Orf virus: The type species of PARAPOXVIRUS which causes a skin infection in natural hosts, usually young sheep. Humans may contract local skin lesions by contact. The virus apparently persists in soil.Ecthyma, Contagious: An infectious dermatitis of sheep and goats, affecting primarily the muzzle and lips. It is caused by a poxvirus and may be transmitted to man.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Herpesvirus 3, Human: The type species of VARICELLOVIRUS causing CHICKENPOX (varicella) and HERPES ZOSTER (shingles) in humans.Viral Proteins: Proteins found in any species of virus.Parapoxvirus: A genus of the family POXVIRIDAE, subfamily CHORDOPOXVIRINAE, which infect ungulates and may infect humans. ORF VIRUS is the type species.Pseudocowpox virus: A species of PARAPOXVIRUS causing a pox-like disease on udders of cows that may spread to milkers.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Poxviridae Infections: Virus diseases caused by the POXVIRIDAE.Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.RNA Viruses: Viruses whose genetic material is RNA.Goat Diseases: Diseases of the domestic or wild goat of the genus Capra.Vaccinia virus: The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Political Systems: The units based on political theory and chosen by countries under which their governmental power is organized and administered to their citizens.Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Genes, Reporter: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.Luminescent Proteins: Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Databases, Chemical: Databases devoted to knowledge about specific chemicals.Fomites: Inanimate objects that carry pathogenic microorganisms and thus can serve as the source of infection. Microorganisms typically survive on fomites for minutes or hours. Common fomites include CLOTHING, tissue paper, hairbrushes, and COOKING AND EATING UTENSILS.Epidemiology: Field of medicine concerned with the determination of causes, incidence, and characteristic behavior of disease outbreaks affecting human populations. It includes the interrelationships of host, agent, and environment as related to the distribution and control of disease.Cooking and Eating UtensilsArchivesVirology: The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.Potyvirus: A large genus of plant viruses of the family POTYVIRIDAE which infect mainly plants of the Solanaceae. Transmission is primarily by aphids in a non-persistent manner. The type species is potato virus Y.Vicia faba: A plant species of the genus VICIA, family FABACEAE. The edible beans are well known but they cause FAVISM in some individuals with GLUCOSEPHOSPHATE DEHYDROGENASE DEFICIENCY. This plant contains vicine, convicine, Vicia lectins, unknown seed protein, AAP2 transport protein, and Vicia faba DNA-binding protein 1.RNA Replicase: An enzyme that catalyses RNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time, and can initiate a chain de novo. (Enzyme Nomenclature, 1992, p293)Iridaceae: A monocot plant family of the Liliopsida class. It is classified by some in the Liliales order and some in the Asparagales order.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Arteritis Virus, Equine: The type species of the genus ARTERIVIRUS and the etiologic agent of an important equine respiratory disease causing abortion, pneumonia, or other infections.Arterivirus Infections: Infections caused by viruses of the genus ARTERIVIRUS.Horse Diseases: Diseases of domestic and wild horses of the species Equus caballus.Arterivirus: A genus of the family ARTERIVIRIDAE, in the order NIDOVIRALES. The type species is ARTERITIS VIRUS, EQUINE.Hepatitis B e Antigens: A closely related group of antigens found in the plasma only during the infective phase of hepatitis B or in virulent chronic hepatitis B, probably indicating active virus replication; there are three subtypes which may exist in a complex with immunoglobulins G.Hepatitis B virus: The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum.Hepatitis B Vaccines: Vaccines or candidate vaccines containing inactivated hepatitis B or some of its component antigens and designed to prevent hepatitis B. Some vaccines may be recombinantly produced.Hepatitis B Surface Antigens: Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.Hepatitis B Antibodies: Antibodies to the HEPATITIS B ANTIGENS, including antibodies to the surface (Australia) and core of the Dane particle and those to the "e" antigens.Hepatitis B: INFLAMMATION of the LIVER in humans caused by a member of the ORTHOHEPADNAVIRUS genus, HEPATITIS B VIRUS. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact.Vaccines, Synthetic: Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.Peptide Library: A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.Host-Pathogen Interactions: The interactions between a host and a pathogen, usually resulting in disease.Cell Surface Display Techniques: Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.Bacteriophages: Viruses whose hosts are bacterial cells.Bacteriophage M13: Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.Oligopeptides: Peptides composed of between two and twelve amino acids.
A putative second ORF in the genome of an alphasatellite virus has been described. The significance of this finding (if any) is ... There are no distinctive virons because the viral genomes are encapsidated within the coat protein of the helper virus. ... These viruses were earlier known as DNA 1 components. These viruses are generally found in the Old World. A number have been ... four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome ...
Scans for natural selection showed that most codons of the SLE virus ORF were evolving neutrally or under negative selection. ... and recombination dynamics by sequencing the envelope protein gene and parts of other genes. A recent evolutionary study based ... Saint Louis encephalitis virus is related to Japanese encephalitis virus and is a member of the Flaviviridae subgroup. This ... Only infected mosquitoes can transmit Saint Louis encephalitis virus. Once a human has been infected with the virus it is not ...
The capsid protein of satellite panicum mosaic virus contributes to systemic invasion and interacts with its helper virus. ... PMV was placed in the genus Panicovirus because of its limited monocot host range and lack of an additional 5'-ORF. PMV is ... There exists a satellite virus to panicum mosaic virus. Although little is known about the satellite panicum mosaic virus, the ... Viruses and virus diseases of Poaceae (Gramineae). Inra. p. 798. ISBN 2-7380-1088-1. ICTVdB - The Universal Virus Database, ...
ORF I - Movement Protein ORF II - Insect Transmission Factor ORF III - Structural Protein, DNA-Binding Capabilities ORF IV - ... The mechanism of expression of these proteins is unique, in that the ORF VI protein (encoded by the 19S RNA) controls ... Capsid Protein ORF V - Protease, Reverse Transcriptase and RNaseH ORF VI - Translational Activator, Inclusion Body Formation/ ... 2012). "Cauliflower mosaic virus protein P6 inhibits signaling responses to salicylic acid and regulates innate immunity". PLoS ...
... two proteins are expressed from overlapping genes arranged so that the open reading frame of p19 is completely within the ORF ... The p22 protein is a movement protein that is required for the virus to spread from cell to cell. P22 is an RNA-binding protein ... Both proteins are associated with cell membranes. p41 (capsid protein). The viral capsid protein CP, or p41, is a ... a capsid protein (called CP or p41), and two additional proteins, the RNA silencing suppressor p19 and movement protein p22. ...
C1 protein is also known as the viral replication protein, which makes it essential for virus replication. C2, C3, and C4 ... The ssDNA genome encodes for six open reading frames (ORF): two in the virion sense orientation, V1 and V2, and four in the ... The function of the V1 protein, identified as the coat protein, is to encapsulate the ssDNA and form the virus particle to ... V1 protein is the coat protein and its function is to protect the viral DNA by encapsulating it. V2 protein is the pre-coat ...
ORF 2 contains the sequences for four structural proteins VP1, VP2, VP3, and minor protein VP4 which will be the main ... Triatoma virus (TrV) is a virus belonging to the insect virus family Dicistroviridae. Within this family, there are currently 3 ... TrV is a positive-sense, single-stranded RNA virus. It belongs to virus Group IV. Virus groups are based on the Baltimore ... The protein capsid of the virus is 30 nm in diameter. The capsid has icosahedral symmetry and a pseudo-triangulation number of ...
