Enzymes that catalyze the methylation of arginine residues of proteins to yield N-mono- and N,N-dimethylarginine. This enzyme is found in many organs, primarily brain and spleen.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Enzymes that catalyze the methylation of amino acids after their incorporation into a polypeptide chain. S-Adenosyl-L-methionine acts as the methylating agent. EC 2.1.1.
A 30-kDa COMPLEMENT C1Q-related protein, the most abundant gene product secreted by FAT CELLS of the white ADIPOSE TISSUE. Adiponectin modulates several physiological processes, such as metabolism of GLUCOSE and FATTY ACIDS, and immune responses. Decreased plasma adiponectin levels are associated with INSULIN RESISTANCE; TYPE 2 DIABETES MELLITUS; OBESITY; and ATHEROSCLEROSIS.
Cell surface receptors for ADIPONECTIN, an antidiabetic hormone secreted by ADIPOCYTES. Adiponectin receptors are membrane proteins with multiple cytoplasmic and extracellular regions. They are about 43 kDa and encoded by at least two genes with different affinities for globular and full-length adiponectin.
Differentiated epithelial cells of the INTESTINAL MUCOSA, found in the basal part of the intestinal crypts of Lieberkuhn. Paneth cells secrete GROWTH FACTORS, digestive enzymes such as LYSOZYME and antimicrobial peptides such as cryptdins (ALPHA-DEFENSINS) into the crypt lumen.
Diminished effectiveness of INSULIN in lowering blood sugar levels: requiring the use of 200 units or more of insulin per day to prevent HYPERGLYCEMIA or KETOSIS.
A cellular response to environmental insults that cause disruptions in PROTEIN FOLDING and/or accumulation of defectively folded protein in the ENDOPLASMIC RETICULUM. It consists of a group of regulatory cascades that are triggered as a response to altered levels of calcium and/or the redox state of the endoplasmic reticulum. Persistent activation of the unfolded protein response leads to the induction of APOPTOSIS.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN SUBUNITS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Sites on an antigen that interact with specific antibodies.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
A broad group of eukaryotic six-transmembrane cation channels that are classified by sequence homology because their functional involvement with SENSATION is varied. They have only weak voltage sensitivity and ion selectivity. They are named after a DROSOPHILA mutant that displayed transient receptor potentials in response to light. A 25-amino-acid motif containing a TRP box (EWKFAR) just C-terminal to S6 is found in TRPC, TRPV and TRPM subgroups. ANKYRIN repeats are found in TRPC, TRPV & TRPN subgroups. Some are functionally associated with TYROSINE KINASE or TYPE C PHOSPHOLIPASES.
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
A subgroup of TRP cation channels that contain 3-4 ANKYRIN REPEAT DOMAINS and a conserved C-terminal domain. Members are highly expressed in the CENTRAL NERVOUS SYSTEM. Selectivity for calcium over sodium ranges from 0.5 to 10.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The portion of an interactive computer program that issues messages to and receives commands from a user.
Sequential operating programs and data which instruct the functioning of a digital computer.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.
A Fanconi anemia complementation group protein that undergoes PHOSPHORYLATION by CDC2 PROTEIN KINASE during MITOSIS. It forms a complex with other FANCONI ANEMIA PROTEINS and helps protect CELLS from DNA DAMAGE by genotoxic agents.
Congenital disorder affecting all bone marrow elements, resulting in ANEMIA; LEUKOPENIA; and THROMBOPENIA, and associated with cardiac, renal, and limb malformations as well as dermal pigmentary changes. Spontaneous CHROMOSOME BREAKAGE is a feature of this disease along with predisposition to LEUKEMIA. There are at least 7 complementation groups in Fanconi anemia: FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, and FANCL. (from Online Mendelian Inheritance in Man, http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=227650, August 20, 2004)
A diverse group of proteins whose genetic MUTATIONS have been associated with the chromosomal instability syndrome FANCONI ANEMIA. Many of these proteins play important roles in protecting CELLS against OXIDATIVE STRESS.
A Fanconi anemia complementation group protein that regulates the activities of CYTOCHROME P450 REDUCTASE and GLUTATHIONE S-TRANSFERASE. It is found predominately in the CYTOPLASM, but moves to the CELL NUCLEUS in response to FANCE PROTEIN.
A Fanconi anemia complementation group protein that is the most commonly mutated protein in FANCONI ANEMIA. It undergoes PHOSPHORYLATION by PROTEIN KINASE B and forms a complex with FANCC PROTEIN in the CELL NUCLEUS.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A Fanconi anemia complementation group protein that undergoes mono-ubiquitination by FANCL PROTEIN in response to DNA DAMAGE. Also, in response to IONIZING RADIATION it can undergo PHOSPHORYLATION by ataxia telangiectasia mutated protein. Modified FANCD2 interacts with BRCA2 PROTEIN in a stable complex with CHROMATIN, and it is involved in DNA REPAIR by homologous RECOMBINATION.

Ligand-dependent activation of transcription in vitro by retinoic acid receptor alpha/retinoid X receptor alpha heterodimers that mimics transactivation by retinoids in vivo. (1/6391)

All-trans and 9-cis retinoic acids (RA) signals are transduced by retinoic acid receptor/retinoid X receptor (RAR/RXR) heterodimers that act as functional units controlling the transcription of RA-responsive genes. With the aim of elucidating the underlying molecular mechanisms, we have developed an in vitro transcription system using a chromatin template made up of a minimal promoter and a direct repeat with 5-spacing-based RA response element. RARalpha and RXRalpha were expressed in and purified from baculovirus-infected Sf9 cells, and transcription was carried out by using naked DNA or chromatin templates. Transcription from naked templates was not affected by the presence of RA and/or RAR/RXR heterodimers. In contrast, very little transcription occurred from chromatin templates in the absence of RA or RAR/RXR heterodimers whereas their addition resulted in a dosage-dependent stimulation of transcription that never exceeded that occurring on naked DNA templates. Most importantly, the addition of synthetic agonistic or antagonistic retinoids to the chromatin transcription system mimicked their stimulatory or inhibitory action in vivo, and activation by a RXR-specific retinoid was subordinated to the binding of an agonist ligand to the RAR partner. Moreover, the addition of the p300 coactivator generated a synergistic enhancement of transcription. Thus, the dissection of this transcription system ultimately should lead to the elucidation of the molecular mechanisms by which RAR/RXR heterodimers control transcription in a ligand-dependent manner.  (+info)

Differential plasma membrane targeting of voltage-dependent calcium channel subunits expressed in a polarized epithelial cell line. (2/6391)

1. Voltage-dependent calcium channels (VDCCs) show a highly non-uniform distribution in many cell types, including neurons and other polarized secretory cells. We have examined whether this can be mimicked in a polarized epithelial cell line (Madin-Darby canine kidney), which has been used extensively to study the targeting of proteins. 2. We expressed the VDCC alpha1A, alpha1B or alpha1C subunits either alone or in combination with accessory subunits alpha2-delta and the different beta subunits, and examined their localization immunocytochemically. An alpha1 subunit was only targeted to the plasma membrane if co-expressed with the accessory subunits. 3. The combination alpha1C/alpha2-delta and all beta subunits was always localized predominantly to the basolateral membrane. It has been suggested that this is equivalent to somatodendritic targeting in neurons. 4. In contrast, the alpha1B subunit was expressed at the apical membrane with all the accessory subunit combinations, by 24 h after microinjection. This membrane destination shows some parallels with axonal targeting in neurons. 5. The alpha1A subunit was consistently observed at the apical membrane in the combinations alpha1A/alpha2-delta/beta1b or beta4. In contrast, when co-expressed with alpha2-delta/beta2a, alpha1A was clearly targeted to the basolateral membrane. 6. In conclusion, the VDCC alpha1 subunit appears to be the primary determinant for targeting the VDCC complex, but the beta subunit can modify this destination, particularly for alpha1A.  (+info)

Solution structure of BID, an intracellular amplifier of apoptotic signaling. (3/6391)

We report the solution structure of BID, an intracellular cross-talk agent that can amplify FAS/TNF apoptotic signal through the mitochondria death pathway after Caspase 8 cleavage. BID contains eight alpha helices where two central hydrophobic helices are surrounded by six amphipathic ones. The fold resembles poreforming bacterial toxins and shows similarity to BCL-XL although sequence homology to BCL-XL is limited to the 16-residue BH3 domain. Furthermore, we modeled a complex of BCL-XL and BID by aligning the BID and BAK BH3 motifs in the known BCL-XL-BAK BH3 complex. Additionally, we show that the overall structure of BID is preserved after cleavage by Caspase 8. We propose that BID has both BH3 domain-dependent and -independent modes of action in inducing mitochondrial damage.  (+info)

1,25-Dihydroxyvitamin D3 induces differentiation of a retinoic acid-resistant acute promyelocytic leukemia cell line (UF-1) associated with expression of p21(WAF1/CIP1) and p27(KIP1). (4/6391)

Retinoic acid (RA) resistance is a serious problem for patients with acute promyelocytic leukemia (APL) who are receiving all-trans RA. However, the mechanisms and strategies to overcome RA resistance by APL cells are still unclear. The biologic effects of RA are mediated by two distinct families of transcriptional factors: RA receptors (RARs) and retinoid X receptors (RXRs). RXRs heterodimerize with 1, 25-dihydroxyvitamin D3 [1,25(OH)2D3] receptor (VDR), enabling their efficient transcriptional activation. The cyclin-dependent kinase (cdk) inhibitor p21(WAF1/CIP1) has a vitamin D3-responsive element (VDRE) in its promoter, and 1,25(OH)2D3 enhances the expression of p21(WAF1/CIP1) and induces differentiation of selected myeloid leukemic cell lines. We have recently established a novel APL cell line (UF-1) with features of RA resistance. 1,25(OH)2D3 can induce growth inhibition and G1 arrest of UF-1 cells, resulting in differentiation of these cells toward granulocytes. This 1, 25(OH)2D3-induced G1 arrest is enhanced by all-trans RA. Also, 1, 25(OH)2D3 (10(-10) to 10(-7) mol/L) in combination with RA markedly inhibits cellular proliferation in a dose- and time-dependent manner. Associated with these findings, the levels of p21(WAF1/CIP1) and p27(KIP1) mRNA and protein increased in these cells. Northern blot analysis showed that p21(WAF1/CIP1) and p27(KIP1) mRNA and protein increased in these cells. Northern blot analysis showed that p21(WAF1/CIP1) and p27(KIP1) transcripts were induced after 6 hours' exposure to 1,25(OH)2D3 and then decreased to basal levels over 48 hours. Western blot experiments showed that p21(WAF1/CIP1) protein levels increased and became detectable after 12 hours of 1,25(OH)2D3 treatment and induction of p27(KIP1) protein was much more gradual and sustained in UF-1 cells. Interestingly, the combination of 1, 25(OH)2D3 and RA markedly enhanced the levels of p27(KIP1) transcript and protein as compared with levels induced by 1, 25(OH)2D3 alone. In addition, exogenous p27(KIP1) expression can enhance the level of CD11b antigen in myeloid leukemic cells. In contrast, RA alone can induce G1 arrest of UF-1 cells; however, it did not result in an increase of p21(WAF1/CIP1) and p27(KIP1) transcript and protein expression in RA-resistant cells. Taken together, we conclude that 1,25(OH)2D3 induces increased expression of cdk inhibitors, which mediates a G1 arrest, and this may be associated with differentiation of RA-resistant UF-1 cells toward mature granulocytes.  (+info)

CRE DNA binding proteins bind to the AP-1 target sequence and suppress AP-1 transcriptional activity in mouse keratinocytes. (5/6391)

Previously, we have shown that nuclear extracts from cultured mouse keratinocytes induced to differentiate by increasing the levels of extra-cellular calcium contain Fra-1, Fra-2, Jun B, Jun D and c-Jun proteins that bind to the AP-1 DNA binding sequence. Despite this DNA binding activity, AP-1 reporter activity was suppressed in these cells. Here, we have detected the CREB family proteins CREB and CREMalpha as additional participants in the AP-1 DNA binding complex in differentiating keratinocytes. AP-1 and CRE DNA binding activity correlated with the induction of CREB, CREMalpha and ATF-1 and CREB phosphorylation at ser133 (ser133 phospho-CREB) in the transition from basal to differentiating keratinocytes, but the activity of a CRE reporter remained unchanged. In contrast, the CRE reporter was activated in the presence of the dominant-negative (DN) CREB mutants, KCREB and A-CREB, proteins that dimerize with CREB family members and block their ability to bind to DNA. The increase in CRE reporter activity in the presence of these mutants suggests that CRE-mediated transcriptional activity is suppressed in keratinocytes through protein-protein interactions involving a factor that dimerizes with the CREB leucine zipper. In experiments where the A-CREB mutant was co-transfected with an AP-1 reporter construct, transcriptional activity was also increased indicating that a CREB family member binds AP-1 sites and represses AP-1 transcriptional activity as well. Exogenous expression of the transcriptional repressor CREMalpha down-regulated both CRE and AP-1 reporters in keratinocytes suggesting that this factor may contribute to the suppression of AP-1 transcriptional activity observed in differentiating keratinocytes.  (+info)

