A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
A large family of MONOMERIC GTP-BINDING PROTEINS that play a key role in cellular secretory and endocytic pathways. EC 3.6.1.-.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)
Cytoplasmic vesicles formed when COATED VESICLES shed their CLATHRIN coat. Endosomes internalize macromolecules bound by receptors on the cell surface.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Phosphatidylinositols in which one or more alcohol group of the inositol has been substituted with a phosphate group.
A genetically related subfamily of RAB GTP-BINDING PROTEINS involved in transport from the cell membrane to early endosomes. This enzyme was formerly listed as EC 3.6.1.47.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The middle of the three primitive cerebral vesicles of the embryonic brain. Without further subdivision, midbrain develops into a short, constricted portion connecting the PONS and the DIENCEPHALON. Midbrain contains two major parts, the dorsal TECTUM MESENCEPHALI and the ventral TEGMENTUM MESENCEPHALI, housing components of auditory, visual, and other sensorimoter systems.
Two ganglionated neural plexuses in the gut wall which form one of the three major divisions of the autonomic nervous system. The enteric nervous system innervates the gastrointestinal tract, the pancreas, and the gallbladder. It contains sensory neurons, interneurons, and motor neurons. Thus the circuitry can autonomously sense the tension and the chemical environment in the gut and regulate blood vessel tone, motility, secretions, and fluid transport. The system is itself governed by the central nervous system and receives both parasympathetic and sympathetic innervation. (From Kandel, Schwartz, and Jessel, Principles of Neural Science, 3d ed, p766)
Neurons whose primary neurotransmitter is DOPAMINE.
The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.
One of the catecholamine NEUROTRANSMITTERS in the brain. It is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
An enzyme that catalyzes the conversion of L-tyrosine, tetrahydrobiopterin, and oxygen to 3,4-dihydroxy-L-phenylalanine, dihydrobiopterin, and water. EC 1.14.16.2.
A liverwort plant genus of the family Marchantiaceae, order Marchantiales, subclass MARCHANTIAE. Members contain brassinosteroids and DITERPENES.
A plant division. They are simple plants that lack vascular tissue and possess rudimentary rootlike organs (rhizoids). Like MOSSES, liverworts have alternation of generations between haploid gamete-bearing forms (gametophytes) and diploid spore-bearing forms (sporophytes).
The opening and closing of ion channels due to a stimulus. The stimulus can be a change in membrane potential (voltage-gated), drugs or chemical transmitters (ligand-gated), or a mechanical deformation. Gating is thought to involve conformational changes of the ion channel which alters selective permeability.
Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
Protein factors that inhibit the dissociation of GDP from GTP-BINDING PROTEINS.
A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.
A guanine nucleotide containing two phosphate groups esterified to the sugar moiety.
A subcategory of guanine nucleotide dissociation inhibitors that are specific for RHO GTP-BINDING PROTEINS.
Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
A family of immunophilin proteins that bind to the immunosuppressive drugs TACROLIMUS (also known as FK506) and SIROLIMUS. EC 5.2.1.-
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.

Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. (1/1961)

The vacuolar protein sorting (VPS) pathway of Saccharomyces cerevisiae mediates transport of vacuolar protein precursors from the late Golgi to the lysosome-like vacuole. Sorting of some vacuolar proteins occurs via a prevacuolar endosomal compartment and mutations in a subset of VPS genes (the class D VPS genes) interfere with the Golgi-to-endosome transport step. Several of the encoded proteins, including Pep12p/Vps6p (an endosomal target (t) SNARE) and Vps45p (a Sec1p homologue), bind each other directly [1]. Another of these proteins, Vac1p/Pep7p/Vps19p, associates with Pep12p and binds phosphatidylinositol 3-phosphate (PI(3)P), the product of the Vps34 phosphatidylinositol 3-kinase (PI 3-kinase) [1] [2]. Here, we demonstrate that Vac1p genetically and physically interacts with the activated, GTP-bound form of Vps21p, a Rab GTPase that functions in Golgi-to-endosome transport, and with Vps45p. These results implicate Vac1p as an effector of Vps21p and as a novel Sec1p-family-binding protein. We suggest that Vac1p functions as a multivalent adaptor protein that ensures the high fidelity of vesicle docking and fusion by integrating both phosphoinositide (Vps34p) and GTPase (Vps21p) signals, which are essential for Pep12p- and Vps45p-dependent targeting of Golgi-derived vesicles to the prevacuolar endosome.  (+info)

The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. (2/1961)

Polarized secretion requires proper targeting of secretory vesicles to specific sites on the plasma membrane. Here we report that the exocyst complex plays a key role in vesicle targeting. Sec15p, an exocyst component, can associate with secretory vesicles and interact specifically with the rab GTPase, Sec4p, in its GTP-bound form. A chain of protein-protein interactions leads from Sec4p and Sec15p on the vesicle, through various subunits of the exocyst, to Sec3p, which marks the sites of exocytosis on the plasma membrane. Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion.  (+info)

Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. (3/1961)

The small G protein Rab3A plays an important role in the regulation of neurotransmitter release. The crystal structure of activated Rab3A/GTP/Mg2+ bound to the effector domain of rabphilin-3A was solved to 2.6 A resolution. Rabphilin-3A contacts Rab3A in two distinct areas. The first interface involves the Rab3A switch I and switch II regions, which are sensitive to the nucleotide-binding state of Rab3A. The second interface consists of a deep pocket in Rab3A that interacts with a SGAWFF structural element of rabphilin-3A. Sequence and structure analysis, and biochemical data suggest that this pocket, or Rab complementarity-determining region (RabCDR), establishes a specific interaction between each Rab protein and its effectors. RabCDRs could be major determinants of effector specificity during vesicle trafficking and fusion.  (+info)

Identification of a putative effector protein for rab11 that participates in transferrin recycling. (4/1961)

We have identified and cloned the cDNA for a 912-aa protein, rab11BP, that interacts with the GTP-containing active form of rab11, a GTP-binding protein that plays a critical role in receptor recycling. Although rab11BP is primarily cytosolic, a significant fraction colocalizes with rab11 in endosomal membranes of both the sorting and recycling subcompartments. In vitro binding of rab11 to native rab11BP requires partial denaturation of the latter to expose an internal binding site located between residues 334 and 504 that is apparently masked by the C-terminal portion of the protein, which includes six repeats known as WD40 domains. Within the cell, rab11BP must undergo a conformational change in which the rab11-binding site becomes exposed, because when coexpressed with rab11 in transfected cells the two proteins formed abundant complexes in association with membranes. Furthermore, although overexpression of rab11BP did not affect transferrin recycling, overexpression of a truncated form of the protein, rab11BP(1-504), that includes the rab11-binding site but lacks the WD40 domains inhibited recycling as strongly as does a dominant negative rab11 mutant protein that does not bind GTP. Strikingly, the inhibition caused by the truncated rab11BP was prevented completely when the cells also expressed a C-terminally deleted, nonprenylatable form of rab11 that, by itself, has no effect on recycling. We propose that rab11BP is an effector for rab11, whose association with this GTP-binding protein is dependent on the action of another membrane-associated factor that promotes the unmasking of the rab11-binding site in rab11BP.  (+info)

An arrested late endosome-lysosome intermediate aggregate observed in a Chinese hamster ovary cell mutant isolated by novel three-step screening. (5/1961)

Chinese hamster ovary cell mutants defective in the post-uptake degradation of low-density lipoprotein (LDL) in lysosomes were selected from mutagenized cells by novel three-step screening. First, in the presence of LDL, clones sensitive to an inhibitor of the rate-limiting enzyme of the cholesterol biosynthetic pathway, 3-hydroxy-3-methylglutaryl-CoA reductase, were isolated. Second, from the selected clones, those lacking in the degradation of a constituent of a fluorescent LDL were qualitatively screened by microscopy. Third, the clones were further screened by previously established quantitative analytical flow cytometry that detects the early-phase disintegration of LDL by lysosomal acid hydrolases. One of the isolated mutant clones, LEX1 (Lysosome-Endosome X 1), was a recessive mutant, and exhibited a specific disorder in the late endocytic pathway. LEX1 cells showed an unusual perinuclear aggregate of vesicles, heterogeneously positive for lysosomal glycoprotein-B/cathepsin D and rab7, yet negative for the cation-independent mannose 6-phosphate receptor. The aggregate was formed around the microtubule organizing center, and was disrupted by nocodazole treatment. Internalized octadecyl rhodamine B-labeled LDL (R18-LDL) was accumulated in the perinuclear rab7-positive vesicles. In a Percoll density gradient, neither internalized R18-LDL nor internalized horseradish peroxidase was efficiently chased into heavy lysosomal fractions positive for beta-hexosaminidase. LEX1 cells showed differences in the activity and subcellular distribution of lysosomal enzymes. These characteristics of LEX1 cells are consistent with the ideas that the perinuclear vesicle aggregate is an arrested intermediate of direct fusion or divergence between lysosomes and rab7-positive, cation-independent mannose 6-phosphate receptor-negative late endosomes, and that equilibrium between the lysosomes and the late endosomes is shifted towards the late endosomes in LEX1 cells. Such fusion or divergence between the late endosomes and the lysosomes would determine an appropriate equilibrium between them, and might thereby play an important role for proper lysosomal digestive functions. LEX1 mutant cells would be helpful for the dissection of the as yet unrevealed details of the late endocytic membrane dynamics and for the identification of factors involved in the process arrested by the mutation.  (+info)

Primary structure and biochemical characterization of yeast GTPase-activating proteins with substrate preference for the transport GTPase Ypt7p. (6/1961)

Small GTPases of the Ypt/Rab family are regulators of vesicular protein trafficking in exo-and endocytosis. GTPase-activating proteins (GAP) play an important role as down regulators of GTPases. We here report the molecular cloning of a novel GAP-encoding gene (GYP7, for GAP for Ypt7) by high expression from a Saccharomyces cerevisiae genomic library. The GYP7 gene encodes a hydrophilic protein with a molecular mass of 87 kDa. Comparison of its primary sequence with that of the three other known GAPs for transport GTPases, the yeast Gyp6 and Gyp1 proteins and the Rab3A-GAP from rat brain, shows similarity between the yeast GAPs only. Like GYP6 and GYP1, GYP7 is not essential for yeast cell viability. Gyp7p was able to most effectively accelerate the intrinsic GTPase activity of Ypt7p. It was also active, but to a lesser extent, on Ypt31p, Ypt32p and Ypt1p. Ypt6p, Sec4p and the human H-Ras protein did not serve as substrates. We also report the identification and cloning of a gene from the dimorphic yeast Yarrowia lipolytica that encodes a protein whose primary structure and biochemical activity are significantly related to those of Gyp7p from baker's yeast.  (+info)

The receptor recycling pathway contains two distinct populations of early endosomes with different sorting functions. (7/1961)

Receptor recycling involves two endosome populations, peripheral early endosomes and perinuclear recycling endosomes. In polarized epithelial cells, either or both populations must be able to sort apical from basolateral proteins, returning each to its appropriate plasma membrane domain. However, neither the roles of early versus recycling endosomes in polarity nor their relationship to each other has been quantitatively evaluated. Using a combined morphological, biochemical, and kinetic approach, we found these two endosome populations to represent physically and functionally distinct compartments. Early and recycling endosomes were resolved on Optiprep gradients and shown to be differentially associated with rab4, rab11, and transferrin receptor; rab4 was enriched on early endosomes and at least partially depleted from recycling endosomes, with the opposite being true for rab11 and transferrin receptor. The two populations were also pharmacologically distinct, with AlF4 selectively blocking export of transferrin receptor from recycling endosomes to the basolateral plasma membrane. We applied these observations to a detailed kinetic analysis of transferrin and dimeric IgA recycling and transcytosis. The data from these experiments permitted the construction of a testable, mathematical model which enabled a dissection of the roles of early and recycling endosomes in polarized receptor transport. Contrary to expectations, the majority (>65%) of recycling to the basolateral surface is likely to occur from early endosomes, but with relatively little sorting of apical from basolateral proteins. Instead, more complete segregation of basolateral receptors from receptors intended for transcytosis occurred upon delivery to recycling endosomes.  (+info)

Characterization of GAPCenA, a GTPase activating protein for Rab6, part of which associates with the centrosome. (8/1961)

The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification and characterization of a novel human Rab6-interacting protein named human GAPCenA (for 'GAP and centrosome-associated'). Primary sequence analysis indicates that GAPCenA displays similarities, within a central 200 amino acids domain, to both the yeast Rab GTPase activating proteins (GAPs) and to the spindle checkpoint proteins Saccharomyces cerevisiae Bub2p and Schizosaccharomyces pombe Cdc16p. We demonstrate that GAPCenA is indeed a GAP, specifically active in vitro on Rab6 and, to a lesser extent, on Rab4 and Rab2 proteins. Immunofluorescence and cell fractionation experiments showed that GAPCenA is mainly cytosolic but that a minor pool is associated with the centrosome. Moreover, GAPCenA was found to form complexes with cytosolic gamma-tubulin and to play a role in microtubule nucleation. Therefore, GAPCenA may be involved in the coordination of microtubule and Golgi dynamics during the cell cycle.  (+info)

