Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Stable iodine atoms that have the same atomic number as the element iodine, but differ in atomic weight. I-127 is the only naturally occurring stable iodine isotope.
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
A series of steps taken in order to conduct research.
The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
17,21-Dihydroxypregn-4-ene-3,20-dione. A 17-hydroxycorticosteroid with glucocorticoid and anti-inflammatory activities.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
Hypersecretion of THYROID HORMONES from the THYROID GLAND. Elevated levels of thyroid hormones increase BASAL METABOLIC RATE.
Blood proteins that bind to THYROID HORMONES such as THYROXINE and transport them throughout the circulatory system.
A syndrome that results from abnormally low secretion of THYROID HORMONES from the THYROID GLAND, leading to a decrease in BASAL METABOLIC RATE. In its most severe form, there is accumulation of MUCOPOLYSACCHARIDES in the SKIN and EDEMA, known as MYXEDEMA.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Luteinizing hormone regulates steroid production by the interstitial cells of the TESTIS and the OVARY. The preovulatory LUTEINIZING HORMONE surge in females induces OVULATION, and subsequent LUTEINIZATION of the follicle. LUTEINIZING HORMONE consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH and FSH), but the beta subunit is unique and confers its biological specificity.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).
Sites on an antigen that interact with specific antibodies.
An amorphous form of carbon prepared from the incomplete combustion of animal or vegetable matter, e.g., wood. The activated form of charcoal is used in the treatment of poisoning. (Grant & Hackh's Chemical Dictionary, 5th ed)
A hydroxylated metabolite of ESTRADIOL or ESTRONE that has a hydroxyl group at C3, 16-alpha, and 17-beta position. Estriol is a major urinary estrogen. During PREGNANCY, a large amount of estriol is produced by the PLACENTA. Isomers with inversion of the hydroxyl group or groups are called epiestriol.
A genus of PICORNAVIRIDAE causing infectious hepatitis naturally in humans and experimentally in other primates. It is transmitted through fecal contamination of food or water. HEPATITIS A VIRUS is the type species.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The physiologically active and stable hydrolysis product of EPOPROSTENOL. Found in nearly all mammalian tissue.
Antibodies produced by a single clone of cells.
One of the three major groups of endogenous opioid peptides. They are large peptides derived from the PRO-OPIOMELANOCORTIN precursor. The known members of this group are alpha-, beta-, and gamma-endorphin. The term endorphin is also sometimes used to refer to all opioid peptides, but the narrower sense is used here; OPIOID PEPTIDES is used for the broader group.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
A family of gastrointestinal peptide hormones that excite the secretion of GASTRIC JUICE. They may also occur in the central nervous system where they are presumed to be neurotransmitters.
A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.
A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.
A small, unpaired gland situated in the SELLA TURCICA. It is connected to the HYPOTHALAMUS by a short stalk which is called the INFUNDIBULUM.
(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.
A 31-amino acid peptide that is the C-terminal fragment of BETA-LIPOTROPIN. It acts on OPIOID RECEPTORS and is an analgesic. Its first four amino acids at the N-terminal are identical to the tetrapeptide sequence of METHIONINE ENKEPHALIN and LEUCINE ENKEPHALIN.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.
A group of hydroxycorticosteroids bearing a hydroxy group at the 17-position. Urinary excretion of these compounds is used as an index of adrenal function. They are used systemically in the free alcohol form, but with esterification of the hydroxy groups, topical effectiveness is increased.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
A polypeptide hormone of approximately 25 kDa that is produced by the SYNCYTIOTROPHOBLASTS of the PLACENTA, also known as chorionic somatomammotropin. It has both GROWTH HORMONE and PROLACTIN activities on growth, lactation, and luteal steroid production. In women, placental lactogen secretion begins soon after implantation and increases to 1 g or more a day in late pregnancy. Placental lactogen is also an insulin antagonist.
An insoluble support for an ANTIGEN or ANTIBODIES that is used in AFFINITY CHROMATOGRAPHY to adsorb the homologous antibody or antigen from a mixture. Many different substances are used, among them SEPHAROSE; GLUTARALDEHYDE; copolymers of ANHYDRIDES; polyacrylamides, etc.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.
Elements of limited time intervals, contributing to particular results or situations.
The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.
(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
A lactogenic hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). It is a polypeptide of approximately 23 kD. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin receptors are present in the mammary gland, hypothalamus, liver, ovary, testis, and prostate.
A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.
A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN).
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Method of analyzing chemicals using automation.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The periodic shedding of the ENDOMETRIUM and associated menstrual bleeding in the MENSTRUAL CYCLE of humans and primates. Menstruation is due to the decline in circulating PROGESTERONE, and occurs at the late LUTEAL PHASE when LUTEOLYSIS of the CORPUS LUTEUM takes place.
The sum of the weight of all the atoms in a molecule.
Serum proteins with an electrophoretic mobility that falls between ALPHA-GLOBULINS and GAMMA-GLOBULINS.
A polypeptide that is secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Growth hormone, also known as somatotropin, stimulates mitosis, cell differentiation and cell growth. Species-specific growth hormones have been synthesized.
A quinazoline derivative with hypnotic and sedative properties. It has been withdrawn from the market in many countries because of problems with abuse. (From Martindale, The Extra Pharmacopoeia, 30th ed, p604)
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Follicle-stimulating hormone stimulates GAMETOGENESIS and the supporting cells such as the ovarian GRANULOSA CELLS, the testicular SERTOLI CELLS, and LEYDIG CELLS. FSH consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity.
A prostaglandin that is a powerful vasodilator and inhibits platelet aggregation. It is biosynthesized enzymatically from PROSTAGLANDIN ENDOPEROXIDES in human vascular tissue. The sodium salt has been also used to treat primary pulmonary hypertension (HYPERTENSION, PULMONARY).
An anterior pituitary hormone that stimulates the ADRENAL CORTEX and its production of CORTICOSTEROIDS. ACTH is a 39-amino acid polypeptide of which the N-terminal 24-amino acid segment is identical in all species and contains the adrenocorticotrophic activity. Upon further tissue-specific processing, ACTH can yield ALPHA-MSH and corticotrophin-like intermediate lobe peptide (CLIP).
A group of closely related cyclic undecapeptides from the fungi Trichoderma polysporum and Cylindocarpon lucidum. They have some antineoplastic and antifungal action and significant immunosuppressive effects. Cyclosporins have been proposed as adjuvants in tissue and organ transplantation to suppress graft rejection.
INFLAMMATION of the LIVER in humans caused by a member of the HEPATOVIRUS genus, HUMAN HEPATITIS A VIRUS. It can be transmitted through fecal contamination of food or water.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
An aromatized C18 steroid with a 3-hydroxyl group and a 17-ketone, a major mammalian estrogen. It is converted from ANDROSTENEDIONE directly, or from TESTOSTERONE via ESTRADIOL. In humans, it is produced primarily by the cyclic ovaries, PLACENTA, and the ADIPOSE TISSUE of men and postmenopausal women.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
A peptide, of about 33 amino acids, secreted by the upper INTESTINAL MUCOSA and also found in the central nervous system. It causes gallbladder contraction, release of pancreatic exocrine (or digestive) enzymes, and affects other gastrointestinal functions. Cholecystokinin may be the mediator of satiety.
Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status.
A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Pathological processes involving the THYROID GLAND.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
A nonapeptide hormone released from the neurohypophysis (PITUITARY GLAND, POSTERIOR). It differs from VASOPRESSIN by two amino acids at residues 3 and 8. Oxytocin acts on SMOOTH MUSCLE CELLS, such as causing UTERINE CONTRACTIONS and MILK EJECTION.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A potent natriuretic and vasodilatory peptide or mixture of different-sized low molecular weight PEPTIDES derived from a common precursor and secreted mainly by the HEART ATRIUM. All these peptides share a sequence of about 20 AMINO ACIDS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.
The period in the ESTROUS CYCLE associated with maximum sexual receptivity and fertility in non-primate female mammals.
A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A barbiturate that is used as a sedative. Secobarbital is reported to have no anti-anxiety activity.
Syndromes resulting from inappropriate production of HORMONES or hormone-like materials by NEOPLASMS in non-endocrine tissues or not by the usual ENDOCRINE GLANDS. Such hormone outputs are called ectopic hormone (HORMONES, ECTOPIC) secretion.
A metabolite of AMITRIPTYLINE that is also used as an antidepressive agent. Nortriptyline is used in major depression, dysthymia, and atypical depressions.
HORMONES secreted by the gastrointestinal mucosa that affect the timing or the quality of secretion of digestive enzymes, and regulate the motor activity of the digestive system organs.
A 14-amino acid peptide named for its ability to inhibit pituitary GROWTH HORMONE release, also called somatotropin release-inhibiting factor. It is expressed in the central and peripheral nervous systems, the gut, and other organs. SRIF can also inhibit the release of THYROID-STIMULATING HORMONE; PROLACTIN; INSULIN; and GLUCAGON besides acting as a neurotransmitter and neuromodulator. In a number of species including humans, there is an additional form of somatostatin, SRIF-28 with a 14-amino acid extension at the N-terminal.
A group of LEUKOTRIENES; (LTC4; LTD4; and LTE4) that is the major mediator of BRONCHOCONSTRICTION; HYPERSENSITIVITY; and other allergic reactions. Earlier studies described a "slow-reacting substance of ANAPHYLAXIS" released from lung by cobra venom or after anaphylactic shock. The relationship between SRS-A leukotrienes was established by UV which showed the presence of the conjugated triene. (From Merck Index, 11th ed)
A tripeptide that stimulates the release of THYROTROPIN and PROLACTIN. It is synthesized by the neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, TRH (was called TRF) stimulates the release of TSH and PRL from the ANTERIOR PITUITARY GLAND.
Form of radioimmunoassay in which excess specific labeled antibody is added directly to the test antigen being measured.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
A 90-amino acid peptide derived from post-translational processing of pro-opiomelanocortin (POMC) in the PITUITARY GLAND and the HYPOTHALAMUS. It is the C-terminal fragment of POMC with lipid-mobilizing activities, such as LIPOLYSIS and steroidogenesis. Depending on the species and the tissue sites, beta-LPH may be further processed to yield active peptides including GAMMA-LIPOTROPIN; BETA-MSH; and ENDORPHINS.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
The rate dynamics in chemical or physical systems.
Ventral part of the DIENCEPHALON extending from the region of the OPTIC CHIASM to the caudal border of the MAMMILLARY BODIES and forming the inferior and lateral walls of the THIRD VENTRICLE.
Metabolites or derivatives of PROGESTERONE with hydroxyl group substitution at various sites.
A serpin family member that binds to and transports GLUCOCORTICOIDS in the BLOOD.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.

Autoantibodies to gastrin in patients with pernicious anaemia--a novel antibody. (1/8213)

Autoantibodies arise when there is a breakdown in immunological tolerance. Autoantibodies to parietal cells and intrinsic factor are found in autoimmune atrophic gastritis (AAG) and are associated with elevated plasma gastrin. Endogenous gastrin autoantibodies have not been described to date. The aim of this study was to investigate the occurrence of autoantibodies to gastrin. Plasma from 50,000 patients, including more than 2000 with AAG, was tested. Gastrin was measured by radioimmunoassay (RIA) in whole plasma and the presence of autoantibody determined by using a control which omitted assay antibody. The quantity and affinity of gastrin autoantibodies was assessed. Three patients had autoantibodies to gastrin. All three had AAG and pernicious anaemia (PA). The antibodies were of low titre and relatively high affinity. Free circulating plasma gastrin levels were within the normal range, but total gastrin levels were elevated. This is the first description of autoantibodies to endogenous gastrin. The incidence of antibodies to gastrin is low, they are found in association with PA, and they may lead to falsely low measurements of plasma gastrin.  (+info)

Immunoglobulin-specific radioimmunoprecipitation assays for quantitation of nasal secretory antibodies to hemagglutinin of type A influenza viruses. (2/8213)

Radioimmunoprecipitation (RIP) assays were developed to selectively quantitate class-specific antibodies to purified hemagglutinins (HA) of type A influenza virus in nasal secretions. Rabbit anti-human secretory piece of immunoglobulin A (IgA) and rabbit anti-human IgG were used as second antibodies. A third antibody, goat anti-rabbit IgG, was incorporated into the system to separate immune complexes formed between iodinated HA, nasal wash test specimen, and second antibody. The utilization of this reagent avoided the need for large quantities of IgA and IgG antibody-negative carrier secretions. Nasal was specimens obtained from 14 adults immunized with an inactivated type A influenza virus vaccine were evaluated by RIP and viral neutralization assays. Significant homologous postvaccination secretory IgA and IgG antibody levels were demonstrable in 13 (93%) of individuals by RIP, whereas only 5 (36%) exhibited rises by viral neutralization tests. Moreover, the geometric mean IgA and IgG antibody levels were at least 20- and 37-fold greater than the neutralizing antibody titer. The pattern of heterologous immunoglobulin-specific antibody responses tended to be similar to those observed with the homologous HA subunit.  (+info)