The ORF encodes a protein of 1549 amino acids with 8 potential sites for N-linked glycosylation. It contains a unique potential ... Kupe virus is a virus in the genus Orthonairovirus, order Bunyavirales. Ganjam virus lies within the Nairobi sheep disease ... The S segment has 1,694 nt and encodes an ORF of 483 amino acids. The 5′ and 3′ noncoding regions are 49 nt and 193 nt in ... The virus was isolated from pooled ticks (Amblyomma gemma and Rhipicephalus pulchellus) collected from cattle hides in Nairobi ...
The translation of the large ORF transcript produces a 114-kDa polyprotein. The mature VP4 protein, viral protease, assists ... and additional VP4 proteins. In addition VP3 proteins can associate with pre-VP2 as a structural protein and with VP1 to ... DXV is a Baltimore Class III naked (non-enveloped) virus. The capsid of this protein contains an icosahedral geometry (T=13) ... The replication of DXV follows the characterized dsRNA virus replication cycle. The large ORF of segment A consists of 3069 ...
Like all plant viruses, FMV encodes a movement protein (MP) to enable it to move between cells, usually by increasing the size ... Each segment has one open reading frame (ORF). The first segment, FMV vcRNA 1 (7093 nt), is common to all viruses of genus ... There are still many aspects of this virus' molecular structure and function that are unknown. The protein encoded by the ... Fig mosaic virus (FMV) is a segmented, negative sense, single-stranded RNA virus that is determined to be the causal agent of ...
... encoding the Vif and Rev proteins. An additional short ORF termed orfA (also known as orf2) precedes the env gene. The function ... The capsid protein derived from the polyprotein Gag is assembled into a viral core (the protein shell of a virus) and the ... FIV differs taxonomically from two other feline retroviruses, feline leukemia virus (FeLV) and feline foamy virus (FFV), and is ... The FIV virus genome is diploid. It consists of two identical single-strands of RNA in each case about 9400 nucleotides ...
ORF 2 has some homology to the viral protein 2 of chicken anaemia virus. ORF 3 has no homology to any known protein. Similarly ... ORF 1, 2, 3) and two others (ORF 4 and 5) may also be present. The genome is of negative polarity. ORF 1 possesses a putative ... neither ORF 4 or 5 have any homology with any known protein. The functions of these proteins is not known. A TATA box (sequence ... It has a low level of homology to the Circovirus chicken anemia virus. The virus can be isolated from the blood and all the ...
R1, produced from ORF 3, is translated into non-structural protein 1 (NS1). mRNA transcript R2, produces non-structural protein ... The Minute Virus of Mice (MVM) is the prototype virus of the Protoparvovirus genus within the Parvoviridae family of viruses. ... The resulting NS2 protein is thus composed of spliced 5-prime and 3-prime alternative ORF sections of NS1. Finally, the R3 ... Eichwald, V.; Daeffler, L.; Klein, M.; Rommelaere, J.; Salome, N. (2002). "The NS2 Proteins of Parvovirus Minute Virus of Mice ...
"Structure and Function of Bluetongue Virus and its Proteins". Segmented Double-stranded RNA Viruses: Structure and Molecular ... In most cases each gene segment encodes a single open reading frame (ORF). The genome encodes 7 major structural proteins (VP1- ... Changuinola virus Irituia virus Jamanxi virus Jari virus Gurupi virus Monte Dourado virus Ourem virus Purus virus Saraca virus ... virus Great Island virus Ieri virus Lebombo virus Orungo virus Palyam virus Peruvian horse sickness virus St Croix River virus ...
DNA β has a single open reading frame (βC1 ORF), which encodes a suppressor of RNA silencing. DNA 1 is homologous to the DNA-R ... component of the nanoviruses and encodes a master replication initiator (M-Rep) protein but its presence does not alter symptom ... Cotton leaf curl virus (CLCuV) is a plant pathogenic virus of the family Geminiviridae. In Asia and Africa the major disease of ... Cotton leaf curl virus is distinct from cotton leaf crumple virus. Department of Plant Pathology, University of Arizona. ...
Perez M, Watanabe M, Whitt MA, de la Torre JC (August 2001). "N-terminal domain of Borna disease virus G (p56) protein is ... P24 is encoded by open reading frame II (ORF-II) and undergoes high rates of mutation in humans. It [binds amphoterin-HMGB1, a ... It is a linear negative-sense single stranded RNA virus in the order Mononegavirales. Several of the proteins encoded by the ... The ancestral virus seems likely to have been a high AT content virus. Borna disease was first described in 1885 as "heated ...
The first ORF encodes for nonstructural proteins, while the second ORF encodes the structural proteins like E1 and E2. It is ... MIDV is classified as an Old World Alphavirus which also includes Semliki Forest virus (SFV), Ndumu virus, Barmah Forest virus ... These include: Eastern equine encephalitis (EEE) virus, and Venezuelan equine encephalitis virus. These viruses are more ... The sgRNA codes for the structural proteins that will form the new virus. The assembly of the new viral capsid occurs in the ...
The fourth ORF codes for another cysteine-rich protein that increases virulence and has some RNA silencing suppressive activity ... The other two molecules code for a polymerase and a coat protein both of which are necessary for the survival of the virus. RNA ... After replicates of the viral RNA and of the capsid proteins are made in the cytosol, the virus reassembles itself and exits ... Potato mop-top virus (PMTV) is a plant pathogenic virus transmitted through the vector Spongospora subterranea that affects ...
ORF). The ORF Complimentary 1 (C1) contains the code for the replication initiator protein (Rep) which is responsible for ... Beet curly top virus (BCTV) was first discovered in 1888 in the Western parts of the United States. The virus wasn't fully ... Beet curly top virus (BCTV) is a pathogenic plant virus of the family Geminiviridae, containing a single-stranded DNA. The ... Even though the leafhopper is infected with the virus, the virus does not replicate within the leafhopper and causes no harm to ...
Each ORF's length in base pairs, weight in kilodaltons, and the proteins/enzymes for which it codes are in the table below. The ... "A New Grapevine Virus Discovered by Deep Sequencing of Virus- and Viroid-Derived Small RNAs in Cv Pinot Gris". Virus Research, ... the genome was considered completely novel and the virus officially named Grapevine Pinot gris virus. The virus was recognized ... Apricot pseudo-chlorotic leaf spot virus, Cherry mottle leaf virus, and peach mosaic virus. Giampetruzzi et al. originally ...
In HIV-1, the gag ORF that encodes the 55 kDa Gag protein, the major viral structural protein, is located at the 5' end of the ... However, a shift in the ORF is not universally deleterious, as many viruses capitalize on this phenomenon by using a programmed ... Intact and consistent protein biosynthesis relies on the ability of the ribosome to stay in the correct open reading frame (ORF ... The pol ORF is located 3' to the gag ORF and encodes the Pol polyprotein, which is eventually cleaved into the viral enzymatic ...
8 (1): e2636 Dai, Lianpan (11 May 2016). "Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus ... The RNA genome contains 5' and 3' untranslated regions that surround a single open reading frame (ORF) that encodes for a ... Due to its phylogeny, it is related to the dengue virus, yellow fever virus, Japanese encephalitis virus, and West Nile virus. ... Spondweni virus (SPOV) is an arbovirus, or arthropod-borne virus, which is a member of the family Flaviviridae and the genus ...
... and an RNA virus (tombusvirus). The genome is circular and encodes two proteins-a Rep protein and a capsid protein. Further ... ORF) in opposite orientations. This type is characteristic of the circoviruses, geminiviruses and nanoviruses. Type II genomes ... DNA viruses. A group of ssDNA viruses whose Rep proteins show homology to ssDNA viruses from the families Geminiviridae, ... Other viruses Based on the analysis of the coat protein, Sulfolobus turreted icosahedral virus may share a common ancestry with ...
