The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
Solutions used to store organs and minimize tissue damage, particularly while awaiting implantation.
The process of protecting various samples of biological material.
A method of providing future reproductive opportunities before a medical treatment with known risk of loss of fertility. Typically reproductive organs or tissues (e.g., sperm, egg, embryos and ovarian or testicular tissues) are cryopreserved for future use before the medical treatment (e.g., chemotherapy, radiation) begins.
A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
A XANTHINE OXIDASE inhibitor that decreases URIC ACID production. It also acts as an antimetabolite on some simpler organisms.
Procedures or techniques used to keep food from spoiling.
Complex pharmaceutical substances, preparations, or matter derived from organisms usually obtained by biological methods or assay.
Elements of limited time intervals, contributing to particular results or situations.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Treatment of diseases with biological materials or biological response modifiers, such as the use of GENES; CELLS; TISSUES; organs; SERUM; VACCINES; and humoral agents.
Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
The chilling of a tissue or organ during decreased BLOOD perfusion or in the absence of blood supply. Cold ischemia time during ORGAN TRANSPLANTATION begins when the organ is cooled with a cold perfusion solution after ORGAN PROCUREMENT surgery, and ends after the tissue reaches physiological temperature during implantation procedures.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Liquids transforming into solids by the removal of heat.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Warfare involving the use of living organisms or their products as disease etiologic agents against people, animals, or plants.
Techniques, procedures, and therapies carried out on diseased organs in such a way to avoid complete removal of the organ and preserve the remaining organ function.
Total or partial excision of the larynx.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Biological activities and function of the whole organism in human, animal, microorgansims, and plants, and of the biosphere.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Remains, impressions, or traces of animals or plants of past geological times which have been preserved in the earth's crust.
An absence of warmth or heat or a temperature notably below an accustomed norm.
A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.
Bodies preserved either by the ancient Egyptian technique or due to chance under favorable climatic conditions.
The capacity to conceive or to induce conception. It may refer to either the male or female.
A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.
The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Substances that provide protection against the harmful effects of freezing temperatures.
Established cell cultures that have the potential to propagate indefinitely.
Use of naturally-occuring or genetically-engineered organisms to reduce or eliminate populations of pests.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
A local anesthetic of the ester type that has a slow onset and a short duration of action. It is mainly used for infiltration anesthesia, peripheral nerve block, and spinal block. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1016).
Sequential operating programs and data which instruct the functioning of a digital computer.
A benign SCHWANNOMA of the eighth cranial nerve (VESTIBULOCOCHLEAR NERVE), mostly arising from the vestibular branch (VESTIBULAR NERVE) during the fifth or sixth decade of life. Clinical manifestations include HEARING LOSS; HEADACHE; VERTIGO; TINNITUS; and FACIAL PAIN. Bilateral acoustic neuromas are associated with NEUROFIBROMATOSIS 2. (From Adams et al., Principles of Neurology, 6th ed, p673)
Computer-based representation of physical systems and phenomena such as chemical processes.
Tumors or cancer of the HYPOPHARYNX.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
Treatment process involving the injection of fluid into an organ or tissue.
The mechanical process of cooling.
The transference of a part of or an entire liver from one human or animal to another.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Centers for acquiring, characterizing, and storing organs or tissue for future use.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The study of early forms of life through fossil remains.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low temperature.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Comprehensive, methodical analysis of complex biological systems by monitoring responses to perturbations of biological processes. Large scale, computerized collection and analysis of the data are used to develop and test models of biological systems.
Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The rate dynamics in chemical or physical systems.
The relationships of groups of organisms as reflected by their genetic makeup.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Proteins prepared by recombinant DNA technology.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Acquisition, organization, and preparation of library materials for use, including selection, weeding, cataloging, classification, and preservation.
The physiological mechanisms that govern the rhythmic occurrence of certain biochemical, physiological, and behavioral phenomena.
Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Inability to reproduce after a specified period of unprotected intercourse. Reproductive sterility is permanent infertility.
Interacting DNA-encoded regulatory subsystems in the GENOME that coordinate input from activator and repressor TRANSCRIPTION FACTORS during development, cell differentiation, or in response to environmental cues. The networks function to ultimately specify expression of particular sets of GENES for specific conditions, times, or locations.
Substances that dissociate into two or more ions, to some extent, in water. Solutions of electrolytes thus conduct an electric current and can be decomposed by it (ELECTROLYSIS). (Grant & Hackh's Chemical Dictionary, 5th ed)
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The relationship between the dose of an administered drug and the response of the organism to the drug.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
A cell line derived from cultured tumor cells.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
Solutions which, upon administration, will temporarily arrest cardiac activity. They are used in the performance of heart surgery.
Abnormally low BODY TEMPERATURE that is intentionally induced in warm-blooded animals by artificial means. In humans, mild or moderate hypothermia has been used to reduce tissue damages, particularly after cardiac or spinal cord injuries and during subsequent surgeries.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A vasodilator used in angina of effort or ischemic heart disease.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
The technique of using a cryostat or freezing microtome, in which the temperature is regulated to -20 degrees Celsius, to cut ultrathin frozen sections for microscopic (usually, electron microscopic) examination.
The systematic study of the complete DNA sequences (GENOME) of organisms.
Databases devoted to knowledge about specific genes and gene products.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The transference of either one or both of the lungs from one human or animal to another.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.
Substances added to pharmaceutical preparations to protect them from chemical change or microbial action. They include ANTI-BACTERIAL AGENTS and antioxidants.
One of the protein CROSS-LINKING REAGENTS that is used as a disinfectant for sterilization of heat-sensitive equipment and as a laboratory reagent, especially as a fixative.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Cessation of ovarian function after MENARCHE but before the age of 40, without or with OVARIAN FOLLICLE depletion. It is characterized by the presence of OLIGOMENORRHEA or AMENORRHEA, elevated GONADOTROPINS, and low ESTRADIOL levels. It is a state of female HYPERGONADOTROPIC HYPOGONADISM. Etiologies include genetic defects, autoimmune processes, chemotherapy, radiation, and infections.
The ratio of radiation dosages required to produce identical change based on a formula comparing other types of radiation with that of gamma or roentgen rays.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Centers for acquiring and storing semen.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Extensive collections, reputedly complete, of facts and data garnered from material of a specialized subject area and made available for analysis and application. The collection can be automated by various contemporary methods for retrieval. The concept should be differentiated from DATABASES, BIBLIOGRAPHIC which is restricted to collections of bibliographic references.
Pathologic processes that affect patients after a surgical procedure. They may or may not be related to the disease for which the surgery was done, and they may or may not be direct results of the surgery.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Diminished or absent ability of a female to achieve conception.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Methods for determining interaction between PROTEINS.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Facilities that collect, store, and distribute tissues, e.g., cell lines, microorganisms, blood, sperm, milk, breast tissue, for use by others. Other uses may include transplantation and comparison of diseased tissues in the identification of cancer.
A tubular organ of VOICE production. It is located in the anterior neck, superior to the TRACHEA and inferior to the tongue and HYOID BONE.
The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.
The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.
Osmium. A very hard, gray, toxic, and nearly infusible metal element, atomic number 76, atomic weight 190.2, symbol Os. (From Dorland, 28th ed)
Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.
The protein complement of an organism coded for by its genome.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.
Antibodies produced by a single clone of cells.
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.
Restoration of blood supply to tissue which is ischemic due to decrease in normal blood supply. The decrease may result from any source including atherosclerotic obstruction, narrowing of the artery, or surgical clamping. It is primarily a procedure for treating infarction or other ischemia, by enabling viable ischemic tissue to recover, thus limiting further necrosis. However, it is thought that reperfusion can itself further damage the ischemic tissue, causing REPERFUSION INJURY.
Tongues of skin and subcutaneous tissue, sometimes including muscle, cut away from the underlying parts but often still attached at one end. They retain their own microvasculature which is also transferred to the new site. They are often used in plastic surgery for filling a defect in a neighboring region.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Organisms, biological agents, or biologically-derived agents used strategically for their positive or adverse effect on the physiology and/or reproductive health of other organisms.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A yellowish fossil resin, the gum of several species of coniferous trees, found in the alluvial deposits of northeastern Germany. It is used in molecular biology in the analysis of organic matter fossilized in amber.
Theoretical representations that simulate the behavior or activity of systems, processes, or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
The performance of surgical procedures with the aid of a microscope.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Removal of moisture from a substance (chemical, food, tissue, etc.).
The scientific study of past societies through artifacts, fossils, etc.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A series of steps taken in order to conduct research.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The portion of an interactive computer program that issues messages to and receives commands from a user.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Tumors or cancer of the human BREAST.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Clinical and laboratory techniques used to enhance fertility in humans and animals.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
The transference of a pancreas from one human or animal to another.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Optimisation of DNA and RNA extraction from archival formalin-fixed tissue. (1/383)

Archival, formalin-fixed, paraffin-embedded tissue is an invaluable resource for molecular genetic studies but the extraction of high quality nucleic acid may be problematic. We have optimised DNA extraction by comparing 10 protocols, including a commercially available kit and a novel method that utilises a thermal cycler. The thermal cycler and Chelex-100 extraction method yielded DNA capable of amplification by PCR from every block and 61% of sections versus 54% using microwave and Chelex-100, 15% with classical xylene-based extraction and 60% of sections using the kit. Successful RNA extraction was observed, by beta-actin amplification, in 83.7% sections for samples treated by the thermal cycler and Chelex-100 method. Thermal cycler and Chelex-100 extraction of nucleic acid is reliable, quick and inexpensive.  (+info)

The use of cryopreserved lymphocytes for longitudinal studies of immune function and enumeration of subpopulations. (2/383)

The responses of fresh and frozen lymphocytes to mitogens and antigens have been compared using samples collected on five separate occasions from one normal donor. The day-to-day variation seen with the fresh cells was eliminated by the use of frozen cells. Thawed cells from one donor collected on one occasion but studied on five separate occasions and compared to fresh cells on the same days, showed fluctuations from day to day as well, confirming that the day-to-day variation seen is due to technical and not biological phenomena. Cryopreserved cells showed a decrease in responses to specific microbial antigens, a slight shift in the PHA dose-response curve, but no significant difference in responses to Con A or PWM. The relative proportion of lymphocyte subpopulations changed with freezing and thawing. The proportion of T cells increased slightly and the proportion of B cells decreased.  (+info)

Preservation of Neisseria gonorrhoeae by the gelatin-disc method. (3/383)

Studies of Neisseria gonorrhoeae are difficult to perform because of the organism's poor survival in vitro. To solve this problem we tried to preserve the organism by a gelatin-disc method. The rate of survival and changes of variations in some biochemical properties of eight strains of N. gonorrhoeae were followed for three years. These studies proved that preservation was satisfactory with only a 1/10 reduction of the living cells. Another trial showed that the organism survived for over six months after being frozen at -20 degrees C. The colonial types, agglutination against red cells from rabbit and guinea pig, and antibiotic susceptibility to penicillin, chloramphenicol, tetracycline, kanamycin, and streptomycin did not change after three years' preservation.  (+info)

Survival of frozen mycoplasmas. (4/383)

