Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Cross Reactions
Immune Sera
Iodine Isotopes
Iodine Radioisotopes
Microchemistry
Antibody Specificity
Reagent Kits, Diagnostic
Evaluation Studies as Topic
Thyroxine
The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.
Rabbits
Biological Assay
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Chromatography, High Pressure Liquid
Digoxin
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
Cortodoxone
Reference Values
Hyperthyroidism
Thyroxine-Binding Proteins
Hypothyroidism
Immunoenzyme Techniques
Antigen-Antibody Reactions
Pregnancy
Antibodies
Luteinizing Hormone
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Luteinizing hormone regulates steroid production by the interstitial cells of the TESTIS and the OVARY. The preovulatory LUTEINIZING HORMONE surge in females induces OVULATION, and subsequent LUTEINIZATION of the follicle. LUTEINIZING HORMONE consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH and FSH), but the beta subunit is unique and confers its biological specificity.
Chromatography, Gel
Immunoglobulin G
Immunodiffusion
Antigen-Antibody Complex
Thromboxane B2
Charcoal
Estriol
A hydroxylated metabolite of ESTRADIOL or ESTRONE that has a hydroxyl group at C3, 16-alpha, and 17-beta position. Estriol is a major urinary estrogen. During PREGNANCY, a large amount of estriol is produced by the PLACENTA. Isomers with inversion of the hydroxyl group or groups are called epiestriol.
Hepatovirus
Hepatitis B Antigens
6-Ketoprostaglandin F1 alpha
Endorphins
One of the three major groups of endogenous opioid peptides. They are large peptides derived from the PRO-OPIOMELANOCORTIN precursor. The known members of this group are alpha-, beta-, and gamma-endorphin. The term endorphin is also sometimes used to refer to all opioid peptides, but the narrower sense is used here; OPIOID PEPTIDES is used for the broader group.
Immunoassay
Gastrins
Testosterone
A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.
Thyrotropin
A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.
Cattle
Immunoglobulin M
Progesterone
The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.
Pituitary Gland
Prostaglandins F
(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.
beta-Endorphin
Immunoelectrophoresis
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Triiodothyronine
A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.
17-Hydroxycorticosteroids
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Rats, Inbred Strains
Binding Sites, Antibody
Placental Lactogen
A polypeptide hormone of approximately 25 kDa that is produced by the SYNCYTIOTROPHOBLASTS of the PLACENTA, also known as chorionic somatomammotropin. It has both GROWTH HORMONE and PROLACTIN activities on growth, lactation, and luteal steroid production. In women, placental lactogen secretion begins soon after implantation and increases to 1 g or more a day in late pregnancy. Placental lactogen is also an insulin antagonist.
Immunosorbents
Estradiol
Hydrocortisone
Prostaglandins E
(11 alpha,13E,15S)-11,15-Dihydroxy-9-oxoprost-13-en-1-oic acid (PGE(1)); (5Z,11 alpha,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dien-1-oic acid (PGE(2)); and (5Z,11 alpha,13E,15S,17Z)-11,15-dihydroxy-9-oxoprosta-5,13,17-trien-1-oic acid (PGE(3)). Three of the six naturally occurring prostaglandins. They are considered primary in that no one is derived from another in living organisms. Originally isolated from sheep seminal fluid and vesicles, they are found in many organs and tissues and play a major role in mediating various physiological activities.
Binding, Competitive
Prolactin
A lactogenic hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). It is a polypeptide of approximately 23 kD. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin receptors are present in the mammary gland, hypothalamus, liver, ovary, testis, and prostate.
Prostaglandins
Chorionic Gonadotropin
A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN).
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Menstruation
Beta-Globulins
Growth Hormone
Methaqualone
Follicle Stimulating Hormone
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Follicle-stimulating hormone stimulates GAMETOGENESIS and the supporting cells such as the ovarian GRANULOSA CELLS, the testicular SERTOLI CELLS, and LEYDIG CELLS. FSH consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity.
Epoprostenol
Adrenocorticotropic Hormone
An anterior pituitary hormone that stimulates the ADRENAL CORTEX and its production of CORTICOSTEROIDS. ACTH is a 39-amino acid polypeptide of which the N-terminal 24-amino acid segment is identical in all species and contains the adrenocorticotrophic activity. Upon further tissue-specific processing, ACTH can yield ALPHA-MSH and corticotrophin-like intermediate lobe peptide (CLIP).
Cyclosporins
A group of closely related cyclic undecapeptides from the fungi Trichoderma polysporum and Cylindocarpon lucidum. They have some antineoplastic and antifungal action and significant immunosuppressive effects. Cyclosporins have been proposed as adjuvants in tissue and organ transplantation to suppress graft rejection.
Hepatitis A
Peptide Fragments
Goats
Estrone
An aromatized C18 steroid with a 3-hydroxyl group and a 17-ketone, a major mammalian estrogen. It is converted from ANDROSTENEDIONE directly, or from TESTOSTERONE via ESTRADIOL. In humans, it is produced primarily by the cyclic ovaries, PLACENTA, and the ADIPOSE TISSUE of men and postmenopausal women.