Influences of Helicobacter pylori on serum pepsinogen concentrations in dialysis patients. (3/8213)

BACKGROUND: Patients with impaired renal function have been known to have elevated concentrations of serum pepsinogens, which are raised by Helicobacter pylori infection of the stomach. The present study was performed to examine the effect of H. pylori infection on serum pepsinogen concentrations in dialysis patients. METHODS: Forty nine patients on dialysis and 48 subjects with no known kidney disease were examined for upper gastroduodenal endoscopy, H. pylori infection and serum concentrations of pepsinogen I and II. The status of H. pylori infection was evaluated from results of a urease test, histology and culture of biopsy specimens of the gastric mucosa. Serum pepsinogen levels were measured by radioimmunoassay. RESULTS: Serum concentrations of pepsinogen I and II were elevated in the dialysis patients in comparison with those in the controls (277.4+/-24.2 vs 52.6+/-4.0 pg/ml, P<0.01 for pepsinogen I, and 30.2+/-2.9 vs 14.9+/-1.3 pg/ml, P<0.01 for pepsinogen II). In both the dialysis patients and controls, those with H. pylori infection had significantly higher concentrations of serum pepsinogen I and II and a lower ratio of pepsinogen I to pepsinogen II than those without infection. Among the controls, 15 of 25 subjects with atrophic gastritis had a pepsinogen I/pepsinogen II ratio < or = 3.0, while only two out of 17 patients on dialysis fell into this range. CONCLUSIONS: We conclude that H. pylori status should be taken into account when serum pepsinogen concentrations are evaluated in dialysis patients.  (+info)

Pregnancy detection and the effects of age, body weight, and previous reproductive performance on pregnancy status and weaning rates of farmed fallow deer (Dama dama). (4/8213)

Fallow does (n = 502) of different ages (mature, 2-yr-old, and yearling) were maintained with bucks for a 60-d breeding season to determine whether previous reproductive performance and changes in BW affect doe pregnancy rates and to compare the effectiveness of ultrasonography and serum pregnancy-specific protein B (PSPB) for the detection of pregnancy in fallow does. Ultrasonography was performed, blood samples collected, and BW recorded at buck removal (d 0) and at 30 and 90 d after buck removal. Lactational status (lactating = WET; nonlactating = DRY) were determined from farm records taken at weaning prior to each breeding season (autumn 1990 through autumn 1994). Ultrasonography and PSPB for determining pregnancy were in agreement 93% of the time. Overall pregnancy rates did not differ (P>.10) relative to age of the doe; the combined pregnancy rate was 92%. We also determined that 82.9% of does conceived early in the breeding season and that the incidence of embryonal-fetal mortality during the first 90 d after buck removal was 2.8%. In general, mature and 2-yr-old DRY does were heavier and had lower pregnancy rates than WET does. The overall weaning rate for all does was 77.9%. Loss in the number of fawns from pregnancy detection to weaning was equivalent to 14.8% for mature does, 24.7% for 2 yr old does, and 42.5% for yearling does. These data indicate that even though pregnancy rates were relatively high, further study is needed to determine the causes associated with subsequent fawn losses, particularly among yearling does. As a production tool, lactational WET/ DRY status testing was found to be an acceptable means for determining the reproductive potential of individual does within the herd. In addition, serum PSPB may be used in place of ultrasonography for pregnancy diagnosis in fallow deer as early as d 30 after buck removal.  (+info)

EGF precursor mRNA and membrane-associated EGF precursor protein in rat exorbital lacrimal gland. (5/8213)

This study was designed to demonstrate the presence of epidermal growth factor (EGF) in the rat exorbital lacrimal gland. EGF precursor gene transcription was demonstrated first by RT-PCR analysis of lacrimal gland RNA using a set of specific primers and second by Northern blot analysis of rat lacrimal gland mRNA. A rabbit polyclonal antibody (rEGF2) directed against rat submaxillary gland EGF was used to detect EGF-containing proteins by RIA. Results indicate that the rat lacrimal gland does not contain detectable soluble and mature EGF but that the EGF immunoreactivity is associated with the membrane-enriched fraction. Analysis of the detergent-solubilized membrane proteins by gel filtration shows that membrane-associated EGF immunoreactivity was present as a high-molecular-mass protein. Moreover, as shown by Western blot analysis, a specific anti-rat EGF precursor antibody (ppEGF1) can immunoprecipitate a 152-kDa EGF-containing protein. Taken together, these results demonstrate for the first time both EGF precursor gene transcription and EGF precursor protein expression in a lacrimal tissue, i.e., the rat exorbital lacrimal gland. The demonstration that EGF appears to be stored only as its full-length membrane precursor may provide important information to study the regulation of its secretory process.  (+info)

Cardiovascular, endocrine, and renal effects of urodilatin in normal humans. (6/8213)

Effects of urodilatin (5, 10, 20, and 40 ng. kg-1. min-1) infused over 2 h on separate study days were studied in eight normal subjects with use of a randomized, double-blind protocol. All doses decreased renal plasma flow (hippurate clearance, 13-37%) and increased fractional Li+ clearance (7-22%) and urinary Na+ excretion (by 30, 76, 136, and 99% at 5, 10, 20, and 40 ng. kg-1. min-1, respectively). Glomerular filtration rate did not increase significantly with any dose. The two lowest doses decreased cardiac output (7 and 16%) and stroke volume (10 and 20%) without changing mean arterial blood pressure and heart rate. The two highest doses elicited larger decreases in stroke volume (17 and 21%) but also decreased blood pressure (6 and 14%) and increased heart rate (15 and 38%), such that cardiac output remained unchanged. Hematocrit and plasma protein concentration increased with the three highest doses. The renin-angiotensin-aldosterone system was inhibited by the three lowest doses but activated by the hypotensive dose of 40 ng. kg-1. min-1. Plasma vasopressin increased by factors of up to 5 during infusion of the three highest doses. Atrial natriuretic peptide immunoreactivity (including urodilatin) and plasma cGMP increased dose dependently. The urinary excretion rate of albumin was elevated up to 15-fold (37 +/- 17 micrograms/min). Use of a newly developed assay revealed that baseline urinary urodilatin excretion rate was low (<10 pg/min) and that fractional excretion of urodilatin remained below 0.1%. The results indicate that even moderately natriuretic doses of urodilatin exert protracted effects on systemic hemodynamic, endocrine, and renal functions, including decreases in cardiac output and renal blood flow, without changes in arterial pressure or glomerular filtration rate, and that filtered urodilatin is almost completely removed by the renal tubules.  (+info)

cGMP-dependent and -independent inhibition of a K+ conductance by natriuretic peptides: molecular and functional studies in human proximal tubule cells. (7/8213)

In immortalized human kidney epithelial (IHKE-1) cells derived from proximal tubules, two natriuretic peptide receptors (NPR) were identified. In addition to NPR-A, which is bound by atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and urodilatin (URO), a novel form of NPR-B that might be bound by C-type natriuretic peptide (CNP) was identified using PCR. This novel splice variant of NPR-B (NPR-Bi) was also found in human kidney. Whereas ANP, BNP, and URO increased intracellular cGMP levels in IHKE-1 cells in a concentration-dependent manner, CNP had no effect on cGMP levels. To determine the physiologic responses to these agonists in IHKE-1 cells, the membrane voltage (Vm) was monitored using the slow whole-cell patch-clamp technique. ANP (10 nM), BNP (10 nM), and URO (16 nM) depolarized these cells by 3 to 4 mV (n = 47, 7, and 16, respectively), an effect that could be mimicked by 0.1 mM 8-Br-cGMP (n = 15). The effects of ANP and 8-Br-cGMP were not additive (n = 4). CNP (10 nM) also depolarized these cells, by 3+/-1 mV (n = 28), despite the absence of an increase in cellular cGMP levels, indicating a cGMP-independent mechanism. In the presence of CNP, 8-Br-cGMP further depolarized Vm significantly, by 1.6+/-0.3 mV (n = 5). The depolarizations by ANP were completely abolished in the presence of Ba2+ (1 mM, n = 4) and thus can be related to inhibition of a K+ conductance in the luminal membrane of IHKE-1 cells. The depolarizations attributable to CNP were completely blocked when genistein (10 microM, n = 6), an inhibitor of tyrosine kinases, was present. These findings indicate that natriuretic peptides regulate electrogenic transport processes via cGMP-dependent and -independent pathways that influence the Vm of IHKE-1 cells.  (+info)

Mediation of humoral catecholamine secretion by the renin-angiotensin system in hypotensive rainbow trout (Oncorhynchus mykiss). (8/8213)

The individual contributions of, and potential interactions between, the renin-angiotensin system (RAS) and the humoral adrenergic stress response to blood pressure regulation were examined in rainbow trout. Intravenous injection of the smooth muscle relaxant, papaverine (10 mg/kg), elicited a transient decrease in dorsal aortic blood pressure (PDA) and systemic vascular resistance (RS), and significant increases in plasma angiotensin II (Ang II) and catecholamine concentrations. Blockade of alpha-adrenoceptors before papaverine treatment prevented PDA and RS recovery, had no effect on the increase in plasma catecholamines, and resulted in greater plasma Ang II concentrations. Administration of the angiotensin-converting enzyme inhibitor, lisinopril (10(-4) mol/kg), before papaverine treatment attenuated the increases in the plasma concentrations of Ang II, adrenaline, and noradrenaline by 90, 79, and 40%, respectively and also prevented PDA and RS recovery. By itself, lisinopril treatment caused a gradual and sustained decrease in PDA and RS, and reductions in basal plasma Ang II and adrenaline concentrations. Bolus injection of a catecholamine cocktail (4 nmol/kg noradrenaline plus 40 nmol/kg adrenaline) in the lisinopril+papaverine-treated trout, to supplement their circulating catecholamine concentrations and mimic those observed in fish treated only with papaverine, resulted in a temporary recovery in PDA and RS. These results indicate that the RAS and the acute humoral adrenergic response are both recruited during an acute hypotensive stress, and have important roles in the compensatory response to hypotension in rainbow trout. However, whereas the contribution of the RAS to PDA recovery is largely indirect and relies on an Ang II-mediated secretion of catecholamines, the contribution from the adrenergic system is direct and relies at least in part on plasma catecholamines.  (+info)