Cooling to -70 C killed a higher percentage of Acholeplasma laidlawii and Mycoplasma mycoides var. capri cells than cooling to -20 C. However, to preserve cell viability for prolonged periods storage at -70 C was much more preferable. The percentage of cells surviving freezing could be increased by increasing the initial cell concentration or by the addition of dimethyl sulfoxide or glycerol as cryoprotective agents. In the presence of 1.5 M of any one of these agents survival rates of up to 100% could be obtained. The optimal cooling rates for maximal survival of A. laidlawii under the experimental conditions tested were 11 C/min for cooling to -20 C and about 15 C/min for cooling to -70 C. Increasing the warming rate during thawing from 0.6 to 67 C/min increased survival by 3 log. Oleic acid enrichment of A. laidlawii membrane lipids, or reduction in the cholesterol content of M. mycoides var. capri membranes, increased the percentage of organisms surviving freezing. Hence, the composition of membrane lipids appears to have a marked influence on the susceptibility of mycoplasmas to freezing injury.  (+info)

Anaerobic specimen transport device. (5/383)

A device is described and evaluated for the anaerobic transport of clinical specimens. The device limits the amount of oxygen entering with the sample to a maximum of 2%, which is rapidly removed by reacting with hydrogen in the presence of a palladium catalyst. The viability on swabs of 12 species of anaerobes, four strains of facultative anaerobes and a strain of Pseudomonas aeruginosa, was maintained during the length of the tests (24 or 48 h). The results demonstrated that this device protected even the more oxygen-sensitive clinical anaerobes from death due to oxygen exposure. This device can be used for swabs as well as for anaerobic collection and liquid and solid specimens.  (+info)

The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations. (6/383)

Aerated hydration (AH) treatments of cauliflower seeds for 12 h (12AH) and 28 h (28AH) at 20 degrees C resulted in improved or reduced storage potential of low or high vigour seeds, respectively. Seeds were stored at their initial seed moisture content (mean 5.5% mc) or at 12% mc at 10 degrees C for 12 months and at 20 degrees C for 4 months. The improved longevity of low vigour seeds was associated with increased K(i) (initial seed viability) and a reduced rate of deterioration (1/sigma) whereas the K(i) of high vigour seeds fell after 28AH and the rate of deterioration increased such that the time to lose one probit of viability decreased from 28.7 to 5.3 months at 10 degrees C and from 10.4 to 1.2 months at 20 degrees C. The improved K(i) of low vigour seeds could be explained by the reduction in the extent of deterioration after AH, as indicated by the increase in germination after cotrolled deterioration (CD), and the possible activation of metabolic repair during treatment. In contrast the reduced germination after CD of AH-treated high vigour seeds was indicative of deterioration as a result of treatment. Both high and low vigour seeds contained constitutive levels of ss-tubulin which increased during AH treatment, the increase being greater in high vigour seeds. High vigour seeds also showed an increase in the proportion of nuclear DNA present as 4C DNA, from 3% (untreated seeds) to 26% (28AH), indicative of germination advancement from the G(1) to G(2) phase of the cell cycle during treatment. This higher proportion of 4C DNA is correlated with the increased sensitivity of seeds to drying and/or storage after AH, leading to their reduced K(i) and storage potential. In contrast, there was little change in %4C in low vigour seeds. Priming in polyethylene glycol (PEG, -1.0 MPa) for 5 d or 13 d also improved the longevity of low vigour seeds stored at their initial and 12% mc at 10 degrees C for 8 months, as reflected in their laboratory and CD germination. In this case, however, the improved longevity of the low vigour seeds following 13 d priming was associated with an increase in 4C DNA from 4% (dry control) to 56% after treatment. The germination of both untreated and primed high vigour seeds remained high throughout the storage period. Increases in the rate of germination (decreased mean germination time) observed after all AH and PEG treatments were not consistently associated with an increase in the proportion of nuclei containing 4C DNA.  (+info)

Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniae in the tropics. (7/383)

OBJECTIVE: The preservation of Streptococcus pneumoniae by standard freezing methods for subsequent tests--such as serotyping and antibiotic susceptibility--is not possible or is difficult in many developing countries because of the high cost of equipment, inadequate equipment maintenance, and irregular power supply. We evaluated alternative low-cost methods, by comparing different culture media and storage temperatures. METHODS: Clinical isolates of five capsular types (1, 5, 7, 19, and 23) of S. pneumoniae were preserved in rabbit blood, sheep blood, skimmed milk, or glycerol-chocolate broth, and stored at -20 degrees C or -70 degrees C. The cultures were also preserved by lyophilization or sand desiccation, followed by storage at room temperature and 4 degrees C. The viability of the preserved cultures was determined by making serial colony counts on day 0 and after 1 week, 4 weeks, 4 months and 16 months. The viability of cultures preserved by sand desiccation and storage at 4 degrees C was also determined every 6 months for up to 68 months. FINDINGS: Irrespective of the media used, cultures maintained at -20 degrees C became nonviable by the fourth month, while those maintained at -70 degrees C were still viable at 16 months. Cultures preserved by lyophilization or sand desiccation lost their viability by the fourth month when maintained at local room temperature (30-42 degrees C), but remained viable when stored at 4 degrees C for up to 68 months. CONCLUSIONS: Our results confirm that freezing at -70 degrees C, or lyophilization and storage at 4 degrees C are the ideal methods for the preservation of S. pneumoniae. In laboratories where lyophilization is not feasible, sand desiccation and storage at 4 degrees C offers an alternative low-cost method for the long-term preservation of S. pneumoniae.  (+info)

Viability loss of neem (Azadirachta indica) seeds associated with membrane phase behaviour. (8/383)

Storage of neem (Azadirachta indica) seeds is difficult because of their sensitivity to chilling stress at moisture contents (MC) > or =10% or imbibitional stress below 10% MC. The hypothesis was tested that an elevated gel-to-liquid crystalline phase transition temperature (Tm) of membranes is responsible for this storage behaviour. To this end a spin probe technique, Fourier transform infrared microspectroscopy, and electron microscopy were used. The in situ Tm of hydrated membranes was between 10 degrees C and 15 degrees C, coinciding with the critical minimum temperature for germination. During storage, viability of fresh embryos was lost within two weeks at 5 degrees C, but remained high at 25 degrees C. The loss of viability coincided with an increased leakage of K+ from the embryos upon imbibition and with an increased proportion of cells with injured plasma membranes. Freeze-fracture replicas of plasma membranes from chilled, hydrated axes showed lateral phase separation and signs of the inverted hexagonal phase. Dehydrated embryos were sensitive to soaking in water, particularly at low temperatures, but fresh embryos were not. After soaking dry embryos at 5 degrees C (4 h) plus 1 d of further incubation at 25 degrees C, the axis cells were structurally disorganized and did not become turgid. In contrast, cells had a healthy appearance and were turgid after soaking at 35 degrees C. Imbibitional stress was associated with the loss of plasma membrane integrity in a limited number of cells, which expanded during further incubation of the embryos at 25 degrees C. It is suggested that the injuries brought about by storage or imbibition at sub-optimal temperatures in tropical seeds whose membranes have a high intrinsic Tm (10-15 degrees C), are caused by gel phase formation.  (+info)

