Epididymal Secretory Proteins: Proteins secreted by the epididymal epithelium. These proteins are both tissue- and species-specific. They are important molecular agents in the process of sperm maturation.Factor VIII: Blood-coagulation factor VIII. Antihemophilic factor that is part of the factor VIII/von Willebrand factor complex. Factor VIII is produced in the liver and acts in the intrinsic pathway of blood coagulation. It serves as a cofactor in factor X activation and this action is markedly enhanced by small amounts of thrombin.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Hemophilia A: The classic hemophilia resulting from a deficiency of factor VIII. It is an inherited disorder of blood coagulation characterized by a permanent tendency to hemorrhage.SepharoseAntibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Blood Coagulation: The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.PDZ Domains: Protein interaction domains of about 70-90 amino acid residues, named after a common structure found in PSD-95, Discs Large, and Zona Occludens 1 proteins. PDZ domains are involved in the recruitment and interaction of proteins, and aid the formation of protein scaffolds and signaling networks. This is achieved by sequence-specific binding between a PDZ domain in one protein and a PDZ motif in another protein.Synapses: Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.Dendritic Spines: Spiny processes on DENDRITES, each of which receives excitatory input from one nerve ending (NERVE ENDINGS). They are commonly found on PURKINJE CELLS and PYRAMIDAL CELLS.Oncogene Fusion: The GENETIC RECOMBINATION of the parts of two or more GENES, including an ONCOGENE as at least one of the fusion partners. Such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS.Ankyrin Repeat: Protein motif that contains a 33-amino acid long sequence that often occurs in tandem arrays. This repeating sequence of 33-amino acids was discovered in ANKYRIN where it is involved in interaction with the anion exchanger (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE). Ankyrin repeats cooperatively fold into domains that mediate molecular recognition via protein-protein interactions.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Crotalid Venoms: Venoms from snakes of the subfamily Crotalinae or pit vipers, found mostly in the Americas. They include the rattlesnake, cottonmouth, fer-de-lance, bushmaster, and American copperhead. Their venoms contain nontoxic proteins, cardio-, hemo-, cyto-, and neurotoxins, and many enzymes, especially phospholipases A. Many of the toxins have been characterized.Anopheles gambiae: A species of mosquito in the genus Anopheles and the principle vector of MALARIA in Africa.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Bacterial Proteins: Proteins found in any species of bacterium.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Phosphorus-Oxygen Lyases: Enzymes that catalyze the cleavage of a phosphorus-oxygen bond by means other than hydrolysis or oxidation. EC 4.6.alpha-Synuclein: A synuclein that is a major component of LEWY BODIES that plays a role in neurodegeneration and neuroprotection.Presynaptic Terminals: The distal terminations of axons which are specialized for the release of neurotransmitters. Also included are varicosities along the course of axons which have similar specializations and also release transmitters. Presynaptic terminals in both the central and peripheral nervous systems are included.Nerve Endings: Branch-like terminations of NERVE FIBERS, sensory or motor NEURONS. Endings of sensory neurons are the beginnings of afferent pathway to the CENTRAL NERVOUS SYSTEM. Endings of motor neurons are the terminals of axons at the muscle cells. Nerve endings which release neurotransmitters are called PRESYNAPTIC TERMINALS.Synaptosomes: Pinched-off nerve endings and their contents of vesicles and cytoplasm together with the attached subsynaptic area of the membrane of the post-synaptic cell. They are largely artificial structures produced by fractionation after selective centrifugation of nervous tissue homogenates.Synucleins: A family of homologous proteins of low MOLECULAR WEIGHT that are predominately expressed in the BRAIN and that have been implicated in a variety of human diseases. They were originally isolated from CHOLINERGIC FIBERS of TORPEDO.Synaptic Vesicles: Membrane-bound compartments which contain transmitter molecules. Synaptic vesicles are concentrated at presynaptic terminals. They actively sequester transmitter molecules from the cytoplasm. In at least some synapses, transmitter release occurs by fusion of these vesicles with the presynaptic membrane, followed by exocytosis of their contents.Neuromuscular Junction: The synapse between a neuron and a muscle.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Fruiting Bodies, Fungal: The fruiting 'heads' or 'caps' of FUNGI, which as a food item are familiarly known as MUSHROOMS, that contain the FUNGAL SPORES.Molecular Weight: The sum of the weight of all the atoms in a molecule.Connective Tissue Diseases: A heterogeneous group of disorders, some hereditary, others acquired, characterized by abnormal structure or function of one or more of the elements of connective tissue, i.e., collagen, elastin, or the mucopolysaccharides.Bone Morphogenetic Protein 2: A potent osteoinductive protein that plays a critical role in the differentiation of osteoprogenitor cells into OSTEOBLASTS.Collagen Diseases: Historically, a heterogeneous group of acute and chronic diseases, including rheumatoid arthritis, systemic lupus erythematosus, progressive systemic sclerosis, dermatomyositis, etc. This classification was based on the notion that "collagen" was equivalent to "connective tissue", but with the present recognition of the different types of collagen and the aggregates derived from them as distinct entities, the term "collagen diseases" now pertains exclusively to those inherited conditions in which the primary defect is at the gene level and affects collagen biosynthesis, post-translational modification, or extracellular processing directly. (From Cecil Textbook of Medicine, 19th ed, p1494)Connective Tissue: Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.Bone Morphogenetic Protein 7: A bone morphogenetic protein that is widely expressed during EMBRYONIC DEVELOPMENT. It is both a potent osteogenic factor and a specific regulator of nephrogenesis.Bone Morphogenetic Proteins: Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.Soft Tissue Injuries: Injuries of tissue other than bone. The concept is usually general and does not customarily refer to internal organs or viscera. It is meaningful with reference to regions or organs where soft tissue (muscle, fat, skin) should be differentiated from bones or bone tissue, as "soft tissue injuries of the hand".Cat's Claw: A vine (Uncaria tomentosa) indigenous to the Amazon rainforest whose name is derived from its hook-like thorns. It contains oxindole alkaloids and glycosides and has many medicinal uses.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Togaviridae: A family of RNA viruses, mainly arboviruses, consisting of two genera: ALPHAVIRUS (group A arboviruses), and RUBIVIRUS. Virions are spherical, 60-70 nm in diameter, with a lipoprotein envelope tightly applied to the icosahedral nucleocapsid.Anterior Thalamic Nuclei: Three nuclei located beneath the dorsal surface of the most rostral part of the thalamus. The group includes the anterodorsal nucleus, anteromedial nucleus, and anteroventral nucleus. All receive connections from the MAMILLARY BODY and BRAIN FORNIX, and project fibers to the CINGULATE BODY.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Los AngelesMamillary Bodies: A pair of nuclei and associated gray matter in the interpeduncular space rostral to the posterior perforated substance in the posterior hypothalamus.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Leukemia, Experimental: Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.Leukemia Virus, Murine: Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
De Silva B.S.; Egodage K.L.; Wilson G.S. (1999). "Purified protein derivate (PPD) as an immunogen carrier elicits high antigen ... Ovalbumin (OVA) Also known as egg albumin, OVA is the main protein (60-75%) found in hen egg white. OVA is soluble in Dimethyl ... doi:10.1016/s0968-4328(99)00036-0. Arancibia S.; Del Campo M.; Nova E.; Salazar F.; Becker M.I. (2012). "Enhanced structural ... The cationized form of BSA (cBSA) is highly positively charged protein with significantly increased immunogenicity. This change ...
