The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Attachment Sites, Microbiological
The mechanism by which latent viruses, such as genetically transmitted tumor viruses (PROVIRUSES) or PROPHAGES of lysogenic bacteria, are induced to replicate and then released as infectious viruses. It may be effected by various endogenous and exogenous stimuli, including B-cell LIPOPOLYSACCHARIDES, glucocorticoid hormones, halogenated pyrimidines, IONIZING RADIATION, ultraviolet light, and superinfecting viruses.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
SOS Response (Genetics)
An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive.
Sequence Analysis, DNA
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Escherichia coli O157
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
Shiga Toxin 2
Open Reading Frames
Vibrio cholerae O1
Gene Transfer, Horizontal
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
Interspersed Repetitive Sequences
Copies of transposable elements interspersed throughout the genome, some of which are still active and often referred to as "jumping genes". There are two classes of interspersed repetitive elements. Class I elements (or RETROELEMENTS - such as retrotransposons, retroviruses, LONG INTERSPERSED NUCLEOTIDE ELEMENTS and SHORT INTERSPERSED NUCLEOTIDE ELEMENTS) transpose via reverse transcription of an RNA intermediate. Class II elements (or DNA TRANSPOSABLE ELEMENTS - such as transposons, Tn elements, insertion sequence elements and mobile gene cassettes of bacterial integrons) transpose directly from one site in the DNA to another.
Distinct units in some bacterial, bacteriophage or plasmid GENOMES that are types of MOBILE GENETIC ELEMENTS. Encoded in them are a variety of fitness conferring genes, such as VIRULENCE FACTORS (in "pathogenicity islands or islets"), ANTIBIOTIC RESISTANCE genes, or genes required for SYMBIOSIS (in "symbiosis islands or islets"). They range in size from 10 - 500 kilobases, and their GC CONTENT and CODON usage differ from the rest of the genome. They typically contain an INTEGRASE gene, although in some cases this gene has been deleted resulting in "anchored genomic islands".
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Microscopy, Electron, Transmission
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Rec A Recombinases
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
Viral Regulatory and Accessory Proteins
A broad category of viral proteins that play indirect roles in the biological processes and activities of viruses. Included here are proteins that either regulate the expression of viral genes or are involved in modifying host cell functions. Many of the proteins in this category serve multiple functions.
A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Gene Expression Regulation, Bacterial
Escherichia coli K12
Integration Host Factors
An ENTEROTOXIN from VIBRIO CHOLERAE. It consists of two major protomers, the heavy (H) or A subunit and the B protomer which consists of 5 light (L) or B subunits. The catalytic A subunit is proteolytically cleaved into fragments A1 and A2. The A1 fragment is a MONO(ADP-RIBOSE) TRANSFERASE. The B protomer binds cholera toxin to intestinal epithelial cells, and facilitates the uptake of the A1 fragment. The A1 catalyzed transfer of ADP-RIBOSE to the alpha subunits of heterotrimeric G PROTEINS activates the production of CYCLIC AMP. Increased levels of cyclic AMP are thought to modulate release of fluid and electrolytes from intestinal crypt cells.
Amino Acid Sequence
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Gene Expression Regulation, Viral
Salmonella paratyphi C
Genetic Complementation Test
Shiga-Toxigenic Escherichia coli
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Operator Regions, Genetic
Enterohemorrhagic Escherichia coli
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Sequence Homology, Nucleic Acid
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.
RNA, Transfer, Arg
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.
Inverted Repeat Sequences
Copies of nucleic acid sequence that are arranged in opposing orientation. They may lie adjacent to each other (tandem) or be separated by some sequence that is not part of the repeat (hyphenated). They may be true palindromic repeats, i.e. read the same backwards as forward, or complementary which reads as the base complement in the opposite orientation. Complementary inverted repeats have the potential to form hairpin loop or stem-loop structures which results in cruciform structures (such as CRUCIFORM DNA) when the complementary inverted repeats occur in double stranded regions.
Centrifugation, Density Gradient
Promoter Regions, Genetic
Bacterial Typing Techniques
Shiga Toxin 1
Viral Tail Proteins
Electrophoresis, Gel, Pulsed-Field
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
Drug Resistance, Microbial
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Factor For Inversion Stimulation Protein
A highly abundant DNA binding protein whose expression is strongly correlated with the growth phase of bacteria. The protein plays a role in regulating DNA topology and activation of RIBOSOMAL RNA transcription. It was originally identified as a factor required for inversion stimulation by the Hin recombinase of SALMONELLA and Gin site-specific recombinase of BACTERIOPHAGE MU.
Sequence Homology, Amino Acid
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
RNA, Transfer, Amino Acid-Specific
Deoxyribonucleases, Type II Site-Specific
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 184.108.40.206.
Drug Resistance, Bacterial
Artificial Gene Fusion
Bacterial Outer Membrane Proteins
Oligonucleotide Array Sequence Analysis
Site-Specific DNA-Methyltransferase (Adenine-Specific)
An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 220.127.116.11.
Inhibition of spontaneous induction of lambdoid prophages in Escherichia coli cultures: simple procedures with possible biotechnological applications. (1/408)BACKGROUND: Infections of bacterial cultures by bacteriophages are serious problems in biotechnological laboratories. Apart from such infections, prophage induction in the host cells may also be dangerous. Escherichia coli is a commonly used host in biotechnological production, and many laboratory strains of this bacterium harbour lambdoid prophages. These prophages may be induced under certain conditions leading to phage lytic development. This is fatal for further cultivations as relatively low, though still significant, numbers of phages may be overlooked. Thus, subsequent cultures of non-lysogenic strains may be infected and destroyed by such phage. RESULTS: Here we report that slow growth of bacteria decreases deleterious effects of spontaneous lambdoid prophage induction. Moreover, replacement of glucose with glycerol in a medium stimulates lysogenic development of the phage after infection of E. coli cells. A plasmid was constructed overexpressing the phage 434 cI gene, coding for the repressor of phage promoters which are necessary for lytic development. Overproduction of the cI repressor abolished spontaneous induction of the lambda(imm434) prophage. CONCLUSIONS: Simple procedures that alleviate problems with spontaneous induction of lambdoid prophage and subsequent infection of E. coli strains by these phages are described. Low bacterial growth rate, replacement of glucose with glycerol in a medium and overproduction of the cI repressor minimise the risk of prophage induction during cultivation of lysogenic bacteria and subsequent infection of other bacterial strains. (+info)
Pilot study of the genetic diversity of the pneumococcal nasopharyngeal flora among children attending day care centers. (2/408)A pilot study was conducted to determine the genetic diversity of multiple colonies of pneumococci recovered from 37 nasopharyngeal (NP) samples of children. A total of 239 pneumococcal isolates (typically, six to eight colonies per sample) were typed by pulsed-field gel electrophoresis (PFGE). In most NP samples (89%) the multiple colonies shared common PFGE types and serotypes. However, four samples were heterogeneous (samples A through D): each contained two strains with different PFGE types, antibiotypes, and serotypes. Samples A and B each contained one strain of a vaccine capsular type and another expressing a non-vaccine type (according to the currently licensed seven-valent conjugate vaccine). In samples B and C the penicillin MIC for one strain was elevated and the other strain was susceptible. In each of the heterogeneous samples, one of the strains was a representative of an internationally disseminated clone. Samples A, C, and D contained strains which carried prophages that were inducible by mitomycin C and that could be visualized by electron microscopy. The comC gene allele (which encodes the competence-stimulating peptide) was the same in both strains found in each of samples A, B, and D. Carriage of multiple pneumococci with distinct properties should favor genetic exchange and provide a dynamic population structure for pneumococci in their ecological reservoir. Quantitative resolution of majority and minority components of the pneumococcal NP flora will be of importance for evaluation of the impact of intervention strategies such as vaccination or introduction of new antimicrobial agents. (+info)
Phenotypes of lexA mutations in Salmonella enterica: evidence for a lethal lexA null phenotype due to the Fels-2 prophage. (3/408)The LexA protein of Escherichia coli represses the damage-inducible SOS regulon, which includes genes for repair of DNA. Surprisingly, lexA null mutations in Salmonella enterica are lethal even with a sulA mutation, which corrects lexA lethality in E. coli. Nine suppressors of lethality isolated in a sulA mutant of S. enterica had lost the Fels-2 prophage, and seven of these (which grew better) had also lost the Gifsy-1 and Gifsy-2 prophages. All three phage genomes included a homologue of the tum gene of coliphage 186, which encodes a LexA-repressed cI antirepressor. The tum homologue of Fels-2 was responsible for lexA lethality and had a LexA-repressed promoter. This basis of lexA lethality was unexpected because the four prophages of S. enterica LT2 are not strongly UV inducible and do not sensitize strains to UV killing. In S. enterica, lexA(Ind(-)) mutants have the same phenotypes as their E. coli counterparts. Although lexA null mutants express their error-prone DinB polymerase constitutively, they are not mutators in either S. enterica or E. coli. (+info)
The Shiga-toxin VT2-encoding bacteriophage varphi297 integrates at a distinct position in the Escherichia coli genome. (4/408)The plaque-forming VT2-encoding lambdoid bacteriophage varphi297 was isolated from a Belgian clinical Escherichia coli O157:H7 isolate. PCR walking, starting from the int gene of phage varphi297, demonstrated that the varphi297 prophage integrated in the yecE gene of a lysogenic E. coli K12 strain. This integration site, in E. coli K12 and in the original clinical O157:H7 isolate, was confirmed by PCR using primers flanking this site. The excisionase protein of phage varphi297 is identical to the excisionase of VT1-encoding phage VT1-Sakai, while the integrases, which are 82% identical, show significant sequence divergence in the central and C-terminal region. This can explain the different integration sites of both prophages. The activity of the integrase was proven by its ability to mediate the integration of a suicide plasmid, carrying the attachment site of varphi297, at the appropriate position in the E. coli chromosome. (+info)