The C2A domain of synaptotagmin-like protein 3 (Slp3) is an atypical calcium-dependent phospholipid-binding machine: comparison with the C2A domain of synaptota
TY - JOUR. T1 - Rab family small GTPases-mediated regulation of intracellular logistics in neural development. AU - Shikanai, Mima. AU - Yuzaki, Michisuke. AU - Kawauchi, Takeshi. PY - 2018/8/1. Y1 - 2018/8/1. N2 - Rab family small GTPases play essential roles in various cellular events via the regulation of intracellular logistics comprising a large number of membrane traffic pathways. Emerging evidence reveals the physiological roles of Rab proteins in several tissues, including developing brains. Many Rab proteins, such as Rab5, Rab6, Rab7, Rab8, Rab10, Rab11, Rab17 and Rab18, are shown to regulate neurite outgrowth in PC12 cells and/or axon and dendrite formation in primary cultured neurons. Recent studies have also revealed in vivo roles of several Rab family small GTPases in brain development and its related neurological disorders. In this review, we introduce the physiological function of Rab family proteins in neural development with particular focus on neurite outgrowth and neuronal ...
Rab27 is a member of the Rab subfamily of Ras GTPase proteins. Rab27 protein is membrane-bound and is post-translationally modified by the addition of geranylgeranyl groups on two C-terminal cysteines. Two isoforms of Rab27 are known, encoded by the RAB27A and RAB27B genes. Rab27A and Rab27B were found to control different steps of the exosome secretion pathway. Rab27 proteins are involved in melanosome transport in melanocytes and in killing activity in cytotoxic T lymphocytes. In addition, they have been suggested to play roles in protein transport and small GTPase-mediated signal transduction. Mutations in the RAB27A gene are associated with Griscelli syndrome type 2, a disease characterized by hypopigmentation and immunodeficiency. Rab27A is also known as RAB27A, member RAS oncogene family; GTP-binding protein Ram, RAB27, GS2, RAM, and HsT18676. Rab27B is also known as RAB27B, member RAS oncogene family; and C25KG2.. ...
Rab27 is a member of the Rab subfamily of Ras GTPase proteins. Rab27 protein is membrane-bound and is post-translationally modified by the addition of geranylgeranyl groups on two C-terminal cysteines. Two isoforms of Rab27 are known, encoded by the RAB27A and RAB27B genes. Rab27A and Rab27B were found to control different steps of the exosome secretion pathway. Rab27 proteins are involved in melanosome transport in melanocytes and in killing activity in cytotoxic T lymphocytes. In addition, they have been suggested to play roles in protein transport and small GTPase-mediated signal transduction. Mutations in the RAB27A gene are associated with Griscelli syndrome type 2, a disease characterized by hypopigmentation and immunodeficiency. Rab27A is also known as RAB27A, member RAS oncogene family; GTP-binding protein Ram, RAB27, GS2, RAM, and HsT18676. Rab27B is also known as RAB27B, member RAS oncogene family; and C25KG2.. ...
Rab guanosine triphosphatases (GTPases) regulate vesicle trafficking in eukaryotic cells by reversibly associating with lipid membranes. Inactive Rab GTPases are maintained in the cytosol by binding to GDP-dissociation inhibitor (GDI). It is believed that specialized proteins are required to displace GDI from Rab GTPases before Rab activation by guanosine diphosphate-guanosine 5′-triphosphate (GDP-GTP) exchange factors (GEFs). Here, we found that SidM from Legionella pneumophila could act as both GEF and GDI-displacement factor (GDF) for Rab1. Rab1 released from GDI was inserted into liposomal membranes and was used as a substrate for SidM-mediated nucleotide exchange. During host cell infection, recruitment of Rab1 to Legionella-containing vacuoles depended on the GDF activity of SidM. Thus, GDF and GEF activity can be promoted by a single protein, and GDF activity can coordinate Rab1 recruitment from the GDI-bound pool.. ...
Rab GTPases and their effectors facilitate vesicular transport by tethering donor vesicles to their respective target membranes. Rab9 mediates late endosome to trans-Golgi transport and has recently been found to be a key cellular component for human immunodeficiency virus-1, Ebola, Marburg, and measles virus replication, suggesting that it may be a novel target in the development of broad spectrum antiviral drugs. As part of our structure-based drug design program, we have determined the crystal structure of a C-terminally truncated human Rab9 (residues 1-177) to 1.25-A resolution. The overall structure shows a characteristic nucleotide binding fold consisting of a six-stranded beta-sheet surrounded by five alpha-helices with a tightly bound GDP molecule in the active site. Structure-based sequence alignment of Rab9 with other Rab proteins reveals that its active site consists of residues highly conserved in the Rab GTPase family, implying a common catalytic mechanism. However, Rab9 contains ...
Rab effectors, defined as proteins that interact specifically with the GTP-bound from of a Rab GTPase, come in many flavours and include molecular tethers, fusion regulators, motors, sorting adaptors, kinases, phosphatases, components of membrane contact sites and Rab regulators (Gillingham et al., 2014). The recruitment of such effectors in a spatiotemporally controlled manner contributes strongly to the fidelity and specificity of intracellular membrane traffic. There are also a few examples of proteins that are regulated by GDP-bound Rabs or that interact with Rabs in a nucleotide-independent fashion, including the interactions between Rab21 and β1-integrin, Rab11 and protrudin, Rab7 and VPS34, and Rab27a and Coronin3 (Kimura et al., 2008; Pellinen et al., 2006; Shirane and Nakayama, 2006; Stein et al., 2003); however, the term effector should be reserved for those proteins that interact exclusively with the GTP-bound form of a Rab GTPase. In some cases, different Rab GTPases bind to ...
Rab proteins are small GTPases that play important roles in transport of vesicle cargo and recruitment, association of motor and other proteins with vesicles, and docking and fusion of vesicles at defined locations. In vertebrates, ,75 Rab genes have been identified, some of which have been intensively studied for their roles in endosome and synaptic vesicle trafficking. Recent studies of the functions of certain Rab proteins have revealed specific roles in mediating developmental signal transduction. We have begun a systematic genetic study of the 33 Rab genes in Drosophila. Most of the fly proteins are clearly related to specific vertebrate proteins. We report here the creation of a set of transgenic fly lines that allow spatially and temporally regulated expression of Drosophila Rab proteins. We generated fluorescent protein-tagged wild-type, dominant-negative, and constitutively active forms of 31 Drosophila Rab proteins. We describe Drosophila Rab expression patterns during embryogenesis, ...
The endoplasmic reticulum (ER), the Golgi apparatus, the vacuole and the plasma membrane constitute the major components of the plant secretory system. The communication between these organelles is mediated by small GTPases of the Rab subfamily (Stenmark and Olkkonen, 2001). Members of this family are localized to distinct membrane compartments and exert functions in different trafficking steps in the secretory and endocytic pathways. Specifically, Rab proteins are involved in the recruitment of effector molecules on cellular membranes, which in turn regulate membrane docking and fusion (Stenmark and Olkkonen, 2001).. Rab GTPases are soluble proteins of ∼23 kDa and contain conserved amino acid sequences, which are necessary for nucleotide binding and GTP hydrolysis (Olkkonen and Stenmark, 1997). They act as molecular switches that regulate intracellular transport cycling between a target membrane and the cytosol, by alternating between GTP and GDP conformations (Bourne et al., 1991; Nuoffer ...
The Federal Information Processing Standard (FIPS) Publication 140-3 was a proposed update to the U.S. government computer security standard used to accredit cryptographic modules. The title of the standard is Security Requirements for Cryptographic Modules and FIPS 140-2 remains the currently approved version. Efforts to update FIPS 140-2 date back to the early 2000s. The FIPS 140-3 (2013 Draft) was scheduled for signature by the Secretary of Commerce in August 2013, however that never happened and the draft was subsequently abandoned. In 2014, NIST released a substantially different draft of FIPS 140-3, this version effectively directing the use of an International Organization for Standardization/International Electrotechnical Commission (ISO/IEC) standard, 19790:2012, as the replacement for FIPS 140-2. The 2014 draft of FIPS 140-3 was also abandoned. On August 12, 2015, NIST formally released a statement on the Federal Register asking for comments on the potential use of portions of ISO/IEC ...
Rab are small GTPases implicated in vesicle trafficking. Like the other small GTPases, Rab proteins act as molecular switches, with an active GTP-bound form that interacts with its target or effector protein and an inactive GDP-bound form. A subgroup of Rab effectors contain in their N-terminal part a conserved region of around 70 amino acid residues, the Rab-binding domain (RabBD) . In some Rab effector domains an atypical FYVE-type zinc finger is inserted in the central part [1]. The crystal structure of the Rab effector domain of Rabphilin-3A in complex with Rab3A has been solved [2] (see ,PDB:1ZBD,). The structure consists of two long helices separated by an atypical FYVE-type zinc finger which adopts a conformation similar to classical ones (see ,PDOC50178,). The central zinc finger does not directly interact with Rab3A. The amino acids important for this interaction are located around a short C-terminal motif (SGAWFF) and an acidic cluster in the N-terminal area. Proteins known to contain ...
Rab GTPases are implicated in endosome-to-plasma membrane recycling, but how such membrane traffic regulators control vascular endothelial growth factor receptor 2 (VEGFR2/KDR) dynamics and function are not well understood. Here, we evaluated two different recycling Rab GTPases, Rab4a and Rab11a, in regulating endothelial VEGFR2 trafficking and signalling with implications for endothelial cell migration, proliferation and angiogenesis. In primary endothelial cells, VEGFR2 displays co-localisation with Rab4a, but not Rab11a GTPase, on early endosomes. Expression of a guanosine diphosphate (GDP)-bound Rab4a S22N mutant caused increased VEGFR2 accumulation in endosomes. TfR and VEGFR2 exhibited differences in endosome-to-plasma membrane recycling in the presence of chloroquine. Depletion of Rab4a, but not Rab11a, levels stimulated VEGF-A-dependent intracellular signalling. However, depletion of either Rab4a or Rab11a levels inhibited VEGF-A-stimulated endothelial cell migration. Interestingly, depletion of
Rab GTPase family 3 contains Rab3A, Rab3B, Rab3C and Rab3D. The Rab3 subfamily contains Rab3A, Rab3B, Rab3C, and Rab3D. All four isoforms were found in mouse brain and endocrine tissues, with varying levels of expression. Rab3A, Rab3B, and Rab3C localized to synaptic and secretory vesicles; Rab3D was expressed at high levels only in adipose tissue, exocrine glands, and the endocrine pituitary, where it is localized to cytoplasmic secretory granules. Rab3 appears to control Ca2+-regulated exocytosis. The appropriate GDP/GTP exchange cycle of Rab3A is required for Ca2+-regulated exocytosis to occur, and interaction of the GTP-bound form of Rab3A with effector molecule(s) is widely believed to be essential for this process. Functionally, most studies point toward a role for Rab3 in the secretion of hormones and neurotransmitters. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with ...
Rab GTPases are key master regulators of membrane maturation and trafficking throughout the endomembrane system of eukaryotic cells. The function of Rab GTPases...
antibody-antibodies.com is the marketplace for research antibodies. Find the right antibody for your research needs. Deregulation of Rab and Rab Effector Genes in Bladder Cancer
Rab GTPases define the vesicle trafficking pathways underpinning cell polarization and migration. Here, we find that Rab4, Rab11, and Rab14 and the candidate Rab GDP-GTP exchange factors (GEFs) FAM116A and AVL9 are required for cell migration. Rab14 and its GEF FAM116A localize to and act on an intermediate compartment of the transferrin-recycling pathway prior to Rab11 and after Rab5 and Rab4. This Rab14 intermediate recycling compartment has specific functions in migrating cells discrete from early and recycling endosomes. Rab14-depleted cells show increased N-cadherin levels at junctional complexes and cannot resolve cell-cell junctions. This is due to decreased shedding of cell-surface N-cadherin by the ADAM family protease ADAM10/Kuzbanian. In FAM116A- and Rab14-depleted cells, ADAM10 accumulates in a transferrin-positive endocytic compartment, and the cell-surface level of ADAM10 is correspondingly reduced. FAM116 and Rab14 therefore define an endocytic recycling pathway needed for ADAM protease
The monomeric small GTPase Rab27a is specifically localized on both secretory granules and lysosome-related organelles. Although natural mutations of the Rab27a gene in human Griscelli syndrome and in ashen mice cause partial albinism and immunodeficiency reflecting the dysfunction of lysosome-related organelles, phenotypes resulting from the defective exocytosis of secretory granules have not been reported. To explore the roles of Rab27a in secretory granules, we analyzed insulin secretion profiles in ashen mice. Ashen mice showed glucose intolerance after a glucose load without signs of insulin resistance in peripheral tissues or insulin deficiency in the pancreas. Insulin secretion from isolated islets was decreased specifically in response to high glucose concentrations but not other nonphysiological secretagogues such as high K+ concentrations, forskolin, or phorbol ester. Neither the intracellular Ca2+ concentration nor the dynamics of fusion pore opening after glucose stimulation were ...
The monomeric small GTPase Rab27a is specifically localized on both secretory granules and lysosome-related organelles. Although natural mutations of the Rab27a gene in human Griscelli syndrome and in ashen mice cause partial albinism and immunodeficiency reflecting the dysfunction of lysosome-related organelles, phenotypes resulting from the defective exocytosis of secretory granules have not been reported. To explore the roles of Rab27a in secretory granules, we analyzed insulin secretion profiles in ashen mice. Ashen mice showed glucose intolerance after a glucose load without signs of insulin resistance in peripheral tissues or insulin deficiency in the pancreas. Insulin secretion from isolated islets was decreased specifically in response to high glucose concentrations but not other nonphysiological secretagogues such as high K+ concentrations, forskolin, or phorbol ester. Neither the intracellular Ca2+ concentration nor the dynamics of fusion pore opening after glucose stimulation were ...
Herein, we provide evidence for a Rab-dependent mechanism for regulating VEGFR2 trafficking and signaling from endosomes in primary endothelial cells. Manipulation of GTPase activity through overexpression of GFP-tagged wild-type or mutant Rab7a proteins perturbed endothelial VEGFR2 trafficking, degradation, and signaling. Intriguingly, Rab7a depletion decreased VEGFR2 tyrosine autophosphorylation on residue Y1175, a characteristic signature in VEGFR2-mediated activation and signaling.32 This correlated with ≈50% decrease in VEGF-A-stimulated endothelial cell migration after Rab7a depletion, suggesting a requirement for Rab7a in VEGFR2-mediated signaling. However, in Rab7a-depleted cells, there was increased p42/44 MAPK levels 30 minutes after VEGF-A addition, suggesting increased signaling through this arm of the pathway. A likely explanation is that VEGFR2-regulated signaling to p42/p44 MAPK occurs at the early endosome and is prolonged in Rab7a-depleted cells as VEGFR2 cannot progress ...
This thesis work describes the role of Rab proteins in amyloid processing and clearance in different cell pathways. It also describes an experimental approach used to analyze the expression effects of Rab10 in amyloid beta production. Since the main theory behind neurodegeneration in Alzheimers disease claims that high levels of amyloid beta 42 (Aβ42) molecules trigger widespread neuronal death, control of Aβ42 has been a main target in Alzheimers disease research. In addition, several studies show increased levels of particular Rab proteins in Alzheimers pathogenesis. However, no review consolidates current findings in neurodegeneration of Alzheimers with Rab protein dysfunction. The first chapter of this thesis aims to address this need by providing a current review of Rab proteins associated with APP and neurodegeneration. The second chapter constitutes an experimental approach used to characterize the effects of Rab10 and Sar1A GTPases in APP and amyloid processing. We found that Rab10
The Goldenring laboratory studies a number of subjects that apply broadly to epithelial biology. We pursue three distinct programmatic areas. First, we are investigating the protein machinery required for the regulation of the recycling to the plasma membrane of receptors, ion transporters and ion channels. These studies focus on the ability of the Rab11 family of small GTPases (Rab11a, Rab11b and Rab25) to initiate and coordinate the assembly of multiprotein complexes regulating vesicle trafficking. Over the past several years, we have identified myosin Vb as the molecular motor involved in movement towards the plasma membrane. We have also identified a family of at least 8 other Rab11 family interacting proteins (Rab11-FIPs) that also participate in the regulation of plasma membrane recycling. Present investigations center on understanding the assembly of endogenous complexes, the regulation of recycling by phosphorylation of Rab11-FIP proteins by the kinase Par1b/MARK2, and the influence of Rab11
The Goldenring laboratory studies a number of subjects that apply broadly to epithelial biology. We pursue three distinct programmatic areas. First, we are investigating the protein machinery required for the regulation of the recycling to the plasma membrane of receptors, ion transporters and ion channels. These studies focus on the ability of the Rab11 family of small GTPases (Rab11a, Rab11b and Rab25) to initiate and coordinate the assembly of multiprotein complexes regulating vesicle trafficking. Over the past several years, we have identified myosin Vb as the molecular motor involved in movement towards the plasma membrane. We have also identified a family of at least 8 other Rab11 family interacting proteins (Rab11-FIPs) that also participate in the regulation of plasma membrane recycling. Present investigations center on understanding the assembly of endogenous complexes, the regulation of recycling by phosphorylation of Rab11-FIP proteins by the kinase Par1b/MARK2, and the influence of Rab11
Our experimental approach identified rab11a as part of the major complex associated with the C-terminal targeting sequence of rhodopsin in mouse retina. Discounting the mitochondrial ant2 protein as an artifact, all of the proteins detected are known components of outer segment trafficking. A role for rab11a in rhodopsin trafficking has been reported in Drosophila melanogaster (Satoh et al., 2005) and frogs (Mazelova et al., 2009), but the direct interaction of rab11a and, specifically, the C terminus of the rhodopsin molecule has not previously been detected in vertebrate photoreceptors. The fact that this interaction is disrupted by mutations in the C terminus of rhodopsin that lead to defects in outer segment formation and retinal degeneration, including rhodopsin-EGFP and rhodopsinQ344X, suggests that the loss of rab11a binding may be the main cause of these defects.. Two other rab proteins, rab6 (Deretic and Papermaster, 1993; Shetty et al., 1998) and rab8 (Deretic et al., 1995; Moritz et ...
