Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins prepared by recombinant DNA technology.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Semisynthetic conjugates of various toxic molecules, including RADIOACTIVE ISOTOPES and bacterial or plant toxins, with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; and ANTIGENS. The antitumor or antiviral immune substance carries the toxin to the tumor or infected cell where the toxin exerts its poisonous effect.
The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes.
Sites on an antigen that interact with specific antibodies.
Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.
Established cell cultures that have the potential to propagate indefinitely.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.
Antibodies produced by a single clone of cells.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Proteins found in any species of bacterium.
Proteins found in any species of protozoan.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.
Glycoproteins found on the membrane or surface of cells.
Substances elaborated by bacteria that have antigenic activity.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.
The rate dynamics in chemical or physical systems.
The sum of the weight of all the atoms in a molecule.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology.
Operative immobilization or ankylosis of two or more vertebrae by fusion of the vertebral bodies with a short bone graft or often with diskectomy or laminectomy. (From Blauvelt & Nelson, A Manual of Orthopaedic Terminology, 5th ed, p236; Dorland, 28th ed)
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Proteins that catalyze MEMBRANE FUSION.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Periplasmic proteins that bind MALTOSE and maltodextrin. They take part in the maltose transport system of BACTERIA.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Transport proteins that carry specific substances in the blood or across cell membranes.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Proteins found in any species of virus.
The GENETIC RECOMBINATION of the parts of two or more GENES, including an ONCOGENE as at least one of the fusion partners. Such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The functional hereditary units of BACTERIA.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Deletion of sequences of nucleic acids from the genetic material of an individual.
A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Multinucleated masses produced by the fusion of many cells; often associated with viral infections. In AIDS, they are induced when the envelope glycoprotein of the HIV virus binds to the CD4 antigen of uninfected neighboring T4 cells. The resulting syncytium leads to cell death and thus may account for the cytopathic effect of the virus.
A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.

VEGF is required for growth and survival in neonatal mice. (1/41883)

We employed two independent approaches to inactivate the angiogenic protein VEGF in newborn mice: inducible, Cre-loxP- mediated gene targeting, or administration of mFlt(1-3)-IgG, a soluble VEGF receptor chimeric protein. Partial inhibition of VEGF achieved by inducible gene targeting resulted in increased mortality, stunted body growth and impaired organ development, most notably of the liver. Administration of mFlt(1-3)-IgG, which achieves a higher degree of VEGF inhibition, resulted in nearly complete growth arrest and lethality. Ultrastructural analysis documented alterations in endothelial and other cell types. Histological and biochemical changes consistent with liver and renal failure were observed. Endothelial cells isolated from the liver of mFlt(1-3)-IgG-treated neonates demonstrated an increased apoptotic index, indicating that VEGF is required not only for proliferation but also for survival of endothelial cells. However, such treatment resulted in less significant alterations as the animal matured, and the dependence on VEGF was eventually lost some time after the fourth postnatal week. Administration of mFlt(1-3)-IgG to juvenile mice failed to induce apoptosis in liver endothelial cells. Thus, VEGF is essential for growth and survival in early postnatal life. However, in the fully developed animal, VEGF is likely to be involved primarily in active angiogenesis processes such as corpus luteum development.  (+info)

Cell polarization: chemotaxis gets CRACKing. (2/41883)

An early stage in the establishment of cell polarity during chemotaxis of Dictyostelium dicoideum has been identified by a recent study; the new results also show that the development of cell polarity does not rely upon cytoskeletal rearrangement, and may use a spatial sensing mechanism.  (+info)

Deletion analysis of the Drosophila Inscuteable protein reveals domains for cortical localization and asymmetric localization. (3/41883)

The Drosophila Inscuteable protein acts as a key regulator of asymmetric cell division during the development of the nervous system [1] [2]. In neuroblasts, Inscuteable localizes into an apical cortical crescent during late interphase and most of mitosis. During mitosis, Inscuteable is required for the correct apical-basal orientation of the mitotic spindle and for the asymmetric segregation of the proteins Numb [3] [4] [5], Prospero [5] [6] [7] and Miranda [8] [9] into the basal daughter cell. When Inscuteable is ectopically expressed in epidermal cells, which normally orient their mitotic spindle parallel to the embryo surface, these cells reorient their mitotic spindle and divide perpendicularly to the surface [1]. Like the Inscuteable protein, the inscuteable RNA is asymmetrically localized [10]. We show here that inscuteable RNA localization is not required for Inscuteable protein localization. We found that a central 364 amino acid domain - the Inscuteable asymmetry domain - was necessary and sufficient for Inscuteable localization and function. Within this domain, a separate 100 amino acid region was required for asymmetric localization along the cortex, whereas a 158 amino acid region directed localization to the cell cortex. The same 158 amino acid fragment could localize asymmetrically when coexpressed with the full-length protein, however, and could bind to Inscuteable in vitro, suggesting that this domain may be involved in the self-association of Inscuteable in vivo.  (+info)

Bcl-2 regulates amplification of caspase activation by cytochrome c. (4/41883)

Caspases, a family of specific proteases, have central roles in apoptosis [1]. Caspase activation in response to diverse apoptotic stimuli involves the relocalisation of cytochrome c from mitochondria to the cytoplasm where it stimulates the proteolytic processing of caspase precursors. Cytochrome c release is controlled by members of the Bcl-2 family of apoptosis regulators [2] [3]. The anti-apoptotic members Bcl-2 and Bcl-xL may also control caspase activation independently of cytochrome c relocalisation or may inhibit a positive feedback mechanism [4] [5] [6] [7]. Here, we investigate the role of Bcl-2 family proteins in the regulation of caspase activation using a model cell-free system. We found that Bcl-2 and Bcl-xL set a threshold in the amount of cytochrome c required to activate caspases, even in soluble extracts lacking mitochondria. Addition of dATP (which stimulates the procaspase-processing factor Apaf-1 [8] [9]) overcame inhibition of caspase activation by Bcl-2, but did not prevent the control of cytochrome c release from mitochondria by Bcl-2. Cytochrome c release was accelerated by active caspase-3 and this positive feedback was negatively regulated by Bcl-2. These results provide evidence for a mechanism to amplify caspase activation that is suppressed at several distinct steps by Bcl-2, even after cytochrome c is released from mitochondria.  (+info)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (5/41883)

In COS cells, Ral GDP dissociation stimulator (RalGDS)-induced Ral activation was stimulated by RasG12V or a Rap1/Ras chimera in which the N-terminal region of Rap1 was ligated to the C-terminal region of Ras but not by Rap1G12V or a Ras/Rap1 chimera in which the N-terminal region of Ras was ligated to the C-terminal region of Rap1, although RalGDS interacted with these small GTP-binding proteins. When RasG12V, Ral and the Rap1/Ras chimera were individually expressed in NIH3T3 cells, they localized to the plasma membrane. Rap1Q63E and the Ras/Rap1 chimera were detected in the perinuclear region. When RalGDS was expressed alone, it was abundant in the cytoplasm. When coexpressed with RasG12V or the Rap1/Ras chimera, RalGDS was detected at the plasma membrane, whereas when coexpressed with Rap1Q63E or the Ras/Rap1 chimera, RalGDS was observed in the perinuclear region. RalGDS which was targeted to the plasma membrane by the addition of Ras farnesylation site (RalGDS-CAAX) activated Ral in the absence of RasG12V. Although RalGDS did not stimulate the dissociation of GDP from Ral in the absence of the GTP-bound form of Ras in a reconstitution assay using the liposomes, RalGDS-CAAX could stimulate it without Ras. RasG12V activated Raf-1 when they were coexpressed in Sf9 cells, whereas RasG12V did not affect the RalGDS activity. These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral, but that Rap1 does not activate Ral due to distinct subcellular localization.  (+info)

B-MYB transactivates its own promoter through SP1-binding sites. (6/41883)

B-MYB is an ubiquitous protein required for mammalian cell growth. In this report we show that B-MYB transactivates its own promoter through a 120 bp segment proximal to the transcription start site. The B-MYB-responsive element does not contain myb-binding sites and gel-shift analysis shows that SP1, but not B-MYB, protein contained in SAOS2 cell extracts binds to the 120 bp B-myb promoter fragment. B-MYB-dependent transactivation is cooperatively increased in the presence of SP1, but not SP3 overexpression. When the SP1 elements of the B-myb promoter are transferred in front of a heterologous promoter, an increased response to B-MYB results. In contrast, c-MYB, the prototype member of the Myb family, is not able to activate the luciferase construct containing the SP1 elements. With the use of an SP1-GAL4 fusion protein, we have determined that the cooperative activation occurs through the domain A of SP1. These observations suggest that B-MYB functions as a coactivator of SP1, and that diverse combinations of myb and SP1 sites may dictate the responsiveness of myb-target genes to the various members of the myb family.  (+info)

A single membrane-embedded negative charge is critical for recognizing positively charged drugs by the Escherichia coli multidrug resistance protein MdfA. (7/41883)

The nature of the broad substrate specificity phenomenon, as manifested by multidrug resistance proteins, is not yet understood. In the Escherichia coli multidrug transporter, MdfA, the hydrophobicity profile and PhoA fusion analysis have so far identified only one membrane-embedded charged amino acid residue (E26). In order to determine whether this negatively charged residue may play a role in multidrug recognition, we evaluated the expression and function of MdfA constructs mutated at this position. Replacing E26 with the positively charged residue lysine abolished the multidrug resistance activity against positively charged drugs, but retained chloramphenicol efflux and resistance. In contrast, when the negative charge was preserved in a mutant with aspartate instead of E26, chloramphenicol recognition and transport were drastically inhibited; however, the mutant exhibited almost wild-type multidrug resistance activity against lipophilic cations. These results suggest that although the negative charge at position 26 is not essential for active transport, it dictates the multidrug resistance character of MdfA. We show that such a negative charge is also found in other drug resistance transporters, and its possible significance regarding multidrug resistance is discussed.  (+info)

Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers. (8/41883)

Gene expression in higher eukaryotes appears to be regulated by specific combinations of transcription factors binding to regulatory sequences. The Ets factor PU.1 and the IRF protein Pip (IRF-4) represent a pair of interacting transcription factors implicated in regulating B cell-specific gene expression. Pip is recruited to its binding site on DNA by phosphorylated PU.1. PU.1-Pip interaction is shown to be template directed and involves two distinct protein-protein interaction surfaces: (i) the ets and IRF DNA-binding domains; and (ii) the phosphorylated PEST region of PU.1 and a lysine-requiring putative alpha-helix in Pip. Thus, a coordinated set of protein-protein and protein-DNA contacts are essential for PU.1-Pip ternary complex assembly. To analyze the function of these factors in vivo, we engineered chimeric repressors containing the ets and IRF DNA-binding domains connected by a flexible POU domain linker. When stably expressed, the wild-type fused dimer strongly repressed the expression of a rearranged immunoglobulin lambda gene, thereby establishing the functional importance of PU.1-Pip complexes in B cell gene expression. Comparative analysis of the wild-type dimer with a series of mutant dimers distinguished a gene regulated by PU.1 and Pip from one regulated by PU.1 alone. This strategy should prove generally useful in analyzing the function of interacting transcription factors in vivo, and for identifying novel genes regulated by such complexes.  (+info)

