Puma: A genus in the family FELIDAE comprising one species, Puma concolor. It is a large, long-tailed, feline of uniform color. The names puma, cougar, and mountain lion are used interchangeably for this species. There are more than 20 subspecies.Apoptosis Regulatory Proteins: A large group of proteins that control APOPTOSIS. This family of proteins includes many ONCOGENE PROTEINS as well as a wide variety of classes of INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS such as CASPASES.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Tumor Suppressor Protein p53: Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Lynx: A genus in the family FELIDAE comprising felines with long legs, ear tufts, and a short tail.bcl-2-Associated X Protein: A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein.Tumor Suppressor Proteins: Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.Proto-Oncogene Proteins c-bcl-2: Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.Carnivora: An order of MAMMALS, usually flesh eaters with appropriate dentition. Suborders include the terrestrial carnivores Fissipedia, and the aquatic carnivores PINNIPEDIA.Felidae: The cat family in the order CARNIVORA comprised of muscular, deep-chested terrestrial carnivores with a highly predatory lifestyle.bcl-2 Homologous Antagonist-Killer Protein: A multi-domain mitochondrial membrane protein and member of the bcl-2 Protein family. Bak protein interacts with TUMOR SUPPRESSOR PROTEIN P53 and promotes APOPTOSIS.Lions: Large, chiefly nocturnal mammals of the cat family FELIDAE, species Panthera leo. They are found in Africa and southern Asia.Myeloid Cell Leukemia Sequence 1 Protein: A member of the myeloid leukemia factor (MLF) protein family with multiple alternatively spliced transcript variants encoding different protein isoforms. In hematopoietic cells, it is located mainly in the nucleus, and in non-hematopoietic cells, primarily in the cytoplasm with a punctate nuclear localization. MLF1 plays a role in cell cycle differentiation.Acinonyx: A genus of long-legged, swift-moving felines (FELIDAE) from Africa (and formerly Asia) about the size of a small leopard.Cell Line, Tumor: A cell line derived from cultured tumor cells.bcl-X Protein: A member of the bcl-2 protein family that plays a role in the regulation of APOPTOSIS. Two major isoforms of the protein exist due to ALTERNATIVE SPLICING of the BCL2L1 mRNA and are referred to as Bcl-XS and Bcl-XL.Benzothiazoles: Compounds with a benzene ring fused to a thiazole ring.HCT116 Cells: Human COLORECTAL CARCINOMA cell line.Immunodeficiency Virus, Feline: A species of LENTIVIRUS, subgenus feline lentiviruses (LENTIVIRUSES, FELINE) isolated from cats with a chronic wasting syndrome, presumed to be immune deficiency. There are 3 strains: Petaluma (FIP-P), Oma (FIP-O) and Puma lentivirus (PLV). There is no antigenic relationship between FIV and HIV, nor does FIV grow in human T-cells.Cytochromes c: Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.BH3 Interacting Domain Death Agonist Protein: A member of the Bcl-2 protein family that reversibly binds MEMBRANES. It is a pro-apoptotic protein that is activated by caspase cleavage.Toluene: A widely used industrial solvent.Ascaridoidea: A superfamily of polymyarian nematode worms. An important characteristic of this group is the presence of three prominent lips around the mouth of the organism.Animals, ZooDNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.NitrophenolsMembrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Animals, Wild: Animals considered to be wild or feral or not adapted for domestic use. It does not include wild animals in zoos for which ANIMALS, ZOO is available.Gamma Rays: Penetrating, high-energy electromagnetic radiation emitted from atomic nuclei during NUCLEAR DECAY. The range of wavelengths of emitted radiation is between 0.1 - 100 pm which overlaps the shorter, more energetic hard X-RAYS wavelengths. The distinction between gamma rays and X-rays is based on their radiation source.Lentivirus Infections: Virus diseases caused by the Lentivirus genus. They are multi-organ diseases characterized by long incubation periods and persistent infection.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.ChileCaspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Feline Acquired Immunodeficiency Syndrome: Acquired defect of cellular immunity that occurs in cats infected with feline immunodeficiency virus (FIV) and in some cats infected with feline leukemia virus (FeLV).Transcription Factor CHOP: A CCAAT-enhancer binding protein that is induced by DNA DAMAGE and growth arrest. It serves as a dominant negative inhibitor of other CCAAT-enhancer binding proteins.Tunicamycin: An N-acetylglycosamine containing antiviral antibiotic obtained from Streptomyces lysosuperificus. It is also active against some bacteria and fungi, because it inhibits the glucosylation of proteins. Tunicamycin is used as tool in the study of microbial biosynthetic mechanisms.Cola: A plant genus of the family STERCULIACEAE. This is the source of the kola nut which contains CAFFEINE and is used in popular beverages.Social Media: Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.Multimedia: Materials, frequently computer applications, that combine some or all of text, sound, graphics, animation, and video into integrated packages. (Thesaurus of ERIC Descriptors, 1994)DenmarkBlogging: Using an INTERNET based personal journal which may consist of reflections, comments, and often hyperlinks.Coca: Any of several South American shrubs of the Erythroxylon genus (and family) that yield COCAINE; the leaves are chewed with alum for CNS stimulation.ShoesAlexander Disease: Rare leukoencephalopathy with infantile-onset accumulation of Rosenthal fibers in the subpial, periventricular, and subependymal zones of the brain. Rosenthal fibers are GLIAL FIBRILLARY ACIDIC PROTEIN aggregates found in ASTROCYTES. Juvenile- and adult-onset types show progressive atrophy of the lower brainstem instead. De novo mutations in the GFAP gene are associated with the disease with propensity for paternal inheritance.Commerce: The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)Orthotic Devices: Apparatus used to support, align, prevent, or correct deformities or to improve the function of movable parts of the body.Marketing: Activity involved in transfer of goods from producer to consumer or in the exchange of services.

