A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
Infections with bacteria of the genus PSEUDOMONAS.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.
Proteins found in any species of bacterium.
A species of nonpathogenic fluorescent bacteria found in feces, sewage, soil, and water, and which liquefy gelatin.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
Substances that reduce the growth or reproduction of BACTERIA.
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
Antibiotic pigment produced by Pseudomonas aeruginosa.
Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.
Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
An aminoglycoside, broad-spectrum antibiotic produced by Streptomyces tenebrarius. It is effective against gram-negative bacteria, especially the PSEUDOMONAS species. It is a 10% component of the antibiotic complex, NEBRAMYCIN, produced by the same species.
The functional hereditary units of BACTERIA.
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
Bacteriocins elaborated by mutant strains of Pseudomonas aeruginosa. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same or related species.
A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.
Broad-spectrum semisynthetic penicillin derivative used parenterally. It is susceptible to gastric juice and penicillinase and may damage platelet function.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Toxins produced, especially by bacterial or fungal cells, and released into the culture medium or environment.
Semisynthetic, broad-spectrum antibacterial derived from CEPHALORIDINE and used especially for Pseudomonas and other gram-negative infections in debilitated patients.
Semisynthetic thienamycin that has a wide spectrum of antibacterial activity against gram-negative and gram-positive aerobic and anaerobic bacteria, including many multiresistant strains. It is stable to beta-lactamases. Clinical studies have demonstrated high efficacy in the treatment of infections of various body systems. Its effectiveness is enhanced when it is administered in combination with CILASTATIN, a renal dipeptidase inhibitor.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.
A species of gram-negative, fluorescent, phytopathogenic bacteria in the genus PSEUDOMONAS. It is differentiated into approximately 50 pathovars with different plant pathogenicities and host specificities.
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.
A complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.
Infections in the inner or external eye caused by microorganisms belonging to several families of bacteria. Some of the more common genera found are Haemophilus, Neisseria, Staphylococcus, Streptococcus, and Chlamydia.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Proteins isolated from the outer membrane of Gram-negative bacteria.
Beta-lactam antibiotics that differ from PENICILLINS in having the thiazolidine sulfur atom replaced by carbon, the sulfur then becoming the first atom in the side chain. They are unstable chemically, but have a very broad antibacterial spectrum. Thienamycin and its more stable derivatives are proposed for use in combinations with enzyme inhibitors.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Vaccines or candidate vaccines used to prevent or treat PSEUDOMONAS INFECTIONS.
A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC 3.4.21.36.
Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.
A bacterial protein from Pseudomonas, Bordetella, or Alcaligenes which operates as an electron transfer unit associated with the cytochrome chain. The protein has a molecular weight of approximately 16,000, contains a single copper atom, is intensively blue, and has a fluorescence emission band centered at 308nm.
A broad-spectrum antimicrobial carboxyfluoroquinoline.
The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
A species of gram-negative bacteria in the genus PSEUDOMONAS, containing multiple genomovars. It is distinguishable from other pseudomonad species by its ability to use MALTOSE and STARCH as sole carbon and energy sources. It can degrade ENVIRONMENTAL POLLUTANTS and has been used as a model organism to study denitrification.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
A broad-spectrum antibiotic derived from KANAMYCIN. It is reno- and oto-toxic like the other aminoglycoside antibiotics.
Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.
A semisynthetic ampicillin-derived acylureido penicillin.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Loss of epithelial tissue from the surface of the cornea due to progressive erosion and necrosis of the tissue; usually caused by bacterial, fungal, or viral infection.
A monocyclic beta-lactam antibiotic originally isolated from Chromobacterium violaceum. It is resistant to beta-lactamases and is used in gram-negative infections, especially of the meninges, bladder, and kidneys. It may cause a superinfection with gram-positive organisms.
Inflammation of the cornea.
Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Inflammation of the lung parenchyma that is caused by bacterial infections.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A group of broad-spectrum antibiotics first isolated from the Mediterranean fungus ACREMONIUM. They contain the beta-lactam moiety thia-azabicyclo-octenecarboxylic acid also called 7-aminocephalosporanic acid.
Basic lipopeptide antibiotic group obtained from Bacillus polymyxa. They affect the cell membrane by detergent action and may cause neuromuscular and kidney damage. At least eleven different members of the polymyxin group have been identified, each designated by a letter.
Any infection which a patient contracts in a health-care institution.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Semisynthetic, broad-spectrum, AMPICILLIN derived ureidopenicillin antibiotic proposed for PSEUDOMONAS infections. It is also used in combination with other antibiotics.
Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)
Hydrogen cyanide (HCN); A toxic liquid or colorless gas. It is found in the smoke of various tobacco products and released by combustion of nitrogen-containing organic materials.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.
Any normal or abnormal coloring matter in PLANTS; ANIMALS or micro-organisms.
Bacteria which retain the crystal violet stain when treated by Gram's method.
Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).
Invasion of the site of trauma by pathogenic microorganisms.
Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).
Polysaccharides found in bacteria and in capsules thereof.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Antibiotic complex produced by Streptomyces kanamyceticus from Japanese soil. Comprises 3 components: kanamycin A, the major component, and kanamycins B and C, the minor components.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Injuries to tissues caused by contact with heat, steam, chemicals (BURNS, CHEMICAL), electricity (BURNS, ELECTRIC), or the like.
Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
Four-membered cyclic AMIDES, best known for the PENICILLINS based on a bicyclo-thiazolidine, as well as the CEPHALOSPORINS based on a bicyclo-thiazine, and including monocyclic MONOBACTAMS. The BETA-LACTAMASES hydrolyze the beta lactam ring, accounting for BETA-LACTAM RESISTANCE of infective bacteria.
An antibiotic derived from penicillin similar to CARBENICILLIN in action.
A group of derivatives of naphthyridine carboxylic acid, quinoline carboxylic acid, or NALIDIXIC ACID.
Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.
Infections by bacteria, general or unspecified.
Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.
A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.
A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.
Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.
Viruses whose hosts are bacterial cells.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
Nonsusceptibility of bacteria to the action of the beta-lactam antibiotics. Mechanisms responsible for beta-lactam resistance may be degradation of antibiotics by BETA-LACTAMASES, failure of antibiotics to penetrate, or low-affinity binding of antibiotics to targets.
Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A building block of penicillin, devoid of significant antibacterial activity. (From Merck Index, 11th ed)
Techniques used in studying bacteria.
A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.
Cyclic AMIDES formed from aminocarboxylic acids by the elimination of water. Lactims are the enol forms of lactams.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
The rate dynamics in chemical or physical systems.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
A sub-class of PEPTIDE HYDROLASES that act only near the ends of polypeptide chains.
The presence of viable bacteria circulating in the blood. Fever, chills, tachycardia, and tachypnea are common acute manifestations of bacteremia. The majority of cases are seen in already hospitalized patients, most of whom have underlying diseases or procedures which render their bloodstreams susceptible to invasion.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
A group of enzymes that catalyze an intramolecular transfer of a phosphate group. It has been shown in some cases that the enzyme has a functional phosphate group, which can act as the donor. These were previously listed under PHOSPHOTRANSFERASES (EC 2.7.-). (From Enzyme Nomenclature, 1992) EC 5.4.2.
The generic name for the group of aliphatic hydrocarbons Cn-H2n+2. They are denoted by the suffix -ane. (Grant & Hackh's Chemical Dictionary, 5th ed)
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A synthetic fluoroquinolone (FLUOROQUINOLONES) with broad-spectrum antibacterial activity against most gram-negative and gram-positive bacteria. Norfloxacin inhibits bacterial DNA GYRASE.
A natural association between organisms that is detrimental to at least one of them. This often refers to the production of chemicals by one microorganism that is harmful to another.
A group of QUINOLONES with at least one fluorine atom and a piperazinyl group.
The presence of an infectious agent on instruments, prostheses, or other inanimate articles.
A pyridinium-substituted semisynthetic, broad-spectrum antibacterial used especially for Pseudomonas infections in debilitated patients.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
The genetic complement of a BACTERIA as represented in its DNA.
Pathological processes involving any part of the LUNG.
DNA elements that include the component genes and insertion site for a site-specific recombination system that enables them to capture mobile gene cassettes.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A synthetic fluoroquinolone antibacterial agent that inhibits the supercoiling activity of bacterial DNA GYRASE, halting DNA REPLICATION.
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans.
Benzoic acid or benzoic acid esters substituted with one or more chlorine atoms.
Inflammation of the lung parenchyma that is associated with BRONCHITIS, usually involving lobular areas from TERMINAL BRONCHIOLES to the PULMONARY ALVEOLI. The affected areas become filled with exudate that forms consolidated patches.
An enzyme that catalyzes the reversible isomerization of D-mannose-6-phosphate to form D-fructose-6-phosphate, an important step in glycolysis. EC 5.3.1.8.
The action of a drug in promoting or enhancing the effectiveness of another drug.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Persistent abnormal dilatation of the bronchi.
Substances used on inanimate objects that destroy harmful microorganisms or inhibit their activity. Disinfectants are classed as complete, destroying SPORES as well as vegetative forms of microorganisms, or incomplete, destroying only vegetative forms of the organisms. They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.
Monocyclic, bacterially produced or semisynthetic beta-lactam antibiotics. They lack the double ring construction of the traditional beta-lactam antibiotics and can be easily synthesized.
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
A species of gram-negative bacteria in the genus PSEUDOMONAS, which is found in SOIL and WATER.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. This enzyme was formerly characterized as EC 1.13.1.2.
A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
The space between the inner and outer membranes of a cell that is shared with the cell wall.
The sum of the weight of all the atoms in a molecule.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Peptides composed of between two and twelve amino acids.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
Semisynthetic broad-spectrum cephalosporin with a tetrazolyl moiety that is resistant to beta-lactamase. It has been proposed especially against Pseudomonas infections.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A group of enzymes that oxidize diverse nitrogenous substances to yield nitrite. (Enzyme Nomenclature, 1992) EC 1.
Nonsusceptibility of an organism to the action of penicillins.
Measurable quantity of bacteria in an object, organism, or organism compartment.
QUINOLONES containing a 4-oxo (a carbonyl in the para position to the nitrogen). They inhibit the A subunit of DNA GYRASE and are used as antimicrobials. Second generation 4-quinolones are also substituted with a 1-piperazinyl group at the 7-position and a fluorine at the 6-position.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria occurring in soil and water. Its organisms are generally nonpathogenic, but some species do cause infections of mammals, including humans.
Inflammation of the OUTER EAR including the external EAR CANAL, cartilages of the auricle (EAR CARTILAGE), and the TYMPANIC MEMBRANE.
A group of 1,2-benzenediols that contain the general formula R-C6H5O2.
Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.
Diseases of plants.
Life or metabolic reactions occurring in an environment containing oxygen.
The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST.
Inflammation of follicles, primarily hair follicles.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Community of tiny aquatic PLANTS and ANIMALS, and photosynthetic BACTERIA, that are either free-floating or suspended in the water, with little or no power of locomotion. They are divided into PHYTOPLANKTON and ZOOPLANKTON.
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A widely used industrial solvent.
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
Simultaneous resistance to several structurally and functionally distinct drugs.

UK-18892, a new aminoglycoside: an in vitro study. (1/9595)

UK-18892 is a new aminoglycoside antibiotic, a derivative of kanamycin A structurally related to amikacin. It was found to be active against a wide range of pathogenic bacteria, including many gentamicin-resistant strains. The spectrum and degree of activity of UK-18892 were similar to those of amikacin, and differences were relatively minor. UK-18892 was about twice as active as amikacin against gentamicin-susceptible strains of Pseudomonas aeruginosa. Both amikacin and UK-18892 were equally active against gentamicin-resistant strains of P. aeruginosa. There were no appreciable differences in the activity of UK-18892 and amikacin against Enterobacteriaceae and Staphylococcus aureus. Cross-resistance between these two antimicrobials was also apparent.  (+info)

Modified peptidoglycan transpeptidase activity in a carbenicillin-resistant mutant of Pseudomonas aeruginosa 18s. (2/9595)

A carbenicillin-resistant mutant of Pseudomonas aeruginosa 18s was found to possess peptidoglycan transpeptidase activity significantly more resistant to inhibition by benzyl penicillin, ampicillin, carbenicillin, and cephaloridine than that of the parent strain. The mutant was more resistant than the parent strain to all of the beta-lactam antibiotics tested, and 50% inhibition values for these compounds against membrane-bound model transpeptidase activity paralleled this increase. The resistance of the mutant to kanamycin, streptomycin, and chloramphenicol was unchanged.  (+info)

Improved medium for recovery and enumeration of Pseudomonas aeruginosa from water using membrane filters. (3/9595)

A modified mPA medium, designated mPA-C, was shown to recover Pseudomonas aeruginosa from a variety of water sources with results comparable to those with mPA-B and within the confidence limits of a most-probable-number technique. Enumeration of P. aeruginosa on mPA-C was possible after only 24 h of incubation at 41.5 degrees C, compared with 72 h of incubation required for mPA-B and 96 h of incubation for a presumptive most probable number.  (+info)

Automated food microbiology: potential for the hydrophobic grid-membrane filter. (4/9595)

Bacterial counts obtained on hydrophobic grid-membrane filters were comparable to conventional plate counts for Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus in homogenates from a range of foods. The wide numerical operating range of the hydrophobic grid-membrane filters allowed sequential diluting to be reduced or even eliminated, making them attractive as components in automated systems of analysis. Food debris could be rinsed completely from the unincubated hydrophobic grid-membrane filter surface without affecting the subsequent count, thus eliminating the possibility of counting food particles, a common source of error in electronic counting systems.  (+info)

Denitrifying Pseudomonas aeruginosa: some parameters of growth and active transport. (5/9595)

Optimal cell yield of Pseudomonas aeruginosa grown under denitrifying conditions was obtained with 100 mM nitrate as the terminal electron acceptor, irrespective of the medium used. Nitrite as the terminal electron acceptor supported poor denitrifying growth when concentrations of less than 15 mM, but not higher, were used, apparently owing to toxicity exerted by nitrite. Nitrite accumulated in the medium during early exponential phase when nitrate was the terminal electron acceptor and then decreased to extinction before midexponential phase. The maximal rate of glucose and gluconate transport was supported by 1 mM nitrate or nitrite as the terminal electron acceptor under anaerobic conditions. The transport rate was greater with nitrate than with nitrite as the terminal electron acceptor, but the greatest transport rate was observed under aerobic conditions with oxygen as the terminal electron acceptor. When P. aeruginosa was inoculated into a denitrifying environment, nitrate reductase was detected after 3 h of incubation, nitrite reductase was detected after another 4 h of incubation, and maximal nitrate and nitrite reductase activities peaked together during midexponential phase. The latter coincided with maximal glucose transport activity.  (+info)

The Pseudomonas aeruginosa secretory product pyocyanin inactivates alpha1 protease inhibitor: implications for the pathogenesis of cystic fibrosis lung disease. (6/9595)

Alpha1 Protease inhibitor (alpha1PI) modulates serine protease activity in the lung. Reactive oxygen species inactivate alpha1PI, and this process has been implicated in the pathogenesis of a variety of forms of lung injury. An imbalance of protease-antiprotease activity is also detected in the airways of patients with cystic fibrosis-associated lung disease who are infected with Pseudomonas aeruginosa. P. aeruginosa secretes pyocyanin, which, through its ability to redox cycle, induces cells to generate reactive oxygen species. We tested the hypothesis that redox cycling of pyocyanin could lead to inactivation of alpha1PI. When alpha1PI was exposed to NADH and pyocyanin, a combination that results in superoxide production, alpha1PI lost its ability to form an inhibitory complex with both porcine pancreatic elastase (PPE) and trypsin. Similarly, addition of pyocyanin to cultures of human airway epithelial cells to which alpha1PI was also added resulted in a loss of the ability of alpha1PI to form a complex with PPE or trypsin. Neither superoxide dismutase, catalase, nor dimethylthiourea nor depletion of the media of O2 to prevent formation of reactive oxygen species blocked pyocyanin-mediated inactivation of alpha1PI. These data raise the possibility that a direct interaction between reduced pyocyanin and alpha1PI is involved in the process. Consistent with this possibility, pretreatment of alpha1PI with the reducing agent beta-mercaptoethanol also inhibited binding of trypsin to alpha1PI. These data suggest that pyocyanin could contribute to lung injury in the P. aeruginosa-infected airway of cystic fibrosis patients by decreasing the ability of alpha1PI to control the local activity of serine proteases.  (+info)

Safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein F-I vaccine in human volunteers. (7/9595)

A hybrid protein [Met-Ala-(His)6OprF190-342-OprI21-83] consisting of the mature outer membrane protein I (OprI) and amino acids 190 to 342 of OprF of Pseudomonas aeruginosa was expressed in Escherichia coli and purified by Ni2+ chelate-affinity chromatography. After safety and pyrogenicity evaluations in animals, four groups of eight adult human volunteers were vaccinated intramuscularly three times at 4-week intervals and revaccinated 6 months later with either 500, 100, 50, or 20 microg of OprF-OprI adsorbed onto A1(OH)3. All vaccinations were well tolerated. After the first vaccination, a significant rise of antibody titers against P. aeruginosa OprF and OprI was measured in volunteers receiving the 100- or the 500-microg dose. After the second vaccination, significant antibody titers were measured for all groups. Elevated antibody titers against OprF and OprI could still be measured 6 months after the third vaccination. The capacity of the elicited antibodies to promote complement binding and opsonization could be demonstrated by a C1q-binding assay and by the in vitro opsonophagocytic uptake of P. aeruginosa bacteria. These data support the continued development of an OprF-OprI vaccine for use in humans.  (+info)

Lipopolysaccharide (LPS) from Burkholderia cepacia is more active than LPS from Pseudomonas aeruginosa and Stenotrophomonas maltophilia in stimulating tumor necrosis factor alpha from human monocytes. (8/9595)

Whole cells and lipopolysaccharides (LPSs) extracted from Burkholderia cepacia, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (TNF-alpha) from the human monocyte cell line MonoMac-6. B. cepacia LPS, on a weight-for-weight basis, was found to have TNF-alpha-inducing activity similar to that of LPS from E. coli, which was approximately four- and eightfold greater than the activity of LPSs from P. aeruginosa and S. maltophilia, respectively. The LPS-stimulated TNF-alpha production from monocytes was found to be CD14 dependent. These results suggest that B. cepacia LPS might play a role in the pathogenesis of inflammatory lung disease in cystic fibrosis, and in some patients it might be responsible, at least in part, for the sepsis-like cepacia syndrome.  (+info)