Thesis, English, Studying the role of rhBAFF and BAFF R Fc fusion protein on lymphocytes and platelets in patients with immune thrombocytopenia for Nouran Mohamed Nabil Momen
The demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by optimizing in vitro selection using phage display which moved the major bottleneck to the production and purification of recombinant antibodies in an end-user friendly format. Single chain (sc)Fv antibody fragments require additional tags for detection and are not as suitable as immunoglobulins (Ig)G in many immunoassays. In contrast, the bivalent scFv-Fc antibody format shares many properties with IgG and has a very high application compatibility. In this study transient expression of scFv-Fc antibodies in human embryonic kidney (HEK) 293 cells was optimized. Production levels of 10-20 mg/L scFv-Fc antibody were achieved in adherent HEK293T cells. Employment of HEK293-6E suspension cells expressing a truncated variant of the Epstein Barr virus (EBV) nuclear antigen (EBNA) 1 in combination with
Abstract: Allergic responses are strongly associated with Th2-type immune responses, and modulation of the skewed Th2 response toward a more balanced response is the major goal of allergen immunotherapy (IT) in allergic disorders. To achieve this goal, several approaches have been tested. The authors previously showed that a human immunoglobulin (Ig) Fcg-Fce fusion protein (GE2) that directly cross-links FceRI and FcgRIIb on human mast cells and basophils was able to inhibit degranulation, and they reasoned that human gamma-allergen fusion protein would achieve a similar inhibitory effect in an allergen-specific fashion while preserving the immunogenicity of the allergen component. Therefore, the authors constructed and developed a human-cat chimeric fusion protein composed of the human Fcg1 and the cat allergen Fel d1 (Felis domesticus) for cat allergen-specific IT. This article summarizes the therapeutic features and potential of this novel fusion protein for allergic IT.. Chimeric human ...
Phase I study of bintrafusp alfa, a bifunctional fusion protein targeting TGF-β and PD-L1, in patients with pretreated biliary tract cancer ...
The goals of this study were to build a yeast platform for the secretion of a variety of scFv/scTCR GFP fusion proteins and to understand the effects that fusion protein construction can have on intracellular fusion protein processing. A large collection of 27 GFP fusion proteins having scFv/scTCR fusion partners representative of a wide range of secretion fitnesses was analyzed. It was discovered that the fusion secretion levels were governed by scFv/scTCR secretion fitness, rather than by GFP, linker length, or fusion orientation. In addition, type III fusions were the most fluorescent with the least amount of observed degradation, and therefore, they represent the recommended construct for secretion of GFP fusions from yeast. Finally, fusion to GFP clearly affected the intracellular processing of the scFv/scTCR, and in particular helped promote the exit of mature protein from the cell. Finally, large amounts of fully active fusion protein accumulated inside the cell as a result of secretory ...
Sandwich, UK -- Levicept Ltd., an asset-centric biotechnology company focused on the development of LEVI-04, a first-in-class treatment for chronic pain indications,…
Dear netters, I am a PhD student in Japan. I am facing some problems in expressing my fusion proteins (both GST-fusions and His-tag fusions) in soluble fractions; therefore I decided to use the TNT-coupled recticulocyte system to synthesis the desired proteins. I need to use these fusion proteins for the following assay: (i) protein interaction - pull down assay (ii) in-vitro kinase assay My questions are: (i) how to purify the translated product from the TNT- coupled recticulocyte system? (ii) Are there any references for the above assays (pull down assay & in-vitro kinase assay) using both in-vitro translated fusion proteins? Your help are kindly appreciated. Thank you. sincerely, YK ...
Two murine Mabs VA1(IgG1) and VA2(IgG1) were produced against a bacterial fusion protein comprising glutathione S-transferase and five tandem repeats of the MUC1 protein. Using the immunoperoxidase staining technique, VA1 detected 46/53 and VA2 detected 48/53 breast cancers and both also reacted wit...
The invention relates to novel recombinant fusion proteins comprising two or more erythropoietin molecules. The fusion proteins can be linked by a peptide linker. The fusion proteins can be used, for example, to treat or prevent anemia in a mammal. Also disclosed are nucleotide sequences encoding the fusion proteins vectors comprising the nucleic acid sequences of the fusion proteins and host cells transfected with the vectors.
The FLAG Expression System is an established way to express, purify and detect recombinant fusion proteins. FLAG and 3xFLAG have proven utility in numerous applications such as Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure and protein localization. These small hydrophilic tags facilitate superior detection and purification of recombinant fusion proteins when using our highly specific and sensitive ANTI-FLAG® antibodies. Ideal epitope tags are small, hydrophilic and cleavable.. The FLAG expression system utilizes a short, hydrophilic 8-amino acid peptide that is fused to the recombinant protein of interest. Because of its hydrophilic nature, the FLAG peptide is likely to be located on the surface of the fusion protein. As a result, the FLAG peptide is easily accessible for cleavage by enterokinase (Ek) and for detection with antibodies. In addition, because of the small size ...
Purification tags/Protein tag,Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.
Website: http://labs.fhcrc.org/strong/. Tailoring immunotargeting and immunomodulatory reagents for the treatment of cancer. This project builds on two ongoing efforts: (1) to specifically elicit, customize and weaponize antibodies to target solid tumors induced by viruses and (2) to translate immunomodulators that target T cell co-receptor signaling pathways from canine to human patients for managing the sequelae of treatments for hematopoietic tumors. Antibodies are clinically-relevant reagents for treating various diseases, though often require chemical conjugation to generate useful diagnostics or therapeutics. We are developing novel antibody fusion proteins, adding functional modules that enable simple, plug-and-play, non-covalent incorporation of a variety of adducts. We are also developing improved versions of MHC proteins as immunogens to efficiently elicit antibodies specific for virally-infected cells. Computational refinement of initial designs is required to fully realize these ...
RATIONALE: DTGM fusion protein may be able to locate cancer cells and stop them from growing. PURPOSE: Phase I/II trial to study the effectivenes
i want to understand also how to determine which OD to start my IPTG induction in? and when to say its time to stop when OD reaches 2 ...
I clone GST fusion proteins with pGEX 4T-3 vector, I check for inserts in minipreps (OK), grow bacteria, induce them, and run lysates on SDS-PAGE. What happens is that the expressed proteins are of GST size, probably degraded. Has anyone got that kind of results before? I checked DNA, its OK, no stop codons at the ligation site.. ...
I am attemptting to make a monoclonal antibody aganist a GST fusion protein ( 34 kDa). I am wondering if anybody outthere can tell me how much purified antigen I need to use for each immunization, and how much antigen I have to use for coding the plates for screening. Thank you, Qiang ...
SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-Cov-2, as with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). Based on structural biology studies, the receptor binding domain (RBD), located in the C-terminal region of S1, can be oriented either in the up/standing or down/lying state (6). The standing state is associated with higher ...
We describe here a simple method for expression, extraction, and purification of recombinant human IgG fused to GFP in Nicotiana...
Since many years Chimerigen Laboratories, LLC (Chimerigen) develops, manufactures and markets high quality and leading edge proteins for biomedical and immunology research. One of Chimerigens specialty is the production of unique immunoglobulin based chimeric fusion proteins using advanced cellular and molecular biological techniques.
The ERM proteins, ezrin, radixin, and moesin, are involved in the actin filament/plasma membrane interaction as cross-linkers. CD44 has been identified as one of the major membrane binding partners for ERM proteins. To examine the CD44/ERM protein interaction in vitro, we produced mouse ezrin, radixin, moesin, and the glutathione-S-transferase (GST)/CD44 cytoplasmic domain fusion protein (GST-CD44cyt) by means of recombinant baculovirus infection, and constructed an in vitro assay for the binding between ERM proteins and the cytoplasmic domain of CD44. In this system, ERM proteins bound to GST-CD44cyt with high affinity (Kd of moesin was 9.3 +/- 1.6nM) at a low ionic strength, but with low affinity at a physiological ionic strength. However, in the presence of phosphoinositides (phosphatidylinositol [PI], phosphatidylinositol 4-monophosphate [4-PIP], and phosphatidylinositol 4.5-bisphosphate [4,5-PIP2]), ERM proteins bound with a relatively high affinity to GST-CD44cyt even at a physiological ...
HHUMath.-Nat. FakultätBiologieMikrobiologieMehrUstilago communityTypes of genetic engeneering5.8 C-terminal fusion with TapTag (ctt; fusion) ...
Rat CD40 / TNFRSF5 protein (8229-CD) is manufactured by R&D Systems, over 95% purity. Reproducible results in bioactivity assays. Learn More...
Human VISTA / B7-H5 / PD-1H protein (7126-B7) is manufactured by R&D Systems, over 95% purity. Reproducible results in bioactivity assays. Learn More...
new growths elsewhere in the body, a process known as metastasis that can cause cancer to spread with deadly effect, the findings showed. This is a very promising therapy that appears to be effective and non-toxic in pre-clinical experiments, said co-researcher Amato Giaccia, a professor at the Stanford University in the US. It could open up a new approach to cancer treatment, Giaccia added. Today doctors try to slow or stop metastasis with chemotherapy, but these treatments are unfortunately not very effective and have severe side effects. The researchers sought to stop metastasis by preventing two proteins - Axl and Gas6 - from interacting to initiate the spread of cancer. Axl proteins stand like bristles on the surface of cancer cells, poised to receive bio-chemical signals from Gas6 proteins. When two Gas6 proteins link with two Axls, the signals that are generated enable cancer cells to leave the original tumor site, migrate to other parts of the body and form new cancer nodules ...
In a preclinical study, researchers at the University of Southern California evaluated whether human Collagen 7 that had been produced in the lab, when applied to mouse skin, could restore the missing protein in the skin and enhance wound healing.. They found that the Collagen 7 applied to the surface of the mouse skin was incorporated stably at the newly formed junction between the dermis and epidermis of healed wounds. It accelerated wound closure by increasing the formation of new epithelium (skin). It also led to reduced scarring and had other positive impacts. ...
Introduction SNAP-Tag fusion proteins can be expressed by transient or by stable transfection. For expression of fusion proteins with the SNAP-Tag refer to instructions supplied with the SNAP-Tag plasmids
Dendritic cells (DCs) are critical in the process of inducing immunity and peripheral tolerance. This fact opened the possibility that these cells may be targets of possible manipulations aimed at producing immunotherapeutic inducers or suppressors of the immune response. Recently it was shown that antigens can be sent directly to the DCs (Hawiger et al. 2001; Bonifaz et al., 2002). When this happens in the absence of a concomitant inflammatory stimulus, the result is the induction of immune tolerance . On the other hand, when the antigen is sent to these same cells in the presence of an inflammatory stimulus, the result is the induction of a strong immune response. The targeting of antigen to DCs in vivo is accomplished by administering low doses of a recombinant chimeric protein consisting of a monoclonal antibody specific for receptors on the surface of DCs fused with the antigen of interest. Currently we are using monoclonal antibodies that have the ability to bind to the receptor DEC 205 ...
Gentaur molecular products has all kinds of products like :search , Clemente Associates Inc \ FUSION PROTEIN TAGS Anti FLAG M1 \ flgm1250 for more molecular products just contact us
Studying the role of the fusion protein MLL-AF4 in leukemogenesis with the help of siRNAs [Elektronische Ressource] / von Maria Thomas (geb. Arkhipova) : Studying the role of the fusion protein MLL-AF4 in leukemogenesis with the help of siRNAs der Fakultät für Biologie der Eberhard Karls Universität Tübingen zur Erlangung des Grades eines Doktors der Naturwissenschaften von Maria Thomas (geb. Arkhipova) aus Pushchino (Russland) vorgelegte D i s s e r t a t i o n 2007 Tag der mündlichen Prüfung: 11.10
CGEN-15001T is a novel B7/CD28-like immune checkpoint target candidate discovered by Compugen.. Studies testing the immune function of CGEN-15001T, as a membrane bound protein or using a recombinant fusion protein containing the extracellular domain of CGEN-15001T, showed it is capable of inhibiting T cell activation, promoting a Th1 to Th2 shift, and potentially inducing immune tolerance. CGEN-15001T is expressed in subpopulations of immune cells, mainly macrophages, in both tumor and normal tissue samples.. In August 2013, Compugen signed a research and discovery collaboration and license agreement with Bayer HealthCare for the development and commercialization of antibody-based therapeutics for cancer immunotherapy against CGEN-15001T. After achieving all preclinical stage milestones, this program was transferred to Bayer for further development. To date, preclinical activities are on track, and pivotal toxicity studies and GMP clinical trial material production are ongoing.. ...
Fooling the Virus: The Fusion protein as Virus-Specific Therapeutic An ACE2 A fusion protein of ACE2 with an Immunogenic Component Project ID : 47-2020-10877
USP39 Polyclonal Antibody (CAB9582)OverviewTitle:USP39 Polyclonal Antibody (CAB9582)Size:100µLCode:CAB9582Host Species:RabbitPurification:Affinity purificationIsotype:IgGBackgroundN/AImmunogen InformationImmunogen:Recombinant fusion protein containing a ...
Rabbit polyclonal Ran antibody validated for WB, ICC/IF. Referenced in 2 publications. Immunogen corresponding to recombinant fusion protein
Mouse monoclonal Livin antibody [88C570] validated for WB, Flow Cyt and tested in Human. Immunogen corresponding to recombinant fusion protein
The study is a Phase 1b open label, non-randomized, single institution clinical trial that is designed to evaluate the safety and tolerability of three repeat
RBB 001, a recombinant fusion protein, is a targeted cytoprotectant in development with Rubicon Biotechnology for the treatment of myocardial or cerebral
Many leukemias begin when a large piece of one chromosome becomes loose and reattaches to another chromosome. This can produce fusion proteins that shut down DNA repair gens and activate genes that help the cells proliferate.
C9orf125 human Fusion Protein GST from Proteintech. Produced in E.coli-derived, PET28a, with high quality purity. Cat.No. Ag14695
P27; KIP1 human Fusion Protein GST from Proteintech. Produced in E.coli-derived, PET28a, with high quality purity. Cat.No. Ag2280
CRISPR prime editing uses pegRNA and a reverse transcriptase-Cas9 nickase fusion protein to edit DNA and has low off-target rates
The recombinant Human Osteocrin is produced with N-terminal fusion of His Tag. The Human Osteocrin His-Tagged Fusion Protein is 13.6 kDa.
In vivo degradation of GFP and a GFP-HipB hybrid.GFP and GFP with C-terminal fusion to the C terminus of HipB were expressed from a pBRlacitac promoter in BW251
Protocol Add 2 µl of the substrate stock solution to 18 µl of protein sample containing a SNAP-tag fusion protein in an appropriate buffer (see notes)
TY - JOUR. T1 - Chimeric fusion proteins - Diphtheria toxin-based. AU - Frankel, A. E.. AU - Powell, B. L.. AU - Vallera, D. A.. AU - Neville D.M., Jr. PY - 2001. Y1 - 2001. N2 - Most cancer patients receive chemotherapy drugs that target DNA or the cell division apparatus. Many of these patients develop multidrug-resistant tumor cells, thus, novel methods to overcome drug resistance are needed. One approach is to target tumor cell protein synthesis. Peptide toxins, which catalytically inactivate protein synthesis, have been re-engineered to selectively bind and intoxicate tumor cells. Diphtheria toxin (DT), a member of the class of peptide toxins, has been subjected to structural and genetic analysis and protein engineering for several decades. In this review, we will examine the structure, function, synthesis and pharmacology of anticancer DT conjugates.. AB - Most cancer patients receive chemotherapy drugs that target DNA or the cell division apparatus. Many of these patients develop ...