Transcription analysis of Streptococcus thermophilus phages in the lysogenic state. (5/408)The transcription of prophage genes was studied in two lysogenic Streptococcus thermophilus cells by Northern blot and primer-extension experiments. In the lysogen containing the cos-site phage Sfi21 only two gene regions of the prophage were transcribed. Within the lysogeny module an 1.6-kb-long mRNA started at the promoter of the phage repressor gene and covered also the next two genes, including a superinfection exclusion (sie) gene. A second, quantitatively more prominent 1-kb-long transcript was initiated at the promoter of the sie gene. Another prophage transcript of 1.6-kb length covered a group of genes without database matches that were located between the lysin gene and the right attachment site. The rest of the prophage genome was transcriptionally silent. A very similar transcription pattern was observed for a S. thermophilus lysogen containing the pac-site phage O1205 as a prophage. Prophages from pathogenic streptococci encode virulence genes downstream of the lysin gene. We speculate that temperate phages from lactic streptococci also encode nonessential phage genes ("lysogenic conversion genes") in this region that increase the ecological fitness of the lysogen to further their own evolutionary success. A comparative genome analysis revealed that many temperate phages from low GC content Gram-positive bacteria encode a variable number of genes in that region and none was linked to known phage-related function. Prophages from pathogenic streptococci encode toxin genes in this region. In accordance with theoretical predictions on prophage-host genome interactions a prophage remnant was detected in S. thermophilus that had lost most of the prophage DNA while transcribed prophage genes were spared from the deletion process. (+info)
Genome analysis of an inducible prophage and prophage remnants integrated in the Streptococcus pyogenes strain SF370. (6/408)The mitomycin C inducible prophage SF370.1 from the highly pathogenic M1 serotype Streptococcus pyogenes isolate SF370 showed a 41-kb-long genome whose genetic organization resembled that of SF11-like pac-site Siphoviridae. Its closest relative was prophage NIH1.1 from an M3 serotype S. pyogenes strain, followed by S. pneumoniae phage MM1 and Lactobacillus phage phig1e, Listeria phage A118, and Bacillus phage SPP1 in a gradient of relatedness. Sequence similarity with the previously described prophages SF370.2 and SF370.3 from the same polylysogenic SF370 strain were mainly limited to the tail fiber genes. As in these two other prophages, SF370.1 encoded likely lysogenic conversion genes between the phage lysin and the right attachment site. The genes encoded the pyrogenic exotoxin C of S. pyogenes and a protein sharing sequence similarity with both DNases and mitogenic factors. The screening of the SF370 genome revealed further prophage-like elements. A 13-kb-long phage remnant SF370.4 encoded lysogeny and DNA replication genes. A closely related prophage remnant was identified in S. pyogenes strain Manfredo at a corresponding genome position. The two prophages differed by internal indels and gene replacements. Four phage-like integrases were detected; three were still accompanied by likely repressor genes. All prophage elements were integrated into coding sequences. The phage sequences complemented the coding sequences in all cases. The DNA repair genes mutL and mutS were separated by the prophage remnant SF370.4; prophage SF370.1 and S. pneumoniae phage MM1 integrated into homologous chromosomal locations. The prophage sequences were interpreted with a hypothesis that predicts elements of cooperation and an arms race between phage and host genomes. (+info)
Use of real-time quantitative PCR for the analysis of phiLC3 prophage stability in lactococci. (7/408)Bacteriophages are a common and constant threat to proper milk fermentation. It has become evident that lysogeny is widespread in lactic acid bacteria, and in this work the temperate lactococcal bacteriophage phi LC3 was used as a model to study prophage stability in lactococci. The stability was analyzed in six phi LC3 lysogenic Lactococcus lactis subsp. cremoris host strains when they were growing at 15 and 30 degrees C. In order to perform these analyses, a real-time PCR assay was developed. The stability of the phi LC3 prophage was found to vary with the growth phase of its host L. lactis IMN-C1814, in which the induction rate increased during the exponential growth phase and reached a maximum level when the strain was entering the stationary phase. The maximum spontaneous induction frequency of the phi LC3 prophage varied between 0.32 and 9.1% (28-fold) in the six lysogenic strains. No correlation was observed between growth rates of the host cells and the spontaneous prophage induction frequencies. Furthermore, the level of extrachromosomal phage DNA after induction of the prophage varied between the strains (1.9 to 390%), and the estimated burst sizes varied up to eightfold. These results show that the host cells have a significant impact on the lytic and lysogenic life styles of temperate bacteriophages. The present study shows the power of the real-time PCR technique in the analysis of temperate phage biology and will be useful in work to reveal the impact of temperate phages and lysogenic bacteria in various ecological fields. (+info)
Genesis of variants of Vibrio cholerae O1 biotype El Tor: role of the CTXphi array and its position in the genome. (8/408)The gene encoding cholera toxin, the principal virulence factor of Vibrio cholerae, is encoded by a filamentous, lysogenic bacteriophage known as CTXphi. The genome of V. cholerae, the host for CTXphi, consists of two chromosomes, one large and one small. Here, it is shown that localization and array of CTX prophage DNA in either the large or small chromosome of V. cholerae is likely to be one of the reasons for the emergence of O1 biotype El Tor variants isolated just before and after the V. cholerae O139 cholera outbreak in 1992. Analyses of the organization of the CTX region of the genome of pre-O139 El Tor strains revealed that these strains carry two distinct CTX prophages integrated in the small chromosome in tandem: CTX(ET), the prophage having a conserved NotI site in its repeat sequence segment which seems to be specific for the El Tor strains so far examined, followed by CTX(calc)-like genome, the prophage found in recent O139 clinical isolates from Calcutta. In sharp contrast, in post-O139 El Tor strains only one copy of the CTX(ET) prophage was found to be integrated in the large chromosome. To the authors' knowledge, the presence of CTX prophage in the small chromosome of O1 El Tor strains has not been reported previously. It is also shown that the difference in the CTX copy number and the position of the bacteriophage on the genomes of pre- and post-O139 El Tor strains have an effect on cholera toxin production. While a pre-O139 strain produced maximum cholera toxin in yeast extract/peptone medium at 30 degrees C, a post-O139 El Tor strain showed maximal yield at 37 degrees C, indicating differential regulation of cholera toxin between the strains. It appears from this study that the variation in the integration site of the CTX prophage, its copy number and the presence of diverse phage genomes in V. cholerae O1 biotype El Tor may be strategically important for generating variants with subtle phenotypic modulations of virulence factor production in this longest-ruling seventh pandemic strain. (+info)
Prophages and satellite prophages are widespread among Streptococcus species and may play a role in pneumococcal pathogenesis -...
Abstract Prophages (viral genomes integrated within a host bacterial genome) are abundant within the bacterial world and are of interest because they often confer various phenotypic traits to their hosts, such as by encoding genes that increase pathogenicity. Satellite prophages are parasites of parasites that rely on the bacterial host and another helper prophage for survival. We analysed |1,300 genomes of 70 different Streptococcus species for evidence of prophages and identified nearly 800 prophages and satellite prophages, the majority of which are reported here for the first time. We show that prophages and satellite prophages were widely distributed among streptococci, were two clearly different entities and each possessed a structured population. There was convincing evidence that cross-species transmission of prophages is not uncommon. Furthermore, Streptococcus pneumoniae (pneumococcus) is a leading human pathogen worldwide, but the genetic basis for its pathogenicity and virulence is not yet
This HMM represents the phage recombination protein Bet from a number of phage, including phage lambda. All members of this family are found in phage genomes or in putative prophage regions of bacterial genomes ...
Team:Kyoto/Project - 2010.igem.org
In constructing a simple circuit consisting of an inducible promoter and a killer-gene, the combinations are limited by the promoters leak, strength of killer-function and many other factors. We looked for the solution to this problem and came up with an idea of using an anti-killer gene as well with two promoters (a constitutive one and an inducible one). With this circuit, we can apply more promoters to this device by using an appropriate constitutive promoter. We considered that lysis cassette of λ phage and its antagonist, SΔTMD1 are the most appropriate to Killer-gene and Anti-killer gene . This was because some iGEM teams in the past already dealt with lysis cassette and also because we found some articles that shows the effect of SΔTMD1 in the yeast . [learn more] ...
Team:Kyoto/Project - 2010.igem.org
In constructing a simple circuit consisting of an inducible promoter and a killer-gene, the combinations are limited by the promoters leak, strength of killer-function and many other factors. We looked for the solution to this problem and came up with an idea of using an anti-killer gene as well with two promoters (a constitutive one and an inducible one). With this circuit, we can apply more promoters to this device by using an appropriate constitutive promoter. We considered that Lysis cassette of λ phage and its antagonist, SΔTMD1 are the most appropriate to Killer-gene and Anti-killer gene . This was because some iGEM teams in the past already dealt with lysis cassette and also because we found some articles that shows the effect of SΔTMD1 in the yeast . [learn more] ^Top ...