BioAssay record AID 164064 submitted by ChEMBL: Antibacterial activity against gram-negative bacteria Pseudomonas aeruginosa ATCC 27853; No effect.
Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide Academic Article ...
Cystic fibrosis (CF) lung disease begins in the first few months of life and follows a course of recurrent lower airway bacterial infection and inflammation and progression of disease over years and decades at a variable pace. With the development of chronic lung infection, obstructive disease progressively worsens, ultimately leading to respiratory failure. Pseudomonas aeruginosa (Pa) is the most important pathogen infecting the CF lower airways, and its acquisition early in life is associated with a pro-inflammatory effect, lower lung function, poor nutritional outcomes, and decreased survival.. Pseudomonas aeruginosa (Pa) infection of the cystic fibrosis (CF) airway typically proceeds from early infection to chronic infection. Although some studies have shown that a minority of individuals with CF spontaneously clear early Pseudomonas aeruginosa (Pa) infection, data from multiple studies suggest that antibiotics are superior to no treatment in clearing Pseudomonas aeruginosa (Pa) from ...
Many of the virulence factors produced by the opportunistic human pathogen Pseudomonas aeruginosa are quorum-sensing (QS) regulated. Among these are rhamnolipids, which have been shown to cause lysis of several cellular components of the human immune system, e.g. monocyte-derived macrophages and polymorphonuclear leukocytes (PMNs). We have previously shown that rhamnolipids produced by P. aeruginosa cause necrotic death of PMNs in vitro. This raises the possibility that rhamnolipids may function as a biofilm shieldin vivo, which contributes significantly to the increased tolerance of P. aeruginosa biofilms to PMNs. In the present study, we demonstrate the importance of the production of rhamnolipids in the establishment and persistence of P. aeruginosa infections, using an in vitro biofilm system, an intraperitoneal foreign-body model and a pulmonary model of P. aeruginosa infections in mice. Our experimental data showed that a P. aeruginosa strain, unable to produce any detectable ...
The opportunistic human pathogen Pseudomonas aeruginosa is the predominant micro-organism of chronic lung infections in cystic fibrosis patients. P. aeruginosa colonizes the lungs by forming biofilm microcolonies throughout the lung. Quorum sensing (QS) renders the biofilm bacteria highly tolerant t …
Pseudomonas aeruginosa and Aspergillus fumigatus are common opportunistic bacterial and fungal pathogens, respectively. They often coexist in airways of immunocompromised patients and individuals with cystic fibrosis, where they form biofilms and cause acute and chronic illnesses. Hence, the interactions between them have long been of interest and it is known that P. aeruginosa can inhibit A. fumigatusin vitro We have approached the definition of the inhibitory P. aeruginosa molecules by studying 24 P. aeruginosa mutants with various virulence genes deleted for the ability to inhibit A. fumigatus biofilms. The ability of P. aeruginosa cells or their extracellular products produced during planktonic or biofilm growth to affect A. fumigatus biofilm metabolism or planktonic A. fumigatus growth was studied in agar and liquid assays using conidia or hyphae. Four mutants, the pvdD pchE, pvdD, lasR rhlR, and lasR mutants, were shown to be defective in various assays. This suggested the P. aeruginosa ...
Pseudomonas aeruginosa lung infections are a major cause of death in cystic fibrosis and hospitalized patients. Treating these infections is becoming difficult due to the emergence of conventional antimicrobial multiresistance. While monosaccharides have proved beneficial against such bacterial lung infection, the design of several multivalent glycosylated macromolecules has been shown to be also beneficial on biofilm dispersion. In this study, calix[4]arene-based glycoclusters functionalized with galactosides or fucosides have been synthesized. The characterization of their inhibitory properties on Pseudomonas aeruginosa aggregation, biofilm formation, adhesion on epithelial cells, and destruction of alveolar tissues were performed. The antiadhesive properties of the designed glycoclusters were demonstrated through several in vitro bioassays. An in vivo mouse model of lung infection provided an almost complete protection against Pseudomonas aeruginosa with the designed glycoclusters.. ...
The opportunistic human pathogen Pseudomonas aeruginosa is the predominant micro-organism of chronic lung infections in cystic fibrosis patients. P. aeruginosa colonizes the lungs by forming biofilm microcolonies throughout the lung. Quorum sensing (QS) renders the biofilm bacteria highly tolerant to otherwise lethal doses of antibiotics, and protects against the bactericidal activity of polymorphonuclear leukocytes (PMNs). It has been previously demonstrated that QS is inhibited by garlic extract. In this study, the synergistic effects of garlic and tobramycin, and PMNs activities have been evaluated. P. aeruginosa was grown in vitro in continuous-culture once-through flow chambers with and without garlic extract. The garlic-treated biofilms were susceptible to both tobramycin and PMN grazing. Furthermore, the PMNs showed an increase in respiratory burst activation, when incubated with the garlic-treated biofilm. Garlic extract was administered as treatment for a mouse pulmonary infection model. Mice
Bacterial resistance to conventional antibiotics combined with an increasing acknowledgement of the role of biofilms in chronic infections has led to a growing interest in new antimicrobial strategies that target the biofilm mode of growth. In the aggregated biofilm mode, cell-to-cell communication systems involved in the process known as quorum sensing regulate coordinated expression of virulence with immune shielding mechanisms and antibiotic resistance. For two decades, the potential of interference with quorum sensing by small chemical compounds has been investigated with the aim of developing alternative antibacterial strategies. Here, we review state of the art research of quorum sensing inhibitors against the opportunistic human pathogen Pseudomonas aeruginosa, which is found in a number of biofilm-associated infections and identified as the predominant organism infecting the lungs of cystic fibrosis patients ...
TY - JOUR. T1 - Genome‐wide identification of novel small RNAs in Pseudomonas aeruginosa. AU - Gómez Lozano, María. AU - Marvig, Rasmus Lykke. AU - Molin, Søren. AU - Long, Katherine. PY - 2012. Y1 - 2012. N2 - Bacterial small regulatory RNAs (sRNAs) function in post‐transcriptional control of gene expression and control a variety of processes including metabolic reactions, stress responses and pathogenesis in response to environmental signals. A variety of approaches have been used previously to identify 44 sRNAs in the opportunistic human pathogen Pseudomonas aeruginosa. In this work, RNA sequencing (RNA‐seq) is used to identify novel transcripts in P. aeruginosa involving a combination of three different sequencing libraries. Almost all known sRNAs and over 500 novel intergenic sRNAs are identified with this approach. Although the use of three libraries increased the number of novel transcripts identified, there were significant differences in the subset of transcripts detected in ...
Phytopathology 102:575-587...Phytopathology 102:575-587...Effect of Overexpressing rsmA from Pseudomonas aeruginosa on Virulence of Select Phytotoxin-Producing Strains of P. syringae...Hye Suk Kong, Daniel P. Roberts, Cheryl D. Patterson, Sarah A. Kuehne, Stephan Heeb, Dilip K. Lakshman, and John Lydon...
Data of the study: Direct and indirect impact of the bacterial strain Pseudomonas aeruginosa on the dissolution of synthetic Fe(III)- and Fe(II)-bearing basaltic glasses
Introduction: Surgical wound infection is a serious problem, especially with metallo-beta lactamases (MBLs)- producing gram-negative bacteria as Pseudomonas aeruginosa. The main objective of this work was to evaluate for the first time in Minia- Upper Egypt, the incidence of imipenem-resistant Pseudomonas aeruginosa infection of surgical wounds particularly that mediated by MBL production.. Methodology: P. aeruginosa was isolated from infected wounds by swabs and underwent full microbiological identification and Antibiotic Susceptibility testing. MBL production was tested by E-test and PCR was used for imipenemase (blaIMP) and Verona integron-encoded metallo-beta-lactamase (blaVIM) gene detection.. Results: Out of 200 pus samples collected from surgical site infections, P. aeruginosa had the prevalence rate of 35%. Imipenem resistance was found in 28.57% of the isolated Pseudomonas aeruginosa. The prevalence of MBL-producing isolates among Imipenem-resistant P. aeruginosa (IRPA) was 85 % by ...
Cheats are a pervasive threat to public goods production in natural and human communities, as they benefit from the commons without contributing to it. Although ecological antagonisms such as predation, parasitism, competition, and abiotic environmental stress play key roles in shaping population biology, it is unknown how such stresses generally affect the ability of cheats to undermine cooperation. We used theory and experiments to address this question in the pathogenic bacterium, Pseudomonas aeruginosa Although public goods producers were selected against in all populations, our competition experiments showed that antibiotics significantly increased the advantage of nonproducers. Moreover, the dominance of nonproducers in mixed cultures was associated with higher resistance to antibiotics than in either monoculture. Mathematical modeling indicates that accentuated costs to producer phenotypes underlie the observed patterns. Mathematical analysis further shows how these patterns should ...
Ozer B, Duran N, Onlen Y, Savas L. Efflux pump genes and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from lower respiratory tract infections acquired in an intensive care unit. J Antibiot 2012; 65(1): 9-13. Badamchi, A., Masoumi, H., Javadinia, S., Asgarian, R. and Tabatabaie, A., 2017. Molecular detection of six virulence genes in Pseudomonas aeruginosa isolates detected in children with urinary tract infection. Microb Pathog 107: 44-47. Farra A, Islam S, Stralfors A, et al. Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem. Int J Antimicrob Agents 2008; 31(5): 427-33. Fazeli H, Havaei SA, Solgi H, et al. Pattern of antibiotic resistance in Pesudomonas aeruginosa isolated from intensive care unit, Isfahan, Iran. Journal of Isfahan Medical School 2013; 31(232): 432-438. Gutierrez O, Juan C, Cercenado E, et al. Molecular epidemiology and mechanisms of carbapenem resistance in Pseudomonas ...
A total of 3,700 Pseudomonas aeruginosa isolates were collected from 17 general hospitals in Japan from 1992 to 1994. Of these isolates, 132 carbapenem-resistant strains were subjected to DNA hybridization analysis with the metallo-beta-lactamase gene (blaIMP)-specific probe. Fifteen strains carrying the metallo-beta-lactamase gene were identified in five hospitals in different geographical areas. Three strains of P. aeruginosa demonstrated high-level imipenem resistance (MIC, , or = 128 micrograms/ml), two strains exhibited low-level imipenem resistance (MIC, , or = 4 micrograms/ml), and the rest of the strains were in between. These results revealed that the acquisition of a metallo-beta-lactamase gene alone does not necessarily confer elevated resistance to carbapenems. In several strains, the metallo-beta-lactamase gene was carried by large plasmids, and carbapenem resistance was transferred from P. aeruginosa to Escherichia coli by electroporation in association with the acquisition of the ...
Bacteria synchronize group behaviors using quorum sensing, which is advantageous during an infection to thwart immune cell attack and resist deleterious changes in the environment. In Pseudomonas aeruginosa, the Pseudomonas quinolone signal (Pqs) quorum-sensing system is an important component of an interconnected intercellular communication network. Two alkylquinolones, 2-heptyl-4-quinolone (HHQ) and 2-heptyl-3-hydroxy-4-quinolone (PQS), activate transcriptional regulator PqsR to promote the production of quinolone signals and virulence factors. Our work focused on the most abundant quinolone produced from the Pqs system, 2,4-dihydroxyquinoline (DHQ), which was shown previously to sustain pyocyanin production and antifungal activity of P. aeruginosa. However, little is known about how DHQ affects P. aeruginosa pathogenicity. Using C. elegans as a model for P. aeruginosa infection, we found pqs mutants only able to produce DHQ maintained virulence towards the nematodes similar to wild-type. In addition,
Haji SH. Detection of Biofilm Formation in Pseudomonas aeruginosa Isolates from Clinical Specimens. Zanco J Pure Appl Sci. 2018; 30[4]:83-89. doi: https://doi.org/10.21271/ZJPAS.30.4.9 Saha S, Devi KM, Damrolien S, Devi KS, . K, Sharma KT. Biofilm production and its correlation with antibiotic resistance pattern among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital in north-east India. Int J Adv Med. 2018;5[4]:964. doi: http://dx.doi.org/10.18203/2349-3933.ijam20183129 Vallés J, Mariscal D, Cortés P, Coll P, Villagrá A, Díaz E, et al. Patterns of colonization by Pseudomonas aeruginosa in intubated patients: A 3-year prospective study of 1,607 isolates using pulsed-field gel electrophoresis with implications for prevention of ventilator-associated pneumonia. Intensive Care Med. 2004; 30[9]:1768-1775. doi: https://doi.org/10.1007/s00134-004-2382-6. Gales AC, Jones RN, Turnidge J, Rennie R, Ramphal R. Characterization of Pseudomonas aeruginosa Isolates: Occurrence Rates, ...
The success of Pseudomonas aeruginosa in cystic fibrosis (CF) and other chronic infections is largely attributed to its ability to grow in antibiotic-resistant biofilm communities. This study investigated the effects of limiting iron levels as a strategy for preventing/disrupting P. aeruginosa biofilms. A range of synthetic and naturally occurring iron-chelating agents were examined. Biofilm development by P. aeruginosa strain PAO1 and CF sputum isolates from chronically infected individuals was significantly decreased by iron removal under aerobic atmospheres. CF strains formed poor biofilms under anaerobic conditions. Strain PAO1 was also tested under anaerobic conditions. Biofilm formation by this model strain was almost totally prevented by several of the chelators tested. The ability of synthetic chelators to impair biofilm formation could be reversed by iron addition to cultures, providing evidence that these effective chelating compounds functioned by directly reducing availability of iron to P.
An organism of concern, Pseudomonas aeruginosa is a water-loving bacteria that works and builds biofilms with other threatening bacteria. Understanding that disinfection alone will not rid an engineered water system of bacteria means we need to look toward biofilm-resistant material and disinfection at the source of use as opposed to where the water enters the building.. Antibiotic Resistance of Pseudomonas aeruginosa in Pneumonia at a Single University Hospital Center in Germany over a 10-Year Perioddetermines that while P. aeruginosa and MDR P. aeruginosa were resistant to a variety of commonly used antibiotics, they were not resistant to colistin in the few isolates recovered from patients with pneumonia.. Inhibition of Aspergillus fumigatus and Its Biofilm by Pseudomonas aeruginosa Is Dependent on the Source, Phenotype and Growth Conditions of the Bacterium reports that Aspergillus fumigatus (Af) and Pseudomonas aeruginosa (Pa) are leading fungal and bacterial pathogens, respectively, in ...
Cystic fibrosis lung disease is characterized by chronic airway infections with the opportunistic pathogen Pseudomonas aeruginosa and severe neutrophilic pulmonary inflammation. P. aeruginosa undergoes extensive genetic adaptation to the cystic fibrosis (CF) lung environment, and adaptive mutations in the quorum sensing regulator gene lasR commonly arise. We sought to define how mutations in lasR alter host-pathogen relationships. We demonstrate that lasR mutants induce exaggerated host inflammatory responses in respiratory epithelial cells, with increased accumulation of proinflammatory cytokines and neutrophil recruitment due to the loss of bacterial protease-dependent cytokine degradation. In subacute pulmonary infections, lasR mutant-infected mice show greater neutrophilic inflammation and immunopathology compared with wild-type infections. Finally, we observed that CF patients infected with lasR mutants have increased plasma interleukin-8 (IL-8), a marker of inflammation. These findings ...
Prime Journal of Microbiology Research (PJMR) ISSN: 2251-1261. susceptible and P. aeruginosa the least. Antibiotic use is suggested to be a major risk factor for.. chromID ™ P.aeruginosa Chromogenic medium for direct ID of Pseudomonas aeruginosa. Deliver rapid direct identification of Pseudomonas aeruginosa to contribute to.chromID™ P.aeruginosa Chromogenic medium for direct ID of Pseudomonas aeruginosa. Deliver rapid direct identification of Pseudomonas aeruginosa to contribute to.RESEARCH ARTICLE Open Access Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections Youenn Alexandre1, Rozenn Le Berre1,2*.tetracycline, Tetracycline is an antibiotic used to treat a number of bacterial infections. It is commonly used to treat acne and rosacea. Historically it was.Original article Antibiotic resistance and virulence properties of Pseudomonas aeruginosa strains from mechanically ventilated patients with pneumonia in intensive ...
BioAssay record AID 478497 submitted by ChEMBL: Antibacterial activity against Pseudomonas aeruginosa at 2 mg/ml after 24 hrs by agar diffusion method.
The opportunistic human pathogen Pseudomonas aeruginosa controls virulence, production of secondary metabolites, motility, biofilm formation, growth in anaerobic conditions, intracellular and intercellular signalling and the switch from an acute to a chronic mode of infection at the transcriptional and post-transcriptional levels by modulation of the Gac/Rsm system. Cell density-dependent signal accumulation and environmental stimulators such as pH changes and ion limitation activate the GacS/GacA two-component system which in turn triggers transcription of the small regulatory RNAs RsmY and RsmZ. These sRNAs sequester multiple copies of the RNA-binding protein RsmA, antagonising its function. The RsmA/CsrA proteins act as translational repressors by binding to the GGA-motifs in the untranslated region of target mRNAs and blocking ribosome binding. In this study, the biological function of RsmN, an RsmA homologue with a conserved RNA-binding pocket but a distinct protein folding, the predicted ...
EDTA has a detrimental effect on the outer membrane permeability of free-living planktonic Proteobacteria (15, 25, 29, 40). By chelating divalent cations from their binding sites in lipopolysaccharide (LPS), EDTA facilitates the release of a significant proportion of LPS from the cell (26). Although prolonged treatments with EDTA are lethal, short treatments increase the permeability of the outer membrane to hydrophobic molecules (25, 29). Thus, there can be synergy between EDTA and other antibacterial agents (2, 8, 24). In this study we report that EDTA not only kills P. aeruginosa planktonic cells but also affects P. aeruginosa biofilms (Fig. 1 and 2).. Exposure of P. aeruginosa biofilms to EDTA killed P. aeruginosa cells and triggered detachment of cells from biofilms (Fig. 3 to 5). CSLM revealed that the majority of the cell population affected by the EDTA treatment resides in the inner regions of the mushroom-like structures. This type of killing or detachment pattern has been observed in ...
Increasing rates of antibiotic resistance among Gram-negative pathogens such as Pseudomonas aeruginosa means alternative approaches to antibiotic development are urgently required. Pyocins, produced by P. aeruginosa for intraspecies competition, are highly potent protein antibiotics known to actively translocate across the outer membrane of P. aeruginosa. Understanding and exploiting the mechanisms by which pyocins target, penetrate and kill P. aeruginosa is a promising approach to antibiotic development. In this work we show the therapeutic potential of a newly identified tRNase pyocin, pyocin SD2, by demonstrating its activity in vivo in a murine model of P. aeruginosa lung infection. In addition, we propose a mechanism of cell targeting and translocation for pyocin SD2 across the P. aeruginosa outer membrane. Pyocin SD2 is concentrated at the cell surface, via binding to the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide (LPS), from where it can efficiently locate its outer
Increasing rates of antibiotic resistance among Gram-negative pathogens such as Pseudomonas aeruginosa means alternative approaches to antibiotic development are urgently required. Pyocins, produced by P. aeruginosa for intraspecies competition, are highly potent protein antibiotics known to actively translocate across the outer membrane of P. aeruginosa. Understanding and exploiting the mechanisms by which pyocins target, penetrate and kill P. aeruginosa is a promising approach to antibiotic development. In this work we show the therapeutic potential of a newly identified tRNase pyocin, pyocin SD2, by demonstrating its activity in vivo in a murine model of P. aeruginosa lung infection. In addition, we propose a mechanism of cell targeting and translocation for pyocin SD2 across the P. aeruginosa outer membrane. Pyocin SD2 is concentrated at the cell surface, via binding to the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide (LPS), from where it can efficiently locate its ...
