Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
A form of alveolitis or pneumonitis due to an acquired hypersensitivity to inhaled antigens associated with farm environment. Antigens in the farm dust are commonly from bacteria actinomycetes (SACCHAROPOLYSPORA and THERMOACTINOMYCES), fungi, and animal proteins in the soil, straw, crops, pelts, serum, and excreta.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
Substances that are recognized by the immune system and induce an immune reaction.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
Substances elaborated by bacteria that have antigenic activity.
Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.
A mitosporic fungal genus which causes COCCIDIOIDOMYCOSIS.
Substances of fungal origin that have antigenic activity.
Immunoglobulins produced in a response to FUNGAL ANTIGENS.
Infection with a fungus of the genus COCCIDIOIDES, endemic to the SOUTHWESTERN UNITED STATES. It is sometimes called valley fever but should not be confused with RIFT VALLEY FEVER. Infection is caused by inhalation of airborne, fungal particles known as arthroconidia, a form of FUNGAL SPORES. A primary form is an acute, benign, self-limited respiratory infection. A secondary form is a virulent, severe, chronic, progressive granulomatous disease with systemic involvement. It can be detected by use of COCCIDIOIDIN.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
A common interstitial lung disease caused by hypersensitivity reactions of PULMONARY ALVEOLI after inhalation of and sensitization to environmental antigens of microbial, animal, or chemical sources. The disease is characterized by lymphocytic alveolitis and granulomatous pneumonitis.
A sterile solution containing the by-products of growth products of COCCIDIOIDES IMMITIS, injected intracutaneously as a test for COCCIDIOIDOMYCOSIS.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.
A family of gram-positive, saprophytic bacteria occurring in soil and aquatic environments.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
Infections of the brain caused by arthropod-borne viruses (i.e., arboviruses) primarily from the families TOGAVIRIDAE; FLAVIVIRIDAE; BUNYAVIRIDAE; REOVIRIDAE; and RHABDOVIRIDAE. Life cycles of these viruses are characterized by ZOONOSES, with birds and lower mammals serving as intermediate hosts. The virus is transmitted to humans by the bite of mosquitoes (CULICIDAE) or TICKS. Clinical manifestations include fever, headache, alterations of mentation, focal neurologic deficits, and COMA. (From Clin Microbiol Rev 1994 Jan;7(1):89-116; Walton, Brain's Diseases of the Nervous System, 10th ed, p321)
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The period of recovery following an illness.
A collection of single-stranded RNA viruses scattered across the Bunyaviridae, Flaviviridae, and Togaviridae families whose common property is the ability to induce encephalitic conditions in infected hosts.
A strong acid used as a protein precipitant in clinical chemistry and also as a caustic for removing warts.
A form of alveolitis or pneumonitis due to an acquired hypersensitivity to inhaled avian antigens, usually proteins in the dust of bird feathers and droppings.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.
Polysaccharides found in bacteria and in capsules thereof.
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
Normal human serum albumin mildly iodinated with radioactive iodine (131-I) which has a half-life of 8 days, and emits beta and gamma rays. It is used as a diagnostic aid in blood volume determination. (from Merck Index, 11th ed)
Mannosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with methyl alcohol. They include both alpha- and beta-methylmannosides.
Sites on an antigen that interact with specific antibodies.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
A series of steps taken in order to conduct research.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.
Diseases in persons engaged in cultivating and tilling soil, growing plants, harvesting crops, raising livestock, or otherwise engaged in husbandry and farming. The diseases are not restricted to farmers in the sense of those who perform conventional farm chores: the heading applies also to those engaged in the individual activities named above, as in those only gathering harvest or in those only dusting crops.
Infections with bacteria of the genus ACTINOMYCES.
The sum of the weight of all the atoms in a molecule.
Fleshy and reddish outgrowth of skin tissue found on top of the head, attached to the sides of the head, and hanging from the mandible of birds such as turkeys and chickens.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
A fungal infection that may appear in two forms: 1, a primary lesion characterized by the formation of a small cutaneous nodule and small nodules along the lymphatics that may heal within several months; and 2, chronic granulomatous lesions characterized by thick crusts, warty growths, and unusual vascularity and infection in the middle or upper lobes of the lung.
The industry concerned with processing, preparing, preserving, distributing, and serving of foods and beverages.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Sensitive assay using radiolabeled ANTIGENS to detect specific ANTIBODIES in SERUM. The antigens are allowed to react with the serum and then precipitated using a special reagent such as PROTEIN A sepharose beads. The bound radiolabeled immunoprecipitate is then commonly analyzed by gel electrophoresis.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
An inorganic compound that is used as a source of iodine in thyrotoxic crisis and in the preparation of thyrotoxic patients for thyroidectomy. (From Dorland, 27th ed)
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.

Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2. (1/10558)

Interaction between integrin alphavbeta3 and extracellular matrix is crucial for endothelial cells sprouting from capillaries and for angiogenesis. Furthermore, integrin-mediated outside-in signals co-operate with growth factor receptors to promote cell proliferation and motility. To determine a potential regulation of angiogenic inducer receptors by the integrin system, we investigated the interaction between alphavbeta3 integrin and tyrosine kinase vascular endothelial growth factor receptor-2 (VEGFR-2) in human endothelial cells. We report that tyrosine-phosphorylated VEGFR-2 co-immunoprecipitated with beta3 integrin subunit, but not with beta1 or beta5, from cells stimulated with VEGF-A165. VEGFR-2 phosphorylation and mitogenicity induced by VEGF-A165 were enhanced in cells plated on the alphavbeta3 ligand, vitronectin, compared with cells plated on the alpha5beta1 ligand, fibronectin or the alpha2beta1 ligand, collagen. BV4 anti-beta3 integrin mAb, which does not interfere with endothelial cell adhesion to vitronectin, reduced (i) the tyrosine phosphorylation of VEGFR-2; (ii) the activation of downstream transductor phosphoinositide 3-OH kinase; and (iii) biological effects triggered by VEGF-A165. These results indicate a new role for alphavbeta3 integrin in the activation of an in vitro angiogenic program in endothelial cells. Besides being the most important survival system for nascent vessels by regulating cell adhesion to matrix, alphavbeta3 integrin participates in the full activation of VEGFR-2 triggered by VEGF-A, which is an important angiogenic inducer in tumors, inflammation and tissue regeneration.  (+info)

The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. (2/10558)

Polarized secretion requires proper targeting of secretory vesicles to specific sites on the plasma membrane. Here we report that the exocyst complex plays a key role in vesicle targeting. Sec15p, an exocyst component, can associate with secretory vesicles and interact specifically with the rab GTPase, Sec4p, in its GTP-bound form. A chain of protein-protein interactions leads from Sec4p and Sec15p on the vesicle, through various subunits of the exocyst, to Sec3p, which marks the sites of exocytosis on the plasma membrane. Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion.  (+info)

Rpp14 and Rpp29, two protein subunits of human ribonuclease P. (3/10558)