TY - JOUR. T1 - Human prostatic acid phosphatases. II. A double-antibody radioimmunoassay. AU - Choe, B. K.. AU - Pontes, E. J.. AU - Morrison, M. K.. AU - Rose, N. R.. PY - 1978. Y1 - 1978. N2 - A double-antibody radioimmunoassay method for prostate-specific acid phosphatase (PAP) is presented. Experimental details are outlined to assess the reproducibility and reliability of the method under various assay conditions. The upper limit of the serum PAP levels in the present assay was set at 2.4 ng/100 μl by 162 determinations of normal serum samples. The serum PAP levels of patients with nonprostatic malignant tumors fell in the normal range, whereas the levels higher than 4.0 ng/100 μl were found in patients with prostatic carcinoma.. AB - A double-antibody radioimmunoassay method for prostate-specific acid phosphatase (PAP) is presented. Experimental details are outlined to assess the reproducibility and reliability of the method under various assay conditions. The upper limit of the serum ...
Adenosine was coupled to human serum albumin by two different procedures that preserved the purine and ribose rings. The conjugates were evaluated for antigenicity in rabbits and guinea pigs. A conjugate containing 2′(3′)-O-succinoyladenosine failed to elicit antibodies, whereas one containing laevulinic acid (O2′, 3′-adenosine-acetal) elicited antibodies in all animals injected. The affinity and specificity of binding of adenosine to three selected antisera were evaluated. Dissociation constants of 31-187 nM were observed. Displacement of adenosine binding to all antisera by adenine 5′-nucleotides, adenine, inosine and hypoxanthine required more than 1000-fold higher concentrations than of adenosine itself. Similar affinities for adenosine and 2′-deoxyadenosine were observed. By exploiting the high specificity of the antisera, a radioimmunoassay method was established that was capable of detecting down to 1 pmol of adenosine (20 nM) in unfractionated heart perfusates and cell ...
Law, Brian (1983) RADIOIMMUNOASSAY METHODS FOR THE FORENSIC ANALYSIS OF CANNABINOIDS IN BIOLOGICAL FLUIDS Doctoral thesis, University of Surrey. ...
Current immunoassays for interleukin-1 beta (IL-1 beta) are effective for analyzing fluids derived from cultured cells. However, IL-1 beta determinations in human plasma or serum samples are technically complicated by higher protein and lipid concentrations, physicochemical differences which exist between samples from healthy subjects and those experiencing acute phase responses, and by the fact that IL-1 beta can be produced and degraded in the blood collection tube after the sample is drawn. A simple chloroform extraction process has been developed which eliminates several of the interfering factors from plasma samples and increases the amount of IL-1 beta detected by radioimmunoassay and lymphocyte activation assay. In the radioimmunoassay, rabbit sera was found to influence the accuracy and variability of plasma measurements. Improvements in radioimmunoassay reagents and methods are reported which reduce this influence. Finally, different concentrations of IL-1 beta were measured depending on
This paper describes a rapid sensitive solid phase radioimmunoassay procedure for staphylococcal enterotoxin B, which can be applied to A and C. The procedure is 100-fold more sensitive thandescribed immunodiffusion and precipitin methods and is reliable at a level of 0.01 µg/ml. It is usable for detection ad measurement of enterotoxin in foodstuffs, biologic fluids and toxin preparations, both purified and crude.. ...
Background: PYY is a gut hormone that physiologically inhibits appetite. Currently only radioimmunoassay (RIA) techniques are available for its estimation. The aim of the study was to evaluate our in-house assay for precision, detection limit, interference of haemolysis, suitability of serum versus plasma and biological variation within one subject.. Method: All samples were obtained from one subject on two occasions following an over night fast and the consumption of 1000 and 3000 kcal meals on the respective days. Plasma was aliquoted in multiple samples. Coefficient of variation (CV) was measured at three concentrations (13.4, 16.4, 31.7pmol/L). The influence of haemolysis was evaluated by diluting severely haemolysed blood with non haemolysed blood obtained at the same venepuncture. All plasma samples were obtained in lithium heparin and trasylol tubes. Serum samples were obtained in a tube with trasylol and a clot activator. Carry over and the detection limit of the assay were also ...
The aim of the current study was to describe the use of a pool of different antisera raised against pregnancy-associated glycoproteins (PAGs; purified from both ovine and caprine placentas) for early pregnancy diagnosis in ovine species. Sixty-three
Polyspecific antiserum against total rat serum proteins was used to develop specific and sensitive radioimmunoassays for fetuin and bikunin, two minor protein components of rat plasma. The radioimmunoassays proved to be highly useful to trace bikunin and fetuin in the course of developing isolation procedures, since neither specific functional assays nor monospecific antisera were available. The two examples demonstrate that, in general, it will be possible to develop a specific and sensitive radioimmunoassay with antiserum raised against a crude antigen preparation, such as a body fluid or a tissue extract, provided that a minute amount of pure antigen is available for preparing the radioiodinated antigen. ...
E. Piall, N. Curtin, W. Aherne, A. Harris, V. Marks; The Quantification of 2′-Deoxyuridine 5′-Triphosphate in Anti-Folate Treated Cells using a Sensitive Radioimmunoassay. Clin Sci (Lond) 1 January 1987; 73 (s17): 18P. doi: https://doi.org/10.1042/cs073018Pa. Download citation file:. ...
TY - JOUR. T1 - Detection of interleukin 1α and 1β in rabbit tissues during endotoxemia using sensitive radioimmunoassays. AU - Clark, B. D.. AU - Bedrosian, I.. AU - Schindler, R.. AU - Cominelli, F.. AU - Cannon, Joseph Gerard. AU - Shaw, A. R.. AU - Dinarello, C. A.. PY - 1991/12/1. Y1 - 1991/12/1. N2 - Interleukin 1 (IL-1) is a primary mediator of a wide variety of immunologic and inflammatory responses, including reactions to microbial infections. To study this cytokine in an animal model, we have developed specific and sensitive radioimmunoassays for the quantitation of rabbit IL- 1α and IL-1β. The sensitivity (limit of detection at 95% confidence level) of our assay for IL-1α and 1β was 20-40 and 40-80 pg/ml, respectively. Recovery of IL-1 from tissues ranged from 75 to 107%, with a mean of 95% for IL-1α and 89% (range 19-98) for IL-1β. We employed these assays in in vivo and in vitro studies. In an in vivo model, we measured the amount of rabbit IL-1α and 1β protein present in ...
The involvement of central catecholaminergic neurons in tonic gonadotrophin release was investigated. Male rats were given a single cerebral intraventricular injection of 6-hydroxydopamine hydrochloride (6-OHDA; 170 μg free base) dissolved in 0·001 n-HCL. Plasma LH and FSH were measured by double antibody radioimmunoassay. In the 6-OHDA-treated animals, LH was significantly reduced (P,0·001 after 1 hr and remained consistently lower for 8 hr. After a transient elevation, the controls showed no significant change (P,0·01). No difference in LH concentrations was found between experimental groups sampled at 2 days or later (P,0·01). Levels of FSH were less consistently different. Administration of 6-OHDA dissolved in different vehicles, with varied pH and osmolarity, gave similar results which suggests that the quinone derivative of 6-OHDA is effective in producing the catecholaminergic impairment. These findings indicate that LH release in the male rat may be controlled (or at least ...
The pneumococci possess, in addition to type-specific capsular polysaccharides, a number of antigens common to the species. Antibodies to phosphocholine (PC), a major determinant of C-carbohydrate, have been shown to protect mice from experimental pneumococcal infection, but little is known of the role of anti-PC antibodies in humans or the extent to which anti-PC levels are affected by carriage or infection. We examined 115 sera from 30 infants, who were followed propsectively from birth through 4 years of age, for the presence of immunoglobulin M antibody to PC, using a solid-phase radioimmunoassay method. Infants were found to develop antibody to PC in response to pneumococcal carriage and infection, and nearly all infants developed some antibody. Antibody levels increased with age. By using a regression model including both age and nasopharyngeal carriage of pneumococci, anti-PC levels were found to be highest after exposure to two or three different types of pneumococci; levels were highest ...
TY - JOUR. T1 - Central obesity and hypertension: the role of plasma endothelin. Am J Hypertens. AU - Licata, Giuseppe. AU - Corrao, Salvatore. AU - Scaglione, Rosario. AU - Parrinello, Gaspare. AU - Licata, Anna. AU - Pinto, Antonio. AU - Parrinello, Gaspare. PY - 1996. Y1 - 1996. N2 - Hypertension and central obesity are two conditions closely linked, but the mechanisms responsible for obesity-associated hypertension are still unclear. In the last few years, several studies addressed the role of endothelin-1 (ET-1) in the development and maintenance of hypertension. This study was designed to evaluate plasma ET-1 in normotensive and hypertensive central obese subjects compared with a lean healthy group. Our final goal was to analyze the relationship between plasma ET-1, blood pressure, and left ventricular structure and function in central obese subjects (both normotensives and hypertensives). ET-levels have been assessed by the radioimmunoassay method in 20 lean normotensives and in 57 ...
Summary. Baboon CG from the urine and placenta (Days 33-45 of gestation) was purified by ammonium acetate/alcohol extraction followed by ionic exchange, gel filtration and affinity chromatography. The CG activity was monitored using a double-antibody radioimmunoassay utilizing anti-baboon CG and hCG both as the labelled ligand and standard. Biological activity was measured by the rat luteal cell radio-receptor assay. The purified preparation exhibited heterogeneity in terms of its behaviour during ionic exchange chromatography and isoelectric focussing. Like hCG, baboon CG was made up of two non-covalently linked subunits: the Stokes radii were 36·5, 29·0 and 22·0 × 10−10 m for native CG, the beta subunit and the alpha subunit. The material focussed between pH 4·2 and 5·5. Relative to the second international hCG standard the biological potency of the purified urinary baboon CG was 4058 i.u. whilst the immunological activity was 4364 i.u. It is concluded that baboon and human CG are ...
The Medical Journal of the Islamic Republic of Iran (MJIRI), is a quarterly and an editorially independent publication owned by Iran University of Medical Sciences and aims to be a publication of international repute for reporting current regional and international adventures in all aspects of the medicine. MJIRI welcomes contributions in this field in the form of original research articles, case reports, letter to the editor, review articles, brief communications, debate articles, and study protocols.
Two new monoclonal antibodies (Lym-1 and Lym-2), reactive with the cell surface of B-lymphocytes and derived tumors, have been produced using tumor cell nuclei preparations as immunogens. Specificity screens using live cell radioimmunoassay techniques with 52 well-characterized human lymphoma and leukemia cell lines showed that both Lym-1 and Lym-2 bound to cell lines of B-cell lineage but were unreactive with those of T-cell, myeloid, or erythroid derivation. The B-cell specificity of these reagents was confirmed on 36 lymphoma and 15 leukemia biopsy specimens by using immunoperoxidase or immunofluorescence techniques. Additionally, flow cytometric analysis of 22 lymphoma biopsies showed that the majority of B-cell tumors were Lym-1 and/or Lym-2 positive and that within a given biopsy, a high percentage of the malignant cell population was stained. In both the immunoperoxidase and flow cytometric studies, reactive T-cells or T-cell lymphomas were consistently negative with the exception of ...
A solid phase radioimmunoassay for adrenal antibodies is described. In the assay plastic tubes coated with adrenal microsomes (100 micrograms/ml) were incubated with human sera diluted from 1:50 to 1:5000 and the retained antibodies detected by subse
p,Serum specimens from 12 patients with type A hepatitis were analyzed for immunoglobulin M-type antibody to hepatitis A virus (IgM anti-HA). A recently developed solid-phase radioimmunoassay kit for IgM anti-HA (HAVAB-M, Abbott Laboratories) and a competitive binding radioimmunoassay kit (HAVAB, Abbott Laboratories) with or without 2-mercaptoethanol treatment, as modified by Yano et al. (Acta Hepatol. Jpn. 21, 704-712, 1980) were used to obtain an M-index. All specimens obtained within 60 days of the onset of illness and specimens from 2 of 4 patients later than 60 days after the onset were positive with the HAVAB-M test. This test gave negative results to sera which were positive for anti-HA by a standard HAVAB test in the following: 3 patients with type B hepatitis; 5 with non-A, non-B hepatitis; 11 healthy adults; and 10 sera strongly positive for rheumatoid factor. The M-index for type A hepatitis in sera within 30 days of the onset (mean value of the M-index, m, = 1.52; standard deviation, ...
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Direct Radioimmunoassay for Human Renin Substrate and its Measurement in Plasma from Essential Hypertensive Patients, Diabetic Patients and Pregnant Women : THE 13TH CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION (1984 ...