Puma lentivirus is controlled in domestic cats after mucosal exposure in the absence of conventional indicators of immunity. (1/39)

A high percentage of free-ranging pumas (Felis concolor) are infected with feline lentiviruses (puma lentivirus, feline immunodeficiency virus Pco [FIV-Pco], referred to here as PLV) without evidence of disease. PLV establishes productive infection in domestic cats following parenteral exposure but, in contrast to domestic cat FIV, it does not cause T-cell dysregulation. Here we report that cats exposed to PLV oro-nasally became infected yet rapidly cleared peripheral blood mononuclear cell (PBMC) proviral load in the absence of a correlative specific immune response. Two groups of four specific-pathogen-free cats were exposed to PLV via the mucosal (oro-nasal) or parenteral (i.v.) route. All animals were PBMC culture positive and PCR positive within 3 weeks postinfection and seroconverted without exhibiting clinical disease; however, three or four oro-nasally infected animals cleared circulating proviral DNA within 3 months. Antibody titers reached higher levels in animals that remained persistently infected. PLV antigen-induced proliferation was slightly greater in mucosally inoculated animals, but no differences were noted in cytotoxic T-lymphocyte responses or cytokine profiles between groups. The distribution of virus was predominantly gastrointestinal as opposed to lymphoid in all animals in which virus was detected at necropsy. Possible mechanisms for viral clearance include differences in viral fitness required for crossing mucosal surfaces, a threshold dose requirement for persistence, or an undetected sterilizing host immune response. This is the first report of control of a productive feline or primate lentivirus infection in postnatally exposed, seropositive animals. Mechanisms underlying this observation will provide clues to containment of immunodeficiency disease and could prompt reexamination of vaccine-induced immunity against human immunodeficiency virus and other lentiviruses.  (+info)

Feline lentiviruses demonstrate differences in receptor repertoire and envelope structural elements. (2/39)

Feline immunodeficiency virus (FIV) causes fatal disease in domestic cats via T cell depletion-mediated immunodeficiency. Pumas and lions are hosts for apparently apathogenic lentiviruses (PLV, LLV) distinct from FIV. We compared receptor use among these viruses by: (1) evaluating target cell susceptibility; (2) measuring viral replication following exposure to specific and non-specific receptor antagonists; and (3) comparing Env sequence and structural motifs. Most isolates of LLV and PLV productively infected domestic feline T cells, but differed from domestic cat FIV by infecting cells independent of CXCR4, demonstrating equivalent or enhanced replication following heparin exposure, and demonstrating substantial divergence in amino acid sequence and secondary structure in Env receptor binding domains. PLV infection was, however, inhibited by CD134/OX40 antibody. Thus, although PLV and LLV infection interfere with FIV superinfection, we conclude that LLV and PLV utilize novel, more promiscuous mechanisms for cell entry than FIV, underlying divergent tropism and biological properties of these viruses.  (+info)

