No data available that match "progression combinations of alleles double strand meiotic prophase i"


2 exert their regulatory functions during meiotic prophase progression, gametogenesis, and oocyte maturation. In addition to ... RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and ... 2008). "A loss of function allele for murine Staufen1 leads to impairment of dendritic Staufen1-RNP delivery and dendritic ... These sequences are then arranged in varying combinations to fulfill the need for diversity. A specific protein's recognition ...
... that meiotic recombination is initiated by the formation of DNA double-strand breaks (DSBs) during early prophase I. The ... Recombination occurs as a programmed event that results in new combinations of parental alleles via crossover and non-crossover ... Using high throughput sequencing we revealed Set1C independent Spp1 subpopulation during meiotic progression with different ... Early scientific research considered R-loops as potential hotspots for genome instability as their single stranded part is ...
2008 Spo11 and the formation of DNA double-strand breaks in meiosis. Genome Dyn. Stab. 2: 81-123. ... 1990 The b alleles of U. maydis, whose combinations program pathogenic development, code for polypeptides containing a ... 2012 The molecular control of meiotic chromosomal behavior: events in early meiotic prophase in Drosophila oocytes. Annu. Rev. ... 2000 Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and ...
BuhlerC, BordeV, LichtenM (2007) Mapping meiotic single-strand DNA reveals a new landscape of DNA double-strand breaks in ... 2011) A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. Cell ... its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new ... As a consequence of this gene conversion bias, an allele with a higher propensity toward DSB formation will tend to be under- ...
... an asymmetric intermediate at the double-strand break to double-holliday junction transition of meiotic recombination. Cell 106 ... Mutation of RED1 completely suppressed the meiotic progression defect of mus8. mek1 also permitted meiotic progression of mus81 ... Alleles marked with kanMX4 were disrupted by PCR using the method of Longtine et al. (1998). The mus81Δ::ARG4 allele was ... 1993 Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis. J. Cell Biol. 121: 241-256. ...
BuhlerC, BordeV, LichtenM (2007) Mapping meiotic single-strand DNA reveals a new landscape of DNA double-strand breaks in ... E) Sporulation frequencies at 24 hours in smc5 and nse4 mutants in combination with mutants that show robust prophase I arrest ... Depletion of Smc5 or Nse4 had no effect on the meiotic progression in any of these mutants (Figure 8E). Combining the dmc1Δ ... This is in sharp contrast to the smc6-9 temperature sensitive allele, which was previously shown to cause meiotic catastrophe ...
... or different PAH mutant alleles in a strain background (SK1) that exhibits synchronous meiotic progression (see Table 1). These ... comp, 150-fold excess of unlabeled competitor URS1SPO13 double-stranded oligonucleotide added to the reaction. (B) Complex ... Sin3p is required for the execution of the first meiotic nuclear division (30), with mutants arresting in meiotic prophase I ( ... Δ alleles individually or in combination. Mid-log-phase SPO13 mRNA levels were determined in each strain background by S1 ...
Drosophila mus301/spindle-C encodes a helicase with an essential role in double-strand DNA break repair and meiotic progression ... indicating that the defects are a result of unrepaired meiotic DSBs. We also tested a double mutant genotype combination with ... exhibit an accumulation of γ-H2AV foci that persist throughout meiotic prophase, corresponding to unrepaired meiotic DSBs (Fig ... Other alleles analyzed in this study include the following: mei-41D3 (Laurençon et al., 2003), mei-W684572 (Jang et al., 2003 ...
1990) A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae. Cell 61: ... Spermatogenesis is arrested in prophase I inAtm −/− spermatocytes.The progression of the meiotic cell cycle in Atm −/− and ... 1997) Partial rescue of the prophase 1 defects of Atm-deficient mice by p53 and p21 null alleles. Nat. Genet. 17:462-466. ... SC immunostaining in combination with telomere FISH to these nuclei revealed fragmentary, strong SCP3 signals at and around the ...
