Lysogeny
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Attachment Sites, Microbiological
Virus Activation
The mechanism by which latent viruses, such as genetically transmitted tumor viruses (PROVIRUSES) or PROPHAGES of lysogenic bacteria, are induced to replicate and then released as infectious viruses. It may be effected by various endogenous and exogenous stimuli, including B-cell LIPOPOLYSACCHARIDES, glucocorticoid hormones, halogenated pyrimidines, IONIZING RADIATION, ultraviolet light, and superinfecting viruses.
Siphoviridae
Bacteriophage lambda
Salmonella Phages
Chromosomes, Bacterial
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Bacteriophage mu
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Bacteriophage P2
Bacteriolysis
Transduction, Genetic
Recombination, Genetic
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Myoviridae
Mitomycin
SOS Response (Genetics)
An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive.
Sequence Analysis, DNA
Shiga Toxin
Mitomycins
Plasmids
Base Sequence
Virus Integration
Mutation
Bacillus Phages
Defective Viruses
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Escherichia coli O157
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
Genetics, Microbial
Chromosome Mapping
Inovirus
Bacteriophage P1
Shiga Toxin 2
Open Reading Frames
Vibrio cholerae O1
Gene Transfer, Horizontal
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
Interspersed Repetitive Sequences
Copies of transposable elements interspersed throughout the genome, some of which are still active and often referred to as "jumping genes". There are two classes of interspersed repetitive elements. Class I elements (or RETROELEMENTS - such as retrotransposons, retroviruses, LONG INTERSPERSED NUCLEOTIDE ELEMENTS and SHORT INTERSPERSED NUCLEOTIDE ELEMENTS) transpose via reverse transcription of an RNA intermediate. Class II elements (or DNA TRANSPOSABLE ELEMENTS - such as transposons, Tn elements, insertion sequence elements and mobile gene cassettes of bacterial integrons) transpose directly from one site in the DNA to another.
Pseudomonas Phages
Genomic Islands
Distinct units in some bacterial, bacteriophage or plasmid GENOMES that are types of MOBILE GENETIC ELEMENTS. Encoded in them are a variety of fitness conferring genes, such as VIRULENCE FACTORS (in "pathogenicity islands or islets"), ANTIBIOTIC RESISTANCE genes, or genes required for SYMBIOSIS (in "symbiosis islands or islets"). They range in size from 10 - 500 kilobases, and their GC CONTENT and CODON usage differ from the rest of the genome. They typically contain an INTEGRASE gene, although in some cases this gene has been deleted resulting in "anchored genomic islands".
Ultraviolet Rays
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Proviruses
Integrases
Virus Replication
Microscopy, Electron, Transmission
Virulence
Salmonella typhimurium
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Rec A Recombinases
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
Genes
Viral Regulatory and Accessory Proteins
A broad category of viral proteins that play indirect roles in the biological processes and activities of viruses. Included here are proteins that either regulate the expression of viral genes or are involved in modifying host cell functions. Many of the proteins in this category serve multiple functions.
Lactococcus lactis
Corynebacterium diphtheriae
A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.
Roseobacter
Streptococcus pyogenes
Virulence Factors
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
N-Acetylmuramoyl-L-alanine Amidase
Tectiviridae
Gene Expression Regulation, Bacterial
Escherichia coli K12
Operon
Transformation, Genetic
Repressor Proteins
Klebsiella oxytoca
Extrachromosomal Inheritance
Radiation Effects
Integration Host Factors
Cholera Toxin
An ENTEROTOXIN from VIBRIO CHOLERAE. It consists of two major protomers, the heavy (H) or A subunit and the B protomer which consists of 5 light (L) or B subunits. The catalytic A subunit is proteolytically cleaved into fragments A1 and A2. The A1 fragment is a MONO(ADP-RIBOSE) TRANSFERASE. The B protomer binds cholera toxin to intestinal epithelial cells, and facilitates the uptake of the A1 fragment. The A1 catalyzed transfer of ADP-RIBOSE to the alpha subunits of heterotrimeric G PROTEINS activates the production of CYCLIC AMP. Increased levels of cyclic AMP are thought to modulate release of fluid and electrolytes from intestinal crypt cells.
F Factor
Amino Acid Sequence
Conjugation, Genetic
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Gene Expression Regulation, Viral
Salmonella paratyphi C
Genetic Complementation Test
Evolution, Molecular
Shiga-Toxigenic Escherichia coli
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Virion
Haemophilus influenzae
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
DNA Nucleotidyltransferases
Carbadox
Salmonella enterica
Chloramphenicol
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Cholera
Operator Regions, Genetic
Wolbachia
Enterohemorrhagic Escherichia coli
Mozambique
Cloning, Molecular
Norfloxacin
Host Specificity
Synteny
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Chloramphenicol Resistance
Sequence Homology, Nucleic Acid
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Genes, Regulator
Lactobacillus
Mycobacteriophages
RNA, Bacterial
Shiga Toxins
A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.
RNA, Transfer, Arg
Restriction Mapping
Lactobacillus casei
Serratia
DNA, Circular
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
Helper Viruses
Bacteriophage Typing
Sulfamethazine
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Serotyping
Diphtheria Toxin
An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.
Temperature
Inverted Repeat Sequences
Copies of nucleic acid sequence that are arranged in opposing orientation. They may lie adjacent to each other (tandem) or be separated by some sequence that is not part of the repeat (hyphenated). They may be true palindromic repeats, i.e. read the same backwards as forward, or complementary which reads as the base complement in the opposite orientation. Complementary inverted repeats have the potential to form hairpin loop or stem-loop structures which results in cruciform structures (such as CRUCIFORM DNA) when the complementary inverted repeats occur in double stranded regions.
Transformation, Bacterial
R Factors
Centrifugation, Density Gradient
Radiation Genetics
Shigella
Listeria
Lactococcus
Replicon
Annonaceae
Nalidixic Acid
Promoter Regions, Genetic
Blotting, Southern
Bacterial Toxins
Bacterial Typing Techniques
Transcription, Genetic
Shiga Toxin 1
Sequence Homology
Viral Tail Proteins
Electrophoresis, Gel, Pulsed-Field
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
Genotype
Phenotype
Corynebacterium glutamicum
Bacillus
Drug Resistance, Microbial
Exodeoxyribonucleases
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Viral Interference
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
DNA Fingerprinting
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Bacteriophage P22
Exodeoxyribonuclease V
Endopeptidases
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Ribotyping
Mutagenesis, Insertional
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Factor For Inversion Stimulation Protein
A highly abundant DNA binding protein whose expression is strongly correlated with the growth phase of bacteria. The protein plays a role in regulating DNA topology and activation of RIBOSOMAL RNA transcription. It was originally identified as a factor required for inversion stimulation by the Hin recombinase of SALMONELLA and Gin site-specific recombinase of BACTERIOPHAGE MU.
Staphylococcus aureus
Sequence Homology, Amino Acid
DNA Repair
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
DNA, Intergenic
Suppression, Genetic
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
Host-Pathogen Interactions
Kanamycin
RNA, Transfer, Amino Acid-Specific
Deoxyribonucleases, Type II Site-Specific
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4.
Water Microbiology
Drug Resistance, Bacterial
Artificial Gene Fusion
Bacterial Outer Membrane Proteins
Oligonucleotide Array Sequence Analysis
Site-Specific DNA-Methyltransferase (Adenine-Specific)
An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 2.1.1.72.
Inhibition of spontaneous induction of lambdoid prophages in Escherichia coli cultures: simple procedures with possible biotechnological applications. (1/408)
BACKGROUND: Infections of bacterial cultures by bacteriophages are serious problems in biotechnological laboratories. Apart from such infections, prophage induction in the host cells may also be dangerous. Escherichia coli is a commonly used host in biotechnological production, and many laboratory strains of this bacterium harbour lambdoid prophages. These prophages may be induced under certain conditions leading to phage lytic development. This is fatal for further cultivations as relatively low, though still significant, numbers of phages may be overlooked. Thus, subsequent cultures of non-lysogenic strains may be infected and destroyed by such phage. RESULTS: Here we report that slow growth of bacteria decreases deleterious effects of spontaneous lambdoid prophage induction. Moreover, replacement of glucose with glycerol in a medium stimulates lysogenic development of the phage after infection of E. coli cells. A plasmid was constructed overexpressing the phage 434 cI gene, coding for the repressor of phage promoters which are necessary for lytic development. Overproduction of the cI repressor abolished spontaneous induction of the lambda(imm434) prophage. CONCLUSIONS: Simple procedures that alleviate problems with spontaneous induction of lambdoid prophage and subsequent infection of E. coli strains by these phages are described. Low bacterial growth rate, replacement of glucose with glycerol in a medium and overproduction of the cI repressor minimise the risk of prophage induction during cultivation of lysogenic bacteria and subsequent infection of other bacterial strains. (+info)Pilot study of the genetic diversity of the pneumococcal nasopharyngeal flora among children attending day care centers. (2/408)
A pilot study was conducted to determine the genetic diversity of multiple colonies of pneumococci recovered from 37 nasopharyngeal (NP) samples of children. A total of 239 pneumococcal isolates (typically, six to eight colonies per sample) were typed by pulsed-field gel electrophoresis (PFGE). In most NP samples (89%) the multiple colonies shared common PFGE types and serotypes. However, four samples were heterogeneous (samples A through D): each contained two strains with different PFGE types, antibiotypes, and serotypes. Samples A and B each contained one strain of a vaccine capsular type and another expressing a non-vaccine type (according to the currently licensed seven-valent conjugate vaccine). In samples B and C the penicillin MIC for one strain was elevated and the other strain was susceptible. In each of the heterogeneous samples, one of the strains was a representative of an internationally disseminated clone. Samples A, C, and D contained strains which carried prophages that were inducible by mitomycin C and that could be visualized by electron microscopy. The comC gene allele (which encodes the competence-stimulating peptide) was the same in both strains found in each of samples A, B, and D. Carriage of multiple pneumococci with distinct properties should favor genetic exchange and provide a dynamic population structure for pneumococci in their ecological reservoir. Quantitative resolution of majority and minority components of the pneumococcal NP flora will be of importance for evaluation of the impact of intervention strategies such as vaccination or introduction of new antimicrobial agents. (+info)Phenotypes of lexA mutations in Salmonella enterica: evidence for a lethal lexA null phenotype due to the Fels-2 prophage. (3/408)
