A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
A Rec A recombinase found in eukaryotes. Rad51 is involved in DNA REPAIR of double-strand breaks.
Proteins obtained from ESCHERICHIA COLI.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A broad category of enzymes that are involved in the process of GENETIC RECOMBINATION.
A publication issued at stated, more or less regular, intervals.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
A syndrome of defective gonadal development in phenotypic females associated with the karyotype 45,X (or 45,XO). Patients generally are of short stature with undifferentiated GONADS (streak gonads), SEXUAL INFANTILISM, HYPOGONADISM, webbing of the neck, cubitus valgus, elevated GONADOTROPINS, decreased ESTRADIOL level in blood, and CONGENITAL HEART DEFECTS. NOONAN SYNDROME (also called Pseudo-Turner Syndrome and Male Turner Syndrome) resembles this disorder; however, it occurs in males and females with a normal karyotype and is inherited as an autosomal dominant.
Activated form of factor XI. In the intrinsic pathway, Factor XI is activated to XIa by factor XIIa in the presence of cofactor HMWK; (HIGH MOLECULAR WEIGHT KININOGEN). Factor XIa then activates factor IX to factor IXa in the presence of calcium.
The phosphoprotein encoded by the BRCA1 gene (GENE, BRCA1). In normal cells the BRCA1 protein is localized in the nucleus, whereas in the majority of breast cancer cell lines and in malignant pleural effusions from breast cancer patients, it is localized mainly in the cytoplasm. (Science 1995;270(5237):713,789-91)
A tumor suppressor gene (GENES, TUMOR SUPPRESSOR) located on human CHROMOSOME 17 at locus 17q21. Mutations of this gene are associated with the formation of HEREDITARY BREAST AND OVARIAN CANCER SYNDROME. It encodes a large nuclear protein that is a component of DNA repair pathways.
A large, nuclear protein, encoded by the BRCA2 gene (GENE, BRCA2). Mutations in this gene predispose humans to breast and ovarian cancer. The BRCA2 protein is an essential component of DNA repair pathways, suppressing the formation of gross chromosomal rearrangements. (from Genes Dev. 2000;14(11):1400-6)
Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.
Enzymes that catalyze the incorporation of deoxyribonucleotides into a chain of DNA. EC 2.7.7.-.
A United States organization of distinguished scientists and engineers established for the purpose of investigating and reporting upon any subject of art or science as requested by any department of government. The National Research Council organized by NAS serves as the principal operating agency to stimulate and support research.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Recombinases that insert exogenous DNA into the host genome. Examples include proteins encoded by the POL GENE of RETROVIRIDAE and also by temperate BACTERIOPHAGES, the best known being BACTERIOPHAGE LAMBDA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A DNA-binding protein that mediates DNA REPAIR of double strand breaks, and HOMOLOGOUS RECOMBINATION.
Proteins prepared by recombinant DNA technology.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Period after successful treatment in which there is no appearance of the symptoms or effects of the disease.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Interruptions in the sugar-phosphate backbone of DNA, across both strands adjacently.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
A way of providing nursing care that is guided by the integration of the best available scientific knowledge with nursing expertise. This approach requires nurses to critically assess relevant scientific data or research evidence, and to implement high-quality interventions for their nursing practice.
Research carried out by nurses, generally in clinical settings, in the areas of clinical practice, evaluation, nursing education, nursing administration, and methodology.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
A group of peptides characterized by length of 1-2 dozen residues with a high proportion of them being non-proteinogenic, notably alpha-aminoisobutyric acid (Aib) and isovaline, and have a C-terminal amino alcohol and N terminal alkyl group. They are found in FUNGI and some are ANTI-INFECTIVE AGENTS. They form channels or pores in target organisms. The term is a contraction of peptide-Aib-alcohol.
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The B-cell leukemia/lymphoma-2 genes, responsible for blocking apoptosis in normal cells, and associated with follicular lymphoma when overexpressed. Overexpression results from the t(14;18) translocation. The human c-bcl-2 gene is located at 18q24 on the long arm of chromosome 18.
A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein.

Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination. (1/1167)

A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.  (+info)

A unique DNase activity shares the active site with ATPase activity of the RecA/Rad51 homologue (Pk-REC) from a hyperthermophilic archaeon. (2/1167)

A RecA/Rad51 homologue from Pyrococcus kodakaraensis KOD1 (Pk-REC) is the smallest protein among various RecA/Rad51 homologues. Nevertheless, Pk-Rec is a super multifunctional protein and shows a deoxyribonuclease activity. This deoxyribonuclease activity was inhibited by 3 mM or more ATP, suggesting that the catalytic centers of the ATPase and deoxyribonuclease activities are overlapped. To examine whether these two enzymatic activities share the same active site, a number of site-directed mutations were introduced into Pk-REC and the ATPase and deoxyribonuclease activities of the mutant proteins were determined. The mutant enzyme in which double mutations Lys-33 to Ala and Thr-34 to Ala were introduced, fully lost both of these activities, indicating that Lys-33 and/or Thr-34 are important for both ATPase and deoxyribonuclease activities. The mutation of Asp-112 to Ala slightly and almost equally reduced both ATPase and deoxyribonuclease activities. In addition, the mutation of Glu-54 to Gln did not seriously affect the ATPase, deoxyribonuclease, and UV tolerant activities. These results strongly suggest that the active sites of the ATPase and deoxyribonuclease activities of Pk-REC are common. It is noted that unlike Glu-96 in Escherichia coli RecA, which has been proposed to be a catalytic residue for the ATPase activity, the corresponding residual Glu-54 in Pk-REC is not involved in the catalytic function of the protein.  (+info)

Role for caspase-mediated cleavage of Rad51 in induction of apoptosis by DNA damage. (3/1167)

We report here that the Rad51 recombinase is cleaved in mammalian cells during the induction of apoptosis by ionizing radiation (IR) exposure. The results demonstrate that IR induces Rad51 cleavage by a caspase-dependent mechanism. Further support for involvement of caspases is provided by the finding that IR-induced proteolysis of Rad51 is inhibited by Ac-DEVD-CHO. In vitro studies show that Rad51 is cleaved by caspase 3 at a DVLD/N site. Stable expression of a Rad51 mutant in which the aspartic acid residues were mutated to alanines (AVLA/N) confirmed that the DVLD/N site is responsible for the cleavage of Rad51 in IR-induced apoptosis. The functional significance of Rad51 proteolysis is supported by the finding that, unlike intact Rad51, the N- and C-terminal cleavage products fail to exhibit recombinase activity. In cells, overexpression of the Rad51(D-A) mutant had no effect on activation of caspase 3 but did abrogate in part the apoptotic response to IR exposure. We conclude that proteolytic inactivation of Rad51 by a caspase-mediated mechanism contributes to the cell death response induced by DNA damage.  (+info)

Mouse Rad54 affects DNA conformation and DNA-damage-induced Rad51 foci formation. (4/1167)

Error-free repair by homologous recombination of DNA double-strand breaks induced by ionizing radiation (IR) requires the Rad52 group proteins, including Rad51 and Rad54, in the yeast Saccharomyces cerevisiae [1]. The formation of a 'joint' molecule between the damaged DNA and the homologous repair template is a key step in recombination mediated by Rad51 and stimulated by Rad54 [2] [3] [4] [5]. Mammalian homologs of Rad51 and Rad54 have been identified [2] [3] [6]. Here, we demonstrate that mouse Rad54 (mRad54) formed IR-induced nuclear foci that colocalized with mRad51. Interaction between mRad51 and mRad54 was induced by genotoxic stress, but only when lesions that required mRad54 for their repair were formed. Interestingly, mRad54 was essential for the formation of IR-induced mRad51 foci. Rad54 belongs to the SWI2/SNF2 protein family, members of which modulate protein-DNA interactions in an ATP-driven manner [7]. Results of a topological assay suggested that purified human Rad54 (hRad54) protein can unwind double-stranded (ds) DNA at the expense of ATP hydrolysis. Unwinding of the homologous repair template could promote the formation or stabilization of hRad51-mediated joint molecules. Rad54 appears to be required downstream of other Rad52 group proteins, such as Rad52 and the Rad55-Rad57 heterodimer, that assist Rad51 in interacting with the broken DNA [2] [3] [4].  (+info)

Radiation-induced assembly of Rad51 and Rad52 recombination complex requires ATM and c-Abl. (5/1167)

Cells from individuals with the recessive cancer-prone disorder ataxia telangiectasia (A-T) are hypersensitive to ionizing radiation (I-R). ATM (mutated in A-T) is a protein kinase whose activity is stimulated by I-R. c-Abl, a nonreceptor tyrosine kinase, interacts with ATM and is activated by ATM following I-R. Rad51 is a homologue of bacterial RecA protein required for DNA recombination and repair. Here we demonstrate that there is an I-R-induced Rad51 tyrosine phosphorylation, and this induction is dependent on both ATM and c-Abl. ATM, c-Abl, and Rad51 can be co-immunoprecipitated from cell extracts. Consistent with the physical interaction, c-Abl phosphorylates Rad51 in vitro and in vivo. In assays using purified components, phosphorylation of Rad51 by c-Abl enhances complex formation between Rad51 and Rad52, which cooperates with Rad51 in recombination and repair. After I-R, an increase in association between Rad51 and Rad52 occurs in wild-type cells but not in cells with mutations that compromise ATM or c-Abl. Our data suggest signaling mediated through ATM, and c-Abl is required for the correct post-translational modification of Rad51, which is critical for the assembly of Rad51 repair protein complex following I-R.  (+info)

RAD50 and RAD51 define two pathways that collaborate to maintain telomeres in the absence of telomerase. (6/1167)

Telomere length is maintained by the de novo addition of telomere repeats by telomerase, yet recombination can elongate telomeres in the absence of telomerase. When the yeast telomerase RNA component, TLC1, is deleted, telomeres shorten and most cells die. However, gene conversion mediated by the RAD52 pathway allows telomere lengthening in rare survivor cells. To further investigate the role of recombination in telomere maintenance, we assayed telomere length and the ability to generate survivors in several isogenic DNA recombination mutants, including rad50, rad51, rad52, rad54, rad57, xrs2, and mre11. The rad51, rad52, rad54, and rad57 mutations increased the rate of cell death in the absence of TLC1. In contrast, although the rad50, xrs2, and mre11 strains initially had short telomeres, double mutants with tlc1 did not affect the rate of cell death, and survivors were generated at later times than tlc1 alone. While none of the double mutants of recombination genes and tlc1 (except rad52 tlc1) blocked the ability to generate survivors, a rad50 rad51 tlc1 triple mutant did not allow the generation of survivors. Thus RAD50 and RAD51 define two separate pathways that collaborate to allow cells to survive in the absence of telomerase.  (+info)

Three-dimensional microscopy of the Rad51 recombination protein during meiotic prophase. (7/1167)

An open question in meiosis is whether the Rad51 recombination protein functions solely in meiotic recombination or whether it is also involved in the chromosome homology search. To address this question, we have performed three-dimensional high-resolution immunofluorescence microscopy to visualize native Rad51 structures in maize male meiocytes. Maize has two closely related RAD51 genes that are expressed at low levels in differentiated tissues and at higher levels in mitotic and meiotic tissues. Cells and nuclei were specially fixed and embedded in polyacrylamide to maintain both native chromosome structure and the three dimensionality of the specimens. Analysis of Rad51 in maize meiocytes revealed that when chromosomes condense during leptotene, Rad51 is diffuse within the nucleus. Rad51 foci form on the chromosomes at the beginning of zygotene and rise to approximately 500 per nucleus by mid-zygotene when chromosomes are pairing and synapsing. During chromosome pairing, we consistently found two contiguous Rad51 foci on paired chromosomes. These paired foci may identify the sites where DNA sequence homology is being compared. During pachytene, the number of Rad51 foci drops to seven to 22 per nucleus. This higher number corresponds approximately to the number of chiasmata in maize meiosis. These observations are consistent with a role for Rad51 in the homology search phase of chromosome pairing in addition to its known role in meiotic recombination.  (+info)