C2 domain second repeat present in Synaptotagmin-like proteins 1-4. All Slp members basically share an N-terminal Slp homology domain (SHD) and C-terminal tandem C2 domains (named the C2A domain and the C2B domain) with the SHD and C2 domains being separated by a linker sequence of various length. Slp1/JFC1 and Slp2/exophilin 4 promote granule docking to the plasma membrane. Additionally, their C2A domains are both Ca2+ independent, unlike the case in Slp3 and Slp4/granuphilin in which their C2A domains are Ca2+ dependent. It is thought that SHD (except for the Slp4-SHD) functions as a specific Rab27A/B-binding domain. In addition to Slps, rabphilin, Noc2, and Munc13-4 also function as Rab27-binding proteins. It has been demonstrated that Slp3 and Slp4/granuphilin promote dense-core vesicle exocytosis. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta ...
Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial and sporadic Parkinsons disease (PD). Elevated kinase activity is associated with LRRK2 toxicity, but the substrates that mediate neurodegeneration remain poorly defined. Given the increasing evidence suggesting a role of LRRK2 in membrane and vesicle trafficking, here we systemically screened Rab GTPases, core regulators of vesicular dynamics, as potential substrates of LRRK2 and investigated the functional consequence of such phosphorylation in cells and in vivo. In vitro LRRK2 kinase assay with forty-five purified human Rab GTPases was performed to identify Rab family proteins as substrates of LRRK2. We identified the phosphorylation site by tandem mass-spectrometry and confirmed it by assessing phosphorylation in the in vitro LRRK2 kinase assay and in cells. Effects of Rab phosphorylation on neurodegeneration were examined in primary cultures and in vivo by intracranial injection of adeno-associated viral
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in
Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
Epithelial cells maintain intercellular communication directly by cell-cell, cell-ECM interaction and indirectly by paracrine secretion of extracellular messengers either as free molecules or via exosomes. The vesicular trafficking machinery is an integral part of such communication, paralleling digital cables that transmit the information bytes to the correct hub. During the process of EMT, derailing of the trafficking machinery leads to a systemic breakdown of intra and inter-cellular communication pathways. The purpose of the current study is to elucidate the functional role of Rab25, a key member of the vesicular trafficking machinery in mediating EMT-MET processes during breast cancer progression.. Aberrant endocytosis is a new hallmark of cancer. Rab GTPases are the primary regulators of endocytosis. Many members of this large family are implicated in a wide array of cancers including breast cancer. Specifically, Rab25, a GTPase associated with the recycling endosomes, is deregulated in ...
The tripartite DENN (after differentially expressed in neoplastic versus normal cells) domain is found in several proteins involved in Rab-mediated processes or regulation of MAPKs (Mitogen-activated preotein kinases) signaling pathways. It actually consists of three parts as the original DENN domain is always encircled on both sides by more divergent domains, called uDENN (after upstream DENN) and dDENN (for downstream DENN). The tripartite DENN domain is found associated with other domains, such as RUN, PLAT, PH, PPR, WD-40, GRAM or C1. The function of DENN domain remains to date unclear, although it appears to represent a good candidate for a GTP/GDP exchange activity [(PUBMED:11563850), (PUBMED:12906859)].. The general characteristics of DENN domains - three regions dDENN, DENN itself, and uDENN having different patterns of sequence conservation and separated by sequences of variable length - suggest that they are composed of at least three sub-domains which may feature distinct folds but ...
1. General Function. Rab1 is a small GTP binding protein that is expressed in virtually all mammalian cells, fish, worms and flies and is homologous to the yeast protein Ypt1 (3). It is essential for ER to Golgi transport and has also been implicated in intra Golgi transport (22, 30). There are two isoforms Rab1a (205 aa) and Rab1b (201aa) which are 92% identical at the amino acid level with most differences in the carboxyl terminus (28). These two isoforms are generally localized in the same cellular regions and have similar biochemical properties and functions. Rab1a may also play a role in transcytosis (14). In addition to localization by immunoflourescence in tissue culture cells, Rab1a has been localized by immunogold labeling to vesicles between the ER and Golgi region and over Golgi stacks in NRK cells (23).. The vesicular transport activity of Rab1 is dependent on its GTPase activity as a GDP bound mutant form, Rab1aS25N and the nucleotide free mutant (N124I) block transport from ER to ...
GCC185, a trans-Golgi network-localized protein predicted to assume a long, coiled-coil structure, is required for Rab9-dependent recycling of mannose 6-phosphate receptors (MPRs) to the Golgi and for microtubule nucleation at the Golgi via CLASP proteins. GCC185 localizes to the Golgi by cooperative interaction with Rab6 and Arl1 GTPases at adjacent sites near its C terminus. We show here by yeast two-hybrid and direct biochemical tests that GCC185 contains at least four additional binding sites for as many as 14 different Rab GTPases across its entire length. A central coiled-coil domain contains a specific Rab9 binding site, and functional assays indicate that this domain is important for MPR recycling to the Golgi complex. N-Terminal coiled-coils are also required for GCC185 function as determined by plasmid rescue after GCC185 depletion by using small interfering RNA in cultured cells. Golgi-Rab binding sites may permit GCC185 to contribute to stacking and lateral interactions of Golgi cisternae as
Background Ypt/Rab GTPases and their GEF activators regulate intra-cellular trafficking in every eukaryotic cells. Furthermore, the primary, aswell as the forecasted secondary, structure from the Trs120- and Trs130-related sequences are conserved from fungi to pets. The mammalian orthologs of Trs130 and Trs120, TMEM1 and NIBP, respectively, are applicants for individual disorders. Presently, NIBP is normally implicated in signaling, and TMEM1 is normally suggested to possess trans-membrane domains (TMDs) also to work as a membrane route. However, we present here which the yeast Trs130 will not work as a trans-membrane proteins, and the individual TMEM1 will not contain putative TMDs. The nonessential subunit, Trs65, is normally conserved just among many fungi plus some unicellular eukaryotes. Multiple position analysis of every TRAPPII-specific subunit uncovered conserved domains including highly conserved proteins. Conclusion We claim that the function of both NIBP and TMEM1 in the legislation ...
Subcellular distribution of wild-type and mutant Rab24 expressed in 293 cells. (A) Cells were transfected with vectors encoding mycRab24wt or mycRab24(D123I) an
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Strict spatiotemporal control of trafficking events between organelles is critical for maintaining homeostasis and directing cellular responses. This regulation is particularly important in immune cells for mounting specialized immune defences. By controlling the formation, transport and fusion of intracellular organelles, Rab GTPases serve as master regulators of membrane trafficking. In this review, we discuss the cellular and molecular mechanisms by which Rab GTPases regulate immunity and inflammation.
Rab24 is a member of the Rab GTPase family, but its function is unclear. Here, we demonstrated increase in Rab24 mRNA in nerve… Expand ...
TY - JOUR. T1 - Rab1 interacts directly with the β2-adrenergic receptor to regulate receptor anterograde trafficking. AU - Hammad, Maha. AU - Kuang, Yi Qun. AU - Morse, Alexa. AU - Dupré, Denis J.. PY - 2012/5/1. Y1 - 2012/5/1. N2 - Very little is understood about the trafficking of G protein-coupled receptors (GPCRs) from the endoplasmic reticulum (ER) to the plasma membrane. Rab guanosine triphosphatases (GTPases) are known to participate in the trafficking of various GPCRs via a direct interaction during the endocytic pathway, but whether this occurs in the anterograde pathway is unknown. We evaluated the potential interaction of Rab1, a GTPase known to regulate β2-adrenergic receptor (β2AR) trafficking, and its effect on export from the ER. Our results show that GTP-bound Rab1 interacts with the F(x) 6LL motif of β2AR. Receptors lacking the interaction motif fail to traffic properly, suggesting that a direct interaction with Rab1 is required for β2AR anterograde trafficking.. AB - Very ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a GTPase-activating protein that activates the small guanine-nucleotide-binding protein Rap1 in platelets. The protein interacts with synaptotagmin-like protein 1 and Rab27 and regulates secretion of dense granules from platelets at sites of endothelial damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009 ...
In mammalian cells vesicle transport is an important process, many diseases are caused by defects of trafficking. Key elements for organelle trafficking are Rab proteins which regulate this system. Rabs are GTPases and belong to the Ras superfamily proteins. Rab27a is one of the main actors of this process, it promotes the recruitment of secretory vesicles at the release site, the plasma membrane, by its role of interconnection between the vesicle and the motor protein myosin V. Dysfunction leads to disease such as Griscelli syndrome. Rab27a is a molecular switch, in the active state is able to bind effectors. The switching from the GDP-bound to the GTP-bound is catalysed by Rab3GEP. Furthermore, to function properly Rab27a has to be targeted to the organelle membrane, this function has been attributed to the guanine exchange factor as well. Rab3GEP contains a DENN domain (differentially expressed in normal versus neoplastic) at the N-terminus and a death domain at the C-terminus. Rab3GEP ...
Rab proteins comprise a family of monomeric GTPases that control cellular membrane traffic. Rab21 is a poorly characterised member with no known function. Human Rab21 cDNA from K562 cells was subcloned into GFP expression vectors to generate Rab21 and Rab21 mutants defective in either GTP hydrolysis (Rab21 Q78L) or binding (Rab21 T33N) for transfection studies in HeLa cells. Confocal fluorescence microscopy and ultrastructural studies revealed Rab21 to be predominantly localised to the early endocytic pathway, on vesicles containing earlyendosomal antigen 1 EEA1, transferrin receptor and internalised ligands. EEA1 was localised to enlarged endosomes in Rab21 wild-type expressing cells but the GTP hydrolysis and GDP binding mutants had unique phenotypes labelling tubular reticular structures and the trans-Golgi network, respectively. Early endosome localisation for Rab21 was confirmed in a hepatoma cell line that allowed analysis of the subcellular distribution of the endogenous protein. ...
Rab proteins are small GTPases that control the direction and timing of vesicle fusion during intracellular trafficking between membraneous compartments. Genome sequencing and EST analysis of Trypanosoma brucei indicates that the trypanosome Rab (TbRAB) gene family, and hence complexity of intracellular transport pathways, is intermediate between Saccharomyces cerevisiae and mammals. TbRAB31 is a constitutively expressed T. brucei Rab protein (formerly Trab7p) and is the product of one of two closely linked TbRAB genes, the other being TbRAB2 (TbRab2p, in: Field H, Ali BRS, Sherwin T, Gull K, Croft SL, Field MC. TbRab2p, a marker for the endoplasmic reticulum of Trypanosoma brucei, localises to the ERGIC in mammalian cells. J Cell Sci 1999; 112:147-156), involved in ER to Golgi transport. TbRAB31 has high homology to members of the Sec4/Ypt1 subfamily of Rab proteins from S. cerevisiae and to Rab13 and Rab11 from higher eukaryotes. Recombinant TbRAB31 binds GTP but, unusually for a Rab protein, has
A prequel to endosomal maturation into late endosomal/lysosomal organelles is Rab conversion (Rink et al., 2005). This term describes a process whereby an organelle synchronously sheds off early endosomal Rab(s) and concomitantly receives the late endosomal Rab, Rab7 (Rink et al., 2005). The signals for this transition are currently unknown (Deretic, 2005). We wondered whether Rab conversion applies to phagosomes, and whether Rab22a, as a candidate terminal recycling Rab involved in cargo and membrane sorting from the early endosome (Mesa et al., 2001; Weigert et al., 2004), could supply or contribute to such signals. To test this, we examined Rab7 acquisition by the mycobacterial phagosome, which was previously shown to exclude this critical late endocytic Rab (Via et al., 1997). Unlike in cells treated with control scrambled siRNA, Rab7 acquisition was increased to 80% on live mycobacterial phagosomes in macrophages in which Rab22a was knocked down by siRNA (Fig. 5). These findings are ...
Rab GTPases function as modulators in intracellular transport. Rab5a, a member of the Rab subfamily of small GTPases, is an important regulator of vesicle traffic from the plasma membrane to early endosomes. Recent findings have reported that Rab5a gene was involved in the progression of cancer. In the present study, we investigated the effect of Rab5a on cervical cancer invasion and metastasis and the molecular mechanism underlying the involvement of Rab5a. Rab5a expression was assessed by immunohistochemical analysis on a cervical cancer tissue microarray. RNA interference (RNAi) was performed to knock down the endogenous expression of Rab5a gene in HeLa and SiHa cells. Cell motility was evaluated using invasion assay and wound migration assay in vitro. The expression levels of integrin-associated molecules were detected by Western blot and immunofluorescence. We found that Rab5a was expressed at a high level in cervical cancer tissues. Silencing of Rab5a expression significantly decreased cancer cell
TY - JOUR. T1 - Warburg Micro syndrome is caused by RAB18 deficiency or dysregulation. AU - Handley, Mark T.. AU - Carpanini, Sarah. AU - Mali, Girish. AU - Sidjanin, Duska J.. AU - Aligianis, Irene A.. AU - Jackson, Ian J.. AU - Fitzpatrick, David R.. N1 - MRC. PY - 2015/6/1. Y1 - 2015/6/1. N2 - RAB18, RAB3GAP1, RAB3GAP2 and TBC1D20 are each mutated in Warburg Micro syndrome, a rare autosomal recessive multisystem disorder. RAB3GAP1 and RAB3GAP2 form a binary RAB3GAP complex that functions as a guanine-nucleotide exchange factor (GEF) for RAB18, whereas TBC1D20 shows modest RAB18 GTPase-activating (GAP) activity in vitro. Here, we show that in the absence of functional RAB3GAP or TBC1D20, the level, localization and dynamics of cellular RAB18 is altered. In cell lines where TBC1D20 is absent from the endoplasmic reticulum (ER), RAB18 becomes more stably ER-associated and less cytosolic than in control cells. These data suggest that RAB18 is a physiological substrate of TBC1D20 and contribute ...
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Rab GTPases serve as molecular switches to regulate eukaryotic membrane trafficking pathways. The transport protein particle (TRAPP) complexes activate Rab GTPases by catalyzing GDP/GTP nucleotide exchange. In mammalian cells, there are two distinct TRAPP complexes, yet in budding yeast, four distinct TRAPP complexes have been reported. The apparent differences between the compositions of yeast and mammalian TRAPP complexes have prevented a clear understanding of the specific functions of TRAPP complexes in all cell types. In this study, we demonstrate that akin to mammalian cells, wild-type yeast possess only two TRAPP complexes, TRAPPII and TRAPPIII. We find that TRAPPIII plays a major role in regulating Rab activation and trafficking at the Golgi in addition to its established role in autophagy. These disparate pathways share a common regulatory GTPase Ypt1 (Rab1) that is activated by TRAPPIII. Our findings lead to a simple yet comprehensive model for TRAPPIII function in both normal and ...
Rab1 is a small GTPase regulating vesicular traffic between early compartments of the secretory pathway. To explore the role of rab1 we have analyzed the function of a mutant (rab1a[S25N]) containing a substitution which perturbs Mg2+ coordination and reduces the affinity for GTP, resulting in a form which is likely to be restricted to the GDP-bound state. The rab1a(S25N) mutant led to a marked reduction in protein export from the ER in vivo and in vitro, indicating that a guanine nucleotide exchange protein (GEP) is critical for the recruitment of rab1 during vesicle budding. The mutant protein required posttranslational isoprenylation for inhibition and behaved as a competitive inhibitor of wild-type rab1 function. Both rab1a and rab1b (92% identity) were able to antagonize the inhibitory activity of the rab1a(S25N) mutant, suggesting that these two isoforms are functionally interchangeable. The rab1 mutant also inhibited transport between Golgi compartments and resulted in an apparent loss of ...
The ~200 amino acid TBC/rab GTPase-activating protein (GAP) domain is well conserved across species and has been found in a wide range of different proteins from plant adhesion molecules to mammalian oncogenes. The name TBC derives from the name of the murine protein Tbc1 in which this domain was first identified based on its similarity to sequences in the tre-2 oncogene, and the yeast regulators of mitosis, BUB2 and cdc16 [ (PUBMED:7566974) ]. The connection of this domain with rab GTPase activation stems from subsequent in-depth sequence analyses and alignments [ (PUBMED:9255064) ] and recent work demonstrating that it appears to contain the catalytic activities of the yeast rab GAPs, GYP1, and GYP7 [ (PUBMED:10508155) ]. The TBC/rab GAP domain has also been named PTM after three proteins known to contain it: the Drosophila pollux, the human oncoprotein TRE17 (oncoTRE17), and a myeloid cell line-expressed protein [ (PUBMED:8654926) ]. The TBC/rab GAP domain contains six conserved motifs named ...
Ras-related protein Rab-11A is a protein that in humans is encoded by the RAB11A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Rab-11a controls intracellular trafficking of the innate immune receptor TLR4, and thereby also receptor signalling RAB11A has been shown to interact with: RAB11FIP1, RAB11FIP2, RAB11FIP3, RAB11FIP4, and RAB11FIP5 GRCh38: Ensembl release 89: ENSG00000103769 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000004771 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Drivas GT, Shih A, Coutavas EE, DEustachio P, Rush MG (Jan 1991). Identification and characterization of a human homolog of the Schizosaccharomyces pombe ras-like gene YPT-3. Oncogene. 6 (1): 3-9. PMID 1704119. Gromov PS, Celis JE, Hansen C, Tommerup N, Gromova I, Madsen P (Jun 1998). Human rab11a: transcription, ...