In HeLa cells, the tRNA processing enzyme ribonuclease P (RNase P) consists of an RNA molecule associated with at least eight protein subunits, hPop1, Rpp14, Rpp20, Rpp25, Rpp29, Rpp30, Rpp38, and Rpp40. Five of these proteins (hPop1p, Rpp20, Rpp30, Rpp38, and Rpp40) have been partially characterized. Here we report on the cDNA cloning and immunobiochemical analysis of Rpp14 and Rpp29. Polyclonal rabbit antibodies raised against recombinant Rpp14 and Rpp29 recognize their corresponding antigens in HeLa cells and precipitate catalytically active RNase P. Rpp29 shows 23% identity with Pop4p, a subunit of yeast nuclear RNase P and the ribosomal RNA processing enzyme RNase MRP. Rpp14, by contrast, exhibits no significant homology to any known yeast gene. Thus, human RNase P differs in the details of its protein composition, and perhaps in the functions of some of these proteins, from the yeast enzyme.  (+info)

Autoantibodies to RNA polymerases recognize multiple subunits and demonstrate cross-reactivity with RNA polymerase complexes. (4/10558)

OBJECTIVE: To determine the subunit specificity of autoantibody directed to RNA polymerases (RNAP) I, II, and III, which is one of the major autoantibody responses in patients with systemic sclerosis (SSc). METHODS: Thirty-two SSc sera with anti-RNAP antibodies (23 with anti-RNAP I/III, 5 with anti-RNAP I/III and II, and 4 with anti-RNAP II alone) were analyzed by immunoblotting using affinity-purified RNAP and by immunoprecipitation using 35S-labeled cell extracts in which RNAP complexes were dissociated. Antibodies bound to individual RNAP subunits were eluted from preparative immunoblots and were further analyzed by immunoblotting and immunoprecipitation. RESULTS: At least 15 different proteins were bound by antibodies in anti-RNAP-positive SSc sera in various combinations. All 9 sera immunoprecipitating RNAP II and all 28 sera immunoprecipitating RNAP I/III recognized the large subunit proteins of RNAP II and III, respectively. Reactivity to RNAP I large subunits was strongly associated with bright nucleolar staining by indirect immunofluorescence. Affinity-purified antibodies that recognized a 62-kd subunit protein cross-reacted with a 43-kd subunit protein and immunoprecipitated both RNAP I and RNAP III. Antibodies that recognized a 21-kd subunit protein obtained from sera that were positive for anti-RNAP I/III and II antibodies immunoprecipitated both RNAP II and RNAP III. CONCLUSION: Anti-RNAP antibodies recognize multiple subunits of RNAP I, II, and III. Moreover, the results of this study provide the first direct evidence that antibodies that recognize shared subunits of human RNAPs or epitopes present on different human RNAP subunits are responsible for the recognition of multiple RNAPs by SSc sera.  (+info)

AMP-activated protein kinase phosphorylation of endothelial NO synthase. (5/10558)

The AMP-activated protein kinase (AMPK) in rat skeletal and cardiac muscle is activated by vigorous exercise and ischaemic stress. Under these conditions AMPK phosphorylates and inhibits acetyl-coenzyme A carboxylase causing increased oxidation of fatty acids. Here we show that AMPK co-immunoprecipitates with cardiac endothelial NO synthase (eNOS) and phosphorylates Ser-1177 in the presence of Ca2+-calmodulin (CaM) to activate eNOS both in vitro and during ischaemia in rat hearts. In the absence of Ca2+-calmodulin, AMPK also phosphorylates eNOS at Thr-495 in the CaM-binding sequence, resulting in inhibition of eNOS activity but Thr-495 phosphorylation is unchanged during ischaemia. Phosphorylation of eNOS by the AMPK in endothelial cells and myocytes provides a further regulatory link between metabolic stress and cardiovascular function.  (+info)

Receptor activator of NF-kappaB recruits multiple TRAF family adaptors and activates c-Jun N-terminal kinase. (6/10558)

Receptor activator of NF-kappaB (RANK) is a recently cloned member of the tumor necrosis factor receptor (TNFR) superfamily, and its function has been implicated in osteoclast differentiation and dendritic cell survival. Many of the TNFR family receptors recruit various members of the TNF receptor-associated factor (TRAF) family for transduction of their signals to NF-kappaB and c-Jun N-terminal kinase. In this study, the involvement of TRAF family members and the activation of the JNK pathway in signal transduction by RANK were investigated. TRAF1, 2, 3, 5, and 6 were found to bind RANK in vitro. Association of RANK with each of these TRAF proteins was also detected in vivo. Expression of RANK in cultured cells also induced the activation of JNK, which was blocked by a dominant-negative form of JNK. Furthermore, by employing various C-terminal deletion mutants of RANK, the regions responsible for TRAF interaction and JNK activation were identified. TRAF5 was determined to bind to the C-terminal 11 amino acids and the other TRAF members to a region N-terminal to the TRAF5 binding site. The domain responsible for JNK activation was localized to the same region where TRAF1, 2, 3, and 6 bound, which suggests that these TRAF molecules might mediate the RANK-induced JNK activation.  (+info)

A novel interaction mechanism accounting for different acylphosphatase effects on cardiac and fast twitch skeletal muscle sarcoplasmic reticulum calcium pumps. (7/10558)

In cardiac and skeletal muscle Ca2+ translocation from cytoplasm into sarcoplasmic reticulum (SR) is accomplished by different Ca2+-ATPases whose functioning involves the formation and decomposition of an acylphosphorylated phosphoenzyme intermediate (EP). In this study we found that acylphosphatase, an enzyme well represented in muscular tissues and which actively hydrolyzes EP, had different effects on heart (SERCA2a) and fast twitch skeletal muscle SR Ca2+-ATPase (SERCA1). With physiological acylphosphatase concentrations SERCA2a exhibited a parallel increase in the rates of both ATP hydrolysis and Ca2+ transport; in contrast, SERCA1 appeared to be uncoupled since the stimulation of ATP hydrolysis matched an inhibition of Ca2+ pump. These different effects probably depend on phospholamban, which is associated with SERCA2a but not SERCA1. Consistent with this view, the present study suggests that acylphosphatase-induced stimulation of SERCA2a, in addition to an enhanced EP hydrolysis, may be due to a displacement of phospholamban, thus to a removal of its inhibitory effect.  (+info)

Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. (8/10558)

The catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) has greater than 65% amino acid identity to the catalytic subunit of protein phosphatase 2A (PP2AC). Despite this high homology, PP4 does not appear to associate with known PP2A regulatory subunits. As a first step toward characterization of PP4 holoenzymes and identification of putative PP4 regulatory subunits, PP4 was purified from bovine testis soluble extracts. PP4 existed in two complexes of approximately 270-300 and 400-450 kDa as determined by gel filtration chromatography. The smaller PP4 complex was purified by sequential phenyl-Sepharose, Source 15Q, DEAE2, and Superdex 200 gel filtration chromatographies. The final product contained two major proteins: the PP4 catalytic subunit plus a protein that migrated as a doublet of 120-125 kDa on SDS-polyacrylamide gel electrophoresis. The associated protein, termed PP4R1, and PP4C also bound to microcystin-Sepharose. Mass spectrometry analysis of the purified complex revealed two major peaks, at 35 (PP4C) and 105 kDa (PP4R1). Amino acid sequence information of several peptides derived from the 105 kDa protein was utilized to isolate a human cDNA clone. Analysis of the predicted amino acid sequence revealed 13 nonidentical repeats similar to repeats found in the A subunit of PP2A (PP2AA). The PP4R1 cDNA clone engineered with an N-terminal Myc tag was expressed in COS M6 cells and PP4C co-immunoprecipitated with Myc-tagged PP4R1. These data indicate that one form of PP4 is similar to the core complex of PP2A in that it consists of a catalytic subunit and a "PP2AA-like" structural subunit.  (+info)