Pseudomonas aeruginosa ATCC ® 47085D-5™ Designation: Genomic DNA from Pseudomonas aeruginosa strain PAO1-LAC TypeStrain=False Application:
Cluster II che Genes from Pseudomonas aeruginosa Are Required for an Optimal Chemotactic Response: Pseudomonas aeruginosa, a γ-proteobacterium, is motile by mea
Bacterial viruses, or phage, are key members of natural microbial communities. Yet much research on bacterial-phage interactions has been conducted in liquid cultures involving single bacterial strains. Here we explored how bacterial diversity affects the success of lytic phage in structured communities. We infected a sensitive Pseudomonas aeruginosa strain PAO1 with a lytic phage Pseudomonas 352 in the presence versus absence of an insensitive P. aeruginosa strain PA14, in liquid culture versus colonies on agar. We found that both in liquid and in colonies, inter-strain competition reduced resistance evolution in the susceptible strain and decreased phage population size. However, while all sensitive bacteria died in liquid, bacteria in colonies could remain sensitive yet escape phage infection, due mainly to reduced growth in colony centers. In sum, spatial structure can protect bacteria against phage infection, while the presence of competing strains reduces the evolution of resista
Shape (Cocci/Diplococci/Rods) Rods. of a 1% solution of α-naphthol in 95% … Pseudomonas aeruginosa. This enzyme oxidizes di-methyl-p-phenylenediamine in the presence of molecular oxygen and cytochrome c and on the addition of α-naphthol, indophenol blue is formed. After filtration of the sample and a short cultivation step, this test confirms all grown microcolonies, not only a selection as it is the case with classical methods. Negative. Item Number: 01230002. Properties (Pseudomonas aeruginosa) Capsule. Dentalanalyse Pseudomonas Dieser Test untersucht folgende Parameter: Mikrobiologischer Parameter: Pseudomonas aeruginosa Schritt 1: Innerhalb eines Werktages verschicken wir Ihre Bestellung! I got the HI MEDIA Biochemical test results as follows: Citrate test +ve, Lysine +ve, Ornithine +ve, Urease +ve, Phenylalanine deamination -ve, Nitrate reduction -ve, H2S Production +ve, Glucose -ve, Adonitol-ve, Lactose -ve, Arabinose +ve, Sorbitol-ve. Pseudomonas aeruginosa colonies that were ...
Pseudomonas aeruginosa Serotype 2B antibody LS-C538938 is an FITC-conjugated mouse monoclonal antibody to pseudomonas aeruginosa Pseudomonas aeruginosa Serotype 2B. Validated for ELISA.
TY - JOUR. T1 - Dual β-lactam combination therapy for multi-drug resistant Pseudomonas aeruginosa infection. T2 - enhanced efficacy in vivo and comparison with monotherapies of penicillin-binding protein inhibition. AU - Siriyong, Thanyaluck. AU - Murray, Rachael M.. AU - Bidgood, Lucy E.. AU - Young, Simon A.. AU - Wright, Florence. AU - Parcell, Benjamin J.. AU - Voravuthikunchai, Supayang Piyawan. AU - Coote, Peter J.. PY - 2019/6/24. Y1 - 2019/6/24. N2 - The aim of the study was to determine the efficacy of dual β-lactam combination treatments derived from eight approved drugs against Galleria mellonella larvae infected with MDR strains of P. aeruginosa. Carbapenem-resistant P. aeruginosa NCTC 13437 and an unrelated clinical isolate were used to infect G. mellonella larvae and the efficacy of twenty-eight dual β-lactam combination therapies were compared to their constituent monotherapies. For the most potent combinations identified, penicillin-binding protein (PBP) inhibition profiles ...
In the opportunistic pathogen Pseudomonas aeruginosa, quorum sensing (QS) via acyl-homoserine lactone (AHL) signals coordinates virulence gene expression. AHL signals must reach a critical threshold before enough is bound by cognate regulators LasR and RhlR to drive transcription of target genes. In addition, three anti-activator proteins, QteE, QscR, and QslA, sequester QS regulators to increase the threshold for induction and delay expression of QS target genes. It remains unclear how multiple anti-activators work together to achieve the quorum threshold. Here, we employed a combination of mutational, kinetic, phenotypic, and transcriptomic analysis to examine regulatory effects and interactions of the three distinct anti-activators. We observed combinatorial, additive effects on QS gene expression. As measured by reporter gene fusion, individual deletion of each anti-activator gene increased lasB expression and QS-controlled virulence factor production. Deletion of qslA in combination with the
A Pf1-like phage is involved in P. aeruginosa biofilm killing.Electron microscopic examination of the CsCl-purified phage revealed filamentous phage particles that were approximately 1.5 μm long (Fig. 3b). The genome of P. aeruginosa contains a filamentous prophage that is closely related to phage Pf1, and Pf1 genes are known to be upregulated in P. aeruginosa biofilms (66). Moreover, it is known that Pf1 can infect a cell by using T4P (24). Flagella have also been reported to be receptors for filamentous phage (44), and our data suggest that the P. aeruginosa Pf1-like phage may additionally infect a cell through the flagellum. We also carried out PCR with Pf1-specific primers 437F and 437R using DNA extracted from the CsCl-purified phage band. The 894-bp PCR product was sequenced, and the sequence showed 100% identity with the sequence of the Pf1-like prophage from P. aeruginosa. We also hybridized a PCR-labeled, Pf1-specific DNA probe with individual plaques generated from the biofilm ...
A Pf1-like phage is involved in P. aeruginosa biofilm killing.Electron microscopic examination of the CsCl-purified phage revealed filamentous phage particles that were approximately 1.5 μm long (Fig. 3b). The genome of P. aeruginosa contains a filamentous prophage that is closely related to phage Pf1, and Pf1 genes are known to be upregulated in P. aeruginosa biofilms (66). Moreover, it is known that Pf1 can infect a cell by using T4P (24). Flagella have also been reported to be receptors for filamentous phage (44), and our data suggest that the P. aeruginosa Pf1-like phage may additionally infect a cell through the flagellum. We also carried out PCR with Pf1-specific primers 437F and 437R using DNA extracted from the CsCl-purified phage band. The 894-bp PCR product was sequenced, and the sequence showed 100% identity with the sequence of the Pf1-like prophage from P. aeruginosa. We also hybridized a PCR-labeled, Pf1-specific DNA probe with individual plaques generated from the biofilm ...
Looking for Pseudomonas aeruginosa? Find out information about Pseudomonas aeruginosa. An opportunistic pathogen that is the most significant cause of hospital-acquired infections, particularly in predisposed patients with metabolic,... Explanation of Pseudomonas aeruginosa
ABSTRACT. The present work attempts to solve pollution problems in watery surroundings by aromatic compounds such as the phenol and the benzoic acid. Several ways of elimination of these compounds were the object of different research among which is the use of bacteria. In this framework, Pseudomonas aeruginosa bacterium is used to eliminate phenol and the benzoic acid. This made it possible to isolate the Pseudomonas aeruginosa bacterium directly on the nourishing environment containing phenol and benzoic acid as source of energy then the bacteria is incubated at 37˚C during a minimal duration of four days. Furthermore, we studied the influence of the Pseudomonas aeruginosa bacterium on the deterioration of an area exposed to a phenol and the benzoic acid concentration. Results obtained at the time of the different experimentations clearly show that phenol and the benzoic acid were eliminated by the Pseudomonas aeruginosa bacterium. However, it was noted that during the various investigations ...
The early endobronchial inflammation induced by Pseudomonas aeruginosa infection varies in resistant and susceptible strains of mice. Mice of the DBA/2 strain are severely afflicted by the infection, with a high bacterial burden accumulating rapidly following inoculation and a high mortality rate occurring. Mice of the BALB/c strain are resistant to infection and clear the bacteria within 3 to 7 days. Infection of (BALB/c x DBA/2)F1 hybrid mice showed that the resistance to lung P. aeruginosa infection is inherited as a dominant trait. Mice of the A/J and C57BL/6 strains were found to have an intermediate phenotype to Pseudomonas aeruginosa infection when compared with BALB/c and DBA/2 strains. The decrease in the bacterial load seen early after infection coincided with a steady and strong recruitment of inflammatory cells to the bronchoalveolar spaces of mice of the resistant BALB/c strain. On the other hand, the recruitment of inflammatory cells to the lungs of mice of the susceptible DBA/2 ...
TY - JOUR. T1 - Slime production a virulence marker in Pseudomonas aeruginosa strains isolated from clinical and environmental specimens. T2 - A comparative study of two methods. AU - Prasad, S. Vishnu. AU - Ballal, Mamatha. AU - Shivananda, P. G.. PY - 2009/4/1. Y1 - 2009/4/1. N2 - Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism. Here, comparative studies of two phenotypic methods using the tube method and the spectrophotometric method for slime production from 100 clinically and 21 environmentally significant isolates of P. aeruginosa were performed. A total of 68 isolates were positive by either of the tests whereas only 34 were positive by both the tests. The tube method detected slime significantly in more number of isolates than the spectrophotometric method. The tube test was found to be superior to the spectrophotometric method in ease of performance, interpretation and sensitivity. Among the clinical isolates, systemic isolates ...
Background: Pseudomonas aeruginosa is one of the primary pathogens isolated more frequently in cystic fibrosis (CF) and it exhibits innate resistance to a wide range of antibiotics. Purpose: We sought to determine whether the highly prevalent genotypes of P. aeruginosa are specifically linked to CF patients and have any related multidrug antibiotic resistance. Isolates from hospitalized non-CF patients and from environmental sources were also genotypically analyzed. Methods: Collections of P. aeruginosa from lower respiratory secretions (n=45) were genotyped using pulsed-field gel electrophoresis (PFGE). Phenotypic screening for antibiotic susceptibility was performed for the common antimicrobial agents by E-test and automated Phoenix method. Results: P. aeruginosa isolates from CF (n=32), hospitalized non-CF patients (n=13), and environment sources (n=5) were analyzed. The population structure of P. aeruginosa is highly diverse and population-specific. All PFGE results of P. aeruginosa isolates ...
A total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance analysis. Genotyping showed that 104 (56.8%) isolates belonged to a single major clone (clone B), which was identified by multilocus sequence typing as sequence type (ST) 175. This clone was initially isolated from 5 patients in 2008, and then isolated from 23 patients in 2009 and 76 patients in 2010. PCR analysis of clone B isolates identified the bla(VIM-2) gene in all but 1 isolate, which harbored bla(IMP-22). ST175 isolates were susceptible to only amikacin (75%) and colistin (100%). Emergence of the ST175 clone represents a major health problem because it compromises therapy for treatment of P. aeruginosa nosocomial infections ...
One of the major clinical problems regarding Pseudomonas aeruginosa is attributed to metallo-beta-lactamases (MBL). This group of enzymes is a subset of beta lactamases which belong to group B of Ambler classification and cause hydrolysis of carbapenems. Based on epidemiological studies conducted worldwide, it is proved that prevalence of genes coding MBLs in P. aeruginosa species are different in various geographic zones and even in various hospitals. Therefore, according to the clinical importance of organisms generating MBLs, it is necessary to identify and control these bacteria in hospitals for therapeutic purposes.The current study aimed to investigate the Metallo-beta-Lactamase VIM-1, SPM-1, and IMP-1 genes among clinical P. aeruginosa species isolated in Zahedan, Iran.The current study investigated the presence of MBL through phenotypic and genotypic methods and also the pattern of antibiotic resistance in P. aeruginosa species isolated in hospitals. The Minimum Inhibitory Concentration (MIC)
Los mecanismos innatos y adquiridos de resistencia a los antibióticos en Pseudomonas representan un reto para los médicos que buscan una quimioterapia oportuna y eficaz. Esto es par- ticularmente importante en las áreas de cuidados intesnsivos de los hospitales. Este estudio está dirigido a lograr una comprensión a nivel molecular de dos de los más importantes mecanismos de resistencia a los fármacos en Pseudomonas aeruginosa. Cien aislados clínicos de Pseudomonas aeruginosa se obtuvieron de un hospital de tercer nivel en Quito, Ecuador. Se analizó la expresión de ampC y oprD mediante PCR cuantitativa en tiempo real. Se realizó una comparación entre los perfiles de expresión ampC y oprD y los fenotipos obtenidos en la prueba de susceptibilidad antimicrobiana (AST), con más del 50% de los aislados con perfiles concordantes para la expresión ampC y oprD. Nuestros resultados sugieren que la expresión ampC y oprD podría proporcionar información útil sobre mecanismos de resistencia ...
Pseudomonas aeruginosa causes aggressive infection in patients with pre-existing disorders and recurrent pulmonary infections in cystic fibrosis patients. Pathogenesis of P. aeruginosa infections is multifactorial owing to numerous virulence factors. The focus of this thesis research was to investigate whether P. aeruginosa elastase (PE) causes remodeling of the cytoskeleton by increasing the phosphorylation of RhoA GTPase proteins. In addressing our hypothesis, we utilized Small GTPase Immuno-sorbent Activation assays (G-LISA) and Enzyme linked Immuno-sorbent assay (ELISA) to quantitate changes in the total as well as phosphorylated RhoA protein in Calu3 cell lines. Fluorescence microscopy aided in understanding the changes in morphological organization of F-actin. Changes in expression of TJ protein, ZO1, due to PE induced RhoA GTPase activity, was analyzed with SDS PAGE and Western Blot Analysis. Our data from G-LISA and ELISA assays indicate that PE increases the amount of active RhoA protein by 50
Emond et al are reporting in Nat Genetics the identification of DCTN4 as a modifier for P.aeruginosa infection in patients with cystic fibrosis. It is a well-established fact that the majority of patients with cystic fibrosis develop acute and chronic P.aerugonisa infections which are associated with a worse clinical outcome. The authors selected and exome sequenced 91 patients from the EPIC collection with cystic fibrosis and P.aeruginosa and after performing logistic regression adjusted for ancestry and for CFTR mutation risk group identified DCTN4 as the only modifier gene. Dynactin 4 is a component of the dynein-dependent motor that moves autophagosomes along microtubules into lysosomes for degradation as part of the autophagy process which has an essential role in the clearance of P. aeruginosa. The presence of at least one DCTN4 missense variant was significantly associated with both early age of first P. aeruginosaâ€positive culture and with early age at onset of chronic P. aeruginosa ...
The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.
Author Summary Pathogens face a hostile and often novel environment when infecting a new host, and adaptation to this environment can be critical to a pathogens survival. The genetic basis of pathogen adaptation is in turn important for treatment, since the consistency with which therapies succeed may depend on the extent to which a pathogen adapts via the same routes in different patients. In this study, we investigate adaptation of the bacterium Pseudomonas aeruginosa to laboratory conditions that resemble the lungs of cystic fibrosis patients and to quinolone antibiotics. We find that a handful of genes and genetic pathways are repeatedly involved in adaptation to each condition. Nonetheless, other, less common mutations can play important roles in determining fitness, complicating strategies aimed at reducing the prevalence of antibiotic resistance.
Decreased Pseudomonas aeruginosa biofilm formation on nanomodified endotracheal tubes: a dynamic lung model Mary C Machado,1 Thomas J Webster2 1Center for Biomedical Engineering, Division of Engineering Brown University, RI, USA; 2Department of Orthopaedics, Division of Engineering Brown University, RI, USA Abstract: Ventilator-associated pneumonia (VAP) is a serious complication of mechanical ventilation that has been shown to be associated with increased mortality rates and medical costs in the pediatric intensive care unit. Currently, there is no cost-effective solution to the problems posed by VAP. Endotracheal tubes (ETTs) that are resistant to bacterial colonization and that inhibit biofilm formation could provide a novel solution to the problems posed by VAP. The objective of this in vitro study was to evaluate differences in the growth of Pseudomonas aeruginosa on unmodified polyvinyl chloride (PVC) ETTs and on ETTs etched with a fungal lipase, Rhizopus arrhizus, to create nanoscale surface
Pseudomonas aeruginosa is a gram-negative opportunistic pathogen in patients with neutropenia, cystic fibrosis, and burn wounds (1, 15, 19, 21). The prevalence of multidrug-resistant strains complicates the control ofP. aeruginosa (3), which has prompted studies to define the molecular basis for its pathogenesis. P. aeruginosa possesses an array of virulence factors, which makes it a successful opportunistic pathogen (6), including the ADP-ribosyltransferases, exotoxin A, and exoenzyme S.. Exoenzyme S was identified by Iglewski and coworkers as an ADP-ribosyltransferase of P. aeruginosa (8). Cloning the two forms of exoenzyme S showed that the 53-kDa form of exoenzyme S (now termed exoenzyme T [ExoT]) and the 49-kDa form of exoenzyme S (now termed exoenzyme S [ExoS]) were encoded by separate genes that were located on the P. aeruginosa chromosome (10, 22). While alignment of their primary amino acid sequences showed that ExoS and ExoT possess 76% homology (22), the specific activity of ExoT in ...
Taxobox , color = lightgrey , name = Pseudomonas , image = Pseudomonas aeruginosa 01.jpg , image_width = 280px , image_caption = P. aeruginosa colonies on an [[agar plate]]. , regnum = [[Bacterium,Bacteria]] , phylum = [[Proteobacteria]] , classis = [[Proteobacteria,Gamma Proteobacteria]] , ordo = [[Pseudomonadales]] , familia = [[Pseudomonadaceae]] , genus = Pseudomonas , genus_authority = Migula 1894 , type_species = [[Pseudomonas aeruginosa]] , subdivision_ranks = Species , subdivision = P. aeruginosa group :[[Pseudomonas aeruginosa,P. aeruginosa]] :[[Pseudomonas alcaligenes,P. alcaligenes]] :[[Pseudomonas anguilliseptica,P. anguilliseptica]] :[[Pseudomonas argentinensis,P. argentinensis]] :[[Pseudomonas borbori,P. borbori]] :[[Pseudomonas citronellolis,P. citronellolis]] :[[Pseudomonas flavescens,P. flavescens]] :[[Pseudomonas mendocina,P. mendocina]] :[[Pseudomonas nitroreducens,P. nitroreducens]] :[[Pseudomonas ...
Biofilms have been implicated as an important reservoir for pathogens and commensal enteric bacteria such as Escherichia coli in natural and engineered water systems. However, the processes that regulate the survival of E. coli in aquatic biofilms have not been thoroughly studied. We examined the effects of hydrodynamic shear and nutrient concentrations on E. coli colonization of pre-established Pseudomonas aeruginosa biofilms, co-inoculation of E. coli and P. aeruginosa biofilms, and P. aeruginosa colonization of pre-established E. coli biofilms. In nutritionally-limited R2A medium, E. coli dominated biofilms when co-inoculated with P. aeruginosa, and successfully colonized and overgrew pre-established P. aeruginosa biofilms. In more enriched media, P. aeruginosa formed larger clusters, but E. coli still extensively overgrew and colonized the interior of P. aeruginosa clusters. In mono-culture, E. coli formed sparse and discontinuous biofilms. After P. aeruginosa was introduced to these biofilms, E.
TY - JOUR. T1 - Chronic colonization of rat airways with Pseudomonas aeruginosa. AU - Boyd, R. L.. AU - Ramphal, R.. AU - Rice, R.. AU - Mangos, J. A.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - Colonization of the airways of rats by Pseudomonas aeruginosa was established by treating the animals with hexamethylphosphoramide (HMPA) and inoculating with P. aeruginosa. Male Sprague-Dawley rats were given tap water (controls) or HMPA in the drinking water at 2 or 4 mg/ml. The ciliated cells of the airway epithelium were denuded, and microulcerative lesions in the epithelium were induced in the HMPA-treated rats. After 2 weeks of treatment, the rats were inoculated by transoral intratracheal instillation with 5 x 107 CFU of P. aeruginosa obtained from a cystic fibrosis patient. Two weeks after inoculation, P. aeruginosa was cultured from the airways, and scanning and transmission electron microscopy showed bacilli adhering to or invading the injured airway epithelium. P. aeruginosa was present in tracheal ...
