)

An early stage in the establishment of cell polarity during chemotaxis of Dictyostelium dicoideum has been identified by a recent study; the new results also show that the development of cell polarity does not rely upon cytoskeletal rearrangement, and may use a spatial sensing mechanism. (+info)

Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization. (3/41883)

The Drosophila Inscuteable protein acts as a key regulator of asymmetric cell division during the development of the nervous system [1] [2]. In neuroblasts, Inscuteable localizes into an apical cortical crescent during late interphase and most of mitosis. During mitosis, Inscuteable is required for the correct apical-basal orientation of the mitotic spindle and for the asymmetric segregation of the proteins Numb [3] [4] [5], Prospero [5] [6] [7] and Miranda [8] [9] into the basal daughter cell. When Inscuteable is ectopically expressed in epidermal cells, which normally orient their mitotic spindle parallel to the embryo surface, these cells reorient their mitotic spindle and divide perpendicularly to the surface [1]. Like the Inscuteable protein, the inscuteable RNA is asymmetrically localized [10]. We show here that inscuteable RNA localization is not required for Inscuteable protein localization. We found that a central 364 amino acid domain - the Inscuteable asymmetry domain - was necessary and sufficient for Inscuteable localization and function. Within this domain, a separate 100 amino acid region was required for asymmetric localization along the cortex, whereas a 158 amino acid region directed localization to the cell cortex. The same 158 amino acid fragment could localize asymmetrically when coexpressed with the full-length protein, however, and could bind to Inscuteable in vitro, suggesting that this domain may be involved in the self-association of Inscuteable in vivo. (+info)

Bcl-2 regulates amplification of caspase activation by cytochrome c. (4/41883)

Caspases, a family of specific proteases, have central roles in apoptosis [1]. Caspase activation in response to diverse apoptotic stimuli involves the relocalisation of cytochrome c from mitochondria to the cytoplasm where it stimulates the proteolytic processing of caspase precursors. Cytochrome c release is controlled by members of the Bcl-2 family of apoptosis regulators [2] [3]. The anti-apoptotic members Bcl-2 and Bcl-xL may also control caspase activation independently of cytochrome c relocalisation or may inhibit a positive feedback mechanism [4] [5] [6] [7]. Here, we investigate the role of Bcl-2 family proteins in the regulation of caspase activation using a model cell-free system. We found that Bcl-2 and Bcl-xL set a threshold in the amount of cytochrome c required to activate caspases, even in soluble extracts lacking mitochondria. Addition of dATP (which stimulates the procaspase-processing factor Apaf-1 [8] [9]) overcame inhibition of caspase activation by Bcl-2, but did not prevent the control of cytochrome c release from mitochondria by Bcl-2. Cytochrome c release was accelerated by active caspase-3 and this positive feedback was negatively regulated by Bcl-2. These results provide evidence for a mechanism to amplify caspase activation that is suppressed at several distinct steps by Bcl-2, even after cytochrome c is released from mitochondria. (+info)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (5/41883)

In COS cells, Ral GDP dissociation stimulator (RalGDS)-induced Ral activation was stimulated by RasG12V or a Rap1/Ras chimera in which the N-terminal region of Rap1 was ligated to the C-terminal region of Ras but not by Rap1G12V or a Ras/Rap1 chimera in which the N-terminal region of Ras was ligated to the C-terminal region of Rap1, although RalGDS interacted with these small GTP-binding proteins. When RasG12V, Ral and the Rap1/Ras chimera were individually expressed in NIH3T3 cells, they localized to the plasma membrane. Rap1Q63E and the Ras/Rap1 chimera were detected in the perinuclear region. When RalGDS was expressed alone, it was abundant in the cytoplasm. When coexpressed with RasG12V or the Rap1/Ras chimera, RalGDS was detected at the plasma membrane, whereas when coexpressed with Rap1Q63E or the Ras/Rap1 chimera, RalGDS was observed in the perinuclear region. RalGDS which was targeted to the plasma membrane by the addition of Ras farnesylation site (RalGDS-CAAX) activated Ral in the absence of RasG12V. Although RalGDS did not stimulate the dissociation of GDP from Ral in the absence of the GTP-bound form of Ras in a reconstitution assay using the liposomes, RalGDS-CAAX could stimulate it without Ras. RasG12V activated Raf-1 when they were coexpressed in Sf9 cells, whereas RasG12V did not affect the RalGDS activity. These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral, but that Rap1 does not activate Ral due to distinct subcellular localization. (+info)

B-MYB transactivates its own promoter through SP1-binding sites. (6/41883)

B-MYB is an ubiquitous protein required for mammalian cell growth. In this report we show that B-MYB transactivates its own promoter through a 120 bp segment proximal to the transcription start site. The B-MYB-responsive element does not contain myb-binding sites and gel-shift analysis shows that SP1, but not B-MYB, protein contained in SAOS2 cell extracts binds to the 120 bp B-myb promoter fragment. B-MYB-dependent transactivation is cooperatively increased in the presence of SP1, but not SP3 overexpression. When the SP1 elements of the B-myb promoter are transferred in front of a heterologous promoter, an increased response to B-MYB results. In contrast, c-MYB, the prototype member of the Myb family, is not able to activate the luciferase construct containing the SP1 elements. With the use of an SP1-GAL4 fusion protein, we have determined that the cooperative activation occurs through the domain A of SP1. These observations suggest that B-MYB functions as a coactivator of SP1, and that diverse combinations of myb and SP1 sites may dictate the responsiveness of myb-target genes to the various members of the myb family. (+info)

A single membrane-embedded negative charge is critical for recognizing positively charged drugs by the Escherichia coli multidrug resistance protein MdfA. (7/41883)

The nature of the broad substrate specificity phenomenon, as manifested by multidrug resistance proteins, is not yet understood. In the Escherichia coli multidrug transporter, MdfA, the hydrophobicity profile and PhoA fusion analysis have so far identified only one membrane-embedded charged amino acid residue (E26). In order to determine whether this negatively charged residue may play a role in multidrug recognition, we evaluated the expression and function of MdfA constructs mutated at this position. Replacing E26 with the positively charged residue lysine abolished the multidrug resistance activity against positively charged drugs, but retained chloramphenicol efflux and resistance. In contrast, when the negative charge was preserved in a mutant with aspartate instead of E26, chloramphenicol recognition and transport were drastically inhibited; however, the mutant exhibited almost wild-type multidrug resistance activity against lipophilic cations. These results suggest that although the negative charge at position 26 is not essential for active transport, it dictates the multidrug resistance character of MdfA. We show that such a negative charge is also found in other drug resistance transporters, and its possible significance regarding multidrug resistance is discussed. (+info)

Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers. (8/41883)

Gene expression in higher eukaryotes appears to be regulated by specific combinations of transcription factors binding to regulatory sequences. The Ets factor PU.1 and the IRF protein Pip (IRF-4) represent a pair of interacting transcription factors implicated in regulating B cell-specific gene expression. Pip is recruited to its binding site on DNA by phosphorylated PU.1. PU.1-Pip interaction is shown to be template directed and involves two distinct protein-protein interaction surfaces: (i) the ets and IRF DNA-binding domains; and (ii) the phosphorylated PEST region of PU.1 and a lysine-requiring putative alpha-helix in Pip. Thus, a coordinated set of protein-protein and protein-DNA contacts are essential for PU.1-Pip ternary complex assembly. To analyze the function of these factors in vivo, we engineered chimeric repressors containing the ets and IRF DNA-binding domains connected by a flexible POU domain linker. When stably expressed, the wild-type fused dimer strongly repressed the expression of a rearranged immunoglobulin lambda gene, thereby establishing the functional importance of PU.1-Pip complexes in B cell gene expression. Comparative analysis of the wild-type dimer with a series of mutant dimers distinguished a gene regulated by PU.1 and Pip from one regulated by PU.1 alone. This strategy should prove generally useful in analyzing the function of interacting transcription factors in vivo, and for identifying novel genes regulated by such complexes. (+info)

https://www.medicinenet.com › Medical Dictionary › G

A genetic translocation is a change in the number or arrangement of the chromosomes in a cell. It occurs when a portion of one chromosome breaks off and attaches to another chromosome. This can result in a gain or loss of genetic material, which can have significant effects on the individual.

