Podospora
Xylariales
Sordariales
Ascomycota
DNA, Mitochondrial
Genes, Mating Type, Fungal
Molecular Sequence Data
Fungi
The sequences appended to the amyloid core region of the HET-s prion protein determine higher-order aggregate organization in vivo. (1/78)
The [Het-s] prion of the fungus Podospora anserina propagates as a self-perpetuating amyloid form of the HET-s protein. This protein triggers a cell death reaction termed heterokaryon incompatibility when interacting with the HET-S protein, an allelic variant of HET-s. HET-s displays two distinct domains, a N-terminal globular domain and a C-terminal unstructured prion-forming domain (residues 218-289). Here, we describe the characterization of HET-s(157-289), a truncated form of HET-s bearing an extensive deletion in the globular domain but retaining full activity in incompatibility and prion propagation. In vitro, HET-s(157-289) polymerizes into amyloid fibers displaying the same core region as full-length HET-s fibers. We have shown previously that fusions of green fluorescent protein (GFP) with HET-s or HET-s(218-289) form dot-like aggregates in vivo upon transition to the prion state. By contrast, a HET-s(157-289)/GFP fusion protein forms elongated fibrillar aggregates in vivo. Such elongated aggregates can reach up to 150 microm in length. The in vivo dynamics of these organized structures is analysed by time lapse microscopy. We find that the large elongate structures grow by lateral association of shorter fibrillar aggregates. When co-expressed with HET-s(157-289), full-length HET-s and HET-s(218-289) can be incorporated into such elongated aggregates. Together, our data indicate that HET-s(157-289) aggregates can adopt an organized higher-order structure in vivo and that the ability to adopt this supramolecular organization is conferred by the sequences appended to the amyloid core region. (+info)Altering a gene involved in nuclear distribution increases the repeat-induced point mutation process in the fungus Podospora anserina. (2/78)
Repeat-induced point mutation (RIP) is a homology-dependent gene-silencing mechanism that introduces C:G-to-T:A transitions in duplicated DNA segments. Cis-duplicated sequences can also be affected by another mechanism called premeiotic recombination (PR). Both are active over the sexual cycle of some filamentous fungi, e.g., Neurospora crassa and Podospora anserina. During the sexual cycle, several developmental steps require precise nuclear movement and positioning, but connections between RIP, PR, and nuclear distributions have not yet been established. Previous work has led to the isolation of ami1, the P. anserina ortholog of the Aspergillus nidulans apsA gene, which is required for nuclear positioning. We show here that ami1 is involved in nuclear distribution during the sexual cycle and that alteration of ami1 delays the fruiting-body development. We also demonstrate that ami1 alteration affects loss of transgene functions during the sexual cycle. Genetically linked multiple copies of transgenes are affected by RIP and PR much more frequently in an ami1 mutant cross than in a wild-type cross. Our results suggest that the developmental slowdown of the ami1 mutant during the period of RIP and PR increases time exposure to the duplication detection system and thus increases the frequency of RIP and PR. (+info)Understanding aging: revealing order out of chaos. (3/78)
Aging is often described as an extremely complex process affecting all of the vital parameters of an individual. In this article, we review how understanding of aging evolved from the first analyses of population survival to the identification of the molecular mechanisms regulating life span. Abundant evidence implicates mitochondria in aging and we focus on the three main components of the mitochondrial theory of aging: (1) increased reactive oxygen species (ROS) production, (2) mitochondrial DNA (mtDNA) damage accumulation, and (3) progressive respiratory chain dysfunction. Experimental evidence shows a relationship between respiratory chain dysfunction, ROS damage, and aging in most of the model organisms. However, involvement of the mtDNA mutations in the aging process is still debated. We recently created a mutant mouse strain with increased levels of somatic mtDNA mutations causing a progressive respiratory chain deficiency and premature aging. These mice demonstrate the fundamental importance of the accumulation of mtDNA alterations in aging. We present here an integrative model where aging is provoked by a single primary event leading to a variety of effects and secondary causes. (+info)Interaction between the oxa1 and rmp1 genes modulates respiratory complex assembly and life span in Podospora anserina. (4/78)