Sister chromatid exchanges are mediated by homologous recombination in vertebrate cells. (8/1167)

Sister chromatid exchange (SCE) frequency is a commonly used index of chromosomal stability in response to environmental or genetic mutagens. However, the mechanism generating cytologically detectable SCEs and, therefore, their prognostic value for chromosomal stability in mitotic cells remain unclear. We examined the role of the highly conserved homologous recombination (HR) pathway in SCE by measuring SCE levels in HR-defective vertebrate cells. Spontaneous and mitomycin C-induced SCE levels were significantly reduced for chicken DT40 B cells lacking the key HR genes RAD51 and RAD54 but not for nonhomologous DNA end-joining (NHEJ)-defective KU70(-/-) cells. As measured by targeted integration efficiency, reconstitution of HR activity by expression of a human RAD51 transgene restored SCE levels to normal, confirming that HR is the mechanism responsible for SCE. Our findings show that HR uses the nascent sister chromatid to repair potentially lethal DNA lesions accompanying replication, which might explain the lethality or tumorigenic potential associated with defects in HR or HR-associated proteins.  (+info)

RAD54, a significant homologous recombination proteins, is an associate from the SWI2/SNF2 category of ATPase-dependent DNA translocases. as well as the role from the RAD54 ATPase activity with this activation is definitely controversial. Its been demonstrated that RAD54 forms a co-complex with RAD51-ssDNA filaments, stabilizing the filament in a fashion that is definitely self-employed of ATP hydrolysis by RAD54 (22, 25). Nevertheless, RAD54 mutants faulty in ATP hydrolysis neglect to stimulate RAD51 DNA strand exchange, indicating that extra downstream mechanisms are essential for the activation (14, 16, 26). Its been recommended that, through the seek out homology, binding of dsDNA by RAD54 and its own ATPase-dependent translocation along the RAD51-ssDNA filament may activate DNA strand exchange by either offering rapid delivery from the inbound dsDNA for the homology sampling by RAD51 or by locally disrupting the dsDNA foundation pairs, producing them available for the homology search from ...
Coordination between DNA replication and DNA repair ensures maintenance of genome integrity, which is lost in cancer cells. Lesions and discontinuities in the DNA template undergoing replication induce replication fork stalling. Homologous recombination (HR) proteins RAD51 and BRCA1/2 play a major role in the stability of replication forks. This function appears to be distinct from the classical one performed by these proteins in HR dependent DNA Double Strand Break repair.
RAD52 homolog (S. cerevisiae), also known as RAD52, is a protein which in humans is encoded by the RAD52 gene. The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51-related DNA recombination and repair. RAD52 mediates RAD51 function in homologous recombinational repair (HRR) in both yeast Saccharomyces cerevisiae and in mammalian cells of mice and humans. However, the RAD52 protein has distinctly different functions in HRR of yeast and humans. In S. cerevisae, Rad52 protein, acting alone, facilitates the loading of Rad51 protein onto single-stranded DNA pre-coated with replication protein A in the presynaptic phase of ...
Drexel University and Georgia Institute of Technology researchers have discovered how the Rad52 protein is a crucial player in RNA-dependent DNA repair. The results of their study, published June 8 in the journal Molecular Cell, uncover a surprising function of the homologous recombination protein Rad52. They also may help to identify new therapeutic targets for cancer treatment.. Radiation and chemotherapy can cause a DNA double-strand break, one of the most harmful types of DNA damage. The process of homologous recombination - which involves the exchange of genetic information between two DNA molecules - plays an important role in DNA repair, but certain gene mutations can destabilize a genome. For example, mutations in the tumor suppressor BRCA2, which is involved in DNA repair by homologous recombination, can cause the deadliest form of breast and ovarian cancer. Alexander Mazin, a professor at Drexel Universitys College of Medicine, and Francesca Storici, an associate professor at Georgia ...
The regulation of protein function through oligomerization is a common theme in biological systems. In this work, I have focused on the effects of the oligomeric states of the human Rad52 protein on activities related to DNA binding. HsRad52, a member of the RAD52 epistasis group, is thought to play an important and as yet undefined role in homologous recombination. HsRad52 preferentially binds to ssDNA over dsDNA and stimulates HsRad51-mediated strand exchange (Benson et al., 1998). In either the presence or absence of DNA, HsRad52 has been observed to form both 10 nm ring-like structures as well as higher order oligomers consisting of multiple 10 nm rings (Van Dyck et al., 1998; Van Dyck et al., 1999). Earlier protein-protein interaction studies mapped the domain responsible for HsRad52 self-association in the N-terminus (residues 85-159) (Shen et al., 1996). Data presented here identifies a novel self-association domain in the C-terminus of HsRad52 that is responsible for the formation of higher
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
References for Abcams Recombinant Human Rad6 protein (ab117186). Please let us know if you have used this product in your publication
Active in the repair of DNA damage and in mating-type switching. Probably involved in the repair of DNA double-strands breaks. Has a role in promoting S phase completion.
The recombination protein RAD51 is an element of the meiotic recombination pathway and has been proposed to play a role in the homology search a process by which homologous chromosomes find each other before they pair in the prophase of meiosis. likely involves a step of DNA homology acknowledgement (the homology search) and a subsequent step of personal codirectional alignment of the homologous chromosomes (pairing). Because the genomes of most plants contain large fractions of repeated sequences the homology search mechanism has the difficult task of restricting pairing to truly homologous sequences and avoiding ectopic pairing relationships. In most organisms including maize and additional vegetation meiotic chromosome pairing is definitely preceded by the formation of a polarized chromosome set up with clustered telomeres (bouquet) which is definitely thought to facilitate homologous chromosome pairing (Bass et al. 2000 Niwa et al. 2000 Trelles-Sticken et al. 2000 Cowan et al. 2001 ...
Homology-directed repair of a defective glabrous gene in Arabidopsis with Cas9-based gene targeting [Florian Hahn, Marion Eisenhut, Otho Mantegazza, Andreas P.M. Weber, January 5, 2018, BioRxiv] [https://doi.org/10.1101/243675]Overview and take-home
TY - JOUR. T1 - Structural Analysis of the Human Rad51 Protein-DNA Complex Filament by Tryptophan Fluorescence Scanning Analysis. T2 - Transmission of Allosteric Effects between ATP Binding and DNA Binding. AU - Renodon-Cornière, Axelle. AU - Takizawa, Yoshimasa. AU - Conilleau, Sébastien. AU - Tran, Vinh. AU - Iwai, Shigenori. AU - Kurumizaka, Hitoshi. AU - Takahashi, Masayuki. PY - 2008/11/14. Y1 - 2008/11/14. N2 - Abstract: Human Rad51 (HsRad51) catalyzes the strand exchange reaction, a crucial step in homologous recombination, by forming a filamentous complex with DNA. The structure of this filament is modified by ATP, which is required and hydrolyzed for the reaction. We analyzed the structure and the ATP-promoted conformational change of this filament. We systematically replaced aromatic residues in the protein, one at a time, with tryptophan, a fluorescent probe, and examined its effect on the activities (DNA binding, ATPase, ATP-promoted conformational change, and strand exchange ...
BRCC5, hRAD51, HRAD51, HsRad51, HsRAD51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein ...
sundoc Migration; Titel: DSB repair by illegitimate and homologous DNA recombination in Arabidopsis thaliana, Verfasser: Kyryk, Anzhela, 2001 ; Halle, Saale : Universitäts- und Landesbibliothek ; Göttingen : Niedersächsische Staat- und Universitätsbibliothek
S-DNA (stretched DNA) is an elongated base-paired DNA conformation under high tension. Because the RecA/Rad51 family DNA recombinases form helical filaments on DNA and mediate the formation of the DNA triplex (D-loop), in which the DNA is stretched, and because the extension of these nucleoprotein filaments is similar to the extension of S-DNA, S-DNA has long been hypothesized as a possible state ...
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Meiosis-specific Recombinase Required For Repair Of Double-strand Breaks; Also Required For Pairing Between Homologous Chromosomes; Homolog Of Rad51p And The Bacterial RecA Protein; Binds SsDNA And DsDNA, Forms Helical Filaments; Stimulated By Rdh54p
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Disassembly of RecA protein subunits from a RecA filament has long been known to occur during DNA strand exchange, although its importance to this process has been controversial. An Escherichia coli RecA E38K/DeltaC17 double mutant protein displays a unique and pH-dependent mutational separation of DNA pairing and extended DNA strand exchange. Single strand DNA-dependent ATP hydrolysis is catalyzed by this mutant protein nearly normally from pH 6 to 8.5. It will also form filaments on DNA and promote DNA pairing. However, below pH 7.3, ATP hydrolysis is completely uncoupled from extended DNA strand exchange. The products of extended DNA strand exchange do not form. At the lower pH values, disassembly of RecA E38K/DeltaC17 filaments is strongly suppressed, even when homologous DNAs are paired and available for extended DNA strand exchange. Disassembly of RecA E38K/DeltaC17 filaments improves at pH 8.5, whereas complete DNA strand exchange is also restored. Under these sets of conditions, a tight ...
Exposure of Sulfolobus solfataricus to the DNA damaging agents UV-irradiation, bleomycin or mitomycin C induces cellular aggregation.[2] Other physical stressors, such as pH or temperature shift, do not induce aggregation, suggesting that induction of aggregation is caused specifically by DNA damage. Ajon et al.[3] showed that UV-induced cellular aggregation mediates chromosomal marker exchange with high frequency. Recombination rates exceeded those of uninduced cultures by up to three orders of magnitude. Frols et al.[2][4] and Ajon et al.[3] hypothesized that the UV-inducible DNA transfer process and subsequent homologous recombinational repair represents an important mechanism to maintain chromosome integrity. This response may be a primitive form of sexual interaction, similar to the more well-studied bacterial transformation that is also associated with DNA transfer between cells leading to homologous recombinational repair of DNA damage.[5] In another related species, Sulfolobus ...
Recombination facilitates DNA repair, creates diversity for evolution, and enables the production of antibodies, whereas defects in this process are linked to cancer‐prone conditions. Recombination can be subdivided into homologous recombination (HR; Liu & West, 2004) and non‐homologous end joining (NHEJ), both of which are crucial for genome stability (Shiloh, 2004; Friedberg et al, 2005a).. S.C. West (London, UK) reviewed the mechanisms of double‐strand break (DSB) repair by HR. Mutations in the breast cancer 2 (BRCA2) gene are associated with early‐onset breast cancer and are linked to defects in DSB repair by HR. The C‐terminal BRCA2 region interacts with the essential recombination protein RAD51 and contains a site (S3291) that is phosphorylated by cyclin‐dependent kinases (CDKs). When this residue is phosphorylated, it blocks C‐terminal interactions between BRCA2 and RAD51. Phosphorylation at this site is low in S‐phase when HR is active, but increases as cells progress to ...
The BRCT domains of the BRCA1 and BARD1 tumor suppressors differentially regulate homology-directed repair and stalled fork protection. Billing et al.