DI-fusion Diversity of accessory genome of human and...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
What I did for the last year....
My major question of interest was, How does molecular stochasticity in the individual cell affect major life history traits? To address this question, I used the enterobacteriophage lambda strain cI857 as a model. Under normal circumstances, cI857 integrates itself into E. colis genome where it is passed horizontally to daughter cells. Most of the phages genome is repressed at this point. However, after a temperature spike, the phage is induced into the lytic cycle. Here the late genes are expressed, including the lysis cassette and the genes that make phage babies. ...
Viruses | Free Full-Text | A Novel Inducible Prophage from Burkholderia vietnamiensis G4 Is Widely Distributed across the...
Burkholderia species have environmental, industrial and medical significance, and are important opportunistic pathogens in individuals with cystic fibrosis (CF). Using a combination of existing and newly determined genome sequences, this study investigated prophage carriage across the species B. vietnamiensis, and also isolated spontaneously inducible prophages from a reference strain, G4. Eighty-one B. vietnamiensis genomes were bioinformatically screened for prophages using PHASTER (Phage Search Tool Enhanced Release) and prophage regions were found to comprise up to 3.4% of total genetic material. Overall, 115 intact prophages were identified and there was evidence of polylysogeny in 32 strains. A novel, inducible Mu-like phage (vB_BvM-G4P1) was isolated from B. vietnamiensis G4 that had lytic activity against strains of five Burkholderia species prevalent in CF infections, including the Boston epidemic B. dolosa strain SLC6. The cognate prophage to vB_BvM-G4P1 was identified in the lysogen genome
Replication of plasmids derived from Shiga toxin-converting bacteriophages in starved Escherichia coli
Podana liczba cytowań wynika z analizy informacji dostępnych w Internecie i jest zbliżona do wartości obliczanej przy pomocy systemu Publish or Perish. ...
Sequence variability of Campylobacter temperate bacteriophages | BMC Microbiology | Full Text
Prophages integrated within the chromosomes of Campylobacter jejuni isolates have been demonstrated very recently. Prior work with Campylobacter temperate bacteriophages, as well as evidence from prophages in other enteric bacteria, suggests these prophages might have a role in the biology and virulence of the organism. However, very little is known about the genetic variability of Campylobacter prophages which, if present, could lead to differential phenotypes in isolates carrying the phages versus those that do not. As a first step in the characterization of C. jejuni prophages, we investigated the distribution of prophage DNA within a C. jejuni population assessed the DNA and protein sequence variability within a subset of the putative prophages found. Southern blotting of C. jejuni DNA using probes from genes within the three putative prophages of the C. jejuni sequenced strain RM 1221 demonstrated the presence of at least one prophage gene in a large proportion (27/35) of isolates tested. Of these,
Beata Nadratowska-Wesolowska - Institutt for medisinske basalfag
Nadratowska-Wesolowska, B., Haugsten, EM., Zakrzewska, M., Jakimowicz, P., Zhen, J., Pajdzik, D., Wesche, J., Wiedlocha, A. (2013) RSK2 regulates endocytosis of FGF receptor 1 by phosphorylation on serine 789. Oncogene Oct21.. Zakrzewska, M., Haugsten, EM., Nadratowska-Wesolowska, B., Oppelt, A., Hausott, B., Jin, Y., Otlewski, J., Wesche, J., Wiedlocha, A. (2013) ERK-mediated phosphorylation of fibroblast growth factor receptor 1 on Ser777 inhibits signaling. Science Signaling Vol. 6, Issue 262.. Nejman, B., Nadratowska-Wesolowska, B., Szalewska-Palasz, A., Wegrzyn, A, Wegrzyn, G. (2011) Replication of plasmids derived from Shiga toxin-converting bacteriophages in starved Escherichia coli. Microbiology 157: 220-233.. Nadratowska-Wesolowska, B., Slominska-Wojewodzka, M., Lyzen, R., Wegrzyn, A., Szalewska-Palasz, A., Wegrzyn, G. (2010) Transcription regulation of the Escherichia coli pcnB gene coding for poly(A) polymerase I: roles of ppGpp, DksA and sigma factors. Molecular Genetics and Genomics ...
Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var...
Genetics Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of genetics and genomics. The journal focuses on articles bearing on heredity, biochemistry, and molecular biology, as well as clinical findings.
Evolutionary paths of streptococcal and staphylococcal superantigens | BMC Genomics | Full Text
Bacterial superantigens (SAgs) have been shown to cause the massive activation of host T cells, strongly influencing immunological disorders. To date, nearly 50 bacterial SAgs and related molecules have been described, primarily from Gram-positive bacteria[1-3]. Streptococcus pyogenes (GAS) is one species of bacteria that harbors SAg genes. Analyses of the entire genomes of 13 GAS isolates have shown that each contains two to seven SAg genes (Additional file1), almost all located in the prophage regions of the genome. In contrast, genes encoding the SAgs speG and smez in GAS strains are not located on these mobile genetic elements, although some are surrounded by transposons. Thus, speG and smez in GAS may have been inherited from an ancestor by horizontal gene transfer. Although speJ in M1 GAS is not located on these mobile genetic elements, speJ is not conserved in the genome sequence of other GAS isolates, except for MGAS6180 (data not shown); in some strains, an SAg similar to speC is called ...
Deep sequencing reveals as-yet-undiscovered small RNAs in Escherichia coli | BMC Genomics | Full Text
In Escherichia coli, approximately 100 regulatory small RNAs (sRNAs) have been identified experimentally and many more have been predicted by various methods. To provide a comprehensive overview of sRNAs, we analysed the low-molecular-weight RNAs (| 200 nt) of E. coli with deep sequencing, because the regulatory RNAs in bacteria are usually 50-200 nt in length. We discovered 229 novel candidate sRNAs (≥ 50 nt) with computational or experimental evidence of transcription initiation. Among them, the expression of seven intergenic sRNAs and three cis-antisense sRNAs was detected by northern blot analysis. Interestingly, five novel sRNAs are expressed from prophage regions and we note that these sRNAs have several specific characteristics. Furthermore, we conducted an evolutionary conservation analysis of the candidate sRNAs and summarised the data among closely related bacterial strains. This comprehensive screen for E. coli sRNAs using a deep sequencing approach has shown that many as-yet-undiscovered
The HMM that is the basis for this family describes a small, pleiotropic protein, DksA (DnaK suppressor A), originally named as a multicopy suppressor of temperature sensitivity of dnaKJ mutants. DksA mutants are defective in quorum sensing, virulence, etc. DksA is now understood to bind RNA polymerase directly and modulate its response to small molecules to control the level of transcription of rRNA. Nearly all members of this family are in the Proteobacteria. Whether the closest homologs outside the Proteobacteria function equivalently is unknown. The low value set for the noise cutoff allows identification of possible DksA proteins from outside the proteobacteria. TIGR02419 describes a closely related family of short sequences usually found in prophage regions of proteobacterial genomes or in known phage ...
Lysis genes of the Bacillus subtilis defective prophage PBSX
Krogh, S, Jorgensen, ST, Devine, KM, Lysis genes of the Bacillus subtilis defective prophage PBSX, JOURNAL OF BACTERIOLOGY, 180, 8, 1998, 2110-2117 ...
ASMscience | Prophage Arsenal of Salm
The emergence in the last two decades of new epidemic Salmonella strains with enhanced virulence traits is indicative of the fast pace of the evolutionary process. This chapter reviews the evidence pointing to a central role played by temperate phages in the dissemination of virulence determinants in the Salmonella complex. The lysogenic condition of most Salmonella strains was recognized prior to an understanding of the genetic bases of lysogeny. Early studies also indicated that some genes of certain prophages escape lysogenic repression and express functions that modify the host bacterium. Since phage and chromosomal sequences near the attachment sites of most prophages are conserved, PCR can be used to assess the phage occupancy of these sites. This approach is particularly attractive because the reaction can be designed in such a way as to always give a signal, and the presence or absence of the prophage can be deduced from the size of the amplified fragment. Preliminary analyses confirmed that the
medical microbiology basics
They can be classified as viruses, bacteria, fungi, protozoa, helminths, or arthropods. The three mechanisms of gene transfer are: Different factors have an influence on the virulence (strength of pathogenicity) of bacteria: Endotoxins: Endotoxins are created during the breakdown of parts of the bacterial cell wall (see above) when bacteria die. Clinical Microbiology made Rediculously Simple. Bacteria are prokaryotes, fungi and parasites are eukaryotes, and viruses are not classified as either. Examples include cholera, botulinum, Infectious diseases caused by fungi are called mycoses. In this case, the viruses completely invade the host cell. Phages are composed of a single- or double-stranded head and a tail, which serves to adhere to the host bacterium. New York: Mcgraw-Hill, 2014. In our daily life, human beings are constantly exposed to micro-organisms. During the lytic replication cycle, DNA is transcribed immediately. Prophages are created, which are first replicated together with the ...
Isolate information: id-3929 (4027-06) - Streptococcus pneumoniae isolates
Brueggemann AB, Harrold CL, Rezaei Javan R, van Tonder AJ, McDonnell AJ, Edwards BA (2017). Pneumococcal prophages are diverse, but not without structure or history. Sci Rep 7:42976 ...
Pharmaceutical Microbiology: Are bacteria as individual as people?
