DNA-(Apurinic or Apyrimidinic Site) Lyase: A DNA repair enzyme that catalyses the excision of ribose residues at apurinic and apyrimidinic DNA sites that can result from the action of DNA GLYCOSYLASES. The enzyme catalyzes a beta-elimination reaction in which the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. This enzyme was previously listed under EC 188.8.131.52.Apurinic Acid: Hydrolysate of DNA in which purine bases have been removed.Deoxyribonuclease IV (Phage T4-Induced): An enzyme which catalyzes the endonucleolytic cleavage of phosphodiester bonds at purinic or apyrimidinic sites (AP-sites) to produce 5'-Phosphooligonucleotide end products. The enzyme prefers single-stranded DNA (ssDNA) and was formerly classified as EC 184.108.40.206.Carbon-Oxygen Lyases: Enzymes that catalyze the cleavage of a carbon-oxygen bond by means other than hydrolysis or oxidation. EC 4.2.PolynucleotidesN-Glycosyl Hydrolases: A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.Endodeoxyribonucleases: A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.DNA Repair: The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.DNA Glycosylases: A family of DNA repair enzymes that recognize damaged nucleotide bases and remove them by hydrolyzing the N-glycosidic bond that attaches them to the sugar backbone of the DNA molecule. The process called BASE EXCISION REPAIR can be completed by a DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE which excises the remaining RIBOSE sugar from the DNA.Deoxyribonuclease (Pyrimidine Dimer): An enzyme which catalyzes an endonucleolytic cleavage near PYRIMIDINE DIMERS to produce a 5'-phosphate product. The enzyme acts on the damaged DNA strand, from the 5' side of the damaged site.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Uracil-DNA Glycosidase: An enzyme that catalyzes the HYDROLYSIS of the N-glycosidic bond between sugar phosphate backbone and URACIL residue during DNA synthesis.DNA-Formamidopyrimidine Glycosylase: A DNA repair enzyme that is an N-glycosyl hydrolase with specificity for DNA-containing ring-opened N(7)-methylguanine residues.Endonucleases: Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.Deoxyribonucleases: Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Purines: A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.ATP Citrate (pro-S)-Lyase: An enzyme that, in the presence of ATP and COENZYME A, catalyzes the cleavage of citrate to yield acetyl CoA, oxaloacetate, ADP, and ORTHOPHOSPHATE. This reaction represents an important step in fatty acid biosynthesis. This enzyme was formerly listed as EC 220.127.116.11.Lyases: A class of enzymes that catalyze the cleavage of C-C, C-O, and C-N, and other bonds by other means than by hydrolysis or oxidation. (Enzyme Nomenclature, 1992) EC 4.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Kinetics: The rate dynamics in chemical or physical systems.Methyl Methanesulfonate: An alkylating agent in cancer therapy that may also act as a mutagen by interfering with and causing damage to DNA.DNA Polymerase beta: A DNA repair enzyme that catalyzes DNA synthesis during base excision DNA repair. EC 18.104.22.168.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Deoxycytidine Monophosphate: Deoxycytidine (dihydrogen phosphate). A deoxycytosine nucleotide containing one phosphate group esterified to the deoxyribose moiety in the 2'-,3'- or 5- positions.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Polysaccharide-Lyases: A group of carbon-oxygen lyases. These enzymes catalyze the breakage of a carbon-oxygen bond in polysaccharides leading to an unsaturated product and the elimination of an alcohol. EC 4.2.2.Adenylosuccinate Lyase: An enzyme that, in the course of purine ribonucleotide biosynthesis, catalyzes the conversion of 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole to 5'-phosphoribosyl-4-carboxamide-5-aminoimidazole and the conversion of adenylosuccinic acid to AMP. EC 22.214.171.124.Ribosemonophosphates: Ribose substituted in the 1-, 3-, or 5-position by a phosphoric acid moiety.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Exodeoxyribonucleases: A family of enzymes that catalyze the exonucleolytic cleavage of DNA. It includes members of the class EC 3.1.11 that produce 5'-phosphomonoesters as cleavage products.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Exonucleases: Enzymes that catalyze the release of mononucleotides by the hydrolysis of the terminal bond of deoxyribonucleotide or ribonucleotide chains.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Oxo-Acid-Lyases: Enzymes that catalyze the cleavage of a carbon-carbon bond of a 3-hydroxy acid. (Dorland, 28th ed) EC 4.1.3.DeoxyriboseUracilHypertrophy: General increase in bulk of a part or organ due to CELL ENLARGEMENT and accumulation of FLUIDS AND SECRETIONS, not due to tumor formation, nor to an increase in the number of cells (HYPERPLASIA).Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.DNA Ligases: Poly(deoxyribonucleotide):poly(deoxyribonucleotide)ligases. Enzymes that catalyze the joining of preformed deoxyribonucleotides in phosphodiester linkage during genetic processes during repair of a single-stranded break in duplex DNA. The class includes both EC 126.96.36.199 (ATP) and EC 188.8.131.52 (NAD).DioxolanesNucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.DNA Polymerase I: A DNA-dependent DNA polymerase characterized in prokaryotes and may be present in higher organisms. It has both 3'-5' and 5'-3' exonuclease activity, but cannot use native double-stranded DNA as template-primer. It is not inhibited by sulfhydryl reagents and is active in both DNA synthesis and repair. EC 184.108.40.206.Pyrimidine Dimers: Dimers found in DNA chains damaged by ULTRAVIOLET RAYS. They consist of two adjacent PYRIMIDINE NUCLEOTIDES, usually THYMINE nucleotides, in which the pyrimidine residues are covalently joined by a cyclobutane ring. These dimers block DNA REPLICATION.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.GuanineAldehyde-Lyases: Enzymes that catalyze a reverse aldol condensation. A molecule containing a hydroxyl group and a carbonyl group is cleaved at a C-C bond to produce two smaller molecules (ALDEHYDES or KETONES). EC 4.1.2.T-Phages: A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.Borohydrides: A class of inorganic or organic compounds that contain the borohydride (BH4-) anion.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.DNA Adducts: The products of chemical reactions that result in the addition of extraneous chemical groups to DNA.Osmium Tetroxide: (T-4)-Osmium oxide (OsO4). A highly toxic and volatile oxide of osmium used in industry as an oxidizing agent. It is also used as a histological fixative and stain and as a synovectomy agent in arthritic joints. Its vapor can cause eye, skin, and lung damage.Glycolates: Derivatives of ACETIC ACID which contain an hydroxy group attached to the methyl carbon.Phosphorus-Oxygen Lyases: Enzymes that catalyze the cleavage of a phosphorus-oxygen bond by means other than hydrolysis or oxidation. EC 4.6.Electrophoretic Mobility Shift Assay: An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Ultraviolet Rays: That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.Flap Endonucleases: Endonucleases that remove 5' DNA sequences from a DNA structure called a DNA flap. The DNA flap structure occurs in double-stranded DNA containing a single-stranded break where the 5' portion of the downstream strand is too long and overlaps the 3' end of the upstream strand. Flap endonucleases cleave the downstream strand of the overlap flap structure precisely after the first base-paired nucleotide, creating a ligatable nick.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Hydroxylamines: Organic compounds that contain the (-NH2OH) radical.Oligodeoxyribonucleotides: A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Base Pair Mismatch: The presence of an uncomplimentary base in double-stranded DNA caused by spontaneous deamination of cytosine or adenine, mismatching during homologous recombination, or errors in DNA replication. Multiple, sequential base pair mismatches lead to formation of heteroduplex DNA; (NUCLEIC ACID HETERODUPLEXES).Cell Extracts: Preparations of cell constituents or subcellular materials, isolates, or substances.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)DNA-Directed DNA Polymerase: DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Deoxyguanosine: A nucleoside consisting of the base guanine and the sugar deoxyribose.Lucanthone: One of the SCHISTOSOMICIDES, it has been replaced largely by HYCANTHONE and more recently PRAZIQUANTEL. (From Martindale The Extrapharmacopoeia, 30th ed., p46)Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Mutagens: Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
List of MeSH codes (D08)
DNA-(apurinic or apyrimidinic site) lyase MeSH D08.811.074.781 --- muts dna mismatch-binding protein MeSH D08.811.074.812 --- ... DNA-(apurinic or apyrimidinic site) lyase MeSH D08.811.520.241.300 --- hydro-lyases MeSH D08.811.520.241.300.050 --- aconitate ... site-specific dna-methyltransferase (adenine-specific) MeSH D08.811.913.555.500.350.850 --- site-specific dna methyltransferase ... muts dna mismatch-binding protein MeSH D08.811.277.040.025.281 --- muts homolog 2 protein MeSH D08.811.277.040.025.303 --- n- ...