The LexA protein of Escherichia coli represses the damage-inducible SOS regulon, which includes genes for repair of DNA. Surprisingly, lexA null mutations in Salmonella enterica are lethal even with a sulA mutation, which corrects lexA lethality in E. coli. Nine suppressors of lethality isolated in a sulA mutant of S. enterica had lost the Fels-2 prophage, and seven of these (which grew better) had also lost the Gifsy-1 and Gifsy-2 prophages. All three phage genomes included a homologue of the tum gene of coliphage 186, which encodes a LexA-repressed cI antirepressor. The tum homologue of Fels-2 was responsible for lexA lethality and had a LexA-repressed promoter. This basis of lexA lethality was unexpected because the four prophages of S. enterica LT2 are not strongly UV inducible and do not sensitize strains to UV killing. In S. enterica, lexA(Ind(-)) mutants have the same phenotypes as their E. coli counterparts. Although lexA null mutants express their error-prone DinB polymerase constitutively, they are not mutators in either S. enterica or E. coli. (+info)The Shiga-toxin VT2-encoding bacteriophage varphi297 integrates at a distinct position in the Escherichia coli genome. (4/408)
The plaque-forming VT2-encoding lambdoid bacteriophage varphi297 was isolated from a Belgian clinical Escherichia coli O157:H7 isolate. PCR walking, starting from the int gene of phage varphi297, demonstrated that the varphi297 prophage integrated in the yecE gene of a lysogenic E. coli K12 strain. This integration site, in E. coli K12 and in the original clinical O157:H7 isolate, was confirmed by PCR using primers flanking this site. The excisionase protein of phage varphi297 is identical to the excisionase of VT1-encoding phage VT1-Sakai, while the integrases, which are 82% identical, show significant sequence divergence in the central and C-terminal region. This can explain the different integration sites of both prophages. The activity of the integrase was proven by its ability to mediate the integration of a suicide plasmid, carrying the attachment site of varphi297, at the appropriate position in the E. coli chromosome. (+info)Transcription analysis of Streptococcus thermophilus phages in the lysogenic state. (5/408)
The transcription of prophage genes was studied in two lysogenic Streptococcus thermophilus cells by Northern blot and primer-extension experiments. In the lysogen containing the cos-site phage Sfi21 only two gene regions of the prophage were transcribed. Within the lysogeny module an 1.6-kb-long mRNA started at the promoter of the phage repressor gene and covered also the next two genes, including a superinfection exclusion (sie) gene. A second, quantitatively more prominent 1-kb-long transcript was initiated at the promoter of the sie gene. Another prophage transcript of 1.6-kb length covered a group of genes without database matches that were located between the lysin gene and the right attachment site. The rest of the prophage genome was transcriptionally silent. A very similar transcription pattern was observed for a S. thermophilus lysogen containing the pac-site phage O1205 as a prophage. Prophages from pathogenic streptococci encode virulence genes downstream of the lysin gene. We speculate that temperate phages from lactic streptococci also encode nonessential phage genes ("lysogenic conversion genes") in this region that increase the ecological fitness of the lysogen to further their own evolutionary success. A comparative genome analysis revealed that many temperate phages from low GC content Gram-positive bacteria encode a variable number of genes in that region and none was linked to known phage-related function. Prophages from pathogenic streptococci encode toxin genes in this region. In accordance with theoretical predictions on prophage-host genome interactions a prophage remnant was detected in S. thermophilus that had lost most of the prophage DNA while transcribed prophage genes were spared from the deletion process. (+info)Genome analysis of an inducible prophage and prophage remnants integrated in the Streptococcus pyogenes strain SF370. (6/408)
The mitomycin C inducible prophage SF370.1 from the highly pathogenic M1 serotype Streptococcus pyogenes isolate SF370 showed a 41-kb-long genome whose genetic organization resembled that of SF11-like pac-site Siphoviridae. Its closest relative was prophage NIH1.1 from an M3 serotype S. pyogenes strain, followed by S. pneumoniae phage MM1 and Lactobacillus phage phig1e, Listeria phage A118, and Bacillus phage SPP1 in a gradient of relatedness. Sequence similarity with the previously described prophages SF370.2 and SF370.3 from the same polylysogenic SF370 strain were mainly limited to the tail fiber genes. As in these two other prophages, SF370.1 encoded likely lysogenic conversion genes between the phage lysin and the right attachment site. The genes encoded the pyrogenic exotoxin C of S. pyogenes and a protein sharing sequence similarity with both DNases and mitogenic factors. The screening of the SF370 genome revealed further prophage-like elements. A 13-kb-long phage remnant SF370.4 encoded lysogeny and DNA replication genes. A closely related prophage remnant was identified in S. pyogenes strain Manfredo at a corresponding genome position. The two prophages differed by internal indels and gene replacements. Four phage-like integrases were detected; three were still accompanied by likely repressor genes. All prophage elements were integrated into coding sequences. The phage sequences complemented the coding sequences in all cases. The DNA repair genes mutL and mutS were separated by the prophage remnant SF370.4; prophage SF370.1 and S. pneumoniae phage MM1 integrated into homologous chromosomal locations. The prophage sequences were interpreted with a hypothesis that predicts elements of cooperation and an arms race between phage and host genomes. (+info)Use of real-time quantitative PCR for the analysis of phiLC3 prophage stability in lactococci. (7/408)
Bacteriophages are a common and constant threat to proper milk fermentation. It has become evident that lysogeny is widespread in lactic acid bacteria, and in this work the temperate lactococcal bacteriophage phi LC3 was used as a model to study prophage stability in lactococci. The stability was analyzed in six phi LC3 lysogenic Lactococcus lactis subsp. cremoris host strains when they were growing at 15 and 30 degrees C. In order to perform these analyses, a real-time PCR assay was developed. The stability of the phi LC3 prophage was found to vary with the growth phase of its host L. lactis IMN-C1814, in which the induction rate increased during the exponential growth phase and reached a maximum level when the strain was entering the stationary phase. The maximum spontaneous induction frequency of the phi LC3 prophage varied between 0.32 and 9.1% (28-fold) in the six lysogenic strains. No correlation was observed between growth rates of the host cells and the spontaneous prophage induction frequencies. Furthermore, the level of extrachromosomal phage DNA after induction of the prophage varied between the strains (1.9 to 390%), and the estimated burst sizes varied up to eightfold. These results show that the host cells have a significant impact on the lytic and lysogenic life styles of temperate bacteriophages. The present study shows the power of the real-time PCR technique in the analysis of temperate phage biology and will be useful in work to reveal the impact of temperate phages and lysogenic bacteria in various ecological fields. (+info)Genesis of variants of Vibrio cholerae O1 biotype El Tor: role of the CTXphi array and its position in the genome. (8/408)
The gene encoding cholera toxin, the principal virulence factor of Vibrio cholerae, is encoded by a filamentous, lysogenic bacteriophage known as CTXphi. The genome of V. cholerae, the host for CTXphi, consists of two chromosomes, one large and one small. Here, it is shown that localization and array of CTX prophage DNA in either the large or small chromosome of V. cholerae is likely to be one of the reasons for the emergence of O1 biotype El Tor variants isolated just before and after the V. cholerae O139 cholera outbreak in 1992. Analyses of the organization of the CTX region of the genome of pre-O139 El Tor strains revealed that these strains carry two distinct CTX prophages integrated in the small chromosome in tandem: CTX(ET), the prophage having a conserved NotI site in its repeat sequence segment which seems to be specific for the El Tor strains so far examined, followed by CTX(calc)-like genome, the prophage found in recent O139 clinical isolates from Calcutta. In sharp contrast, in post-O139 El Tor strains only one copy of the CTX(ET) prophage was found to be integrated in the large chromosome. To the authors' knowledge, the presence of CTX prophage in the small chromosome of O1 El Tor strains has not been reported previously. It is also shown that the difference in the CTX copy number and the position of the bacteriophage on the genomes of pre- and post-O139 El Tor strains have an effect on cholera toxin production. While a pre-O139 strain produced maximum cholera toxin in yeast extract/peptone medium at 30 degrees C, a post-O139 El Tor strain showed maximal yield at 37 degrees C, indicating differential regulation of cholera toxin between the strains. It appears from this study that the variation in the integration site of the CTX prophage, its copy number and the presence of diverse phage genomes in V. cholerae O1 biotype El Tor may be strategically important for generating variants with subtle phenotypic modulations of virulence factor production in this longest-ruling seventh pandemic strain. (+info)
Prophages and satellite prophages are widespread among Streptococcus species and may play a role in pneumococcal pathogenesis -...
TIGR01913
Team:Kyoto/Project - 2010.igem.org
Team:Kyoto/Project - 2010.igem.org
DI-fusion Diversity of accessory genome of human and...
What I did for the last year....
Viruses | Free Full-Text | A Novel Inducible Prophage from Burkholderia vietnamiensis G4 Is Widely Distributed across the...
Replication of plasmids derived from Shiga toxin-converting bacteriophages in starved Escherichia coli
Sequence variability of Campylobacter temperate bacteriophages | BMC Microbiology | Full Text
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Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var...
Evolutionary paths of streptococcal and staphylococcal superantigens | BMC Genomics | Full Text
Deep sequencing reveals as-yet-undiscovered small RNAs in Escherichia coli | BMC Genomics | Full Text
TIGR02420
Lysis genes of the Bacillus subtilis defective prophage PBSX
ASMscience | Prophage Arsenal of Salm
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Spontaneous induction of cryptic prophages in populations of the model species Corynebacterium glutamicum and Escherichia coli ...
IJMS | Free Full-Text | Solution NMR Structure of Hypothetical Protein CV 2116 Encoded by a Viral Prophage Element in...
ASMscience | Bacteriophages and the B
PLOS Genetics: Run-Off Replication of Host-Adaptability Genes Is Associated with Gene Transfer Agents in the Genome of Mouse...
Characterization of the core and accessory genomes of Pseudomonas aeruginosa using bioinformatic tools Spine and AGEnt. -...
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Extra-chromosomal DNA sequencing reveals episomal prophages capable of impacting virulence factor expression in Staphylococcus...
Fic/DOC protein family - Wikipedia
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Protein-Mediated and RNA-Based Origins of Replication of Extrachromosomal Mycobacterial Prophages | mBio
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Complete genome sequence of the Phaeobacter gallaeciensis type strain CIP 105210(T) (= DSM 26640(T) = BS107(T)).
Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX...
The dissemination of C10 cysteine protease genes in Bacteroides fragilis by mobile genetic elements | BMC Microbiology | Full...