We can think of the bacterial genome as having two parts, says Professor Young. The core genome does the basic housekeeping and is much the same in all members of the species, while the accessory genome has packages of genes that are not essential to the operation of the cell, but can be very useful in coping with aspects of the real world ...
Dr. Gary H. Sakai - Stockton, CA - Dentist Reviews & Ratings - RateMDs
Dr. Gary H. Sakai has a 4.6/5 rating from patients. Visit RateMDs for Dr. Gary H. Sakai reviews, contact info, practice history, affiliated hospitals & more.
CiNii Articles - SAKAI T
メイラード反応後期生成物(AGE)の体内動態解析について (第26回 生体膜と薬物の相互作用シンポジウム 講演要旨集) （2004 ...
CiNii Articles - SAKAI Tohru
懸濁気泡塔における気-液接触反応の物質移動抵抗--パラジウム-アルミナ触媒によるα-メチルスチレンの水素化反応〔固体触媒による気-液接触反応に関する研究-6-〕 （1977 ...
Spontaneous induction of cryptic prophages in populations of the model species Corynebacterium glutamicum and Escherichia coli ...
Spontaneous prophage induction in a small subpopulation of cells which takes place in the absence of a known stimulus is an often observed, but poorly understood phenomenon. With the proposed project we aim to investigate the impact of stress responses and stochasticity fluctuations of key regulatory proteins on the spontaneous induction of the Corynebacterium glutamicum prophage CGP3 at the single cell level. We aim to combine classical microbiological approaches with stochastic modelling and the design of novel microfluidic devices for single cell studies to contribute to a better understanding of spontaneous prophage induction as a general phenomenon in bacterial populations ...
IJMS | Free Full-Text | Solution NMR Structure of Hypothetical Protein CV 2116 Encoded by a Viral Prophage Element in...
CV_2116 is a small hypothetical protein of 82 amino acids from the Gram-negative coccobacillus Chromobacterium violaceum. A PSI-BLAST search using the CV_2116 sequence as a query identified only one hit (E = 2e−07) corresponding to a hypothetical protein OR16_04617 from Cupriavidus basilensis OR16, which failed to provide insight into the function of CV_2116. The CV_2116 gene was cloned into the p15TvLic expression plasmid, transformed into E. coli, and 13C- and 15N-labeled NMR samples of CV_2116 were overexpressed in E. coli and purified for structure determination using NMR spectroscopy. The resulting high-quality solution NMR structure of CV_2116 revealed a novel α + β fold containing two anti-parallel β -sheets in the N-terminal two-thirds of the protein and one α-helix in the C-terminal third of the protein. CV_2116 does not belong to any known protein sequence family and a Dali search indicated that no similar structures exist in the protein data bank. Although no function of CV_2116 could
ASMscience | Bacteriophages and the B
This chapter focuses on the doublestranded DNA (dsDNA) phages, and especially on the temperate phages. While virulent phages certainly perform transduction and engage in evolutionary sparring with their hosts and so influence their evolution, the chapter focuses mainly on the complex interactions of temperate phages with their hosts. Bacteriophages may thus have contributed to the current compact nature of bacterial genomes. The approximately 100 currently published bacterial genome complete nucleotide sequences, and about 285 prophages are related to known bacteriophages. Of the more than 280 prophages in the currently sequenced bacterial genomes, only a few are known to be fully functional bacteriophages. There are two rather complex types of genetic entity in which this appears to have happened: the phage tail-like bacteriocins and the gene transfer agents. To date, protection from other phages and disease virulence factors are the lysogenic conversion genes that have been discovered and studied in
PLOS Genetics: Run-Off Replication of Host-Adaptability Genes Is Associated with Gene Transfer Agents in the Genome of Mouse...
Author Summary Emerging infectious diseases represent an increasing human health problem with many examples of disease outbreaks caused by transmissions from animals to humans, such as, most recently, the bird flu virus. Genes involved in virulence and antibiotic resistance are often carried by mobile elements like plasmids and viruses, which mediate transfer between cells at an amazing speed. Rodents represent a major carrier of infectious agents, and it is therefore particularly important to study the gene transfer processes in bacteria that use rodents as their natural host reservoir. We have studied the genome of a bacterium that is naturally adapted to mice and identified many more putative host-interaction genes than were observed in previously recognized human pathogens. Furthermore, most of these genes are located in a segment of about 25% of the genome, which was massively amplified and packaged into viral particles. This is the first demonstration of targeted packaging of a portion of the
Characterization of the core and accessory genomes of Pseudomonas aeruginosa using bioinformatic tools Spine and AGEnt. -...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
7 Best Mail Transfer Agents (MTAs) for Linux
In this article, we have looked a slight understanding of how MTAs (Mail Transfer Agents) work and a list of the best and most used MTAs on Linux systems.
LoungeBox | PWR 4S HiC Batteries & Charger
HI-C = ULTRA HIGH PWR!. Our PWR Loungebox has quickly established itself on the international market, not only among anglers and campers. Our batteries have also long since replaced the old top dogs for caravans and industrial applications.. With the new HI-C variant, we now offer the HI Power version of the PWR BOX - all dimensions and connections are identical, but thanks to a significantly stronger SMART BMS, even the most powerful e-motors or other power-intensive devices can now be supplied with more than sufficient power (amps).. ...
Sakai City Hall
Sakai City Hallの所在地、電話番号、地図、団体コード、公式サイトなどの情報。自動車やバイクの手続きの際、印鑑証明や住民票の取得にお役立てください。
Gil Melman is Facilitating Spark Energys Power Surge
Spark Energy has grown exponentially in the last few years, with ten major acquisitions since July 2014. Behind the scenes of these acquisitions, and at the helm of the legal team, is Gil Melman, vice president and general counsel, who … Read More
2000W PWR INVRTR - The Shack
USB port 3 AC outlets 2,000W continuous power Digital-volt/watt-meter mountable Overload indicator Remote-control ready thermostat controlled fan 200A power req
BL R/GLASSES 2.25PWR UN - Sizeat MedshopExpress.Com - Medshopexpress
DISCONTINUED BY MANUFACTURER. FOR YOUR INFORMATION PURPOSES, WE HAVE KEPT THIS ITEM PLACEMENT AS COURTESY TO OUR CUSTOMERSYou may search for a related or...
BL R/GLASSES 1.25PWR HF - Sizeat MedshopExpress.Com - Medshopexpress
DISCONTINUED BY MANUFACTURER. FOR YOUR INFORMATION PURPOSES, WE HAVE KEPT THIS ITEM PLACEMENT AS COURTESY TO OUR CUSTOMERSYou may search for a related or...
Extra-chromosomal DNA sequencing reveals episomal prophages capable of impacting virulence factor expression in Staphylococcus...
Staphylococcus aureus is a major human pathogen with well-characterized bacteriophage contributions to its virulence potential. Recently, we identified plasmidial and episomal prophages in S. aureus strains using an extra-chromosomal DNA (exDNA) isolation and sequencing approach, uncovering the plasmidial phage ϕBU01, which was found to encode important virulence determinants. Here, we expanded our extra-chromosomal sequencing of S. aureus, selecting 15 diverse clinical isolates with known chromosomal sequences for exDNA isolation and next-generation sequencing. We uncovered the presence of additional episomal prophages in 5 of 15 samples, but did not identify any plasmidial prophages. exDNA isolation was found to enrich for circular prophage elements, and qPCR characterization of the strains revealed that such prophage enrichment is detectable only in exDNA samples and would likely be missed in whole-genome DNA preparations (e.g., detection of episomal prophages did not correlate with higher ...
Fic/DOC protein family - Wikipedia
In molecular biology, the Fic/DOC protein family is a family of proteins which includes the Fic (filamentation induced by cAMP) protein and doc (death on curing) protein. The Fic protein is involved in cell division and is suggested to be involved in the synthesis of p-aminobenzoate or folate, indicating that the Fic protein and cAMP are involved in a regulatory mechanism of cell division via folate metabolism. This family contains a central conserved motif HPFXXGNG in most members. The exact molecular function of these proteins is uncertain. P1 lysogens of Escherichia coli carry the prophage as a stable low copy number plasmid. The frequency with which viable cells cured of prophage are produced is about 10(-5) per cell per generation. A significant part of this remarkable stability can be attributed to a plasmid-encoded mechanism that causes death of cells that have lost P1. In other words, the lysogenic cells appear to be addicted to the presence of the prophage. The plasmid withdrawal ...
Some mammalian carcinogens and their metabolites affect the viability of Salmonella typhimurium strains, as indicated by a decrease in colony formation, and also induce prophage. We determined the minimum concentration required for prophage induction and the maximum prophage induction frequency for each carcinogen. The latter value was determined by the ratio of the number of induced phage particles relative to that of spontaneously induced phage particles in the controls. This value is constant for each carcinogen, regardless of its concentration. Since damage of the bacterial genome results in prophage induction, the reactivity of each compound with the genome may be indicated by the minimum concentration required for prophage induction and the maximum frequency of prophage induction. Carcinogens unable to affect bacterial viability are also unable to induce prophage. Failure to induce prophage indicates a requirement for metabolic activation by mammalian enzymes. Interaction of these ...