The red fluorescent protein was quickly observed in mosquito cells and unseen in vertebrate cells. The Eilat virus is unable to ... which encompasses two ORF's (Open Reading Frame which is an incessant stretch of codons that do not contain a stop codon). Four ... related viruses (by super infection exclusion/ Homologous interference). This in turn will allow the virus to form a virus ,(of ... This exclusive virion (a thorough virus which is made of an RNA or DNA core with a protein coat), is found in the Negev desert ...
In the later 1990s the elucidation of the crystal structure of CrPV showed that while the conformation of its capsid proteins ... Picornavirus genomes contain only a single open reading frame (ORF) which is translated into a single polyprotein, but CrPV, as ... A related virus from Arkansas, initially called Pseudoplusia includens virus and redesignated CrPVark, was recorded in the mid- ... Advances in Virus Research 26: 117-141. Scotti, P.D. (1985). The estimation of virus density in isopycnic cesium chloride ...
In order to replicate, poxviruses produce a variety of specialized proteins not produced by other DNA viruses, the most ... Variola virus Smallpox was caused by infection with Variola virus, which belongs to the genus Orthopoxvirus, the family ... While the Dryvax virus was cultured in the skin of calves and freeze-dried, ACAM2000s virus is cultured in kidney epithelial ... During the 19th century, the cowpox virus used for smallpox vaccination was replaced by vaccinia virus. Vaccinia is in the same ...
GIF was produced in vivo during orf virus reinfection. GIF was detected in skin, localised to the area of orf virus infected ... was isolated and mapped to the right terminal quarter of the orf virus genome. The orf virus GIF cDNA was expressed as a ... The characterisation of a GM-CSF and IL-2 inhibitory protein encoded by Orf virus ... Although the vaccinia virus A41L protein has sequence similarity to the T1 secreted chemokine-binding proteins of ...
Ab91273 is an active full length protein produced in Escherichia coli and has been validated in WB… ... Buy our Recombinant Hepatitis B Virus E Antigen protein. ... Precore protein. *Precore/core. *Precore/core ORF. *Precore/ ... Unlike the surface antigen, the e-antigen is found in the blood only when there are viruses also present. When the virus goes ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
Large DNA viruses such as orf virus express an astonishing array of proteins that manipulate responses to infection, including ... Large DNA viruses such as orf virus express an astonishing array of proteins that manipulate responses to infection, including ... Large DNA viruses such as orf virus express an astonishing array of proteins that manipulate responses to infection, including ... Orf virus encodes a unique repertoire of these proteins, which provide an exciting source of new therapeutics with potential ...
2015 Aug;34(4):233-45 Authors: Wang X, Zhang J, Hao W, Peng Y, Li H, Li W, Li M, Luo S Abstract Orf is caused by the orf virus ... Isolation and Characterization of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential ... Diagnostic Tool for Orf Viruses. Monoclon Antib Immunodiagn Immunother. ... of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential Diagnostic Tool for Orf Viruses. ...
Ready to use Zika virus Envelope protein E ORF in plasmid DNA clone, AAV or adenovirus formats. Use individually or as a group ... Zika Virus ORF products: Envelope protein E Description. Envelope protein E of Zika virus, packaged and ready to ship as ... envelope protein E of Zika virus in adenovirus format ZV-E-AD. 100 ul, 1X1010 VP/ml. $499.00 ... envelope protein E of Zika virus in AAV9. ZV-E-AAV9. 100 ul, 1X1013 GC/ml. $499.00 ...
Orf Virus) (QP10912). Host: E. Coli. Available Tags: His. Purity: Greater than 90.0% as determined by SDS-PAGE.. From: 89. ... Orf Virus) Bioactive Protein Product Attributes. Product Type: Bioactive Protein. Recombinant VEGF E (Orf Virus) based upon ... Be the first to review "Recombinant Virus VEGF E (Orf Virus) Protein" Cancel reply. Your email address will not be published. ... Protein Construction: A cDNA sequence encoding the sequence of VEGF E (Orf Virus) was constructred and used to recombinantly ...
Here it is demonstrated that the five orf virus (ORFV) ANK repeat proteins ov008, ov123, ov126, ov128, and ov129 interact with ... Cite this item: Chen, D.-Y. (2015). The Functional Analyses of Ankyrin Repeat Proteins of Orf Virus (Thesis, Doctor of ... The Functional Analyses of Ankyrin Repeat Proteins of Orf Virus. Chen, Da-Yuan ... Viruses, especially the Poxviridae and Polydnaviridae families, also encode ANK repeat containing proteins. ANK repeat proteins ...
AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a ... 7. Why is the SDS-Page blot of the protein at a higher MW than what I expected to see? Yes, the final size of a natural protein ... Vascular Endothelial Growth Factor-E Recombinant (Orf Virus) Physical Activity. Sterile Filtered White lyophilized (freeze- ... The lyophilized Vascular Endothelial Growth Factor-E (Orf Virus) should be reconstituted in PBS or medium containing at least ...
The chemokine-binding protein from orf virus was purified and crystallized. The morphology and diffraction behaviour of these ... Crystallization and preliminary X-ray analysis of the chemokine-binding protein from orf virus (Poxviridae). ... T. cruzi TcNDPK1 was overexpressed in Escherichia coli as an N--terminally poly-His-tagged fusion protein and crystallized. ... The crystal structure of smu.1377c, a hypothetical protein from S. mutans, shows a similar fold to Sua5_YciO_YrdC-family ...
Enhance Green Fluorescent ProteinRecombinant VirusWild Type VirusEnhance Green Fluorescent Protein ExpressionLimited Dilution ... Generation of recombinant Orfvirus using an enhanced green fluorescent protein reporter gene as a selectable marker. ... Haig DM, Fleming S: Immunomodulation by virulence proteins of the parapoxvirus orf virus. Vet Immunol Immunopathol. 1999, 72: ... we developed a rapid method to generate recombinant Orf viruses (ORFV) based on the enhanced green fluorescent protein (EGFP) ...
Over 37,000 human full-length ORF cDNA clones for gene function study. ... Virus (10) * Canine (9) * Goat (7) * High Gc Gram+ (5) * Orf Virus (5) ... Recombinant protein of human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR) ... Purified recombinant protein of human S100 calcium binding protein A9 (S100A9), with C-terminal MYC/DDK tag, secretory ...
Over 37,000 human full-length ORF cDNA clones for gene function study. ... Orf Virus * Ascomycetes * Schistosoma * Streptococcus * Streptococcus Pyogenes * Protozoan * A. victoria * Apicomplexan * ... Recombinant protein of human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR) ... Purified recombinant protein of human S100 calcium binding protein A9 (S100A9), with C-terminal MYC/DDK tag, secretory ...
In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex ... which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. ... depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and ... SKP1-CUL1-F-box protein) ubiquitin ligase complex, ... From Orf virus (strain Goat/Texas/SA00/2000).. Q6TVJ4. 2. EBI- ...
Vaccinia virus ORF library: Expression library that contains each of the predicted open reading frames for vaccinia virus - ... Fluorescent Protein Guide: Addgenes extensive fluorescent protein plasmid collection is organized by application and by color. ... Fluorescent Proteins. *FRET-based biosensors: E. coli expression - Pertz Lab. *Bacterial Fluorescence Labeling: Broad-Range ... EcoFlex MoClo: Modular cloning for applications like recombinant protein purification and cell-free circuit design in E. coli ...