Nine different IgG fusion proteins and one non-fusion protein, all containing sequences from the extracellular domain of either of two human TNF receptors, were compared for their ability to bind and inhibit human TNF-alpha or TNF-beta. The fusion proteins differed with respect to TNF receptor type (p55 or p75 TNF receptor), receptor valency (one, two or four receptor domains per molecule), the presence or absence of a CH1 domain in the IgG constant region, and the proportion of the extracellular domain included in the construct. In vitro TNF binding assays and cytotoxicity assays indicated that, of the constructs that bound TNF, the greatest difference in affinity and neutralizing capability was between monovalent and bivalent receptor constructs. Differences were also noted between tetravalent and bivalent versions of p55 fusion proteins, as well as between a p75 fusion protein comprising the complete extracellular domain and one lacking the C-terminal 53 amino acids of the extracellular domain. p55
Recombinant fusion proteins have become an important class of biomolecules since the invention of recombinant DNA technology. As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of stable, bioactive fusion proteins. In the development of recombinant transferrin (Tf)-fusion proteins for protein drug oral delivery, various linkers have been designed to improve the biological activity, or to achieve desired pharmacokinetic and pharmacodynamic properties. ❧ The Introduction in this dissertation first reviewed the mechanism for Tf receptor-mediated protein drug oral delivery, as well as the recombinant Tf-fusion proteins that have been constructed previously in our lab. It also covers the current knowledge of fusion protein linkers and summarizes examples for their applications. The basic function of linkers is to covalently join the functional domains in the fusion proteins. However, linkers can offer many other advantages for ...
Recombinant fusion proteins have become an important class of biomolecules since the invention of recombinant DNA technology. As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of stable, bioactive fusion proteins. In the development of recombinant transferrin (Tf)-fusion proteins for protein drug oral delivery, various linkers have been designed to improve the biological activity, or to achieve desired pharmacokinetic and pharmacodynamic properties. ❧ The Introduction in this dissertation first reviewed the mechanism for Tf receptor-mediated protein drug oral delivery, as well as the recombinant Tf-fusion proteins that have been constructed previously in our lab. It also covers the current knowledge of fusion protein linkers and summarizes examples for their applications. The basic function of linkers is to covalently join the functional domains in the fusion proteins. However, linkers can offer many other advantages for ...
TY - JOUR. T1 - Effect of SBDs position in fusion proteins on activity and binding properties. AU - Ji, Q.. AU - Vincken, J.P.. AU - Raemakers, C.J.J.M.. AU - Suurs, L.C.J.M.. AU - Visser, R.G.F.. PY - 2005. Y1 - 2005. M3 - Comment/Letter to the editor. VL - 28. SP - 1. EP - 5. JO - Journal of Nanjing Normal university: Natural Science Edition. JF - Journal of Nanjing Normal university: Natural Science Edition. SN - 1001-4616. IS - 1. ER - ...
In the present study, we developed, for the first time, a successful protocol for expression human gAd-GLP-1-A fusion protein from Escherichia coli strain BL21 (DE3). Plasmid vector PET28a was used to express this fusion protein. This vector can produce an N-terminal His-tagged protein. His-tag is often used for protein purification [11]. The affinity of the His-tag for metal ions allows the fusion product to be quickly separated from the bulk of other bacterial proteins by using metal chelate affinity chromatography [11]. Because N-terminal His-tag may influence the function of protein, we designed an enterokinase cleavage site at the 5 terminus of the gene of the gAd-GLP-1-A fusion protein, which was used to remove the His-tag [9]. In our study, most of the His-tagged fusion protein expressed from BL21 (DE3) was present in inclusion body. In order to recover its function, the fusion protein in inclusion body was refolded in urea gradient refolding buffer. And then, the refolded protein was ...
We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. Immunohistochemical and ultrastructural characterization of these cells by light and electron microscopy showed the nonparenchymal cell type to be the Ito cells, which lie in a persinusoidal position within the space of Disse. We therefore conclude that Ito cells are the nonhepatocytic source of liver Epo production. These cells show many similarities to the Epo-producing fibroblastoid interstitial cells of the kidney.
New preclinical research in animal models finds that infusing a specific protein into scar tissue after a heart attack improves and speeds up the recovery of the heart. According to the American Heart Association (AHA), around 605,000 people in the United States have a new heart attack each year, and approximately 200,000 experience a recurrent attack.. Reperfusion, which is a technique that frees up the flow of oxygen to the hearts tissue, is a common form of treatment after a heart attack. However, up to one-quarter of people who undergo reperfusion develop heart failure within a year.. So, researchers led by James Chong - an associate professor at the University of Sydney in Australia - have explored an alternative treatment that targets the scar tissue that forms after a heart attack.. Chong and colleagues evaluated the therapeutic potential of a protein therapy called recombinant human platelet-derived growth factor-AB (rhPDGF-AB).. As its name suggests, rhPDGF-AB is a recombinant growth ...
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine Leukemia- ...
The global Spinal Fusion Systems Market report offers precise analytical information about the Spinal Fusion Systems market. The market experts and proficient analysts generate the information based on the past and current situation of Spinal Fusion Systems market, various factors affecting the growth trajectory, global sales, demand, total revenue generated, and capitalization of the market. Moreover, the report delivers a summarized assessment of the impact of federal policies and regulations on market operations. It also comprises detailed information pertaining to the Spinal Fusion Systems markets current dynamics. The global Spinal Fusion Systems market acts as a huge platform that offers several opportunities for many reputed firms, organizations, manufacturers, vendors, and suppliers Medtronic, Johnson & Johnson, Zimmer Biomet, Benvenue Medical, SeaSpine Holdings Corporation, Alphatec, Orthofix, Stryker to compete with each other to become one of the globally and regionally leading ...
Fusion of the SS18 and either one of the SSX genes is a hallmark of human synovial sarcoma. The SS18 and SSX genes encode nuclear proteins that exhibit opposite transcriptional activities. The SS18 protein functions as a transcriptional coactivator and is associated with the SWI/SNF complex, whereas the SSX proteins function as transcriptional corepressors and are associated with the polycomb complex. The domains involved in these opposite transcriptional activities are retained in the SS18-SSX fusion proteins. Here, we set out to determine the direct transcriptional consequences of conditional SS18-SSX2 fusion protein expression using complementary DNA microarray-based profiling. By doing so, we identified several clusters of SS18-SSX2-responsive genes, including a group of genes involved in cholesterol synthesis, which is a general characteristic of malignancy. In addition, we identified a group of SS18-SSX2-responsive genes known to be specifically deregulated in primary synovial sarcomas, ...
Detection of Protein-Protein Interactions Using the GST Fusion Protein Pull-down Technique. Bacterially expressed glutathione S-transferase (GST)-fused proteins are used as probes to perform direct measure of protein-protein interactions and for affinity purification. Slideshow 6521621 by...
Embodiments of the present invention provide for the facile generation of a stable recombinant fusion polypeptides with intrinsic fluorescent properties. The recombinant antibodies may be suitable fo
Title: Selective Chemokine Receptor-Targeted Depletion of Pathological Cells as A Therapeutic Strategy for Inflammatory, Allergic and Autoimmune Diseases. VOLUME: 3 ISSUE: 3. Author(s):John R. McDonald. Affiliation:Osprey Pharmaceuticals Limited, 7150 Frederick, Banting, d, St. Laurent, QC H4S 2A1, Canada.. Keywords:Chemokine, fusion protein, monoclonal antibody, immunotoxin, allergic, autoimmune, inflammation, cancer, bispecific antibodies, ribosome inactivating protein. Abstract: Targeting cell surface antigens or receptors with lytic monoclonal antibodies and specific ligand-directed fusion proteins in order to eliminate cancer cells has been in development for at least forty years. More recently, leukocyte populations known to drive a host of allergic, autoimmune and inflammatory diseases have been targeted. For fusion protein constructs, a number of different classes of cellular toxins have been fused to a variety of ligands such as monoclonal antibodies, growth factors and cytokines. ...
Disclosed are compositions and methods for producing fusion proteins with reduced immunogenicity. Fusion proteins of the invention include a junction region having an amino acid change that reduces the ability of a junctional epitope to bind to MHC Class II, thereby reducing its interaction with a T-cell receptor. Methods of the invention involve analyzing, changing, or modifying one or more amino acids in the junction region of a fusion protein in order to identify a T-cell epitope and reduce its ability to interact with a T cell receptor. Compositions and methods of the invention are useful in therapy.
Elimination of regulatory T lymphocytes may provide a way to break self-tolerance and unleash the anti-tumor properties of circulating lymphocytes. The use of fusion proteins, which link cytotoxic molecules to receptor targets, provides one approach to this problem. This study examined the ability o …
Acute myeloid leukemia (AML) is a disease that is characterized by uncontrolled proliferation of clonal neoplastic cells and accumulation in the bone marrow of blasts with an impaired differentiation program. AML accounts for approximately 80% of all adult leukemias and remains the most common cause of leukemia death. Two major types of genetic events have been described that are crucial for leukemic transformation. A proposed necessary first event is disordered cell growth and upregulation of cell survival genes. The most common of these activating events were observed in the RTK Flt3, in N-Ras and K-Ras, in Kit, and sporadically in other RTKs. Alterations in myeloid transcription factors governing hematopoietic differentiation provide second necessary event for leukemogenesis. Transcription factor fusion proteins such as AML-ETO, PML-RARalpha or PLZF-RARalpha block myeloid cell differentiation by repressing target genes. In other cases, the transcription factors themselves are mutated ...
Transfection of the different EGFP fusion constructs to IMR-90 fibroblasts. A) The wild-type SAP30L concentrates in small dense bodies in the nuclei. B) The nuc
A novel biopharmaceutical, consisting of the F8 mAb (specific to a splice isoform of fibronectin) simultaneously fused to both TNF and IL2, was found to react with the majority of solid tumors and hematologic malignancies in mouse and man, but not with healthy adult tissues. The product selectively localized to neoplastic lesions in vivo, as evidenced by quantitative biodistribution studies using radioiodinated protein preparations. When the potency of the cytokine payloads was matched by a single-point mutation, the resulting fusion protein (IL2-F8-TNFmut) eradicated soft-tissue sarcomas in immunocompetent mice, which did not respond to individual antibody-cytokine fusion proteins or by standard doxorubicin treatment. Durable complete responses were also observed in mice bearing CT26, C1498, and F9 tumors. The simultaneous delivery of multiple proinflammatory payloads to the cancer site conferred protective immunity against subsequent tumor challenges. A fully human homolog of IL2-F8-TNFmut, ...
A recombinant fusion proteins (BBG2Na) comprising the central conserved website of the respiratory syncytial computer virus subgroup A (RSV-A) (Long) G protein (residues 130 to 230) and an albumin binding website of streptococcal protein G was shown previously to protect mouse top (URT) and lower (LRT) respiratory tracts against intranasal RSV challenge (U. of the implicated epitopes in the context of the whole computer virus. However, Pepscan analyses of RSV-seropositive human being sera revealed that from the murine B-cell defensive epitopes (protectopes) that mapped towards the central conserved domains had been recognized in guy. Should these murine protectopes end up being implicated in individual LRT security also, their clustering throughout the highly conserved cysteine noose region shall possess important implications for the introduction of RSV vaccines. Respiratory syncytial trojan (RSV) is an associate Rabbit polyclonal to PPAN. from the genus as well as the family members had been ...
A recombinant fusion proteins (BBG2Na) comprising the central conserved website of the respiratory syncytial computer virus subgroup A (RSV-A) (Long) G protein (residues 130 to 230) and an albumin binding website of streptococcal protein G was shown previously to protect mouse top (URT) and lower (LRT) respiratory tracts against intranasal RSV challenge (U. of the implicated epitopes in the context of the whole computer virus. However, Pepscan analyses of RSV-seropositive human being sera revealed that from the murine B-cell defensive epitopes (protectopes) that mapped towards the central conserved domains had been recognized in guy. Should these murine protectopes end up being implicated in individual LRT security also, their clustering throughout the highly conserved cysteine noose region shall possess important implications for the introduction of RSV vaccines. Respiratory syncytial trojan (RSV) is an associate Rabbit polyclonal to PPAN. from the genus as well as the family members had been ...
A no-observable-adverse-effect level is established in the Rhesus monkey for the acute administration of the HIRMAb-GDNF fusion protein. The fusion protein targeting the insulin receptor has a PK profile similar to a classical small molecule.
Mouse monoclonal antibody raised against partial synthetic protein of Pick1. Recombinant fusion protein corresponding to amino acids 101-130 of rat Pick1. (MAB11625) - Products - Abnova
Mouse monoclonal antibody raised against recombinant TFAP2C. Recombinant fusion protein corresponding to TFAP2C. (MAB7289) - Products - Abnova
Zenix SEC-300 (3 µm, 300 Å) column delivers baseline separation of Fc fusion protein, its oligomer, and impurity. With the addition of arginine, the hydrophobic interaction between Fc fusion protein and column resin is minimized. ...
Protein A affinity chromatography is traditionally used as the capture step for monoclonal antibodies (MAbs) (1,2,3). It yields high purity because only the fragment-crystallizable (Fc) region of an antibody (IgG1 or IgG2) or Fc-containing fusion protein can bind to the protein A ligand. The resulting specificity provides substantial reduction in impurities such as host cell proteins (HCPs) and DNA (4,5,6,7,8). The dynamic binding capacity of protein A chromatography resins is generally ≤40 g/L and depends highly on residence time because…. ...
Protein A affinity chromatography is traditionally used as the capture step for monoclonal antibodies (MAbs) (1,2,3). It yields high purity because only the fragment-crystallizable (Fc) region of an antibody (IgG1 or IgG2) or Fc-containing fusion protein can bind to the protein A ligand. The resulting specificity provides substantial reduction in impurities such as host cell proteins (HCPs) and DNA (4,5,6,7,8). The dynamic binding capacity of protein A chromatography resins is generally ≤40 g/L and depends highly on residence time because…. ...
In this article we analyze protein:protein interactions and enzyme activity for several fusion proteins, and show that HaloTag fusion proteins bound to HaloLink Resin maintain function.