Radioimmunoassay (RIA) ist eine Methode, die die Menge eines Antigens in einer Probe mit hoher Empfindlichkeit messen kann. Prinzipiell kann jede biologisc...
Evidence for a Nonspecific Factor Interfering in the Radioimmunoassay of Somatoliberin-Like Immunoreactivity in Human Seminal ...
Horn, K.; Blümel, K. R.; Koeppen, D. und Scriba, Peter Christian (1974): Radioimmunoassay for T3 in serum: Necessity of prior extraction. 20.Symposion der Deutschen Gesellschaft für Endokrinologie, February 27-March 2, 1974, Tübingen. ...
BioAssay record AID 302415 submitted by ChEMBL: Inhibition of COX2 in LPS-stimulated J774 cells assessed as inhibition of PGE2 levels at 1 uM by radioimmunoassay.
Verstraeten, A A.; Hageman, P C.; and Kwa, H G., A radioimmunoassay for mtv-antigens. (1973). Subject Strain Bibliography 1973. 342 ...
Follow assay procedures as outlined in USP1 or AOAC.2 Use levels of B12 in the preparation of the standard curve according to these references. It is essential that a standard curve be constructed each time an assay is run. Autoclave and incubation conditions can influence the standard curve reading and cannot always be duplicated. Generally satisfactory results are obtained with B12 at the following levels: 0.0, 0.025, 0.05, 0.075, 0.1, 0.125, 0.15, 0.2 and 0.25 ng per assay tube (10 mL ...
By the 1970s, there were already many examples of how radioimmunoassays improved diagnostic laboratory methods. Although mildly disadvantaged because of the need for special conditions related to the...
The Ordinary Buffet Serum Value Size - The Ordinary Buffet Serum is a multi-technology peptide serum.  This formula combines a compre
validation status unknown, seller recommendations provided in 2012: ELISA; Flow Cytometry; Immunohistochemistry; Immunoprecipitation; Radioimmunoassay; Western Blot; ELISA, Flow Cytometry, Immunohistochemistry-Fr, Immunohistochemistry-P, Immunohistochemistry-R, Immunoprecipitation, Radioimmunoassay, Western ...
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According to recent investigations neopterin (a pyrazinopyrimidine derivative) is a biochemical marker that reflects the activity of the proinflammatory immunocellular system of the synovial tissue in rheumatoid arthritis (RA). Interferon gamma, derived from antigen activated T lymphocytes, stimulates macrophages to synthesise and release neopterin into the culture supernatant in vitro. To extend this in vitro model to a clinical level a sensitive new radioimmunoassay technique was used to measure neopterin concentrations in the synovial fluid (SF) of 17 patients with active RA, nine with osteoarthritis, and six with acute gout, and in that of 12 controls undergoing meniscectomy. The SF neopterin concentrations were significantly higher in patients with RA than in the other groups of patients, particularly the controls. Multivariant analysis showed that SF neopterin concentrations correspond better with the systemic inflammatory activity of RA than with the local disease activity of the knee ...
In this paper a method for the radioimmunological determination of triamcinolone acetonide (TAAc) in plasma is described. The antiserum used in the radioimmunological determinations showed high...
Oestrus was synchronised with PGF2-alpha in four pluriparous West African Dwarf (WAD) goats and blood collected through jugular venupuncture. The length of the oestrous cycle and oestrus duration were observed in three successive cycles while peripheral plasma progesterone and oestradiol-17B levels were determined by the radioimmunoassay technique. The mean oestrous cycle lengths and oestrus duration were 19.4 ± 1.7 d and 33.3 ± 6.6 h, respectively. Progesterone levels were very low during the first four days of the cycle (day 1 = day of oestrous) with the lowest level of 0.3 ± 0.02 ng/ml being recorded on day 2 of the cycle. There was a steady rise in the levels during the cycle until day 15 when the highest level of 2.2 ± 0.05 ng/ml was recorded which was followed by a sharp decline on day 20 (P ,0.05). The profile of oestradiol-17B was erratic during the cycle. Mean maximum and minimum concentrations were recorded on day 1 (152.6 ± 31.6 ng/ml) and day 2 (58.8 ± 22.6 ng/ml), ...
Huge collection of Rosalyn S. Yalow quotes. Born on 1921-07-19 Died on 2011-05-30. Nickname: Rosalyn Sussman. Radioimmunoassay, (RIA) Rosalyn Sussman Yalow was an American medical physicist, and a co-winner of the 1977 Nobel Prize in Physiology or Medicine for development of the radioimmunoassay technique. She was the second American woman to be awarded the Nobel Prize in Physiology or Medicine after Gerty Cori.
Abstract: Heparinized blood samples were taken from male immature and mature camels of the Shaalah breed, housed at the University Animal farm, during the rutting and non-rutting period. Other blood samples were also collected from camels slaughtered at defined seasons (summer, autumn, winter and spring) and from the Buraydah slaughter-house. In addition, specimens from the testes were also taken to confirm the difference between the immature and the mature animals during the non-rutting and rutting seasons. The plasma obtained from the collected blood samples was used for estimation of the following hormones, Melatonin (MLT), Follicle Stimulating Hormone (FSH), Leutinizing Hormone (LH), Testosterone and Prolactin (PRL) using the radioimmunoassay technique. Specimens of testes tissue were fixed in calcium formol, processed for histological examination using standard procedures and stained with H&E. The results clearly differentiated the samples as immature and mature during the non-rutting and ...
TY - JOUR. T1 - Bleomycin serum pharmacokinetics as determined by a radioimmunoassay and a microbiologic assay in a patient with compromised renal function. AU - Crooke, Stanley T.. AU - Luft, Friedrich. AU - Broughton, Alan. AU - Strong, James. AU - Casson, Kay. AU - Einhorn, Lawrence. PY - 1977/4. Y1 - 1977/4. N2 - Serum and plasma bleomycin concentrations were determined in a patient with renal dysfunction at two creatinine clearances. The results obtained with a new radioimmunoassay and the microbiologic assay were compared. It was shown: 1) that the clearance of bleomycin from the blood is markedly retarded in severe renal dysfunction, 2) that clearance of bleomycin varies with creatinine clearance, 3) that bleomycin is probably not dialyzable, 4) that determinations on serum and plasma were equivalent, and 5) that the radioimmunoassay and microbiologic assays gave equivalent results (P , 0.001).. AB - Serum and plasma bleomycin concentrations were determined in a patient with renal ...
TY - JOUR. T1 - Endothelium-independent contractions to jvg-monomethyl-l-arginine in canine basilar artery. AU - Katušić, Zvonimir S.. PY - 1991/11. Y1 - 1991/11. N2 - Background and Purpose: NG-substituted analogues of L-arginine are potent and selective inhibitors of nitric oxide synthase(s). The present study was designed to determine the effects of these analogues on the vascular smooth muscle of isolated canine basilar arteries. Methods: Basilar artery rings without endothelium were suspended for isometric tension recording in Krebs-Ringer bicarbonate solution bubbled with 94% O2-6% CO2 (temperature=37°C, pH=7.4). A radioimmunoassay technique was used to determine the levels of guanosine 3,5-cyclic monophosphate (cyclic GMP). Results: NG-Monomethyl-L-arginine (L-NMMA) caused concentration-dependent contractions, whereas the D-enantiomer and NG-nitro-L-arginine did not. Contractions to L-NMMA were reduced in the presence of L-arginine but not in the presence of D-arginine. Superoxide ...
to achieve the student with the theoretical skills that allow an adequate knowledge of physical and biological phenomena at the base of radioimmunoassay techniques.. ...
Figure 2: The Effects of Endogenous Thyroglobulin Antibody on the Measurement of Serum Thyroglobulin using Immunoenzymatic (Sandwich) Method. Thyroglobulin Immunoassays. Several immunoassays are commercially available to measure thyroglobulin including the competitive radioimmunoassay (RIA) and the one-step immunoenzymatic (sandwich) assay (IMA). The RIA method is older and more labor intensive compared to IMA. In the RIA method, thyroglobulin competes with 125I labeled human thyroglobulin for a rabbit antibody against human thyroglobulin. After six days of incubation, thyroglobulin - antibody complexes are precipitated with a second anti-rabbit antibody. This method can detect both free thyroglobulin and thyroglobulin bound to antibody. The radioactivity is inversely proportional to the thyroglobulin concentration (2). In contrast to RIA, the IMA can be performed within one hour. The thyroglobulin in the patients sample is mixed with a biotinylated reagent that contains four monoclonal ...
If indirect immunofluorescence assay (IFA) (ANN1C, ANN2C, ANN3C, PCA1C, PCA2C, PCTRC, AMPHC, CRMC, AGN1C) is indeterminate, then paraneoplastic autoantibody Western blot is performed at an additional charge.. If IFA pattern suggest NMO/AQP4-IgG, then NMO/AQP4-IgG FACS is performed at an additional charge.. If NMO/AQP4-IgG FACS screen assay requires further evaluation, then NMO/AQP4-IgG FACS titration assay is performed at an additional charge.. If IFA patterns suggest CRMP-5-IgG, then CRMP-5-IgG Western blot is performed at an additional charge.. If IFA patterns suggest GAD65 antibody, then GAD65 antibody radioimmunoassay is performed at an additional charge.. If IFA patterns suggest neuronal voltage-gated potassium channel-complex autoantibody, then VGKC-complex antibody IPA is performed at an additional charge.. If VGKCC ,0.00, LGI1-IgG CBA, CSF and CASPR2-IgG CBA, CSF are performed at an additional charge.. If IFA patterns suggest amphiphysin antibody, then amphiphysin Western blot is ...
490 blood samples have been examined for the comparison of the various methods concerned with the detection of the Australia Antigen (Au). The following methods have been comparatively examined: immunodiffusion (ID) counterelectrophoresis (CEP), complement fixation (CF) passive hemoagglutination (HA) and solid phase radioimmunoassay RIA. On the basis of the results so far obtained the RIA appears the most sensitive and specific method, followed by the HA. In order to obtain reproducible results for diagnostic purposes we suggest to carry out routinely two different techniques together (possibly RIA and HA), while for screening purposes just one technique, choosed between the two most indicative ones, shall be employed.
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An immobilized immunoadsorbent for use in immunoassay and radioimmunoassay procedures includes, in a preferred form, a mass of superficially porous refractory particles having a water insoluble polymer, such as dextran, bonded thereto. The polymer in turn has bonded to it, by covalent bonds, antibodies capable of binding a specific antigen thereto and stoichiometrically releasing the bound antigen by the use of a rinsing solution which does not adversely affect the binding of the antibody to the polymer. Preferably, each of the porous particles includes an impervious core which has joined thereto sufficient layers of microparticles to form an outer porous coating on the core. Methods of forming such an immunoadsorbent and for using the same and structure for supporting the same are also disclosed.
The in vivo release of cholecystokinin (CCK)-like material (CCKLM) was measured in the frontal cortex of freely moving rats using the microdialysis technique combined with a sensitive radioimmunoassay. Local perfusion of K+ (100 mM)-enriched artificial CSF resulted in a 10-fold increase in CCKLM outflow, as compared with that occurring under basal resting (K+ = 3.0 mM) conditions, and this effect could be completely prevented by removal of Ca2+ in the perfusing fluid. Chromatographic analyses demonstrated that CCK-8S contributed to 70% of CCKLM. Stressful stimuli such as a 2-min exposure to diethyl ether and a 30-min restraint produced a marked but transient increase in cortical CCKLM release. In addition, anxiety-like behavior induced by the systemic administration of yohimbine (5 mg/kg i.p.) was associated with a long-lasting enhancement in the peptide outflow. Pretreatment with the potent anxiolytic drug diazepam (5 mg/kg i.p., 5 min before each condition), which exerted no effect on its own,
Radioimmunoassay for ovine, caprine and bovine prolactin in plasma and tissue extracts: 1. A radioimmunoassay for ovine prolactin is described based on the inhi
A radioimmunoassay with specificity for the a1 allotype determinant of rabbit immunoglobulin (Ig) heavy chains was used for the quantitation of VH in detergent extracts of lymph node cells from a1a1/b4b4 rabbits. B cell-derived Ig in the same extracts was estimated with a radioimmunoassay specific for L-chain. About 2 X 10(5) molecules/cell was found by both assays when assuming a molecular weight of 1.5 X 10(5) dalton. Extracts prepared from lymphocytes depleted of B cells (surface Ig+ cells) were also found to contain corresponding amounts of a1 and L-chain, with values varying from about 10(3) to 10(4) molecules/cellmthe estimates were not influenced by the presence of inhibitors of proteolytic enzymes. Supernatants, obtained after keeping the lymphocytes in culture for 16 h, were analyzed by the same methods. Again, matching amounts of a1 and L-chain were found in supernatants from nonfractionated as well as from B cell-depleted populations. The results obtained indicate that T cells do not ...
Davies P, Katzman R, Terry RD. Reduced somatostatin-like immunoreactivity in cerebral cortex from cases of Alzheimer disease and Alzheimer senile dementa ...