RAD51 recombinase, the eukaryotic homolog of RecA, is the core component in homologous recombination (HR). It performs fundamental roles such as homologous pairing and strand exchange in repairing DNA double strand breaks [1, 2]. Rad51 binds both ssDNA/dsDNA [3-5] and forms right-handed helical nucleoprotein filaments in which DNA is extended 1.5 times that of B form DNA [4, 6-8]. Such extended conformation of DNA in ssDNA-protein filaments is instrumental in facilitating homology search and strand exchange in a three stranded pairing system [8-10]. Even though the equivalent scenario of extended DNA configuration is demonstrable in dsDNA-protein filament, its relevance in HR is not fully understood. However, based on E. coli RecA system, it is surmised that unwinding associated with dsDNA-protein filament is similarly important in four-stranded pairing and strand exchange processes [11]. The first and also most recent report that describes four-stranded exchange reaction catalyzed by RAD51 ...
Human RAD51 from Invitrogen for Western Blot and Control applications. Supplied as 20 µg purified protein (1 mg/mL) in 20mM tris HCl with 100mM KCl, 10% glycerol, 1mM DTT, 0.5mM EDTA and no preservative; pH 8.
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1. RaddingCM 1981 Recombination activities of E. coli recA protein. Cell 25 3 4. 2. LusettiSLCoxMM 2002 The bacterial RecA protein and the recombinational DNA repair of stalled replication forks. Annu Rev Biochem 71 71 100. 3. CoxMM 2007 Regulation of bacterial RecA protein function. Crit Rev Biochem Mol Biol 42 41 63. 4. CoxMM 2007 Motoring along with the bacterial RecA protein. Nat Rev Mol Cell Biol 8 127 138. 5. TamasIKlassonLCanbackBNaslundAKErikssonAS 2002 50 million years of genomic stasis in endosymbiotic bacteria. Science 296 2376 2379. 6. SeitzEMBrockmanJPSandlerSJClarkAJKowalczykowskiSC 1998 RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. Genes Dev 12 1248 1253. 7. ShinoharaAOgawaHOgawaT 1992 Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell 69 457 470. 8. VamvakasSVockEHLutzWK 1997 On the role of DNA double-strand breaks in toxicity and carcinogenesis. Crit Rev Toxicol 27 155 174. 9. KhannaKKJacksonSP ...
Required for radiation resistance and meiotic viability and presumably acts in recombination and recombinational DNA repair pathways.
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Studies in the model eukaryote Saccharomyces cerevisiae have revealed that homologous recombination (HR) is an important tool for the repair of chromosomes inju...
Homologous recombination is the exchange of pieces of DNA during the formation of eggs and sperm. Recombination allows the chromosomes to shuffle their genetic material, increasing the potential of genetic diversity. Homologous recombination is also known as crossing over. ...
The RAD 51 protein, a eukaryotic homologue of Escherichia coli RecA, plays a significant role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of th
Homologous recombination is a genetic process in which two similar DNA strands exchange material. The main purpose of homologous...
Learn more about the Dna Recombination Pathway from related diseases, pathways, genes and PTMs with the Novus Bioinformatics Tool.
A DNA repair process that involves the exchange, reciprocal or nonreciprocal, of genetic material between the broken DNA molecule and a homologous region of DNA.
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Eukaryotic cells encode two homologs of Escherichia coli RecA protein, Rad51 and Dmc1, which are required for meiotic recombination. Rad51, like E.coli RecA, forms helical nucleoprotein filaments that promote joint molecule and heteroduplex DNA formation. Electron microscopy reveals that the human meiosis-specific recombinase Dmc1 forms ring structures that bind single-stranded (ss) and double-stranded (ds) DNA. The protein binds preferentially to ssDNA tails and gaps in duplex DNA. hDmc1-ssDNA complexes exhibit an irregular, often compacted structure, and promote strand-transfer reactions with homologous duplex DNA. hDmc1 binds duplex DNA with reduced affinity to form nucleoprotein complexes. In contrast to helical RecA/Rad51 filaments, however, Dmc1 filaments are composed of a linear array of stacked protein rings. Consistent with the requirement for two recombinases in meiotic recombination, hDmc1 interacts directly with hRad51.. ...
Break-induced replication (BIR) repairs one-ended double-strand breaks in DNA similar to those formed by replication collapse or telomere erosion, and it has been implicated in the initiation of genome instability in cancer and other human diseases1,2. Previous studies have defined the enzymes that are required for BIR1-5; however, understanding of initial and extended BIR synthesis, and of how the migrating D-loop proceeds through known replication roadblocks, has been precluded by technical limitations. Here we use a newly developed assay to show that BIR synthesis initiates soon after strand invasion and proceeds more slowly than S-phase replication. Without primase, leading strand synthesis is initiated efficiently, but is unable to proceed beyond 30 kilobases, suggesting that primase is needed for stabilization of the nascent leading strand. DNA synthesis can initiate in the absence of Pif1 or Pol32, but does not proceed efficiently. Interstitial telomeric DNA disrupts and terminates BIR
Homologous recombination is a process that occurs within the chromosome and which allows one piece of DNA to be exchanged for another piece. It is a cellular mechanism that is probably part of the normal process cells use to repair breaks in their chromosomes. Homologous recombination A modified version of the target gene replaces it in the chromosome. The target gene is removed and degraded. In this example, the gene is modified by insertion of an antibiotic resistance gene, which both inactivates the gene and allows efficient selection of transformed cells. requires that the pieces of DNA undergoing recombination be almost identical (homologous) in sequence. In addition, sequences on either side of the target should be identical, to promote more efficient targeting and recombination.. By constructing a sequence that is homologous to a target sequence (such as a gene), laboratory researchers can replace one of the cells own copies of a particular gene with a copy that has been altered in some ...
File:Figure2D BarlowCell.tiff First researchers needed to map early replication origins in an activated B-cell genome. To do this they performing ChIP with anti-replication protein A (RPA). They harvested fresh mouse splenic B-cells, stimulated them with LPS/IL4 to enter cell cycle. They then inhibited replication in G1/S phase using hydroxyurea (HU) allowing RPA binds to ssDNA at stalled forks. In addition to RPA, the researchers looked for the localization of recombination proteins BRCA1 and SMC5 to regions also occupied by RPA in order to identify regions in which the fork was not only stalled but had collapsed and required homologous recombination repair. From this ChIP data, they were able to identify 2204 regions were RPA, BRCA1, and SMC5 were all localized (Figure 1D). Upon further characterization of the 2204 regions, it was found that they contained shared common elements such as highly repetitive sequences (e.g. LINE L2, SINE, DNA transposon, and tRNA elements) which are known fork ...
The transcriptome is extensively and dynamically regulated by a network of RNA modifying IPI-493 factors. by RNA interference. In addition tRNA base modifications processing and regulated cleavage have been shown to alter global patterns of mRNA translation in response to cellular stress pathways. Recent studies some of which were discussed at this workshop have rekindled interest in the emerging roles of RNA modifications in health and disease. On September 10th 2014 the Division of Cancer Biology NCI sponsored a workshop to explore the role of epitranscriptomic RNA modifications and tRNA processing in cancer progression. The workshop attendees spanned a scientific range including chemists virologists and RNA and cancer biologists. The goal of the workshop was to explore the interrelationships between RNA editing epitranscriptomics and RNA processing and the enzymatic pathways that regulate these activities in cancer initiation and progression. At the conclusion of the workshop a general ...
Background Retinal ganglion cell (RGC) differentiation in vivo is definitely a highly stereotyped process, most likely resulting from the interaction of cell type-specific transcription factors and tissue-derived signaling factors. In addition, we knocked-down and and and translational initiation: and mRNA: [28] was capable to generate a well-known but fairly fragile ciliary phenotype at low dosages (3?ng/embryo) in the genetic history of our seafood lines. At somewhat higher dosages (4?ng/embryo), however, it all caused generalized embryo fatality and severe problems that were not reverted by g53 MO co-injection (Fig.?7a). Another translation-blocking MO, in this full case against [27], was not really capable to generate a proclaimed ciliary phenotype, actually at incredibly high dosages, such as 20?ng/embryo (Fig.?7b). Therefore, we determined to style two splice-blocking MOs aimed against these two genetics (and knock-down. a-c Exterior phenotype of 48 hpf embryos shot with different ...
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LOC100420930 (SWI5 recombination repair homolog (yeast) pseudogene), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
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Traditionally, recombination reactions promoted by RecA-like proteins initiate by forming a nucleoprotein filament on a single-stranded DNA (ssDNA), which then pairs with homologous double-stranded DNA (dsDNA). In this paper, we describe a novel pairing process that occurs in an unconventional manner: RecA protein polymerizes along dsDNA to form an active nucleoprotein filament that can pair and exchange strands with homologous ssDNA. Our results demonstrate that this inverse reaction is a unique, highly efficient DNA strand exchange reaction that is not due to redistribution of RecA protein from dsDNA to the homologous ssDNA partner. Finally, we demonstrate that the RecA protein-dsDNA filament can also pair and promote strand exchange with ssRNA. This inverse RNA strand exchange reaction is likely responsible for R-loop formation that is required for recombination-dependent DNA replication.
Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
The HRD score appears capable of detecting homologous recombination defects regardless of aetiology or mechanism. This score could facilitate the use of PARP inhibitors and platinum in breast, ovarian, and other cancers.
Modern light microscopical techniques were employed to follow dynamical nuclear processes during the cell cycle and during DNA-repair. Laser-UVA-microirradiation The protein Rad51 is essential for the repair of double-strand breaks (DSBs) via the conservative homologous recombination repair pathway. To test the hypothesis that Rad51 localizes to damaged sites during DSB repair, a laser-UVA-microirradiation system was established. With this system spots with sizes around 1 µm in nuclei of living cells can be irradiated with UVA-light. After sensitization of cells by incorporation of BrdU into nuclear DNA and staining with the live cell dye Hoechst 33258, the system can be used to introduce double-strand breaks and single-strand breaks in the irradiated spots. The response of Rad51 to microirradiation By use of laser-UVA microirradiation the localization of Rad51 at damaged sites containing DNA double-strand breaks could be demonstrated. The accumulation of Rad51 at microirradiated sites was ...
The Rad51 (also known as RecA) family of recombinases executes the critical step in homologous recombination: the search for homologous DNA to serve as a template during the repair of DNA double-strand breaks (DSBs). Although budding yeast Rad51 has been extensively characterized in vitro, the strin …
During BIR, the replication machinery is involved in two distinct processes: assembly and progression of BIR forks as well as re‐synthesis of resected DNA on broken chromosomes (Fig 3A). While srs2Δ cells show defects in both, we argue that the delayed progression of BIR forks has a minor effect on cell survival because by the end of the experiment the forks in srs2Δ catch up with those in SRS2 (Fig 3E, 6 h) and successfully progress almost all the way to the end of the donor chromosome (77 kb out of 94 kb to complete replication). As BIR fork migration occurs in a Rad51‐free environment, the difference between SRS2 and srs2Δ could be caused by a delayed start of BIR synthesis in srs2Δ: Rad51 brought to the newly formed D‐loops by invading 3′‐ends might affect the recruitment of PCNA to the D‐loops as it has been demonstrated in vitro (Li et al, 2013). In contrast to BIR synthesis, DNA re‐synthesis was drastically reduced in srs2Δ, with the defect becoming more pronounced with ...