A causal link between deficiency of the cytochrome respiratory pathway and life span was previously shown in the filamentous fungus Podospora anserina. To gain more insight into the relationship between mitochondrial function and life span, we have constructed a strain carrying a thermosensitive mutation of the gene oxa1. OXA1 is a membrane protein conserved from bacteria to human. The mitochondrial OXA1 protein is involved in the assembly/insertion of several respiratory complexes. We show here that oxa1 is an essential gene in P. anserina. The oxa1(ts) mutant exhibits severe defects in the respiratory complexes I and IV, which are correlated with an increased life span, a strong induction of the alternative oxidase, and a reduction in ROS production. However, there is no causal link between alternative oxidase level and life span. We also show that in the oxa1(ts) mutant, the extent of the defects in complexes I and IV and the life-span increase depends on the essential gene rmp1. The RMP1 protein, whose function is still unknown, can be localized in the mitochondria and/or the cytosolic compartment, depending on the developmental stage. We propose that the RMP1 protein could be involved in the process of OXA1-dependent protein insertion. (+info)Probing the structure of the infectious amyloid form of the prion-forming domain of HET-s using high resolution hydrogen/deuterium exchange monitored by mass spectrometry. (5/78)
The HET-s prion protein of Podospora anserina represents a valuable model system to study the structural basis of prion propagation. In this system, prion infectivity can be generated in vitro from a recombinant protein. We have previously identified the region of the HET-s protein involved in amyloid formation and prion propagation. Herein, we show that a recombinant peptide corresponding to the C-terminal prion-forming domain of HET-s (residues 218-289) displays infectivity. We used high resolution hydrogen/deuterium exchange analyzed by mass spectrometry to gain insight into the structural organization of this infectious amyloid form of the HET-s-(218-289) protein. Deuterium incorporation was analyzed by ion trap mass spectrometry for 76 peptides generated by pepsin proteolysis of HET-s-(218-289). By taking into account sequence overlaps in these peptides, a resolution ranging from 4-amino acids stretches to a single residue could be achieved. This approach allowed us to define highly protected regions alternating with more accessible segments along the HET-s-(218-289) sequence. The HET-s-(218-289) fibrils are thus likely to be organized as a succession of beta-sheet segments interrupted by short turns or short loops. (+info)The function of the coding sequences for the putative pheromone precursors in Podospora anserina is restricted to fertilization. (6/78)
We cloned the pheromone precursor genes of Podospora anserina in order to elucidate their role in the biology of this fungus. The mfp gene encodes a 24-amino-acid polypeptide finished by the CAAX motif, characteristic of fungal lipopeptide pheromone precursors similar to the a-factor precursor of Saccharomyces cerevisiae. The mfm gene encodes a 221-amino-acid polypeptide, which is related to the S. cerevisiae alpha-factor precursor and contains two 13-residue repeats assumed to correspond to the mature pheromone. We deleted the mfp and mfm coding sequence by gene replacement. The mutations specifically affect male fertility, without impairing female fertility and vegetative growth. The male defect is mating type specific: the mat+ Deltamfp and mat- Deltamfm mutants produce male cells inactive in fertilization whereas the mat- Deltamfp and mat+ Deltamfm mutants show normal male fertility. Genetic data indicate that both mfp and mfm are transcribed at a low level in mat+ and mat- vegetative hyphae. Northern-blot analysis shows that their transcription is induced by the mating types in microconidia (mfp by mat+ and mfm by mat-). We managed to cross Deltamfp Deltamfm strains of opposite mating type, by complementation and transient expression of the pheromone precursor gene to trigger fertilization. These crosses were fertile, demonstrating that once fertilization occurs, the pheromone precursor genes are unnecessary for the completion of the sexual cycle. Finally, we show that the constitutively transcribed gpd::mfm and gpd::mfp constructs are repressed at a posttranscriptional level by the noncognate mating type. (+info)DNA-binding specificity of the IDI-4 basic leucine zipper factor of Podospora anserina defined by systematic evolution of ligands by exponential enrichment (SELEX). (7/78)