The stiochiometric phosphorylation of golgin-84 in mitosis together with its binding to active rab1 suggested that it may play a role in Golgi structure and/or membrane trafficking through the Golgi apparatus. To address a possible structural role for golgin-84, GFP-tagged full-length and truncated versions of the protein were expressed in HeLa cells, and effects upon Golgi structure were analyzed by immunofluorescence microscopy (Fig. 5 a). At moderate expression levels, none of the golgin-84 constructs elicited any significant change in Golgi structure (unpublished data). Furthermore, constructs that failed to target to the Golgi apparatus had no effects upon Golgi structure even at very high levels of expression (Fig. 5 a; unpublished data). In contrast, expression at high levels of both full-length and golgin-84 lacking the head region had dramatic effects upon Golgi structure, converting the ribbon into punctate structures dispersed throughout the cytoplasm (Fig. 5 b). EM analysis of the ...
RAB6B Human - Recombinant Human RAB6B, Member RAS Oncogene Family (5ug) - Read User Reviews, Features, Research Applications and get the Best Price/Quote
Expression vectors. Expression vectors for wild-type and mutant Rab proteins were constructed by subcloning their cDNAs into pcDNA3 or pCMV5 mammalian expression vectors. Rab15 expression vector was kindly provided by Dr. L. Elferink (Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, TX). The hGH expression vector pHGHCMV5 was described previously (Sugita et al., 1999). To generate N-terminal green fluorescent protein (GFP) fusion constructs, wild-type and mutant Rab11a, Rab11b, and Rab25 cDNAs were amplified by PCR and subcloned into pEYFP-N1 expression vector (Clontech, Palo Alto, CA). All expression constructs were verified by sequencing.. Antibodies. Glutathione S-transferase (GST)-fused recombinant Rab11b was expressed in bacteria, purified on a glutathione agarose column, and digested with thrombin to remove GST part. Purified recombinant protein was then used as an antigen to produce polyclonal rabbit antibodies against Rab11b (T3746). Antibodies were ...
TY - JOUR. T1 - Vps9 domain-containing proteins. T2 - Activators of Rab5 GTPases from yeast to neurons. AU - Carney, Darren S.. AU - Davies, Brian A.. AU - Horazdovsky, Bruce F.. PY - 2006/1/1. Y1 - 2006/1/1. N2 - Endocytosis of cell surface receptors plays an important role in regulating cell signaling cascades. In some cases, internalization of an activated receptor attenuates the signaling process, while in other cases the clustering of activated receptors on early endosomal structures has been proposed to be essential for fully activating signaling cascades. Regulating the movement of receptors and other signaling proteins through the endocytic pathway, therefore, has a direct impact on cellular homeostasis. The small GTPase Rab5 is a crucial regulatory component of the endocytic pathway. Activation of Rab5 is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex. A large number of proteins have been identified that contain a specific, highly conserved domain (Vps9) ...
Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells..[Nature Cell Biol.,14(8),(2012),838-849]Gálvez-Santisteban, M., Rodriguez-Fraticelli, A. E., Bryant, D. M., Vergarajauregui, S., Yasuda, T., Bañón-Rodríguez, I., Bernascone, I., Datta, A., Spivak, N., Young, K., Slim, C. L., Brakeman, P. R., Fukuda, M., Mostov, K. E. and Martín-Belmonte, F ...
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Mouse monoclonal antibody raised against a partial recombinant RABGAP1. RABGAP1 (AAH54492, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00023637-M01) - Products - Abnova
A RIKEN-led team of molecular biologists has determined the specific bonding leading to the formation of a protein complex involved in distributing pigment throughout the skin. Disruption of this membrane transport complex leads to the rare, lethal Griscelli syndrome for which there is no effective treatment. Patients show symptoms of albinism, suffer immunodeficiency, and typically die in early childhood. The work may stimulate the development of therapeutic drugs for the condition.. Members of the Rab protein family-of which there are more than 60 in humans-are thought to be essential to membrane trafficking, an important form of communication and distribution within cells. Rab proteins are typically bound to membranes either in an inactive guanine diphosphate form or an active triphosphate form which works through specific effector molecules to promote membrane trafficking.. The pigment melanin, which protects against radiation damage, is made and distributed within vesicles called ...
Ras-related protein Rab-11A; The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. T [...] (216 aa ...
Mouse monoclonal antibody raised against full length recombinant human RAB27A. Recombinant protein corresponding to full length human RAB27A. (MAB12243) - Products - Abnova
critical interest to MS research. Results presented in this study demonstrate that an increase of Rab32 in the inflamed brain directly promotes neuronal cell death from a combination of apoptosis and necroptosis. Interestingly, the putative role of Rab32 as an autophagy promoter [38] is not tied to this pro-death function of Rab32. While wild-type and active Rab32Q85L showed effects on mitochondrial morphology and neurite outgrowth, inactive Rab32T39N also compromised the survival of primary neurons as well as SH-SY5Y cells (Figs. 5 and 6), suggesting the mere upregulation of Rab32 is detrimental to neuronal function, potentially due to shared functions of active and inactive Rab32. Moreover, our results reinforce the role of ER stress as an upstream trigger of inflammation, which is one of the main pathology drivers in the MS context. Interestingly, the inhibition of the UPR can improve myelination of some disease models [39] and also due to shared functions of active and inactive Rab32. ...
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RAB27A KO cell line available now. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 3 and 1 bp deletion in exon 3.
Blog on RAB31 sirna product: The RAB31 rab31 (Catalog #MBS8217875) is a siRNA produced from Synthetic and is intended for research pur...
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Complete information for DENND5B gene (Protein Coding), DENN Domain Containing 5B, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for DENND5B gene (Protein Coding), DENN Domain Containing 5B, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The structure and function of the primary cilium as a sensory organelle depends on a motor-protein-powered intraflagellar transport system (IFT); defective IFT results in retinal degeneration and pleiotropic disorders such as the Bardet Biedl syndrome (BBS) and defective hedgehog (HH) signaling. Pro …
RAB23 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 254 amino acids (1-234) and having a molecular mass of 28.5 kDa.RAB23 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
RAB13 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (1-200) and having a molecular mass of 24.7 kDa.The RAB13 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Pereira-Leal JB, Seabra MC (Nov 2001). "Evolution of the Rab family of small GTP-binding proteins". J Mol Biol. 313 (4): 889- ... Ras-related protein Rab-15 is a protein that in humans is encoded by the RAB15 gene. GRCh38: Ensembl release 89: ... Fukuda M (2003). "Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant ... Strick DJ, Elferink LA (2006). "Rab15 effector protein: a novel protein for receptor recycling from the endocytic recycling ...
Pereira-Leal JB, Seabra MC (Nov 2001). "Evolution of the Rab family of small GTP-binding proteins". J Mol Biol. 313 (4): 889- ... Ras-related protein Rab-40A is a protein that in humans is encoded by the RAB40A gene. GRCh38: Ensembl release 89: ... domain-containing proteins based on their Rab-binding activity". Genes Cells. 11 (9): 1023-37. doi:10.1111/j.1365-2443.2006. ... Fukuda M (2003). "Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant ...
Pereira-Leal JB, Seabra MC (Nov 2001). "Evolution of the Rab family of small GTP-binding proteins". J Mol Biol. 313 (4): 889- ... Ras-related protein Rab-21 is a protein that in humans is encoded by the RAB21 gene. GRCh38: Ensembl release 89: ... Rab21 is an apically located GTP-binding protein in polarised intestinal epithelial cells". Eur J Cell Biol. 79 (5): 308-16. ... Opdam FJ, Kamps G, Croes H, van Bokhoven H, Ginsel LA, Fransen JA (Oct 2000). "Expression of Rab small GTPases in epithelial ...
Pereira-Leal JB, Seabra MC (November 2001). "Evolution of the Rab family of small GTP-binding proteins". J Mol Biol. 313 (4): ... Ras-related protein Rab-25 is a protein that in humans is encoded by the RAB25 gene. It is thought to act as a promoter of ... Goldenring JR, Shen KR, Vaughan HD, Modlin IM (1993). "Identification of a small GTP-binding protein, Rab25, expressed in the ... 1997). "Increased immunoreactivity for Rab11, a small GTP-binding protein, in low-grade dysplastic Barrett's epithelia". Lab. ...
Zahraoui A, Touchot N, Chardin P, Tavitian A (Jul 1989). "The human Rab genes encode a family of GTP-binding proteins related ... Karniguian A, Zahraoui A, Tavitian A (Aug 1993). "Identification of small GTP-binding rab proteins in human platelets: thrombin ... "Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2". Nature. 350 (6320): 715-8. doi:10.1038/ ... Ras-related protein Rab-1A is a protein that in humans is encoded by the RAB1A gene. RAB1A has been shown to interact with: ...
Zahraoui A, Touchot N, Chardin P, Tavitian A (Jul 1989). "The human Rab genes encode a family of GTP-binding proteins related ... Karniguian A, Zahraoui A, Tavitian A (Aug 1993). "Identification of small GTP-binding rab proteins in human platelets: thrombin ... "Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2". Nature. 350 (6320): 715-8. doi:10.1038/ ... "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5". The EMBO Journal. 17 (7): ...
Mss4 stimulates GTP-GDP exchange in Sec4 and Rab and binds to a subset of genetically related Rab proteins. GRCh38: Ensembl ... The Sec4/Rab-related small GTP-binding proteins are involved in the regulation of intracellular vesicular transport. ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... "Entrez Gene: RABIF RAB interacting factor". Yu H, Schreiber SL (1995). "Structure of guanine-nucleotide-exchange factor human ...
Zahraoui A, Touchot N, Chardin P, Tavitian A (1989). "The human Rab genes encode a family of GTP-binding proteins related to ... Karniguian A, Zahraoui A, Tavitian A (1993). "Identification of small GTP-binding rab proteins in human platelets: thrombin- ... Ras-related protein Rab-6A is a protein that in humans is encoded by the RAB6A gene located in the eleventh chromosome. Its ... Goud B, Zahraoui A, Tavitian A, Saraste J (1990). "Small GTP-binding protein associated with Golgi cisternae". Nature. 345 ( ...
The mammalian RAB proteins show striking similarities to the S. cerevisiae YPT1 and SEC4 proteins, Ras-related GTP-binding ... Members of the Rab protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 ... Zahraoui A, Touchot N, Chardin P, Tavitian A (Jul 1989). "The human Rab genes encode a family of GTP-binding proteins related ... Ras-related protein Rab-2A is a protein that in humans is encoded by the RAB2A gene. ...
Zahraoui A, Touchot N, Chardin P, Tavitian A (July 1989). "The human Rab genes encode a family of GTP-binding proteins related ... Ras-related protein Rab-3B is a protein that in humans is encoded by the RAB3B gene. RAB3B has been shown to interact with ... "Entrez Gene: RAB3B RAB3B, member RAS oncogene family". Fukuda M (April 2003). "Distinct Rab binding specificity of Rim1, Rim2, ... Fukuda M (April 2003). "Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical ...
Zahraoui A, Touchot N, Chardin P, Tavitian A (Jul 1989). "The human Rab genes encode a family of GTP-binding proteins related ... "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5". The EMBO Journal. 17 (7): ... Ras-related protein Rab-5A is a protein that in humans is encoded by the RAB5A gene. RAB5A localizes to early endosomes where ... Cremers FP, Armstrong SA, Seabra MC, Brown MS, Goldstein JL (Jan 1994). "REP-2, a Rab escort protein encoded by the ...
GDI1: RabGDI alpha makes a complex with geranylgeranylated small GTP-binding proteins of the Rab family and keeps them in the ... IQSEC2: encodes an exchange factor for the Arf family of small GTP binding proteins, involved in the formation of secretory ... FTSJ1: Transfert RNA methyltransferase 1 is a member of the S-adenosylmethionine-binding protein family. This nucleolar protein ... The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. PHF8: PHD ...
The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding ... This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may ... Majidi M, Hubbs AE, Lichy JH (June 1998). "Activation of extracellular signal-regulated kinase 2 by a novel Abl-binding protein ... Suppression of tumorigenicity 5 is a protein that in humans is encoded by the ST5 gene. ST5 orthologs have been identified in ...
Members of the RAB family of RAS-related GTP-binding proteins are important regulators of vesicular transport and are located ... Ras-related protein Rab-7a is a protein that in humans is encoded by the RAB7A gene. Ras-related protein Rab-7a is involved in ... These proteins are GTPases and act like switch which is turned on and off by GTP and GDP molecules. Melanoma cells retain a ... The GTP-bound form interacts with OSBPL1A and CLN3. Rab7A was also shown to interact with the Retromer Complex, most likely ...
... encoding a small GTP-binding protein homologous to the Ras-related RAB gene". Cytogenet Cell Genet. 73 (1-2): 137-9. doi: ... Ras-related protein Rab-5C is a protein that in humans is encoded by the RAB5C gene. RAB5C belongs to the Ras-related protein ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... 1999). "Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)". Biochem. ...
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP- ... GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously ... Rab GDP-dissociation inhibitor beta and histidine triad nucleotide-binding protein in fetal Down syndrome brain". J. Neural ... Rab GDP dissociation inhibitor beta is a protein that in humans is encoded by the GDI2 gene. ...
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP- ... Rab GTPases cycles between the cytosolic compartment, where it is bound to a protein called GDI (GDP Dissociation Inhibitor), ... GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed ... GDI is non-specific with respect to the rab it binds. However, the exchanger, receptor and GAP, are rab specific. GDI1 has been ...
... in bone-resorbing osteoclasts revealed by cloning and subcellular localization studies of small GTP-binding rab proteins". ... Ras-related protein Rab-1B is a protein that in humans is encoded by the RAB1B gene. RAB1B has been shown to interact with ... 2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. It is GTP-binding protein and may be ... Koda T, Kakinuma M (Sep 1993). "Molecular cloning of a cDNA encoding a novel small GTP-binding protein". FEBS Lett. 328 (1-2): ... Ras-related protein Rab-33A is a protein that in humans is encoded by the RAB33A gene. ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ...
Regulatory Rab proteins are thought to inspect the joining of the SNAREs. Rab protein is a regulatory GTP-binding protein and ... controls the binding of these complementary SNAREs for a long enough time for the Rab protein to hydrolyse its bound GTP and ... Membrane-bound and secreted proteins are made on ribosomes found in the rough endoplasmic reticulum. Most of these proteins ... Coat proteins can also function to bind to various transmembrane receptor proteins, called cargo receptors. These receptors ...
... bind to the GDP-bound form of Rho and Rab small GTPases and not only prevent exchange (maintaining the small GTPase in an off- ... GTP-binding protein regulators regulate G proteins in several different ways. Small GTPases act as molecular switches in ... between the GTP-bound and GDP-bound form, regulated by other regulatory proteins. ... They are active or 'ON' when it is bound to GTP and inactive or 'OFF' when bound to GDP.[1] Activation and deactivation of ...
The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab ... The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. ... Strom M, Hume AN, Tarafder AK, Barkagianni E, Seabra MC (July 2002). "A family of Rab27-binding proteins. Melanophilin links ... Nalefski EA, Falke JJ (December 1996). "The C2 domain calcium-binding motif: structural and functional diversity". Protein ...
... allowing for Rab protein to change from its GDP to GTP bound state. Inhibition of the GTPase-activating domain leaves proteins ... Two proteins with capacity to bind Ubc9 and conjugated to SUMO1". The Journal of Biological Chemistry. 277 (22): 19783-91. doi: ... Like all proteins, the unique amino acid arrangement in the primary sequence of GLUT4 is what allows it to transport glucose ... GLUT4+Protein at the US National Library of Medicine Medical Subject Headings (MeSH) USCD-Nature molecule pages: The signaling ...
The cycle between the GTP-bound inactive state and the GTP- bound active state is led by the Rab protein and regulated by an ... Hereafter, the activated form of Rab, GTP-bound, is incorporated to a specific organelle or vesicle and promotes its transport ... domain-containing proteins based on their Rab-binding activity". Genes to Cells. 11 (9): 1023-37. doi:10.1111/j.1365-2443.2006. ... GTPase-activating proteins (GAPs) limit the duration of the active state and accelerate the slow intrinsic rate of GTP ...
The protein encoded by this gene is a member of the RAB family of small GTPases. The GTP-bound form of the encoded protein has ... 2000). "Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in ... Ras-related protein Rab-22A is a protein that in humans is encoded by the RAB22A gene. ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-1178. doi: ...
A typical G-protein is active when bound to GTP and inactive when bound to GDP (i.e. when the GTP is hydrolyzed to GDP). The ... Based on structure, sequence and function, the Ras superfamily is divided into five main families, (Ras, Rho, Ran, Rab and Arf ... GTP-binding protein regulators Wennerberg K, Rossman KL, Der CJ (March 2005). "The Ras superfamily at a glance". J. Cell Sci. ... Therefore, a G-protein can be switched on and off. GTP hydrolysis is accelerated by GTPase activating proteins (GAPs), while ...
Ras related protein), which is also the substrate of Rab1b GEF domain. Rab1b protein is the GTPase Rab to regulate vesicle ... The adenylation by bacteria AMPylators prolong GTP-bound state of Rab1b. Thus, the role of effector DrrA is connected toward ... This family of proteins are found in all domains of life on earth. It is mediated via a mechanism of ATP-binding-site alpha ... PII is the trimeric protein. There are two forms of PII, including unmodified PII and PII - UMP. PII binds to AMPylator ...
The protein forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin ... Fukuda M (2003). "Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant ... Strom M, Hume AN, Tarafder AK, Barkagianni E, Seabra MC (July 2002). "A family of Rab27-binding proteins. Melanophilin links ... Fukuda M (2002). "Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a/melanophilin is a critical determinant of GTP- ...
The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a ... The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average ... and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. ... the GTP-binding protein rab8 interacts with a stress-activated protein kinase". Proceedings of the National Academy of Sciences ...