Studies using PKC regulatory/catalytic domain chimeras have underscored the complexity of PKC functions in relation to its structural domains. In previous reports, we and others showed that certain features of isozyme-specific PKC functions could be attributed to the catalytic domain only. These include the PKC-δ-mediated PMA-induced macrophage differentiation of 32D cells (14), the tumorigenicity of PKC-ε-overexpressing NIH 3T3 cells (15), the PKC-βII-mediated growth promotion in K-562 cells (26), and the protection of PKC-δ from down-regulation induced by bryostatin 1 in NIH 3T3 cells (17). However, in some cases, both the regulatory and the catalytic domains contribute to the isoform-specific effects (15, 18, 27). Further mapping of the structural domains beyond the catalytic and regulatory regions is essential to clearly determine the function of each structural domain and to understand how individual domains interact with each other to regulate PKC function.. Previous studies on the ...
Caltag Medsystems supply a wide range of fusion proteins for cancer research, neurobiology, metabolism, apoptosis, cell biology, immunology, infectious diseases
The recombinant human ACVR1-Fc fusion protein is expressed as a 331amino acid protein consisting of Met21 - Glu123 region of ACVR1 (UniProt accession #Q04771) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.
Raw fusion protein powder experience: You have to avoid these #errors, then #successes are already possible after 17 days ✚ the big test here
POMPEIA, C; ORTIS, F; ARMELIN, M C S. Use of pex and pgex bacterial heterologous protein expression systems for recombinant oncoprotein production and antisera generation. Biotechnology and Applied Biochemistry, San Diego, v. 25, p. 207-15, 1997 ...
Get epitope-tagging technology which can provide you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. pCMV-3Tag vectors have three copies of the epitope tag in a variety of configurations to prove a stronger signal in immmunological assays.
Sigma-Aldrich offers abstracts and full-text articles by [Mingjun Liu, Jinbao Zong, Zimin Liu, Ling Li, Xu Zheng, Bin Wang, Guirong Sun].
This unit includes protocols for the analysis of the targeting properties of Fc chimeric proteins and antibodies in mice in vivo
ATCC ® 87059™ Designation: pETGEXCT TypeStrain=False Application: encodes removable tag for protein isolation expression vector vector permitting RNA synthesis in vitro vector permitting construction of fusion proteins
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Educational technology refers not only to the hardware and software used in an educational setting, but how technological resources are used to support teaching and learning. RAND researchers form multidisciplinary teams to field rigorous studies of educational technology that provide actionable information to educators, policymakers, and product developers.
Characterization of cDNAs and recombinant fusion proteins". Eur. J. Biochem. 259 (3): 618-25. doi:10.1046/j.1432-1327.1999. ... Plasminogen-related protein B is a protein that in humans is encoded by the PLGLB2 gene. GRCh38: Ensembl release 89: ...
... is a recombinant human coagulation factor VIII, Fc fusion protein (rFVIIIFc). It is produced by recombinant ... "Eloctate (antihemophilic factor- recombinant, fc fusion protein kit". DailyMed. 4 August 2020. Retrieved 3 September 2020. " ...
Kreitman RJ, Pastan I (October 2011). "Antibody fusion proteins: anti-CD22 recombinant immunotoxin moxetumomab pasudotox". ... a condition in which fluid and proteins leak out of tiny blood vessels into surrounding tissues. Symptoms of capillary leak ...
recombinant human TNF-receptor fusion protein TNF antagonist infliximab Remicade rheumatoid arthritis, ankylosing spondylitis, ... recombinant therapeutic protein, and living cells used in cell therapy. Biologics can be composed of sugars, proteins, or ... immunoglobin G1 fusion protein incompletely characterized erythropoietin Epogen anemia arising from cancer chemotherapy, ... recombinant protein stimulation of red blood cell production etanercept Enbrel rheumatoid arthritis, ankylosing spondylitis, ...
"Coagulation Factor IX (Recombinant), Fc Fusion Protein". Drug Information Portal. U.S. National Library of Medicine. " ... The factor IX protein is composed of four protein domains: the Gla domain, two tandem copies of the EGF domain and a C-terminal ... Deficiency of this protein causes haemophilia B. It was discovered in 1952 after a young boy named Stephen Christmas was found ... A structure of the two EGF domains and the trypsin-like domain was determined for the pig protein. The structure of the Gla ...
... ged fusion proteins can be expressed using standard recombinant protein expression techniques. Furthermore, there are ... Once the fusion protein has been expressed, there is a wide range of potential areas of experimentation including enzymatic ... Protein tag SNAP tag SpyTag Fluorescent proteins Los GV, Encell LP, McDougall MG, Hartzell DD, Karassina N, Zimprich C, et al ... June 2008). "HaloTag: a novel protein labeling technology for cell imaging and protein analysis". ACS Chemical Biology. 3 (6): ...
... is a fusion protein produced by recombinant DNA. It fuses the TNF receptor to the constant end of the IgG1 antibody ... Finally, they expressed the linked DNA to produce a protein that links the protein for TNF receptor 2 to the protein for IgG1 ... The prototypic fusion protein was first synthesized and shown to be highly active and unusually stable as a modality for ... The fusion protein was developed by Bruce A. Beutler, an academic researcher then at the University of Texas Southwestern ...
Einhauer A, Jungbauer A (2001). "The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins". ... FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant ... It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can ... If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an ...
1990). "Antigenicity of a recombinant Ro (SS-A) fusion protein". Arthritis Rheum. 33 (1): 102-6. doi:10.1002/art.1780330114. ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... 60 kDa SS-A/Ro ribonucleoprotein is a protein that in humans is encoded by the TROVE2 gene. GRCh38: Ensembl release 89: ... 2005). "RNA chaperone activity of protein components of human Ro RNPs". RNA. 11 (7): 1084-94. doi:10.1261/rna.7263905. PMC ...
APO010, also known as mega-FasLigand is a recombinant fusion protein. This protein was derived from the pro-apoptotic human Fas ... APO200 is a therapeutic recombinant protein that is developed from the ectodysplasin A-1 (EDA1) gene. This product is used to ... A current target is Heat Shock Protein 90 (HSP90). HSP90 is key in protein folding and maturation. TopoTarget is currently ... pg 20 The recombinant protein product targets the ErbB2/HER2 receptor, which is overexpressed in 30% of breast cancer and is ...
One of the main uses of this protein is for removing affinity tags from purified recombinant fusion proteins. The reason for ... "Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase". Anal. Biochem. 216 (2): 413- ... Kapust RB, Waugh DS (July 2000). "Controlled intracellular processing of fusion proteins by TEV protease". Protein Expr. Purif ... "Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro". Protein ...
This kind of vaccine can be realized by DNA recombinant fusion proteins expressed in E. coli. The protein MAGE-3 has already ... Clinical vaccination studies using full-length recombinant proteins have the advantage that this form of antigen potentially ... Two cohorts were analyzed: one receiving MAGE-3 protein alone, and one receiving MAGE-3 protein with adjuvant AS028. Of nine ... recombinant viral vaccines and recombinant bacterial vaccines. While most adoptive transfer experiments have been performed ...
... and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium". Applied and ... Ilk, N.; Egelseer, E.M.; Sleytr, U.B. (2011). "S-layer fusion proteins-construction principles and applications". Current ... S-layers are composed of single proteins that form a predictable lattice structure and have potential applications in ...
Sometimes recombinant fusion proteins containing a toxin and a growth factor are also referred to as recombinant immunotoxins, ... A more specific name for this latter kind of protein is recombinant fusion toxin. They were originally produced by attaching ... The toxin is usually an AB toxin, a cytotoxic protein derived from a bacterial or plant protein, from which the natural binding ... An immunotoxin is an artificial protein consisting of a targeting portion linked to a toxin. When the protein binds to that ...
The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease). Fellers ...
... is a recombinant fusion protein designed to inhibit B cells, thereby suppressing autoimmune disease. The designer ... The subcutaneously injected protein failed a phase II trial for multiple sclerosis. The trials of atacicept in people with MS ... a transmembrane receptor protein found predominantly on the surface of B cells. Like the monoclonal antibody belimumab, ... protein combines the binding site for two cytokines that regulate maturation, function, and survival of B cells - B-lymphocyte ...
... is a recombinant fusion protein consisting of a combination of CD4 and the fragment crystallizable region. It ... 2007). "Nonlinear pharmacokinetics of high-dose recombinant fusion protein CD4-IgG2 (PRO 542) observed in HIV-1-infected ... CD4 is a surface receptor for human immunodeficiency virus (HIV). The properties of the protein means that it has potential to ...
... (alb-IFN, trade name Albuferon) is a recombinant fusion protein drug consisting of interferon alpha (IFN-α) ... November 2002). "Pharmacokinetic and pharmacodynamic studies of a human serum albumin-interferon-alpha fusion protein in ...
... a recombinant tumor necrosis factor receptor:Fc fusion protein, in patients with rheumatoid arthritis receiving methotrexate". ... The development of recombinant DNA technology in the 1970s shaped the modern understanding of what constitutes as biological ... Today, biological therapy most commonly refers to the use of proteins, such as monoclonal antibodies, to regulate the immune ... Certolizumab pegol is a recombinant antigen-binding fragment antibody that is attached to a 40kDa polyethylene glycol. The ...
Zaltrap (Aflibercept), recombinant fusion protein, approved in metastatic colorectal cancer in combination with other agents in ... Zaltrap (Aflibercept), recombinant fusion protein, approved in metastatic colorectal cancer in combination with other agents in ... It uses recombinant protein-based technology for Sanofi's flu vaccine along with GSK's pandemic technology and was seeking ... As of 2013[update], Sanofi was in a race with Amgen and Pfizer to win approval for a drug that inhibits PCSK9, a protein that ...
... recombinant restriction-grade protease) cleavage site. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that ... Protein DHX8 is part of a protein complex called spliceosome, which is in charge of pre-mRNA splicing. The spliceosome has ... One example of a purification approach for DHX8 is via a protein tag called GST-His grafted onto DHX8 protein is used. The N- ... DEAH-box helicase 8, is a protein that in humans is encoded by the DHX8 gene. This protein is member of the DEAH box ...
The fusion protein which was obtained was insoluble and was easily purified. After cleaving the fusion protein, the recombinant ... A serine protease cleaves a protein at the position of a serine, to degenerate a protein. Batroxobin is comparable to the ... This recombinant enzyme had a molecular weight of 33 kDa and included the carbohydrate structure. This method of expressing it ... Guan-Ren ZH, Duan-Hao FE, Bo-Jun YU, Guo-Cai LU, Jia-Hong SH (2008). "Long-term Toxic Effect of Recombinant Batroxobin on Rats ...
Disc Disease by Using Stand Alone Anterior Lumbar Interbody Fusion Cages and Recombinant Human Bone Morphogenetic Protein-2: as ... of Anterior Lumbar Interbody Arthrodesis with Use of Interbody Fusion Cages and recombinant Human Bone Morphogenetic Protein-2 ... of Anterior Lumbar Interbody Arthrodesis with Use of Interbody Fusion Cages and Recombinant Human Bone Morphogenetic Protein-2 ... He performed the first hybrid multi-level cervical artificial disc replacement with spinal fusion in the country. He founded a ...
... is a recombinant fusion protein that is used as a treatment in adults with transfusion dependent beta thalassemia. It consists ... Washed red cells have been removed of plasma proteins that would have become a target of the patient's antibodies allowing the ... DNA contains both the instructions (genes) for stringing amino acids together into proteins, as well as stretches of DNA that ... Hemoblogin's normal alpha and beta subunits each have an iron-containing central portion (heme) that allows the protein chain ...
"Antihemophilic Factor (recombinant), Fc Fusion Protein Monograph for Professionals". Drugs.com. "Antihemophilic Factor ( ... Buckel, P. (2012). Recombinant Protein Drugs. Birkhäuser. p. 79. ISBN 978-3034883467. Archived from the original on 2017-01-09 ... "Antihemophilic Factor (Recombinant) Monograph for Professionals". Drugs.com. "Antihemophilic Factor (Recombinant), Porcine ... Damoctocog alfa pegol, a recombinant antihemophilic factor VIII Efmoroctocog alfa, a recombinant antihemophilic factor VIII ...
"TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite ... "Human immune system mice immunized with Plasmodium falciparum circumsporozoite protein induce protective human humoral immunity ... "Antibodies to Plasmodium circumsporozoite protein (CSP) inhibit sporozoite's cell traversal activity." Journal of Immunological ... "Immunogenicity of a Prime-Boost Vaccine Containing the Circumsporozoite Proteins of Plasmodium vivax in Rodents. Infection and ...
... site-directed mutagenesis of a new recombinant fusion protein". Biochemistry. 39 (32): 9687-97. doi:10.1021/bi000274z. PMID ... The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement ... The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies ... Alternatively spliced transcript variants encoding the same protein have been described. 3-hydroxybutyrate dehydrogenase ...
... is commonly included in linker regions of recombinant fusion protein constructs. Following purification of the fusion protein, ... Activated protein C inactivates factors Va and VIIIa. Binding of activated protein C to protein S leads to a modest increase in ... Thrombin bound to thrombomodulin activates protein C, an inhibitor of the coagulation cascade. The activation of protein C is ... Recombinant thrombin is available as a powder for reconstitution into aqueous solution. It can be applied topically during ...
TACI is currently being targeted for autoimmunity and B cell malignancies via atacicept, a recombinant fusion protein that ... is a protein that in humans is encoded by the TNFRSF13B gene. TNFRSF13B is a transmembrane protein of the TNF receptor ... These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B ... In humans, the gene encoding this protein is located within the Smith-Magenis syndrome region on chromosome 17. ...
Mice of the inbred strain BALB/c were immunized with recombinant human CD28-Fc fusion proteins and boosted with a B lymphoma ... The recombinant genes were transfected into Chinese hamster ovary cells and the recombinant antibody harvested from culture ... Patricia Reaney (19 March 2006). "Protein differences may explain drug reaction". Reuters. Archived from the original on 31 ...
radiology - randomized trial - rebound - receptor (immunology) - recombinant - recombinant DNA - recombinant DNA technology - ... proteins - protocol - protozoa - provirus - pruritus - pseudo-Cushing's syndrome - pseudovirion - PUBMED - pulmonary - purified ... fusion inhibitor - fusion mechanism - fusion peptide ... core protein - correlates of immunity/correlates of protection ...
In an effort to localize the amino acid motif responsible for granule targeting, we constructed additional fusion proteins and ... Recombinant TNF is used as an immunostimulant under the INN tasonermin. TNF can be produced ectopically in the setting of ... protein binding. • protease binding. • tumor necrosis factor receptor binding. • cytokine activity. • identical protein binding ... positive regulation of protein complex assembly. • protein kinase B signaling. • positive regulation of cytokine production. • ...
... matrix 1 protein), M2, NS1 (non-structural protein 1), NS2 (other name is NEP, nuclear export protein), PA, PB1 (polymerase ... The sequencing of the influenza genome and recombinant DNA technology may accelerate the generation of new vaccine strains by ... "An open receptor-binding cavity of hemagglutinin-esterase-fusion glycoprotein from newly-identified Influenza D Virus: Basis ... protein).[63] For example, the influenza A genome contains 11 genes on eight pieces of RNA, encoding for 11 proteins: ...
For discoveries concerning the nutrient-activated TOR proteins and their central role in the metabolic control of cell growth.[ ... Discoveries revealing the universal molecular machinery that orchestrates the budding and fusion of membrane vesicles-a process ... his rational voice in the debate on recombinant DNA; and his trenchant wit.[57] ... For their invention of Herceptin, the first monoclonal antibody that blocks a cancer-causing protein, and for its development ...