DNA-(apurinic or apyrimidinic site) lyase is an enzyme that in humans is encoded by the APEX1 gene. Apurinic/apyrimidinic (AP) ... "XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions". EMBO J. England ... Mol CD, Hosfield DJ, Tainer JA (2000). "Abasic site recognition by two apurinic/apyrimidinic endonuclease families in DNA base ... sites (also called "abasic sites") occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by ...
DNA-(apurinic or apyrimidinic site) lyase
"A covalent protein-DNA 5'-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1)". Biochem ... In enzymology, DNA-(apurinic or apyrimidinic site) lyase, also referred to as DNA-(apurinic or apyrimidinic site) 5'- ... "Homo sapiens GO:0003906 - DNA-(apurinic or apyrimidinic site) lyase activity". HumanCyc. SRI International. Retrieved March 9, ... Several names for DNA AP lyase include: AP lyase; AP endonuclease class I; endodeoxyribonuclease (apurinic or apyrimidinic); ...
The excision of the bases causes the formation of an apurinic/ apyrimidinic (AP site) gap. These gap sites are mutagenic in ... The presence of disulfide crosslinking gives rise to a complex crystal structure of the MUTY-DNA. The protein structure of the ... namely polymerase and dRP lyase. DNA ligase is used to seal the fragments after dRP excision causes the formation of 5'PO4 that ... The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine ...
... creating an apurinic/apyrimidinic site, commonly referred to as an AP site. This is accomplished by flipping the damaged base ... the protein family, Uracil-DNA glycosylase (UDG) is an enzyme that reverts mutations in DNA. The most common mutation is the ... "Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation". Proc. Natl. Acad. Sci. U.S.A. 103 (31): 11796- ... Uracil-DNA glycosylases are DNA repair enzymes that excise uracil residues from DNA by cleaving the N-glycosydic bond, ...
... apurinic/apyrimidinic) lyase activity introduces nicks in the DNA strand, cleaving the DNA backbone by beta-delta elimination ... phosphodiester bonds of the resulting apurinic/apyrimidinic site (AP lyase activity;EC 220.127.116.11). Fpg has a preference for ... zinc finger straddle the DNA with the long axis of the protein oriented roughly orthogonal to the helical axis of the DNA. ... is a DNA-binding domain found in DNA glycosylase/AP lyase enzymes, which are involved in base excision repair of DNA damaged by ...
FPG IleRS zinc finger
... apurinic/apyrimidinic) lyase activity introduces nicks in the DNA strand, cleaving the DNA backbone by beta-delta elimination ... phosphodiester bonds of the resulting apurinic/apyrimidinic site (AP lyase activity; EC). Fpg has a preference for oxidised ... "Fpg protein of Escherichia coli is a zinc finger protein whose cysteine residues have a structural and/or functional role". J. ... DNA glycosylase/AP lyase enzymes are involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. These ...
found that HGMA2 protein can efficiently cleave DNA containing apurinic/apyrimidinic (AP) sites (is an AP lyase). In addition, ... This gene encodes a protein that belongs to the non-histone chromosomal high-mobility group (HMG) protein family. HMG proteins ... This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the ... Schwanbeck R, Manfioletti G, Wiśniewski JR (2000). "Architecture of high mobility group protein I-C.DNA complex and its ...
Apurinic/apyrimidinic (AP) endonuclease is an enzyme that is involved in the DNA base excision repair pathway (BER). Its main ... Class I AP endonucleases (EC 18.104.22.168) cleave 3' to AP sites by a β-lyase mechanism, leaving an unsaturated aldehyde, termed a ... Mark R. Kelley; Melissa L. Fishel (2007). "The DNA base excision repair protein Ape1/Ref-1 as a Therapeutic and chemopreventive ... Levin, Joshua D; Demple, Bruce (1990). "Analysis of class II (hydrolytic) and class I (beta-lyase) apurinic/apyrimidinic ...
List of EC numbers (EC 4)
DNA-(apurinic or apyrimidinic site) lyase EC 22.214.171.124: transferred entry, now EC 126.96.36.199 EC 188.8.131.52: 2-succinyl-6-hydroxy-2 ... acyl-carrier-protein) dehydratase EC 184.108.40.206: 3-hydroxypalmitoyl-(acyl-carrier-protein) dehydratase EC 220.127.116.11: 5a- ... pectin lyase EC 18.104.22.168: poly(a-L-guluronate) lyase EC 22.214.171.124: xanthan lyase EC 126.96.36.199: exo-(1-4)-α-D-glucan lyase EC 4.2. ... pectate lyase EC 188.8.131.52: poly(b-D-mannuronate) lyase EC 184.108.40.206: chondroitin ABC lyase EC 220.127.116.11: chondroitin AC lyase EC 4.2 ...
Base excision repair
MBD4 protein binds preferentially to fully methylated CpG sites and to the altered DNA bases at those sites. These altered ... "Coordination of Steps in Single-nucleotide Base Excision Repair Mediated by Apurinic/Apyrimidinic Endonuclease 1 and DNA ... Deoxyribose-5-phosphate Lyase Activity in Saccharomyces cerevisiae Trf4 Protein with a Possible Role in Base Excision DNA ... This was the most frequent DNA repair abnormality found among the 8 DNA repair genes tested. NEIL1 was also one of six DNA ...
... of proliferating cell nuclear antigen-dependent repair of apurinic/apyrimidinic sites with purified human proteins". J. Biol. ... only a single nucleotide is added and DNA Polymerase acts as a lyase to excise the AP site. In LP-BER, several bases are ... the DNA ligase becomes associated with the DNA, locates a nick and catalyzes a reaction at the 5' phosphate site of the DNA ... The N-terminus of the protein contains a replication factory-targeting sequence that is used to recruit it to sites of DNA ...
RCSB PDB - 1E9N: A second divalent metal ion in the active site of a new crystal form of human apurinic/apyrimidinic...
Two Divalent Metal Ions in the Active Site of a New Crystal Form of Human Apurinic/Apyrimidinic Endonuclease, Ape1: ... DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE A, B 318 Homo sapiens EC#: 3.1 IUBMB 18.104.22.168 IUBMB Gene Name(s): APEX1 Gene View ... Interactions are determined by geometric criteria as described in K. Stierand, M. Rarey (2010), Drawing the PDB: Protein-ligand ... A second divalent metal ion in the active site of a new crystal form of human apurinic/apyrimidinic endonuclease, Ape1, and its ...
Repository of UOI "Olympias": Expression and subcellular localization of human AP endonuclease 1 (HAP1/Ref-1) protein: a basis...
DNA Repair,*DNA-(Apurinic or Apyrimidinic Site) Lyase,Endothelium, Vascular/metabolism/ultrastructure,Humans, ... AIMS: Human AP endonuclease 1 (HAP1) plays a major role in the repair of apurinic/apyrimidinic (AP) sites in cellular DNA by ... Expression and subcellular localization of human AP endonuclease 1 (HAP1/Ref-1) protein: a basis for its role in human disease ... Unexpectedly for a presumed nuclear protein, the staining pattern in some cell populations was mainly cytoplasmic (e.g. ...
DNA-(apurinic or apyrimidinic site) lyase [Homo sapiens] - Protein - NCBI
DNA-(apurinic or apyrimidinic site) lyase [Homo sapiens] DNA-(apurinic or apyrimidinic site) lyase [Homo sapiens]. gi,18375503, ... DNA-(apurinic or apyrimidinic site) lyase [Homo sapiens]. NCBI Reference Sequence: NP_542379.1 ... DNA complexes with human apurinic/apyrimidinic endonuclease 1: structural insights revealed by pulsed dipolar EPR with ... DNA complexes with human apurinic/apyrimidinic endonuclease 1: structural insights revealed by pulsed dipolar EPR with ...
RCSB PDB - Protein Feature View - DNA-(apurinic or apyrimidinic site) lyase - P27695 (APEX1 HUMAN)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... The C-O-P bond 3 to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3-terminal ... Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions ... Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single ...
APN1 - Apurinic-apyrimidinic endonuclease 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - APN1 gene...