The late Miocene radiation of modern Felidae: a genetic assessment. (3/39)

Modern felid species descend from relatively recent (<11 million years ago) divergence and speciation events that produced successful predatory carnivores worldwide but that have confounded taxonomic classifications. A highly resolved molecular phylogeny with divergence dates for all living cat species, derived from autosomal, X-linked, Y-linked, and mitochondrial gene segments (22,789 base pairs) and 16 fossil calibrations define eight principal lineages produced through at least 10 intercontinental migrations facilitated by sea-level fluctuations. A ghost lineage analysis indicates that available felid fossils underestimate (i.e., unrepresented basal branch length) first occurrence by an average of 76%, revealing a low representation of felid lineages in paleontological remains. The phylogenetic performance of distinct gene classes showed that Y-chromosome segments are appreciably more informative than mitochondrial DNA, X-linked, or autosomal genes in resolving the rapid Felidae species radiation.  (+info)

A virus reveals population structure and recent demographic history of its carnivore host. (4/39)

Directly transmitted parasites often provide substantial information about the temporal and spatial characteristics of host-to-host contact. Here, we demonstrate that a fast-evolving virus (feline immunodeficiency virus, FIV) can reveal details of the contemporary population structure and recent demographic history of its natural wildlife host (Puma concolor) that were not apparent from host genetic data and would be impossible to obtain by other means. We suggest that rapidly evolving pathogens may provide a complementary tool for studying population dynamics of their hosts in "shallow" time.  (+info)

Feline lentivirus evolution in cross-species infection reveals extensive G-to-A mutation and selection on key residues in the viral polymerase. (5/39)

Factors that restrict a virus from establishing productive infection in a new host species are important to understand because cross-species transmission events are often associated with emergent viral diseases. To determine the evolutionary pressures on viruses in new host species, we evaluated the molecular evolution of a feline immunodeficiency virus derived from a wild cougar, Puma concolor, during infection of domestic cats. Analyses were based on the coding portion of genome sequences recovered at intervals over 37 weeks of infection of six cats inoculated by either intravenous or oral-nasal routes. All cats inoculated intravenously, but only one inoculated orally-nasally, became persistently viremic. There were notable accumulations of lethal errors and predominance of G-to-A alterations throughout the genome, which were marked in the viral polymerase gene, pol. Viral structural (env and gag) and accessory (vif and orfA) genes evolved neutrally or were under purifying selection. However, sites under positive selection were identified in reverse transcriptase that involved residues in the nucleotide binding pocket or those contacting the RNA-DNA duplex. The findings of extensive G-to-A alterations in this cross-species infection are consistent with the recently described editing of host cytidine deaminase on lentivirus genomes. Additionally, we demonstrate that the primary site of hypermutation is the viral pol gene and the dominant selective force acting on this feline immunodeficiency virus as it replicates in a new host species is on key residues of the virus polymerase.  (+info)

Quantification of Feline immunodeficiency virus (FIVpco) in peripheral blood mononuclear cells, lymph nodes and plasma of naturally infected cougars. (6/39)