Genetic Translocation | Definition & Facts | Britannica
https://www.britannica.com › science › Genetic-tr...

Genetic translocation, also called chromosomal translocation, a type of chromosomal aberration in which a portion of one chromosome breaks off and attaches to another chromosome. This can result in a gain or loss of genetic material. Genetic translocations are often found in cancer cells and may play a role in the development and progression of cancer.

Translocation, Genetic | health Encyclopedia - UPMC
https://www.upmc.com › health-library › gene...

A genetic translocation is a change in the number or arrangement of the chromosomes in a cell. It occurs when a portion of one chromosome breaks off and attaches to another chromosome. This can result in a gain or loss of genetic material, which can have significant effects on the individual.

Genetic Translocation | Genetics Home Reference - NIH
https://ghr.nlm.nih.gov › condition › ge...

A genetic translocation is a change in the number or arrangement of the chromosomes in a cell. It occurs when a portion of one chromosome breaks off and attaches to another chromosome. This can result in a gain or loss of genetic material, which can have significant effects on the individual.

In conclusion, Genetic Translocation is an abnormality in the number or arrangement of chromosomes in a cell. It occurs when a portion of one chromosome breaks off and attaches to another chromosome, resulting in a gain or loss of genetic material that can have significant effects on the individual.

"Coagulation Factor IX (Recombinant), Fc Fusion Protein". Drug Information Portal. U.S. National Library of Medicine. " ... The factor IX protein is composed of four protein domains: the Gla domain, two tandem copies of the EGF domain and a C-terminal ... Deficiency of this protein causes haemophilia B. It was discovered in 1952 after a young boy named Stephen Christmas was found ... A structure of the two EGF domains and the trypsin-like domain was determined for the pig protein. The structure of the Gla ...

https://en.wikipedia.org/wiki/Factor_IX

Characterization of cDNAs and recombinant fusion proteins". Eur. J. Biochem. 259 (3): 618-25. doi:10.1046/j.1432-1327.1999. ... Plasminogen-related protein B is a protein that in humans is encoded by the PLGLB2 gene. GRCh38: Ensembl release 89: ... v t e (Genes on human chromosome 2, All stub articles, Protein stubs). ...

https://en.wikipedia.org/wiki/PLGLB2

... is a recombinant human coagulation factor VIII, Fc fusion protein (rFVIIIFc). It is produced by recombinant ... "Eloctate (antihemophilic factor- recombinant, fc fusion protein kit". DailyMed. 4 August 2020. Retrieved 3 September 2020. " ... Recombinant proteins, All stub articles, Blood and blood forming organ drug stubs). ...

https://en.wikipedia.org/wiki/Efmoroctocog_alfa

Kreitman RJ, Pastan I (October 2011). "Antibody fusion proteins: anti-CD22 recombinant immunotoxin moxetumomab pasudotox". ... a condition in which fluid and proteins leak out of tiny blood vessels into surrounding tissues. Symptoms of capillary leak ...

https://en.wikipedia.org/wiki/Moxetumomab_pasudotox

... is a fusion protein produced by recombinant DNA. It fuses the TNF receptor to the constant end of the IgG1 antibody ... Finally, they expressed the linked DNA to produce a protein that links the protein for TNF receptor 2 to the protein for IgG1 ... The prototypic fusion protein was first synthesized and shown to be highly active and unusually stable as a modality for ... The fusion protein was developed by Bruce A. Beutler, an academic researcher then at the University of Texas Southwestern ...

https://en.wikipedia.org/wiki/Etanercept

1990). "Antigenicity of a recombinant Ro (SS-A) fusion protein". Arthritis Rheum. 33 (1): 102-6. doi:10.1002/art.1780330114. ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... 60 kDa SS-A/Ro ribonucleoprotein is a protein that in humans is encoded by the TROVE2 gene. GRCh38: Ensembl release 89: ... 2005). "RNA chaperone activity of protein components of human Ro RNPs". RNA. 11 (7): 1084-94. doi:10.1261/rna.7263905. PMC ...

https://en.wikipedia.org/wiki/TROVE2

November 2018). "Recombinant factor VIII Fc fusion protein drives regulatory macrophage polarization". Blood Advances. 2 (21): ... These cells can be distinguished based on the different expression levels of various surface proteins and the secretion of ...

https://en.wikipedia.org/wiki/Regulatory_macrophages

APO010, also known as mega-FasLigand is a recombinant fusion protein. This protein was derived from the pro-apoptotic human Fas ... APO200 is a therapeutic recombinant protein that is developed from the ectodysplasin A-1 (EDA1) gene. This product is used to ... A current target is Heat Shock Protein 90 (HSP90). HSP90 is key in protein folding and maturation. TopoTarget is currently ... pg 20 The recombinant protein product targets the ErbB2/HER2 receptor, which is overexpressed in 30% of breast cancer and is ...

https://en.wikipedia.org/wiki/TopoTarget

September 2020). "Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein". ... is developing specific peptide mimetic with the bioluminescent Gaussia luciferase fused as one protein that can bind ...

https://en.wikipedia.org/wiki/Zearalenone

... ged fusion proteins can be expressed using standard recombinant protein expression techniques. Furthermore, there are ... Once the fusion protein has been expressed, there is a wide range of potential areas of experimentation including enzymatic ... Protein tag SNAP tag SpyTag Fluorescent proteins Los GV, Encell LP, McDougall MG, Hartzell DD, Karassina N, Zimprich C, et al ... HaloTag is a self-labeling protein tag. It is a 297 residue protein (33 kDa) derived from a bacterial enzyme, designed to ...

https://en.wikipedia.org/wiki/HaloTag

Einhauer A, Jungbauer A (2001). "The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins". ... FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA ... FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. ... If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an ...

https://en.wikipedia.org/wiki/FLAG-tag

Einhauer, A.; Jungbauer, A. (2001). "The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins". ... Protein tags are peptide sequences genetically grafted onto a recombinant protein. Tags are attached to proteins for various ... This tag is used for protein purification of recombinant proteins and its fragments. It can be used in research labs and it is ... especially for recombinant proteins expressed in species such as E. coli, to assist in the proper folding in proteins and keep ...

https://en.wikipedia.org/wiki/Protein_tag

"Recombinant SARS-CoV-2 Fusion Protein Vaccine (V-01) Phase III (COVID-19)". ClinicalTrials.gov. 27 October 2021. NCT05096845. ... "Safety and Immunogenicity of Recombinant Protein RBD Fusion Dimer Vaccine Against the Virus That Cause COVID-19, Known as ... "Study of Recombinant Protein Vaccine with Adjuvant against COVID-19 in Adults 18 Years of Age and Older". pactr.samrc.ac.za. ... "Study of Recombinant Protein Vaccine With Adjuvant Against COVID-19 in Adults 18 Years of Age and Older (VAT00002)". ...

https://en.wikipedia.org/wiki/COVID-19_vaccine_clinical_research

Sometimes recombinant fusion proteins containing a toxin and a growth factor are also referred to as recombinant immunotoxins, ... A more specific name for this latter kind of protein is recombinant fusion toxin. They were originally produced by attaching ... The toxin is usually an AB toxin, a cytotoxic protein derived from a bacterial or plant protein, from which the natural binding ... An immunotoxin is an artificial protein consisting of a targeting portion linked to a toxin. When the protein binds to that ...