Heterokaryon incompatibility is a cell destruction process that occurs when fungal cells of unlike genotype fuse. In Podospora anserina, autophagy is engaged during cell death by incompatibility and a number of genes are induced at the transcriptional level. These genes are termed idi (induced during incompatibility) genes. Among these is idi-4, a gene encoding a basic leucine zipper (bZIP) factor. IDI-4 displays similarity to the GCN4/cross-pathway control (CPC) factors that control gene expression in response to amino acid starvation in fungi. The overexpression of idi-4 triggers autophagy, leads to cell death, and also increases the expression of a number of idi genes, in particular idi-7, a gene involved in autophagy. Herein, we determined the in vitro target sequence of IDI-4. We have purified the recombinant IDI-4 bZIP domain and show that this 83-amino-acid-long peptide dimerizes in vitro and adopts an alpha-helical fold. We have then used a systematic evolution of ligands by exponential enrichment procedure to identify the sequence bound by the IDI-4 bZIP domain. The IDI-4 binding site consensus sequence corresponds to the ATGANTCAT pseudopalindrome. IDI-4 binding sites are present in the promoter region of the idi-7 gene, and the bZIP IDI-4 peptide binds to the idi-7 promoter in vitro. The identified IDI-4 consensus binding sequence is very similar to the GCN4/CPC binding site, raising the possibility of an interplay and/or partial functional redundancy between IDI-4 and CPC-type bZIP factors in fungi. (+info)Correlation of structural elements and infectivity of the HET-s prion. (8/78)
Prions are believed to be infectious, self-propagating polymers of otherwise soluble, host-encoded proteins. This concept is now strongly supported by the recent findings that amyloid fibrils of recombinant prion proteins from yeast, Podospora anserina and mammals can induce prion phenotypes in the corresponding hosts. However, the structural basis of prion infectivity remains largely elusive because acquisition of atomic resolution structural properties of amyloid fibrils represents a largely unsolved technical challenge. HET-s, the prion protein of P. anserina, contains a carboxy-terminal prion domain comprising residues 218-289. Amyloid fibrils of HET-s(218-289) are necessary and sufficient for the induction and propagation of prion infectivity. Here, we have used fluorescence studies, quenched hydrogen exchange NMR and solid-state NMR to determine the sequence-specific positions of amyloid fibril secondary structure elements of HET-s(218-289). This approach revealed four beta-strands constituted by two pseudo-repeat sequences, each forming a beta-strand-turn-beta-strand motif. By using a structure-based mutagenesis approach, we show that this conformation is the functional and infectious entity of the HET-s prion. These results correlate distinct structural elements with prion infectivity. (+info)"Podospora" is not a term that has a medical definition. It is a genus of fungi in the order of Hypocreales, which are commonly found in soil and decaying organic matter. Some species of Podospora are known to produce perithecia, a type of sexual fruiting body, and ascospores, which are used for reproduction.
While Podospora fungi themselves do not have direct medical relevance, it is worth noting that some fungi can cause infections in humans, particularly in individuals with weakened immune systems. However, Podospora species are not typically associated with human disease.
Xylariales is an order of fungi in the class Sordariomycetes, which are primarily wood-inhabiting species. This group includes both saprobic and pathogenic fungi, with some members known to cause various plant diseases. The order contains several families, including Xylariaceae, Amphisphaeriaceae, and Graphostromataceae, among others. Many species in Xylariales produce dark-colored, melanized structures called pycnidia or stromata, which contain the reproductive structures of the fungi. Some members of this order also have potential industrial applications, such as the production of enzymes and bioactive compounds.
Sordariales is an order of fungi in the class Sordariomycetes, which are characterized by their perithecial ascomata (sexual fruiting bodies) with cylindrical to allantoid (sausage-shaped) asci (ascus = sac-like structure containing ascospores). The order includes both saprobic and pathogenic species. Some notable members of Sordariales are:
1. Neurospora crassa - A model organism used in genetics, molecular biology, and biochemistry research. It is a filamentous ascomycete fungus with a worldwide distribution and is commonly found on decaying plant material.