Deficiencies of intracellular signaling peptides and proteins. GTP-binding protein regulators. GTPase-activating protein. * ... Mutations in the RPS6KA3 disturb the function of the protein, but it is unclear how a lack of this protein causes the signs and ... The RPS6KA3 gene makes a protein that is involved with signaling within cells. Researchers believe that this protein helps ... The protein RSK2 which is encoded by the RPS6KA3 gene is a kinase which phosphorylates some substrates like CREB and histone H3 ...
Rouaux C, Loeffler JP, Boutillier AL (September 2004). "Targeting CREB-binding protein (CBP) loss of function as a therapeutic ... "Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors". primary. The Journal of Cell Biology. 191 (2): ... The UBQLN2 gene encodes the protein ubiquilin 2 which is responsible for controlling the degradation of ubiquitinated proteins ... As that gene's name suggests, BACE1 is an enzymatic protein that cleaves the Amyloid Precursor Protein into the insoluble ...
Deficiencies of intracellular signaling peptides and proteins. GTP-binding protein regulators. GTPase-activating protein. * ... RAB: RAB7 *Charcot-Marie-Tooth disease. *RAB23 *Carpenter syndrome. *RAB27 *Griscelli syndrome type 2 ...
"The small GTP-binding protein, Rhes, regulates signal transduction from G protein-coupled receptors". Oncogene. 23 (2): 559-68 ... Luks VL, Kamitaki N, Vivero MP, Uller W, Rab R, Bovée JV, Rialon KL, Guevara CJ, Alomari AI, Greene AK, Fishman SJ, Kozakewich ... protein serine/threonine kinase activity. • GO:0001948 protein binding. • insulin receptor substrate binding. • ATP binding. • ... nucleotide binding. • protein kinase activator activity. • 1-phosphatidylinositol-4-phosphate 3-kinase activity. • ...
protein binding. • thioesterase binding. • protein kinase binding. • nucleotide binding. • GTP binding. • identical protein ... ubiquitin protein ligase activity. • apolipoprotein A-I receptor binding. • GTP-dependent protein binding. • GTPase activity. • ... "Protein Data Bank in Europe. EMBL-EBI. Retrieved 2016-04-22.. *^ "CDC42 (cell division cycle 42 (GTP binding protein, 25kDa))" ... which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase GTP hydrolysis activity ...
nucleotide binding. • GTP binding. • myosin V binding. • GO:0001948 protein binding. • GO:0006184 GTPase activity. ... Protein sy'n cael ei godio yn y corff dynol gan y genyn RAB6B yw RAB6B a elwir hefyd yn Ras-related protein Rab-6B a RAB6B, ... intracellular protein transport. • Rab protein signal transduction. • small GTPase mediated signal transduction. ... "Rab6 family proteins interact with the dynein light chain protein DYNLRB1.". Cell Motil Cytoskeleton. 2008. PMID 18044744. ...
"Regulator of G protein signalling"). Receptors stimulate GTP binding (turning the G protein on). RGS proteins stimulate GTP ... Wikimedia Commons has media related to G proteins.. *GTP-Binding Proteins at the US National Library of Medicine Medical ... G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside ... They are small (20-kDa to 25-kDa) proteins that bind to guanosine triphosphate (GTP). This family of proteins is homologous to ...
Deficiencies of intracellular signaling peptides and proteins. GTP-binding protein regulators. GTPase-activating protein. * ... The mutation can appear in GJB1 coding for connexin 32, a gap junction protein expressed in Schwann cells. Because this protein ... Some mutations affect the gene MFN2, on chromosome 1, which codes for a mitochondrial protein. Mutated MFN2 causes the ... Cell membrane protein disorders (other than Cell surface receptor, enzymes, and cytoskeleton) ...
G protein signaling is terminated by hydrolysis of bound GTP to bound GDP.[2][3] This can occur through the intrinsic GTPase ... Rab, Arf and Ran.[15] While many small GTPases are activated by their GEFs in response to intracellular signals emanating from ... bound to GTP) only for a short time before deactivating themselves by converting bound GTP to bound GDP.[2][3] However, many ... Artificial GTP analogues like GTP-γ-S, β,γ-methylene-GTP, and β,γ-imino-GTP that cannot be hydrolyzed can lock the GTPase in ...
GO:0001948 protein binding. • thioesterase binding. • protein kinase binding. • nucleotide binding. • GTP binding. • Rab GTPase ... histone deacetylase binding. • Rho GDP-dissociation inhibitor binding. • GTP-dependent protein binding. • GTPase activity. • ... RAC1, MIG5, Rac-1, TC-25, p21-Rac1, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), ... protein phosphorylation. • Rho protein signal transduction. • regulation of lamellipodium assembly. • Rac protein signal ...
Deficiencies of intracellular signaling peptides and proteins. GTP-binding protein regulators. GTPase-activating protein. * ... Mutated p53 proteins are typically more stable than wild-type, and can inhibit the activity of the wild-type protein in ... The tetramerization domain plays a major role in the oligomerization of the p53 protein, which exists as a tetramer.[6] This ... With pH in the low to normal physiological range (up to 7.5), the mutant protein forms normal oligomers and retains its ...
enzyme binding. • protein binding. • thioesterase binding. • protein kinase binding. • nucleotide binding. • GTP binding. • Rab ... histone deacetylase binding. • Rho GDP-dissociation inhibitor binding. • GTP-dependent protein binding. • GTPase activity. • ... "Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac". The Biochemical Journal. 322 (3): 873-8. doi: ... "Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1". Structure. 8 (1): 47-55. doi:10.1016/ ...
Hall, A. (1994). „Small GTP-binding proteins and the regulation of actin cytoskeleton". Annu. Rev. Cell Biol. 10: 31-54. PMID ... Ras · Rab (Rab27) · Arf (Arf6) · Ran · Rheb · Ro familija (RhoA, RhoB, CDC42, Rac1) · Rap ... Tipični G-protein je aktivan kada je vezan za GTP i neaktivan kad je vezan za GDP (i.e. kad je GTP hidrolizovan do GDP). GDP ... look at the regulation of Ras-related GTP-binding proteins". Curr. Opin. Struct. Biol. 7: 786-792. PMID 9434896.. ...
nucleotide binding. • GTP binding. • protein binding. • GTPase activity. Cellular component. • cytoplasm. • perinuclear region ... Compton SL, Kemppainen RJ, Behrend EN (Dec 2009). "Prenylated Rab acceptor domain family member 1 is involved in stimulated ... RASD1 shares several motifs characteristic of Ras proteins, including four highly conserved GTP binding pocket domains: the ... Dexamethasone-induced Ras-related protein 1 (RASD1) is a protein that in humans is encoded by the RASD1 gene on chromosome 17.[ ...
DEPDC1 encoding protein DEP domain containing 1. *DIRAS3 (1p31): DIRAS family, GTP-binding RAS-like 3 ... ARID4B: encoding protein AT-rich interactive domain-containing protein 4B. *ARV1 encoding protein ARV1 homolog (S. cerevisiae) ... RABIF: RAB interacting factor. *RASSF5 (1q32). *RGS2 (1q31). *[email protected]: RNA, 5S ribosomal 1q42 cluster ... So CCDS's gene number prediction represents a lower bound on the total number of human protein-coding genes.[7] ...
GDP binding. • GO:0032403 macromolecular complex binding. • GTP binding. • GMP binding. • GO:0001948 protein binding. • GTPase ... to a molecule of GTP. The K-Ras protein is turned off (inactivated) when it converts the GTP to GDP. When the protein is bound ... it is unlikely that drug-like small molecule inhibitors could compete with GTP/GDP binding. Other than where GTP/GDP binds, ... identical protein binding. Cellular component. • cytoplasm. • cytosol. • membrane. • focal adhesion. • extrinsic component of ...
Rab guanyl-nucleotide exchange factor activity. • identical protein binding. • macromolecular complex binding. • cadherin ... "Phosphorylation-dependent association of the Ras-related GTP-binding protein Rem with 14-3-3 proteins". Arch. Biochem. Biophys ... protein binding. • MHC class II protein complex binding. • protein heterodimerization activity. • enzyme binding. • poly(A) RNA ... ion channel binding. • protein domain specific binding. • protein complex binding. • histone deacetylase binding. • potassium ...
Regulator G protein signalizacije: RGS1, RGS2, RGS3, RGS4, RGS5, RGS6, RGS7, RGS8, RGS9, RGS10, RGS11, RGS12, RGS13, RGS14, ... se vezuje za GDP-vezanu formu Rho i Rab malih GTPaza i ne samo sprečava razmenu (održavajući male GTPaze u isključenom stanju ... Oni su aktivni kad su vezani za GTP i neaktivni kad su vezani za GDP.[1] Aktivacija i deaktivacija malih GTPaza se odvija u ... GTP-vezujući proteinski regulatori regulišu G proteine na nekoliko različitih načina. Male GTPaze deluju kao molekulski ...
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be ... One is the rap1/smg21/Krev-1 protein and the other is a novel GTP-binding protein". J. Biol. Chem. 264 (29): 17000-5. PMID ... Cheviet S, Coppola T, Haynes LP, Burgoyne RD, Regazzi R (Jan 2004). "The Rab-binding protein Noc2 is associated with insulin- ... Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. ...
"A novel rabconnectin-3-binding protein that directly binds a GDP/GTP exchange protein for Rab3A small G protein implicated in ... "The Rab-Binding Protein Noc2 Is Associated with Insulin-Containing Secretory Granules and Is Essential for Pancreatic β-Cell ... "Isolation and characterization of a GTPase activating protein specific for the Rab3 subfamily of small G proteins". The Journal ... Rab3 GTPase-activating protein catalytic subunit is an enzyme that in humans is encoded by the RAB3GAP1 gene. Members of the ...
... domain-containing proteins based on their Rab-binding activity". Genes to Cells. 11 (9): 1023-37. doi:10.1111/j.1365-2443.2006. ... GDP-bound) and active (GTP-bound) conformations. It also has the apolipophorin-III and tetratricopeptide repeat (TPR) domains. ... All of these proteins are in the guanosine nucleotide-binding protein family There are 63 orthologs of EVI5L that have been ... proteins, and functions as a GTPase-activating protein (GAP) with a broad specificity. Measurement of in vitro Rab-GAP activity ...
Zhu AX, Zhao Y, Flier JS (Dec 1994). "Molecular cloning of two small GTP-binding proteins from human skeletal muscle". ... Ras-related protein Rab-35 is a protein that in humans is encoded by the RAB35 gene. This GTPase participates in the traffic of ... "A Small Ras-like protein Ray/Rab1c modulates the p53-regulating activity of PRPK". Biochemical and Biophysical Research ...
FLCN acts as a GTPase-activating protein (GAP) toward Rag C/D GTPases, members of another Ras-related GTP-binding protein ... The DENN domain family of proteins are guanine nucleotide exchange factors (GEFs) for Rab proteins, members of the Ras ... October 2006). "Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved in AMPK ... Folliculin also known as FLCN, Birt-Hogg-Dubé syndrome protein or FLCN_HUMAN is a protein that in humans is associated with ...
Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein ... "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5". The EMBO Journal. 17 (7): ... "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5". The EMBO Journal. 17 (7): ... "Entrez Gene: RABEP1 rabaptin, RAB GTPase binding effector protein 1". Mattera R, Arighi CN, Lodge R, Zerial M, Bonifacino JS ( ...
... mG-proteins). As distinct from animal cells comprising 40 subfamilies of Rab proteins, which are the key... ... Rab proteins represent the largest family of monomeric GTP-binding proteins ( ... indicating specific for plants functions of Rab proteins.. Key words. plants monomeric GTP-binding proteins Rab proteins ... In plants, Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). As distinct from animal ...
The presence of members of this protein family was examined in Caco-2 cells by a PCR-based strategy. Twenty-five different ... Rab proteins belong to a subfamily of small GTP-binding protein genes of the Ras superfamily and play an important role in ... Rab proteins belong to a subfamily of small GTP-binding protein genes of the Ras superfamily and play an important role in ... Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in polarised ...
Rab proteins are the regulators of vesicles trafficking and plant responses to stresses, Russian Journal of Plant Physiology" ... In plants, Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). As distinct from animal ... Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). As distinct from animal cells ... Superfamily of plant monomeric GTP-binding proteins: 2. Rab proteins are the regulators of... Moshkov, I.; Novikova, G. 2011-01 ...
Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in polarised ... Role of GTP-binding proteins in the apical pathway.. X-chromosomale mentale retardatie (XMR): klinisch, cytogenetisch en ... Isolation and characterization of proteins involved in the routing of transport vesicles in polarized cells. ...
rab GTP-Binding Proteins/chemistry. *rab GTP-Binding Proteins/metabolism*. Substance. *rab GTP-Binding Proteins ... The full complement of yeast Ypt/Rab-GTPases and their involvement in exo- and endocytic trafficking.. Götte M1, Lazar T, Yoo ...
rab GTP-Binding Proteins/metabolism*. Substance. *rab GTP-Binding Proteins. LinkOut - more resources. Full Text Sources. * ... Crosstalk between multiple Rab GTPases through shared effectors, or through effectors that recruit selective Rab activators, ... Rab GTPases as coordinators of vesicle traffic.. Stenmark H1.. Author information. 1. Department of Biochemistry, Institute for ... Central in ensuring that cargoes are delivered to their correct destinations are the Rab GTPases, a large family of small ...
... acting as an effector of small GTPases including RAB-10 (Rab10). Here we present evidence that EHBP-1 associates with tubular ... Protein Binding / physiology * Protein Transport / physiology * Vesicular Transport Proteins / metabolism* * rab GTP-Binding ... domain binds to actin microfilaments in a reaction that is stimulated by RAB-10(GTP). Loss of any aspect of this RAB-10/EHBP-1 ... We propose a mechanism whereby RAB-10 promotes the ability of endosome-bound EHBP-1 to also bind to the actin cytoskeleton, ...
... which moves proteins and other molecules within cells in sac-like structures called vesicles. Learn about this gene and related ... The RAB23 gene provides instructions for making a protein that is involved in a process called vesicle trafficking, ... RAB family small GTP binding protein RAB 23. *RAB23_HUMAN. *ras-related protein Rab-23 ... Through the transport of certain proteins, the Rab23 protein regulates a specific developmental pathway called the hedgehog ...
RAB, RAS-related GTP-binding protein. rad, radiation-absorbed dose. RAG, recombination-activating gene ...
rab GTP-Binding Proteins. *Genetic Predisposition. *bcl-2-Associated X Protein. *Wiskott-Aldrich Syndrome Protein Family ... The introduction of antiproliferative agents such as insulin growth factor-binding protein 3 hold promise of a potentially ... Protein-protein interaction (PPI) networks of OS and normal groups were constructed and re-weighted using the Pearson ... as well as an increase in the fission protein dynamin-related protein 1 (Drp1). The results of this study suggest mitochondrial ...
EC 2.7.10.1/Proto-Oncogene Proteins c-ret; EC 2.7.10.1/RET protein, human; EC 3.6.1.-/rab GTP-Binding Proteins; EC 3.6.1.-/ ... Proto-Oncogene Proteins c-ret / genetics, metabolism*. Rats. Rats, Sprague-Dawley. rab GTP-Binding Proteins / metabolism. ... van der Sluijs P,Hull M,Webster P,Male P,Goud B,Mellman I. The small GTP-binding protein rab4 controls an early sorting event ... Protein Isoforms / genetics, metabolism. Protein Processing, Post-Translational. Protein Transport. Proteolysis. ...
Characterization of two cDNAs encoding Rab small GTP-binding proteins (Accession Nos. AF165095 and AF 165096) from cotton ( ... Characterization of a cDNA Encoding a Rac-like GTP-Binding Protein (Accession No. AF165925) from Cotton (Gossypium hirsutum L ... Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis. Planta, 218: 525-535. 2004. ... Cotton fiber germin-like protein. I. Molecular cloning and gene expression. Planta, 218: 516-524. 2004. ...
Protein Coding), RAB38, Member RAS Oncogene Family, including: function, proteins, disorders, pathways, orthologs, and ... Rab-Related GTP-Binding Protein 3 * Ras-Related Protein Rab-38 3 ... RAB GEFs exchange GTP for GDP on RABs. RAB GEFs exchange GTP ... Protein Symbol:. P57729-RAB38_HUMAN. Recommended name:. Ras-related protein Rab-38. Protein Accession:. P57729. Secondary ... Serological cloning of a melanocyte rab guanosine 5-triphosphate-binding protein and a chromosome condensation protein from a ...
0 (Membrane Proteins); 0 (Plant Proteins); 0 (SNARE Proteins); EC 3.6.5.2 (rab GTP-Binding Proteins). ... 0 (Flavoproteins); 0 (Photosystem I Protein Complex); 0 (Photosystem II Protein Complex); 0 (Plant Proteins); 1406-65-1 ( ... RAB GTPases and SNARE proteins are evolutionarily conserved key machineries of membrane trafficking mechanisms, which regulate ... Similar changes in subcellular localization were also observed for other fluorescently tagged SNARE proteins and a RAB GTPase, ...
ras GTPase-Activating Proteins. Recombinant Fusion Proteins. GTP-Binding Protein alpha Subunits. Endosomes. rab GTP-Binding ... Nerve Tissue Proteins. Protein Engineering. Sphingomyelins. Mutagenesis, Site-Directed. Transferases (Other Substituted ... Saccharomyces cerevisiae Proteins. Vacuoles. Vesicular Transport Proteins. Guanine Nucleotide Exchange Factors. ... rab GTP-Binding Proteins. 4. 2010. 2014. June 2012. Saccharomyces cerevisiae. 4. 2010. 2014. June 2012. ...
Protein Coding), RAB23, Member RAS Oncogene Family, including: function, proteins, disorders, pathways, orthologs, and ... RAB Family Small GTP Binding Protein RAB 23 3 * Ras-Related Protein Rab-23 3 ... Protein Symbol:. Q9ULC3-RAB23_HUMAN. Recommended name:. Ras-related protein Rab-23. Protein Accession:. Q9ULC3. Secondary ... Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set ...
... rab GTP-Binding Proteins, EC 3.6.1.-; Saccharomyces cerevisiae Proteins; SEC4 protein, S cerevisiae, EC 3.6.1.-. ... rab GTP-Binding Proteins · Saccharomyces cerevisiae · Saccharomyces cerevisiae Proteins · Sequence Alignment · Sequence ... Biology Pharmacology · Analytical research · Biomedical research · DNA · Fatty acid binding protein · Lipid · Liver protein · ... Carrier Proteins · Chromatography, High Pressure Liquid · Chromatography, Liquid · Fatty Acid-Binding Proteins · Genome · ...
Pereira-Leal, J. B. and Seabra, M. C. (2001). Evolution of the Rab family of small GTP-binding proteins. J. Mol. Biol. 313,889 ... 6E). We also reduced Rab11 activity via expression of a dominant-negative Rab11, Rab11N124I, a GTP-binding mutant (Duman et al ... Satoh, A. K. (1998). Rab proteins involved in the rhodopsin transport in Drosophila. In Department of Biology, pp. 124. Osaka: ... Zerial, M. and McBride, H. (2001). Rab proteins as membrane organizers. Nat. Rev. Mol. Cell Biol. 2, 107-117. ...
reconstitute endosome fusion using the Rab machinery along with SNARE proteins. This is the first report of the reconstitution ... Eukaryotic cells contain membrane-bound organelles that can undergo fusion with each other in a regulated manner. The widely ... efficiently and under physiological conditions and also highlights the functional relationship between Rab and SNARE proteins. ... fusion reactions with native SNAREs alone are inefficient and require high concentrations of proteins. In this study, Ohya et ...