https://en.wikipedia.org/wiki/Immunotoxin

One of the main uses of this protein is for removing affinity tags from purified recombinant fusion proteins. The reason for ... "Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase". Anal. Biochem. 216 (2): 413- ... Kapust RB, Waugh DS (July 2000). "Controlled intracellular processing of fusion proteins by TEV protease". Protein Expr. Purif ... "Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro". Protein ...

https://en.wikipedia.org/wiki/TEV_protease

A recombinant fusion protein is a protein created through genetic engineering of a fusion gene. This typically involves ... Examples include: Gag-onc fusion protein Bcr-abl fusion protein Tpr-met fusion protein Antibodies are fusion proteins produced ... in contrast to genetic fusion prior to translation used in other recombinant technologies. Protein linkers aid fusion protein ... Recombinant fusion proteins are created artificially by recombinant DNA technology for use in biological research or ...

https://en.wikipedia.org/wiki/Fusion_protein

... is a recombinant fusion protein designed to inhibit B cells, thereby suppressing autoimmune disease. The designer ... The subcutaneously injected protein failed a phase II trial for multiple sclerosis. The trials of atacicept in people with MS ... a transmembrane receptor protein found predominantly on the surface of B cells. Like the monoclonal antibody belimumab, ... protein combines the binding site for two cytokines that regulate maturation, function, and survival of B cells - B-lymphocyte ...

https://en.wikipedia.org/wiki/Atacicept

This kind of vaccine can be realized by DNA recombinant fusion proteins expressed in E. coli. The protein MAGE-3 has already ... Clinical vaccination studies using full-length recombinant proteins have the advantage that this form of antigen potentially ... Two cohorts were analyzed: one receiving MAGE-3 protein alone, and one receiving MAGE-3 protein with adjuvant AS028. Of nine ... recombinant viral vaccines and recombinant bacterial vaccines. While most adoptive transfer experiments have been performed ...

https://en.wikipedia.org/wiki/Cancer_vaccine_targeting_CD4 _T_cells

NGR-hTNF is a recombinant protein derived from the fusion between peptide CNGRCG and human tumor necrosis factor alpha (TNFα). ...

https://en.wikipedia.org/wiki/NGR-hTNF_(antitumor_recombinant_protein)

... and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium". Applied and ... Ilk, N.; Egelseer, E.M.; Sleytr, U.B. (2011). "S-layer fusion proteins-construction principles and applications". Current ... S-layers are composed of single proteins that form a predictable lattice structure and have potential applications in ...

https://en.wikipedia.org/wiki/Sporosarcina_ureae

The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease). Fellers ...

https://en.wikipedia.org/wiki/Nuclear-inclusion-a_endopeptidase

... is a recombinant fusion protein consisting of a combination of CD4 and the fragment crystallizable region. It ... 2007). "Nonlinear pharmacokinetics of high-dose recombinant fusion protein CD4-IgG2 (PRO 542) observed in HIV-1-infected ... CD4 is a surface receptor for human immunodeficiency virus (HIV). The properties of the protein means that it has potential to ... Engineered proteins, All stub articles, Protein stubs). ...

https://en.wikipedia.org/wiki/CD4_immunoadhesin

... recombinant restriction-grade protease) cleavage site. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that ... Protein DHX8 is part of a protein complex called spliceosome, which is in charge of pre-mRNA splicing. The spliceosome has ... One example of a purification approach for DHX8 is via a protein tag called GST-His grafted onto DHX8 protein is used. The N- ... DEAH-box helicase 8, is a protein that in humans is encoded by the DHX8 gene. This protein is member of the DEAH box ...

https://en.wikipedia.org/wiki/DHX8

... a recombinant tumor necrosis factor receptor:Fc fusion protein, in patients with rheumatoid arthritis receiving methotrexate". ... The development of recombinant DNA technology in the 1970s shaped the modern understanding of what constitutes as biological ... Today, biological therapy most commonly refers to the use of proteins, such as monoclonal antibodies, to regulate the immune ... Certolizumab pegol is a recombinant antigen-binding fragment antibody that is attached to a 40kDa polyethylene glycol. The ...

https://en.wikipedia.org/wiki/Biological_therapy_for_inflammatory_bowel_disease

Zaltrap (Aflibercept), recombinant fusion protein, approved in metastatic colorectal cancer in combination with other agents in ... Zaltrap (Aflibercept), recombinant fusion protein, approved in metastatic colorectal cancer in combination with other agents in ... It uses recombinant protein-based technology for Sanofi's flu vaccine along with GSK's pandemic technology and was seeking ... As of 2013[update], Sanofi was in a race with Amgen and Pfizer to win approval for a drug that inhibits PCSK9, a protein that ...

https://en.wikipedia.org/wiki/Sanofi

Disc Disease by Using Stand Alone Anterior Lumbar Interbody Fusion Cages and Recombinant Human Bone Morphogenetic Protein-2: as ... of Anterior Lumbar Interbody Arthrodesis with Use of Interbody Fusion Cages and recombinant Human Bone Morphogenetic Protein-2 ... of Anterior Lumbar Interbody Arthrodesis with Use of Interbody Fusion Cages and Recombinant Human Bone Morphogenetic Protein-2 ... He performed the first hybrid multi-level cervical artificial disc replacement with spinal fusion in the country. He founded a ...

https://en.wikipedia.org/wiki/Thomas_Schuler

... is a recombinant fusion protein that is used as a treatment in adults with transfusion dependent beta thalassemia. It consists ... Washed red cells have been removed of plasma proteins that would have become a target of the patient's antibodies allowing the ... DNA contains both the instructions (genes) for stringing amino acids together into proteins, as well as stretches of DNA that ... Hemoblogin's normal alpha and beta subunits each have an iron-containing central portion (heme) that allows the protein chain ...

https://en.wikipedia.org/wiki/Beta_thalassemia

The use of dual tapered threaded fusion cages and recombinant human bone morphogenetic protein-2 on an absorbable collagen ... Bone morphogenetic protein 2 is shown to stimulate the production of bone. Recombinant human protein (rhBMP-2) is currently ... Carragee EJ, Hurwitz EL, Weiner BK (June 2011). "A critical review of recombinant human bone morphogenetic protein-2 trials in ... Subach BR, Haid RW, Rodts GE, Kaiser MG (2001). "Bone morphogenetic protein in spinal fusion: overview and clinical update". ...

https://en.wikipedia.org/wiki/Bone_morphogenetic_protein_2

... on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular ... Determined with a New Recombinant Fusion Protein Enzyme-Linked Immunosorbent Assay". Clinical and Vaccine Immunology. 20 (10): ... "Roles of the Putative Integrin-Binding Motif of the Human Metapneumovirus Fusion (F) Protein in Cell-Cell Fusion, Viral ... The HMPV fusion (F) protein encodes an RGD (Arg-Gly-Asp) motif that engages RGD-binding integrins as cellular receptors, then ...

https://en.wikipedia.org/wiki/Human_metapneumovirus

... (alb-IFN, trade name Albuferon) is a recombinant fusion protein drug consisting of interferon alpha (IFN-α) ... November 2002). "Pharmacokinetic and pharmacodynamic studies of a human serum albumin-interferon-alpha fusion protein in ...

https://en.wikipedia.org/wiki/Albinterferon

Many genetic sequences are expressed in a bacteriophage library in the form of fusions with the bacteriophage coat protein, so ... "Recombinant human Fab fragments neutralize human type 1 immunodeficiency virus in vitro". Proceedings of the National Academy ... Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that ... a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on ...

https://en.wikipedia.org/wiki/Phage_display

Their current goal is expression of orally bioavailable recombinant proteins in tobacco and lettuce chloroplasts. Thomas Alva ... Extensive recombination of chloroplast genomes after chloroplast fusion confirmed homologous recombination in chloroplasts, ... of the total leaf protein. Importantly, the insecticidal protein could be translated from the bacterial AU-rich mRNA, while for ... The Maliga lab characterised plastid promoters in vivo and in vitro, and identified proteins that are parts of the plastid PEP ...