Vector-based therapy - Nonlytic Newcastle Disease Virus (NDV) was engineered to express recombinant IL-15 protein to generate ... fused to an IL-15 receptor α/IgG1 Fc fusion protein.[37][38] ... A recombinant vaccinia virus expressing influenza A proteins ... tyrosine phosphorylation of STAT protein. • positive regulation of tyrosine phosphorylation of STAT protein. • regulation of ... protein binding. • cytokine receptor binding. • cytokine activity. Cellular component. • cell nucleus. • integral component of ...
Several different proteins can be affected, and the specific protein that is absent or defective identifies the specific type ... Future treatment could be had by gene therapy through recombinant adeno-associated vectors.[15] ... Among the proteins affected in LGMD are α, β, γ and δ sarcoglycans. The sarcoglycanopathies could be possibly amenable to gene ... Protein MYOT (also known as TTID one of the many genes whose mutations are responsible for this condition). ...
Through translational processing, insulin is encoded as a 110 amino acid precursor but is secreted as a 51 amino acid protein.[ ... Just as in the discovery of creating insulin through the use of recombinant DNA, the ability to artificially create stem cells ... and the opening of voltage gated calcium channels causing insulin granule fusion and exocytosis.[7] ... Subsequent to protein folding, proinsulin is transported to the Golgi apparatus and enters immature insulin granules where ...
... fusion proteins containing GFP are introduced indirectly, using RNA of the construct, or directly, with the tagged protein ... Expression of the gene and fluorescence characteristics of the recombinant protein". FEBS Letters. 341 (2-3): 277-80. doi: ... Wikimedia Commons has media related to Green fluorescent proteins.. *A comprehensive article on fluorescent proteins at ... Superresolution with two fusion proteins (GFP-Snf2H and RFP-H2A), Co-localisation studies (2CLM) in the nucleus of a bone ...
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. ... Food-processing enzymes from recombinant microorganisms--a review. Regulatory Toxicology and Pharmacology. July 2006, 45 (2): ... Intraspecific hybridisation of Trichoderma pseudokoningii by anastomosis and by protoplast fusion. FEMS Microbiology Letters. ... Hydrophobins: the protein-amphiphiles of filamentous fungi. FEMS Microbiology Reviews. November 2005, 29 (5): 877-96. PMID ...
For example, a protein found in pigs can cause humans to reject their transplanted organs. This protein can be replaced by a ... "Transgenic organisms". Gene Manipulation & Recombinant DNA. University of Westminster. Retrieved June 28, 2014. Kyndt, Tina; ... protoplast fusion, and ballistic DNA injection. Transgenesis can occur in nature. Transgenic organisms are able to express ... This means that a specific DNA sequence will code for the same protein in all organisms. Due to this similarity in protein ...
As cladribine is not a recombinant biological therapy, it is not associated with the development of antibodies against the drug ... is not explained by gene or protein expression. Instead, cladribine appears to deplete the entire B cell department. However, ...
"Identification and purification of a sperm surface protein with a potential role in sperm-egg membrane fusion". J Cell Biol. ... "Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis". J Reprod ... in Australia by engineering murine zona pellucida antigens into a recombinant ectromelia virus and a recombinant ... Wang, M; JL Shi; GY Cheng; YQ Hu; C Xu (2009). "The antibody against a nuclear autoantigenic sperm protein can result in ...
... which disrupt the membrane enough to allow the fusion protein to cross the membrane. After internalization, the fusion protein ... Individual genes can be inserted into specific sites on plasmids, and recombinant plasmids can be introduced into living cells ... The first step in this proposed model is an interaction with the unfolded fusion protein (TAT) and the membrane through ... CPP mediated delivery of proteins[edit]. The development of therapeutic proteins that has presented a valuable method to treat ...
Inflammation: Contrast agents may be designed to bind to certain proteins that become expressed in inflammatory diseases such ... Unspecific cortical lesion on CT is confirmed cystic and benign with contrast-enhanced renal ultrasonography using image fusion ... Microbubbles can be conjugated to a recombinant single-chain variable fragment specific for activated glycoprotein IIb/IIIa ( ...
Bacteria that have recombinant vectors (vector + insert), will be white and not express the GFP protein, while non-recombinant ... This can lead to the expression of a fusion protein that has a functional LacZα if its structure is not disrupted. The correct ... Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ...
Painter PC, Mosher LE, Rhoads C (1982). "Low-frequency modes in the Raman spectra of proteins". Biopolymers 21 (7): 1469-72. ... Gartner TK, Podleski TR (1975). "Evidence that a membrane bound lectin mediates fusion of L6 myoblasts". Biochem Biophys Res ... "Non-glycosylated recombinant pro-concanavalin A is active without polypeptide cleavage". EMBO J. 11 (4): 1303-7. PMC 556578 ... "Low-frequency motions in protein molecules. Beta-sheet and beta-barrel". Biophys. J. 48 (2): 289-97. Bibcode:1985BpJ....48.. ...
... receptor-construct fusion protein), and, because of its modified form, cannot neutralize receptor-bound TNF-α.[27] Additionally ... Infliximab is a purified, recombinant DNA-derived chimeric human-mouse IgG monoclonal antibody that consists of mouse heavy and ... whereas the receptor fusion protein apparently lacks this capability.[28] Although the clinical significance of these ... Etanercept also binds and inhibits the action of TNF-α, but is not a monoclonal antibody (it is instead a fusion of TNF- ...
For example, if an organism is immunised against a GST-fusion protein it will produce antibodies against the fusion-protein, ... Recombinant proteins[edit]. Possibly the most common use of affinity chromatography is for the purification of recombinant ... For thoroughness the GST protein and the GST-fusion protein can each be coupled separately. The serum is initially allowed to ... Proteins with a known affinity are protein tagged in order to aid their purification. The protein may have been genetically ...
... large subunit ribosomal proteins (rpl, orange), hypothetical chloroplast open reading frame proteins (ycf, lemon), proteins ... was later suggested to be an artifact because the mutants show increased outcrossing due to organ fusion.[49][50][51] ... "New Arabidopsis recombinant inbred lines (Landsberg erecta x Nossen) reveal natural variation in phytochrome-mediated ... Secondary databases: UniProt, database of protein sequences grouping together Swiss-Prot, TrEMBL and Protein Information ...
The genetically modified organisms produced can be used to produce products such as recombinant proteins, used in medical ... Telomeres prevent fusion of the ends of neighboring chromosomes and protect chromosome ends from damage.[58] ... Interactions with proteins. All the functions of DNA depend on interactions with proteins. These protein interactions can be ... Structural proteins that bind DNA are well-understood examples of non-specific DNA-protein interactions. Within chromosomes, ...
Life-threatening Complications Associated with Recombinant Human Bone Morphogenetic Protein in Cervical Spine Fusion". fda.gov ... Lumbar fusions are the most common type of fusion performed ~ 210,000 per year. 24,000 thoracic fusions and 157,000 cervical ... Bone morphogenetic protein (rhBMP) should not be routinely used in any type of anterior cervical spine fusion, such as with ... Anterior decompression and fusion[3]. *Posterior instrumentation and fusion - many different types of hardware can be used to ...
Under certain conditions, mitochondrial fission and its associated proteins promote autophagy whereas mitochondrial fusion ... One such example was elucidated by infection with virulent and recombinant, avirulent strains of T. gondii. The intricate ... guanylate-binding proteins (GBP), immunity-related GTPase proteins (IRGs), and very large inducible GTPase proteins (VLIG). ... LC3 is a microtubule associated soluble protein found in mammalian tissues. Cytoplasmic proteins and organelles are engulfed by ...
TNF binding proteins[edit]. A TNF-α (tumor necrosis factor-alpha) binding protein is a monoclonal antibody or a circulating ... By the use of recombinant gene technology, the mouse anti-Tac antibodies have been modified, leading to the presentation of two ... protein synthesis inhibitors.. Methotrexate[edit]. Methotrexate is a folic acid analogue. It binds dihydrofolate reductase and ... It binds to FKBP1A like tacrolimus, however the complex does not inhibit calcineurin but another protein, mTOR. Therefore, ...
... mutagenesis and cell fusion techniques that do not use recombinant nucleic acids or a genetically modified organism in the ... Human cells in which some proteins are fused with green fluorescent protein to allow them to be visualised ... so that they will overexpress the desired protein. Mass quantities of the protein can then be manufactured by growing the ... Watson JD (2007). Recombinant DNA: Genes and Genomes: A Short Course. San Francisco: W.H. Freeman. ISBN 978-0-7167-2866-5. .. ...
Jacob F, Monod J (1961). "Genetic regulatory mechanisms in the synthesis of proteins". Journal of Molecular Biology. 3: 318-56 ... Fusion of haploid nuclei occurs within reproductive structures termed cleistothecia, in which the diploid zygote undergoes ... Additionally the development of recombinant DNA technology through the use of bacteria has led to the birth of modern genetic ... Crick FH, Barnett L, Brenner S, Watts-Tobin RJ (1961). "General nature of the genetic code for proteins". Nature. 192: 1227-32 ...
These mutations can be chosen in order to understand how proteins accomplish their functions, and to change or improve protein ... Fusion. Archived from the original (PDF) on 28 November 2007.. (in French) ... isolation of a DNA sequence to expedite recombinant DNA technologies involving the insertion of a DNA sequence into a plasmid, ... The pertussis toxin is a protein exotoxin that binds to cell receptors by two dimers and reacts with different cell types such ...
ISBN 978-1-55581-480-9. Feuerman, M. H.; Lee, W. T.; Pattengale, P. K.; Fan, H. (1988). "Comparison of three recombinant murine ... Rous sarcoma virus Fusion protein Fusion gene Fusion transcript Chimeric gene Bcr-abl fusion protein Oncovirus Retrovirus ... a Gag-v-Onc fusion protein from the Rous sarcoma virus is useful in illustrating the dual role that the fusion protein plays in ... The gag-onc fusion protein (also written as Gag-v-Onc, with "v" indicating that the Onc sequence resides in a viral genome) is ...
One such particular protein complex that is conserved between processes is RAD51, a well conserved recombinase protein that has ... Recombinant frequency. References[edit]. *^ Griffiths, AJF; Gelbart, WM; Miller, JH; et al. (1999). "Modern Genetic Analysis: ... Mismatch repair (MMR) proteins, for instance, are a well-known regulatory family of proteins, responsible for regulating ... The MSH4 and MSH5 proteins form a hetero-oligomeric structure (heterodimer) in yeast and humans.[16][17][18] In the yeast ...
SMN stabilisation aims at stabilising the SMNΔ7 protein, the short-lived defective protein coded by the SMN2 gene, so that it ... Spine fusion is sometimes performed in people with SMA I/II once they reach the age of 8-10 to relieve the pressure of a ... "Delivery of recombinant follistatin lessens disease severity in a mouse model of spinal muscular atrophy". Human Molecular ... The disorder is caused by a genetic defect in the SMN1 gene, which encodes SMN, a protein widely expressed in all eukaryotic ...
Recombinant [Fc Fusion Protein]). Includes indications, proper use, special instructions, precautions, and possible side ... Factor IX (Recombinant [Fc Fusion Protein]). Generic Name: Factor IX (Recombinant [Fc Fusion Protein]) (FAK tor nyne ree KOM be ... If you are allergic to factor IX (recombinant [fc fusion protein]); any part of factor IX (recombinant [fc fusion protein]); or ... fc fusion protein]) away. *Do not put factor IX (recombinant [fc fusion protein]) back in the refrigerator after it has been ...
FACTOR IX Fc FUSION PROTEIN (fak tir nine Fc FYOO zhuh n PROH teen) is used in patients with hemophilia B to help prevent and ... Factor IX Fc Fusion Protein, Recombinant injection. What is this medicine?. FACTOR IX Fc FUSION PROTEIN (fak tir nine Fc FYOO ...
... albumin fusion protein reference guide for safe and effective use from the American Society of Health-System Pharmacists (AHFS ... Circulating half-life of factor IX (recombinant), albumin fusion protein longer than that of unmodified recombinant or plasma- ... phase I results of recombinant fusion protein linking coagulation factor IX with recombinant albumin (rIX-FP). Thromb Res. 2013 ... Factor IX (Recombinant), albumin fusion protein. Class: Hemostatics. VA Class: BL500. Brands: Idelvion ...
Factor IX (Recombinant [Fc Fusion Protein]) Adult Medication. *. ... Factor IX (Recombinant [Fc Fusion Protein]). ©2018 Memorial ...
GsaL fusion protein. Ab90837 is an active full length protein produced in Baculovirus infected Sf9 cells and has been… ... Recombinant human ADRB2 + GsaL fusion protein. See all ADRB2 + GsαL fusion proteins and peptides. ... Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1. *Guanine nucleotide binding protein G( ... This fusion protein contains a thrombin cleavage site (TS) between the His tag and GsaL subunit. ...
"Recombinant Fusion Proteins" by people in Harvard Catalyst Profiles by year, and whether "Recombinant Fusion Proteins" was a ... "Recombinant Fusion Proteins" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... Recombinant Fusion Proteins*Recombinant Fusion Proteins. *Proteins, Recombinant Fusion. *Fusion Proteins, Recombinant ... Below are the most recent publications written about "Recombinant Fusion Proteins" by people in Profiles. ...
Factor IX (Recombinant [Fc Fusion Protein]) - Last updated on May 8, 2021. All rights owned and reserved by Memorial Sloan ...
GsaL fusion protein (ab90784). Please download our general protocols booklet ... There are no specific protocols for Recombinant human ADRB1 Gly389 + ... Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins G Proteins ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
Factor IX Fc fusion protein recombinant injection is used as an on-demand treatment to control or prevent bleeding episodes, ... Factor IX is a protein that is produced naturally in the body. Alprolix® is a man-made protein produced to replicate the ...
Fc Fusion Protein for Intravenous Infusion) may treat, uses, dosage, side effects, drug interactions, warnings, patient ... Fc Fusion Protein] for Intravenous Injection. DESCRIPTION. ELOCTATE, Antihemophilic Factor (Recombinant), Fc Fusion Protein, is ... ELOCTATE, Antihemophilic Factor (Recombinant), Fc Fusion Protein, is a recombinant DNA derived, antihemophilic factor indicated ... Fc fusion protein (BDD-rFVIIIFc) is the active ingredient in ELOCTATE. BDD-rFVIIIFc is a recombinant protein consisting of a B- ...
Lisa L. Wolfe, Tanya M. Shenk, Bradford Powell, and Tonie E. Rocke "ASSESSMENT OF A RECOMBINANT F1-V FUSION PROTEIN VACCINE ... ASSESSMENT OF A RECOMBINANT F1-V FUSION PROTEIN VACCINE INTENDED TO PROTECT CANADA LYNX (LYNX CANADENSIS) FROM PLAGUE. ... Lisa L. Wolfe, Tanya M. Shenk, Bradford Powell, Tonie E. Rocke "ASSESSMENT OF A RECOMBINANT F1-V FUSION PROTEIN VACCINE ... after vaccination with a recombinant F1-V fusion protein vaccine against Yersinia pestis, the bacterium that causes plague. Ten ...
The recombinant protein, R32Leu-Arg, is composed of 32 tetrapeptide repeat sequences from the circumsporozoite … ... The immunodominant repeat region of the malaria circumsporozoite protein from Plasmodium falciparum was purified from a ... recombinant Escherichia coli to study as a potential subunit vaccine. ... Assay, purification and characterization of a recombinant malaria circumsporozoite fusion protein by high-performance liquid ...
Recombinant Human Serum Albumin/Granulocyte Colony Stimulating Factor Fusion Protein for Breast Cancer Patients. The safety and ... Recombinant Human Serum Albumin/Granulocyte Colony-Stimulating Factor Fusion Protein(2.4mg(will be injected subcutaneously at ... Evaluation of the efficacy and safety of recombinant human serum albumin / granulocyte-stimulating factor fusion protein for ... Efficacy and Safety Phase IIb Study of Recombinant Human Serum Albumin/ Granulocyte Colony-Stimulating Factor Fusion Protein ...