Introduction: Chromogranin A (CgA) is not very accurate for the diagnosis of gastric carcinoid type1 (GC1). Clinical interpretation of CgA results may be affected by the heterogeneity between CgA assays. The commercial CgA assay, DAKO (DAKO, Denmark A/S, Glostrup, Denmark) is an ELISA which recognizes a 23 kD C terminal fragment of CgA; the Imperial Supra-regional Assay Service radioimmunoassay (SAS Hammersmith Hospital, Imperial College, London) is a competitive radioimmunoassay raised against the whole pancreastatin molecule ...
Uotila, M; Engvall, E; and Ruoslahti, E, Monoclonal antibodies in radioimmunoassay of alphafetoprotein. Abstr. (1980). Subject Strain Bibliography 1980. 2317 ...
Reuter, A M ; Ketelslegers, Jean-Marie ; Hendrick, Jean-Pierre ; Franchimont, P.. Radioimmunoassay of protein hormones: principles and methodology.. In: Hormone research, Vol. 9, no. 6, p. 404-21 (1978 ...
In 21 normal fasting subjects basal serum-pancreatic-polypeptide (H.P.P.), as measured by a specific radioimmunoassay, was 43 +/- 4 pmol/l (mean +/- S.E.M). This basal concentration was of the same magnitude as that of other gastrointestinal peptide hormones. In normal subjects the H.P.P. response t …
[113 Pages Report] Radioimmunoassay Market report categories the Global market by Application (Research, Clinical Diagnostics), Type (Reagents & Kits, Analyzers), End User (Hospital, Pharmaceutical Industry, Academics, Clinical Diagnostic Labs) & Geography
VWR Tube Racks for Radioimmunoassay Tubes, Polypropylene - Model 60985-250 - Case of 6 : Designed for easy pipetting and storage, these racks provide
The U937 cell line has been shown to generate tumour fibrosis factor α (TNF-α) in response to soluble stimuli such as PMA and LPS, but only after treatment with GM-CSF. We report here the generation of TNF-α from U937 cells following phagocytosis of opsonised zymozan particles without the need for pre-treatment with GM CSF. The release of TNF-α from U937 cells was demonstrated by a specific radioimmunoassay, L929 cell killing and neutrophil priming. The biological activities in the cell supernatant were inhibited by TNF-α affarserum. Phagocytosis was required for TNF-α production. Non-opsonised zymozan or latex particles which were not phagocytosed or pretreatment with cytochalasin B, which inhibited phagocytosis of opsonised zymozan particles, all failed to trigger TNF-α production. Phagocytosis failed to trigger detectable IL-1 generation, and production of IL-6 was insufficient to produce biological effects on neutrophils. The U937 supernatant thus provides a source of human TNF-α ...
Research Report on Europe Radioimmunoassay Market Report 2017. The Report includes market price, demand, trends, size, Share, Growth, Forecast, Analysis & Overview.
Middle East and Africa Radioimmunoassay Market is poised to reach USD 38.84 million by 2021 - 142-page report by Market Data Forecast
Endoscopy Diagnostic Products and Services: Developed a patented technology for a dual IR/visible endoscope and borescope compatible with several off the shelf IR cameras.
Previously, heterozygous INS gene mutations had been detected in adult patients with so-called familial hyperinsulinemia or hyperproinsulinemia who presented with variable phenotypes (mild diabetes or even hypoglycemia) and high serum levels of radioimmunoassayable insulin or proinsulin-like material. More recently, INS mutations have been found to be associated with neonatal- and infancy-onset diabetes (1-3). We demonstrated that mutant insulins with proteotoxic effect cannot be secreted when expressed in HEK 293 cell line (1), and it is likely that SB8 and SSignal23 are also retained in the endoplasmic reticulum. Nevertheless, the patient bearing the mutation in the signal peptide shows a milder clinical course, and we cannot exclude that SSignal23 preproinsulin may be partially processed and secreted. Present knowledge indicates that insulin mutations with a proteotoxic effect cause apoptosis of the pancreatic β-cell (1), a process that in most patients takes several months after birth (1-3) ...
[Testing Process] This kit employs Double Antibody Sandwich Technique. The principle of Double Antibody Sandwich is based on characteristics of the tested antigen with more than two valances which can identify coated antibody and detection antibody...
Invitrogen Anti-ApoA1 Monoclonal (6001), Catalog # MIA1401. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP), ELISA (ELISA) and Radioimmune assays (RIA) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 1 mg purified antibody (5 mg/mL).
Definition : Radioimmunoassay analyzers that require the human manipulative effort (e.g., manual insertion of reagents and samples) in all steps of each test, including manual pipetting, control of incubation, separation, and counting. These tests are usually performed using manufacturers kits, which include the unlabeled and radioactively labeled ligands, the binder, and the calibration standards. Most current manual radioimmunoassay analyzers include computerized data-processing (data reduction) devices.. Entry Terms : Radioimmunoassay Analyzers, Manual. UMDC code : 16865 ...
Company profile & key executives for Caribbean Dental Products Corp (9969644Z:-) including description, corporate address, management team and contact info.
This protocol can be used to measure the concentration of steroid hormones in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 4-5 days to complete, is described below. Prepared January 1998 by Joe Casto, modified December 2005 by Jodie Jawor and May 2010 by Danielle Whittaker ...
INTRODUÇÃO: A mortalidade das vítimas de queimaduras tem diminuído nos últimos anos e a lesão inalatória tornou-se a principal causa de morte. As consequências da inalação por fumaça resultam de uma resposta inflamatória que envolve vários mediadores. A proteína 1 relacionada a uteroglobulina (UGRP1) é uma secretoglobulina antinflamatória específica do pulmão que pode desempenhar um papel na inflamação pulmonar. OBJETIVO: Investigar se a expressão precoce da UGRP1 na inalação de fumaça está relacionada com a severidade da injúria inalatória. MÉTODOS: Nós estudamos 16 pacientes críticos vítimas de queimaduras. Todos os pacientes foram mecanicamente ventilados por Síndrome da Angústia Respiratória do Adulto (SARA) secundária a injúria por inalação de fumaça. Uma amostra de sangue foi coletada entre a admissão a UTI até 24h após a injúria inalatória. O grau da injúria por inalação foi avaliado em todos os pacientes. RESULTADOS: A média de idade dos ...
Principal Investigator:HASEGAWA Yoshihisa, Project Period (FY):1989 - 1991, Research Category:Grant-in-Aid for Developmental Scientific Research., Research Field:畜産学(含草地学)
Колхицин Bremner W. J., Paulsen С. A. Colchicine and testicular function in man. - New Engl. J. Med., 1976, 294, 1384.Ertel M. H., Miller J. C, Akgun S., Wallace S. L. Radioimmunoassay for colchicine in plasma and urine. - Science, 1976, 193, 233.Goldfinger S. E. Colchicine for familial Meditarranean ...
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Baseline Sit N Reach Trunk Flexibility Box - Deluxe - Get the lowest price on Baseline Sit N Reach Trunk Flexibility Box - Deluxe, online at AllegroMedical.com.
Near-complete recovery of the radioactive dose was obtained in this study (95.8% of dose). Methods were developed to maximize extraction efficiency before quantitative metabolite profiling. In seven patients, a mean total of 92.2% of dose was quantitatively profiled. This included a loss during extraction of 11.1% of dose, primarily from feces. Our experience during the development of these extraction methods indicates that this loss was nonspecific, and, in our opinion, does not represent an unextracted metabolite.. Other investigators have attempted to quantify CPT-11 and metabolites in urine, bile, and feces with nonradiometric methods (Lokiec et al., 1995; Sparreboom et al., 1998). These studies generally obtained low total recoveries of drug-related materials. During this study, we discovered that there was a significant risk of precipitation of drug-related material in feces, bile, and urine during freezing. This made necessary the appropriate dilution of the specimen to assure dissolution ...
ऐसा नहीं है कि केवल हमारी अस्थायी रबर लुब्रिकंट की श्रृंखला ही हमारी अलग पहचान बनाती है - बल्कि हमारी सेवा भी हमें अलग पहचान दिलाती है। IPC में हम हमारे ग्राहकों को एक परिवार की तरह महसूस कराने के लिए प्रयासरत रहते हैं और इसका अर्थ है हम ऐसा रिश्ता बनाते हैं जिसमें आप आश्वस्त हो यह सुनिश्चित होता है। इसलिए, हम अपने सभी ग्राहकों को निम्न लाभ प्रस्तुत करते हैं:. मुफ़्त नमूने. खरीदने से पहले ...
화학 제조업체로서 당사는 환경과 고객에 대한 책임을 매우 진지하게 받아들입니다.. 환경적 입장에서 당사의 수성 윤활제와 세척제는 기존에 사용되던 부식물, 인산염, 용제, 석유 증류액 및 기타 유해한 화학물질을 대체할 수 있습니다. 당사는 뉴저지 벌링턴 공장에서 이들 제품을 개발, 생산합니다. IPC는 ISO 9001 인증을 받았으며 무방류 정책을 고수합니다. 또한 당사는 제품 및 시설의 품질과 안전성을 지속적으로 개선하기 위해 환경 및 규제 동향과 모범 사례를 자세히 숙지하는 데 전념합니다.. 이와 같은 지속적인 개선 약속은 당사 고객의 요구에까지 확장됩니다. 당사는 사내 연구소를 활용해 연구, 개발 및 시험을 계속 수행하면서 모든 제품을 고객의 의견과 변화하는 산업 표준에 기반하여 개발, 혁신합니다. 당사는 고객과의 관계를 소중히 여기며 ...
(A) Determination of hormones By radioimmunoassay (RIA) can determine steroid hormone, but also determination of pituitary hormones. Specificity of immune response strong isotope sensitivity, accuracy is high, it has been widely used. Infer
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Growth Regulated Oncogene Gamma (GROg) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species ...
Buy IA2 recombinant protein (MBS8506800) product datasheet at MyBioSource, Recombinant Proteins. Application: ELISA (EIA), CLIA, Radioimmunoassay (RIA), immunohistochemistry (IHC), Western Blot (WB)
The licensing agreement marks the first time that a company other than Exiqon will use the locked nucleic acid technology to develop diagnostic products.
Determinations were via radioimmunoassay. Levels of sex hormones and SHBG during pregnancy in women. The dashed vertical lines ... Determinations were via radioimmunoassay. Levels of progesterone and estrogen rise continually throughout pregnancy, ...
182-. ISBN 978-0-323-16027-8. Bernard Jaffe (2 December 2012). Methods of Hormone Radioimmunoassay. Elsevier Science. pp. 196 ... Bernard Jaffe (2 December 2012). Methods of Hormone Radioimmunoassay. Elsevier Science. pp. 196-. ISBN 978-0-323-16126-8. " ...
Purification, characterization, and radioimmunoassay". J. Biol. Chem. 261 (34): 16264-9. PMID 2877986. Biology portal v t e. ...
Yalow RS, Glick SM, Roth J, Berson SA (November 1964). "Radioimmunoassay of human plasma ACTH". The Journal of Clinical ...
It used radioimmunoassay for the determinations, with no mention of chromatographic separation. Estradiol levels following an ... Assays were performed using radioimmunoassay. Source was Wiemeyer et al. (1986). Estradiol and prolactin levels after the most ... Assays were performed using radioimmunoassay. Source was Recio et al. (1986). Estradiol levels after a single intramuscular ... Assays were performed using radioimmunoassay. Source was Garza-Flores et al. (1989). Simplified curves of estradiol levels ...
One of Buster's earliest research studies focused on steroid hormone radioimmunoassay (RIA). He reported an RIA for the ... Buster JE, Abraham GE: Radioimmunoassay of plasma dehydroepiandrosterone sulfate. Analyt Lett 5:543:551, 1972. Buster JE, ... "Comparison Between Four Radioimmunoassays for Plasma Estriol". Analytical Letters. 7 (2): 119-123. doi:10.1080/ ... "Radioimmunoassay of Plasma Dehydroepiandrosterone Sulfate". Analytical Letters. 5 (8): 543-551. doi:10.1080/00032717208062119. ...
Assays were performed using radioimmunoassay. Source was Shaw et al. (1975). Estradiol levels after single intramuscular ... Assays were performed using radioimmunoassay with chromatographic separation. Source was Oriowo et al. (1980). Estradiol levels ...
125I is frequently used in radioimmunoassays because of its relatively long half-life (59 days) and ability to be detected with ... Gilby ED, Jeffcoate SL, Edwards R (July 1973). "125-Iodine tracers for steroid radioimmunoassay". The Journal of Endocrinology ...
E1S using radioimmunoassay (RIA) have been reported to be 0.96 ± 0.11 ng/mL in men, 0.96 ± 0.17 ng/mL during the follicular ... convenient radioimmunoassay of estrone sulfate". Clin. Chem. 44 (2): 244-9. doi:10.1093/clinchem/44.2.244. PMID 9474019. Cowie ... measured by a direct radioimmunoassay, and fate of exogenously injected estrone sulfate". Horm Res. 27 (2): 61-8. doi:10.1159/ ...
Fujii Y, Teranishi M, Nakada K, Yamazaki M, Kishida S, Miyabo S (September 1988). "Radioimmunoassay of 2-methoxyestriol in ...
"Determination of tiamenidine in biological specimens by radioimmunoassay". Arzneimittel-Forschung. 31 (3): 419-24. PMID 7194666 ...
Sensitive radioimmunoassay techniques were developed for diagnostic and medical use. Natural radioisotopes were used as tracers ...
... and vaccinia antisera by radioimmunoassay" (PDF). Bulletin of the World Health Organization. 5 (5): 613-623. PMC 2366687. PMID ...
The measurement of AGA is done with ELISA or radioimmunoassay. Such tests measure the level of AGA relative to a standard, such ...
John, H; Cammann, K; Schlegel, W (1998). "Development and review of radioimmunoassay of 12-S-hydroxyheptadecatrienoic acid". ...
Radioimmunoassay was first described in a scientific paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960. As ... In radioimmunoassay, the radioactivity provides the signal, which indicates whether a specific antigen or antibody is present ... Before the development of the ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using ... A suitable alternative to radioimmunoassay would substitute a nonradioactive signal in place of the radioactive signal. When ...
Matsukura, A.; Nakazawa, Y.; Hama, S. (November 1989). "[A shortened method for the assay with Centocor radioimmunoassay kit ...
Negoro N, Kanayama Y, Takeda T, Koda S, Inoue T (July 1986). "A solid-phase radioimmunoassay for the detection of nRNP immune ...