... sites and removes 3-blocking groups present at single strand breaks of damaged DNA. APN1 accounts for > 97% of both apurinic/ ... apyrimidinic (AP) endonuclease and DNA 3-repair diesterase activities. ... DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) ... Pfam protein domain database. More...Pfami. View protein in ... DNA-(Apurinic or apyrimidinic site) lyase. SACPS. 367. APN1 isoform 1 (Fragment). YEASX ...
Protein (Weighted) Links for PubMed (Select 15721984) - Protein - NCBI
8-oxoguanine DNA glycosylase; Includes: RecName: Full=DNA-(apurinic or apyrimidinic site) lyase; Short=AP lyase ... RecName: Full=N-glycosylase/DNA lyase; Includes: RecName: Full= ... PREDICTED: N-glycosylase/DNA lyase isoform X2 [Mus musculus]. ... The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in ... 17 days embryo stomach cDNA, RIKEN full-length enriched library, clone:I920067F05 product:8-oxoguanine DNA-glycosylase 1, full ...
OGG1 - N-glycosylase/DNA lyase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - OGG1 gene & protein
Has a beta-lyase activity that nicks DNA 3 to the lesion. ... DNA repair enzyme that incises DNA at 8-oxoG residues. Excises ... 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. ... apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC. *class III/IV DNA-(apurinic or apyrimidinic site) lyase ... The C-O-P bond 3 to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3-terminal ...
Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1...
... apurinic/apyrimidinic endonuclease, DNA polymerase beta and DNA ligase III. The XRCC1 protein, which is known to bind DNA ... Repair of a uracil-guanine base pair in DNA has been reconstituted with the recombinant human proteins uracil-DNA glycosylase, ... DNA-(Apurinic or Apyrimidinic Site) Lyase * DNA-Binding Proteins / isolation & purification * DNA-Binding Proteins / metabolism ... apurinic/apyrimidinic endonuclease, DNA polymerase beta and DNA ligase III. The XRCC1 protein, which is known to bind DNA ...
Polymorphisms in the DNA repair genes XPD, XRCC1, XRCC3, and APE/ref-1, and the risk of lung cancer among male smokers in...
XRCC3 and apurinic/apyrimidinic endonuclease/redox factor 1 were examined within a randomized clinical trial d … ... Associations between lung cancer risk and common polymorphisms in the DNA repair genes xeroderma pigmentosum complementation ... DNA, Neoplasm / genetics * DNA-(Apurinic or Apyrimidinic Site) Lyase* * DNA-Binding Proteins / genetics* ... XRCC3 and apurinic/apyrimidinic endonuclease/redox factor 1 were examined within a randomized clinical trial designed to ...
Recombinant Human NEIL1 protein (ab123204) | Abcam
Ab123204 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam provides… ... DNA glycosylase/AP lyase Neil1. *DNA-(apurinic or apyrimidinic site) lyase Neil1 ... Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta ... Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. ...
Endonuclease III homolog 2 (Q08214) | InterPro | EMBL-EBI
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity GO:0019104 DNA N-glycosylase activity GO:0051539 4 iron, 4 sulfur ... GO:0006281 DNA repair GO:0006284 base-excision repair GO:0006285 base-excision repair, AP site formation ...
Endonuclease 8-like 1 (Q96FI4) | InterPro | EMBL-EBI
A general role of the DNA glycosylase Nth1 in the abasic sites cleavage step of base excision repair in Schizosaccharomyces...
... apurinic/apyrimidinic, AP) sites that are both cytotoxic and mutagenic, and must be removed efficiently to maintain genetic ... One of the most frequent lesions formed in cellular DNA are abasic ( ... EC 22.214.171.124/Apn2 protein, S pombe; EC 126.96.36.199/DNA-(Apurinic or Apyrimidinic Site) Lyase ... DNA Mutational Analysis. DNA Repair*. DNA-(Apurinic or Apyrimidinic Site) Lyase / genetics, physiology*. Multienzyme Complexes ...
DNA-(apurinic or apyrimidinic site) lyase - Wikipedia
"A covalent protein-DNA 5-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1)". Biochem ... In enzymology, DNA-(apurinic or apyrimidinic site) lyase, also referred to as DNA-(apurinic or apyrimidinic site) 5- ... "Homo sapiens GO:0003906 - DNA-(apurinic or apyrimidinic site) lyase activity". HumanCyc. SRI International. Retrieved March 9, ... Several names for DNA AP lyase include: AP lyase; AP endonuclease class I; endodeoxyribonuclease (apurinic or apyrimidinic); ...
Interactome-wide prediction of short, disordered protein interaction motifs in humans - Molecular BioSystems (RSC Publishing...
FF motif also interact with the protein DNA-(apurinic or apyrimidinic site) lyase (APEX1) (Fig. 2). At least three of the SLiM ... Protein-protein interaction data. Protein-protein interaction (PPI) data was downloaded (5 May 2009) from HPRD,16 BioGRID,40 ... It was estimated that 15-40% of protein-protein interactions may be mediated by SLiMs8 but protein-protein interaction data ... DNA mismatch repair protein MSH3 and A/G-specific adenine DNA glycosylase (MUTYH) (Fig. 2). Given the size and composition of ...
apex2 Summary [species: Xenopus laevis and Xenopus tropicalis] - Xenbase Gene Catalog
Protein RefSeq ,NP_001006804,DNA-(apurinic or apyrimidinic site) lyase 2 [Xenopus tropicalis] ... DNA repair Protein Function :. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester ... apurinic/apyrimidinic endodeoxyribonuclease 2 Synonyms:. ape2 , xth2 , apexl2 ( Add synonyms , Nomenclature history ) Gene ... Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to ...
Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation | PNAS
... apurinic/apyrimidinic) lyase activity then nicks the DNA backbone at the abasic site. Our results suggest that this lyase ... The DNA Repair Protein XRCC1 Functions in the Plant DNA Demethylation Pathway by Stimulating Cytosine Methylation (5-meC) ... APURINIC/APYRIMIDINIC ENDONUCLEASE2 and ZINC FINGER DNA 3-PHOSPHOESTERASE Play Overlapping Roles in the Maintenance of ... Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation. Fernanda Agius, Avnish Kapoor, and Jian-Kang ...
Drosophila Larval Brain Neoplasms Present Tumour-Type Dependent Genome Instability | G3: Genes | Genomes | Genetics
... dOgg1 and Ribosomal protein S3 (RpS3) (Dherin et al. 2000), or "DNA-(apurinic or apyrimidinic site) lyase activity", we ... Pair-end DNA sequencing. DNA samples from tumors and their relative controls were processed in parallel. Genomic DNA of each ... C , A (G , T) transversions are commonly produced by the formation of apurinic (abasic) sites or 8-hydroxy-2′-deoxyguanosine (8 ... Adapter ligation and DNA cluster preparation are performed by Illumina Nextera DNA Sample Preparation KIT (Illumina), and tumor ...
Recombinant Human HMGA1 293 Cell Lysate HMGA1-5480HCL - Creative BioMart
5-deoxyribose-5-phosphate lyase activity; AT DNA binding; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; ... high mobility group protein HMG-I/HMG-Y; HMG-I(Y); high mobility group protein R; high mobility group protein A1; nonhistone ... protein binding; retinoic acid receptor binding; retinoid X receptor binding; sequence-specific DNA binding transcription ... Products > Cancer Proteins > Oncoprotein & suppressor & biomarker > Oncoprotein > other in oncoprotein other in oncoprotein ...
APEX1 antibody | pab50070 | Covalab - Covalab Biotechnology
... apurinic or apyrimidinic site) lyase (APE-1) antibody , P27695 , APEX nuclease (APEN), Apurinic-apyrimidinic endonuclease 1 (AP ... Cross reacts with proteins from mouse, rat and rabbit.. Name. DNA-(apurinic or apyrimidinic site) lyase (APE-1). ... DNA-(apurinic or apyrimidinic site) lyase (APE-1) antibody. APEX nuclease (APEN). Apurinic-apyrimidinic endonuclease 1 (AP ... Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions ...
Structure Summary for 6BOS
List of MeSH codes (D08) - Wikipedia
DNA-(apurinic or apyrimidinic site) lyase MeSH D08.811.074.781 --- muts dna mismatch-binding protein MeSH D08.811.074.812 --- ... DNA-(apurinic or apyrimidinic site) lyase MeSH D08.811.520.241.300 --- hydro-lyases MeSH D08.811.520.241.300.050 --- aconitate ... site-specific dna-methyltransferase (adenine-specific) MeSH D08.811.913.555.500.350.850 --- site-specific dna methyltransferase ... muts dna mismatch-binding protein MeSH D08.811.277.040.025.281 --- muts homolog 2 protein MeSH D08.811.277.040.025.303 --- n- ...