Protein-Mediated and RNA-Based Origins of Replication of Extrachromosomal Mycobacterial Prophages | mBio
We have described here the putative replication origins and partitioning functions of a series of temperate mycobacteriophages whose prophages are maintained extrachromosomally. Although more than 100 such phages have been reported, only a minority (5%) use a RepA-like initiator protein like that of the prototype P1 prophage. We have demonstrated that RepA is both required and sufficient for autonomous replication, and the cis-acting ori sequences presumably lie within or immediately adjacent to repA. However, most of the autonomously replicating phages do not have repA, there are no identifiable protein-coding genes, and it is likely that they use the transcribed RNA to initiate replication. A region expressing these RNAs is necessary and sufficient for autonomous replication.. Mapping and characterizing these prophage replication origins are confounded by differences in the behaviors of related systems derived from different phages, necessitating inclusion of many different systems in the ...
Browsing by Subject Bacteriophages -- Research
Human infections with Shiga-toxin (Stx)-producing E. coli (STEC) vary in severity of illness. The pan-genome of a bacterial species contains a shared, essential core genome, and a variably distributed accessory genome ...
Tower of Druagas Gil Zaps to Smash! | Smashboards
Gil, depicted as surprised as you are that he finally got his own thread Who is Gil? The average Gil, diagramed Prince Gilgamesh, usually known simply...
Action against RMT Activity Involving Power Leveling :: World of Warcraft :: ZAM
I take from this that if I join a FC pty or forbid go out w/ my LS to farm KIs from gold boxes I may be supporting RMT or assumed to be RMT. Unless I physically buy the gil Im not supporting them. Find the 10 day old character w/ 20 mil gil on him and 50 mil in gear hmmm might need to look into that ya think. Besides why anyone buys gil anymore is beyond me. SE comes in ban hammer blazing and inhibits its player base from making gil in an attempt to keep them from buying gil that we arent buying so that we end up buying gil. **** just remove Fell Cleave as a WS all together ...
i heart photograph: guest blogger • PWR PAPER
march 7-28, 2009 height=236 id=Image94_img src=http://2.bp.blogspot.com/_9oyXNTMdFhw/SbaNFzQKgoI/AAAAAAAAO5Q/w-XGlULVDeM/S1600-R/_laurelptak-inreallife.jpg width=200 ...
Adding pwr fold signal mirrors 08 gmc - Page 4 - PerformanceTrucks.net Forums
Originally Posted by 01WS6/tamu This is for an 08 gmc sle-1 interior. Upgraded to sle-2 with DL3 option. Must change door harness bezels, and swap to
Complete genome sequence of the Phaeobacter gallaeciensis type strain CIP 105210(T) (= DSM 26640(T) = BS107(T)).
Phaeobacter gallaeciensis CIP 105210(T) (= DSM 26640(T) = BS107(T)) is the type strain of the species Phaeobacter gallaeciensis. The genus Phaeobacter belongs to the marine Roseobacter group (Rhodobacteraceae, Alphaproteobacteria). Phaeobacter species are effective colonizers of marine surfaces, including frequent associations with eukaryotes. Strain BS107(T) was isolated from a rearing of the scallop Pecten maximus. Here we describe the features of this organism, together with the complete genome sequence, comprising eight circular replicons with a total of 4,448 genes. In addition to a high number of extrachromosomal replicons, the genome contains six genomic island and three putative prophage regions, as well as a hybrid between a plasmid and a circular phage. Phylogenomic analyses confirm previous results, which indicated that the originally reported P. gallaeciensis type-strain deposit DSM 17395 belongs to P. inhibens and that CIP 105210(T) (= DSM 26640(T)) is the sole genome-sequenced ...
Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX...
Vibrio cholerae O1 isolates belonging to the Ogawa serotype, El Tor biotype, harbouring the classical CTX prophage were first isolated in Mozambique in 2004. Multilocus sequence typing (MLST) analysis using nine genetic loci showed that the Mozambique isolates have the same sequence type (ST) as O1 …
The dissemination of C10 cysteine protease genes in Bacteroides fragilis by mobile genetic elements | BMC Microbiology | Full...
The C10 family of cysteine proteases includes enzymes that contribute to the virulence of bacterial pathogens, such as SpeB in Streptococcus pyogenes. The presence of homologues of cysteine protease genes in human commensal organisms has not been examined. Bacteroides fragilis is a member of the dominant Bacteroidetes phylum of the human intestinal microbiota, and is a significant opportunistic pathogen. Four homologues of the streptococcal virulence factor SpeB were identified in the B. fragilis genome. These four protease genes, two were directly contiguous to open reading frames predicted to encode staphostatin-like inhibitors, with which the protease genes were co-transcribed. Two of these protease genes are unique to B. fragilis 638R and are associated with two large genomic insertions. Gene annotation indicated that one of these insertions was a conjugative Tn-like element and the other was a prophage-like element, which was shown to be capable of excision. Homologues of the B. fragilis C10
Genome sequence of temperate bacteriophage Psymv2 from Antarctic dry valley soil isolate Psychrobacter sp. MV2
A temperate phage, Psymv2, was isolated from an Antarctic soil bacterium, Psychrobacter sp. MV2. The morphology of Psymv2 was typical of the Siphoviridae, with an isometric head and non-contractile tail. The Psymv2 genome was found to be 35,725 bp in length, had a G + C content of 44.5 %, with 49 protein-coding genes and one tRNA gene predicted. Integration of Psymv2 occurred at an ssrA gene, with the last 27 bases of this gene directly repeated at the prophage ends. The genome was organised in a modular fashion: integration, regulation, packaging, head assembly, tail assembly, host specificity and lysis. While the genome sequence had little similarity on a nucleotide level to previously reported phage sequences, the genome architecture resembled that of Siphoviridae of low G + C Gram-positive bacteria. The closest relatives to Psymv2 were uncharacterized putative prophages within the P. arcticus 273-4 and Acinetobacter baumannii 6013113 genomes. Global alignment of the Psymv2 genome and these ...
Complete genome sequence of a marine roseophage provides evidence into the evolution of gene transfer agents in...
Roseophage RDJLΦ1 is a siphovirus isolated from South China Sea on Roseobacter denitrificans OCh114. Its virion encapsulates 62.7 kb genome that encodes 87 gene products. RDJLΦ1 shares similar genome organization and gene content with the marine bacteriophage ΦJL001 and Pseudomonas phages YuA and M6, which are different from those of typical λ- or Mu-like phages. Four hallmark genes (ORFs 81 to 84) of RDJLΦ1 were highly homologous to RcGTA-like genes 12 to 15. The largest gene (ORF 84) was predicted to encode a tail fibre protein that could be involved in host recognition. Extended phylogenetic and comparative genomic analyses based on 77 RcGTA-like element-containing bacterial genomes revealed that RcGTA-like genes 12 to 15 together appear to be a conserved modular element that could also be found in some phage or prophage genomes. Our study suggests that RcGTA-like genes-containing phages and prophages and complete RcGTAs possibly descended from a same prophage ancestor that had diverged and then
Difference between revisions of Lactobacillus chromosomal integration - OpenWetWare
This procedure is used to integrate a desired DNA cassette into the chromosome of Lactobacillus plantarum at specific locations. This protocol can also be modified to perform gene knockouts and other chrosomal modifications. The two plasmids used for this procedure are pGIP73 and pP7B6 which integrate into the conjugated bile-acid hydrolase (cbh) sequence and the P7B6 prophage sequence respectively. These plasmids are non-replicative in Lactobacillus spp. and operate based on homologous recombination between the plasmid and the chromosome. The desired cassette is inserted in a unique XbaI site in the middle of both the CBH and P7B6 sequences. ...
Frontiers | In silico Evolution of Lysis-Lysogeny Strategies Reproduces Observed Lysogeny Propensities in Temperate...
Bacteriophages are the most abundant organisms on the planet and both lytic and temperate phages play key roles as shapers of ecosystems and drivers of bacterial evolution. Temperate phages can choose between (i)lysis: exploiting their bacterial hosts by producing multiple phage particles and releasing them by lysing the host cell, and (ii) lysogeny: establishing a potentially mutually beneficial relationship with the host by integrating their chromosome into the host cells genome. Temperate phages exhibit lysogeny propensities in the curiously narrow range of 5-15%. For some temperate phages, the propensity is further regulated by the multiplicity of infection, such that single infections go predominantly lytic while multiple infections go predominantly lysogenic. We ask whether these observations can be explained by selection pressures in environments where multiple phage variants compete for the same host. Our models of pairwise competition, between phage variants that differ only in their
What does lysogeny mean?
Definition of lysogeny in the Definitions.net dictionary. Meaning of lysogeny. What does lysogeny mean? Information and translations of lysogeny in the most comprehensive dictionary definitions resource on the web.
GTA V Minimap to GTA IV - GTA IV - GTAForums
Page 1 of 9 - GTA V Minimap to GTA IV - posted in GTA IV: Im currently trying to make a GTA V HUD for GTA IV. I have made the border rectangle just like GTA V but now I have no idea how to change the health and armour bars into the linear ones shown in GTA V. I also need the radar map to fit in the rectangle also. Wondering whether I would have to make my own script, or if I can just rewrite what they have in the game. There must be something there to say: Hey! The game wan...
PWR fuel for German reactors - Nuclear Engineering International
Germanys RWE Power has ordered Westinghouse fuel and services for the Emsland PWR in Lingen and for the Biblis PWR in Hessen.Emsland uses 18x18 fuel bundles. The fuel will be supplied from the Västerås facility in Sweden and delivered in November...