Many proteins produced in Escherichia coli accumulate in inclusion bodies. We have systematically evaluated the parameters that ... the refolding and renaturation of enzymatically active molecules from bacterial inclusion bodies containing a recombinant ... A method for increasing the yield of properly folded recombinant fusion proteins: single-chain immunotoxins from renaturation ... USA 88, 8616-8620). Like many other recombinant proteins, B3(Fv)-PE38KDEL is produced in E. coli in inclusion bodies and must ...
The invention relates to recombinantly produced fusion polypeptides comprising antibody V¿H? and V¿L? sequences operatively ...
A Safety and Efficacy Extension Study of a Recombinant Fusion Protein Linking Coagulation Factor IX With Albumin (rIX-FP) in ... A Safety and Efficacy Extension Study of a Recombinant Fusion Protein Linking Coagulation Factor IX With Albumin (rIX-FP) in ...
Recombinant human bone morphogenetic protein-2 (rhBMP-2) is used as a bone graft substitute in spinal fusion, which unites ( ... Effectiveness and harms of recombinant human bone morphogenetic protein-2 in spine fusion: a systematic review and meta- ... Meta-analysis of trials of recombinant human bone morphogenetic protein-2: what should spine surgeons and their patients do ... Closing in on the truth about recombinant human bone morphogenetic protein-2: evidence synthesis, data sharing, peer review, ...
The primary objective of the study is to evaluate the safety of Recombinant Coagulation Factor VIII Fc Fusion Protein (rFVIIIFc ... Study to Evaluate the Safety, Efficacy, and Pharmacokinetics of Recombinant Coagulation Factor VIII Fc Fusion Protein (rFVIIIFc ... Recombinant factor VIII Fc fusion protein for the prevention and treatment of bleeding in children with severe hemophilia A. J ... and Efficacy of Recombinant Coagulation Factor VIII Fc Fusion Protein, BIIB031, in the Prevention and Treatment of Bleeding ...
Fc Fusion Protein], Lyophilized Powder for Solution For Intravenous Injection)? Learn about drug imprint, side effects, uses ( ... home drugs a-z list side effects drug center alprolix ([coagulation factor ix (recombinant), fc fusion protein], lyophilized ... Generic Name: [coagulation factor ix (recombinant), fc fusion protein], lyophilized powder for solution for intravenous ...
Recombinant Albumin Fusion Protein sEphB4-HSA in Treating Patients With Metastatic or Recurrent Solid Tumors. The safety and ... Patients receive recombinant EphB4-HSA fusion protein IV over 60 minutes on days 1 and 15. Courses repeat every 28 days in the ... Patients receive recombinant EphB4-HSA fusion protein IV over 60 minutes on days 1. Courses repeat every 21 days in the absence ... Patients receive recombinant albumin fusion protein sEphB4-HSA IV over 60 minutes on days 1, 8, 15, and 22. Courses repeat ...
A trial of etanercept, a recombinant tumor necrosis factor receptor:Fc fusion protein, in patients with rheumatoid arthritis ... Fc fusion protein (TNFR:Fc), to methotrexate therapy would provide additional benefit to patients who had persistent rheumatoid ...
The recombinant fusion proteins F1-LcrV and F1-LcrV-HSP70(II) were purified using Ni-NTA columns and formulated with alum to ... The recombinant fusion proteins F1-LcrV and F1-LcrV-HSP70(II) were purified using Ni-NTA columns and formulated with alum to ... Both the recombinant constructs caf1-lcrV and caf1-lcrV-hsp70(II) were cloned in pET28a vector and expressed in Escherichia ... Both the recombinant constructs caf1-lcrV and caf1-lcrV-hsp70(II) were cloned in pET28a vector and expressed in Escherichia ...
Production of recombinant protein bio-insecticides on a commercial scale can only be cost effective if host strains with very ... A recombinant fusion protein containing an arthropod toxin, ω-hexatoxin-Hv1a, (from funnel web spider Hadronyche versuta) ... Optimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in Pichia pastoris: sequence ... Introduction of multiple expression cassettes increased the amount of secreted recombinant fusion protein in a laboratory scale ...
We have previously developed a fusion protein with 3 copies of the ectodomain of matrix protein 2 (M2e), which is one of the ... To improve the protective ability of the self-adjuvanting fusion protein, CTA1-3M2e-DD, we incorporated it into porous ... To improve the protective ability of the self-adjuvanting fusion protein, CTA1-3M2e-DD, we incorporated it into porous ... Most importantly, immune responses to NPLs that also contained recombinant hemagglutinin (HA) were strongly enhanced in a CTA1- ...
This study is an attempt to produce a fusion protein by binding the fragment NT-gp96 in upstream of sequence of the N terminal ... The recombinant fusion protein was expressed in Escherichia coli (E. coli). Protein expression was analyzed by sodium dodecyl ... The designed recombinant fusion protein (rNT-gp96-NT300) was expressed and purified in E. coli (BL21) for the development of a ... Purification of the recombinant fusion protein under denaturing condition was performed using Ni-NTA Agarose (QIAGEN, Germany) ...
Modeling and Simulation of Production of Metallothionein and Red Fluorescent Fusion Protein by Recombinant Escherichia Coli ... Modeling and Simulation of Production of Metallothionein and Red Fluorescent Fusion Protein by Recombinant Escherichia Coli ... Modeling and Simulation of Production of Metallothionein and Red Fluorescent Fusion Protein by Recombinant Escherichia Coli ... simulations-using-labview-software/modeling-and-simulation-of-production-of-metallothionein-and-red-fluorescent-fusion-protein- ...
Factor ix albumin fusion protein recombinant (By injection). Factor IX Albumin Fusion Protein Recombinant (FAK-tor NINE al-BUE- ... You should not receive it if you had an allergic reaction to factor IX albumin fusion protein recombinant or hamster proteins. ...
Antihemophilic factor Fc fusion protein is a fully recombinant, fusion protein that temporarily replaces the missing ... Recombinant, fusion protein with antihemophilic factor linked to the Fc protein fragment of human IgG1. Used for the control ... Fc fusion protein and any potential adverse effects on the breast-fed infant from antihemophilic factor, Fc fusion protein or ... Fc fusion protein and any potential adverse effects on the breast-fed infant from antihemophilic factor, Fc fusion protein or ...
Study of Recombinant Factor IX Fc Fusion Protein (rFIXFc) in Subjects With Hemophilia B. December 7, 2010 ... Study of Recombinant Factor IX Fc Fusion Protein (rFIXFc) in Subjects With Hemophilia B ...
Creating recombinant fusion protein conjugates for targeting SNARE protease into neuroendocrine cells ... Patil, Nilesh Baliram (2016) Creating recombinant fusion protein conjugates for targeting SNARE protease into neuroendocrine ... An alternative strategy was also adopted with a view to stapling a recombinant BoNT/AΔHCC-SNAP-25 protein (without the neuronal ... The methodology involved genetic fusion and use of a protein stapling technology with a view to replacing the BoNT/A C-terminal ...
  • The immunodominant repeat region of the malaria circumsporozoite protein from Plasmodium falciparum was purified from a recombinant Escherichia coli to study as a potential subunit vaccine. (nih.gov)
  • Many proteins produced in Escherichia coli accumulate in inclusion bodies. (nih.gov)
  • The recombinant fusion protein was expressed in Escherichia coli ( E. coli ). (biopharminternational.com)
  • Modeling and Simulation of Production of Metallothionein and Red Fluorescent Fusion Protein by Recombinant Escherichia Coli Using Graphical Programming, Modeling, Programming and Simulations Using LabVIEW™ Software Riccardo De Asmundis, IntechOpen, DOI: 10.5772/14091. (intechopen.com)
  • The construction of vectors, their efficient expression in Escherichia coliand simple purification of the fusion proteins and two insulin chains are described. (jascoinc.com)
  • Although biologically active recombinant GM-CSF has been successfully produced in Escherichia coli , the reported levels are extremely poor. (springer.com)
  • Berges, H., Joseph-Liauzun, E., and Fayet, O. (1996) Combined effects of the signal sequence and the major chaperone proteins on the export of human cytokines in Escherichia coli . (springer.com)
  • Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. (biochemj.org)
  • Recombinant human phenylalanine hydroxylase (hPAH) was produced in high yields in Escherichia coli using the pET and pMAL expression vectors. (biochemj.org)
  • However, high-level expression and purification of recombinant FGF21 (rFGF21) in Escherichia coli (E. coli) is difficult because rFGF21 forms inclusion bodies in the bacteria making it difficult to purify and obtain high concentrations of bioactive rFGF21. (biomedcentral.com)
  • Non-chromatographic purification of recombinant elastin-like polypeptides and their fusions with peptides and proteins from Escherichia coli. (duke.edu)
  • These recombinant fusion proteins were expressed in Escherichia coli and Saccharomyces cerevisiae from appropriately designed plasmids. (elsevier.com)
  • Low expression and instability are significant challenges in the recombinant production of therapeutic peptides. (jascoinc.com)
  • This approach can be used for the purification of elastin-like polypeptides alone or as a purification tool for peptide or protein fusions where recombinant peptides or proteins genetically appended to elastin-like polypeptide tags can be purified without chromatography. (duke.edu)
  • This Ca 2- -dependent binding has been exploited in the development of a fusion tail approach for the affinity purification of recombinant proteins and peptides. (elsevier.com)
  • No human or animal derived proteins are used in the purification or formulation processes. (rxlist.com)
  • This assay was used in a variety of applications including measurement of production levels of the antigen during fermentation, evaluation of the protein purification process, quantitation of protein recovery, and as one criterion of protein purity. (nih.gov)
  • Incorporation of a C-terminal (His) 6 tag enabled single step purification of the fusion protein. (springer.com)
  • Baumgartner P, Harper K, Raemaekers RJ, Durieux A, Gatehouse AMR, Davies HV, Taylor MA (2003) Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris . (springer.com)
  • The current study introduces a novel expression and purification system for human insulin production using the molecular chaperone αB-crystallin (αB-Cry) as a fusion partner protein. (jascoinc.com)
  • This paper describes a low-cost and uncomplicated method for the production and purification of biologically active hbFGF fusion proteins. (figshare.com)
  • This protocol describes the purification of elastin-like polypeptides and their peptide or protein fusions and discusses basic characterization techniques to assess the thermal behavior of pure elastin-like polypeptide products. (duke.edu)
  • Enhanced throughput of antibody generation has been addressed by optimizing in vitro selection using phage display which moved the major bottleneck to the production and purification of recombinant antibodies in an end-user friendly format. (biomedcentral.com)
  • Miniaturization and parallelization of panning and screening has been established in microtiter plate scale [ 5 ] and moves the bottleneck of the antibody pipeline towards the production and purification of milligram amounts of the individual antibodies in an application compatible recombinant format. (biomedcentral.com)
  • In this application note, the capture and purification of recombinant albumin-fusion proteins using AlbuPure® is described. (astreabioseparations.com)
  • Purification of different albumin-fusion proteins (1) IL-1ra and (2) anti-HIV gp41 were performed using AlbuPure® on an automated chromatography workstation. (astreabioseparations.com)
  • Domain III (DIII) of the tick-borne encephalitis virus (TBEV) protein E contains epitopes, which induce antibodies capable of neutralizing the virus. (mdpi.com)
  • Mice immunization showed that the chimeric protein induced twice the number of anti-EIII antibodies in comparison with EIII alone. (mdpi.com)
  • Attention has been given to epitopes from the hemagglutinin (HA) stem region in order to raise neutralizing antibodies against conserved structures of the protein ( 2 - 6 ). (frontiersin.org)
  • The recombinant hPAH, recovered in soluble forms, revealed a high specific activity even in crude extracts and was detected as a homogeneous band by Western-blot analysis using affinity-purified polyclonal rabbit anti-(rat PAH) antibodies. (biochemj.org)
  • Using this recombinant protein as the antigen for ELISA, the anti Yo antibodies in the sera and CSF were examined and 3 new patients with PCD possessing anti-Yo antibody were found. (scienceopen.com)
  • GENTAUR antibody-antibodies.com The Marketplace for Antibodies : [Mechanisms of recombinant adenovirus-mediated SD-HA fusion protein proliferation inhibition and induced apoptosis of K562 cells]. (antibody-antibodies.com)
  • Transient production of recombinant scFv-Fc antibodies in HEK293-6E in combination with optimized vectors and fed batch shake flasks cultivation is efficient and robust, and integrates well into a high-throughput recombinant antibody generation pipeline. (biomedcentral.com)
  • These recombinant antibodies have affinities in the low nanomolar range in some cases even in the subnanomolar range without any additional engineering [ 3 ]. (biomedcentral.com)
  • The E-Fc proteins were secreted as soluble homodimers, which retained reactivity with anti-TBEV and anti-rabbit IgG antibodies. (hokudai.ac.jp)
  • The E-Fc proteins were then used to develop an enzyme-linked immunosorbent assay (ELISA) to detect TBEV antibodies in rodent and human sera. (hokudai.ac.jp)
  • Most flavivirus cross-reactive antibodies are produced against the highly conserved fusion loop (FL) domain in the viral envelope (E) protein. (rki.de)
  • An additional advantage of this fusion system was tested in the generation of mutacin-specific polyclonal antibodies. (openmicrobiologyjournal.com)
  • Like many other recombinant proteins, B3(Fv)-PE38KDEL is produced in E. coli in inclusion bodies and must be denatured and refolded to become active. (nih.gov)
  • Results indicated that the NT-gp96-NT300 recombinant fusion protein was successfully produced in a prokaryotic ( E. coli ) host. (biopharminternational.com)
  • An alternative strategy was also adopted with a view to stapling a recombinant BoNT/AΔHCC-SNAP-25 protein (without the neuronal binding domain) with Vamp2.βNGF produced in E. coli, and a synthetic syntaxin-1 peptide via SNARE complex formation. (dcu.ie)
  • In this paper,fermentation condition of the engineering bacterial E.coli JM109 which expressed recombinant human thrombopoietin/glutathione (rhTPO/GST) fusion protein was investigated.Conditions that affect fermentation of the bacterial were tested and optimized.How the fermentation condition affect the quantity of whole exogenous protein the engineering bacterial expressed was also discussed.Result showed that using complex culture medium,adding organic materials and inducing with isopropyl. (cnki.com.cn)
  • In the pMAL system, hPAH was fused through the target sequences of the restriction protease factor Xa (IEGR) or enterokinase (D4K) to the C-terminal end of the highly expressed E. coli maltose-binding protein (MBP). (biochemj.org)
  • Both N- and C- terminus fusion strategies were used to assemble constructs of the MLB chain fused to GFP in the pGFPuv expression vector which was transformed into BL21 E. coli and the expression of the target gene/fusion was successfully carried out. (salford.ac.uk)
  • A fusion protein which comprises the B subunit of the labile toxin (LT-B) of E. coli and part of the flagellin (flaA) protein of C. jejuni is antigenic and is useful for decreasing colonization in chickens by Campylobacter species. (usda.gov)
  • The protein is produced by E. coli cells, transformed by the plasmid pBEB into which DNA sequences encoding the novel protein have been introduced. (usda.gov)
  • ScFv-Fc fusion protein was expressed at high level over 30% of total host cell proteins in E.coli. (bvsalud.org)
  • The short peptide was expressed directly as a fusion protein with a superfolder form of the green florescent protein ( sf GFP), resulting in a high yield expression of soluble sf GFP-mutacin fusion protein (30 kDa) in the cytoplasm of E. coli. (openmicrobiologyjournal.com)
  • ELISA, differential scanning calorimetry, intrinsic fluorescence, and computational analysis were applied for the characterization of the immunogenicity and conformation of the chimeric protein. (mdpi.com)
  • In the present study, we describe the identification and molecular characterization of a surface protein of M. leprae , ML-LBP21, that binds peripheral nerve laminin-2. (pnas.org)
  • Biophysical characterization and stabilization of the recombinant albumin fusion protein sEphB4-HSA. (semanticscholar.org)
  • ADVATE ® [Antihemophilic Factor (Recombinant)] is a registered trademark of Baxalta Incorporated, a Takeda company. (eloctate.com)
  • 2 ELOCTATE ® [Antihemophilic Factor (Recombinant), Fc Fusion Protein] Package Insert. (eloctate.com)