... -like protein is a protein that in humans is encoded by the RRP15 gene. This gene encodes a protein that co-purifies with ... De Marchis ML, Giorgi A, Schinina ME, Bozzoni I, Fatica A (Mar 2005). "Rrp15p, a novel component of pre-ribosomal particles ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... A similar protein in budding yeast is a component of pre-60S ribosomal particles, and is required for the early maturation ...
... determination of complete genomic sequence and characterization of purified recombinant protein". Archives of Biochemistry and ... Hoch U, Ortiz De Montellano PR (Apr 2001). "Covalently linked heme in cytochrome p4504a fatty acid hydroxylases". The Journal ... Cytochrome P450 4A11 is a protein that in humans is encoded by the CYP4A11 gene. This gene encodes a member of the cytochrome ... This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate. ...
Nash RA, Caldecott KW, Barnes DE, Lindahl T (April 1997). "XRCC1 protein interacts with one of two distinct forms of DNA ligase ... "Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 ... Nuclear DNA ligase III-alpha exists and functions in a stable complex with the DNA repair protein XRCC1. These proteins ... Barnes DE, Tomkinson AE, Lehmann AR, Webster AD, Lindahl T (May 1992). "Mutations in the DNA ligase I gene of an individual ...
"Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 ... Kubota Y, Nash RA, Klungland A, Schär P, Barnes DE, Lindahl T (Dec 1996). "Reconstitution of DNA base excision-repair with ... purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein". The EMBO Journal. 15 (23): 6662-70. ... Yeast three-hybrid assays have shown that this protein binds to the stem loops within the 3'UTR of the POLB mRNA, however the ...
Nash RA, Caldecott KW, Barnes DE, Lindahl T (Apr 1997). "XRCC1 protein interacts with one of two distinct forms of DNA ligase ... "Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 ... DNA repair protein XRCC1 also known as X-ray repair cross-complementing protein 1 is a protein that in humans is encoded by the ... The XRCC1 protein does not have enzymatic activity, but acts as a scaffolding protein that interacts with multiple repair ...
De Lumen (April 2013). "Chemopreventive properties of Peptide Lunasin: a review". Protein Pept. Lett. (Review). 20 (4): 424-32 ... This limitation has been overcome by the development of methods to isolate highly purified lunasin from soybean white flake, a ... De Lumen, BO (2008). "Lunasin: A novel cancer preventive seed peptide that modifies chromatin". Journal of AOAC International. ... The name of the protein was chosen from the Filipino word lunas, which means "cure". Lunasin was patented as a biologic ...
Using the "SAXS filter", the authors were able to purify the set of de novo protein models significantly. This was further ... X-ray crystallography Electron microscopy Neutron spin echo Protein Data Bank Protein dynamics Protein folding Protein ... The authors aimed to improve results of fold recognition and de novo protein structure prediction methods. SAXS data provide ... doi:10.1093/protein/gzi026. PMID 15845555. Zheng W, Doniach S (2002). "Protein structure prediction constrained by solution X- ...
The last protein unit is unique and is known as the A subunit. The A subunit of an AB5 toxin is the portion responsible for ... Sambhu Nath De. He conducted his research in Calcutta (now Kolkata) making his discovery in 1959, although it was first ... purified by Robert Koch in 1883. Cholera toxin is an infectious toxin composed of a protein complex that is secreted by the ... Cholera toxin, shiga toxin, and SubAB toxin all have B subunits that are made up of five identical protein components, meaning ...
It was determined later to contain a large amount of protein. 1889: Hugo de Vries postulates that "inheritance of specific ... Richard Altmann purified protein free DNA. However, the nucleic acid was not as pure as he had assumed. ... "protein foreign to a unicellular organism." These two scientist were able to replicate proteins such as HGH, Erythropoietin and ... Hugo de Vries wondered what the nature of germ plasm might be, and in particular he wondered whether or not germ plasm was ...
... she identified and purified the Telomeric-repeat binding factor protein 1 (TRF1). With the assistance of Bas van Steensel, de ... In 2005, de Lange came to the crucial realization that six telomeric proteins form a dynamic protein complex, that she named ... T. de Lange (2005) Shelterin: the protein complex that shapes and safeguards human telomeres. Genes and Development 19: 2100- ... Titia de Lange attended University of Amsterdam where she received her bachelor's and master's degree in biochemistry. De Lange ...
Subcutaneous Immunization with Inactivated Bacterial Components and Purified Protein of Escherichia coli, Fusobacterium ... The Merck Veterinary Manual, "Metritis and Endometritis" Vinícius Silva Machado, Marcela Luccas de Souza Bicalho u. a.: ...
De Camilli P, Cameron R, Greengard P (May 1983). "Synapsin I (protein I), a nerve terminal-specific phosphoprotein. I. Its ... protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified ... Originally named Protein I, it was found as an endogenous substrate for cAMP-dependent protein kinase in the synaptic membrane ... Cyclic AMP-dependent protein kinase is unique in its mechanism of activation. The protein kinase is composed of two regulatory ...