Multilocus amplicon sequencing of Pseudomonas aeruginosa cystic fibrosis airways isolates collected prior to and after early antipseudomonal ...
TY - JOUR. T1 - Reverting Antibiotic Tolerance of Pseudomonas aeruginosa PAO1 Persister Cells by (Z)-4-bromo-5-(bromomethylene)-3-methylfuran-2(5H)-one. AU - Pan, Jiachuan. AU - Bahar, Ali Adem. AU - Syed, Haseeba. AU - Ren, Dacheng. N1 - Copyright: Copyright 2013 Elsevier B.V., All rights reserved.. PY - 2012/9/20. Y1 - 2012/9/20. N2 - Background: Bacteria are well known to form dormant persister cells that are tolerant to most antibiotics. Such intrinsic tolerance also facilitates the development of multidrug resistance through acquired mechanisms. Thus persister cells are a promising target for developing more effective methods to control chronic infections and help prevent the development of multidrug-resistant bacteria. However, control of persister cells is still an unmet challenge. Methodology/Principal Findings: We show in this report that (Z)-4-bromo-5-(bromomethylene)-3-methylfuran-2(5H)-one (BF8) can restore the antibiotic susceptibility of Pseudomonas aeruginosa PAO1 persister cells ...
Soluble (S-type) pyocins are Pseudomonas aeruginosa bacteriocins that kill nonimmune P. aeruginosa strains via a specific receptor. The genes coding for pyocin Sa (consisting of a killing protein and an immunity protein) were cloned and expressed in Escherichia coli. Sequence analysis revealed that Sa is identical to pyocin S2. Seventy-nine strains of P. aeruginosa were tested for their sensitivity to pyocins S1, S2, and S3, and their ferripyoverdine receptors were typed by multiplex PCR. No strain was found to be sensitive to both S2 and S3, suggesting that the receptors for these two pyocins cannot coexist in one strain. As expected, all S3-sensitive strains had the type II ferripyoverdine receptor fpvA gene, confirming our previous reports. S1 killed strains irrespective of the type of ferripyoverdine receptor they produced. All S2-sensitive strains had the type I fpvA gene, and the inactivation of type I fpvA in an S2-sensitive strain conferred resistance to the S2 pyocin. Accordingly, ...
Аннотация: The properties of the isolated Pseudomonas aeruginosa bacteriophage phiPMG1 include the lytic infection cycle, and the formation of a broad halo (semi-transparent zone) around the plaques. We consider phiPMG1 as a potential member of therapeutic cocktails of live phages, and as a source of peptidoglycan and lipopolysaccharide degrading enzymes. Partial sequencing of phiPMG1 genome has revealed high similarity with known temperate P. aeruginosa phage D3. An open reading frame encoding lytic transglycosilase was identified in the genome. This enzyme PMG MUR was obtained in recombinant form, and its activity and substrate specificity has been studied. ...
TY - JOUR. T1 - Pseudomonas aeruginosa potentiates the lethal effect of intestinal ischemia-reperfusion injury. T2 - The role of in vivo virulence activation. AU - Fink, David. AU - Romanowski, Kathleen. AU - Valuckaite, Vesta. AU - Babrowski, Trissa. AU - Kim, Moses. AU - Matthews, Jeffrey B.. AU - Liu, Donald. AU - Zaborina, Olga. AU - Alverdy, John C.. PY - 2011/12/1. Y1 - 2011/12/1. N2 - Background: Experimental models of intestinal ischemia-reperfusion (IIR) injury are invariably performed in mice harboring their normal commensal flora, even though multiple IIR events occur in humans during prolonged intensive care confinement when they are colonized by a highly pathogenic hospital flora. The aims of this study were to determine whether the presence of the human pathogen Pseudomonas aeruginosa in the distal intestine potentiates the lethality of mice exposed to IIR and to determine what role any in vivo virulence activation plays in the observed mortality. Methods: Seven- to 9-week-old ...
AIMS: To establish the ability of the rhamnolipids biosurfactants from Pseudomonas aeruginosa, in the presence and absence of caprylic acid and ascorbic acid, to disrupt bacterial biofilms, compared with the anionic alkyl sulphate surfactant Sodium dodecyl sulphate (SDS). METHODS AND RESULTS: Pseudomonas aeruginosa ATCC 15442 biofilms were disrupted by rhamnolipids at concentrations between 0·5 and 0·4 g l(-1) and with SDS at 0·8 g l(-1) . The combination of rhamnolipids 0·4 g l(-1) and caprylic acid at 0·1 g l(-1) showed a remarkable effect on biofilm disruption and cell killing. After 30 min of treatment most of the biofilm was disrupted and cell viability was significantly reduced. Neither caprylic acid nor ascorbic acid has any effect on biofilm disruption at 0·1 g l(-1) . SDS is an effective antimicrobial agent; however, in the presence of caprylic acid its effect was neutralized. CONCLUSIONS: The results show that rhamnolipids at low concentration in the presence of caprylic acid are ...
TY - JOUR. T1 - Resistance emergence mechanism and mechanism of resistance suppression by tobramycin for cefepime for Pseudomonas aeruginosa. AU - Drusano, G. L.. AU - Bonomo, Robert A.. AU - Bahniuk, Nadzeya. AU - Bulitta, Juergen B.. AU - VanScoy, Brian. AU - DeFiglio, Holland. AU - Fikes, Steven. AU - Brown, David. AU - Drawz, Sarah M.. AU - Kulawy, Robert. AU - Louie, Arnold. N1 - Copyright: Copyright 2012 Elsevier B.V., All rights reserved.. PY - 2012/1. Y1 - 2012/1. N2 - The panoply of resistance mechanisms in Pseudomonas aeruginosa makes resistance suppression difficult. Defining optimal regimens is critical. Cefepime is a cephalosporin whose 3′ side chain provides some stability against AmpC β-lactamases. We examined the activity of cefepime against P. aeruginosa wild-type strain PAO1 and its isogenic AmpC stably derepressed mutant in our hollow-fiber infection model. Dose-ranging studies demonstrated complete failure with resistance emergence (both isolates). Inoculum range studies ...
Cystic fibrosis (CF) is a complex inherited disease which affects many organs, including the pancreas and liver, gastrointestinal tract and reproductive system, sweat glands and, particularly, the respiratory system. Pseudomonas aeruginosa is the main cause of chronic airway infection. In order to reduce morbidity and mortality due to lung infection by P. aeruginosa, aerosol antibiotics have been used to achieve high local concentrations in the airways and to reduce systemic toxicity. In the course of this review, the current treatments to control CF lung infections by P. aeruginosa are presented. Some innovative aerosol formulations such as liposomes and microspheres are herein reviewed, which may improve the efficiency of anti-pseudomonal agents, and ensure patients compliance to treatments, by reducing dosing frequency and/or drug dose, while maintaining therapeutic efficacy, preventing the occurrence of bacterial resistance and/or reducing adverse effects due to their controlled-release ...
Pseudomonas aeruginosa bacteriophage 11 ATCC ® 14205-B1™ Designation: Phage 11 TypeStrain=False Application: Phage therapy research Ref
The incidence of Pseudomonas aeruginosa bacteraemia (PAB) has remained stable over the last few decades.1-3 Although it is still primarily a nososcomial infection, the number of cases of community-acquired bacteraemia caused by this organism has increased, notably affecting patients with AIDS4,5 and neutropenic patients treated for neoplastic disease who received outpatient management.6 Predisposing conditions for PAB include compromised immunity, neutropenia, intensive care, surgical procedures, central venous and urinary catheters and previous cephalosporin therapy.1,3-5,6 Common factors predictive of a fatal outcome reported in the literature are septic shock, neutropenia, immunocompromised state, severe underlying disease, and in the elderly pneumonia, septic metastases, previous therapy and inappropriate choice of antimicrobial drugs for definitive treatment.1,6,7. P. aeruginosa has also emerged as an important bacteraemic pathogen in immunocompromised children,6,8,9 including ...
The incidence of Pseudomonas aeruginosa bacteraemia (PAB) has remained stable over the last few decades.1-3 Although it is still primarily a nososcomial infection, the number of cases of community-acquired bacteraemia caused by this organism has increased, notably affecting patients with AIDS4,5 and neutropenic patients treated for neoplastic disease who received outpatient management.6 Predisposing conditions for PAB include compromised immunity, neutropenia, intensive care, surgical procedures, central venous and urinary catheters and previous cephalosporin therapy.1,3-5,6 Common factors predictive of a fatal outcome reported in the literature are septic shock, neutropenia, immunocompromised state, severe underlying disease, and in the elderly pneumonia, septic metastases, previous therapy and inappropriate choice of antimicrobial drugs for definitive treatment.1,6,7. P. aeruginosa has also emerged as an important bacteraemic pathogen in immunocompromised children,6,8,9 including ...
SE «Dnepropetrovsk Medical Academy, Ministry of Health of Ukraine», Dnepr, Ukraine The article based on the literature demonstrated a role in the development of cellular immune reactions in response pneumonia caused by Pseudomonas aeruginosa. The mechanisms described of recruitment and activation of pro-inflammatory immune cells, killing the bacterial processes that ensure effective sanogenesis infection by Pseudomonas aeruginosa and prevent the formation of a chronic inflammatory process. Key words: pneumonia, Pseudomonas aeruginosa, bacterial killing, immune cells References 1. Abaturov AE. 2009. Meaning metallosvyazyvayuschih nonspecific proteins in protection of the respiratory tract. 1. Lactoferrin. Child Health. 4(19): 125-128. 2. Abaturov AE, Volosovets AP, Yulish EI. 2013. The role of prooxidant and antioxidant systems in inflammatory diseases of the respiratory. Kharkov, Planet Print: 496. 3. Abaturov AE, Gerasimenko ON, Vysochina IL, Zavgorodnyaya NJ. 2011. Defensins and ...
TY - JOUR. T1 - Inhaled aztreonam lysine for chronic airway Pseudomonas aeruginosa in cystic fibrosis. AU - McCoy, Karen S.. AU - Quittner, Alexandra L.. AU - Oermann, Christopher M.. AU - Gibson, Ronald L.. AU - Retsch-Bogart, George Z.. AU - Montgomery, A. Bruce. PY - 2008/11/1. Y1 - 2008/11/1. N2 - Rationale: The effectiveness and safety of aztreonam lysine for inhalation (AZLI) in patients with cystic fibrosis (CF) on maintenance treatment for Pseudomonas aeruginosa (PA) airway infection was evaluated in this randomized, double-blind, placebo-controlled study. Objectives: To evaluate the safety and efficacy of inhaled aztreonam lysine in controlling PA infection in patients with CF. Methods: After randomization and a 28-day course of tobramycin inhalation solution (TIS), patients (n = 211; ≥6yr; ≥3 TIS courses within previous year; FEV1 ≥25% and ≤75% predicted values) were treated with 75 mg AZLI or placebo, twice or three times daily for 28 days, then monitored for 56 days. The ...
Clearance of neutrophils from inflamed sites is critical for resolution of inflammation, but pathogen-driven neutrophil apoptosis can impair host defenses. We previously showed that pyocyanin, a phenazine toxic metabolite produced by Pseudomonas aeruginosa, accelerates neutrophil apoptosis in vitro. We compared wild-type and pyocyanin-deficient strains of P. aeruginosa in a murine model of acute pneumonia. Intratracheal instillation of either strain of P. aeruginosa caused a rapid increase in bronchoalveolar lavage neutrophil counts up to 18 h after infection. In wild-type infection, neutrophil numbers then declined steadily, whereas neutrophil numbers increased up to 48 h in mice infected with pyocyanin-deficient P. aeruginosa. In keeping with these differences, pyocyanin production was associated with reduced bacterial clearance from the lungs. Neutrophil apoptosis was increased in mice infected with wild-type compared with the phenazine-deficient strain or two further strains that lack ...
Understanding how the folding of proteins establishes their functional characteristics at the molecular level challenges both theorists and experimentalists. The simplest test beds for confronting this issue are provided by electron transfer proteins. The environment provided by the folded protein to the cofactor tunes the metals electron transport capabilities as envisioned in the entatic hypothesis. To see how the entatic state is achieved one must study how the folding landscape affects and in turn is affected by the metal. Here, we develop a coarse-grained functional to explicitly model how the coordination of the metal (which results in a so-called entatic or rack-induced state) modifies the folding of the metallated Pseudomonas aeruginosa azurin. Our free-energy functional-based approach directly yields the proper nonlinear extra-thermodynamic free energy relationships for the kinetics of folding the wild type and several point-mutated variants of the metallated protein. The results agree ...
The crude ethyl acetate extract of the leaves of Cornus macrophylla showed antibacterial activity against Pseudomonas aeruginosa, a leading cause of illness in immunocompromised individuals. Bioactivity-guided separation led to the isolation of kaempferol 3-O-α-L-rhamnopyranoside (afzelin). The structure was determined based on evaluation of its spectroscopic (UV, MS, and NMR) data. The minimum inhibitory concentration (MIC) of afzelin against Pseudomonas aeruginosa was found to be 31 µg/mL. In addition, the results indicated that a hydroxyl group at C3 of the C-ring of the flavone skeleton and the rhamnose group may act as a negative factor and an enhancing factor, respectively, in the antibacterial activities of afzelin.
TY - JOUR. T1 - Lipopolysaccharide-free Escherichia coli OmpF and Pseudomonas aeruginosa protein P porins are functionally active in lipid bilayer membranes. AU - Parr, T. R.. AU - Poole, K.. AU - Crockford, G. W.K.. AU - Hancock, R. E.W.. PY - 1986/1/1. Y1 - 1986/1/1. UR - http://www.scopus.com/inward/record.url?scp=0022466549&partnerID=8YFLogxK. U2 - 10.1128/jb.165.2.523-526.1986. DO - 10.1128/jb.165.2.523-526.1986. M3 - Article. C2 - 3003028. AN - SCOPUS:0022466549. VL - 165. SP - 523. EP - 526. JO - Journal of bacteriology. JF - Journal of bacteriology. SN - 0021-9193. IS - 2. ER - ...
BACKGROUND Pseudomonas aeruginosa bacteremia (PAB) is associated with high mortality and morbidity rates, but the outcome for patients with PAB has not been recently well evaluated. METHODS Between 1997 and 1999, all episodes of PAB at the Hôtel-Dieu de France University Hospital, Lebanon, were analyzed to evaluate the outcome for patients with PAB. RESULTS Fifty-five episodes of PAB in 53 patients (26 episodes in men and 29 in women) were analyzed. The mean age of the patients in the cohort was 60.7 years (range: 18-89 years). The mean time between the onset of hospitalization and the first episode of PAB was 21 days (range: 0-77 days). Most of the tested isolates showed favorable in vitro susceptibility to ceftazidime (85%), amikacin (77%) and imipenem (67%). The overall in-hospital cumulative survival was 89% at one week and 49% at 2 months. Among the variables analyzed, four were statistically associated with a higher mortality rate: prior use of antimicrobials (85% vs 54%), use of systemic
Pseudomonas aeruginosa is a nosocomial pathogen that causes severe infections in immunocompromised patients. Biofilm plays a significant role in the resistance of this bacterium and complicates the treatment of its infections. In this study, the effect of lyticase and β-glucosidase enzymes on the degradation of biofilms of P. aeruginosa strains isolated from cystic fibrosis and burn wound infections were assessed. Moreover, the decrease of ceftazidime minimum biofilm eliminating concentrations (MBEC) after enzymatic treatment was evaluated. This study demonstrated the effectiveness of both enzymes in degrading the biofilms of P. aeruginosa. In contrast to the lyticase enzyme, β-glucosidase reduced the ceftazidime MBECs significantly (P | 0.05). Both enzymes had no cytotoxic effect on the A-549 human lung carcinoma epithelial cell lines and A-431 human epidermoid carcinoma cell lines. Considering the characteristics of the β-glucosidase enzyme, which includes the notable degradation of P. aeruginosa
Pseudomonas aeruginosa is a nosocomial pathogen that causes severe infections in immunocompromised patients. Biofilm plays a significant role in the resistance of this bacterium and complicates the treatment of its infections. In this study, the effect of lyticase and β-glucosidase enzymes on the degradation of biofilms of P. aeruginosa strains isolated from cystic fibrosis and burn wound infections were assessed. Moreover, the decrease of ceftazidime minimum biofilm eliminating concentrations (MBEC) after enzymatic treatment was evaluated. This study demonstrated the effectiveness of both enzymes in degrading the biofilms of P. aeruginosa. In contrast to the lyticase enzyme, β-glucosidase reduced the ceftazidime MBECs significantly (P | 0.05). Both enzymes had no cytotoxic effect on the A-549 human lung carcinoma epithelial cell lines and A-431 human epidermoid carcinoma cell lines. Considering the characteristics of the β-glucosidase enzyme, which includes the notable degradation of P. aeruginosa
The structural gene (lipA) coding for the extracellular lipase of Pseudomonas aeruginosa PAO1 has been cloned on plasmid pSW118. Nucleotide sequence analysis revealed a gene of 936 bp. lipA codes for a proenzyme of 311 amino acids including a leader sequence of 26 amino acids. The mature protein was predicted to have a M r of 30134, an isoelectric point of 5.6, and a consensus sequence (IGHSHGG) typical of lipases. Furthermore it is highly homologous (>60%) to other lipases from various pseudomonads. The lipA gene failed to hybridize detectably with genomic DNA from other Pseudomonas species except P. alcaligenes, even under relaxed stringency. Located 220 bp downstream of the lipA gene, is an open reading frame (ORF2, lipH) which encodes a hydrophilic protein (283 amino acids; M r 33587) that shows some homology to the limA gene product of P. cepacia. In complementation tests of lipase-defective mutants, lipH was shown to be necessary for expression of active extracellular lipase in P. aeruginosa
BACKGROUND: Extensively drug-resistant (XDR) Pseudomonas aeruginosa and Acinetobacter baumannii are a threat to hospitalized patients. We evaluated the effects of antimicrobial combinations on XDR P. aeruginosa and A. baumannii isolates.. METHODS: P. aeruginosa and A. baumannii isolates, which were resistant to all antibiotics except colistin (CL), were collected from eight hospitals in Korea. Genes encoding metallo-β-lactamases (MBLs) and OXA carbapenemases were detected by PCR in eight P. aeruginosa and 30 A. baumannii isolates. In vitro synergy of antimicrobial combinations was tested by using the checkerboard method.. RESULTS: Minimum inhibitory concentrations of β-lactams, aminoglycosides, and fluoroquinolones were very high, while that of CL was low for majority of XDR P. aeruginosa and A. baumannii isolates. Antimicrobial combinations including Imipenem (IPM)-CL, ceftazidime (CAZ)-CL, and rifampin (RIF)-CL exerted only additive/indifferent effects on majority of XDR P. aeruginosa ...
TY - JOUR. T1 - Stimulus-permeability coupling in rat pulmonary macrophages challenged by Pseudomonas aeruginosa - An X-ray microanalysis study. AU - Smith, Nancy K.R.. AU - Lewinski, Andrzej K.. AU - Mangos, John A.. AU - Lee Boyd, R.. PY - 1985/5/1. Y1 - 1985/5/1. N2 - Electron probe X-ray microanalysis (XRMA) of freeze-dried ultrathin sections provides the capability of measuring intracellular elemental content. This methodology was used to investigate the stimulus-permeability coupling responses associated with phagocytosis of Pseudomonas aeruginosa by cultured pulmonary alveolar macrophages (PAMs) of rats. PAMs were challenged with P. aeruginosa suspended in Geys buffer at a bacteria to PAM ratio of 50:1 for 1 h at 37° C. A 1-mm3 pellet of the unchallenged control PAMs, challenged PAMs and P. aeruginosa alone was quench-frozen in nitrogen-cooled, liquid propane, and 0.1-μm cryosections were cut at -100° C. X-ray spectra were collected for nucleus and cytoplasm of 39 control PAMs, 36 ...
Fluorescently labelled latex microbeads were used to study the interaction of particles with Pseudomonas aeruginosa biofilms in a continuous flow annular reactor. Beads were readily distinguished and enumerated in both intact and disaggregated biofilm samples. The fraction of beads that attached to biofilm during a 24 h period ranged from 0.001 to 0.01 and was proportional to biofilm cell carbon and to the standard deviation of biofilm thickness. Microbeads added to biofilm of steady state thickness (30 μm) were observed to be located throughout the entire biofilm depth in 24 h. Many of the microbeads that attached to biofilm shortly after bacterial inoculation (thickness of 2 μm) remained near the substratum as cells grew past and covered them. Microbeads were observed near the biofilm-substratum interface for up to 5 days after bead addition. Beads formed aggregates on biofilms, but not in bulk water. Beads captured by biofilm remained in the reactor system longer than beads that never ...