Impairment of the proapoptotic activity of Bax by missense mutations found in gastrointestinal cancers. (6/6391)

We have reported previously that codon 169 of the proapoptotic gene BAX is a mutational hot spot in gastrointestinal cancer. Two different mutations were found in this codon, replacing the wild-type threonine by alanine or methionine. To compare the proapoptotic activity of these Bax mutants with wild-type Bax, we established an ecdysone (muristerone A)-inducible system in cultured human embryonal kidney 293 cells. Addition of muristerone A induced a dose-dependent decrease in the viability of cells transfected with wild-type BAX, but this loss of viability was inhibited in cells transfected with BAX mutants. Furthermore, muristerone A induced morphological changes characteristic of apoptosis, including cell shrinkage, rounding, formation of apoptotic bodies, detachment and nuclear condensation and fragmentation, in cells transfected with wild-type BAX. These hallmarks of apoptosis were clearly diminished in cells transfected with BAX mutants. Mutation of threonine 169 did not affect the binding of Bax to Bax, Bcl-2, or Bcl-X(L). These results demonstrate that missense mutations at codon 169 of BAX are functional because they inhibit its apoptotic activity. This is the first report of the functional significance of missense mutations in BAX, or any other proapoptotic member of the Bcl-2 family, in primary human tumors.  (+info)

Auxiliary subunits operate as a molecular switch in determining gating behaviour of the unitary N-type Ca2+ channel current in Xenopus oocytes. (7/6391)

1. We systematically examined the biophysical properties of omega-conotoxin GVIA-sensitive neuronal N-type channels composed of various combinations of the alpha1B, alpha2/delta and beta1b subunits in Xenopus oocytes. 2. Whole-cell recordings demonstrated that coexpression of the beta1b subunit decelerated inactivation, whereas the alpha2/delta accelerated both activation and inactivation, and cancelled the kinetic effects of the beta1b. The alpha2/delta and the beta1b controlled voltage dependence of activation differently: the beta1b significantly shifted the current-voltage relationship towards the hyperpolarizing direction; however, the alpha2/delta shifted the relationship only slightly in the depolarizing direction. The extent of voltage-dependent inactivation was modified solely by the beta1b. 3. Unitary currents measured using a cell-attached patch showed stable patterns of opening that were markedly different among subunit combinations in their kinetic parameters. The alpha2/delta and the beta1b subunits also acted antagonistically in regulating gating patterns of unitary N-type channels. Open time was shortened by the alpha2/delta, while the fraction of long opening was enhanced by the beta1b. The alpha2/delta decreased opening probability (Po), while the beta1b increased Po. alpha1Balpha2/deltabeta1b produced unitary activity with an open time distribution value in between those of alpha1Balpha2/delta and alpha1Bbeta1b. However, both the alpha2/delta and the beta1b subunits reduced the number of null traces. 4. These results suggest that the auxiliary subunits alone and in combination contribute differently in forming gating apparatuses in the N-type channel, raising the possibility that subunit interaction contributes to the generation of functional diversity of N-type channels in native neuronal preparations also.  (+info)

PER and TIM inhibit the DNA binding activity of a Drosophila CLOCK-CYC/dBMAL1 heterodimer without disrupting formation of the heterodimer: a basis for circadian transcription. (8/6391)

The Drosophila CLOCK (dCLOCK) and CYCLE (CYC) (also referred to as dBMAL1) proteins are members of the basic helix-loop-helix PAS (PER-ARNT-SIM) superfamily of transcription factors and are required for high-level expression of the circadian clock genes period (per) and timeless (tim). Several lines of evidence indicate that PER, TIM, or a PER-TIM heterodimer somehow inhibit the transcriptional activity of a putative dCLOCK-CYC complex, generating a negative-feedback loop that is a core element of the Drosophila circadian oscillator. In this report we show that PER and/or TIM inhibits the binding of a dCLOCK-CYC heterodimer to an E-box-containing DNA fragment that is present in the 5' nontranscribed region of per and acts as a circadian enhancer element. Surprisingly, inhibition of this DNA binding activity by PER, TIM, or both is not accompanied by disruption of the association between dCLOCK and CYC. The results suggest that the interaction of PER, TIM, or both with the dCLOCK-CYC heterodimer induces a conformational change or masks protein regions in the heterodimer, leading to a reduction in DNA binding activity. Together with other findings, our results strongly suggest that daily cycles in the association of PER and TIM with the dCLOCK-CYC complex probably contribute to rhythmic expression of per and tim.  (+info)