Alberts decided to do something that no one else was doing, and developed a DNA column for the purification of proteins that ... This enabled him to purify T4 Bacteriophage Gene 32. In 1966, Alberts joined the Department of Biochemical Sciences at ... Alberts went to the Institut de Biologie Moleculaire at the University of Geneva as a postdoctoral fellow, and worked with ... Also in 1976, he and his students were able to add all seven proteins to double-stranded DNA in an appropriate magnesium ...
De Stefano V, Mastrangelo S, Schwarz HP, et al. (August 1993). "Replacement therapy with a purified protein C concentrate ... Protein C is an innate anticoagulant, and as warfarin further decreases protein C levels, it can lead to massive thrombosis ... June 1993). "Treatment of coumadin-induced skin necrosis with a monoclonal antibody purified protein C concentrate". Arch ... There have also been cases in patients with other deficiency, including protein S deficiency, activated protein C resistance ( ...
De's desire to purify the cholera toxin did not progress any further as the protein purification technology was not well ... His father Mr Dasarathi De was a not so successful businessman. Supported by his uncle Asutosh De, De completed the ... De, S. N. Cholera: its pathology and pathogenesis, Published by Oliver and Boyd, London, 1961. De, S. N. and Chatterje, D. N. ... After his return, De worked on pathogenesis of cholera and started publishing his findings. In 1955, De became the Head of ...
These include, for example, coiled-coil proteins with structural functions and regulatory proteins, such as components of the ... In cell biology, microtubule nucleation is the event that initiates de novo formation of microtubules (MTs). These filaments of ... Microtubule nucleation occurs spontaneously in vitro, with solutions of purified tubulin giving rise to full-length polymers. ... Though the γ-TuRC is the primary protein cells turn to when faced with the task of nucleating microtubules, it is not the only ...
Most successful protein designs have involved globular proteins. Both RSD-1, and Top7 were de novo designs of globular proteins ... Membrane proteins are hard to purify. Their structure is hard to characterize because they adopt their native conformation only ... Protein-protein interactions can be designed using protein design algorithms because the principles that rule protein stability ... proteins can be redesigned from the sequence and structure of a known protein, or completely from scratch in de novo protein ...
... protein shake in Vanilla, Strawberry and Chocolate by Tropic SA Cola Couronne - fruit champagne by the Brasserie de la Couronne ... spring water purified by reverse osmosis and ozonized by Tropic SA Big Shake - ... Ser Spring Up Torasso Tradicional de Secco Secco Tubito Villa del Sur Levité Back o' Bourke Cordials - Range of popular drinks ... de:Vita Cola "Gletscher Cola". "Egils Soft Drinks". Nammi.is, Icelandic Products Online Shop. Archived from the original on ...
... and the protein can be purified from cultured parasites. Meissner, M; Krejany, E; Gilson, PR; De Koning-Ward, TF; Soldati, D; ... By virtue of protein being tagged to a DHFR degradation domain from E. coli, as well as GFP and a HA-tag, protein levels can be ... Muralidharan, V; Goldberg, DE (2011). "Asparagine repeat function in a Plasmodium falciparum protein assessed via a regulatable ... Armstrong, CM; Goldberg, DE (2007). "An FKBP destabilization domain modulates protein levels in Plasmodium falciparum". Nature ...
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single ... Abgerufen von „https://de.wikipedia.org/w/index.php?title=Protein-Tag&oldid=170934418" ... ein C-terminales Protein-Tag am Protein während der Translation. Gelegentlich muss das Protein-Tag vom Protein nach der ... Zur Erzeugung eines Protein-Tags wird die codierende DNA-Sequenz des Protein-Tags unter Erhalt des Leserasters in die ...
De Pauw P, Neyt C, Vanderwinkel E, et al. (1995). "Characterization of human serum N-acetylmuramyl-L-alanine amidase purified ... PGLYRP2 is a gene encoding a protein with N-acetylmuramoyl-L-alanine amidase activity. Peptidoglycan recognition proteins, such ... 2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and ... Wang H, Gupta D, Li X, Dziarski R (2005). "Peptidoglycan recognition protein 2 (N-acetylmuramoyl-L-Ala amidase) is induced in ...
"Parathyroid hormone-related protein purified from a human lung cancer cell line". Proceedings of the National Academy of ... Mangin M, Webb AC, Dreyer BE, Posillico JT, Ikeda K, Weir EC, Stewart AF, Bander NH, Milstone L, Barton DE (January 1988). " ... Parathyroid hormone-related protein (or PTHrP) is a protein member of the parathyroid hormone family. It is occasionally ... Jans DA, Thomas RJ, Gillespie MT (2003). "Parathyroid hormone-related protein (PTHrP): a nucleocytoplasmic shuttling protein ...
... lipoyl-carrier protein]-N6-octanoyl-L-lysine This enzyme is purified from the bacterium Bacillus subtilis. Christensen, Q.H.; ... Martin, N.; Christensen, Q.H.; Mansilla, M.C.; Cronan, J.E.; de Mendoza, D. (2011). "A novel two-gene requirement for the ... Octanoyl-(GcvH):protein N-octanoyltransferase (EC 2.3.1.204, LIPL, octanoyl-[GcvH]:E2 amidotransferase, YWFL (gene)) is an ... Octanoyl-(GcvH):protein N-octanoyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular ...
... acyl-carrier protein] + CoA + CO2 This enzyme is purified from the bacterium Pseudomonas aeruginosa PAO1. Yuan, Y.; Leeds, J.A ... Meredith, T.C. (2012). "Pseudomonas aeruginosa directly shunts β-oxidation degradation intermediates into de novo fatty acid ... Beta-ketodecanoyl-(acyl-carrier-protein) synthase (EC 2.3.1.207) is an enzyme with systematic name octanoyl-CoA:malonyl-(acyl- ... Beta-ketodecanoyl-(acyl-carrier-protein) synthase at the US National Library of Medicine Medical Subject Headings (MeSH) ...
"Mémoires de l'Académie Royale des Sciences (in French): 65-68.. *^ a b Weeks, Mary Elvira (1932). "The discovery of the ... The balance between potassium and sodium is maintained by ion transporter proteins in the cell membrane.[231] The cell membrane ... France discovered francium in 1939 by purifying a sample of actinium-227, which had been reported to have a decay energy of 220 ... Petalite (Li Al Si4O10) was discovered in 1800 by the Brazilian chemist José Bonifácio de Andrada in a mine on the island of ...