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Перелік строків подання регулярно оновлюваних звітів з безпеки лікарських засобів
Genomics
Analysis of bacterial genomes has shown that a substantial amount of microbial DNA consists of prophage sequences and prophage- ... A detailed database mining of these sequences offers insights into the role of prophages in shaping the bacterial genome: ... ISBN 978-1-904455-87-5. Canchaya C, Proux C, Fournous G, Bruttin A, Brüssow H (June 2003). "Prophage genomics". Microbiology ... ISBN 978-1-904455-14-1. Fouts DE (November 2006). "Phage_Finder: automated identification and classification of prophage ...
Bacteriophage P2
... contain P2-like prophages . Of these P2-like prophages is P2 best characterized. The P2 phage was found to be able to multiply ... Since that time, a large number of P2-like prophages (e.g. 186, HP1, HK239, and WΦ) have been isolated that shared characters ... Since the C repressor is not inactivated by the SOS/RecA system of E. coli, the P2 prophage is non-inducible by ultraviolet ... found that the lysogenic E. coli having a λ, P1, P2, or Mu prophage could grow more rapidly than a non-lysogenic counterpart ...
Molecular evolution
Most genomes comprise prophages wherein genetic modifications do not, in general, affect the host genome propagation. Hence, ... Ramisetty, Bhaskar Chandra Mohan; Sudhakari, Pavithra Anantharaman (2019). "Bacterial 'Grounded' Prophages: Hotspots for ... there is higher probability of genetic modifications, in regions such as prophages, which is proportional to the probability of ...
Genome evolution
These grounded prophages and other such genetic elements are sites where genes could be acquired through horizontal gene ... However, non-coding regions such as the 'grounded' prophages are buffer zones which would tolerate variations thereby ... Ramisetty BC, Sudhakari PA (2019). "Bacterial 'Grounded' Prophages: Hotspots for Genetic Renovation and Innovation". Frontiers ...
DUF2693 RNA motif
However, this domain is annotated as being associated with prophages. Because phages often organize their genes into long ...
Acidobacterium capsulatum
Integrated prophages were found in the genome of A. capsulatum. and full complements of flagellar and chemotaxis genes were ...
Lysogenic cycle
The daughter cells can continue to replicate with the prophage present or the prophage can exit the bacterial chromosome to ... Also, the repressor produced by the prophage that prevents prophage genes from being expressed confers immunity for the host ... which lead to prophage induction. One potential strategy to combat prophage induction is through the use of glutathione, a ... During induction, prophage DNA is excised from the bacterial genome and is transcribed and translated to make coat proteins for ...
Phage therapy
Viruses portal Antimicrobial resistance Phage display Phage monographs Phagoburn Prophage "Silent Killers: Fantastic Phages?". ... "The Role of Prophage in Plant-Pathogenic Bacteria". Annual Review of Phytopathology. Annual Reviews. 51 (1): 429-451. doi: ...
Shiga toxin
... expressed by genes considered to be part of the genome of lambdoid prophages. The toxins are named after Kiyoshi Shiga, who ... "The so-called chromosomal verotoxin genes are actually carried by defective prophages". DNA Research. 6 (2): 141-3. doi:10.1093 ... "The So-called Chromosomal Verotoxin Genes are Actually Carried by Defective Prophages" (doi:10.1093/dnares/6.2.141). ...
Lambda phage
The host is termed a lysogen when a prophage is present. This prophage may enter the lytic cycle when the lysogen enters a ... a phenomenon termed prophage reactivation. Prophage reactivation in phage λ appears to occur by a recombinational repair ... The prophage is duplicated with every subsequent cell division of the host. The phage genes expressed in this dormant state ... In this state, the λ DNA is called a prophage and stays resident within the host's genome without apparent harm to the host. ...
Lysogen
A prophage is either integrated into the host bacteria's chromosome or more rarely exists as a stable plasmid within the host ... The prophage expresses gene(s) that repress the phage's lytic action, until this repression is disrupted (see lytic cycle). ... prophages as active regulatory switches of bacteria". Nature Reviews Microbiology. 13 (10): 641-650. doi:10.1038/nrmicro3527. ...
Toxin-antitoxin system
Harms A, Fino C, Sørensen MA, Semsey S, Gerdes K (December 2017). "Prophages and Growth Dynamics Confound Experimental Results ...
SPP1 holin family
Krogh, S.; Jørgensen, S. T.; Devine, K. M. (1998-04-01). "Lysis genes of the Bacillus subtilis defective prophage PBSX". ...
Peptidoglycan binding domain
"Lysis genes of the Bacillus subtilis defective prophage PBSX". J. Bacteriol. 180 (8): 2110-2117. doi:10.1128/JB.180.8.2110- ...
René Thomas (biologist)
Thomas further discovered that some of the genes of the prophage, even though they are negatively regulated by the prophage's ... I. Induction of prophage genes following hetero-immune superinfection". Journal of Molecular Biology. 22: 79-95. doi:10.1016/ ... "prophage") due to the repression of all viral genes by the product of a bacteriophage regulatory gene. In this respect, Thomas ...
D12-methyl RNA motif
However, many of the associated genes are typical of those located in prophages. Since phage genomes often consist of a small ...
Primase
The gene coding for it is found in a prophage. It bears homology to ORF904 of plasmid pRN1 from Sulfolobus islandicus, which ...
Streptococcus
18 prophages have been described in S. pneumoniae that range in size from 38 to 41 kb in size, encoding from 42 to 66 genes ...
Provirus
Unlike prophages, proviruses do not excise themselves from the host genome when the host cell is stressed. This state can be a ... However, it is important to note that proviruses are distinctly different from prophages and these terms should not be used ... Prophage Phage Retrotransposon Germline Horizontal gene transfer Endogenous retrovirus Endogenous viral element Adeno- ... In the case of bacterial viruses (bacteriophages), proviruses are often referred to as prophages. ...
Mobilome
Prophages are genomes of bacteriophages (a type of virus) that are inserted into bacterial chromosomes; prophages can then be ... the most common mobile genetic elements in the prokaryotic genome are plasmids and prophages. Plasmids and prophages can move ... Prophages can loop out of bacterial chromosomes to produce bacteriophages that go on to infect other bacteria with the ... prophages; this allows prophages to propagate quickly among the bacterial population, to the harm of the bacterial host. ...
Gene transfer agent
... s, like defective prophages, arise by mutation of prophages, but they retain functional genes for the head ... Unlike prophage genes, the genes encoding GTAs are not excised from the genome and replicated for packaging in GTA particles. ... Such prophages often acquire mutations that make them defective and unable to produce phage particles. Many bacterial genomes ... Motro Y, La T, Bellgard MI, Dunn DS, Phillips ND, Hampson DJ (March 2009). "Identification of genes associated with prophage- ...
Vibrio cholerae
These sites often contain tandem arrays of integrated CTXφ prophage. In addition to the ctxA and ctxB genes encoding cholera ...
Diphtheria toxin
A prophage is a virus that has inserted itself into the genome of the host bacterium. TABLE 1. Bacterial virulence properties ... The toxin gene is encoded by a prophage called corynephage β. The toxin causes the disease in humans by gaining entry into the ...
Sunscreen
Danovaro, R.; Corinaldesi, C. (2003). "Sunscreen Products Increase Virus Production Through Prophage Induction in Marine ...
TM7x
September 2018). "A Linear Plasmid-Like Prophage of Actinomyces odontolyticus Promotes Biofilm Assembly". Applied and ...
Bacteriophage
Sometimes prophages may provide benefits to the host bacterium while they are dormant by adding new functions to the bacterial ... Strategies to combat certain bacterial infections by targeting these toxin-encoding prophages have been proposed. Bacterial ... known as prophages) become active. At this point they initiate the reproductive cycle, resulting in lysis of the host cell. As ... become long-term residents as prophage. Research in 2017 revealed that the bacteriophage Φ3T makes a short viral protein that ...
Scarlet fever
6 October 2020). "Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes". ...
BlyA holin family
2000) provided evidence that BlyAB functions as a prophage-encoded holin system. BlyA promotes endolysin-dependent lysis of an ... a prophage-encoded holin-like system". Journal of Bacteriology. 182 (23): 6791-6797. doi:10.1128/jb.182.23.6791-6797.2000. ISSN ...
Virome
... implications for prophage gene expression". PLOS ONE. 3 (9): e3263. doi:10.1371/journal.pone.0003263. PMC 2533394. PMID ...
Microbiome
Metagenomic analysis of lysogeny in Tampa Bay: implications for prophage gene expression. PLoS One, 3(9), p.e3263. doi:10.1371/ ...
prophages | Journal of Bacteriology
Evidence of Localized Prophage-Host Recombination in the lytA Gene, Encoding the Major Pneumococcal Autolysin María Morales, ... Genomic and Biological Analysis of Phage Xfas53 and Related Prophages of Xylella fastidiosa Elizabeth J. Summer, Christopher J ... Structural Study of the Serratia entomophila Antifeeding Prophage: Three-Dimensional Structure of the Helical Sheath Anindito ... Spontaneous Excision of the Salmonella enterica Serovar Enteritidis-Specific Defective Prophage-Like Element φSE14 Carlos A. ...
Prophage - Wikipedia
Prophages are able to do a multitude of things within their respective bacterial strains. Prophages can increase the virulence ... Bacteriophage λ is able to undergo a type of recombinational repair called prophage reactivation. Prophage reactivation can ... the prophage is excised from the bacterial chromosome in a process called prophage induction. After induction, viral ... Prophage reactivation in the case of phage λ appears to be an accurate recombinational repair process that is mediated by the ...
A Novel P22 Prophage in Salmonella typhimurium | Genetics
A Novel P22 Prophage in Salmonella typhimurium. Diana M. Downs and John R. Roth ... A Novel P22 Prophage in Salmonella typhimurium. Diana M. Downs and John R. Roth ... A Novel P22 Prophage in Salmonella typhimurium. Diana M. Downs and John R. Roth ... The variety of types suggests that the cryptic prophage is mutagenized as a consequence of the induction process. All the ...
Prophage Hp1 holin family - Wikipedia
The Prophage Hp1 Hol (Hp1Hol) Family (TC# 1.E.46) consists of a single putative holin (TC# 1.E.46.1.1) of 69 amino acyl ... Biology portal As of this edit, this article uses content from "1.E.46 The Prophage Hp1 Hol (Hp1Hol) Family", which is licensed ... "1.E.46 The Prophage Hp1 Hol (Hp1Hol) Family". TCDB. Retrieved 2016-03-29. "BLAST: Basic Local Alignment Search Tool". blast. ...