Human serum albumin (HSA) is a mixture of human mercaptalbumin (HMA, reduced form) and nonmercaptalbumin (HNA, oxidized form). We have developed a convenient high-performance liquid chromatographic (HPLC) system for the separation of HSA into HMA and HNA, and studied the mercapt,==,nonmercapt conversion (i.e., dynamic change in the redox state) of HSA. Examination of long-term sample preservation temperature on the redox state of HSA is of fundamental importance for analysis of defense systems against oxidants in humans. The HMA fraction of HSA (f(HMA)) was markedly decreased, i.e., the redox state of HSA samples was more oxidized, when they were kept even at -20 degrees C for 170 days. Moreover, the redox states of five commercial HSA products were analyzed and the results were compared with those for normal control subjects. Surprisingly, marked decreases in f(HMA) value for all commercial HSA products were observed. ...
Many archaeological bones display a heterogeneous degradation pattern. Highly degraded bones could contain pockets of well-preserved bone, harbouring good quality DNA. This dichotomy may explain why the relationships between global bone preservation parameters such as histological integrity, bone mineral crystallinity or collagen yield, and bulk DNA preservation/amplification success rate have been found to be at best, weak to moderate. In this pilot study, we explore whether or not a more localised approach will highlight a stronger relationship between diagenetic parameters and DNA preservation. This study includes a detailed histological characterisation of bone diagenesis in sub-areas of three bone samples. Regions of the same bone, which displayed differential degrees of preservation or type of diagenesis were sampled for further analysis and both genetic (small scale Illumina MiSeq sequencing) and chemical (Fourier-transform infrared spectrometric analysis) analyses were performed. The aim ...
The effect of cryopreservation and long-term liquid nitrogen storage on peripheral blood mononuclear cell (PBMC) subsets was prospectively analyzed using monoclonal antibodies and flow cytometry. Brief cryopreservation did not significantly alter the proportion of positively stained cells for CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells. A small but statistically
A scientist lowers biological samples into a liquid nitrogen storage tank at the Cancer Research UK Cambridge Institute on December 9, 2014 in Cambridge, England. Healthy and cancerous biological... Get premium, high resolution news photos at Getty Images
Maneuver your liquid nitrogen storage dewar safely and easily with a roller base from by Brymill Cryogenic Systems, a provider of cryogenic equipment.
Background Non-invasively collected samples allow a variety of genetic studies on endangered and elusive species. However due to low amplification success and high genotyping error rates fewer samples can be identified up to the individual level. Number of PCRs needed to obtain reliable genotypes also noticeably increase. Methods We developed a quantitative PCR assay to measure and grade amplifiable nuclear DNA in feline faecal extracts. We determined DNA degradation in experimentally aged faecal samples and tested a suite of pre-PCR protocols to considerably improve DNA retrieval. Results Average DNA concentrations of Grade I, II and III extracts were 982pg/µl, 9.5pg/µl and 0.4pg/µl respectively. Nearly 10% of extracts had no amplifiable DNA. Microsatellite PCR success and allelic dropout rates were 92% and 1.5% in Grade I, 79% and 5% in Grade II, and 54% and 16% in Grade III respectively. Our results on experimentally aged faecal samples showed that ageing has a significant effect on quantity
Validity of biobank studies on hormone associated cancers depend on the extent the sample preservation is affecting the hormone measurements. We investigated the effect of long-term storage (up to 22 years) on immunoassay measurements of three groups of hormones and associated proteins: sex-steroids [estradiol, progesterone, testosterone, dihydroepiandrosterone sulphate (DHEAS), sex hormone-binding globulin (SHBG)], pregnancy-specific hormones [human chorionic gonadotropin (hCG), placental growth hormone (pGH), alpha-fetoprotein (AFP)], and insulin-like growth factor (IGF) family hormones exploiting the world largest serum bank, the Finnish Maternity Cohort (FMC). Hormones of interest were analyzed in a random sample of 154 Finnish women in the median age (29.5 years, range 25 to 34 years) of their first pregnancy with serum samples drawn during the first trimester. All hormone measurements were performed using commercial enzyme-linked- or radio-immunoassays. Storage time did not correlate with ...
PHCbi Ultra-Low-Temperature Freezers (-150 °C / -152 °C / -80 °C / -86°C) has an industry-leading combination features include refrigeration, control, alarm, monitoring and accessibility for reliable sample preservation. VIP ULT freezers (-80 °C / -86°C)
E2451-13 Standard Practice for Preserving Ignitable Liquids and Ignitable Liquid Residue Extracts from Fire Debris Samples sample preservation~ forensic science~ fire debris~ ignitable liquid residues~
For optimal microscopic analysis and sample preservation, we offer a broad range of aqueous and non-aqueous mounting media produced to the highest quality standards.
The formulation of novel therapeutic proteins is a challenging task which aims at finding formulation conditions that will minimize protein degradation during long-term storage. One particularly important and difficult-to-predict protein degradation pathway is the so-called non-native aggregation. The qualitative and quantitative prediction of the latter has been a subject of extensive research over the past two decades. An increasing body of evidence shows that the widely-used short-term biophysical techniques cannot accurately rank formulation conditions in order of their effect on the aggregation during long-term storage of some therapeutic proteins, e.g. monoclonal antibodies. Here we suggest a novel approach for the selection of formulation conditions that will suppress the formation of protein aggregates during long-term storage. We postulate that conditions (i.e. pH, buffer type, ionic strength) that reduce the isothermal aggregation of various denaturant-induced partially folded protein ...
TY - JOUR. T1 - Comparison of frozen and RNALater solid tissue storage methods for use in RNA expression microarrays. AU - Mutter, George L.. AU - Zahrieh, David. AU - Liu, Chunmei. AU - Neuberg, Donna. AU - Finkelstein, David. AU - Baker, Heather E.. AU - Warrington, Janet A.. PY - 2004/11/10. Y1 - 2004/11/10. N2 - Background: Primary human tissues are an invaluable widely used tool for discovery of gene expression patterns which characterize disease states. Tissue processing methods remain unstandardized, leading to unanswered concerns of how to best store collected tissues and maintain reproducibility between laboratories. We subdivided uterine myometrial tissue specimens and stored split aliquots using the most common tissue processing methods (fresh, frozen, RNALater) before comparing quantitative RNA expression profiles on the Affymetrix U133 human expression array. Split samples and inclusion of duplicates within each processing group allowed us to undertake a formal genome-wide analysis ...
Included in the range are 2D-coded microtubes with protective jacket, linear barcode and human readable code. Available in 48 and 96 format, FluidX polypropylene tubes designed for use at low temperatures, including vapour phase liquid nitrogen storage and can be used with either screw caps or TPE septum caps.
Dermaplaning is a form of manual exfoliation performed primarily on the face by a well trained and licensed medical aesthetician with special tools.
Human and chicken blood were snap-frozen as droplets in liquid ntirogen. Storage in the vapour phase liquid nitrogen container for nine months followed by rapid thawing resulted in approximately 10% haemolysis of the red cells. The ability of the thawed red cells to haemagglutinate viruses was unimpaired by the manipulations.. ...
once in contact with preservation media, culture plate, microscope slide or assay cartridge, almost all of the collected sample rapidly and spontaneously drains or rinses from the Flocked ...
The number of cycles will only be a few thousand on most drives. … All this means that SSDs are a great choice for day-to-day storage over HDDs, so long as performance is bigger priority than capacity, given the relatively higher price of a solid state drive. An SSD is not a good option for long-term storage, though.. ...
To solve this problem, Axygen have a range of Maxymum Recovery microtubes and tips which ensure extremely low retention of DNA and proteins through the use of a special plastic and high-quality moulds. This makes the Maxymum Recovery tubes the ideal solution for long-term storage of samples ...
TIANCHI Small Liquid Nitrogen Tanks 3 Liter LN2 Dewar Container Cryo Tank. Net Weight(KG):3.2. Gross Weight(KG):5.0. Static Power Dissipation(Kg/d):0.08. This Small Liquid Nitrogen Tanks 3 Liter is made of high-strength, special corrosion resistant aluminum alloy, light weight and easy to carry. Liquid nitrogen storage containers is well made, very good insulation, integrated molding, weld the small, solid and reliable, guarantee vacuum and prevent leakage. Liquid nitrogen container low loss, more than 5 years of vacuum quality.. Liquid nitrogen storage tank can be stored for a long biological specimens, semen, embryos, spawn, vaccines, skin, organ and other biological material. Products three guarantees service, for each customer to establish user files, and enjoy lifelong technical services. This LN2 Dewar Container is only suitable for the static storage of liquid nitrogen, not suitable for the transport of liquid nitrogen. This cryogenic container ships empty. It can be filled anywhere that ...
Understanding the advantages and suitability of different methods to measure residual moisture content in lyophilized materials-and the respective limitations-aids in selecting the most appropriate method for testing.
The use of energy crops and agricultural residues is expected to increase to fulfil the legislative demands of bio-based components in transport fuels. Ensiling methods, adapted from the feed sector, are suitable storage methods to preserve fresh crops throughout the year for, for example, biogas production. Various preservation methods, namely ensiling with and without acid addition for whole crop maize, fibre hemp and faba bean were investigated. For the drier fibre hemp, alkaline urea treatment was studied as well. These treatments were also explored as mild pretreatment methods to improve the disassembly and hydrolysis of these lignocellulosic substrates. The investigated storage treatments increased the availability of the substrates for biogas production from hemp and in most cases from whole maize but not from faba bean. Ensiling of hemp, without or with addition of formic acid, increased methane production by more than 50% compared to fresh hemp. Ensiling resulted in substantially increased
Eppendorf ULT Freezers such as the CryoCube FC660 are known for their energy efficiency, reliability, and worry-free, long-term sample preservation. They provide many of the same features as the Eppendorf Innova ULT Freezers, but are conventionally insulated by polyurethane foam for a cost-effective alternative. The polyurethane insulation combined with a highly efficient cooling system achieve and maintain ultra-low temperatures of -86 °C.. The ratio of the number of storage boxes to energy consumption is higher for chest freezers than upright models. When the chest freezer is opened, the cold air is kept inside. Additional insulated panels cover the inner chamber and increase energy saving. You can store up to 52,000 vessels in a CryoCube FC660. Each CryoCube model is delivered with its own specific certificate.. ...
General Information One of our goals is to supply you with the most accurate results in a timely fashion. You can help us accomplish this goal by correctly labeling submitted samples, storing them under conditions that optimize sample preservation and shipping them to us (under the best storage conditions) as soon as possible after collection. AHDC Clients: Please see the AHDC
Global Bone Marrow-Derived Stem Cells (BMSCS) Market By Service Type (Sample Preservation and Storage, Sample Analysis, Sample Processing, Sample Col
PI: Jim Smith, University of Portsmouth.. The formation of cataract in humans due to occupational or accidental exposure to acute ionising radiation is well documented. However, little is known about the effect of long-term chronic exposure through the life span of organisms exposed in the natural environment. A recent study of voles at Chernobyl (Lehmann et al. Scientific Reports 6; 2016), apparently found significant increases in cataract incidence at cumulative doses as low as 10 mSv. This generated significant media coverage and, if true, would represent a major challenge to current radiation protection regulation. Key flaws in this study, however, were the method of field sample preservation/preparation, and the subjective nature of the lens opacity measurement.. The purpose of the proposed study is to further develop methods for stabilisation and preparation of fish lens samples to measure any ultrastructural changes to the constituent crystallin proteins within the lens fibre cells caused ...
Liquid nitrogen tank is a kind of container used to store liquid nitrogen of low temperature. Liquid nitrogen tank has liquid nitrogen storage tank of storage type and liquid nitrogen transport tank for transport. Storage tank is mainly used for...
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We offer comprehensive, cradle-to-archive safety film preservation services and archival cold storage. (However, professional services are available even if you do not store your assets within our vaults.). In addition, PRO-TEK, now LAC Group, was founded by Eastman Kodak, the world leader in film innovation and manufacturing, and the only remaining maker of motion picture film in the world today. We continue to build on that heritage while we continuously innovate and modernize.. ...
Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese without English title ...
Commercial Refrigeration Qld have developed a strong reputation in the Design, installation, repairs and maintenance of commercial refrigeration in Brisbane.. With over 100 years combined experience we have built our reputation on meeting the needs of small and large companies within any type of cold storage, chemical, food manufacturing, and distribution industries.. With a dedication to professionalism, compliance and safety, our commercial refrigeration repair and installation services are a great fit for any business that needs a high-performing Commercial Refrigeration Company. We understand that you may have a few major concerns about deciding who to select to design, install, maintain, and repair your commercial refrigeration systems. We also know how frustrating and costly refrigeration system failures, breakdowns and service problems are to your business. That is why when choosing Commercial Refrigeration Qld you can be guaranteed you will get the job done right each and every ...
Learn all about storing your excess produce by freezing, drying, canning, or root cellaring. Find out how best to store each type of produce.
Currently, two main types of amniotic membrane (AM) are commercially available for in-office use: cryopreserved and dehydrated. Both types come in a variety of tissue thicknesses and sizes, depending on clinical needs.. Cryopreserved AM. Cryopreservation of AM involves slow freezing at -80°C using DMEM/glycerol preservation media to allow for slow-rate freezing without ice formation. This preservation technique retains the extracellular matrix components, such as heavy-chain hyaluronic acids, growth factors, fibronectin, and collagen, all of which promote anti-inflammatory effects and healing. The tissue is stored in a -80°C freezer and brought to room temperature when needed for use.. ProKera (BioTissue) is a cryopreserved form of AM in which the membrane is secured around a polycarbonate ring or an elastomeric band. It requires no assembly and is inserted into the eye in a manner similar to contact lens placement. This form of AM has been cleared by the FDA as a class II medical device, and ...
NOTE: The use of DMSO may not be appropriate for all cell lines (e.g. where DMSO is used to induce differentiation). In such cases an alternative such as glycerol should be used.. Following controlled-rate freezing (see above) in the presence of cryoprotectants, cell lines can be cryopreserved in a suspended state for indefinite periods provided a temperature of less than -165°C is maintained. TCCF uses ultra-low temperatures, attained by use of specialized containers (usually by immersion in liquid or vapor-phase nitrogen). The advantages and disadvantages can be discussed with the TCCF director.. Our liquid nitrogen storage vessels are equipped with alarms system. TCCF personnel ensure liquid nitrogen delivery in a timely fashion. It is highly recommended that valuable cell stocks should be backed up by storage at a second site.. ...
Saliva contains components of both the mucosal and systemic immune systems. Variable flow rates, immunoglobulin proteases, and variation in collection and storage methods all introduce differences in the estimated concentrations of antibodies. We evaluated the effect of four collection methods and three storage protocols on the concentrations of immunoglobulin A (IgA) antibodies to pneumococcal capsular antigens 1, 5, 6B, and 14 and to pneumococcal surface adhesin A (PsaA) in saliva. Specimens were collected from 30 healthy Kenyan adults by collecting drool, by pipette suction, and with two commercial kits, OraSure and Oracol. Aliquots from each specimen were snap-frozen with glycerol in liquid nitrogen or stored for 4 to 8 h at +4 degrees C either with or without the addition of protease enzyme inhibitors prior to storage at -70 degrees C. Anticapsular IgA concentrations were not significantly different with different collection methods, but snap-freezing the specimens in liquid nitrogen led to
EQUIPMENTS Well equipped with the state of the art technology, the fleet of instruments includes Real Time PCR, Class IIB2 and Class II A2 Biosafety Cabinets, high Speed centrifuge (Refrigerated with sealed rotor, Minus 40 Freezer, Cytovision software for Karyotyping and Fluorescent Imaging, Fluorescent Microscope, GeXP Genetic Analysis System, Liquid Nitrogen Storage, Inverted Microscope, CO2 Incubator. ...
Rinse and hang the nylon sprout bag on a hook or knob, arcoxia usa agonizedly rinse often, and watch the sprouts grow? Medications, drugs, and? You really have to keep up with gentle, clomid mioma manual exfoliation to keep the flaking at bay? Ces troubles surviennent le plus souvent au cours des toutes premières semaines de traitement. Cialis Professional has more strength than normal Cialis and acts faster than normal Cialis? Systemic and inhaled corticosteroids are known to increase blood glucose and worsen glycemic control in patients taking antidiabetic agents. Tricor belong to a group of medications known as fibrates, nolvadex vs clomid side effects while simvastatin belongs to a group of medications commonly referred to as the statins. Toch lees je op een dag wat af: sociale media, whats app berichten, instructies, e-mails, het laatste nieuws enz! A thorough review of these studies was completed! After surgery, most patients need to be on thyroid hormone for the rest of their life (see ...
|p|Ideal for manual exfoliation of the epidermis. Swann Mortan blades feature an open edge grinding technique that retains sharpness. |/p||ul||li|Butter blade style|/li||li|Stainless steel blade|/li||/ul|
Heatcraft Worldwide Refrigeration provides climate-control solutions for commercial refrigeration and industrial applications in more than 70 countries. We manufacture unit coolers, condensers, compressorized racks, condensing units, refrigeration systems and supermarket display cases through six market-leading brands, including Bohn, Larkin, Climate Control, Chandler, Kysor/Warren and InterLink. Heatcraft Worldwide Refrigeration is a business segment of Lennox International Inc., a leading global provider of climate-control solutions ...
Heatcraft Worldwide Refrigeration provides climate-control solutions for commercial refrigeration and industrial applications in more than 70 countries. We manufacture unit coolers, condensers, compressorized racks, condensing units, refrigeration systems and supermarket display cases through six market-leading brands, including Bohn, Larkin, Climate Control, Chandler, Kysor/Warren and InterLink. Heatcraft Worldwide Refrigeration is a business segment of Lennox International Inc., a leading global provider of climate-control solutions ...
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I think I may have hit upon a good idea for preserving mushrooms over long periods of time. This in in the absence of having a proper vacuum packing capability. After the shrooms are dry, I wrap
The 49th Industrial Refrigeration Workshop is a one-week intensive short course on industrial refrigeration as practiced in food freezing, refrigeration, and in other low temperature applications.  A systematic study of the technical background, the practice, and the latest developments in low-temperature refrigeration.
Petrel Enterprise and Smeva provide custom top quality commercial refrigeration products to a global clientele since 1920. We manufacture custom refrigerators, walk-ins, coolers & freezers for commercial use. Call us when you need quality and fair pricing.
An administrative procedure should ensure that controls by the inspection systems are carried out regularly proportionate to the degree of risk, where non-compliance is suspected and in a coordinated manner between different authorities (if several exist).. Control should also cover, as appropriate, the establishment, installations, means of transport, equipment and material; raw materials and ingredients for preparation and production of food stuffs; semi-finished and finished products; cleaning and maintenance products; processes for manufacture or processing of foodstuffs; preservation methods; labelling integrity and claims etc. Formal documentation of the export control programmes is also necessary.. 3.4 Decision criteria and action. Control programmes should target the appropriate stages of operation depending on specific objectives. The frequency and intensity of controls should be designed to take into account the risk as well as the reliability of controls already carried out by those ...
Lacto-fermentation is an ancient preservation method that greatly increases the vitamins in vegetables and other foods, and also makes the minerals more available. The food is preserved for a long time by the lactic acid produced during the fermentation process. Many fruits, vegetables and dairy products can be fermented. Fermeted foods are bursting with enzymes and good bacteria and are very beneficial for … Read More. ...
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You can vary or withdraw consent at any time, either before treatment or before the eggs are used in research or training. If this happens, your eggs will not be used.. How long can my eggs be stored for?. When you store eggs, make sure you understand the limits on the storage time and keep in contact with the clinic:. The standard storage period for eggs is normally 10 years. This period can be exceeded only in certain circumstances. Your clinician will be able to explain whether you can do this, and for how long you may be able to store your eggs. The maximum storage period is 55 years. You must let the clinic know if you change address or contact details. This is so that they will be able to contact you when the storage period is coming to an end. If they cannot contact you when the storage period ends, they may take your eggs out of storage and discard them.. What is my chance of having a baby with frozen eggs?. Since the process is a relatively new technique (the UKs first birth from a ...
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Preservation of glucose-stimulated insulin secretion". The Journal of Biological Chemistry. 281 (31): 22342-51. doi:10.1074/jbc ... The Journal of Biological Chemistry. 283 (3): 1670-8. doi:10.1074/jbc.M706540200. PMID 18042547. Jin ES, Park BH, Sherry AD, ... The Journal of Biological Chemistry. 267 (13): 8903-8. PMID 1577728. Agius L, Tosh D, Peak M (January 1993). "The contribution ... The Journal of Biological Chemistry. 279 (26): 27263-71. doi:10.1074/jbc.M401167200. PMID 15073188. Jin ES, Jones JG, Merritt M ...
41 (1). Bunzel, H.H. (1910). "The Mechanism of the Oxidation of Glucose by Bromine" (PDF). Journal of Biological Chemistry. 7 ( ... Journal of Food Processing and Preservation. ... Part I." Journal of Biological Chemistry. 75 (22): 417-422. May ... Part II". Journal of Biological Chemistry. 77 (1): 185-195. "All Chemistry". Time magazine. May 13, 1929. Retrieved 2010-07-12 ...
"Hypertexts for biological sciences. Colorado State University. Retrieved 29 April 2018.. *^ Tortora, Gerard J.; Funke, Berdell ... Hui, Y. H. (2004). Handbook of vegetable preservation and processing. New York: M. Dekker. p. 180. ISBN 978-0-8247-4301-7. . ... "Proceedings of the Royal Society B: Biological Sciences. 274 (1629): 3069-3077. doi:10.1098/rspb.2007.1067. PMC 2293941. PMID ... Biological role[edit]. Along with photosynthesis and aerobic respiration, fermentation is a way of extracting energy from ...
Tang, C. M. (2002). Osteno: Jurassic Preservation. Exceptional Fossil Preservation: A Unique View on the Evolution of Marine ... B, Biological Sciences, 311(1148), 171-180. Pinna, G. (2000). Die Fossillagerstätte im Sinemurium (Lias) von Osteno, Italien. ... It is known due to the exquisite preservation observed on the Outcrop on Osteno, where several kinds of marine biota have been ... The high local variety of fossils found can be due to unique conditions of preservation, where phosphatized soft tissues have ...
The preservation and valuation of biological resources. Univ. of Wash. Press, Seattle. 1988 The silly war: religion vs. science ... 1977 Aspects of biological exploitation. Quart. Rev. Biol. 52:371-380, reprinted from D. Wollkind (ed.), Proc. Wash. State Univ ... 1987 Habitat selection as source of biological diversity. Evolutionary Ecology 1:315-330. 1987 Editor's coda: central themes of ... Kellogg Biological Station: Eminent Ecologist (1992) Ben-Gurion University of the Negev, Israel: Jacob Blaustein Scholar (1992 ...
Exceptional preservation in the Jurassic of Osteno. Philosophical Transactions of the Royal Society of London. B, Biological ... This formation is known for its good preservation, with fossils of annelids, fishes, and plants. Arduini, P., Pinna, G., & ...
He established strong programs in biological preservation. From December 20, 1987 he served as Hawaii Volcanoes National Park ...
"Organization of Biological Field Stations". Organization of Biological Field Stations. "State of Florida granted $200,000". ... Metropoliton Dade County Historic Preservation Board. "The Richmond Era" The Charles Deering Estate Historict S.W. 167 Street ... It is also part of the Organization of Biological Field Stations through its collaboration with Florida International ... Metropoliton Dade County Historic Preservation Board. "The Deering Estate" The Charles Deering Estate Historict S.W. 167 Street ...
Sexing skeletons is based on the observation that biological males and biological females differ most in the skull and pelvis; ... "Preservation of Isotopic Signals (13c, 15n) in Pleistocene Mammals." Biogeochemical Approaches to Paleodietary Analysis. Ed. ... Cambridge Studies in Biological and Evolutionary Anthropology: Cambridge University Press, 2008. Mays, Simon. The Archaeology ... Cambridge Studies in Biological and Evolutionary Anthropology: Cambridge University Press, 2008. 273-303. Buikstra, Jane E. " ...