ES domain oligomerization is necessary and sufficient for c18-induced motility. (A) Schematic of synthesis of ES domain monomer and dimer. Cleavage of an Fc-ES
KCTD21 (potassium channel tetramerization domain containing 21), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
The existence of functional GPCR heteromers represents both a major challenge and an opportunity for neuroscience and drug discovery. The number of GPCRs for which evidence suggests a functional role of heteromerization is rapidly increasing. Given the large number of GPCR genes, their ability to form combinations raises the daunting possibility that there are tens or even hundreds of thousands of unique receptor heteromers in the brain and nervous system. Elucidating the function of each of these potential distinct receptor complexes represents a considerable challenge.. ...
Induce specific interactions between proteins to control signal transduction, proliferation, differentiation, adhesion, transformation, and apoptosis. Previously available from ARIAD as the ARGENT Regulated Homodimerization Kit & AP20187.
Induce specific interactions between proteins to control signal transduction, proliferation, differentiation, adhesion, transformation, and apoptosis. Previously available from ARIAD as the ARGENT Regulated Homodimerization Kit & AP20187.
Dimerization of Silaethylene: Computational Evidence for a Novel Mechanism for the Formation of 1,3-Disilacyclobutane via a 1,2 ...
TY - JOUR. T1 - Maintenance of the DNA-damage checkpoint requires DNA-damage-induced mediator protein oligomerization. AU - Usui, Takehiko AU - Foster, Steven. AU - Petrini, John H. J.. N1 - Open Archive. PY - 2009/1/30. Y1 - 2009/1/30. N2 - Oligomeric assembly of Brca1 C-terminal (BRCT) domain-containing mediator proteins occurs at sites of DNA damage. However, the functional significance and regulation of such assemblies are not well understood. In this study, we defined the molecular mechanism of DNA-damage-induced oligomerization of the S. cerevisiae BRCT protein Rad9. Our data suggest that Rad9s tandem BRCT domain mediates Rad9 oligomerization via its interaction with its own Mec1/Tel1-phosphorylated SQ/TQ cluster domain (SCD). Rad53 activation is unaffected by mutations that impair Rad9 oligomerization, but checkpoint maintenance is lost, indicating that oligomerization is required to sustain checkpoint signaling. Once activated, Rad53 phosphorylates the Rad9 BRCT domain, which attenuates ...
On the cover: Twenty-five TLR4 TIR dimer models in which the BB loop of one TIR domain interacts with the E helix of the other. Toshchakov et al. screened a library of TLR4 TIR-derived decoy peptides to demonstrate that peptides derived from these regions inhibit TLR4 signaling by binding to the TLR4 TIR. Toshchakov, V. Y., H. Szmacinski, L. A. Couture, J. R. Lakowicz, and S. N. Vogel. 2011. Targeting TLR4 signaling by TLR4 Toll/IL-1 receptor domain-derived decoy peptides: Identification of the TLR4 Toll/IL-1 receptor domain dimerization interface. J. Immunol. 186: 4819-4827. ...
Antibodies for proteins involved in protein dimerization activity pathways, according to their Panther/Gene Ontology Classification
Together, the results from this study provide evidence for B1 receptor homo-oligomerization and the importance of this mechanism in the expression of the receptor on the cell surface of HEK293 cells. This mechanism directly translates into the presentation of a constitutive mechanism for cellular signaling through Gq/11-mediated phospholipase Cβ activity. Such homo-oligomers may be a regulatory point in B1 receptor maturation, and their disruption could provide a unique way for silencing signaling through this and other B1 receptor-stimulated pathways during inflammation.. Multiple methods were used to investigate whether B1 receptors form homo-oligomers in HEK293 cells. Although neither of these methods alone is sufficient to prove oligomerization, together they are consistent with such a mechanism. Higher order oligomeric receptor forms were readily apparent on immunoblots of SDS-PAGE gels. Such complexes were also indicated by coimmunoprecipitation of differentially tagged receptors. ...
Thermochemistry of HO2 + HO2 → H2O4: Does HO2 Dimerization Affect Laboratory Studies?: Self-reaction is an important sink for the hydroperoxy radical (HO2) in t
In the field of neurobiology, there is great discussion about which particular species of amyloid-beta oligomers (AβOs) contributes to the pathogenesis of Alzheimers disease (AD), a progressive neurodegenerative disease. Previously, our group has found, using molecular weight cutoff filters (MWCO), that AβOs are primarily 100-300 kilodaltons (kDa). The goal of this project is to verify this finding using size exclusion chromatography (SEC). SEC can be used to determine AβO molecular weight according to the amount of time it takes the AβOs to pass through the SEC column. Overall, the project yielded results showing significant portion of AβOs are either |1300 kDa or around 13 kDa. We are currently investigating further reasons why the MWCO and SEC data yield very different results.
The present work is one of the first characterizations of multimeric intermediates in protein aggregation, and offers direct information about the assembly process. The results of the Ellman assay confirm that the dimer is linked by a disulfide bond, as previously proposed (Manderson et al. 1998; Bauer et al. 2000). Formation of the dimer therefore requires at least unfolding of the proteins main helix (Qi et al. 1997), which protects the free cysteine from the solvent in the native structure. We have previously shown that the Blg oligomers are productive intermediates in aggregation: assembly of aggregates coincides with consumption of oligomers, and purified oligomers at high concentration aggregate very rapidly (Bauer et al. 2000). The molten globule character of the oligomers observed here is perfectly in line with previous studies on other proteins (Booth et al. 1997; Kayed et al. 1999; McParland et al. 2000; Rochet and Lansbury 2000), demonstrating that aggregation is accelerated by ...
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Looking for oligomeric protein? Find out information about oligomeric protein. A protein composed of two or more polypeptide chains Explanation of oligomeric protein
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
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Unscramble dimer, Unscramble letters dimer, Point value for dimer, Word Decoder for dimer, Word generator using the letters dimer, Word Solver dimer, Possible Scrabble words with dimer, Anagram of dimer
Nanoscale organization is crucial to stimulating an immune response. Using self-assembling proteins as multimerization platforms provides a safe and immunogenic system to vaccinate against otherwise weakly immunogenic antigens. Such multimerization platforms are generally based on icosahedral viruse …
ProSpecs Adiponectins include: Adiponectin, Acrp30, AdipoQ, GBP-28, APM-1, ACDC, Trimeric Form, Globular Adiponectin, Human, Mouse, Recombinant
Page contains details about DNA origami-AgNP trimer . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
0027] In an embodiment of the present invention, the hydrocarbon startup stream comprises a stream that is recycled from the reactor outlet or from a fractionation section downstream of the oligomerisation reaction, or a stream that is obtained from an external source. In this embodiment, the hydrocarbon startup stream preferably is less reactive than the hydrocarbon feed stream containing the olefin. We prefer to use as a component of the hydrocarbon startup stream a stream that is less reactive but that is not foreign to the oligomerisation process, and/or which is readily acceptable to the process. Suitable streams are a recycle from the reactor outlet or a recycle from a fractionation section downstream of the oligomerisation reaction. The latter for instance may be a portion of the light byproduct stream in which saturates, which are typically coming in with the feed, are separated from the reactor effluent and are concentrated. This mostly saturate containing light byproduct stream ...
The value derived for Henrys Law constant H relates to the properties of the substance oligomerisation and alkylation reaction products of 2-phenylpropene and phenol [EC no. 700-960-7]. Vapour pressure (VP) and water solubility (WS) were obtained from tests with different samples of the substance (see CSR Chapter 1.3). The molecular weight was determined as average molecular weight of the different commercial products of the substance taking into account the percentage of the components in the products and their respective molecular weights. The 90th percentile of these data was taken as characteristic average molecular weight for the substance (average MW 298 g/mol). The constant H for the substance oligomerisation and reaction product … is calculated using the vapour pressure of 0.044 Pa and the water solubility of 4 mg C/L (= 0.0134 mol/m³), both at 21°C (see Chapter 1.3.). Dimension of H is Pa*m³/mol. H = VP / WS x MW = 0.044 Pa / 4 mg/L x 298 g/mol = 3.278 Pa*m³/mol ...
For the B2RT-STOP-B2RT-2XMYC-vAChT donor construct, attempts were made to optimize the multimerization phenomenon by varying the quantity of substrate DNA and length of time of the Gibson reactions. For optimization of DNA quantity, Xho I/Pst I restriction digests for 20 independent clones resulting from 20-minute Gibson reactions using 200 (Figure S1A), 67 (Figure S1B), or 22 fmol (Figure S1C) of substrate DNA are shown. These restriction digests yielded constant bands of 5087, 1952, and 586 bp as well as variable bands from 642 bp (2XMYC) to 852 bp (7XMYC) in 42bp increments. The flexible linker GGSG was included between each MYC repeat to maximize the antigenic availability of the epitope tag and minimize the chances the tag alters the function of the vAChT protein. For each reaction condition, the MYC repeat number was quantitated as indicated for 200 (Figure S2A), 67 (Figure S2B), and 22 fmol (Figure S2C). Each reaction condition yielded at least one clone containing a 7XMYC repeat ...
An Inter-subunit salt bridge is broken in the active conformation of AP2.Predicted location of the modified worm residues within the inactive (PBD ID: 2VGL) and
Several types of D2R and D1R heteroreceptor complexes were discovered in the indirect and direct pathways of the striatum, respectively. Changes in the functio...
The Vasp Tetramerization Domain is a Right-Handed Coiled Coil Based on a 15-Residue Repeat.. PubMed ID: 15-569-942. From NIHs 3D Print Exchange. ...
Putcha P, Danzer KM, Kranich LR, Scott A, Silinski M, Mabbett S, Hicks CD, Veal JM, Steed PM, Hyman BT, McLean PJ. Brain-permeable small-molecule inhibitors of Hsp90 prevent alpha-synuclein oligomer formation and rescue alpha-synuclein-induced toxicity ...
Whats the best way to set up your grill and smoker? The secret is to have temperature control, and for grills, that almost always means using a 2-zone setup. For smokers, the secret is to get a small hot fire that makes clean blue smoke rather that billowy white smoke.
Soluble oligomeric aggregates of the amyloid-beta peptide (Abeta) have been implicated in the pathogenesis of Alzheimers disease (AD). Although the conformation adopted by Abeta within these aggregates is not known, a beta-hairpin conformation is known to be accessible to monomeric Abeta. Here we show that this beta-hairpin is a building block of toxic Abeta oligomers by engineering a double-cysteine mutant (called Abetacc) in which the beta-hairpin is stabilized by an intramolecular disulfide bond. Abeta(40)cc and Abeta(42)cc both spontaneously form stable oligomeric species with distinct molecular weights and secondary-structure content, but both are unable to convert into amyloid fibrils. Biochemical and biophysical experiments and assays with conformation-specific antibodies used to detect Abeta aggregates in vivo indicate that the wild-type oligomer structure is preserved and stabilized in Abetacc oligomers. Stable oligomers are expected to become highly toxic and, accordingly, we find ...
Oligomerization of membrane proteins has received intense research interest because of their importance in cellular signaling and the large pharmacological and clinical potential this offers. Fluorescence imaging methods are emerging as a valid tool to quantify membrane protein oligomerization at high spatial and temporal resolution. Here, we provide a detailed protocol for an image-based method to determine the number and oligomerization state of fluorescently labeled prototypical G-protein-coupled receptors (GPCRs) on the basis of small out-of-equilibrium fluctuations in fluorescence (i.e., molecular brightness) in single cells. The protocol provides a step-by-step procedure that includes instructions for (i) a flexible labeling strategy for the protein of interest (using fluorescent proteins, small self-labeling tags or bio-orthogonal labeling) and the appropriate controls, (ii) performing temporal and spatial brightness image acquisition on a confocal microscope and (iii) analyzing and ...
Chlamydia infections have an immense impact on public health and are associated with diverse disease manifestations including atherosclerosis, blindness, and sterility. The chlamydial developmental cycle is intrinsically linked with the ability of the organism to cause disease. The mechanisms that regulate the developmental cycle are poorly understood; however, transcription appears to play a governing role. An OmpR/PhoB subfamily response regulator termed ChxR exhibits expression patterns that indicate an important role during the developmental cycle. Previously, ChxR was demonstrated to interact with its own promoter and facilitate the transcriptional activation of the chxR gene. To begin to understand the functional role of ChxR, I identified the DNA sequence recognized by ChxR to identify its gene targets. Primarily using gel mobility shift assays, I determined that ChxR interacts with, and has differential affinity for six binding sites in the chxR promoter region. Using the DNA sequences ...
Six new R3 TACCrCl3 complexes, displaying branching on the second or third carbon of the ligand R group, were prepared for catalytic studies for ethylene oligomerisation and polymerisation. Five of the complexes made were activated with MAO and their reactivity towards ethylene, at ambient pressure and in a pressurised autoclave tested under various conditions. A number of the complexes were found to give good activities and selectivity for ethylene oligomerisation under optimum conditions. Chapter 2 discusses the design and synthesis of new R3 TACCrCl3 complexes displaying branching on the second or third carbon of the ligand R group. The complexes synthesised were characterised by paramagnetic NMR, magnetic moments, mass spectrometry, X-ray crystallography and elemental analysis. Chapter 3 explores the catalysis of an active selective ethylene oligomerisation R3TACCrCb complex which at optimal conditions shows higher activity for oligomerisation (trimerisation to l-hexene and higher oligomers) ...
Desjardins, Sylvie Yolande (1997) N-0 Chelated Nickel(II) Complexes as Catalysts for Ethene Oligomerisation, Polymerisation and Ethene/Carbon Monoxide Co-Polymerisation. Unspecified thesis, University of Tasmania. ...
Hinge region predictor (H-Predictor) predicts putative hinge regions involved in protein oligomerization via the domain-swapping mechanism. Domain swapping is an important mechanism for protein oligomerization, in which a fragment of a protein exchanges with a corresponding fragment of another like protein. The segment of polypeptide chain that links the swapped domain and the main protein is the hinge region. In most experimentally observed domain-swapped oligomers, the swapped domains correspond to one or several secondary structural elements from either the N- or C-termini. Only in some rare instances the swapped domains are positioned in the middle of the protein. The domain-swapped oligomeric structures are, therefore, mainly determined by the location and the properties of the hinge region.. Using a simple contact-based potential for enthalpy and graph theory- based estimation for entropy, H-Predictor quantifies for each residue the propensity as the hinge region. Physically, the ...