KEGG BRITE: KEGG Orthology (KO) - Rhodococcus opacus PD630
Pd630_LPD04331 DNA-(apurinic or apyrimidinic site) lyase Pd630_LPD02891 DNA-(apurinic or apyrimidinic site) lyase Pd630_ ... DNA repair protein SbcC/Rad50 K03547 sbcD; DNA repair protein SbcD/Mre11 K04485 radA; DNA repair protein RadA/Sms K03629 recF; ... recombination protein RecR K03631 recN; DNA repair protein RecN (Recombination protein N) K10979 ku; DNA end-binding protein Ku ... LPD05019 DNA repair protein recN Pd630_LPD03951 putative DNA repair protein Pd630_LPD04033 Putative DNA ligase-like protein ...
Recombinant Rhesus monkey APEX1 Protein, His-tagged APEX1-353R - Creative BioMart
Recombinant Rhesus monkey APEX1 Protein, His-tagged can be used for research. ... Purified Recombinant Rhesus monkey APEX1 Protein, His-tagged from Creative Biomart. ... APEX1; DNA-(apurinic or apyrimidinic site) lyase;. Gene ID :. 702757. mRNA Refseq :. NM_001258109. ... Recombinant Rhesus monkey APEX1 Protein, His-tagged. Download Datasheet See All APEX1 Products Bring this labeled protein ...
The APEX1 gene and its putative association with human ageing
GO:0003906; DNA-(apurinic or apyrimidinic site) lyase activity. GO:0004519; endonuclease activity. GO:0004520; ... Protein interactions and network. Protein-protein interacting partners in GenAge. TP53, TXN, STAT3, HDAC3, HIF1A, RPA1, UBE2I, ... GO:0003677; DNA binding. GO:0003684; damaged DNA binding. GO:0003691; double-stranded telomeric DNA binding. GO:0003713; ... GO:0006310; DNA recombination. GO:0006351; transcription, DNA-templated. GO:0006355; regulation of transcription, DNA-templated ...
Breast cancer adaptive resistance: HER2 and cancer stem cell repopulation in a heterogeneous tumor society | SpringerLink
Another DNA base damage repair protein up-regulated in radioresistant prostate cancer cells is DNA-(apurinic or apyrimidinic ... site) lyase, which is encoded by the APEX1 gene and normally involved in the repair of pre-mutagenic lesions. It was shown to ... 2007). Accordingly, it is plausible to suggest a link between the activation of DNA damage proteins and NF-κB-HER2-NF-κB ... To support this, our most recent data further suggest that large sets of DNA repair proteins were up-regulated in HER2+ BCSCs ( ...
Recombinant Schizosaccharomyces pombe Uncharacterized protein C17C9.15c(SPAC17C9.15c) - Cusabio
Purchase Recombinant Schizosaccharomyces pombe Uncharacterized protein C17C9.15c(SPAC17C9.15c). It is produced in Yeast. High ... Recombinant Human DNA-(apurinic or apyrimidinic site) lyase(APEX1). Express system: Yeast ... Protein Names. Recommended name: Uncharacterized protein C17C9.15c Storage. The shelf life is related to many factors, storage ... Protein FAQs CUSABIOs Five Expression Systems General Information of Different Tags Production of Recombinant Protein How to ...
NEIL2 Enzyme Human Recombinant | NEH2 Protein | ProSpec
Endonuclease 8-like 2, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, Endonuclease VIII-like ... NEIL2 also has AP (apurinic/apyrimidinic) lyase activity and creates incisions in the DNA strand. NEIL2 cleaves the DNA ... NEIL2 is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL2 has DNA glycosylase ... For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. ...
NEIL2 Antibody (monoclonal) (M01) - Mouse monoclonal antibody raised against a full length recombinant NEIL2. WB, E - Buy Now!...
DNA-(apurinic or apyrimidinic site) lyase Neil2, Endonuclease VIII-like 2, Nei homolog 2, NEH2, Nei-like protein 2, NEIL2. ... The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins ... The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational ... home , Products , Primary Antibodies , NEIL2 Antibody (monoclonal) (M01) NEIL2 Antibody (monoclonal) (M01). Mouse monoclonal ...
Browse our NEIL2 ELISA Kits
Protein level used designations for NEIL2 DNA glycosylase/AP lyase Neil2 , DNA-(apurinic or apyrimidinic site) lyase Neil2 , ... Additionally we are shipping NEIL2 Antibodies (43) and NEIL2 Proteins (8) and many more products for this protein. A total of ... You are viewing an incomplete version of our website. Please click here. to reload the website as full version. ... Results suggest that the decreased DNA repair capacity of the DNA glycosylase NEIL2 R257L variant can induce mutations that ...
NEIL1 Antibody (B-1) | SCBT - Santa Cruz Biotechnology
... apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation. ... The NEIL proteins belong to the FPG familiy of proteins. They act as DNA glycosylases that can recognize and remove damaged ... See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary ... Protein Accession #. OMIM™ Number. Human. NEIL1. 79661. 15q24.2. XM_005254659, XM_006720677, XM_006720678, XM_006720679, XM_ ...
EndonucleaseGlycosylaseGenomeExcisionGlycosylasesNucleasePolymeraseAPEX1DamageSubstratesSynonymsIntroduces nicks in the DNA strandCleavageGenesGuanineNucleotidesPathwayAbasic sitesReactive oxygenGenomeActivityMUTMHumanMammalian cellsMRNAEndonucleasesMetabolismExonucleaseNucleotidePeptideEscherichiaLesionsComplexesLigaseInteractionsPhosphodiester bondStrandResiduesAbstractBase ExcisiChromosomeCatalytic DomainDouble-stranded
- Involvement of two endonuclease III homologs in the base excision repair pathway for the processing of DNA alkylation damage in Saccharomyces cerevisiae. (duke.edu)
- The phosphate backbone adjacent to the resulting apurinic/apyrimidinic (AP) site is then cleaved by an AP endonuclease or glycosylase-associated AP lyase to invoke subsequent BER steps. (duke.edu)
- Here we report that in the absence of the AP endonucleases, deletion of two Escherichia coli endonuclease III homologs, NTG1 and NTG2, partially suppresses MMS-induced killing, which indicates that the AP lyase products are deleterious unless they are further processed by an AP endonuclease. (duke.edu)
- The severe MMS sensitivity seen in AP endonuclease deficient strains can also be rescued by treatment of cells with the AP lyase inhibitor methoxyamine, which suggests that the product of AP lyase action on an AP site is indeed an extremely toxic lesion. (duke.edu)
- We previously reported that yeast cells deficient in the two AP endonucleases (apn1 apn2 double mutant) are extremely sensitive to killing by a model DNA alkylating agent methyl methanesulfonate (MMS) and that this sensitivity can be reduced by deleting the MAG1 3-methyladenine DNA glycosylase gene. (duke.edu)
- Assays with DNA substrates containing base lesions removed by monofunctional DNA glycosylases Udg and MutY showed that Nth1 will also cleave the abasic sites formed by these enzymes and thus act downstream of these enzymes in the BER pathway. (biomedsearch.com)
- At least in vitro , these G/T mismatch repair DNA glycosylases appear to be inefficient demethylases because their 5-methylcytosine DNA glycosylase activity is very low compared to their strong G/T mismatch repair activities ( 12 , 14 , 15 ). (pnas.org)
- Strong genetic evidence in Arabidopsis supports that certain DNA glycosylases function in active DNA demethylation ( 22 , 23 ). (pnas.org)
- Endonuclease 8-like 2 (NEIL2) is a member of a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. (prospecbio.com)
- These glycosylases set off the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break through the associated lyase reaction. (prospecbio.com)
- They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site. (scbt.com)
- Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. (abnova.com)
- The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. (thermofisher.com)
- Recent advances in the structural mechanisms of DNA glycosylases. (jbsdonline.com)
- BER is initiated by the action of DNA N -glycosylases that specifically recognize and excise damaged bases from DNA. (asm.org)
- Using a hidden Markov model analysis based on the crystal structures of several DNA repair proteins, the enzymatic differences between Drosophila and human S3 were suggested by the absence of a conserved glutamine residue in human S3 that usually resides at the cleft of the deduced active site pocket of DNA glycosylases. (iupui.edu)
- Krokan HE, Standal R and Slupphaug G (1997) DNA glycosylases in the base excision repair of DNA. (els.net)
- Using DNA glycosylases, damaged bases can be removed. (wikibooks.org)
- Since many DNA glycosylases have this enzyme activity, the pentenal moiety can then be eliminated by an AP endonuclease to give 3'-OH or by an AP lyase to give 3'-phosphate. (wikibooks.org)
- Enzymes NEIL1 and NEIL2, mammalian DNA glycosylases with β,δ-lyase activity, remove an extensive amount of mutagenic and cytotoxic oxidative pyrimidien lesions and purine-derived formamidopyrimidines. (wikibooks.org)