21.09.2020: Playback of the Electrophysiological investigation of integral membrane proteins. Webinar now available ... 2020 - Calcium activates purified human TRPA1 with and without its N-terminal ankyrin repeat domain in the absence of ... a direct calmodulin-independent action of calcium on the purified human TRPA1 (hTRPA1), causing structural changes and ...
... affecte plus de 1,75 millions de personnes aux Etats-Unis. Puisque la population vieillit, ce numéro grimpera jusquà presque 3 ... Purifying Proteins from Mammalian Cell Culture. Dennis Karthaus, MSc. In many cases mammalian cells are the only option to ... à linstitut doeil de Roski dUniversité de Californie du Sud à lÉcole de Médecine de Keck du campus. ... éviter toute autre perte de ces cellules de lumière-détection, » Clegg expliqué, qui retient la présidence de famille de Wilcox ...
Purifying Proteins from Mammalian Cell Culture. Dennis Karthaus, MSc. In many cases mammalian cells are the only option to ... van de cel worden bekend - van de verstoring in de vorming van rode bloedcellen die in patiënten met DBA worden gevonden de ... De bevindingen van de studie tonen aan dat de totale ribosoomniveaus in combinatie met bepaalde structurele elementen van ... hoe de ontwikkeling van de types van bloedcel na de originele genetische informatie is getranscribeerd in boodschappersRNA ...
Rieder HL, Cauthen GM, Kelly GD, Bloch AB, Snider DE. Tuberculosis in the United States. JAMA 1989;262:385-9. ... Purified Protein Derivative (PPD)-Tuberculin Anergy and HIV Infection: Guidelines For Anergy Testing And Management Of Anergic ... of purified protein derivative {PPD}-tuberculin). Those persons who have at least a 5 mm reaction to PPD should be considered ...
Binding Assays Using Purified Proteins:. Experiments were conducted mainly as described (Dumont, B., et al., Chimeric Fc ... Binding Assays Using Purified Proteins:. Experiments were conducted mainly as described (Dumont B, Minullina I, Loyau S, ... Institut National de la Sante et de la Recherche Medicale (INSERM) (Paris, FR) Universite Paris Diderot (Paris, FR) ... GPVI-Fc and GPVI-His were produced in HEK cells and affinity purified on Protein A and Ni columns respectively. Their affinity ...
Evaluacion epidemiologica y operacional del Programa Nacional de Control de Tuberculosis en el Peru al Año 2000, Informe Anual ... Background: The purified protein derivative (PPD) skin test is the only widely used method which detects latent tuberculosis ... Attenuated response to purified protein derivative in patients with rheumatoid arthritis: study in a population with a high ... Attenuated response to purified protein derivative in patients with rheumatoid arthritis: study in a population with a high ...
SecB protein stabilizes a translocation-competent state of purified prePhoE protein.. Kusters R, de Vrije T, Breukink E, de ... Involvement of membrane lipids in protein export in Escherichia coli.. Tommassen J, de Vrije T, de Cock H, Bosch D, de Kruijff ... Keukens EA, de Vrije T, Jansen LA, de Boer H, Janssen M, de Kroon AI, Jongen WM, de Kruijff B. ... Keukens EA, de Vrije T, van den Boom C, de Waard P, Plasman HH, Thiel F, Chupin V, Jongen WM, de Kruijff B. ...
The recombinant protein was purified to homogeneity and submitted to SDS/PAGE. As depicted in Fig. 1A, a single band displaying ... Purified A. tumefaciens GS Synthesizes Glycogen de Novo. To study the possibility that GS could be acting both as primer and ... linked glucans formed were still linked to a protein moiety. Protein-linked but not protein-free glycans enter into gels (14). ... To whom correspondence should be addressed at: Instituto de Investigaciones Biotecnológicas, Universidad de San Martin, ...
The OCP was further purified on a Whatman DE-52 cellulose column. To quantify the OCP present in the different strains, ... 2007) Protein-protein interactions in carotenoid triggered quenching of phycobilisome fluorescence in Synechocystis sp PCC 6803 ... 1986) Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins. Adv Protein Chem 38:181-364. ... In the light-minus-dark FTIR spectra, these protein contributions, with specific frequencies for each type of protein secondary ...
Purified NCYM proteins were pulled down with GST-fused GSK3β and MYCN. (I) In vitro kinase assay. Radiolabeled ATP was used for ... Recently, de novo gene birth from non-genic DNA was found to be an alternative mechanism to generate novel protein-coding genes ... Collectively, our results show that NCYM is the first de novo evolved protein known to act as an oncopromoting factor in human ... NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3β resulting in the stabilization of ...
A method of preparing high purity procoagulant protein comprising the steps of (a) adsorbing a VIII:C/VIII:RP complex from a ... Compositions containing highly purified factor IX proteins prepared by immunoaffinity chromatography. US5618788 *. 20. Aug. ... Centre Regional De Transfusion Sanguine De Lille. Process for preparing a concentrate of blood coagulation factor VIII-von ... Protein S polypeptides and anti-peptide antibodies that inhibit protein S binding to C4B binding protein, diagnostic systems ...
De Camilli P. (1983) Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic ... Specific antibodies were affinity-purified using SulfoLink columns (Pierce). The specificity of the TANC antibodies (1609 and ... TANC1 is a PSD-95-interacting synaptic protein that contains multiple domains for protein-protein interactions but whose ... although fusion proteins are generally more prone to protein degradation, and these fusion proteins contain little or none of ...
De Camilli P. (1983) Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic ... Antibodies (anti-GRIP1, anti-GRIP2) were affinity purified using BSA-conjugated antigen peptides cross-linked to Affi-Gel 10 ... Recently, proteins that contain the protein-protein interaction motifs called postsynaptic density-95/Discs large/zona ... Whether membrane association is via protein-protein interactions with membrane proteins or by other mechanisms, such as ...
... proteins were subsequently purified using fast protein liquid chromatography. All proteins were purified to ,95% purity. ... Protein purity was assessed using capillary electrophoresis (Bioanalyzer; Agilent Technologies, Wilmington, DE). The protein ... Garcia, B., C. Latasa, C. Solano, F. Garcia-del Portillo, C. Gamazo, and I. Lasa. 2004. Role of the GGDEF protein family in ... The full-length YahA protein was overexpressed as a YahA::His6 fusion and was purified to apparent homogeneity (Fig. 1). Its ...