Dogs
Cholecystokinin
A peptide, of about 33 amino acids, secreted by the upper INTESTINAL MUCOSA and also found in the central nervous system. It causes gallbladder contraction, release of pancreatic exocrine (or digestive) enzymes, and affects other gastrointestinal functions. Cholecystokinin may be the mediator of satiety.
Clinical Enzyme Tests
Staphylococcal Protein A
A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function.
Hemagglutination Tests
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Oxytocin
Electrophoresis, Polyacrylamide Gel
Atrial Natriuretic Factor
Cells, Cultured
Counterimmunoelectrophoresis
Estrus
Dialysis
Secobarbital
Paraneoplastic Endocrine Syndromes
Nortriptyline
Gastrointestinal Hormones
Somatostatin
A 14-amino acid peptide named for its ability to inhibit pituitary GROWTH HORMONE release, also called somatotropin release-inhibiting factor. It is expressed in the central and peripheral nervous systems, the gut, and other organs. SRIF can also inhibit the release of THYROID-STIMULATING HORMONE; PROLACTIN; INSULIN; and GLUCAGON besides acting as a neurotransmitter and neuromodulator. In a number of species including humans, there is an additional form of somatostatin, SRIF-28 with a 14-amino acid extension at the N-terminal.
SRS-A
A group of LEUKOTRIENES; (LTC4; LTD4; and LTE4) that is the major mediator of BRONCHOCONSTRICTION; HYPERSENSITIVITY; and other allergic reactions. Earlier studies described a "slow-reacting substance of ANAPHYLAXIS" released from lung by cobra venom or after anaphylactic shock. The relationship between SRS-A leukotrienes was established by UV which showed the presence of the conjugated triene. (From Merck Index, 11th ed)
Thyrotropin-Releasing Hormone
A tripeptide that stimulates the release of THYROTROPIN and PROLACTIN. It is synthesized by the neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, TRH (was called TRF) stimulates the release of TSH and PRL from the ANTERIOR PITUITARY GLAND.
Immunoradiometric Assay
Chromatography, Gas
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
beta-Lipotropin
A 90-amino acid peptide derived from post-translational processing of pro-opiomelanocortin (POMC) in the PITUITARY GLAND and the HYPOTHALAMUS. It is the C-terminal fragment of POMC with lipid-mobilizing activities, such as LIPOLYSIS and steroidogenesis. Depending on the species and the tissue sites, beta-LPH may be further processed to yield active peptides including GAMMA-LIPOTROPIN; BETA-MSH; and ENDORPHINS.
Sheep
Hypothalamus
Hydroxyprogesterones
Autoantibodies to gastrin in patients with pernicious anaemia--a novel antibody. (1/8213)
Autoantibodies arise when there is a breakdown in immunological tolerance. Autoantibodies to parietal cells and intrinsic factor are found in autoimmune atrophic gastritis (AAG) and are associated with elevated plasma gastrin. Endogenous gastrin autoantibodies have not been described to date. The aim of this study was to investigate the occurrence of autoantibodies to gastrin. Plasma from 50,000 patients, including more than 2000 with AAG, was tested. Gastrin was measured by radioimmunoassay (RIA) in whole plasma and the presence of autoantibody determined by using a control which omitted assay antibody. The quantity and affinity of gastrin autoantibodies was assessed. Three patients had autoantibodies to gastrin. All three had AAG and pernicious anaemia (PA). The antibodies were of low titre and relatively high affinity. Free circulating plasma gastrin levels were within the normal range, but total gastrin levels were elevated. This is the first description of autoantibodies to endogenous gastrin. The incidence of antibodies to gastrin is low, they are found in association with PA, and they may lead to falsely low measurements of plasma gastrin. (+info)Immunoglobulin-specific radioimmunoprecipitation assays for quantitation of nasal secretory antibodies to hemagglutinin of type A influenza viruses. (2/8213)
Radioimmunoprecipitation (RIP) assays were developed to selectively quantitate class-specific antibodies to purified hemagglutinins (HA) of type A influenza virus in nasal secretions. Rabbit anti-human secretory piece of immunoglobulin A (IgA) and rabbit anti-human IgG were used as second antibodies. A third antibody, goat anti-rabbit IgG, was incorporated into the system to separate immune complexes formed between iodinated HA, nasal wash test specimen, and second antibody. The utilization of this reagent avoided the need for large quantities of IgA and IgG antibody-negative carrier secretions. Nasal was specimens obtained from 14 adults immunized with an inactivated type A influenza virus vaccine were evaluated by RIP and viral neutralization assays. Significant homologous postvaccination secretory IgA and IgG antibody levels were demonstrable in 13 (93%) of individuals by RIP, whereas only 5 (36%) exhibited rises by viral neutralization tests. Moreover, the geometric mean IgA and IgG antibody levels were at least 20- and 37-fold greater than the neutralizing antibody titer. The pattern of heterologous immunoglobulin-specific antibody responses tended to be similar to those observed with the homologous HA subunit. (+info)Influences of Helicobacter pylori on serum pepsinogen concentrations in dialysis patients. (3/8213)