- DNA glycosylases are key enzymes in the first step of base excision DNA repair, recognizing DNA damage and catalyzing the release of damaged nucleobases. (semanticscholar.org)
- Simple glycosylases, including N -methylpurine DNA glycosylase (MPG), remove the alkylated DNA base, creating an apurinic/apyrimidinic (AP) site but not nicking the DNA backbone. (aacrjournals.org)
- Homo sapiens APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 3, mRNA. (abnova.com)
- GO annotations related to this gene include nuclease activity and double-stranded DNA 3-5 exodeoxyribonuclease activity . (genecards.org)
- DNA-(apurinic or apyrimidinic site) lyase, Apurinic-apyrimidinic endonuclease 1, APEX nuclease, Protein REF-1. (genesilico.pl)
- Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. (string-db.org)
- The system is associated with a large number of Cas accessory proteins among which many contain a CARF (CRISPR-associated Rossmann fold) domain implicated in ligand binding and a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) nuclease domain. (bireme.br)
- Repair of a uracil-guanine base pair in DNA has been reconstituted with the recombinant human proteins uracil-DNA glycosylase, apurinic/apyrimidinic endonuclease, DNA polymerase beta and DNA ligase III. (nih.gov)
- The XRCC1 protein, which is known to bind DNA ligase III, is not absolutely required for the reaction but suppresses strand displacement by DNA polymerase beta, allowing for more efficient ligation after filling of a single nucleotide patch. (nih.gov)
- We show that XRCC1 interacts directly with DNA polymerase beta using far Western blotting, affinity precipitation and yeast two-hybrid analyses. (nih.gov)
- In addition, a complex formed between DNA polymerase beta and a double-stranded oligonucleotide containing an incised abasic site was supershifted by XRCC1 in a gel retardation assay. (nih.gov)
- The region of interaction with DNA polymerase beta is located within residues 84-183 in the N-terminal half of the XRCC1 protein, whereas the C-terminal region of XRCC1 is involved in binding DNA ligase III. (nih.gov)
- Recombinant human DNA Polymerase beta (show POLB ELISA Kits ) and recombinant mpise NEIL2 associated with microtubules in situ and in vivo. (antibodies-online.com)
- During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. (string-db.org)
- Poly (ADP-ribose) polymerase 1 (PARP1) is an ADP-ribosylating enzyme essential for initiating various forms of DNA repair. (mdpi.com)
- Poly (ADP-ribose) polymerase 1 (PARP1) is well known as an ADP-ribosylating enzyme, which becomes activated upon binding to DNA single-strand and double-strand breaks (ssDB and dsDB respectively) [ 1 , 2 , 3 , 4 , 5 , 6 ]. (mdpi.com)
- As an enzyme, PARP1 acts on chromatin remodeling complexes to control DNA accessibility for RNA polymerase [ 18 , 19 , 20 , 21 , 22 ]. (mdpi.com)
- This pathway involves an enzymatic cascade: following removal of the damaged base by a glycosylase, the damage site is treated by an apurinic/apyrimidinic lyase, and then patched by a polymerase. (jbsdonline.com)
- Following appropriate 3′ or 5′ end-trimming steps, DNA polymerase inserts the correct base and DNA ligase seals the nick, thereby repairing the damage ( 4 ). (asm.org)
- In vitro biochemical experiments have demonstrated that the replicative mitochondrial DNA (mtDNA) polymerase gamma, Polg, is a sensitive target for inhibition by metabolically active forms of NRTIs, nucleotide reverse transcriptase inhibitors (NtRTIs). (frontiersin.org)
- However, during normal base excision repair, the SSBs introduced by APE do not progress to DSBs but are instead repaired by DNA polymerase β and ligase. (rupress.org)
- DNA polymerase β has two helix‐hairpin‐helix (HhH) motifs. (els.net)
- The two HhH motifs (white) of DNA polymerase β (red) bind to the sugar-phosphate backbone on both sides of the gapped DNA strand (blue). (els.net)
- Ternary complex structure of DNA polymerase β. (els.net)
- Beard WA and Wilson SH (2000) Structural design of a eukaryotic DNA repair polymerase: DNA polymerase β. (els.net)
- 2000) Mice reconstituted with DNA polymerase β‐deficient fetal liver cells are able to mount a T cell‐dependent immune response and mutate their Ig genes normally. (els.net)
- Gu H, Marth JD, Orban PC, Mossmann H and Rajewsky K (1994) Deletion of a DNA polymerase beta gene segment in T cells using cell type‐specific gene targeting. (els.net)
- Horton JK, Prasad R, Hou E and Wilson SH (2000) Protection against methylation‐induced cytotoxicity by DNA polymerase‐dependent long patch base excision repair. (els.net)
- Ochs K, Sobol RW, Wilson SH and Kaina B (1999) Cells deficient in DNA polymerase are hypersensitive to alkylating agent‐induced apoptosis and chromosomal breakage. (els.net)
- 1993) Yeast open reading frame YCR14C encodes a DNA beta‐polymerase‐like enzyme. (els.net)
- 1996) Specific interaction of DNA polymerase β and DNA ligase I in a multiprotein base excision repair complex from bovine testis. (els.net)
- 1996) Requirement of mammalian DNA polymerase β in base excision repair. (els.net)
- 2000) The lyase activity of the DNA repair protein β‐polymerase protects from DNA‐damage‐induced cytotoxicity. (els.net)
- Sugo N, Aratani Y, Nagashima Y, Kubota Y and Koyama H (2000) Neonatal lethality with abnormal neurogenesis in mice deficient in DNA polymerase. (els.net)
- After the termini of these strands break, they require processing before missing nucleotides can be replaced by DNA polymerase and its strands rejoined by DNA ligases. (wikibooks.org)
- By using DNA polymerase β, 5'-deoxyribose phosphate termini can be converted to 5'-phosphate. (wikibooks.org)
- See 4 reference sequence protein isoforms for the APEX1 gene. (nih.gov)
- The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. (rcsb.org)
- On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS / JUN AP-1 complex after exposure to IR. (rcsb.org)
- Recombinant Rhesus monkey APEX1 full length or partial length protein was expressed. (creativebiomart.net)
- APEX1 repairs oxidative DNA damage. (senescence.info)
- Rabbit polyclonal antibody raised against a full-length human APEX1 protein. (abnova.com)
- APEX1 (NP_001632.1, 1 a.a. ~ 318 a.a) full-length human protein. (abnova.com)
- Human recombinant protein fragment corresponding to amino acids 1-242 of human APEX1 produced in E.coli. (thermofisher.com)
- APEX1 gene amplification and its protein overexpression in osteosarcoma: correlation with recurrence, metastasis, and survival. (uni-muenster.de)
- Base excision repair (BER) is the predominant DNA damage repair pathway for the processing of small base lesions, derived from oxidation and alkylation damages. (nih.gov)
- Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. (rcsb.org)
- A mutant lacking the DNA glycosylase/AP lyase Nth1 is very sensitive to the alkylating agent methyl methanesulfonate (MMS), suggesting a role for Nth1 in base excision repair (BER) of alkylation damage. (biomedsearch.com)
- In E. coli, DNA AP lyase (endonuclease III) helps repair oxidative damage to DNA bases by catalyzing the excision of the damaged pyrimidines and purines from ring saturation or opening from the DNA backbone. (wikipedia.org)
- DNA damage is ubiquitous amongst all forms of life. (wikipedia.org)
- NEIL1 (show NEIL1 ELISA Kits ) and NEIL2 are essential in the recognition of oxidized lesions arising from 8-oxoG and may play important roles in the repair of DNA damage induced by carcinogenic metals. (antibodies-online.com)
- 8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. (thermofisher.com)
- Depressive disorder, including recurrent type (rDD), is accompanied by increased oxidative stress and activation of inflammatory pathways, which may induce DNA damage. (medscimonit.com)
- This thesis is supported by the presence of increased levels of DNA damage in depressed patients. (medscimonit.com)
- Such DNA damage is repaired by the base excision repair (BER) pathway. (medscimonit.com)
- The SNPs of genes involved in the repair of oxidative DNA damage may modulate rDD risk. (medscimonit.com)
- Since this is an exploratory study, the results should to be treated with caution and further work needs to be done to elucidate the exact involvement of DNA damage and repair mechanisms in the development of this disease. (medscimonit.com)
- In particular, the 8‐oxoguanine (GO) lesion is one of the most stable products of oxidative DNA damage ( Dizdaroglu, 1985 ). (embopress.org)
- When left unrepaired, DNA damage can lead to mutagenesis and carcinogenesis. (jbsdonline.com)
- Organisms have evolved to repair DNA damage through several pathways, including the removal and replacement of damaged DNA bases via base excision repair (BER). (jbsdonline.com)