Benovic for purified human GRK6A protein. Funding: Agence Nationale de la Recherche, France (E. Bezard); Biothèque Primate- ... Cellular regulation of RGS proteins: Modulators and integrators of G protein signaling. Pharmacol. Rev. 54, 527-559 (2002).. ... regulatory proteins) to phosphorylated receptors blocks further G protein activation and initiates receptor internalization (9 ... The G-protein-coupled receptor kinases βARK1 and βARK2 are widely distributed at synapses in rat brain. J. Neurosci. 12, 4045- ...
Antibody was pulled down with magnetic protein G agarose microspheres (ReSyn Biosciences). After elution and de-crosslinking, ... Primary bone marrow-derived macrophages were purified and cultured in growth media (DMEM containing 4,500 mg/l glucose, 110 mg/ ... Surebeads protein G magnetic beads, Bio-Rad; MagReSyn Protein A, ReSyn Biosciences) for 45 minutes at 4°C. Beads were washed ... Differentially expressed (DE) genes were reported for q ≤ 0.05 and fold change of 2 (Figure 1) or q ≤ 0.1 (Figure 3) and were ...
Recombinant CRISPR-Cas9 Protein. Spezies: Streptococcus pyogenes (S. pyogenes). Quelle: Escherichia coli (E. coli). Jetzt ... GenCrispr Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli that can be used for ... antikoerper-online.de (deutsch) *antibodies-online. * antikoerper-online.de (deutsch) * antibodies-online.com (english) ... Protein-Typ Recombinant Biologische Aktivität Active Applikation Antibody Production (AbP), Genome Editing with Engineered ...
Pellino E3 Ubiquitin Protein Ligase Family Member 3 (PELI3) Peptid. Spezies: Human. Quelle: Synthetic. Jetzt Produkt ABIN981579 ... antikoerper-online.de (deutsch) *antibodies-online. * antikoerper-online.de (deutsch) * antibodies-online.com (english) ... Purified Antigendetails Handhabung Anwendungsinformationen zurück nach oben Antigendetails Anzeigen Verstecken Proteinname ... Pellino E3 Ubiquitin Protein Ligase Family Member 3 (PELI3) Peptid Pellino E3 Ubiquitin Protein Ligase Family Member 3 (PELI3) ...
The Structure of Protein Molecules. A protein molecule is composed of thousands of atoms. How are they arranged? Progress has ... Many metals are now purified in the search for new materials to meet the demands of technology ... By Grard de Vaucouleurs. Pure Metals. When almost all impurities are removed from a metal, it usually exhibits unexpected ... been made toward answering the question for fibrous proteins such as hair, horn, fingernail and porcupine quill ...
This protein is a component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine.,, ... genes are small GTP-binding proteins of the RAS superfamily. ARL2 is a member of a functionally distinct group of ARF-like ... This protein is a component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. ... The ADP-ribosylation factor (ARF) genes are small GTP-binding proteins of the RAS superfamily. ARL2 is a member of a ...
... onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal ... Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, ... With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The ... onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal ...
None of the proteins studied exhibited a differential laminar distribution. Since SV2B is reportedly expressed by glutamatergic ... None of the proteins studied exhibited a differential laminar distribution. Since SV2B is reportedly expressed by glutamatergic ... Immunoisolation studies showed that the expression levels of the protein isoforms varied in the three populations of SVs. ... Immunoisolation studies showed that the expression levels of the protein isoforms varied in the three populations of SVs. ...
Protein Extraction, Cy Dye Labeling, and 2-DE-. Proteins were precipitated in a phenol extraction-ammonium precipitation ... The purified peroxisomes were obtained from the interface between 28 and 50% iodixanol. The activities of the following marker ... Representative 2-DE Cy dye-stained protein gels with circles marking those proteins differentially expressed in each experiment ... By comparing all 2-DE maps, we obtained a minimal set of proteins with an altered expression profile. This group of proteins ...
DNA molecules encoding the BMP-16 proteins are also disclosed. The proteins may be used in the treatment of bone, cartilage, ... The proteins may also be useful for the induction of growth and/or differentiation of undifferentiated embryonic and stem cells ... Purified BMP-16 proteins and processes for producing them are disclosed. ... P gina principal de Google - Sitemap - Descargas en bloque de USPTO - Pol tica de Privacidad - Condiciones de Servicio - Acerca ...
  • 21.09.2020: Playback of the Electrophysiological investigation of integral membrane proteins. (nanion.de)
  • Here, we demonstrate based on Förster resonance energy transfer (FRET) and bilayer patch-clamp studies, a direct calmodulin-independent action of calcium on the purified human TRPA1 (hTRPA1), causing structural changes and activation without immediate subsequent desensitization of hTRPA1 with and without its N-terminal ankyrin repeat domain (N-ARD). (nanion.de)
  • MYCN directly transactivates both NCYM and MYCN mRNA, whereas NCYM stabilizes MYCN protein by inhibiting the activity of GSK3β, a kinase that promotes MYCN degradation. (nih.gov)
  • TANC1 proteins are localized at excitatory synapses, directly interact with PSD-95, and form a complex with diverse excitatory postsynaptic proteins including PSD-95, GKAP, AMPA receptors, NMDA receptors, and the α subunit of Ca 2+ /calmodulin-dependent protein kinase II. (jneurosci.org)
  • With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. (biomol.com)
  • A dynamic mechanism is described here in which the adaptor protein, growth factor receptor-bound protein 2 (Grb2), controls fibroblast growth factor receptor 2 (FGFR2) signaling by regulating receptor kinase and SH2 domain-containing protein tyrosine phosphatase 2 (Shp2) phosphatase activity in the absence of extracellular stimulation. (rupress.org)
  • Release of the interaction with Grb2 permits the FGFR2 kinase domain to access additional tyrosine residues on the receptor, and to recruit downstream effector proteins required for signal transduction. (rupress.org)
  • Protein structure prediction showed that there was one protein kinase C phosphorylation site, four casein kinase II phosphorylation sites and one amidation site in the POLR2H protein, further shaping advanced protein structure. (embl-heidelberg.de)
  • 2. A substantially isolated protein having the kinase activity of the protein shown in SEQ ID NO:2, which is encoded by a nucleotide sequence that hybridizes to SEQ ID NO:1 under highly stringent conditions. (google.de)
  • The same authors recently reported that the chemokines, interferon-inducible protein 10 (IP-10) and MIP-1α, block the stimulatory effects of GM-CSF and SCF on MAP kinase activity in MO7e cells. (bloodjournal.org)