During BIR, the replication machinery is involved in two distinct processes: assembly and progression of BIR forks as well as re‐synthesis of resected DNA on broken chromosomes (Fig 3A). While srs2Δ cells show defects in both, we argue that the delayed progression of BIR forks has a minor effect on cell survival because by the end of the experiment the forks in srs2Δ catch up with those in SRS2 (Fig 3E, 6 h) and successfully progress almost all the way to the end of the donor chromosome (77 kb out of 94 kb to complete replication). As BIR fork migration occurs in a Rad51‐free environment, the difference between SRS2 and srs2Δ could be caused by a delayed start of BIR synthesis in srs2Δ: Rad51 brought to the newly formed D‐loops by invading 3′‐ends might affect the recruitment of PCNA to the D‐loops as it has been demonstrated in vitro (Li et al, 2013). In contrast to BIR synthesis, DNA re‐synthesis was drastically reduced in srs2Δ, with the defect becoming more pronounced with ...
Chk1 and Chk2 are differentially involved in homologous recombination repair and cell cycle arrest in response to DNA double-strand breaks induced by camptothecins ...
Now cloning strains can be further broken down to those with their recombination proteins knocked out and those who are not. And there is a variation with some strain having more recombinations proteins knocked out. In some situations, people want the recombination proteins working, so large scale DNA manipulations can be conducted. Other times, you want to synthesis tandem repeats Mb long, or maxiprep very large plasmids, which require a very stable e coli strain ...
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Recombination Pathways Supporting the Viability of CTF4 Mutants(A-C) Tetrad dissection of ctf4Δ X sgs1 rad52Δ and ctf4Δ X sgs1 rad51Δ (A), ctf4Δ X rad59
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I think it refers to the recombination that takes place between homologous chromosomes, which will not result in gametes with different alleles for the same trait ...
TagRFP-T^SEC^3xMyc (pDD286 - annotated ApE file). TagRFP-T is an orange-red fluorescent protein that is spectrally similar to DsRed and other old-school RFPs. This construct has Lox2272 sites in place of LoxP, allowing it to be used in a background that has already been modified with one of our green vectors. ...
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... s enhance the DNA strand exchange activity of RAD51 recombinase. Depletion of polyamines sensitizes cells to genotoxic ... The effect of polyamines on RAD51 arises from their ability to enhance the capture of homologous duplex DNA and promote RAD-51- ... Polyamines appear to have an evolutionarily conserved role in regulating recombinase activity. Spermidine is synthesized from ...
... may act similarly to DNA recombinase, an activity present in Rad51 homologs. GT198 forms heterodimer with MND1 and their ... "Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase". Genes & Development. 21 (14): 1747-1757. doi: ... GT198 has been extensively shown to regulate DNA repair, to stimulate Rad51-induced DNA strand exchange. ... "The Hop2 and Mnd1 proteins act in concert with Rad51 and Dmc1 in meiotic recombination". Nature Structural & Molecular Biology ...
"Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". J. Biol. Chem. 293: 4191- ... In budding yeast, Rad51 serves as a strand exchange protein in mitosis where it is critical for the repair of DNA breaks. Rad51 ... During meiosis, the two recombinases, Rad51 and Dmc1, interact with single-stranded DNA to form specialized filaments that are ... Base triplet stepping by the Rad51/RecA family of recombinases". Science. 349 (6251): 977-81. doi:10.1126/science.aab2666. PMC ...
The Rad51 recombinase of T. thermophila is a homolog of the Escherichia coli RecA recombinase. In T. thermophila, Rad51 ... During conjugation, Rad51 is necessary for completion of meiosis. Meiosis in T. thermophila appears to employ a Mus81-dependent ... Marsh TC, Cole ES, Stuart KR, Campbell C, Romero DP (April 2000). "RAD51 is required for propagation of the germinal nucleus in ... Exposure of T. thermophila to UV light resulted in a greater than 100-fold increase in Rad51 gene expression. Treatment with ...
Mechanistically, ZGRF1 physically interacts with the RAD51 recombinase and stimulates strand exchange by RAD51-RAD54. ZGRF1 ... June 2016). Lichten M (ed.). "Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome ...
RadA recombinase), by bacteria (RecA recombinase) and by eukaryotes from yeast to humans (Rad51 and Dmc1 recombinases). Play ... "Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". J. Biol. Chem. 293 (11): ... A central intermediate step in this process is the interaction of multiple copies of a recombinase protein with single-stranded ... Recombinases employed in this process are produced by archaea ( ...
The recombination reaction is catalyzed by enzymes known as recombinases, such as RAD51. The first step in recombination is a ... A series of steps catalyzed in part by the recombinase then leads to joining of the two helices by at least one Holliday ... Vispé S, Defais M (October 1997). "Mammalian Rad51 protein: a RecA homologue with pleiotropic functions". Biochimie. 79 (9-10 ...
The meiosis-specific recombinase Dmc1 and the general recombinase Rad51 coat the single-stranded DNA to form nucleoprotein ... One such particular protein complex that is conserved between processes is RAD51, a well conserved recombinase protein that has ... The recombinases catalyze invasion of the opposite chromatid by the single-stranded DNA from one end of the break. Next, the 3 ... The Holliday junction is a tetrahedral structure which can be 'pulled' by other recombinases, moving it along the four-stranded ...
In particular, expression of the Rad51 protein (a recombinase) is increased about 15-fold by UV treatment. List of parasites ( ... The meiosis-specific recombinase, Dmc1, is required for efficient meiotic HR, and Dmc1 is expressed in E. histolytica. The ... "Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response ...
The meiosis-specific recombinase, Dmc1, is required for efficient meiotic homologous recombination, and Dmc1 is expressed in ... These genes included Spo11, Mre11, Rad50, Rad51, Rad52, Mnd1, Dmc1, Msh and Mlh. This finding suggests that the ''Acanthamoeba ...
In yeast and other eukaryotic organisms there are two recombinases required for repairing DSBs. The RAD51 protein is required ... Recombinases are key enzymes that catalyse the strand transfer step during recombination. RecA, the chief recombinase found in ... hybrid genome Four-gamete test Independent assortment Recombination frequency Recombination hotspot Site-specific recombinase ...
Rad51 recombinase and recombination mediators. Journal of Biological Chemistry. 2003 Oct 31;278(44):42729-32. Cited 433 times ... "Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination". Cell. 162 (2): 271-286. doi:10.1016 ... role of paralogues of RAD51 in promoting RAD51 filament stability Currently, the laboratory focuses on two main topics, ... "A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling". Molecular Cell. 64 (5): ...
... binds the single strand DNA and directly interacts with the recombinase RAD51 to stimulate and maintain strand invasion, ... The localization of RAD51 to the DNA double-strand break requires the formation of the BRCA1-PALB2-BRCA2 complex. PALB2 ( ...
RecA recombinase family members are found in almost all organisms with RecA in bacteria, Rad51 and DMC1 in eukaryotes, RadA in ... The RecA recombinase family contains RecA protein from bacteria, the Rad51 and Dmc1 proteins from eukaryotes, and RadA from ... The proteins of the RecA recombinase family of proteins are thought to be descended from a common ancestral recombinase. ... The RecA/Rad51/DMC1 gene family plays a central role in homologous recombination during bacterial transformation as it does ...
Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments. J. Biol. Chem. 2018. PMID ... 脱氧核糖核蛋白也参与同源重组,一种似乎是通用的DNA修復的过程。 该过程中的中间中间步骤是将重组酶(英语:Recombinase)蛋白的多个拷
RAD51 is a eukaryote gene, encoding RAD51 protein that participates in DNA damage induction and complex signal pathway of cell ... protein at Cys 319 and irreversibly loosen a protein-protein interface that is essential for filament formation and recombinase ... RI-1 inhibits the over-expression of RAD51 in cancer cells by bonding covalently to the surface of RAD51 ... Targeted drugs to RAD51 has the prospect to be a new generation of oncologic drugs,like RI-1. It is also a unique tool for ...
... binds the single strand DNA and directly interacts with the recombinase RAD51 to stimulate strand invasion, a vital step ... Chun J, Buechelmaier ES, Powell SN (January 2013). "Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1- ... with BRCA1 and RAD51". Blood. 100 (7): 2414-20. doi:10.1182/blood-2002-01-0278. PMID 12239151. S2CID 11001855. Park JY, Zhang F ...
For example, recA recombinase, that catalyses the key functions of DNA homology search and strand exchange in the bacterial ... RAD51 and DMC1). Natural transformation in bacteria, DNA transfer in archaea, and meiosis in eukaryotic microorganisms are ...
... a recombinase from Archaea homologous to the eukaryotic RAD51 protein RADA (Radius Authenticated Device Access), 3Com's ...
... rec a recombinases MeSH D08.811.739.650.500 - rad51 recombinase MeSH D08.811.739.800 - transposon resolvases MeSH D08.811. ... 739.900 - vdj recombinases MeSH D08.811.797.500 - ribonuclease p MeSH D08.811.797.750 - rna, ribosomal, self-splicing MeSH ... rec a recombinases MeSH D08.811.913.696.445.735 - rna nucleotidyltransferases MeSH D08.811.913.696.445.735.265 - dna, catalytic ... vdj recombinases MeSH D08.811.913.696.620 - phosphotransferases (alcohol group acceptor) MeSH D08.811.913.696.620.010 - ...
Esse complexo BRCA1/BARD1 interage com RAD51, importante componente do reparo do DNA pelo método de recombinação homóloga, ... aumentando a sua atividade de recombinase. Para o reparo pós replicacional do DNA temos que o BRCA1 é capaz de modular a ... Scully, R; Chen J, Plug A, Xiao Y, Weaver D, Feunteun J, Ashley T, Livingston D M (1997). «Association of BRCA1 with Rad51 in ...
During this process, Rad51 and Dmc1 recombinases bind the 3' single-strand DNA (ssDNA) tails to form helical nucleoprotein ... In humans, the protein RAD51 is central to the homologous search and formation of the D-loop. In the bacterium Escherichia coli ... the recombinases facilitate invasion of the ssDNA end into the homologous dsDNA to form a D-loop. After strand exchange, ... "Homologous genetic recombination as an intrinsic dynamic property of a DNA structure induced by RecA/Rad51-family proteins: A ...
The RadA recombinase has greater similarity to the eukaryotic Rad51 recombinase than to the bacterial RecA recombinase. RecA ... Types include: Cre recombinase Hin recombinase Tre recombinase FLP recombinase Recombinases have a central role in homologous ... Eukaryotic Rad51 and its related family members are homologous to the archaeal RadA and bacterial RecA recombinases. Rad51 is ... "Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". J. Biol. Chem. 293 (11): ...
"Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". The Journal of Biological ... Rad51 is a member of the RecA-like NTPases. In humans, RAD51 is a 339-amino acid protein that plays a major role in homologous ... RAD51 is a eukaryotic gene. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of ... During meiosis, the two recombinases, Rad51 and Dmc1, interact with single-stranded DNA to form specialized filaments that are ...
Ang reaskiyong rekombinasyon ay kinaka-katalisa ng mga ensaym na tinatawag na mga recombinase gaya ng RAD51. Ang unang hakban ... Ang sunod sunod na mga hakban na nakatalisa sa isang bahagi ng recombinase ay tumutungo naman sa pagsasama ng dalawang mga ...
... of the RAD51 nucleofilament (an intermediate in homologous recombinational repair). (RAD51 is a homologue of bacterial RecA.) ... Eukaryotic recombinases that are homologues of RecA are also widespread in eukaryotic organisms. For example, in fission yeast ... "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases". Nature. 451 (7181): 1018-21. Bibcode ...
BRCA2 also redirects RAD51 from dsDNA and prevents dissociation from ssDNA. In addition, the four paralogs of RAD51, consisting ... "Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form". Molecular Cell. ... RAD52 has been shown to interact with RAD51. The Rad52 will ease the loading of Rad51 on ssDNA by interfering with the RPA ... It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51-related DNA recombination ...