  • what is the most important information I should know about ELOCTATE ® [Antihemophilic Factor (Recombinant), Fc Fusion Protein]? (eloctate.com)
  • In the phase 3 B-LONG (Recombinant Factor IX Fc Fusion Protein [rFIXFc] in Subjects With Haemophilia B) study, rFIXFc demonstrated a prolonged half-life compared with recombinant factor IX (rFIX), and safety and efficacy for prophylaxis and treatment of bleeding in subjects with moderately-severe to severe haemophilia B. In this B-LONG sub-analysis, rFIXFc was evaluated for efficacy in subjects requiring major surgery. (elsevier.com)
  • These modifications did not affect the insecticidal activity of the recombinant toxin towards lepidopteran larvae. (springer.com)
  • As part of an ongoing restoration program in Colorado, USA, we evaluated adverse reactions and seroconversion in captive Canada lynx ( Lynx canadensis ) after vaccination with a recombinant F1-V fusion protein vaccine against Yersinia pestis , the bacterium that causes plague. (bioone.org)
  • Although our findings suggest that the F1-V fusion protein vaccine evaluated here is likely to stimulate antibody responses that may help protect Canada lynx from plague, we observed no apparent differences in survival between vaccinated and unvaccinated subject animals. (bioone.org)
  • The formation of this vaccine construction was accompanied by significant conformational changes in the chimeric protein. (mdpi.com)
  • We have previously developed a fusion protein with 3 copies of the ectodomain of matrix protein 2 (M2e), which is one of the most explored conserved influenza A virus antigens for a broadly protective vaccine known today. (frontiersin.org)
  • Previous studies showed that non-structural proteins of hepatitis C virus (HCV) such as the NS5B protein could be a potent target for designing new therapeutic vaccine to stimulate the immune system. (biopharminternational.com)
  • This fusion protein can be used as a novel vaccine candidate against HCV, if it is immunogenicity approved by additional immunological assays. (biopharminternational.com)
  • In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. (epfl.ch)
  • If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells. (epfl.ch)
  • Findings in this and our earlier studies define the bivalent fusion protein rVE as a potent candidate vaccine molecule with the capability to concurrently stimulate humoral and cell mediated immune responses and a proof of concept for developing efficient subunit vaccines against Gram negative facultative intracellular bacterial pathogens. (uni-wuerzburg.de)
  • In this study, we evaluated a recombinant vaccine prepared from non-pathogenic Mycobacterium smegmatis (rMS) that expresses a fusion of early secreted antigenic target 6-kDa antigen (ESAT6) and culture filtrate protein 10 (CFP10). (semanticscholar.org)
  • METHODS: We compared the efficacies of the most commonly used vaccine constructs--adjuvanted protein, plasmid DNA, and live bacterial vectors--bearing the immunodominant secreted antigens early secreted antigen target-6 and antigen 85B, either alone or as a fusion protein. (mendeley.com)
  • The test group received recombinant human serum albumin / granulocyte thorns for injection Stimulant fusion protein 2.4mg treatment. (clinicaltrials.gov)
  • This study was undertaken to determine whether the addition of etanercept, a soluble tumor necrosis factor receptor (p75):Fc fusion protein (TNFR:Fc), to methotrexate therapy would provide additional benefit to patients who had persistent rheumatoid arthritis despite receiving methotrexate. (nih.gov)
  • The aim of this study was to develop a strategy for pain management by generating a biotherapeutic that requires targeting of soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor (SNARE) within the hyper-active sensory nerves. (dcu.ie)
  • Its concentration reached 30% of total protein, and exceeded 95% of all soluble proteins. (biomedcentral.com)
  • Factor IX Fc fusion protein recombinant injection is used as an on-demand treatment to control or prevent bleeding episodes, prevent bleeding during surgery, or reduce the frequency of bleeding episodes in patients with hemophilia B (congenital Factor IX deficiency). (mayoclinic.org)
  • Recombinant Human Serum Albumin/Granulocyte Colony-Stimulating Factor Fusion Protein(2.4mg(will be injected subcutaneously at at 10 am (±90 min) on the 3th and 7th day of each chemotherapy cycle.After injection, stop administrating if Absolute Neutrophil Count (ANC) in peripheral blood exceeded 5.0×109/L at two contiguous times at least. (clinicaltrials.gov)
  • Recombinant Human Granulocyte Colony-Stimulating Factor Injection (5μg/kg/day) will be injected subcutaneously at 10 am (±90 min) from the 3rd of per chemotherapy cycle. (clinicaltrials.gov)
  • On September 17, 2020, Hualan Biological Engineering Inc. received the clinical approval document of "Recombinant Exendin-4-Fc Fusion Protein Injection" issued by the National Medical Products Administration, with the approval number of 2020LP00444. (hualanbio.com)
  • The indications of Recombinant Exendin-4-Fc Fusion Protein Injection are: assisting the therapy of diet and exercise to improve the control of blood glucose for the patients with Type 2 diabetes. (hualanbio.com)
  • According to the drug registration procedure, Hualan Biological Engineering Inc. will organize the clinical trials as soon as possible in accordance with the requirements of national drug clinical trials after acquiring the clinical approval document of Recombinant Exendin-4-Fc Fusion Protein Injection, and Hualan Biological Engineering Inc. will apply the product production approval documents and GMP certificate after completing the clinical trials. (hualanbio.com)
  • To enhance the immunogenicity of this protein, which has a low molecular weight, the aim of the present work was to express, isolate, and characterize a chimeric protein based on the fusion of the bacterial chaperone HSP70 of Yersinia pseudotuberculosis and EIII (DIII + stem) as a prospective antigen for an adjuvanted delivery system, the tubular immunostimulating complex (TI-complex). (mdpi.com)
  • Prime-boost vaccination with Bacillus Calmette Guerin and a recombinant adenovirus co-expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis induces robust antigen-specific immune responses in mice. (semanticscholar.org)
  • Thus, the novel N-S fusion antigen-based IFA could be an attractive alternative to present whole-virus-based IFAs for the diagnosis of SARS-CoV infection. (nus.edu.sg)
  • We made the recombinant protein deduced from the cDNA clone encoding the leucine-zipper motif of the Yo antigen. (scienceopen.com)
  • Recombinant antigen microarrays for serum/plasma antibody detection. (scienceopen.com)
  • ORCID: https://orcid.org/0000-0002-3882-6129 2016, Novel fusion proteins based on recombinant lectins for delivery of a cytotoxic peptide specifically to cancer cells , PhD thesis, University of Salford. (salford.ac.uk)
  • We analyzed the blood loss patterns of 617 consecutive adult patients undergoing lumbar and/or thoracic fusions requiring subfascial drain placement at a single institution from January 2009 to December 2016. (asianspinejournal.org)
  • Effectiveness and harms of recombinant human bone morphogenetic protein-2 in spine fusion: a systematic review and meta-analysis. (nih.gov)
  • Recombinant human bone morphogenetic protein-2 (rhBMP-2) is used as a bone graft substitute in spinal fusion, which unites (fuses) bones in the spine. (nih.gov)
  • Meta-analysis of trials of recombinant human bone morphogenetic protein-2: what should spine surgeons and their patients do with this information? (nih.gov)
  • Closing in on the truth about recombinant human bone morphogenetic protein-2: evidence synthesis, data sharing, peer review, and reproducible research. (nih.gov)
  • Observational studies have reported serious complications associated with recombinant human bone morphogenetic protein-2 (rhBMP-2) in cervical and lumbar spine fusions. (spinesection.org)
  • Does Recombinant Human Bone Morphogenic Protein 2 Affect Perioperative Blood Loss after Lumbar and Thoracic Spinal Fusion? (asianspinejournal.org)
  • This study aimed to determine whether recombinant human bone morphogenic protein 2 (rhBMP-2) reduces total perioperative blood loss during lumbar and thoracic fusion. (asianspinejournal.org)
  • High-dose recombinant human bone morphogenetic protein-2 impacts histological and biomechanical properties of a cervical spine fusion segment: results from a sheep model. (scienceopen.com)
  • The 'off-label' use of high-dose recombinant human bone morphogenetic protein-2 (rhBMP-2) in lumbar and cervical fusion leads to heterotopic bone formation and vertebral osteolysis. (scienceopen.com)
  • Recombinant human bone morphogenetic protein-2 as an adjunct for spine fusion in a pediatric population. (scienceopen.com)
  • There are several reports, which documented a high incidence of complications following the use of recombinant human bone morphogenetic protein-2 (rhBMP-2) in anterior cervical fusions (ACFs). (e-neurospine.org)
  • We performed a retrospective analysis of 197 patients who underwent anterior cervical fusion (ACF) with the use of recombinant human bone morphogenetic protein-2 (rhBMP-2) during 2007-2012. (e-neurospine.org)
  • Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a member of this family, which has gained a widespread popularity for its application in spinal fusions. (e-neurospine.org)
  • How does recombinant human bone morphogenetic protein-4 enhance posterior spinal fusion? (eduhk.hk)
  • A rabbit posterolateral intertransverse process fusion model was used to evaluate the effect that different doses of recombinant human bone morphogenetic protein-4 delivered in a porous hydroxyapatite-tricalcium phosphate ceramic had on osteogenesis and spinal fusion. (eduhk.hk)
  • To study the biologic effect and threshold dose of recombinant human bone morphogenetic protein-4 in enhancing spinal fusion. (eduhk.hk)
  • The enhancing effects of recombinant human bone morphogenetic proteins 2 and 7 on spinal fusion have been proved, and clinical trials of their application are in progress. (eduhk.hk)
  • Recombinant human bone morphogenetic protein-4 is another osteoinductive protein that has the ability to induce heterotopic bone formation, and its potential for enhancing spinal fusion has not yet been studied. (eduhk.hk)
  • The animals were divided into four groups using different graft materials: allograft as well as hydroxyapatite-tricalcium phosphate augmented with 0, 1.25, and 5 μg of recombinant human bone morphogenetic protein-4, respectively. (eduhk.hk)
  • At week 7, ossification in the intertransverse process area ceased in groups without recombinant human bone morphogenetic protein-4, whereas active multicentric endochondral bone formation was demonstrated in groups with this growth factor. (eduhk.hk)
  • The success rate of contiguous bony bridging was found to correlate positively with the dose of recombinant human bone morphogenetic protein-4. (eduhk.hk)
  • Recombinant human bone morphogenetic protein-4 effectively enhances new bone formation and accelerates fusion in the rabbit posterolateral posterior spinal fusion model. (eduhk.hk)
  • The effective dose of recombinant human bone morphogenetic protein-4 is 10 times lower than the reported dosage of recombinant human bone morphogenetic proteins 2 and 7. (eduhk.hk)
  • Fingerprint Dive into the research topics of 'How does recombinant human bone morphogenetic protein-4 enhance posterior spinal fusion? (eduhk.hk)
  • you are allergic to hamster proteins. (rxlist.com)
  • You should not receive it if you had an allergic reaction to factor IX albumin fusion protein recombinant or hamster proteins. (stlukes-stl.com)
  • IDELVION is contraindicated in patients who have had life-threatening hypersensitivity to the product or its components, including hamster proteins. (idelvion.com)
  • Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. (abcam.com)
  • To obtain a high expressing clone of P. pastoris to produce recombinant Hv1a/GNA, a straightforward method was used to produce multi-copy expression plasmids, which does not require multiple integrations to give clones of P. pastoris containing high copy numbers of the introduced gene. (springer.com)
  • This study is an attempt to produce a fusion protein by binding the fragment NT-gp96 in upstream of sequence of the N terminal fragment (NT300) of the NS5B gene in an expression vector. (biopharminternational.com)
  • The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4L orbitally shaken bioreactors. (epfl.ch)
  • To overcome this problem we attached a signal sequence, as well as an amino-terminal His-tag fusion to the GM-CSF gene. (springer.com)
  • The sequence data showed a clear indication that the insert is our desired gene product allowing us to go on to design a fusion protein. (salford.ac.uk)
  • In the present study, the N195-Sc fusion protein was highly expressed in insect (Sf9) cells infected with a recombinant baculovirus bearing the hybrid gene under the control of a polyhedrin promoter. (nus.edu.sg)
  • Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis.This gene encodes a protein-disulfide isomerase. (antibody-antibodies.com)
  • After N-terminal amino acid sequence analysis, PCR-based strategy was used to clone the gene that encodes this protein. (pnas.org)
  • Nonetheless, from the clinical standpoint, gene transfer has limitations, including toxicity, limited control of the levels of protein expression, and difficulty in expressing more than one protein at a time. (jimmunol.org)
  • Protein transfer (or protein "painting"), which enables simultaneous delivery of well-defined amounts of multiple proteins to a cell, provides a valuable alternative means to gene transfer ( 9 , 10 ). (jimmunol.org)
  • Plasminogen-related protein B is a protein that in humans is encoded by the PLGLB2 gene. (wikipedia.org)
  • This recombinant molecule is composed of the variable domains of monoclonal antibody B3 (B3(Fv)) fused to a truncated mutant form of Pseudomonas exotoxin A (PE38KDEL). (nih.gov)
  • To investigate whether fusion protein SD-HA could regulate its downstream signaling molecule activity by competing with the phospho-BCR-ABL Y177 site, and its mechanisms to inhibit proliferation and induce apoptosis of K562 cells. (antibody-antibodies.com)
  • Deduced amino acid sequence of this M. leprae laminin-binding protein predicts a 21-kDa molecule (ML-LBP21), which is smaller than the observed molecular size in SDS/PAGE. (pnas.org)
  • anda fourth DNA molecule encoding a desired protein. (patentsencyclopedia.com)
  • 9. The fusion DNA construct of claim 1, wherein the first DNA molecule and second DNA molecule encode a Trichoderma CBH1 signal sequence and carrier protein or a Trichoderma endoglucanase signal sequence and carrier protein. (patentsencyclopedia.com)
  • 15. The fusion DNA construct of claim 3, wherein the first DNA molecule and second DNA molecule encode a CBH I signal sequence and carrier protein and the fourth DNA molecule encodes an antibody light chain or fragment thereof. (patentsencyclopedia.com)
  • Likewise, the relative immunodominance of individual antigens in the fusion molecule is altered by the choice of delivery system. (mendeley.com)
  • A large amount of the recombinant fusion proteins with high purity was obtained by applying a single step anion exchange chromatography or metal chelate affinity. (jascoinc.com)
  • In order to support the rise in the production of recombinant albumin-fusion proteins, an affinity matrix is required that is highly stable, robust, non-toxic and provides high binding capacity, recovery and purity. (astreabioseparations.com)
  • The elution fraction shows high IL-1ra albumin-fusion protein purity and recovery (lanes 7 and 8). (astreabioseparations.com)
  • High anti-HIV gp41 albumin-fusion protein purity and recovery is observed in the elution fraction (lanes 7 and 8). (astreabioseparations.com)
  • AlbuPure® can be used to capture and purify recombinant albumin-fusion proteins from cell culture supernatants with high purity and recovery, as shown in this application note. (astreabioseparations.com)
  • The resulting plasmid was transformed into DE3 cells for the heterologous expression of the chimeric protein, which was purified by immobilized metal affinity chromatography (IMAC). (mdpi.com)
  • By contrast, when expressed as a fusion protein in the pMAL system, hPAH was resistant to cleavage by host cell proteases and was conveniently purified by affinity chromatography on an amylose resin. (biochemj.org)
  • The expressed fusions proteins were purified through affinity chromatography using CNBR-activated Sepharose 4B column coupled with degalactosylated fetuin so as to confirm the sialic acid binding activity of MLB. (salford.ac.uk)
  • The fused protein was purified by DEAE sepharose FF and Ni-NTA affinity chromatography. (biomedcentral.com)