Human PRDM9 zinc finger domains of alleles A (ZA) and I (ZI) interact specifically with double-stranded oligonucleotides ... 3). Analysis of Prdm9-/- mice has shown that Prdm9 is essential for progression through meiotic prophase (20). On the basis of ... 1B). Due to the low predicted specificity of some zinc fingers and the multiple combinations through which several zinc fingers ... allele A is identical to the genome sequence reference allele (allele B), which is at a frequency of 5%. Among other alleles ( ...
We propose that this function of Cdc28 helps to coordinate the events of meiotic prophase with each other and with progression ... Diaz RL, Alcid AD, Berger JM, Keeney S. Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break ... Tagged and untagged MER2 alleles were integrated at mer2δ::kanMX4 by linearization of vectors with EcoRI and yeast ... Combinations of orientations used here were as follows: LexA-cMer2/cMer2-Gal4AD; LexA-Mei4/cMer2-Gal4AD; Gal4AD-Rec114/LexA- ...
Proper repair of double-strand breaks (DSBs) is key to ensure proper chromosome segregation. In this study, we found that the ... Mek1 kinase in budding yeast regulates interhomolog recombination and coordinates meiotic progression with double-strand break ... Schild D (1995) Suppression of a new allele of the yeast RAD52 gene by overexpression of RAD51, mutations in srs2 and ccr4, or ... Klein HL (2001) Mutations in recombinational repair and in checkpoint control genes suppress the lethal combination of ...
2016) MEIOTIC F-BOX is essential for male meiotic DNA double-strand break repair in rice. Plant Cell 28: 1879-1893. ... 2006) Reduced meiotic crossovers and delayed prophase I progression in AtMLH3-deficient Arabidopsis. EMBO J 25: 1315-1323. ... Both alleles produced sterile plants with similar meiotic defects (Supplemental Fig. S4, B and C). The osptd-1 mutant allele ... Different antibody combinations were diluted (1:50-1:500, depending on the antibody titer) in TNB buffer (0.1 m Tris-HCl, pH ...
2 exert their regulatory functions during meiotic prophase progression, gametogenesis, and oocyte maturation. In addition to ... RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and ... 2008). "A loss of function allele for murine Staufen1 leads to impairment of dendritic Staufen1-RNP delivery and dendritic ... These sequences are then arranged in varying combinations to fulfill the need for diversity. A specific proteins recognition ...
C. elegans germ cells switch between distinct modes of double-strand break repair during meiotic prophase progression PLOS ... Our data suggest that the CO landscape is shaped by a combination of three attributes of the SC central region: a CO-promoting ... Our characterization of new him-3 alleles reveals previously unknown functions for the protein. HIM-3 is required for the ... Finally, we have exploited S-phase labeling to monitor the timing of progression through meiotic prophase. Meiotic prophase for ...
3A). In the germline, GFP::KDP-1 was NE-enriched in the distal mitotic zone and all stages of meiotic prophase, but most ... Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806-811. ... unc-84(n369), a null allele (Malone et al., 1999), and unc-84(RNAi) larvae and embryos did not have noticeably different GFP:: ... The major phenotype seen in kdp-1(RNAi) gonads was a combination of large chromatin masses and extra chromatin bodies in ...
... a germ cell-specific gene activated during meiotic commitment. Over a 21-day ablation phase induced by the HSVtk pro-drug, ... 3g), which is known to localize to DNA double-strand breaks in germ cells during the early stages of meiotic prophase76. This ... Notably, our assessment of meiotic activation and progression during the post-GCV recovery phase (Fig. 3e-g) in turn ... We then introduced two additional alleles into pStra8-rtTA mice: 1) a tetracycline responsive element (TRE)-driven Cre ...
Cells lacking RAD50 do not form meiotic double-strand breaks (6), yet they still arrested in G2 with low levels of Clb3 protein ... meiotic prophase) separates premeiotic DNA replication from the first meiotic division. During meiotic prophase, homologous ... we analyzed the consequences on meiotic progression when benomyl was added to a meiotic culture at the time of meiotic ... Pairing was then lost as cells completed the first meiotic division. The nonhomologous LEU2/URA3 combination, on the other hand ...