Phage-like PBSX XkdS (PD033094) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Variabilidade do domínio KH-2 da proteína do retardo mental do X frágil (FMRP)
A proteína do retardo mental do X frágil (FMRP), codificada pelo gene do Retardo Mental do X Frágil (do inglês, Fragile Mental Retardation 1, FMR1) tem expressão significativa no encéfalo, gônadas e células...
Gil Ozeri - IMDb
Gil Ozeri, Writer: Big Mouth. Gil Ozeri is a writer and actor, known for Big Mouth (2017), Brooklyn Nine-Nine (2013) and Happy Endings (2011).
Head and Neck Imaging Cases, Buch. Osamu Sakai - ReadRate
Head and Neck Imaging Cases von Osamu Sakai und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
AGUIA PBI - Sakai, T.
Tsurumi, Y ; Tamura, K ; Tanaka, Y ; Koide, Y ; Sakai, M ; Yabana, M ; Noda, Y ; Hashimoto, T ; Kihara, M ; Hirawa, N ; Toya, Y ; Kiuchi, Y ; Iwai, M ; Horiuchi, M ; Umemura, ...
GTA V Wishlist Topic - Page 61 - GTA V - GTAForums
Page 61 of 99 - GTA V Wishlist Topic - posted in GTA V: I would like a semi-automatic Sniper Rifle in a DLC, maybe one modeled after the M110 SASS, and it would be called the Marksman Rifle. To be honest, adding all these automatic weapons is getting kind of boring, how about a new weapon for a different category for once.
GTA V High Life status from insider | Product Reviews Net
We know that most of you are patiently waiting for Rockstar to deliver on the seemingly delayed GTA V High Life update, as well as the GTA V 1.13 update release date. We have been …
俠盜獵車手5 GTA5 PC版常見所有問題解決方法
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1961 Stratocaster build | Page 25 | Telecaster Guitar Forum
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Prophages are able to do a multitude of things within their respective bacterial strains. Prophages can increase the virulence ... the prophage is excised from the bacterial chromosome in a process called prophage induction. After induction, viral ... Bacteriophage λ is able to undergo a type of recombinational repair called prophage reactivation. Prophage reactivation can ... Prophage reactivation in the case of phage λ appears to be an accurate recombinational repair process that is mediated by the ...
Prophage Hp1 holin family
The Prophage Hp1 Hol (Hp1Hol) Family (TC# 1.E.46) consists of a single putative holin (TC# 1.E.46.1.1) of 69 amino acyl ... Portal: Biology As of this edit, this article uses content from "1.E.46 The Prophage Hp1 Hol (Hp1Hol) Family", which is ... "1.E.46 The Prophage Hp1 Hol (Hp1Hol) Family". TCDB. Retrieved 2016-03-29. "BLAST: Basic Local Alignment Search Tool". blast. ...
Analysis of bacterial genomes has shown that a substantial amount of microbial DNA consists of prophage sequences and prophage- ... A detailed database mining of these sequences offers insights into the role of prophages in shaping the bacterial genome: ... ISBN 978-1-904455-87-5. Canchaya C, Proux C, Fournous G, Bruttin A, Brüssow H (June 2003). "Prophage genomics". Microbiology ... ISBN 978-1-904455-14-1. Fouts DE (November 2006). "Phage_Finder: automated identification and classification of prophage ...
"Fitness benefits to bacteria of carrying prophages and prophage‐encoded antibiotic‐resistance genes peak in different ... Sometimes prophages may provide benefits to the host bacterium while they are dormant by adding new functions to the bacterial ... Strategies to combat certain bacterial infections by targeting these toxin-encoding prophages have been proposed. Bacterial ... Henrot C, Petit MA (September 2022). "Signals triggering prophage induction in the gut microbiota". Molecular Microbiology. doi ...
... contain P2-like prophages . Of these P2-like prophages is P2 best characterized. The P2 phage was found to be able to multiply ... Since that time, a large number of P2-like prophages (e.g. 186, HP1, HK239, and WΦ) have been isolated that shared characters ... Since the C repressor is not inactivated by the SOS/RecA system of E. coli, the P2 prophage is non-inducible by ultraviolet ... found that the lysogenic E. coli having a λ, P1, P2, or Mu prophage could grow more rapidly than a non-lysogenic counterpart ...
Most genomes comprise prophages wherein genetic modifications do not, in general, affect the host genome propagation. Hence, ... Ramisetty BC, Sudhakari PA (2019). "Bacterial 'Grounded' Prophages: Hotspots for Genetic Renovation and Innovation". Frontiers ... there is higher probability of genetic modifications, in regions such as prophages, which is proportional to the probability of ...
These grounded prophages and other such genetic elements are sites where genes could be acquired through horizontal gene ... Ramisetty BC, Sudhakari PA (2019). "Bacterial 'Grounded' Prophages: Hotspots for Genetic Renovation and Innovation". Frontiers ... prophages (i.e. integrated phage that cannot produce new phage) are buffer zones which would tolerate variations thereby ...
DUF2693 RNA motif
However, this domain is annotated as being associated with prophages. Because phages often organize their genes into long ...
Integrated prophages were found in the genome of A. capsulatum. and full complements of flagellar and chemotaxis genes were ...
It induces lytic development in certain bacteria that contain prophages. Arthur JC (2020). "Microbiota and colorectal cancer: ... "The bacterial toxin colibactin triggers prophage induction". Nature. 603 (7900): 315-320. Bibcode:2022Natur.603..315S. doi: ...
In its inactive form, a prophage gets passed on each time the host cell divides. If prophages become active, they can exit the ... The daughter cells can continue to replicate with the prophage present or the prophage can exit the bacterial chromosome to ... Also, the repressor produced by the prophage that prevents prophage genes from being expressed confers immunity for the host ... which lead to prophage induction. One potential strategy to combat prophage induction is through the use of glutathione, a ...
"The Role of Prophage in Plant-Pathogenic Bacteria". Annual Review of Phytopathology. Annual Reviews. 51 (1): 429-451. doi: ...
Varani AM, Monteiro-Vitorello CB, Nakaya HI, Van Sluys MA (4 August 2013). "The role of prophage in plant-pathogenic bacteria ... Viruses portal Antimicrobial resistance Paul E. Turner Phage display Phage monographs Phagoburn Prophage This article was ... including prophages and plasmids, and thus may spread quite rapidly even without direct selection. Nevertheless, in contrast to ...
... expressed by genes considered to be part of the genome of lambdoid prophages. The toxins are named after Kiyoshi Shiga, who ... "The So-called Chromosomal Verotoxin Genes are Actually Carried by Defective Prophages" (doi:10.1093/dnares/6.2.141) (CS1: long ... "The so-called chromosomal verotoxin genes are actually carried by defective prophages". DNA Research. 6 (2): 141-3. doi:10.1093 ...
The host is termed a lysogen when a prophage is present. This prophage may enter the lytic cycle when the lysogen enters a ... a phenomenon termed prophage reactivation. Prophage reactivation in phage λ appears to occur by a recombinational repair ... The prophage is duplicated with every subsequent cell division of the host. The phage genes expressed in this dormant state ... In this state, the λ DNA is called a prophage and stays resident within the host's genome without apparent harm to the host. ...
A prophage is either integrated into the host bacteria's chromosome or more rarely exists as a stable plasmid within the host ... The prophage expresses gene(s) that repress the phage's lytic action, until this repression is disrupted (see lytic cycle). ... prophages as active regulatory switches of bacteria". Nature Reviews Microbiology. 13 (10): 641-650. doi:10.1038/nrmicro3527. ...
Harms A, Fino C, Sørensen MA, Semsey S, Gerdes K (December 2017). "Prophages and Growth Dynamics Confound Experimental Results ...
SPP1 holin family
Krogh, S.; Jørgensen, S. T.; Devine, K. M. (1998-04-01). "Lysis genes of the Bacillus subtilis defective prophage PBSX". ...
Peptidoglycan binding domain
"Lysis genes of the Bacillus subtilis defective prophage PBSX". J. Bacteriol. 180 (8): 2110-2117. doi:10.1128/JB.180.8.2110- ...
René Thomas (biologist)
Thomas further discovered that some of the genes of the prophage, even though they are negatively regulated by the prophage's ... I. Induction of prophage genes following hetero-immune superinfection". Journal of Molecular Biology. 22: 79-95. doi:10.1016/ ... "prophage") due to the repression of all viral genes by the product of a bacteriophage regulatory gene. In this respect, Thomas ...
D12-methyl RNA motif
However, many of the associated genes are typical of those located in prophages. Since phage genomes often consist of a small ...
In fact, the presence of oil in the environment can induce prophages and the subsequent lysis of a huge number of bacteria. At ... Cochran, P. K.; Kellogg, C. A.; Paul, J. H. (1998-10-23). "Prophage induction of indigenous marine lysogenic bacteria by ... Jiang, SC; Paul, JH (1996). "Occurrence of lysogenic bacteria in marine microbial communities as determined by prophage ...
The gene coding for it is found in a prophage. It bears homology to ORF904 of plasmid pRN1 from Sulfolobus islandicus, which ...
Unlike prophages, proviruses do not excise themselves from the host genome when the host cell is stressed.[page needed] This ... Prophage Phage Retrotransposon Germline Horizontal gene transfer Endogenous retrovirus Endogenous viral element Adeno- ... In the case of bacterial viruses (bacteriophages), proviruses are often referred to as prophages. However, proviruses are ... distinctly different from prophages and these terms should not be used interchangeably. ...