2. Sordaria fimicola - Another model organism for genetic studies, particularly in the field of meiosis and genetics of sexual reproduction.
3. Chaetomium globosum - A saprobic fungus that can be isolated from various substrates such as soil, dung, and decaying plant material. It is also known to cause opportunistic infections in humans.
4. Xylaria hypoxylon - A wood-decay fungus commonly found on dead or dying trees and branches. Some species are capable of causing rot in living plants.
5. Graphostroma platystomum - A pathogenic fungus that causes canker diseases in various tree species, such as oak, beech, and chestnut.
The order Sordariales is primarily defined by its unique ascus morphology and the presence of certain molecular markers, such as specific genes related to the sexual reproduction process.
Ascomycota is a phylum in the kingdom Fungi, also known as sac fungi. This group includes both unicellular and multicellular organisms, such as yeasts, mold species, and morel mushrooms. Ascomycetes are characterized by their reproductive structures called ascus, which contain typically eight haploid spores produced sexually through a process called ascogony. Some members of this phylum have significant ecological and economic importance, as they can be decomposers, mutualistic symbionts, or plant pathogens causing various diseases. Examples include the baker's yeast Saccharomyces cerevisiae, ergot fungus Claviceps purpurea, and morel mushroom Morchella esculenta.
Fungal proteins are a type of protein that is specifically produced and present in fungi, which are a group of eukaryotic organisms that include microorganisms such as yeasts and molds. These proteins play various roles in the growth, development, and survival of fungi. They can be involved in the structure and function of fungal cells, metabolism, pathogenesis, and other cellular processes. Some fungal proteins can also have important implications for human health, both in terms of their potential use as therapeutic targets and as allergens or toxins that can cause disease.
Fungal proteins can be classified into different categories based on their functions, such as enzymes, structural proteins, signaling proteins, and toxins. Enzymes are proteins that catalyze chemical reactions in fungal cells, while structural proteins provide support and protection for the cell. Signaling proteins are involved in communication between cells and regulation of various cellular processes, and toxins are proteins that can cause harm to other organisms, including humans.
Understanding the structure and function of fungal proteins is important for developing new treatments for fungal infections, as well as for understanding the basic biology of fungi. Research on fungal proteins has led to the development of several antifungal drugs that target specific fungal enzymes or other proteins, providing effective treatment options for a range of fungal diseases. Additionally, further study of fungal proteins may reveal new targets for drug development and help improve our ability to diagnose and treat fungal infections.
Fungal genes refer to the genetic material present in fungi, which are eukaryotic organisms that include microorganisms such as yeasts and molds, as well as larger organisms like mushrooms. The genetic material of fungi is composed of DNA, just like in other eukaryotes, and is organized into chromosomes located in the nucleus of the cell.
Fungal genes are segments of DNA that contain the information necessary to produce proteins and RNA molecules required for various cellular functions. These genes are transcribed into messenger RNA (mRNA) molecules, which are then translated into proteins by ribosomes in the cytoplasm.
Fungal genomes have been sequenced for many species, revealing a diverse range of genes that encode proteins involved in various cellular processes such as metabolism, signaling, and regulation. Comparative genomic analyses have also provided insights into the evolutionary relationships among different fungal lineages and have helped to identify unique genetic features that distinguish fungi from other eukaryotes.
Understanding fungal genes and their functions is essential for advancing our knowledge of fungal biology, as well as for developing new strategies to control fungal pathogens that can cause diseases in humans, animals, and plants.
Fungal DNA refers to the genetic material present in fungi, which are a group of eukaryotic organisms that include microorganisms such as yeasts and molds, as well as larger organisms like mushrooms. The DNA of fungi, like that of all living organisms, is made up of nucleotides that are arranged in a double helix structure.