BACKGROUND: Patients with impaired renal function have been known to have elevated concentrations of serum pepsinogens, which are raised by Helicobacter pylori infection of the stomach. The present study was performed to examine the effect of H. pylori infection on serum pepsinogen concentrations in dialysis patients. METHODS: Forty nine patients on dialysis and 48 subjects with no known kidney disease were examined for upper gastroduodenal endoscopy, H. pylori infection and serum concentrations of pepsinogen I and II. The status of H. pylori infection was evaluated from results of a urease test, histology and culture of biopsy specimens of the gastric mucosa. Serum pepsinogen levels were measured by radioimmunoassay. RESULTS: Serum concentrations of pepsinogen I and II were elevated in the dialysis patients in comparison with those in the controls (277.4+/-24.2 vs 52.6+/-4.0 pg/ml, P<0.01 for pepsinogen I, and 30.2+/-2.9 vs 14.9+/-1.3 pg/ml, P<0.01 for pepsinogen II). In both the dialysis patients and controls, those with H. pylori infection had significantly higher concentrations of serum pepsinogen I and II and a lower ratio of pepsinogen I to pepsinogen II than those without infection. Among the controls, 15 of 25 subjects with atrophic gastritis had a pepsinogen I/pepsinogen II ratio < or = 3.0, while only two out of 17 patients on dialysis fell into this range. CONCLUSIONS: We conclude that H. pylori status should be taken into account when serum pepsinogen concentrations are evaluated in dialysis patients. (+info)Pregnancy detection and the effects of age, body weight, and previous reproductive performance on pregnancy status and weaning rates of farmed fallow deer (Dama dama). (4/8213)
Fallow does (n = 502) of different ages (mature, 2-yr-old, and yearling) were maintained with bucks for a 60-d breeding season to determine whether previous reproductive performance and changes in BW affect doe pregnancy rates and to compare the effectiveness of ultrasonography and serum pregnancy-specific protein B (PSPB) for the detection of pregnancy in fallow does. Ultrasonography was performed, blood samples collected, and BW recorded at buck removal (d 0) and at 30 and 90 d after buck removal. Lactational status (lactating = WET; nonlactating = DRY) were determined from farm records taken at weaning prior to each breeding season (autumn 1990 through autumn 1994). Ultrasonography and PSPB for determining pregnancy were in agreement 93% of the time. Overall pregnancy rates did not differ (P>.10) relative to age of the doe; the combined pregnancy rate was 92%. We also determined that 82.9% of does conceived early in the breeding season and that the incidence of embryonal-fetal mortality during the first 90 d after buck removal was 2.8%. In general, mature and 2-yr-old DRY does were heavier and had lower pregnancy rates than WET does. The overall weaning rate for all does was 77.9%. Loss in the number of fawns from pregnancy detection to weaning was equivalent to 14.8% for mature does, 24.7% for 2 yr old does, and 42.5% for yearling does. These data indicate that even though pregnancy rates were relatively high, further study is needed to determine the causes associated with subsequent fawn losses, particularly among yearling does. As a production tool, lactational WET/ DRY status testing was found to be an acceptable means for determining the reproductive potential of individual does within the herd. In addition, serum PSPB may be used in place of ultrasonography for pregnancy diagnosis in fallow deer as early as d 30 after buck removal. (+info)EGF precursor mRNA and membrane-associated EGF precursor protein in rat exorbital lacrimal gland. (5/8213)
This study was designed to demonstrate the presence of epidermal growth factor (EGF) in the rat exorbital lacrimal gland. EGF precursor gene transcription was demonstrated first by RT-PCR analysis of lacrimal gland RNA using a set of specific primers and second by Northern blot analysis of rat lacrimal gland mRNA. A rabbit polyclonal antibody (rEGF2) directed against rat submaxillary gland EGF was used to detect EGF-containing proteins by RIA. Results indicate that the rat lacrimal gland does not contain detectable soluble and mature EGF but that the EGF immunoreactivity is associated with the membrane-enriched fraction. Analysis of the detergent-solubilized membrane proteins by gel filtration shows that membrane-associated EGF immunoreactivity was present as a high-molecular-mass protein. Moreover, as shown by Western blot analysis, a specific anti-rat EGF precursor antibody (ppEGF1) can immunoprecipitate a 152-kDa EGF-containing protein. Taken together, these results demonstrate for the first time both EGF precursor gene transcription and EGF precursor protein expression in a lacrimal tissue, i.e., the rat exorbital lacrimal gland. The demonstration that EGF appears to be stored only as its full-length membrane precursor may provide important information to study the regulation of its secretory process. (+info)Cardiovascular, endocrine, and renal effects of urodilatin in normal humans. (6/8213)
Effects of urodilatin (5, 10, 20, and 40 ng. kg-1. min-1) infused over 2 h on separate study days were studied in eight normal subjects with use of a randomized, double-blind protocol. All doses decreased renal plasma flow (hippurate clearance, 13-37%) and increased fractional Li+ clearance (7-22%) and urinary Na+ excretion (by 30, 76, 136, and 99% at 5, 10, 20, and 40 ng. kg-1. min-1, respectively). Glomerular filtration rate did not increase significantly with any dose. The two lowest doses decreased cardiac output (7 and 16%) and stroke volume (10 and 20%) without changing mean arterial blood pressure and heart rate. The two highest doses elicited larger decreases in stroke volume (17 and 21%) but also decreased blood pressure (6 and 14%) and increased heart rate (15 and 38%), such that cardiac output remained unchanged. Hematocrit and plasma protein concentration increased with the three highest