2008). 'Metabolic Management - Organ Procurement and Preservation For Transplantation. New York: Landes Bioscience Springer. ... Haynes RH (1988). Biological context of DNA repair. In: Friedberg EC & Hanawalt PC editors, Mechanisms and Consequences of DNA ... ATP (adenosine triphosphate) depletion is a common biological alteration that occurs with cellular injury. This change can ... Amongst other causes, this can be due to physical, chemical, infectious, biological, nutritional or immunological factors. Cell ...
"Biological Research Institute at the Zoological Society of San Diego". International Zoo Yearbook. 3 (1): 126-127. 2008-06-28. ... African Preservation Program APP (African Association of Zoological Gardens and Aquaria PAAZAB) Japan: Conservation activities ... Robert, Alexandre (2009). "Captive breeding genetics and reintroduction success". Biological Conservation. 142 (12): 2915-22. ... Biological Conservation. 115: 71-77. doi:10.1016/s0006-3207(03)00095-8. Beck BB, Kleiman DG, Dietz JM, Castro I, Carvalho C, ...
When considering any zoonosis, one must understand the social, ecological, and biological contributions that may be ... "Little Red Flying Fox (Pteropus scapulatus)". Wildlife Preservation Society of Queensland. Retrieved 21 May 2014. Plowright RK ... Biological Sciences. 275 (1636): 861-869. doi:10.1098/rspb.2007.1260. PMC 2596896. PMID 18198149. Smith, C; et al. (17 June ...
Varisco, Daniel Martin; Ross, James Perran; Milroy, Anthony (1992). Biological diversity assessment of the Republic of Yemen. ... International Council for Bird Preservation. p. 14. Retrieved 1 October 2011. MEED. Economic East Economic Digest, ltd. 1989. p ...
Schweitzer has also discovered that iron particles may play a part in the preservation of soft tissue over geologic time. On ... Biological Sciences. 272 (1565): 775-784. doi:10.1098/rspb.2004.2876. PMC 1599869. PMID 15888409. Perlman, David (April 13, ... Schweitzer, Mary H.; Cano, R. J.; Horner, J. R. (Sep 7, 1994). "Multiple Lines of Evidence for the Preservation of Collagen and ... Schweitzer, Mary H.; Chiappe, L; Garrido, A.C; Lowenstein, J.M; Pincus, S.H (April 22, 2005). "Molecular preservation in Late ...
Randall, John M. (1996). "Weed Control for the Preservation of Biological Diversity". Weed Technology. 10 (2): 370-383. doi: ... The size and shape of the park should allow adjustment in the Northwest and South for biological adjustment. The park is a ... The biggest threat from invasive weeds is the degradation of biological diversity and is therefore a major environmental threat ...
Pinna, G. (1985). "Exceptional Preservation in the Jurassic of Osteno". Philosophical Transactions of the Royal Society B: ... Biological Sciences. 311 (1148): 171-180. doi:10.1098/rstb.1985.0149. JSTOR 2396981. Poinar, G. O. (2011). The Evolutionary ...
from the middle Cambrian (Series 3, Stage 5) Spence Shale of Utah: its biological affinities and taphonomy". Journal of ... 2017). A study on the preservation of skin and keratinous integumentary structures in tetrapod fossils through time is ... Chad M. Eliason; Leah Hudson; Taylor Watts; Hector Garza; Julia A. Clarke (2017). "Exceptional preservation and the fossil ... Jérémy Tissier; Jean-Claude Rage; Michel Laurin (2017). "Exceptional soft tissues preservation in a mummified frog-eating ...
a b c Potato storage, value Preservation: Kohli, Pawanexh (2009). "Potato storage and value Preservation: The Basics" (PDF). ... GMO Safety: Genetic engineering on potatoes Biological safety research projects and results ... a b Sivasankar, B. (2002). Food Processing and Preservation. PHI Learning Pvt. Ltd. pp. 175-77. ISBN 81-203-2086-7 ... Potato storage and value Preservation: Pawanexh Kohli, CrossTree techno-visors.. *Potato, in Cyclopedia of American Agriculture ...
There are several components that are used to determine this, including biological status, which looks at the quantity and ... "Non-Native Species". Ouse Angling Preservation Society. Retrieved 25 April 2018. "SSSI Citation - Lewes Brooks" (PDF). Natural ... Cite journal requires ,journal= (help) Peck 2009, p. 1. "Glossary (see Biological quality element; Chemical status; and ... "Coarse fishing". Ouse Angling Preservation Society. Retrieved 18 April 2018. "Density and Dispersal of Introduced Pumpkinseed ...
Government preservation efforts began with the passing of the Unorganized Territories Game Preservation Act of 1894, which ... "Second Chance for the Plains Bison." Biological Conservation 136 (2007): 175-184. Gates, C.C., and N.C. Larter. "Growth and ... The first bison preservation efforts in Canada included the Unorganized Territories Game Preservation Act of 1894, through ... Preservation was the main focus of management up to World War II, which was accomplished by "feeding the bison, shooting ...
"Explosion at Navy Yard". Manhattan Project Heritage Preservation Association. Retrieved 9 April 2013. Hoddeson et al. 1993, p. ... Historical Studies in the Physical and Biological Sciences. 23 (2): 279-300. doi:10.2307/27757700. ISSN 0890-9997. Hoddeson et ...
Briggs DEG (1999). "Molecular taphonomy of animal and plant cuticles: selective preservation and diagenesis". Philosophical ... Transactions of the Royal Society B: Biological Sciences. 354 (1379): 7-17. doi:10.1098/rstb.1999.0356. PMC 1692454. PDB: 2ZI8​ ...
Ortega-Hernández, Javier; Lerosey-Aubril, Rudy; Pates, Stephen (2019-12-18). "Proclivity of nervous system preservation in ... Biological Reviews of the Cambridge Philosophical Society. 91 (1): 255-273. doi:10.1111/brv.12168. ISSN 1469-185X. PMID ... Biological Sciences. 286 (1908): 20191079. doi:10.1098/rspb.2019.1079. PMC 6710600. PMID 31362637. Cong, Peiyun; Daley, Allison ... Cambrian Burgess Shale-type deposits". Proceedings of the Royal Society B: Biological Sciences. 286 (1917): 20192370. doi: ...
1986) Its preservation potential is much greater than that of cutin. Briggs, D.E.G. (1999). "Molecular taphonomy of animal and ... plant cuticles: selective preservation and diagenesis". Philosophical Transactions of the Royal Society B: Biological Sciences ...
In the spirit of Le Corbusier's "Five Points of a New Architecture," DSA focuses on five core principles: Preservation of ... Biological Sciences. 365 (1554): 2809-2820. doi:10.1098/rstb.2010.0136. PMC 2935117. PMID 20713386. Hawley, Steve. " ... The Green Valley Agricultural Conservancy To provide assurances for the long-term preservation and management of the open space ... Civic agriculture Community-supported agriculture Green belt Preservation development Satterthwaite, David; McGranahan, Gordon ...
Wang, H.; Matzke-Karasz, R.; Horne, D. J.; Zhao, X.; Cao, M.; Zhang, H.; Wang, B. (2020). "Exceptional preservation of ... reproductive organs and giant sperm in Cretaceous ostracods". Proceedings of the Royal Society B: Biological Sciences. 287 ( ... morphology and preservation". Science Bulletin. 63 (4): 235-243. doi:10.1016/j.scib.2018.01.019. Xing, L.; McKellar, R. C.; ...
... exceptional preservation in Konzervat-lagerstätten. Covered within this field is the dominance of biological versus physical ... selective preservation and diagenesis". Philosophical Transactions of the Royal Society of London. Series B: Biological ... However, the preservation of "soft tissue" is more interesting, as it requires more peculiar conditions. While usually only ... Because of the very select processes that cause preservation, not all organisms have the same chance of being preserved. Any ...
... is the resistant biopolymer in the cell walls of unrelated groups of green algae, and facilitates their preservation ... Biological Sciences. 354 (1379): 7. doi:10.1098/rstb.1999.0356. PMC 1692454. v t e. ... Briggs, D. E. G. (1999). "Molecular taphonomy of animal and plant cuticles: selective preservation and diagenesis". ...
subscription required) Briggs, D. E. G. (2003). "The role of decay and mineralization in the preservation of soft-bodied ... Biological Sciences. 354 (1379): 7-17. doi:10.1098/rstb.1999.0356. PMC 1692454. Briggs, D. E. G. (2013). "A mosquito's last ... Stankiewicz, B. A. (1997). "Preservation of Chitin in 25-Million-Year-Old Fossils". Science. 276 (5318): 1541-1543. doi:10.1126 ... Briggs's research is on the taphonomy, or preservation, and evolutionary significance of the exceptionally preserved fossil ...
Briggs, DEG (29 January 1999). "Molecular taphonomy of animal and plant cuticles: selective preservation and diagenesis". ... International Journal of Biological Macromolecules. 98: 837-846. doi:10.1016/j.ijbiomac.2017.02.048. PMID 28223133. Jeffryes, C ... Philosophical Transactions of the Royal Society B: Biological Sciences. 354 (1379): 7-17. doi:10.1098/rstb.1999.0356. PMC ...
... others have expanded EPA's present authority to oversee the sales and use of pesticides with emphasis on the preservation of ... limitations when the Fish and Wildlife service express a potential adverse effect on a particular species based on a biological ... and provided consumers protection but did not address the growing issue of potential environmental damage and biological health ...
... their poor skeletal preservation is due to their restriction to high-energy environments, which tend to be erosional - ... The Biological Bulletin. 211 (3): 248-262. doi:10.2307/4134547. JSTOR 4134547. PMID 17179384.. ...
Folk medicine consists of the healing practices and ideas of body physiology and health preservation known to some in a culture ... Biomechanics is the study of the structure and function of biological systems by means of the methods of Mechanics. ... Biophysics is an interdisciplinary science that uses the methods of physics and physical chemistry to study biological systems. ... Histology is the study of the structures of biological tissues by light microscopy, electron microscopy and ...
"Progress in Clinical and Biological Research. 337 (7070): 301-7. doi:10.1136/bmj.313.7070.1450. PMC 2352989. PMID 8973234.. ... Penal Code and also the definition of air pollution as contained in the statutes dealing with the protection and preservation ... "Airborne exposure and biological monitoring of bar and restaurant workers before and after the introduction of a smoking ban". ... Penal Code and within the definition of air pollution as contained in the statutes dealing with protection and preservation of ...
... biological) material. Specifically, diagenesis "is the cumulative physical, chemical and biological environment; these ... in terms of preservation or destruction".[4][5] In order to assess the potential impact of diagenesis on archaeological or ... or biological change undergone by a sediment after its initial deposition, after its lithification. This process excludes ...
"Proceedings of the Royal Society B: Biological Sciences. 278 (1708): 1090-1097. doi:10.1098/rspb.2010.1579. PMC 3049027. PMID ... Due to the older timeframe and the soil chemistry on the continent, very little subfossil preservation evidence exists relative ... "Secretariat of the Convention on Biological Diversity. Retrieved 9 January 2017.. *^ Glowka, Lyle; Burhenne-Guilmin, Françoise ... We have driven the rate of biological extinction, the permanent loss of species, up several hundred times beyond its historical ...
Grammar-based codes like this can compress highly repetitive input extremely effectively, for instance, a biological data ... A survey on data compression methods for biological sequences. Information 7(4):(2016): 56 ...
A guide to their study, collection and preservation. Boca Raton, Florida: American Malacological Society, Universal Publishers ... All collecting of biological specimens is subject to proper collecting and export permits; frequently, specimens are returned ... Preservation fluids are continuously monitored and in many collections humidity and temperature are controlled to ensure the ... Brown, JP (2010). "The Field Museum Archaeological Metals Project: Distributed, In Situ Micro-Environments for the Preservation ...
Biological process. • negative regulation of neuron apoptotic process. • somitogenesis. • positive regulation of protein ... "Abrogation of the presenilin 1/beta-catenin interaction and preservation of the heterodimeric presenilin 1 complex following ...
Inggris) National Center for Home Food Preservation Nitrates and Nitrites.. *(Inggris) TR-495: Toxicology and Carcinogenesis ... Meulemans, A.; Delsenne, F. (1994). "Measurement of nitrite and nitrate levels in biological samples by capillary ...
... the Medina Aquarius Program is dedicated to the study and preservation of marine ecosystems worldwide and is enhancing the ...
National Center for Home Food Preservation-Home Preservation of pecans. *USDA Forest Service: Carya illinoensis ... Oklahoma Biological Survey: Carya illinoinensis *^ Bioimages: Carya fruits *^ Collingwood, G. H., Brush, W. D., & Butches, D., ...
see "Breaking the Biological Barriers to Cellulosic Ethanol" *^ Brinkman, N. et al., "Well-to-Wheels Analysis of Advanced/ ... Green diesel is produced through hydrocracking biological oil feedstocks, such as vegetable oils and animal fats.[47][48] ... A biofuel is a fuel that is produced through contemporary biological processes, such as agriculture and anaerobic digestion, ... Biogas can be recovered from mechanical biological treatment waste processing systems. Landfill gas, a less clean form of ...
Biological Conservation, 143(3), 710-721. *^ a b c d e Love, J. A. (1988). The reintroduction of the white-tailed sea eagle to ... International Council for Bird Preservation. ... Biological Conservation, 145(1), 79-85. *^ Dahl, E. L., May, R ... Biological Conservation, 4(5), 373-377. *^ Kalisńska, E., Salicki, W., & Jackowski, A. (2006). Six Trace Metals in White-Tailed ...
The Japanese conceived of an attack on the United States through the use of biological weapons specifically directed at the ... Garber Preservation, Restoration, and Storage Facility in Silver Hill, Maryland. There it remained in storage until 1989, when ...