... has been shown to interact with RAD51. The RAD54B gene is somatically mutated or deleted in numerous types of cancer ... 2004). "Human meiotic recombinase Dmc1 promotes ATP-dependent homologous DNA strand exchange". Nature. 429 (6990): 433-7. doi: ... Tanaka K, Hiramoto T, Fukuda T, Miyagawa K (Sep 2000). "A novel human rad54 homologue, Rad54B, associates with Rad51". J Biol ...
In yeast and other eukaryotic organisms there are two recombinases required for repairing DSBs. The RAD51 protein is required ... Recombinases are key enzymes that catalyse the strand transfer step during recombination. RecA, the chief recombinase found in ...
... recA recombinase, that catalyses the key functions of DNA homology search and strand exchange in the bacterial sexual process ... RAD51 and DMC1). ...
The meiosis-specific recombinase Dmc1 and the general recombinase Rad51 coat the single-stranded DNA to form nucleoprotein ... One such particular protein complex that is conserved between processes is RAD51, a well conserved recombinase protein that has ... "Fission Yeast Rad51 and Dmc1, Two Efficient DNA Recombinases Forming Helical Nucleoprotein Filaments" (Free full text) ... The Holliday junction is a tetrahedral structure which can be 'pulled' by other recombinases, moving it along the four-stranded ...
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Role of human RAD51 recombinase in the cycle checkpoint and survival of a cell. ... The RAD 51 protein, a eukaryotic homologue of Escherichia coli RecA, plays a significant role in both mitotic and meiotic ... Lim, D. and Hasty, P., A Mutation in Mouse RAD51 Results in an Early Embryonic Lethal that Is Suppressed by a Mutation in p53, ... Cells with suppressed expression of Rad51 gene have altered cell cycles and accumulate in the S and G2 phases. Our findings ...
Control of the RAD51 recombinase by the BRC repeat motifs in the breast cancer susceptibility protein BRCA2 ... The interaction between the breast cancer susceptibility protein BRCA2 and the RAD51 DNA recombinase is essential for DNA ... A single point mutation within an individual BRC repeat motif impaired RAD51 binding. Further, RAD51 was unable to interact ... I defined the minimal structural determinants required for RAD51 binding by humanising a primitive RAD51 from an Archaeon ...
... *. Myun Hwa Dunlop,‡ ... RAD51-associated protein 1 (RAD51AP1) is an important HR factor that associates with and stimulates the recombinase activity of ... Although RAD51 is present in both miotic and meiotic cells, DMC1 is expressed only during meiosis (8-10). RAD51 and DMC1 have ... Homologous recombination (HR) reactions mediated by the RAD51 recombinase are essential for DNA and replication fork repair, ...
"Rad51 Recombinase" by people in this website by year, and whether "Rad51 Recombinase" was a major or minor topic of these ... "Rad51 Recombinase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... A Rec A recombinase found in eukaryotes. Rad51 is involved in DNA REPAIR of double-strand breaks. ... Below are the most recent publications written about "Rad51 Recombinase" by people in Profiles. ...
RAD51 gene. RAD51 recombinase. Enable Javascript to view the expand/collapse boxes.. Printable PDF Open All Close All ... The RAD51 protein binds to the DNA at the site of a break and encases it in a protein sheath, which is an essential first step ... The RAD51 gene provides instructions for making a protein that is essential for repairing damaged DNA. Breaks in DNA can be ... The RAD51 protein is also thought to be involved in the development of nervous system functions that control movement, but its ...
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PML-RAR oncoprotein is a blend proteins of promyelocytic leukemia (PML) and the retinoic acidity receptor- (RAR) and causes extreme promyelocytic leukemias (APL). on myeloid difference, HL-60 and NB4 cells had been incubated with an autophagy inhibitor (age.g., 3-Mother) or an autophagy inducer (age.g., rapamycin) in the existence or lack of ATRA, adopted by movement cytometric evaluation of the myeloid difference gun Compact disc11b. Co-treatment with rapamycin and ATRA for 48C72 l lead in noted induction of Compact disc11b phrase and a higher level of growth, relatives C646 to that noticed with ATRA or rapamycin only (Fig. 3A and N). In comparison, 3-Mother inhibited ATRA-induced Compact disc11b phrase and NB4 cell difference as evaluated by morphological statement (Fig. 3A and N). These data recommended that autophagy can be included in myeloid cell difference. Shape 3 Autophagy manages ATRA-induced cell difference. (A) HL-60 and NB4 cells had been treated with ATRA (1 Meters) with or without ...
Recombinase. UvsX. RecA. Rad51 Recombinase regulators. ?. ?. Rad55/57 Auxiliary helicases. UvsW. RecG. Rad54/Tid1 ...
RAD51: RAD51 recombinase. *RAF1: Raf-1 proto-oncogene, serine/threonine kinase. *RAG1: recombination activating 1 ...
The RAD51 gene provides instructions for making a protein that is essential for repairing damaged DNA. Breaks in DNA can be ... URL of this page: https://medlineplus.gov/genetics/gene/rad51/ RAD51 gene. RAD51 recombinase ... The RAD51 protein binds to the DNA at the site of a break and encases it in a protein sheath, which is an essential first step ... The RAD51 gene provides instructions for making a protein that is essential for repairing damaged DNA. Breaks in DNA can be ...
RAD51. 15q15.1. DNA recombinase. Fork stabilization and HR. Regulation of axonal branching through Netrin-1 modulation. ... Eventually, when activated by BRCA2 and PALB2, the recombinase RAD51 assembles a single-stranded nucleoprotein filament (ssDNA ... RAD51 paralog. HR. -. Ovarian cancer. FANCP. SLX4. 16p13.3. Multidomain scaffold protein. Nuclease scaffold for ICL unhooking. ... F) Next, the ssDNA overhang acts as a template for the homologous recombination mediated by RAD51/BRCA2/PALB2/BRCA1 proteins. ( ...
2021 Identification of specific inhibitors of human RAD51 recombinase. Theme by Anders Norén - Up ↑ ...
One of the critical genes for DNA repair and meiotic recombination in yeast is RAD51, and homologs of RAD51 have been ... Rad51 Recombinase * Rec A Recombinases / genetics* * Sequence Homology, Amino Acid * Tissue Distribution ... One of the critical genes for DNA repair and meiotic recombination in yeast is RAD51, and homologs of RAD51 have been ... Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene Genomics. 1998 ...
Recent advances using high-resolution microscopy and single molecule techniques have broadened our knowledge of Rad51 filament ... Rad51 Recombinase Grant support * R01 ES024872/ES/NIEHS NIH HHS/United States ... Keywords: DNA replication; RAD51; Rad51 paralogues; Shu complex; complexe Shu; homologous recombination; paralogues de Rad51; ... Rad51 filament formation and strand invasion is regulated by many mediator proteins such as the Rad51 paralogues and the Shu ...
... shown that a 28-amino acid peptide derived from the BRC4 motif of BRCA2 tumor suppressor inhibits selectively human RAD51 ... recombinase (HsRad51). With the aim of designing better inhibitors for cancer treatment, we combined an in silico docking ... Design of Potent Inhibitors of Human RAD51 Recombinase Based on BRC Motifs of BRCA2 Protein: Modeling and Experimental ... that a 28-amino acid peptide derived from the BRC4 motif of BRCA2 tumor suppressor inhibits selectively human RAD51 recombinase ...
These results highlight the importance of Rad51 interaction in the anti-recombinase function of Srs2, and provide evidence that ... An internal deletion in Srs2 likewise diminishes Rad51 interaction and anti-recombinase activity. We also present evidence that ... Rad51 presynaptic filament disruption as measured by a topoisomerase-linked assay. (A) Reaction scheme for detecting Rad51 ... Two-hybrid interactions of Rad51 with Srs2. (A) Fragments of Srs2 were tested for two-hybrid interaction with Rad51 by plating ...
RAD51, BRCC5, FANCR, HHsRad51, HsT16930, MRMV2, RAD51A, RECA, RAD51 recombinase. External IDs. OMIM: 179617 MGI: 97890 ... RAD51 expression in cancer[edit]. In eukaryotes, RAD51 protein has a central role in homologous recombinational repair. RAD51 ... "Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". J. Biol. Chem. doi:10.1074 ... Rad51 is a member of the RecA-like NTPases.. Function[edit]. In humans, RAD51 is a 339-amino acid protein that plays a major ...
The RAD51 protein is required for meiotic and mit ... EC 2.7.7.-/RAD51 protein, human; EC 2.7.7.-/Rad51 Recombinase ... Rad51 Recombinase / genetics*. Treatment Outcome. Uterine Cervical Neoplasms / drug therapy, radiotherapy, therapy*. ... Our results indicate an influence of the RAD51 genetic variants in overall survival of cervical cancer. Thereby, RAD51 G172T ... The RAD51 protein is required for meiotic and mitotic recombination and plays a central role in homology-dependent ...
Buy our Recombinant Human Rad51 protein. Ab81943 is a full length protein produced in Escherichia coli and has been validated ... RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae). *RAD51 homolog A. *RAD51 recombinase ... Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers ... Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its ...
The RAD51B protein is one of five RAD51 paralogs that function in the homologous recombinational repair pathway in higher ... The RAD51 protein is a central player in homologous recombinational repair. ... Rad51 Recombinase ... The RAD51 protein is a central player in homologous ... unexpectedly binds to the RAD51 and RAD51B proteins and stimulates the RAD51-mediated homologous pairing and strand exchange. ...
DNA recombinase assembly Source: UniProtKB. *DNA repair Source: UniProtKB ,p>Traceable Author Statement,/p> ,p>Used for ... sp,Q08297,RAD51_MOUSE DNA repair protein RAD51 homolog 1 OS=Mus musculus OX=10090 GN=Rad51 PE=1 SV=1 ... cd01123 Rad51_DMC1_radA, 1 hit. InterProi. View protein in InterPro. IPR003593 AAA+_ATPase. IPR011941 DNA_recomb/repair_Rad51 ... IPR010995 DNA_repair_Rad51/TF_NusA_a-hlx. IPR027417 P-loop_NTPase. IPR033925 Rad51_DMC1_RadA. IPR020588 RecA_ATP-bd. IPR020587 ...
RAD51 protein, S cerevisiae); EC 2.7.7.- (Rad51 Recombinase); EC 3.1.- (Exodeoxyribonucleases); EC 3.1.11.1 ( ... Rad51 Recombinase/metabolismo. Reparo de DNA por Recombina o. Prote nas de Saccharomyces cerevisiae/metabolismo. Saccharomyces ... The Rad51 (also known as RecA) family of recombinases executes the critical step in homologous recombination: the search for ... neither Rad51 recombinase nor the yeast Rad52 paralog Rad59 has this activity. In accord with our in vitro results, our ...
RAD51 RAD51 Recombinase. Protein Coding. 53. GC15P040694. 0.81. 3. LYN LYN Proto-Oncogene, Src Family Tyrosine Kinase. Protein ...
Rad51 Recombinase/antagonistas & inibidores. Carcinoma de Pequenas C lulas do Pulm o/tratamento farmacol gico. ... Rad51 Recombinase/uso terap utico. Carcinoma de Pequenas C lulas do Pulm o/gen tica. ...
Mouse monoclonal Rad51 antibody [14B4] validated for WB, IP, IHC and tested in Human. Referenced in 52 publications and 9 ... RAD51 homolog antibody. *RAD51 recombinase antibody. *RAD51, S. cerevisiae, homolog of antibody ... Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + Jurkat whole cell extract at 30 µg. Developed using the ECL technique. ... Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg. Secondary. HRP ...
Flott, S., Kwon, Y., Pigli, Y.Z., Rice, P.A., Sung, P., and Jackson, S.P. (2011) Regulation of Rad51 function by ... subtilis serine/threonine protein kinase YabT is involved in spore development via phosphorylation of a bacterial recombinase. ... 1998) Regulation of Rad51 function by c-Abl in response to DNA damage. J Biol Chem 273: 3799-3802.. *CrossRef, ... 2009) c-ABL tyrosine kinase stabilizes Rad51 chromatin association. Biochem Biophys Res Commun 382: 286-291.. *CrossRef, ...
RAD51. RAD51 recombinase. RAD51. 147. RAD52. RAD52 homolog, DNA repair protein. RAD52. ...
RAD51 Recombinase. Mediates homologous recombination and DNA damage repair. ORF. Open reading frame, or predicted coding ...
Swi5-Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation Chih-Hao Lu, Hsin-Yi Yeh, Guan-Chin ... Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase J. Brooks Crickard, Kyle Kaniecki, Youngho ...
  • The interaction between the breast cancer susceptibility protein BRCA2 and the RAD51 DNA recombinase is essential for DNA repair via homologous recombination. (bl.uk)
  • Homologous recombination (HR) reactions mediated by the RAD51 recombinase are essential for DNA and replication fork repair, genome stability, and tumor suppression. (pubmedcentralcanada.ca)