In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedis biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Five MDR clinical lab isolates (LS 22, LS 23, LS 38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated. Control and treated group were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed 60% alteration in sensitivity of tested antimicrobials in MDR isolates of P. aeruginosa after biofield treatment. MIC results showed an alteration in 42.85% tested antimicrobials out of twenty eight after biofield treatment in five isolates
Rhamnolipid biosurfactants were continuously produced with Pseudomonas aeruginosa on the pilot plant scale. Production and downstream processing elaborated on the laboratory scale were adapted to the larger scale. Differences in performance resulting from the scale-up are discussed. A biosurfactant concentration of approximately 2.25 g liter-1 was achieved. The biosurfactant yield on glucose was 77 mg g-1 h-1, and the productivity was 147 mg liter-1 h-1, corresponding to a daily production of 80 g of biosurfactant. The first enrichment step consisted of an adsorption chromatography which was followed by an anion-exchange chromatography. The resulting product was 90% pure, and the overall recovery of active material was above 60% with the downstream processing used. ...
Pseudomonas aeruginosa is an opportunistic pathogen associated with life-threatening nosocomial and community-acquired infections. Antibiotic resistance is an immediate threat to public health and demands an urgent action to discovering new antimicrobial agents. One of the best alternatives for pre-clinical tests with animal models is the greater wax moth Galleria mellonella. Here, we evaluated the antipseudomonal activity of silver nanoparticles (AgNPs) against P. aeruginosa strain UCBPP-PA14 using G. mellonella larvae. The AgNPs were synthesized through a non-toxic biogenic process involving microorganism fermentation. The effect of AgNPs was assessed through characterization and quantification of the hemocytic response, nodulation and phenoloxidase cascade. On average, 80% of the larvae infected with P. aeruginosa and prophylactically treated with nanoparticles survived. Both the specific and total larvae hemocyte counts were restored in the treated group. In addition, the nodulation process and the
Ceftazidime treatment of chronic Pseudomonas aeruginosa respiratory tract infection in cystic fibrosis.: Two open randomized cross-over studies were undertaken
Nitrate respiration is a widespread mode of anaerobic energy generation used by many bacterial pathogens, and the respiratory nitrate reductase, Nar, has long been known to reduce chlorate to the toxic oxidizing agent chlorite. Here, we demonstrate the antibacterial activity of chlorate against Pseudomonas aeruginosa, a representative pathogen that can inhabit hypoxic or anoxic host microenvironments during infection. Aerobically grown P. aeruginosa cells are tobramycin sensitive but chlorate tolerant. In the absence of oxygen or an alternative electron acceptor, cells are tobramycin tolerant but chlorate sensitive via Nar-dependent reduction. The fact that chlorite, the product of chlorate reduction, is not detected in culture supernatants suggests that it may react rapidly and be retained intracellularly. Tobramycin and chlorate target distinct populations within metabolically stratified aggregate biofilms; tobramycin kills cells on the oxic periphery, whereas chlorate kills hypoxic and anoxic ...
Barsoukov E. and J.R. Macdonald (eds). 2005. Impedance Spectroscopy: Theory, Experiment and Applications, 2nded. John Wiley & Sons, Inc., Hoboken, NJ, USA.. Ben-Yoav H., A. Freemanb, M. Sternheimc and Y. Shacham-Dia-manda. 2011. An electrochemical impedance model for integrated bacterial biofilms. Electrochim. Acta. 56:7780-7786.. Bjarnsholt T., K. Kirketerp-Møller, P.Ø. Jensen, K.G. Madsen, R. Phipps, K. Krogfelt, N. Høibyand and M. Givskov. 2008. Why chronic wounds will not heal: a novel hypothesis. Wound Rep. Reg. 16:2-10.. Dominguez-Benetton X., S. Sevda, K. Vanbroekhovena. and D. Panta. 2012. The accurate use of impedance analysis for thestudy of microbial electrochemical systems. Chem. Soc. Rev. 41:7228-7246.. Flemming H., J. Wingender and U. Szewczyk (eds). 2008. Biofilm Highlights. Springer Series on Biofilm Vol. 5. Springer-Verlag, Berlin, Heidelberg.. Ge Y., T. Deng and X. Zheng. 2008. Dynamic monitoring of changes in endothelial cell-substrate adhesiveness during leukocyte adhesion ...
The gram-negative bacterium Pseudomonas aeruginosa catalyzes the conversion of ricinoleic acid into a novel trihydroxy fatty acid, 7, 10, 12-trihydroxy-8(E)-octadecenoic acid (TOD), that has a potent antifungal activity against important crop pathogens, including Magnaporthe grisea the causative agent of rice blast disease. Natural crop-protecting agents such as TOD offer several advantages over synthetic agents, including improved ecological compatibility and environmental safety. Unfortunately, because many naturally occurring crop-protecting agents are produced only in trace amounts, it has been difficult to isolate large enough quantities of these antimicrobial agents to be economically feasible. Thus, a bacterium such as P. aeruginosa that is genetically amenable and produces an antifungal agent is ideal for genetic manipulation to achieve improved TOD production. The long-term goal of this research is to develop efficient processes for improving production of TOD from P. aeruginosa to ...
This is the largest US claims database study of healthcare costs and outcomes for ICU patients with a diagnosis of S. aureus or P. aeruginosa pneumonia. Our findings highlight the comprehensive economic consequences attributed to S. aureus and P. aeruginosa pneumonia and can permit policy makers, payers, and healthcare providers to assess the effect of prevention or therapeutic efforts on the cost and morbidity of these ICU infections.. In our study, ICU patients with pneumonia had substantially higher healthcare costs during the index admission: , $213,000 for P. aeruginosa pneumonia and , $146,000 for with S. aureus pneumonia versus ,$33,000 for patients without pneumonia. Increased utilization continued after index hospitalization discharge, with total healthcare costs through 90 days post discharge of , $17,000 for patients with S. aureus pneumonia and , $22,000 for patients with P. aeruginosa pneumonia versus , $10,000 for patients without pneumonia. Patients with S. aureus or P. aeruginosa ...
IL-1β is a potent proinflammatory cytokine produced in response to Pseudomonas aeruginosa infection. While it is appreciated that IL-1β is a critical modulator of the host response to P. aeruginosa infection, the host and bacterial determinants of IL-1β production during both acute and chronic infections are less well understood. This thesis is focused on the determinants of the IL-1β response to P. aeruginosa. The effects of bacterial phenotypic changes during chronic infection, such as loss of flagellar motility, on the IL-1β response are poorly understood. Bacterial flagellar motility is a fundamental mechanism, which enables bacterial association with leukocytes. Therefore, we hypothesized that loss of bacterial flagellar motility would facilitate evasion of contact-dependent inflammasome activation and IL-1β production. In support of this hypothesis, we demonstrate that bacterial flagellar motility contributes to inflammasome activation and correspondingly, nonmotile P. aeruginosa ...
Exoenzyme S (ExoS) is a mono-ADP-ribosyltransferase secreted by the opportunistic pathogen Pseudomonas aeruginosa. ExoS requires a eukaryotic factor, the 14-3-3 protein, for enzymatic activity. Here, two aspects of the activation of the ADP-ribosyltransferase activity of ExoS by 14-3-3 proteins are …
Most microbial pathogens have a metabolic iron requirement, necessitating the acquisition of this nutrient in the host. In response to pathogen invasion, the human host limits iron availability. Although canonical examples of nutritional immunity are host strategies that limit pathogen access to Fe(III), little is known about how the host restricts access to another biologically relevant oxidation state of this metal, Fe(II). This redox species is prevalent at certain infection sites and is utilized by bacteria during chronic infection, suggesting that Fe(II) withholding by the host may be an effective but unrecognized form of nutritional immunity. Here, we report that human calprotectin (CP; S100A8/S100A9 or MRP8/MRP14 heterooligomer) inhibits iron uptake and induces an iron starvation response in Pseudomonas aeruginosa cells by sequestering Fe(II) at its unusual His6 site. Moreover, under aerobic conditions in which the Fe(III) oxidation state is favored, Fe(II) withholding by CP was enabled ...
Conclusion: Positive microbiology and genomic DNA typing results proved that the contaminated trypan blue solutions were the source of infection in this outbreak. Postoperative endophthalmitis caused by Pseudomonas aeruginosa is often associated with a poor visual prognosis despite prompt treatment with intravitreal antibiotics. PMID: 31660104 [PubMed]...
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The ELITE trial shows that treatment with TIS for 28 days is effective for treating early P aeruginosa infection, and extending TIS treatment to 56 days does not provide notable additional improvements. Over 90% of randomised patients in the 28-day and 56-day TIS groups had negative cultures for P aeruginosa 1 month after the end of treatment, and the majority of these patients remained free from infection for up to 27 months. The results were similar in sputum and non-sputum producers and were unaffected by the patients baseline characteristics (age, lung function and first versus recurrent infection).. Antibiotics, either alone or in combination, have been shown to be effective in the treatment of early P aeruginosa infection.3-6 11 14 However, to date there have been no reports of comparative studies assessing different treatment regimens and only a few controlled studies have been performed. In an open controlled trial, inhaled colistin and oral ciprofloxacin twice daily for 3 weeks was ...
OBJECTIVE: Pseudomonas aeruginosa is one of the principal causes of bacterial infections in healthcare and it represents a critical problem worldwide. Infections are becoming more difficult to treat because this bacterium is resistant to many commonly used antibiotics, including aminoglycosides, cephalosporins, fluoroquinolones and carbapenems. Drug resistance is associated with worse clinical outcomes: it facilitates prolonged hospitalization, multiple morbidities and increased health costs. The aim of this work was to evaluate the epidemiology of Pseudomonas aeruginosa during the years 2015-2017 in G. Martino University Hospital of Messina (Messina, Italy ...
TY - JOUR. T1 - Iron-mediated regulation of alkaline proteinase production in Pseudomonas aeruginosa. AU - Shigematsu, Takashi. AU - Fukushima, Jun. AU - Oyama, Masatsune. AU - Tsuda, Masataka. AU - Kawamoto, Susumu. AU - Okuda, Kenji. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2001. Y1 - 2001. N2 - We analyzed the regulation by iron of alkaline proteinase (AP) production in Pseudomonas aeruginosa. Extracellular AP production was detected from the mid-logarithmic to the stationary phase by an antibody-based assay system, and was strongly repressed by iron in the medium. This repression was shown by Northern hybridization and primer extension to occur at the level of transcription. The primer extension analysis revealed that the start point of transcription of AP gene was the nucleotide position -84 from the start point of translation. Furthermore, we investigated whether this transcriptional repression involved PvdS protein. Using the mutant strain of pvdS, the ...
However, some bacteria in which cyclic di-GMP has been studied lack cyclic di-GMP-I riboswitches, e.g. Pseudomonas aeruginosa. ...
Thi Bach Nguyen H, Romero AD, Amman F, Sorger-Domenigg T, Tata M, Sonnleitner E, Bläsi U (Oct 2018). "Pseudomonas aeruginosa". ... E.g., in the opportunistic human pathogen Pseudomonas aeruginosa the sRNA ReaL translationally silences rpoS mRNA. RpoS ...
... (EC 3.4.24.26, Pseudomonas elastase, Pseudomonas aeruginosa neutral metalloproteinase) is an enzyme. This enzyme ... Nishino N, Powers JC (April 1980). "Pseudomonas aeruginosa elastase. Development of a new substrate, inhibitors, and an ... Bever RA, Iglewski BH (September 1988). "Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa ... "Pseudomonas aeruginosa elastase: affinity chromatography and some properties as a metallo-neutral proteinase". Agric. Biol. ...
and Pseudomonas aeruginosa. Dentists are at the top of the working groups who have a high risk of exposure to COVID-19. SARS- ...
and Pseudomonas aeruginosa. Exposure to higher doses of radiation is associated with systemic anaerobic infections due to gram ... including Pseudomonas aeruginosa and certain Enterobacteriaceae that generally are resistant to most third-generation ... and organisms that are acquired from the environment such as Pseudomonas spp. Organisms causing sepsis following exposure to ... Ciprofloxacin is effective against Gram-negative organisms (including Pseudomonas species) but has poor coverage for Gram- ...
and Pseudomonas aeruginosa". The Journal of Antibiotics. 60 (5): 335-8. doi:10.1038/ja.2007.45. PMID 17551215. Eja ME, Asikong ...
O'Toole, G. A.; Kolter, R. (October 1998). "Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm ... Friedman, Lisa; Kolter, Roberto (2004). "Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms". Molecular ... O'Toole, GA; Flagellar, Kolter R. (1998). "Pseudomonas aeruginosa biofilm development". Mol Microbiol. 30 (2): 295-304. doi: ... of Pseudomonas aeruginosa". Journal of Bacteriology. 189 (14): 5383-5386. doi:10.1128/JB.00137-07. ISSN 0021-9193. PMC 1951888 ...
Pseudomonas aeruginosa alpha toxin. This disambiguation page lists articles associated with the title Alpha toxin. If an ...
It can be caused by infection, particularly from Pseudomonas species, such as Pseudomonas aeruginosa, Clostridium species, ... Bodey, G. P.; Bolivar, R.; Fainstein, V.; Jadeja, L. (1983). "Infections Caused by Pseudomonas aeruginosa". Clinical Infectious ... and ecthyma gangrenosum in an immunocompromised host with pseudomonas septicemia". American Journal of Ophthalmology. 137 (1): ...
Walker GC, Nicholas DJ (1961). "Nitrite reductase from Pseudomonas aeruginosa". Biochim. Biophys. Acta. 49 (2): 350-360. doi: ... Singh J (1974). "Cytochrome oxidase from Pseudomonas aeruginosa. III. Reduction of hydroxylamine". Biochim. Biophys. Acta. 333 ... "In vivo studies disprove an obligatory role of azurin in denitrification in Pseudomonas aeruginosa and show that azu expression ...
... was shown to be ineffective in vitro against Enterobacter spp., Pseudomonas aeruginosa, and Xanthomonas maltophilia ...
Erwinia herbicola and Pseudomonas aeruginosa are known to have homologues for cyclohexadienyl dehydratase. Of the plants with ... Zhao GS, Xia TH, Fischer RS, Jensen RA (February 1992). "Cyclohexadienyl dehydratase from Pseudomonas aeruginosa. Molecular ... "The crystal structure of cyclohexadienyl dehydratase precursor from Pseudomonas aeruginosa PA01". doi:10.2210/pdb3kbr/pdb. Cite ...
Exotoxin A of Pseudomonas aeruginosa; Pertussis toxin of B. Pertussis; C3 toxin of C. botulinum; and Diphtheria toxin of ...
"Hydroxylamine reductase from Pseudomonas aeruginosa". Biochim. Biophys. Acta. 49 (2): 361-368. doi:10.1016/0006-3002(61)90135-4 ...
Pseudomonas Aeruginosa species of bacteria are the most common causes of Green Nail Syndrome. Nail scrapings have identified ... It is most commonly caused by the bacteria "Pseudomonas Aeruginosa", which thrives in moist conditions. Green Nail Syndrome is ... Green nail syndrome, is a paronychial infection caused due to Pseudomonas aeruginosa that can develop in individuals whose ... "Pseudomonas aeruginosa Infections: Clinical Presentation". eMedicine. Retrieved 1 February 2014. James, William; Berger, ...
Fewson CA, Nicholas DJ (May 1961). "Nitrate reductase from Pseudomonas aeruginosa". Biochimica et Biophysica Acta. 49 (2): 335- ...
... due to a Pseudomonas aeruginosa infection causing a green nail syndrome or (2) the result of copper in tap water. Pseudomonas ... ISBN 0-7216-2921-0. Balcht, Aldona; Smith, Raymond (1994). Pseudomonas Aeruginosa: Infections and Treatment. Informa Health ... aeruginosa is a common bacterium that can cause disease in animals, including humans. It is found in soil, water, skin flora, ...
Ramakrishnan T; Campbell JJR (1955). "Gluconic dehydrogenase of Pseudomonas aeruginosa". Biochim. Biophys. Acta. 17 (1): 122- ...
Furthermore, bacteriocins active against E. coli, Salmonella and Pseudomonas aeruginosa have been produced in planta with the ... Michel-Briand Y, Baysse C (2002). "The pyocins of Pseudomonas aeruginosa". Biochimie. 84 (5-6): 499-510. doi:10.1016/s0300-9084 ... Most well studied are the tailocins of Pseudomonas aeruginosa. They can be further subdivided into R-type and F-type pyocins. ... "Plant-expressed pyocins for control of Pseudomonas aeruginosa". PLOS ONE. 12 (10): e0185782. Bibcode:2017PLoSO..1285782P. doi: ...
... and Pseudomonas aeruginosa". Frontiers in Immunology. 6: 595. doi:10.3389/fimmu.2015.00595. PMC 4655328. PMID 26635809. ... The enzymes involved in Lipid A synthesis are conserved among Pseudomonas aeruginosa, Escherichia coli, Bordetella ... "Lipopolysaccharide biosynthesis in Pseudomonas aeruginosa". Innate Immunity. 15 (5): 261-312. doi:10.1177/1753425909106436. ...
Guan R, Ho MC, Almo SC, Schramm VL (February 2011). "Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and ... methylthioadenosine in Pseudomonas aeruginosa involves deamination to S-methyl-5'-thioinosine (EC 3.5.4.31, S-methyl-5'- ...
"Tolerance and Resistance of Pseudomonas aeruginosa Biofilms to Antimicrobial Agents-How P. aeruginosa Can Escape Antibiotics". ... Secreted by Pseudomonas aeruginosa, this compound induces cyclo heteromorphic cells in several species of bacteria and the ... Moreover, Pseudomonas aeruginosa biofilms can impede the slithering motility of C. elegans, termed as 'quagmire phenotype', ... For instance, the biofilm form of Pseudomonas aeruginosa has no greater resistance to antimicrobials than do stationary-phase ...
IPL is a PchB enzyme originating from the pchB gene in Pseudomonas aeruginosa. Isochorismate lyase, or PchB, is part of the ... Olucha J, Ouellette AN, Luo Q, Lamb AL (August 2011). "pH Dependence of catalysis by Pseudomonas aeruginosa isochorismate- ... "BRENDA - Information on EC 4.2.99.21 - isochorismate lyase and Organism(s) Pseudomonas aeruginosa". www.brenda-enzymes.info. ... Derived from the pchB gene of Pseudomonas aeruginosa, other names for isochorismate lyase include: salicylate biosynthesis ...
Pseudomonas aeruginosa Pseudomonas pyocyanea Salmonella spp. Selenomonas sputigena Shigella sonnei Staphylococcus aerogenes ... Rada B, Leto TL (2009). "Redox warfare between airway epithelial cells and Pseudomonas: Dual oxidase versus pyocyanin". Immunol ... Rada B, Leto TL (2009). "Redox warfare between airway epithelial cells and Pseudomonas: Dual oxidase versus pyocyanin". Immunol ...
Comolli, J. C.; Hauser, A. R.; Waite, L.; Whitchurch, C. B.; Mattick, J. S.; Engel, J. N. (July 1999). "Pseudomonas aeruginosa ... It has been observed in many bacterial species, but is most well studied in Pseudomonas aeruginosa, Neisseria gonorrhoeae and ... Burrows, Lori L. (2012). "Pseudomonas aeruginosa twitching motility: type IV pili in action". Annual Review of Microbiology. 66 ... Parsek, Matthew R.; Tolker-Nielsen, Tim (December 2008). "Pattern formation in Pseudomonas aeruginosa biofilms". Current ...
Pseudomonas aeruginosa, Salmonella enterica, E.coli. Firmacutes [sic] (gram positive, subsequently corrected to Firmicutes) ... The class Schizomycetes was then emended by Walter Migula (along with the coinage of the genus Pseudomonas in 1894) and others ...
In case of people having a high risk of being infected with multiple drug resistant organisms such as Pseudomonas aeruginosa, ... Other commonly implicated bacteria include Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella ... The bacteria Streptococcus pneumoniae, Escherichia coli, Proteus spp., Pseudomonas aeruginosa, Staphylococcus aureus, ...
Morita Y, Tomida J, Kawamura Y (2014). "Responses of Pseudomonas aeruginosa to antimicrobials". Front Microbiol. 4: 422. doi: ... Imipenem, doripenem, and meropenem also exhibit good activity against most strains of Pseudomonas aeruginosa and Acinetobacter ... Infections caused by the non-fermenting gram-negative bacteria Pseudomonas aeruginosa and Acinetobacter baumanni are most ... Pseudomonas produces an inducible broad spectrum beta lactamase, AmpC, that is produced in response to beta lactam exposure. ...
Pseudomonas aeruginosa strain SQ1, Klebsiella sp. strain ABR11, Klebsiella oxytoca TauN1, Agrobacterium sp. strain ABR2, and ... The sulfoglycolytic Entner-Doudoroff (sulfo-ED) pathway, first identified in Pseudomonas putida SQ1, involves the degradation ... "Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1". Proceedings of the National Academy of ...
Sonnleitner, E; Romeo, A; Bläsi, U (Apr 2012). "Small regulatory RNAs in Pseudomonas aeruginosa". RNA Biology. 9 (4): 364-71. ...
Mennink-Kersten MA, Ruegebrink D, Verweij PE (2008). "Pseudomonas aeruginosa as a cause of 1,3-β-D-glucan assay reactivity". ... Pseudomonas aeruginosa, and Alcaligenes faecalis, which also produce (1→3)β-D-glucan.[25] This test can aid in the detection of ...