The photoreceptor cryptochrome 2 (CRY2) has become a powerful optogenetic tool that allows light-inducible manipulation of various signaling pathways and cellular processes in mammalian cells with high spatiotemporal precision and ease of application. However, it has also been shown that the behavior of CRY2 under blue light is complex, as the photoexcited CRY2 can both undergo homo-oligomerization and heterodimerization by binding to its dimerization partner CIB1. To better understand the light-induced CRY2 activities in mammalian cells, this article systematically characterizes CRY2 homo-oligomerization in different cellular compartments, as well as how CRY2 homo-oligomerization and heterodimerization activities affect each other. Quantitative analysis reveals that membrane-bound CRY2 has drastically enhanced oligomerization activity compared to that of its cytoplasmic form. While CRY2 homo-oligomerization and CRY2-CIB1 heterodimerization could happen concomitantly, the presence of certain ...
The dimerization interface in VraR is essential for induction of the cell wall stress response in Staphylococcus aureus: a potential druggable target. https://t.co/9mtzsf5GDw. ...
Cancer affects millions of people worldwide each year. Although effective treatments have been established for many forms of the disease, these therapies target the tumor suppressor p53, which is not involved in all cancers. It is thus critical to seek novel treatment methods that can affect cancers independent of p53. One group of homologous viruses have been found to code for a set of homologous proteins that selectively induce apoptosis in transformed cells in a p53 independent manner. This group includes the human homolog Torque Teno Virus (TTV). In order to explore TTV VP3s potential as a cancer therapeutic, several experiments concerning localization and multimerization were performed to evaluate and compare the apoptotic pathways of the highly characterized CAV Apoptin and TTV VP3.
The sole role of bradykinin (BK) as an inflammatory mediator is controversial, as recent data also support an anti-inflammatory role for BK in Alzheimers disease (AD). The involvement of two different receptors (B1R and B2R) could be a key to understand this issue. However, although copper and zinc dyshomeo Zinc in the Biosciences
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A dimer is a macromolecule that is composed of two equal components (monomers). These components can be identical (homodimer) or slightly different (heterodimer). ...
The epidermal growth factor receptor (EGFR) is activated by dimerization, but activation also generates higher-order multimers, whose nature and function are poorly understood. We have characterized ligand-induced dimerization and multimerization of EGFR using single-molecule analysis, and show that multimerization can be blocked by mutations in a specific region of Domain IV of the extracellular module. These mutations reduce autophosphorylation of the C-terminal tail of EGFR and attenuate phosphorylation of phosphatidyl inositol 3-kinase, which is recruited by EGFR. The catalytic activity of EGFR is switched on through allosteric activation of one kinase domain by another, and we show that if this is restricted to dimers, then sites in the tail that are proximal to the kinase domain are phosphorylated in only one subunit. We propose a structural model for EGFR multimerization through self-association of ligand-bound dimers, in which the majority of kinase domains are activated cooperatively, ...
The extent to which Rhodopsin family G-protein-coupled receptors (GPCRs) form invariant oligomers is contentious. Recent single-molecule fluorescence imaging studies mostly argue against the existence of constitutive receptor dimers and instead suggest that GPCRs only dimerize transiently, if at all. However, whether or not even transient dimers exist is not always clear due to difficulties in unambiguously distinguishing genuine interactions from chance colocalizations, particularly with respect to short-lived events. Previous single-molecule studies have depended critically on calculations of chance colocalization rates and/or comparison with unfixed control proteins whose diffusional behavior may or may not differ from that of the test receptor. Here, we describe a single-molecule imaging assay that 1) utilizes comparisons with well-characterized control proteins, i.e., the monomer CD86 and the homodimer CD28, and 2) relies on cell fixation to limit artifacts arising from differences in the
A broad aspect of the present disclosure relates to a process for conversion of a hydro- carbonaceous feed comprising at least 20 ppmw, 100 ppmw or 500 ppmw and less than 1000 ppmw, 5000 ppmw or 10000 ppmw halides, to a hydrocarbon produ ...
A RECOMBINANT ECTODOMAIN OF THE RECEPTOR FOR THE STEM-CELL FACTOR (SCF) RETAINS LIGAND-INDUCED RECEPTOR DIMERIZATION AND ANTAGONIZES SCF-STIMULATED CELLULAR-RESPONSES
Godin AG, Costantino S, Lorenzo L-, Swift JL, Sergeev M, Ribeiro-da-Silva A, et al. Revealing protein oligomerization and densities in situ using spatial intensity distribution analysis. Proc Natl Acad Sci USA. 2011;108(17):7010-5. ...
4,4-methylenediphenyldiisocyanate and 2,4-diisocyanatodiphenylmethane and their oligomerisation reaction products with 2,2-oxydiethanol, propane-1,2-diol and butane-1,3-diol ...
4GOD: Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface.
Complete information for KCTD17 gene (Protein Coding), Potassium Channel Tetramerization Domain Containing 17, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for KCTD3 gene (Protein Coding), Potassium Channel Tetramerization Domain Containing 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
FeCl3 oxidation of the racemate of C2 symmetric, inherently chiral, sexithiophene monomer 1 (2,2′-bis(2,2′-bithiophene-5-yl)-3,3′-bithianaphthene) affords a mixture of cyclic oligomers, from the prevailing dimer to traces of the pentamer. The oligomers are constituted by mixtures of stereoisomers which are t
Jury: Prof. P. Gönczy (président) ; Prof. J. Lingner (directeur) ; Prof. V. Dion, Prof. O. Hantschel, Prof. D. Shore (rapporteurs) Public defense: 2014-4-28. ...
Protein Engineering. 5 (3): 197-211. doi:10.1093/protein/5.3.197. PMID 1409539. Loening AM, Fenn TD, Wu AM, Gambhir SS ( ... "Multimerization of Staufen1 in live cells". RNA. 16 (3): 585-97. doi:10.1111/j.1751-1097.1978.tb07621.x. PMC 2822923. PMID ... and is coupled with a closely interacting green fluorescent protein (RrGFP), and a Ca++ activated luciferin binding protein ( ... doi:10.1093/protein/gzl023. PMID 16857694. Anderson JM, Charbonneau H, Cormier MJ (March 1974). "Mechanism of calcium induction ...
"Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins". J. Biol. Chem. 279 (29): 30053-9. ... Amongst known FANC proteins, most evidence points for a direct interaction primarily between FANCA protein and BRCA1. Evidence ... This mechanic is also supported by the protein-protein interactions between BRG1 and both BRCA1 and FANCA, that serve to ... Gordon SM, Buchwald M (2003). "Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid ...
"Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins". J. Biol. Chem. 279 (29): 30053-9. ... Fanconi anemia group G protein is a protein that in humans is encoded by the FANCG gene. FANCG, involved in Fanconi anemia, ... Activated FANCD2 protein co-localizes with BRCA1 (breast cancer susceptibility protein) at ionizing radiation-induced foci and ... Gordon SM, Buchwald M (July 2003). "Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid ...
Cytoskeleton-associated protein 4 is a protein that in humans is encoded by the CKAP4 gene. CKAP4 also historically known as ... Multimerization might in addition limit CLIMP-63's diffusion out of ER-sheets. CLIMP-63 was also shown to bind microtubules ... a surfactant protein A binding protein, on type II pneumocytes". American Journal of Physiology. Lung Cellular and Molecular ... The protein includes a cytosolic segment composed of positively charged amino acid (2-23) which might act as a preponderant ...
The CCD domain has been implicated in multimerization and other protein-protein interactions. The SPRY domain is required for ... Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene. The protein encoded by this ... "Multiple regulatory elements and binding proteins of the 5'-flanking region of the human estrogen-responsive finger protein ( ... TRIM proteins primarily function as ubiquitin ligases that regulate the innate response to infection. TRIM25 localizes to the ...
In fact, when the calcium ion is removed, the protein precipitates out of solution. The protein contains only a single modular ... Myeloperoxidase's characteristics are somewhat different, owing to its multimerization state as well as its alternative heme ... The protein is produced on ribosomes embedded on the surface of the endoplasmic reticulum, since it must be ultimately ... This oxidoreductase protein is encoded by the gene EPX, expressed within these myeloid cells. EPO shares many similarities with ...
The RRE also facilitates multimerization of the Rev proteins, which is required for Rev binding and function. The Rev protein ... The ARM is a highly specific sequence which allows for the multimerization of Rev proteins, prior to RNA binding. A single base ... A novel protein was found to be involved in the translation of gag and env mRNA. The unknown protein functioned by removing ... Rev is a transactivating protein that is essential to the regulation of HIV-1 (and other lentiviral) protein expression. A ...
"Multimerization of the cytoplasmic domain of syndecan-4 is required for its ability to activate protein kinase C". J. Biol. ... The protein is found as a homodimer and is a member of the syndecan proteoglycan family. Syndecan-4 interacts with ... Syndecan-4 is a protein that in humans is encoded by the SDC4 gene. Syndecan-4 is one of the four vertebrate syndecans and has ... Syndecan-4 activates protein kinase C (PKC), an enzyme involved in signal transduction. The variable domain of syndecan-4 could ...
AChR experiences multimerization within the post-synaptic membrane largely due to the signaling molecule Agrin. The axon of the ... Evidence for this can be seen in the up-regulation of genes expressing vesicle proteins shortly after synapse formation as well ... Recently data have emerged showing that the Wnt protein family has roles in the later development of synapse formation and ... Jelks KB, Wylie R, Floyd CL, McAllister AK, Wise P (June 2007). "Estradiol targets synaptic proteins to induce glutamatergic ...
EGF-like domain-containing protein 7 is a protein that in humans is encoded by the EGFL7 gene. Intron 7 of EGFL7 hosts the miR- ... interacts with the gC1q domains and participates in multimerization". FEBS Lett. 484 (2): 164-8. doi:10.1016/S0014-5793(00) ... "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): ... The Egfl7 protein (29 kDa) is composed of several putative domains: a putative cleavable signal peptide at the N-terminal end, ...
They consist of non-magnetic soluble protein complexes generated by multimerization of αCD3- and αCD28 Fab-Streps with a Strep- ... which enables the isolation and purification of sensitive proteins in a native state as wells as intact protein complexes, ... Witte, C. P.; Noël, L. D.; Gielbert, J.; Parker, J. E.; Romeis, T. (2004). "Rapid one-step protein purification from plant ... In combination with the Twin Strep-tag it binds the recombinant protein of interest with an extremely high affinity. This ...
... /PCH2 also functions as a kinetochore protein that interacts with the silencing protein p31-Comet. Meiosis in mammalian ... classes of human papillomavirus type 16 E1 mutants suggest pleiotropic conformational constraints affecting E1 multimerization ... "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research. 11 (10): 1758-65. doi:10.1101/gr.180101. PMC ... The APC is activated by CDC20, a protein that is silenced by the mitotic checkpoint complex (MCC). Of interest in relation to ...
2021). "A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain ...
Cytochrome c oxidase assembly protein COX15 homolog (COX15), also known as heme A synthase, is a protein that in humans is ... COX15 multimerization is important for heme A biosynthesis and/or transfer to maturing COX. COX deficiency is one of the most ... "COX15 - Cytochrome c oxidase assembly protein COX15 homolog - Homo sapiens (Human) - COX15 gene & protein". www.uniprot.org. ... This protein localizes to the inner mitochondrial membrane and involved in heme A biosynthesis. COX15 is also part of a three- ...
Transmembrane protein 59 is a protein that in humans is encoded by the TMEM59 gene. TMEM59 is a membrane bound protein that is ... Transmembrane interactions between TMEM59 and the wnt receptor Frizzled were found to drive receptor multimerization that leads ... however it has been demonstrated that expression of TMEM59 protein inhibits Golgi glycosylation of amyloid precursor protein ( ... "Entrez Gene: TMEM59 transmembrane protein 59". Ullrich S, Münch A, Neumann S, Kremmer E, Tatzelt J, Lichtenthaler SF (July 2010 ...
22 January 2021). "A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor- ... Non-specifically bound proteins are removed by washing the resin with a neutral buffer and the target protein eluted at neutral ... Banerjee A, Howarth M (June 2018). "Nanoteamwork: covalent protein assembly beyond duets towards protein ensembles and ... but since the protein has sequence similarity to the parent protein where SpyTag/SpyCatcher is derived from, this Tag/Catcher ...
... mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein". Mol. Cell. Biol. 23 (23): 8751- ... Ran-binding protein 3 is a protein that in humans is encoded by the RANBP3 gene. This gene encodes a protein with a RanBD1 ... 2001). "Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export". J. Cell Biol. 153 (7): 1391-402. doi: ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...
... is a serine/threonine kinase composed of a kinase domain and a coiled-coil domain involved in the multimerization. The crystal ... Myotonin-protein kinase (MT-PK) also known as myotonic dystrophy protein kinase (MDPK) or dystrophia myotonica protein kinase ( ... For example, myotonic dystrophy protein kinase has been shown to turn off (inhibit) part of a muscle protein called myosin ... a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase". The Journal ...
"TRIP Database". a manually curated database of protein-protein interactions for mammalian TRP channels.. ... which is involved with gating modulation and channel multimerization. Other C-terminal modifications such as alpha-kinase ... The identity of the mutated protein was unknown until it was cloned by Craig Montell, a post-doctoral researcher in Gerald ... PKD1-like genes encode much larger proteins with 11 transmembrane segments, which do not have all the features of other TRP ...
... or postsynaptic density protein 93 (PSD-93) is a protein that in humans is encoded by the DLG2 gene. Chapsyn-110/PSD-93 a ... Hsueh YP, Kim E, Sheng M (1997). "Disulfide-linked head-to-head multimerization in the mechanism of ion channel clustering by ... "Synaptic targeting of the postsynaptic density protein PSD-95 mediated by lipid and protein motifs". Neuron. 22 (3): 497-509. ... The protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric ...