... a specific isoform of the meiotic double-strand break catalyst SPO11 (Baudat et al., 2000; Keeney et al., 1997) is needed ( ... Prophase I progression. To evaluate prophase I progression in Spo11β-onlymb (both Mad2+/+ and Mad2+/−), Mad2+/− and control ... The combination of these two antibodies allows identification of prophase I sub-stages (leptonema, zygonema, pachynema, ... To examine whether disruption of one Mad2 allele affects sex chromosome pairing in Spo11β-onlymb mice, we scored X-Y pairing ...
In contrast, expression of double-stranded hairpin RNA from a 1.688X repeat generated abundant siRNA and dramatically increased ... Study of the phenotype of different polo allele combinations compared to the effect of chemical inhibition revealed significant ... suggest that DMRT6 represses genes involved in spermatogonial differentiation and activates genes required for meiotic prophase ... Histone H3.3 is required to maintain replication fork progression after UV damage. Curr Biol 24: 2195-2201. PubMed ID: 25201682 ...
2011). Double-strand breaks in heterochromatin move outside of a dynamic HP1a domain to complete recombinational repair. Cell ... NSE4A-VENUS accumulated strongly in female meiocytes initiating meiotic prophase I (Figure 2E, flower stage 11, arrowhead). The ... This allele alleviates the problem of homozygous lethality encountered in the loss-of-function allele nse4a-1, thereby enabling ... G) G2/M cell cycle progression in nse4a-2 and nse4b-2 analyzed by ProCycB1;1:CycB1;1:GUS (CycB1;1-GUS) after exposure to 10 µM ...
γ-HIS2AV foci persist into late stages of meiotic prophase in okr and spnB mutants. Following region 3 in the germarium, each ... Hall, J. C. (1972). Chromosome segregation influenced by two alleles of the meiotic mutant c(3)G in Drosophila melanogaster. ... Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11. Mol. Cell 6, 989-998. ... A combination of two Orb antibodies (4H8 and 6H4) (Lantz et al., 1994) was used at 1:150 and a FITC-labeled goat anti-mouse ...
Moreover, myt1/Df(3L)64D-F hemizygotes display identical phenotypes as transheterozygous combinations of the original alleles, ... or double-Bar eye (two copies of the B allele), yellow females (from FM7,y,B/FM7,y,B eggs) or females with normal eyes and ... To determine at which meiotic division(s) the X chromosome NDJ occurred in myt1 mutant females, FM7, y, B/y; myt11/myt12 ... Jackman, M., Lindon, C., Nigg, E. A. and Pines, J. (2003). Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat ...
The stages of meiotic progression are indicated. fancd2-1 atm-2 and mus81-2 atm-2 double mutants are similarly affected with ... 2011). Meiotic double-strand breaks occur once per pair of (sister) chromatids and, via Mec1/ATR and Tel1/ATM, once per quartet ... Thus, fancd2-1 exhibits an additive effect in combination with both msh4 and mus81-2. It has been shown before that msh4 and ... To investigate this further, we generated a triple mutant line combining the msh4, mus81-2, and fancd2-1 mutant alleles. The ...
During meiotic recombination, DNA double-strand breaks (DSBs) are deliberately induced, and a subset of the breaks are repaired ... elegans early meiotic prophase. Subsequently, the clustered chromosomes are redispersed into aligned homologs upon SC assembly ... Using a combination of tandem mass spectrometry and methyl-specific antibodies, I find that Set9 methylates FoxO3 at a single ... were probed by monitoring the repair outcome of a DSB induced at a defined site at different stages of meiotic progression, in ...
Geneticists for several decades knew that meiotic crossover and recombination is absent in Drosophila males and some female ... Geneticists for several decades knew that meiotic crossover and recombination is absent in Drosophila males and some female ... of the chromosomal copy number over generations and recombination between homologous chromosomes is a hallmark of meiotic cell ... of the chromosomal copy number over generations and recombination between homologous chromosomes is a hallmark of meiotic cell ...