"Quorum-sensing-regulated virulence factors in Pseudomonas aeruginosa are toxic to Lucilia sericata maggots". Microbiology 156 ( ... belatung mungkin merupakan metode biaya-efektif untuk mengelola bakteri MRSA infection.Other seperti Pseudomonas aeruginosa, E. ... coli atau Proteus spek tidak diserang oleh belatung dan dalam hal Pseudomonas bahkan belatung berada di danger.Consequently, ...
S. agalactiae) pri starejših osebah z diabetesom, Haemophilus influenzae pri otrocih in Pseudomonas aeruginosa pri tistih z ...
Pseudomonas (11%), the fungal pathogen Candida albicans (9%), and Enterococcus (7%) among others.[6][24][25] Urinary tract ... Pseudomonas aeruginosa *Pseudomonas infection. *Moraxella catarrhalis. *Acinetobacter baumannii. Xanthomonadaceae. * ...
... biofilms are present in the chronic Pseudomonas aeruginosa and Burkholderia cenocepacia infections characteristic of cystic ...
Pseudomonas, and Alcaligenes). Bacteria grown in liquid cultures often form colloidal suspensions.[4][5] ... Pseudomonas aeruginosa *Cetrimide agar. *Salmonella *XLT agar. *DCA agar. *Salmonella/Shigella *XLD agar ...
... for antimicrobial susceptibility testing of Pseudomonas aeruginosa". Journal of Clinical Microbiology 30 (10): 2709-2713. PMC ... "Comparison of Etest with agar-dilution for testing the susceptibility of P. aeruginosa and other MDR bacteria to Colistin." JAC ... "Breakpoints for Predicting P.aeruginosa Susceptibility to Inhaled Tobramycin in Cystic Fibrosis Patients: Use of High-Range ... Pseudomonas, Staphylococcus, and Enterococcus species and fastidious bacteria, such as anaerobes, N. gonorrhoeae, S. pneumoniae ...
Pseudomonas aeruginosa ‎ (→‎Antibiotic resistance) *01:39, 6 March 2015 (diff , hist) . . (+2,745)‎ . . Pseudomonas aeruginosa ...
... sledita okužba z bakterijama Haemophilus influenzae in Pseudomonas aeruginosa, medtem ko pri starejših adolescentih in odraslih ...
Pseudomonas extremorientalis. *Pseudomonas fluorescens. *Pseudomonas jinjuensis. *Pseudomonas koreensis. *Pseudomonas ... Microcystis aeruginosa. *Mikobakteria. *Mycobacterium avium complex. *Mycobacterium leprae. *Mycobacterium tuberculosis. * ...
B96.5) Pseudomonas (aeruginosa)(mallei)(pseudomallei) as the cause of diseases classified to other chapters ...
bakterie Pseudomonas aeruginosa (rezistentní proti řadě antibiotik), nebezpečná pro pacienty trpící cystickou fibrózou, využívá ...
Pseudomonas aeruginosa *Pseudomonas infection. *Moraxella catarrhalis. *Acinetobacter baumannii. Xanthomonadaceae. * ...
Pseudomonas aeruginosa infection. *Small bowel bacterial overgrowth syndrome. *Strongyloidiasis. *Tropical sprue. *Weight gain ...
Pseudomonas aeruginosa. *Candida onddoa. *HACEK taldeko mikroorganimoak (Haemophilus parainfluenzae, Actinobacillus, ...
Vibrio cholerae and Pseudomonas aeruginosa.[19][20] These were identified when mutations in the Hcp and VrgG genes in Vibrio ... "A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus". Science. 312 (5779): 1526-30. Bibcode: ... to the Pseudomonas fluorescens cell adhesion protein LapA of 520 kDa.[7] The best characterized are the RTX toxins and the ... "Structural features of the Pseudomonas fluorescens biofilm adhesin LapA required for LapG-dependent cleavage, biofilm ...
... such as Pseudomonas aeruginosa.[9][10] Side effects[edit]. The most common adverse effects are diarrhea (4.8%), nausea and ... The spectrum of action includes many Gram-positive and Gram-negative bacteria (including Pseudomonas) and anaerobic bacteria. ...
... საბოლოოდ დომინირებს Pseudomonas aeruginosa (და ზოგჯერ Burkholderia cepacia). 18 წლის ასაკისთვის პაციენტების 80% P. aeruginosa-ს ... მწვანე = Pseudomonas aeruginosa. ყავისფერი = Staphylococcus aureus. ლურჯი = Haemophilus influenzae. წითელი =Burkholderia ... Pseudomonas aeruginosa კოლონიზდება კისტური ფიბროზით დაავადებული ინდივიდების ფილტვებში, რაც იწვევს იმუნური პასუხის დაქვეითებას, ... "Inhaled aztreonam lysine for chronic airway Pseudomonas aeruginosa in cystic fibrosis". Am. J. Respir. Crit. Care Med. 178 (9 ...
"Spaceflight Promotes Biofilm Formation by Pseudomonas aeruginosa". PLOS ONE. 8 (4): e6237. Bibcode:2013PLoSO...862437K. doi: ...
... and Pseudomonas aeruginosa.[7] A person affected with tracheitis may start with symptoms that suggest an upper respiratory ...
Pseudomonas aeruginosa (Pseudomonas infection) · Moraxella catarrhalis · Acinetobacter baumannii. Xanthomonadales. ...
Pseudomonas aeruginosa *Cetrimide agar. *Salmonella *XLT agar. *DCA agar. *Salmonella/Shigella *XLD agar ...
Pseudomonas aeruginosa. *Pseudomonas extremorientalis. *Pseudomonas fluorescens. *Pseudomonas jinjuensis. *Pseudomonas ...
Proteobacteria, contains most of the "commonly known" species, such as Escherichia coli and Pseudomonas aeruginosa. ... is Pseudomonas whose etymology ironically matched its taxonomy, namely "false unit".[12] ...
... intolerance Obstructed bowel Ovarian cancer Polycystic liver disease Pregnancy Premenstrual syndrome Pseudomonas aeruginosa ...
"Di-rhamnolipid from Pseudomonas aeruginosa displays differential effects on human keratinocyte and fibroblast cultures". J. ...
Pseudomonas aeruginosa ja Salmonella typhosa, kasvu.[25]. Hariliku sibula petroleum ether extract inhibeeris bakterite ...
Comparison of denitrification by Pseudomonas stutzeri, Pseudomonas aeruginosa, and Paracoccus denitrificans. Appl Environ ... Contoh bakteri yang mampu melakukan metabolisme ini adalah Pseudomonas stutzeri, Pseudomonas aeruginosa, and Paracoccus ... Pseudomonas cocovenenans), menghasilkan asam bongkrek, terdapat pada tempe bongkrek[51]. *Leuconostoc mesenteroides, penyebab ... dan Pseudomonas fluorescens sebagai Agens Pengendali Penyakit Busuk Lunak Bakteri (Erwinia carotovora) pada Anggrek ...
Pseudomonas aeruginosa is a type of germ that can cause infections in humans, mostly in hospital patients. It can cause ... Pseudomonas aeruginosa lives in the environment and can be spread to people in healthcare settings when they are exposed to ... CDC tracks Pseudomonas aeruginosa and the infections this germ can cause, including antibiotic-resistant infections. ... Pseudomonas aeruginosa infections are generally treated with antibiotics. Unfortunately, in people exposed to healthcare ...
Since July 2020, CDC has received eight reports of highly antibiotic-resistant Pseudomonas aeruginosa infections in patients ... Testing resources for Pseudomonas aeruginosa are available through the Antibiotic Resistance Laboratory Network which can be ... CDC will continue to monitor reports of Pseudomonas aeruginosa infection linked to invasive procedures in Mexico. ... Highly antibiotic resistant Pseudomonas aeruginosa continue to be reported among travelers with infections who underwent ...
Carbenicillin resistance in Pseudomonas aeruginosa.. Br Med J 1969; 4 doi: https://doi.org/10.1136/bmj.4.5677.234-a (Published ...
Pseudomonas aeruginosa *↑ Balcht, Aldona & Smith, Raymond. Pseudomonas Aeruginosa: Infections and Treatment. Informa Health ... Pseudomonas aeruginosa (o bacil piociànic, bacil del pus blau, bacil del pus verd) és un bacteri comú que causa malalties en ... Rahme LG, Tan MW, Le L, et al. «Use of model plant hosts to identify Pseudomonas aeruginosa virulence factors». Proc. Natl. ... La mel de grau mèdic pot reduir la colonització de molts patògens incloent Pseudomonas aeruginosa.[14] La profilaxi probiòtica ...
... aeruginosa causes infection is through quorum sensing; a type of cell signalling pathway that initiates the production of ... Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors. *Cell-to-cell signaling and Pseudomonas ... Pseudomonas aeruginosa is a human pathogen which can cause life-threatening illness, especially in people who have a depleted ... By using furanones to prevent quorum sensing and reduce biofilm formation, severe Pseudomonas aeruginosa infections could be ...
Pseudomonas is a gram-negative rod that belongs to the family Pseudomonadaceae. More than half of all clinical isolates produce ... encoded search term (Pseudomonas%20aeruginosa%20Infections) and Pseudomonas aeruginosa Infections What to Read Next on Medscape ... Pseudomonas aeruginosa Infections Medication. Updated: Dec 05, 2016 * Author: Marcus Friedrich, MD, MBA, FACP; Chief Editor: ... Infections with Pseudomonas aeruginosa in Charcot arthropathy of the foot. Foot Ankle Int. 2013 Feb. 34(2):234-7. [Medline]. ...
Defining the Pseudomonas aeruginosa SOS response and its role in the global response to the antibiotic ciprofloxacin. Cirz RT, ... Cystic fibrosis sputum supports growth and cues key aspects of Pseudomonas aeruginosa physiology. Palmer KL, et al. J Bacteriol ... Effect of anaerobiosis and nitrate on gene expression in Pseudomonas aeruginosa. Filiatrault MJ, et al. Infect Immun, 2005 Jun ... Pseudomonas aeruginosa PAO1 (strain: PAO1) Lineage. Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; ...
Directed Evolution of Pseudomonas aeruginosa Lipase A Petroleum has long been a precious resource throughout history. Petroleum ... Pseudomonas aeruginosa Essay. 2797 Words , 12 Pages. Pseudomonas aeruginosa Pseudomonas aeruginosa is a versatile gram negative ... Major Characteristics Of Pseudomonas Aeruginosa. 1517 Words , 7 Pages. I. Description of P. aeruginosa Pseudomonas aeruginosa ... More about Pseudomonas Aeruginosa Essay. *. Pseudomonas aeruginosa Essay. 2797 Words , 12 Pages ...
... Fco Javier Casas Ciria syslan2 at net.disbumad.es Fri Jan 31 18:08:17 EST 1997 * ... What meaning has to find in a pharyngeal cultivation a very abundant growth of Pseudomonas aeruginosa? When it appears a pure ...
... Designation: PRD-10 TypeStrain=False Application: Assay of antimicrobial preservatives ... Pseudomonas aeruginosa (ATCC® 15442™) Strain Designations: PRD-10 [CIP 103467, NCIB 10421, PCI 812] / Type Strain: no / ... Testing Disinfectants against Pseudomonas aeruginosa, Use-Dilution Method. Gaithersburg, MD:AOAC International;AOAC "Official ... Nucleotide (GenBank) : AF094718 Pseudomonas aeruginosa strain ATCC 15442 16S ribosomal RNA gene, partial sequence. ...
2007) Ethylene chemotaxis in Pseudomonas aeruginosa and other Pseudomonas species. Microbes Environ 22:186-189. ... 2007) PilJ localizes to cell poles and is required for type IV pilus extension in Pseudomonas aeruginosa. Curr Microbiol 55:389 ... The microorganism Pseudomonas aeruginosa is ubiquitous and the leading cause of nosocomial infections (1). It exhibits two ... 2002) Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response. J Bacteriol 184:4374- ...
2000) Pseudomonas aeruginosa cell-to-cell signaling is required for virulence in a model of acute pulmonary infection. Infect ... 2006) Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc Natl Acad Sci USA 103:8487- ... 2003) Role of the quorum-sensing system in experimental pneumonia due to Pseudomonas aeruginosa in rats. Am J Respir Crit Care ... 2002) Detection of Pseudomonas aeruginosa cell-to-cell signals in lung tissue of cystic fibrosis patients. Microb Pathog 32:143 ...
Pseudomonas aeruginosais one of the most commonly considered gram-negative aerobic bacilli in the differential diagnosis of ... Pseudomonas aeruginosa bacteremia and endocarditis. *Pseudomonas aeruginosa infections of the eye, ear, urinary tract, ... PCR-based assay for the rapid and precise distinction of Pseudomonas aeruginosa from other Pseudomonas species recovered from ... Pseudomonas aeruginosa skin and soft tissue infections. Authors. Souha S Kanj, MD. Souha S Kanj, MD ...
Pseudomonas is a gram-negative rod that belongs to the family Pseudomonadaceae. More than half of all clinical isolates produce ... encoded search term (Pseudomonas aeruginosa Infections) and Pseudomonas aeruginosa Infections What to Read Next on Medscape. ... Pseudomonas aeruginosa Infections. Updated: Dec 20, 2018 * Author: Marcus Friedrich, MD, MBA, FACP; Chief Editor: Michael ... Infections with Pseudomonas aeruginosa in Charcot arthropathy of the foot. Foot Ankle Int. 2013 Feb. 34(2):234-7. [Medline]. ...
highlight,,italic,Pseudomonas aeruginosa ,/italic,,/highlight,biofilm on a surface is eradicated or at least reduced using a ... Pseudomonas aeruginosa. : The Microbe and Pathogen", in Pseudomonas aeruginosa Infections and Treatment. , A. L. Baltch and R. ... Pseudomonas aeruginosa is a strain of bacteria which can form biofilm on surfaces in contact with water. As is well recognized ... Pseudomonas aeruginosa. -highly resistant slime layers can be formed on a clean surface in less than one week. In addition, ...
... Designation: R. Hugh 813 TypeStrain=False Application: For use in testing and ... Pseudomonas aeruginosa (ATCC® CRM-9027™) Strain Designations: R. Hugh 813 / Type Strain: no / Biosafety Level: 2 ... Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa. London, UK:British Standards Institution;British Standard BS ... Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl. Environ. ...
Adaptation and diversification in virulence factors among urinary catheter-associated Pseudomonas aeruginosa isolates. The aim ... In patients with cUTI, Pseudomonas aeruginosa deserves special attention, since it can affect patients with serious underlying ... Risk factors and prognosis of complicated urinary tract infections caused by Pseudomonas aeruginosa in hospitalized patients: a ... biofilm formation and virulence among Pseudomonas aeruginosa isolated from the catheter-associated urinary tract infection ( ...
Oriol Sibila, Lídia Perea, Elisabet Cantó, Amelia Shoemark, Diane Cassidy, Alexandria Holly Smith, Guillermo Suarez-Cuartin, Ana Rodrigo-Troyano, Holly R Keir, Martina Oriano, Samantha Ong, Silvia Vidal, Francesco Blasi, Stefano Aliberti, James D Chalmers ...
Because bacteria cause Pseudomonas aeruginosa infections, doctors treat them with antibiotics, reports WebMD. Sometimes doctors ... Pseudomonas aeruginosa infections are most dangerous at hospitals, where patients already have weakened immune systems from ... Multi-drug resistant Pseudomonas aeruginosa infections are sometimes fatal to critical care patients. People using swimming ... The best way to avoid Pseudomonas aeruginosa infections is to practice good hygiene to prevent its spread, advises WebMD. In ...
S. Häussler and T. Becker, "The pseudomonas quinolone signal (PQS) balances life and death in Pseudomonas aeruginosa ... "Influence of the Pseudomonas quinolone signal on denitrification in Pseudomonas aeruginosa," Journal of Bacteriology, vol. 190 ... E. C. Pesci, J. P. Pearson, P. C. Seed, and B. H. Iglewski, "Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa ... M. S. Son, W. J. Matthews, Y. Kang, D. T. Nguyen, and T. T. Hoang, "In vivo evidence of Pseudomonas aeruginosa nutrient ...
Here we investigate the impact of indole and 7HI on Pseudomonas aeruginosa PAO1 virulence and quorum sensing (QS)-regula … ... Indole and 7-hydroxyindole diminish Pseudomonas aeruginosa virulence Microb Biotechnol. 2009 Jan;2(1):75-90. doi: 10.1111/j. ... Here we investigate the impact of indole and 7HI on Pseudomonas aeruginosa PAO1 virulence and quorum sensing (QS)-regulated ... Furthermore, 7HI reduced pulmonary colonization of P. aeruginosa in guinea pigs and increased clearance in lungs. Hence, indole ...
Pseudomonas aeruginosa, a gram-negative nonfermenting bacillus, is a much-feared pathogen. The organism is common in the ... Pseudomonas aeruginosa killing of Caenorhabditis elegans used to identify P. aeruginosa virulence factors. Proc Natl Acad Sci U ... Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc Natl Acad Sci U S A 2006; 103: ... Pseudomonas aeruginosa alginate is refractory to Th1 immune response and impedes host immune clearance in a mouse model of ...
Pseudomonas aeruginosa, is an important etiologic agent of a variety of infectious diseases affecting the eye and surrounding ... The number of clinical studies and studies with mouse, guinea pig, and rabbit models of Pseudomonas-induced ocula … ... Pathogenesis of Pseudomonas aeruginosa ocular diseases Rev Infect Dis. Nov-Dec 1983;5 Suppl 5:S931-5. doi: 10.1093/clinids/5. ... The gram-negative opportunistic bacterial pathogen, Pseudomonas aeruginosa, is an important etiologic agent of a variety of ...
The Pseudomonas aeruginosa Population among Cystic Fibrosis Patients in Quebec, Canada: a Disease Hot Spot without Known ... Pseudomonas aeruginosa is an opportunistic human pathogen that frequently causes health care-associated infections (HAIs). Due ... A Selective Culture Medium for Screening Ceftazidime-Avibactam Resistance in Enterobacterales and Pseudomonas aeruginosa The ... The prevalence of carbapenem-resistant Pseudomonas aeruginosa is increasing. Identification of carbapenemase-producing P. ...
Requirements for the operation of various types of bodies performing inspection for risk assessment for Pseudomonas aeruginosa ... and suggestions for the project to evolve to include a number of water quality parameters and not just pseudomonas aeruginosa, ...
Pseudomonas Aeruginosa - Jordan Short by Jordan Short , This newsletter was created with Smore, an online tool for creating ... Pseudomonas Aeruginosa is an environmental bacteria that is in the top 3 leading causes of opportunistic infections. It causes ...
Batch cultures of Pseudomonas aeruginosawere able to produce only low levels of cyanide during logarithmic growth with adequate ... Glycine metabolism byPseudomonas aeruginosa: Hydrogen cyanide biosynthesis. Journal of Bacteriology130:826-831.PubMedGoogle ... Batch cultures ofPseudomonas aeruginosa were able to produce only low levels of cyanide during logarithmic growth with adequate ... The effect of inorganic phosphate on cyanogenesis byPseudomonas aeruginosa. Archives of Microbiology114:51-54.PubMedGoogle ...
... Paola R. Beassoni, Lucas A. ... Paola R. Beassoni, Lucas A. Gallarato, Cristhian Boetsch, Mónica N. Garrido, and Angela T. Lisa, "Pseudomonas aeruginosa ...
Kerr KG, Snelling AM (2009) Pseudomonas aeruginosa: a formidable and ever-present adversary. J Hosp Infect 73:338-344CrossRef ... Tang HB, DiMango E, Bryan R, Gambello M, Iglewski BH, Goldberg JB et al (1996) Contribution of specific Pseudomonas aeruginosa ... van Heeckeren AM, Schluchter MD, Xue W, Davis PB (2006) Response to acute lung infection with mucoid Pseudomonas aeruginosa in ... Gellatly SL, Hancock RE (2013) Pseudomonas aeruginosa: new insights into pathogenesis and host defenses. Pathog Dis 67:159-173 ...
Find proteins for Q9I2A1 (Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS ... Crystal structure of Pseudomonas aeruginosa HmgR. Gu, T., Ji, Q., Liang, H.. To be published. ... Pseudomonas aeruginosa. Mutation(s): 0 Gene Names: kdgR_2, AOY09_03044, PAERUG_P32_London_17_VIM_2_10_11_04319. ...
  • Pseudomonas is a type of bacteria (germ) that is found commonly in the environment, like in soil and in water. (cdc.gov)
  • If bacteria are identified from the culture, perform antimicrobial susceptibility testing to guide treatment, and test carbapenem-resistant Enterobacteriaceae , Acinetobacter baumannii , and Pseudomonas aeruginosa for VIM and other plasmid-mediated carbapenemases. (cdc.gov)
  • The second component of Quorum sensing in P. aeruginosa bacteria is called the "rhl system", named due to its ability to control the gene expression which ultimately produces rhamnolipid. (news-medical.net)
  • These bacteria already produce the endotoxins required to initiate disease, however, the coordinated expression of these virulence genes by whole, densely-packed populations allow for P. aeruginosa populations to effectively overcome the host defences, and cause infection at a more efficient rate. (news-medical.net)
  • The ceftazidime component has activity against gram-negative bacteria, including P aeruginosa . (medscape.com)
  • Unlike many environmental bacteria, Pseudomonas aeruginosa has a remarkable capacity to cause disease in susceptible hosts. (bartleby.com)
  • This colorized version of PHIL 232 depicts a scanning electron micrograph (SEM) of a number of Pseudomonas aeruginosa bacteria. (asknature.org)
  • Because bacteria cause Pseudomonas aeruginosa infections, doctors treat them with antibiotics, reports WebMD. (reference.com)
  • Pseudomonas Aeruginosa is an environmental bacteria that is in the top 3 leading causes of opportunistic infections. (smore.com)
  • Pseudomonas aeruginosa (P. aeruginosa) is a Gram-negative bacteria that often occurs in soil and ground water. (news-medical.net)
  • The majority of bacteria require either flagella or type IV pili (TFP) to move, but P. aeruginosa is one of the few bacteria that requires both of these appendages. (news-medical.net)
  • Secondly, P.aeruginosa is the leading etiology for Gram-negative bacteria at most medical centers, carrying a 40-60% mortality rate. (kenyon.edu)
  • The genomes of P. aeruginosa strains are larger than those of most sequenced bacteria. (frontiersin.org)
  • In chinchillas, infection with Pseudomonas aeruginosais bacteria is the most common bacterial infection. (petmd.com)
  • This is mainly because Pseudomonas aeruginosa are mainly found in unclean environments, and when the immunity of the chinchillas is compromised or reduced, the bacteria gain an upper hand and cause disease. (petmd.com)
  • Pseudomonas aeruginosa is a disease-producing bacteria found in unclean drinking water and cages or contaminated fecal droppings. (petmd.com)
  • Confirmation is based on a positive identification of colonies of Pseudomonas aeruginosa bacteria. (petmd.com)
  • Considering taking medication to treat joint+infection+caused+by+pseudomonas+aeruginosa+bacteria? (webmd.com)
  • Below is a list of common medications used to treat or reduce the symptoms of joint+infection+caused+by+pseudomonas+aeruginosa+bacteria. (webmd.com)