doses. The renin-angiotensin-aldosterone system was inhibited by the three lowest doses but activated by the hypotensive dose of 40 ng. kg-1. min-1. Plasma vasopressin increased by factors of up to 5 during infusion of the three highest doses. Atrial natriuretic peptide immunoreactivity (including urodilatin) and plasma cGMP increased dose dependently. The urinary excretion rate of albumin was elevated up to 15-fold (37 +/- 17 micrograms/min). Use of a newly developed assay revealed that baseline urinary urodilatin excretion rate was low (<10 pg/min) and that fractional excretion of urodilatin remained below 0.1%. The results indicate that even moderately natriuretic doses of urodilatin exert protracted effects on systemic hemodynamic, endocrine, and renal functions, including decreases in cardiac output and renal blood flow, without changes in arterial pressure or glomerular filtration rate, and that filtered urodilatin is almost completely removed by the renal tubules. (+info)cGMP-dependent and -independent inhibition of a K+ conductance by natriuretic peptides: molecular and functional studies in human proximal tubule cells. (7/8213)
In immortalized human kidney epithelial (IHKE-1) cells derived from proximal tubules, two natriuretic peptide receptors (NPR) were identified. In addition to NPR-A, which is bound by atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and urodilatin (URO), a novel form of NPR-B that might be bound by C-type natriuretic peptide (CNP) was identified using PCR. This novel splice variant of NPR-B (NPR-Bi) was also found in human kidney. Whereas ANP, BNP, and URO increased intracellular cGMP levels in IHKE-1 cells in a concentration-dependent manner, CNP had no effect on cGMP levels. To determine the physiologic responses to these agonists in IHKE-1 cells, the membrane voltage (Vm) was monitored using the slow whole-cell patch-clamp technique. ANP (10 nM), BNP (10 nM), and URO (16 nM) depolarized these cells by 3 to 4 mV (n = 47, 7, and 16, respectively), an effect that could be mimicked by 0.1 mM 8-Br-cGMP (n = 15). The effects of ANP and 8-Br-cGMP were not additive (n = 4). CNP (10 nM) also depolarized these cells, by 3+/-1 mV (n = 28), despite the absence of an increase in cellular cGMP levels, indicating a cGMP-independent mechanism. In the presence of CNP, 8-Br-cGMP further depolarized Vm significantly, by 1.6+/-0.3 mV (n = 5). The depolarizations by ANP were completely abolished in the presence of Ba2+ (1 mM, n = 4) and thus can be related to inhibition of a K+ conductance in the luminal membrane of IHKE-1 cells. The depolarizations attributable to CNP were completely blocked when genistein (10 microM, n = 6), an inhibitor of tyrosine kinases, was present. These findings indicate that natriuretic peptides regulate electrogenic transport processes via cGMP-dependent and -independent pathways that influence the Vm of IHKE-1 cells. (+info)Mediation of humoral catecholamine secretion by the renin-angiotensin system in hypotensive rainbow trout (Oncorhynchus mykiss). (8/8213)
The individual contributions of, and potential interactions between, the renin-angiotensin system (RAS) and the humoral adrenergic stress response to blood pressure regulation were examined in rainbow trout. Intravenous injection of the smooth muscle relaxant, papaverine (10 mg/kg), elicited a transient decrease in dorsal aortic blood pressure (PDA) and systemic vascular resistance (RS), and significant increases in plasma angiotensin II (Ang II) and catecholamine concentrations. Blockade of alpha-adrenoceptors before papaverine treatment prevented PDA and RS recovery, had no effect on the increase in plasma catecholamines, and resulted in greater plasma Ang II concentrations. Administration of the angiotensin-converting enzyme inhibitor, lisinopril (10(-4) mol/kg), before papaverine treatment attenuated the increases in the plasma concentrations of Ang II, adrenaline, and noradrenaline by 90, 79, and 40%, respectively and also prevented PDA and RS recovery. By itself, lisinopril treatment caused a gradual and sustained decrease in PDA and RS, and reductions in basal plasma Ang II and adrenaline concentrations. Bolus injection of a catecholamine cocktail (4 nmol/kg noradrenaline plus 40 nmol/kg adrenaline) in the lisinopril+papaverine-treated trout, to supplement their circulating catecholamine concentrations and mimic those observed in fish treated only with papaverine, resulted in a temporary recovery in PDA and RS. These results indicate that the RAS and the acute humoral adrenergic response are both recruited during an acute hypotensive stress, and have important roles in the compensatory response to hypotension in rainbow trout. However, whereas the contribution of the RAS to PDA recovery is largely indirect and relies on an Ang II-mediated secretion of catecholamines, the contribution from the adrenergic system is direct and relies at least in part on plasma catecholamines. (+info)Human prostatic acid phosphatases. II. A double-antibody radioimmunoassay<...
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Stress- and yohimbine-induced release of cholecystokinin in the frontal cortex of the freely moving rat: prevention by diazepam...
Anti-warfarin antibody preparation and its characterization for radioimmunoassay | Meta
Radioimmunoassay for ovine, caprine and bovine prolactin in pl...
Estimation by radioimmunoassay of VH determinants (Aa1) associated with rabbit T lymphocytes - Research - Aarhus University
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Monoclonal antibodies in radioimmunoassay of alphafetoprotein. Abstr. by M Uotila, E Engvall et al.