It has been suggested that the EMI domain could be a protein-protein interaction module, as the EMI domain of EMILIN-1 was ... interacts with the gC1q domains and participates in multimerization". FEBS Lett. 484 (2): 164-8. doi:10.1016/S0014-5793(00) ... Vertebrate Emu proteins, which could interact with several different extracellular matrix components and serve to connect and ... In molecular biology, the EMI domain, first named after its presence in proteins of the EMILIN family, is a small cysteine-rich ...
Activin, inhibin and a number of other structurally related proteins such as anti-Müllerian hormone, bone morphogenetic protein ... Smad3 then translocates to the nucleus and interacts with SMAD4 through multimerization, resulting in their modulation as ... and growth differentiation factor belong to the TGF-β protein superfamily. The activin and inhibin protein complexes are both ... In addition, both complexes are derived from the same family of related genes and proteins but differ in their subunit ...
A search for protein(s) responsible for macrodomain formation led to identification of Macrodomain Ter protein (MatP). MatP ... July 2016). "HU multimerization shift controls nucleoid compaction". Science Advances. 2 (7): e1600650. Bibcode:2016SciA.... ... Hwang DS, Kornberg A (November 1992). "Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or ... Besides these examples, the chromosome is also specifically anchored to the cell membrane through protein-protein interaction ...
ParA proteins from different plasmids and bacterial species show 25 to 30% of sequence identity to the protein ParA of the ... Surtees, Jennifer A.; Funnell, Barbara E. (1999). "P1 ParB Domain Structure Includes Two Independent Multimerization Domains". ... StbA protein is a DNA-binding protein (identical to ParM) and is strictly required for the stability and intracellular ... A centromere-like DNA site Centromere binding proteins (CBP) The motor protein The centromere-like DNA site is required in cis ...
Proteins: consisting of gag proteins, protease (PR), pol proteins, and env proteins. Group-specific antigen (gag) proteins are ... The SFV capsid is assembled in the cytoplasm as a result of multimerization of Gag molecules, but unlike other related viruses ... Pol proteins are responsible for synthesis of viral DNA and integration into host DNA after infection. Env proteins are ... "bet - Protein Bet - Simian foamy virus (isolate chimpanzee) (SFVcpz) - bet gene & protein". www.uniprot.org. Retrieved 2017-11- ...
Although the protein created localizes within the nucleus and is barely detectable in axons, studies suggest that its ... two sterile alpha motifs responsible for multimerization, and a C-terminus Toll/Interleukin-1 receptor that possesses enzymatic ... The SARM1 protein has four domains, a mitochondrial localization signal, an auto-inhibitory N-terminus region consisting of ... The protective effect of the WldS protein has been shown to be due to the NMNAT1 region's NAD+ synthesizing active site. ...
The PUMA protein is part of the BH3-only subgroup of Bcl-2 family proteins. This group of proteins only share sequence ... another hypothesis is that PUMA directly activates Bax and/or Bak and through Bax multimerization triggers mitochondrial ... member of the Bcl-2 protein family. In humans, the Bcl-2-binding component 3 protein is encoded by the BBC3 gene.The expression ... PUMA protein is degraded in a proteasome dependent manner and its degradation is regulated by phosphorylation at a conserved ...
... or multimerization (e.g., streptavidin or leucine zippers). Fusion proteins can also be manufactured with toxins or antibodies ... Examples include: Gag-onc fusion protein Bcr-abl fusion protein Tpr-met fusion protein Antibodies are fusion proteins produced ... Fusion proteins or chimeric (kī-ˈmir-ik) proteins (literally, made of parts from different sources) are proteins created ... Protein linkers aid fusion protein design by providing appropriate spacing between domains, supporting correct protein folding ...
... protein plays a central role in the Wallerian degeneration pathway. The gene was first identified in a Drosophila ... two sterile alpha motifs responsible for multimerization, and a C-terminus Toll/Interleukin-1 receptor that possesses enzymatic ... The SARM1 protein has four domains, a mitochondrial localization signal, an auto-inhibitory N-terminus region consisting of ... Bayless AM, Nishimura MT (2020). "Enzymatic Functions for Toll/Interleukin-1 Receptor Domain Proteins in the Plant Immune ...
Clustered Pcdhs proteins are detected throughout the neuronal soma, dendrites and axons and are observed in synapses and growth ... If all three classes of Pcdhs could engage in multimerization of stochastically expressed Pcdhs isoforms, then neurons could ... As for cytoplasmic domain, all the three classes of clustered Pcdhs proteins are dissimilar, although they are strictly ... Schalm SS, Ballif BA, Buchanan SM, Phillips GR, Maniatis T (August 2010). "Phosphorylation of protocadherin proteins by the ...
... guanylate-binding proteins (GBP), immunity-related GTPase proteins (IRGs), and very large inducible GTPase proteins (VLIG). ... showing cooperative enzymatic activity and GTP-dependent multimerization". J Biol Chem. 278 (31): 29336-43. doi:10.1074/jbc. ... LC3 is a microtubule associated soluble protein found in mammalian tissues. Cytoplasmic proteins and organelles are engulfed by ... Interferon (IFN)-inducible GTPases encompass four families of proteins including myxovirus resistant proteins (Mx), ...
... bone morphogenetic protein, and growth differentiation factor belong to the TGF-β protein superfamily.[9] ... Smad3 then translocates to the nucleus and interacts with SMAD4 through multimerization, resulting in their modulation as ... The activin and inhibin protein complexes are both dimeric in structure, and, in each complex, the two monomers are linked to ... Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Identified ...
protein homodimerization activity. • GO:0001948 protein binding. • identical protein binding. • extracellular matrix structural ... "Up- and down-regulation of adiponectin expression and multimerization: mechanisms and therapeutic implication". Biochimie. 94 ... protein localization to plasma membrane. • negative regulation of hormone secretion. • protein homooligomerization. • response ... negative regulation of protein autophosphorylation. • negative regulation of intracellular protein transport. • membrane ...
It arises from a deficiency in the quality or quantity of von Willebrand factor (VWF), a multimeric protein that is required ... by its decreased ability to bind to GPIb receptor on the platelet membrane and normal capability at multimerization. The VWF ... The VWF is qualitatively normal and genetic testing of the von Willebrand gene and VWF protein reveals no mutational alteration ... so that virtually no VWF protein is produced. The clinical picture in pigs is most similar to that in humans with VWD type 3. ...
Gross S, Blanchetot C, Schepens J, Albet S, Lammers R, den Hertog J, Hendriks W (2002). "Multimerization of the protein- ... The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Protein tyrosine phosphatases ... Disruption of cadherin proteins, by genetic alteration or by changes to the structure or function of the protein, has been ... Rosdahl JA, Mourton TL, Brady-Kalnay SM (2002). "Protein kinase C delta (PKCdelta) is required for protein tyrosine phosphatase ...
C. Wang, R.J. Stewart, J. Kopeček, Hybrid Hydrogels Assembled from Synthetic Polymers and Coiled-Coil Protein Domains. Nature ... Broadening and Enhancing Functions of Antibodies by Self-Assembling Multimerization at Cell Surface. ACS Nano 13, 11422-11432 ( ...
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling ... Tyrosine phosphorylation of Y912 results in increased multimerization of PTPrho, likely in cis, with other PTPrho molecules. ... This suggests that these proteins are PTPrho substrates. PTPrho also dephosphorylates BCR protein. The ability of PTPrho to ... which encode distinct proteins, have been reported. The first isoform encodes the larger version of the protein. The second ...
The headgroups are connected via flexible linkers to a coiled-coil multimerization domain, which in turn is connected to five ... MHC tetramers consist of four MHC molecules, a tetramerization agent and a fluorescently labeled protein (usually streptavidin ...
PIC is a multimeric protein structure inside the host cell, composed of both viral and host proteins. Integrase is a part of ... The multimerization requires zinc atom that stabilizes the fold. 2. The catalytic core domain (CCD), which encompasses amino ... Inhibitors of this protein are already being developed and patented. They are likely to be highly target specific and less ... If it were possible to exclude certain proteins from the PIC it would block the ability of the virus to integrate into the host ...
The AD hallmark proteins tau in NFTs or GVBs and TAR DNA-binding protein of 43 kDa (TDP-43) in GVBs colocalize with CK1δ. In ... levels in adipose tissue of morbid obese patients and site-specific phosphorylation mediated by CK1 influences multimerization ... So far, C-terminal phosphorylation of CK1δ by upstream kinases has been confirmed for protein kinase A (PKA), protein kinase B ... Locasale JW, Shaw AS, Chakraborty AK (August 2007). "Scaffold proteins confer diverse regulatory properties to protein kinase ...
"Protein Multimerization" by people in Harvard Catalyst Profiles by year, and whether "Protein Multimerization" was a major or ... The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN ... "Protein Multimerization" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Protein Multimerization" by people in Profiles. ...
ATP binding to the sigma 54-dependent activator XylR triggers a protein multimerization cycle catalyzed by UAS DNA.. Pérez- ... ATP hydrolysis by XylR, the cognate regulator of the system, is preceded by the multimerization of XylR at the enhancer, which ... is itself triggered by the sole allosteric effect of ATP binding to the protein. Since ADP is unable to support multimerization ... This notion is supported further by the properties of mutant proteins that seem to be frozen, in either the nonmultimerized or ...
... arginines in proteins. We investigated the expressions of PRMT1 and PRMT5 in relation to their catalytic activities in rat ... Protein-arginine methylation is a posttranslational modification which yields monomethyl and dimethyl (asymmetric or symmetric ... Multimerization of expressed protein-arginine methyltransferases during the growth and differentiation of rat liver Biochim ... In fetal rat liver, the amount of expressed 42 kDa PRMT1 protein and the enzyme activity to methylate hnRNPA1 protein were 2- ...
Protein Lysates, Immunoprecipitation, and Western Blot. Protein lysate, cytosol and nuclear protein extraction, ... protein disulfide isomerase family A, member 6, ER protein 44, and disulfide bond oxidoreductase A-like protein. Thus, we ... A disulfide-bond A oxidoreductase-like protein (DsbA-L) regulates adiponectin multimerization. Proc Natl Acad Sci USA 2008;105: ... Adipocyte Spliced Form of X-Box-Binding Protein 1 Promotes Adiponectin Multimerization and Systemic Glucose Homeostasis. ...
For enhancing the sensitivity of protein detection this epitope multimerization strategy is an alternative to the spaghetti ... Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using ... Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using ... Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using ...
Recombinant Proteins / metabolism * Sialic Acids / metabolism* * Sialyltransferases / metabolism* Substances * ADIPOQ protein, ... Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in ...
Multimerization of the kinase domain may enable the assembly of complexes of downstream proteins and could be important for Eph ... Proteins are essential for all known forms of life and in many lethal diseases protein failure is the cause of the disease. To ... to detect millisecond dynamics at Cα positions in proteins, using the CPMG dispersion relaxation experiment at lower protein ... To better understand proteins, not only do we need to study them, but also develop the methods we are using. This thesis ...
Biochemical properties of bacterial reverse transcriptase-related (rvt) gene products: multimerization, protein priming, and ... The C-terminal domain may be capable of protein priming, which is abolished by site-directed mutagenesis of the catalytic ... Furthermore, we investigated the highly conserved N- and C-terminal domains, which distinguish RVT proteins from other RTs. We ... To investigate biochemical properties of bacterial RVTs, we conducted in vitro studies of recombinant HaRVT protein from the ...
Biochemical properties of bacterial reverse transcriptase-related (rvt) gene products : multimerization, protein priming, and ... Oligomer; Reverse transcription; RNA-dependent DNA polymerase; Coiled-coil; Multimerization Abstract. Cellular reverse ... The C-terminal domain may be capable of protein priming, which is abolished by site-directed mutagenesis of the catalytic ... Further, we investigated the highly conserved N- and C-terminal domains, which distinguish RVT proteins from other RTs. We show ...
Protein (lb); protein (nb); Protéin (su); Protein (hif); 朊 (lzh); بروتين (ar); Protein (br); ပရိုတိန်း (my); 蛋白質 (yue); Белок ( ... प्रोटिन (dty); Prótín (is); Protein (ms); protein (tr); لحمیات (ur); Bielkovina (sk); білок (uk); 蛋白质 (zh-cn); Protein (gsw); ... protein (sco); Уураг (mn); protein (nn); ಪ್ರೋಟೀನ್ (kn); پرۆتین (ckb); protein (en); fehérje (hu); પ્રોટિન (gu); प्रोटिन (new); ... protein (hr); протеин, белки, протеины (ru); протеин (tt-cyrl); protein, Bílkoviny ve výživě člověka (cs); Protein (nutrien) ( ...
Protein Multimerization; and Influenza A Virus, H1N1 Subtype, while the other 97 terms were introduced before 1965 with a ...
... molecule is characterized by multimerization. To establish the formation of Tg multimers, the partially unfolded/reduced Tg or ... Multimerization of Bovine Thyroglobulin, Partially Unfolded or Partially Unfolded/Reduced; Involvement of Protein Disulfide ... Gilbert, H. F., Protein Disulfide Isomerase and Assisted Protein Folding. J. BioI. Chem., 272, 29399-29402 (1997) ... Freedman, R. B., Hirst, T. R., and Tuite, M. F., Protein disulphide isomerase: building bridges in protein folding. Trends ...
Stochastic protein multimerization, activity, and fitness.. Hagner K, Setayeshgar S, Lynch M. ... Evolution of protein interfaces in multimers and fibrils.. Zabel WJ, Hagner KP, Livesey BJ, Marsh JA, Setayeshgar S, Lynch M, ...
Conformational changes of the N-terminal part of Mason-Pfizer monkey virus p12 protein during multimerization. *Knejzlík Z ... Conformational changes of the N-terminal part of Mason-Pfizer monkey virus p12 protein during multimerization. Virology, 393(1 ... fibers is linked to a conformational transition from unfolded or another non-periodical state to α-helix during multimerization ...
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces ... A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike prot ...
Protein Interaction Domains and Motifs , Protein Multimerization , Swine ... A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces ... In this study, we describe a protein nanoparticle vaccine against SARS-CoV-2. The vaccine is based on the display of ... A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike prot ...
The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that ... Oncogene Proteins, Viral / genetics * Oncogene Proteins, Viral / metabolism* * Protein Multimerization Substances * Eco-Q ... The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that ...
In a protein-centric-model, the neck would function as the specificity determinant of the MPER multimerization motif, directing ... a distance only stably observed during direct protein-protein interactions [49]. ARV and NBV p10 proteins were C-terminally ... where f is the proportion of functional p10 proteins, n is the number of p10 proteins in a cluster, and s is the number of ... Cholesterol-dependent, higher-order multimerization is evident with other proteins, such as HIV-1 Gag, where initial membrane ...
MultimerizationUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. Q73448. T1RLW6. C4N7I9. A8VH41. T1RMG2. F1K922. ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. Q73448. P12504. B2CPZ0. I6QLX3. I3VA03. I6R0C5. ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ...
MultimerizationUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. Q73422. Q73427. Q73424. Q73423. Q73421. Q73419. ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. Q73422. P20877. D3XNG9. G8I1N8. I6QQB6. ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ...
Protein Engineering. 5 (3): 197-211. doi:10.1093/protein/5.3.197. PMID 1409539. Loening AM, Fenn TD, Wu AM, Gambhir SS ( ... "Multimerization of Staufen1 in live cells". RNA. 16 (3): 585-97. doi:10.1111/j.1751-1097.1978.tb07621.x. PMC 2822923. PMID ... and is coupled with a closely interacting green fluorescent protein (RrGFP), and a Ca++ activated luciferin binding protein ( ... doi:10.1093/protein/gzl023. PMID 16857694. Anderson JM, Charbonneau H, Cormier MJ (March 1974). "Mechanism of calcium induction ...
Human TRPV4 Channel Splice Variants Revealed a Key Role of Ankyrin Domains in Multimerization and Trafficking ... transient receptor potential protein; TRPL, TRP-like protein; LIC, light-induced current. ... INAF, a protein required for transient receptor potential Ca2+ channel function. C. Li, C. Geng, H.-T. Leung, Y. S. Hong, L. L ... Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry. B. Gillo, I. ...
Analysis of protein-DNA interactions using FAMS. (A) Analysis of interactions using FAMS. By using a labeled macromolecule (MA ... Template-directed multimerization (or aggregation) of biomolecules. ... Application of FAMS to Protein-DNA Interactions. We studied the sequence-specific interaction of E. coli CAP (16) with DNA as a ... FAMS Can Detect Dimerization. Oligomerization is a common modulator of protein function. Because S is sensitive to the ratio of ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. ... This protein in other organisms (by gene name): O14980 - Homo sapiens 6 * P30822 - Saccharomyces cerevisiae (strain ATCC 204508 ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ...
Protein Binding. Protein Kinases / chemistry, genetics, metabolism. Protein Multimerization. Protein Structure, Tertiary. ... 0/Bacterial Proteins; 9005-49-6/Heparin; 9050-30-0/Heparitin Sulfate; EC 2.7.-/Protein Kinases ... Bacterial Proteins / chemistry, genetics, metabolism*. Bacteroides / genetics, metabolism*. Binding Sites / genetics. Cell ... 22539977 - Role of dlg5/lp-dlg, a membrane-associated guanylate kinase family protein, in epitheli.... 22387217 - Indirubin ...
Protein Multimerization. The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from ... Photosystem Ii Protein Complex. A large multisubunit protein complex found in the THYLAKOID MEMBRANE. It uses light energy ... protein-protein interactions. In higher plants thylakoids, the interactions lead to a lateral asymmetry in localization of ... Ubiquitin-protein Ligase Complexes. Complexes of enzymes that catalyze the covalent attachment of UBIQUITIN to other proteins ...
Protein Multimerization. Protein Structure, Quaternary. Protein Structure, Tertiary. Protein Subunits. Recombinant Fusion ... 0/Intracellular Signaling Peptides and Proteins; 0/NEDD8 protein, human; 0/Protein Subunits; 0/Recombinant Fusion Proteins; 0/ ... Intracellular Signaling Peptides and Proteins / chemistry*, genetics, metabolism*. Models, Molecular. Molecular Dynamics ... Ubiquitins; 74-79-3/Arginine; 7440-66-6/Zinc; EC 3.4.-/Peptide Hydrolases; EC 3.4.-.-/COPS5 protein, human ...
Multimerization of TrxL2 led to the aggregation of a substantial fraction of the multimeric proteins. This aggregation effect ... Protein concentration was determined with Coomassie blue G250 (Bio-Rad protein assay dye reagent) using bovine serum albumin as ... As shown by the representative results of the 3×-TrxL2 and 4×-TrxL2 proteins presented in Fig. 6, most of the untreated protein ... The oligomerization domain of C4-binding protein (C4bp) acts as an adjuvant, and the fusion protein comprised of the 19- ...
"Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins". J. Biol. Chem. 279 (29): 30053-9. ... Fanconi anemia group G protein is a protein that in humans is encoded by the FANCG gene. FANCG, involved in Fanconi anemia, ... Activated FANCD2 protein co-localizes with BRCA1 (breast cancer susceptibility protein) at ionizing radiation-induced foci and ... Gordon SM, Buchwald M (July 2003). "Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid ...
V. Liposomes, protein purification, and antibody digestion - Large molecule expertise at IPDC includes conjugation of proteins ... V. Peptide optimization and multimerization - We have expertise in peptide design, and use solid phase peptide synthesis (SPPS ... Liposomes, protein purification, and antibody digestion. I. Fluorescent probes - Fluorescence remains the premier approach to ... Similar to siRNA or CRISPR-Cas9, a PROTAC can degrade a target protein via the native proteasomal machinery, without genetic ...
  • To investigate biochemical properties of bacterial RVTs, we conducted in vitro studies of recombinant HaRVT protein from the filamentous gliding bacterium Herpetosiphon aurantiacus (Chloroflexi). (mblwhoilibrary.org)
  • Generation of stable cell lines is a widely used technique for continuous recombinant protein production. (bioportfolio.com)
  • Our data support the future use of the Drosophila S2 cell and C-tag platform technologies to enable cGMP-compliant biomanufacture of other novel and "difficult-to-express" recombinant protein-based vaccines. (nature.com)
  • 1 Central to ongoing efforts to develop highly effective vaccines against malaria infection, disease, or transmission is the production of recombinant proteins for use as subunit vaccines. (nature.com)
  • Full length human recombinant protein of human GAS7 (NP_003635) produced in HEK293T cell. (origene.com)
  • One of the persistent problems involved in the recombinant production of desired proteins resides in the obtainment of proper processing and folding of the recombinant product. (google.com)
  • In particular, various recombinant hosts are thought to have differing environments with respect to the level of oxidation or reduction that normally is associated with the cellular components, resulting in recombinant proteins with disulfide bond compositions which differ from those of the native protein, and, in general, altered three-dimensional dimensional structures. (google.com)
  • Protein kinase CK2 links polyamine metabolism to MAPK signalling in Drosophila. (sdbonline.org)
  • Western immunoblot analysis revealed that both PRMT1 and PRMT5 proteins were expressed in the cytosol of liver and pancreas with molecular mass of about 42 kDa and 72 kDa, respectively. (nih.gov)
  • Human adenovirus was detected by real-time and conventional polymerase chain reaction and molecular typed by partial hexon capsid protein gene sequencing. (cdc.gov)
  • The two strengths of solution scattering are (i) the ability to produce molecular structures when crystals cannot be obtained (although only at medium structural resolution) and (ii) the validation of protein crystal structures in solution. (royalsocietypublishing.org)
  • To gain insights into the molecular mechanisms that govern the metalloreductase activity of STEAP proteins, we determine structures of human STEAP4 using single-particle cryo-EM. (nature.com)
  • some proteins may form dimers or multimers which increase the molecular weights. (origene.com)
  • In each instance, the Eib proteins were observed as multiple bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), some of the bands exceeding 200 kDa in apparent molecular mass ( 16 ). (asm.org)
  • Researchers at Juntendo University report in the journal Leukemia how mutants of the protein calreticulin lead to molecular mechanisms triggering myeloproliferative neoplasms, which can cause cancer. (news-medical.net)
  • Although more detailed molecular and structural analyses are required to understand the mechanism behind MPL activation by mutant CALR proteins, our findings shed light on MPN pathogenesis and provide support for the development of novel therapeutic strategies against MPNs with mutant CALR proteins. (news-medical.net)
  • Cooperation between the two termini appears to generate a unique conformational state, specifically recognized by thioflavin T, that promoted efficient multimerization of the oligomer into high-molecular-mass soluble complexes and dramatically enhanced resistance against trypsin digestion, enterotoxin activity of the diarrhea-inducing region (DIR), and double-layered particle-binding activity of the protein. (asm.org)
  • Each domain has a specific molecular composition, which includes protein complexes composed of distinct transmembrane, membrane-associated, and cytosolic components. (jci.org)
  • These are structurally conserved elements with unique molecular specificities that can be found in proteins of many different functions. (jci.org)
  • In the current study, we sought to determine the potential effects of tobacco smoke exposure on ADPN multimerization and secretion, as well as the relevant intracellular molecular mediators in these processes. (biomedcentral.com)
  • Biochemical properties of bacterial reverse transcriptase-related (rvt) gene products: multimerization, protein priming, and nucleotide preference. (mblwhoilibrary.org)
  • Fanconi anemia group G protein is a protein that in humans is encoded by the FANCG gene. (wikipedia.org)
  • DNA interstrand crosslinks are highly deleterious damages that are repaired by homologous recombination involving coordination of FA proteins and breast cancer susceptibility gene 1 (BRCA1), but the exact biochemical roles of these proteins is currently unclear. (wikipedia.org)
  • MCOLN2 (Mucolipin 2) is a Protein Coding gene. (genecards.org)
  • This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. (genecards.org)
  • XPO1 (Exportin 1) is a Protein Coding gene. (genecards.org)
  • The PA0943 gene was studied further and found to encode a soluble periplasmic protein important for XcpQ localization to the outer membrane. (asm.org)
  • alternative splicing may create proteins of different sizes from the same gene. (origene.com)
  • The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. (origene.com)
  • Fanconi anaemia, complementation group A, also known as FAA, FACA and FANCA, is a protein which in humans is encoded by the FANCA gene. (wikipedia.org)
  • The FANCA gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. (wikipedia.org)
  • The protein profiles led to the discovery of beneficial mutations affecting the malT gene, with strong genetic parallelism across independently evolved populations. (genetics.org)
  • This oxidoreductase protein is encoded by the gene EPX, expressed within these myeloid cells. (wikipedia.org)
  • In contrast, the rex gene product acts at the posttranscriptional level, permitting the expression of the viral structural proteins ( 37 , 45 ). (asm.org)
  • Consequently, the sizes of the Eib proteins appeared much greater than predicted from the gene sequences, implying stable multimerization. (asm.org)
  • Because the gene encoding the primary protein structure is generally expressed in a host system foreign to that in which it is normally transcribed and translated, the resultant product protein, while having the correct amino acid sequence, has different characteristics from the native protein. (google.com)
  • Two exons of the HP gene (encoding haptoglobin) exhibit copy number variation that affects HP protein structure and multimerization. (broadinstitute.org)
  • spFRET has been used to study dynamics of proteins, nucleic acids, and their complexes ( 4 - 6 ). (pnas.org)
  • Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids. (bioportfolio.com)
  • In higher plants thylakoids, the interactions lead to a lateral asymmetry in localization of protein complexes (i.e. granal/stromal thylakoids) that have been defined as a domain-like structures characteristic by different biochemical composition and function (Albertsson P-Å. (bioportfolio.com)
  • Our 3D confocal images captured heterogeneous distribution of all main photosynthetic pigment-protein complexes (PPCs), Photosystem I (fluorescently tagged by YFP), Photosystem II and Phycobilisomes. (bioportfolio.com)
  • Overexpression of Protein Complexes and Aneuploidy. (bioportfolio.com)
  • One snapshot of the peer review process for "Overdosage of Balanced Protein Complexes Reduces Proliferation Rate in Aneuploid Cells" (Chen et al. (bioportfolio.com)
  • Excitation-energy-transfer processes in pigment-protein complexes in photosynthetic organisms are often changed under different pH conditions. (bioportfolio.com)
  • Activated FANCD2 protein co-localizes with BRCA1 (breast cancer susceptibility protein) at ionizing radiation-induced foci and in synaptonemal complexes of meiotic chromosomes (see Figure: Recombinational repair of double strand damage). (wikipedia.org)
  • We discuss the importance of complementary data from analytical ultracentrifugation, and structural studies of protein-protein complexes. (royalsocietypublishing.org)
  • A key observation made by the scientists is that mutant CALR molecules form homomultimeric complexes (clusters of identical proteins), whereas non-mutant ('wild-type') CALRs do not. (news-medical.net)
  • PDZ domains have recently emerged as central organizers of protein complexes at the plasma membrane. (jci.org)
  • These include the structural basis for specificity of their binding interactions and ideas about how they organize both small local protein complexes used for signal transduction (transducisomes) and larger two-dimensional complexes like cell junctions and plasma membrane domains. (jci.org)
  • These protein complexes mediate the adhesive properties of particular cells, the formation of the paracellular barrier (tight junctions), ion transport, and transmission of signals between adjacent cells that regulate growth, differentiation, and homeostasis. (jci.org)
  • Formation of these protein complexes is determined in large part by the interactions of modular protein-binding domains. (jci.org)
  • These domains are, figuratively speaking, the glue that binds protein complexes together, and their unique specificity and regulated binding determine the distinct compositions of different complexes. (jci.org)
  • SCF complexes are a family of ubiquitin ligases composed of a common core of components and a variable component called an F-box protein that defines substrate specificity. (asm.org)
  • Distinct SCF complexes, defined by a particular F-box protein, target different substrate proteins for degradation. (asm.org)
  • Using Saccharomyces cerevisiae as a model system, we demonstrate that overproduction of polypeptides corresponding to the amino terminus of the F-box proteins Cdc4p and Met30p results in specific inhibition of their SCF complexes. (asm.org)
  • Thus, SCF complexes define a family of E3 ligases that may be distinguished by a particular F-box protein, which in turn determines substrate specificity for that complex. (asm.org)
  • Since ApCPEB binds to RNA, the researchers wanted to eliminate the possibility that the puncta were simply RNA protein complexes. (alzforum.org)
  • In summary, in rat hippocampus, NR2A and NR2B exist primarily in di-heteromeric complexes that interact similarly with PSD-95-related proteins but are associated with different protein complexes. (jneurosci.org)
  • The physiological role of the spliced form of X-box-binding protein 1 (XBP1s), a key transcription factor of the endoplasmic reticulum (ER) stress response, in adipose tissue remains largely unknown. (diabetesjournals.org)
  • Accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and leads to the activation of unfolded protein response (UPR). (diabetesjournals.org)