- Wilson, N. & Delaney, S. (2009) Structure-Dependent DNA Damage and Repair in a Trinucleotide Repeat Sequence. (jbsdonline.com)
- Reactive oxygen species (ROS) produced by normal mitochondrial respiration can damage cellular macromolecules, including mitochondrial DNA (mtDNA), and have been implicated in degenerative diseases, cancer, and aging. (asm.org)
- Primary defenses against oxidative damage include protective proteins and small molecules that scavenge ROS or sequester metal ions. (asm.org)
- It has been proposed that mitochondrial mutations, presumably resulting from oxidative mitochondrial DNA (mtDNA) damage, contribute to neurodegenerative disorders, cancer, and the aging process in humans ( 12 , 47 , 55 ). (asm.org)
- Further, the Apn2 endonuclease is required for the viability of smc6 mutants without extrinsic DNA damage, although this is not due to a defect in base excision repair. (sussex.ac.uk)
- Two genes involved in DNA repair and cell cycle control, APEN (apurinic endonuclease) and GADD45 (growth arrest and DNA damage 45) were upregulated. (ox.ac.uk)
- Base excision repair is an important cellular mechanism that protects the integrity of DNA by repairing damage caused by alkylating agents and reactive oxygen species. (els.net)
- The ability of radiation resistant thermophiles to deal with the lethal effects of ionizing radiations like DNA damage, oxidative bursts and protein damage has made them a model system for exobiology and interplanetary transmission of life. (bireme.br)
- When damage is done to cellular DNA, this causes aging, cancer etiology and treatment, and neurological disorders. (wikibooks.org)
- DNA damage comes in different forms like: base modification and base loss and strand breaks. (wikibooks.org)
- These counter mutational and cytotoxic consequences that occur due to DNA damage. (wikibooks.org)
- Oxygen radicals damage chromosonal DNA causing cell death and inducing mutations. (vwr.com)
- Oxidative damage to DNA has been proposed to have a role in cancer and ageing. (takarabio.com)
- Enhancement of temozolomide via methoxyamine and MPG overexpression was analyzed using in vitro assays, including 3-(4-5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) assay, apoptosis via Annexin staining, and Western blotting for H2AX phosphorylation to quantitate DNA damage. (aacrjournals.org)
- Our results show that MPG-overexpressing IGROV-1 and IGROV-1mp53 cells are significantly more sensitive to the clinical chemotherapeutic temozolomide in combination with methoxyamine as assayed by cytotoxicity, apoptosis, and levels of DNA damage than either agent alone. (aacrjournals.org)
- Experiments with different substrates demonstrated that the protein covalently binds to the 5′ DNA product, i.e. the fragment containing an α,β-unsaturated aldehyde. (biochemj.org)
- Much of the seminal work elucidating these repair systems has taken advantage of defined DSB substrates, either constructed in vitro or formed in cells by site-specific nucleases [ 9 ]. (hindawi.com)
- Note on nomenclature: where proteins have multiple synonyms, the long and short names given below are generally those recommended by Uniprot. (olink.com)
- To see all of the synonyms that may apply to a given marker, visit the Uniprot website and search using the unique Uniprot ID number listed for the protein. (olink.com)
Introduces nicks in the DNA strand1
- Also involved in the DNA cleavage step of class switch recombination (CSR). (rcsb.org)
- A general role of the DNA glycosylase Nth1 in the abasic sites cleavage step of base excision repair in Schizosaccharomyces pombe. (biomedsearch.com)
- Analysis of the AP site cleavage in whole cell extracts of wild-type and mutant strains showed that the AP lyase activity of Nth1 represents the major AP site incision activity in vitro. (biomedsearch.com)
- We suggest that the main function of Apn2 in BER is to remove the resulting 3'-blocking termini following AP lyase cleavage by Nth1. (biomedsearch.com)
- Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. (genecards.org)
- Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. (string-db.org)
- Deep sequencing of these fragments identifies protein-bound sites as peaks of protected fragments or troughs of cleavage sites. (elsevier.com)
- Structural basis for the recognition and cleavage of abasic DNA in Neisseria meningitidis. (ox.ac.uk)
- Furthermore, the structural mechanism for AP-site cleavage is incomplete. (ox.ac.uk)
- AIMS: Human AP endonuclease 1 (HAP1) plays a major role in the repair of apurinic/apyrimidinic (AP) sites in cellular DNA by catalysing hydrolytic cleavage of the phosphodiester backbone 5' to the site. (uoi.gr)
- Cleavage of an abasic site. (els.net)
- Products of cleavage by AP endonuclease have 3′‐hydroxyl and 5′‐deoxyribosephosphate termini (lower right) and products of cleavage by AP lyase have 5′‐phosphate and 3′‐deoxyribosephosphate termini (upper right). (els.net)
- Effect of the multifunctional proteins RPA, YB-1, and XPC repair factor on AP site cleavage by DNA glycosylase NEIL1. (semanticscholar.org)
- Associations between lung cancer risk and common polymorphisms in the DNA repair genes xeroderma pigmentosum complementation group D (XPD), X-ray repair cross-complementing group 1 (XRCC1), XRCC3 and apurinic/apyrimidinic endonuclease/redox factor 1 were examined within a randomized clinical trial designed to determine whether alpha-tocopherol, beta-carotene, or both would reduce cancer incidence among male smokers in Finland. (nih.gov)
- Our results suggest that common alterations in single DNA repair genes are not major determinants of lung cancer susceptibility among smokers. (nih.gov)
- We report here the biochemical characterization of ROS1 and the effect of its overexpression on the DNA methylation of target genes. (pnas.org)
- DNA cytosine methylation is important for many epigenetic processes including X chromosome inactivation, genomic imprinting, epigenetic changes during carcinogenesis, and silencing of transposons, of specific genes during development and of certain transgenes ( 1 - 5 ). (pnas.org)
- Therefore, we genotyped nine single-nucleotide polymorphisms (SNPs) in six genes encoding BER proteins. (medscimonit.com)
- Genetic variation in the DNA repair genes might be associated with altered DNA repair capacities (DRC). (nih.gov)
- There is growing evidence describing DNA repair genes polymorphisms are related to increased cancer risk including colorectal cancer (CRC). (cdc.gov)
- Genome-wide distribution of RNA-DNA hybrids identifies RNase H targets in tRNA genes, retrotransposons and mitochondria. (nih.gov)
- Finally, R-loops were detected on actively transcribed protein-coding genes in the wild-type, particularly over the second exon of spliced ribosomal protein genes. (nih.gov)
- Experimental Technique/Method:X-RAY DIFFRACTION Resolution:2.5 Classification:TRANSCRIPTION Release Date:2018-02-28 Deposition Date:2018-02-05 Revision Date: Molecular Weight:55137.11 Macromolecule Type:Protein Residue Count:474 Atom Site Count:3899 DOI:10.2210/pdb6cc0/pdb Abstract: Pseudomonas aeruginosa is an opportunistic pathogen that uses the process of quorum sensing (QS) to coordinate the expression of many virulence genes. (mendeley.com)
- Researchers have also identified that mutations that have lead to changes in PNKP, similar to mutations in other genes that encode other strand break repair proteins, have been connected to a severe autosomal recessive neurological disorder. (wikibooks.org)
- YB-1 is a DNA- and RNA-binding protein that regulates expression of many important genes. (semanticscholar.org)
- Shows robust 3-5 exonuclease activity on 3-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. (genecards.org)
- The altered DNA base, and in some cases a few nucleotides adjacent to the altered base, is removed by excision, and the DNA is repaired by DNA synthesis and ligation. (els.net)
- The deletion of nth1 in the apn2 mutant strain partially relieves the MMS sensitivity of the apn2 single mutant, indicating that the Apn2 and Nth1 act in the same pathway for the repair of abasic sites. (biomedsearch.com)
- Base excision repair (BER) is a highly conserved DNA repair pathway throughout all kingdoms from bacteria to humans. (ox.ac.uk)
- We have analyzed the effect of solute carrier family 2 (facilitated glucose transporter), member 1 polymorphisms on 2-[fluorine-fluoro-2-deoxy-D-glucose-uptake with a combination of polymorphisms of hypoxia-inducible factor 1 alpha, apurinic/apyimidinic endonuclease, and vascular endothelial growth factor A in a hypoxia-related pathway. (cdc.gov)
- Repair of nuclear and mitochondrial oxidative DNA lesions occurs predominantly through the base excision repair (BER) pathway ( 37 ). (asm.org)