Radioimmunoassay of protein hormones: principles and methodology.
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Pancreatic-polypeptide response to food in duodenal-ulcer patients before and after vagotomy
Radioimmunoassay Market by Application & Type - 2020 | MarketsandMarkets
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U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor α -ORCA
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Protocol for steroid radioimmunoassay of plasma
A radioimmunoassay for plasma aldosterone by immunologic purification - Fingerprint - University of Texas Southwestern...
A proteína 1 relacionada a uteroglobulina está associada a gravidade da injúria inalatória
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Radioimmunoassay: a probe for the fine structure of biologic systems | Science
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Radioimmunoassay
steps in radioimmunoassay technique Radioimmunoassay at the US National Library of Medicine Medical Subject Headings (MeSH) ... A radioimmunoassay (RIA) is an immunoassay that uses radiolabeled molecules in a stepwise formation of immune complexes. A RIA ... "Solution radioimmunoassay of proteins and peptides".{{cite web}}: CS1 maint: url-status (link) Berson, Solomon A., et al. " ... A radioallergosorbent test (RAST) is an example of radioimmunoassay. It is used to detect the causative allergen for an allergy ...
PTMS (gene)
Panneerselvam C, Caldarella J, Horecker BL (1988). "A radioimmunoassay for parathymosin". J. Immunol. Methods. 104 (1-2): 131-6 ...
Maternal physiological changes in pregnancy
Determinations were via radioimmunoassay. Levels of sex hormones and SHBG during pregnancy in women. The dashed vertical lines ... Determinations were via radioimmunoassay. Levels of progesterone and estrogen rise continually throughout pregnancy, ...
Nuggehalli Raghuveer Moudgal
182-. ISBN 978-0-323-16027-8. Bernard Jaffe (2 December 2012). Methods of Hormone Radioimmunoassay. Elsevier Science. pp. 196 ... Bernard Jaffe (2 December 2012). Methods of Hormone Radioimmunoassay. Elsevier Science. pp. 196-. ISBN 978-0-323-16126-8. " ...
Extracellular adenylate cyclase
Purification, characterization, and radioimmunoassay". J. Biol. Chem. 261 (34): 16264-9. PMID 2877986. Portal: Biology v t e ( ...
Adrenocorticotropic hormone
Yalow RS, Glick SM, Roth J, Berson SA (November 1964). "Radioimmunoassay of human plasma ACTH". The Journal of Clinical ...
Estradiol enantate
It used radioimmunoassay for the determinations, with no mention of chromatographic separation. Estradiol levels following an ... Assays were performed using radioimmunoassay. Source was Wiemeyer et al. (1986). Estradiol and prolactin levels after the most ... Assays were performed using radioimmunoassay. Source was Recio et al. (1986). Estradiol levels after a single intramuscular ... Assays were performed using radioimmunoassay. Source was Garza-Flores et al. (1989). Simplified curves of estradiol levels ...
John Buster
One of Buster's earliest research studies focused on steroid hormone radioimmunoassay (RIA). He reported an RIA for the ... Buster JE, Abraham GE: Radioimmunoassay of plasma dehydroepiandrosterone sulfate. Analyt Lett 5:543:551, 1972. Buster JE, ... Buster, John E.; Abraham, Guy E. (5 December 2006). "Radioimmunoassay of Plasma Dehydroepiandrosterone Sulfate". Analytical ... "Comparison Between Four Radioimmunoassays for Plasma Estriol". Analytical Letters. 7 (2): 119-123. doi:10.1080/ ...
Robert Roberts (cardiologist)
Roberts, R.; Sobel, B. E.; Parker, C. W. (1976-11-19). "Radioimmunoassay for creatine kinase isoenzymes". Science. 194 (4267): ... "Radioimmunoassay for creatine kinase isoenzymes". Science. 194 (4267): 855-857. doi:10.1126/science.982049. ISSN 0036-8075. ...
Estradiol benzoate
Assays were performed using radioimmunoassay. Source was Shaw et al. (1975). Estradiol levels after single intramuscular ... Assays were performed using radioimmunoassay with chromatographic separation. Source was Oriowo et al. (1980). Estradiol levels ...
Radioactive tracer
125I is frequently used in radioimmunoassays because of its relatively long half-life (59 days) and ability to be detected with ... Gilby ED, Jeffcoate SL, Edwards R (July 1973). "125-Iodine tracers for steroid radioimmunoassay". The Journal of Endocrinology ...
Maurice Stroun
Leon, S.A.; Green, A.; Yaros, M.J.; Shapiro, B. (December 1975). "Radioimmunoassay for nanogram quantities of DNA". Journal of ... Leon and his collaborators developed a radioimmunoassay for measuring nanogram quantities of nucleic acids in serum. This ...
Brian Follett
His work included the development of the first radioimmunoassay to measure bird luteinizing hormone (LH) in collaboration with ... A radioimmunoassay for avian luteinizing hormone. J. Endicronol. 52: 359-378. Web of Science, https://app.webofknowledge.com/ ...