Identification of Prophages and Prophage Remnants within the Genome of Avibacterium paragallinarum Bacterium
Diversity of Prophages in Dominant Staphylococcus aureus Clonal Lineages | Journal of Bacteriology
Identification of S. aureus prophages by multiplex PCR.For a prevalence analysis of S. aureus prophages in a large strain ... We could detect none of the seven prophages in 13% (38/291) of the isolates, one prophage in 36% (106/291), two in 38% (110/219 ... Classification of staphylococcal prophage modules.The identification and description of prophages existing in bacterial strains ... Diversity of Prophages in Dominant Staphylococcus aureus Clonal Lineages Christiane Goerke, Roman Pantucek, Silva Holtfreter, ...
Insights to the diphtheria toxin encoding prophages amongst clinical isolates of Corynebacterium diphtheriae from India. -...
It is imperative to devise prevention strategies that hinder the dissemination of toxin by prophages, as it may increase the ... Insights to the diphtheria toxin encoding prophages amongst clinical isolates of Corynebacterium diphtheriae from India.. ... the present study was undertaken to investigate the prophages integrated into the genome of 29 clinical isolates of C. ...
RCSB PDB - Protein Feature View
- Gifsy-2 prophage protein - H9L447 (H9L447 SALTY)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. - PubMed - NCBI
Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila.. Heymann JB1, Bartho ... The Serratia entomophila antifeeding prophage (Afp) is a bullet-shaped toxin-delivery apparatus similar to the R-pyocins of ... Three-dimensional Structure of the Toxin-delivery Particle Antifeeding Prophage of Serratia entomophila ... Three-dimensional Structure of the Toxin-delivery Particle Antifeeding Prophage of Serratia entomophila ...
Database and Comparative Identification of Prophages | Intelligence | Polsinelli
Viruses | Free Full-Text | Insights into the Functions of a Prophage Recombination Directionality Factor | HTML
RDFs are required to direct the reaction towards excision and to prevent re-integration of the prophage genome when entering a ... are essential players of prophage excisive recombination. Despite the essentially catalytic role of the integrase in both ... Bacterial genomes are parasitized by prophages and prophage remnants, which can constitute up to 20% of the host genome. A pan- ... prophage; lysogeny; recombination directionality factor; integrase; excisionase; response regulator; prophage induction; random ...
Prophage-triggered membrane vesicle formation through peptidoglycan damage in Bacillus subtilis. | Sigma-Aldrich
show that a prophage-encoded endolysin can generate holes in the cell wall through which cytoplasmic membrane material ... Prophage-triggered membrane vesicle formation through peptidoglycan damage in Bacillus subtilis.. [Masanori Toyofuku, Gerardo ... We show that the expression of a prophage-encoded endolysin in a sub-population of cells generates holes in the peptidoglycan ...
yomR - SPbeta prophage-derived uncharacterized protein YomR - Bacillus subtilis (strain 168) - yomR gene & protein
yosG - SPbeta prophage-derived uncharacterized protein YosG - Bacillus subtilis (strain 168) - yosG gene & protein
Viruses | Free Full-Text | Prevalence and Diversity Analysis of Candidate Prophages to Provide An Understanding on Their Roles...
Protein-Mediated and RNA-Based Origins of Replication of Extrachromosomal Mycobacterial Prophages | mBio
This enables the prophage to be passively replicated with the host genome and ensures that a prophage is present in each of the ... 7A). If a given prophage and a particular plasmid are compatible, then we expected to see prophage loss that is no greater than ... Copy numbers of non-RepA prophages.We previously determined that the prophage copy number in a lysogen of the non-RepA phage ... Miko repA is required for prophage replication.To determine whether repA of phage Miko is required for prophage replication, we ...
ASMscience | Lysogeny, Prophage Induc
... it is not necessary that the prophage remains functional as a virus that is capable of prophage induction or lytic growth. ... Foreign genes are most commonly expressed from a prophage in the lysogenic state. This pattern of expression is often termed ... This description will facilitate an understanding of how foreign genes can be expressed during the process of prophage ... Chapter 3 : Lysogeny, Prophage Induction, and Lysogenic Conversion Author: John W. Little1 VIEW AFFILIATIONS HIDE AFFILIATIONS ...
Competition among isolates of Salmonella enterica ssp. enterica serovar Typhimurium: role of prophage/phage in archived...
enterica serovar Typhimurium: role of prophage/phage in archived cultures. Authors. *. Michelle Erickson,. * Cancer Research ... Typhimurium ATCC 14028, phage emerged that had a DNA base sequence segment of prophage ST64B but the sequence differed from the ... 2 in supernatants were identified by primer/PCR as a putative selective force following single plaque isolations on a prophage- ...
Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var...
C. Canchaya, C. Proux, G. Fournous, A. Bruttin, and H. Brüssow, "Prophage genomics," Microbiology and Molecular Biology Reviews ... Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var ... T. Bae, T. Baba, K. Hiramatsu, and O. Schneewind, "Prophages of Staphylococcus aureus Newman and their contribution to ... putative prophages," BMC Microbiology, vol. 6, p. 34, 2006. View at Publisher · View at Google Scholar · View at Scopus ...
The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage
Genome sequence and characteristics of lrm1, a prophage from industrial lactobacillus rhamnosus strain m1 on Environmental XPRT
Prophage Lrm1 was induced with mitomycin C from an industrial Lactobacillus rhamnosus starter culture, M1. Electron microscopy ... a prophage from industrial lactobacillus rhamnosus strain m1. ... Prophage Lrm1 was induced with mitomycin C from an industrial ... Genome sequence and characteristics of lrm1, a prophage from .... Genome sequence and characteristics of lrm1, a prophage from ... resulting in the stability of the defective prophage in its lysogenic state. The presence of a defective prophage in an ...
DNA-Mediated Prophage Induction in Bacillus subtilis Lysogenic for φ105c4 | Journal of Virology
DNA-Mediated Prophage Induction in Bacillus subtilis Lysogenic for φ105c4 Message Subject (Your Name) has forwarded a page to ... DNA-Mediated Prophage Induction in Bacillus subtilis Lysogenic for φ105c4. Anthony J. Garro ... Prophage was induced when strains of Bacillus subtilis 168 lysogenic for φ105c4 were grown to competence and exposed to ... The time course of phage production was similar to that observed for mitomycin C induction of wild-type prophage. Induction was ...
Frontiers | Salmonella enterica Prophage Sequence Profiles Reflect Genome Diversity and Can Be Used for High Discrimination...
Prophage sequences were highly variable among S. enterica serovars with a median ± interquartile range (IQR) of 5 ± 3 prophage ... Prophage sequences were highly variable among S. enterica serovars with a median ± interquartile range (IQR) of 5 ± 3 prophage ... Taken altogether, the diversity of the prophage sequences correlates with genome diversity. Prophage repertoires provide an ... prophage sequences were identified from assembled contigs using PHASTER. We detected 154 different prophages in S. enterica ...
Induction of prophages λ (left-side diagrams) and ϕ24 | Open-i
Induction of prophages λ (left-side diagrams) and ϕ24B (right-side diagrams) in MG1655 hosts bearing the pJW0tet vector (closed ... Mentions: We found that induction of the ϕ24B prophage was delayed by 30-60 min in the presence of the exo-xis region on a ... Mentions: We found that induction of the ϕ24B prophage was delayed by 30-60 min in the presence of the exo-xis region on a ... 4). Contrary to ϕ24B, effects of the exo-xis region on λ prophage induction depended on the nature of the inducing agent. The ...
Evidence of In Vivo Prophage Induction during Clostridium difficile Infection | Applied and Environmental Microbiology
The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of ... prophages from C. difficile strain 630, and a putative prophage from strain ATCC 43255. In fact, besides ϕCD27, the prophage in ... Prophage induction and expression of prophage-encoded virulence factors in group A Streptococcus serotype M3 strain MGAS315. ... We also studied the mobility of ϕMMP02 and ϕMMP04 prophages in vitro. Both prophages were spontaneously induced, with 4 to 5 ...
Plus it
... and also induce prophage. We determined the minimum concentration required for prophage induction and the maximum prophage ... Carcinogens unable to affect bacterial viability are also unable to induce prophage. Failure to induce prophage indicates a ... Phage and Bacterial Inactivation and Prophage Induction by Chemical Carcinogens. NOBUTO YAMAMOTO, M. D. ANDERSON and J. A. ... Phage and Bacterial Inactivation and Prophage Induction by Chemical Carcinogens. NOBUTO YAMAMOTO, M. D. ANDERSON and J. A. ...
Prophage Carriage and Diversity within Clinically Relevant Strains of Clostridium difficile | Applied and Environmental...
Development of a prophage typing system and analysis of prophage carriage in Streptococcus pneumoniae. Appl. Environ. Microbiol ... 1). This could be due to the lack of the correct inducing agent to release the existing prophages, or the prophages in the ... difficile encoding multiple prophages lies in the fact that the presence of a prophage in a bacterial strain may preclude the ... Prophage Carriage and Diversity within Clinically Relevant Strains of Clostridium difficile Jinyu Shan, Krusha V. Patel, Peter ...
Genomic comparison of Escherichia coli O104:H4 isolates from 2009 and 2011 reveals plasmid, and prophage heterogeneity,...
Frontiers | Prophage Rs551 and Its Repressor Gene orf14 Reduce Virulence and Increase Competitive Fitness of Its Ralstonia...
... and nine of the prophages 14 genes including orf14 and six out of seven structural genes (the UW551 prophage mutant), ... and nine of the prophages 14 genes including orf14 and six out of seven structural genes (the UW551 prophage mutant), ... In this study, we determined the effect of the prophage and its ORF14 on the virulence and competitive fitness of its carrier ... existence between a lysogenic phage and its carrier plant pathogenic bacterial strain by determining the effect of the prophage ...
'Ca. Liberibacter asiaticus' Carries an Excision Plasmid Prophage and a Chromosomally Integrated Prophage That Becomes Lytic in...
Ca. Liberibacter asiaticus Carries an Excision Plasmid Prophage and a Chromosomally Integrated Prophage That Becomes Lytic in ... The recently published Ca. L. asiaticus psy62 genome, derived from a psyllid, revealed a prophage-like region of DNA in the ... SC2 also appeared to lack lytic cycle genes and replicated as a prophage excision plasmid, in addition to being found ...