Infection of domestic cats with Feline immunodeficiency virus (FIV) results in a fatal immunodeficiency disease, similar to Human immunodeficiency virus 1 (HIV-1) in humans. Elevated plasma viral loads in domestic cats are correlated to decreased survival time and disease progression. However, FIV is also maintained as an apathogenic infection in other members of the family Felidae including cougars, Puma concolour (FIVpco). It is not known whether the lack of disease in cougars is a result of diminished virus replication. A real-time PCR assay was developed to quantify both FIVpco proviral and plasma viral loads in naturally infected cougars. Proviral loads quantified from peripheral blood mononuclear cells (PBMC) ranged from 2.90 x 10(1) to 6.72 x 10(4) copies per 10(6) cells. Plasma viral loads ranged from 2.30 x 10(3) to 2.81 x 10(6) RNA copies ml(-1). These data indicate that FIVpco viral loads are comparable to viral loads observed in endemic and epidemic lentivirus infections. Thus, the lack of disease in cougars is not due to low levels of virus replication. Moreover, significant differences observed among cougar PBMC proviral loads correlated to viral lineage and cougar age (P=0.014), which suggests that separate life strategies exist within FIVpco lineages. This is the first study to demonstrate that an interaction of lentivirus lineage and host age significantly effect proviral loads.  (+info)

Listeric meningoencephalomyelitis in a cougar (Felis concolor): characterization by histopathologic, immunohistochemical, and molecular methods. (7/39)

Listeria monocytogenes has been recognized as an important food-borne pathogen in animals. Records of the disease caused by this bacterium in large felids are, however, rare. The nervous form of listeriosis was diagnosed in a 12-year-old male cougar (Felis concolor) with a several-day history of neurologic disease characterized by excess salivation, head pressing, and circling that progressed to recumbency and death. Microscopically, the main alteration in the brain and spinal cord was a variably severe meningoencephalomyelitis composed mainly of mononuclear cell aggregates with fewer neutrophils. L. monocytogenes was isolated from the brain by microbiological culture, and L. monocytogenes antigen was detected in formalin-fixed, paraffin-embedded sections of brain and spinal cord by immunohistochemical analysis. On the basis of the nucleotide sequence of the 16S rRNA gene, the isolated strain was determined to be serotype 1/2a. Food-borne transmission of the bacterium was suspected, but food was not available for testing.  (+info)

Stochastic predation events and population persistence in bighorn sheep. (8/39)

Many studies have reported temporal changes in the relative importance of density-dependence and environmental stochasticity in affecting population growth rates, but they typically assume that the predominant factor limiting growth remains constant over long periods of time. Stochastic switches in limiting factors that persist for multiple time-steps have received little attention, but most wild populations may periodically experience such switches. Here, we consider the dynamics of three populations of individually marked bighorn sheep (Ovis canadensis) monitored for 24-28 years. Each population experienced one or two distinct cougar (Puma concolor) predation events leading to population declines. The onset and duration of predation events were stochastic and consistent with predation by specialist individuals. A realistic Markov chain model confirms that predation by specialist cougars can cause extinction of isolated populations. We suggest that such processes may be common. In such cases, predator-prey equilibria may only occur at large geographical and temporal scales, and are unlikely with increasing habitat fragmentation.  (+info)