https://en.wikipedia.org/wiki/Pal_Maliga

... and affinity purification of NE fusion proteins (i.e. affinity column). The DNA sequence of NE-tag is as follows: 5' ACC AAA ... quantification and purification of recombinant protein. This patented peptide sequence is composed of eighteen hydrophilic ... This short peptide does not adopt any significant homology to any existing proteins found in nature. This synthetic NE peptide ... This is advantageous to offer stringent specificity to the NE-tagged proteins, which are readily to be detected, quantitated, ...

https://en.wikipedia.org/wiki/NE-tag

"Genetic assembly and selective toxicity of diphtheria-toxin-related polypeptide hormone fusion proteins". Biochemical Society ... the term "toxin" means the toxic material or product of plants, animals, microorganisms ...or a recombinant or synthesized ... Toxins occur especially as a protein or conjugated protein. The term toxin was first used by organic chemist Ludwig Brieger ( ... Toxins can be small molecules, peptides, or proteins that are capable of causing disease on contact with or absorption by body ...

https://en.wikipedia.org/wiki/Toxin

... receptor protein-tyrosine kinase - recombinant fusion protein - recombinant interferon-gamma - recombinant protein - ... protein - protein biosynthesis - Protein Data Bank - protein design - protein expression - protein folding - protein isoform - ... protein P16 - protein P34cdc2 - protein precursor - protein structure prediction - protein subunit - protein synthesis - ... fusion oncogene protein G protein - G protein-coupled receptor - G3P - GABA - GABA receptor - GABA-A receptor - gag-onc fusion ...

https://en.wikipedia.org/wiki/Index_of_biochemistry_articles

... and N proteins of the virus, virus vector vaccines that have recombinant hantavirus proteins inserted in them, and virus-like ... Gc-mediated membrane fusion with the endosomal membrane, triggered by low pH, releases the nucleocapsid into the cytoplasm. ... The L protein produces nascent genomes by replication via a positive-sense RNA intermediate. Hantavirus virions are believed to ... The lipid bilayer of the viral envelope is about 5 nm thick and is embedded with viral surface proteins to which sugar residues ...

https://en.wikipedia.org/wiki/Orthohantavirus

BR3-Fc, a recombinant fusion protein built with the extracellular ligand-binding portion of BAFF-R, blocks activation of this ... Other drugs addressing B lymphocyte hyperactivity include atacicept, a recombinant fusion protein that is built with the ... who jointly published a paper detailing their findings in May 1999 and named the protein TALL-1. The same protein was named ... isolation of over one thousand different antibodies to a single protein, BLyS". J. Mol. Biol. 334 (1): 103-18. doi:10.1016/j. ...

https://en.wikipedia.org/wiki/Belimumab

The archaeal proteins used in these processes are extremely similar to Eukaryotic proteins and so are studied primarily as a ... H. volcanii cells can undergo a pairwise process of genetic exchange which involves cell fusion resulting in a heterodiploid ... Subsequently, the cells separate, giving rise to recombinant cells. The conditions Haloferax volcanii survives in, high ... Due to the salt in method cytoplasmic proteins are structured to fold in the presence of high ionic concentrations. As such, ...

https://en.wikipedia.org/wiki/Haloferax_volcanii

... in which recombinant fusion proteins are bound to calmodulin beads and eluted out by adding EGTA. EGTA is often employed in ... EGTA is used as a compound in elution buffer in the protein purification technique known as tandem affinity purification, ...

https://en.wikipedia.org/wiki/EGTA_(chemical)

... is a protein that in humans is encoded by the CTSF gene. Cysteine cathepsins are a family of cysteine proteases ... Wex T, Wex H, Brömme D (2000). "The human cathepsin F gene--a fusion product between an ancestral cathepsin and cystatin gene ... Experiments preformed showed that by using Recombinant DNA techniques with Cathepsin F cysteine protease, can create far more ... CTSF encodes a predicted protein of 484 amino acids that contains a 19-residue signal peptide. Cathepsin F contains five ...

https://en.wikipedia.org/wiki/Cathepsin_F

Synapto-pHluorin is a technique that relies on a fusion protein that combines a synaptic vesicle membrane protein and a pH ... Selective breeding - Organisms, often mice, may be bred selectively among inbred strains to create a recombinant congenic ... permits spatial and temporal control of G protein signaling in vivo. These systems utilize G protein-coupled receptors (GPCR) ... Upon synaptic vesicle release, the chimeric protein is exposed to the higher pH of the synaptic cleft, causing a measurable ...

https://en.wikipedia.org/wiki/Behavioral_neuroscience

Hybrid Fusion protein Gene pool Gene flow Introgression Nucleic acid hybridization Mouse models of breast cancer metastasis " ... A 2009 study found recombinant proteins in 3.1% and 1.8% of samples, most commonly in southeast Mexico. Seed and grain import ... Also, 5.0% of corn seed lots in Mexican corn stocks expressed recombinant proteins despite the moratorium on GM crops. In 2011 ... a protein coding sequence (usually derived from the cDNA for the protein of interest), and a stop sequence. These are typically ...

https://en.wikipedia.org/wiki/Transgene

Furthermore, cleavage of the HA protein into HA1, the binding subunit, and HA2, the fusion subunit, is performed by different ... Other manufacturing methods include an MDCK cell culture-based inactivated vaccine and a recombinant subunit vaccine ... The matrix protein (M1) and membrane protein (M2) share a segment, as do the non-structural protein (NS1) and the nuclear ... disrupting internal protein-protein interactions to release RNPs into the host cell's cytosol. The M1 protein shell surrounding ...

https://en.wikipedia.org/wiki/Influenza

Although E2 participates in cell attachment, it is not yet known which protein causes membrane fusion. The bovine viral ... Asfor, A.S.; Wakeley, P.R.; Drew, T.W.; Paton, D.J. (August 2014). "Recombinant pestivirus E2 glycoproteins prevent viral ... Npro (p20), a protein specific to pestivirus with a molecular weight of roughly 20 kDa, is the first protein generated from the ... Viral proteins are first translated as polyprotein, and then processed into individual structure and non-structure proteins by ...

https://en.wikipedia.org/wiki/Pestivirus

"Structural and functional characterization of recombinant human cellular retinaldehyde-binding protein". Protein Science. 7 (3 ... The 11-cis chromophore is then signalled back into photoreceptor cells, where it undergoes fusion with a free opsin molecule to ... Cellular retinol-binding protein, cellular retinoic acid-binding protein and cellular retinaldehyde-binding protein(CRALBP). ... Retinaldehyde-binding protein 1 (RLBP1) also known as cellular retinaldehyde-binding protein (CRALBP) is a 36-kD water-soluble ...

https://en.wikipedia.org/wiki/Retinaldehyde-binding_protein_1

The most abundant protein produced is the nucleoprotein, whose concentration in the cell determines when the RdRp switches from ... The filovirus life cycle begins with virion attachment to specific cell-surface receptors, followed by fusion of the virion ... "A Recombinant Rabies Virus Expressing the Marburg Virus Glycoprotein Is Dependent upon Antibody-Mediated Cellular Cytotoxicity ... Newly synthesized structural proteins and genomes self-assemble and accumulate near the inside of the cell membrane. Virions ...

https://en.wikipedia.org/wiki/Filoviridae

TMPRSS2 is needed to cleave the spike protein for viral fusion to cell membrane. However, a recent study has demonstrated that ... The further growing or serial passaging of this recombinant virus can demonstrate how the virus evolves or mutates to support ... VSV does not cause disease in humans which renders it a useful backbone to hold the an important protein of Zaire Ebola virus. ... In response to the virus production in the cell, the host cell can release a protein such as cytokine like interferon (IFN) ...