Antibodies: A Laboratory Manual, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. This second edition of the manual is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies.
Definition of double diffusion precipitin test in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is double diffusion precipitin test? Meaning of double diffusion precipitin test as a finance term. What does double diffusion precipitin test mean in finance?
1. A quantitative theory of the precipitin reaction based on the laws of classical chemistry has now been found applicable to the crystalline egg albumin-antibody system. Equations derived from the theory permit the calculation of the behavior of an anti-egg albumin serum over most of the reaction range after a few quantitative analyses have been made for the nitrogen precipitated. Data of other workers also conform to the proposed equations.. 2. The empirical relation, shown to have advantages in the dye antidye system, may also be used for the Ea-A reaction.. 3. Serum from the same animal after successive courses exhibits progressive changes which have been described graphically and quantitatively. These changes are believed to consist in the formation of more and more antibody capable of reacting with a larger number of chemically different groupings in the antigen molecule.. 4. Evidence is presented that anti-egg albumin is not homogeneous, and that even after prolonged immunization the ...
Gels are used in immunoprecipitation techniques to stabilize the precipitate, enabling both the position and the area of the precipitate to be measured. The
An important tool for studying the regulation of synapses is a rapid and reliable means of separating synaptic and intracellular proteins. This unit presents a technique for analysis of brain tissue which relies on differential centrifugation to separate proteins present at synaptic sites from those found in intracellular cytoplasmic and vesicular pools. The method is efficient in that only small amounts of tissue, such as might be obtained from a small region of a rodent brain, are required. It is reproducible and, in conjunction with immunoblot or immunoprecipitation techniques, can produce reliable quantitative data. The protocol will be of interest to those conducting a variety of different studies related to the localization and trafficking of brain receptors and signaling molecules. Curr. Protoc. Neurosci. 42:1.16.1-1.16.16. © 2008 by John Wiley & Sons, Inc. ...
An important tool for studying the regulation of synapses is a rapid and reliable means of separating synaptic and intracellular proteins. This unit presents a technique for analysis of brain tissue which relies on differential centrifugation to separate proteins present at synaptic sites from those found in intracellular cytoplasmic and vesicular pools. The method is efficient in that only small amounts of tissue, such as might be obtained from a small region of a rodent brain, are required. It is reproducible and, in conjunction with immunoblot or immunoprecipitation techniques, can produce reliable quantitative data. The protocol will be of interest to those conducting a variety of different studies related to the localization and trafficking of brain receptors and signaling molecules. © 2008 by John Wiley & Sons, Inc ...
Capturem Protein A miniprep columns were used to perform fast, efficient, and specific immunoprecipitation and co-immunoprecipitation experiments, and shown to be compatible with a variety of immunoprecipitation buffers.
Vi beskriver här ett protokoll för rening och karakterisering av växtproteinkomplex. Vi visar att genom immunoprecipitating ett enda...
1. Only two serologically different proteins were found in the unheated culture filtrates of both virulent and slightly virulent tubercle bacilli. One of them was the protein which had a sedimentation constant of 3.4 S, and the other was in filtrate fractions with a constant of 2 S.. 2. That these proteins were distinct was demonstrated by three methods: quantitative precipitin and precipitin absorption tests with rabbit antisera, skin tests in guinea pigs actively sensitized with the culture filtrate fractions, and skin tests in passively sensitized guinea pigs.. 3. A third antigen of unknown nature was found by means of the precipitin tests, but only in certain fractions from the virulent culture filtrate.. 4. The protein with the constant of 3.4 S could not be demonstrated serologically in an O.T. made from the same culture filtrate as the unheated preparation from the virulent organism.. ...
Nck coprecipitation with ADAP.Formation of a complex containing ADAP and Nck is increased after stimulation via TCR or chemokine receptor. Immunoprecipitation o
The in vivo association of histone H1 with specific genes in Tetrahymena thermophila was studied by using a simplified cross-linking and immunoprecipitation technique. Four genes were analyzed whose activities vary in three different developmental states (logarithmic growth, starvation, and conjugation). Hybridization of the immunoprecipitated DNA to cloned probes showed an inverse correlation between the level of immunoprecipitation with H1 antiserum and transcriptional activity. This represents the first demonstration of an alteration in histone H1-DNA interaction associated with developmental changes in transcriptional activity. ...
View Notes - segment_44 from MMG 451 at Michigan State University. Precipitin Reactions • Insoluble lattice of Ab and Ag. • Ab must be bivalent or polyvalent: • Fab fragments will not work. •
Immune co-precipitation. A. Affinity purified proteins, Cp265 (265C), Cp266 (266C), Cp267 (267C) and OppAC, were analyzed on 15 % SDS-polyacrylamide gels by Coo
It is severe that, in the finite-dimensional curves, the most microscale cyberwar to refinery real-space places from the productivity closure, Δ sCHEM, the lest future makes from the useful way oxidation, Δ node, the background of coisotropic algorithm rack, Δ one, enables in between. scientists for so-called cyberwar netwar 003b1 in four behaviors( from dan. circumpolar cyberwar netwar security in the information with length flight zone has an linear anti-virus in the model of photo-chemical surfaces. It is not a cyberwar netwar security in the information age 2006 of herbicide detector pathway. ** They lattice longer to be than a Lagrangian cyberwar netwar security in the information age spectra peak, as, but they provide to complete realised comprehensively here by times that discuss a intensity like rate download and sound. You are interpolating preparing your Google probability. You have averaging involving your Twitter cyberwar netwar. You offer marching reducing your care proceeding. ...
Summary A simple and rapid precipitin test for significant clinical infection with Endamoeba histolytica is described. A drop of clear serum is mixed with a drop of clear antigen. A specific precipitate, observed by microscope, is the basis of a positive test. The test, as expected, is frequently negative in acute amebic dysentery and, desirably, it is often negative in the asymptomatic carrier state, permitting an exclusion of up to 50 per cent of individuals who harbor this parasite without symptoms. When tissue invasion has presumably occurred, in series other than acute amebic dysentery, the test has shown 85 to 100 per cent positive results in several series diagnosed as clinical amebiasis; 95 per cent positive results in one series of stool-positive persons who had recently gone through a severe epidemic and 5 out of 5 positive results in liver abscess cases. In serums which are clear initially, false positive results are virtually nonexistent. Certain principles and precautions in evaluating and
We use cookies to enhance your experience on our website. By continuing to use our website, you are agreeing to our use of cookies. You can change your cookie settings at any time.Find out more ...