  • They collected more than 24,000 individual beetles belonging to 215 different species from the bodies of puma prey-greater numbers and diversity than found in surrounding areas. (atlasobscura.com)
  • The results show that pumas as more than predators: They're "ecosystem engineers," researchers write in the study , published in the journal Oecologia . (atlasobscura.com)
  • Yet pumas-also known as mountain lions-are extraordinarily strong and crafty, and can take down large game, such as elk and mule deer, that they can't possibly eat on their own. (atlasobscura.com)
  • Most of the focus around mountain lions is typically related to state management objectives," says Josh Barry, a graduate researcher with Panthera , a global wildcat conservation organization that conducted a study, led by puma researcher Mark Elbroch, examining the impact of puma hunting habits. (atlasobscura.com)
  • Named P1 (Puma 1) for our scientific records, the mountain lion is estimated to be approximately 8-10 years old, as indicated by her dental condition. (egret.org)
  • In previous studies, Elbroch had already documented dozens of large vertebrates-wolves, foxes, black and grizzly bears-that feed on carrion left by pumas. (atlasobscura.com)
  • We have identified a novel gene named PUMA (p53 upregulated modulator of apoptosis) as a target for activation by p53. (nih.gov)
  • In this issue of Circulation , Mandl et al 4 present convincing evidence for a novel mechanism contributing to the progression of heart failure through a proapoptotic pathway mediated by p53-upregulated modulator of apoptosis (Puma), a BH3-only member of the Bcl-2 family, which induces apoptosis mediated partly via the tumor suppressor gene p53 pathway. (ahajournals.org)
  • report that low-level expression of a protein called PUMA (p53-upregulated modulator of apoptosis) distinguishes stem-like cells in cancer patients who experienced tumor recurrence during a 10-year follow-up period. (eurekalert.org)
  • A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. (thermofisher.com)
  • Here, we identified rare stem-like cells in patients' tumors characterized by low levels of the proapoptotic molecule p53-upregulated modulator of apoptosis (PUMA) and showed that these cells play a critical role in tumor progression that is independent of clinical subtype. (jci.org)
  • Puma Biotechnology, Inc. , a biopharmaceutical company, announced publication of abstracts on neratinib for the American Association for Cancer Research Annual Meeting 2019. (yahoo.com)
  • Here's what you need to know about neratinib and why Puma investors got nervous. (fool.com)
  • Puma is essentially a one-drug company, which means its fate is tied to neratinib, a drug it licensed from Pfizer in 2011 when Pfizer was downsizing to blunt the patent loss of its top-selling cholesterol drug Lipitor. (fool.com)
  • A year ago, Puma kicked off a 600-patient phase 3 trial that pitted oral neratinib and Roche 's ( NASDAQOTH:RHHBY ) Xeloda head to head against GlaxoSmithKline 's Tykerb and Xeloda. (fool.com)
  • Since nearly 40% of neratinib patients suffered from diarrhea, Puma has adjusted its treatment to include an anti-diarrhea medicine that appears to effectively reduce occurrences to manageable levels. (fool.com)
  • In March 2016, the FDA recommended Puma delay its approval application for neratinib due to design changes made to the neratinib study, but the company went ahead with the application anyway. (benzinga.com)
  • Puma in-licenses the global development and commercialization rights to three drug candidates - PB272 (neratinib, oral), PB272 (neratinib, intravenous) and PB357. (biospace.com)
  • Shares in Puma Biotechnology have rocketed after its investigational breast cancer drug neratinib, licensed from Pfizer, has hit its primary endpoint in a late-stage trial. (pharmatimes.com)
  • Shares of Puma Biotechnology are up almost 300% after the company posted strong top-line results for its flagship cancer drug neratinib. (fool.com)
  • After soaring by over 350% last year , Puma has fallen hard in recent months due to a mysterious case of investor pessimism for its cancer drug PB272, aka neratinib. (fool.com)
  • Specifically, Puma shares cratered by 25% in a single day after the company earlier this year announced partial midstage trial results for neratinib as a potential treatment for breast cancer that has spread to the brain. (fool.com)
  • Pfizer this week amended its licensing agreement with Puma for neratinib, pushing the bulk of clinical trial expenses onto Puma and giving up a nice chunk of potential royalty payments in the process. (fool.com)