  • The protein is produced on ribosomes embedded on the surface of the endoplasmic reticulum, since it must be ultimately localized to the granules. (wikipedia.org)
  • Rotavirus NSP4 is a multifunctional endoplasmic reticulum (ER)-resident nonstructural protein with the N terminus anchored in the ER and about 131 amino acids (aa) of the C-terminal tail (CT) oriented in the cytoplasm. (asm.org)
  • We purchased polyclonal antibodies against mouse adiponectin and ERp44 (endoplasmic reticulum [ER] resident protein of 44 kDa) from Cell Signaling. (biomedcentral.com)
  • 2018. Stochastic protein multimerization, activity, and fitness. (asu.edu)
  • Remarkably, DNA binding by ParA and ParB as well as their interactions with protein partners conceivably may be modulated by intra- and extracellular conditions. (frontiersin.org)
  • The biologically active agent is preferably a protein, such as an extracellular soluble protein, e.g., an extracellular enzyme. (google.com)
  • The extracellular polymeric substance (EPS) matrix of P. aeruginosa biofilms has been widely characterized as being a variable combination of secreted polysaccharides that enhance surface attachment ( 3 ), extracellular DNA (eDNA) ( 4 ), and extracellular structural proteins ( 5 ). (asm.org)
  • The interaction of Wnt proteins with their receptors on the cell surface is the first step in transducing the extracellular signal into intracellular responses. (biologists.org)
  • Bacteria initially adhere to the cell membrane and extracellular matrix substrates through surface proteins (adhesins) [ 17 , 18 ] and are then internalized by different NPPCs. (hindawi.com)
  • Consistent with this, a PA0943 null mutation reduced the levels of type 2 secretion-dependent proteins in the culture supernatant. (asm.org)
  • Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts. (springer.com)
  • Secretion of micronemal proteins is associated with Toxoplasma invasion of host cells. (springer.com)
  • We report that Mycobacterium tuberculosis and Staphylococcus aureus secrete virulence factors via type VII protein secretion (T7S), a system that intriguingly requires all of its secretion substrates for activity. (osti.gov)
  • Exploiting the sequence divergence and distinct syncytiogenic rates of representative p10 FAST proteins from avian and bat reovirus isolates, we determined the p10 ectodomain is a compact, complex fusion module comprising two independent functional motifs. (prolekare.cz)
  • Araki and colleagues could attribute the multimerization to particular chemical motifs in the mutant molecules. (news-medical.net)
  • Although originally referred to as GLGF or DHR motifs, they are now known by an acronym representing these first three PDZ-containing proteins (PDZ: PSD95/DLG/ZO-1). (jci.org)
  • We demonstrate the utility of the method for conditionally expressible variants of Drosophila vAChT that utilize 7XMYC and 6XOLLAS epitope tags, but the strategy is generally applicable to any protein in any species. (g3journal.org)
  • In preparation for clinical trials, a full-length PfRH5 protein vaccine called "RH5.1" was produced as a soluble product under cGMP using the ExpreS 2 platform (based on a Drosophila melanogaster S2 stable cell line system). (nature.com)
  • PDZ domains were originally identified as conserved sequence elements within the postsynaptic density protein PSD95/SAP90, the Drosophila tumor suppressor dlg-A, and the tight junction protein ZO-1. (jci.org)
  • Multimerization of Drosophila sperm protein Mst77F causes a unique condensed chromatin structure. (mpg.de)
  • L ow-density-lipoprotein receptor- r elated p roteins 5 and 6 (Lrp5 and Lrp6) in vertebrates, and their Drosophila ortholog Arrow, are single-span transmembrane proteins that are indispensable for Wnt/β-catenin signaling, and are likely to act as Wnt co-receptors. (biologists.org)
  • Drosophila RSK influences the pace of the circadian clock by negative regulation of protein kinase Shaggy activity. (sdbonline.org)
  • Multifunctional RNA Processing Protein SRm160 Induces Apoptosis and Regulates Eye and Genital Development in Drosophila. (sdbonline.org)
  • The Drosophila RNA-binding protein HOW controls the stability of dgrasp mRNA in the follicular epithelium. (sdbonline.org)
  • protein -protein interactions. (bioportfolio.com)
  • As protein-protein interactions (PPI) require productive encounters among subunits, these structural variations are related in part to variation in cellular protein abundance. (aps.org)
  • Our work demonstrates the feasibility of rapidly generating small interaction networks via FACS-FRET and defines the network of intra-HCV protein interactions. (mcponline.org)
  • Thus, a promising strategy would be to specifically inhibit interactions among viral proteins. (mcponline.org)
  • Surprisingly, until now, a comprehensive analysis of the putative interactions and the interplay of HCV proteins with each other in living human cells has been lacking. (mcponline.org)
  • In the present work, we did an extensive and thorough analysis of intra-HCV protein interactions. (mcponline.org)
  • Because binding specificities are based on a few amino acid residues, these domains are uniquely suited to permit evolution of new protein interactions by coordinate mutations in the domain and target peptide sequence. (jci.org)
  • Isolated di-heteromeric receptor fractions were used to examine NR2A- or NR2B-specific interactions with synapse-associated proteins. (jneurosci.org)
  • Analysis of phosphorylation-dependent protein-protein interactions of histone H3. (mpg.de)
  • These proteins have three PDZ (for PSD-95, Dlg, ZO-1/Dlg-homologous region) domains, one Src homology 3 domain, and one guanylate kinase-like domain and are generically referred to as membrane-associated guanylate kinases (MAGUKs). (jneurosci.org)
  • Isoform 1 (1327 amino acids) contains region with homopolymeric runs of His, Pro, and Ser (HPS domain, aa 1-181), 15 ankyrin repeats (ANK 1 to 15, aa 215-934), multimerization domain homologous to the sterile alpha motif (SAM domain, aa 1030-1089) and PARP catalytic domain (aa 1112-1317). (atlasgeneticsoncology.org)
  • However, visualizing the expression of a protein within a given neuron is often challenging because most neurons are intricately intermingled with numerous other neurons, making individual neuronal expression difficult to discern, especially since many neuronal genes are expressed at low levels. (g3journal.org)
  • The most critical components of the Psp system are the PspF, PspA, PspB, and PspC proteins, encoded by adjacent genes. (asm.org)
  • As for every replication-competent retrovirus, the HTLV-1 genome contains the gag , pol , and env genes, which encode the viral structural proteins and enzymes. (asm.org)
  • The Tax protein activates the viral long terminal repeat promoter element, resulting in enhanced transcription of all viral genes ( 15 , 79 ). (asm.org)
  • The eib genes of Escherichia coli encode surface-exposed proteins which bind immunoglobulins (Ig) such as the Fc fragment of human IgG (IgG Fc) in a nonimmune manner. (asm.org)
  • By subcloning portions of the eibA and eibF genes, we have identified distinct sequence segments sufficient to cause Ig binding, multimerization, and discrimination between IgA and IgG. (asm.org)
  • The direct activator of transcription of the majority of V. cholerae virulence genes is the ToxT protein ( 5 ), which is a member of the large AraC/XylS family of transcriptional regulators ( 17 ). (asm.org)
  • As such, this platform, which links protein abundance, PPI and quaternary structure in growing and dividing cells can be extended to evolutionary models for the emergence and diversification of multimeric proteins. (aps.org)
  • We investigate cooperativity - a ubiquitous functional property of multimeric proteins - as a~possible selective force driving multimerization, demonstrating a reduction in the cost of protein production relative to the overall proteome energy budget that can be tied to fitness. (aps.org)
  • Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal ( NES ) and of RNAs. (rcsb.org)
  • IMPORTANCE The promyelocytic leukemia protein (PML) is a eukaryotic protein that can polymerize in discrete nuclear assemblies known as PML nuclear bodies (NBs) and plays essential roles in many different cellular processes. (asm.org)
  • Immunochemical study of mouse tissue indicates that FANCA is present at a higher level in lymphoid tissues, the testis and the ovary, and though the significance of this is unclear, it suggests that the presence of FA proteins might be related to cellular proliferation. (wikipedia.org)
  • This is proteolytically processed by cellular and viral proteases into structural (Core, E1, E2) and nonstructural (p7, NS2, NS3, NS4A/B, and NS5A/B) proteins ( 1 ). (mcponline.org)
  • An impressive body of data was derived from protein interaction or siRNA screens investigating the interplay of HCV proteins with cellular factors ( 3 ⇓ - 5 ). (mcponline.org)
  • However, it is also understood that the three-dimensional conformation of the protein may be different depending on the cellular environment. (google.com)
  • Autophagy is a major protein turnover pathway by which cellular components are delivered into the lysosomes for degradation and recycling. (frontiersin.org)
  • Although a few have been identified to be involved in important biological pathways, such as the cell division cycle and coordinating cellular responses to changes in environmental conditions, the role of the overwhelming majority of F-box proteins is not clear. (asm.org)
  • Cellular membranes comprise hundreds of different lipid species and at least as many different proteins. (portlandpress.com)
  • Cellular signals downstream of Src(527F) can regulate multimerization. (cdc.gov)
  • Of the three branches of mammalian UPR, inositol-requiring enzyme-1α (IRE1α) and its downstream target X-box-binding protein 1 (XBP1) are most highly conserved from yeast to humans ( 2 , 3 ). (diabetesjournals.org)
  • One of the first protein polymorphisms identified in humans involves the abundant blood protein haptoglobin. (broadinstitute.org)
  • The postsynaptic density (PSD), a highly organized cytoskeletal structure found adjacent to the postsynaptic membrane of excitatory synapses, is believed to play a role in the organization of receptors and related proteins involved in synaptic signaling. (jneurosci.org)
  • In the February 5 Cell, researchers led by Kausik Si at the Stowers Institute for Medical Research, Kansas City, Missouri, report that in the marine mollusk Aplysia Californica , prion-like activity of an RNA binding protein controls long-term facilitation (LTF), a strengthening of synaptic transmission that has been linked to memory formation. (alzforum.org)
  • Differential localization of NR2A- or NR2B-containing NMDARs within the neuron and subunit-specific protein associations may explain differences in NR2A and NR2B contributions to synaptic plasticity and excitotoxic cell death. (jneurosci.org)
  • However, NR2A-containing receptors coimmunoprecipitated a greater proportion of the synaptic proteins neuronal nitric oxide synthase, Homer, and β-catenin. (jneurosci.org)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • Mucolipins constitute a family of cation channel proteins with homology to the transient receptor potential superfamily. (genecards.org)
  • Mutant CALR, in turn, is known to promote the activation of another protein, thrombopoietin receptor, associated with the development of cancers. (news-medical.net)
  • Several studies have demonstrated that CMA is a highly regulated and degradative event, involving HSC70 (heat shock protein 70 complex) and multimerization of the LAMP2A receptor (lysosome-associated membrane protein type 2A). (frontiersin.org)
  • These results indicate that individual receptor-associated proteins may have specific functions that are critical to synapse development. (jneurosci.org)
  • Newly synthesized IL-6 accumulates in the Golgi complex and exits in tubulovesicular carriers either as the sole labeled cargo or together with TNFα, utilizing specific soluble NSF attachment protein receptor (SNARE) proteins to fuse with the recycling endosome. (rupress.org)
  • Antibodies raised against receptor-binding domain of Plasmodium knowlesi Duffy binding protein inhibit erythrocyte invasion. (springer.com)
  • Also found in complex with several viral proteins involved in RNAs' nuclear export like HIV-1 Rev and HTLV-1 Rex. (rcsb.org)
  • The HCV genome is translated into a polyprotein which is proteolytically processed into 10 viral proteins. (mcponline.org)
  • however, it remains unclear how viral proteins interact with each other. (mcponline.org)
  • In the nucleus, in association with RAN BP3 , binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). (rcsb.org)
  • It binds to the HCV capsid protein Core and the two glycoproteins E1 and E2. (mcponline.org)
  • Mechanistically, XBP1s promotes adiponectin multimerization rather than activating its transcription, likely through a direct regulation of the expression of several ER chaperones involved in adiponectin maturation, including glucose-regulated protein 78 kDa, protein disulfide isomerase family A, member 6, ER protein 44, and disulfide bond oxidoreductase A-like protein. (diabetesjournals.org)
  • The antibody against DsbA-L (disulfide-bond A oxidoreductase-like protein) was from Abcam. (biomedcentral.com)
  • In this regard, merozoite proteins involved in RBC invasion have traditionally been targeted via the induction of growth inhibitory antibodies, 3 however historical candidate antigens have suffered from substantial levels of polymorphism and redundancy, leading to non-protective or strain-specific vaccine-induced antibody responses. (nature.com)
  • Dopamine beta hydroxylase antibody detects DBH protein at cytosol on U87 xenograft by immunohistochemical analysis. (origene.com)
  • 2019. Evolution of protein interfaces in multimers and fibrils. (asu.edu)
  • For some neuronal proteins this information may include neurotransmitter (NT) usage and sites of NT release. (g3journal.org)
  • However, this strategy often exposes the problem of sensitivity, especially for proteins with low endogenous levels of expression as is common with many neuronal proteins. (g3journal.org)
  • In this study, we show that overexpression of XBP1s promotes adiponectin multimerization in adipocytes, thereby regulating systemic glucose homeostasis. (diabetesjournals.org)
  • Thus, we conclude that XBP1s is an important regulator of adiponectin multimerization, which may lead to a new therapeutic approach for the treatment of type 2 diabetes and hypoadiponectinemia. (diabetesjournals.org)
  • Defects in adiponectin multimerization are associated with hypoadiponectinemia and hyperglycemia ( 15 ). (diabetesjournals.org)
  • One motif determines species-specific p10 fusion efficiency by governing formation of a cystine loop fusion peptide, while the other directs reversible clustering and multimerization of p10 in cholesterol-dependent membrane microdomains. (prolekare.cz)
  • The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. (wikipedia.org)
  • To undergo CMA degradation, proteins must contain a KFERQ motif in their amino acid sequences, which is necessary to bind the chaperone HSC70 ( 10 , 11 ). (frontiersin.org)
  • Recent crystallographic analyses have confirmed a wealth of genetic and biochemical evidence by demonstrating that the ∼40-amino-acid F-box motif links the F-box protein with the common SCF components by binding Skp1p ( 35 , 49 ). (asm.org)
  • Jeff Kieft (Colorado) presented a translation initiation motif that may enable evolutionary exploration of new translated proteins. (smbe.org)
  • In the assay, the reporter construct, carrying one half of the luciferase protein, is targeted to lipid microdomains through the fused acetylation motif from Src-family kinase Fyn. (portlandpress.com)