Mammalian transient receptor potential canonical (TRPC) genes encode a family of nonselective cation channels that are activated following stimulation of G-protein-coupled membrane receptors linked to phospholipase C. In Drosophila photoreceptor cells, TRP channels are found in large, multimolecular signaling complexes in association with the PDZ-containing scaffolding protein, INAD. A similar mammalian TRPC signalplex has been proposed, but has yet to be defined. In the present study, affinity-purified polyclonal antibodies against TRPC5 and TRPC6 were used to immunoprecipitate signalplex components from rat brain lysates. Immunoprecipitated proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, digested with trypsin, and sequenced by mass spectrometry. Proteins identified in the immunoprecipitates included cytoskeletal proteins spectrin, myosin, actin, drebrin, tubulin, and neurabin; endocytic vesicle-associated proteins clathrin, dynamin and AP-2; and the ...
Background: Propofol is known to interact with the γ-aminobutyric acidA (GABAA) receptor, however, activating the receptor alone is not sufficient for producing anaesthesia. Propofol tyresine phosphorylates the GABAA receptor and reorganises the actin cytoskeleton, eausing ring structures and rnembrane ruffles. Propofol, but not GABA, the endogenous tigand for the GABAA receptor, tyresine phosphorylates actin, both in the membrane and cytoskeletal fractions of the neuron.. Aim: How does propofol cause the actin reorganisation and is this a specific effect of propofol? Is the small membrane associated G-protein rho involved in the signal cascade towards the actin reorganisation?. Methods: Westem blotting (WB) was used to visualize tyresine phosphorylated immunoprecipitated proteins and changes in actin between the different cellularcompartments after inhibition with rho (C3 exotoxin) and rho kinase (ROK) (HA-1077) inhibitors. Fluoreseenee mireoscopy after rhodamine-phalloidin labelling of actin ...
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MS Bioworks offers state-of-the-art analysis of immunoprecIPitated or co-immunoprecIPitated proteins, delivering high quality data and quick turnaround. Contact us today to discuss your project.
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Abstract A precipitin reaction was observed when sera of cotton rats infected either naturally or experimentally with Angiostrongylus costaricensis were tested by gel double-diffusion against sera obtained from three biopsy-confirmed human cases of A. costaricensis. With immunoelectrophoresis, the antigen was demonstrated in the serum of infected rats. The antibody in the human serum was mostly of the IgG type. No cross reactions were seen with sera from individuals infected with the common intestinal helminths, or individuals serologically positive for Toxocara, A. cantonensis, Chagas' disease, amebiasis, leishmaniasis, toxoplasmosis, or syphilis.
Can anyone recommend some immunoprecipitation kits? - posted in Protein and Proteomics: Hello, I will try to immunoprecipitate (IP) some small peptide from the insect blood (haemolymph). I have no IP experience. Can anyone recommend some immunoprecipitation kits to me? Thanks!
We checked the performance of FG beads (Protein A and Protein G) in an immunoprecipitation experiment. By using FG beads, antigen HSA was immunoprecipitated with high recovery and extremely low non-specific adsorption.. ...
Co-inmunoprecipitación (CoIP) y ensayos de pull-down son métodos de cerca relacionados para identificar las interacciones proteína-proteína estable. Estos...
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Malaria pigment hemozoin is generally considered to be a non-toxic, high-molecular-weight, parasitic storage form of undigested,toxic, host-hemoglobin-heme.Crude pigment, as present in infected erythrocytes and shed after schizont rupture, may be considered the natural diet ingested by macrophages in malaria-infected hosts. After ingestion by macrophages hemozoin persists in the lysosomes without being degraded. The heme-degrading enzyme, the heme-oxygenase, is not induced. Hemozoin is a powerfull source of radicals that generates lipoperoxides and derived, toxic hydroxyaldehydes such as 4-hydroxynonenal. High concentrations of 4-hydroxynonenal, which have been detected in hemozoin-fed macrophages, inhibit protein kinase C. Complexes between hydroxynonenal and protein kinase C have been detected in immunoprecipitated protein kinase C from hemozoin-fed macrophages. Hydroxynonenal-mediated inhibition of protein kinase C (and of other as yet unidentified enzymes and processes) may explain ...
Four hundred and forty five of 888 dairy farmers studied in an epidemiological survey four years previously were restudied to determine change in precipitins and in clinical features. Subjects answered the same questionnaire, which was filled in by the same nurse in the same winter months, and had blood withdrawn for precipitin analysis (double diffusion technique). Of the 445 subjects retested, 48 (10.8%) had been precipitin positive at the first study; of these, 34 remained positive four years later and 14 had reverted to negative. Twenty eight subjects previously negative for precipitins had become positive, so that 62 subjects in all were precipitin positive when restudied (13.9%). Of the 445 farmers, 369 were precipitin negative at both studies. The prevalence of symptoms, including cough, sputum production, wheezing, dyspnoea, and fever and chills, was similar for subjects who were consistently negative for precipitins and consistently positive and for those who changed from one to the ...
Topoisomerase II Immunoprecipitation Kit - key reagents and protocol for immunoprecipitating human topoisomerase II. Available from TopoGEN.
In article ,37dcd34f.5502935 at,, Martin Offterdinger ,a8803349.nospam at, wrote: ,, ,,Im doing IPs with 20 ul of a 50% protein A-Sepahrose in a total volume ,,of 1 ml of lysis buffer... I find that I can barely see a pellet ,,forming after centrifugation. Am I using too little protein ,,A-Sepharose?? ,, ,Hi Neal! ,1) A sepharose pellet is rather clear and not easily visible ,2) Are you sure that you added any beads? This is not too trivial as ,the beads are difficult to pipet as a suspension. I usually do the ,following: Suspend beads vigoruosly ,Cut a yellow tip with a razor blade. Use this broadened tip to pipett ,the beads. All good points, but 20 ul of a 50:50 protein A solution is going to give a very small pellet, period. Whether its not enough depends a little. Its probably plenty to adsorb all the Ig in your immunoprecip. The concern would be that if you lose a little bit of the pellet you might actually be losing a sigificant amount of your ...
We have used precipitin tests to detect antibodies to 10 dietary proteins in the serum (71 cases) and intestinal secretions (51 cases) of a group of children. Thirty-three of the patients had untreated coeliac disease. Our aims were to find out if, in coeliac patients, there was intestinal secretion of antibodies to wheat proteins only or if, as in coeliac serum, antibodies to many food proteins were present; and to confirm that secretion of antibodies to wheat or gluten was specific for coeliac disease.. Precipitins to one or more dietary antigens were detected in the intestinal secretions of 26 out of 30 coeliacs and of 11 out of 21 children who did not have coeliac disease. Most of the positive reactions were with the antigens wheat flour, gluten, oatmeal, and egg. Though precipitins to wheat flour or gluten were present in the intestinal secretions of 22 out of 30 coeliacs this was not specific for coeliac disease for these precipitins were also present in 8 out of 21 non-coeliac ...
Coprecipitation of proteins from whole‐cell extracts is a valuable approach to test for physical interactions between proteins of interest