Corrosion reactions proceed more rapidly as temperature increases.[4] Preservation of machinery in the presence of hot water ... Water accelerates corrosion of metal parts and is a favorable medium for biological growth. Dissolved minerals in natural water ...
Self-governance and preservation of indigenous territories become increasingly difficult as natural resources continue to be ... These may be attributed to the socio-cultural, biological, environmental and lifestyle changes seen in the First Nations, Inuit ... and genetic and biological factors (e.g. [[Thrifty gene hypothesis,thrifty genotype hypothesis]], [[thrifty phenotype]]) ,ref, ...
Proceedings of the Biological Society of Washington. 116 (1): 29-37.. *^ a b V.J. Syverson (2007). "Evolutionary Patterns in ... This problem has been addressed in California by the Ridley-Tree Condor Preservation Act, a bill that went into effect July 1, ... levels in golden eagles as well as turkey vultures has declined with the implementation of the Ridley-Tree Condor Preservation ...
Croddy, Eric (2002). Chemical and biological warfare: a comprehensive survey for the concerned citizen. Springer. p. 128. ISBN ... This was quite mistaken, and the application of quicklime can even promote preservation; although it can help eradicate the ...
Later, it was included in A. dorsata as a subspecies[1] based on the biological species concept, though authors applying a ... "Hive-stored pollen of honey bees: many lines of evidence are consistent with pollen preservation, not nutrient conversion" ... "Calcium-independent phospholipases A2 and their roles in biological processes and diseases". Journal of Lipid Research. 56 (9 ...
State regulation protective of fetal life after viability thus has both logical and biological justifications. If the State is ... for the preservation of the life or health of the mother. ...
... is the study of chemical processes and interactions of all biological and non-biological components of foods.[1] ... One of the keys to food preservation in most instances is reduce the amount of water or alter the water's characteristics to ... Comprising 75% of the biological world and 80% of all food intake for human consumption, the most common known human ... 2] The biological substances include such items as meat, poultry, lettuce, beer, and milk as examples. It is similar to ...
Hancock 2002, p. xx: 'While a nine century removal from India has diluted Indian biological connection to the extent that for ... Islam is the religion that these communities align themselves with and the people are recognized for their staunch preservation ...
Ereshefsky, M. (2001). The Poverty of the Linnaean Hierarchy: A Philosophical Study of Biological Taxonomy. Cambridge ... different environments are more favorable to the preservation of different types of organism or parts of organisms.[29] Further ... "Neoproterozoic - Cambrian Biological Revolutions" (PDF). Paleontological Society Papers. 10: 67-78. Archived from the original ... Main articles: Biological classification, Cladistics, Phylogenetic nomenclature, and Evolutionary taxonomy. .mw-parser-output ...
"Convention on Biological Diversity. Archived from the original on 19 December 2014. Retrieved 16 November 2014.. ... Redwood tree in northern California redwood forest, where many redwood trees are managed for preservation and longevity, rather ... "Definitions: Indicative definitions taken from the Report of the ad hoc technical expert group on forest biological diversity" ... "Native vegetation: cost of preservation in Australia" (PDF). Australian Commodities. 12 (3): 543-48. Archived (PDF) from the ...
... aminomethane-HCl medium is essential to the preservation of the integrity of the enzymes upon lysis. EDTA aids cell hydrolysis ... The Journal of Biological Chemistry. 240 (9): 3685-3692. PMID 4284300.. ...
... more organ preservation and fewer side effects.[84] ... Biological dose units and quantities. *Wireless electronic ...
However, similar processes take place in the leavening of bread (CO2 produced by yeast activity), and in the preservation of ... Containing Papers of a Biological Character ed.). 78 (526): 369-375.. ...
Series A, Biological Sciences and Medical Sciences. 63 (6): 556-9. doi:10.1093/gerona/63.6.556. PMC 2812805. PMID 18559628.. ... "Cellular adaptation contributes to calorie restriction-induced preservation of skeletal muscle in aged rhesus monkeys". Exp ... Spindler, Stephen R. (2010). "Biological Effects of Calorie Restriction: Implications for Modification of Human Aging". The ... representing beneficial actions linked to a low-intensity biological stressor such as reduced calorie intake.[24] As a ...
Socket grafting is a commonly adopted therapy for the preservation of alveolar bone structures in combination or not with ... immediate implant placement although the biological bases lying behind this treatment modality are not fully understood and ... Postextraction Alveolar Ridge Preservation: Biological Basis and Treatments. Giorgio Pagni. ,1 Gaia Pellegrini. ,1 William V. ... Postextraction Alveolar Ridge Preservation: Biological Basis and Treatments,. International Journal of Dentistry,. vol. 2012. ...
Previous Document: Polymer cryoprotectants in the preservation of biological ultrastructure. I. Low temperature states .... ... Next Document: Polymeric cryoprotectants in the preservation of biological ultrastructure. III. Morphological aspec.... ...
Freezing has been widely recognized as the most common process for long-term preservation of perishable foods; however, ... In conclusion, supercooling preservation has great potential to maintain food quality and extend the storage life of biological ... The article "Supercooling preservation technology in food and biological samples: a review focused on electric and magnetic ... Supercooling preservation technology in food and biological samples: a review focused on electric and magnetic field ...
Your Name) thought you would like to see the Philosophical Transactions of the Royal Society B: Biological Sciences web site. ... likewise appear to enhance preservation. The most important factor in the long-term preservation of the chemistry of both ... but in many cases longer-term preservation relies on chemical changes. Selective preservation is only a partial explanation for ... Preservation of the original chemistry of arthropod cuticles is favoured by their thickness and degree of sclerotization, and ...
Formulae Group is therefore the copyright holder after publication of an article in International Journal of Biological and ...
This innovative volume is the first to address the conservation of contemporary art made from biological materials such as ... Living Matter: The Preservation of Biological Materials in Contemporary Art An International Conference Held in Mexico City, ... Eggshells, flowers, onion peels, sponge cake, dried bread, breast milk-these are just a few of the biological materials that ... role of biological materials in the creative process and presenting a wide variety of possible approaches to their preservation ...
1998 Chemical preservation of plants and insects in natural resins. Proc. R. Soc. Lond. B 265, 641-647. (doi:10.1098/rspb. ... 1997 Preservation of chitin in 25-million-year-old fossils. Science 276, 1541-1543. (doi:10.1126/science.276.5318.1541). ... Organic preservation of fossil musculature with ultracellular detail. Maria McNamara, Patrick J. Orr, Stuart L. Kearns, Luis ... 2003 The role of decay and mineralisation in the preservation of soft-bodied fossils. Ann. Rev. Earth Plan. Sci. 31, 275-301. ( ...
Chemtrails were included in the Space Preservation Act of 2001. On October 2, 2001 U.S. Representative Dennis Kucinich ... vii) chemical, biological, environmental, climate, or tectonic weapons.. (C) The term `exotic weapons systems includes weapons ... My research interests include biological and chemical warfare policies, and I have written in particular about testing done in ... I appreciate your invitation, Senator Rockefeller, to testify about experiments involving simulated biological and chemical ...
Your Name) thought you would like to see the Proceedings of the Royal Society of London B: Biological Sciences web site. ... Preservation of hypericin and related polycyclic quinone pigments in fossil crinoids Message Subject (Your Name) has sent you a ... Preservation of hypericin and related polycyclic quinone pigments in fossil crinoids. Klaus Wolkenstein, Jürgen H Gross, Heinz ... Preservation of hypericin and related polycyclic quinone pigments in fossil crinoids. Klaus Wolkenstein, Jürgen H Gross, Heinz ...
Meat Spoilage Mechanisms and Preservation Techniques: A Critical Review D. Dave1 and Abdel E. Ghaly1 *1 Dalhousie University, ... How to Cite: Dave, D. & Ghaly, A. E. (2011). Meat Spoilage Mechanisms and Preservation Techniques: A Critical Review. American ... Approach: A comprehensive literature review was performed on the spoliage mechanisms of meat and meat products and preservation ... Proper handling, pretreatment and preservation techniques can improve the quality of meat and meat products and increase their ...
Soil-carbon preservation through habitat constraints and biological limitations on decomposer activity ... 2008). Soil-carbon preservation through habitat constraints and biological limitations on decomposer activity. Journal of Plant ... Soil-carbon preservation through habitat constraints and biological limitations on decomposer activity ... The reviewed investigations provide new and quantitative evidence that different soil C pools underlie divergent biological ...
Florida specializes in the latest of Biological Joint Preservation, including PRP, Stem Cell and Cartilage Regeneration ... Biological Joint Preservation is a broad encompassing category that includes everything from Viscosupplementation, to Stem Cell ...
0028]Hypothermic preservation of biological tissues according to the present invention may be used to preserve biological ... Other applications of hypothermic preservation of biological tissue include the high-quality preservation of food, such as ... Patent application title: Hypothermic preservation of biological tissues and cells. Inventors: Sergey V. Sheleg Hugh L. Hixon, ... 0024]Hypothermic preservation according to the present invention preserves the biological tissue in a viable state and enables ...
A wide variety of in can preservation options are available to you, such as acidity regulators, emulsifiers, and preservatives. ... offers 21,584 in can preservation products. About 9% of these are preserved fruit, 1% are preservatives, and 1% are ... Natamycin compound biological canned sardines fish preservatives cake and bread paint preservatives ... Tags: Canned Food Preservation , Mushroom Canned Food Preservation , Export Canned Food Preservation ...
Metabolomic technologies are increasingly being applied to study biological questions in a range of different settings from ... P2-Description of storage/preservation of sample(s). * Description of storage/preservation procedure * Include details such as ... The Journal of Biological Chemistry, 280, 42508-42514.CrossRefPubMedGoogle Scholar ... S1-Description of the biological sample involved in the study1 * Taxonomic classification of organism(s). Please give details ...
Microbial Domain Biological Resource Centre HAMBI, Biodiversity Collections Research Infrastructure, HiLIFE, University of ... preservation of strain and first year of storage 275 €. Annual maintenance fee 80 € invoiced every second year 165 €. ... Microbial Domain Biological Resource Centre HAMBI. HAMBI mBRC is a part of the Biodiversity Collections Research Infrastructure ...
The Biological Evidence Preservation Handbook: Best Practices for Evidence Handlers April 23, 2013. ... The report of the Technical Working Group on Biological Evidence Preservation offers guidance for individuals involved in the ... If someone leaves blood, semen or other biological material at a crime scene, scientists can use it as DNA evidence and create ...
WQM100 Unit 5 Biological Parameters,... WQM100 Unit 5 Biological Parameters, Sampling, and Preservation ... WQM100 Unit 5 Biological Parameters, Sampling, and Preservation This unit is part of the WQM100 Water Quality course of the Red ...
Biological Principles of Bone Grafting. Learning Module. Surgical Planning and Procedures. * Additional Diagnostic ...
... and preservation and their medical, surgical, and public health applications. More than a third of this editions chapters ... and preservation and their medical, surgical, and public health applications. More than a third of this editions chapters ... microbial Microbiol Microbiology microorganisms minutes organisms patients peroxide phenol practice preparations preservation ...
Targeting lipid chaperones may hold promise for lifelong preservation of metabolic health. October 10th - Researchers have ... PhD Program in Biological Sciences in Public Health. , News and Events. PhD Program in Biological Sciences in Public Health ... Rhodes is a Ph.D. student in Biological Sciences in Public Health at Harvard T.H. Chan School of Public Health, and her thesis ... PhD candidate in Biological Sciences in Public Health Maddy Mcfarland studies Trypanosoma cruzi, a parasite that transforms its ...
... ridge preservation techniques and subsequent implant placement. This module will describe the techniques to remove a hopeless ... Ridge Preservation Techniques. Learning Module. Surgical Planning and Procedures. * Biological Principles of Bone Grafting. ... describe the biological effects of tooth loss on the dento-alveolar process ... Removing the tooth without damaging the alveolar socket can facilitate post-operative healing, ridge preservation techniques ...
Various High Quality Preservation Of Potato Chips Products from Global Preservation Of Potato Chips Suppliers and Preservation ... Tags: Epsilon Polylysine , Epsilon Polylysine For Potato Chip , Biological Epsilon Polylysine For Potato Chip , View larger ... Tags: Preservation Of Potato Chips , Food Preservation , Food Preservation Of Potato Chips , View larger image ... offers 214 preservation of potato chips products. such as free samples. There are 225 preservation of potato chips ...