  • P. aeruginosa - Xen05 bioluminescent pathogenic bacteria for in vivo and in vitro drug discovery. (perkinelmer.com)
  • Pseudomonas aeruginosa is an opportunistic gram-negative bacteria that lives in soil, water, and even in environments like hot tubs. (marketpublishers.com)
  • Antibodies against Pseudomonas aeruginosa (PA) lectin, PAIIL, which is a virulence factor mediating the bacteria binding to epithelium cells, were prepared in chickens and purified from egg yolks. (sigmaaldrich.com)
  • Healthcare-associated infections caused by bacteria such as Pseudomonas aeruginosa are a major public health problem worldwide. (scielo.br)
  • HAI are often caused by multidrug-resistant (MDR) bacteria, such as Pseudomonas aeruginosa , especially in immunocompromised patients. (scielo.br)
  • Pseudomonas aeruginosa bacteria, coloured scanning electron micrograph (SEM). (sciencephoto.com)
  • Pseudomonas aeruginosa bacteria inside biofilm, computer illustration. (sciencephoto.com)
  • The generic name ''Pseudomonas'' created for these organisms was defined in rather vague terms in 1894 as a genus of Gram-negative, rod-shaped and polar-flagella bacteria. (wikidoc.org)
  • About 60% of people with cystic fibrosis have a chronic respiratory infection caused by a bacteria called Pseudomonas aeruginosa. (verywell.com)
  • The bacteria Pseudomonas aeruginosa lives all around us. (verywell.com)
  • In contrast to Escherichia coli , a model organism for chemotaxis that has 5 chemoreceptors and a single chemosensory pathway, Pseudomonas aeruginosa PAO1 has a much more complex chemosensory network, which consists of 26 chemoreceptors feeding into four chemosensory pathways. (pnas.org)
  • The hol gene (PRF9) product (117 aas) of Pseudomonas aeruginosa PAO1 exhibits a hydrophobicity profile similar to holins of P2 and φCTX phages with two peaks of hydrophobicity that might correspond to either one or two transmembrane segments (TMSs). (wikipedia.org)
  • In this study we looked at the effect of three different sets of Fe 3 O 4 nanoparticles (FeNPs) on the development of Pseudomonas aeruginosa PAO1 biofilms. (scirp.org)
  • The usual strain is Pseudomonas aeruginosa PAO1 (ATCC 15692). (protocol-online.org)
  • In 2000, the genome sequence of P. aeruginosa PAO1 strain was published, providing data regarding its genetic complexity and ecological versatility. (scielo.br)
  • more recently the sequence of other strains have been determined including ''P. aeruginosa'' strains PAO1 (2000), ''P. putida'' KT2440 (2002), ''P. fluorescens'' Pf-5 (2005), ''P. syringae'' pathovar tomato DC3000 (2003), ''P. syringae'' pathovar syringae B728a (2005), ''P. syringae'' pathovar phaseolica 1448A (2005), ''P. fluorescens'' PfO-1 and ''P. entomophila'' L48. (wikidoc.org)
  • Sub-MICs of the 14-membered macrolides erythromycin (EM) and clarithromycin (CAM) decreased the growth of Pseudomonas aeruginosa PAO1 and increased its sensitivity to endogenous and exogenous nitrosative stress. (asm.org)
  • Pseudomonas aeruginosa is a human pathogen which can cause life-threatening illness, especially in people who have a depleted immune system. (news-medical.net)
  • Background information of PAL Pseudomonas aeruginosa is a gram-negative bacterium that can pose as a pathogen for plants and animals. (bartleby.com)
  • Studies using the opportunistic pathogen Pseudomonas aeruginosa suggest that QS "cheats"-individuals that don't respond to the QS signal, but are still able to use public goods produced by others-have a selective advantage in the presence of QS cooperators. (pnas.org)
  • P seudomonas aeruginosa is an opportunistic pathogen responsible for acute infections in immunocompromised hosts and for chronic diseases in cystic fibrosis (CF) patients. (pnas.org)
  • Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa , lacking the capacity to degrade nitric oxide during anaerobic respiration," EMBO Journal , vol. 26, no. 15, pp. 3662-3672, 2007. (hindawi.com)
  • Hence, indole-related compounds have potential as a novel antivirulence approach for the recalcitrant pathogen P. aeruginosa. (nih.gov)
  • Pseudomonas aeruginosa , a gram-negative nonfermenting bacillus, is a much-feared pathogen. (uptodate.com)
  • The gram-negative opportunistic bacterial pathogen, Pseudomonas aeruginosa, is an important etiologic agent of a variety of infectious diseases affecting the eye and surrounding tissues. (nih.gov)
  • Pseudomonas aeruginosa is an opportunistic human pathogen that frequently causes health care-associated infections (HAIs). (asm.org)
  • This chapter describes methods to study bacterial pathogenesis in murine acute and persistent/relapsing infection models, using the Gram-negative bacterial pathogen Pseudomonas aeruginosa as an example. (springer.com)
  • P.aeruginosa is an opportunistic human pathogen. (kenyon.edu)
  • The plasmids, TEM, OXA, and PSE, for instance, are encoded for betalactamase production, which is necessary for its resistance to antibiotics, thus allowing P. aeruginosa to be a formidable pathogen (8). (kenyon.edu)
  • Pseudomonas aeruginosa is an opportunistic pathogen of humans. (emsl.com)
  • Pseudomonas aeruginosa is an opportunistic pathogen that may cause severe invasive diseases in critically ill patients. (genome.jp)
  • A team of clinician researchers has discovered a highly virulent, multidrug resistant form of the pathogen, Pseudomonas aeruginosa , in patient samples in Ohio. (eurekalert.org)
  • Although an anti-Psl mAb activated complement and enhanced neutrophil recognition of P . aeruginosa , neutrophil-mediated clearance of the pathogen was suboptimal owing to a second subversion mechanism, namely the type 3 secretion (T3S) injectisome. (jci.org)
  • In cystic fibrosis, Pseudomonas aeruginosa is a much-feared pathogen. (medicalxpress.com)
  • Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen found ubiquitously in both natural and manmade environments. (harvard.edu)
  • Pseudomonas aeruginosa (Pa) is the most important pathogen infecting the CF lower airways, and its acquisition early in life is associated with a pro-inflammatory effect, lower lung function, poor nutritional outcomes, and decreased survival. (clinicaltrials.gov)
  • It will provide a deeper understanding of the pathogen to physicians and surgeons caring for patients infected, or at risk of becoming infected, with Pseudomonas Aeruginosa . (abebooks.com)
  • P. aeruginosa is a versatile pathogen that can cause several types of infections affecting the lower respiratory tract, skin, urinary tract and eyes, leading to bacteraemia, endocarditis and other complications. (scielo.br)
  • Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections. (asm.org)
  • Pseudomonas aeruginosa'' is increasingly recognized as an emerging [[opportunistic pathogen]] of clinical relevance. (wikidoc.org)
  • Pseudomonas aeruginosa is an opportunistic bacterial pathogen and is known to produce biofilms. (asm.org)
  • Adaptation and diversification in virulence factors among urinary catheter-associated Pseudomonas aeruginosa isolates. (urotoday.com)
  • The growing prevalence and diversity of carbapenemase producers among carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates warrants an expansion of detection capabilities. (asm.org)
  • Pseudomonas aeruginosa isolates recovered from clinical and envir. (ingentaconnect.com)
  • The study was aimed at determining the prevalent biotypes of Pseudomonas aeruginosa in the environment of Buea and the susceptibility of isolates to antibiotics. (ingentaconnect.com)
  • Interplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates. (genome.jp)
  • Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. (pearltrees.com)
  • Five MDR clinical lab isolates (LS 22, LS 23, LS 38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated. (pearltrees.com)
  • We analyzed the PAE using both agents alone and in combination and after once and several expositions of different isolates of Pseudomonas. (hu-berlin.de)
  • The clinical manifestations and management of other P. aeruginosa infections and the epidemiology and pathogenesis of infection with this organism are also discussed separately. (uptodate.com)
  • See 'Epidemiology, microbiology, and pathogenesis of Pseudomonas aeruginosa infection' . (uptodate.com)
  • The number of clinical studies and studies with mouse, guinea pig, and rabbit models of Pseudomonas-induced ocular disease has increased markedly in recent years, and this research has led to our improved understanding of factors relating to the pathogenesis and management of the diseases. (nih.gov)
  • Deziel E, Gopalan S, Tampakaki AP, Lepine F, Padfield KE, Saucier M et al (2005) The contribution of MvfR to Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regulation: multiple quorum sensing-regulated genes are modulated without affecting lasRI , rhlRI or the production of N -acyl-L-homoserine lactones. (springer.com)
  • Pathogenesis of septic shock in Pseudomonas aeruginosa. (sld.cu)
  • 9. Tang HB, Di Mango E, Brian R, Gambelio M, Iglewski B, Goldberg JB y Prince A. Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. (sld.cu)
  • H. Arai, "Regulation and function of versatile aerobic and anaerobic respiratory metabolism in Pseudomonas aeruginosa ," Frontiers in Microbiology , vol. 2, no. 103, 2011. (hindawi.com)
  • J. C. Comolli and T. J. Donohue, "Differences in two Pseudomonas aeruginosa cbb3 cytochrome oxidases," Molecular Microbiology , vol. 51, no. 4, pp. 1193-1203, 2004. (hindawi.com)
  • H. Arai, T. Kodama, and Y. Igarashi, "Cascade regulation of the two CRP/FNR-related transcriptional regulators (ANR and DNR) and the denitrification enzymes in Pseudomonas aeruginosa ," Molecular Microbiology , vol. 25, no. 6, pp. 1141-1148, 1997. (hindawi.com)
  • Several factors contribute to the success of P. aeruginosa including the resistance factors many strains have acquired, as well as its ability to use a broad spectrum of nutrients, meaning it can grow in hospital sinks, drains and even in disinfectants. (news-medical.net)
  • TFP-deficient mutant strains of P. aeruginosa have demonstrated an increased swarming phenotype, which has led some researchers to ask what exactly is the role that TFP plays in swarming? (news-medical.net)
  • The team found that as opposed to TFP-deficient strains, P. aeruginosa wild type restrict their collective motion by using TFP to interact with each other and slow down swarming. (news-medical.net)
  • P. aeruginosa has many strains, including Pseudomonas aeruginosa strain PA01, Pseudomonas aeruginosa PA7, Pseudomonas aeruginosa strain UCBPP-PA14, and Pseudomonas aeruginosa strain 2192 (5). (kenyon.edu)
  • P. aeruginosa strains are found in various environmental habitats as well as in animal and human hosts, where they can act as opportunistic pathogens. (frontiersin.org)
  • Though approximately 90% of the P. aeruginosa genome is shared between different strains, a team of scientist led by Dr. Craig Winstanley of the University of Liverpool set out to investigate the unique genomic features of the Liverpool strain. (cshlpress.com)
  • C ) Quantification of SD-IVM images for number of Kupffer cells associated with P . aeruginosa -GFP was assessed at 15 minutes after i.v. infection with WT, Δ pslA , or Δ pcrV strains. (jci.org)
  • In the present study total of 76 strains of P. aeruginosa were characterized (54 strains were isolated from soil samples collected from a crude oil contaminated site located north of Kuwait and 22 strains were isolated from medical samples). (witpress.com)
  • Whereas the phenotypic characterization such as the biochemical tests using VITEK 2 system (bioMérieux) and antibiotics susceptibility testing (AST) showed the high similarity between the medical and the environmental strains, the molecular analyses based on multilocus sequence typing (MLST) and restriction fragment length polymorphism (RFLP) demonstrated the heterogeneity of isolated P. aeruginosa indicating the presence of different clusters. (witpress.com)
  • Drug-resistant strains of Pseudomonas are a serious health problem, so this family of antibiotics may have important therapeutic applications. (sciencemag.org)
  • L27-11 possessed an interesting spectrum of antimicrobial activity, including minimal inhibitory concentrations (MICs) in the nanomolar range against many Pseudomonas aeruginosa ( PA ) strains and other Pseudomonas spp. (sciencemag.org)
  • In bacterial uptake and tolerance to heavy metals, Pseudomonas aeruginosa recorded potential role in bioremediation of different heavy metals with high removal percentage comparison with other bacterial strains. (intechopen.com)
  • In Brazil, P. aeruginosa was ranked as the fifth most common causative agent of HAI in patients hospitalised in adult and paediatric intensive care units, and nearly 35% of the reported strains are resistant to carbapenems, a class of antibiotics widely used to treat P. aeruginosa infections. (scielo.br)
  • Four sequence type 664 (ST664) (serotype O:5) strains of Pseudomonas aeruginosa that were highly resistant to antibiotics, including ceftolozane-tazobactam and ceftazidime-avibactam, but were susceptible to colistin were found to harbor the gene encoding the rare class C β-lactamase PAC-1 on a chromosomally located Tn 1721 -like transposon. (asm.org)
  • Also, the production of biofilms - a complex collection of surface-associated bacterial cells, is a common cause of persistent infections, including P. aeruginosa infections. (news-medical.net)
  • In a study performed in 2004, two different synthetic furanones were examined to find out their ability to cure lung infections in mice, by inhibiting Quorum sensing within the P. aeruginosa biofilms, which were causing the infection. (news-medical.net)
  • Quorum sensing in Pseudomonas aeruginosa biofilms. (scirp.org)
  • 2005. Iron salts perturb biofilm formation and disrupt existing biofilms of Pseudomonas aeru-ginosa. (scirp.org)
  • The ability of P. aeruginosa to form biofilms also protects it from antibiotics and host defence mechanisms. (news-medical.net)
  • Understanding how the bacterial cells interact and coordinate in space in order to colonize new surfaces can help researchers establish how P. aeruginosa behaves in order to form biofilms. (news-medical.net)
  • Both flagella and TFP are required in order for P. aeruginosa to form biofilms and attach to surfaces such as eukaryotic epithelial cells. (news-medical.net)
  • Complex differentiation within biofilms of Pseudomonas aeruginosa occurs, leading to the creation of voids inside microcolonies and to the dispersal of cells from within these voids. (asm.org)
  • Here we report that a repeatable pattern of cell death and lysis occurs in biofilms of P. aeruginosa during the normal course of development. (asm.org)
  • The differentiated microcolony phenotype has been found in most bacterial biofilms studied to date, including those of Escherichia coli ( 10 ), Pseudomonas aeruginosa ( 14 , 34 ), and Vibrio cholerae ( 65 ). (asm.org)
  • Resistance of artificial biofilms of Pseudomonas aeruginosa to imipenem and tobramycin. (biomedsearch.com)
  • Common places for biofilms of P. aeruginosa to develop are on contact lenses, where they can cause eye infections, on catheters, where they cause catheter-associated infections, and on wound surfaces, where they cause wound infections. (sciencephoto.com)
  • Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients," Journal of Clinical Investigation , vol. 109, no. 3, pp. 317-325, 2002. (hindawi.com)
  • P. aeruginosa is the most common cause of persistent and fatal lung infections in cystic fibrosis patients. (cshlpress.com)
  • The Liverpool Epidemic Strain, the most common strain of P. aeruginosa infecting cystic fibrosis patients in the United Kingdom, is characterized by its particular aggressiveness and virulence. (cshlpress.com)
  • Winstanley also explained that this work is especially important in light of the nature of P. aeruginosa lung infections that afflict cystic fibrosis patients. (cshlpress.com)
  • Purpose of review Recent articles of clinical interest on Pseudomonas aeruginosa respiratory tract infections including CAP, nosocomially-acquired pneumonia, particularly in the ventilated patient, and chronic infections in cystic fibrosis patients are reviewed. (ovid.com)
  • Pseudomonas aeruginosa infections are generally treated with antibiotics. (cdc.gov)
  • P. aeruginosa expressing VIM (VIM-CRPA) are often resistant to all first-line antibiotics and can cause infections that are difficult to treat. (cdc.gov)
  • P. aeruginosa expressing VIM (VIM-CRPA) are often resistant to all first-line antibiotics and difficult to treat, requiring complex antibacterial drug combinations and prolonged courses. (cdc.gov)
  • By using furanones to prevent quorum sensing and reduce biofilm formation, severe Pseudomonas aeruginosa infections could be more easily treated with antibiotics. (news-medical.net)
  • P. aeruginosa is usually treated with antibiotics, but the infection is becoming more difficult to treat because it has become resistant to many of the most commonly used antibiotics. (news-medical.net)
  • December 2, 2008 - The bacterium Pseudomonas aeruginosa is well known for its environmental versatility, ability to cause infection in humans, and its capacity to resist antibiotics. (cshlpress.com)
  • CHICAGO-Fifteen to 20 years ago, treatment of patients infected with Pseudomonas aeruginosa infection after stem cell transplantation was limited to certain beta-lactam and aminoglycoside antibiotics that were active against the organism. (cancernetwork.com)
  • Because newer antibiotics, such as the fluoroquinolones and the cephalosporins cefotaxime (Claforan) and cefepime (Maxipime), have greater activity against P aeruginosa and less toxicity than previously prescribed antibiotics, there has been every reason to believe that treatment has improved and mortality has declined. (cancernetwork.com)
  • We have no randomized data to suggest that using treatment longer than the standard course of antibiotics would work, but one option might be to extend treatment with an oral agent that is not too toxic and has activity against P aeruginosa, such as one of the fluoroquinolones, beyond the usual 2 to 3 weeks of initial intravenous therapy,' Dr. Hakki commented. (cancernetwork.com)
  • We synthesized a family of peptidomimetic antibiotics based on the antimicrobial peptide protegrin I. Several rounds of optimization gave a lead compound that was active in the nanomolar range against Gram-negative Pseudomonas spp. (sciencemag.org)
  • Although some studies have shown that a minority of individuals with CF spontaneously clear early Pseudomonas aeruginosa (Pa) infection, data from multiple studies suggest that antibiotics are superior to no treatment in clearing Pseudomonas aeruginosa (Pa) from respiratory cultures. (clinicaltrials.gov)
  • Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide and notorious for its broad-spectrum resistance to antibiotics. (asm.org)
  • Years ago, chronic Pseudomonas aeruginosa infection in people with CF was treated rather unsuccessfully with oral antibiotics or inhaled injectable formulations of antibiotics . (verywell.com)
  • Inhaled antibiotics, particularly tobramycin, are increasingly used for the prevention and treatment of P. aeruginosa infection in CF patients. (ovid.com)
  • The clinical manifestations, diagnosis, and treatment of P. aeruginosa skin, soft tissue, and bone infections will be reviewed here. (uptodate.com)
  • One hundred and fifty clinical specimens (urine, wound and sputum) collected from patients attending various health institutions in Buea, and 50 environmental swabs from furniture, appliances and surroundings of these institutions were screened for P. aeruginosa using standard microbiological and biochemical methods. (ingentaconnect.com)
  • Based on the clinical picture we suspected preponderant P. aeruginosa bacteremia, outgrown by concomitant low-grade E. coli bacteremia in the blood culture vials. (frontiersin.org)
  • The clinical suspicion was EG due to Pseudomonas aeruginosa bacteremia, therefore amikacin was added, but the patient's condition rapidly deteriorated, and he died on day 12. (frontiersin.org)
  • The study will assess the clinical and microbiologic efficacy and safety of azithromycin given three times weekly in combination with standardized tobramycin solution for inhalation (TIS) therapy among children with early Pseudomonas aeruginosa (Pa). (clinicaltrials.gov)
  • In 2003, the first clinical isolate of an MDR P. aeruginosa strain carrying the carbapenemase gene, bla SPM-1 , was identified in Brazil. (scielo.br)
  • In clinical care, Pseudomonas aeruginosa is one of the leading causes of bacterial infections among patients with cystic fibrosis or compromised immune systems. (aiche.org)
  • Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes," Proceedings of the National Academy of Sciences of the United States of America , vol. 91, no. 1, pp. 197-201, 1994. (hindawi.com)
  • P. aeruginosa can carry genes encoding carbapenemases. (mn.us)
  • That indicated that a recombination event had occurred between Salmonella and P. aeruginosa , contributing even more resistance genes to the latter. (eurekalert.org)
  • Also, P. aeruginosa is responsible for nosocomial infections and usually harbour several antibiotic resistance genes. (witpress.com)