Prophages are genomes of bacteriophages (a type of virus) that are inserted into bacterial chromosomes; prophages can then be ... the most common mobile genetic elements in the prokaryotic genome are plasmids and prophages. Plasmids and prophages can move ... Prophages can loop out of bacterial chromosomes to produce bacteriophages that go on to infect other bacteria with the ... prophages; this allows prophages to propagate quickly among the bacterial population, to the harm of the bacterial host. ...
Gene transfer agent
... s, like defective prophages, arise by mutation of prophages, but they retain functional genes for the head ... Unlike prophage genes, the genes encoding GTAs are not excised from the genome and replicated for packaging in GTA particles. ... Such prophages often acquire mutations that make them defective and unable to produce phage particles. Many bacterial genomes ... Motro Y, La T, Bellgard MI, Dunn DS, Phillips ND, Hampson DJ (March 2009). "Identification of genes associated with prophage- ...
Allan M. Campbell
The dormant form of the lambda genome was called the 'prophage'. Study of phage lambda over the next 50 years provided valuable ...
These sites often contain tandem arrays of integrated CTXφ prophage. In addition to the ctxA and ctxB genes encoding cholera ...
A prophage is a virus that has inserted itself into the genome of the host bacterium. TABLE 1. Bacterial virulence properties ... The toxin gene is encoded by a prophage called corynephage β. The toxin causes the disease in humans by gaining entry into the ...
Danovaro, R.; Corinaldesi, C. (February 1, 2003). "Sunscreen Products Increase Virus Production Through Prophage Induction in ...
"Prophage exotoxins enhance colonization fitness in epidemic scarlet fe" by Stephan Brouwer, Timothy Barnett et al.
... to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir ... These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins. ... to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir ... These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins. ...
Comparative Genome Analysis of Listeria Bacteriophages Reveals Extensive Mosaicism, Programmed Translational Frameshifting, and...
Structural and functional analysis of a prophage - York Research Database
intact or incomplete PROPHAGE
Criteria for scoring prophage regions (as intact, questionable, or incomplete):. Method 1:. 1. If the number of certain phage ... Total : 0 prophage regions have been identified, of which 0 regions are intact, 0 regions are incomplete, 0 regions are ... COMPLETENESS: a prediction of whether the region contains a intact or incomplete prophage based on the above criteria. SCORE: ...
Rise and Persistence of Global M1T1 Clone of Streptococcus pyogenes - Volume 14, Number 10-October 2008 - Emerging Infectious...
Contribution of Prophages to Emergence of Global M1T1 Strain Acquisition of Novel Virulence Genes by Global M1T1 Strain and ... Contribution of Prophages to Emergence of Global M1T1 Strain. In 1996, Cleary et al. found that the globally disseminated M1T1 ... More prophages enrich the bacteria with additional toxins, but they may also bring the potential risk of lysing the bacteria at ... Two virulence genes, speA2 and sda1, were introduced into the M1T1 strain by prophages and are likely to have contributed to ...
Classical RS1 and environmental RS1 elements in Vibrio cholerae O1 El Tor strains harbouring a tandem repeat of CTX prophage:...
Darrell Bayles : USDA ARS
Identification of a Divided Genome for VSH-1, the Prophage-Like Gene Transfer Agent of Brachyspira hyodysenteriae - (Peer ... Identification of a Divided Genome for VSH-1, the Prophage-Like Gene Transfer Agent of Brachyspira hyodysenteriae. Journal of ... Antibiotics in feed induce prophages in swine fecal microbiomes - (Peer Reviewed Journal) ... The agricultural antibiotic carbadox induces prophage and antibiotic resistance gene transfer in multidrug-resistant salmonella ...
Characterization of Mu prophage lacking the central strong gyrase binding site: Localization of the block in replication
Host-prophage interactions in bacterial populations - Leibniz Institute for Natural Product Research and Infection Biology -...
Tight Regulation of the Gene of the KplE1 Prophage... | preLekára.sk
2006 Prophage Finder: a prophage loci prediction tool for prokaryotic genome sequences. In Silico Biol 6 223 227 2006060020 [ ... Tight Regulation of the Gene of the KplE1 Prophage: A New Paradigm for Integrase Gene Regulation English version České info ... In silico analysis revealed that overlap of the attL region with the integrase promoter is widely encountered in prophages ... In silico analysis revealed that overlap of the attL region with the integrase promoter is widely encountered in prophages ...
IMSEAR at SEARO: Effects of prophages HPlcl & a defective prophage carried by Haemophilus influenza Rd on transfection by phage...
Universidade do Minho: Induction and initial characterization of a new Helicobacter pylori prophage
Induction and initial characterization of a new Helicobacter pylori prophage. Author(s): Ferreira, Rute Vanessa Novais. Sousa, ... The prophage forms small uniform plaques (1mm diameter) in five of the 76 H. pylori strains tested. TEM analysis revealed that ... Employing UV radiation for 60 seconds as an inducing agent, we were able to isolate a new H. pylori prophage from the lysate of ... Phages can be reversibly integrated into the host bacterial genome as prophages and, in the case of H. pylori, previous studies ...
Science Clips - Volume 9, Issue 23, June 13, 2017
Effects of Prophage Mu Induction on Expression of Adjacent Host Genes - CSHL Scientific Digital Repository
EU H2020 Project 'PROPHARG (Evolution of Prophages that carry Antibiotic Resistance Genes (ARGs) and..)': description,...
Evolution of Prophages that carry Antibiotic Resistance Genes (ARGs) and their host-bacteria in response to antibiotics and ... prophages antibiotics arg overuse frequency encode incorporate underlying carry encodes abundance compounds resistant vast cell ... prophage sequence args cycle enters serial carriage fitness nature caused benefits induce material assays lyses evolution ... I predict that the net effect of a prophage that encodes an ARG on the growth and evolution of its host bacterium will strongly ...
Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to...
Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. ... Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ... Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ... Anti-Bacterial Agents, Bacterial Drug Resistance, Ciprofloxacin, Fluoroquinolones, Microbial Sensitivity Tests, Prophages, ...
B. Appel | Semantic Scholar
2xx6.1 | SWISS-MODEL Template Library
STRUCTURE OF THE BACILLUS SUBTILIS PROPHAGE DUTPASE, YOSS ... STRUCTURE OF THE BACILLUS SUBTILIS PROPHAGE DUTPASE, YOSS ... SPBC2 PROPHAGE-DERIVED DEOXYURIDINE 5-TRIPHOSPHATE NUCLEOTIDOHYDROLASE YOSS: ABC. SMTL:PDB. SMTL Chain Id:. PDB Chain Id:. A. ... Garcia-Nafria, J. et al., The Structure of Bacillus Subtilis Sp Beta Prophage Dutpase and its Complexes with Two Nucleotides. ...
IUCr) Acta Crystallographica Section D Volume 75, Part 11, November 2019
The lytic cassette proteins XepA and YomS from Bacillus subtilis prophages have been characterized and it was found that only ... Cover illustration: The Bacillus subtilis PBSX prophage lytic cassette protein XepA adopts a dumbbell shaped pentamer. ... Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS. ...
Gerald B. Koudelka - Search Results - PubMed
The Marine Viromes of Four Oceanic Regions | PLOS Biology
Phages affect microbial evolution by inserting themselves into genomes as prophages. Prophages often account for most of the ... The prophage sequences for these analyses were extracted from complete microbial genomes. A complete list is available at the ... An electronic version of the tree and a FASTA list of phage and prophage genomes used to make the tree are available at the ... In addition, the sequences were compared to the phage and prophage sequences from 510 genomes of the phage genome database (RA ...
British Library EThOS: An environmental study of the factors contributing to the control of Arthrospira spp. in East African...
Frontiers | More Is Better: Selecting for Broad Host Range Bacteriophages
Fortier, L. C., and Sekulovic, O. (2013). Importance of prophages to evolution and virulence of bacterial pathogens. Virulence ... Lysogenic conversion, the expression of phage genes from the prophage, plays a role in pathogenesis of several bacterial ... Labrie, S. J., and Moineau, S. (2007). Abortive infection mechanisms and prophage sequences significantly influence the genetic ... presence of related prophages or particular plasmids (especially for phages adsorbing to pili) and bacterial phage-resistance ...
The biofilm matrix: multitasking in a shared space | Nature Reviews Microbiology
Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long...
Host-prophage association and CRISPR array identification. Longer reads have the potential to provide direct sequence-level ... Phage_Finder: automated identification and classification of prophage regions in complete bacterial genome sequences. Nucleic ... and that long reads can be suitable for identifying the host specificity of assembled viruses/prophages in a metagenomic ... To identify candidate host specificity for assembled prophage genomes, we used a heuristic alignment strategy with our error- ...
Agenus Says Phase 2 Study Of Brain Cancer Vaccine Shows Improved Survival
Monday said a Phase 2 study result showed that more than 90 percent of aggressive brain cancer patients treated with Prophage ... Prophage Series vaccines are individualized cancer vaccines. Each Prophage Series vaccine is manufactured using a patients own ... Agenus said the next phase of development is underway with an NCI funded, large-scale, randomized trial investigating Prophage ... Prophage Series vaccines are currently being studied in both newly diagnosed and recurrent GBM. ...
Diabetic Foot Infections Guidelines: Guidelines Summary
Did God Make Pathogenic Viruses? | Answers in Genesis
An important category of small replicons are the prophages, phages and other virus types. Further, no bacterium has yet been ... The incorporation of plasmids or prophages into the bacterial chromosomes is called transfection. The incorporation of only ... In nearly all prokaryotes, it occurs commonly through the intermediary of an infectious form of prophage, the temperate ... In this state a phage genome is referred to as a prophage. ... pathogenic because of virulence genes brought by a prophage or ...