In the 1950s radioimmunoassay was developed by Solomon Berson and Rosalyn Yalow. Dr. Yalow was co-winner of the 1977 Nobel ... Berson had already died so was not eligible). Radioimmunoassay was used extensively in clinical medicine but more recently has ... At that time, the specialty include all of the uses of radioisotopes in medicine - radioimmunoassay, diagnostic imaging, and ... As use of and experience with radioisotopes became more widespread in medicine, radioimmunoassay generally transferred from ...
17beta-diol in human plasma by radioimmunoassay". Acta Endocrinologica. 88 (4): 778-86. doi:10.1530/acta.0.0880778. PMID 581118 ...
Radioactive isotopes can be incorporated into immunoassay reagents to produce a radioimmunoassay (RIA). Radioactivity emitted ... ELISA MELISA CEDIA Immunoscreening Lateral flow test Magnetic immunoassay Radioimmunoassay Surround Optical Fiber Immunoassay ( ...
Hiroi, M.; Stanczyk, F.Z.; Goebelsmann, U.; Brenner, P.F.; Lumkin, M.E.; Mishell, D.R. (1975). "Radioimmunoassay of serum ... both using radioimmunoassay as well. Treatment of postmenopausal women with 2.5 or 5 mg/day MPA in combination with estradiol ... peak levels of MPA were 3.4 to 4.4 ng/mL within 1 to 4 hours of administration of 10 mg oral MPA using radioimmunoassay. ... mg MPA has been found to result in peak MPA levels of 1.2 to 5.2 ng/mL within 2 hours of administration using radioimmunoassay ...
Fujii Y, Teranishi M, Nakada K, Yamazaki M, Kishida S, Miyabo S (February 1992). "Radioimmunoassay for the determination of 2- ...
Blood was taken and analyzed after death by radioimmunoassay and liquid chromatography/mass spectrometry. This study showed the ...
Kumar N, Didolkar AK, Ladd A, Thau R, Monder C, Bardin CW, Sundaram K (November 1990). "Radioimmunoassay of 7 alpha-methyl-19- ...
1983). "Anticardiolipin antibodies: detection by radioimmunoassay and association with thrombosis in systemic lupus ...
Radioimmunoassays require sophisticated apparatus and special radiation precautions and are expensive. Organon International ... Direct measurement of antigens, such as hCG, was made possible after the invention of the radioimmunoassay in 1959. ...
They developed the radioimmunoassay, which gave very good results, and published their findings in 1960. They were able to ... for their joint work on the radioimmunoassay. Born in New York City, Berson was a keen musician and chess player. He graduated ... for their joint work on the radioimmunoassay. Rall JE. Solomon A. Berson. In "Biographical Memoirs". National Academy of ...
Protein radioimmunoassay is a biomolecular method that identifies human bones and eliminates any nonhuman bones. Once the ...
The invention of radioimmunoassay techniques revolutionized behavioral endocrinology, allowing scientists to see and quantify ...
steps in radioimmunoassay technique Radioimmunoassay at the US National Library of Medicine Medical Subject Headings (MeSH) ... A radioimmunoassay (RIA) is an immunoassay that uses radiolabeled molecules in a stepwise formation of immune complexes. A RIA ... A radioallergosorbent test (RAST) is an example of radioimmunoassay. It is used to detect the causative allergen for an allergy ... Classically, to perform a radioimmunoassay, a known quantity of an antigen is made radioactive, frequently by labeling it with ...
radioimmunoassay (third-person singular simple present radioimmunoassays, present participle radioimmunoassaying, simple past ... radioimmunoassay (plural radioimmunoassays) (abbreviated as RIA). *A procedure that uses the binding of a radioactively labeled ... Retrieved from "https://en.wiktionary.org/w/index.php?title=radioimmunoassay&oldid=45403241" ...
... awarded for her development of radioimmunoassay (RIA), an extremely sensitive technique for measuring minute quantities of ... Other articles where Radioimmunoassay is discussed: Rosalyn S. Yalow: … ... been significantly increased by a radioimmunoassay (RIA) procedure. In this procedure a "known" antigen is overlayed on a ... awarded for her development of radioimmunoassay (RIA), an extremely sensitive technique for measuring minute quantities of ...
... there were already many examples of how radioimmunoassays improved diagnostic laboratory methods. Although mildly disadvantaged ... By the 1970s, there were already many examples of how radioimmunoassays improved diagnostic laboratory methods. Although mildly ... radioimmunoassays offered a technology which had the potential to enhance the sensitivity of antigen or antibody detection. ... Cimolai N. (1999) Radioimmunoassay. In: Serodiagnosis of the Infectious Diseases. Springer, Boston, MA. * DOI https://doi.org/ ...
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title = {Radioimmunoassay of synthetic steroids}. author = {Raynaud, J -P, Bucourt, R, and Salmon, J}. abstractNote = {The ... Raynaud, J -P, Bucourt, R, and Salmon, J. Radioimmunoassay of synthetic steroids. France: N. p., 1975. Web. ... The sensitivity of a radioimmunoassay depends on the intrinsic association constant of the interaction between ligand and ... 62 RADIOLOGY AND NUCLEAR MEDICINE; STEROIDS; RADIOIMMUNOASSAY; ANTIGEN-ANTIBODY REACTIONS; IMMUNE SERUMS; IODINE 125; BETA ...
New techniques in tumor localization and radioimmunoassay.. [Millard N Croll; Hahnemann Medical College and Hospital of ... New techniques in tumor localization and radioimmunoassay.. Author:. Millard N Croll; Hahnemann Medical College and Hospital of ... schema:name "New techniques in tumor localization and radioimmunoassay."@en ;. schema:productID "934617" ;. schema:publication ... Add tags for "New techniques in tumor localization and radioimmunoassay.". Be the first. ...
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A radioimmunoassay made to detect alpha-fetoprotein, a tumor marker often found in higher concentrations in those with liver ... A radioimmunoassay made to detect alpha-fetoprotein, a tumor marker often found in higher concentrations in those with liver ... Development of a Radioimmunoassay for ±-Fetoprotein. Demonstration of ±-Fetoprotein in Serum of Healthy Human Adults." ...
Use of a radioimmunoassay to quantify thrombospondin Message Subject (Your Name) has forwarded a page to you from Blood Journal ... Use of a radioimmunoassay to quantify thrombospondin. SD Saglio and HS Slayter ... Results of radioimmunoassay procedures applied to samples containing thrombospondin indicated that reliable values are obtained ... Use of a radioimmunoassay to quantify thrombospondin. Blood, 59(1), 162-166. Accessed July 15, 2019. Retrieved from http://www. ...
113 Pages Report] Radioimmunoassay Market report categories the Global market by Application (Research, Clinical Diagnostics), ... Table 3 Radioimmunoassay (RIA) Market, By Type, 2013-2020 (USD Million). Table 4 Radioimmunoassay (RIA) Market, By End-User , ... Table 54 Asia: Radioimmunoassay Market, By End User, 2013-2020 (USD Million). Table 55 China: Radioimmunoassay (RIA) Market, By ... Table 8 Radioimmunoassay Market, By Type, 2013-2020 (USD Million). Table 9 Radioimmunoassay (RIA) Reagents & Kits Market, By ...
Erythropoietin in renal transplant recipients: studies based on recombinant human erythropoietin radioimmunoassay.. Ward HJ1, ...
... in the brain of several species has been investigated by SDS-gel electrophoresis and by radioimmunoassay. This assay had a ... As determined with this radioimmunoassay, MAP2 accounted for about 10% of the porcine brain microtubule protein and 1% of the ... Comparative measurement by radioimmunoassay of the brain microtubule-associated protein MAP2. ... in the brain of several species has been investigated by SDS-gel electrophoresis and by radioimmunoassay. This assay had a ...
Radioimmunoassay and Related Techniques to Improve Artificial Insemination Programmes for Cattle Reared under Tropical a .... ... Application of Radioimmunoassay in Improving the Reproductive Management of Smallholder Dairy Cattle .... IAEA TECDOC - 1571 ... Studies on the Reproductive Efficiency of Cattle Using Radioimmunoassay Techniques Proceedings of the Final Research ... INTERNATIONAL ATOMIC ENERGY AGENCY, Studies on the Reproductive Efficiency of Cattle Using Radioimmunoassay Techniques, IAEA, ...
A solid phase radioimmunoassay for adrenal antibodies is described. In the assay plastic tubes coated with adrenal microsomes ( ... A solid phase radioimmunoassay for adrenal antibodies is described. In the assay plastic tubes coated with adrenal microsomes ( ... In the group of 30 patients with Addisons disease 19 had positive results in adrenal antibody radioimmunoassay (RIA). ...
Zirker, D. K., Krueger, G. G., Meikle, A. W.: Percutaneous absorption of dexamethasone estimated by a plasma radioimmunoassay. ... Frölich, M., Thermoshuizen, W., Kenter, E., Moolenaar, A. J.: Steroid radioimmunoassay: contribution of standards to blank ... Plasma levels of triamcinolone acetonide as determined by radioimmunoassay after topical application. ... Percutaneous absorption of bethamethasone-17-benzoate measured by radioimmunoassay. J. invest. Derm.67, 279-282 (1976)Google ...
Type: Radioimmunoassay Protocol Abstract:. This protocol can be used to measure the concentration of steroid hormones in small ... Protocol for steroid radioimmunoassay of plasma. Joe Casto, Jodie Jawor, Danielle Whittaker ...
Parathyroid Hormone Radioimmunoassay JOEL F. HABENER, M.D.; GINO V. SEGRE, M.D. ... Parathyroid Hormone Radioimmunoassays in the Differential Diagnosis of Hypercalcemia Due to Primary Hyperparathyroidism or ... Parathyroid Hormone Radioimmunoassay. Ann Intern Med. 1979;91:782-785. doi: 10.7326/0003-4819-91-5-782 ... illustrates both the assets and limitations of commercially available radioimmunoassays for immunoreactive parathyroid hormone ...
We have developed a rapid and sensitive micro-radioimmunoassay for the detection of cellular cAMP. The assay employs a simple ... We have developed a rapid and sensitive micro-radioimmunoassay for the detection of cellular cAMP. The assay employs a simple ...
A radioimmunoassay has been developed for the measurement of serum myoglobin in order to evaluate the time-course and frequency ... Radioimmunoassay of serum myoglobin appears to be useful and sensitive test for the early detection of myocardial infarction. ... Radioimmunoassay of myoglobin in human serum. Results in patients with acute myocardial infarction.. ... Radioimmunoassay of myoglobin in human serum. Results in patients with acute myocardial infarction.. ...
Geographically, the Asian Radioimmunoassay market is expected to grow at a CAGR of 4.5% from 2015 to 2020. In terms of high ... rise in outsourcing of Radioimmunoassay and increase in Research of Radioimmunoassay in Oncology. ... The demand for Radioimmunoassay products has continued to remain as growing across all geographies. This can be attributed to ... The Asian Radioimmunoassay Market report defines and segments the concerned market with analysis and forecast of revenue. This ...
The global radioimmunoassay market is estimated to surpass $520.6 million by 2027, exhibiting a CAGR of 3.5% from 2020 to 2027. ... The report aims to offer a clear picture of the current scenario and future growth of the global Radioimmunoassay Market... ... The global Radioimmunoassay Market market is segmented on the basis of the following:. Global Radioimmunoassay Market Market By ... Global Radioimmunoassay Market Market By Regions:. North America (U.S, Canada, and Mexico.)Europe (Germany, UK, France, Spain, ...
A Rapid Solid Phase Radioimmunoassay for Staphylococcal B Enterotoxin. William S. Collins II, Joseph F. Metzger and Anna D. ... A Rapid Solid Phase Radioimmunoassay for Staphylococcal B Enterotoxin Message Subject (Your Name) has forwarded a page to you ... This paper describes a rapid sensitive solid phase radioimmunoassay procedure for staphylococcal enterotoxin B, which can be ...
Automated Radioimmunoassay of Plasma Oestriol: Unexpected Problems M. G. R. Hull M. G. R. Hull ... The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens Biochem J (November, 1975) ... The direct radioimmunoassay of oestrogen glucuronides in human female urine Biochem J (February, 1979) ... M. G. R. Hull, P. P. Monro; Automated Radioimmunoassay of Plasma Oestriol: Unexpected Problems. Clin Sci Mol Med 1 January 1975 ...
Radioimmunoassay: a probe for the fine structure of biologic systems Message Subject. (Your Name) has forwarded a page to you ...
A Radioimmunoassay of Bovine Factor X ARTHUR D. DAVIS; ARTHUR D. DAVIS ... A radioimmunoassay for chicken avidin. Comparison with a [ 14 C]biotin-binding method Biochem J (November,1978) ... ARTHUR D. DAVIS, RICHARD F. MURPHY, DONALD T. ELMORE; A Radioimmunoassay of Bovine Factor X. Biochem Soc Trans 1 August 1974; 2 ... Using Radioimmunoassays for Pro-Atrial Natriuretic Peptide (31-67), Pro-Atrial Natriuretic Peptide (1-30) and Atrial ...
Two solid-phase radioimmunoassays have been developed for the detection of myoglobin in serum and urine. The sensitivity of the ... Radioimmunoassays of human myoglobin in serum and urine Scand J Clin Lab Invest. 1979 Feb;39(1):37-46. doi: 10.3109/ ... Two solid-phase radioimmunoassays have been developed for the detection of myoglobin in serum and urine. The sensitivity of the ...
Radioimmunoassay of Prostaglandin A. Intrarenal PGA2 as a Factor Mediating Saline-Induced Natriuresis. AHMAD A. ATTALLAH, JAMES ... A highly specific, sensitive (25 pg) radioimmunoassay for prostaglandin A is described; the assay is characterized by high ...
  • The global radioimmunoassay market was valued at $345.2 Million in 2014 and is expected to reach $428.4 Million in 2020 at a CAGR of 3.5% from 2015-2020. (marketsandmarkets.com)
  • In 2014, the reagents & kits segment contributed the largest share of 82.9% to the global radioimmunoassay market. (marketsandmarkets.com)