GenomeGenesPhageGenomesInductionStrainsPhagesBacillusPlasmidsBacteriophageCryptic prophageRecombinationBacteriaIsolatesBacteriumSequencesBacteriophagesStaphylococcusLysogenyDefectiveRepressorGenomicChromosomeStrainProteinExcision of the prophagePathogenicityRole of prophageMitomycinPutative prophagesStaphylococcal prophagesEvolution and virulenceSerratiaLactobacillus2017PathogenicEntericaLytic cycleStreptococcusSurvivalVirulence factorColiSpeciesGene expressionChromosomes
Genome40
- A prophage is a bacteriophage (often shortened to "phage") genome inserted and integrated into the circular bacterial DNA chromosome or exists as an extrachromosomal plasmid. (wikipedia.org)
- Pro means ''before'', so, prophage means the stage of a virus in the form of genome inserted into host DNA before being activated inside the host. (wikipedia.org)
- Prophages can provide these bacteria with both resistance mechanisms as well as metabolic advantages which give the host cell the best chance of survival, sometimes even completely altering the bacterial genome. (wikipedia.org)
- Prophage reactivation can occur by recombination between a UV-damaged infecting phage λ chromosome and a homologous phage genome integrated into the bacterial DNA and existing in a prophage state. (wikipedia.org)
- More than 80 genome sequences of staphylococcal bacteriophages and prophages are available in the public genome databases. (asm.org)
- As little is known about the phage diversity in C. diphtheriae in India, the present study was undertaken to investigate the prophages integrated into the genome of 29 clinical isolates of C. diphtheriae using whole-genome shotgun sequencing. (nih.gov)
- Despite the essentially catalytic role of the integrase in both integrative and excisive recombination, RDFs are required to direct the reaction towards excision and to prevent re-integration of the prophage genome when entering a lytic cycle. (mdpi.com)
- Site specific recombination (SSR) constitutes a key step in lysogenic development since it is required for integration as well as for excision of the prophage genome [ 6 ]. (mdpi.com)
- This enables the prophage to be passively replicated with the host genome and ensures that a prophage is present in each of the daughter cells after division. (asm.org)
- Using whole genome sequences of 1,760 isolates of S. enterica representing 151 Salmonella serovars and 66 closely related bacteria, prophage sequences were identified from assembled contigs using PHASTER. (frontiersin.org)
- Prophage sequences were highly variable among S. enterica serovars with a median ± interquartile range (IQR) of 5 ± 3 prophage regions per genome. (frontiersin.org)
- Taken altogether, the diversity of the prophage sequences correlates with genome diversity. (frontiersin.org)
- The data obtained reveal that the horizontal transfer of mobile genetic elements (MGE), such as conjugative transposons and prophages, likely accounts for the great plasticity of the C. difficile genome ( 23 , 39 , 43 ). (asm.org)
- Since damage of the bacterial genome results in prophage induction, the reactivity of each compound with the genome may be indicated by the minimum concentration required for prophage induction and the maximum frequency of prophage induction. (aspetjournals.org)
- The highly flexible genome is genetically diverse and encodes several mobile elements, including transposons and prophages ( 18 , 43 , 47 , 48 ). (asm.org)
- Comparative genome analysis indicates that, while the Georgian strains are the nearest neighbors to the 2011 outbreak isolates sequenced to date, structural and nucleotide-level differences are evident in the Stx2 phage genomes, the mer/tet antibiotic resistance island, and in the prophage and plasmid profiles of the strains, including a previously undescribed plasmid with homology to the pMT virulence plasmid of Yersinia pestis. (nih.gov)
- In this study, we determined the effect of the prophage and its ORF14 on the virulence and competitive fitness of its carrier strain UW551 by deleting the orf14 gene only (the UW551 orf14 mutant), and nine of the prophage's 14 genes including orf14 and six out of seven structural genes (the UW551 prophage mutant), respectively, from the genome of UW551. (frontiersin.org)
- The recently published ' Ca. L. asiaticus' psy62 genome, derived from a psyllid, revealed a prophage-like region of DNA in the genome, but phage have not been associated with ' Ca. L. asiaticus' to date. (apsnet.org)
- Expression of the lysis cassette ( essD, ybcT, rzpD/rzoD ) from the defective lambdoid prophage at the 12th minute of Escherichia coli's genome (DLP12) is required in some strains for proper curli expression and biofilm formation. (microbiologyresearch.org)
- Some phage integrate their genome into the host genome upon infection (prophage), where they may potentially remain indefinitely, coevolving with the host, and providing growth factors and other benefits to the host. (umaine.edu)
- The purpose of my research is to characterize a prophage within the genome of the bacterial host Mycobacterium chelonae Bergey to determine if it is still functional and potentially impacting the fitness of the host bacterium. (umaine.edu)
- The B. anthracis genome sequence contains four putative lambdoid prophages. (biomedcentral.com)
- Prophages, like plasmids, conjugative transposons, insertion sequences, introns and other elements, make up a mobile portion of bacterial genome subject to frequent horizontal exchange that often account for large-scale genomic rearrangements and insertions and deletions in bacterial chromosomes. (biomedcentral.com)
- Prophages are phages that have integrated into the bacterial genome. (phantome.org)
- Some prophages remain active - ready to pop back out of the genome and lyse their unsuspecting hosts. (phantome.org)
- The objectives of this study were to determine the prevalence, diversity and molecular epidemiology of prophages (phage DNA integrated within the bacterial genome) among pneumococci isolated over the past 90 years. (ox.ac.uk)
- We revealed that every pneumococcal genome contained prophage DNA. (ox.ac.uk)
- Prophages typically integrated in one of five different sites within the pneumococcal genome. (ox.ac.uk)
- Abstract Prophages (viral genomes integrated within a host bacterial genome) are abundant within the bacterial world and are of interest because they often confer various phenotypic traits to their hosts, such as by encoding genes that increase pathogenicity. (ox.ac.uk)
- The majority of pathogenic bacteria, including M. tuberculosis , carry viral genomes (prophage) within the bacterial genome that are thought to contribute to virulence. (maineidea.net)
- Previous studies by Davidson's team found that nine proteins encoded in prophage DNA sequences integrated in Pseudomonas aeruginosa's genome had anti-CRISPR activity. (labcritics.com)
- Thus, a prophage is a complete genome of a phage and contains about 100 genes. (thefreedictionary.com)
- The location of a prophage is genetically controlled by its special region, which constitutes about 1/15 of the total length of the genome. (thefreedictionary.com)
- We report here the genome sequence of IL6288, a prophage-free derivative of Lactococcus lactis subsp. (inrae.fr)
- exDNA isolation was found to enrich for circular prophage elements, and qPCR characterization of the strains revealed that such prophage enrichment is detectable only in exDNA samples and would likely be missed in whole-genome DNA preparations (e.g., detection of episomal prophages did not correlate with higher prophage excision rates nor higher excised prophage copy numbers in qPCR experiments using whole-genome DNA). (umaryland.edu)
- In addition, double lysogens were found to be more sensitive to an increased induction stimulus than single lysogens, suggesting that maintenance of a stable prophage is less likely when multiple phage genome copies are present. (microbiologyresearch.org)
- The results reveal numerous differences in both genome structure and gene content among elements derived from different species as well as from strains within species, due in part to the incorporation of additional DNA, or 'morons' into the prophage genomes. (beds.ac.uk)
- Seven prophages were identified within the genome of P. aeruginosa ATCC 27853. (biomedcentral.com)
- The complete genome sequence of P. aeruginosa ATCC 27853 reveals the comprehensive genetic background of the strain, and provides genetic basis for several interesting findings about the functions of surface associated proteins, prophages, and genomic islands. (biomedcentral.com)
- The genomes of w Bol1-b and w Pip are similar in genomic organisation, sequence and gene content, but show substantial differences at some rapidly evolving regions of the genome, primarily associated with prophage and repetitive elements. (biomedcentral.com)
Genes40
- Most bacterial chromosomes contain "cryptic" prophages that have lost genes required for production of phage progeny but retain genes of unknown function that may be important for regulating bacterial host physiology. (asm.org)
- Another important area of interest is the control of prophage gene expression with many of the lysogenic conversion genes (gene conversion) being tightly regulated. (wikipedia.org)
- This description will facilitate an understanding of how foreign genes can be expressed during the process of prophage induction, and a particular example will be described. (asmscience.org)
- Foreign genes are most commonly expressed from a prophage in the lysogenic state. (asmscience.org)
- Possibly, the DNA methylase, endonuclease, or other Lrm1 genes provide a function crucial to L. rhamnosus M1 survival, resulting in the stability of the defective prophage in its lysogenic state. (environmental-expert.com)
- Prophage genes represent the majority of the accessory genes in bacteria genomes and have potential to be used as high discrimination markers in Salmonella . (frontiersin.org)
- Genes from the exo-xis region of λ and Shiga toxin-converting bacteriophages influence lysogenization and prophage induction. (nih.gov)
- We conclude that genes from the exo-xis region of lambdoid bacteriophages participate in the regulation of lysogenization and prophage maintenance. (nih.gov)
- SC2 also appeared to lack lytic cycle genes and replicated as a prophage excision plasmid, in addition to being found integrated in tandem with SC1 in the UF506 chromosome. (apsnet.org)
- These toxin genes are parts of a cryptic prophage, the skin element, and of the prophage SPβ, respectively. (prolekare.cz)
- Prophages often carry genes that confer a selective advantage to the bacterium, typically during host colonization. (prolekare.cz)
- Prophages can convert to infectious viruses through a process known as induction, which is relevant to the spread of bacterial virulence genes. (prolekare.cz)
- The antirepressor genes lie outside the immunity region and are under direct control of the LexA repressor, thus plugging prophage induction directly into the SOS response. (prolekare.cz)
- Early studies also indicated that some genes of certain prophages escape lysogenic repression and express functions that modify the host bacterium. (asmscience.org)
- Genetic element Φ1207.3 (formerly Tn1207.3) is a prophage of Streptococcus pyogenes which carries the macrolide efflux resistance genes mef(A)/msr(D) and is capable of conjugal transfer among streptococci. (le.ac.uk)
- Characterization of this prophage has revealed that multiple genes are conserved with regard to both the DNA and protein sequences. (umaine.edu)
- Multiple structural genes including capsid and tail proteins are also conserved, suggesting the prophage may be capable of producing intact virions. (umaine.edu)
- At least two prophage genes are transcriptionally active. (umaine.edu)