  • This was followed by translocation of the components of antiviral RNA recognition machinery, including RIG-I (retinoic acid-inducible protein I), TRADD (TNFR1-associated death domain protein), TRIM25 (tripartite motif protein 25), and IKKε (inducible IκB kinase), onto the mitochondria. (jimmunol.org)
  • Activator of protein kinase G type I α and type II. (biolog.de)
  • Caution: Protein kinase A type II is activated as well. (biolog.de)
  • In addition, a subset of Srb/Ssn proteins was identified as the distinct kinase module whose association with core Mediator is known to suppress the positive function of Mediator [ 9 , 10 ]. (hindawi.com)
  • We show that oomycete-derived Nep1 (for necrosis and ethylene-inducing peptide1)-like proteins (NLPs) trigger a comprehensive immune response in Arabidopsis thaliana , comprising posttranslational activation of mitogen-activated protein kinase activity, deposition of callose, production of nitric oxide, reactive oxygen intermediates, ethylene, and the phytoalexin camalexin, as well as cell death. (plantcell.org)
  • SOURCE: Biochimie 1988 Dec;70(12):1759-64 ABSTRACT: Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent hmogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. (madsci.org)
  • Thus, these results suggest that GSK3β inhibition by NCYM stabilizes the MYCN protein both in vitro and in vivo. (nih.gov)
  • Intracellular membrane fusion has been mimicked in vitro using a mix of 17 purified proteins and lipid bilayers. (nature.com)
  • Polysaccharides purified from large-scale cell cultures of the plant Echinacea purpurea were tested for their ability to activate human phagocytes in vitro and in vivo. (fraunhofer.de)
  • Over recent years, macrophage inflammatory protein-1α (MIP-1α), which is constitutively 1 and inducibly 2 produced by stromal macrophages and myoid cells 3 and is a member of a family of closely related small (8 to 12 kD) proteins called chemokines, 4-6 has been shown to inhibit cell cycling of primitive hematopoietic cells in vitro 7 8 and in vivo. (bloodjournal.org)
  • A captação de glicose in vitro e conteúdo do GLUT- 4, que indicam atividade da via glicolítica foram reduzidos no EPI e RETRO, assim como a gliceroneogênese avaliada pela incorporação de 1-14C-piruvato em TAG, sem alterações na atividade e conteúdo da fosfoenolpiruvato carboxiquinase (PEPCK). (usp.br)
  • A atividade lipolítica basal foi avaliada in vitro pela liberação de glicerol por adipócitos isolados, e não foi alterada pela ingestão de dieta HL, porém quando estimulada por noradrenalina a liberação de glicerol foi menor nos animais HL, assim como as fosforilações da ATGL e HSL e conteúdo do receptor adrenérgico ?3. (usp.br)
  • Promoter-specific mRNA synthesis requires a minimal set of proteins comprising Pol II (RNA polymerase II) and associated GTFs (general transcription factors), which is defined as in vitro "basal transcription" [ 1 ]. (hindawi.com)
  • Regulated responses of this minimal transcription system by DNA-binding transcription factors or in vitro transcription from nucleosomal DNA template require additional sets of coregulator proteins involved in chromatin remodeling/modification as well as targeted recruitment of basal transcription machinery to the promoter [ 2 , 3 ]. (hindawi.com)
  • Delivery of bacterial effector proteins into the host cell requires the Dot/Icm type IV secretion system. (asm.org)
  • While the function of these effectors has been well characterized, the role of another Rab1-interacting protein from L. pneumophila , the effector protein LidA, is poorly understood. (asm.org)
  • For that, L. pneumophila delivers over 300 effector proteins across the vacuolar membrane into the host cytosol, where they manipulate host signaling processes ( 17 ). (asm.org)
  • L. pneumophila mutants with a defective Dot/Icm system are unsuccessful in transforming their vacuole into a camouflaged replication compartment and are trafficked along the endolysosomal route for degradation, underscoring the importance of effector proteins for bacterial virulence ( 2 , 5 , 7 , 38 , 40 , 44 , 47 , 51 ). (asm.org)
  • SNARE complexes are structurally similar to viral envelope proteins known to catalyse membrane fusion 6 , so a paradigm emerged casting these rod-shaped helical bundles into the central role of 'minimal fusion machine', with all other proteins assigned to supporting roles such as regulation of the SNARE complex 7 . (nature.com)
  • They are created de novo by mutations within a coding sequence that lead to the expression of a novel protein in another reading frame, a process called "overprinting. (asm.org)
  • By analyzing the phylogenetic distribution of overlapping proteins, we were able to confirm that 17 of these had been created de novo and to study them individually. (asm.org)
  • Most proteins created de novo are orphans (i.e., restricted to one species or genus). (asm.org)
  • Most proteins created de novo are predicted to be fully disordered and have a highly unusual sequence composition. (asm.org)
  • Some proteins created de novo are predicted to be ordered, however, and whenever a three-dimensional structure of such a protein has been solved, it corresponds to a fold previously unobserved, suggesting that the study of these proteins could enhance our knowledge of protein space. (asm.org)
  • The resulting overlap encodes an ancestral, "overprinted" protein region and a protein region created de novo (i.e., not by duplication) called an "overprinting" or "novel" region (Fig. 1 ). (asm.org)
  • However, this belief might actually reflect the fact that proteins created de novo are in general very difficult to identify ( 55 ). (asm.org)
  • Indeed, a long-standing question is whether a protein that has no detectable homolog in other organisms (called an "orphan" protein or "ORFan" [ 27 ] or "taxonomically restricted" [ 110 ]) represents a protein created de novo in a particular organism or merely a protein that is a member of a larger family whose other members have diverged beyond recognition or have become extinct ( 115 ). (asm.org)
  • Proteins created de novo by overprinting provide a valuable opportunity to address these questions, and this constitutes one of the two strands of our study. (asm.org)
  • About half of all biological processes involve membrane proteins, which must be delivered, and eventually removed, with great accuracy to regulate the constancy of structural identity. (nature.com)
  • These include the SNARE family of membrane proteins and the Rab proteins, a subfamily of the Ras superfamily of GTPase enzymes. (nature.com)
  • However, direct tests comparing the contributions of SNAREs alone with SNAREs plus all the other proteins essential for fusion have not been performed because of the complexities of assembling microgram quantities of membrane proteins in defined lipid environments together with other fragile purified proteins - requirements that stretch the limits of current biochemical technologies. (nature.com)