Protein Coding), Mucin 6, Oligomeric Mucus/Gel-Forming, including: function, proteins, disorders, pathways, orthologs, and ... MUC6 CRISPR as ready-to-use vector or virus: ORF , Lenti- , Adeno- , AAV- , Protein Vector - Browse All ... Protein details for MUC6 Gene (UniProtKB/Swiss-Prot). Protein Symbol:. Q6W4X9-MUC6_HUMAN. Recommended name:. Mucin-6. Protein ... MUC6 as ready-to-use vector or virus: ORF , Lenti- , Retro- , Adeno- , AAV- , Protein Vector - Browse All ...
Protein Coding), Chromosome 1 Open Reading Frame 68, including: function, proteins, disorders, pathways, orthologs, and ... C1orf68 CRISPR as ready-to-use vector or virus: ORF , Lenti- , Adeno- , AAV- , Protein Vector - Browse All ... C1orf68 as ready-to-use vector or virus: ORF , Lenti- , Retro- , Adeno- , AAV- , Protein Vector - Browse All ... C1orf68 CRISPR as ready-to-use vector or virus: ORF , Lenti- , Adeno- , AAV- , Protein Vector - Browse All ...
Protein Coding), S100 Calcium Binding Protein B, including: function, proteins, disorders, pathways, orthologs, and expression ... S100B CRISPR as ready-to-use vector or virus: ORF , Lenti- , Adeno- , AAV- , Protein Vector - Browse All ... Protein Symbol:. P04271-S100B_HUMAN. Recommended name:. Protein S100-B. Protein Accession:. P04271. Secondary Accessions: * ... S100B as ready-to-use vector or virus: ORF , Lenti- , Retro- , Adeno- , AAV- , Protein Vector - Browse All ...
Protein Coding), Guided Entry Of Tail-Anchored Proteins Factor 4, including: function, proteins, disorders, pathways, orthologs ... GET4 CRISPR as ready-to-use vector or virus: ORF , Lenti- , Adeno- , AAV- , Protein Vector - Browse All ... GET4 as ready-to-use vector or virus: ORF , Lenti- , Retro- , Adeno- , AAV- , Protein Vector - Browse All ... GET4 CRISPR as ready-to-use vector or virus: ORF , Lenti- , Adeno- , AAV- , Protein Vector - Browse All ...
Orf can be transmitted to humans by contact with an infected animal or contaminated fomites. ... Orf is a viral disease that is widespread in sheep and goats. ... Antibodies Against a Virion Core Protein of Orf Virus Strain ... The orf virus is a cylindrical virus measuring 260 X 160 nm. Its surface tubules form a long crisscross design that is seen on ... Orf is caused by infection with the orf virus that belongs to the Parapoxvirus genus, which also includes the milkers nodule ...
... the left ORF encodes the nonstructural protein NS1 and the right ORF encodes three capsid proteins . One of the three ... Among the more common poxviruses are modified vaccinia virus Ankara (MVA), fowlpox virus, and orf virus. MVA has been a ... SIV/PRV Recombinant Virus Vaccine. Swine influenza virus (SIV) is a type A virus, which is enveloped and consists of negative ... "Functional roles of the tegument proteins of herpes simplex virus type 1," Virus Research, vol. 145, no. 2, pp. 173-186, 2009. ...
... recombinant proteins and synthetic proteins, as well as peptides, lysates and more. ... Cell Sciences carries an extensive range of native proteins, ... Recombinant Orf Virus VEGF-E, His-Tag. 5 μg. $380.00 BUY ... Recombinant Human Heparin Binding Domain VEGFA/ Orf Virus VEGFE. 20 μg. $640.00 BUY ... Recombinant Human Heparin Binding Domain VEGFA/ Orf Virus VEGFE. 5 μg. $320.00 BUY ...
Cloning of the Nipah virus P ORF and truncations thereof.Nipah virus cDNA of the P open reading frame (ORF) was constructed by ... the VP35 protein of Ebola virus (4), the NSs protein of Bunyamwera virus (58), and the ML protein of Thogoto virus (23). Within ... The Nipah virus P protein has IFN antagonist activity.The Nipah virus P gene is predicted to encode four proteins: the ... The Nipah virus P protein has IFN antagonist activity. (A) Schematic representation of the Nipah virus V, W, and P proteins, ...
This virus had a unique insertion in the NS4A gene. However, the envelope protein contained Lys 84, which was specific to ... Whole-genome analysis showed 90.4% identity with other genotype 5 viruses from humans. ... We isolated Japanese encephalitis virus genotype 5 from human specimens in South Korea. ... We compared the entire ORF sequences of K15P38 virus with previously reported strains of JEV genotypes 1-5. Phylogenetic ...
Orf virus (1) * P. falciparum (1) * Peach (3) * Pedobacter heparinus (1) * Plant (1) ... are chromatin-associated proteins involved in gene regulation and heterochromatin formation. CBX proteins have an N-terminal ... The heterochromatin protein 1 (HP1) family, which consists of isoforms HP1-alpha (CBX5), HP1-beta (CBX1), and HP1-gamma (CBX3 ... PHD fingers are zinc binding domains that have a Cys4-His-Cys3 motif and are found in more than 100 nuclear proteins that play ...
Mitochondrial targeting of the p13II protein coded by the x-II ORF of human T-cell leukemia/lymphotropic virus type I (HTLV-I) ... protein coded by the x-II ORF of human T-cell leukemia/lymphotropic virus type I (HTLV-I) . Opens in a new window. ... Inhibition of Tunneling Nanotube (TNT) Formation and Human T-cell Leukemia Virus Type 1 (HTLV-1) Transmission by Cytarabine * ... Smallpox vaccine-induced antibodies are necessary and sufficient for protection against monkeypox virus *Yvette Edghill-Smith ...