  • Per the new agreement, Puma is now responsible for funding the ongoing legacy trials for neratinib, which will substantially increase the company's expenses moving forward. (fool.com)
  • Almost immediately after announcing this amended agreement, Puma on Tuesday released top-line data from a late-stage study for neratinib as a treatment for HER2-positive breast cancer. (fool.com)
  • The awards were granted as an inducement material to the new employees entering into employment with Puma, in accordance with Nasdaq Listing Rule 5635(c)(4). (yahoo.com)
  • November 30, 2018 - A female puma stalked a male guanaco for more than an hour waiting for the right moment to pounce. (nationalgeographic.com)
  • Chicago - March 28, 2005 - PUMA North America Inc., a leading producer of athletic footwear, apparel and accessories, said it is using SPSS predictive analytics software to manage its inventory real-time across its domestic retail operations. (sdcexec.com)
  • With SPSS, PUMA has completely replaced 80 percent of its [enterprise resource planning (ERP)] system reports, which were highly inflexible and required us to contact the vendor for simple template changes," said Karen King, a database analyst for PUMA North America. (sdcexec.com)
  • Most research has been conducted on pumas in North America, where adult males such as this one-likely the mate of the female and the father of the two kittens-don't hang around, and especially don't dine, with others. (outdoorphotographer.com)
  • Puma Biotechnology Inc. shares surged 49.2% to $56.30 in morning trade Monday after the Food and Drug Administration released favorable briefing documents for an advisory committee meeting this week that will review Puma Biotech's breast cancer drug. (foxbusiness.com)
  • Puma announced that it has amended its licensing deal with Pfizer, originally signed in October 2011, which makes the Los Angeles-based biotech responsible for clinical trial expenses, though it will pay lower royalties. (pharmatimes.com)
  • Rare PUMA Clyde Snake Sneakers - New without Box. (ebay.com)
  • To find out, Puma is tracking environmental costs inflicted at every step of the manufacturing process that transforms rubber, cotton and leather into sneakers. (manufacturing.net)
  • It is rare for pumas to feed on carcasses that they did not kill. (britannica.com)
  • The researchers studied a total of 18 elk and mule deer carcasses killed by pumas in Bridger-Teton National Forest in Wyoming, returning weekly over the course of six months from May to October 2016 to check beetle traps and observe how populations shifted over time. (atlasobscura.com)
  • The feline in question is Puma concolor , our nation's largest cat species, unless you count the odd wayward jaguar. (psmag.com)
  • It's the complete opposite of what we've been saying about pumas and solitary species for over 60 years," said lead author and Panthera Puma Program Lead Scientist Mark Elbroch, Ph.D. "We were shocked -- this research allows us to break down mythologies and question what we thought we knew. (eurekalert.org)
  • Then, they used cutting-edge analyses of puma networks to reveal that the species exhibits social strategies like more social animals, just over longer timescales. (eurekalert.org)
  • Additionally, adolescent male brothers of some species, including cheetahs, lions and pumas often stick together after dispersing, cooperating to obtain food and hanging out together. (thecatcoach.com)
  • Our HM74A/PUMA-G/GPR109A/NIACR1 Antibodies can be used in a variety of model species: Human, Rat. (novusbio.com)
  • Puma Pacer Next Excel Sneaker - Black US 12, Men's Pumablackpumawhite Pieced mesh lace-up sneaker SOFTFOAM+ optimal comfort insert. (pricegrabber.com)
  • Puma Biotechnology, Inc. and Pierre Fabre have entered into an exclusive license agreement under which Pierre Fabre will develop and commercialize NERLYNX® within Europe and part of Africa. (yahoo.com)
  • NERLYNX is a registered trademark of Puma Biotechnology, Inc. (marketwatch.com)
  • Health Canada's acceptance of our NDS represents another important regulatory milestone in our commitment to increasing access to NERLYNX around the world to reduce disease recurrence following trastuzumab therapy in patients with early stage HER2-positive breast cancer," said Alan H. Auerbach, Chief Executive Officer and President of Puma. (biospace.com)
  • This antibody is specific for both the alpha and beta isoforms of PUMA. (abcam.com)
  • The following antibody was used in this experiment: PUMA Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA1-4277, RRID AB_2064549. (thermofisher.com)
  • By Western blot this antibody recognizes both forms of the PUMA protein found at ~20 kDa and ~36 kDa in transfected U20S cells. (thermofisher.com)