  • 21.09.2020: Playback of the Electrophysiological investigation of integral membrane proteins. (nanion.de)
  • In many cases mammalian cells are the only option to produce recombinant proteins with correct post-translational modifications, e.g. glycosylation, which are required for proper function of the therapeutic protein. (news-medical.net)
  • It was concluded that, contrary to what happens in mammalian and yeast cells in which two different proteins are required for linear α(1,4)-linked glucan formation (glycogenin for initiation and GS for further elongation), in A. tumefaciens and probably in all other bacteria, the same protein is involved in both glycogen initiation and elongation. (pnas.org)
  • Mass-spectrometry-based proteomics has allowed the identification of the entire protein complement of yeast and the close-to-complete set of proteins expressed in mammalian cell lines. (mcponline.org)
  • Rab proteins alternate between GDP-bound and GTP-bound forms, a switch controlled by proteins called guanine-nucleotide exchange factors. (nature.com)
  • Like that of other members of the Ras superfamily, the nucleotide-dependent switch of Rab proteins is thought to control a downstream catalytic process. (nature.com)
  • Here, we show that LidA binding to Rab1 stabilized the Rab1-guanosine nucleotide complex, protecting it from inactivation by GTPase-activating proteins (GAPs) and from nucleotide extraction. (asm.org)
  • TANC1 is a PSD-95-interacting synaptic protein that contains multiple domains for protein-protein interactions but whose function is not well understood. (jneurosci.org)
  • α-synuclein's physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. (biolegend.com)
  • We investigated whether cortical glutamatergic and GABAergic release machineries can be differentiated on the basis of the nature and amount of proteins they express, by performing a quantitative analysis of the degree of co-localization of synaptotagmin (SYT) 1 and 2, synaptic vesicle protein 2 (SV2) A and B, and Rab3a and c in VGLUT1+, VGLUT2+, and VGAT+ terminals and synaptic vesicles (SVs) in rat cerebral cortex. (frontiersin.org)
  • To further define the complement of proteins participating in transmitter release in GABAergic and glutamatergic terminals, we performed a quantitative analysis of the localization of synaptotagmin (SYT) 1 and 2, synaptic vesicle protein 2 (SV2) A and B, and Rab3a and c in VGLUT1+, VGLUT2+, and VGAT+ terminals and synaptic vesicles (SVs) of the cerebral cortex of adult rats. (frontiersin.org)
  • After fusion at the active zone, vesicles are reformed locally, primarily via clathrin-mediated endocytosis, and then rejoin the synaptic vesicle cluster (for review see Slepnev and De Camilli, 2000 ). (rupress.org)
  • Synapsins are a family of neuron-specific proteins that are bound to synaptic vesicles. (rupress.org)
  • Determinants of liposome fusion mediated by synaptic SNARE proteins. (mpg.de)
  • We accurately measured the absolute protein concentrations of 13 platelet proteins using SILAC-protein epitope signature tags and used them as reference points to estimate the copy numbers of all proteins of the platelet proteome. (mcponline.org)
  • Succinoglycan purified from cultures was applied to different concentrations of cells, and observation of the phase behaviour showed that the limiting polymer concentration for aggregation and phase separation to occur decreased with increasing cell concentration, suggesting a 'crowding mechanism' was occurring. (royalsocietypublishing.org)
  • Author affiliations: Centre National de la Recherche Scientifique, Bank accession no. (cdc.gov)
  • The EAL domain (also known as domain of unknown function 2 or DUF2) is a ubiquitous signal transduction protein domain in the Bacteria . (asm.org)
  • By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. (biomol.com)
  • DNA molecules encoding the BMP-16 proteins are also disclosed. (google.es)
  • Today, fifteen years later, the question still remains open, whether HI retroviruses actually do exist or whether the postulated retroviral HIV-antigens as well as the postulated HIV reverse transcription are a matter of human protein molecules derived from cells in co-cultured cell cultures used by both BARR -SINOUSSI et al. (whale.to)
  • He is a biochemist, and the crystals he grows at the Max Planck Institute in Tübingen are molecules from living cells, specifically proteins or ribonucleic acids (RNAs). (mpg.de)
  • Notice the potential, unused stop codon downstream of protein X. Middle, a mutation abolishes the stop codon of protein X, causing its elongation ("overprinting") to the preexisting stop codon. (asm.org)
  • The activity of ubiquitin ligase TRIM25 (tripartite motif protein 25) is necessary for RIG-I interaction with its downstream adaptor molecule, mitochondrial antiviral signaling protein (MAVS), also known as VISA, Cardif, and IPS-1 ( 6 ). (jimmunol.org)
  • Protein isolation and partial purification from chicory heads demonstrated the presence of two GAS proteins. (plantphysiol.org)
  • The reductionist approach - systematically dismantling complex systems to examine individual components - has been successful for the sciences over the past few centuries, from the isolation of the elements in chemistry, the discovery of atomic and subatomic particles in physics, to the purification and study of proteins, DNA and RNA in biology. (mpg.de)
  • Isolation of highly purified, functional carboxy-terminally truncated hepatitis B virus middle surface protein activators from eucaryotic expression systems. (uni-heidelberg.de)
  • Because it contains several domains for protein-protein interactions, including ankyrin repeats, tetratricopeptide repeats (TPRs), a coiled-coil domain, and a C-terminal PDZ (PSD-95/Dlg/ZO-1) domain-binding motif, TANC1 is thought to function as a scaffold/adaptor protein. (jneurosci.org)
  • The adaptor protein TRADD (TNFR1-associated death domain protein) is a multifunctional protein, which is an essential component of proinflammatory TNFR1 signaling ( 8 ). (jimmunol.org)
  • Quality control of protein complex assembly by a transmembrane recognition factor. (mpg.de)
  • Cf resistance proteins contain a transmembrane domain and a expected to recognize the cognate effectors peptide at the plasma membrane (PM). Interactors that could make a functional complex with Cf-9 likely belong to the family of RLKs. (wur.nl)
  • Dependent on species GH is a protein composed of approximately 190 amino acid residues corresponding to a molecular weight of approximately 22 kDa. (freepatentsonline.com)
  • α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD. (biolegend.com)