Capsid proteinVacciniaAntibodiesMammalianNucleotideSingle-strandedRecombinant ProteinKinaseInfectionsAccessory proteinsNonstructuralExpressionVaccineGenomicInfluenza A virImmunogenicVirally encoded proteinsPeptidesORFsMRNAVirologyCellularVirionsVivoFunctionalInteractAntigensSimilarityMotifPolyproteinCharacterizationMembrane-spanninSequence homologyGenusViral geneOpen ReadiSolubleSpeciesVirulenceNucleocapsidGranulocyte macropAntigenAntibodyColiHerpesEnvelope
- Structural analysis of the major capsid protein has suggested an evolutionary link between archaeal viruses and bacterial and eukaryotic viruses [ 7 , 15 ]. (hindawi.com)
- These data indicate that 415R is a potential matrix protein connecting the envelope protein 118L with the major capsid protein 274L. (wur.nl)
- The CaMV particle is an icosahedron with a diameter of 52 nm built from 420 capsid protein (CP) subunits arranged with a triangulation T = 7, which surrounds a solvent-filled central cavity. (wikipedia.org)
- The host humoral response against the capsid protein represents the most active immune pressure and primary weapon to control the infection. (frontiersin.org)
- The VP1 capsid protein contains a unique (VP1u) phospholipase A2 domain (PLA2) and two nuclear localization signals. (ufl.edu)
- The GIF gene encodes a 28 kDa protein that exhibits 32% amino acid sequence similarity to the predicted sequence of the A41L gene product encoded by vaccinia virus. (bl.uk)
- Although the vaccinia virus A41L protein has sequence similarity to the T1 secreted chemokine-binding proteins of leporipoxviruses, its function is not known. (bl.uk)
- The coding sequence of EGFP gene was amplified from pEGFP-N1 vector and subcloned into the pZIPPY-neo/gus plasmid under the control of the early-late vaccinia virus (VACV) VV7.5 promoter and flanked by two multiple cloning sites (MCS) to generate a novel transfer vector pSPV-EGFP. (beds.ac.uk)
- However, the experimental procedures to generate and isolate rORFVs are adapted from standard protocols used in generation of the vaccinia virus. (beds.ac.uk)
- Inhibition of apoptosis and NF-κB activation by vaccinia protein N1 occur via distinct binding surfaces and make different contributions to virulence. (biomedsearch.com)
- Vaccinia virus (VACV) protein N1 is an intracellular virulence factor and belongs to a family of VACV B-cell lymphoma (Bcl)-2-like proteins whose members inhibit apoptosis or activation of pro-inflammatory transcription factors, such as interferon (IFN) regulatory factor-3 (IRF-3) and nuclear factor-κB (NF-κB). (biomedsearch.com)
- Vaccinia virus E3L encodes a dsRNA binding protein that inhibits PKR in virus-infected cells, presumably by sequestering dsRNA activators. (asm.org)
- The vaccinia virus E3L product is a dsRNA-binding protein capable of inhibiting PKR by sequestering dsRNA activators ( 3 , 7 , 26 , 49 ). (asm.org)
- Rabbit antisera raised against a synthetic peptide from the C terminus of the j13L ORF identified proteins of 25-27 kDa in cells infected with a recombinant vaccinia virus expressing the j13L ORF, in ASFV-infected cells and in purified extracellular ASF virions. (nih.gov)
- Although ASFV-infected animals made antibodies to the j13L protein, no protection was observed when pigs were vaccinated with a recombinant vaccinia virus expressing the j13L ORF. (nih.gov)
- However, unlike most poxviruses, MCV lacks a homologue to the E3L and K3L proteins of vaccinia virus, which are involved in the control of the key antiviral and pro-apoptotic dsRNA-dependent protein kinase, PKR. (microbiologyresearch.org)
- Isolation and Characterization of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential Diagnostic Tool for Orf Viruses. (medworm.com)
- These hybridomas were used to produce antibodies targeting ORFV proteins. (medworm.com)
- Sera from stallions persistently infected with EAV and horses immunized repeatedly with the modified live EAV vaccine contained antibodies specific for the GP3 protein. (nih.gov)
- Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
- This HEV-Ab ELISA kit is an enzyme linked-immunosorbent assay for in vitro qualitative detection of total antibodies (IgG, IgM,etc.) to hepatitis E virus in serum or plasma. (rapidtest.com)
- Consistent with this, some mammalian ANK repeat containing proteins have been shown to interact with FIH-1 and undergo asparaginyl hydroxylation. (otago.ac.nz)
- Yes, the final size of a natural protein, produced in mammalian cells under normal circumstances is at the molecular weight reported in literature. (promab.com)
- Our portfolio includes recombinant proteins produced in mammalian, insect and bacterial cell expression systems. (cellsciences.com)
- 6) The protein encoded by this avian polyomavirus ORF has no apparent sequence homology to simian virus 40 VP4 or the mammalian polyomavirus agnoprotein. (thefreelibrary.com)
- There are three classes of mammalian Arf proteins, distinguished by size and homology ( 1 ). (bioscience.org)
- Mammalian PKR is a double-stranded RNA (dsRNA)-dependent protein kinase that is transcriptionally induced by interferon and becomes activated in virus-infected cells by dsRNAs produced during the virus life cycle. (asm.org)
- Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
- These factors include a homologue of mammalian interleukin (IL)-10, a chemokine binding protein and a granulocyte-macrophage colony stimulating factor /IL-2 binding protein. (springer.com)
- The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. (uniprot.org)
- In recent years, nucleotide and amino acid sequence information has become available so that additional characterization of these viruses is possible, including further analysis of relationships among members of the genus. (cdc.gov)
- Deletion of the nucleotide sequence encoding the PEST sequence between the two initiation codons unusually reduced the protein molecular size on SDS-polyacrylamide gel-electrophoresis. (scirp.org)
- During attenuation of PRV by serial passaging in Vero cells, dynamic variation patterns including a large deletion, single nucleotide variations, small in-frame indels, and also frameshifts mutations successively emerged, contributing to evolution of the viral population and enabling the gradual attenuation of the virus. (springer.com)
- This cycle is mediated by guanine nucleotide exchange factors (GEFs) and guanine nucleotide activating proteins (GAPs) known as Arf GEFs and Arf GAPs. (bioscience.org)
- PKR interferes with virus propagation by phosphorylating the α subunit of translation initiation factor 2 (eIF2α), converting eIF2 from a substrate to an inhibitor of its guanine nucleotide exchange factor, eIF2B. (asm.org)
- GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN #=GF RM 1926776 #=GF RT Complete nucleotide sequence of the virus SSV1 of the #=GF RT archaebacterium Sulfolobus shibatae. (genome.jp)
- We also observed inter-strand nucleotide substitution imbalances that are consistent with a recent proposal that high mutation rates in geminiviruses (and possibly ssDNA viruses in general) may be due to mutagenic processes acting specifically on ssDNA molecules. (ufl.edu)
- 11-13) Similar to other single-stranded DNA viruses, BFDV is prone to a high rate of genetic mutation, although the rep gene is relatively conserved. (thefreelibrary.com)
- Panicum mosaic virus (PMV) is a positive-sense single-stranded RNA viral pathogen that infects plant species in the Panicoid tribe of the grass family Poaceae. (wikipedia.org)
- Members of Tombusviridae are transmitted as positive sense single-stranded non-enveloped RNA viruses, with an icosohedral capsid. (wikipedia.org)
- Alphasatellites are single stranded satellite DNA that are dependent on a virus for transmission. (wikipedia.org)
- Picornaviruses are small naked RNA-positive single-stranded viruses that include some of the most important pillars in the development of virology, comprising poliovirus, rhinovirus, and hepatitis A virus. (frontiersin.org)
- Background: Recent reports have indicated that single-stranded DNA (ssDNA) viruses in the taxonomic families Geminiviridae, Parvoviridae and Anellovirus may be evolving at rates of ~10-4 substitutions per site per year (subs/site/year). (ufl.edu)
- Intriguingly, and possibly contra- dicting the premise that polymerase fidelity is the major universal determinant of evolution rates, figures closer to those of RNA viruses (~10-4 subs/site/year) have been reported for the small single stranded DNA (ssDNA) anelloviruses [7-and parvoviruses [10- (ufl.edu)