  • This report examines the roles of the PAH domains in mediating EMG repression during mitotic cell division and following meiotic induction. (asm.org)
  • Unlike mitotic repression, reestablishing EMG repression following transient meiotic induction requires PAH3 and PAH4. (asm.org)
  • Using a CRISPR-induced nonsense allele of sws-1 , we show that sws-1 promotes HR in mitotic and meiotic nuclei. (genetics.org)
  • Many aspects of cell cycle regulation are similar during proliferative mitotic growth and meiosis, but the different division pattern of meiosis requires modification of the mitotic cell cycle machinery to fit the needs of the meiotic differentiation program. (asm.org)
  • In addition, male myt1 germline cells occasionally undergo an extra mitotic division, resulting in meiotic cysts with twice the normal numbers of cells. (biologists.org)
  • Meiotic genes in budding yeast are repressed during vegetative growth but are transiently induced during specific stages of meiosis. (asm.org)
  • A prior study in budding yeast demonstrated that insertion of a Ty retrotransposon into a DSB hotspot can suppress meiotic break formation, but properties of Ty elements in their most common physiological contexts have not been addressed. (prolekare.cz)
  • Here we demonstrate that the budding yeast cyclin-dependent kinase Cdc28 directly regulates the formation of the DNA double-strand breaks that initiate recombination by phosphorylating the Mer2/Rec107 protein and thereby modulating interactions of Mer2 with other proteins required for break formation. (pubmedcentralcanada.ca)
  • Here, we show that under microtubule-destabilizing conditions, such as low temperature or the presence of the spindle-depolymerizing drug benomyl, meiotic budding yeast cells arrest in G 1 or G 2 , instead of metaphase. (asm.org)
  • We propose that the severe disruption of spermatogenesis during early prophase I in the absence of functional Atm may be partly due to altered interactions of telomeres with the nuclear matrix and distorted meiotic telomere clustering. (asm.org)
  • These mice, which exhibit low levels of expression of human BRCA2 protein in the gonads, are infertile because spermatocytes fail to progress beyond the early prophase I stage of meiosis. (embopress.org)
  • This implies that teliospores have already undergone premeiotic DNA synthesis and suggests that meiotic recombination initiates at a stage of infection before teliospores mature. (genetics.org)
  • Here we performed targeted and reversible ablation of premeiotic germ cells undergoing differentiation into oocytes in transgenic mice expressing the suicide gene, herpes simplex virus thymidine kinase ( HSVtk ), driven by the promoter of stimulated by retinoic acid gene 8 ( Stra8 ), a germ cell-specific gene activated during meiotic commitment. (nature.com)
  • Cells arrest in G 1 if microtubule perturbation occurs as they enter the meiotic cell cycle and in G 2 if cells are already undergoing premeiotic S phase. (asm.org)
  • In addition, a prolonged G 2 phase (meiotic prophase) separates premeiotic DNA replication from the first meiotic division. (asm.org)
  • The vegetative repression of a group of genes designated "early meiotic genes" (EMG) requires the recruitment of the histone deacetylase (HDAC) Rpd3p ( 13 ) and the chromatin-remodeling factor Isw2p ( 10 ) by the Ume6p DNA binding protein ( 31 ). (asm.org)
  • Ume6p binds an element termed upstream repressor sequence 1 (URS1) that is responsible for the full repression and activation of several early meiotic genes ( 3 , 5 , 37 ). (asm.org)
  • Decreased expression of these genes is likely to be responsible for halting both cell cycle progression and meiotic development. (asm.org)
  • find that EZH2 overexpression silences genes for the primary cilium, causing deciliation, Wnt pathway activation, and progression of BrafV600E- or NrasQ61N-driven melanomas, thus defining a tumor-suppressor role for cilia in cancer. (stanford.edu)
  • Differentiate between a gene and an allele, including the recognition that genes may have many alleles. (docplayer.net)
  • Contrast the mechanisms of inheritance of nuclear and organellar genetic information Explain how independent assortment of alleles during meiosis can lead to new combinations of alleles of unlinked genes. (docplayer.net)