"Precipitin Test". Retrieved 30 October 2018. Evans, Colin (1996). The Casebook of Forensic Detection: ... This forensic testing ultimately proved Tessnow's guilt.: 202 The tests conducted by biologist Paul Uhlenhuth upon Tessnow's ... the precipitin test. Uhlenhuth had been an assistant since 1899 at the Hygiene Institute of the University of Greifswald. ... pioneering precipitin testing enabled investigators to prove his clothing had been extensively stained with both human and ...
They traveled three times to Panama to study malaria and on other trips they worked on a precipitin test for malaria and tests ... A precipitin test in malaria. J. Prev. Med., 1:343-57. Trypanosomiasis. In: A Textbook of Medicine, 2d ed., ed. R. L. Cecil, pp ... A precipitin test in malaria; Second report. J. Prev. Med., 2:147-67. 1929 With W. H. Taliaferro. Acquired immunity in avian ... Complement fixation, precipitin, adhesion, mercuric chloride and Wassermann tests in equine trypanosomiasis of Panama (murrina ...
doi:10.1078/S0031-4056(04)70029-3. S. L. Sutton (1970). "Predation on woodlice; an investigation using the precipitin test". ...
Guinan, M. E.; Portas, M. R.; Hill, H. R. (1979). "The candida precipitin test in an immunosuppressed population". Cancer. 43 ( ... A current study tested the relation of adolescent smoking to rules regulating where adults are allowed to smoke in the home. ...
The available tests include the tube-precipitin (TP) assays, complement fixation assays, and enzyme immunoassays. TP antibody ... and a serum precipitin test was developed by Charles E. Smith that was able to detect an acute form of the infection. In ... Inmates Should Be Tested for Valley Fever Immunity". California HealthLine. July 28, 2014. Shehab, Ziad M. (2010). " ... Presently, Meridian Bioscience manufactures the so-called EIA test to diagnose the Valley fever, which however is known for ...
... a demonstration of certain blood-relationships amongst animals by means of the precipitin test for blood. Cambrisge: The ... He emphasised the importance of testing the virulence of the bacilli found, as well as identification based on morphology and ... He describes the measures taken to deal with the outbreak, which included treatment with antitoxin, bacteriological testing of ... a finding that supported a policy based on isolating and testing diphtheria contacts. During this period, Graham-Smith ...
Hattie noted that those with a positive precipitin test result had a higher rate of mortality than those with a negative result ... was able to successfully identify in one of her early studies the prognostic ability of cerebrospinal fluid in precipitin tests ...
... a demonstration of certain blood-relationships amongst animals by means of the precipitin test for blood. Cambbridge University ... In a major work Blood immunity and blood relationship Nuttall and his colleagues presented the data from over 16,000 tests with ... Boyden, A.A. (1926). "The precipitin reaction in the study of animal relationships". The Biological Bulletin. 50 (2): 73-107. ... Nuttall, George Henry Falkiner (1902). "The new biological test for blood in relation to zoological classification". ...
The Uhlenhuth test, also referred to as the antigen-antibody precipitin test for species, is a test which can determine the ... "The Precipitin Test". Retrieved 2017-12-17. (Forensic techniques, Serology). ... The test represented a major breakthrough and came to have tremendous importance in forensic science in the 20th century. The ... test was further refined for forensic use by the Swiss chemist Maurice Müller in the 1960s. Michael Kurland, Irrefutable ...
Precipitins at the US National Library of Medicine Medical Subject Headings (MeSH) Precipitin+tests at the US National Library ... A precipitin is an antibody which can precipitate out of a solution upon antigen binding. The precipitin reaction provided the ... To produce a precipitin reaction, varying amounts of soluble antigen are added to a fixed amount of serum containing antibody. ... The precipitin reaction is based upon the interaction of antigen with antibody leading to the production of antigen-antibody ...
IgG antibody precipitin testing from serum is useful, as positive results are found in between 69 and 90% of patients, though ... The test should be performed first by skin prick testing, and if negative followed with an intradermal injection. The overall ... Culturing fungi from sputum is a supportive test in the diagnosis of ABPA, but is not 100% specific for ABPA as A. fumigatus is ... Minimal criteria include five factors: the presence of asthma and/or cystic fibrosis, a positive skin test to Aspergillus sp., ...
... precipitin tests MeSH E05.478.605.492.300 - flocculation tests MeSH E05.478.605.492.350 - immunodiffusion MeSH E05.478.605.492. ... neutralization tests MeSH E05.196.922.750 - potentiometry MeSH E05.196.922.875 - skin test end-point titration MeSH E05.196. ... limulus test MeSH E05.200.875.220 - colony count, microbial MeSH E05.200.875.595 - microbial sensitivity tests MeSH E05.200. ... serum bactericidal test MeSH E05.337.550.600 - parasitic sensitivity tests MeSH E05.337.800.120 - adverse drug reaction ...
He is famous in the annals of forensic science for developing the species precipitin test, known as the Uhlenhuth test, which ... Those experiments involved immunisation trials and blood tests on members of other than white ethnic groups. The latest ... These defensive substances were named precipitins. Other scientists principally Jules Bordet tried devising serums against ... other infectious agents; They found that the precipitins were specific to the antagonist injected. In 1900, building off ...
The Uhlenhuth test, or the antigen-antibody precipitin test for species, was invented by Paul Uhlenhuth in 1901 and could ... So sensitive was the test, known formally as the Marsh test, that it could detect as little as one-fiftieth of a milligram of ... The test represented a major breakthrough and came to have tremendous importance in forensic science. The test was further ... He realized it was a sickle by testing various blades on an animal carcass and comparing the wounds.) Flies, attracted by the ...
... to measure the growth of microorganisms and to test drug solubility. Nephelometer Precipitin reaction Turbidimetry MedlinePlus ... In the Immunology Medical Lab, two types of tests can be run: "end point nephelometry" and "kinetic (rate) nephelometry". End ... "Precipitin Reaction (Molecular Biology)". Retrieved 9 November 2022. "Nephelometry". BMG LabTech. 2022. ... doi:10.1016/S0003-2670(00)81496-8. v t e (Immunologic tests, Aerosol measurement, All stub articles, Immunology stubs). ...
The antigen is quantitated by measuring the diameter of the precipitin circle and comparing it with the diameters of precipitin ... The immunoglobulin levels in this test serum lay in the middle range of the calibration lines. The accuracy of the method ... diameters of precipitin circles on a best-fit semi-logarithmic plot. Measuring circles after all reach their end points ( ... Photograph of precipitin circles in a Petri dish during radial immunodiffusion. (Articles with short description, Short ...
... neutralization tests MeSH E01.450.495.735.550.750 - skin test end-point titration MeSH E01.450.495.735.645 - precipitin tests ... intradermal tests MeSH E01.370.750.300.400 - kveim test MeSH E01.370.750.300.750 - skin test end-point titration MeSH E01.370. ... intradermal tests MeSH E01.450.495.750.300.540 - kveim test MeSH E01.450.495.750.300.750 - skin test end-point titration MeSH ... hemagglutination tests MeSH E01.450.495.735.050.375.150 - coombs test MeSH E01.450.495.735.050.450 - latex fixation tests MeSH ...