https://en.wikipedia.org/wiki/Endothelial_cell_tropism

FCoV type II is a recombinant virus type I with spike genes (S protein) replacement from FCoV by the canine coronavirus (CCoV) ... It is an important facilitating fusion factor of any viruses to its host cell which has been very well studied for flu. ... Coronaviruses are covered with several types of "S proteins" (or E2) forming a crown of protein spikes on the surface of the ... This ancestor gradually evolved into FCoV I. An S protein from a yet-unknown virus was passed into the ancestor and gave rise ...

https://en.wikipedia.org/wiki/Feline_coronavirus

ISBN 978-1-904455-35-6. Gupta A, Meng X, Zhu LJ, Lawson ND, Wolfe SA (January 2011). "Zinc finger protein-dependent and - ... Kim YG, Cha J, Chandrasegaran S (February 1996). "Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain". ... Recombinant adeno-associated virus (rAAV) based genome engineering is a genome editing platform centered on the use of ... It has also been used to optimize bioproducer cell lines for the biomanufacturing of protein vaccines and therapeutics. In ...

https://en.wikipedia.org/wiki/Recombinant_AAV_mediated_genome_engineering

... binds to the prefusion conformation of the RSV fusion protein, i.e. it binds to the site at which the virus would ... Nirsevimab, sold under the brand name Beyfortus, is a human recombinant monoclonal antibody with activity against respiratory ... Nirsevimab is designed to bind to the fusion protein on the surface of the RSV virus. The most common side effects reported for ...

https://en.wikipedia.org/wiki/Nirsevimab

Single cell profiling of potentiated phospho-protein networks in cancer cells (2004) Causal protein-signaling networks derived ... Schubarth, Cromwell (16 April 2021). "Biomea Fusion, Akoya Biosciences raise $285 million in IPOs". BizJournals. Retrieved 9 ... Episomal vectors rapidly and stably produce high-titer recombinant retrovirus (1996) ... proof that the NFAT transcription factor is both a REL protein and a key determinant in HIV replication, and develop of ...

https://en.wikipedia.org/wiki/Garry_Nolan

... redirected tropism by antibody-binding sites incorporated on the virion surface as a glycoprotein D fusion protein". Gene ... "The oncolytic effect of recombinant vesicular stomatitis virus is enhanced by expression of the fusion cytosine deaminase/ ... which encode easily identifiable protein markers. One example of such proteins is GFP (green fluorescent protein) which, when ... The low pathogenicity of this virus is due in large part to its sensitivity to interferons, a class of proteins that are ...

https://en.wikipedia.org/wiki/Oncolytic_virus

... providing a recombinant source of this protein for Haemophilia B patients who had previously relied on the hazardous blood- ... It was also by means of cell fusion that Harris and Goss devised the first systematic method for determining the order of genes ... With John Watkins he developed the technique of cell fusion for the study of the physiology and genetics of higher cells. They ... Rescue of influenza A virus from recombinant DNA. Fodor E, Devenish L, Engelhardt OG, Palese P, Brownlee GG, García-Sastre A. J ...

https://en.wikipedia.org/wiki/Sir_William_Dunn_School_of_Pathology

"Entrez Gene: GCS1 glucosidase I". Goodman CD, McFadden GI (2008). "Gamete Fusion: Key Protein Identified". Current Biology. 18 ... "Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human ... Montefiori DC, Robinson WE, Mitchell WM (1988). "Role of protein N-glycosylation in pathogenesis of human immunodeficiency ... 1989). "Glycosylation and processing of the human immunodeficiency virus type 1 envelope protein". J. Acquir. Immune Defic. ...

https://en.wikipedia.org/wiki/GCS1

recombinant virus release rep An abbreviation for replication protein. replication Any of the various processes by which a ... Common examples of CPEs include rounding of the infected cell, fusion with adjacent cells to form syncytia, and the appearance ... virus attachment protein Any protein which helps to facilitate the binding of a virus to a receptor on a host cell. virus ... viral plaque viral protein viral shedding viral transformation viral vector viremia virion A singular, stable particle that is ...

https://en.wikipedia.org/wiki/Glossary_of_virology

The gene fusion produces a chimeric protein segment from which a new antigenic peptide can be derived. The recognition of ... protein or recombinant viruses harbouring a sequence coding for the antigen. Clinical responses have been observed in a small ... WT1 (Wilms Tumor protein 1) is an antigen expressed in a restricted set of normal cells including renal cells and hematopoietic ... Some point mutations result in an amino-acid change in a protein. This can result in the presentation of a new peptide by an ...

https://en.wikipedia.org/wiki/Tumor_antigens_recognized_by_T_lymphocytes

"Recombinant G-protein beta gamma-subunits activate the muscarinic-gated atrial potassium channel". Nature. 368 (6468): 255-7. ... interaction of components following cell-cell and membrane-cell fusion". Journal of Cyclic Nucleotide Research. 3 (4): 227-38. ... G proteins are a vital intermediary between the extracellular activation of receptors (G protein-coupled receptors) on the cell ... This was due to loss of proteins in these cancer cells. When he introduced the missing protein from normal cells into the ...

https://en.wikipedia.org/wiki/Alfred_G._Gilman

Antibody-drug conjugate Genetic engineering Host cell protein List of pharmaceutical companies List of recombinant proteins ... Receptor constructs (fusion proteins), usually based on a naturally occurring receptor linked to the immunoglobulin frame. In ... recombinant therapeutic protein, and living medicines used in cell therapy. Biologics can be composed of sugars, proteins, ... These medications are usually one of three types: Substances that are (nearly) identical to the body's key signaling proteins. ...

https://en.wikipedia.org/wiki/Biopharmaceutical

111In-Labeled recombinant gelonin toxin-B lymphocyte stimulator protein fusion protein Arvind Chopra 1 ... 111In-Labeled recombinant gelonin toxin-B lymphocyte stimulator protein fusion protein Arvind Chopra. ... Recently a recombinant Gel (rGel)-BLyS fusion protein (rGel/BLyS) was constructed by linking rGel to the N-terminus of BLyS, ... Because of its specificity of binding to B cells, BLyS-derived fusion toxins such as the gelonin (Gel)-BLyS fusion protein has ...

https://pubmed.ncbi.nlm.nih.gov/21473028

Antihemophilic Factor (Recombinant [Fc-VWF-XTEN Fusion Protein] - Last updated on April 10, 2023. All rights owned and reserved ...

https://www.mskcc.org/cancer-care/patient-education/medications/antihemophilic-factor-recombinant-fc-vwf-xten-fusion-protein

A designed recombinant fusion protein for targeted delivery of siRNA to the mouse brain Mohamed Mohamed Haroon 1 , Ghulam ... A designed recombinant fusion protein for targeted delivery of siRNA to the mouse brain Mohamed Mohamed Haroon et al. J Control ... The designed fusion protein designated as TARBP-BTP consists of a double-stranded RNA-binding domain (dsRBD) of human Trans ... Delivery of BACE1 siRNA mediated by TARBP-BTP fusion protein reduces β-amyloid deposits in a transgenic mouse model of ...

http://www.ncbi.nlm.nih.gov/pubmed/26948382

Recombinant), FC Fusion Protein] that Sanofi Genzyme provides to U.S. patients and their families. ...

https://www.sanofi.us/en/products-and-resources/patient-services/eloctate-support

The recombinant mycobacterium tuberculosis fusion protein ESAT6-CFP10 skin test (ECST) is a novel test for tuberculosis (TB) ... Utility of recombinant fusion protein ESAT6-CFP10 skin test for differential diagnosis of active tuberculosis: A prospective ... Utility of recombinant fusion protein ESAT6-CFP10 skin test for differential diagnosis of ...