  • The global radioimmunoassay market has been categorized into major geographical regions, including Europe, North America, Asia, and the Rest of the world. (marketsandmarkets.com)
  • Pune, New York, USA, November 25 2020 (Wiredrelease) Research Dive -:The global radioimmunoassay market is estimated to surpass $520.6 million by 2027, exhibiting a CAGR of 3.5% from 2020 to 2027. (pharmiweb.com)
  • The report aims to offer a clear picture of the current scenario and future growth of the global Radioimmunoassay Market market. (pharmiweb.com)
  • The report articulates the key opportunities and factors propelling the global Radioimmunoassay Market market growth. (pharmiweb.com)
  • The report reveals various statistics such as predicted market size and forecast by analyzing the major factors and by assessing each segment of the global Radioimmunoassay Market market. (pharmiweb.com)
  • Furthermore, the report profiles top players active in the global Radioimmunoassay Market market. (pharmiweb.com)
  • The global radioimmunoassay market is expected to reach $428.4 million by 2020, at a CAGR of 3.5% during the forecast period from 2015 to 2020. (pitchengine.com)
  • The rise in number of blood donations in blood banks leads to the tracking & screening of blood for infections or diseases such as hepatitis and leukemia using radioimmunoassay, which in turn is likely to augment the global radioimmunoassay market during the forecast period. (transparencymarketresearch.com)
  • North America accounted for the largest share of the global radioimmunoassay market in 2019, as increasing number of cancer cases in the U.S. and other infectious disease such as COVID-19 and influenza have increased the need for radioimmunoassay analyzers, kits, and reagents for the early diagnosis of disease. (transparencymarketresearch.com)
  • However, high cost of equipment required for procedures and hazardous radioactive material in the assay are factors that are expected to hamper growth of the global radioimmunoassay market. (coherentmarketinsights.com)
  • Radioimmunoassay of myoglobin in human serum. (jci.org)
  • A radioimmunoassay has been developed for the measurement of serum myoglobin in order to evaluate the time-course and frequency of myoglobinemia in patients with acute myocardial infarction. (jci.org)
  • Radioimmunoassay of serum myoglobin appears to be useful and sensitive test for the early detection of myocardial infarction. (jci.org)
  • Two solid-phase radioimmunoassays have been developed for the detection of myoglobin in serum and urine. (nih.gov)
  • Utility of the Radioimmunoassay of Serum Thyrotropin. (annals.org)
  • A highly specific and sensitive radioimmunoassay (RIA) for the measurement of plasma and pituitary growth hormone (GH) levels in salmonid fishes was developed using an anti-serum raised in rabbit against chum salmon (Oncorhynchus keta) GH (sGH). (semanticscholar.org)
  • The procedure involves rapid separation of 3TC-TP by using Sep-Pak cartridges, dephosphorylation to 3TC by using acid phosphatase, and measurement by radioimmunoassay using a newly developed anti-3TC serum. (asm.org)
  • Polyspecific antiserum against total rat serum proteins was used to develop specific and sensitive radioimmunoassays for fetuin and bikunin, two minor protein components of rat plasma. (uni-muenchen.de)
  • The monoclonal antibody systems were also compared with the homologous enterotoxin-specific polyclonal serum for the detection of enterotoxin by the competitive radioimmunoassay (RIA). (asm.org)
  • Serum human leptin concentrations can be accurately and precisely measured by this new radioimmunoassay. (viamedica.pl)
  • A solid phase radioimmunoassay for adrenal antibodies is described. (biomedsearch.com)
  • Radioimmunoassay technique is basically used for measuring the concentration of specific antigens by using specific antibodies. (pitchengine.com)
  • Radioimmunoassay technique is basically used to measure the concentration of specific antigens by using specific antibodies. (pitchengine.com)
  • A solid-phase radioimmunoassay (RIA) was used for the detection and quantitation of immunoglobulin class-specific antibodies to Mycoplasma pulmonis in mouse sera. (asm.org)
  • Radioimmunoassays specific for fructose-1, 6-diphosphate aldolase isozymes were developed for the quantification of human aldolase A, B and C. The method is a double-antibody radioimmunoassay using radioiodinated purified aldolase A, B and C as ligand, chicken antibodies to aldolase A, B and C, and rabbit antibodies to chicken IgG. (gallusimmunotech.com)
  • Radioimmunoassay can be used to measure or determine very small quantity (e.g. nanogram, picogram) of substances such as antigen, antibodies, hormones, and drugs. (transparencymarketresearch.com)
  • Radioimmunoassays are based on high sensitivity in vitro assay techniques to measure concentrations of antigens by the use of antibodies or alternatively to detect antibodies for a specific antigen. (svarlifescience.com)
  • Radioimmunoassay (RIA) is a very sensitive in vitro assay technique used to measure concentrations of antigens (for example, hormone levels in blood) by use of antibodies. (abbexa.com)
  • We present a double-antibody radioimmunoassay for determining human interleukin-6 (IL-6) in biological fluids. (aaccjnls.org)
  • A double-antibody radioimmunoassay method for prostate-specific acid phosphatase (PAP) is presented. (elsevier.com)
  • Although mildly disadvantaged because of the need for special conditions related to the handling and measurement of radioactive isotopes, radioimmunoassays offered a technology which had the potential to enhance the sensitivity of antigen or antibody detection. (springer.com)
  • A micro-radioimmunoassay for the measurement of intracellular cAMP. (biomedsearch.com)
  • Insulin autoantibodies measured by radioimmunoassay methodology are more related to insulin-dependent diabetes mellitus than those measured by enzyme-linked immunosorbent assay: results of the Fourth International Workshop on the Standardization of Insulin Autoantibody Measurement. (nih.gov)
  • In 1959 Yalow and Berson perfected their measurement technique and named it radioimmunoassay (RIA). (discoveriesinmedicine.com)
  • Measurement of medroxyprogesterone acetate (Provera) by radioimmunoassay. (springer.com)
  • Increasing significance of research and development in medical science leads to usage of radioimmunoassay in pharmaceutical & biotechnological industries, medical research labs, and contract research organizations (CROs), which is anticipated to propel the demand for analyzers, kits, and reagents. (transparencymarketresearch.com)
  • Radioimmunoassay analyzers that require the human manipulative effort (e.g., manual insertion of reagents and samples) in all steps of each test, including manual pipetting, control of incubation, separation, and counting. (optometricmanagement.com)
  • Reagents consisting of substances of known concentrations intended for quality control in a diverse range of sensitive and specific radioimmunoassay tests. (optometricmanagement.com)
  • Reagents used for radioimmunoassay quality control may be specific for a substance or, more frequently, appropriate for quality control for a whole set of similar substances (e.g., proteins, lipids) that are found in human specimens. (optometricmanagement.com)
  • The Middle East and Africa Radioimmunoassay Market is broadly classified into Reagents and Kits Market and Analyzers Market based on type, based on End User into Hospital, Pharmaceutical Industry and CRO, Academics and Clinical Diagnostic Laboratories, on the basis of Application into Research and Clinical Diagnostics. (marketdataforecast.com)
  • Classically, to perform a radioimmunoassay, a known quantity of an antigen is made radioactive, frequently by labeling it with gamma-radioactive isotopes of iodine, such as 125-I, attached to tyrosine. (wikipedia.org)
  • Comparison of radioimmunoassay and enzyme immunoassay kits for detection of Legionella pneumophila serogroup 1 antigen in both concentrated and nonconcentrated urine samples. (asm.org)
  • We evaluated a commercial enzyme immunoassay (EIA) kit for detection of Legionella pneumophila serogroup 1 soluble antigen by comparing it to radioimmunoassay (RIA), using both concentrated and nonconcentrated urine samples. (asm.org)
  • Radioimmunoassay for Gram-negative bacterial lipopolysaccharide O antigens: influence of antigen solubility. (asm.org)
  • The two examples demonstrate that, in general, it will be possible to develop a specific and sensitive radioimmunoassay with antiserum raised against a crude antigen preparation, such as a body fluid or a tissue extract, provided that a minute amount of pure antigen is available for preparing the radioiodinated antigen. (uni-muenchen.de)
  • Radioimmunoassay (RIA) is an in vitro assay for measuring antibody levels by introducing a radiolabeled antigen and determining the subsequent radioactivity of the antibody component. (transparencymarketresearch.com)
  • In this technique, antibody and antigen bind to one another and the amount of bound antigen is measured using radioimmunoassay. (coherentmarketinsights.com)
  • The three main factors involved in radioimmunoassay include bound antigen, a radio labelled antigen, and a pure antigen, along with a separation unit to estimate the delivery of radioactivity in free and bound antigens. (coherentmarketinsights.com)
  • Radioimmunoassay (RIA) is a method employing radioactive isotopes to label either the antigen or antibody. (encyclopedia.com)
  • Radioimmunoassay (RIA) is an in-vitro assay technique that is used to measure the concentration of antigens in blood. (marketsandmarkets.com)
  • Radioimmunoassay (RIAs) is a very sensitive medical technique used to measure concentrations of antigens such as quantitating the binding, or the inhibition of binding, of a radio-labeled substance to an antibody which help your doctor to determine that the infertility is not or caused by hormone imbalance during menstrual cycle. (worldwidehealth.com)
  • Radioimmunoassay (RIA) is one of the most frequently used in-vitro assay techniques to measure the concentration of antigens in blood. (coherentmarketinsights.com)
  • A radioimmunoassay for the detection of adrenal autoantibodies. (biomedsearch.com)
  • Insulin autoantibodies measured by radioimmunoassay methodology are more related to insulin-dependent diabetes mellitus than those measured by enzy. (nih.gov)
  • The sensitivity of a radioimmunoassay depends on the intrinsic association constant of the interaction between ligand and antibody. (osti.gov)
  • misc{etde_7139654, title = {Radioimmunoassay of synthetic steroids} author = {Raynaud, J -P, Bucourt, R, and Salmon, J} abstractNote = {The sensitivity of a radioimmunoassay depends on the intrinsic association constant of the interaction between ligand and antibody. (osti.gov)
  • Radioimmunoassay is used owing to its high sensitivity, which can analyze nanomolar and picomolar concentrations of hormones in biological fluids. (marketsandmarkets.com)
  • Sensitivity and specificity of passive hemagglutination (RCA), solid phase radioimmunoassay (RIA), and immunoelectroosmophoresis (IEOP) were compared under experimental and clinical conditions. (asm.org)
  • A radioimmunoassay (RIA) for dUTP, with a sensitivity of 3.78 fmol, has been developed. (ox.ac.uk)
  • Over the years, the importance of radioimmunoassay in the field of medical diagnostics has increased due to various factors such as specificity of results, accuracy, sensitivity, and precision. (coherentmarketinsights.com)
  • The radioimmunoassay format is inherently associated with a high analytical sensitivity and in combination with a specific detection antiserum, result in a high performing assay. (svarlifescience.com)
  • Radioimmunoassay is an old assay technique, but it is still a widely used assay and continues to offer distinct advantages in terms of simplicity and sensitivity. (svarlifescience.com)
  • Most current manual radioimmunoassay analyzers include computerized data-processing (data reduction) devices. (optometricmanagement.com)
  • The growing aging population and rise in the usage of radioimmunoassay technique in Oncology are the major factors influencing this market. (pitchengine.com)
  • Europe was the second largest market for radioimmunoassay in 2019 due to the surge in usage of radioimmunoassay products in research laboratories, pharma-companies, and other laboratories along with rising pharmaceutical and biotechnological companies in Europe mainly in the U.K., Germany, France, and Spain. (transparencymarketresearch.com)
  • In the group of 30 patients with Addison's disease 19 had positive results in adrenal antibody radioimmunoassay (RIA). (biomedsearch.com)
  • Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis. (asm.org)
  • Detection of staphylococcal enterotoxins by enzyme-linked immunosorbent assays and radioimmunoassays: comparison of monoclonal and polyclonal antibody systems. (asm.org)
  • Goat Antibody Digoxin (125-I) Radioimmunoassay,2nd Antibody Sep. (medicregister.com)
  • In a radioimmunoassay, peptides that extend toward the amino terminal from Ala34, such as [Tyr,Gly]-ACTH34-39, ACTH18-39, and ACTH, had greater affinity for the antibody, which suggests that the antiserum recognizes the peptide bond preceding the alanyl residue. (mysciencework.com)
  • We have developed a rapid and sensitive micro-radioimmunoassay for the detection of cellular cAMP. (biomedsearch.com)
  • Detection of viral hepatitis B markers by radioimmunoassay in medical personnel. (osti.gov)
  • Marievskij, V F, and Lejbenzon, A S. Detection of viral hepatitis B markers by radioimmunoassay in medical personnel. (osti.gov)
  • Radioimmunoassay methods are used in several fields of disease diagnosis such as in early detection and diagnosis of cancer, other chronic diseases, and diagnosis of neurodegenerative disorders. (coherentmarketinsights.com)