- Porcine circoviruses (PCVs), classified as a member of the family Circoviridae, are small icosahedral non-enveloped viruses (size ~17 nm) containing a circular single-stranded DNA molecule of about 1.7 kb. (pubmedcentralcanada.ca)
- Class VI: Positive-sense single-stranded RNA viruses that replicate through a DNA intermediate. (kenyon.edu)
- Identified in 1990, Hepatitis E (HEV) is a non-enveloped, single stranded RNA virus where infection can lead to acute (or subclinical) liver disease, like that associated to hepatitis A infection. (rapidtest.com)
- Adeno-Associated Viruses (AAVs) are single stranded DNA viruses that belong to the Parvoviridae family. (ufl.edu)
- However, we are making a recombinant protein, with 2 tags, and in yeast cells. (promab.com)
- In this work, we cloned the A32L gene into a prokaryotic expression vector, and the recombinant protein was expressed and purified. (deepdyve.com)
- The ATP hydrolysis function of the purified recombinant protein was examined, and our results showed that it exhibited the ATPase activity. (deepdyve.com)
- Among the antiviral proteins induced in response to type I IFN are dsRNA-dependent protein kinase R (PKR), 2′,5′-oligoadenylate synthetase (OAS), and the Mx proteins ( 7 - 10 ). (pnas.org)
- The human double-stranded RNA (dsRNA)-dependent protein kinase PKR inhibits protein synthesis by phosphorylating translation initiation factor 2α (eIF2α). (asm.org)
- We propose that effective inhibition of PKR in yeast requires formation of an E3-PKR-dsRNA complex, in which the N-terminal half of E3 physically interacts with the protein kinase domain of PKR. (asm.org)
- 7,11,Orf B has been shown to interact with the protein receptor for activated C kinase (RACK1). (kenyon.edu)
- RACK1 binds to some activated isoforms of protein kinase C (PKC), stabilizing PKC at the membrane in an active conformation. (kenyon.edu)
- Activation of NF-κB via the IL-1 receptor and TLRs requires the recruitment of the adaptor proteins myeloid differentiation factor 88 (MyD88) and TNF-receptor-associated factor 6 (TRAF6), whereas activation via the TNF receptor requires the kinase receptor interacting protein (RIP) and TRAF2 , . (prolekare.cz)
- The majority of orf infections go unreported because the disease is self-limited, and those infected are able to recognize orf and do not seek medical attention. (medscape.com)
- A lentiviral vector-based, herpes simplex virus 1 (HSV-1) glycoprotein B vaccine affords cross-protection against HSV-1 and HSV-2 genital infections. (nih.gov)
- The data indicate that the GP3 protein is an extensively glycosylated membrane protein that is immunogenic during some EAV infections. (nih.gov)
- Hepatitis B e-antigen (HBeAg) is a viral protein associated with HBV infections. (abcam.com)
- Fv1 appears to represent an ancient retroviral gag protein co-opted by the host cell to prevent viral infections ( 2 , 3 ). (asm.org)
- Ebola virus infections frequently result in severe hemorrhagic fever, and epidemics of the Ebola virus, Zaire subtype have resulted in mortality rates of greater than 80% ( 1 , 2 ). (pnas.org)
- The pathologic features and the immune responses characteristic of fatal and nonfatal human Ebola virus infections have begun to be characterized ( 3 - 5 ). (pnas.org)
- To fully understand the pathogenesis of Ebola virus infections, it will be important to study further the mechanisms by which the virus interacts with its host, including the ways in which the virus subverts the host antiviral response. (pnas.org)
- One outcome of many virus infections is the regulation of cellular trafficking machinery. (bioscience.org)
- Picornaviridae is a large family of vertebrate viruses that produce both clinically asymptomatic infections but often mild and fatal disease. (frontiersin.org)
- Moreover, compelling evidence indicates that the worldwide-distributed prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a neglected human pathogen of clinical significance ( 23 , 32 ) and poses a special threat to immunocompromised individuals, as illustrated by cases of transplant-associated infections by LCMV with fatal outcomes ( 10 , 36 , 40 ). (pubmedcentralcanada.ca)
- These findings demonstrate the potential of eleutheroside B1 for use in the prevention and treatment of influenza A virus‑mediated infections. (spandidos-publications.com)
- Therapeutic paint of Cidofovir/Sucralfate gel combination topically administered by spraying for treatment of orf virus Infections The AAPS Journal 11(2):242 - 249. (moredun.org.uk)
- Immune responses directed against cryptic epitopes have been detected in several infections, including influenza virus ( 12 ), murine AIDS ( 13 ), and, importantly, HIV ( 14 ). (rupress.org)
- The IFN antagonist functions of members of the subfamily Paramyxovirinae are all encoded by the phosphoprotein (P) gene and in most cases are carried out by one of the accessory proteins V and C. These proteins all target the IFN signaling, or JAK/STAT, pathway but do so through distinct mechanisms. (asm.org)
- In addition to known non-structural and structural proteins all coronaviruses have one or more accessory proteins whose functions are mostly unknown. (biomedcentral.com)
- Respiratory syncytial virus (RSV), a member of the subfamily Pneumovirinae , expresses two nonstructural proteins, NS1 and NS2, which for bovine RSV have been shown to act cooperatively to block the IFN response ( 48 ). (asm.org)
- Additionally, an M segment-encoded nonstructural protein, NS M , was recently identified in the nairovirus Crimean-Congo hemorrhagic fever virus (CCHFV) ( 1 ). (cdc.gov)
- The results indicate that 5F2D8 specifically recognizes orf virus encoded protein ORFV086, a late expression virion core protein that plays important roles in progeny virus particle assembly, morphogenesis, and maturity. (medworm.com)
- The charge will be $3,100 for cloning, protein expression services, and purification in E. coli. (promab.com)
- Viruses have devised various strategies to circumvent this IFN-induced antiviral state through the expression of viral proteins that inhibit specific components of the IFN system ( 14 , 29 , 31 ). (asm.org)
- CBX2 is a component of the polycomb repressive complex 1 and represses protein expression. (biomol.com)
- Ankyrin protein AnkB can affect the gene expression of catB , catalase activity, and sensitivity to H 2 O 2 . (asm.org)
- This study is the first extensive expression analysis of a group 1 hCoV-ORF 3 protein. (biomedcentral.com)
- 5. The expression plasmid of claim 1, further comprising a negative strand RNA virus viral gene segment inserted between the pol I promoter and the termination signal. (freepatentsonline.com)
- 6. The expression plasmid of claim 5, wherein the negative strand RNA virus is a member of the Orthomyxoviridae virus family. (freepatentsonline.com)
- 7. The expression plasmid of claim 6, wherein the virus is an influenza A virus. (freepatentsonline.com)
- In this system, the endothelial-specific promoter for intercellular adhesion molecule 2 (ICAM2) was cloned into the dual AAV vectors of SpGuide and SpCas9 for driving expression of green fluorescence protein (GFP) and SpCas9, respectively. (arvojournals.org)
- The mechanism of expression of these proteins is unique, in that the ORF VI protein (encoded by the 19S RNA) controls translation reinitiation of major open reading frames on the polycistronic 35S RNA, a process that normally only happens on bacterial mRNAs. (wikipedia.org)
- Expression of PKR in Saccharomyces cerevisiae inhibits protein synthesis by phosphorylation of eIF2α, dependent on its two dsRNA binding motifs (DRBMs). (asm.org)
- The entire ORF of the UL31 was cloned into pET 32a (+) prokaryotic expression vector. (biomedcentral.com)
- Escherichia coli BL21(DE3) competent cells were transformed with the construct followed by the induction of protein expression by the addition of IPTG. (biomedcentral.com)
- With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
- Expression of virally encoded CXCL10 within the brain protected mice from death and correlated with increased infiltration of T lymphocytes, enhanced IFN-γ secretion, and accelerated viral clearance when compared with mice infected with an isogenic control virus, MHV. (jimmunol.org)
- In this report, a highly soluble Cap-tag protein expressed in E.coli was constructed with a p-SMK expression vector with a fusion tag of small ubiquitin-like modifiers (SUMO). (pubmedcentralcanada.ca)
- Lee, Gung 2016-06-01 00:00:00 Heterologous gene expression using plant virus vectors enables research on host-virus interactions and the production of useful proteins, but the host range of plant viruses limits the practical applications of such vectors. (deepdyve.com)