- Consequently, the att L recombination region overlaps with the integrase promoter, and the integrase and RDF genes do not share a common activated promoter upon lytic induction as in the lambda prophage. (prelekara.sk)
- In silico analysis revealed that overlap of the att L region with the integrase promoter is widely encountered in prophages present in prokaryotic genomes, suggesting a general occurrence of negatively autoregulated integrase genes. (prelekara.sk)
- Here, we screened the presence of prophages genes (integrases and holin) in a set of 73 H. pylori Portuguese clinical strains by PCR assays. (uminho.pt)
- EU H2020 Project 'PROPHARG (Evolution of Prophages that carry Antibiotic Resistance Genes (ARGs) and. (fabiodisconzi.com)
- An important mechanism of transferring antibiotic resistance genes (ARGs) among bacteria are temperate bacteriophages (prophages), viruses that can incorporate their own genetic material into the bacterial chromosome, thereby providing their bacterial host (which is now called a 'lysogen') with additional genes, such as ARGs. (fabiodisconzi.com)
- For example, many nonpathogens and pathogens only differ by prophages that encode exotoxin genes [ 5 ]. (plos.org)
- Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage. (emdmillipore.com)
- Phages can be reversibly integrated into the host bacterial genome as prophages and, in the case of H. pylori, previous studies have identified the integrase gene in about 20 % of the genomes. (uminho.pt)
- The genomes of positive strains were sequenced and prophages content was analysed using the PHASTER tool. (uminho.pt)
- 3) I will sequence the evolved lysogens including their prophage genomes to detect underlying genomic changes associated with bacterial adaptation to prophage carriage. (fabiodisconzi.com)
- Phages affect microbial evolution by inserting themselves into genomes as prophages. (plos.org)
- For example, bacteria can become infected with viruses that integrate into their genomes, becoming genetic elements called prophages. (uofmhealth.org)
- 2008 Host responses influence on the induction of lambda prophage. (prelekara.sk)
- Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. (mdc-berlin.de)
- Together, our findings show that ciprofloxacin treatment of S. maltophilia leads to the secretion of a heterogeneous pool of MVs and the induction of prophages that are potentially involved in adverse side-effects during antibiotic treatment. (mdc-berlin.de)
- Molecular Mechanisms Governing "Hair-Trigger" Induction of Shiga Toxin-Encoding Prophages. (nih.gov)
- Many of these bacteria are infected with predicted prophages, and we show that the expression of their ClbS homologs provides immunity from colibactin-triggered induction. (biorxiv.org)
- Specifically, using constructed lysogens (carrying prophage lambdaARG) of E. coli bacteria, I will (1) use competitive fitness assays to determine the costs/benefits for bacteria of carrying prophages that encode AR and how these costs depend on environmental antibiotic concentrations and the frequency with which the prophage enters the lytic cycle (i.e. the prophage becomes active, replicates and lyses the host cell). (fabiodisconzi.com)
- I predict that the net effect of a prophage that encodes an ARG on the growth and evolution of its host bacterium will strongly depend on both the frequency with which the phage enters the lytic cycle and the costs/benefits of the ARG. (fabiodisconzi.com)
- The Bacillus subtilis PBSX prophage lytic cassette protein XepA adopts a dumbbell shaped pentamer. (iucr.org)
- Here, we discover that colibactin targets bacteria carrying prophages, inducing lytic development via the bacterial SOS response. (biorxiv.org)
- For most, passive replication of the phage genome relies on integration into the host's chromosome and becoming a prophage. (prelekara.sk)
- Prophages remain silent in the absence of stress and replicate passively within their host genome. (prelekara.sk)
- Phage infection of bacteria can lead to lysis of the host, or to a state in which the phage genome has integrated into bacterial DNA as a prophage. (virology.ws)
- Secondly, a method we call IdentiPhage was established describing the prediction of integrated prophages in bacterial genome hosts. (uni-goettingen.de)
- IMSEAR at SEARO: Effects of prophages HPlcl & a defective prophage carried by Haemophilus influenza Rd on transfection by phage S2 DNA. (who.int)
- 2) I will follow the evolution of these lysogens that carry AR-encoding prophages using a serial transfer experiment in the presence/absence of antibiotics and compounds that induce phage lysis. (fabiodisconzi.com)
- The prophage-inducing effects we observe apply broadly across taxonomically diverse phage-bacteria systems. (biorxiv.org)
- An example is a strain of Salmonella typhimurium that contains a 40 kb prophage (pictured) and is resistant to infection by phage P22. (virology.ws)
- A variety of prophages from different bacteria appear to encode similar phage defense systems with cognate immunity. (virology.ws)
- A phage P22 gene controlling integration of prophage. (microbiologyresearch.org)
- Expert Opinion on Three Phage Therapy Related Topics: Bacterial Phage Resistance, Phage Training and Prophages in Bacterial Production Strains. (dsmz.de)
- Therefore I will study the evolution of ARG-carrying prophages and their host bacteria. (fabiodisconzi.com)
- Deleting the prophage makes the bacteria susceptible to infection by P22. (virology.ws)
- Remove the polyphosphate or the nucleoid-associated protein, said Jakob, and the bacteria suddenly mobilizes these prophages resulting in significant mutations. (uofmhealth.org)
- Joining PCR fragments using λ prophage Red recombination system extracted from bacteria is easier and also cheaper! (hackaday.com)
- Cooperation among Conflict: Prophages Protect Bacteria from Phagocytosis. (bvsalud.org)
- The prophage forms small uniform plaques (1mm diameter) in five of the 76 H. pylori strains tested. (uminho.pt)
- Prophages often account for most of the difference between strains of the same microbial species [ 4 ], and they can dramatically change the phenotype of the hosts via lysogenic conversion. (plos.org)
- Both emm 3 and emm 4 strains in this study possessed 2 prophage-associated superantigens. (cdc.gov)
- In the KplE1 prophage, site-specific recombination is mediated by the IntS integrase and the TorI recombination directionality factor (RDF). (prelekara.sk)
- Tight Regulation of the Gene of the KplE1 Prophage: A New Paradigm for Integrase Gene Regulation. (prelekara.sk)
- The capacity of North-East Asian serotype M12 (emm12) Streptococcus pyogenes (group A Streptococcus, GAS) to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir encoding the secreted superantigens SSA and SpeC and the DNase Spd1. (edu.au)
- A protein called BstA, encoded in the prophage, is essential for defense against superinfection. (virology.ws)
- We predicted and annotated all open reading frames (ORFs) of the prophages by PHASTER and BLASTp. (cdc.gov)
- Cyanophages and a newly discovered clade of single-stranded DNA phages dominated the Sargasso Sea sample, whereas prophage-like sequences were most common in the Arctic. (plos.org)
- We show that IdentiPhagecan locate prophages without any sequence similarities to known phages by testing the method on a set of experimentally identified Inoviridae phages infecting various Vibrio alginolyticus species. (uni-goettingen.de)
- Total : 0 prophage regions have been identified, of which 0 regions are intact, 0 regions are incomplete, 0 regions are questionable. (wishartlab.com)
- Despite their vast abundance in nature, our understanding of the evolution of ARG-carrying prophages is still incomplete. (fabiodisconzi.com)
- Inactivation of prophage in ultraviolet-irradiated Escherichia coli: dependence on recA gene activity. (wikigenes.org)
- She pursued a PhD on the subject of prophage-encoded bacteriophage resistance mechanisms in the dairy bacterium Lactococcus lactis . (ucc.ie)
- Tight Regulation of the Gene of the KplE1 Prophage. (prelekara.sk)
- Examination of the prophage revealed a 63 base pair DNA sequence that is essential for allowing the prophage to reproduce in the presence of BstA. (virology.ws)
- These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins. (edu.au)
- Glioblastoma tumors are often resistant to standard therapies and the extended survival observed in patients treated with Prophage Series vaccine is very promising," said Andrew Parsa, corresponding author of the study and chair of neurological surgery at Northwestern Memorial Hospital and Michael Marchese Professor and chair of the department of neurological surgery at the Feinberg School of Medicine at Northwestern University. (rttnews.com)
- subtilis,'' they differ in the amount and location of prophages, transposable elements, extracellular enzymes, and secondary metabolic pathway operons. (kenyon.edu)
- Employing UV radiation for 60 seconds as an inducing agent, we were able to isolate a new H. pylori prophage from the lysate of clinical strain 11057, using another H. pylori (11507) as the indicator host. (uminho.pt)
- Biotechnology company Agenus Inc. ( AGEN ) Monday said a Phase 2 study result showed that more than 90 percent of aggressive brain cancer patients treated with Prophage Series G-200 were alive at six months after surgery and 30 percent were alive at twelve months. (rttnews.com)
- Under certain conditions, these prophages can lead to the death of the bacterial cell. (uofmhealth.org)
- The prophages can be duplicated for many cell generations until some stimulus induces its activation and virulence. (bvsalud.org)
- However, when stressful conditions occur, a prophage excises itself and resumes the viral cycle. (prelekara.sk)
- By using a novel approach that has been neglected so far (evolution of ARG-carrying prophages) this project will improve our understanding of AR evolution. (fabiodisconzi.com)