Estrone sulfate
E1S using radioimmunoassay (RIA) have been reported to be 0.96 ± 0.11 ng/mL in men, 0.96 ± 0.17 ng/mL during the follicular ... convenient radioimmunoassay of estrone sulfate". Clin. Chem. 44 (2): 244-9. doi:10.1093/clinchem/44.2.244. PMID 9474019. Cowie ... measured by a direct radioimmunoassay, and fate of exogenously injected estrone sulfate". Horm Res. 27 (2): 61-8. doi:10.1159/ ...
2-Methoxyestriol
Fujii Y, Teranishi M, Nakada K, Yamazaki M, Kishida S, Miyabo S (September 1988). "Radioimmunoassay of 2-methoxyestriol in ...
Glucagon
This led to the development of the first radioimmunoassay for detecting glucagon, described by Roger Unger's group in 1959. A ... Lundqvist, Gudmar; Edwards, John; Wide, Leif (January 1976). "A Solid Phase Radioimmunoassay for Pancreatic Glucagon". Upsala ... when a specific radioimmunoassay was developed. Cortisol Diabetes mellitus Glucagon-like peptide-1 Glucagon-like peptide-2 ...
James J. Peters VA Medical Center
Beginning in the 1950s Rosalyn Sussman Yalow and Solomon Berson conducted research into radioimmunoassay. Their laboaratory at ... Collaborated with Solomon Berson to develop radioimmunoassay. Solomon Berson - Collaborated with Rosalyn Sussman Yalow to ... develop radioimmunoassay. Nicholas J. Cifarelli - Nephrology. Later pioneered the first Bioethics Advisory Committee in the ...
Estetrol
Kundu N, Grant M (June 1976). "Radioimmunoassay of 15alpha-hydroxyestriol (estetrol) in pregnancy serum". Steroids. 27 (6): 785 ...
Geoffrey Ling
1981). "Methadone: radioimmunoassay and pharmacokinetics in the rat". Journal of Pharmacology and Experimental Therapeutics. ...
Tiamenidine
"Determination of tiamenidine in biological specimens by radioimmunoassay". Arzneimittel-Forschung. 31 (3): 419-24. PMID 7194666 ...
Estradiol undecylate
Estradiol 17β, estrone, FSH and LH were measured in serum by radioimmunoassay before and after application of the hormone and ... Assays were performed using radioimmunoassay with chromatographic separation. Sources were Geppert (1975) and Leyendecker et al ... Assays were performed using radioimmunoassay with chromatographic separation. Sources were Geppert (1975) and Leyendecker et al ...
Interleukin 1 beta
Dinarello CA, Renfer L, Wolff SM (1977). "Human leukocytic pyrogen: purification and development of a radioimmunoassay". ...
Angela N. H. Creager
Sensitive radioimmunoassay techniques were developed for diagnostic and medical use. Natural radioisotopes were used as tracers ...
Rijk Gispen
... and vaccinia antisera by radioimmunoassay" (PDF). Bulletin of the World Health Organization. 5 (5): 613-623. PMC 2366687. PMID ...
Anti-gliadin antibodies
The measurement of AGA is done with ELISA or radioimmunoassay. Such tests measure the level of AGA relative to a standard, such ...
PTGS1
John H, Cammann K, Schlegel W (June 1998). "Development and review of radioimmunoassay of 12-S-hydroxyheptadecatrienoic acid". ...
12-Hydroxyheptadecatrienoic acid
John, H; Cammann, K; Schlegel, W (1998). "Development and review of radioimmunoassay of 12-S-hydroxyheptadecatrienoic acid". ...
ELISA
Radioimmunoassay was first described in a scientific paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960. As ... In radioimmunoassay, the radioactivity provides the signal, which indicates whether a specific antigen or antibody is present ... Before the development of the ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using ... A suitable alternative to radioimmunoassay would substitute a nonradioactive signal in place of the radioactive signal. When ...
Harvey Berger
Matsukura, A.; Nakazawa, Y.; Hama, S. (November 1989). "[A shortened method for the assay with Centocor radioimmunoassay kit ...
Anti-nRNP
Negoro N, Kanayama Y, Takeda T, Koda S, Inoue T (July 1986). "A solid-phase radioimmunoassay for the detection of nRNP immune ...
Browsing by Subject "Radioimmunoassay"
Detectable by commercial radioimmunoassay or enzyme immunoassay
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Browsing by Subject "Radioimmunoassay"
MAUDE Adverse Event Report: ROCHE DIAGNOSTICS ELECSYS TSH ASSAY RADIOIMMUNOASSAY, THYROID-STIMULATING HORMONE
JCI -
A radioimmunoassay for serum rat thyroglobulin. Physiologic and pharmacological studies.
Erowid.org: Erowid Reference 3059 : Practical Experience with a Lysergic Acid Diethylamide (LSD) Radioimmunoassay Procedure :...
Erowid.org: Erowid Reference 8016 : Determination of N,N-dimethylindolealkylamines in plasma, blood and urine extracts by...
... blood and urine extracts by radioimmunoassay and high pressure liquid chromatography J Pharmacol Exp Ther 1978 206(1):158-66 ... A sensitive radioimmunoassay for N,N-dimethylindolealkylamine derivatives has been developed. It is possible to detect 200 to ... "Determination of N,N-dimethylindolealkylamines in plasma, blood and urine extracts by radioimmunoassay and high pressure liquid ... After chromatography, individual fractions were analyzed by the radioimmunoassay. This combination of high pressure liquid ...