- The subgenomic promoter (SGP) in the coat protein (CP) gene, which was used to drive heterologous expression, was mapped by analyzing deletion mutants from a CaMV 35S promoter-driven infectious CFMMV clone. (deepdyve.com)
- The TLR-5-specific bioactivity of fusion protein rGP5-FljB was determined by detecting the expression levels of the cytokine IL-8 in HEK293-mTLR5 cells by sandwich ELISA. (biomedcentral.com)
- Recombinant viruses represent a particularly promising avenue of vaccine research both for improving existing vaccines and for developing new ones [ 8 , 9 ]. (hindawi.com)
- This has prompted the need to fully comprehend virus attenuation for the development of new vaccine candidates. (springer.com)
- report that a version of RSV lacking the M2-2 protein can induce immunity despite decreased vaccine virus shedding in young children. (sciencemag.org)
- Respiratory syncytial virus (RSV) is the leading viral cause of severe pediatric respiratory illness, and a safe and effective vaccine for use in infancy and early childhood is needed. (sciencemag.org)
- When results in RSV-seronegative children were compared to those achieved with the previous leading live attenuated RSV candidate vaccine, vaccine virus shedding was significantly more restricted, yet the postvaccination RSV-neutralizing serum antibody achieved [geometric mean titer (GMT) = 1:was significantly greater. (sciencemag.org)
- The capsid (Cap) protein of PCV2 is a major candidate antigen for development of recombinant vaccine and serological diagnostic method. (pubmedcentralcanada.ca)
- The recombinant Cap protein has the ability to self-assemble into virus-like particles (VLPs) in vitro , it is particularly opportunity to develop the PV2 VLPs vaccine in Escherichia coli ,( E.coli ), because where the cost of the vaccine must be weighed against the value of the vaccinated pig, when it was to extend use the VLPs vaccine of PCV2. (pubmedcentralcanada.ca)
- However, high-efficiency transient transfection of a plasmid encoding a protein that interferes with type I IFN-induced antiviral functions, such as the influenza A virus NS1 protein or the herpes simplex virus protein ICP34.5, rescues growth of influenza delNS1 virus. (pnas.org)
- First, the influenza A virus NS1 protein was shown to block IFN responses in virus-infected cells ( 12 ). (pnas.org)
- The open reading frame 3 of equine arteritis virus encodes an immunogenic glycosylated, integral membrane protein. (nih.gov)
- ORF2 encodes the major structural capsid (Cap) protein, which is a sole structural protein of the viral coat [ 5 ] and also the major immunogenic protein that works together with the principal carrier of type-specific epitopes [ 6 ]. (pubmedcentralcanada.ca)
- The Cap protein is highly immunogenic and reacts strongly with the serum of PCV2-infected pigs. (pubmedcentralcanada.ca)
- ORF2 encodes the structural capside-forming protein Cap, the main immunogenic antigen of the virus. (omicsonline.org)
- Proteins and peptides are key research tools for life scientists. (cellsciences.com)
- Cell Sciences offers an extensive range of over 7,000 proteins and peptides. (cellsciences.com)
- Our catalog of proteins and peptides are high purity reagents with validated bioactivity and low endotoxicity. (cellsciences.com)
- CD8-TLs recognize virus-infected cells by engaging complexes of virally derived peptides and MHC-I molecules. (rupress.org)
- The obvious and best-studied source of these peptides is previously described viral proteins. (rupress.org)
- Recent research demonstrated that the F-box-like motif of at least some poxviral ANK proteins is able to bind cellular SKP1 protein, thus the ANK proteins might associate with host cellular ubiquitin ligase complexes. (otago.ac.nz)
- The role of asparaginyl hydroxylation of cellular ANK repeat proteins remains elusive but these observations raised the possibility that viral ANK repeat proteins might interact with FIH-1 in infected cells. (otago.ac.nz)
- S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (genecards.org)
- Lytic viruses of bacteria typically utilize a holin/endolysin-based mechanism for virion release [ 16 , 17 ], whereas virion egress via a cellular budding mechanism is commonly used by enveloped animal viruses. (hindawi.com)
- Env is primarily responsible for binding the cellular receptor and for effecting the fusion process, with these functions mediated by protein domains localized to the exterior of the virus. (mdpi.com)
- Env is the receptor binding protein, facilitating the early steps in the virus-cell interaction and additionally drives the fusion process between the viral and cellular membranes. (mdpi.com)
- Viral proteins recruit and interact with cellular trafficking proteins to divert the normal trafficking of key proteins or to induce the formation of novel membrane structures in the host cell. (bioscience.org)
- Our knowledge of how viruses interact with the cellular trafficking machinery is still limited and furthering this understanding will be important for the future development of prophylactic and therapeutic treatments. (bioscience.org)
- A protein of approximate 35 kDa that reacted with the antiserum was detected in immunoblots of DEV-infected cellular lysates, suggesting that the 35 kDa protein was the primary translation product of the UL31 gene. (biomedcentral.com)
- The discovery of an immunodominant CD8-TL response in elite controller macaques against a cryptic epitope suggests that the AIDS virus-specific cellular immune response is likely far more complex than is generally assumed. (rupress.org)
- Analysis of purified viral particles revealed that ORF 3 protein is incorporated into virions and is therefore an additional structural protein. (biomedcentral.com)
- Once the virus has successfully invaded the plant, panicum mosaic virions spread through the plant by producing three proteins (p8, p6.6, and p15) as part of their capsids. (wikipedia.org)
- Members of the family Filoviridae (filoviruses) are non-segmented negative-strand RNA viruses that produce filamentous enveloped virions. (mdpi.com)
- These virions contain a single virus‑encoded membrane glycoprotein, GP, which is organized into homotrimeric spikes on the viral surface (see [ 7 , 8 , 9 ] for recent reviews). (mdpi.com)
- GIF was produced in vivo during orf virus reinfection. (bl.uk)
- This product is an active protein and may elicit a biological response in vivo, handle with caution. (abcam.com)
- Furthermore, in vivo experiments with ducks infected with UL31-defective isolates of DEV will also be of importance in order to assess the possible role of the UL31 protein in viral pathogenesis. (biomedcentral.com)
- The crystal structure of smu.1377c, a hypothetical protein from S. mutans , shows a similar fold to Sua5_YciO_YrdC-family proteins and indicates its functional role in tRNA modification. (iucr.org)
- The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. (uniprot.org)
- Hence, all three reading frames are used to produce functional proteins. (nature.com)
- Despite these genetic and functional differences, however, the C -terminal domains of these viruses share a common feature in the modulation of Env ectodomain conformation. (mdpi.com)
- This conclusion was supported by the fact that E3 activity was antagonized, not augmented, by overexpressing the catalytically defective PKR-K296R protein containing functional DRBMs. (asm.org)
- Ho CH, Tsai SF (2017) Functional and biochemical characterization of a T cell-associated anti-apoptotic protein, GIMAP6. (phosphosite.org)
- These properties of the UL31 protein provide a prerequisite for further functional analysis of this gene. (biomedcentral.com)
- Cells induced to transiently express Orf C exhibited apoptotic morphology, implying a functional role for WDSV Orf C in an alteration of mitochondrial function that results in apoptosis contributing to tumor regression. (kenyon.edu)
- One application of the system is production of attenuated, reassortant influenza viruses for use as antigens in vaccines. (freepatentsonline.com)
- This HEV-Ab ELISA kit uses polystyrene microwell strips pre-coated with recombinant HEV antigens (HEVAg) corresponding to structural proteins ORF-2 of the native virus. (rapidtest.com)
- Nearly 80% of these viral proteins carry an F-box-like motif near the C-terminus. (otago.ac.nz)
- PHD fingers are zinc binding domains that have a Cys4-His-Cys3 motif and are found in more than 100 nuclear proteins that play a role in regulating chromatin. (biomol.com)