- Expression of these genes suggests that the prophage does indeed have some potential for affecting the biology of the host bacterium. (umaine.edu)
- The role of prophages in the pathogenic life cycle of B. anthracis is not known and the majority of the genes on the B. anthracis prophages do not have an assigned function. (biomedcentral.com)
- For bioinformaticians, prophages are typically only found by looking for genes with homology. (phantome.org)
- We have developed tools that will allow you to identify prophages even if they don't have any similarity to known phage genes. (phantome.org)
- There was limited evidence for genes shared between prophage clusters. (ox.ac.uk)
- 72% of prophages possessed the virulence genes pblA and/or pblB. (ox.ac.uk)
- Overall, pneumococcal prophages were highly prevalent, demonstrated a structured population, possessed genes associated with virulence, and were expressed under experimental conditions. (ox.ac.uk)
- It was therefore proposed that these strains had deletions which removed all or part of the PBSX prophage together with adjacent bacterial DNA encoding the pro ( AB ) and metC genes. (microbiologyresearch.org)
- A prophage generally occupies a specific locus in a bacterial chromosome and is inherited like ordinary bacterial genes. (thefreedictionary.com)
- BR7 also showed increased expression of genes involved in amino acid metabolism and transport, while BR21 showed increased expression of genes involved in iron acquisition and metabolism and in prophage genes. (thefreedictionary.com)
- To obtain efficient recombination of linear donor DNA in recA + or recA − backgrounds, an E. coli strain was made that contains a λ prophage harboring the recombination genes exo , bet , and gam under control of a temperature-sensitive λ cI-repressor (Fig. 1 ). (pnas.org)
- The transfer of these cells from 32°C to 42°C leads to rapid and coherent induction and expression of prophage genes. (nih.gov)
- Methods A series of primers were designed based on potential integration site of SfII and SfX prophages in Shigella flexneri serotype 2b strains , and PCR were performed for 50 serotype 2b strains to amplify special genes located in host and prophages . (bvsalud.org)
- Results In all the serotype 2b strains , prophage SfII and SfX were adjacent to each other, and integrated into the thrW tRNA gene of the host, which were located between genes proA and yaiC of host. (bvsalud.org)
- The altered distribution of RpoZ-defective RNAP was identified mostly within open reading frames, in particular, of the genes inside prophages. (asm.org)
- After analysis of genomewide distribution of wild-type and RpoZ-defective RNAP by the ChIP-chip method, we found alteration of the RpoZ-defective RNAP inside open reading frames, in particular, of the genes within prophages. (asm.org)
- All the observations here described indicate the involvement of RpoZ in recognition of some of the prophage genes. (asm.org)
- We found that accessory genes of ATCC 27853 including prophages and genomic islands (GIs) mainly contribute to the difference between P. aeruginosa ATCC 27853 and other P. aeruginosa strains. (biomedcentral.com)
- 90% of the induced A. baumannii genes were clustered in three prophage regions, and bacteriophage particles were observed after mitomycin C treatment. (moreheadstate.edu)
- These prophages encoded esvI, esvK1, and esvK2, ethanol-stimulated virulence genes previously identified in a Caenorhabditis elegans model, as well as error-prone polymerase alleles. (moreheadstate.edu)
- The induction of all 17978 error-prone polymerase alleles, whether prophage-encoded or not, was recA dependent, but only these DNA polymerase V-related genes were de-repressed in the umuDAb mutant in the absence of DNA damage. (moreheadstate.edu)
- All 3 prophages were significantly similar to GAS prophages that carry virulence factor genes, indicating that these prophages had transferred these genes between pathogens. (biomedsearch.com)
Phage25
- Prophage reactivation in the case of phage λ appears to be an accurate recombinational repair process that is mediated by the recA+ and red+ gene products. (wikipedia.org)
- It is typical for such prophages to be integrated into the bacterial chromosome, but extrachromosomally replicating prophages have been described also, with the best characterized being the Escherichia coli phage P1 system. (asm.org)
- Here, we identify segments of phage genomes that are used for stable extrachromosomal replication in the prophage state. (asm.org)
- Phage fels- 1 and fels- 2 in supernatants were identified by primer/PCR as a putative selective force following single plaque isolations on a prophage-free strain and testing on appropriate hosts. (wiley.com)
- However, in competitions of an archived strain with S . Typhimurium ATCC 14028, phage emerged that had a DNA base sequence segment of prophage ST64B but the sequence differed from the reported homologous segment in ST64B. (wiley.com)
- The time course of phage production was similar to that observed for mitomycin C induction of wild-type prophage. (asm.org)
- Further evidence of C. difficile prophage presence comes from transmission electron microscopy (TEM) analysis of six distinct ribotypes and hybridization analysis of 37 clinical isolates, which both suggested phage carriage was common ( 8 , 11 ). (asm.org)
- Our study is the first toward a better understanding of the co-existence between a lysogenic phage and its carrier plant pathogenic bacterial strain by determining the effect of the prophage Rs551 and its repressor on the virulence and competitive fitness of its carrier strain UW551 of R. solanacearum . (frontiersin.org)
- The paradigm of prophage induction, as set by the phage Lambda model, sees the process initiated by the RecA-stimulated self-proteolysis of the phage repressor. (prolekare.cz)
- In contrast, the Gifsy-2 phage repressor, GtgR, is insensitive to GfoA and GfhA, but is inactivated by an antirepressor from the unrelated Fels-1 prophage (FsoA). (prolekare.cz)
- Since phage and chromosomal sequences near the attachment sites of most prophages are conserved, PCR can be used to assess the phage occupancy of these sites. (asmscience.org)
- Our finding that Las population dynamics derive from the prophage/phage activities may lead to a better understanding of how these bacteria evolve and adapt in different ecological niches. (escholarship.org)
- Excision of prophages could be detected by a PCR based assay for attP sites on extra-chromosomal phage circles and for attB sites on phage-excised chromosomes. (biomedcentral.com)
- All four prophages appear to be defective since, mitomycin C induced culture did not release any viable phage particle or lyse the cells or reveal any phage particle under electron microscopic examination. (biomedcentral.com)
- All four prophages can excise at low frequencies, but are apparently defective in phage production. (biomedcentral.com)
- For phage biologists, prophages have provided years of amusement. (phantome.org)
- Given that nearly all pathogenic bacteria contain prophage, understanding how phage alter pathogenicity and fitness in bacterial hosts is highly relevant to preventing and treating disease. (maineidea.net)
- The proline and methionine requirements and the resistance to mitomycin C were shown to segregate together in phage PBS1-mediated transduction crosses and to be linked to thiB , which is known to be co-transducible with the PBSX prophage. (microbiologyresearch.org)
- Lysis plaques of lambda phage on E. In response to stress, the activated prophage is excised from the DNA of the host cell by one of the newly expressed gene products and enters its lytic pathway. (alitcg.com)
- The prophage of a λ-phage, for example, is localized in a chromosome of colibacillus together with the gene that controls the splitting of galactose. (thefreedictionary.com)
- Co-transcriptional DNA and RNA targeting by type III-A CRISPR-Cas systems restricts temperate phage lytic infections while allowing lysogenic infections to be tolerated under conditions where the prophage targets are transcriptionally repressed. (elsevier.com)
- Recently, we identified plasmidial and episomal prophages in S. aureus strains using an extra-chromosomal DNA (exDNA) isolation and sequencing approach, uncovering the plasmidial phage ϕBU01, which was found to encode important virulence determinants. (umaryland.edu)
- The results from these studies will lead us to understand the role of prophages in diseases caused by S. aureus, and how we can make S. aureus more susceptible to immune responses by blocking the phage contribution mechanism. (elsevier.com)
- During phage-mediated replication and host cell lysis, the toxins are released en masse from the bacterial cells, and the severity of disease is linked inexorably to toxin load. (microbiologyresearch.org)
- Thirty markers were selected from prophages ST64B, ST64T, ST104, P22, Gifsy-1, sopEΦ and mostly phage-related AFLP fragments, and organised into two multiplex PCRs of 15 markers each. (ed.ac.uk)
Genomes16
- Temperate bacteriophages can integrate their genomes into the bacterial host chromosome and exist as prophages whose gene products play key roles in bacterial fitness and interactions with eukaryotic host organisms. (asm.org)
- Prophages are integrated viral genomes in bacteria. (polsinelli.com)
- The name bacteriophage encompasses all bacterial viruses, including temperate phages which have the particularity to integrate their genomes into their hosts, becoming prophages. (mdpi.com)
- We detected 154 different prophages in S. enterica genomes. (frontiersin.org)
- Prophages are encoded in most genomes of sequenced Clostridium difficile strains. (asm.org)
- Many species of bacteria harbor multiple prophages in their genomes. (prolekare.cz)
- Here we show that a large family of lambdoid prophages found in Salmonella genomes employs an alternative induction strategy. (prolekare.cz)
- The results indicated that functional prophage genomes may be a common constituent of all bacterial genomes of the investigated strains. (semanticscholar.org)
- We have identified the prophages in the complete genomes, and we provide that data for you. (phantome.org)
- Download prophage prediction s for selected genomes. (phantome.org)
- These are the genomes where we have reliable training sets of what prophages should look like. (phantome.org)
- Download predicted prophage proteins from all genomes. (phantome.org)
- Nearly 500 pneumococcal genomes were investigated and RNA sequencing was used to explore prophage gene expression. (ox.ac.uk)
- 1,300 genomes of 70 different Streptococcus species for evidence of prophages and identified nearly 800 prophages and satellite prophages, the majority of which are reported here for the first time. (ox.ac.uk)
- Here we report that over one-third of pneumococcal genomes possessed satellite prophages and demonstrate for the first time that a satellite prophage was associated with virulence in a murine model of infection. (ox.ac.uk)
- Pathogenic species of the bacterial genus Mycobacterium , including M. tuberculosis , carry these integrated viral genomes (prophage) that are hypothesized to contribute to virulence. (maineidea.net)
Induction32
- Upon detection of host cell damage by UV light or certain chemicals, the prophage is excised from the bacterial chromosome in a process called prophage induction. (wikipedia.org)
- Zygotic induction occurs when a bacterial cell carrying the DNA of a bacterial virus transfers its own DNA along with the viral DNA (prophage) into the new host cell. (wikipedia.org)
- The variety of types suggests that the cryptic prophage is mutagenized as a consequence of the induction process. (genetics.org)