https://pesquisa.bvsalud.org/portal/resource/es/mdl-37180124

In vivo transduction of central neurons using recombinant Sindbis virus: Golgi-like labeling of dendrites and axons with ... Paucity of enkephalin production in neostriatal striosomal neurons: analysis with preproenkephalin-green fluorescent protein ... Immunocytochemical characterization and subcellular localization of human myristoyl-CoA: protein N-myristoyltransferase in HeLa ...

http://mrcanu.pharm.ox.ac.uk/research-and-techniques/recombinant-fusion-proteins.html

INDICATION: ALPROLIX® [Coagulation Factor IX (Recombinant), Fc Fusion Protein] is an injectable medicine that is used to help ... ALPROLIX® [Coagulation Factor IX (Recombinant), Fc Fusion Protein] is an injectable medicine that is used to help control and ... ALPROLIX® [Coagulation Factor IX (Recombinant), Fc Fusion Protein] is an injectable medicine that is used to help control and ... Recombinant), Fc Fusion Protein]? ...

https://www.alprolix.com/index

Activation of Recombinant Diphtheria Toxin Fusion Proteins by Specific Proteases Highly Expressed on the Surface of Tumor Cells ... Activation of Recombinant Diphtheria Toxin Fusion Proteins by Specific Proteases Highly Expressed on the Surface of Tumor Cells ... This invention relates to diphtheria toxin fusion proteins comprising a diphtheria toxin (DT) cell-killing component and a cell ... these fusion proteins bind preferentially to these cancer cells. A key feature is that these toxins are altered so as to ...

http://www.techtransfer.nih.gov/tech/tab-875

Recombinant Fusion Proteins / metabolism * Saccharomyces cerevisiae * Stereoisomerism * Trans-Activators / antagonists & ... Recombinant Fusion Proteins / genetics * ...

https://pubmed.ncbi.nlm.nih.gov/9783588/?dopt=Abstract

Aflibercept inhibits vascular endothelial growth factor (VEGF). Aflibercept is a large protein molecule with a molecular weight ... Recombinant Fusion Proteins. Disclaimer: Information presented in this database is not meant as a substitute for professional ... Aflibercept inhibits vascular endothelial growth factor (VEGF). Aflibercept is a large protein molecule with a molecular weight ...

https://ncbi.nlm.nih.gov/books/n/lactmed/LM1246

D12 - Amino Acids, Peptides, and Proteins. Chimeric Proteins. Recombinant Fusion Proteins. Complement 3 Convertase. Complement ...

https://decs.bvs.br/I/del_i2006.htm

ALTUVIIIO [antihemophilic factor (recombinant), Fc-VWF-XTEN fusion protein-ehtl] is a recombinant DNA-derived, Factor VIII ... ALTUVIIIO is a recombinant FVIII analogue fusion protein that is independent of endogenous VWF in order to overcome the half- ... ALTUVIIIO [antihemophilic factor (recombinant), Fc-VWF-XTEN fusion protein-ehtl] is a sterile, non-pyrogenic, white to off- ... ALTUVIIIO [antihemophilic factor (recombinant), Fc-VWF-XTEN fusion protein-ehtl] is a von Willebrand Factor (VWF) independent ...

https://dailymed.nlm.nih.gov/dailymed/archives/fdaDrugInfo.cfm?archiveid=711206

111In-Labeled recombinant gelonin toxin-B lymphocyte stimulator protein fusion protein. Chopra A. Chopra A. 2011 Feb 22 [ ... Pharmacokinetics of recombinant bifunctional fusion proteins. Chen X, Zaro JL, Shen WC. Chen X, et al. Expert Opin Drug Metab ... fusion protein. Materials and methods: rGel/BLyS was labeled with In-111 through DTPA with a labeling efficiency ,95%. ... The rGel/BLyS fusion toxin inhibits diffuse large B-cell lymphoma growth in vitro and in vivo. Lyu MA, Rai D, Ahn KS, Sung B, ...

https://pubmed.ncbi.nlm.nih.gov/20686856

IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG- ... IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG- ... IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG- ... IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG- ...

https://okayama.elsevierpure.com/en/publications/recombinant-mycobacterium-bovis-bacillus-calmette-gu

The fusion gene was co-expressed in ecotropic … ... against a hapten with that encoding the viral envelope protein ... Recombinant Fusion Proteins / genetics Actions. * Search in PubMed * Search in MeSH * Add to Search ... The fusion gene was co-expressed in ecotropic retroviral packaging cells with a retroviral plasmid carrying the neomycin ... Furthermore the hapten-binding particles were able to transfer the neo gene and the antibody-envelope fusion gene to mouse ...

https://pubmed.ncbi.nlm.nih.gov/8385311

Recombinant Fusion Proteins; Repressor Proteins/genetics*; Transcriptional Activation/genetics*; Transgenes/genetics* ... MeSH Terms: Actins/genetics; Animals; Bacterial Proteins/genetics*; Base Sequence; Codon/genetics; DNA Methylation; Escherichia ... coli Proteins*; Escherichia coli/genetics; Gene Expression Regulation/genetics*; Humans; Lac Repressors; Mice; Mice, Transgenic ...

https://tools.niehs.nih.gov/portfolio/index.cfm?do=portfolio.publicationDetail&id=311496

Other Name: Recombinant fusion protein. Outcome Measures. Go to Top of Page Study Description Study Design Arms and ... APG101 is a fusion protein (similar to an antibody) and will be administered as a weekly infusion. Patients can stay in this ...

https://clinicaltrials.gov/ct2/show/NCT01071837

Recombinant factor VIII Fc fusion protein for the treatment of severe haemophilia A: Final results from the ASPIRE extension ... Phase 3 study of recombinant factor VIII Fc fusion protein in severe hemophilia A. Blood. 2014 Jan 16. 123(3):317-25. [QxMD ... First-generation recombinant products: Produced in mammalian cell lines, contain animal and/or human plasma-derived proteins in ... Third-generation recombinant products: Produced in mammalian cell lines, contain no animal and/or human plasma-derived proteins ...

http://emedicine.medscape.com/article/201319-overview

Purified recombinant human p34 fusion protein. not designated. Homo Sapiens. *Homo Sapiens ...

https://discover.nci.nih.gov/abminer/antibodyDetail.do?antibodyId=246

Antibody fusion proteins: anti-CD22 recombinant immunotoxin moxetumomab pasudotox.. Kreitman RJ; Pastan I. Clin Cancer Res; ... 5. Phase I trial of anti-CD22 recombinant immunotoxin moxetumomab pasudotox (CAT-8015 or HA22) in patients with hairy cell ...

https://pubmedhh.nlm.nih.gov/biomarkers/search.php?id=31944549&mod=related&page=1&outid=&proj=

GI5005: recombinant yeast transfected with HCV NS3-core fusion protein (Globeimmune). II (2009). 66 patients with chronic HCV- ... recombinant protein, DNA and vector-based vaccines have all been explored with varying degrees of success. Recombinant protein ... Table 1. Recombinant protein hepatitis C vaccine studies.. Structure (Investigator). Phase (year). Subjects. Outcome. Ref.. ... Recombinant protein hepatitis C vaccine advantages: well tolerated with low toxicity; induces cross-neutralizing antibodies; ...

https://www.medscape.com/viewarticle/743681_12

Recombinant Fusion Proteins. 1. 1996. 3844. 0.020. Why? beta-Lactamases. 1. 1990. 372. 0.020. Why? ...

https://connects.catalyst.harvard.edu/Profiles/display/person/45804/network/researchareas/details