- To improve the treatment of women with ovarian cancer, we are investigating the modulation of a prominent DNA-damaging agent, temozolomide, by manipulating the DNA base excision repair (BER) pathway via BER inhibitor, methoxyamine, and overexpression of N -methylpurine DNA glycosylase (MPG). (aacrjournals.org)
- We are focusing on inhibition and manipulation of the DNA base excision repair (BER) pathway in ovarian cancer. (aacrjournals.org)
- Methoxyamine is an alkoxyamine derivative able to block the single nucleotide BER pathway by reacting with the aldehyde group in the acyclic sugar left in the DNA abasic site following the glycosylase-driven removal of a damaged nucleotide ( 6 , 7 ). (aacrjournals.org)
- The crystal structure of the human DNA repair endonuclease HAP1 suggests the recognition of extra-helical deoxyribose at DNA abasic sites. (genesilico.pl)
- APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. (thermofisher.com)
- Free radical-mediated DSBs typically bear unligatable 3′-phosphate or 3′-phosphoglycolate termini and often have oxidized bases and/or abasic sites near the break. (hindawi.com)
- Ku, a core DSB joining protein, can cleave abasic sites near DNA ends. (hindawi.com)
- The initial steps in DSB formation have been elucidated, involving cytosine deamination by activation-induced cytidine deaminase and generation of abasic sites by uracil DNA glycosylase. (rupress.org)
- However, it is not known how abasic sites are converted into single-stranded breaks and, subsequently, DSBs. (rupress.org)
- Apurinic/apyrimidinic endonuclease (APE) efficiently nicks DNA at abasic sites, but it is unknown whether APE participates in CSR. (rupress.org)
- How the abasic sites in S regions are converted to DNA breaks during CSR is unknown. (rupress.org)
- During CSR, DNA abasic sites resulting from UNG activity might be cleaved by APE, but no evidence for involvement of APE in CSR has been reported. (rupress.org)
- The genome exerts its functions through interactions with proteins. (elsevier.com)
- Hence, comprehensive identification of protein-occupied sites by genomic footprinting is critical to an in-depth understanding of genome functions. (elsevier.com)
- DMS added to the culture medium readily enters the cell and methylates its DNA throughout the genome except for the regions bound by proteins, thereby obviating the need for nuclear isolation in genomic footprinting. (elsevier.com)
- Umeyama, T & Ito, T 2018, ' DMS-seq for In Vivo Genome-Wide Mapping of Protein-DNA Interactions and Nucleosome Centers ', Current Protocols in Molecular Biology , vol. 123, no. 1, e60. (elsevier.com)
- Mitochondria are eukaryotic organelles that share bacterial features such as a double-membrane structure and a circular multi-copied genome or mitochondrial DNA (mtDNA). (frontiersin.org)
- With the development of high-throughput sequencing technologies, such as two-hybrid systems and mass spectrometry technology for pairwise protein interactions, large-scale PPI networks of E. coli can be constructed at genome level [ 20 ]. (biomedcentral.com)
- Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. (rcsb.org)
- Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. (rcsb.org)
- Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB . (rcsb.org)
- the interaction stimulates the FOS / JUN AP-1 complex DNA-binding activity in a redox-dependent manner. (rcsb.org)
- Has a beta-lyase activity that nicks DNA 3' to the lesion. (uniprot.org)
- These data indicate that XRCC1, which has no known catalytic activity, might serve as a scaffold protein during base excision-repair. (nih.gov)
- Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. (abcam.com)
- Mn2+ stabilizes the reaction of the lyase activity. (wikipedia.org)
- DNA AP lyase activity is documented to have similar function in both E. Coli and in humans. (wikipedia.org)
- NEIL2 has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double stranded DNA (DNA bubbles). (prospecbio.com)
- This gene encodes a protein shown to have a weak class II AP endonuclease activity. (genecards.org)
- Shows fairly strong 3-phosphodiesterase activity involved in the removal of 3-damaged termini formed in DNA by oxidative agents. (genecards.org)
- The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. (string-db.org)
- moreover, competition with other DNA binding proteins may further reduce the effective adenine glycosylase activity in vivo. (elsevier.com)
- Pope, MA, Chmiel, NH & David, SS 2005, ' Insight into the functional consequences of hMYH variants associated with colorectal cancer: Distinct differences in the adenine glycosylase activity and the response to AP endonucleases of Y150C and G365D murine MYH ', DNA Repair , vol. 4, no. 3, pp. 315-325. (elsevier.com)
- ALKBH1 (AlkB homologue 1) is a mammalian AlkB (2-oxoglutarate-dependent dioxygenase) homologue that possesses AP (abasic or apurinic/apyrimidinic) lyase activity. (biochemj.org)
- In turn, antagonists of QscR with partial agonist activity were found to destabilize and greatly impair QscR dimerization and DNA binding. (mendeley.com)
- In regards to the latter activity, it has been shown that S3 contains vigorous N-glycosylase activity for the removal of 8-oxoguanine residues in DNA that leaves baseless sites in their places. (iupui.edu)
- The human S3 protein, although 80% identical to its Drosophila homolog and shorter by only two amino acids, has only marginal N-glycosylase activity. (iupui.edu)
- Thus, a single amino acid change converted the Drosophila activity into one that is similar to that possessed by the human S3 protein. (iupui.edu)
- On the basis of the three‐dimensional structure and taking account of previous biochemical experiments, we propose a DNA‐binding mode and reaction mechanism for MutM. (embopress.org)
- The mutM ( fpg ) gene encoding the MutM protein is highly conserved across a wide range of aerobic bacteria. (embopress.org)
- DNA complexes with human apurinic/apyrimidinic endonuclease 1: structural insights revealed by pulsed dipolar EPR with orthogonal spin labeling. (nih.gov)
- Combining data from seven different interaction databases, we analysed approximately 6000 protein -centred and 1600 domain-centred human interaction datasets of 3+ unrelated proteins that interact with a common partner. (rsc.org)
- Reacts with human ~37 kDa APE-1 protein. (covalab.com)
- Isolation of cDNA clones encoding a human apurinic/apyrimidinic endonuclease that corrects DNA repair and mutagenesis defects in E. coli xth (exonuclease III) mutants. (genesilico.pl)
- Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q 11.2-12. (genesilico.pl)
- Human apurinic endonuclease gene (APE): structure and genomic mapping (chromosome 14q11.2-12). (genesilico.pl)
- Asparagine 212 is essential for abasic site recognition by the human DNA repair endonuclease HAP1. (genesilico.pl)
- Accompanying the above-mentioned topics, this review examines: (1) mtDNA maintenance in human health and disease, (2) reports of DNA polymerases theta and zeta (Rev3) localizing to mitochondria, and (3) additional drugs with off-target effects on mitochondrial function. (frontiersin.org)
- The total number of different assays for human protein biomarkers currently available via our 13 human panels = 1073 - with more to come! (olink.com)
- In support of this were experiments executed in vivo that showed that human S3 and the Drosophila site-directed glutamine-changed S3 performed poorly when compared with Drosophilawild-type S3 and its ability to protect a bacterial mutant from the harmful effects of DNA-damaging agents. (iupui.edu)
- HDAC_11, Human Recombinant Protein recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. (antibody-antibodies.com)
- Please read carefully the data sheet of the HDAC_11, Human Recombinant Protein. (antibody-antibodies.com)
- HDAC_11, Human Recombinant Protein Human samples 80 % of the research is conducted on human samples. (antibody-antibodies.com)
- GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as HDAC_11, Human Recombinant Protein. (antibody-antibodies.com)
- SUV4_20H2, Active Human Recombinant Protein recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. (antibody-antibodies.com)
- Please read carefully the data sheet of the SUV4_20H2, Active Human Recombinant Protein. (antibody-antibodies.com)
- SUV4_20H2, Active Human Recombinant Protein Human samples 80 % of the research is conducted on human samples. (antibody-antibodies.com)
- One key virulence plasmid contained by the three human-specific pathogens is pCD1/pYv, which encodes a type III secretion system for the delivery of virulence proteins that contribute to internalization into the host cell. (up.ac.za)