  • Each HM74A/PUMA-G/GPR109A/NIACR1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Choose from our HM74A/PUMA-G/GPR109A/NIACR1 polyclonal antibodies and browse our HM74A/PUMA-G/GPR109A/NIACR1 monoclonal antibody catalog. (novusbio.com)
  • A male elk can weigh in at 700 pounds or more-over three times the size of a male puma. (atlasobscura.com)
  • She has just spotted a large male puma making his way down the hill to the guanaco kill we've been watching for the last hour. (outdoorphotographer.com)
  • A 12-year-old adult, intact male puma ( Puma concolor araucana ) was evaluated because of abnormal hind limb gait and paresis. (scielo.cl)
  • A 12 year old, 53-kg intact male puma ( Puma concolor araucana ) was presented to the Veterinary Teaching Hospital at Universidad Austral de Chile, Valdivia, Chile, because of pelvic limb paraparesis and walking difficulty of two weeks duration. (scielo.cl)
  • The mobile Storefinder gives users the functionality to search for nearby PUMA stores based on their location and relies on a simple user interface to help users easily find key information about PUMA stores. (viget.com)
  • Find top designer fashion products for your puma black shoes search on ShopStyle. (shopstyle.com)
  • This page displays 748 of the most similar products that matched your search, including top products from Alexander McQueen, Balmain and Puma, & from top retailers such as Amazon.com, Farfetch and Nordstrom, all in one place. (shopstyle.com)
  • The Pumas have never beaten New Zealand in 24 attempts, typically pushing the All Blacks for the first hour before the world champions kick away. (reuters.com)
  • PUMA today revealed Tricks, a new colourful interpretation of its evoPOWER and evoSPEED football boots, made for the unbelievable at the 2014 FIFA World CupTM in Brazil. (mynewsdesk.com)
  • Herzogenaurach, Germany (14 March, 2016) - Sports brand PUMA unveiled today its latest women's training campaign focusing on their new fitness footwear style offering, the PUMA Fierce. (mynewsdesk.com)
  • Global Sports Brand PUMA has released a Sportstyle capsule collection for Autumn-Winter '15 for men and women that features classic apparel pieces inspired by its sports heritage with modern twists. (thenewsmarket.com)
  • Most of the focus around mountain lions is typically related to state management objectives," says Josh Barry, a graduate researcher with Panthera , a global wildcat conservation organization that conducted a study, led by puma researcher Mark Elbroch, examining the impact of puma hunting habits. (atlasobscura.com)
  • His dedication and patience allowed him to photograph pumas mating, hunting guanaco (a llama relative), and engaging in other behaviors that few people have ever seen. (nationalgeographic.com)
  • Entrepreneur and style icon, Kylie Jenner headlines the campaign along with other PUMA ambassadors which launches April 1, 2016. (mynewsdesk.com)
  • After all, it is hard to write about the Super Puma, particularly the H225, without mentioning a 2016 crash in Norway in which all 13 passengers and crew on board were killed. (flightglobal.com)
  • April 14, 2016 - IMPERX introduces PUMA 2MP day/night camera that can capture 1080p (1920 x 1080) video in scenes with widely varying lighting conditions, from sunlight to starlight and from highlights to shadows. (automation.com)
  • Björn Gulden, Chief Executive Officer for PUMA said: "Extending this long standing partnership with the FIGC is extremely important to PUMA and is another key step towards our goal to become the Fastest Sport Brand in the World. (mynewsdesk.com)
  • O nome puma , que tomou Jardine para denominar o xénero, comezou a empegarse nas linguas europeas en 1777, e deriva do castelán puma , que o tomou do quechua dos incas puma . (wikipedia.org)
  • 2,0 2,1 Puma Jardine, 1834 no ITIS . (wikipedia.org)
  • Designed exclusively for Arsenal, the new left-footed PUMA evoPOWER football was created to increase the accuracy, power and precision of the often disadvantaged, left footed players. (mynewsdesk.com)
  • Cesc Fabregas, Yaya Toure and Mario Balotelli are some of the stars that will be donning the new Puma evoPOWER 1.2 boots in the coming weeks. (mirror.co.uk)
  • By 12 weeks after surgery, Puma −/− mice showed a decline in fractional shortening, suggesting that Puma knockdown delayed, but did not fully prevent, pressure-overload cardiac dysfunction. (ahajournals.org)
  • E) Autophagy inhibition by shRNA knockdown of ATG5, ATG7, ATG12, Vps34 or p62/SQSTM1 all cause an increase in PUMA levels. (nih.gov)
  • F) PUMA knockdown is sufficient to delay MOMP in response to TRAIL treatment. (nih.gov)
  • PUMA knockdown is sufficient delay MOMP similarly to trehalose. (nih.gov)