Recombinant luciferase-tagged SARS-CoV-2 Spike RBD protein for antibody screening by ELISA and LIPS. Delta Variant B.1.617.2. ... Recombinant RBD fusion protein (B.1.617.2 variant - Indian origin) for ELISA & LIPS. RBD-LuciaV8 (B.1.617.2) (~52 kDa) is a ... RBD-LuciaV8 (B.1.617.2) fusion protein for ELISA & LIPS InvivoGen also offers:. • REGN-10933 & REGN-10987-derived antibodies. ... Lyophilized protein should be stored at -20°C. Resuspended protein is stable for up to 1 month when stored at 4°C, and 1 year ...

https://www.invivogen.com/b16172-rbd-lucia

Recombinant Fusion Protein VEGF-IgG1. Intravenous. Aug. 3, 2012 In Use. 00024-5840-01. 00024-5840 ziv-Aflibercept. Zaltrap. ... Recombinant Fusion Protein VEGF-IgG1. Intravenous. Aug. 3, 2012 In Use. 00024-5840-03. 00024-5840 ziv-Aflibercept. Zaltrap. ... Recombinant Fusion Protein VEGF-IgG1. Intravenous. Aug. 3, 2012 In Use. 79952-0110-01. 79952-0110 loncastuximab tesirine. ...

https://seer.cancer.gov/oncologytoolbox/canmed/ndconc/?s=-product__proprietary_name&p=8

Endogenous protein tagging for absolute quantification provides a powerful extra dimension to protein analysis, allowing the ... but also enabled biochemical analysis through efficient recovery of the protein complexes wherein the tagged proteins reside. ... The endogenous use of these tags for detailed protein characterization is widespread in lower organisms that allow for ... With the recent advances in genome engineering, tagging of endogenous proteins is now within reach for most experimental ...

https://www.nature.com/articles/srep27220?error=cookies_not_supported&code=d623a101-b97d-4270-bd36-82b4eae03b18

Bivalent anti-human myostatin adnectin recombinant human IgG1-Fc fusion protein : Notification of discontinuation of a ... Bivalent anti-human myostatin adnectin recombinant human IgG1-Fc fusion protein (RO7239361) ... of an agreed paediatric investigation plan for bivalent anti-human myostatin adnectin recombinant human IgG1-Fc fusion protein ...

https://www.ema.europa.eu/en/medicines/human/paediatric-investigation-plans/emea-001793-pip01-15-m03

Application of Recombinant Fusion Proteins for Tissue Engineering Masato Nagaoka, Hu-Lin Jiang, Takashi Hoshiba, Toshihiro ... Application of Recombinant Fusion Proteins for Tissue Engineering Masato Nagaoka, Hu-Lin Jiang, Takashi Hoshiba, Toshihiro ... Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for ... Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for ...

https://researchmap.jp/read0069256

The antibodies did not appear to recognize the epitope 63-68 in the recombinant fusion protein. We identified an immunodominant ... eliminate the IgG binding for each epitope and single and multiple mutations were expressed as recombinant GST fusion proteins ... Antibody recognition of the mutant proteins was determined by inhibition ELISA. All three mAbs recognized the same aa sequence ... Inhibition ELISA with the mutant proteins indicated that epitope 126-131 was the dominant epitope, but mutation of epitope 120- ...

http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=%27ergonom%2A%27+or+musculoskeletal+or+repetitive+or+carpal&f1=TI&Startyear=&t1=1&s2=%27ergonom%2A%27+or+musculoskeletal+or+repetitive+or+carpal&terms=3&Adv=1&ct=&B1=Search&f2=KW&Limit=500&Sort=DP+DESC&D1=10&EndYear=&PageNo=285&RecNo=2843&View=f&

We fused the gene encoding an antibody fragment directed against a hapten with that encoding the viral envelope protein (Pr80env) of the ecotropic Moloney murine leukemia virus. (nih.gov)
Furthermore the hapten-binding particles were able to transfer the neo gene and the antibody-envelope fusion gene to mouse fibroblasts. (nih.gov)
In principle, the display of antibody fragments on the surface of recombinant retroviral particles could be used to target virus to cells for gene delivery, or to retain the virus in target tissues. (nih.gov)
APG101 is a fusion protein (similar to an antibody) and will be administered as a weekly infusion. (clinicaltrials.gov)
15. Antibody fusion proteins: anti-CD22 recombinant immunotoxin moxetumomab pasudotox. (nih.gov)
Recombinant protein vaccines that induce anti-envelope antibody responses are unlikely to provide sterilizing immunity owing to the genetic variability of the HCV envelope region - but may yet play a role in attenuating the course of primary infection or serve as an adjunct to a T-cell-based vaccine. (medscape.com)
Protein levels can be obtained by antibody-based approaches such as classical enzyme-linked immunosorbent assays (ELISA) experiments with theoretical detection and quantification limits in the zeptomolar concentration range 7 . (nature.com)
Antibody recognition of the mutant proteins was determined by inhibition ELISA. (cdc.gov)
The NIAID Laboratory of Malaria Immunology and Vaccinology (LMIV), Vaccine Development Unit, has an open position for a malaria vaccine staff scientist to lead LMIV's Recombinant Antigen and Antibody Development Unit. (nih.gov)

In this work, we describe the selection of peptides with ideal mass spectrometry characteristics for use in quantification of tagged proteins using targeted proteomics. (nature.com)
We mined the proteome of the hyperthermophile Pyrococcus furiosus to obtain two peptides that are unique in the proteomes of all known model organisms (proteotypic) and allow sensitive quantification of target proteins in a complex background. (nature.com)
By combining these 'Proteotypic peptides for Quantification by SRM' (PQS peptides) with epitope tags, we demonstrate their use in co-immunoprecipitation experiments upon transfection of protein pairs, or after introduction of these tags in the endogenous proteins through genome engineering. (nature.com)

The designed fusion protein designated as TARBP-BTP consists of a double-stranded RNA-binding domain (dsRBD) of human Trans Activation response element (TAR) RNA Binding Protein (TARBP2) fused to a brain targeting peptide that binds to monosialoganglioside GM1. (nih.gov)

LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins. (invivogen.com)
A conserved oligomerization domain in the disordered linker of coronavirus nucleocapsid proteins. (nih.gov)

6. Expression, purification, and immobilization of recombinant tamavidin 2 fusion proteins. (nih.gov)
7. Tamavidin, a versatile affinity tag for protein purification and immobilization. (nih.gov)
They enable the detection of the protein in the cell and allow the purification of the associated protein complex. (nature.com)

We here constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). (elsevierpure.com)

Luciferase-tagged RBD proteins are ideal for studying the binding of anti-spike monoclonal antibodies (mAbs) by solid-phase ELISA and/or solution‑phase LIPS assays, as well as anti‑spike polyclonal antibodies in the sera of recovered COVID‑19 patients and/or vaccinees by LIPS [1-3]. (invivogen.com)

The endogenous use of these tags for detailed protein characterization is widespread in lower organisms that allow for efficient homologous recombination. (nature.com)
With the recent advances in genome engineering, tagging of endogenous proteins is now within reach for most experimental systems, including mammalian cell lines cultures. (nature.com)
Endogenous protein tagging for absolute quantification provides a powerful extra dimension to protein analysis, allowing the detailed characterization of endogenous proteins. (nature.com)
In rat and mouse fibroblasts, green fluorescent protein chimera and endogenous RhoG proteins colocalize according to a tubular cytoplasmic pattern, with perinuclear accumulation and local concentration at the plasma membrane. (cnrs.fr)
RhoG-dependent events are not mediated through a direct interaction with Rac1 and Cdc42Hs targets such as PAK-1, POR1, or WASP proteins but require endogenous Rac1 and Cdc42Hs activities: coexpression of a dominant negative Rac1 impairs membrane ruffling and lamellipodia but not filopodia or microvilli formation. (cnrs.fr)