Proteins isolated from cell lysates ranked by S score for interaction with GST-Rab4 (detergent-free, li ... GTP-Binding Proteins/metabolism*. *rab GTP-Binding Proteins/metabolism*. Minor. *Animals. *Protein Transport ... we found numerous Rab-specific interactions with known components of membrane traffic as well as with diverse proteins not ... we found numerous Rab-specific interactions with known components of membrane traffic as well as with diverse proteins not ...
A) Western blot analysis of Rab27B protein expression in 8 representative paired BCs (T) and NATs (N). Equal lo ... rab GTP-Binding Proteins/metabolism*. Minor. *Base Sequence. *Blotting, Western. *Cohort Studies ... A) Western blot analysis of Rab27B protein expression in 8 representative paired BCs (T) and NATs (N). Equal loading of protein ... A) Western blot analysis of Rab27B protein expression in 8 representative paired BCs (T) and NATs (N). Equal loading of protein ...
RAB6B, member RAS oncogene family, ras-related protein Rab-6B, small GTPase RAB6B, small GTP-binding protein. ...
J:24144 Chavrier P, et al., The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning ... protein coding gene. Chr11:100715003-100738215 (-). 129S1/SvImJ MGP_129S1SvImJ_G0019167. protein coding gene. Chr11:103480507- ... protein coding gene. Chr11:103388575-103414696 (-). CBA/J MGP_CBAJ_G0018887. protein coding gene. Chr11:111148158-111176966 (-) ... protein coding gene. Chr11:102236676-102268148 (-). BALB/cJ MGP_BALBcJ_G0019109. protein coding gene. Chr11:99652891-99676535 ...
Cheng, H., R. Sugiura, W. Wu, M. Fujita, Y. Lu et al., 2002 Role of the Rab GTP-binding protein Ypt3 in the fission yeast ... cycling between an active GTP-bound state and an inactive GDP-bound state. Thus, transport vesicles bear Rabs with bound GTP; ... In the cytosol, GDI binds Rab in the GDP-bound form. The heterodimeric complex of GDI and Rab-GDP serves as a cytosolic ... In addition to Rab binding, domain II, which consists of part of SCR2 and SCR3A, is required for both Rab extraction and Rab ...
Of particular interest is the dependence of these processes on multiple members of the Rab family of small GTP binding proteins ... Rab33b is a protein that localises to the Golgi apparatus and has suggested functions in both membrane trafficking and ... cells in order to maintain adequate energy levels and cellular homeostasis through the delivery of long-lived proteins and ... Of particular interest is the dependence of these processes on multiple members of the Rab family of small GTP binding proteins ...
Pereira-Leal, J. B. and Seabra, M. C. (2001). Evolution of the Rab family of small GTP-binding proteins. J. Mol. Biol. 313, 889 ... Bolte, S., Schiene, K. and Dietz, K. (2000). Characterization of a small GTP binding protein of the rab 5 family in ... Alternatively, the GDP-bound form of the Rab protein may interact specifically with a membrane-bound partner after release from ... This indicates that the protein is recruited to this organelle from the cytosol. The GDP-bound form of Rab protein is mainly ...
Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May also ... Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May also ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. Q8TBN0. K7CV42. Pan troglodytes (Chimpanzee). 382 ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ...
Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the ... Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the ... Protein-protein interaction databases. Protein interaction database and analysis system. More...IntActi. Q62739. 4 interactors. ... Protein-protein interaction databases. Protein interaction database and analysis system. More...IntActi. Q62739. 4 interactors. ...
... and ASTRAL compendium for protein structure and sequence analysis ... Keywords: Rab GTPase, SIGNALING PROTEIN, Cell membrane, GTP- ... Description: Crystal Structure of Rab28 GTPase in the Active (GppNHp-bound) Form. Class: signaling protein. ... Compound: Ras-related protein Rab-28. Species: Homo sapiens [TaxId:9606]. Gene: RAB28. Database cross-references and ... binding, Lipoprotein, Membrane, Nucleotide-binding, Prenylation. Deposited on 2008-08-13, released 2008-08-26. The last ...
Importantly, we used shRNA to knock-down Rab9 and VARP in macrophages and showed that these proteins are dispensable for Rab32 ... Importantly, we used shRNA to knock-down Rab9 and VARP in macrophages and showed that these proteins are dispensable for Rab32 ... Additionally, VPS9-ankyrin-repeat-protein (VARP) has been shown to regulate melanocytic enzyme trafficking into the melanosomes ... Additionally, VPS9-ankyrin-repeat-protein (VARP) has been shown to regulate melanocytic enzyme trafficking into the melanosomes ...
  • The full complement of yeast Ypt/Rab-GTPases and their involvement in exo- and endocytic trafficking. (nih.gov)
  • Rab GTPases as coordinators of vesicle traffic. (nih.gov)
  • Central in ensuring that cargoes are delivered to their correct destinations are the Rab GTPases, a large family of small GTPases that control membrane identity and vesicle budding, uncoating, motility and fusion through the recruitment of effector proteins, such as sorting adaptors, tethering factors, kinases, phosphatases and motors. (nih.gov)
  • Crosstalk between multiple Rab GTPases through shared effectors, or through effectors that recruit selective Rab activators, ensures the spatiotemporal regulation of vesicle traffic. (nih.gov)
  • EHBP-1 (Ehbp1) is a conserved regulator of endocytic recycling, acting as an effector of small GTPases including RAB-10 (Rab10). (nih.gov)
  • The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. (genecards.org)
  • DNA fragments containing genetic information for five secretion-related small GTPases of Aspergillus niger (srgA-E) were isolated and identified as members of different Rab/Ypt subfamilies. (tudelft.nl)
  • Toward a comprehensive map of the effectors of rab GTPases. (nih.gov)
  • Specificity of vesicle targeting is rendered in part by associated Rab GTPases. (biologists.org)
  • Rab GTPases are universal key regulators of intracellular secretory trafficking events. (biologists.org)
  • The communication between these organelles is mediated by small GTPases of the Rab subfamily ( Stenmark and Olkkonen, 2001 ). (biologists.org)
  • Rab GTPases are soluble proteins of ∼23 kDa and contain conserved amino acid sequences, which are necessary for nucleotide binding and GTP hydrolysis ( Olkkonen and Stenmark, 1997 ). (biologists.org)
  • Rab GTPases, such as Rab5, are found both on membranes and in the cytosol. (nature.com)
  • The Rab family represents the biggest member of Ras superfamily of small guanosine triphosphatases (GTPases) ( Zhen and Stenmark, 2015 ). (frontiersin.org)
  • Small Rab GTPases are highly conserved proteins containing more than 60 members in mammals ( Stenmark, 2009 ). (frontiersin.org)
  • Rab GTPases localize to a distinct organelle membranes or subcellular compartment and play a crucial role in orchestrating intracellular vesicle trafficking through tethering, docking, fusion and membrane exchange ( Hutagalung and Novick, 2011 ). (frontiersin.org)
  • Like other small GTPases, Rab proteins shuttle between their GTP-bound active state to GDP-bound inactive state. (frontiersin.org)
  • RAB proteins are members of the RAS superfamily of small GTPases, with which they share sequence and structural homology. (springer.com)
  • Subfamilies of the RAS GTPases include the RAB, RHO, ARF, RAP, and RAN proteins of which RABs are the largest group (Takai et al. (springer.com)
  • Review series: Rab GTPases and membrane identity: causal or inconsequential? (springer.com)
  • Gillingham AK, Sinka R, Torres IL, Lilley KS, Munro S. Toward a comprehensive map of the effectors of rab GTPases. (springer.com)
  • Rab GTPases containing a CAAX motif are processed post-geranylgeranylation by proteolysis and methylation. (springer.com)
  • To identify secretory membrane compartments involved this process, we took advantage of the characteristic that Rab GTPases are specifically recruited to different organelles in eukaryotic cells. (plantcell.org)
  • Rab GTPases are small GTP binding proteins that cycle between an inactive GDP-bound state and an active GTP-bound state. (plantcell.org)
  • In their membrane-associated, GTP-bound state, Rab GTPases undergo conformational changes allowing subsequent recruitment of additional cytosolic proteins to that subcellular compartment. (plantcell.org)
  • In eukaryotic organisms, Rab GTPases regulate membrane trafficking events associated with distinct compartments (reviewed in Zerial and McBride, 2001 ). (plantcell.org)
  • Phagosomes offer kinetically and morphologically tractable organelles to dissect the control of phagolysosome biogenesis by Rab GTPases. (rupress.org)
  • Rab GTPases and their effectors coordinate vesicle transport. (teachspatial.org)
  • Determinants of the broad recognition of exocytic Rab GTPases by Mss4" by Zhongyuan Zhu, Anna M. Delprato et al. (umassmed.edu)
  • Rab GTPases function as essential regulators of vesicle transport between subcellular compartments of eukaryotic cells. (umassmed.edu)
  • Mss4, an evolutionarily conserved Rab accessory factor, facilitates nucleotide release and binds tightly to the nucleotide-free form of exocytic but not endocytic Rab GTPases. (umassmed.edu)
  • A structure-based mutational analysis of residues that are conserved only in exocytic Rab GTPases reveals three residues that are critical determinants of the broad specificity recognition of exocytic Rab GTPases by Mss4. (umassmed.edu)
  • The complementarity of the corresponding interaction surfaces suggests a hypothetical structural model for the complex between Mss4 and Rab GTPases. (umassmed.edu)
  • After nucleotide exchange to the active (GTP-bound) conformation, Rab GTPases interact with functionally diverse effectors including lipid kinases, motor proteins and tethering complexes. (rcsb.org)
  • How effectors distinguish between homologous Rab GTPases represents an unresolved problem with respect to the specificity of vesicular trafficking. (rcsb.org)
  • The results demonstrate that even the structurally similar effector domains in rabenosyn-5 can achieve highly selective recognition of distinct subsets of Rab GTPases exclusively through interactions with the switch and interswitch regions. (rcsb.org)
  • The observed specificity is determined at a family-wide level by structural diversity in the active conformation, which governs the spatial disposition of critical conserved recognition determinants, and by a small number of both positive and negative sequence determinants that allow further discrimination between Rab GTPases with similar switch conformations. (rcsb.org)
  • Rab GTPases are implicated in vesicle trafficking. (embl.de)
  • Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? (embl.de)
  • GTPase activating proteins (GAPs) from pathogenic bacteria and eukaryotic host organisms deactivate Rab GTPases by supplying catalytic arginine and glutamine fingers in trans and utilizing the cis-glutamine in the DXXGQ motif of the GTPase for binding rather than catalysis. (umassmed.edu)
  • Rab proteins constitute the largest family of small GTPases with almost 70 members in humans. (embopress.org)
  • Rab GTPases are essential mediators of membrane traffic, playing a role in the majority of cellular traffic of vesicles and organelles. (bspp.org.uk)
  • Rab GTPases are conventionally considered active when GTP-bound and inactive in their GDP-bound form. (bspp.org.uk)
  • In its GDP bound form, newly synthesised Rab GTPases undergo geranylgeranylation which directs membrane attachment. (bspp.org.uk)
  • Rab GTPase activating proteins (GAPs) catalyze hydrolysis of the GTP bound to active Rab GTPases to inactivate them. (bspp.org.uk)
  • Rab GTPases constitute the largest family of small GTPases. (frontiersin.org)
  • Rab GTPases cycle between active GTP-bound and inactive GDP-bound states by GEF, GAP, and other GTPase-activating proteins ( Stenmark, 2009 ). (frontiersin.org)
  • Insights into the developmental regulation of CPs and Rab small GTPases during encystation are also discussed as examples of transcriptomic approaches to understand the biology and pathogenesis of this group of enteric protozoa. (asmscience.org)
  • This review summarizes and discusses the evidence implicating Ras, Rho, Arf and Rab-family GTPases in the signalling pathways driving particle recognition and uptake. (stir.ac.uk)
  • Our research focuses on vesicular trafficking events controlled by the monomeric Rab GTPases. (ucc.ie)
  • Motor proteins that interact with Rab GTPases of the endocytic recycling pathway. (ucc.ie)
  • Rab11 and Rab14 are two related Rab GTPases that are believed to function in endosomal recycling and Golgi/endosome transport processes. (ucc.ie)
  • The family of 60 Rab GTPases constitutes the largest and most diverse group of Ras-like small G proteins ( 68 ). (asm.org)
  • Rab GTPases are anchored to the cytoplasmic face of intracellular compartments via a geranyl-geranyl group in the lipid bilayer, and such interactions are important for their proper functioning ( 49 ). (asm.org)
  • Small GTPases of the rab subfamily are involved in regulation of intracellular membrane transport events. (semanticscholar.org)
  • therefore, it has been suggested that rab GTPases may be involved in the tethering of membranes by recruiting effectors to vesicle and target membranes. (embopress.org)
  • OC differentiation, survival, and activity are regulated by numerous small GTPases, including those of the Rab family, which are involved in plasma membrane delivery and lysosomal and autophagic degradation pathways. (hindawi.com)
  • Bone resorption has been associated with dynamic membrane processes that are regulated by small GTPases of the Rab family and by their regulators and effectors. (hindawi.com)
  • Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. (genecards.org)
  • hence, their role remains unclear.For many Rabs, this revealed specific interactions with Drosophila orthologs of known effectors.In addition, we found numerous Rab-specific interactions with known components of membrane traffic as well as with diverse proteins not previously linked to organelles or having no known function. (nih.gov)
  • In addition to CG31064, there were several other proteins that were specific to Rab4 that have not previously been reported to be effectors. (nih.gov)
  • Together with the cross-talk between different Rab proteins and their effectors, this may provide an integrated system for the general coordination of endocytic pathways to maintain organelle homeostasis. (biologists.org)
  • the recruitment of Rab5 effectors is stabilized by SNARE proteins and SNARE-protein accessory factors. (nature.com)
  • Multiple downstream effectors of small G proteins, some of them being protein kinases, have been identified. (ahajournals.org)
  • Recent findings, however, indicate that their function in vesicle trafficking can go beyond this role, and a number of proteins, unrelated to each other, have been identified as putative Rab effectors. (embl.de)
  • RAB effectors include rabphilin, RIMs, RAB GDP dissociation inhibitor ( RABGDI ), RAB GTPase activating protein ( RAB3GAP ), RAB GDP/GTP exchange protein ( RAB3GEP ) and guanine nucleotide exchange factors (GEFs), among others. (biomedcentral.com)
  • We, and others, have identified a group of proteins that interact with Rab11 and function as Rab11 effectors, known as the Rab11-FIPs (family of interacting proteins). (ucc.ie)
  • Interestingly, a broad-host pathogen Salmonella enterica serovar Typhimurium ( S . Typhimurium) has evolved two effector proteins, GtgE, and SopD2, to counteract the Rab32/BLOC-3 dependent antimicrobial pathway (BRAM pathway). (frontiersin.org)
  • We propose that endosomes are organized as a mosaic of different Rab domains created through the recruitment of specific effector proteins, which cooperatively act to generate a restricted environment on the membrane. (rupress.org)
  • In the GTP-bound state Rab proteins recruit specific effector proteins to the membrane. (rupress.org)
  • The Rab11-FIPs - a family of related effector proteins that were originally identified by their ability to interact with Rab11. (ucc.ie)
  • Several approaches have been taken to trace out rab effector proteins acting downstream of or alongside the rab proteins. (embopress.org)
  • Subsequent autophosphorylation of intracellular c-fms domains leads to the recruitment and docking of PTB- (phosphotyrosine-binding) and SH2- (Src homology region 2) domain-containing effector proteins and activation of the signaling pathways that promote OC proliferation, survival, and motility. (hindawi.com)
  • Rab33b is a protein that localises to the Golgi apparatus and has suggested functions in both membrane trafficking and autophagic processes. (mdpi.com)
  • These results establish a role for Rab11 in the post-Golgi transport of rhodopsin and of other proteins to the rhabdomeric membranes of photoreceptors, and in analogous transport processes in other cells. (biologists.org)
  • Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport. (springer.com)
  • We identified EAT-17 as a novel RabGAP that interacts with RAB-6.2 , a protein that presumably regulates vesicle trafficking between Golgi, the endoplasmic reticulum, and plasma membrane to form a functional grinder. (genetics.org)
  • Whereas a small fraction of RabA4b cofractionated with Golgi membrane marker proteins, the majority of this protein labeled a unique membrane compartment that did not cofractionate with the previously characterized trans -Golgi network syntaxin proteins SYP41 and SYP51. (plantcell.org)
  • Fragmentation of the mammalian Golgi apparatus during mitosis requires the phosphorylation of a specific subset of Golgi-associated proteins. (rupress.org)
  • Golgin-84 binds to active rab1 but not cis-Golgi matrix proteins. (rupress.org)
  • These mini-stacks are able to carry out protein transport, though with reduced efficiency compared with a normal Golgi apparatus. (rupress.org)
  • Proteins that enter the cis-Golgi network are either recycled back to the ER or transported onwards through the Golgi stack where they can be modified by the sequential action of enzymes present in each of the individual cisternae. (rupress.org)
  • Upon arrival at the trans-Golgi network, proteins are packaged into carriers for delivery to the plasma membrane or endosomes. (rupress.org)
  • Golgin-45 is required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. (ebi.ac.uk)
  • It interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins [ PMID: 11739401 ]. (ebi.ac.uk)
  • COPI vesicles transport proteins retrograde between golgi compartments, and from cis-golgi to ER clathrin vesicles transport proteins from plasma membrane and trans-golgi to endosomes (ex. (brainscape.com)
  • GLUT4 protein is synthesized in the endoplasmic reticulum and travels to the trans-Golgi network (TGN), where GLUT4 is then inserted into highly specialized vesicles. (diabetesincontrol.com)
  • Transport vesicles can move molecules between locations inside the cell, e.g., proteins from the rough endoplasmic reticulum to the Golgi apparatus . (wikipedia.org)
  • Most of these proteins mature in the Golgi apparatus before going to their final destination which may be to lysosomes , peroxisomes , or outside of the cell. (wikipedia.org)
  • We show that pre-Golgi and Golgi compartments, as well as secretory vesicles, are sites of ethanol-induced retention of nascent soluble and transmembrane secretory proteins. (elsevier.com)
  • The effects of ethanol exposure on tab proteins and Golgi function are discussed. (elsevier.com)
  • The encoded protein is found in the Golgi body and interacts with RPGRIP1. (mybiosource.com)