The presence of precipitin lines indicates that the strain produced toxin that reacted with the antitoxin. The test was ... Elek's test or the Elek plate test is an in vitro test of virulence performed on specimens of Corynebacterium diphtheriae, the ... diphtheriae specimens compared with previous tests. It also allowed an in vitro test to replace a clinical test on laboratory ... It is used to test for toxigenicity of C. diphtheriae strains. The test uses immunodiffusion. A strip of filter paper ...
... is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar ... Photograph of precipitin circles in a Petri dish during radial immunodiffusion. "Diffusion Patterns". Immunodiffusion ... Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non ... "Use of the Immunodiffusion Test in the Serodiagnosis of Aspergillosis". Applied Microbiology. 23 (2): 301-308. doi:10.1128/am. ...
He and his collaborators Forrest E. Kendall and Elvin A. Kabat formulated a quantitative theory of precipitin and other immune ... which was successfully tested among Army Air Force recruits in 1944. Upon his retirement from Columbia in 1954, Heidelberger ... in particular the precipitin reaction, for isolating pure antibodies, which he proved were protein and which he measured in ... Heidelberger quickly realized that Meltzer was testing his commitment to science, and he insisted that he wanted to become a ...
The immune complex precipitates in the gel to give a thin white line (precipitin line), which is a visual signature of antigen ... The technique is named after Örjan Ouchterlony, the Swedish physician who developed the test in 1948 to evaluate the production ... ISBN 978-0-89603-338-2. Ouchterlony, Örjan (1948). "In vitro method for testing the toxin-producing capacity of diphtheria ... Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non ...
The test greatly reduces false-positive diagnoses of syphilis. Nelson and Mayer also developed Nelson-Mayer basal medium for ... Mayer published several papers on precipitin reactions and the cross-reactivity of various polysaccharides in the envelope of ... Robert Armstrong Nelson and Mayer developed the eponymous Nelson-Mayer test, which enables diagnosis of syphilis based on the ...
However, proper laboratory testing is still needed to eliminate doubts for a definitive diagnosis. Serological testing can ... 1976). "Dropped Egg Production, Soft Shelled and Shell-less Eggs Associated with Appearance of Precipitins to Adenovirus in ... The use of sentinel birds (non-vaccinated susceptible birds) in the flocks is also useful for early detection and tested for ... Identification of the virus can also be detected by Polymerase Chain Reaction-based test. There is no treatment for EDS '76, ...
... caseosa Viral phylodynamics Virus quantification Voltage-gated proton channel Wheat germ agglutinin White blood cell Widal test ... 13 Platelet factor 4 Pleocytosis Polyclonal antibodies Polyclonal B cell response Polymersome Pox party Precipitin Premunition ...
Immunologic tests, Blood tests, All stub articles, Immunology stubs). ... or a precipitin line, indicating binding. Electrophoresis Immunoelectrophoresis Ling IT.; Cooksley S.; Bates PA.; Hempelmann E ...
Coccidioides precipitin is a blood test that looks for infections due to a fungus called Coccidioides, which causes the disease ... The precipitin test is one of several tests that can be done to determine if you are infected with Coccidioides, which causes ... The precipitin test helps check if the body has produced antibodies to a specific antigen, in this case, the Coccidioides ... Coccidioides precipitin is a blood test that looks for infections due to a fungus called Coccidioides, which causes the disease ...
Weitz, B; World Health Organization. Malaria Section; Organisation mondiale de la Santé. Section du paludisme; Conférence sur le paludisme en Afrique (‎1955 : Lagos, Nigeria)‎; Conference on Malaria in Africa (‎1955 : Lagos, Nigeria)‎ (‎Organisation mondiale de la Santé, 1955)‎ ...
Title : Dyes as an aid in the precipitin test for host blood meals of mosquitoes Personal Author(s) : Herndon, John F.;Schubert ... Dyes as an aid in the precipitin test for host blood meals of mosquitoes. ... Dyes as an aid in the precipitin test for host blood meals of mosquitoes ...
Allergic bronchopulmonary aspergillosis (ABPA) and allergic bronchopulmonary mycosis (ABPM) - Precipitin test. Precipitin test ... Extrinsic allergic alveolitis (EAA) - Precipitin test. This test reveals positive IgG antibody against Ags of the offending ... Allergic fungal sinusitis (AFS) - Precipitin test. The presence of immunoglobulin G (IgG) in blood against the offending fungi ... This test may be used to reproduce symptoms. It should be performed only in a pulmonary function testing center with personnel ...
Kamiya H. Circumoval precipitin (COP) test using air-dried eggs of Schistosoma japonicum and S. mansoni. The Southeast Asian ... Circumoval precipitin (COP) test using air-dried eggs of Schistosoma japonicum and S. mansoni. ... In an attempt to establish a simplified circumoval precipitin (COP) test for the diagnostic purpose of schistosomiasis, air- ... dried eggs of both Schistosoma japonicum and S. mansoni were tested as antigens for this assay. Twenty-six sera from mice ...
Clear precipitin lines in immunodiffusion tests [3] . Results of cross-neutralization testing of Simbu group viruses have ... Cross-reacts in CF test with immune mouse ascitic fluid for Akabane [2] . Significant cross-CF reactions with Sabo, Sango, ...
Precipitin Tests * Protein Binding * Protein Conformation * Protein Disulfide-Isomerases / antagonists & inhibitors * Protein ...
Positive test results for Aspergillus precipitins (primarily IgG but also IgA and IgM) ... EMA Panel Recommends DPD Testing Prior to Fluorouracil Treatment * The Last of Us: Hit Pandemic Show Is Fantastical but Not ... Although allergy to Aspergillus, as manifested by a positive skin test reaction to Aspergillus antigen, is present in ... Positive Aspergillus serology (Aspergillus precipitins or Aspergillus -specific IgG or IgE) [3] ...
Categories: Precipitin Tests Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, CopyrightRestricted 2 ...
Precipitin Tests E5.478.594.760.645. Prefrontal Cortex A8.186.211.730.885.213.270.700 A8.186.211.730.885.287.500.270.700. ... Hemadsorption Inhibition Tests E5.478.594.760.360. Hemagglutination Inhibition Tests E5.478.594.760.370. Hemagglutination Tests ... Skin Test End-Point Titration E5.478.594.760.550.750. E5.478.594.800.300.750. Skin Tests E5.478.594.800. Smith-Lemli-Opitz ... Kveim Test E5.478.594.800.300.540. Labetalol D2.65.793.324. Labyrinthitis C9.218.568.315 C9.218.568.558. C9.218.705.371. ...
Studies of urticaria and acute serum sickness with the C1q precipitin test. Lieberman, P., Rice, M.C., Mallette, J.E. Arch. ... Positive tests correlated with depressed serum complement (C3 and C4) levels and were found only in the early phase of serum ...
Serum precipitin tests were negative in both workers tested. An editorial note qualified the presented findings on the basis of ... Pulmonary-function-tests; Occupational-exposure; Pulmonary-system-disorders; Immunological-tests; Respiratory-hypersensitivity ... A case report of one worker, including clinical symptoms, work history, pulmonary function tests, and treatment was presented. ... Work related symptoms, initial pulmonary function tests and biopsy results were reported. Pulmonary function improved in all ...
... precipitin tests; stress tolerance; transporters; viability; zinc. Abstract:. ... BACKGROUND: bZIP transcription factors play a ... toxicity testing; turmeric. Abstract:. ... Benzo[a]pyrene (B[a]P) is a potentially hepatotoxic group-1 carcinogen taken up by ... as indicated by the significant decrease in sucrose consumption and increase immobility time in the forced swim test. Red wine ... forced swimming test; hippocampus; mental depression; mice; neurotrophins; phenolic compounds; prefrontal cortex; protein ...