Fungal DNA contains the genetic information necessary for the growth, development, and reproduction of fungi. This includes the instructions for making proteins, which are essential for the structure and function of cells, as well as other important molecules such as enzymes and nucleic acids.
Studying fungal DNA can provide valuable insights into the biology and evolution of fungi, as well as their potential uses in medicine, agriculture, and industry. For example, researchers have used genetic engineering techniques to modify the DNA of fungi to produce drugs, biofuels, and other useful products. Additionally, understanding the genetic makeup of pathogenic fungi can help scientists develop new strategies for preventing and treating fungal infections.
Mitochondrial DNA (mtDNA) is the genetic material present in the mitochondria, which are specialized structures within cells that generate energy. Unlike nuclear DNA, which is present in the cell nucleus and inherited from both parents, mtDNA is inherited solely from the mother.
MtDNA is a circular molecule that contains 37 genes, including 13 genes that encode for proteins involved in oxidative phosphorylation, a process that generates energy in the form of ATP. The remaining genes encode for rRNAs and tRNAs, which are necessary for protein synthesis within the mitochondria.
Mutations in mtDNA can lead to a variety of genetic disorders, including mitochondrial diseases, which can affect any organ system in the body. These mutations can also be used in forensic science to identify individuals and establish biological relationships.
Mycelium is not a specifically medical term, but it is a biological term used in fungi and other organisms. Medically, it might be relevant in certain contexts such as discussing fungal infections. Here's the general definition:
Mycelium (my-SEE-lee-um) is the vegetative part of a fungus, consisting of a mass of branching, thread-like hyphae. It is the underground portion of the fungus that supports the growth of the organism and is often responsible for the decomposition of organic material. Mycelium can be found in various environments, including soil, water, and dead or living organisms.
1. Genes: These are hereditary units that carry genetic information from parents to offspring and determine various characteristics such as eye color, hair color, and height in living organisms. In fungi, genes are responsible for encoding different traits, including mating type.
2. Mating Type: Fungi have a complex sexual reproduction system involving two or more mating types that must come together to reproduce sexually. The mating type of a fungus is determined by the presence or absence of specific genes called "mating type loci" (MAT). These genes control the ability of fungal cells to recognize and fuse with each other during sexual reproduction.
3. Fungal: This term refers to any member of the kingdom Fungi, which includes a diverse group of organisms such as yeasts, molds, and mushrooms. Fungi are eukaryotic, meaning they have complex cells with a true nucleus and other membrane-bound organelles. They play essential roles in various ecosystems, decomposing organic matter, recycling nutrients, and forming mutualistic relationships with plants and animals.
In summary, 'Genes, Mating Type, Fungal' refers to the genetic factors that determine the mating type of fungi, which is crucial for their sexual reproduction and survival in various environments.
Fungal spores are defined as the reproductive units of fungi that are produced by specialized structures called hyphae. These spores are typically single-celled and can exist in various shapes such as round, oval, or ellipsoidal. They are highly resistant to extreme environmental conditions like heat, cold, and dryness, which allows them to survive for long periods until they find a suitable environment to germinate and grow into a new fungal organism. Fungal spores can be found in the air, water, soil, and on various surfaces, making them easily dispersible and capable of causing infections in humans, animals, and plants.
Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.
Fungi, in the context of medical definitions, are a group of eukaryotic organisms that include microorganisms such as yeasts and molds, as well as the more familiar mushrooms. The study of fungi is known as mycology.
Fungi can exist as unicellular organisms or as multicellular filamentous structures called hyphae. They are heterotrophs, which means they obtain their nutrients by decomposing organic matter or by living as parasites on other organisms. Some fungi can cause various diseases in humans, animals, and plants, known as mycoses. These infections range from superficial, localized skin infections to systemic, life-threatening invasive diseases.
Examples of fungal infections include athlete's foot (tinea pedis), ringworm (dermatophytosis), candidiasis (yeast infection), histoplasmosis, coccidioidomycosis, and aspergillosis. Fungal infections can be challenging to treat due to the limited number of antifungal drugs available and the potential for drug resistance.
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