METHODS OF COLLECTION, PRESERVATION AND FORWARDING OF BIOLOGICAL MATERIAL FOR DNA FINGERPRINTING. ... PRESERVATION:. · Whole Blood: Blood should be collected in sterile container containing an anticoagulant. The mostly preferred ... Before DNA test is performed on any biological sample, it is mandatory that the sample should be collected, preserved and ... For forwarding other biological samples also, the signature of minimum two witnesses is required. ...
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Country of Publication:. United States. ... Journal Article: Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions ... Title: Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions ... While preservation of microbial communities is important for commercialization and research, few studies have examined storage ...
Biological Principles of Control Selection for a Humanoid Robots Dynamic Balance Preservation.. M. Vukobratovic, H. Herr, B. ... Biological Principles of Control Selection for a Humanoid Robots Dynamic Balance Preservation. International Journal of ... Low-Cost Methodology for Skin Strain Measurement of a Flexed Biological Limb.. Lin, B., Moerman, K. M., McMahan, C. G., Pasch, ... K. A., & Herr, H. M. Low-Cost Methodology for Skin Strain Measurement of a Flexed Biological Limb. IEEE Transactions on ...
Historic preservation board says proposed apartment project inappropriate for downtown. City leaders approve $250K for study to ... "Its become a significant sub area within the field of biological anthropology, and a lot of that credit goes to Mike Crawford ... Kicking that off, in spring 2016, the journal Human Biology dedicated a special issue to the KU Laboratory of Biological ... Not only did Crawford found KUs Laboratory of Biological Anthropology, hes also a founding scholar of the subfield thats ...
Cornea Preservation Time Endothelial Keratoplasty Transplant Success Endothelial Cell Density Biological: Cornea tissue ... Cornea Preservation Time Study Group. Effect of Cornea Preservation Time on Success of Descemet Stripping Automated Endothelial ... Cornea Preservation Time Study (CPTS). The safety and scientific validity of this study is the responsibility of the study ... Writing Committee for the Cornea Preservation Time Study Group. Cornea preservation time study: methods and potential impact on ...
... of duplicate gene preservation by the DDC process. The probablility of duplicate gene preservation increases with the number of ... Preservation of Duplicate Genes by Complementary, Degenerative Mutations. Allan Force, Michael Lynch, F. Bryan Pickett, Angel ... Preservation of Duplicate Genes by Complementary, Degenerative Mutations Message Subject (Your Name) has forwarded a page to ... Preservation of Duplicate Genes by Complementary, Degenerative Mutations. Allan Force, Michael Lynch, F. Bryan Pickett, Angel ...
... Neuron. 2013 Oct 30;80(3):751-64. doi: 10.1016/j ... Biological Evolution* * Brain / anatomy & histology* * Brain / physiology* * Brain Mapping * Brain Waves* / genetics ...
  • Thank you for your interest in spreading the word on Philosophical Transactions of the Royal Society B: Biological Sciences. (
  • Message Body (Your Name) thought you would like to see the Philosophical Transactions of the Royal Society B: Biological Sciences web site. (
  • American Journal of Agricultural and Biological Sciences , 6 (4), 486-510. (
  • topic of research paper in Biological sciences. (
  • Dr. Eric May is a professor of Microbiology at the University of Portsmouth School of Biological Sciences. (
  • Methods and compositions for reducing, preventing or reversing organ damage and/or enhancing organ preservation by administration of a peroxisome-proliferator activated receptor-alpha (PPARα) agonist to the organ. (
  • 1. A method for preventing, reducing or reversing organ damage or enhancing organ preservation comprising contacting the organ with a PPARα agonist in an amount effective to prevent, reduce or reverse organ damage or enhance organ preservation. (
  • The temperature-controlled organ preservation solution includes components such as oxygen carrying agents, antioxidants, tissue damage reversing and protecting agents, carrier vehicles, diluents, nutrients, and anti-coagulating agents. (
  • There is a great demand, therefore, for instrumentation for organ preservation which is easily transportable by car or air. (
  • Storage in liquid nitrogen in the absence of cryoprotectant resulted in variable preservation of dominant microorganisms in enriched samples. (
  • High values of chemical oxygen demand (COD) and biological oxygen demand (BOD 5 ) and presence of particulate matter and oil and grease in the effluent cause depletion of dissolved oxygen, which has an adverse effect on the aquatic system and also inhibits the activity and growth of microorganisms [ 11 ]. (
  • Decay and burial in terrestrial as opposed to marine, and anoxic rather than oxygenated conditions, likewise appear to enhance preservation. (
  • Selective preservation as a result of decay resistance may explain the initial survival of organic materials in sediments, but in many cases longer-term preservation relies on chemical changes. (
  • Because they are prone to rapid decay, even complete disappearance, biological materials used in art pose a range of unique conservation challenges. (
  • For this preservation to occur, the organisms must be protected from decay. (
  • The fringelite pigments, a group of phenanthroperylene quinones discovered in purple coloured specimens of the Upper Jurassic crinoid Liliocrinus , demonstrate exceptional preservation of organic compounds in macrofossils. (
  • The Burgess Shale of British Columbia is famous for its exceptional preservation of mid-Cambrian organisms. (
  • The conventional, exceptionally preserved fossils of the Burgess Shale are supplemented by the shells of organisms which lived on, and burrowed into, the sediment before the exceptional preservation pathway was complete. (
  • With contributions by conservators, scholars, curators, and artists, Living Matter is the first publication to address broadly these provocative issues, exploring the role of biological materials in the creative process and presenting a wide variety of possible approaches to their preservation. (
  • Concomitant with an increasing number of omic experiments now being carried out, the quantity of associated experimental meta-data has reached unprecedented levels and traditional approaches to biological meta-data management are no longer adequate. (
  • We present several examples (including analysis of a new engrailed gene in zebrafish) that appear to be consistent with the DDC model, and we suggest several analytical and experimental approaches for determining whether the complementary loss of gene subfunctions or the acquisition of novel functions are likely to be the primary mechanisms for the preservation of gene duplicates. (
  • As preservation administrators generate new approaches to saving the scholarly resources in the nation's academic and cultural institutions, they have called for the development of a directed, shared scientific research agenda. (
  • These included: cooling, freezing and chemical preservation. (
  • The key processes in food preparation and the chemistry behind them are described in detail, including denaturation and coagulation of proteins, gelatinisation, gelation and retrogradation of starches, thickening and gelling, browning reactions, emulsification, foams and spherification, chemical, mechanical and biological leaveners and fermentation and preservation. (
  • Explore resources to help prepare for chemical, biological, radiological and nuclear hazards. (
  • This book addresses physical, chemical, and biological methods for the preservation of ancient artifacts. (
  • Microbial growth and metabolism depends on various factors including: pre-slaughter husbandry practices, age of the animal at the time of slaughtering, handling during slaughtering, evisceration and processing, temperature controls during slaughtering, processing and distribution, preservation methods, type of packaging and handling and storage by consumer. (
  • While preservation of microbial communities is important for commercialization and research, few studies have examined storage conditions ideal for preservation. (
  • The goal of this study was to evaluate the impact of preservation method on composition of microbial communities enriched on switchgrass before and after storage. (
  • His research interests include: food nanotechnology, nutraceuticals, microbial technology and the application of modern food preservation technology. (
  • there is a great need to develop, simple, sensitive, selective and inexpensive methods for the determination of copper in environmental, biological, soil, and industrial samples for continuous monitoring to establish the levels of Cu(II) in environmental and biological matrices. (
  • Now in its thoroughly revised, updated Fifth Edition, this volume is a comprehensive, practical reference on contemporary methods of disinfection, sterilization, and preservation and their medical, surgical, and public health applications. (
  • Preservation methods included cryopreservation with the cryoprotective agents DMSO and glycerol, and cryopreservation without cryoprotective agents. (
  • The novel Preservation by Vaporization (PBV) Technology described herein provides cost-effective and efficient industrial scale stabilization of proteins, viruses, bacteria, and other sensitive biologicals, thereby allowing a production of products that are not possible to be produced by existing methods. (
  • Based upon the 10th International Symposium on the Conservation of Monuments in the Mediterranean Basin, this book provides a forum for international engineers, architects, archaeologists, conservators, geologists, art historians and scientists in the fields of physics, chemistry and biology to discuss principles, methods, and solutions for the preservation of global historical artifacts. (
  • This review is focused on understanding of ice nucleation processes and introducing the applications of EF and MF for preservation of food and biological materials. (
  • Scientists and conservation managers are increasingly focusing on the biological impacts of invasive species and on devising management practices that emphasize the health of ecosystems based on measured biological processes. (
  • A method for hypothermic preservation of biological tissue for later recovery to a viable state includes flushing the biological tissue with a gas mixture of sulfur hexafluoride or xenon and oxygen. (
  • The most successful technique for the preservation of transplant organs is the hypothermic perfusion of organs with a plasma-containing solution, called perfusate, carefully adjusted to physiological requirements of the organ. (
  • Interestingly, we found that cellular sensitivity to hypothermic hyperkalaemia was lower in cold-acclimated locusts that were better able to defend V m whilst exposed to high extracellular [K + ]. Together these results demonstrate a mechanism of cold acclimation in locusts that improves survival after cold stress: increased cold tolerance is accomplished by preservation of V m through maintenance of ion homeostasis and decreased K + sensitivity. (
  • This effort should be considered within the wider context of the reporting requirements for all types of biological samples in metabolomics experiments currently being developed by the Metabolomics Society ( Standards Initiative (MSI) of which it is part (See Preface, this issue). (
  • Furthermore, a biological sample may also include a physical substrate as a component part. (
  • He teaches food processing and preservation, physical properties of foods and food ingredients. (
  • Socket grafting is a commonly adopted therapy for the preservation of alveolar bone structures in combination or not with immediate implant placement although the biological bases lying behind this treatment modality are not fully understood and often misinterpreted. (
  • Removing the tooth without damaging the alveolar socket can facilitate post-operative healing, ridge preservation techniques and subsequent implant placement. (
  • As these structures are unique to predatory and scavenging arthropods, this form of preservation is limited to - and diagnostic of - such creatures. (
  • Our results provide unequivocal evidence that high-fidelity organic preservation of extremely labile tissues is not only feasible, but likely to be common. (
  • 3. The method of claim 1 wherein biological fluid in the container comprises at least a supernatant layer and a sediment layer, and the method includes establishing flow of at least a portion of the supernatant layer or the sediment layer through the leukocyte depletion medium. (
  • 18. The method of claim 13 wherein biological fluid in the container comprises at least a supernatant layer and a sediment layer, and the method includes establishing flow of at least a portion of the supernatant layer or the sediment layer through the leukocyte depletion medium. (
  • 19. The method of claim 14, wherein the preservation solution further comprises a cell-impermeable solute. (
  • concomitant platinum‐based chemotherapy combined with RHT in patients with high‐grade bladder cancer improves local control, bladder‐preservation rate, and OS. (
  • This retrospective analysis compares different therapeutic modalities in bladder‐preservation therapy. (
  • The results of this study show that multimodal treatment consisting of maximal transurethral resection of bladder tumor followed by radiotherapy, concomitant platinum‐based chemotherapy combined with regional deep hyperthermia in patients with Ta, Tis, T1-2 bladder carcinomas improves local control, bladder‐preservation rate, and survival. (