Radioimmunoassay of pancreozymin cholecystokinin in human serum | Garvan Institute of Medical Research
Warfarin enantiomer disposition: Determination by stereoselective radioimmunoassay<...
Warfarin enantiomer disposition: Determination by stereoselective radioimmunoassay. C. E. Cook, Noel Ballentine, T. B. Seltzman ... In a demonstration of this selective analysis, radioimmunoassays were developed for R- and S-warfarin. The 4-carboxyethyl ... In a demonstration of this selective analysis, radioimmunoassays were developed for R- and S-warfarin. The 4-carboxyethyl ... In a demonstration of this selective analysis, radioimmunoassays were developed for R- and S-warfarin. The 4-carboxyethyl ...
Direct solid-phase radioimmunoassay of cortisol</em>...
Direct solid-phase radioimmunoassay of cortisol. / Gijzen, A.H.J.. Maastricht : Rijksuniveriteit Limburg, 1977.. Research ... Direct solid-phase radioimmunoassay of cortisol. Maastricht: Rijksuniveriteit Limburg, 1977. doi: 10.26481/dis.19770520ag ... Gijzen, A. H. J. (1977). Direct solid-phase radioimmunoassay of cortisol. [Doctoral Thesis, Maastricht University]. ... Gijzen, AHJ 1977, Direct solid-phase radioimmunoassay of cortisol, Doctor of Philosophy, Maastricht University, Maastricht. ...
radioimmunoassay | Energy Probe
Serval - Identification of human synenkephalin-like immunoreactivity in phaeochromocytoma tissue using a novel carboxy-terminal...
An antiserum raised against the synthetic tyrosinylated carboxy-terminal sequence of synenkephalin (Tyr-Glu-Glu-Ser-His-Leu-Leu-Ala) has been used to chromatographically characterize the human synenkephalin-like immunoreactivity extracted from 3 adrenal medullary phaeochromocytomas. Gel filtration chromatography identified in each tumor a single peak of 8 kDa which on subsequent ion-exchange chromatography had the elution characteristics of an acidic polypeptide. These results are compatible with the human synenkephalin sequence predicted from cDNA studies, and indicate that this is the authentic peptide.
Radioimmunoassay
A radioimmunoassay measures the concentration of an antigen, or a substance that the body identifies as foreign, in the blood. ... Goldsmith, Stanley J. Radioimmunoassay: Review of Basic Principles. Seminars in Nuclear Medicine 5 (1975): 125-52.. en_US. ... During a radioimmunoassay, a researcher prepares a mixture of radioactively labeled antigens and their corresponding antibodies ... Yalow, Rosalyn S. Radioimmunoassay. Clinical Immunology Newsletter 5 (1984): 23-. en_US. ...
Staphylococcal enterotoxin B: solid-phase radioimmunoassay. - Wikidata
RADIOIMMUNOASSAY
Tagged ANTIBODY, ANTIGEN PRESENTING CELLS, ANTIGEN-ANTIBODY INTERACTION, RADIOIMMUNOASSAY3 Comments Radioimmunoassay (RIA). ... 3) RADIOIMMUNOASSAY (RIA). Radioimmunoassay is a technique used to detect the binding of antigen and antibodies in the given ... Radioimmunoassay (RIA):. Radioimmunoassay (RIA) is one of the most responsive antigen or antibody detection techniques. In 1960 ... Tag: RADIOIMMUNOASSAY. ANTIGEN - ANTIBODY INTERACTIONS. November 17, 2020. November 17, 2020. by MicroScopia IWM, posted in ...
Details for: Radioimmunoassay : › WHO HQ Library catalog
Radioimmunoassay for human health in developing countries - Publications of the IAS Fellows
IMSEAR at SEARO: Internal attenuator counting in radioimmunoassay.
Differential Effects of Bisphenol A and Diethylstilbestrol on Human, Rat and Mouse Fetal Leydig Cell Function | PLOS ONE
Testosterone Radioimmunoassay. We measured the amount of testosterone secreted into the culture medium every 24 hours by ... The daily testosterone secretion in treated and untreated samples was measured by radioimmunoassay and the values at D1 to D3 ... radioimmunoassay as previously described [47]. The limit of detection was 8 pg/100 µl. The intra-assay coefficient of variation ...
Radioimmunoassay (RIA) - Principle, Procedure, Advantages, Applications.
RIA stands for Radioimmunoassay. It is an immunological assay. It is used to determine the concentration of antigen in the ... Components used in RIA (Radioimmunoassay). TRACER. It is a labeled ligand. There are following isotopes are used in this assay ... Advantages of RIA (Radioimmunoassay). *It is an extremely sensitive assay as it can measure antigen up to picogram quantities. ... Radioimmunoassay (RIA) - Principle, Procedure, Advantages, Disadvantages, & Applications. *Post author:SAHIL BATRA. *Post ...
Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures
Radioimmunoassay (RIA) is a very sensitive in vitro assay for detecting the presence of an antigen. ... Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures Free Download: ... Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures. Sumit Thakur October 20, 2022 Radioimmunoassay PPT: ... Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures Free Download: Radioimmunoassay (RIA) is a very ...
Download Radioimmunoassay In Basic And Clinical Pharmacology
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Radioimmunoassay of gentamicin in Micromonospora medium extracts.