  • Because PXR is a molecular target of Rif, we hypothesized that the PXR protein is targeted by the inflammatory signaling pathway in some manner so as to compromise the ability of Rif-treated hepatocytes to mount an immunological response to infection and inflammation. (aspetjournals.org)
  • Here, we use immortalized cell lines, transgenic "humanized" PXR mice, and primary cultures of mouse and human hepatocytes to show that SUMOylation of the PXR protein represents the molecular basis of the diminished inflammatory response observed across species. (aspetjournals.org)
  • Knowledge of the identity and quantity of expressed proteins of a cell type is a prerequisite for a complete understanding of its molecular functions. (mcponline.org)
  • It is therefore important to understand the molecular processes that control platelet activation and aggregation and to develop new therapeutic strategies to block critical platelet proteins involved in these processes ( 1 ). (mcponline.org)
  • We are starting to examin auxin transport proteins with the same degree of molecular precision. (warwick.ac.uk)
  • Size exclusion chromatography revealed the presence of two oligomeric states of the full-length SUD protein: A form with a native molecular mass of 120 kD, representing a dimer or a trimer, and a form with a mass of 250 kD, potentially being a pentamer or hexamer. (egms.de)
  • Recently, the molecular composition of PPD was defined, with hundreds of mycobacterial protein representatives making up PPD. (asm.org)
  • Our results demonstrate for the first time that the PPD response can be mimicked at the molecular level with defined protein cocktails. (asm.org)
  • Insulin is a protein consisting of two peptide chains connected by three bridges in the form of molecular bonds. (princetoninfo.com)
  • Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system. (mpg.de)
  • 12 This technology is extremely efficient in dissecting intracellular protein interaction networks at the molecular level. (haematologica.org)
  • Molecular characteristics of C. elegans CED-4, an adapter protein involved in apoptosis, were studied using recombinant proteins. (tum.de)
  • With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
  • The illumination induces structural changes affecting both the carotenoid and the protein. (pnas.org)
  • Overlapping proteins have a sequence composition globally biased toward disorder-promoting amino acids and are predicted to contain significantly more structural disorder than nonoverlapping proteins. (asm.org)
  • Here, we demonstrate based on Förster resonance energy transfer (FRET) and bilayer patch-clamp studies, a direct calmodulin-independent action of calcium on the purified human TRPA1 (hTRPA1), causing structural changes and activation without immediate subsequent desensitization of hTRPA1 with and without its N-terminal ankyrin repeat domain (N-ARD). (nanion.de)
  • Finally, the two deduced amino acid sequences were modeled, and the resulting protein models were compared with the crystal structure of tobacco ( Nicotiana tabacum ) 5- epi -aristolochene synthase, which forms germacrene A as an enzyme-bound intermediate en route to 5- epi -aristolochene. (plantphysiol.org)
  • A. tumefaciens glycogen synthase (GS) purified to homogeneity from the above-mentioned cells was able to build its own primer by transferring Glc residues from ADP-Glc to an amino acid(s) in the same protein. (pnas.org)
  • The difference in the distribution of cis and trans configurations of proline residues in the protein after complete denaturation is a major determining factor of the final yield. (osti.gov)
  • A point mutation of one of four proline residues to an asparagine led to a near-doubling of the yield of refolded protein after complete denaturation. (osti.gov)
  • You will combine calculations with laboratory work using purified proteins to test the contributions of key residues in both partners using mutagenesis in order to explain selectivity. (warwick.ac.uk)
  • Geminin coiled-coil domain-containing protein 1, GemC1, is a member of the Geminin superfamily. (iucr.org)
  • In contrast to transcriptomics, proteomics approaches are intrinsically better suited for studying the cellular functions of platelets, because proteins are the biochemical functional units. (mcponline.org)
  • Although it encodes all enzymes known to be required for glycogen synthesis and utilization, the cluster does not encode a protein displaying significant similarity to glycogenin, nor does the entire A. tumefaciens genome code for such a protein ( 6 ). (pnas.org)
  • Transposons, too, need various proteins to replicate and to insert themselves into the genome. (mpg.de)
  • These proteins are necessary and sufficient for replication and transcription of the genome ( 31 ). (asm.org)
  • This precludes a clear separation of presumed retroviral and cellular proteins or extracellular matrix proteins. (whale.to)
  • Upon investigations in order to develop a vaccine against HIV it became apparent, that the enrichment of presumed HIV-1 preparations, considered as pure, consist of proteins of the cell types used in the cell cultures, which resist a clean separation into presumed retroviral and cellular proteins, i.e. extracellular matrix proteins. (whale.to)
  • Atypical HUS (aHUS) can result from genetic mutations in complement regulatory proteins including complement factors H (FH), I (FI), and B (FB), as well as other factors. (springer.com)
  • Putative effectors will be cloned in the PVX vector which will subsequently be screened tomato plants carrying the different proteins. (wur.nl)
  • Indeed, oligomerization has been observed for a number of NNSV matrix proteins, particularly for the matrix proteins of measles virus ( 38 ), Nipah virus ( 5 ), and Borna disease virus ( 26 ) as well as for the major matrix protein, VP40, of Ebola virus (EBOV) ( 44 ). (asm.org)
  • Unité de Recherche sur les Maladies Infectieuses with viral sequences identified previously in humans (BZ1 et Tropicales Emergentes, Marseilles (P.-E. Fournier, C. Robert, from feces, SL1 from cerebrospinal fluid, MSSI2.225 from C. Blanc-Tailleur, D. Raoult) blood). (cdc.gov)
  • It is widely assumed that new proteins are created by duplication, fusion, or fission of existing coding sequences. (asm.org)
  • DNA sequences simultaneously encoding two or more proteins in different reading frames, have exerted a fascination on evolutionary biologists. (asm.org)
  • To fully understand the underlying mechanisms of biological processes, it is essential to determine whether observed changes in mRNA can also be seen in the translated protein, and to pinpoint what cell types are exhibiting these changes. (biomedcentral.com)
  • Determining a direct relationship between protein and mRNA levels can be problematic, and previous efforts to find correlations have found variable success. (biomedcentral.com)
  • This technique allows tethering of a single protein for more than a day and at forces similar to those encountered in vivo. (uwm.edu)