  • To detect specific partners of the small Golgi‐localized GTPase rab1b we generated rab1b mutants and used them as bait proteins in yeast two‐hybrid screens. (embopress.org)
  • Phenotypic analysis of gene deletion strains revealed that loss of both VPS21 and YPT52 causes synthetic defects in endocytic trafficking to the vacuole, as well as delivery of the virulence-associated vacuolar membrane protein Mlt1p from the Golgi compartment. (elsevier.com)
  • The RAB23 gene provides instructions for making a protein that is involved in a process called vesicle trafficking, which moves proteins and other molecules within cells in sac-like structures called vesicles. (medlineplus.gov)
  • Other RAB23 gene mutations that cause Carpenter syndrome reduce or eliminate function of the Rab23 protein. (medlineplus.gov)
  • Ras-related protein Rab-15 is a protein that in humans is encoded by the RAB15 gene. (wikipedia.org)
  • RAB38 (RAB38, Member RAS Oncogene Family) is a Protein Coding gene. (genecards.org)
  • Gene Ontology (GO) annotations related to this gene include GTP binding . (genecards.org)
  • In the same screen, we isolated gdi1-i11 , a mutant allele of the essential gdi1 + gene encoding Rab GDP-dissociation inhibitor. (genetics.org)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • In general, the Ras family mainly regulates gene expression, the Rho family regulates both cytoskeletal reorganization and gene expression, the Rab and Sar1/Arf families regulate intracellular vesicle trafficking, and the Ran family regulates nucleocytoplasmic transport and microtubule organization during the cell cycle. (ahajournals.org)
  • The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. (abnova.com)
  • 1988 ). Efforts to clone other members of the RAB gene family quickly established that this family had undergone significant expansion in higher eukaryotes. (springer.com)
  • This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. (origene.com)
  • We have characterized (1) correct assembly and folding of tyrosinase-gene family proteins with the help of calnexin, (2) target signals of tyrosinase-gene family proteins from TGN to melanosomes, (3) functional interactionbetween tyrosinase and lysosome-associated membrane protein in melanosome biogenesis, (4) mechanism of abnormal melanin synthesis in vitiligo melanocytes and (5) chemical modification of melanin precursor and development of depigmentation. (nii.ac.jp)
  • Publications] J0116imbow K et al: 'Biological role of tyrosinase related protein and its biosynthesis and transport from TGN to stage I melanosome late endosome,through gene transfection study. (nii.ac.jp)
  • Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. (abnova.com)
  • Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. (abnova.com)
  • This gene encodes a member of the Rab40 subfamily of Rab small GTP-binding proteins that contains a C-terminal suppressors of cytokine signaling box. (nih.gov)
  • A positional cloning approach indicates that the mutation in aura is associated with a truncation of Mid1 Interacting Protein 1L (Mid1ip1L), previously identified as an interactor of the X-linked Opitz G/BBB syndrome gene Mid1. (zfin.org)
  • The ras-related ypt protein is an ubiquitous eukaryotic protein: isolation and sequence analysis of mouse cDNA clones highly homologous to the yeast YPT1 gene. (embl.de)
  • The YPT1 gene of the yeast Saccharomyces cerevisiae codes for a guanine nucleotide-binding protein which is essential for cell viability. (embl.de)
  • The cDNAs were derived from a gene, designated ypt1, which codes for a protein of 205 amino acids with 71% homology to the yeast YPT1 gene product. (embl.de)
  • A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product. (embl.de)
  • The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements.GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. (acris-antibodies.com)
  • Evolutionary rate analysis revealed that presynaptic proteins evolve slowly, although some members of large gene families exhibit accelerated evolutionary rates relative to other family members. (biomedcentral.com)
  • The majority of ribosomal protein genes are well conserved, and only the gene for the large subunit protein L41 has not been identified. (asmscience.org)
  • This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. (mybiosource.com)
  • has shown that, in tomato ( Lycopersicon esculentum ) fruit, the transcription of a gene, which showed high homology to a monomeric G-protein from pea, is rapidly but transiently up-regulated by ethylene. (plantphysiol.org)
  • The Gdi1 G267D protein failed to extract Rabs from membrane and Rabs were depleted from the cytosolic fraction in the gdi1-i11 mutant cells. (genetics.org)
  • In mammals, Rabs define a family of almost 70 proteins that play critical roles in the trafficking of vesicles that mediate transport between compartments of the exocytic and endocytic pathways ( P feffer 2001 , 2005 ). (genetics.org)
  • Like Ras, Rabs act as molecular switches, cycling between an active GTP-bound state and an inactive GDP-bound state. (genetics.org)
  • concomitant with or after membrane fusion, Rabs are converted into their GDP-bound states. (genetics.org)
  • GDI binds and releases Rabs in the presence of GDP but not GTP, which is thought to be a key biochemical function of GDI in retrieving Rabs from target membranes. (genetics.org)
  • Thus, GDI plays a key role in the Rab recycling by retrieving prenylated Rabs from their fusion targets in membranes, maintaining Rabs as a soluble cytosolic complex and delivering Rabs to specific membrane-bound compartments for vesicle formation. (genetics.org)
  • RAB GEFs exchange GTP for GDP on RABs. (uniprot.org)
  • B) Anti-GFP immunoblot of affinity chromatography of lysates from S2 cells expressing GFP-CG31064 (FYVE domain form) or CG7371-GFP using GDP- and GTP-locked Rabs. (nih.gov)
  • Linker proteins such as PRA1 bind to both Rabs and SNAREs. (nature.com)
  • Considerable evidence indicates that phosphorylation of TBC1D4 suppresses its GAP activity, thereby promoting elevation of the GTP-bound form of selected Rabs and GLUT4 translocation ( 7 - 9 ) ( Fig. 1 ). (pnas.org)
  • Although multiple small GTPase Rab proteins are required for the process, few regulators of Rabs are known. (genetics.org)
  • RNA interference against 25 confirmed and/or predicted RABs in C. elegans shows that RNAi against rab-6.2 produces a phenotype identical to eat-17 . (genetics.org)
  • In those cases, removing the function of the RabGAPs produced phenotypes almost identical to those of removing the relevant Rabs, showing the fundamental roles of RabGAPs in modulating Rab functions. (genetics.org)
  • GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. (acris-antibodies.com)
  • Other proteins that modulate the activity of endocytic Rabs e.g. (ucc.ie)
  • Fusion specificity is controlled by several protein factors, including Rabs, SNAP receptors, and tethering proteins ( 47 ). (asm.org)
  • Rab proteins constitute a large family of monomeric GTP-binding proteins that regulate intracellular vesicle transport. (springermedizin.de)
  • The diversity of Rab proteins in vesicle transport. (embl.de)
  • RAB proteins, at least RAB3 , RAB5 and RAB11 family members, form a large set of GTP-binding proteins that regulate vesicle transport, docking, and late steps in exocytosis. (biomedcentral.com)
  • 1994 ). Rab escort protein-1 is a multifunctional protein that accompanies newly prenylated rab proteins to their target membranes. (biologists.org)
  • 1997 ). Mitotic phosphorylation of rab4 prevents binding to a specific receptor on endosome membranes. (biologists.org)
  • 1995 ). Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes. (biologists.org)
  • Specifically, Rab proteins are involved in the recruitment of effector molecules on cellular membranes, which in turn regulate membrane docking and fusion ( Stenmark and Olkkonen, 2001 ). (biologists.org)
  • This delivery and removal is mediated by the membrane-bound organelles of eukaryotic cells, which communicate with each other by budding off vesicles and other transport packages, which travel to, and fuse with, target membranes. (nature.com)
  • The membrane-bound SNARE proteins link the two membranes destined for fusion. (nature.com)
  • Lipidated Rho and Rab GTP-binding proteins are transported between membranes in complex with solubilizing factors called 'guanine nucleotide dissociation inhibitors' (GDIs). (rcsb.org)
  • Immunoblotting of urinary membrane fractions has revealed that, in addition to AQP2, the kidneys excrete membranes containing apical plasma-membrane transporter proteins from each renal tubule segment ( 5 ), suggesting that analysis of urinary membrane fractions could provide noninvasive information about the pathophysiological state of the entire renal tubule. (pnas.org)
  • In eukaryotic cells, proteins and membranes are sorted and delivered to specific cellular destinations through a series of interconnected membrane trafficking pathways. (plantcell.org)
  • The Rab family of small GTP-binding proteins has long been implicated in the docking and fusion of transport vesicles with their target membranes. (embl.de)
  • The Rab GTPase family of proteins are mediators of membrane trafficking, conferring identity to the cell membranes. (edu.au)
  • Thus, GLUT proteins are intimately embedded in membranes, and the most highly conserved regions are the putative membrane-spanning domains that serve a common function, the creation of a pore for facilitative diffusion of monosaccharides. (diabetesincontrol.com)
  • According to this view, SNARE molecules are involved in docking between donor and acceptor membranes, while another set of proteins participates in subsequent stages of the fusion process. (embopress.org)
  • We have demonstrated that association between FIP3 and DLIC-1 at the cell periphery precedes minus-end-directed microtubule-based transport, that FIP3 recruits DLIC-1 onto membranes, and that knockdown of DLIC-1 inhibits pericentrosomal accumulation of key endosomal-recycling compartment (ERC) proteins. (ucc.ie)
  • Isolation and characterization of proteins involved in the routing of transport vesicles in polarized cells. (ru.nl)
  • Sec12pdependent membrane binding of the small GTP-binding protein Sar1 p promotes formation of transport vesicles from the ER. (springer.com)
  • Rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles. (springer.com)
  • A GTPbinding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. (springer.com)
  • Proteomic analysis of urinary vesicles through nanospray liquid chromatography-tandem mass spectrometry identified numerous protein components of MVBs and of the endosomal pathway in general. (pnas.org)
  • We hypothesize that AQP2 and other apical plasma-membrane proteins are excreted through the process of exosome formation, i.e., delivery of the internal vesicles of multivesicular bodies (MVBs) to the urinary space by fusion of the outer membrane of MVBs with the apical plasma membrane of renal tubule epithelial cells. (pnas.org)
  • Third, the urinary vesicles should contain proteins typical of MVBs and of exosomes formed by other cell types ( 7 ). (pnas.org)
  • Rab family small GTP-binding proteins identified in AQP2-vesicles from inner medullary collecting duct. (teachspatial.org)
  • All Rab proteins listed were identified in the AQP2-containing vesicles except Rab3. (teachspatial.org)
  • These proteins travel within the cell inside of transport vesicles. (wikipedia.org)
  • Membrane proteins located on vesicles (v‐SNAREs) and on the target membrane (t‐SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. (embopress.org)
  • These multiprotein complexes are composed of proteins that are bound constitutively or transiently to the synaptic vesicles or plasma membrane. (biomedcentral.com)
  • The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane. (ucc.ie)
  • Where are proteins/cargo carried in vesicles? (cram.com)
  • The docking and fusion of vesicles is, however, a complex multistep reaction, and the precise point at which Rab proteins act in these sequential processes in unknown. (ucl.ac.uk)
  • In brain, the Rab protein Rab3A is specific to synaptic vesicles, whose exocytosis can be monitored with submillisecond resolution by following synaptic transmission. (ucl.ac.uk)
  • Among its related pathways are Cytoskeletal Signaling and Metabolism of proteins . (genecards.org)
  • Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Metabolism of proteins . (genecards.org)
  • This review will focus on key signaling intermediates of the IR and IGF-1R, the Insulin Receptor Substrate (IRS) proteins, with an emphasis on IRS-2, and discuss how these adaptor proteins play a pivotal role at the intersection of metabolism and cancer. (pubmedcentralcanada.ca)
  • Rab proteins belong to a subfamily of small GTP-binding protein genes of the Ras superfamily and play an important role in intracellular vesicular targeting. (nih.gov)
  • We have previously identified mutant alleles of genes encoding two Rab proteins, Ypt3 and Ryh1, through a genetic screen using the immunosuppressant drug FK506 in fission yeast. (genetics.org)
  • Full liquid chromatography-tandem MS analysis revealed 295 proteins, including multiple protein products of genes already known to be responsible for renal and systemic diseases, including autosomal dominant polycystic kidney disease, Gitelman syndrome, Bartter syndrome, autosomal recessive syndrome of osteopetrosis with renal tubular acidosis, and familial renal hypomagnesemia. (pnas.org)
  • Mutations in Rab‐coding genes and/or post‐translational modifications in their protein products disrupt the cellular vesicle trafficking network modulating tumorigenic potential, cellular migration and metastatic behavior. (edu.au)
  • A lot of heat-responsive genes and proteins including Hsps, Hsfs, antioxidant enzymes, various transcription factors and calmodulin have been identified and their functions were well elucidated [9] - [12] . (pubmedcentralcanada.ca)
  • Multiple glucose transporter genes have been identified [66,135] that encode a family of homologous proteins exhibiting different functional properties and marked differences in tissue-specific expression. (diabetesincontrol.com)
  • The kinetics of the steady-state levels of mRNA for individual Rab genes were categorized into cyst-specific, trophozoite-specific, or constitutive genes. (asmscience.org)
  • Rab4 Interacts with a Second Form of the GARP Complex(A) Proteins isolated from cell lysates ranked by S score for interaction with GST-Rab4 (detergent-free, lighter gray). (nih.gov)
  • AT 1 interacts with multiple heterotrimetric G proteins, including G q/11 , G i , G 12 , and G 13 , and produces second messengers, such as inositol triphosphate, diacylglycerol, and reactive oxygen species (ROS). (ahajournals.org)
  • ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2. (bioportfolio.com)
  • GTP‐bound Rab7b interacts with Atg4B. (embopress.org)
  • We have shown that the Rab11 GTPase effector protein Rab11-FIP3 directly interacts with the dynein light intermediate chain 1 (DLIC-1) subunit of the cytoplasmic dynein 1 motor protein complex. (ucc.ie)
  • Here we report for the first time that the rab1b protein interacts specificially with GM130 in a GTP‐dependent manner and therefore needs the hypervariable regions of the N‐ and C‐termini. (embopress.org)
  • Using the yeast two‐hybrid approach we surprisingly identified GM130 as a protein that interacts directly and in a GTP‐dependent manner with rab1b. (embopress.org)
  • Among binding domains of p62, the ubiquitin-associated (UBA) domain interacts with polyubiquitinated proteins and mediates their degradation via autophagic or proteasomal pathways [ 10 ], which are reportedly central to OC activities [ 11 , 12 ]. (hindawi.com)
  • 1991 ). Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2. (biologists.org)
  • Phosphorylation of two small GTP-binding proteins of the rab family by p34 cdc2. (springer.com)
  • Tre-2, BUB2, CDC16, 1 domain family member 4 (TBC1D4) is a 160-kDa AKT substrate with a GTPase-activating protein (GAP) domain and several AKT phosphorylation sites ( 6 , 7 ). (pnas.org)
  • Phosphorylation of proteins related to EGFR signaling was examined by western blot analysis. (frontiersin.org)
  • Implicit in the presence of a putative MAPKKK in the transduction sequence is that protein phosphorylation via a mitogen-activated protein kinase (MAPK) cascade(s) is involved in mediating responses to ethylene. (plantphysiol.org)
  • Both AMP and ADP binding to the γ subunit enhances T172 phosphorylation and suppresses dephosphorylation [ 11 - 13 ]. (koreamed.org)
  • Superfamily of plant monomeric GTP-binding proteins: 2. (springer.com)
  • In plants, Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). (springer.com)
  • Novikova, G. 2011-01-18 00:00:00 In plants, Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). (deepdyve.com)
  • Small GTP-binding proteins (G proteins) are monomeric G proteins with a low molecular weight of 20 to 40 kDa. (ahajournals.org)
  • It is demonstrated that, in etiolated pea ( Pisum sativum ) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. (plantphysiol.org)
  • For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transient down-regulation. (plantphysiol.org)
  • Immunoprecipitation studies indicate that some of the monomeric G-proteins affected may be of the Rab class. (plantphysiol.org)
  • Other candidates that have emerged as possible components of the ethylene signal transduction pathway are monomeric GTP-binding proteins (monomeric G-proteins). (plantphysiol.org)
  • it has been shown that ethylene at physiological concentrations activates monomeric G-proteins. (plantphysiol.org)
  • A genetically related subfamily of RAB GTP-BINDING PROTEINS involved in calcium-dependent EXOCYTOSIS. (definitions.net)
  • These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters. (thermofisher.com)
  • Functional impairments of Rab pathways are associated with diseases, such as immunodeficiencies, cancer and neurological disorders. (nih.gov)
  • This review summarizes current knowledge about Rab GTPase functions in the endocytic pathways and provides insight into the regulation of Rab GTPase activity and mechanisms of Rab protein function. (biologists.org)
  • Functional assays have identified some Rab proteins that operate on individual pathways, but Rab proteins in several pathways remain controversial or have not been identified. (biologists.org)
  • This paradigm will most likely be generally applicable to other Rab GTPase pathways. (biologists.org)
  • Investigations into these proteins have indicated roles in both the endocytic and vacuolar trafficking pathways. (biologists.org)
  • In this review, we describe detailed mechanisms of signal transduction pathways of Ang II involving small G proteins in VSMCs together with their functional significances in mediating vascular remodeling. (ahajournals.org)
  • Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. (origene.com)
  • To characterize membrane trafficking pathways involved in these processes, we have examined the function of a plant Rab GTPase, RabA4b, during polarized expansion in developing root hair cells. (plantcell.org)
  • In eukaryotic cells, protein transport through the secretory and endocytic pathways is mediated by vesicular intermediates. (embl.de)
  • Recently, Rab and Rab‐associated factors have been recognized as major regulators of the intracellular positioning and activity of signaling pathways regulating cell growth, survival and programmed cell death or apoptosis. (edu.au)
  • The mitogen-activated protein kinases (MAPKs) have been shown to regulate skeletal muscle function. (bireme.br)