  • An affinity chromatography system has been developed for the separation of recombinant fusion proteins based on the Ca 2+ -dependent binding of calmodulin (CaM) to the drug phenothiazine. (elsevier.com)
  • In particular, the developed affinity chromatography system was used to isolate several ProtA-CaM fusion proteins. (elsevier.com)
  • The fusion proteins were purified by affinity chromatography with glutathione-resin (Amersham Biosciences). (diabetesjournals.org)
  • These fusion proteins were purified by affinity chromatography with amylose-resin (New England BioLabs). (diabetesjournals.org)
  • Recombinant Mycobacterium smegmatis expressing an ESAT6-CFP10 fusion protein induces anti-mycobacterial immune responses and protects against Mycobacterium tuberculosis challenge in mice. (semanticscholar.org)
  • Cellular and humoral immunogenicity of recombinant Mycobacterium smegmatis expressing Ag85B epitopes in mice. (semanticscholar.org)
  • Recombinant Bacillus subtilis spores for the delivery of Mycobacterium tuberculosis Ag85B-CFP10 secretory antigens. (semanticscholar.org)
  • IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein. (semanticscholar.org)
  • BDD-rFVIIIFc is purified using a series of chromatography steps, including affinity capture with a recombinant, single chain antibody fragment produced in a yeast expression system. (rxlist.com)
  • The detection of anti-Purkinje cell antibody (anti-Yo antibody) by ELISA using recombinant Yo fusion protein]. (scienceopen.com)
  • 14. The fusion DNA construct of claim 13, wherein the antibody is a light chain or heavy chain monoclonal antibody. (patentsencyclopedia.com)
  • After vector optimization and process optimization the yield of an scFv-Fc antibody and a cytotoxic antibody-RNase fusion protein further increased 3-4-fold to more than 450 mg/L. Finally, an entirely new mammalian expression vector was constructed for single step in frame cloning of scFv genes from antibody phage display libraries. (biomedcentral.com)
  • A wide range of albumin-fusion proteins (Albufuse®) have been produced to extend the circulatory half-life of the fusion partner whilst maintaining it's activity. (astreabioseparations.com)
  • The Alumedix yeast based high expression system was used to produce cell culture supernatant (CCS) containing either (1) IL-1ra or (2) gp41 albumin-fusion proteins. (astreabioseparations.com)
  • The albumin-fusion proteins were selectively eluted using buffer containing sodium octanoate. (astreabioseparations.com)
  • To prevent proteolysis, the Hv1a/GNA fusion expression construct was modified by site-directed mutagenesis to remove a potential Kex2 cleavage site at the C-terminus of the Hv1a peptide. (springer.com)
  • Fitches EC, Pyati PS, King GF, Gatehouse JA (2012) Fusion to snowdrop lectin dramatically enhances the oral activity of the insecticidal peptide ω-hexatoxin-Hv1a by mediating its delivery to the central nervous system. (springer.com)
  • In this regard, several therapies such as recombinant vaccines, synthetic peptide vaccines, DNA vaccines, and vector vaccines have been created, but they have low efficacy in treatment and viral infection removal (7, 8, 9). (biopharminternational.com)
  • Encouragingly, after inclusion of this leader peptide sequence into a Vamp2.βNGF construct, it was expressed as an active protein, although with low yield. (dcu.ie)
  • ADRB2 is a member of the G protein-coupled receptor superfamily. (abcam.com)
  • A trial of etanercept, a recombinant tumor necrosis factor receptor:Fc fusion protein, in patients with rheumatoid arthritis receiving methotrexate. (nih.gov)
  • Ablibercept is a recombinant fusion protein that acts as a decoy receptor for the ligands, vascular endothelial growth factor-A (VEGF-A) and placental growth factor (PIGF). (creativebiomart.net)
  • This study presents an approach for inhibiting p53-deficient tumor cell growth by using protein-based E4orf4 that had been genetically fused to epidermal growth factor (EGF) to ensure selective targeting of EGF receptor-overexpressing tumor cells. (ovid.com)
  • Western blot analyses of SD-HA fusion protein on cell membrane receptor pathway to death cascade caspase-8, caspase-3 and PRAP were performed. (antibody-antibodies.com)
  • The purified hbFGF proteins, both with and without the fusion tags, were biologically active, which was confirmed by their ability to stimulate proliferation of NIH3T3 cells. (figshare.com)
  • B-domain deleted recombinant Factor VIII , Fc fusion protein ( BDD -rFVIIIFc) is the active ingredient in ELOCTATE. (rxlist.com)
  • ELOCTATE is Factor VIII fused with an Fc protein. (eloctate.com)
  • To independently assess the effectiveness and harms of rhBMP-2 in spinal fusion and reporting bias in industry-sponsored journal publications. (nih.gov)
  • In spinal fusion, rhBMP-2 has no proven clinical advantage over bone graft and may be associated with important harms, making it difficult to identify clear indications for rhBMP-2. (nih.gov)
  • The authors conclude that on the basis of the currently available evidence, it is difficult to identify clear indications for rhBMP-2 in spinal fusion. (spinesection.org)
  • Medtronic Sofamor Danek, Memphis, TN, USA) has been used as an osteoinductive adjuvant to enhance spinal fusions for more than 15 years [ 1 - 3 ]. (asianspinejournal.org)
  • Therefore, in this study, we aimed to use a retrospective cohort design to examine the impact of rhBMP-2 on the total perioperative blood loss (intraoperative estimated blood loss and postoperative drain output) following lumbar and thoracic spinal fusions involving the placement of a subfascial drain. (asianspinejournal.org)
  • Our study sought to empirically test the hypothesis that the use of rhBMP-2 during spinal fusion procedures reduces intraoperative and postoperative blood loss relative to the use of ICBG. (asianspinejournal.org)
  • We hypothesized that a high dose of rhBMP-2 in cervical spinal fusion could induce substantial alterations in bone, leading to mechanical impairment. (scienceopen.com)
  • Biologic manipulation for spinal fusion is an area undergoing active research. (eduhk.hk)
  • For this study, 24 adult New Zealand white rabbits underwent single-level unilateral posterior intertransverse process spinal fusion at L5-L6. (eduhk.hk)
  • Kim YB, Zhao KT, Thompson DB, Liu DR. An anionic human protein mediates cationic liposome delivery of genome editing proteins into mammalian cells. (harvard.edu)
  • In this study, a recombinant plasmid that expresses the membrane and envelope (E) proteins of TBEV fused to the Fc domain of rabbit IgG was constructed and expressed in mammalian cells. (hokudai.ac.jp)
  • The non-structural 5 B (NS5B) protein (65 kDa, with 591 amino acids) is a RNA-dependent RNA polymerase (RdRp) that plays a key role in the replication of the HCV genome (14). (biopharminternational.com)
  • When a larger fusion partner, such as the Maltose-binding protein (MBP-tag), was used the expression levels increased further to 69.5 µg/mL, which along with the MBP-tag represented approx 12% of the total cellular protein. (springer.com)
  • SUR1 (598-1,003) was also expressed as a maltose-binding protein (MBP)-fused protein in BL21. (diabetesjournals.org)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
  • For lumbar spine fusion, rhBMP-2 and iliac crest bone graft were similar in overall success, fusion, and other effectiveness measures and in risk for any adverse event, although rates were high across interventions (77% to 93% at 24 months from surgery). (nih.gov)
  • For both lumbar and cervical fusions, rhBMP-2 and iliac crest bone graft (ICBG) had similar results for fusion. (spinesection.org)
  • Previous studies on rhBMP-2 versus iliac crest bone grafting in thoracic and lumbar fusions have yielded mixed results regarding reductions in blood loss and have largely neglected the postoperative period when analyzing total blood loss. (asianspinejournal.org)
  • Since then, many studies have reported reduction in the operative time and hospital stays, as well as improved fusion rates in patients treated with rhBMP-2 compared with those treated with autologous iliac crest bone grafting (ICBG) [ 3 , 4 ]. (asianspinejournal.org)
  • n = 8/group) was used to quantify the consequences of a high rhBMP-2 dose (6 mg rhBMP-2) on fusion tissue compared to the 'gold standard' of autologous, cancellous bone graft. (scienceopen.com)
  • Although high-dose rhBMP-2 treatment led to an advanced radiological fusion result compared to autograft treatment, heterotopic bone formation and vertebral bone resorption were induced simultaneously. (scienceopen.com)
  • Histological evaluation unveiled highly active bone-forming processes ventral to the fusion segment after 12 weeks, while radiolucent areas showed still a partial loss of regular trabecular structure, with rare signs of remodelling and restoration. (scienceopen.com)
  • Despite qualitative alteration of the trabecular bone structure within the fusion site, the massive anterior heterotopic bone formation led to a substantial increase in mechanical stiffness compared to the autograft group. (scienceopen.com)
  • A prospective randomized study of posterolateral lumbar fusion using osteogenic protein-1 (OP-1) versus local autograft with ceramic bone substitute: emphasis of surgical exploration and histologic assessment. (scienceopen.com)
  • In 1965, Urist 14) discovered a subset of protein extract, which had a significant potential of inducing new bone formation even at the non-osseous tissues. (e-neurospine.org)
  • The proteins were termed bone morphogenetic proteins (BMPs). (e-neurospine.org)
  • 4) and in their letter they proposed that the containment of low dose (1.05 mg) rhBMP-2 within the cage and placing demineralized bone matrix (DBM) putty surrounding the INFUSE ensures a controlled delivery of rhBMP-2 in the vicinity of desired fusion site, which therefore is associated with excellent fusion rates with no adverse effects related to rhBMP-2. (e-neurospine.org)
  • This fusion protein contains a thrombin cleavage site (TS) between the His tag and GsaL subunit. (abcam.com)
  • The insulin A- and B-chain were released from the fusion proteins using cyanogen bromide cleavage. (jascoinc.com)
  • After cleavage by restriction protease, factor Xa or enterokinase, hPAH was separated from uncleaved fusion protein, MBP and restriction proteases by hydroxylapatite or ion-exchange (DEAE) chromatography. (biochemj.org)
  • The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5' to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell. (patentsencyclopedia.com)
  • Mutacin was released from the fusion by enzymatic cleavage at the tobacco etch virus (TEV) protease recognition site and separated from the carrier sf GFP by nickel affinity and gel filtration chromatography. (openmicrobiologyjournal.com)
  • Fitches EC, Bell HA, Powell ME, Back E, Sargiotti C, Weaver RJ, Gatehouse JA (2010) Insecticidal activity of scorpion toxin (ButaIT) and snowdrop lectin (GNA) containing fusion proteins towards pest species of different orders. (springer.com)
  • For anterior cervical spine fusion, rhBMP-2 was associated with increased risk for wound complications and dysphagia. (nih.gov)
  • Anterior cervical fusion (ACF) is a commonly performed surgical procedure to treat degenerative disorders of the cervical spine 9) . (e-neurospine.org)
  • This study is about an experimental drug called sEphB4-HSA (recombinant albumin fusion protein sEphB4-HSA). (clinicaltrials.gov)
  • In this study we looked into the possible reasons for poor expression and found that protein toxicity coupled with protease-based degradation was the principal reason for low productivity. (springer.com)
  • Included in this study are the first recombinant PRV strains expressing C terminal VP26-mCherry. (princeton.edu)
  • In our previous study, we established that the bivalent fusion protein (rVE) comprising immunologically active regions of Y pestis LcrV (100-270 aa) and YopE (50-213 aa) proteins conferred complete passive and active protection against lethal Y enterocolitica 8081 challenge. (uni-wuerzburg.de)
  • Phase 3 study of recombinant factor IX Fc fusion protein in hemophilia B . New England Journal of Medicine , 369 (24), 2313-2323. (elsevier.com)
  • Treatment of bleeding episodes with recombinant factor VIII Fc fusion protein in A-LONG study subjects with severe haemophilia A. (qmul.ac.uk)
  • Consequently, in this study, we delivered intratumorally via protein transfer four molecules, including the chemotactic molecules secondary lymphoid tissue chemokine and Fas ligand and the costimulatory molecules 4-1BBL and TNF-related activation-induced cytokine. (jimmunol.org)
  • Mechanisms of recombinant adenovirus-mediated SD-HA fusion protein proliferation inhibition and induced apoptosis of K562 cells]. (antibody-antibodies.com)
  • The binding of MLB+GFP fusion proteins to the membrane glycans of human cancer cells was also confirmed through the treatment of human metastatic melanoma cells with the fusion protein and the fusions were detected to actively attach and cross the cell membrane. (salford.ac.uk)
  • Finally, the Lectin-Toxin fusions were screened against a range of cancer cell lines specifically WM-115 and WM 266-4 melanoma lines and a healthy melanocyte cell line (HEMn) in order to confirm the fusions binding and crossing the cell membrane of the tissues and also confirm their apoptotic effect. (salford.ac.uk)
  • LAX1 is a single-pass type III membrane protein. (antibody-antibodies.com)
  • in turn, this membrane-anchored palmitated protein A (PPA) serves as a "trap" for secondarily added Fcγ1-derivatized immunostimulatory proteins ( 11 ). (jimmunol.org)
  • ANV-KPI fusion proteins represent a new class of anticoagulants that specifically target the anionic membrane-associated coagulation enzyme complexes present at sites of thrombogenesis and are potentially useful as antithrombotic agents. (bloodjournal.org)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (bio-techne.com)
  • 12. The fusion DNA construct of claim 1, wherein the desired protein is a therapeutic protein. (patentsencyclopedia.com)
  • 1986) Biologic properties in vitro of a recombinant human granulocyte-macrophage colony-stimulating factor. (springer.com)
  • Fluorescent protein-based methodologies have revolutionized the field of virology, allowing us to visualize the location and activity of pseudorabies virus (PRV) particles in vitro (G. A. Smith & Enquist, 2002). (princeton.edu)
  • The recombinant enzyme, both as a fusion protein and as purified full-length hPAH, was phosphorylated in vitro by the catalytic subunit of cyclic AMP-dependent protein kinase. (biochemj.org)
  • The resulting ANV-KPI fusion proteins were 6- to 86-fold more active than recombinant tissue factor pathway inhibitor and tick anticoagulant protein in an in vitro tissue factor-initiated clotting assay. (bloodjournal.org)
  • In turn, the incorporation of the HSP70/EIII chimeric protein in the TI-complex resulted in a twofold increase in its immunogenicity. (mdpi.com)
  • Using HSP70 in the content of the chimeric protein represents an efficient means for presenting the main antigenic domain of the TBEV envelope protein to the immune system, whereas the incorporation of this chimeric protein into the TI-complex further contributes to the development of a stronger immune response against the TBEV infection. (mdpi.com)
  • One of these molecules is the heat shock proteins (HSPs), a group conserved molecular chaperones that are highly effective in inducing immune responses and, therefore, can have an effect on the outcome of adaptive immune responses. (biopharminternational.com)
  • Structurally, the product is a dimeric glycoprotein with a protein molecular weight of 96.9 kilo Daltons (kDa). (creativebiomart.net)
  • Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. (abcam.com)
  • FACTOR IX Fc FUSION PROTEIN (fak tir nine Fc FYOO zhuh n PROH teen) is used in patients with hemophilia B to help prevent and control bleeding. (nationwidechildrens.org)
  • A recombinant factor IX Fc fusion protein (rFIXFc) with a prolonged half-life was developed to reduce the frequency of injections required. (elsevier.com)
  • Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. (harvard.edu)
  • The recombinant protein, R32Leu-Arg, is composed of 32 tetrapeptide repeat sequences from the circumsporozoite protein (R32) linked to the dipeptide, Leu-Arg. (nih.gov)
  • The fusion protein also exhibited a moderate capability for neutralization of TcdA cytotoxicity in both C-PGC2 and CHO-K1 cells, the former with and the latter without cell surface Galα1,3Galβ1,4GlcNAc sequences. (asm.org)
  • Finally, using an insulin tolerance test in mice and various biophysical methods, the structure and function of purified human recombinant insulin was compared with authentic insulin, to verify folding of insulin to its native state. (jascoinc.com)
  • Fragment containing α 1 1.2-subunit (745-892) was subcloned in pGBKT7 vector (Clontech Laboratories, Palo Alto, CA) as a Myc-tagged protein. (diabetesjournals.org)