Quantitation of sickle cell and normal hemoglobins in amniotic fluid by radioimmunoassay<...
Quantitation of sickle cell and normal hemoglobins in amniotic fluid by radioimmunoassay. / Garver, Fred A.; Baker, Marilyn M ... Quantitation of sickle cell and normal hemoglobins in amniotic fluid by radioimmunoassay. American journal of obstetrics and ... Quantitation of sickle cell and normal hemoglobins in amniotic fluid by radioimmunoassay. In: American journal of obstetrics ... title = "Quantitation of sickle cell and normal hemoglobins in amniotic fluid by radioimmunoassay", ...
VWR® Tube Racks for Radioimmunoassay Tubes, Polypropylene | VWR
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T3 test: MedlinePlus Medical Encyclopedia
Radioimmunoassay Market Share - Social Media Platform to Share Favorite News and Videos
Effects of Bariatric Surgery on Beta-Cell Function
Rosalyn Sussm2
- Rosalyn Sussman Yalow , "The woman behind the radioimmunoassay. (smithsonianmag.com)
- Rosalyn Sussman Yalow co-developed the radioimmunoassay (RIA), a method used to measure minute biological compounds that cause immune systems to produce antibodies. (asu.edu)
Solid-phase radioimmunoassay3
- Gijzen, A.H.J.. / Direct solid-phase radioimmunoassay of cortisol . (maastrichtuniversity.nl)
- Staphylococcal enterotoxin B: solid-phase radioimmunoassay. (wikidata.org)
- Solid phase radioimmunoassay of human beta 1C globulin. (wikidata.org)
Antibody radioimmunoassay1
- A double antibody radioimmunoassay has been developed to measure thyroglobulin in rat (RTg) serum. (jci.org)
ASSAY2
- Radioimmunoassay (RIA) is a very sensitive in vitro assay for detecting the presence of an antigen. (studymafia.org)
- Sumit Thakur Uncategorized Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures Free Download: Radioimmunoassay (RIA) is a very sensitive in vitro assay for detecting the presence of an antigen. (studymafia.org)
Serum3
Enzyme2
Procedure1
- "Practical Experience with a Lysergic Acid Diethylamide (LSD) Radioimmunoassay Procedure" Clin.Chem. . (erowid.org)
Histamine1
- Histamine was measured by radioimmunoassay. (cdc.gov)
Antigen2
- A radioimmunoassay measures the concentration of an antigen, or a substance that the body identifies as foreign, in the blood. (asu.edu)
- Radioimmunoassay is a technique used to detect the binding of antigen and antibodies in the given sample. (wordpress.com)
Urine1
- This combination of high pressure liquid chromatography and radioimmunoassay has permitted the identification and quantification of these compounds in extracts of urine, plasma and whole blood from normal individuals. (erowid.org)
Principles2
Antigens1
- During a radioimmunoassay, a researcher prepares a mixture of radioactively labeled antigens and their corresponding antibodies, or blood proteins that the body produces in response to antigens. (asu.edu)
ELISA1
- These techniques were based on di- ple and quantitative postadsorption ELISA for distinguishing agnostic approaches such as radioimmunoassays and neu- between monkeypox and vaccinia infections. (cdc.gov)
Extracts1
- Radioimmunoassay of gentamicin in Micromonospora medium extracts. (growkudos.com)
Detect1
- Although radioimmunoassay has been used to detect venom in patients with snakebites , it is impractical and expensive. (medscape.com)
Collections1
- The download radioimmunoassay in basic suits taken in January each Gift and cumulated in April, to be the image collections published for the reviewsThere collections. (osimusic.com)
Human2
- Nofal, M. (1989) Radioimmunoassay for human health in developing countries IAEA Bulletin, 31 (1). (ias.ac.in)
- A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone. (semanticscholar.org)
Internal2
Demonstration1
- In a demonstration of this selective analysis, radioimmunoassays were developed for R- and S-warfarin. (elsevier.com)
Method2
- Results were consistent with those found previously by administration of individual enantiomers, thus indicating the utility of the radioimmunoassay method. (elsevier.com)
- ACR was calculated from urinary albumin and creatinine concentrations determined using radioimmunoassay and Jaffe's method, respectively.Dictionary of similar words, Different wording, Synonyms, Idioms for Antonym of does someone goodexcellent - superior, best in its class, of the highest quality, making a person shout 'Excelsior! (kps-hr-consulting.de)
Download3
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Sensitive1
- A sensitive radioimmunoassay for N,N-dimethylindolealkylamine derivatives has been developed. (erowid.org)
Test2
- The Charm II radioimmunoassay aflatoxin test for grain detects aflatoxin B1 at 5 to 40 ppb. (charm.com)
- This test is an antibody-mediated radioimmunoassay. (medscape.com)
Measures1
- We hereby aimed to summarize available evidence of the effect of the 2 most common bariatric surgery procedures, Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG), on dynamic measures of β-cell function (BCF). (medscape.com)
Research1
- In the developing world, RIA (radioimmunoassay) is used primarily for patient management, but research activity is also increasing as expertise and resources improve. (ias.ac.in)
Individual1
- After chromatography, individual fractions were analyzed by the radioimmunoassay. (erowid.org)