  • Transmission/Patterns of Inheritance How can one deduce information about genes, alleles, and gene functions from analysis of genetic crosses and patterns of inheritance? (docplayer.net)
  • Design genetic crosses to provide information about genes, alleles, and gene functions. (docplayer.net)
  • Interpret the results of experiments comparing the phenotypes that result from single mutations in two different genes with the phenotype of the double mutant, contrasting epistatic and additive interactions. (docplayer.net)
  • Sin3p represses the early meiotic gene (EMG) by bridging the DNA binding protein Ume6p to the histone deacetylase Rpd3p. (asm.org)
  • Indeed, Abf1p helps stimulate transcription of the URS1-regulated meiotic gene HOP1 ( 37 ). (asm.org)
  • Lethal allele A mutant form of a gene that, in the homozygous state, is fatal. (bioscreening.net)
  • Lethal gene See: lethal allele. (bioscreening.net)
  • Partial or complete reactivation of the inactive X in females may be a step in breast and ovarian cancer progression, leading to overexpression of some tumour enhancing gene. (ubc.ca)
  • Draw a simple line diagram showing a segment of DNA from a gene and its RNA transcript, indicating which DNA strand is the template, the direction of transcription and the polarities of all DNA and RNA strands. (docplayer.net)
  • Explain how continuous traits are the result of many different gene combinations that can each contribute a varying amount to a phenotype. (docplayer.net)
  • In Saccharomyces cerevisiae , previous studies have shown crossover frequencies are reduced in the mismatch repair related mutant mlh3 Δ and enhanced in a meiotic checkpoint mutant pch2 Δ by up to twofold at specific chromosomal loci, but both mutants maintain high spore viability. (g3journal.org)
  • We utilized "bottom-up" nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) to identify histone PTMs and to determine their relative abundance in distinct stages of mouse spermatogenesis (meiotic, round spermatids, elongating/condensing spermatids, and mature sperm) and in human sperm. (biomedcentral.com)
  • Our results point towards the existence of a novel surveillance mechanism of microtubule integrity that may be particularly important during specialized cell cycles when coordination of cell cycle progression with a developmental program is necessary. (asm.org)
  • At FANCD2 promotes noninterfering COs in a MUS81-independent manner and is therefore part of an uncharted meiotic CO-promoting mechanism, in addition to those described previously. (plantcell.org)
  • Furthermore, differential binding of this protein to different human alleles suggests that this protein interacts with specific DNA sequences. (sciencemag.org)
  • Both bub-3 and san-1 (the C. elegans homolog of MAD3) deletion alleles confer hypersensitivity to ionizing radiation, consistent with the notion that the spindle assembly checkpoint pathway is required for the DNA damage response. (g3journal.org)
  • Interestingly, dve Dll double mutant clones could also induce ectopic antennae lacking the distal structures, suggesting that the Dve activity is crucial for repressing inappropriate antenna-forming potential in the vertex region. (sdbonline.org)
  • Double mutant analysis revealed that bub-3 does not act within any of the three major pathways involved in the repair of double-strand breaks. (g3journal.org)
  • The prophase I arrest in the follicle-enclosed oocyte is the result of low maturation promoting factor (MPF) activity, and resumption of meiosis upon the arrival of hormonal signals is mediated by activation of MPF. (diva-portal.org)
  • Thus the second aim of the thesis was to identify the specific Cdk that is essential for mouse oocyte meiotic maturation. (diva-portal.org)
  • We found that only Cdk1 is essential and sufficient for the oocyte meiotic maturation. (diva-portal.org)
  • Bergerat A, de Massy B, Gadelle D, Varoutas PC, Nicolas A, Forterre P (1997) An atypical topoisomerase II from archaea with implications for meiotic recombination. (springer.com)
  • Since the ATM protein has been implicated in telomere metabolism of somatic cells, we have set out to investigate the effects of Atm inactivation on meiotic telomere behavior. (asm.org)
  • I hypothesized that genetic and/or epigenetic factors associated with maternal meiotic non-disjunction, reproductive aging and endocrinological profile, or placental functioning will contribute to the etiology of RM. (ubc.ca)