The use of protein tagging to facilitate detailed characterization of target proteins has not only revolutionized cell biology, but also enabled biochemical analysis through efficient recovery of the protein complexes wherein the tagged proteins reside. (nature.com)
The incumbent will work in a NIAID laboratory that designs and tests malaria vaccine candidates for transmission blocking and pregnancy malaria using biochemistry, immunology, and microbiology tools to examine proteins, protein complexes, fusion proteins, and preclinical model responses. (nih.gov)
Characterization of DNA-protein complexes by nanoparticle tracking analysis and their association with systemic lupus erythematosus. (nih.gov)

10. Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli. (nih.gov)
12. Facile Synthesis and Characterization of a Novel Tamavidin-Luciferase Reporter Fusion Protein for Universal Signaling Applications. (nih.gov)

Antibodies to ribosomal P2 protein were rarely detected, ransmission routes of human parvovirus 4 (PARV4), except in patients with systemic lupus erythematosus ( 5 ). (cdc.gov)
20. Burkavidin: a novel secreted biotin-binding protein from the human pathogen Burkholderia pseudomallei. (nih.gov)
Recombinant fusion protein of human ACP1 (NP_009030.1). (antikoerper-online.de)
Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human RPS12 (NP_001007.2). (fishersci.com)

The control protein was ribosomal P2 protein fused to SUMO. (cdc.gov)

Aflibercept is a large protein molecule with a molecular weight of 115,000, absorption is unlikely because it is probably partly destroyed in the infant's gastrointestinal tract and poorly absorbed orally, so systemic effects in infants are not expected. (nih.gov)

9. Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties. (nih.gov)
Inhibition ELISA with the mutant proteins indicated that epitope 126-131 was the dominant epitope, but mutation of epitope 120-125 was also required to eliminate mAb reactivity to Hev b 5. (cdc.gov)
We have expressed mutant RhoG proteins fused to the green fluorescent protein and analyzed subsequent changes in cell surface morphology and modifications of cytoskeletal structures. (cnrs.fr)

RBD‑LuciaV8 (B.1.617.2) has been generated by recombinant DNA technology, produced in CHO cells, and purified by IMAC (Immobilized Metal Affinity Chromatography) using a C‑terminal histidine tag. (invivogen.com)

The antibodies did not appear to recognize the epitope 63-68 in the recombinant fusion protein. (cdc.gov)

Expression of chimeric envelope proteins in helper cell lines and integration into Moloney murine leukemia virus particles. (nih.gov)

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. (nih.gov)

Because of its specificity of binding to B cells, BLyS-derived fusion toxins such as the gelonin (Gel)-BLyS fusion protein has been studied by investigators to selectively target and treat B cell malignancies (3-6). (nih.gov)
However, mammalian cells can internalize the toxin in association with a carrier, at which point Gel is lethal to the cells because it inhibits protein synthesis completely within 3-4 days of internalization and release within the cell. (nih.gov)
therefore, these fusion proteins bind preferentially to these cancer cells. (nih.gov)
The fusion gene was co-expressed in ecotropic retroviral packaging cells with a retroviral plasmid carrying the neomycin phosphotransferase gene (neo), and retroviral particles with specific hapten binding activities were recovered. (nih.gov)
Daybue is a twice daily oral solution that works by reducing inflammation in the brain, stopping certain types of cells from becoming overactive, and increasing the amount of the naturally occurring protein called IGF-1. (primetherapeutics.com)
They promote cell membrane fusion and thereby may function in the uptake of the virus by cells. (nih.gov)
Microtubule depolymerization upon nocodazole treatment leads to a loss of RhoG protein from the cell periphery associated with a reversal of the RhoG phenotype, whereas PDGF or bradykinin stimulation of nocodazole-treated cells could still promote Rac1- and Cdc42Hs-dependent cytoskeletal reorganization. (cnrs.fr)

Recombinant protein hepatitis C vaccine studies. (medscape.com)

Site-directed mutagenesis was used to selectively eliminate the IgG binding for each epitope and single and multiple mutations were expressed as recombinant GST fusion proteins. (cdc.gov)

They were named viral protein (VP) 2 and in Taiwan, we detected DNA in plasma of 3 mothers and their newborns with hydrops. (cdc.gov)

The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. (caslab.com)

17. Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation. (nih.gov)

272-630 and aa 604-914 of ORF2, were fused to bacterial and Chien-Ching Hung small ubiquitin-like modifi er (SUMO) protein (a member of a ubiquitin-like protein family) and used as antigens in In studying the epidemiology of parvovirus 4 (PARV4) immunoblot. (cdc.gov)

Utility of recombinant fusion protein ESAT6-CFP10 skin test for differential diagnosis of active tuberculosis: A prospective study. (bvsalud.org)
Constitutively active RhoG proteins produce morphological and cytoskeletal changes similar to those elicited by a simultaneous activation of Rac1 and Cdc42Hs, i.e., the formation of ruffles, lamellipodia, filopodia, and partial loss of stress fibers. (cnrs.fr)

This invention relates to diphtheria toxin fusion proteins comprising a diphtheria toxin (DT) cell-killing component and a cell-binding component such as granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin 2 (IL-2), or epidermal growth factor (EGF). (nih.gov)
5. Phase I trial of anti-CD22 recombinant immunotoxin moxetumomab pasudotox (CAT-8015 or HA22) in patients with hairy cell leukemia. (nih.gov)

However, none of these tags allow for a direct quantification of the protein. (nature.com)

Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein. (nih.gov)

Anatomy10

Organisms11

Diseases1

Chemicals and Drugs41

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes28

Information Science3

VEGF is required for growth and survival in neonatal mice. (1/41883)

Cell polarization: chemotaxis gets CRACKing. (2/41883)

Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization. (3/41883)

Bcl-2 regulates amplification of caspase activation by cytochrome c. (4/41883)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (5/41883)

B-MYB transactivates its own promoter through SP1-binding sites. (6/41883)

A single membrane-embedded negative charge is critical for recognizing positively charged drugs by the Escherichia coli multidrug resistance protein MdfA. (7/41883)

Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers. (8/41883)

Recombinant Fusion Proteins

Molecular Sequence Data

Amino Acid Sequence

Recombinant Proteins

Cloning, Molecular

Escherichia coli

Base Sequence

Vaccines, Synthetic

Immunotoxins

Membrane Fusion

Epitopes

Viral Fusion Proteins

Cell Fusion

Genetic Vectors

DNA, Complementary

Immunoconjugates

Cell Line

Protein Binding

Sequence Homology, Amino Acid

Glutathione Transferase

Antibodies, Monoclonal

Enzyme-Linked Immunosorbent Assay

Gene Expression

Plasmids

DNA Primers

Mice, Inbred BALB C

Blotting, Western

Binding Sites

Immunoblotting

Electrophoresis, Polyacrylamide Gel

Bacterial Proteins

Protozoan Proteins

Antibody Specificity

Immunoglobulin G

Sequence Alignment

Diphtheria Toxin

Membrane Glycoproteins

Antigens, Bacterial

Tumor Cells, Cultured

Polymerase Chain Reaction

Antibodies, Bacterial

Autoantibodies

Oncogene Proteins, Fusion

Kinetics

Molecular Weight

Rabbits

Cattle

Gene Fusion

Spinal Fusion

T-Lymphocytes

ADP Ribose Transferases

Protein Structure, Tertiary

Transfection

DNA, Recombinant

Green Fluorescent Proteins

Peptide Fragments

Membrane Fusion Proteins

Cells, Cultured

Luminescent Proteins

Bacterial Toxins

Mutation

Cricetinae

Maltose-Binding Proteins

Membrane Proteins

Baculoviridae

Viral Envelope Proteins

beta-Galactosidase

Recombination, Genetic

Carrier Proteins

Cercopithecus aethiops

Viral Proteins

Oncogene Fusion

Mutagenesis, Site-Directed

Protein Engineering

CHO Cells

Cell Membrane

HeLa Cells

Spodoptera

DNA-Binding Proteins

Transcription Factors