  • Deletion of the leucine zipper motif or PKC phosphorylation affected AFAP-110's conformation, which correlated with changes in multimerization and increased the capability of rAFAP-110 to cross-link actin filaments. (cdc.gov)
  • The principle underlying VLP vaccines is the induction of neutralizing antibodies that block virus infection by binding to surface L1 protein loops that are highly heterogeneous among different HPV types ( 9 - 12 ). (asm.org)
  • In recent years, a greater range of proteins have been successfully studied by scattering, including antibodies of adaptive immunity and the complement proteins of innate immunity. (royalsocietypublishing.org)
  • The complex was recognized by antibodies to monomeric ApCPEB, and it was also resistant to boiling in 10 percent sodium dodecyl sulfate, consistent with it being a stable protein aggregate and not an RNA/protein mixture. (alzforum.org)
  • Slow-clearing, tumor-targeting proteins such as monoclonal antibodies typically exhibit high tumor accumulation but low tissue contrast, whereas intermediate-sized proteins such as scFvs show faster clearance but only moderate tumor accumulation. (aacrjournals.org)
  • Slow-clearing, tumor-targeting proteins such as monoclonal antibodies with long half-lives typically exhibit high tumor accumulation but low tissue contrast ( 8 ). (aacrjournals.org)
  • Recently, we have developed combinatorial libraries of a new class of small proteins, termed "designed ankyrin repeat proteins" ( 19 , 20 ) that can act as an alternative to antibodies, as they are particularly robust to engineering. (aacrjournals.org)
  • For this purpose we cross-linked glycosyl-phosphatidylinositol (GPI)-anchored proteins placental alkaline phosphatase (PLAP), Thy-1, influenza virus hemagglutinin (HA), and the raft lipid ganglioside GM1 using antibodies and/or cholera toxin. (rupress.org)
  • Gilbert, H. F., Catalysis of thiol/disulfide exchange: singleturnover reduction of protein disulfide-isomerase by glutathione and catalysis of peptide disulfide reduction. (kisti.re.kr)
  • Remarkably, a juxtamembrane tetra-peptide is solely responsible for co-dependent clustering and multimerization of p10 in distinct, species-specific fusion platforms. (prolekare.cz)
  • V. Peptide optimization and multimerization - We have expertise in peptide design, and use solid phase peptide synthesis (SPPS) to investigate SAR of sequences of interest to increase potency, metabolic stability, and water solubility. (nih.gov)
  • Previous studies showed a peptide spanning aa 114 to 135 to induce diarrhea in newborn mouse pups with the 50% diarrheal dose approximately 100-fold higher than that for the full-length protein, suggesting a role for other regions in the protein in potentiating its diarrhea-inducing ability. (asm.org)
  • Furthermore, a peptide spanning amino acids (aa) 114 to 135 was reported to induce diarrhea at an approximately 100-fold molar excess compared to the full-length protein ( 6 ). (asm.org)
  • However, the coiled-coil domain lacks the information necessary to predict a structural basis for rotavirus virulence, as a peptide from this region exhibited highly reduced diarrhea-inducing potential compared to the full-length protein ( 6 ). (asm.org)
  • Schematic diagram of a vertebrate polarized epithelial cell, showing the distinct subcellular localization of several epithelial PDZ proteins into the apical, basal-lateral, and junctional domains. (jci.org)
  • Central to these processes is the timely engagement and disengagement of specific receptors upon the regulated release of apical invasion proteins from parasite secretory organelles (micronemes, rhoptries). (springer.com)
  • In this short review, we summarise recent progress on identification and functional characterisation of apical invasion proteins mobilised to the parasite surface from the microneme organelles. (springer.com)
  • Melzer, J., Kraft, K. F., Urbach, R., Raabe, T. (2013) The p21-activated kinase Mbt is a component of the apical protein complex in central brain neuroblasts and controls cell proliferation. (sdbonline.org)
  • In polarized epithelial cells the apical and basolateral plasma membrane strongly differ in lipid and protein composition ( Rodriguez-Boulan and Nelson, 1989 ). (rupress.org)
  • A second proinflammatory cytokine produced by activated macrophages is interleukin 6 (IL-6), which, lacking a transmembrane domain, is trafficked and secreted directly as a soluble protein. (rupress.org)
  • This epitope is conserved among different papillomaviruses (PV) and contains two cysteine residues that are present in the L2 proteins of all known PV types. (asm.org)
  • This major cross-neutralizing epitope, which we mapped to the aa 20 to 38 region of L2 (L2 20-38 ), contains two cysteine residues (positions 22 and 28) that are conserved in the L2 proteins of all known PVs. (asm.org)
  • FANCA proteins are involved in inter-strand DNA cross-link repair and in the maintenance of normal chromosome stability that regulates the differentiation of haematopoietic stem cells into mature blood cells. (wikipedia.org)
  • This interaction has been proposed as essential for the stability and nuclear localization of FA core complex proteins. (wikipedia.org)
  • Treatment of basic fibroblast growth factor (bFGF) with organic disulfides, preferably glutathione disulfide, or with inorganic compounds of similar function results in a bFGF composition of enhanced stability and resistance to multimerization. (google.com)
  • The outcome of the most intensively studied Wnt pathway - the canonical Wnt/β-catenin signaling pathway - relies to a large extent on the regulation of the stability/abundance of the β-catenin protein ( Fig. 1 ). (biologists.org)
  • The FAST proteins are the only known examples of nonenveloped virus membrane fusion proteins, and they are the smallest known viral fusogens whose structural and functional attributes are incompatible with current models of protein-mediated membrane fusion. (prolekare.cz)
  • CALR is a protein capable of binding to other, misfolded proteins, triggering their degradation. (news-medical.net)
  • Luminal acidification is essential for the delivery and degradation of internalized ligands in lysosomes and regulates posttranslational modification and sorting of proteins along the endosomal pathway ( 56 ). (physiology.org)
  • Ubiquitin ligases direct the transfer of ubiquitin onto substrate proteins and thus target the substrate for proteasome-dependent degradation. (asm.org)
  • With a wide range of proteins being targeted for degradation by the proteasome, the cell overcomes the problem of specificity through a hierarchical order of Ub-conjugating machinery. (asm.org)
  • Current commercial prophylactic human papillomavirus (HPV) vaccines are based on virus-like particles assembled from the major capsid protein L1 and show excellent safety and efficacy profiles. (asm.org)
  • In contrast, the minor capsid protein L2 contains a so-called major cross-neutralizing epitope that can induce broad-range protective responses against multiple HPV types. (asm.org)
  • They are virus-like particle (VLP) vaccines based on the L1 major capsid protein. (asm.org)
  • In contrast to L1, the minor capsid protein L2 contains a number of conserved epitopes that are targets for virus neutralization ( 13 - 15 ). (asm.org)
  • The protein specifically inhibits the nuclear export of Rev and U snRNAs. (genecards.org)
  • this interaction specifically inhibits TRIM25 multimerization and TRIM25 -mediated DDX58 CARD ubiquitination. (rcsb.org)
  • The activation of TRP channels may be directly mediated by physical or chemical stimuli or indirectly by second messengers generated by G protein-coupled or tyrosine kinase receptors. (physiology.org)
  • PARsylation activity of TNKS increases after phosphorilation by mitogen-activated protein kinase (MAPK) upon insulin stimulation (Chi and Lodish, 2000). (atlasgeneticsoncology.org)
  • A role for coccidian cGMP-dependent protein kinase in motility and invasion. (springer.com)
  • Importantly, patches of GPI-anchored PLAP accumulated src-like protein tyrosine kinase fyn, which is thought to be anchored in the cytoplasmic leaflet of raft domains. (rupress.org)
  • We demonstrate favorable agreement between the model and a wide class of proteins using E. coli proteome data. (aps.org)
  • They also suggest that it "might be representative of a new class of proteins that utilize a self-perpetuating multimeric state to create self-sustaining altered activity state in the cell in response to specific stimuli. (alzforum.org)
  • The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. (nih.gov)
  • The FANCA protein is composed of 1455 amino acids. (wikipedia.org)
  • Eosinophil peroxidase is a heme peroxidase, its activities including the oxidation of halide ions to bacteriocidal reactive oxygen species, the cationic disruption of bacterial cell walls, and the post-translational modification of protein amino acid residues. (wikipedia.org)
  • Three protein domains which mediate nuclear import-RNA binding, nuclear export, and Rex oligomerization have been mapped within the 189-amino-acid Rex polypeptide. (asm.org)
  • In this report, employing a large number of methods and deletion and amino acid substitution mutants, we provide evidence for the cooperation between the extreme C terminus and a putative amphipathic α-helix located between aa 73 and 85 (AAH 73-85 ) at the N terminus of ΔN72, a mutant that lacked the N-terminal 72 aa of nonstructural protein 4 (NSP4) from Hg18 and SA11. (asm.org)
  • These 80-90 amino acids sequences have now been identified in well over 75 proteins and are characteristically expressed in multiple copies within a single protein. (jci.org)
  • Clinical experience with all HIV-1 agents has clearly demonstrated the ability of HIV-1 to easily evade the antiviral effects of any monotherapeutic drug administration strategy through the rapid accumulation of amino acid changes in the targeted proteins-reverse transcriptase, protease, envelope, and integrase [ 5 ]. (hindawi.com)
  • In recent years, the combination of high resolution microscopy and live cell imaging of fluorescently tagged proteins has largely redefined the post-Golgi trafficking and sorting of biosynthetic cargo in polarized and nonpolarized cells. (rupress.org)
  • The FA proteins interact through a multiprotein pathway. (wikipedia.org)
  • In addition, both ParA and ParB were shown to interact with the other proteins, including those involved in cell division or cell elongation. (frontiersin.org)
  • The CT-A subunit catalyzes ADP-ribosylation of a Gα protein, resulting in elevated levels of cyclic AMP. (asm.org)
  • To establish the formation of Tg multimers, the partially unfolded/reduced Tg or deoxycholate-treated/ reduced Tg was subjected to protein disulfide isomerase (PDI)-mediated multimerization. (kisti.re.kr)
  • This family of surface-exposed proteins shares several features, such as the ability to impart resistance to human serum complement and a tendency to exist as stable multimers. (asm.org)
  • The Eib proteins have several phenotypic features in common with these proteins, such as the ability to impart resistance to human serum complement and a tendency to exist as highly stable multimers. (asm.org)
  • Interestingly, not all proteins are able to be CMA substrates. (frontiersin.org)
  • Creating inhibitors that will block the in vivo activities of specific SCF ubiquitin ligases may provide identification of substrates of these uncharacterized F-box proteins. (asm.org)
  • May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway (PubMed:17662026). (genecards.org)
  • In cells, FANCA involvement in this 'FA core complex' is required for the activation of the FANCD2 protein to a monoubiquitinated isoform (FANCD2-Ub) in response to DNA damage, catalysing activation of the FA/BRCA DNA damage-response pathway, leading to repair. (wikipedia.org)
  • The endosomal pathway is recognized as a key regulator of endocytic membrane traffic, protein sorting, and signaling. (physiology.org)
  • The intracellular trafficking capabilities of wild-type and mutant Rex proteins reveal that biologically inactive and multimerization-deficient Rex mutants are still efficiently translocated from the nucleus to the cytoplasm. (asm.org)
  • We show here that with very small high-affinity proteins such as designed ankyrin repeat proteins (DARPins), these limits can be overcome. (aacrjournals.org)
  • We show here that with very small high-affinity proteins such as designed ankyrin repeat proteins (DARPins), these limits can be overcome, and we present a systematic approach that establishes the observed tumor accumulation and targeting contrast as a function of affinity, targeting molecule size, and pharmacokinetics. (aacrjournals.org)
  • ATP hydrolysis by XylR, the cognate regulator of the system, is preceded by the multimerization of XylR at the enhancer, which is itself triggered by the sole allosteric effect of ATP binding to the protein. (nih.gov)
  • We also discuss the applications to the large multi-domain proteins of the complement system, most notably its major regulator factor H, which is important in age-related macular degeneration and renal diseases. (royalsocietypublishing.org)
  • Other redox-sensitive residues, such as surface-exposed Cys247, mediate GAPDH multimerization and contribute to noncanonical functions. (bloodjournal.org)
  • Secretins are bacterial outer membrane proteins that are important for protein export. (asm.org)
  • However, they can also mislocalize and cause stress to the bacterial cell, which is dealt with by the well-conserved phage shock protein (Psp) system in a highly specific manner. (asm.org)
  • Chemical cross‐linking studies of purified and surface‐exposed invasin proteins, and the dominant‐interfering effect of a non‐functional invasin derivative are consistent with the presence of a self‐association domain that is located within the region of invasin that enhances bacterial uptake. (embopress.org)
  • Activated FANCD2 protein may function prior to the initiation of meiotic recombination, perhaps to prepare chromosomes for synapsis, or to regulate subsequent recombination events. (wikipedia.org)
  • In addition to structural proteins, HTLV-1 encodes at least two trans -regulatory proteins, Tax and Rex, which are essential for virus replication (reviewed in references 18 , 29 , and 78 ). (asm.org)
  • Within recycling endosomes, we demonstrate the compartmentalization of cargo proteins, wherein IL-6 is dynamically segregated from TNFα and from surface recycling transferrin. (rupress.org)
  • The membrane-associated guanylate kinases [Chapsyn-110/postsynaptic density-93 (PSD-93), synapse-associated protein-90 (SAP-90)/PSD-95, and SAP-102] are believed to cluster and anchor NMDA receptors at the synapse and to play a role in signal transduction. (jneurosci.org)
  • Our results indicate that NR2A- or NR2B-containing NMDARs associate similarly with postsynaptic density-95 (PSD-95), synapse-associated protein 102, and PSD-93 at P42. (jneurosci.org)
  • Some membrane components, such as cholesterol and sphingomyelin attract each other, segregating laterally to form domains whose lipid and protein composition differs from the rest of the membrane [ 1 ]. (portlandpress.com)
  • Whereas protein-ligand binding affinities have long established prominence, binding rate constants and binding mechanisms have continued to gain increasing attention in recently years. (aps.org)
  • Here, we address the question of whether Trx scaffold multimerization may further enhance the immunogenicity of the TrxL2 vaccine. (asm.org)
  • The calcium site serves not only as a scaffold for protein folding, but also for proper association of the two chains. (wikipedia.org)
  • Soldati D, Dubremetz JF, Lebrun M. Microneme proteins: Structural and functional requirements to promote adhesion and invasion by the apicomplexan parasite Toxoplasma gondii. (springer.com)
  • RNAs - Physical and functional modulators of chromatin reader proteins. (mpg.de)