  • Rihanna has taken a fun and cozy spin to a classic PUMA sport style to create the Fur Slides, the third new footwear style under her FENTY label. (mynewsdesk.com)
  • Rihanna's uncle is reportedly facing charges of selling fake copies of items from her Puma x Fenty line. (hollywood.com)
  • Rihanna , 29, real name Robyn Rihanna Fenty , unveiled her Fenty x Puma fashion line last year (16) at a star-studded event during New York Fashion Week . (hollywood.com)
  • better source needed] Injured livestock of 1964, police-taken paw cast and naming of 'the Surrey Puma' In August 1964, a bullock at Bushylease Farm was found severely lacerated. (wikipedia.org)
  • That's why we've developed an efficient, multi-purpose tractor line with just the right amount of power for both row-crop and livestock applications - the Puma™ Series Tractors. (caseih.com)
  • SPAIN'S NEWS) The mark of Puma shoes it has become a trend on Twitter after several users in Russia detected the amazing resemblance between one of his shoe models and the face of Adolf Hitler. (wnd.com)
  • Boys - Puma Enzo Jr. Shoe - Black 5 Pumablack Style 19018902/Sku 949362 Pieced. (pricegrabber.com)
  • The Spring/Summer shoe collection from Puma features new models for both men and women, and include many of Puma's unique technologies including their Disc closure system, Ignite foam, and Epic Performance last. (golf.com)
  • The new Puma Ignite PwrSport golf shoe. (golf.com)
  • The awards were granted under Puma's 2017 Employment Inducement Incentive Award Plan, which was adopted on April 27, 2017 and provides for the granting of equity awards to new employees of Puma. (yahoo.com)
  • The PUMA CELL Venom is running hot in a blistering 'Ribbon Red' colourway, the 1998 running silhouette enjoying a revival in 2019. (sneakerfreaker.com)
  • Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway. (nih.gov)
  • Taken together, these findings point to PUMA as a key vulnerability of stem-like cells and suggest that pharmacological upregulation of PUMA via Src inhibition may represent a strategy to selectively target these cells in a wide spectrum of aggressive breast cancers. (jci.org)
  • The Suede Heart by Puma features the same iconic silhouette as the `Suede Classic`, with the addition of wide eyelets and ribbon laces. (houseoffraser.co.uk)
  • Florence, Italy (September 2015) - The Italian Football Association (FIGC) and PUMA has revealed the new away kit for all national men's, women's and youth teams. (mynewsdesk.com)
  • Clean the outer surface of your canvas, imitation leather, leather or nylon Pumas using a clean cloth that has been dampened with water and mild soap (shampoo, liquid hand soap or dishwashing soap). (ehow.com)
  • Puma Biotechnology Inc. said its investigational breast-cancer treatment showed a 33% improvement in disease-free survival over a placebo in a phase III study. (wsj.com)
  • Although neratinib's exact role in the clinic for early stage HER2-positive breast cancer patients remains to be seen, the drug's path toward blockbuster status certainly became a lot easier after Puma posted these strong trial results. (fool.com)
  • Puma shoes are designed to be sturdy and long-lasting shoes. (ehow.com)
  • With minimal care, Puma shoes can look new for a long time. (ehow.com)
  • Do not wash Puma shoes in a washing machine, and do not dry them in a clothes dryer. (ehow.com)
  • Be sure to check out Puma Ferrari Black Shoes and Puma Shoes. (shopstyle.com)
  • Shopping for the best deal on Baby puma crib shoes Baby & Kids' Shoes - Select a product below or see other matches to read product reviews, compare prices and store ratings. (pricegrabber.com)
  • We unbox the newest pair of spikeless golf shoes from Puma, the Ignite Disc Extreme, of which a portion of sales benefits Folds of Honor. (golf.com)
  • The new Puma Grip Fusion golf shoes. (golf.com)
  • The new Puma Monolite Cat Woven golf shoes. (golf.com)
  • The new Puma Ignite Statement Low golf shoes. (golf.com)
  • While Puma hasn't been able to break out the environmental cost of manufacturing a pair of shoes or other individual products, the label in 2010 put the estimated cost of its overall environmental impact at 145 million euros ($185 million). (manufacturing.net)
  • For best results, clean your Pumas as soon as they become dirty. (ehow.com)
  • After all, investors awarded Puma an eye-popping $3 billion market cap based solely on mid-stage results for one drug. (fool.com)
  • Puma Exploration (PUM-TSXV) recently announced the results of a recent ground geophysical survey carried out on the Turgeon Cu-Zn VMS project in northern New Brunswick. (thebullandbear.com)
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