  • These single-stranded DNA tails are coated by either RAD51 or DMC1 recombinase to form a helical nucleoprotein filament, termed the presynaptic filament, that mediates DNA strand exchange with a homologous donor ( 6 ). (pubmedcentralcanada.ca)
  • In this review we focus on new insights that challenge our understanding of homologous recombination (HR) and Rad51 regulation. (nih.gov)
  • RAD51 family members are homologous to the bacterial RecA , Archaeal RadA and yeast Rad51. (wikipedia.org)
  • In humans, RAD51 is a 339- amino acid protein that plays a major role in homologous recombination of DNA during double strand break repair. (wikipedia.org)
  • In eukaryotes, RAD51 protein has a central role in homologous recombinational repair. (wikipedia.org)
  • RAD51 catalyses strand transfer between a broken sequence and its undamaged homologue to allow re-synthesis of the damaged region (see homologous recombination models ). (wikipedia.org)
  • The RAD51 protein is a central player in homologous recombinational repair. (biomedsearch.com)
  • The RAD51B protein is one of five RAD51 paralogs that function in the homologous recombinational repair pathway in higher eukaryotes. (biomedsearch.com)
  • In the present study, we found that the human EVL (Ena/Vasp-like) protein, which is suggested to be involved in actin-remodeling processes, unexpectedly binds to the RAD51 and RAD51B proteins and stimulates the RAD51-mediated homologous pairing and strand exchange. (biomedsearch.com)
  • Types include: Cre recombinase Hin recombinase Tre recombinase FLP recombinase Recombinases have a central role in homologous recombination in a wide range of organisms. (wikipedia.org)
  • During meiosis Rad51 interacts with another recombinase, Dmc1, to form a presynaptic filament that is an intermediate in homologous recombination. (wikipedia.org)
  • Like Rad51, Dmc1 is homologous to bacterial RecA. (wikipedia.org)
  • Some DNA viruses encode a recombinase that facilitates homologous recombination. (wikipedia.org)
  • The effect of polyamines on RAD51 arises from their ability to enhance the capture of homologous duplex DNA and promote RAD-51-mediated homologous DNA pairing and exchange activity. (wikipedia.org)
  • Fork reversal was found to be dependent on the central homologous recombination factor RAD51, which is consistently present at replication forks independently of their breakage, and to be antagonized by poly (ADP-ribose) polymerase/RECQ1-regulated restart. (rupress.org)
  • RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. (avivasysbio.com)
  • Our findings suggest that the Sgs1 - Rad51 interaction stimulates homologous recombination (HR). However, unlike sgs1 mutations, which impair the resection of DNA double-strand ends, negative genetic interactions of the sgs1 -FD allele are not suppressed by YKU70 deletion. (genetics.org)
  • Previous studies have shown that overexpression of RAD51 in different organisms and cell types leads to a wide range of consequences, including increased homologous recombination and increased resistance to DNA damage (2). (novusbio.com)
  • DNA repair protein RAD51 homolog 1 (RAD51) plays a central role in homologous recombination (HR) repair of DNA breaks. (go.jp)
  • In homologous recombination-dependent DSB repair, nucleases initiate homologous recombination by processing DSBs to generate 3′ overhangs, followed by the polymerization of RAD51 recombinase on the overhang ( 9-11 ). (aacrjournals.org)
  • The recombination protein RAD51 is a component of the meiotic recombination pathway and has been proposed to play a role in the homology search, a process by which homologous chromosomes find each other before they pair in the prophase of meiosis. (plantcell.org)
  • Furthermore, when overexpressed in human cells, RAD51 significantly increases the efficiency of gene targeting, which highlights its ability to find homologous DNA sequences ( Yanez and Porter, 1999 ). (plantcell.org)
  • While yeast Rad51, Rad52, and Rad54 contribute to homologous DNA recombination (HR) to similar extents, vertebrate Rad51 plays a dominant role in HR ( 25 , 48 , 57 ). (asm.org)
  • Lee, Hyunsook 2007-11-03 00:00:00 BRCA2 is involved in double-stranded DNA break repair by binding and regulating Rad51-mediated homologous recombination. (deepdyve.com)
  • Each gene product interacts with the Rad51 recombinase, a protein with a central role in homologous recombination. (pewtrusts.org)
  • In many organisms, including yeast and mammals, these single stranded ends are bound by two conserved RecA-like recombinases, Rad51 and Dmc1, to form nucleoprotein filaments that mediate strand invasion of homologous DNA duplexes [4] , [5] . (prolekare.cz)
  • Both BRCA gene products are reported to interact with the RAD51 protein, which is essential for DNA repair through homologous recombination. (news-medical.net)
  • RAD51 is a key component to the homologous recombination mechanism because it promotes homologous pairing and strand exchange. (news-medical.net)
  • Gene products of BRCA2 directly mediate the recruitment of recombinase RAD51 to double-stranded breaks during homologous recombination. (news-medical.net)
  • The interaction is an important mechanism for promoting the assembly of the synaptic complex, thereby enhancing RAD51-mediated DNA joint formation during homologous recombination. (news-medical.net)
  • This complex stimulates both synaptic and presynaptic critical steps in RAD51 and DMC1-promoted homologous pairing. (nih.gov)
  • Moreover, NS forms a DSB-induced focus and interacts with RAD51, the key enzyme of homologous recombination-mediated DSB repair pathway. (ncl.edu.tw)
  • Homology search and strand exchange mediated by Rad51 nucleoprotein filaments are key steps of the homologous recombination process. (elifesciences.org)
  • In eukaryotes, the recombinase RAD51 forms a nucleoprotein filament on ssDNA that performs synapsis and strand invasion of the homologous duplex DNA to form a stable D-loop. (elifesciences.org)
  • Kawabata M, Kawabata T, Nishibori M. Role of recA/RAD51 family proteins in mammals. (nih.gov)
  • Rad51 is a member of the RecA-like NTPases . (wikipedia.org)
  • Unlike other proteins involved in DNA metabolism, the RecA/Rad51 family forms a helical nucleoprotein filament on DNA. (wikipedia.org)
  • The RadA recombinase has greater similarity to the eukaryotic Rad51 recombinase than to the bacterial RecA recombinase. (wikipedia.org)
  • RecA recombinase appears to be universally present in bacteria. (wikipedia.org)
  • Human RAD51 family members are highly similar to bacterial RecA and yeast Rad51, both biochemically and structurally. (novusbio.com)
  • Rad51, the eukaryotic homologue of the bacterial RecA protein, plays a crucial role in these processes. (msu.ru)
  • A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes // Proceedings of the National Academy of Sciences of the USA. (msu.ru)
  • RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis. (semanticscholar.org)
  • Dmc1 and Rad51, yeast homologs of the E. coli RecA protein, are shown by immunostaining to localize to as many as 64 sites within spread meiotic nuclei. (semanticscholar.org)
  • Recombinases of the highly conserved RecA/Rad51 family form presynaptic filaments on the single-stranded DNA that is generated during early stages of recombination and DNA repair processes. (uvm.edu)
  • To better understand presynaptic filament dynamics we are studying the UvsX recombinase of bacteriophage T4 and the RecA protein of Deinococcus radiodurans, using a variety of pre-steady-state, steady-state, and single-molecule methods to determine how these enzymes recognize and process their DNA and nucleotide substrates. (uvm.edu)
  • The recombinases (RecA/Rad51), helicases, and translocases bind ssDNA to function as motor proteins. (biosyn.com)
  • Notably, we showed that the physical interaction is a species-specific since no interaction has been detected between mammalian NS and prokaryotic RecA recombinase. (ncl.edu.tw)
  • Surface Plasmon Resonance (SPR) technology supported by cell biology was employed to study the characteristics of RAD51 binding to each of the BRC repeat motifs, both independently and collectively within the context of the BRCA2 protein. (bl.uk)
  • RAD51-associated protein 1 (RAD51AP1) is an important HR factor that associates with and stimulates the recombinase activity of RAD51. (pubmedcentralcanada.ca)
  • Moreover, the meiotic activity of Rad51 appears to be restrained by the Hed1 protein ( 17 , 18 ), by the synaptonemal complex proteins Red1 and Hop1 ( 16 ), and by the Mek1-mediated phosphorylation of a key accessory factor Rad54 ( 19 ). (pubmedcentralcanada.ca)
  • The RAD51 gene provides instructions for making a protein that is essential for repairing damaged DNA. (nih.gov)
  • The RAD51 protein binds to the DNA at the site of a break and encases it in a protein sheath, which is an essential first step in the repair process. (nih.gov)
  • In the nucleus of many types of normal cells, the RAD51 protein interacts with many other proteins, including BRCA1 and BRCA2, to fix damaged DNA. (nih.gov)
  • The BRCA2 protein regulates the activity of the RAD51 protein by transporting it to sites of DNA damage in the nucleus. (nih.gov)
  • The interaction between the BRCA1 protein and the RAD51 protein is less clear, although research suggests that BRCA1 may also activate RAD51 in response to DNA damage. (nih.gov)
  • The RAD51 protein is also thought to be involved in the development of nervous system functions that control movement, but its role in this development is unclear. (nih.gov)
  • These mutations change single protein building blocks (amino acids) in the RAD51 protein sequence, or introduce a premature stop signal in the instructions for making the protein, resulting in an impaired or missing protein. (nih.gov)
  • It is unknown how this shortage of functional RAD51 protein affects nervous system development and results in the signs and symptoms of congenital mirror movement disorder. (nih.gov)
  • Purified Srs2, srs2 1-998, srs2 1-910, srs2 1-890, srs2 1-875 and srs2 1-860 were mixed with Affi-Rad51 beads ( A ) or Affi-BSA beads ( B ) The supernatant that contained unbound protein (S), wash (W) and the SDS eluate (E) were resolved by SDS-PAGE and stained with Coomassie Blue. (nih.gov)
  • The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks . (wikipedia.org)
  • The RAD51 protein is required for meiotic and mitotic recombination and plays a central role in homology-dependent recombinational repair of double-strand breaks (DSBs). (biomedsearch.com)
  • Recombination activator function of the novel RAD51- and RAD51B-binding protein, human EVL. (biomedsearch.com)
  • RAD51 is a member of the RAD51 protein family. (avivasysbio.com)
  • Transcript variants utilizing alternative polyA signals exist.The protein encoded by this gene is a member of the RAD51 protein family. (avivasysbio.com)
  • We propose that the Sgs1 - Rad51 interaction stimulates HR by facilitating the formation of the presynaptic Rad51 filament, possibly by Sgs1 competing with single-stranded DNA for replication protein A binding during resection. (genetics.org)
  • One such particular protein complex that is conserved between processes is RAD51 , a well conserved recombinase protein that has been shown to be crucial in DNA repair as well as cross over. (wikipedia.org)
  • RAD51 is a recombinase protein encoded by RAD51 gene in humans. (novusbio.com)
  • One of the major causes of drug resistance is that Gemcitabine increases the expression of RAD51 by increasing its mRNA and protein stability (4). (novusbio.com)
  • DMC1 (DNA Meiotic Recombinase 1) is a Protein Coding gene. (genecards.org)
  • Cortez, David 2018-05-28 00:00:00 Replication protein A (RPA) and RAD51 are DNA-binding proteins that help maintain genome stability during DNA replication. (deepdyve.com)
  • However, the large 400-kDa BRCA2 protein has hampered our ability to understand the entire process by which full-length BRCA2 regulates Rad51. (deepdyve.com)
  • A novel nucleic acid-binding protein that interacts with human rad51 recombinase. (wikigenes.org)
  • In the face of DNA reapplication stress, ATAD5 i) removes PCNA from the stressed DNA and simultaneously ii) recruits RAD51 recombinase through protein-protein interaction. (eurekalert.org)
  • The researchers found that ATAD5 promotes RAD51 recruitment to stalled replication sites by direct protein-protein interaction. (eurekalert.org)
  • This paradox has recently been explained by the finding that Rad51 protein, but not its strand exchange activity, promotes Dmc1 function in budding yeast. (prolekare.cz)