- pMT1 encodes a protein, murine toxin, that aids rat-to-human transmission by enhancing survival of the organism in the flea midgut. (up.ac.za)
- Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
- Results suggest a role for NEIL1 (show NEIL1 ELISA Kits ) and 2 as backup deoxyribo-5'-phosphate lyases in mammalian cells. (antibodies-online.com)
- 1998) Different DNA polymerases are involved in short and long patch base excision repair in mammalian cells. (els.net)
- Data on the participation of YB-1 in DNA reparation and transcription, mRNA splicing and translation are systematized. (semanticscholar.org)
- RNA-binding proteins are of vital importance for mRNA functioning. (semanticscholar.org)
- Among these, poly(A)-binding proteins (PABPs) are of special interest due to their participation in virtually all mRNA-dependent events that is caused by their high affinity for A-rich mRNA sequences. (semanticscholar.org)
- Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation. (semanticscholar.org)
- Bacteriophage T4 and Micrococcus luteus UV endonucleases were actually shown not to be under the class of "endonuclease," but rather were β-elimination catalysts for reactions at AP sites at the C3'-O-P bond-thus, classifying them as AP lyases. (wikipedia.org)
- This suppression requires the postreplication repair (PRR) protein Rhp18 and the structure-specific endonucleases Slx1/4 and Mus81/Eme1. (sussex.ac.uk)
- The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
- Local DNA repair then removes the abasic nucleotide and adds back an unmethylated cytosine nucleotide ( 11 ). (pnas.org)
- In certain situations, this is followed by a δ-elimination reaction that ultimately leads to the formation of a single nucleotide gap in DNA bordered by 5′- and 3′-phosphate groups. (iupui.edu)
- Multiple defects in Escherichia coli mutants lacking HU protein. (naver.com)
- Determination of active site residues in Escherichia coli endonuclease VIII. (waw.pl)
- Yeast structural gene (APN1) for the major apurinic endonuclease: homology to Escherichia coli endonuclease IV. (genesilico.pl)
- The Schiff base intermediate formed duringthis reaction between Escherichia coli Fpg and DNA was trapped by reduction with sodium borohydride, and the structure of the resulting covalently cross-linkedcomplex was determined at a 2.1-A resolution. (embl-heidelberg.de)
- AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. (abnova.com)
- Apurinic/apyrimidinic (AP) sites are among the most frequent DNA lesions. (nsu.ru)
- DNA lesions repaired by base excision repair. (els.net)
- Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA. (vwr.com)
- Interactions are determined by geometric criteria as described in K. Stierand, M. Rarey (2010), Drawing the PDB: Protein-ligand complexes in two dimensions, ACS Med. (rcsb.org)
- The interaction is important for DNA repair, as auto-ribosylation is necessary to assemble and activate multiprotein complexes to carry out the process [ 6 , 7 ]. (mdpi.com)
- However, in heterodimeric complexes with its partner basic helix-loop-helix proteins, BETA2 does not appear to be a strong activator of transcription by itself. (asm.org)
- CD81), heat shock proteins, components of the endosomal sorting complexes required for transport (ESCRT), integrins and regulators of intracellular trafficking (e.g. (biomedcentral.com)
- The identification of protein complexes and functional modules of E. coli is essential to reveal the principles of cell organization, process, and function. (biomedcentral.com)
- At present, many studies focus on the detection of E. coli protein complexes based on experimental methods. (biomedcentral.com)
- However, based on the large-scale proteomics data set of E. coli, the simultaneous prediction of protein complexes and functional modules, especially the comparative analysis of them is relatively less. (biomedcentral.com)
- In this study, the Edge Label Propagate Algorithm (ELPA) of the complex biological network was used to predict the protein complexes and functional modules of two high-quality PPI networks of E. coli , respectively. (biomedcentral.com)
- Some novel and significant protein complexes and functional modules were revealed based on ELPA. (biomedcentral.com)
- Moreover, through a comparative analysis of predicted complexes with corresponding functional modules, we found the protein complexes were significantly overlapped with corresponding functional modules, and almost all predicted protein complexes were completely covered by one or more functional modules. (biomedcentral.com)
- Finally, on the same PPI network of E. coli , ELPA was compared with a well-known protein module detection method (MCL) and we found that the performance of ELPA and MCL is comparable in predicting protein complexes. (biomedcentral.com)
- In this paper, a link clustering method was used to predict protein complexes and functional modules in PPI networks of E. coli , and the correlation between them was compared, which could help us to understand the molecular functional units of E. coli better. (biomedcentral.com)
- Experiments and data analysis (algorithmic model) are two effective methods to identify protein complexes and functional modules of E. coli . (biomedcentral.com)
- Revealing protein complexes and functional modules in the E. coli PPI network is an important research topic to understand the essential biological functions of proteins. (biomedcentral.com)
- However, with the release of some high-throughput E. coli PPI datasets in recent years, it has become possible to predict protein complexes and functional modules of E. coli based on complex network models. (biomedcentral.com)
- Often, this was due to the non-independence of the datasets, with many proteins sharing interaction partners or contributing interactions to multiple domain datasets. (rsc.org)
- 8,9 They are known to mediate many important protein - protein interactions in a variety of scenarios, including protein scaffolding ( e.g. 14-3-3), intra- ( e.g. (rsc.org)
- Therefore, DMS-seq provides a unique method for non-targeted profiling of in vivo protein-DNA interactions. (elsevier.com)
- The Histone Core Complex: An Octamer Assembled by Two Sets of Protein-Protein Interactions. (jbsdonline.com)
- Interactions of YB-1 with DNA, mRNAs, and proteins are considered. (semanticscholar.org)
- Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. (rcsb.org)
- DNA strand displacement and excessive gap filling during DNA repair were observed in cell-free extracts of an XRCC1-deficient mutant cell line, in agreement with the results from the reconstituted system. (nih.gov)
- Removes the blocking groups from the 3'-termini of the DNA strand breaks generated by ionizing radiations and bleomycin. (genesilico.pl)
- Most DNA double-strand breaks (DSBs) formed in a natural environment have chemical modifications at or near the ends that preclude direct religation and require removal or other processing so that rejoining can proceed. (hindawi.com)
- DNA strand breaks are often caused by internal and external factors. (wikibooks.org)
- Incompletely repaired methoxyamine-blocked AP sites lead to increases in single-strand breaks and double-strand breaks (DSB) as well as cell death ( 4 , 11 ). (aacrjournals.org)
- The fragment MS Protein Standard represents a new category of using heavy isotope labeled (15N, 13C) Lysine and Arginine residues resulting in more than 99% isotope incorporation, as internal MS standards offering distinct advantages to existing products for relative and absolute quantification. (creative-proteomics.com)
- These findings confirm the classification of ALKBH1 as an AP lyase, identify the primary and a secondary lysine residues involved in the lyase reaction, and demonstrate that the protein forms a covalent adduct with the 5′ DNA product. (biochemj.org)
- During POLB-dependent long patch base excision repair (BER), PARP1 and/or PARP2 is recruited to the BER site along with flap endonuclease FEN1. (nih.gov)
- Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. (abcam.com)
- This protein may play an important role in both nuclear and mitochondrial base excision repair. (genecards.org)
- Mol CD, Hosfield DJ and Tainer JA (2000) Abasic site recognition by two apurinic/apyrimidinic endonuclease families in DNA base excision repair: the 3′ ends justify the means. (els.net)
- A gene on chromosome 6q22.1 that belongs to the regulatory factor X gene family, which encode transcription factors that contain a highly conserved winged-helix DNA-binding domain. (thefreedictionary.com)
- A gene on chromosome 2q11.2 that belongs to the regulatory factor X gene family, the protein product of which is thought to be a transcription factor. (thefreedictionary.com)