  • Biofilm formation and the presence of intrinsic resistance-associated genes are examples of the mechanisms that P. aeruginosa employs to resist chemotherapy. (scielo.br)
  • Full results from the phase III trials and future studies are likely to expand the use of ceftazidime/avibactam to include hospitalized adults with nosocomial and ventilator-associated pneumonia, hospitalized pediatric patients aged 3 months to 18 years with complicated intra-abdominal infections, and patients with cystic fibrosis who have resistant respiratory P aeruginosa infections. (medscape.com)
  • The purpose of this trial is to compare the effects of treatment with tobramycin solution for inhalation (TIS) with and without azithromycin in people with cystic fibrosis (CF) age 6 months to 18 years who have early isolation of Pseudomonas aeruginosa (Pa) from a respiratory culture. (clinicaltrials.gov)
  • TIS therapy is defined as an initial eradication treatment with 1-2 courses of 28 days TIS and subsequent 28 day treatments only at times a quarterly respiratory culture is positive for Pseudomonas aeruginosa (Pa). (clinicaltrials.gov)
  • At each visit, participants will undergo a physical examination, a spirometry test (as appropriate), a respiratory specimen for Pseudomonas aeruginosa (Pa) culture will be collected and study drug will be dispensed (except at Week 78). (clinicaltrials.gov)
  • More importantly, the overexpressed hPLUNC in Scgb1a1-hPLUNC transgenic mouse airway significantly enhanced mouse survival against P. aeruginosa -induced respiratory infection. (jimmunol.org)
  • Once Pseudomonas aeruginosa invades the respiratory tract, it is hard to get rid of. (verywell.com)
  • In the late 1990s an inhaled form of the antibiotic tobramycin, or TOBI, was developed specifically for the treatment of chronic respiratory Pseudomonas aeruginosa infections. (verywell.com)
  • In CF, the knowledge of the pharmacokinetics and bioavailability of inhaled tobramycin and its long term beneficial effect in lung function are important developments in this area Summary P. aeruginosa continues to be a serious problem worlwide as a cause of respiratory tract infections in selected populations. (ovid.com)
  • Unfortunately, in people exposed to healthcare settings like hospitals or nursing homes, Pseudomonas aeruginosa infections are becoming more difficult to treat because of increasing antibiotic resistance. (cdc.gov)
  • Learn more about how CDC's Antibiotic Resistance Laboratory Network detects highly resistant Pseudomonas aeruginosa infections. (cdc.gov)
  • Carbenicillin resistance in Pseudomonas aeruginosa. (bmj.com)
  • Black W A , Girdwood R W . Carbenicillin resistance in Pseudomonas aeruginosa. (bmj.com)
  • A cystic fibrosis epidemic strain of Pseudomonas aeruginosa displays enhanced virulence and antimicrobial resistance. (nih.gov)
  • Lipovy B, Rihova H, Hanslianova M, Gregorova N, Suchanek I, Brychta P (2010) Prevalence and resistance of Pseudomonas aeruginosa in severely burned patients: a 10-year retrospective study. (springer.com)
  • Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium. (genome.jp)
  • Bonomo RA, Szabo D. Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa. (springer.com)
  • Abstract In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. (pearltrees.com)
  • Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: Our worst nightmare? (sld.cu)
  • In Pseudomonas aeruginosa , the MexXY pump provides intrinsic resistance to many antimicrobials, including aminoglycosides. (asm.org)
  • In this article, we show that the average cost of rifampicin resistance in the pathogenic bacterium Pseudomonas aeruginosa is reduced by the addition of ribosome inhibitors (chloramphenicol or streptomycin) that indirectly constrain transcription rate and therefore reduce demand for RNA polymerase activity. (genetics.org)
  • To estimate the contribution of demand for RNA polymerase to the cost of resistance in rifampicin-free environments, we measured the fitness of 53 rifampicin-resistant mutants of Pseudomonas aeruginosa across a range of environments where we experimentally manipulated demand for RNA polymerase by adding sublethal doses of ribosomal inhibitors. (genetics.org)
  • In 2017, multidrug-resistant Pseudomonas aeruginosa caused an estimated 32,600 infections among hospitalized patients and 2,700 estimated deaths in the United States [ Source: 2019 AR Threats Report ]. (cdc.gov)
  • For some multidrug-resistant types of Pseudomonas aeruginosa , treatment options might be limited. (cdc.gov)
  • CDC tracks Pseudomonas aeruginosa and the infections this germ can cause, including antibiotic-resistant infections. (cdc.gov)
  • Since July 2020, CDC has received eight reports of highly antibiotic-resistant Pseudomonas aeruginosa infections in patients who underwent surgery or other invasive procedures in Mexico. (cdc.gov)
  • Highly antibiotic resistant Pseudomonas aeruginosa continue to be reported among travelers with infections who underwent surgery or invasive procedures at several healthcare facilities in Mexico. (cdc.gov)
  • Multi-drug resistant Pseudomonas aeruginosa infections are sometimes fatal to critical care patients. (reference.com)
  • The prevalence of carbapenem-resistant Pseudomonas aeruginosa is increasing. (asm.org)
  • An estimated 51,000 P. aeruginosa infections occur in the United States every year and more than 6,000 of those are multi-drug resistant. (news-medical.net)
  • These mechanisms mediate the multidrug-resistant (MDR) phenotype in P. aeruginosa. (genome.jp)
  • Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes. (genome.jp)
  • Complete genome sequence of highly multidrug-resistant Pseudomonas aeruginosa NCGM2.S1, a representative strain of a cluster endemic to Japan. (genome.jp)
  • New options of antibiotic combination therapy for multidrug-resistant Pseudomonas aeruginosa. (genome.jp)
  • The P. aeruginosa contained a gene for a drug resistant enzyme called a metallo beta-lactamase. (eurekalert.org)
  • Albany, NY, May 24, 2017 --( PR.com )-- Market Research Hub's latest Pharmaceutical and Healthcare disease pipeline guide Resistant Pseudomonas aeruginosa Infections Pipeline Review, H1 2017, provides an overview of the Resistant Pseudomonas aeruginosa Infections (Infectious Disease) pipeline landscape. (pr.com)
  • Global Markets Direct's Pharmaceutical and Healthcare latest pipeline guide Resistant Pseudomonas aeruginosa Infections Pipeline Review, H1 2017, provides comprehensive information on the therapeutics under development for Resistant Pseudomonas aeruginosa Infections (Infectious Disease), complete with analysis by stage of development, drug target, mechanism of action (MoA), route of administration (RoA) and molecule type. (pr.com)
  • The Resistant Pseudomonas aeruginosa Infections (Infectious Disease) pipeline guide also reviews of key players involved in therapeutic development for Resistant Pseudomonas aeruginosa Infections and features dormant and discontinued projects. (pr.com)
  • Resistant Pseudomonas aeruginosa Infections (Infectious Disease) pipeline guide helps in identifying and tracking emerging players in the market and their portfolios, enhances decision making capabilities and helps to create effective counter strategies to gain competitive advantage. (pr.com)
  • The pipeline guide provides a snapshot of the global therapeutic landscape of Resistant Pseudomonas aeruginosa Infections (Infectious Disease). (pr.com)
  • The pipeline guide reviews pipeline therapeutics for Resistant Pseudomonas aeruginosa Infections (Infectious Disease) by companies and universities/research institutes based on information derived from company and industry-specific sources. (pr.com)
  • The pipeline guide reviews key companies involved in Resistant Pseudomonas aeruginosa Infections (Infectious Disease) therapeutics and enlists all their major and minor projects. (pr.com)
  • The pipeline guide evaluates Resistant Pseudomonas aeruginosa Infections (Infectious Disease) therapeutics based on mechanism of action (MoA), drug target, route of administration (RoA) and molecule type. (pr.com)
  • In the cystic fibrosis lung, P. aeruginosa forms prominent microcolony structures ( 55 ) and is associated with antibiotic-resistant, often fatal infections ( 9 , 25 , 55 ). (asm.org)
  • The epidemiological profile of infections with multidrug-resistant Pseudomonas aeruginosa and Acinetobacter species. (springer.com)
  • Resistant Pseudomonas aeruginosa Infections (Infectious Disease) pipeline guide helps in identifying and tracking emerging players in the market and their portfolios, enhances decision making capabilities and helps to create effective counter strategies to gain competitive advantage Additionally, various dynamic tracking processes ensure that the most recent developments are captured on a real time basis. (medgadget.com)
  • Find and recognize significant and varied types of therapeutics under development for Resistant Pseudomonas aeruginosa Infections (Infectious Disease). (medgadget.com)
  • Formulate corrective measures for pipeline projects by understanding Resistant Pseudomonas aeruginosa Infections (Infectious Disease) pipeline depth and focus of Indication therapeutics. (medgadget.com)
  • Starting July 1, 2016 Carbapenemase Resistant Pseudomonas aeruginosa (CRPA) is reportable for residents of Hennepin and Ramsey Counties under Minnesota Rules 4605.7000 to 4605.7900. (mn.us)
  • Different resistant stains of Pseudomonas were explored versus amikacin and ceftazidime. (hu-berlin.de)
  • The goal of this study was to reconstruct the multidrug-resistant (MDR) P. aeruginosa GRN and to analyse its topological properties. (scielo.br)
  • The prevalence of multidrug-resistant Pseudomonas aeruginosa has led to the reexamination of older "forgotten" drugs, such as temocillin, for their ability to combat resistant microbes. (asm.org)
  • Given that aminoglycosides, such as amikacin, may be used for multidrug-resistant Pseudomonas aeruginosa infections, optimization of therapy is paramount for improved treatment outcomes. (asm.org)
  • Fluoroquinolones have a major role in the emergence of multiply resistant P. aeruginosa in patients with VAP. (ovid.com)
  • Patients with cystic fibrosis (CF) do not reveal a primary immune defect and respond with high numbers of functional polymorphonuclear leukocytes (PMN) and specific antibodies to lung infection with Pseudomonas aeruginosa . (ingentaconnect.com)
  • The present Competitive Intelligence Report about Pseudomonas aeruginosa Vaccines & Therapeutics provides a competitor evaluation in the field of prophylactic vaccines and therapeutic antibodies, peptides and small molecules as well as DNA-, RNA- and microorganism-based therapies against infection with Pseudomonas aeruginosa as of May 2012. (marketpublishers.com)
  • Bronchopulmonary infection with Pseudomonas aeruginosa in patients infected with human immunodeficiency virus. (bmj.com)
  • Maura D, Hazan R, Kitao T, Ballok AE, Rahme LG (2016) Evidence for direct control of virulence and defense dene circuits by the Pseudomonas aeruginosa quorum sensing regulator, MvfR. (springer.com)
  • In all oligotropic aquatic ecosystems, which contain high-dissolved oxygen content but low plant nutrients throughout, P.aeruginosa is the predominant inhabitant and this clearly makes it the most abundant organism on earth (2). (kenyon.edu)
  • Quorum sensing in P. aeruginosa has two different parts - the first being related to the expression of the gene encoding the protein LasB elastase. (news-medical.net)
  • In terms of human infection, the cell-to-cell interaction involved in Quorum sensing may have a role in enabling Pseudomonas aeruginosa to by-pass the host's immune system. (news-medical.net)
  • The study proved that furanones are able to successfully interact with the molecule N-acyl homoserine lactone, which in turn supresses P. aeruginosa biofilm Quorum sensing. (news-medical.net)
  • Retrieved on March 01, 2021 from https://www.news-medical.net/life-sciences/Quorum-Sensing-and-Pseudomonas-aeruginosa.aspx. (news-medical.net)
  • Venturi V (2006) Regulation of quorum sensing in Pseudomonas . (springer.com)
  • Gilbert KB, Kim TH, Gupta R, Greenberg EP, Schuster M (2009) Global position analysis of the Pseudomonas aeruginosa quorum-sensing transcription factor LasR. (springer.com)
  • 2008. Influence of quorum sensing and iron on twitching motility and biofilm formation in Pseudomonas aeruginosa. (scirp.org)
  • A recent study demonstrated that water channels around P. aeruginosa microcolonies are actively maintained by the quorum-sensing-controlled production of rhamnolipid surfactants ( 12 ). (asm.org)
  • To address this need, we report the use of an inexpensive, electrochemical sensor to detect pyocyanin, a unique, quorum sensing molecule secreted by Pseudomonas aeruginosa , in human wound exudate obtained from patients with chronic wounds. (aiche.org)
  • How do doctors treat Pseudomonas aeruginosa? (reference.com)
  • Tobramycin is an aminoglycoside antibiotic used to treat Pseudomonas aeruginosa infection. (verywell.com)
  • Understanding the transition period from early to chronic Pseudomonas aeruginosa (Pa) infection is thus of critical importance in identifying strategies to prevent this progression. (clinicaltrials.gov)
  • The bacterium Pseudomonas aeruginosa can thrive in environments as different as the moist, warm tissue in human lungs, and the dry, nutrient-deprived surface of an office wall. (pharmamicroresources.com)
  • Researchers at Brigham and Women's Hospital (BWH) have discovered a new vaccine candidate for the bacterium Pseudomonas aeruginosa taking advantage of a new mechanism of immunity. (infectioncontroltoday.com)
  • The study, presented in a poster session at the Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC abstract 780), was a retrospective review of 46 cases of invasive P aeruginosa infection in individuals who underwent hematopoietic stem cell transplantation between 1990 and 2000. (cancernetwork.com)
  • Here, we report the evolutionary dynamics and virulence of P. aeruginosa populations during lung colonization of mechanically ventilated patients in the absence of antimicrobial treatments. (pnas.org)
  • The mucoid character of P. aeruginosa , an altered epithelial cell surface, and high concentrations of PMN‐derived lysosomal enzymes contribute to impaired bacterial lung clearance and result in chronic infection. (ingentaconnect.com)
  • P . aeruginosa adhered avidly to lung vasculature, where patrolling neutrophils and other immune cells were virtually blind to the pathogen's presence. (jci.org)
  • Virulence factors from Pseudomonas aeruginosa increase lung epithelial permeability. (sld.cu)
  • Pseudomonas also causes lung infections in people with cystic fibrosis, a genetic disorder that renders the lungs susceptible to bacterial infection. (infectioncontroltoday.com)
  • Effect of anaerobiosis and nitrate on gene expression in Pseudomonas aeruginosa. (nih.gov)
  • In summary, P. aeruginosa is endowed with a highly conserved core genome of low sequence diversity and a highly variable accessory genome that communicates with other pseudomonads and genera via horizontal gene transfer. (frontiersin.org)
  • Here I describe TrrC, a novel sRNA in P. aeruginosa that positively regulates cupA fimbrial gene expression. (harvard.edu)
  • Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate. (pseudomonas.com)
  • In the current project, "Control of Na+ and H+ transport in bacterial adaptation," researchers will seek to understand how transport proteins that move hydrogen and sodium cations through the cell membrane allow Pseudomonas to adjust its metabolism to different environmental conditions. (pharmamicroresources.com)
  • Metabolism on aeroobne ja mitte kääritav , kuid siiski on Pseudomonas aeruginosa võimeline kasvama ka anaeroobsetes tingimustes , kui keskkonnas leidub NO 3 , mida kasutada elektronide aktseptorina. (wikipedia.org)
  • Effect of salt concentration on the apparent in-vitro susceptibility of Pseudomonas and other gram-negative bacilli to gentamicin. (atcc.org)
  • Pseudomonas Genome Database: facilitating user-friendly, comprehensive comparisons of microbial genomes. (nih.gov)
  • If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. (pseudomonas.com)
  • Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database. (pseudomonas.com)
  • Ecthyma Gangrenosum: Escherichia coli or Pseudomonas aeruginosa? (frontiersin.org)
  • In a patient with EG, initial blood cultures showed Escherichia coli , and almost occulted P. aeruginosa bacteremia. (frontiersin.org)
  • 2009. Iron-binding compounds impair Pseudomonas aeruginosa biofilm formation, especially under anaerobic conditions. (scirp.org)
  • Due to its capability to synthesize arginine, P. aeruginosa can also proliferate in anaerobic conditions. (kenyon.edu)
  • Pseudomonas aeruginosa on gramnegatiivne, aeroobne , kepikujuline Pseudomonadaceae seltsi ja gamma proteobakterite klassi kuuluv bakter. (wikipedia.org)
  • 2007. Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa. (scirp.org)
  • During the onset of biofilm killing and during biofilm development thereafter, a bacteriophage capable of superinfecting and lysing the P. aeruginosa parent strain was detected in the fluid effluent from the biofilm. (asm.org)
  • Staphylococcus, Bacillus and Pseudomonas spp. (scirp.org)
  • Bacillus pyocyaneus , Bakterium aeruginosa , Pseudomonas polycolor , and Pseudomonas pyocyaneus (3). (kenyon.edu)
  • P. aeruginosa is a Gram-negative bacillus that is typically 1-3 µm in length. (springer.com)
  • Recent findings The growing importance of P. aeruginosa as an etiologic agent of CAP, the occurrence of CAP in previously healthy adults and its high prevalence as an etiologic agent of late VAP are stressed in recent studies. (ovid.com)
  • RICHARDSON M. Pseudomonas aeruginosa septicemia. (jaoa.org)
  • Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. (pseudomonas.com)
  • In the current studies, the investigators designed a screen for Th17-stimulating protein antigens expressed by a molecular library of DNA encoding Pseudomonas proteins. (infectioncontroltoday.com)
  • The microorganism Pseudomonas aeruginosa is ubiquitous and the leading cause of nosocomial infections ( 1 ). (pnas.org)
  • 1999. The response of Pseudomonas aeruginosa to iron: Genetics, biochemistry and virulence. (scirp.org)
  • P. aeruginosa is a gram-negative bacterium whose virulence depends on a large number of extra- and intracellular factors. (news-medical.net)
  • Pseudomonas aeruginosa Pseudomonas aeruginosa is a versatile gram negative bacterium that grows in soil, marshes, and coastal marine habitats, as well as on plant and animal tissues. (bartleby.com)
  • The screen discovered that the Pseudomonas protein PopB is a very effective stimulator of Th17 immunity, and immunization with purified PopB protected mice from lethal pneumonia in an antibody-independent fashion. (infectioncontroltoday.com)
  • By messing with the protein synthesis of Pseudomonas aeruginosa, TOBI consequently disrupts the bacterial cell membrane and envelope thus causing cell death. (verywell.com)
  • Production of hybrid protein OprF-OprL of Pseudomonas aeruginosa]. (semanticscholar.org)
  • Safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein F-I vaccine in human volunteers. (semanticscholar.org)
  • The general principles of antimicrobial treatment of infections caused by P. aeruginosa , including antibiotic options and decisions on combination therapy, are discussed in detail elsewhere. (uptodate.com)
  • Risk factors and prognosis of complicated urinary tract infections caused by Pseudomonas aeruginosa in hospitalized patients: a retrospective multicenter cohort study. (urotoday.com)
  • 2008. Opportunistic infections caused by Pseudomonas aeruginosa. (scirp.org)
  • In this article a review of published works on research developed in different countries with the purpose to obtain vaccine candidates for the prevention or treatment of the infections caused by Pseudomonas aeruginosa beginning since the decade of the fifties of the 20th century up to the year 2003. (sld.cu)
  • P. aeruginosa was first described as a distinct bacterial species at the end of the nineteenth century, after the development of sterile culture media by Pasteur. (kenyon.edu)
  • The genetic repertoire of Pseudomonas aeruginosa reflects the lifestyle of this ubiquitous bacterial species. (frontiersin.org)
  • Ramphal R. Infections Due to Pseudomonas Species and Related Organisms. (verywell.com)
  • Lister PD, Gardner VM and Sanders CC. Clavulanate induces expression of the Pseudomonas aeruginosa AmpC cephalospori-nase at physiologically relevant concentrations and antagonizes the antibacterial activity of ticarcillin. (springer.com)
  • In a study published online today in Genome Research ( www.genome.org ), researchers have used genomic techniques to study a particularly virulent strain of P. aeruginosa, uncovering genetic clues to its success that will aid in the design of novel therapeutic strategies. (cshlpress.com)
  • Pseudomonas aeruginosa has become an important cause of gram-negative infection, especially in patients with compromised host defense mechanisms. (medscape.com)
  • P . aeruginosa has evolved numerous evasion and subversion mechanisms that work in concert to overcome immune recognition and effector functions in hospitalized and immunosuppressed individuals. (jci.org)
  • These data highlight the need to block multiple evasion and subversion mechanisms in tandem to kill P . aeruginosa . (jci.org)