  • The structure of the BRCA2 protein contains a DNA binding domain that can interact with both single-stranded and double-stranded DNA, along with repeat sequences in the protein for RAD51 binding. (news-medical.net)
  • Presynaptic association of Rad51 protein with selected sites in meiotic chromatin // Proceedings of the National Academy of Sciences of USA. (msu.ru)
  • BRCA1 colocalizes with BRCA2 and Rad51 ( 10 , 41 , 52 , 70 ) and forms ionizing radiation (IR)-induced subnuclear foci (IRIF) containing Rad51 protein. (asm.org)
  • RAD51 Full-Length MS Protein Standard (NP_002866), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. (creative-proteomics.com)
  • The protein encoded by this gene is a member of the RAD51 protein family. (creative-proteomics.com)
  • Importantly, a direct protein-protein interaction between purified NS and RAD51 recombinase has been observed. (ncl.edu.tw)
  • Despite the similarities in biochemical attributes and the site of operation of RAD51 and DMC1, genetic evidence from the budding yeast suggests that the two recombinases serve distinct roles in meiotic HR ( 13 - 15 ). (pubmedcentralcanada.ca)
  • In budding yeast, Rad52 is the main mediator of Rad51 filament formation, thereby playing an essential role. (elifesciences.org)
  • We have recently shown that RAD51AP1 also partners with the meiotic recombinase DMC1, displaying isoform-specific interactions with DMC1. (pubmedcentralcanada.ca)
  • Here, we have characterized the DMC1 interaction site in RAD51AP1 by a series of truncations and point mutations to uncover a highly conserved WVPP motif critical for DMC1 interaction but dispensable for RAD51 association. (pubmedcentralcanada.ca)
  • These results further implicate RAD51AP1 in meiotic HR via RAD51 and DMC1. (pubmedcentralcanada.ca)
  • Although RAD51 is present in both miotic and meiotic cells, DMC1 is expressed only during meiosis ( 8 - 10 ). (pubmedcentralcanada.ca)
  • RAD51 and DMC1 have been shown to colocalize in side-by-side foci on meiotic chromatin, suggesting their cooperative action during meiotic HR ( 11 , 12 ). (pubmedcentralcanada.ca)
  • These and other observations have suggested that Dmc1 works in conjunction with Rad51 in forming meiotic interhomologue crossovers, whereas Rad51 acting alone may be more adept at mediating DSB repair using the sister chromatid as an information donor ( 14 ). (pubmedcentralcanada.ca)
  • In vegetative cells, Rad51 is a highly conserved recombinase that exhibits a preference for repairing double strand breaks (DSBs) using sister chromatids, in contrast to the conserved, meiosis-specific recombinase, Dmc1, which preferentially repairs programmed DSBs using homologs. (prolekare.cz)
  • Despite the different preferences for repair templates, both Rad51 and Dmc1 are required for interhomolog recombination during meiosis. (prolekare.cz)
  • Rad51 activity is inhibited in dmc1 Δ mutants, where the failure to repair meiotic DSBs triggers the meiotic recombination checkpoint, resulting in prophase arrest. (prolekare.cz)
  • The question remains whether inhibition of Rad51 activity is important during wild-type meiosis, or whether inactivation of Rad51 occurs only as a result of the absence of DMC1 or checkpoint activation. (prolekare.cz)
  • Combining dmc1-T159A with up-regulated Rad51 activity reduces interhomolog recombination and spore viability, while increasing intersister joint molecule formation. (prolekare.cz)
  • These results support the idea that down-regulation of Rad51 activity is important during meiosis to prevent Rad51 from competing with Dmc1 for repair of meiotic DSBs. (prolekare.cz)
  • The meiosis-specific recombinase Dmc1 and the general recombinase Rad51 coat the single-stranded DNA to form nucleoprotein filaments. (bionity.com)
  • Dmc1 and Rad51 colocalize and are therefore likely to act together during recombination. (semanticscholar.org)
  • The recombinases DMC1 and RAD51 are functionally and spatially separated during meiosis in Arabidopsis. (semanticscholar.org)
  • It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. (nih.gov)
  • The complex PSMC3IP/MND1 binds DNA, stimulates the recombinase activity of DMC1 as well as DMC1 D-loop formation from double-strand DNA. (nih.gov)
  • This complex stabilizes presynaptic RAD51 and DMC1 filaments formed on single strand DNA to capture double-strand DNA. (nih.gov)
  • Due to its expression pattern and sequence similarity to other RAD51 family members, it is likely that Rad51d is part of a complex of proteins required for DNA repair and meiotic recombination. (nih.gov)
  • Rad51 filament formation and strand invasion is regulated by many mediator proteins such as the Rad51 paralogues and the Shu complex, consisting of a Shu2/SWS1 family member and additional Rad51 paralogues. (nih.gov)
  • Importantly, the srs2 mutant proteins attenuated for Rad51 interaction are much less capable of Rad51 presynaptic filament disruption. (nih.gov)
  • During DNA repair , RAD51 interacts directly or indirectly with a number of breast cancer susceptiblility proteins, such as BRCA1 and BRCA2 . (novusbio.com)
  • This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). (genecards.org)
  • FA proteins interact with several of the proteins involved in HR including H2AX, BRCA1, RAD51 and BLM. (els.net)
  • The proteins encoded by BRCA genes bind with RAD51, a gene product which aids in the repair of damaged DNA. (news-medical.net)
  • Defective proteins in the complex weaken the RAD51 interaction and compromise the DNA repair mechanism. (news-medical.net)
  • We created a new SRS2 allele [ srs2 ( 1-850 )] that lacks the whole C terminus, containing the interaction site for Rad51 and PCNA and interactions with many other proteins. (asm.org)
  • HR is mediated through multiple proteins, including the Rad51/Rad52 recombinases and BRCA2. (asm.org)
  • mediator proteins}, language = {eng}, issn = {0021-9258}, journal = {Journal of Biological Chemistry}, title = {Interaction with Rad51 is indispensable for recombination mediator function of Rad52. (muni.cz)
  • However, it remains largely unknown regarding the NS biochemical characteristics and its functional interaction with RAD51-mediated DNA exchange, due to the hurdle of obtaining the NS recombinant proteins for the biochemical and functional analyses. (ncl.edu.tw)
  • HR is initiated by polymerization of Rad51 on single-stranded DNA at sites of DNA damage, leading to formation of nucleoprotein filaments ( 3 , 33 , 50 , 72 ). (asm.org)
  • In vivo and in vitro studies confirmed that Rad51 filaments formation is not affected by these mutations. (elifesciences.org)
  • Instead, we found that Rad52-Rad51 association makes Rad51 filaments toxic in Srs2-deficient cells after exposure to DNA damaging agents, independently of Rad52 role in Rad51 filament assembly. (elifesciences.org)
  • Importantly, we also demonstrated that Rad52 is essential for protecting Rad51 filaments against dissociation by the Srs2 DNA translocase. (elifesciences.org)
  • It was shown in yeast that the DNA translocase Srs2 is required to avoid the accumulation of toxic Rad51 filaments. (elifesciences.org)
  • However, this control of toxic filaments suggests that functional Rad51 filaments are protected from Srs2 activity. (elifesciences.org)
  • Rad51 is involved in DNA REPAIR of double-strand breaks. (ucdenver.edu)
  • Recent advances using high-resolution microscopy and single molecule techniques have broadened our knowledge of Rad51 filament formation and strand invasion at double-strand break (DSB) sites and at replication forks, which are one of most physiologically relevant forms of HR from yeast to humans. (nih.gov)
  • RAD51 is involved in the search for homology and strand pairing stages of the process. (wikipedia.org)
  • The archaeon Sulfolobus solfataricus RadA recombinase catalyzes DNA pairing and strand exchange, central steps in recombinational repair. (wikipedia.org)
  • Polyamines enhance the DNA strand exchange activity of RAD51 recombinase. (wikipedia.org)
  • To determine the significance of Sgs1 binding to the strand-exchange factor Rad51 , we have identified a single amino acid change at the C-terminal of the helicase core of Sgs1 that disrupts Rad51 binding. (genetics.org)
  • If the Rad51 -mediated homology search is successful, the 3′ end of the invading strand is extended by DNA synthesis. (genetics.org)
  • Strand invasion by the RAD51 nucleoprotein filament produces a four-way DNA junction (or Holliday junction) which is migrated and processed to repair the damage. (novusbio.com)
  • HR depends on the formation of a RAD51 recombinase filament that facilitates strand invasion. (go.jp)
  • However, the biological significance of impaired Rad51 focus formation has not been clarified, because brca2tr cells exhibit only marginal IR sensitivity and thus retain a substantial fraction of the Rad51-dependent capability for repair of IR-induced double strand breaks (DSBs). (asm.org)
  • Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. (genecards.org)
  • Moreover, Rad52 physically interacts with the Rad51 recombinase and serves as a mediator in the Rad51-catalyzed DNA strand exchange reaction. (muni.cz)
  • Filament formation activates the catalytic activities of a recombinase, including ATP hydrolysis and DNA strand exchange. (uvm.edu)
  • We are interested to determine how mutations in the human hRAD51 recombinase, which is essential for the accurate repair of DNA double-strand breaks and for genome stability, are related to cancer. (uvm.edu)
  • Cousineau I, Abaji C, Belmaaza A. BRCA1 regulates RAD51 function in response to DNA damage and suppresses spontaneous sister chromatid replication slippage: implications for sister chromatid cohesion, genome stability, and carcinogenesis. (nih.gov)
  • RAD51 plays critical roles in structural changes and stability of stalled replication sites under replication stress. (eurekalert.org)
  • These suggest that a series of processes starting with RAD51 recruitment and leading to structural changes, breakage, and eventual replication restart are regulated by ATAD5. (eurekalert.org)
  • Augmented HR appears to underpin temozolomide resistance in the recurrent lines, as these cells were cross-resistant to other agents that induced replication-associated DSBs, exhibited faster resolution of damage-induced Rad51 foci, and displayed higher levels of sister chromatid exchanges (SCE). (aacrjournals.org)
  • Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. (nih.gov)
  • Consistent with the genetic data, purified Srs2 strongly inhibits Rad51-mediated recombination reactions by disrupting the Rad51-ssDNA presynaptic filament. (nih.gov)
  • it is generally thought that its role is to suppress HR events at an early stage by dismantling the Rad51-presynaptic filament ( 4 , 5 ). (asm.org)
  • Allosteric communication between adjacent recombinase subunits within the presynaptic filament is essential for recombination. (uvm.edu)
  • Where RAD51 expression was measured in conjunction with BRCA1 expression, an inverse correlation was found. (wikipedia.org)
  • The over expression of RAD51 seen in many cancers may reflect compensatory RAD51 over expression (as in BRCA1 deficiency) and increased HRR to at least partially deal with such excess DNA damages. (wikipedia.org)
  • Circumstantial evidence suggests that the BRCA1/BRCA2/Rad51 complex acts particularly during the S and G2 phases of the cell cycle, suggesting a potential role in sister chromatid recombination. (pewtrusts.org)
  • We are using biochemical and genetic approaches in an effort to determine precisely how BRCA1 and BRCA2 regulate Rad51 functions and to understand the relationship between recombination and breast/ovarian cancer. (pewtrusts.org)
  • Rad51 IRIF are reduced in BRCA1 -null cells ( 5 ). (asm.org)
  • Although recent evidence supports at least Xrcc3 and Rad51C playing a role late in HR, our data suggest that Brca2 and the Rad51 paralogs may also contribute to HR at the same early step, with their loss resulting in the stimulation of an alternative, error-prone repair pathway. (asm.org)
  • RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. (osti.gov)
  • Identification and purification of two distinct complexes containing the five RAD51 paralogs. (semanticscholar.org)
  • DNA damage induced by etoposide was accompanied by the formation of RAD51 foci that were colocalized with γH2AX. (go.jp)
  • To study the relationship between recombination and chromosome pairing, we examined the distribution of RAD51 foci on meiotic chromosomes in maize mutants with defects in chromosome pairing. (plantcell.org)