  • There are two types of radioimmunoassay, which serve different functions for clinical diagnostics and research purposes. (marketsandmarkets.com)
  • Radioimmunoassay has applications in the field of clinical diagnostics, and is used in hospitals, contract research organizations (CRO), laboratories, pharmaceutical companies, and others. (pitchengine.com)
  • The radioimmunoassay of steroid glucuronides. (portlandpress.com)
  • The 3 alpha,17,21-P-5 beta radioimmunoassay also identified 3 alpha,17 alpha-dihydroxy-5 beta-pregnane-20-dione in all three fluids, despite the fact that this steroid was not among the radioactive incubation products of the ovary. (ualg.pt)
  • Results of radioimmunoassay procedures applied to samples containing thrombospondin indicated that reliable values are obtained either in saline or in plasma. (bloodjournal.org)
  • Caffeine in plasma and saliva by a radioimmunoassay procedure. (aspetjournals.org)
  • A homologous salmonid leptin radioimmunoassay indicates elevated plasma leptin levels during fasting of rainbow trout. (semanticscholar.org)
  • Edquist, L. E., Johansson, E. D.: Radioimmunoassay of oestrone and oestradiol in human and bovine peripheral plasma. (springer.com)
  • Radioimmunoassay of interleukin-6 in plasma. (aaccjnls.org)
  • Determination of tissue and plasma concentrations of PTHrP in fish: development and validation of a radioimmunoassay using a teleost 1-34 N-terminal peptide. (ualg.pt)
  • A simple non-chromatographic radioimmunoassay for plasma androstenedione. (semanticscholar.org)
  • Direct radioimmunoassay for melatonin in plasma. (ox.ac.uk)
  • Radioimmunoassay for prostatic acid phosphatase. (elsevier.com)
  • By the 1970s, there were already many examples of how radioimmunoassays improved diagnostic laboratory methods. (springer.com)
  • This paper describes a rapid sensitive solid phase radioimmunoassay procedure for staphylococcal enterotoxin B, which can be applied to A and C. The procedure is 100-fold more sensitive thandescribed immunodiffusion and precipitin methods and is reliable at a level of 0.01 µg/ml. (jimmunol.org)
  • Speech very, organize that each ebook methods of hormone radioimmunoassay has an homepage and, not, the spell is Similarly social from the evolution or it relates not. (wldhair.com)
  • Hawaii Pics Promethian Magos: This ebook methods of hormone radioimmunoassay has a age of advanced control. (wldhair.com)
  • The study begins with a detailed overview of the market for Europe radioimmunoassay market report 2017, including the definition, classification, and industry chain structure of Europe radioimmunoassay market report 2017, and move forward to cover every aspect of this market, counting several criteria based on which the market is classified. (qyresearchreports.com)
  • Disclaimer: Radioimmunoassay definition / meaning should not be considered complete, up to date, and is not intended to be used in place of a visit, consultation, or advice of a legal, medical, or any other professional. (definithing.com)
  • Modification of the choriogonadotropin beta-subunit radioimmunoassay for determination of urinary choriogonadotropin. (aaccjnls.org)
  • Radioimmunoassay determination of the absolute oral bioavailabilities and O-demethylation of codeine and hydrocodone in the dog. (aspetjournals.org)
  • The determination of aldolase A, B and C by radioimmunoassay may be a valuable tool in biochemical and clinical studies of aldolase isozymes. (gallusimmunotech.com)
  • CSA were quantified by using a solid phase sandwich radioimmunoassay, which was shown to be sensitive, reproducible, and S. mansoni -specific. (ajtmh.org)
  • Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. (lu.se)
  • The quantitation by radioimmunoassay of 2'-deoxyuridine 5'-triphosphate in extracts of thymidylate synthase-inhibited cells. (ox.ac.uk)
  • Erythropoietin in renal transplant recipients: studies based on recombinant human erythropoietin radioimmunoassay. (nih.gov)
  • The purification of human erythropoietin (EP) had provided small quantities of highly bioactive EP (approximately 70,000 U/mg) required for the development of an EP radioimmunoassay (RIA). (nih.gov)
  • The development of a 24 hour radioimmunoassay for erythropoietin (EPO) using EPO derived from recombinant DNA as both immunogen and ligand is described in the present paper. (elsevier.com)
  • been significantly increased by a radioimmunoassay (RIA) procedure. (britannica.com)
  • Circulating thyroglobulin was measured in 20 dogs with thyroid cancer, using a homologous polyclonal radioimmunoassay. (avmi.net)
  • awarded for her development of radioimmunoassay (RIA), an extremely sensitive technique for measuring minute quantities of biologically active substances. (britannica.com)
  • A sensitive, specific and reproducible radioimmunoassay for secretin is described. (garvan.org.au)
  • Radioimmunoassay (RIA) is a sensitive method for measuring very small amounts of a substance in the blood. (discoveriesinmedicine.com)
  • A specific and sensitive radioimmunoassay (RIA) for the N-terminus of sea bream (Sparus auratus) and flounder (Platichthys flesus) parathyroid hormone-related protein (PTHrP) was developed. (ualg.pt)
  • Short shelf-life of radiolabeled compounds, hazardous radioactive elements present in the assay, and the disposal of radioactive waste are other factors negatively affecting the radioimmunoassay market. (transparencymarketresearch.com)
  • The Asian radioimmunoassay market that is expected to reach $114.8 million by 2020. (pitchengine.com)
  • Geographically, the Asian Radioimmunoassay market is expected to grow at a CAGR of 4.5% from 2015 to 2020. (pitchengine.com)
  • In terms of high growth potential, Asia Radioimmunoassay market is an open market, and expected to witness growth at the fastest rate from 2015 to 2020. (pitchengine.com)
  • This can be attributed to the by increase in Aging population, rise in outsourcing of Radioimmunoassay and increase in Research of Radioimmunoassay in Oncology. (pitchengine.com)
  • VWR Tube Racks for Radioimmunoassay Tubes, Polypropylene - Model 60985-250 - Case of 6 : Designed for easy pipetting and storage, these racks provide the extra tube capacity needed for RIA work. (egeneralmedical.com)
  • Enzyme (EIA) immunoassay was developed as an alternative to radioimmunoassay (RIA). (encyclopedia.com)
  • Results of revealing B markers of viral hepatitis (VHB) in medical personnel in the city of Zaporozhe by radioimmunoassay method are presented. (osti.gov)
  • Vyyavlenie markerov virusnogo gepatita B u meditsinskogo personala radioimmunologicheskim metodom} author = {Marievskij, V F, and Lejbenzon, A S} abstractNote = {Results of revealing B markers of viral hepatitis (VHB) in medical personnel in the city of Zaporozhe by radioimmunoassay method are presented. (osti.gov)
  • AG") were successfully radioiodinated with the chloramine-T method and used as ligand in the radioimmunoassay. (elsevier.com)
  • For instance, according to the 2018 American Cancer Society report, cancer was responsible for around 609,640 cancer deaths in the U.S. Moreover, the use of radioimmunoassay procedures in pharmaceutical industries, contract research organizations, and research centers is expected to drive growth of the radioimmunoassay market size over the forecast period. (coherentmarketinsights.com)
  • Furthermore, increasing demand for healthcare automation and high throughput procedures in diagnostic labs and research centers to ensure satisfaction of customers and patients with error free results are other factors that are expected to drive growth of radioimmunoassay market over the forecast period. (coherentmarketinsights.com)
  • The study by Raisz and colleagues reported in this issue (1), although based on a small number of samples, illustrates both the assets and limitations of commercially available radioimmunoassays for immunoreactive parathyroid hormone (iPTH). (annals.org)
  • Total immunoreactive insulin (IRI) is conventionally determined by radioimmunoassays. (diabetesjournals.org)
  • Development of a Radioimmunoassay for ±-Fetoprotein. (cancerquest.org)
  • In the recent times, the global market for Europe radioimmunoassay market report 2017 has surfaced as one of the most promising markets in the pharmaceutical industry, thanks to the significant rise in research and development activities by leading vendors of Europe radioimmunoassay market report 2017 across the world. (qyresearchreports.com)
  • It provides in-depth information on the development trends and the policies and regulations, concerning Europe radioimmunoassay market report 2017, implemented in each of the geographical segments. (qyresearchreports.com)
  • Development and application of a specific radioimmunoassay for trifluoperazine N4'-oxide to a kinetic study in humans. (aspetjournals.org)
  • Development and validation of a salmon growth hormone radioimmunoassay. (semanticscholar.org)
  • Identification of, and development of radioimmunoassays for 17 alpha, 21-dihydroxy-4-pregnene-3,20-dione and 3 alpha,17 alpha, 21-trihydroxy-5 beta-pregnan-20-one in the ovaries of mature plaice (Pleuronectes platessa). (ualg.pt)
  • The radioimmunoassays proved to be highly useful to trace bikunin and fetuin in the course of developing isolation procedures, since neither specific functional assays nor monospecific antisera were available. (uni-muenchen.de)
  • Subunit-specific radioimmunoassay for aldolase A, B, and C subunits: clinical significance. (gallusimmunotech.com)
  • Application of a sequence-specific radioimmunoassay for the ca. (mysciencework.com)
  • Application of a sequence-specific radioimmunoassay for the carboxyl-terminal region of corticotropin. (mysciencework.com)
  • The Asian Radioimmunoassay Market report defines and segments the concerned market with analysis and forecast of revenue. (pitchengine.com)
  • Browse through the TOC of Asian Radioimmunoassay Market for an analysis of industry trends, segments & forecasts. (pitchengine.com)
  • Moreover, these regions are sub-divided to offer an exhaustive landscape of the Radioimmunoassay Market market across key countries in respective regions. (pharmiweb.com)
  • The research report is formed by collating different statistics and information concerning the Radioimmunoassay Market market. (pharmiweb.com)
  • The global radioimmunoassay (RIA) market constituted 2.3% share of the global immunoassay market. (pitchengine.com)
  • Radioimmunoassay also has it application in the research purpose, and contributed a share of 65.9% to the overall radioimmunoassay market in 2014. (pitchengine.com)
  • Manufacturers have turned to technological innovations and data-driven customization to satisfy the augmenting consumer demand for efficiency and more accuracy in results, leading to an increased usage of technology in the Europe radioimmunoassay market report 2017 production processes, which is also reflecting positively on the growth of this market. (qyresearchreports.com)
  • The research report on the Europe radioimmunoassay market report 2017 is an analytical study which comprehensively analyzes the competitive framework of this market. (qyresearchreports.com)
  • Using a number of effective assessment tools, such as porter's five forces and value chain analysis, it performs in-depth analyses of the production and supply as well as the demand and sales of Europe radioimmunoassay market report 2017 and provides deep insights into the future prospects of this market. (qyresearchreports.com)
  • With chemical industry undergoing a phase of technological disruption, innovations in products are likely to shape the future of the Europe radioimmunoassay market report 2017. (qyresearchreports.com)
  • Further, it offers an estimation of the market size in terms of value (US$) and in volume (kilo tons) and talks about the key segments and the geographical subdivisions of the market for Europe radioimmunoassay market report 2017 in details. (qyresearchreports.com)
  • The predominant applications of the Europe radioimmunoassay market report 2017 have also been discussed at length in this research study. (qyresearchreports.com)
  • With all these analyses and information, this report can act as a valuable guide to readers looking to gain a clear understanding of all the factors that are influencing the market for Europe radioimmunoassay market report 2017 at present and are projected to remain doing so over the forecast period. (qyresearchreports.com)
  • Rise in prevalence of cancer and other infectious diseases is expected to lead to growth of the radioimmunoassay market. (transparencymarketresearch.com)
  • This is one of the factors that has resulted in growth of the radioimmunoassay market worldwide. (transparencymarketresearch.com)
  • Radioimmunoassay market is projected to witness significant growth over the forecast period, owing to rising prevalence of various infectious diseases and cancer. (coherentmarketinsights.com)
  • Moreover, government support towards the infection-free states is also increasing growth of radioimmunoassay market. (coherentmarketinsights.com)
  • The Middle East and Africa Radioimmunoassay Market was worth USD 33.21 million in 2016 and estimated to be growing at a CAGR of 3.18%, to reach USD 38.84 million by 2021. (marketdataforecast.com)
  • The major factors affecting the Middle East and Africa Radioimmunoassay Market include the high investment, the increasing emphasis on R&D in the sector, technological advancements, their increased usage in pharmaceutical industries, research, rising need for quality and error-free diagnosis and consumer awareness. (marketdataforecast.com)
  • The Middle East and Africa Radioimmunoassay Market is highly competitive with a large number of players. (marketdataforecast.com)