- Importantly, for lysogenic conversion to occur, it is not necessary that the prophage remains functional as a virus that is capable of prophage induction or lytic growth. (asmscience.org)
- An induction mechanism which involves recombination at the prophage insertion site is proposed to explain these differences. (asm.org)
- In this report, using bacteriophage λ and Shiga toxin-converting bacteriophage ϕ24Β, we demonstrate that the presence of this region on a multicopy plasmid results in impaired lysogenization of Escherichia coli and delayed, while more effective, induction of prophages following stimulation by various agents (mitomycin C, hydrogen peroxide, UV irradiation). (nih.gov)
- Spontaneous induction of λ and ϕ24Β prophages was also more efficient in bacteria carrying additional copies of the corresponding exo-xis region on plasmids. (nih.gov)
- Induction of prophages λ (left-side diagrams) and ϕ24B (right-side diagrams) in MG1655 hosts bearing the pJW0tet vector (closed squares) or either pGAW3775tet (left-side diagrams) or pSBe.x.r.ϕ24B (right-side diagrams) (open squares), treated with 0.2 μg/ml mitomycin C (a), 50 J/m2 UV irradiation (b), or 1 mM H2O2 (c) at time 0. (nih.gov)
- We found that induction of the ϕ24B prophage was delayed by 30-60 min in the presence of the exo-xis region on a multicopy plasmid, but this process was finally more efficient than that in control experiments, as more progeny phages were produced (Fig. 4). (nih.gov)
- This phenomenon occurred irrespective of the kind of the inducer used, nevertheless, the delay in prophage induction was the longest (60 min) in experiments with mitomycin C (Fig. 4). (nih.gov)
- Contrary to ϕ24B, effects of the exo-xis region on λ prophage induction depended on the nature of the inducing agent. (nih.gov)
- Nevertheless, with exception of the induction with mitomycin C, final efficiency of induction of λ prophage was higher in the presence of the exo-xis region on a plasmid relative to control experiments (Fig. 4). (nih.gov)
- We therefore provide, for the first time, evidence of in vivo prophage induction during CDI. (asm.org)
- We determined the minimum concentration required for prophage induction and the maximum prophage induction frequency for each carcinogen. (aspetjournals.org)
- The ability of antirepressors to recognize non-cognate repressors allows coordination of induction of multiple prophages in polylysogenic strains. (prolekare.cz)
- Results: Lysogen cultures propagated for 5-6 hours produced a high cell density with a low proportion of spontaneous prophage induction events. (ufl.edu)
- SOS-induced spontaneous prophage induction in Corynebacterium glutamicum", Presented at the EMBL Heidelberg "New Approaches and Concepts in Microbiology", Heidelberg, Germany, 2013. (uni-bielefeld.de)
- SOS-induced spontaneous prophage induction in Corynebacterium glutamicum", Presented at the Nikon Symposium on Advanced Imaging in Cell- and Microbiology Technology and Applications, Jülich, Germany, 2013. (uni-bielefeld.de)
- Induction with mitomycin C increased the frequency of excision of one of the prophages by approximately 250 fold. (biomedcentral.com)
- Heat-resistant derivatives of a Bacillus subtilis 168 strain carrying an xhi mutation, which causes heat-sensitive induction of the PBSX prophage, have been isolated and screened for the acquisition of auxotrophy. (microbiologyresearch.org)
- Lysogeny and prophage induction in coastal and offshore bacterial communities. (thefreedictionary.com)
- Host responses influence on the induction of lambda prophage. (nih.gov)
- Prophage induction can be initiated either by DNA damage or by heat treatment of a temperature-sensitive repressor. (nih.gov)
- Prophage induction by heat shock at 42°C.A. Strains W3110 (λcI857knR) carrying the pR-gfp (orange), the pE-gfp (blue) or the pR′-tR′-gfp (red) plasmids were grown exponentially in minimal medium, heat induced at 42°C for 5, 10 or 15 min (light to darker colours respectively), and assayed for fluorescence at 32°C for the indicated times. (nih.gov)
- Spontaneous prophage induction in a small subpopulation of cells which takes place in the absence of a known stimulus is an often observed, but poorly understood phenomenon. (tu-bs.de)
- With the proposed project we aim to investigate the impact of stress responses and stochasticity fluctuations of key regulatory proteins on the spontaneous induction of the Corynebacterium glutamicum prophage CGP3 at the single cell level. (tu-bs.de)
- We aim to combine classical microbiological approaches with stochastic modelling and the design of novel microfluidic devices for single cell studies to contribute to a better understanding of spontaneous prophage induction as a general phenomenon in bacterial populations. (tu-bs.de)
- Toxin gene expression in double lysogens was in excess of the single lysogen counterpart, both in the prophage state and after induction of the lytic life cycle. (microbiologyresearch.org)
- Prophage Induction and Differential RecA and UmuDAb Transcriptome Regu" by Janelle M. Hare, Joshua C. Ferrell et al. (moreheadstate.edu)
- Investigation of this phenomenon revealed that the non-monotonic response was associated with prophage induction, which facilitated killing of S. aureus persisters. (princeton.edu)
- Elimination of prophage induction with tetracycline was found to prevent cell lysis and persister killing. (princeton.edu)
- The instance Induction of prophage lambda by chlorinated organics : detection of some single-species/single-site carcinogens, David M. DeMarini and Harold G. Brooks, (microform) represents a material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries . (missouri.edu)
Strains17
- Prophages are able to do a multitude of things within their respective bacterial strains. (wikipedia.org)
- Prophages can increase the virulence potential of bacterial strains in both humans and plant pathogens as well as increase the ability of the bacteria to survive in harsh environments. (wikipedia.org)
- have found that most TSS strains harbour prophages with common plating characteristics and suggest that the toxin(s) involved in TSS are transmitted by lysogenic conversion. (nih.gov)
- Prophage was induced when strains of Bacillus subtilis 168 lysogenic for φ105c4 were grown to competence and exposed to specific bacterial DNAs. (asm.org)
- In this study, the prophage sequence diversity in different Salmonella serovars and genetically related strains was investigated. (frontiersin.org)
- While some prophage sequences were highly conserved among the strains of specific serovars, few regions were lineage specific. (frontiersin.org)
- Therefore, strains belonging to each serovar could be clustered separately based on their prophage content. (frontiersin.org)
- Some mammalian carcinogens and their metabolites affect the viability of Salmonella typhimurium strains, as indicated by a decrease in colony formation, and also induce prophage. (aspetjournals.org)
- The majority of these phages are not amenable to propagation, and therefore the development of a molecular marker is a useful tool with which to establish the extent and diversity of C. difficile prophage carriage within clinical strains. (asm.org)
- This study reveals the high incidence of prophage carriage in clinically relevant strains of C. difficile and correlates the molecular data to the morphological observation. (asm.org)
- Interestingly, we found that the wt ϕRs551-carrying strain UW551 of R. solanacearum significantly outcompeted the wt strain RUN302 which lacks the prophage in tomato plants co-inoculated with the two strains. (frontiersin.org)
- Enteric species like E. coli and Salmonella typically contain multiple resident prophages whose variability in number and assortment constitutes a major source of diversity between strains [1] - [3] . (prolekare.cz)
- The retention of all four putative prophage regions across all tested strains of B. anthracis is further evidence of the very recent emergence of this lineage and the prophage regions may be useful for differentiating the B. anthracis chromosome from that of its neighbors. (biomedcentral.com)
- The defective prophage can be moved to other strains and can be easily removed from any strain. (pnas.org)
- Objective To study the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains . (bvsalud.org)
- Prophage SfX was located immediately upstream of prophage SfII in all the detected 50 serotype 2b strains exception for strain 51251. (bvsalud.org)
- Conclusion This was the first report on the integration site and arrangement of serotype -converting prophages SfII and SfX in Shigella flexneri 2b strains . (bvsalud.org)
Phages6
- The presence of a defective prophage in an industrial strain could provide superinfection immunity to the host but could also contribute DNA in recombination events to produce new phages potentially infective for the host strain in a large-scale fermentation environment. (environmental-expert.com)
- Recent studies have highlighted the great diversity of prophages in the clinical isolates of C. difficile ( 17 , 36 , 42 ), but only 5 fully characterized phages with complete genomic sequences are available in public databases. (asm.org)
- GfoA and GfhA, the antirepressors of Salmonella prophages Gifsy-1 and Gifsy-3, each target both of these phages' repressors, GfoR and GfhR, even though the latter proteins recognize different operator sites and the two phages are heteroimmune. (prolekare.cz)
- As an intracellular bacterium, ' Candidatus Liberibacter asiaticus' (Las) lacks known transposons and IS elements but contains at least two prophages/phages. (escholarship.org)
- We undertook this study in order to understand whether the four prophages are unique to B. anthracis and whether they produce active phages. (biomedcentral.com)
- The prophages of certain bacterial phages an. (bookrags.com)
Bacillus4
- Prophage-triggered membrane vesicle formation through peptidoglycan damage in Bacillus subtilis. (sigmaaldrich.com)
- The structure of Bacillus subtilis SPβ prophage dUTPase and its complexes with two nucleotides. (ox.ac.uk)
- Bacillus subtilis has both a genomic and an SPβ prophage homotrimeric dUTPase. (ox.ac.uk)
- In molecular biology, the YqaJ refers to the YqaJ/K domain from the skin prophage of the bacterium, Bacillus subtilis. (wikipedia.org)
Plasmids1
- We describe a suite of plasmids based on these prophage replication functions that vary in copy number, stability, host range, and compatibility. (asm.org)
Bacteriophage3
- Bacteriophage λ is able to undergo a type of recombinational repair called prophage reactivation. (wikipedia.org)
- Prophage is the deoxyribonucleic acid (DNA) of a temperate bacteriophage combined with the DNA of a lysogenized bacterium. (thefreedictionary.com)
- It is unclear whether all of these putative prophages are active, although one (ΦK96243) was shown to be a productive bacteriophage. (beds.ac.uk)
Cryptic prophage1
- Deletion of O‐island 51, a 14.93 kb cryptic prophage (CP‐933C), resulted in a reduction in LEE expression and T3S. (qub.ac.uk)
Recombination4
- Recombination directionality factors (RDFs), or excisionases, are essential players of prophage excisive recombination. (mdpi.com)
- Sequence analysis of these variants revealed the variations were due to the recombination and reassortment between two prophages. (escholarship.org)
- A temperature-dependent repressor tightly controls prophage expression, and, thus, recombination functions can be transiently supplied by shifting cultures to 42°C for 15 min. (pnas.org)
- The efficient prophage recombination system does not require host RecA function and depends primarily on Exo, Beta, and Gam functions expressed from the defective λ prophage. (pnas.org)
Bacteria8
- With data from more nonpathogenic bacteria, researchers will be able to gather evidence as to whether or not prophages contribute to the survival value of the host. (wikipedia.org)