  • The mutants generally exhibited significant decreases in the number of RAD51 foci at zygotene, corresponding to the degree of their pairing defects. (plantcell.org)
  • CeBRC-2 was able to bind to mammalian Rad51 (mRad51) and form distinct nuclear foci when they interacted. (deepdyve.com)
  • When CeBRC-2 was introduced into BRCA2-deficient cells, it restored Rad51 foci after DNA damage. (deepdyve.com)
  • We found that hundreds of Rad51 foci appeared at leptotene. (msu.ru)
  • From early to late zygotene the number of Rad51 foci gradually decreased. (msu.ru)
  • We did not detect a preferential association of Rad51 foci with unpaired or non-homologously paired regions of lateral elements of synaptonemal complexes in Robertsonian multivalents. (msu.ru)
  • The effects of PTEN on the sensitivity to olaparib and ionizing radiation (IR) exposure were compared between parental HEC-6 (PTEN-null) and HEC-6 PTEN + (stably expressing wild-type PTEN) cells by clonogenic assay, foci formation of RAD51 and γH2AX, and induction of cleaved PARP. (biomedcentral.com)
  • The number of colonies in the clonogenic assay, the foci formation of RAD51 and γH2AX, and the induction of apoptosis were not affected by PTEN introduction in the HEC-6 PTEN + cells. (biomedcentral.com)
  • To investigate the functional relevance of the Srs2-Rad51 complex, we have generated srs2 truncation mutants that retain full ATPase and helicase activities, but differ in their ability to interact with Rad51. (nih.gov)
  • The patterns of RAD51 distribution showed dramatic variation among the meiotic mutants. (plantcell.org)
  • Comparison of the characteristics of brca2tr cells with those of other HR-deficient DT40 clones revealed marked similarities with rad51 paralog mutants ( rad51b , rad51c , rad51d , xrcc2 , or xrcc3 cells). (asm.org)
  • To investigate recombinase allosterism, we generated a series of site-directed mutants in the yeast Rad51 recombinase that alter its allosteric, DNA-binding, and catalytic properties. (uvm.edu)
  • Currently we are using structural and bioinformatic predictions to generate new allosteric mutants of Rad51, which we will characterize both biochemically and structurally. (uvm.edu)
  • Here, we demonstrate that short-term silencing of the Rad51 gene by specific small interfering RNA (siRNA) that inhibits cell proliferation and reduces the viability of most cells. (springer.com)
  • Cells with suppressed expression of Rad51 gene have altered cell cycles and accumulate in the S and G 2 phases. (springer.com)
  • MCF-7 cells, are insensitive to the suppression of Rad51 gene expression. (springer.com)
  • At least four RAD51 gene mutations have been identified in people with congenital mirror movement disorder, a condition in which intentional movements of one side of the body are mirrored by involuntary movements of the other side. (nih.gov)
  • Here we describe a new RAD51-related mammalian gene, named Rad51d, identified by searching the EST database with the yeast RAD55 and human RAD51B/REC2 genes. (nih.gov)
  • RAD51 is a eukaryotic gene . (wikipedia.org)
  • DNA recombinases are widely used in multicellular organisms to manipulate the structure of genomes, and to control gene expression. (wikipedia.org)
  • It has been shown that mutations of RAD51 gene are associated with an increased risk of developing breast cancer (5). (novusbio.com)
  • The phenotypic similarities include a shift from HR-mediated diversification to single-nucleotide substitutions in the immunoglobulin variable gene segment and the partial reversion of this shift by overexpression of Rad51. (asm.org)
  • An important paralog of this gene is RAD51 . (genecards.org)
  • Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. (osti.gov)
  • This is the first study evaluating the role of the RAD51 G172T genetic variants in cancer prognosis and clinical outcome of cervical cancer patients. (biomedsearch.com)
  • Our results indicate an influence of the RAD51 genetic variants in overall survival of cervical cancer. (biomedsearch.com)
  • Recombinases are genetic recombination enzymes. (wikipedia.org)
  • In humans, over- or under-expression of Rad51 occurs in a wide variety of cancers. (wikipedia.org)
  • In cultured mammalian cells, unregulated RAD51 expression enhances resistance to ionizing radiation when irradiated in late S/G2 phase of the cell cycle (3). (novusbio.com)
  • Here, we show that CeBRC-2, which is only one tenth the size of mammalian BRCA2, complemented BRCA2-deficiency in Rad51 regulation. (deepdyve.com)
  • In mammalian cells, RAD51 filament formation is mainly driven by BRCA2 and the role of RAD52 is still enigmatic ( Liu and Heyer, 2011 ). (elifesciences.org)
  • suggests that RAD52 has a role in RAD51 filament regulation also in mammalian cells. (elifesciences.org)
  • We have previously shown that a 28-amino acid peptide derived from the BRC4 motif of BRCA2 tumor suppressor inhibits selectively human RAD51 recombinase (HsRad51). (chalmers.se)
  • A Rec A recombinase found in eukaryotes. (ucdenver.edu)
  • Such recombinases have been described in archaea, bacteria, eukaryotes and viruses. (wikipedia.org)
  • Studies to define the biochemical and biological features of the BRCA2:RAD51 interaction are described. (bl.uk)
  • Further, RAD51 was unable to interact effectively with a BRC repeat motif in which the proposed interaction interface had been disrupted by mutagenesis. (bl.uk)
  • Kinetic data for the interaction of an individual BRC repeat motif with RAD51 were obtained. (bl.uk)
  • An SPR competition assay was developed, revealing that the binding affinity of each BRC repeat motif for RAD51 differs significantly, and that their organisation within the scaffold of BRCA2 contributes to efficient interaction. (bl.uk)
  • An internal deletion in Srs2 likewise diminishes Rad51 interaction and anti-recombinase activity. (nih.gov)
  • These results highlight the importance of Rad51 interaction in the anti-recombinase function of Srs2, and provide evidence that this Srs2 function can be uncoupled from its helicase activity. (nih.gov)
  • A ) Fragments of Srs2 were tested for two-hybrid interaction with Rad51 by plating on medium lacking leucine, tryptophan and adenine. (nih.gov)
  • Mapping of Rad51 interaction by in vitro pull-down assay. (nih.gov)
  • Point mutations in the BCR repeat sequence can compromise the interaction with RAD51. (news-medical.net)
  • The relative importance of Srs2's helicase activity, Rad51 removal function, and PCNA interaction in genome stability remains unclear. (asm.org)
  • Interaction with Rad51 is indispensable for recombination mediator function of Rad52. (muni.cz)
  • Here, we examine the functional significance of the Rad51/Rad52 interaction. (muni.cz)
  • Through a series of deletions, we have identified residues 409-420 of Rad52 as being indispensable and likely sufficient for its interaction with Rad51. (muni.cz)
  • We have constructed a four-amino acid deletion mutation within this region of Rad52 to ablate its interaction with Rad51. (muni.cz)
  • The functional significances of DNA binding and RAD51 interaction by NS on RAD51-mediated DSB repair will be examined in the near future. (ncl.edu.tw)
  • The current model assumes that Rad51 filament formation requires the interaction between Rad52 and Rad51. (elifesciences.org)
  • In yeast, Rad52 is the most important mediator of Rad51 filament formation and it was proposed that this function involves its interaction with both RPA and Rad51. (elifesciences.org)
  • These results provide evidence for a key role of RAD51 structures in the homology search. (plantcell.org)
  • To accomplish heteroduplex formation, the RAD51 nucleoprotein filament has the ability to recognize DNA sequence homology. (plantcell.org)
  • Selective chromatid segregation mechanism invoked for the human congenital mirror hand movement disorder development by RAD51 mutations: a hypothesis. (nih.gov)
  • Amplification of the Mutation-Carrying BRCA2 Allele Promotes RAD51 Loading and PARP Inhibitor Resistance in the Absence of Reversion Mutations. (ucdenver.edu)
  • Mutations in the Rad51-binding domain of Rad52 suppress MMS and γ-ray sensitivity of Srs2-deficient cells. (elifesciences.org)
  • One of the critical genes for DNA repair and meiotic recombination in yeast is RAD51, and homologs of RAD51 have been identified in several species, including mouse and human. (nih.gov)
  • The structural basis for Rad51 filament formation and its functional mechanism still remain poorly understood. (wikipedia.org)
  • Interestingly, depletion of NS will significantly attenuate the recruitment of RAD51 to DSBs. (ncl.edu.tw)
  • Our results indicate that RAD51 plays a critical role in maintaining chromosome integrity and mitochondrial activity during porcine oocyte maturation. (go.jp)
  • Srs2 interacts with Rad51 in the yeast two-hybrid assay and also in vitro. (nih.gov)
  • Two-hybrid interactions of Rad51 with Srs2. (nih.gov)
  • In panel II, Srs2 and srs2 Δ875-902 were combined with purified Rad51 and mixed with nickel NTA agarose beads. (nih.gov)
  • We find that the interactions of Srs2 with Rad51 and PCNA are dispensable for the main role of Srs2 in the repair of DNA damage in vegetative cells and for proper completion of meiosis. (asm.org)
  • The relative roles of the helicase and Rad51 removal activities of Srs2 in genome stability remain unclear. (asm.org)
  • [3] The recombinases catalyze invasion of the opposite chromatid by the single-stranded DNA from one end of the break. (bionity.com)
  • A well-studied example is the UvsX recombinase encoded by bacteriophage T4. (wikipedia.org)
  • Cloning and sequencing full length of canine Brca2 and Rad51 cDNA // Journal of Veterinary Medical Science. (msu.ru)
  • Importantly, a novel RAD51 paralogue was discovered in Caenorhabditis elegans, and its in vitro characterization has demonstrated a new function for the worm RAD51 paralogues during HR. Conservation of the human RAD51 paralogues function during HR and repair of replicative damage demonstrate how the RAD51 mediators play a critical role in human health and genomic integrity. (nih.gov)
  • Together, these new findings provide a framework for understanding RAD51 and its mediators in DNA repair during multiple cellular contexts. (nih.gov)
  • Insights as to how BRCA2 regulates Rad51-mediated DNA repair arose from in vitro biochemical studies on fragments of BRCA2. (deepdyve.com)
  • CDH2 , MMPs , VIM , or ZEB1 and 2 ), DNA repair ( RAD51 ), resistance to cancer therapy (e.g. (aaccjnls.org)
  • Taken all results together, it is clear that nucleostemin participates in the RAD51-mediated recombination repair. (ncl.edu.tw)
  • In summary, our NS purification system and the biochemical properties reported herein should expedite further mechanistic study regarding the mechanistic action of NS in RAD51-mediated DSB repair. (ncl.edu.tw)
  • Inhibition of RAD51 increased DNA damage and induced metaphase I arrest along with spindle defects, chromosomal misalignment, and abnormal spindle assembly checkpoint (SAC) activity. (go.jp)
  • Inhibition of RAD51 also increased ROS levels and led to an abnormal mitochondrial distribution. (go.jp)
  • Human BRCA2 features eight BRC repeat motifs encoded within exon 11 through which it can bind RAD51. (bl.uk)
  • Specific for human Rad51. (abcam.com)
  • Our work establishes remodeling of uncoupled forks as a pivotal RAD51-regulated response to genotoxic stress in human cells and as a promising target to potentiate cancer chemotherapy. (rupress.org)
  • We used antibodies against human Rad51 to examine the temporal and spatial distribution of Rad51 in the spermatocytes of the common shrew Sorex araneus (Eulipotyphla, Soricidae). (msu.ru)
  • Distribution of the Rad51 recombinase in human and mouse spermatocytes // EMBO Journal. (msu.ru)
  • The EVL knockdown cells impaired RAD51 assembly onto damaged DNA after ionizing radiation or mitomycin C treatment. (biomedsearch.com)
  • The BRC repeat motifs are believed to mimic and disrupt RAD51 oligomerisation at its self-association interface. (bl.uk)
  • Thus, I propose that both the differential binding affinities of the individual BRC repeat motifs for RAD51, and their observed cooperativity, contribute to the control of RAD51 by BRCA2. (bl.uk)
  • Using siRNA depletion of RAD51 has demonstrated to reduce drug resistance and improve Gemcitabine-induced cytotoxicity. (novusbio.com)
  • We also show that the sensitivity of the rad52delta409-412 mutant to ionizing radiation can be complemented by overexpression of Rad51. (muni.cz)