- We herein show that clover yellow vein virus (ClYVV) produces a previously unidentified factor, P3N-ALT, in the +1 reading frame via transcriptional slippage at a conserved G 1-2 A 6-7 motif, as is the case for P3N-PIPO. (nature.com)
- All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al. (reliatech.de)
- PKR is activated by dsRNA in vitro, and the N-terminal half of the protein contains two copies of a dsRNA binding motif (DRBM) also present in other dsRNA binding proteins (reviewed in references 11 and 33 ). (asm.org)
- we have, for the first time, utilized the SUMO fusion motif to successfully express the entire authentic Cap protein of PCV2 in E. coli . (pubmedcentralcanada.ca)
- This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. (fishersci.com)
- An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro. (antikoerper-online.de)
- Japanese encephalitis is caused by Japanese encephalitis virus (JEV), a mosquitoborne virus of the family Flaviviridae , genus Flavivirus ( 1 ). (cdc.gov)
- We have previously described isolation and preliminary identification of a virus related to Dugbe virus (DUGV), family Bunyaviridae , genus Nairovirus . (cdc.gov)
- We suggest that this is a new virus in the family Bunyaviridae , genus Nairovirus , and we propose that it be designated Kupe virus. (cdc.gov)
- The genus Nairovirus in the family Bunyaviridae comprises 7 species groups containing primarily tick-borne viruses, some of which have been identified as human or animal pathogens. (cdc.gov)
- Our findings suggest that this is a new virus in the genus Nairovirus , and we propose that it be designated Kupe virus (Kupe is the Kiswahili word for tick). (cdc.gov)
- Aves polyomavirus 1 (APyV), formerly known as budgerigar fledgling disease virus, is a double-stranded DNA virus and a member of the family Polyomaviridae (genus Gammapolyomcivirus). (thefreelibrary.com)
- Panicum mosaic virus is the type species of the genus Panicovirus, a member of the plant virus family Tombusviridae. (wikipedia.org)
- PMV was placed in the genus Panicovirus because of its limited monocot host range and lack of an additional 5'-ORF. (wikipedia.org)
- Cauliflower mosaic virus (CaMV) is a member of the genus Caulimovirus, one of the six genera in the Caulimoviridae family, which are pararetroviruses that infect plants. (wikipedia.org)
- The Zika virus is transmitted to people through the bite of an infected mosquito from the Aedes genus, mainly Aedes aegypti in tropical and subtropical regions and Aedes albopictus in temperate climates. (elsevier.es)
- Mosquitoes, primarily from the genus Culex, become infected by feeding on birds infected with the Saint Louis encephalitis virus. (wikipedia.org)
- The genus Epsilonretrovirus includes three species of piscine retroviruses, WDSV and walleye epidermal hyperplasia virus (WEHV) types I and II. (kenyon.edu)
- Here, we aimed to develop a viral vector based on cucumber fruit mottle mosaic virus (CFMMV), a member of the genus Tobamovirus , whose members infect cucurbits. (deepdyve.com)
- The outbreak occurred in an area close to a region where the presence of Tobacco streak virus (TSV), family Bromoviridae , genus Ilarvirus , is historical. (scielo.br)
- 1955). At that time, the authors mentioned that similar symptoms were described in soybean in the USA, but these symptoms were caused by Tobacco ringspot virus (TRSV), family Comoviridae , genus Nepovirus . (scielo.br)
- C1orf68 (Chromosome 1 Open Reading Frame 68) is a Protein Coding gene. (genecards.org)
- Our study focuses on hCoV-NL63 open reading frame 3 (ORF 3) which is a highly conserved accessory protein among coronaviruses. (biomedcentral.com)
- ]. Open reading frame III, which previously had no assigned function and is a minor coding region, is believed to promote DNA binding as well as produce structural proteins. (kenyon.edu)
- The second minor coding region, open reading frame VII, has an unknown function but is speculated to have some function relating to targeting new viruses and bringing them to inclusion bodies (P, Ashwathi. (kenyon.edu)
- The region from nucleotides (nt) −55 to +160 relative to the start codon of the open reading frame (ORF) of CP was found to be a fully active promoter, and the region from nt −55 to +100 was identified as the active core promoter. (deepdyve.com)
- In most of cases, the protein will be purified from inclusion body and the activity has not been tested although the soluble form can be obtained sometimes. (promab.com)
- As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). (genecards.org)
- It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303). (genecards.org)
- Highlights of our broad range of recombinant human proteins include our top selling human soluble VEGFR1/FLT1 and human FGF-21 . (cellsciences.com)
- The EAV GP3 protein was membrane associated and not released as a soluble protein, in marked contrast to the ORF 3-encoded proteins of some other arteriviruses. (nih.gov)
- We also offer animal-free versions of recombinant proteins and native proteins purified from tissues and fluids from multiple species. (cellsciences.com)
- The disease most commonly associated with the panicum mosaic virus pathogen is St. Augustine Decline Syndrome, which infects species of turf grass and causes chlorotic mottling. (wikipedia.org)
- Specifically, the wasp and ants have an order of magnitude more TNAP families and proteins relative to species that belong to Diptera (mosquitoes and flies). (biomedcentral.com)
- Cauliflower mosaic virus (CaMV) is the type species of the family Caulimoviridae. (wikipedia.org)
- Remarkably, RNase M inhibition was species-specific as Theiler's trojan M* proteins obstructed murine RNase M but not really individual RNase M or RNase M of various other mammals or hens. (acmbcb.org)
- M* activity was discovered to end up being species-specific as it inhibited murine but not really individual RNase M. We verified GSK-3787 IC50 the species-specificity and the immediate connections between GSK-3787 IC50 M* and RNase M within the assembled family members, is normally a organic virus of rodents exhibiting a uncommon occurrence of natural neuro-invasion . (acmbcb.org)
- Because of the change in disease virulence to the main viral pathogen, panicum mosaic virus and satellite panicum mosaic virus are believed to cause synergistic effects to their hosts. (wikipedia.org)
- To determine the relative contribution of each activity to virus virulence, mutant N1 alleles were introduced into a VACV strain lacking N1 and the virulence of these viruses was analysed after intradermal and intranasal inoculation in mice. (biomedsearch.com)
- Unlike the surface antigen, the e-antigen is found in the blood only when there are viruses also present. (abcam.com)
- When the virus goes into "hiding," the e-antigen will no longer be present in the blood. (abcam.com)
- The results of the iTRAQ assays indicated that L antigen family member 3 (LAGE3) protein, essential for tRNA processing, tRNA metabolic processes and ncRNA processing, was downregulated in the PR8+eleu compared with the PR8 group. (spandidos-publications.com)
- Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus. (nih.gov)
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. (rndsystems.com)
- While the VP1u and the VP1/2 common region are not seen in the crystal structures of the viruses, antibody assays confirm that the VP1u is not exposed on the surface of the capsid. (ufl.edu)
- GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. (enquirebio.com)
- QP10912 protein expressed in E. Coli. (enquirebio.com)
- Our preliminary results in overexpression in E. coli and purification of the protein encoded by ORF-1, Rep, are hereby showed. (omicsonline.org)
- Envelope protein E of Zika virus, packaged and ready to ship as plasmid, Adeno or AAV9. (vigenebio.com)
- Envelope protein E: Binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. (vigenebio.com)
- Synthesized as a homodimer with preM which acts as a chaperone for envelope protein E. After cleavage of preM , envelope protein E dissociate from small envelope protein M and homodimerizes. (vigenebio.com)
- [ 12 ] The B2L major envelope protein has lipase activity and immunomodulating properties. (medscape.com)
- However, the envelope protein contained Lys 84, which was specific to strains of genotype 5 viruses from South Korea. (cdc.gov)
- Positive selection was statistically detected only at one single codon coding for amino acids belonging to the hypothesized N-linked glycosylation site of the envelope protein. (wikipedia.org)