- Prophage genomics has the potential to lead to ecological adaptations of the relationships between bacteria. (wikipedia.org)
- which suggests the potential application of the holin gene to study prophage carriage in other bacteria. (asm.org)
- Some prophages get stuck inside the bacteria, and eventually get deleted by natural selection. (phantome.org)
- However, in some growing lysogenic bacteria (approximately one cell per million), the prophage becomes infectious, that is, it becomes induced. (thefreedictionary.com)
- Individual lysogenic bacteria may carry several prophages. (thefreedictionary.com)
- To overcome these challenges, we developed an internally controlled quantitative PCR (Ter-qPCR) that targets the multicopy terminase large subunit ( terL ) gene encoded by prophages that are only found in LD-causing bacteria. (cdc.gov)
- Prophage encoded markers are prevalent in many other pathogenic bacteria rendering this approach highly applicable to bacterial identification in general. (cdc.gov)
Isolates8
- The prevalence of prophages in a representative S. aureus strain collection consisting of 386 isolates of diverse origin was determined. (asm.org)
- Insights to the diphtheria toxin encoding prophages amongst clinical isolates of Corynebacterium diphtheriae from India. (nih.gov)
- Analysis of S . Enteritidis isolates from seven outbreaks generated distinct prophage profiles for each outbreak. (frontiersin.org)
- More than 300 geographically and temporally divergent isolates of B. anthracis and its near neighbors were screened by PCR for the presence of specific DNA sequences from each prophage region. (biomedcentral.com)
- In this study, we performed spa typing and RSTing with 84 isolates from mastitic cows (22 farms, 72 cows, and 84 udders) and developed a molecular prophage typing (mPPTing) method for molecular epidemiological analysis of bovine mastitis. (bvsalud.org)
- This bacterium is so closely associated with prophages that it is rare to find S. aureus isolates without prophages. (elsevier.com)
- Individual isolates of Burkholderia pseudomallei and B. thailandensis , for example, carry a variety of strain-specific genomic islands (GIs), including putative pathogenicity and metabolic islands, prophage-like islands, and prophages. (beds.ac.uk)
- Isolates were divided into sixteen main prophage marker profile types. (ed.ac.uk)
Bacterium3
- Prophages can tell researchers a lot about the relationship between a bacterium and a host. (wikipedia.org)
- In this study, we revealed the genetic diversity and population dynamics of this bacterium based on two prophage hyper-variable regions (HVRs) using separate libraries constructed from citrus, periwinkle and psyllid. (escholarship.org)
- Secondly, the concept of prophage was applied to resemble obfuscation technique virus DNA during the injection process in the operating system files, similar injection of a virus in a bacterium. (thefreedictionary.com)
Sequences2
- Preliminary analyses confirmed that the sequences of the three prophages diverge considerably in the portion corresponding to the immunity module. (asmscience.org)
- The two HVRs, Type A and B, share highly conserved sequences and are localize to the two prophages, FP1 and FP2, respectively. (escholarship.org)
Bacteriophages2
- Temperate bacteriophages are common and establish lysogens of their bacterial hosts in which the prophage is stably inherited. (asm.org)
- Contributions of P2- and P22-like prophages to understanding the enormous diversity and abundance of tailed bacteriophages. (semanticscholar.org)
Staphylococcus1
- Temperate Prophages Increase Bacterial Adhesin Expression and Virulence in an Experimental Model of Endocarditis Due to Staphylococcus aureus From the CC398 Lineage. (unil.ch)
Lysogeny3
- Define plaque lysogeny and prophage stage. (alitcg.com)
- Crucial in the maintenance of lysogeny and prophage stability. (alitcg.com)
- Answer to Define plaque, lysogeny, and prophage. (alitcg.com)
Defective4
- Interestingly, at the same locus in the B. cereus 14579 chromosome, is a defective prophage, phi6A53, with little similarity to B. anthracis lambdaBa0l. (biomedcentral.com)
- The defective prophage pool of Escherichia coli O157: prophage-prophage interactions potentiate horizontal transfer of virulence determinants. (thefreedictionary.com)
- A defective λ prophage supplies functions that protect and recombine an electroporated linear DNA substrate in the bacterial cell. (pnas.org)
- Since all 10 prophages in E. coli K-12 carry only a small number of promoters, the altered occupancy of RpoZ-defective RNAP and of transcripts might represent transcription initiated from as-yet-unidentified host promoters. (asm.org)
Repressor2
- The DNA of the bacterial cell is silenced before entry into the cell by a repressor protein which is encoded for by the prophage. (wikipedia.org)
- Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic rac Prophage of Escherichia coli K-12. (ncbs.res.in)
Genomic3
- Prophages are distinct from other genomic segments encoding virulence factors that have been acquired by horizontal gene transfer events. (polsinelli.com)
- For instance, 11% of the genomic DNA of strain 630 is made up of MGE, including 8 conjugative transposons and 2 functional and highly similar prophages ( 39 ). (asm.org)
- In parallel, we analyzed, using genomic SELEX (systemic evolution of ligands by exponential enrichment), the distribution of constitutive promoters that are recognized by RNAP RpoD holoenzyme alone and of general silencer H-NS within prophages. (asm.org)
Chromosome3
- The prophage is passively replicated as part of the host chromosome as long as conditions are not threatening to the host, in which case the prophage shifts to a lytic development [ 3 , 4 , 5 ]. (mdpi.com)
- The DNA of a prophage is approximately 1/50 to 1/100 the size of the DNA of a bacterial chromosome and contains about 10 5 pairs of nucleotides. (thefreedictionary.com)
- A prophage is nonpathogenic for a bacterial cell and is replicated over many generations simultaneously with the bacterial chromosome. (thefreedictionary.com)
Strain2
- ZK, CP000001) revealed that prophages are generally inserted at different chromosomal loci than B. anthracis , except in B. cereus ZK strain that contains an element very similar to B. anthracis lambdaBa01 inserted at the same locus. (biomedcentral.com)
- lactis strain IL1403, and confirm precise deletion of all prophages. (inrae.fr)
Protein4
- While studying transcription factors encoded in horizontally acquired regions in E. coli, we realized that the protein RacR, a putative transcription factor encoded by a gene on the rac prophage, is an essential protein. (ncbs.res.in)
- The Corynebacterium glutamicum ATCC 13032 prophage CGP3 encodes an actin-like protein, AlpC that was shown to be involved in viral DNA transport and efficient viral DNA replication. (uni-muenchen.de)
- Below are the list of possible Prophage antitermination protein products. (mybiosource.com)
- Also known as Prophage antitermination protein Q homolog QuuD (Antitermination protein Q homolog from lambdoid prophage DLP12). (mybiosource.com)
Excision of the prophage1
- In S. aureus MSSA476, we found that enrichment and excision of the prophage ϕSa4ms into the cytoplasm was temporal and that episomal prophage localization did not appear to be a precursor to lytic cycle replication, suggesting ϕSa4ms excision into the cytoplasm may be part of a novel lysogenic switch. (umaryland.edu)
Pathogenicity1
- This presents us with an alternative mechanism for the role of prophage in host virulence and pathogenicity. (maineidea.net)
Role of prophage1
- This project aims to characterize the impact of prophage on mycobacterial fitness and virulence by exploring the role of prophage activity in altering host gene expression. (maineidea.net)
Mitomycin1
- Prophage Lrm1 was induced with mitomycin C from an industrial Lactobacillus rhamnosus starter culture, M1. (environmental-expert.com)
Putative prophages1
- Here we present the results of analysis of 37 prophages, putative prophages, and prophage-like elements from six different Burkholderia species. (beds.ac.uk)
Staphylococcal prophages1
- Here we established a classification scheme for staphylococcal prophages of the major Siphoviridae family based on integrase gene polymorphism. (asm.org)
Evolution and virulence1
- Prophages contribute to the evolution and virulence of most bacterial pathogens, but their role in Clostridium difficile is unclear. (asm.org)
Serratia3
- Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. (nih.gov)
- The Serratia entomophila antifeeding prophage (Afp) is a bullet-shaped toxin-delivery apparatus similar to the R-pyocins of Pseudomonas aeruginosa. (nih.gov)
- Here, we characterize the antifeeding prophage AFP from Serratia entomophila by cryo-electron microscopy. (pdbj.org)
Lactobacillus1
- Spontaneously induced prophages in Lactobacillus gasseri contribute to horizontal gene transfer. (semanticscholar.org)
20171
- Welcome to the Prophage blog 2017! (blogspot.hr)
Pathogenic2
- Through RNAseq analysis of both pathogenic ( M. chelonae ) and non-pathogenic ( M. smegmatis ) mycobacterial species, we have determined that prophage significantly affect host gene expression. (maineidea.net)
- Determining how prophage within non-tuberculosis pathogenic mycobacteria contribute to virulence will provide opportunities to develop and improve treatment of bacterial diseases. (maineidea.net)
Enterica1
- Prophage repertoires provide an additional marker for differentiating S. enterica subtypes during foodborne outbreaks. (frontiersin.org)
Lytic cycle1
- The fitness costs depend on the activity of prophage-internal promoters and type III-A Cas nucleases implicated in targeting, can be more severe in double lysogens, and are alleviated by spacer-target mismatches which do not abrogate immunity during the lytic cycle. (elsevier.com)
Streptococcus1
- Overall, our findings demonstrate that prophages are widespread components of Streptococcus species and suggest that they play a role in pneumococcal pathogenesis. (ox.ac.uk)
Survival2
- Satellite prophages are 'parasites of parasites' that rely on the bacterial host and another helper prophage for survival. (ox.ac.uk)
- It can be seen that the results for Prophage were slightly better than Avastin alone and Avastin plus irinotecan in terms of median overall survival and that Prophage seemed meaningfully better in terms of six month survival. (smithonstocks.com)
Virulence factor1
- GGS_124 contained 3 prophages, with one containing a virulence factor gene for streptodornase. (biomedsearch.com)
Coli3
- The cryptic rac prophage in Escherichia coli K-12 carries the gene for a putative transcription factor RacR, whose deletion is lethal. (ncbs.res.in)
- This study advances understanding of not only the regulatory role of omega subunit in the functions of RNAP but also the regulatory interplay between prophages and the host E. coli for adjustment of cellular physiology to a variety of environments in nature. (asm.org)
- Identification of a novel prophage regulator in Escherichia coli controlling the expression of type III secretion. (qub.ac.uk)
Species1
- There was convincing evidence that cross-species transmission of prophages is not uncommon. (ox.ac.uk)
Gene expression2
- RNA sequencing provided clear evidence of prophage gene expression. (ox.ac.uk)
- In this research proposal, we test the hypothesis that prophages can increase staphylococcal virulence not only by providing virulence factors but also by altering bacterial gene expression. (elsevier.com)
Chromosomes1
- Furthermore, identical prophages were found in the chromosomes of C. difficile isolated from the corresponding fecal samples. (asm.org)