  • About half of all biological processes involve membrane proteins, which must be delivered, and eventually removed, with great accuracy to regulate the constancy of structural identity. (nature.com)
  • In melanocytes, Rab9 has been shown to interact with HPS4 and RUTBC1, a Rab32 GTPase activating (GAP) protein, and regulate the Rab32-mediated melanosome biogenesis. (frontiersin.org)
  • Additionally, VPS9-ankyrin-repeat-protein (VARP) has been shown to regulate melanocytic enzyme trafficking into the melanosomes through interaction with Rab32. (frontiersin.org)
  • RAB21 belongs to the RAB family of small GTP-binding proteins that regulate intracellular vesicle targeting (Opdam et al. (bio-rad.com)
  • GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. (acris-antibodies.com)
  • A Phosphoproteomic Screen Identifies a Guanine Nucleotide Exchange Factor for Rab3A Protein as a Mitogen-activated Protein (MAP) Kinase Phosphatase-5-regulated MAP Kinase Target in Interleukin 6 (IL-6) Secretion and Myogenesis. (bireme.br)
  • Rab proteins alternate between GDP-bound and GTP-bound forms, a switch controlled by proteins called guanine-nucleotide exchange factors. (nature.com)
  • Prototypically, the growth factor receptor on activation recruits a Ras guanine nucleotide exchange factor (GEF), Sos, via adaptor proteins Shc and Grb2. (ahajournals.org)
  • Guanine quadruplexes can form in both DNA and RNA and influence many biological processes through various protein interactions. (bioportfolio.com)
  • Individual transport steps are regulated by Ras-like guanine nucleotide-binding proteins, termed Ypt in yeast or Rab in mammals. (embl.de)
  • Amino acid sequences typical for guanine nucleotide-binding proteins and characteristic for ypt proteins are perfectly conserved in the mouse ypt1 protein. (embl.de)
  • RABIF is a guanine-nucleotide-releasing protein is involved in vesicular transport. (prospecbio.com)
  • As distinct from animal cells comprising 40 subfamilies of Rab proteins, which are the key regulators of intracellular vesicular transport, numerous Rab proteins in Arabidopsis and other plant species could be grouped in only eight subfamilies on the basis of their functional properties. (springer.com)
  • Based on the conserved function of Rab6 in vesicular transport, we propose that EAT-17 regulates the turnover rate of RAB-6.2 activity in cargo trafficking for grinder formation. (genetics.org)
  • RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. (origene.com)
  • A variety of cell‐free systems that reconstitute distinct transport steps were utilized to identify many proteins implicated in intracellular vesicular transport. (embopress.org)
  • RET51 is internalized faster after ligand binding and undergoes recycling back to the plasma membrane. (biomedsearch.com)
  • During photoreceptor terminal differentiation, massive biosynthetic membrane traffic delivers rhodopsin and other phototransduction proteins to an apical plasma membrane subdomain to form photosensory organelles, invertebrate rhabdomeres and vertebrate outer segments. (biologists.org)
  • Here we use immunoelectron microscopy and nanospray liquid chromatography-tandem MS (LC-MS/MS) analysis of urinary membrane proteins to show that AQP2 and other apical plasma-membrane proteins are excreted through the process of exosome formation in agreement with the predictions above. (pnas.org)
  • LecRLKs are plasma membrane proteins believed to be involved in signal transduction. (deepdyve.com)
  • Binding of ligands to EGFR induces EGFR autophosphorylation at the plasma membrane and its internalization to the early endosome, where EGFR is sorted for recycling to the cell surface or degradation in the lysosome. (frontiersin.org)
  • and protein localization to plasma membrane. (jax.org)
  • abstract = "Chronic ethanol consumption induces hepatocellular retention of nascent proteins leading to hepatomegaly. (elsevier.com)
  • The RET9 and RET51 isoforms display unique levels of autophosphorylation and have differential interactions with adaptor proteins. (biomedsearch.com)
  • Autophagy (particularly macroautophagy) is a bulk degradation process used by eukaryotic cells in order to maintain adequate energy levels and cellular homeostasis through the delivery of long-lived proteins and organelles to the lysosome, resulting in their degradation. (mdpi.com)
  • This family also includes several higher eukaryotic proteins. (ebi.ac.uk)
  • A subfamily of small GTP-binding proteins, rab, has been shown to be involved in regulation of vesicular traffic in eukaryotic cells. (elsevier.com)
  • Rab proteins are master controllers of eukaryotic intracellular trafficking events of both the secretory and endocytic pathway. (ucc.ie)
  • Several protein families control intracellular transport processes in eukaryotic cells. (ucc.ie)
  • Small GTP- binding proteins (rab proteins) have recently been implicated in the regulation of vesicular trafficking in eukaryotic cells. (elsevier.com)
  • In eukaryotic cells, it is an essential process in the recycling or removal of membrane proteins and lipids, the transport of proteins and lipids into cells for degradation and the uptake of signal molecules ( 34 ). (asm.org)
  • Fukui K, Sasaki T, Imazumi K, Matsuura Y, Nakanishi H, Takai Y. Isolation and characterization of a GTPase activating protein specific for the Rab3 subfamily of small G proteins. (springer.com)
  • The presence of members of this protein family was examined in Caco-2 cells by a PCR-based strategy. (nih.gov)
  • Twenty-five different partial cDNA sequences were isolated, including 18 Rab protein family members. (nih.gov)
  • Of particular interest is the dependence of these processes on multiple members of the Rab family of small GTP binding proteins. (mdpi.com)
  • RAB27A is a member of the Small GTPase superfamily, Rab family. (biocompare.com)
  • In Arabidopsis there are 57 members of the Rab GTPase family. (biologists.org)
  • These include the SNARE family of membrane proteins and the Rab proteins, a subfamily of the Ras superfamily of GTPase enzymes. (nature.com)
  • Rab38 is a member of the Rab small G protein family and is mainly expressed in lung alveolar type II cells and melanocytes. (creative-biogene.com)
  • Tre-2, BUB2, CDC16, 1 domain family member 4 (TBC1D4) (AS160) is a Rab-GTPase activating protein implicated in insulin-stimulated glucose transporter 4 (GLUT4) translocation in adipocytes and myotubes. (pnas.org)
  • However, most of the members of the protein family even in plants have not been functionally well characterized. (deepdyve.com)
  • Using a structural proteomic approach, we have determined the specificity and structural basis underlying the interaction of the multivalent effector rabenosyn-5 with the Rab family. (rcsb.org)
  • Although the GTPase switch of Rab proteins is highly conserved, functional mechanisms may be highly diversified among members of the Rab family. (embl.de)
  • Small GTP-binding proteins of the RAB family, such as RAB31, play essential roles in vesicle and granule targeting (Bao et al. (genetex.com)
  • This protein family has been sub-classified into two groups - class I FIPs [FIP2, RCP (Rab coupling protein) and Rip11 (Rab11-interacting protein)] and class II FIPs (FIP3 and FIP4). (ucc.ie)
  • Class I Rab11-family interacting proteins are binding targets for the Rab14 GTPase. (ucc.ie)
  • Rab24 is a member of the Rab GTPase family, but its function is unclear. (semanticscholar.org)
  • The Rab family of low-molecular-mass GTP-binding proteins are thought to guide membrane fusion between a transport vesicle and the target membrane, and to determine the specificity of docking(1-3). (ucl.ac.uk)
  • In the cytosol, GDI binds Rab in the GDP-bound form. (genetics.org)
  • Free ribosomes are suspended in the cytosol, while bound ribosomes are attached to the rough endoplasmic reticulum. (cram.com)
  • They can be detected in an inactive or an active conformation depending on the nucleotide‐bound status, and are considered as switches, which cycle between an active, membrane‐associated and an inactive cytosolic status ( Olkkonen and Stenmark, 1997 ). (embopress.org)
  • These domains are occupied by different Rab proteins, revealing compartmentalization within the same continuous membrane. (rupress.org)
  • As each new RAB was discovered, it also became clear that different RAB proteins could adopt specific subcellular localizations, associating with particular membrane. (springer.com)
  • Upstream kinases, LKB1 and Ca 2+ /calmodulin-dependent protein kinase kinase (CaMKK) phosphorylate AMPK T172 in mammalian cells. (koreamed.org)
  • Like that of other members of the Ras superfamily, the nucleotide-dependent switch of Rab proteins is thought to control a downstream catalytic process. (nature.com)
  • The structure explains the nucleotide-independent binding of Rheb to PDEδ and the relaxed specificity of PDEδ. (rcsb.org)
  • One of these residues is located at the N-terminus of the switch I region near the nucleotide binding site whereas the other two flank an exposed hydrophobic triad previously implicated in effector recognition. (umassmed.edu)
  • A small G protein acts as a molecular switch that cycles between inactive GDP-bound and active GTP-bound forms. (ahajournals.org)
  • 5-8 Importantly, recent accumulating evidence highlighted the significance of these small G proteins as essential molecular switches that trigger many of the signal transduction and functions of Ang II. (ahajournals.org)
  • Localisation of low molecular weight GTP binding proteins to exocytic and endocytic compartments. (springer.com)
  • Specifically we have utilized various molecular biology techniques including cDNA transfection of human tyrosinase and tyrosinase-related protein (TRP-1). (nii.ac.jp)
  • Deconstructing the signaling mechanisms modulated by Rab proteins during apoptosis could unveil underlying molecular mechanisms that may be exploited therapeutically to selectively target malignant cells. (edu.au)
  • Among the multiple functions that have been associated with PTMA are cell proliferation, DNA packaging (through its binding to histone H1), activation of transcription, cell survival, and neoplastic transformation, some of which have often been ascribed to molecular mimicry. (unipd.it)
  • This project provided me with the opportunity to gain practical experience and knowledge in protein purification and molecular cloning techniques. (bspp.org.uk)
  • To get further molecular insight about the role of IFT27, GDP- or GTP-locked versions were expressed in presence or absence of endogenous IFT27. (elifesciences.org)
  • IFT trains are made from two different protein complexes called IFT-A and IFT-B, which are dragged by various molecular motors. (elifesciences.org)
  • The methodologies that we employ include: molecular biology, protein:protein interaction technologies, tissue culture, immunofluorescence/confocal microscopy, fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET), biochemical assays, immunoblotting and immunoprecipitation assays. (ucc.ie)
  • While the molecular mechanisms behind this impairment are undefined, it has been predicted that protein retention results from a disruption of vesicle-mediated secretory processes. (elsevier.com)
  • Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily. (semanticscholar.org)
  • Localization of Rab3A-binding site on C2A domain of synaptotagmin I to reveal its regulatory mechanism. (bireme.br)
  • Membrane trafficking mediated by Rab proteins is controlled by intracellular localization of Rab proteins, Rab‐membrane interactions and GTP‐activation processes. (edu.au)
  • A full glycosylation profile of GLUT4 has been shown to be very important for GLUT4 protein stability [136,137] and GLUT4 localization [138]. (diabetesincontrol.com)
  • This chapter discusses the diversification of cysteine proteases (CPs) and Rab in the context of their diverse localization and function. (asmscience.org)
  • These results are supported by immunofluorescence localization of hepatic proteins on liver sections. (elsevier.com)
  • Ras-related protein Rab-21 (RAB21) belongs to the Ras superfamily of small GTP-binding proteins. (prospecbio.com)
  • The organization and regulation of membrane-fusion reactions, then, are crucial for virtually every membrane-bound biological process. (nature.com)
  • The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. (abnova.com)
  • After internalization, the particles become sequestered in membrane-bound organelles, called phagosomes, which undergo a process of maturation that involves acidification and several fusion events. (asm.org)
  • Membrane-bound and secreted proteins are made on ribosomes found in the rough endoplasmic reticulum . (wikipedia.org)
  • membrane bound ribosomes found where? (cram.com)
  • In order to localize Rab3-binding site on C2A and reveal the relevant regulatory mechanism, in the present study we investigated the interaction between recombinant Rab3A and various C2A mutants. (bireme.br)
  • What does rab3 gtp-binding proteins mean? (definitions.net)
  • Here are all the possible meanings and translations of the word rab3 gtp-binding proteins . (definitions.net)
  • Are we missing a good definition for rab3 gtp-binding proteins ? (definitions.net)
  • What rhymes with rab3 gtp-binding proteins ? (definitions.net)
  • The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D. (thermofisher.com)
  • GTP binding YPT1 protein and Ca2+ function independently in a cell-free protein transport reaction. (springer.com)
  • A monoclonal antibody specifically recognizing the 23.5-kd yeast YPT1 protein cross-reacted with a protein of identical size on protein blots of mouse, rat, pig, bovine and human cell lines. (embl.de)
  • The vesicle is drawn into the cell, pinched off from the cell membrane (a process called endocytosis), and attached to the Rab23 protein. (medlineplus.gov)
  • 1998 ). Protein kinase B/akt and Rab5 mediate Ras activation of endocytosis. (biologists.org)
  • In addition to the GSV pool, GLUT4 also cycles through an early endosomal compartment in an endocytotic/exocytotic pathway (distinct from fluid-phase endocytosis) analogous to that described for other insulin-responsive proteins, such as the mannose-6-phosphate/insulin-like growth factor 2 receptor and the transferrin receptor. (diabetesincontrol.com)
  • CELLS constitutively transport newly synthesized proteins, lipids, and other molecules to their periphery through vesicle trafficking. (genetics.org)
  • Chavrier P, Simons K, Zerial M. The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach. (springer.com)
  • PDEδ is a putative solubilizing factor for several prenylated Ras-subfamily proteins. (rcsb.org)
  • Furthermore, our results indicate that the EHBP-1 central calponin homology (CH) domain binds to actin microfilaments in a reaction that is stimulated by RAB-10(GTP). (nih.gov)
  • Through the transport of certain proteins, the Rab23 protein regulates a specific developmental pathway called the hedgehog signaling pathway that is critical in cell growth (proliferation), cell specialization, and the normal shaping (patterning) of many parts of the body during embryonic development. (medlineplus.gov)
  • It was speculated that the interaction between Rab3A and C2A is not simply based on electrostatic force, and Rab3A regulates C2A-mediated vesicle-presynaptic membrane fusion mainly through affecting the C2A binding to phospholipids in the presynaptic membrane. (bireme.br)
  • Here we report that EAT-17 , a novel GTPase-activating protein (GAP), regulates RAB-6.2 function in grinder formation in Caenorhabditis elegans . (genetics.org)
  • The small GTP ase Rab7b regulates autophagic flux by promoting the activity of the cysteine protease Atg4B, the key autophagic enzyme responsible for cleaving the precursor form of LC 3. (embopress.org)
  • 48 h later, bacteria - containing vacuoles were labeled by LysoTracker, a marker of acidic compartments, and accumulated monodansylcadaverine and displayed protein LC3, both markers of autophagic vacuoles. (asm.org)
  • Rab proteins are involved in regulating membrane traffic between various intracellular compartments. (embopress.org)
  • Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. (uniprot.org)
  • We generated mutants that present the inactive (GDP‐bound) and active (GTP‐bound) form of rab1b and used the GTPase‐deficient rab1bQ67R mutant in yeast two‐hybrid screening experiments to identify interacting proteins. (embopress.org)
  • Two of them encode large protein fragments highly homologous to rat GM130 and to human Golgin95. (embopress.org)
  • Synaptotagmin I (Syt I) functions in the regulation of neurotransmitter release and multiple other cellular processes through its C2 domain binding to other molecules. (bireme.br)
  • In this regard, Ras and Rho G proteins are the most investigated molecules in the cardiovascular system. (ahajournals.org)
  • The activity of SNARE molecules is regulated by several soluble cytosolic proteins. (embopress.org)
  • In addition to expanding GTP hydrolytic paradigms, these observations reveal the unconventional dual finger and non-canonical catalytic network mechanisms of Rab GAPs as necessary alternative solutions to a major impediment imposed by substitution of the conserved P-loop glycine. (umassmed.edu)
  • Rab proteins are involved in the regulation of diverse cellular functions associated with intracellular membrane trafficking, including autophagy and immune response to bacterial infection. (genecards.org)
  • Control of Rab GTPase activity is exerted through multiple levels of regulation. (biologists.org)
  • 2 describe an enormous technical accomplishment - the self-assembly of 17 individual purified proteins into a fusion 'machine' whose activity and regulation recapitulate those of membrane fusion in an intact cell. (nature.com)
  • SNARE complexes are structurally similar to viral envelope proteins known to catalyse membrane fusion 6 , so a paradigm emerged casting these rod-shaped helical bundles into the central role of 'minimal fusion machine', with all other proteins assigned to supporting roles such as regulation of the SNARE complex 7 . (nature.com)
  • Regulation of the GTPase activity of the ras like protein p25-rab3a: evidence for a rab3a specific GAP. (springer.com)
  • The proteomic analysis revealed the presence of 21 proteins known to be associated with specific renal diseases or blood pressure regulation. (pnas.org)
  • Mid1ip1L protein localizes to cortical F-actin aggregates, consistent with a direct role in cytoskeletal regulation. (zfin.org)
  • This differential trafficking of RET isoforms produces a more rapid and longer duration of signaling through the extracellular-signal regulated kinase/mitogen-activated protein kinase pathway downstream of RET51 relative to RET9. (biomedsearch.com)
  • 6 Metformin mediates its inhibitory function by activating the tumor suppressor LKB1 and its downstream target AMP-activated protein kinase (AMPK) and by inhibiting the RagGTPases that are essential for mTORC1 activation by amino acids. (pubmedcentralcanada.ca)
  • Recombinant protein corresponding to full length human RAB27A. (abnova.com)
  • Western blot analysis of human RAB27A (AA: 1-221) recombinant protein (Expected MW is 50.8 kDa) with RAB27A monoclonal antibody, clone 7D7C9 (Cat# MAB12243) at 1:500-1:2000 dilution. (abnova.com)
  • Human RAB1A full-length ORF ( NP_004152.1, 1 a.a. - 205 a.a.) recombinant protein with GST-tag at N-terminal. (abnova.com)
  • This RAB3A antibody is generated from a mouse immunized with a recombinant protein. (thermofisher.com)
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)

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