  • Radical cystectomy remains the treatment of choice for NMIBC who have failed intravesical therapy, but there are situations when surgery is not feasible due to competing co-morbidities or a patient's desire for bladder preservation. (
  • Combination immunoradiotherapy using PD-1/PD-L1 signaling blockade could therefore offer an interesting strategy in bladder tumors, especially as an optional bladder preservation treatment for BCG unresponsive NMIBC. (
  • The method includes pressurizing the biological tissue, preferably isothermically, with the mixture to a pressure that will form clathrates inside the biological tissue at a desired storage temperature in a range of about +1° C. to about +5° C. The method includes a step of cooling the biological tissue, preferably isobarically, to the desired storage temperature. (
  • and,cooling the biological tissue to the desired storage temperature. (
  • It seems that whilst anoxia improves Burgess Shale-type preservation, it is not essential to the process. (
  • Optional steps for further cooling to no colder than about -20° C. and for depressurization are provided as well as steps for recovering the hypothermically preserved biological tissue to a viable state, preferably using a recovery gas mixture. (
  • The objective of organ perfusion is the preservation of organs in a viable state for subsequent transplantation. (
  • The reviewed investigations provide new and quantitative evidence that different soil C pools underlie divergent biological constraints of decomposition. (
  • We therefore propose to direct future research explicitly towards such biologically nonpreferred areas where decomposition rates are slow, or where decomposition is frequently interrupted, in order to assess the potential for long-term preservation of C in the soil. (
  • The method was successfully used in the determination of Cu(II) in several Standard Reference Materials (brasses, alloys, and steels) as well as in some environmental waters (portable and polluted), biological samples (human blood and urine), soil samples and solution containing both Cu(I) and Cu(II). (
  • biological and soil samples. (
  • This research work is aimed at isolating yeasts species from the soil and using them in biological treatment of textile effluent. (
  • My research interests include the ecology and management of biological soil crusts, disturbance-dependent species, the role of disturbance in ecosystem functioning and biodiversity, and remote sensing applications to biodiversity. (
  • Conducted research into the ecological roles of biological soil crusts on arid soils, and developed strategies for their restoration. (
  • Oxygen in the sediment allows decomposition to occur at a much faster rate, which decreases the quality of the preservation, but does not prevent it entirely. (
  • Natamycin, Pimaricin, food additives Elaiomycin /Biological Preservatives hot sale! (
  • Each chapter provides in-depth information on new and emerging food preservation techniques including those relating to decontamination, drying and dehydration, packaging innovations and the use of botanicals as natural preservatives for fresh animal and plant products. (
  • There is therefore no consensus as to whether the preservation of very labile non-biomineralized tissues as organic remains is possible other than via encapsulation within natural resins (amber, copal). (
  • Appropriate procedures for blood derivatives collection and urine preservation/storage that allow maintaining as much as possible the original metabolic profile of the fresh samples emerge, and are proposed as SOPs for biobanking. (
  • A comprehensive literature review was performed on the spoliage mechanisms of meat and meat products and preservation techniques. (
  • Proper handling, pretreatment and preservation techniques can improve the quality of meat and meat products and increase their shelf life. (
  • Understanding of the intrinsic factors and extrinsic factors at every meat processing stage (from preslaughtering to meat product development) is necessary before developing proper handling, pretreatment and preservation techniques for meat. (
  • It is for this reason that techniques and instruments were developed for the preservation of viable organs. (
  • Another reason for the need of preservation techniques is that often the donor of the organ may be located in a different hospital than the intended recipient. (
  • preservation of dominant organisms. (
  • The purpose of this study is to see if the length of time the donor cornea is kept in the preservation liquid before the transplant affects the likelihood of the transplant being successful. (
  • We have no reason to believe that there is any greater risk for transplant failure with either preservation time group. (
  • How chronic inflammation contributes to age-related hearing loss, both at a population level and in terms of basic biological mechanisms, and how this knowledge can be used to slow the acceleration of age-related hearing loss. (
  • In recent years, ecological studies of naturally-occurring biological control of aquatic algae have focused on viruses, which cause lysis and subsequent death of the host organism. (
  • Rachel Rivenc is head of conservation and preservation at the Getty Research Institute. (
  • Michael Crawford, University of Kansas professor of anthropology and director of KU's Laboratory of Biological Anthropology, shows some of the DNA samples the lab has collected and is using for population research on Monday, May 8, 2017. (
  • To assist with the formation of a preservation research agenda with a collaborative stance, the Commission on Preservation and Access invited seven preservation specialists to its headquarters on October 30, 1989. (
  • The meeting's informal agenda drew upon a set of correspondence and memos regarding research needs written over the past several years by members of the Policy and Planning Committee of the Preservation of Library Materials Section/Association for Library Collections and Technical Services and the Preservation Administrators Discussion Group of the American Library Association. (
  • How can the library and archive communities articulate a workable, useful research agenda for the future that builds upon current research interests and contributes directions for specific applications for preservation needs? (
  • Because preservation practice applies knowledge from a wide variety of fields, it is a difficult task to monitor all the basic and applied research underway and to determine which is most relevant for preservation needs. (
  • Previously, no biological research was carried out in tajikistan in karst regions and caves. (
  • These cells have been and still are widely used in biological research such as creation of the polio vaccine, sex hormone steroid research, and cell metabolism. (
  • To assess the applicability of latex cell coatings as an 'off-the-shelf' biocatalyst, the effect of osmoprotectants, temperature, humidity and O 2 on preservation of H 2 production in Rhodopseudomonas palustris coatings was evaluated. (
  • Low Dark Room Temperature Preservation. (
  • Bomjen et al (5,6) has shown that DNA can be extracted from biological samples which were stored at various temperatures and for different of interval of time. (
  • Effluent discharge from textile and dyestuff industries to neighboring water bodies has become an important issue primarily due to increasing environmental considerations that has become a public and scientific concern in the light of evidence of their extremely toxic effect on humans and biological ecosystem [ 8 ]. (
  • In addition, films of phyllicate (clay) minerals can grow in situ, overprinting the biological tissue. (
  • The most important factor in the long-term preservation of the chemistry of both animal and plant cuticles, however, is diagenetic alteration to an aliphatic composition. (
  • Focusing on the regulatory complexity of eukaryotic genes, we show how complementary degenerative mutations in different regulatory elements of duplicated genes can facilitate the preservation of both duplicates, thereby increasing long-term opportunities for the evolution of new gene functions. (
  • Joint preservation is critical for the long term management of knee arthritis. (
  • Fungi have been attracting a growing interest for biological treatment of waste water ingredients such as metals, inorganic nutrient, and organic compounds through biosorption, biodegradation, bioaccumulation, and enzymatic mineralization [ 6 , 7 ]. (
  • The defining preservation process is that which preserves organic film plus phyllosilicate. (
  • Despite the urgent need for alternatives to chemicals in plant protection, biological herbicides are not widely used as biofungicides and bioinsecticides. (
  • Selective preservation is only a partial explanation for the origin of kerogen. (
  • He did not publish the work while he was alive, but wrote an abstract, titled On the Origin of Species by Means of Natural Selection, or the Preservation of Favoured Races in the Struggle for Life, which he published in 1859. (
  • Barinov E.F., Changes in extra- and intracellular electrolytes and nucleic acid metabolism in the myocardium during biological heart preservation, Voprosy meditsinskoi khimii, 1982, vol: 28(5), 14-19. (
  • 1982 The Mangrum Site, Mitigation Through Excavation and Preservation. (
  • Here we provide standard reporting requirements for describing biological samples, taken from an environmental context and involved in metabolomic experiments. (
  • The scope of our effort is to identify, develop and disseminate a core set of reporting requirements necessary for the minimal description of biological samples and procedures particular to environmental metabolomic experiments. (
  • It will be an invaluable tool in understanding the biodiversity and societal consequences of climate change, species invasions, natural disasters, the spread of disease vectors and agricultural pests, and other biological issues. (
  • The duplication-degeneration-complementation (DDC) model predicts that (1) degenerative mutations in regulatory elements can increase rather than reduce the probability of duplicate gene preservation and (2) the usual mechanism of duplicate gene preservation is the partitioning of ancestral functions rather than the evolution of new functions. (
  • Natural Selection is a manuscript written by Charles Darwin, in which he presented his theory of natural selection and its role in biological evolution. (
  • 195127 9 Claims ABSTRACT OF THE DISCLOSURE This invention relates to apparatus and technique for preservation of viable organs, mainly kidneys, for purposes of transplantation. (
  • In Engineering Geology of Ancient Works, Monuments and Historical Sites: Preservation and Protection , edited by Paul G. Marinos and George C. Koukis, pp. 519-526. (
  • D/2 Biological Solution is even used to clean the outside of the White House and also recently won a Veterans Administration contract to supply cleaner for over 3.5 million headstones and another contract to clean Civil War monuments at the Chickamauga battlefield. (
  • If someone leaves blood, semen or other biological material at a crime scene, scientists can use it as DNA evidence and create a DNA profile, or genetic fingerprint of that person. (
  • To begin building a foundation of information sources for the preservation community, the Commission contacted some of the major organizations and laboratories working in the specific areas of concern identified in the October meeting: paper, adhesives, books, and mold. (
  • For example, preservation specialists working in library and archives environments have common needs for verified documentation concerning the longevity and fragility of paper, adhesives, and other materials that are used in the production of books and other paper-based collections. (
  • This program seeks to create a national resource of digital data documenting existing biological collections and to advance scientific knowledge by improving access to digitized information (including images) residing in vouchered scientific collections across the United States. (
  • We're using Lupron (leuprolide acetate-J9217) for fertility preservation in one of our patients having chemotherapy. (
  • The next generation HemiCAP(BIO) implant is providing a biological scaffold with a cell-friendly surface environment that is supported by a stable, contoured implant. (
  • Metabolomic technologies are increasingly being applied to study biological questions in a range of different settings from clinical through to environmental. (
  • The proposed VAS may help us fabricate large-size AEM with good physicochemical and biological properties, and this AEM may have potential clinical applications in patching large/massive RC tear. (
  • The technology provides a load sharing matrix that has shown to provide complete biological surface coverage in preclinical tests, said Matthias Schurhoff , MD, VP of Clinical Operations and Scientific Affairs. (
  • Low-Cost Methodology for Skin Strain Measurement of a Flexed Biological Limb. (
  • FRANKLIN, Mass. , Dec. 17, 2010 /PRNewswire/ -- Arthrosurface, Inc. ( ), the developer of less-invasive joint resurfacing systems, announced that it has performed its first biological knee procedure based on the company's proven HemiCAP® platform technology. (
  • An experimental investigation was conducted to protect plywood panels made of perishable wood species (beech and poplar) against biological attacks with low environmental impact. (
  • The purpose of this study is to determine if the 3-year graft failure rate following endothelial keratoplasty performed with donor corneas with a preservation time of 8 to 14 days is non-inferior to the failure rate when donor corneas with a preservation time of 7 or fewer days are used. (
  • ICD-9 code 909.0 for Late effect of poisoning due to drug medicinal or biological substance is a medical classification as listed by WHO under the range -LATE EFFECTS OF INJURIES, POISONINGS, TOXIC EFFECTS, AND OTHER EXTERNAL CAUSES (905-909). (