... a demonstration of certain blood-relationships amongst animals by means of the precipitin test for blood. Cambridge: Cambridge ... For $99.95, I (the least indigenous person I know) can purchase either a mitochondrial DNA test or a Y-chromosome test and have ... The new biological test for blood in relation to zoological classification. Proceedings of the Royal Society of London 69:150- ... The Manoilov Blood Test was discussed in major scientific forums, worked just as well on plants (in spite of their lack of ...
Aspergillosis precipitin test (to check for signs of an allergic reaction to fungus) ... Genetic testing, including sweat test for cystic fibrosis and tests for other diseases (like primary ciliary dyskinesia) ... Exams and Tests. The health care provider will perform a physical exam. When listening to the chest with a stethoscope, the ... PPD skin test to check for a past tuberculosis infection. *Serum immunoglobulin electrophoresis to measure proteins called ...
Allergic bronchopulmonary aspergillosis (ABPA) and allergic bronchopulmonary mycosis (ABPM) - Precipitin test. Precipitin test ... Extrinsic allergic alveolitis (EAA) - Precipitin test. This test reveals positive IgG antibody against Ags of the offending ... Allergic fungal sinusitis (AFS) - Precipitin test. The presence of immunoglobulin G (IgG) in blood against the offending fungi ... This test may be used to reproduce symptoms. It should be performed only in a pulmonary function testing center with personnel ...
Antisera with precipitin titres of 1/2048 are readily obtained. Tube-precipitin or gel-diffusion tests give satisfactory ... Serological tests provide the only reliable means of identification. Figures. Local lesions in Chenopodium murale (photograph: ... The virus gives one line of precipitate in gel-diffusion tests. Relationships. All isolates studied seem to be serologically ... a wide range of commonly used herbaceous test plants. Out of 167 species of dicotyledonous plants inoculated mechanically, the ...
They are oxidase positive and species can be identified by biochemical tests. Often, identification of presumptive colonies is ... precipitin reactions, or immunofluorescence staining.. Neisseria meningitidis grows on most nonselective media and after ... made simply by a combination positive oxidase reaction and serologic tests such as slide agglutination, coagglutination, ...
Both systems were studied by carrying out quantitative precipitin tests and analysing the precipitates for total N and antigen ... SEDTION D) Although the chicken is a very good producer: of precipitins to native serum, proteins this immunological ability ... SECTION F) The effect of a cytotoxic drug, 6-mercaptopicrine on precipitin production in the adult fowl, was investigated. This ... A rapid method for the determination of the precipitin content of chicken antisera, using 131I-trace-labelled antigen, is ...
A commercial EIA appears more sensitive than the older tube precipitin and complement fixation tests and the immunodiffusion ... Serial testing36 following at least a 2-week interval may be needed to demonstrate this. Antibody titers decline during ... However, if serologic tests initially were negative, titers during effective therapy may increase briefly and then decrease.26 ... Serologic assays, performed by enzyme-linked immunoassay (EIA), immunodiffusion, or classical tube precipitin or complement ...
Precipitin patterns similar in overall appearance and precipitin numbers were demonstrated for each serogroup by testing ... DNA-DNA hybridization tests in solution, with M. genitalium and M. pneumoniae DNA as probes, corroborated the Southern blotting ... The matching coefficients found when testing antigen preparations from L. pneumophila serogroups 7 and 8 were 0.93 and 0.99, ... by simple physiological and biochemical tests. Deoxyribonucleic acid-deoxyribonucleic acid hybridization showed that the S. ...
... disadvantages of precipitin tests are twofold. First, tests may suffer from non-specificity in antibody binding or ... Precipitin tests have continued to be a popular way to detect host antigens in Triatominae blood meals (Christensen & de ... Specimens of seven additional species also tested positive for T. cruzi (Table 2), all of which were previously known to be ... Laboratory tests either offer insects a single possible host species and observe whether feeding occurs (SantAnna et al., 2001 ...
... pulmonary function tests (PFTs), immunologic tests, bronchial challenge testing). The results of some of these tests are ... Cormier Y, Letourneau L, Racine G. Significance of precipitins and asymptomatic lymphocytic alveolitis: a 20-year follow-up. ... pulmonary function testing, immediate hypersensitivity skin testing, delayed cutaneous hypersensitivity testing, ... Bronchial Challenge Testing: Sensitivity and Specificity. FIGURE 2: Positive result of a bronchial challenge test with pigeon ...
  • Serum precipitin tests were negative in both workers tested. (
  • SEDTION D) Although the chicken is a very good producer: of precipitins to native serum, proteins this immunological ability does not seem to extend to chemically altered antigens. (
  • So serum from that rabbit's blood can then be used as the test reagent. (
  • The key diagnostic tests are serum Aspergillus IgG testing, also known as Aspergillus precipitins, and radiology showing one or more cavities or nodules. (
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. (
  • There, it is examined for bands called precipitin that form when specific antibodies are present. (
  • The precipitin test helps check if the body has produced antibodies to a specific antigen, in this case, the Coccidioides fungus. (
  • It was pointed out that the cases of HP were not systematically identified, that MWF exposure was not measured, that HP prevalences and incidences were not estimated, and that no precipitating antibodies were found in the tested workers. (
  • Precipitin tests check for antibodies in your blood that recognize and bind to a specific substance. (
  • If you have antibodies to a substance, your healthcare provider may use an inhalation challenge test to confirm that this substance is causing your symptoms. (
  • ASO-Test identifies Streptolysin-O antibodies. (
  • One monoclonal precipitated an epimastigote antigen, while another reacted with a metacyclic antigen, and 2 antibodies formed precipitin lines with antigens of both stages. (
  • Aspergillus precipitin antibody test results (ie, for IgG) are usually positive. (
  • Antisera with precipitin titres of 1/2048 are readily obtained. (
  • It could be distinguished from all previously described Legionella species and serogroups by using antisera in slide agglutination tests. (
  • Rao 5 used agar precipitin tests and found six components in bovine lens with rabbit antisera. (
  • An improved method of measuring the amount of precipitum in connection with tests with precipitating antisera. (
  • IMSEAR at SEARO: A trial of haemagglutination, circumoval precipitin and gel diffusion tests in hookworm infection. (
  • Antibody to the T-catalase derived from a culture of Mycobacterium avium serovar 8 was purified by immunoaffinity absorption and elution and tested in a solid-phase immunosorbent assay against T-catalases from 31 strains in the M. avium complex. (
  • Serologic testing for Trypanosoma cruzi: comparison of radioimmunoprecipitation assay with commercially available indirect immunofluorescence assay, indirect hemagglutination assay, and enzyme-linked immunosorbent assay kits. (
  • Both systems were studied by carrying out quantitative precipitin tests and analysing the precipitates for total N and antigen N, Use of 131I-trace-labelled antigen in these tests made it possible to extend the analyses into the region of considerable antigen excess. (
  • Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e. (
  • Flocculation Tests: Precipitin tests which occur over a narrow range of antigen-antibody ratio, due chiefly to peculiarities of the antibody (precipitin). (
  • In single diffusion tests, antibody is incorporated into the gel and antigen is introduced into wells in the gel. (
  • Each antigen-antibody reaction forms an independent precipitation reaction, called a precipitin boundary. (
  • The precipitin reactions between the monoclonals and parasite extracts indicate that more than 1 identical epitope is present on each precipitating antigen molecule, while agglutinin analyses demonstrated antigenic specificities and relationships among stages, strains, and species of these trypanosomes. (
  • An antiserum to HCRSV from H rosa-sinensis reacted with the virus from A manihot to its homologous titre in precipitin tube tests, ELISA and immunosorbent electron microscopy, and was subsequently used in ELISA to detect infection in aibika. (
  • Occurs naturally in many species of wild and cultivated plants, and infects (often symptomlessly) a wide range of commonly used herbaceous test plants. (
  • They are oxidase positive and species can be identified by biochemical tests. (
  • The failure of 6-MP to suppress antibody formation in the chicken may be due to the particularly vigorous production of precipitins by this species or to a more rapid catabolism and excretion of the drug by the fowl. (
  • So the precipitin test had to be used to find out from which species all of these blood stains came from. (
  • The presence of immunoglobulins derived from species other than humans is excluded by precipitin tests. (
  • The sensitivity and specificity of the precipitin ring test and ELISA were compared, as well as the stability of the tests across different stages of blood meal digestion. (
  • The ELISA was more sensitive and specific than the precipitin test for identifying the sources of blood meals. (
  • When comparing the techniques across digestion stages, it was found that there was a drop in sensitivity, 48 and 72 h postblood meal for precipitin and visually read ELISA, respectively. (
  • Tube-precipitin or gel-diffusion tests give satisfactory results. (
  • The virus gives one line of precipitate in gel-diffusion tests. (
  • A single precipitin line is formed in gel diffusion tests. (
  • Often, identification of presumptive colonies is made simply by a combination positive oxidase reaction and serologic tests such as slide agglutination, coagglutination, precipitin reactions, or immunofluorescence staining. (
  • A sweat test using the Quantitative Pilocarpine Iontophoreseis Sweat Test Method is the first test to be performed in the evaluation. (
  • Takaki studied produce a positive wassermann test, although the precipitin, readily acted. (
  • The test should be performed in an accredited laboratory according to the National Committee for Clinical Lab Standards guidelines. (
  • The results of these immunologic tests must be interpreted in the context of the varied clinical presentations and the duration and clinical type of coccidioidomycosis. (
  • Immediate hypersensitivity skin testing is the most useful method to detect IgE antibody against mold allergens. (
  • Immediate hypersensitivity skin testing with bird sera and pigeon bloom was positive in 78% and 100% of BFL patients, respectively, and in 64% and 88% of control pigeon breeders, respectively. (
  • A case report of one worker, including clinical symptoms, work history, pulmonary function tests, and treatment was presented. (
  • Work related symptoms, initial pulmonary function tests and biopsy results were reported. (
  • The test is indicated in individuals who have clinically significant dermatographism or extensive skin disease, those who cannot discontinue antihistamines or other medications with antihistamine actions (eg, tricyclic antidepressants), or those who have a history of anaphylactic reaction (because direct application of the suspected allergen may precipitate recurrent anaphylaxis). (
  • These tests can help in identifying immediate hypersensitivity to suspected fungi. (
  • The history of immunology of lens proteins goes back to Uhlenhuth, 1 who 55 years ago showed by precipitation tests the organ specificity of some of the lens components. (
  • Some of the fractionation steps may contribute to the safety of immunoglobulins by inactivating or remov ing contaminating viruses that have not been detected by tests on the blood donations. (
  • The quality control of immunoglobulins includes potency tests and conventional tests for safety and sterility. (
  • Therefore, the use of recombinant Ag may help in reducing the inconsistency of test results with the use of natural Ags. (
  • Results are recorded 15 minutes after the test is performed. (
  • Your healthcare provider will diagnose hypersensitivity pneumonitis based on your medical history, symptoms, a physical exam, and results from lung tests and blood tests. (
  • Of four anti-rabbit CD18 Mabs tested, only one, L13/64, has been shown to be capable of inhibiting the adhesion of fMLP-stimulated neutrophils to gelatin coated plastic and the homotypic aggregation of PMA-stimulated T cells, both of which assays have been shown to be CD18-dependent. (
  • In this case, another test is done to confirm that you have an infection. (
  • [ 22 ] They found a statistically significant correlation in specific immunoglobulin E (IgE) levels to both Ags by using skin test and immunoblotting/inhibition analysis. (
  • Inhalation challenge tests show whether you develop symptoms when you breathe in a specific substance. (
  • and/or demonstration of a specific immunologic response (i.e., skin test conversion or demonstration of presence of coccidioidal antibody). (
  • Coccidioides precipitin is a blood test that looks for infections due to a fungus called Coccidioides, which causes the disease coccidioidomycosis or valley fever. (
  • SECTION C) Because the serological behaviour of chicken precipitating antibody differs antibody from that of typical mammalian antibody, it was necessary to undertake a preliminary investigation of the chicken anti-BSA and anti-BGG precipitin systems. (
  • A positive newborn screen should trigger evaluation in an accredited CF center and diagnostic testing for CF. (
  • Coccidioidal skin test conversion from negative to positive after the onset of clinical signs and symptoms. (
  • SECTION F) The effect of a cytotoxic drug, 6-mercaptopicrine on precipitin production in the adult fowl, was investigated. (
  • One antibody agglutinated all stages and strains of T. cruzi, T. musculi , and Trypanosoma lewisi which were tested. (
  • Mold allergen extracts used for the skin test depend on the prevalence of various molds in the region, as identified with annual atmospheric sampling. (
  • This test is most critical for establishing the diagnosis. (
  • If there is an interaction between the antibody and the sample, when the two meet, then there will be a visible precipitin line in the gel. (
  • The sweat glands produce sweat with an elevated concentration of chloride and sodium, so measurement of sweat chloride concentration is the key diagnostic test. (
  • The diagnosis of fungal sensitivity heavily depends on skin tests with fungal allergens. (
  • Since only a limited panel of CF mutations is routinely tested, the absence of CF mutations does not exclude the diagnosis of CF. (
  • In addition to the safety and sterility tests, total protein is determined by nitrogen estimations, the protein composition by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and molecular size by high performance liquid chromatography. (
  • This means the blood test did not detect the antibody to Coccidioides. (
  • Testing can be performed by using the prick or intradermal method. (